LOC100496327 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100496327
Entrez Gene ID	100496327
Full Name	myosin-11-like
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012966921.1	XP_012822375.1	myosin-11-like isoform X2
XM_002938880.3	XP_002938926.2	myosin-11-like isoform X1
XM_002938880.4	XP_002938926.3	myosin-11 isoform X1
XM_031899137.1	XP_031754997.1	rho-associated protein kinase 1 isoform X3
XM_012966921.2	XP_012822375.2	myosin-11 isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC101884668	XP_005163198.1
Xenopus tropicalis (tropical clawed frog)	LOC100496327	XP_002938926.2

Gene Ontology

The following LOC100496327 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100496327 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa55016	XM_002938880.4 Latest version!	Xenopus tropicalis rho-associated protein kinase 1 (LOC100496327), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa55017	XM_012966921.2 Latest version!	Xenopus tropicalis rho-associated protein kinase 1 (LOC100496327), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa55018	XM_031899137.1 Latest version!	Xenopus tropicalis rho-associated protein kinase 1 (LOC100496327), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa13783	XM_002938880.3 Latest version!	Xenopus tropicalis myosin-11-like (LOC100496327), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa36524	XM_012966921.1 Latest version!	Xenopus tropicalis myosin-11-like (LOC100496327), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100496327 ( XM_002938880.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002938926.3 Xenopus tropicalis rho-associated protein kinase 1 (LOC100496327), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa55016
Clone ID Related Accession (Same CDS sequence)	XM_002938880.4
Accession Version	XM_002938880.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	myosin-11 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002938880.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTATA GAGTGTGTAA CGGTTATGTG AGTTTGCCCT CCCGGTATGG AACAACTGGG 
AACTCTCAGT ATCATGTTGA CAGCGGCAGG ATATCTCCTT GCTGGTCCTA CAAAGATCTT 
GGAAGCAGCA GACATGGTTT GGAGTTATCC AAATCCCCCG TTTTATCTGA CCTAGAGAAA 
AATATTGAGA CAGCGGTGAA TTTAGAATCT AGCTTTCTGC CTGGGCAATG GAAAGATTAC 
AGCAGGAGTT CAGTAAGTCC ACATCATTCA GAGAGAGCGA GGTCTACAAG TCCAATGCGG 
GGGATCCACT CTCTAGAATG TTCCAGAATC CAGTCCACTC CTCCCATTTC AAGAAGAAAT 
TCACTTTCCC GTCTACCAAC TTCATCTTCT GAGGATTTCA GTCTTGAGCT GCCATTGAGC 
CGCAGAGGGT GGAGTGGGTC AGAGTTGGAT CTAAAGGCAT CATTGACTGA AAGTGCCCAA 
AAGCGGGCTG ATCTGGTGCA GAGACTGAAA GATGCTCAGG TGAAGCTGGA GGGCCAATCA 
GAGGAGCTGC GGCACCGGGA TAAGGAGCTG GAGTTCAGCA GAGCTAAAAT TGAATTACTG 
GCAGTGAAGC AAAAGCAACT GGAAACATCA ATATCTCAGT TAGAGAAGGA AAAGGGTTGG 
TTAGAGGTGT CCCGCTATGA GGATAAGAGG CAACGTGGTG AACTGCAAGA CCGGATTATT 
AATCTAGAAA TGGAAGTGAT GAAGACCAAA TCTAACCTGG AAAATCTTAA CTACACCAGT 
GTACTAAAGT ATCAGAAGAC TCCAATGGAC TTAAAGGAAA ATGATGAACT AAGTCGAGAA 
CTAAGAACTG CACGTGAGAA TCTACTCTAC CTTCGAAATC GAGTCAAAGT CCTGGACGCA 
GAGAAGACTC AATCTGTTGA GGAACTAAGG ATATTGAGAG AGGGGAGTCA GCAGGCCTTT 
AGCCAAACCA ATGATGCAAA CCAGAGGGTA ACAGACACAC TCCGGGCTCA AAAAGATCTG 
CAAGAAGAGC TGGCAAATCT GCAACTGAAC CTAAACACTA CCAGTCTGGA GAAGGAGCTC 
GTGTCCTCAA AGATTATGAG ATTGGAGGAA AAGGTTAAAG ATCTGAATTT AAGGCTAAAT 
ATTGCACATT CTGACAGGGA ACGGGTTCTG CAGGACAAGG TGGAGCTGAC TAGGCGAAAT 
CAGGAATTGT CTCTCGAACT AGAACGGGCT CAGAGGGGTC AGGAGGGGTT TAATGACCAG 
GTCAGTGACC TTCACATTGA GCTGGTAGGA GCTAAAGCCC AGTCAAACCG TCAGGACCAG 
GAGAAGGTTC AGATGAAGGA GGAGCTGGTG ATGCTGAAAC AGGTGAATGA GAAACTGACT 
GCTGAACTGG GACAGACACA GCATACACTC CAGCGCACCC TGGATGAACT GCATCAGCTC 
CAGGCAGAAC ACAAGATTTC AACCAACCTT ACAGCTGCCT TGGAGGCAGA ACGTTCCCAG 
CTCTTGGGTG AGAAAGAGCT GCTGATGTCA ACTGTGGAGG AACATGGGGA GAGCCAGAGC 
ATCCAAGAGC TCAGATCTTC TCAGGCGCAG TTAATAGAAG AGAGGGATAA CCTACGTACC 
CATTGCCAGA ACCTGCAGGT TTCACTGGAA CAAGCACATG AACAGCTCGG CTCCCAGATC 
CAGGAGCAGC AGCAGCTCAC TCTGTATTGG AAGGAAAGGT GGCAGCAGAC TGCTGTTAAT 
TTAAAAAGCA CAGAAGAACA ACTGGAACAG GCAAAGGCTC TTTGCTCTAG TACAACAAAT 
AAGCACTCTG AGATCCTCCA AGATTATGAA CTCCTACAAA CAGATGCAGA GGAGCTGGCT 
GAACTCAGGA TGGCTATCAG CAGCCTGAAG AAAGAAAACA GCCTTTTAAA AGAGCAGATA 
AAGGAGCAGG AACAGAACAA GCATCTGCAG TGTCTTCAGA GCCACATCCA GGCTAATGTG 
GACAGCCCAG AGGATATGAA TTCGAAACTT GGCAAGCTGT ATAAAGAATT ACAGCAGGCT 
CACGAAAGGT TAAAATTCTT GGAGAGAGAA AGGTCTGAAA AAGAACTGGA AATAAAAAAA 
CTTAAGGCTG AGAGTGGATC CATACTTCGC ATTGAGCTAG ATGCCTGCAA ACAACAGTTG 
GAATTGGAGA GAAGTCGAAC AGCGACTCTA CAGGCCAAGA TTTCAGTCAT GGATTCCAGA 
AACAAAATGG AGGTTGCAAG TAGTCAGGAT CGTGTAGACA GTTTGCAGAA CCACAGCTTG 
TCCCTTCTAT CAGAAAGGTT AACATCAGAC GAATTGAGTT CTGAGATTGA GAATCTTCGT 
CAGCAGCTAG AAGAGGAGAG AGATGCAAAA AAGAAGAAAG AAGAACTGAT ATTTAATCTA 
AAAGAAGAGC TTGATGATCT CAAGCACAAG AAGCCTGGAG AAATCAAGGC ATCTCTGGAG 
GAAGTGGACT CAGAGCTAAT GTTTGTGCGA GAAGAACTGC AGAAAGTCTG GGAAATGTTG 
AAGACAAAAG ATACAGAACT TGAGGAACAG TATCAGGAGC TGGAGTCTGC TCGAGGCCAG 
TACACAGAGT GCAGCACAGA AAAAATGCGA CTGGAGCAAG TTCTTGCCAC CTTGCAGCAG 
CAGTTGACTG AGAAAGAGCA GACAATGAAA CATCTCAAGC AAATGAGAGA AATGGAGAAA 
ACTGAAATGG ATATTGAGAG GTCCTCATTG CAACTGAAGC TGGCAGAGCT ACAAGGACAA 
AAAGGAGATG TCACAACTCA GGCTGGGCAG CCGATAGAGG AGACTGGGCC TAAATGGTGG 
CGTTCAAAGG GAATGAAGGT TGTAGAGTCA TCAAAGAAAT GTATTCGCTG TGAAGCATTT 
TTGCAACAGT TGGATGAGAC AATCAAGGGT TGCCAGGAAC ACAATGCCGA GTTACAAGAA 
GAGAAGAACC AAACCTTGGC ATCCCTATAC CAGTTACAAG AAGTCCTAAC AAACCTCTCA 
AAACAATCAA AAGTAAATGA ACACGTGGCC CAGAGTCTAC AGGCGGACAA TGACTCTCTG 
AAAAAACAAC ACAGACTGGT GACAGAACAG CTTAAATGTC TGTTCAAAGA GAAAGAATCT 
CTGGGAAAAG CCTACCATAA GCTTCCAAAG AAGGATAAGC CAACGGAGGA CTGGAATGTT 
CAGTCCAGAT TAGTAAAGAA TATGCTGAGT TCTATAAAAA GCCATAAAAA CCAGCAAGAA 
GAGCTAACAG AAGAGGCAGG CCATCAACCA ATGGAATTCG ATGCTGTGCA GATGGAAACT 
AATGAAAGAG ACATGAGGAA CTTGCAAAGA CAGCTTGAGG AAAAGTCTGA GCAGATATTG 
AAAATGGCTT CAGAAATGAA ACTGTTACAA GAAAAAAATG AGTCTTTAAT GAAAGCTAAG 
ATGCGTTTTC AACAACAAGT CCAGCAAATT CGGAGCATGC CACATCTAAG TCATCAAAGA 
GAAAAGACAG ATCCTACTGT ACCACCTCTG TCTGGAGACA TCAACACCTC CTTTCACAAA 
AAGAATGAGT TTTTGGCAGC AGAGAGCCAA GATTGTTCTG GTAGAATGCA GGGAACACCA 
ACTTCATTTC AGAGTGCCTC AGAGGGGTCC TGGACATCAT CACCTCAAAA CTCAAGACCT 
CCAACACCCA TCAACCAAAA GGTTATGCCA TCTGCATCTT TACTGAGAGT TCAGCTTCCT 
GGCACAGTGG GGAAACACTG GGTAAAATCA AAAGTGGAAT CTCCTTTAAG CCCCAGAAGC 
CTAGCAGACA GTGAAGGTAA CAGTGATACT CCAGGGTCAG TTACTCCAAG GACTTCTGCA 
TTGCTGTCTC CCAGACCTTT CCATTCACCA AAATCGGCAC AGGCCTTCAG ATATGGAAAG 
AATGTGCAAC ACACAAACAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002938926.3
CDS	285..4208
Translation	MSYRVCNGYVSLPSRYGTTGNSQYHVDSGRISPCWSYKDLGSSRHGLELSKSPVLSDLEKNIETAVNLESSFLPGQWKDYSRSSVSPHHSERARSTSPMRGIHSLECSRIQSTPPISRRNSLSRLPTSSSEDFSLELPLSRRGWSGSELDLKASLTESAQKRADLVQRLKDAQVKLEGQSEELRHRDKELEFSRAKIELLAVKQKQLETSISQLEKEKGWLEVSRYEDKRQRGELQDRIINLEMEVMKTKSNLENLNYTSVLKYQKTPMDLKENDELSRELRTARENLLYLRNRVKVLDAEKTQSVEELRILREGSQQAFSQTNDANQRVTDTLRAQKDLQEELANLQLNLNTTSLEKELVSSKIMRLEEKVKDLNLRLNIAHSDRERVLQDKVELTRRNQELSLELERAQRGQEGFNDQVSDLHIELVGAKAQSNRQDQEKVQMKEELVMLKQVNEKLTAELGQTQHTLQRTLDELHQLQAEHKISTNLTAALEAERSQLLGEKELLMSTVEEHGESQSIQELRSSQAQLIEERDNLRTHCQNLQVSLEQAHEQLGSQIQEQQQLTLYWKERWQQTAVNLKSTEEQLEQAKALCSSTTNKHSEILQDYELLQTDAEELAELRMAISSLKKENSLLKEQIKEQEQNKHLQCLQSHIQANVDSPEDMNSKLGKLYKELQQAHERLKFLERERSEKELEIKKLKAESGSILRIELDACKQQLELERSRTATLQAKISVMDSRNKMEVASSQDRVDSLQNHSLSLLSERLTSDELSSEIENLRQQLEEERDAKKKKEELIFNLKEELDDLKHKKPGEIKASLEEVDSELMFVREELQKVWEMLKTKDTELEEQYQELESARGQYTECSTEKMRLEQVLATLQQQLTEKEQTMKHLKQMREMEKTEMDIERSSLQLKLAELQGQKGDVTTQAGQPIEETGPKWWRSKGMKVVESSKKCIRCEAFLQQLDETIKGCQEHNAELQEEKNQTLASLYQLQEVLTNLSKQSKVNEHVAQSLQADNDSLKKQHRLVTEQLKCLFKEKESLGKAYHKLPKKDKPTEDWNVQSRLVKNMLSSIKSHKNQQEELTEEAGHQPMEFDAVQMETNERDMRNLQRQLEEKSEQILKMASEMKLLQEKNESLMKAKMRFQQQVQQIRSMPHLSHQREKTDPTVPPLSGDINTSFHKKNEFLAAESQDCSGRMQGTPTSFQSASEGSWTSSPQNSRPPTPINQKVMPSASLLRVQLPGTVGKHWVKSKVESPLSPRSLADSEGNSDTPGSVTPRTSALLSPRPFHSPKSAQAFRYGKNVQHTNS

Target ORF information:

RefSeq Version	XM_002938880.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis rho-associated protein kinase 1 (LOC100496327), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002938880.4

ORF Insert Sequence:

ATGTCTTATA GAGTGTGTAA CGGTTATGTG AGTTTGCCCT CCCGGTATGG AACAACTGGG 
AACTCTCAGT ATCATGTTGA CAGCGGCAGG ATATCTCCTT GCTGGTCCTA CAAAGATCTT 
GGAAGCAGCA GACATGGTTT GGAGTTATCC AAATCCCCCG TTTTATCTGA CCTAGAGAAA 
AATATTGAGA CAGCGGTGAA TTTAGAATCT AGCTTTCTGC CTGGGCAATG GAAAGATTAC 
AGCAGGAGTT CAGTAAGTCC ACATCATTCA GAGAGAGCGA GGTCTACAAG TCCAATGCGG 
GGGATCCACT CTCTAGAATG TTCCAGAATC CAGTCCACTC CTCCCATTTC AAGAAGAAAT 
TCACTTTCCC GTCTACCAAC TTCATCTTCT GAGGATTTCA GTCTTGAGCT GCCATTGAGC 
CGCAGAGGGT GGAGTGGGTC AGAGTTGGAT CTAAAGGCAT CATTGACTGA AAGTGCCCAA 
AAGCGGGCTG ATCTGGTGCA GAGACTGAAA GATGCTCAGG TGAAGCTGGA GGGCCAATCA 
GAGGAGCTGC GGCACCGGGA TAAGGAGCTG GAGTTCAGCA GAGCTAAAAT TGAATTACTG 
GCAGTGAAGC AAAAGCAACT GGAAACATCA ATATCTCAGT TAGAGAAGGA AAAGGGTTGG 
TTAGAGGTGT CCCGCTATGA GGATAAGAGG CAACGTGGTG AACTGCAAGA CCGGATTATT 
AATCTAGAAA TGGAAGTGAT GAAGACCAAA TCTAACCTGG AAAATCTTAA CTACACCAGT 
GTACTAAAGT ATCAGAAGAC TCCAATGGAC TTAAAGGAAA ATGATGAACT AAGTCGAGAA 
CTAAGAACTG CACGTGAGAA TCTACTCTAC CTTCGAAATC GAGTCAAAGT CCTGGACGCA 
GAGAAGACTC AATCTGTTGA GGAACTAAGG ATATTGAGAG AGGGGAGTCA GCAGGCCTTT 
AGCCAAACCA ATGATGCAAA CCAGAGGGTA ACAGACACAC TCCGGGCTCA AAAAGATCTG 
CAAGAAGAGC TGGCAAATCT GCAACTGAAC CTAAACACTA CCAGTCTGGA GAAGGAGCTC 
GTGTCCTCAA AGATTATGAG ATTGGAGGAA AAGGTTAAAG ATCTGAATTT AAGGCTAAAT 
ATTGCACATT CTGACAGGGA ACGGGTTCTG CAGGACAAGG TGGAGCTGAC TAGGCGAAAT 
CAGGAATTGT CTCTCGAACT AGAACGGGCT CAGAGGGGTC AGGAGGGGTT TAATGACCAG 
GTCAGTGACC TTCACATTGA GCTGGTAGGA GCTAAAGCCC AGTCAAACCG TCAGGACCAG 
GAGAAGGTTC AGATGAAGGA GGAGCTGGTG ATGCTGAAAC AGGTGAATGA GAAACTGACT 
GCTGAACTGG GACAGACACA GCATACACTC CAGCGCACCC TGGATGAACT GCATCAGCTC 
CAGGCAGAAC ACAAGATTTC AACCAACCTT ACAGCTGCCT TGGAGGCAGA ACGTTCCCAG 
CTCTTGGGTG AGAAAGAGCT GCTGATGTCA ACTGTGGAGG AACATGGGGA GAGCCAGAGC 
ATCCAAGAGC TCAGATCTTC TCAGGCGCAG TTAATAGAAG AGAGGGATAA CCTACGTACC 
CATTGCCAGA ACCTGCAGGT TTCACTGGAA CAAGCACATG AACAGCTCGG CTCCCAGATC 
CAGGAGCAGC AGCAGCTCAC TCTGTATTGG AAGGAAAGGT GGCAGCAGAC TGCTGTTAAT 
TTAAAAAGCA CAGAAGAACA ACTGGAACAG GCAAAGGCTC TTTGCTCTAG TACAACAAAT 
AAGCACTCTG AGATCCTCCA AGATTATGAA CTCCTACAAA CAGATGCAGA GGAGCTGGCT 
GAACTCAGGA TGGCTATCAG CAGCCTGAAG AAAGAAAACA GCCTTTTAAA AGAGCAGATA 
AAGGAGCAGG AACAGAACAA GCATCTGCAG TGTCTTCAGA GCCACATCCA GGCTAATGTG 
GACAGCCCAG AGGATATGAA TTCGAAACTT GGCAAGCTGT ATAAAGAATT ACAGCAGGCT 
CACGAAAGGT TAAAATTCTT GGAGAGAGAA AGGTCTGAAA AAGAACTGGA AATAAAAAAA 
CTTAAGGCTG AGAGTGGATC CATACTTCGC ATTGAGCTAG ATGCCTGCAA ACAACAGTTG 
GAATTGGAGA GAAGTCGAAC AGCGACTCTA CAGGCCAAGA TTTCAGTCAT GGATTCCAGA 
AACAAAATGG AGGTTGCAAG TAGTCAGGAT CGTGTAGACA GTTTGCAGAA CCACAGCTTG 
TCCCTTCTAT CAGAAAGGTT AACATCAGAC GAATTGAGTT CTGAGATTGA GAATCTTCGT 
CAGCAGCTAG AAGAGGAGAG AGATGCAAAA AAGAAGAAAG AAGAACTGAT ATTTAATCTA 
AAAGAAGAGC TTGATGATCT CAAGCACAAG AAGCCTGGAG AAATCAAGGC ATCTCTGGAG 
GAAGTGGACT CAGAGCTAAT GTTTGTGCGA GAAGAACTGC AGAAAGTCTG GGAAATGTTG 
AAGACAAAAG ATACAGAACT TGAGGAACAG TATCAGGAGC TGGAGTCTGC TCGAGGCCAG 
TACACAGAGT GCAGCACAGA AAAAATGCGA CTGGAGCAAG TTCTTGCCAC CTTGCAGCAG 
CAGTTGACTG AGAAAGAGCA GACAATGAAA CATCTCAAGC AAATGAGAGA AATGGAGAAA 
ACTGAAATGG ATATTGAGAG GTCCTCATTG CAACTGAAGC TGGCAGAGCT ACAAGGACAA 
AAAGGAGATG TCACAACTCA GGCTGGGCAG CCGATAGAGG AGACTGGGCC TAAATGGTGG 
CGTTCAAAGG GAATGAAGGT TGTAGAGTCA TCAAAGAAAT GTATTCGCTG TGAAGCATTT 
TTGCAACAGT TGGATGAGAC AATCAAGGGT TGCCAGGAAC ACAATGCCGA GTTACAAGAA 
GAGAAGAACC AAACCTTGGC ATCCCTATAC CAGTTACAAG AAGTCCTAAC AAACCTCTCA 
AAACAATCAA AAGTAAATGA ACACGTGGCC CAGAGTCTAC AGGCGGACAA TGACTCTCTG 
AAAAAACAAC ACAGACTGGT GACAGAACAG CTTAAATGTC TGTTCAAAGA GAAAGAATCT 
CTGGGAAAAG CCTACCATAA GCTTCCAAAG AAGGATAAGC CAACGGAGGA CTGGAATGTT 
CAGTCCAGAT TAGTAAAGAA TATGCTGAGT TCTATAAAAA GCCATAAAAA CCAGCAAGAA 
GAGCTAACAG AAGAGGCAGG CCATCAACCA ATGGAATTCG ATGCTGTGCA GATGGAAACT 
AATGAAAGAG ACATGAGGAA CTTGCAAAGA CAGCTTGAGG AAAAGTCTGA GCAGATATTG 
AAAATGGCTT CAGAAATGAA ACTGTTACAA GAAAAAAATG AGTCTTTAAT GAAAGCTAAG 
ATGCGTTTTC AACAACAAGT CCAGCAAATT CGGAGCATGC CACATCTAAG TCATCAAAGA 
GAAAAGACAG ATCCTACTGT ACCACCTCTG TCTGGAGACA TCAACACCTC CTTTCACAAA 
AAGAATGAGT TTTTGGCAGC AGAGAGCCAA GATTGTTCTG GTAGAATGCA GGGAACACCA 
ACTTCATTTC AGAGTGCCTC AGAGGGGTCC TGGACATCAT CACCTCAAAA CTCAAGACCT 
CCAACACCCA TCAACCAAAA GGTTATGCCA TCTGCATCTT TACTGAGAGT TCAGCTTCCT 
GGCACAGTGG GGAAACACTG GGTAAAATCA AAAGTGGAAT CTCCTTTAAG CCCCAGAAGC 
CTAGCAGACA GTGAAGGTAA CAGTGATACT CCAGGGTCAG TTACTCCAAG GACTTCTGCA 
TTGCTGTCTC CCAGACCTTT CCATTCACCA AAATCGGCAC AGGCCTTCAG ATATGGAAAG 
AATGTGCAAC ACACAAACAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100496327 ( XM_012966921.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822375.2 Xenopus tropicalis rho-associated protein kinase 1 (LOC100496327), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa55017
Clone ID Related Accession (Same CDS sequence)	XM_012966921.2
Accession Version	XM_012966921.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	myosin-11 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966921.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTATA GAGTGTGTAA CGGTTATGTG AGTTTGCCCT CCCGGTATGG AACAACTGGG 
AACTCTCAGT ATCATGTTGA CAGCGGCAGG ATATCTCCTT GCTGGTCCTA CAAAGATCTT 
GGAAGCAGCA GACATGGTTT GGAGTTATCC AAATCCCCCG TTTTATCTGA CCTAGAGAAA 
AATATTGAGA CAGCGGTGAA TTTAGAATCT AGCTTTCTGC CTGGGCAATG GAAAGATTAC 
AGCAGGAGTT CAGTAAGTCC ACATCATTCA GAGAGAGCGA GGTCTACAAG TCCAATGCGG 
GGGATCCACT CTCTAGAATG TTCCAGAATC CAGTCCACTC CTCCCATTTC AAGAAGAAAT 
TCACTTTCCC GTCTACCAAC TTCATCTTCT GAGGATTTCA GTCTTGAGCT GCCATTGAGC 
CGCAGAGGGT GGAGTGGGTC AGAGTTGGAT CTAAAGGCAT CATTGACTGA AAGTGCCCAA 
AAGCGGGCTG ATCTGGTGCA GAGACTGAAA GATGCTCAGG TGAAGCTGGA GGGCCAATCA 
GAGGAGCTGC GGCACCGGGA TAAGGAGCTG GAGTTCAGCA GAGCTAAAAT TGAATTACTG 
GCAGTGAAGC AAAAGCAACT GGAAACATCA ATATCTCAGT TAGAGAAGGA AAAGGGTTGG 
TTAGAGGTGT CCCGCTATGA GGATAAGAGG CAACGTGGTG AACTGCAAGA CCGGATTATT 
AATCTAGAAA TGGAAGTGAT GAAGACCAAA TCTAACCTGG AAAATCTTAA CTACACCAGT 
GTACTAAAGT ATCAGAAGAC TCCAATGGAA AATGATGAAC TAAGTCGAGA ACTAAGAACT 
GCACGTGAGA ATCTACTCTA CCTTCGAAAT CGAGTCAAAG TCCTGGACGC AGAGAAGACT 
CAATCTGTTG AGGAACTAAG GATATTGAGA GAGGGGAGTC AGCAGGCCTT TAGCCAAACC 
AATGATGCAA ACCAGAGGGT AACAGACACA CTCCGGGCTC AAAAAGATCT GCAAGAAGAG 
CTGGCAAATC TGCAACTGAA CCTAAACACT ACCAGTCTGG AGAAGGAGCT CGTGTCCTCA 
AAGATTATGA GATTGGAGGA AAAGGTTAAA GATCTGAATT TAAGGCTAAA TATTGCACAT 
TCTGACAGGG AACGGGTTCT GCAGGACAAG GTGGAGCTGA CTAGGCGAAA TCAGGAATTG 
TCTCTCGAAC TAGAACGGGC TCAGAGGGGT CAGGAGGGGT TTAATGACCA GGTCAGTGAC 
CTTCACATTG AGCTGGTAGG AGCTAAAGCC CAGTCAAACC GTCAGGACCA GGAGAAGGTT 
CAGATGAAGG AGGAGCTGGT GATGCTGAAA CAGGTGAATG AGAAACTGAC TGCTGAACTG 
GGACAGACAC AGCATACACT CCAGCGCACC CTGGATGAAC TGCATCAGCT CCAGGCAGAA 
CACAAGATTT CAACCAACCT TACAGCTGCC TTGGAGGCAG AACGTTCCCA GCTCTTGGGT 
GAGAAAGAGC TGCTGATGTC AACTGTGGAG GAACATGGGG AGAGCCAGAG CATCCAAGAG 
CTCAGATCTT CTCAGGCGCA GTTAATAGAA GAGAGGGATA ACCTACGTAC CCATTGCCAG 
AACCTGCAGG TTTCACTGGA ACAAGCACAT GAACAGCTCG GCTCCCAGAT CCAGGAGCAG 
CAGCAGCTCA CTCTGTATTG GAAGGAAAGG TGGCAGCAGA CTGCTGTTAA TTTAAAAAGC 
ACAGAAGAAC AACTGGAACA GGCAAAGGCT CTTTGCTCTA GTACAACAAA TAAGCACTCT 
GAGATCCTCC AAGATTATGA ACTCCTACAA ACAGATGCAG AGGAGCTGGC TGAACTCAGG 
ATGGCTATCA GCAGCCTGAA GAAAGAAAAC AGCCTTTTAA AAGAGCAGAT AAAGGAGCAG 
GAACAGAACA AGCATCTGCA GTGTCTTCAG AGCCACATCC AGGCTAATGT GGACAGCCCA 
GAGGATATGA ATTCGAAACT TGGCAAGCTG TATAAAGAAT TACAGCAGGC TCACGAAAGG 
TTAAAATTCT TGGAGAGAGA AAGGTCTGAA AAAGAACTGG AAATAAAAAA ACTTAAGGCT 
GAGAGTGGAT CCATACTTCG CATTGAGCTA GATGCCTGCA AACAACAGTT GGAATTGGAG 
AGAAGTCGAA CAGCGACTCT ACAGGCCAAG ATTTCAGTCA TGGATTCCAG AAACAAAATG 
GAGGTTGCAA GTAGTCAGGA TCGTGTAGAC AGTTTGCAGA ACCACAGCTT GTCCCTTCTA 
TCAGAAAGGT TAACATCAGA CGAATTGAGT TCTGAGATTG AGAATCTTCG TCAGCAGCTA 
GAAGAGGAGA GAGATGCAAA AAAGAAGAAA GAAGAACTGA TATTTAATCT AAAAGAAGAG 
CTTGATGATC TCAAGCACAA GAAGCCTGGA GAAATCAAGG CATCTCTGGA GGAAGTGGAC 
TCAGAGCTAA TGTTTGTGCG AGAAGAACTG CAGAAAGTCT GGGAAATGTT GAAGACAAAA 
GATACAGAAC TTGAGGAACA GTATCAGGAG CTGGAGTCTG CTCGAGGCCA GTACACAGAG 
TGCAGCACAG AAAAAATGCG ACTGGAGCAA GTTCTTGCCA CCTTGCAGCA GCAGTTGACT 
GAGAAAGAGC AGACAATGAA ACATCTCAAG CAAATGAGAG AAATGGAGAA AACTGAAATG 
GATATTGAGA GGTCCTCATT GCAACTGAAG CTGGCAGAGC TACAAGGACA AAAAGGAGAT 
GTCACAACTC AGGCTGGGCA GCCGATAGAG GAGACTGGGC CTAAATGGTG GCGTTCAAAG 
GGAATGAAGG TTGTAGAGTC ATCAAAGAAA TGTATTCGCT GTGAAGCATT TTTGCAACAG 
TTGGATGAGA CAATCAAGGG TTGCCAGGAA CACAATGCCG AGTTACAAGA AGAGAAGAAC 
CAAACCTTGG CATCCCTATA CCAGTTACAA GAAGTCCTAA CAAACCTCTC AAAACAATCA 
AAAGTAAATG AACACGTGGC CCAGAGTCTA CAGGCGGACA ATGACTCTCT GAAAAAACAA 
CACAGACTGG TGACAGAACA GCTTAAATGT CTGTTCAAAG AGAAAGAATC TCTGGGAAAA 
GCCTACCATA AGCTTCCAAA GAAGGATAAG CCAACGGAGG ACTGGAATGT TCAGTCCAGA 
TTAGTAAAGA ATATGCTGAG TTCTATAAAA AGCCATAAAA ACCAGCAAGA AGAGCTAACA 
GAAGAGGCAG GCCATCAACC AATGGAATTC GATGCTGTGC AGATGGAAAC TAATGAAAGA 
GACATGAGGA ACTTGCAAAG ACAGCTTGAG GAAAAGTCTG AGCAGATATT GAAAATGGCT 
TCAGAAATGA AACTGTTACA AGAAAAAAAT GAGTCTTTAA TGAAAGCTAA GATGCGTTTT 
CAACAACAAG TCCAGCAAAT TCGGAGCATG CCACATCTAA GTCATCAAAG AGAAAAGACA 
GATCCTACTG TACCACCTCT GTCTGGAGAC ATCAACACCT CCTTTCACAA AAAGAATGAG 
TTTTTGGCAG CAGAGAGCCA AGATTGTTCT GGTAGAATGC AGGGAACACC AACTTCATTT 
CAGAGTGCCT CAGAGGGGTC CTGGACATCA TCACCTCAAA ACTCAAGACC TCCAACACCC 
ATCAACCAAA AGGTTATGCC ATCTGCATCT TTACTGAGAG TTCAGCTTCC TGGCACAGTG 
GGGAAACACT GGGTAAAATC AAAAGTGGAA TCTCCTTTAA GCCCCAGAAG CCTAGCAGAC 
AGTGAAGGTA ACAGTGATAC TCCAGGGTCA GTTACTCCAA GGACTTCTGC ATTGCTGTCT 
CCCAGACCTT TCCATTCACC AAAATCGGCA CAGGCCTTCA GATATGGAAA GAATGTGCAA 
CACACAAACA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822375.2
CDS	285..4199
Translation	MSYRVCNGYVSLPSRYGTTGNSQYHVDSGRISPCWSYKDLGSSRHGLELSKSPVLSDLEKNIETAVNLESSFLPGQWKDYSRSSVSPHHSERARSTSPMRGIHSLECSRIQSTPPISRRNSLSRLPTSSSEDFSLELPLSRRGWSGSELDLKASLTESAQKRADLVQRLKDAQVKLEGQSEELRHRDKELEFSRAKIELLAVKQKQLETSISQLEKEKGWLEVSRYEDKRQRGELQDRIINLEMEVMKTKSNLENLNYTSVLKYQKTPMENDELSRELRTARENLLYLRNRVKVLDAEKTQSVEELRILREGSQQAFSQTNDANQRVTDTLRAQKDLQEELANLQLNLNTTSLEKELVSSKIMRLEEKVKDLNLRLNIAHSDRERVLQDKVELTRRNQELSLELERAQRGQEGFNDQVSDLHIELVGAKAQSNRQDQEKVQMKEELVMLKQVNEKLTAELGQTQHTLQRTLDELHQLQAEHKISTNLTAALEAERSQLLGEKELLMSTVEEHGESQSIQELRSSQAQLIEERDNLRTHCQNLQVSLEQAHEQLGSQIQEQQQLTLYWKERWQQTAVNLKSTEEQLEQAKALCSSTTNKHSEILQDYELLQTDAEELAELRMAISSLKKENSLLKEQIKEQEQNKHLQCLQSHIQANVDSPEDMNSKLGKLYKELQQAHERLKFLERERSEKELEIKKLKAESGSILRIELDACKQQLELERSRTATLQAKISVMDSRNKMEVASSQDRVDSLQNHSLSLLSERLTSDELSSEIENLRQQLEEERDAKKKKEELIFNLKEELDDLKHKKPGEIKASLEEVDSELMFVREELQKVWEMLKTKDTELEEQYQELESARGQYTECSTEKMRLEQVLATLQQQLTEKEQTMKHLKQMREMEKTEMDIERSSLQLKLAELQGQKGDVTTQAGQPIEETGPKWWRSKGMKVVESSKKCIRCEAFLQQLDETIKGCQEHNAELQEEKNQTLASLYQLQEVLTNLSKQSKVNEHVAQSLQADNDSLKKQHRLVTEQLKCLFKEKESLGKAYHKLPKKDKPTEDWNVQSRLVKNMLSSIKSHKNQQEELTEEAGHQPMEFDAVQMETNERDMRNLQRQLEEKSEQILKMASEMKLLQEKNESLMKAKMRFQQQVQQIRSMPHLSHQREKTDPTVPPLSGDINTSFHKKNEFLAAESQDCSGRMQGTPTSFQSASEGSWTSSPQNSRPPTPINQKVMPSASLLRVQLPGTVGKHWVKSKVESPLSPRSLADSEGNSDTPGSVTPRTSALLSPRPFHSPKSAQAFRYGKNVQHTNS

Target ORF information:

RefSeq Version	XM_012966921.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis rho-associated protein kinase 1 (LOC100496327), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966921.2

ORF Insert Sequence:

ATGTCTTATA GAGTGTGTAA CGGTTATGTG AGTTTGCCCT CCCGGTATGG AACAACTGGG 
AACTCTCAGT ATCATGTTGA CAGCGGCAGG ATATCTCCTT GCTGGTCCTA CAAAGATCTT 
GGAAGCAGCA GACATGGTTT GGAGTTATCC AAATCCCCCG TTTTATCTGA CCTAGAGAAA 
AATATTGAGA CAGCGGTGAA TTTAGAATCT AGCTTTCTGC CTGGGCAATG GAAAGATTAC 
AGCAGGAGTT CAGTAAGTCC ACATCATTCA GAGAGAGCGA GGTCTACAAG TCCAATGCGG 
GGGATCCACT CTCTAGAATG TTCCAGAATC CAGTCCACTC CTCCCATTTC AAGAAGAAAT 
TCACTTTCCC GTCTACCAAC TTCATCTTCT GAGGATTTCA GTCTTGAGCT GCCATTGAGC 
CGCAGAGGGT GGAGTGGGTC AGAGTTGGAT CTAAAGGCAT CATTGACTGA AAGTGCCCAA 
AAGCGGGCTG ATCTGGTGCA GAGACTGAAA GATGCTCAGG TGAAGCTGGA GGGCCAATCA 
GAGGAGCTGC GGCACCGGGA TAAGGAGCTG GAGTTCAGCA GAGCTAAAAT TGAATTACTG 
GCAGTGAAGC AAAAGCAACT GGAAACATCA ATATCTCAGT TAGAGAAGGA AAAGGGTTGG 
TTAGAGGTGT CCCGCTATGA GGATAAGAGG CAACGTGGTG AACTGCAAGA CCGGATTATT 
AATCTAGAAA TGGAAGTGAT GAAGACCAAA TCTAACCTGG AAAATCTTAA CTACACCAGT 
GTACTAAAGT ATCAGAAGAC TCCAATGGAA AATGATGAAC TAAGTCGAGA ACTAAGAACT 
GCACGTGAGA ATCTACTCTA CCTTCGAAAT CGAGTCAAAG TCCTGGACGC AGAGAAGACT 
CAATCTGTTG AGGAACTAAG GATATTGAGA GAGGGGAGTC AGCAGGCCTT TAGCCAAACC 
AATGATGCAA ACCAGAGGGT AACAGACACA CTCCGGGCTC AAAAAGATCT GCAAGAAGAG 
CTGGCAAATC TGCAACTGAA CCTAAACACT ACCAGTCTGG AGAAGGAGCT CGTGTCCTCA 
AAGATTATGA GATTGGAGGA AAAGGTTAAA GATCTGAATT TAAGGCTAAA TATTGCACAT 
TCTGACAGGG AACGGGTTCT GCAGGACAAG GTGGAGCTGA CTAGGCGAAA TCAGGAATTG 
TCTCTCGAAC TAGAACGGGC TCAGAGGGGT CAGGAGGGGT TTAATGACCA GGTCAGTGAC 
CTTCACATTG AGCTGGTAGG AGCTAAAGCC CAGTCAAACC GTCAGGACCA GGAGAAGGTT 
CAGATGAAGG AGGAGCTGGT GATGCTGAAA CAGGTGAATG AGAAACTGAC TGCTGAACTG 
GGACAGACAC AGCATACACT CCAGCGCACC CTGGATGAAC TGCATCAGCT CCAGGCAGAA 
CACAAGATTT CAACCAACCT TACAGCTGCC TTGGAGGCAG AACGTTCCCA GCTCTTGGGT 
GAGAAAGAGC TGCTGATGTC AACTGTGGAG GAACATGGGG AGAGCCAGAG CATCCAAGAG 
CTCAGATCTT CTCAGGCGCA GTTAATAGAA GAGAGGGATA ACCTACGTAC CCATTGCCAG 
AACCTGCAGG TTTCACTGGA ACAAGCACAT GAACAGCTCG GCTCCCAGAT CCAGGAGCAG 
CAGCAGCTCA CTCTGTATTG GAAGGAAAGG TGGCAGCAGA CTGCTGTTAA TTTAAAAAGC 
ACAGAAGAAC AACTGGAACA GGCAAAGGCT CTTTGCTCTA GTACAACAAA TAAGCACTCT 
GAGATCCTCC AAGATTATGA ACTCCTACAA ACAGATGCAG AGGAGCTGGC TGAACTCAGG 
ATGGCTATCA GCAGCCTGAA GAAAGAAAAC AGCCTTTTAA AAGAGCAGAT AAAGGAGCAG 
GAACAGAACA AGCATCTGCA GTGTCTTCAG AGCCACATCC AGGCTAATGT GGACAGCCCA 
GAGGATATGA ATTCGAAACT TGGCAAGCTG TATAAAGAAT TACAGCAGGC TCACGAAAGG 
TTAAAATTCT TGGAGAGAGA AAGGTCTGAA AAAGAACTGG AAATAAAAAA ACTTAAGGCT 
GAGAGTGGAT CCATACTTCG CATTGAGCTA GATGCCTGCA AACAACAGTT GGAATTGGAG 
AGAAGTCGAA CAGCGACTCT ACAGGCCAAG ATTTCAGTCA TGGATTCCAG AAACAAAATG 
GAGGTTGCAA GTAGTCAGGA TCGTGTAGAC AGTTTGCAGA ACCACAGCTT GTCCCTTCTA 
TCAGAAAGGT TAACATCAGA CGAATTGAGT TCTGAGATTG AGAATCTTCG TCAGCAGCTA 
GAAGAGGAGA GAGATGCAAA AAAGAAGAAA GAAGAACTGA TATTTAATCT AAAAGAAGAG 
CTTGATGATC TCAAGCACAA GAAGCCTGGA GAAATCAAGG CATCTCTGGA GGAAGTGGAC 
TCAGAGCTAA TGTTTGTGCG AGAAGAACTG CAGAAAGTCT GGGAAATGTT GAAGACAAAA 
GATACAGAAC TTGAGGAACA GTATCAGGAG CTGGAGTCTG CTCGAGGCCA GTACACAGAG 
TGCAGCACAG AAAAAATGCG ACTGGAGCAA GTTCTTGCCA CCTTGCAGCA GCAGTTGACT 
GAGAAAGAGC AGACAATGAA ACATCTCAAG CAAATGAGAG AAATGGAGAA AACTGAAATG 
GATATTGAGA GGTCCTCATT GCAACTGAAG CTGGCAGAGC TACAAGGACA AAAAGGAGAT 
GTCACAACTC AGGCTGGGCA GCCGATAGAG GAGACTGGGC CTAAATGGTG GCGTTCAAAG 
GGAATGAAGG TTGTAGAGTC ATCAAAGAAA TGTATTCGCT GTGAAGCATT TTTGCAACAG 
TTGGATGAGA CAATCAAGGG TTGCCAGGAA CACAATGCCG AGTTACAAGA AGAGAAGAAC 
CAAACCTTGG CATCCCTATA CCAGTTACAA GAAGTCCTAA CAAACCTCTC AAAACAATCA 
AAAGTAAATG AACACGTGGC CCAGAGTCTA CAGGCGGACA ATGACTCTCT GAAAAAACAA 
CACAGACTGG TGACAGAACA GCTTAAATGT CTGTTCAAAG AGAAAGAATC TCTGGGAAAA 
GCCTACCATA AGCTTCCAAA GAAGGATAAG CCAACGGAGG ACTGGAATGT TCAGTCCAGA 
TTAGTAAAGA ATATGCTGAG TTCTATAAAA AGCCATAAAA ACCAGCAAGA AGAGCTAACA 
GAAGAGGCAG GCCATCAACC AATGGAATTC GATGCTGTGC AGATGGAAAC TAATGAAAGA 
GACATGAGGA ACTTGCAAAG ACAGCTTGAG GAAAAGTCTG AGCAGATATT GAAAATGGCT 
TCAGAAATGA AACTGTTACA AGAAAAAAAT GAGTCTTTAA TGAAAGCTAA GATGCGTTTT 
CAACAACAAG TCCAGCAAAT TCGGAGCATG CCACATCTAA GTCATCAAAG AGAAAAGACA 
GATCCTACTG TACCACCTCT GTCTGGAGAC ATCAACACCT CCTTTCACAA AAAGAATGAG 
TTTTTGGCAG CAGAGAGCCA AGATTGTTCT GGTAGAATGC AGGGAACACC AACTTCATTT 
CAGAGTGCCT CAGAGGGGTC CTGGACATCA TCACCTCAAA ACTCAAGACC TCCAACACCC 
ATCAACCAAA AGGTTATGCC ATCTGCATCT TTACTGAGAG TTCAGCTTCC TGGCACAGTG 
GGGAAACACT GGGTAAAATC AAAAGTGGAA TCTCCTTTAA GCCCCAGAAG CCTAGCAGAC 
AGTGAAGGTA ACAGTGATAC TCCAGGGTCA GTTACTCCAA GGACTTCTGC ATTGCTGTCT 
CCCAGACCTT TCCATTCACC AAAATCGGCA CAGGCCTTCA GATATGGAAA GAATGTGCAA 
CACACAAACA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100496327 ( XM_031899137.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754997.1 Xenopus tropicalis rho-associated protein kinase 1 (LOC100496327), transcript variant X3, mRNA.

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CloneID	OXa55018
Clone ID Related Accession (Same CDS sequence)	XM_031899137.1
Accession Version	XM_031899137.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho-associated protein kinase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTATA GAGTGTGTAA CGGTTATGTG AGTTTGCCCT CCCGGTATGG AACAACTGGG 
AACTCTCAGT ATCATGTTGA CAGCGGCAGG ATATCTCCTT GCTGGTCCTA CAAAGATCTT 
GGAAGCAGCA GACATGGTTT GGAGTTATCC AAATCCCCCG TTTTATCTGA CCTAGAGAAA 
AATATTGAGA CAGCGGTGAA TTTAGAATCT AGCTTTCTGC CTGGGCAATG GAAAGATTAC 
AGCAGGAGTT CAGTAAGTCC ACATCATTCA GAGAGAGCGA GGTCTACAAG TCCAATGCGG 
GGGATCCACT CTCTAGAATG TTCCAGAATC CAGTCCACTC CTCCCATTTC AAGAAGAAAT 
TCACTTTCCC GTCTACCAAC TTCATCTTCT GAGGATTTCA GTCTTGAGCT GCCATTGAGC 
CGCAGAGGGT GGAGTGGGTC AGAGTTGGAT CTAAAGGCAT CATTGACTGA AAGTGCCCAA 
AAGCGGGCTG ATCTGGTGCA GAGACTGAAA GATGCTCAGG TGAAGCTGGA GGGCCAATCA 
GAGGAGCTGC GGCACCGGGA TAAGGAGCTG GAGTTCAGCA GAGCTAAAAT TGAATTACTG 
GCAGTGAAGC AAAAGCAACT GGAAACATCA ATATCTCAGT TAGAGAAGGA AAAGGGTTGG 
TTAGAGGTGT CCCGCTATGA GGATAAGAGG CAACGTGGTG AACTGCAAGA CCGGATTATT 
AATCTAGAAA TGGAAGTGAT GAAGACCAAA TCTAACCTGG AAAATCTTAA CTACACCAGT 
GTACTAAAGT ATCAGAAGAC TCCAATGGAC TTAAAGGAAA ATGATGAACT AAGTCGAGAA 
CTAAGAACTG CACGTGAGAA TCTACTCTAC CTTCGAAATC GAGTCAAAGT CCTGGACGCA 
GAGAAGACTC AATCTGTTGA GGAACTAAGG ATATTGAGAG AGGGGAGTCA GCAGGCCTTT 
AGCCAAACCA ATGATGCAAA CCAGAGGGTA ACAGACACAC TCCGGGCTCA AAAAGATCTG 
CAAGAAGAGC TGGCAAATCT GCAACTGAAC CTAAACACTA CCAGTCTGGA CAAGGTGGAG 
CTGACTAGGC GAAATCAGGA ATTGTCTCTC GAACTAGAAC GGGCTCAGAG GGGTCAGGAG 
GGGTTTAATG ACCAGGTCAG TGACCTTCAC ATTGAGCTGG TAGGAGCTAA AGCCCAGTCA 
AACCGTCAGG ACCAGGAGAA GGTTCAGATG AAGGAGGAGC TGGTGATGCT GAAACAGGTG 
AATGAGAAAC TGACTGCTGA ACTGGGACAG ACACAGCATA CACTCCAGCG CACCCTGGAT 
GAACTGCATC AGCTCCAGGC AGAACACAAG ATTTCAACCA ACCTTACAGC TGCCTTGGAG 
GCAGAACGTT CCCAGCTCTT GGGTGAGAAA GAGCTGCTGA TGTCAACTGT GGAGGAACAT 
GGGGAGAGCC AGAGCATCCA AGAGCTCAGA TCTTCTCAGG CGCAGTTAAT AGAAGAGAGG 
GATAACCTAC GTACCCATTG CCAGAACCTG CAGGTTTCAC TGGAACAAGC ACATGAACAG 
CTCGGCTCCC AGATCCAGGA GCAGCAGCAG CTCACTCTGT ATTGGAAGGA AAGGTGGCAG 
CAGACTGCTG TTAATTTAAA AAGCACAGAA GAACAACTGG AACAGGCAAA GGCTCTTTGC 
TCTAGTACAA CAAATAAGCA CTCTGAGATC CTCCAAGATT ATGAACTCCT ACAAACAGAT 
GCAGAGGAGC TGGCTGAACT CAGGATGGCT ATCAGCAGCC TGAAGAAAGA AAACAGCCTT 
TTAAAAGAGC AGATAAAGGA GCAGGAACAG AACAAGCATC TGCAGTGTCT TCAGAGCCAC 
ATCCAGGCTA ATGTGGACAG CCCAGAGGAT ATGAATTCGA AACTTGGCAA GCTGTATAAA 
GAATTACAGC AGGCTCACGA AAGGTTAAAA TTCTTGGAGA GAGAAAGGTC TGAAAAAGAA 
CTGGAAATAA AAAAACTTAA GGCTGAGAGT GGATCCATAC TTCGCATTGA GCTAGATGCC 
TGCAAACAAC AGTTGGAATT GGAGAGAAGT CGAACAGCGA CTCTACAGGC CAAGATTTCA 
GTCATGGATT CCAGAAACAA AATGGAGGTT GCAAGTAGTC AGGATCGTGT AGACAGTTTG 
CAGAACCACA GCTTGTCCCT TCTATCAGAA AGGTTAACAT CAGACGAATT GAGTTCTGAG 
ATTGAGAATC TTCGTCAGCA GCTAGAAGAG GAGAGAGATG CAAAAAAGAA GAAAGAAGAA 
CTGATATTTA ATCTAAAAGA AGAGCTTGAT GATCTCAAGC ACAAGAAGCC TGGAGAAATC 
AAGGCATCTC TGGAGGAAGT GGACTCAGAG CTAATGTTTG TGCGAGAAGA ACTGCAGAAA 
GTCTGGGAAA TGTTGAAGAC AAAAGATACA GAACTTGAGG AACAGTATCA GGAGCTGGAG 
TCTGCTCGAG GCCAGTACAC AGAGTGCAGC ACAGAAAAAA TGCGACTGGA GCAAGTTCTT 
GCCACCTTGC AGCAGCAGTT GACTGAGAAA GAGCAGACAA TGAAACATCT CAAGCAAATG 
AGAGAAATGG AGAAAACTGA AATGGATATT GAGAGGTCCT CATTGCAACT GAAGCTGGCA 
GAGCTACAAG GACAAAAAGG AGATGTCACA ACTCAGGCTG GGCAGCCGAT AGAGGAGACT 
GGGCCTAAAT GGTGGCGTTC AAAGGGAATG AAGGTTGTAG AGTCATCAAA GAAATGTATT 
CGCTGTGAAG CATTTTTGCA ACAGTTGGAT GAGACAATCA AGGGTTGCCA GGAACACAAT 
GCCGAGTTAC AAGAAGAGAA GAACCAAACC TTGGCATCCC TATACCAGTT ACAAGAAGTC 
CTAACAAACC TCTCAAAACA ATCAAAAGTA AATGAACACG TGGCCCAGAG TCTACAGGCG 
GACAATGACT CTCTGAAAAA ACAACACAGA CTGGTGACAG AACAGCTTAA ATGTCTGTTC 
AAAGAGAAAG AATCTCTGGG AAAAGCCTAC CATAAGCTTC CAAAGAAGGA TAAGCCAACG 
GAGGACTGGA ATGTTCAGTC CAGATTAGTA AAGAATATGC TGAGTTCTAT AAAAAGCCAT 
AAAAACCAGC AAGAAGAGCT AACAGAAGAG GCAGGCCATC AACCAATGGA ATTCGATGCT 
GTGCAGATGG AAACTAATGA AAGAGACATG AGGAACTTGC AAAGACAGCT TGAGGAAAAG 
TCTGAGCAGA TATTGAAAAT GGCTTCAGAA ATGAAACTGT TACAAGAAAA AAATGAGTCT 
TTAATGAAAG CTAAGATGCG TTTTCAACAA CAAGTCCAGC AAATTCGGAG CATGCCACAT 
CTAAGTCATC AAAGAGAAAA GACAGATCCT ACTGTACCAC CTCTGTCTGG AGACATCAAC 
ACCTCCTTTC ACAAAAAGAA TGAGTTTTTG GCAGCAGAGA GCCAAGATTG TTCTGGTAGA 
ATGCAGGGAA CACCAACTTC ATTTCAGAGT GCCTCAGAGG GGTCCTGGAC ATCATCACCT 
CAAAACTCAA GACCTCCAAC ACCCATCAAC CAAAAGGTTA TGCCATCTGC ATCTTTACTG 
AGAGTTCAGC TTCCTGGCAC AGTGGGGAAA CACTGGGTAA AATCAAAAGT GGAATCTCCT 
TTAAGCCCCA GAAGCCTAGC AGACAGTGAA GGTAACAGTG ATACTCCAGG GTCAGTTACT 
CCAAGGACTT CTGCATTGCT GTCTCCCAGA CCTTTCCATT CACCAAAATC GGCACAGGCC 
TTCAGATATG GAAAGAATGT GCAACACACA AACAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754997.1
CDS	285..4103
Translation	MSYRVCNGYVSLPSRYGTTGNSQYHVDSGRISPCWSYKDLGSSRHGLELSKSPVLSDLEKNIETAVNLESSFLPGQWKDYSRSSVSPHHSERARSTSPMRGIHSLECSRIQSTPPISRRNSLSRLPTSSSEDFSLELPLSRRGWSGSELDLKASLTESAQKRADLVQRLKDAQVKLEGQSEELRHRDKELEFSRAKIELLAVKQKQLETSISQLEKEKGWLEVSRYEDKRQRGELQDRIINLEMEVMKTKSNLENLNYTSVLKYQKTPMDLKENDELSRELRTARENLLYLRNRVKVLDAEKTQSVEELRILREGSQQAFSQTNDANQRVTDTLRAQKDLQEELANLQLNLNTTSLDKVELTRRNQELSLELERAQRGQEGFNDQVSDLHIELVGAKAQSNRQDQEKVQMKEELVMLKQVNEKLTAELGQTQHTLQRTLDELHQLQAEHKISTNLTAALEAERSQLLGEKELLMSTVEEHGESQSIQELRSSQAQLIEERDNLRTHCQNLQVSLEQAHEQLGSQIQEQQQLTLYWKERWQQTAVNLKSTEEQLEQAKALCSSTTNKHSEILQDYELLQTDAEELAELRMAISSLKKENSLLKEQIKEQEQNKHLQCLQSHIQANVDSPEDMNSKLGKLYKELQQAHERLKFLERERSEKELEIKKLKAESGSILRIELDACKQQLELERSRTATLQAKISVMDSRNKMEVASSQDRVDSLQNHSLSLLSERLTSDELSSEIENLRQQLEEERDAKKKKEELIFNLKEELDDLKHKKPGEIKASLEEVDSELMFVREELQKVWEMLKTKDTELEEQYQELESARGQYTECSTEKMRLEQVLATLQQQLTEKEQTMKHLKQMREMEKTEMDIERSSLQLKLAELQGQKGDVTTQAGQPIEETGPKWWRSKGMKVVESSKKCIRCEAFLQQLDETIKGCQEHNAELQEEKNQTLASLYQLQEVLTNLSKQSKVNEHVAQSLQADNDSLKKQHRLVTEQLKCLFKEKESLGKAYHKLPKKDKPTEDWNVQSRLVKNMLSSIKSHKNQQEELTEEAGHQPMEFDAVQMETNERDMRNLQRQLEEKSEQILKMASEMKLLQEKNESLMKAKMRFQQQVQQIRSMPHLSHQREKTDPTVPPLSGDINTSFHKKNEFLAAESQDCSGRMQGTPTSFQSASEGSWTSSPQNSRPPTPINQKVMPSASLLRVQLPGTVGKHWVKSKVESPLSPRSLADSEGNSDTPGSVTPRTSALLSPRPFHSPKSAQAFRYGKNVQHTNS

Target ORF information:

RefSeq Version	XM_031899137.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis rho-associated protein kinase 1 (LOC100496327), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899137.1

ORF Insert Sequence:

ATGTCTTATA GAGTGTGTAA CGGTTATGTG AGTTTGCCCT CCCGGTATGG AACAACTGGG 
AACTCTCAGT ATCATGTTGA CAGCGGCAGG ATATCTCCTT GCTGGTCCTA CAAAGATCTT 
GGAAGCAGCA GACATGGTTT GGAGTTATCC AAATCCCCCG TTTTATCTGA CCTAGAGAAA 
AATATTGAGA CAGCGGTGAA TTTAGAATCT AGCTTTCTGC CTGGGCAATG GAAAGATTAC 
AGCAGGAGTT CAGTAAGTCC ACATCATTCA GAGAGAGCGA GGTCTACAAG TCCAATGCGG 
GGGATCCACT CTCTAGAATG TTCCAGAATC CAGTCCACTC CTCCCATTTC AAGAAGAAAT 
TCACTTTCCC GTCTACCAAC TTCATCTTCT GAGGATTTCA GTCTTGAGCT GCCATTGAGC 
CGCAGAGGGT GGAGTGGGTC AGAGTTGGAT CTAAAGGCAT CATTGACTGA AAGTGCCCAA 
AAGCGGGCTG ATCTGGTGCA GAGACTGAAA GATGCTCAGG TGAAGCTGGA GGGCCAATCA 
GAGGAGCTGC GGCACCGGGA TAAGGAGCTG GAGTTCAGCA GAGCTAAAAT TGAATTACTG 
GCAGTGAAGC AAAAGCAACT GGAAACATCA ATATCTCAGT TAGAGAAGGA AAAGGGTTGG 
TTAGAGGTGT CCCGCTATGA GGATAAGAGG CAACGTGGTG AACTGCAAGA CCGGATTATT 
AATCTAGAAA TGGAAGTGAT GAAGACCAAA TCTAACCTGG AAAATCTTAA CTACACCAGT 
GTACTAAAGT ATCAGAAGAC TCCAATGGAC TTAAAGGAAA ATGATGAACT AAGTCGAGAA 
CTAAGAACTG CACGTGAGAA TCTACTCTAC CTTCGAAATC GAGTCAAAGT CCTGGACGCA 
GAGAAGACTC AATCTGTTGA GGAACTAAGG ATATTGAGAG AGGGGAGTCA GCAGGCCTTT 
AGCCAAACCA ATGATGCAAA CCAGAGGGTA ACAGACACAC TCCGGGCTCA AAAAGATCTG 
CAAGAAGAGC TGGCAAATCT GCAACTGAAC CTAAACACTA CCAGTCTGGA CAAGGTGGAG 
CTGACTAGGC GAAATCAGGA ATTGTCTCTC GAACTAGAAC GGGCTCAGAG GGGTCAGGAG 
GGGTTTAATG ACCAGGTCAG TGACCTTCAC ATTGAGCTGG TAGGAGCTAA AGCCCAGTCA 
AACCGTCAGG ACCAGGAGAA GGTTCAGATG AAGGAGGAGC TGGTGATGCT GAAACAGGTG 
AATGAGAAAC TGACTGCTGA ACTGGGACAG ACACAGCATA CACTCCAGCG CACCCTGGAT 
GAACTGCATC AGCTCCAGGC AGAACACAAG ATTTCAACCA ACCTTACAGC TGCCTTGGAG 
GCAGAACGTT CCCAGCTCTT GGGTGAGAAA GAGCTGCTGA TGTCAACTGT GGAGGAACAT 
GGGGAGAGCC AGAGCATCCA AGAGCTCAGA TCTTCTCAGG CGCAGTTAAT AGAAGAGAGG 
GATAACCTAC GTACCCATTG CCAGAACCTG CAGGTTTCAC TGGAACAAGC ACATGAACAG 
CTCGGCTCCC AGATCCAGGA GCAGCAGCAG CTCACTCTGT ATTGGAAGGA AAGGTGGCAG 
CAGACTGCTG TTAATTTAAA AAGCACAGAA GAACAACTGG AACAGGCAAA GGCTCTTTGC 
TCTAGTACAA CAAATAAGCA CTCTGAGATC CTCCAAGATT ATGAACTCCT ACAAACAGAT 
GCAGAGGAGC TGGCTGAACT CAGGATGGCT ATCAGCAGCC TGAAGAAAGA AAACAGCCTT 
TTAAAAGAGC AGATAAAGGA GCAGGAACAG AACAAGCATC TGCAGTGTCT TCAGAGCCAC 
ATCCAGGCTA ATGTGGACAG CCCAGAGGAT ATGAATTCGA AACTTGGCAA GCTGTATAAA 
GAATTACAGC AGGCTCACGA AAGGTTAAAA TTCTTGGAGA GAGAAAGGTC TGAAAAAGAA 
CTGGAAATAA AAAAACTTAA GGCTGAGAGT GGATCCATAC TTCGCATTGA GCTAGATGCC 
TGCAAACAAC AGTTGGAATT GGAGAGAAGT CGAACAGCGA CTCTACAGGC CAAGATTTCA 
GTCATGGATT CCAGAAACAA AATGGAGGTT GCAAGTAGTC AGGATCGTGT AGACAGTTTG 
CAGAACCACA GCTTGTCCCT TCTATCAGAA AGGTTAACAT CAGACGAATT GAGTTCTGAG 
ATTGAGAATC TTCGTCAGCA GCTAGAAGAG GAGAGAGATG CAAAAAAGAA GAAAGAAGAA 
CTGATATTTA ATCTAAAAGA AGAGCTTGAT GATCTCAAGC ACAAGAAGCC TGGAGAAATC 
AAGGCATCTC TGGAGGAAGT GGACTCAGAG CTAATGTTTG TGCGAGAAGA ACTGCAGAAA 
GTCTGGGAAA TGTTGAAGAC AAAAGATACA GAACTTGAGG AACAGTATCA GGAGCTGGAG 
TCTGCTCGAG GCCAGTACAC AGAGTGCAGC ACAGAAAAAA TGCGACTGGA GCAAGTTCTT 
GCCACCTTGC AGCAGCAGTT GACTGAGAAA GAGCAGACAA TGAAACATCT CAAGCAAATG 
AGAGAAATGG AGAAAACTGA AATGGATATT GAGAGGTCCT CATTGCAACT GAAGCTGGCA 
GAGCTACAAG GACAAAAAGG AGATGTCACA ACTCAGGCTG GGCAGCCGAT AGAGGAGACT 
GGGCCTAAAT GGTGGCGTTC AAAGGGAATG AAGGTTGTAG AGTCATCAAA GAAATGTATT 
CGCTGTGAAG CATTTTTGCA ACAGTTGGAT GAGACAATCA AGGGTTGCCA GGAACACAAT 
GCCGAGTTAC AAGAAGAGAA GAACCAAACC TTGGCATCCC TATACCAGTT ACAAGAAGTC 
CTAACAAACC TCTCAAAACA ATCAAAAGTA AATGAACACG TGGCCCAGAG TCTACAGGCG 
GACAATGACT CTCTGAAAAA ACAACACAGA CTGGTGACAG AACAGCTTAA ATGTCTGTTC 
AAAGAGAAAG AATCTCTGGG AAAAGCCTAC CATAAGCTTC CAAAGAAGGA TAAGCCAACG 
GAGGACTGGA ATGTTCAGTC CAGATTAGTA AAGAATATGC TGAGTTCTAT AAAAAGCCAT 
AAAAACCAGC AAGAAGAGCT AACAGAAGAG GCAGGCCATC AACCAATGGA ATTCGATGCT 
GTGCAGATGG AAACTAATGA AAGAGACATG AGGAACTTGC AAAGACAGCT TGAGGAAAAG 
TCTGAGCAGA TATTGAAAAT GGCTTCAGAA ATGAAACTGT TACAAGAAAA AAATGAGTCT 
TTAATGAAAG CTAAGATGCG TTTTCAACAA CAAGTCCAGC AAATTCGGAG CATGCCACAT 
CTAAGTCATC AAAGAGAAAA GACAGATCCT ACTGTACCAC CTCTGTCTGG AGACATCAAC 
ACCTCCTTTC ACAAAAAGAA TGAGTTTTTG GCAGCAGAGA GCCAAGATTG TTCTGGTAGA 
ATGCAGGGAA CACCAACTTC ATTTCAGAGT GCCTCAGAGG GGTCCTGGAC ATCATCACCT 
CAAAACTCAA GACCTCCAAC ACCCATCAAC CAAAAGGTTA TGCCATCTGC ATCTTTACTG 
AGAGTTCAGC TTCCTGGCAC AGTGGGGAAA CACTGGGTAA AATCAAAAGT GGAATCTCCT 
TTAAGCCCCA GAAGCCTAGC AGACAGTGAA GGTAACAGTG ATACTCCAGG GTCAGTTACT 
CCAAGGACTT CTGCATTGCT GTCTCCCAGA CCTTTCCATT CACCAAAATC GGCACAGGCC 
TTCAGATATG GAAAGAATGT GCAACACACA AACAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100496327 ( XM_002938880.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002938926.2 Xenopus tropicalis myosin-11-like (LOC100496327), transcript variant X1, mRNA.

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CloneID	OXa13783
Clone ID Related Accession (Same CDS sequence)	XM_002938880.3
Accession Version	XM_002938880.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	myosin-11-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_002938880.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTATA GAGTGTGTAA CGGTTATGTG AGTTTGCCCT CCCGGTATGG AACAACTGGG 
AACTCTCAGT ATCATGTTGA CAGCGGCAGG ATATCTCCTT GCTGGTCCTA CAAAGATCTT 
GGCAGCAGCA GACATGGTTT GGAGTTATCC AAATCCCCCA TTTTATCTGA CCTAGAGAAA 
AATATTGAGA CAGCGGTGAA TTTAGAATCT AGCTTTCTGC CTGGGCAATG GAAAGATTAC 
AGCAGGAGTT CAGTAAGTCC ACATCATTCA GAGAGAGCGA GGTCTACAAG TCCAATGCGG 
GGGATCCACT CTCTAGAATG TTCCAGAATC CAGTCCACTC CTCCCATTTC AAGAAGAAAT 
TCACTTTCCC GTCTACCAAC TTCATCTTCT GAGGATTTCA GTCTTGAGCT GCCATTGAGC 
CGCAGAGGGT GGAGTGGGTC AGAGTTGGAT CTAAAGGCAT CATTGACTGA AAGTGCCCAA 
AAGCGGGCTG ATCTGGTGCA GAGACTGAAA GATGCTCAGG TGAAGCTGGA GGGCCAATCA 
GAGGAGCTGC GGCACCGGGA TAAGGAGCTG GAGTTCAGCA GAGCTAAAAT TGAATTACTG 
GCAGTGAAGC AAAAGCAACT GGAAACATCA ATATCTCAGT TAGAGAAGGA AAAGGGTTGG 
TTAGAGGTGT CCCGCTATGA GGATAAGAGG CAACGTGGTG AACTGCAAGA CCGGATTATT 
AATCTAGAAA TGGAAGTGAT GAAGACCAAA TCTAACCTGG AAAATCTTAA CTACACCAGT 
GTACTTAAGT ATCAGAAGAC TCCAATGGAC TTAAAGGAAA ATGATGAACT AAGTCGAGAA 
CTAAGAACTG CACGTGAGAA TCTACTCTAC CTTCGAAATC GAGTCAAAGT CCTGGACGCA 
GAGAAGACTC AATCTGTTGA GGAACTAAGG ATATTGAGAG AGGGGAGTCA GCAGGCCTTT 
AGCCAAACCA ATGATGCAAA CCAGAGGGTA ACAGACACAC TCCGGGCTCA AAAAGATCTG 
CAAGAAGAGC TGGCAAATCT GCAACTGAAA CTAAACACTA CCAGTCTGGA GAAGGAGCTC 
GTGTCCTCAA AGATTATGAG ATTGGAGGAA AAGGTTAAAG ATCTGAATTT AAGGCTAAAT 
ATTGCACATT CTGACAGGGA ACGGGTTCTG CAGGACAAGG TGGAGCTGAC TAGGCGAAAT 
CAGGAATTGT CTCTCGAACT AGAACGGGCT CAGAGGGGTC AGGAGGGGTT TAATGACCAG 
GTCAGTGACC TTCACATTGA GCTGGTAGGA GCTAAAGCCC AGTCAAACCG TCAGGACCAG 
GAGAAGGTTC AGATGAAGGA GGAGCTGGTG ATGCTGAAAC AGGTGAATGA GAAACTGACT 
GCTGAACTGG GACAGACACA GCATACACTC CAGCGCACCC TGGATGAACT GCATCAGCTC 
CAGGCAGAAC ACAAGATTTC AACCAACCTT ACAGCTGCCT TGGAGGCAGA ACGTTCCCAG 
CTCTTGGGTG AGAAAGAGCT GCTGATGTCA ACTGTGGAGG AACATGGGGA GAGCCAGAGC 
ATCCAAGAGC TCAGATCTTC TCAGGCGCAG TTAATAGAAG AGAGGGATAA CCTACGTACC 
CATTGCCAGA ACCTGCAGGT TTCACTGGAA CAAGCACATG AACAGCTCGG CTCCCAGATC 
CAGGAGCAGC AGCAGCTCAC TCTGTATTGG AAGGAAAGGT GGCAGCAGAC TGCTGTTAAT 
TTAAAAAGCA CAGAAGAACA ACTGGAACAG GCAAAGGCTC TTTGCTCTAG TACAACAAAT 
AAGCACTCTG AGATCCTCCA AGATTATGAA CTCCTACAAA CAGATGCAGA GGAGCTGGCT 
GAACTCAGGA TGGCTATCAG CAGCCTGAAG AAAGAAAACA GCCTTTTAAA AGAGCAGATA 
AAGGAGCAGG AACAGAACAA GCATCTGCAG TGTCTTCAGA GCCACATCCA GGCTAATGTG 
GACAGCCCAG AGGATATGAA TTCGAAACTT GGCAAGCTGT ATAAAGAATT ACAGCAGGCT 
CACGAAAGGT TAAAATTCTT GGAGAGAGAA AGGTCTGAAA AAGAACTGGA AATAAAAAAA 
CTTAAGGCTG AGAGTGGATC CATACTTCGC ATTGAGCTAG ATGCCTGCAA ACAACAGTTG 
GAATTGGAGA GAAGTCGAAC AGCGACTCTA CAGGCCAAGA TTTCAGTCAT GGATTCCAGA 
AACAAAATGG AGGTTGCAAG TAGTCAGGAT CGTGTAGACA GTTTGCAGAA CCACAGCTTG 
TCCCTTCTAT CAGAAAGGTT AACATCAGAC GAATTGAGTT CTGAGATTGA GAATCTTCGT 
CAGCAGCTAG AAGAGGAGAG AGATGCAAAA AAGAAGAAAG AAGAACTGAT ATTTAATCTA 
AAAGAAGAGC TTGATGATCT CAAGCACAAG AAGCCTGGAG AAATCAAGGC ATCTCTGGAG 
GAAGTGGACT CAGAGCTAAT GTTTGTGCGA GAAGAACTGC AGAAAGTCTG GGAAATGTTG 
AAGACAAAAG ATACAGAACT TGAGGAACAG TATCAGGAGC TGGAGTCTGC TCGAGGCCAG 
TACACAGAGT GCAGCACAGA AAAAATGCGA CTGGAGCAAG TTCTTGCCAC CTTGCAGCAG 
CAGTTGACTG AGAAAGAGCA GACAATGAAA CATCTCAAGC AAATGAGAGA AATGGAGAAA 
ACTGAAATGG ATATTGAGAG GTCCTCATTG CAACTGAAGC TGGCAGAGCT ACAAGGACAA 
AAAGGAGATG TCACAACTCA GGCTGGGCAG CCGATAGAGG AGACTGGGCC TAAATGGTGG 
CGTTCAAAGG GAATGAAGGT TGTAGAGTCA TCAAAGAAAT GTATTCGCTG TGAAGCATTT 
TTGCAACAGT TGGATGAGAC AATCAAGGGT TGCCAGGAAC ACAATGCCGA GTTACAAGAA 
GAGAAGAACC AAACCTTGGC ATCCCTATAC CAGTTACAAG AAGTCCTAAC AAACCTCTCA 
AAACAATCAA AAGTAAATGA ACACGTGGCC CAGAGTCTAC AGGCGGACAA TGACTCTCTG 
AAAAAACAAC ACAGACTGGT GACAGAACAG CTTAAATGTC TGTTCAAAGA GAAAGAATCT 
CTGGGAAAAG CCTACCATAA GCTTCCAAAG AAGGATAAGC CAACGGAGGA CTGGAATGTT 
CAGTCCAGAT TAGTAAAGAA TGTGCTGAGT TCTATAAAAA GCCATAAAAA CCAGCAAGAA 
GAGCTAACAG AAGAGGCAGG CCATCAACCA ATGGAATTCG ATGCTGTGCA GATGGAAACT 
AATGAAAGAG ACATGAGGAA CTTGCAAAGA CAGCTTGAGG AAAAGTCTGA GCAGATATTG 
AAAATGGCTT CAGAAATGAA ACTGTTACAA GAAAAAAATG AGTCTTTAAT GAAAGCTAAG 
ATGCGTTTTC AACAACAAGT CCAGCAAATT CGGAGCATGC CACATCTAAG TCATCAAAGA 
GAAAAGACAG ATCCTACTGT ACCACCTCTG TCTGGAGACA TCAACACCTC CTTTCACAAA 
AAGAATGAGT TTTTGGCAGC AGAGAGCCAA GATTGTTCTG GTAGAATGCA GGGAACACCA 
ACTTCATTTC AGAGTGCCTC AGAGGGGTCC TGGACATCAT CACCTCAAAA CTCAAGACCT 
CCAACACCCA TCAACCAAAA GGTTATGCCA TCTGCATCTT TACTGAGAGT TCAGCTTCCT 
GGCACAGTGG GGAAACACTG GGTAAAATCA AAAGTGGAAT CTCCTTTAAG CCCCAGAAGC 
CTAGCAGACA GTGAAGGTAA CAGTGATACT CCAGGGTCAG TTACTCCAAG GACTTCTGCA 
TTGCTGTCTC CCAGACCTTT CCATCCACCA AAATCGGCAC AGGCCTTCAG ATATGGAAAG 
AATGTGCAAC ACACAAACAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002938926.2
CDS	280..4203
Translation	MSYRVCNGYVSLPSRYGTTGNSQYHVDSGRISPCWSYKDLGSSRHGLELSKSPILSDLEKNIETAVNLESSFLPGQWKDYSRSSVSPHHSERARSTSPMRGIHSLECSRIQSTPPISRRNSLSRLPTSSSEDFSLELPLSRRGWSGSELDLKASLTESAQKRADLVQRLKDAQVKLEGQSEELRHRDKELEFSRAKIELLAVKQKQLETSISQLEKEKGWLEVSRYEDKRQRGELQDRIINLEMEVMKTKSNLENLNYTSVLKYQKTPMDLKENDELSRELRTARENLLYLRNRVKVLDAEKTQSVEELRILREGSQQAFSQTNDANQRVTDTLRAQKDLQEELANLQLKLNTTSLEKELVSSKIMRLEEKVKDLNLRLNIAHSDRERVLQDKVELTRRNQELSLELERAQRGQEGFNDQVSDLHIELVGAKAQSNRQDQEKVQMKEELVMLKQVNEKLTAELGQTQHTLQRTLDELHQLQAEHKISTNLTAALEAERSQLLGEKELLMSTVEEHGESQSIQELRSSQAQLIEERDNLRTHCQNLQVSLEQAHEQLGSQIQEQQQLTLYWKERWQQTAVNLKSTEEQLEQAKALCSSTTNKHSEILQDYELLQTDAEELAELRMAISSLKKENSLLKEQIKEQEQNKHLQCLQSHIQANVDSPEDMNSKLGKLYKELQQAHERLKFLERERSEKELEIKKLKAESGSILRIELDACKQQLELERSRTATLQAKISVMDSRNKMEVASSQDRVDSLQNHSLSLLSERLTSDELSSEIENLRQQLEEERDAKKKKEELIFNLKEELDDLKHKKPGEIKASLEEVDSELMFVREELQKVWEMLKTKDTELEEQYQELESARGQYTECSTEKMRLEQVLATLQQQLTEKEQTMKHLKQMREMEKTEMDIERSSLQLKLAELQGQKGDVTTQAGQPIEETGPKWWRSKGMKVVESSKKCIRCEAFLQQLDETIKGCQEHNAELQEEKNQTLASLYQLQEVLTNLSKQSKVNEHVAQSLQADNDSLKKQHRLVTEQLKCLFKEKESLGKAYHKLPKKDKPTEDWNVQSRLVKNVLSSIKSHKNQQEELTEEAGHQPMEFDAVQMETNERDMRNLQRQLEEKSEQILKMASEMKLLQEKNESLMKAKMRFQQQVQQIRSMPHLSHQREKTDPTVPPLSGDINTSFHKKNEFLAAESQDCSGRMQGTPTSFQSASEGSWTSSPQNSRPPTPINQKVMPSASLLRVQLPGTVGKHWVKSKVESPLSPRSLADSEGNSDTPGSVTPRTSALLSPRPFHPPKSAQAFRYGKNVQHTNS

Target ORF information:

RefSeq Version	XM_002938880.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin-11-like (LOC100496327), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002938880.3

ORF Insert Sequence:

ATGTCTTATA GAGTGTGTAA CGGTTATGTG AGTTTGCCCT CCCGGTATGG AACAACTGGG 
AACTCTCAGT ATCATGTTGA CAGCGGCAGG ATATCTCCTT GCTGGTCCTA CAAAGATCTT 
GGCAGCAGCA GACATGGTTT GGAGTTATCC AAATCCCCCA TTTTATCTGA CCTAGAGAAA 
AATATTGAGA CAGCGGTGAA TTTAGAATCT AGCTTTCTGC CTGGGCAATG GAAAGATTAC 
AGCAGGAGTT CAGTAAGTCC ACATCATTCA GAGAGAGCGA GGTCTACAAG TCCAATGCGG 
GGGATCCACT CTCTAGAATG TTCCAGAATC CAGTCCACTC CTCCCATTTC AAGAAGAAAT 
TCACTTTCCC GTCTACCAAC TTCATCTTCT GAGGATTTCA GTCTTGAGCT GCCATTGAGC 
CGCAGAGGGT GGAGTGGGTC AGAGTTGGAT CTAAAGGCAT CATTGACTGA AAGTGCCCAA 
AAGCGGGCTG ATCTGGTGCA GAGACTGAAA GATGCTCAGG TGAAGCTGGA GGGCCAATCA 
GAGGAGCTGC GGCACCGGGA TAAGGAGCTG GAGTTCAGCA GAGCTAAAAT TGAATTACTG 
GCAGTGAAGC AAAAGCAACT GGAAACATCA ATATCTCAGT TAGAGAAGGA AAAGGGTTGG 
TTAGAGGTGT CCCGCTATGA GGATAAGAGG CAACGTGGTG AACTGCAAGA CCGGATTATT 
AATCTAGAAA TGGAAGTGAT GAAGACCAAA TCTAACCTGG AAAATCTTAA CTACACCAGT 
GTACTTAAGT ATCAGAAGAC TCCAATGGAC TTAAAGGAAA ATGATGAACT AAGTCGAGAA 
CTAAGAACTG CACGTGAGAA TCTACTCTAC CTTCGAAATC GAGTCAAAGT CCTGGACGCA 
GAGAAGACTC AATCTGTTGA GGAACTAAGG ATATTGAGAG AGGGGAGTCA GCAGGCCTTT 
AGCCAAACCA ATGATGCAAA CCAGAGGGTA ACAGACACAC TCCGGGCTCA AAAAGATCTG 
CAAGAAGAGC TGGCAAATCT GCAACTGAAA CTAAACACTA CCAGTCTGGA GAAGGAGCTC 
GTGTCCTCAA AGATTATGAG ATTGGAGGAA AAGGTTAAAG ATCTGAATTT AAGGCTAAAT 
ATTGCACATT CTGACAGGGA ACGGGTTCTG CAGGACAAGG TGGAGCTGAC TAGGCGAAAT 
CAGGAATTGT CTCTCGAACT AGAACGGGCT CAGAGGGGTC AGGAGGGGTT TAATGACCAG 
GTCAGTGACC TTCACATTGA GCTGGTAGGA GCTAAAGCCC AGTCAAACCG TCAGGACCAG 
GAGAAGGTTC AGATGAAGGA GGAGCTGGTG ATGCTGAAAC AGGTGAATGA GAAACTGACT 
GCTGAACTGG GACAGACACA GCATACACTC CAGCGCACCC TGGATGAACT GCATCAGCTC 
CAGGCAGAAC ACAAGATTTC AACCAACCTT ACAGCTGCCT TGGAGGCAGA ACGTTCCCAG 
CTCTTGGGTG AGAAAGAGCT GCTGATGTCA ACTGTGGAGG AACATGGGGA GAGCCAGAGC 
ATCCAAGAGC TCAGATCTTC TCAGGCGCAG TTAATAGAAG AGAGGGATAA CCTACGTACC 
CATTGCCAGA ACCTGCAGGT TTCACTGGAA CAAGCACATG AACAGCTCGG CTCCCAGATC 
CAGGAGCAGC AGCAGCTCAC TCTGTATTGG AAGGAAAGGT GGCAGCAGAC TGCTGTTAAT 
TTAAAAAGCA CAGAAGAACA ACTGGAACAG GCAAAGGCTC TTTGCTCTAG TACAACAAAT 
AAGCACTCTG AGATCCTCCA AGATTATGAA CTCCTACAAA CAGATGCAGA GGAGCTGGCT 
GAACTCAGGA TGGCTATCAG CAGCCTGAAG AAAGAAAACA GCCTTTTAAA AGAGCAGATA 
AAGGAGCAGG AACAGAACAA GCATCTGCAG TGTCTTCAGA GCCACATCCA GGCTAATGTG 
GACAGCCCAG AGGATATGAA TTCGAAACTT GGCAAGCTGT ATAAAGAATT ACAGCAGGCT 
CACGAAAGGT TAAAATTCTT GGAGAGAGAA AGGTCTGAAA AAGAACTGGA AATAAAAAAA 
CTTAAGGCTG AGAGTGGATC CATACTTCGC ATTGAGCTAG ATGCCTGCAA ACAACAGTTG 
GAATTGGAGA GAAGTCGAAC AGCGACTCTA CAGGCCAAGA TTTCAGTCAT GGATTCCAGA 
AACAAAATGG AGGTTGCAAG TAGTCAGGAT CGTGTAGACA GTTTGCAGAA CCACAGCTTG 
TCCCTTCTAT CAGAAAGGTT AACATCAGAC GAATTGAGTT CTGAGATTGA GAATCTTCGT 
CAGCAGCTAG AAGAGGAGAG AGATGCAAAA AAGAAGAAAG AAGAACTGAT ATTTAATCTA 
AAAGAAGAGC TTGATGATCT CAAGCACAAG AAGCCTGGAG AAATCAAGGC ATCTCTGGAG 
GAAGTGGACT CAGAGCTAAT GTTTGTGCGA GAAGAACTGC AGAAAGTCTG GGAAATGTTG 
AAGACAAAAG ATACAGAACT TGAGGAACAG TATCAGGAGC TGGAGTCTGC TCGAGGCCAG 
TACACAGAGT GCAGCACAGA AAAAATGCGA CTGGAGCAAG TTCTTGCCAC CTTGCAGCAG 
CAGTTGACTG AGAAAGAGCA GACAATGAAA CATCTCAAGC AAATGAGAGA AATGGAGAAA 
ACTGAAATGG ATATTGAGAG GTCCTCATTG CAACTGAAGC TGGCAGAGCT ACAAGGACAA 
AAAGGAGATG TCACAACTCA GGCTGGGCAG CCGATAGAGG AGACTGGGCC TAAATGGTGG 
CGTTCAAAGG GAATGAAGGT TGTAGAGTCA TCAAAGAAAT GTATTCGCTG TGAAGCATTT 
TTGCAACAGT TGGATGAGAC AATCAAGGGT TGCCAGGAAC ACAATGCCGA GTTACAAGAA 
GAGAAGAACC AAACCTTGGC ATCCCTATAC CAGTTACAAG AAGTCCTAAC AAACCTCTCA 
AAACAATCAA AAGTAAATGA ACACGTGGCC CAGAGTCTAC AGGCGGACAA TGACTCTCTG 
AAAAAACAAC ACAGACTGGT GACAGAACAG CTTAAATGTC TGTTCAAAGA GAAAGAATCT 
CTGGGAAAAG CCTACCATAA GCTTCCAAAG AAGGATAAGC CAACGGAGGA CTGGAATGTT 
CAGTCCAGAT TAGTAAAGAA TGTGCTGAGT TCTATAAAAA GCCATAAAAA CCAGCAAGAA 
GAGCTAACAG AAGAGGCAGG CCATCAACCA ATGGAATTCG ATGCTGTGCA GATGGAAACT 
AATGAAAGAG ACATGAGGAA CTTGCAAAGA CAGCTTGAGG AAAAGTCTGA GCAGATATTG 
AAAATGGCTT CAGAAATGAA ACTGTTACAA GAAAAAAATG AGTCTTTAAT GAAAGCTAAG 
ATGCGTTTTC AACAACAAGT CCAGCAAATT CGGAGCATGC CACATCTAAG TCATCAAAGA 
GAAAAGACAG ATCCTACTGT ACCACCTCTG TCTGGAGACA TCAACACCTC CTTTCACAAA 
AAGAATGAGT TTTTGGCAGC AGAGAGCCAA GATTGTTCTG GTAGAATGCA GGGAACACCA 
ACTTCATTTC AGAGTGCCTC AGAGGGGTCC TGGACATCAT CACCTCAAAA CTCAAGACCT 
CCAACACCCA TCAACCAAAA GGTTATGCCA TCTGCATCTT TACTGAGAGT TCAGCTTCCT 
GGCACAGTGG GGAAACACTG GGTAAAATCA AAAGTGGAAT CTCCTTTAAG CCCCAGAAGC 
CTAGCAGACA GTGAAGGTAA CAGTGATACT CCAGGGTCAG TTACTCCAAG GACTTCTGCA 
TTGCTGTCTC CCAGACCTTT CCATCCACCA AAATCGGCAC AGGCCTTCAG ATATGGAAAG 
AATGTGCAAC ACACAAACAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100496327 ( XM_012966921.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822375.1 Xenopus tropicalis myosin-11-like (LOC100496327), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa36524
Clone ID Related Accession (Same CDS sequence)	XM_012966921.1
Accession Version	XM_012966921.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	myosin-11-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTATA GAGTGTGTAA CGGTTATGTG AGTTTGCCCT CCCGGTATGG AACAACTGGG 
AACTCTCAGT ATCATGTTGA CAGCGGCAGG ATATCTCCTT GCTGGTCCTA CAAAGATCTT 
GGCAGCAGCA GACATGGTTT GGAGTTATCC AAATCCCCCA TTTTATCTGA CCTAGAGAAA 
AATATTGAGA CAGCGGTGAA TTTAGAATCT AGCTTTCTGC CTGGGCAATG GAAAGATTAC 
AGCAGGAGTT CAGTAAGTCC ACATCATTCA GAGAGAGCGA GGTCTACAAG TCCAATGCGG 
GGGATCCACT CTCTAGAATG TTCCAGAATC CAGTCCACTC CTCCCATTTC AAGAAGAAAT 
TCACTTTCCC GTCTACCAAC TTCATCTTCT GAGGATTTCA GTCTTGAGCT GCCATTGAGC 
CGCAGAGGGT GGAGTGGGTC AGAGTTGGAT CTAAAGGCAT CATTGACTGA AAGTGCCCAA 
AAGCGGGCTG ATCTGGTGCA GAGACTGAAA GATGCTCAGG TGAAGCTGGA GGGCCAATCA 
GAGGAGCTGC GGCACCGGGA TAAGGAGCTG GAGTTCAGCA GAGCTAAAAT TGAATTACTG 
GCAGTGAAGC AAAAGCAACT GGAAACATCA ATATCTCAGT TAGAGAAGGA AAAGGGTTGG 
TTAGAGGTGT CCCGCTATGA GGATAAGAGG CAACGTGGTG AACTGCAAGA CCGGATTATT 
AATCTAGAAA TGGAAGTGAT GAAGACCAAA TCTAACCTGG AAAATCTTAA CTACACCAGT 
GTACTTAAGT ATCAGAAGAC TCCAATGGAA AATGATGAAC TAAGTCGAGA ACTAAGAACT 
GCACGTGAGA ATCTACTCTA CCTTCGAAAT CGAGTCAAAG TCCTGGACGC AGAGAAGACT 
CAATCTGTTG AGGAACTAAG GATATTGAGA GAGGGGAGTC AGCAGGCCTT TAGCCAAACC 
AATGATGCAA ACCAGAGGGT AACAGACACA CTCCGGGCTC AAAAAGATCT GCAAGAAGAG 
CTGGCAAATC TGCAACTGAA ACTAAACACT ACCAGTCTGG AGAAGGAGCT CGTGTCCTCA 
AAGATTATGA GATTGGAGGA AAAGGTTAAA GATCTGAATT TAAGGCTAAA TATTGCACAT 
TCTGACAGGG AACGGGTTCT GCAGGACAAG GTGGAGCTGA CTAGGCGAAA TCAGGAATTG 
TCTCTCGAAC TAGAACGGGC TCAGAGGGGT CAGGAGGGGT TTAATGACCA GGTCAGTGAC 
CTTCACATTG AGCTGGTAGG AGCTAAAGCC CAGTCAAACC GTCAGGACCA GGAGAAGGTT 
CAGATGAAGG AGGAGCTGGT GATGCTGAAA CAGGTGAATG AGAAACTGAC TGCTGAACTG 
GGACAGACAC AGCATACACT CCAGCGCACC CTGGATGAAC TGCATCAGCT CCAGGCAGAA 
CACAAGATTT CAACCAACCT TACAGCTGCC TTGGAGGCAG AACGTTCCCA GCTCTTGGGT 
GAGAAAGAGC TGCTGATGTC AACTGTGGAG GAACATGGGG AGAGCCAGAG CATCCAAGAG 
CTCAGATCTT CTCAGGCGCA GTTAATAGAA GAGAGGGATA ACCTACGTAC CCATTGCCAG 
AACCTGCAGG TTTCACTGGA ACAAGCACAT GAACAGCTCG GCTCCCAGAT CCAGGAGCAG 
CAGCAGCTCA CTCTGTATTG GAAGGAAAGG TGGCAGCAGA CTGCTGTTAA TTTAAAAAGC 
ACAGAAGAAC AACTGGAACA GGCAAAGGCT CTTTGCTCTA GTACAACAAA TAAGCACTCT 
GAGATCCTCC AAGATTATGA ACTCCTACAA ACAGATGCAG AGGAGCTGGC TGAACTCAGG 
ATGGCTATCA GCAGCCTGAA GAAAGAAAAC AGCCTTTTAA AAGAGCAGAT AAAGGAGCAG 
GAACAGAACA AGCATCTGCA GTGTCTTCAG AGCCACATCC AGGCTAATGT GGACAGCCCA 
GAGGATATGA ATTCGAAACT TGGCAAGCTG TATAAAGAAT TACAGCAGGC TCACGAAAGG 
TTAAAATTCT TGGAGAGAGA AAGGTCTGAA AAAGAACTGG AAATAAAAAA ACTTAAGGCT 
GAGAGTGGAT CCATACTTCG CATTGAGCTA GATGCCTGCA AACAACAGTT GGAATTGGAG 
AGAAGTCGAA CAGCGACTCT ACAGGCCAAG ATTTCAGTCA TGGATTCCAG AAACAAAATG 
GAGGTTGCAA GTAGTCAGGA TCGTGTAGAC AGTTTGCAGA ACCACAGCTT GTCCCTTCTA 
TCAGAAAGGT TAACATCAGA CGAATTGAGT TCTGAGATTG AGAATCTTCG TCAGCAGCTA 
GAAGAGGAGA GAGATGCAAA AAAGAAGAAA GAAGAACTGA TATTTAATCT AAAAGAAGAG 
CTTGATGATC TCAAGCACAA GAAGCCTGGA GAAATCAAGG CATCTCTGGA GGAAGTGGAC 
TCAGAGCTAA TGTTTGTGCG AGAAGAACTG CAGAAAGTCT GGGAAATGTT GAAGACAAAA 
GATACAGAAC TTGAGGAACA GTATCAGGAG CTGGAGTCTG CTCGAGGCCA GTACACAGAG 
TGCAGCACAG AAAAAATGCG ACTGGAGCAA GTTCTTGCCA CCTTGCAGCA GCAGTTGACT 
GAGAAAGAGC AGACAATGAA ACATCTCAAG CAAATGAGAG AAATGGAGAA AACTGAAATG 
GATATTGAGA GGTCCTCATT GCAACTGAAG CTGGCAGAGC TACAAGGACA AAAAGGAGAT 
GTCACAACTC AGGCTGGGCA GCCGATAGAG GAGACTGGGC CTAAATGGTG GCGTTCAAAG 
GGAATGAAGG TTGTAGAGTC ATCAAAGAAA TGTATTCGCT GTGAAGCATT TTTGCAACAG 
TTGGATGAGA CAATCAAGGG TTGCCAGGAA CACAATGCCG AGTTACAAGA AGAGAAGAAC 
CAAACCTTGG CATCCCTATA CCAGTTACAA GAAGTCCTAA CAAACCTCTC AAAACAATCA 
AAAGTAAATG AACACGTGGC CCAGAGTCTA CAGGCGGACA ATGACTCTCT GAAAAAACAA 
CACAGACTGG TGACAGAACA GCTTAAATGT CTGTTCAAAG AGAAAGAATC TCTGGGAAAA 
GCCTACCATA AGCTTCCAAA GAAGGATAAG CCAACGGAGG ACTGGAATGT TCAGTCCAGA 
TTAGTAAAGA ATGTGCTGAG TTCTATAAAA AGCCATAAAA ACCAGCAAGA AGAGCTAACA 
GAAGAGGCAG GCCATCAACC AATGGAATTC GATGCTGTGC AGATGGAAAC TAATGAAAGA 
GACATGAGGA ACTTGCAAAG ACAGCTTGAG GAAAAGTCTG AGCAGATATT GAAAATGGCT 
TCAGAAATGA AACTGTTACA AGAAAAAAAT GAGTCTTTAA TGAAAGCTAA GATGCGTTTT 
CAACAACAAG TCCAGCAAAT TCGGAGCATG CCACATCTAA GTCATCAAAG AGAAAAGACA 
GATCCTACTG TACCACCTCT GTCTGGAGAC ATCAACACCT CCTTTCACAA AAAGAATGAG 
TTTTTGGCAG CAGAGAGCCA AGATTGTTCT GGTAGAATGC AGGGAACACC AACTTCATTT 
CAGAGTGCCT CAGAGGGGTC CTGGACATCA TCACCTCAAA ACTCAAGACC TCCAACACCC 
ATCAACCAAA AGGTTATGCC ATCTGCATCT TTACTGAGAG TTCAGCTTCC TGGCACAGTG 
GGGAAACACT GGGTAAAATC AAAAGTGGAA TCTCCTTTAA GCCCCAGAAG CCTAGCAGAC 
AGTGAAGGTA ACAGTGATAC TCCAGGGTCA GTTACTCCAA GGACTTCTGC ATTGCTGTCT 
CCCAGACCTT TCCATCCACC AAAATCGGCA CAGGCCTTCA GATATGGAAA GAATGTGCAA 
CACACAAACA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822375.1
CDS	280..4194
Translation	MSYRVCNGYVSLPSRYGTTGNSQYHVDSGRISPCWSYKDLGSSRHGLELSKSPILSDLEKNIETAVNLESSFLPGQWKDYSRSSVSPHHSERARSTSPMRGIHSLECSRIQSTPPISRRNSLSRLPTSSSEDFSLELPLSRRGWSGSELDLKASLTESAQKRADLVQRLKDAQVKLEGQSEELRHRDKELEFSRAKIELLAVKQKQLETSISQLEKEKGWLEVSRYEDKRQRGELQDRIINLEMEVMKTKSNLENLNYTSVLKYQKTPMENDELSRELRTARENLLYLRNRVKVLDAEKTQSVEELRILREGSQQAFSQTNDANQRVTDTLRAQKDLQEELANLQLKLNTTSLEKELVSSKIMRLEEKVKDLNLRLNIAHSDRERVLQDKVELTRRNQELSLELERAQRGQEGFNDQVSDLHIELVGAKAQSNRQDQEKVQMKEELVMLKQVNEKLTAELGQTQHTLQRTLDELHQLQAEHKISTNLTAALEAERSQLLGEKELLMSTVEEHGESQSIQELRSSQAQLIEERDNLRTHCQNLQVSLEQAHEQLGSQIQEQQQLTLYWKERWQQTAVNLKSTEEQLEQAKALCSSTTNKHSEILQDYELLQTDAEELAELRMAISSLKKENSLLKEQIKEQEQNKHLQCLQSHIQANVDSPEDMNSKLGKLYKELQQAHERLKFLERERSEKELEIKKLKAESGSILRIELDACKQQLELERSRTATLQAKISVMDSRNKMEVASSQDRVDSLQNHSLSLLSERLTSDELSSEIENLRQQLEEERDAKKKKEELIFNLKEELDDLKHKKPGEIKASLEEVDSELMFVREELQKVWEMLKTKDTELEEQYQELESARGQYTECSTEKMRLEQVLATLQQQLTEKEQTMKHLKQMREMEKTEMDIERSSLQLKLAELQGQKGDVTTQAGQPIEETGPKWWRSKGMKVVESSKKCIRCEAFLQQLDETIKGCQEHNAELQEEKNQTLASLYQLQEVLTNLSKQSKVNEHVAQSLQADNDSLKKQHRLVTEQLKCLFKEKESLGKAYHKLPKKDKPTEDWNVQSRLVKNVLSSIKSHKNQQEELTEEAGHQPMEFDAVQMETNERDMRNLQRQLEEKSEQILKMASEMKLLQEKNESLMKAKMRFQQQVQQIRSMPHLSHQREKTDPTVPPLSGDINTSFHKKNEFLAAESQDCSGRMQGTPTSFQSASEGSWTSSPQNSRPPTPINQKVMPSASLLRVQLPGTVGKHWVKSKVESPLSPRSLADSEGNSDTPGSVTPRTSALLSPRPFHPPKSAQAFRYGKNVQHTNS

Target ORF information:

RefSeq Version	XM_012966921.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin-11-like (LOC100496327), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966921.1

ORF Insert Sequence:

ATGTCTTATA GAGTGTGTAA CGGTTATGTG AGTTTGCCCT CCCGGTATGG AACAACTGGG 
AACTCTCAGT ATCATGTTGA CAGCGGCAGG ATATCTCCTT GCTGGTCCTA CAAAGATCTT 
GGCAGCAGCA GACATGGTTT GGAGTTATCC AAATCCCCCA TTTTATCTGA CCTAGAGAAA 
AATATTGAGA CAGCGGTGAA TTTAGAATCT AGCTTTCTGC CTGGGCAATG GAAAGATTAC 
AGCAGGAGTT CAGTAAGTCC ACATCATTCA GAGAGAGCGA GGTCTACAAG TCCAATGCGG 
GGGATCCACT CTCTAGAATG TTCCAGAATC CAGTCCACTC CTCCCATTTC AAGAAGAAAT 
TCACTTTCCC GTCTACCAAC TTCATCTTCT GAGGATTTCA GTCTTGAGCT GCCATTGAGC 
CGCAGAGGGT GGAGTGGGTC AGAGTTGGAT CTAAAGGCAT CATTGACTGA AAGTGCCCAA 
AAGCGGGCTG ATCTGGTGCA GAGACTGAAA GATGCTCAGG TGAAGCTGGA GGGCCAATCA 
GAGGAGCTGC GGCACCGGGA TAAGGAGCTG GAGTTCAGCA GAGCTAAAAT TGAATTACTG 
GCAGTGAAGC AAAAGCAACT GGAAACATCA ATATCTCAGT TAGAGAAGGA AAAGGGTTGG 
TTAGAGGTGT CCCGCTATGA GGATAAGAGG CAACGTGGTG AACTGCAAGA CCGGATTATT 
AATCTAGAAA TGGAAGTGAT GAAGACCAAA TCTAACCTGG AAAATCTTAA CTACACCAGT 
GTACTTAAGT ATCAGAAGAC TCCAATGGAA AATGATGAAC TAAGTCGAGA ACTAAGAACT 
GCACGTGAGA ATCTACTCTA CCTTCGAAAT CGAGTCAAAG TCCTGGACGC AGAGAAGACT 
CAATCTGTTG AGGAACTAAG GATATTGAGA GAGGGGAGTC AGCAGGCCTT TAGCCAAACC 
AATGATGCAA ACCAGAGGGT AACAGACACA CTCCGGGCTC AAAAAGATCT GCAAGAAGAG 
CTGGCAAATC TGCAACTGAA ACTAAACACT ACCAGTCTGG AGAAGGAGCT CGTGTCCTCA 
AAGATTATGA GATTGGAGGA AAAGGTTAAA GATCTGAATT TAAGGCTAAA TATTGCACAT 
TCTGACAGGG AACGGGTTCT GCAGGACAAG GTGGAGCTGA CTAGGCGAAA TCAGGAATTG 
TCTCTCGAAC TAGAACGGGC TCAGAGGGGT CAGGAGGGGT TTAATGACCA GGTCAGTGAC 
CTTCACATTG AGCTGGTAGG AGCTAAAGCC CAGTCAAACC GTCAGGACCA GGAGAAGGTT 
CAGATGAAGG AGGAGCTGGT GATGCTGAAA CAGGTGAATG AGAAACTGAC TGCTGAACTG 
GGACAGACAC AGCATACACT CCAGCGCACC CTGGATGAAC TGCATCAGCT CCAGGCAGAA 
CACAAGATTT CAACCAACCT TACAGCTGCC TTGGAGGCAG AACGTTCCCA GCTCTTGGGT 
GAGAAAGAGC TGCTGATGTC AACTGTGGAG GAACATGGGG AGAGCCAGAG CATCCAAGAG 
CTCAGATCTT CTCAGGCGCA GTTAATAGAA GAGAGGGATA ACCTACGTAC CCATTGCCAG 
AACCTGCAGG TTTCACTGGA ACAAGCACAT GAACAGCTCG GCTCCCAGAT CCAGGAGCAG 
CAGCAGCTCA CTCTGTATTG GAAGGAAAGG TGGCAGCAGA CTGCTGTTAA TTTAAAAAGC 
ACAGAAGAAC AACTGGAACA GGCAAAGGCT CTTTGCTCTA GTACAACAAA TAAGCACTCT 
GAGATCCTCC AAGATTATGA ACTCCTACAA ACAGATGCAG AGGAGCTGGC TGAACTCAGG 
ATGGCTATCA GCAGCCTGAA GAAAGAAAAC AGCCTTTTAA AAGAGCAGAT AAAGGAGCAG 
GAACAGAACA AGCATCTGCA GTGTCTTCAG AGCCACATCC AGGCTAATGT GGACAGCCCA 
GAGGATATGA ATTCGAAACT TGGCAAGCTG TATAAAGAAT TACAGCAGGC TCACGAAAGG 
TTAAAATTCT TGGAGAGAGA AAGGTCTGAA AAAGAACTGG AAATAAAAAA ACTTAAGGCT 
GAGAGTGGAT CCATACTTCG CATTGAGCTA GATGCCTGCA AACAACAGTT GGAATTGGAG 
AGAAGTCGAA CAGCGACTCT ACAGGCCAAG ATTTCAGTCA TGGATTCCAG AAACAAAATG 
GAGGTTGCAA GTAGTCAGGA TCGTGTAGAC AGTTTGCAGA ACCACAGCTT GTCCCTTCTA 
TCAGAAAGGT TAACATCAGA CGAATTGAGT TCTGAGATTG AGAATCTTCG TCAGCAGCTA 
GAAGAGGAGA GAGATGCAAA AAAGAAGAAA GAAGAACTGA TATTTAATCT AAAAGAAGAG 
CTTGATGATC TCAAGCACAA GAAGCCTGGA GAAATCAAGG CATCTCTGGA GGAAGTGGAC 
TCAGAGCTAA TGTTTGTGCG AGAAGAACTG CAGAAAGTCT GGGAAATGTT GAAGACAAAA 
GATACAGAAC TTGAGGAACA GTATCAGGAG CTGGAGTCTG CTCGAGGCCA GTACACAGAG 
TGCAGCACAG AAAAAATGCG ACTGGAGCAA GTTCTTGCCA CCTTGCAGCA GCAGTTGACT 
GAGAAAGAGC AGACAATGAA ACATCTCAAG CAAATGAGAG AAATGGAGAA AACTGAAATG 
GATATTGAGA GGTCCTCATT GCAACTGAAG CTGGCAGAGC TACAAGGACA AAAAGGAGAT 
GTCACAACTC AGGCTGGGCA GCCGATAGAG GAGACTGGGC CTAAATGGTG GCGTTCAAAG 
GGAATGAAGG TTGTAGAGTC ATCAAAGAAA TGTATTCGCT GTGAAGCATT TTTGCAACAG 
TTGGATGAGA CAATCAAGGG TTGCCAGGAA CACAATGCCG AGTTACAAGA AGAGAAGAAC 
CAAACCTTGG CATCCCTATA CCAGTTACAA GAAGTCCTAA CAAACCTCTC AAAACAATCA 
AAAGTAAATG AACACGTGGC CCAGAGTCTA CAGGCGGACA ATGACTCTCT GAAAAAACAA 
CACAGACTGG TGACAGAACA GCTTAAATGT CTGTTCAAAG AGAAAGAATC TCTGGGAAAA 
GCCTACCATA AGCTTCCAAA GAAGGATAAG CCAACGGAGG ACTGGAATGT TCAGTCCAGA 
TTAGTAAAGA ATGTGCTGAG TTCTATAAAA AGCCATAAAA ACCAGCAAGA AGAGCTAACA 
GAAGAGGCAG GCCATCAACC AATGGAATTC GATGCTGTGC AGATGGAAAC TAATGAAAGA 
GACATGAGGA ACTTGCAAAG ACAGCTTGAG GAAAAGTCTG AGCAGATATT GAAAATGGCT 
TCAGAAATGA AACTGTTACA AGAAAAAAAT GAGTCTTTAA TGAAAGCTAA GATGCGTTTT 
CAACAACAAG TCCAGCAAAT TCGGAGCATG CCACATCTAA GTCATCAAAG AGAAAAGACA 
GATCCTACTG TACCACCTCT GTCTGGAGAC ATCAACACCT CCTTTCACAA AAAGAATGAG 
TTTTTGGCAG CAGAGAGCCA AGATTGTTCT GGTAGAATGC AGGGAACACC AACTTCATTT 
CAGAGTGCCT CAGAGGGGTC CTGGACATCA TCACCTCAAA ACTCAAGACC TCCAACACCC 
ATCAACCAAA AGGTTATGCC ATCTGCATCT TTACTGAGAG TTCAGCTTCC TGGCACAGTG 
GGGAAACACT GGGTAAAATC AAAAGTGGAA TCTCCTTTAA GCCCCAGAAG CCTAGCAGAC 
AGTGAAGGTA ACAGTGATAC TCCAGGGTCA GTTACTCCAA GGACTTCTGC ATTGCTGTCT 
CCCAGACCTT TCCATCCACC AAAATCGGCA CAGGCCTTCA GATATGGAAA GAATGTGCAA 
CACACAAACA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC100496327 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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