dennd4c cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	dennd4c
Entrez Gene ID	100494441
Full Name	DENN domain containing 4C
General protein information	Preferred Names DENN domain containing 4C Names DENN domain-containing protein 4C DENN/MADD domain containing 4C
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004910862.4	XP_004910919.1	DENN domain-containing protein 4C
XM_004910863.3	XP_004910920.1	DENN domain-containing protein 4C isoform X1
XM_004910863.4	XP_004910920.1	DENN domain-containing protein 4C
XM_004910862.3	XP_004910919.1	DENN domain-containing protein 4C isoform X1
XM_002935667.4	XP_002935713.2	DENN domain-containing protein 4C isoform X1
XM_018097779.1	XP_017953268.1	DENN domain-containing protein 4C isoform X2
XM_002935667.5	XP_002935713.2	DENN domain-containing protein 4C

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DENND4C	XP_005626722.1
	LOC712447	XP_001101451.2
Gallus gallus (chicken)	DENND4C	XP_004949183.1
Mus musculus (house mouse)	Dennd4c	NP_908976.1
Rattus norvegicus (Norway rat)	RGD1308489	XP_006238437.1
Xenopus tropicalis (tropical clawed frog)	dennd4c	XP_004910919.1
Pan troglodytes (chimpanzee)	DENND4C	XP_003312065.1
Bos taurus (cattle)	DENND4C	XP_002689611.3
Danio rerio (zebrafish)	dennd4c	XP_005166610.1
Homo sapiens (human)	DENND4C	NP_060395.5

Gene Ontology

The following dennd4c gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dennd4c cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa04068	XM_002935667.5 Latest version!	Xenopus tropicalis DENN domain containing 4C (dennd4c), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa04068	XM_004910863.4 Latest version!	Xenopus tropicalis DENN domain containing 4C (dennd4c), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa04068	XM_004910862.4 Latest version!	Xenopus tropicalis DENN domain containing 4C (dennd4c), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa42090	XM_018097779.1 Latest version!	Xenopus tropicalis DENN/MADD domain containing 4C (dennd4c), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
View Old Accession Versions >>
OXa04068	XM_004910863.3	Xenopus tropicalis DENN/MADD domain containing 4C (dennd4c), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa04068	XM_004910862.3	Xenopus tropicalis DENN/MADD domain containing 4C (dennd4c), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa04068	XM_002935667.4	Xenopus tropicalis DENN/MADD domain containing 4C (dennd4c), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dennd4c ( XM_004910862.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910919.1 Xenopus tropicalis DENN domain containing 4C (dennd4c), transcript variant X1, mRNA.

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CloneID	OXa04068
Clone ID Related Accession (Same CDS sequence)	XM_004910862.4 , XM_004910863.3 , XM_004910863.4 , XM_004910862.3 , XM_002935667.4 , XM_002935667.5
Accession Version	XM_004910862.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DENN domain-containing protein 4C
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910862.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG ACAAAGGGCC TCGGGTAGCA GACTACTTTG TTGTGGCAGG ACTGACAGAC 
ACCTTCAAGC TATTAGATCA TGACATGGGT CGGATAGAAA CTAAGACATT AGGTCCCAAA 
GCTCCAATCA CTGATATTGC TGTTATCAAC AAGACAGCTG GAGAGACCGT GCCAGAGGGC 
TACACATGTA TTGAAATCAC ACAAAGTGGG TTGCAGGCAA ATCTGAACCA TGGAAGCTTA 
AAGAGTCCAG AAATGTTTAT CTGTTACAGA CGAGAAAAAG GGAAACCTCC ACTAACAGAC 
ATTGGTGTGT TATATGAAGG AAAAGAACAC CTATTGAAAG GCTGTGAGGT TATCCAGGCT 
ACACCATATG GACGCTGCGC AAATGTCAAT AACAGCTCGG GTACCTCCAT GAGGATATTT 
CTAACTTACC GACGATCTTT ACCTGTTCGC CTTCAGAACT CCTTGGCAGT AACTGACATA 
TGTATAATCA TCAGTAATAA AGGAGAGACA CCACCCCATA CCTTCTGTAA GGTGGACAAA 
AACCTCAACT GTGGAATGTG GGGATCCAAT GTTTTCCTGT GTTATAAGAA GTCTGTCCCT 
GCATCTAACT CAATTCCTTA TAAAGCTGGT TTGATTTTCA GATATCCAGA GGAGGATAAC 
GAGTCATTTC CTCTATCAGA GTCCGTGCCA CTCTTTTGCC TTCCCATGGG AGCCACTATT 
GAATGCTGGG ACCCTCAAAT CCGACATCCT CTCCCTGTTT TCTCTACTTT TGTATTGACA 
GGTTCCACAG CTGAAAAGGT TTATGGTGCT GCTATTCAGT TCTATGAGAC TTACCCACGT 
GAAATGTTGT CTGAGAAGCA GATGATTCAG TTAGGACTGA TCACAGCTGT GGAGAAGAAA 
GTGCTCACCT ACAAAAATAT TAACTCCAAG AAGTGCATAT GTTTGCTTTC TCACTGGCCT 
TTCTTTGAGA CCTTCCGCAA GTTTCTTATG TTCATCTACA AGCTGTCTGT GTCTGGCCCT 
CATACCCTTC CTATTGAAAA GCACATATCG CACTTTATGC ACAATGTTCC CTTCCCATCA 
CCACAGAGGC CAAGAATTCT TGTTCAGCTC TCTGCTCATG ATGCCTTAGT TCTGTCCCAG 
CCAGTAGCAA CACCAATGCC TCTAAGTGGA GCAAATTTTA GTACACTGCT TATGAATCTT 
GGACCAGAAA ACTGTGCTTC TTTGCTGTTG TTCGTACTGC TAGAATGCAA GATTCTCCTT 
CACTCCCTCA GGCCAGCAGT GCTGACTGGA GTTGCTGAGG CTGTAGTAGC TATGATTTTC 
CCTTTCCAGT GGCAGTGCCC TTACATCCCC CTGTGCCCAC TGTCTCTCGC GGATGTGCTT 
AGTGCACCTA TTCCCTTTAT TGTGGGAGTG GATTCTCGAT ACTTTGATTT GTATGACCCG 
CCTCAGGATG TTGTTTGCAT CGACTTGGAC ACAAGCATGG TTTACACGTC TGAAGACAAG 
AGGAATTTAA GCTGGAAACA GCTTCCCAAG AAACCAGTGA AAAACCTTAT TGGCGTCTTA 
AAAAAGCTTT ACCCACGACT CTCTTCAGTT CACCAAAAGT CTCCAGAAGA GTCTGCTGTG 
CTGATGGCCC CAATTGAGGC AGACTTCTCA TGGCAAAAGA AAACAACAGA TTTGGAGATG 
GAAATTCAAG AGGCATTTCT ACGGTTCATG GCATCCATAC TCAAAGGTTA TAGGGCTTAC 
TTGAAACCCA TAACTCAAGC ACCATCCAAC AAAGCTACTG CTGCAGATTC ACTGTTTGAC 
CGTCAAGGGT TTTTGAAAAG TAGGGATCGT GCACACATAA AATTCTACAC TTCACTGACA 
AAAACCCAGA TTTTCATCCG CTTCATTGAA GAGTGCAGCT TTGTCAGTGA TAAGGACACC 
AGCCTGGCCT TCTTTGATGA CTGCATTGAT AAGTTGTTTC CTGATAAAGG AGTGGAGAGA 
GGTGATAAGG GGGATCTGGA GTTTCCAGAA GACACTCGTC TGATTGAGCT AGATGACTCA 
CAGAAGAGCG AGCACACTGT GTTTATAATG CCACCTGAGC CTCTGCCGGA AGATGGACCT 
GACCCTCACC CAAGATATAG CTACAAAAAC TTTCCAAGAC TAGACTACGC TCTTTTTGAC 
CGAATACGTG ATCAATATCT TACTTTCAAT GGGAATACTA AGAGTAGCAG AGCAGCAAGC 
AGCCCCACAT TTATTGCAAA AAGGACAAAA CAGGAAATCA AAACGGCTCA CAAGTTAGCT 
AGAAAATATT TTAGCAACGA TCCTGCTTGG GGCAAATGTC TCTTCAGCCA GTGCTTCAGT 
TTGTGGTTTA TTTGCCTGCC TGTTTATGTT AAGGTATCAC ATTCAAAAGC ACGTGCACTT 
CAGAAGGCTT ATGATGTGCT GGTTAAGATG AGGAAAACTG ATGTAGATCC ATTGGATGAG 
GTGTGTTACA GAGTGGTAAT GCAACTGTGT GGCATTTGGG AGAACCCTGC TCTGGCAGTG 
AGAGTATTAT TTGAGATGAA AAACGCTGGG ATACAGCCAA ATGCAATTAC CTATGGTTAC 
TATAATAAGG TTGTGTTGGT CAGCCCATGG CCAAGCAGTA ATCGCAGTGG AAACTTCCTA 
TGGACAAAAG TGCGCAATGT GGTGTTTGGT TTGGCTCAGT TCCGAAGGGC AACGAAGAAG 
CCACAGACAT CTTCCGAGTC CTCTTCGGCA TCAGCGCTTC CAAGTGCTGC ACATGCAAAT 
GATGGCAATG CTGTGAGCCG ACAAAAAGTG GATAATTATA ACGATTCTAA CTCTGGGGAG 
CACACCCTTT TCGTGCGCGA TCTAATCAGA ACTGATTCCA CTGATAACCA TTCTAGCACA 
GAGAGTCCAT CTAAAAATGG CTCCAAAGAC AAGGTCGAGA CAGATGATGC TCAGAGTGGT 
TCTGATCAGA AAAACAAAGC AGAAACAGAC GCTAAAGCAA AGCAGAAACA CACACCAGAC 
TGTGATGAGC CTTCACTTTC CTCTATGGAT CCAACAGTAT CTAGTGAAGG ACATCCAGAA 
AAATTACATT CAACCTCTGA GGTTCCAAAT CCTCCTGAAT CACCTCCTTG TGCAAGTAGC 
ATTGTTAAAG TACCTTCTGG AATTTTTGAC AGCACAGGAA GGAAAAGCAG TACCGGAGGT 
GGAACTGGAC CATTATCCCC GGTTTCAGAT GTTGGGGAAG ACCATGTGTT TTCCAGCGAA 
GGTCAAAACA AGAAGTCCAG TGAGAGAGCT CAAAAGAGGC AGAAAATGTT TGCTGAGCGA 
AGCTGTAGTT TCAGCGCAGA GAGCCGAGCT GGTATGCTAC TAAAGAAAGG CAGTCTTGAC 
CTAACCACTA ATGAAATTGC CATAAAGATG GGAGCGGATG CAAAAATACT TACTGCAGCT 
CTTTCAAAGA CACCACCACC CCAGCATGCT AAGAATGAAT TCTGCTTTGA AAGTATAGGT 
GAACAGTCTG TCTGTCAGAC ATCTGATAGC ACAGCTTGTG AACAAGATGA AAAACCTGAC 
ACAGAGACTG AGGAGCTAAC AGCCTCACGA CTGCAGGGGC CATCTGATCA GTTAGATACG 
GTGAAAGAGG AAAGCACAGT GGATGCTGGG GACTCTGTTA TAGGCACAGT GAAAGACAGC 
GTTGCTCTCA GGGATACTCC TAAACTTATA AGTGAACTTA ATAAAAGGCA TAGTTTTTAT 
GGCCTCACTA AATCCGTAGA AAGAGAAGGG GTAGAAAAGG GTCTTGACCC TCTATCTTTA 
ATGGCTTCAC AGACAGTGGA GAAAGAGCCA TCACCATCTG AGGAAAAGCA CATTTCCCCT 
GTTGTGGCAC GTAATCTGGC CGATGAGATT GAAAGTTACA TGAACCTGAA AAGTCCTCTT 
GGCTCCAAGT CTTCAAGTAT GGAGCTAGGA GGACAGGAGT GCAATGCAAG GGAATCTGGG 
AACCACAGCC AAGCTGGCCT GGAGCGGCGC TCTAGCTTAC CCGTAGACTC TTTTCCTCCA 
TCAGAAGTGC AGAGTGAAAA CCAGAAGAAC TCAGCTGTAT CCAGATCCAA GACTTTTACT 
AGCAGTCCAA AGCATCACCT ACGCAACCAA AAGGAACGAT CAGTTTCACT CACTGCCCTT 
GTCCGCAGCT CCCCACATGG CTCTTTGGGT TCGATGGTTA ACTCCTTTCC TGGGCTAAAA 
TTTGACAACC TTCTGACAGG ACCTAAAATG GATGTGCTGA AATCAGGCAT GAAGCAGGCA 
GCCACTGTAG CAAGTAAAGT TTGGGGGGCT GTTGCATCCG CTTATAGCTA CTCTGATGAT 
GAGGAGGAAA GTCAAAGTCA GAGAGGTCAG TCTAACTTTC CTGTAAGACC TCAAGAGTCT 
GGTGTTGGTG ACAATCTTTC CTTCAAGATG CCTTCAAATG GACTTTCTGG CAATGAGCTG 
ACACAGAGCA ATACCAGCTT AGGCAGCAGC AGCAGTGGAG ACACAAGCAA GCACAGCTAC 
TTAAATGAAT CTGTACAGGG GAAAGCATCA AAGGTTCTAG AGTCTGAAAA ATCAGAGCAA 
GGATCCTCTC ACAGCACCAG CTTGTCAAGC ATATTTCAGA ACTGGGCAAT GGAGGTCTTG 
ATGTCTAGTT GCTCTCAGTG CCGAGGTTGT GATGCACTTG TGTATGATGA AGAGATAATG 
GCTGGATGGA CTTTTGATGA CTCAAACTTA AATACTACTT GTCCCTTTTG TAAAAGACCC 
TTTTTACCAC TTCTTAATAT TGAGTTTAAA GACCTTCGTG GTACTGCAAG CTTTTTTCTT 
AAGCCAAGTA ACTCAGGAGA CAGTCTTCAT GGCAGTAACC TTCGGGTAAT GGCAGATTCG 
TCAGAGCGTA AACTGCAATC AGAATCTACA GCCAGTGACC TGATCAGCTT TGGGGAATCT 
TCCACTACTC CCCAAACCAC AGCAGAGGAA AGTAATACAG ACACTCAGCA GAGTGACCAG 
GCTGAAGAAG ATGGAACCAT GAAGTCTTCT GAGACAAACA ATGCACCAAA GCGAGGAGCG 
TCACTGACTA GGAGTCACAG CGTAGGAGGG CCTCTACAAA ACATTGACTT ATCCCAAAGG 
CCTTCTCATG GAATTTCCAC TGTCAGTCTT CCCAACAGCC TGCAAGAAGC TGTGGATCCT 
TTGGAAAAGC GGCACAACCC TCCTCCTGTC TCCGTCCCCT ACCTTAGTCC CCTTGTCCTA 
CGCAAGGAGC TGGAATCTTT GCTGGAGAAT GAGGGCGACC ATGTCATCTA CACATCCACT 
TTTATTAACC AGCATCCTAT TATCTTCTGG AACCTGGTTT GGTTCTTTAG ACGTTTGGAC 
CTGCCCAGCA ATTTGCCAGG GCTCATCCTC TCATCTGAGC ACTGCAATGG AGGGGTGCAG 
CTCCCGCTAT CATCTCTATC TCAGGACAGC AAGCTAGTCT ATATACAACT GTTGTGGGAC 
AACATAAACC TTCACCAGGA GCCCGGGGAG CCCCTTTACA GCAAATTTAG AGCCCTGGAC 
AATATCAAGA AGAAGGCTTC TCCTATCCCA TATGATCAAC AAAGCACCAA CAATTTACTG 
GAAAGCATTA CGCTTAGTAT CCAGCATAAT GACGTCCTTA GGCCTATCAG CCTGCTCCTG 
CAGAAATGCA GCACAACTGG AAAGAGGCAA AGGAGCTTGT ATAGAGAAAT ACTGTACCTT 
TCTTTGGTGT CTCTTGGAAG AGAAAATATC GACATAGAGG CCTTTGACAA TGAATATAGA 
ATCGCTTATG AAAAGCTCCC TCTTGAGATG CAGCAAAAAA TGCAGAAAAT TGATCAGCCT 
CCAAGCAAGA AGGTGGAATG CTGCAGGAGT ATCTTTGGAG CGCCCCTAAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910919.1
CDS	225..6098
Translation	MMEDKGPRVADYFVVAGLTDTFKLLDHDMGRIETKTLGPKAPITDIAVINKTAGETVPEGYTCIEITQSGLQANLNHGSLKSPEMFICYRREKGKPPLTDIGVLYEGKEHLLKGCEVIQATPYGRCANVNNSSGTSMRIFLTYRRSLPVRLQNSLAVTDICIIISNKGETPPHTFCKVDKNLNCGMWGSNVFLCYKKSVPASNSIPYKAGLIFRYPEEDNESFPLSESVPLFCLPMGATIECWDPQIRHPLPVFSTFVLTGSTAEKVYGAAIQFYETYPREMLSEKQMIQLGLITAVEKKVLTYKNINSKKCICLLSHWPFFETFRKFLMFIYKLSVSGPHTLPIEKHISHFMHNVPFPSPQRPRILVQLSAHDALVLSQPVATPMPLSGANFSTLLMNLGPENCASLLLFVLLECKILLHSLRPAVLTGVAEAVVAMIFPFQWQCPYIPLCPLSLADVLSAPIPFIVGVDSRYFDLYDPPQDVVCIDLDTSMVYTSEDKRNLSWKQLPKKPVKNLIGVLKKLYPRLSSVHQKSPEESAVLMAPIEADFSWQKKTTDLEMEIQEAFLRFMASILKGYRAYLKPITQAPSNKATAADSLFDRQGFLKSRDRAHIKFYTSLTKTQIFIRFIEECSFVSDKDTSLAFFDDCIDKLFPDKGVERGDKGDLEFPEDTRLIELDDSQKSEHTVFIMPPEPLPEDGPDPHPRYSYKNFPRLDYALFDRIRDQYLTFNGNTKSSRAASSPTFIAKRTKQEIKTAHKLARKYFSNDPAWGKCLFSQCFSLWFICLPVYVKVSHSKARALQKAYDVLVKMRKTDVDPLDEVCYRVVMQLCGIWENPALAVRVLFEMKNAGIQPNAITYGYYNKVVLVSPWPSSNRSGNFLWTKVRNVVFGLAQFRRATKKPQTSSESSSASALPSAAHANDGNAVSRQKVDNYNDSNSGEHTLFVRDLIRTDSTDNHSSTESPSKNGSKDKVETDDAQSGSDQKNKAETDAKAKQKHTPDCDEPSLSSMDPTVSSEGHPEKLHSTSEVPNPPESPPCASSIVKVPSGIFDSTGRKSSTGGGTGPLSPVSDVGEDHVFSSEGQNKKSSERAQKRQKMFAERSCSFSAESRAGMLLKKGSLDLTTNEIAIKMGADAKILTAALSKTPPPQHAKNEFCFESIGEQSVCQTSDSTACEQDEKPDTETEELTASRLQGPSDQLDTVKEESTVDAGDSVIGTVKDSVALRDTPKLISELNKRHSFYGLTKSVEREGVEKGLDPLSLMASQTVEKEPSPSEEKHISPVVARNLADEIESYMNLKSPLGSKSSSMELGGQECNARESGNHSQAGLERRSSLPVDSFPPSEVQSENQKNSAVSRSKTFTSSPKHHLRNQKERSVSLTALVRSSPHGSLGSMVNSFPGLKFDNLLTGPKMDVLKSGMKQAATVASKVWGAVASAYSYSDDEEESQSQRGQSNFPVRPQESGVGDNLSFKMPSNGLSGNELTQSNTSLGSSSSGDTSKHSYLNESVQGKASKVLESEKSEQGSSHSTSLSSIFQNWAMEVLMSSCSQCRGCDALVYDEEIMAGWTFDDSNLNTTCPFCKRPFLPLLNIEFKDLRGTASFFLKPSNSGDSLHGSNLRVMADSSERKLQSESTASDLISFGESSTTPQTTAEESNTDTQQSDQAEEDGTMKSSETNNAPKRGASLTRSHSVGGPLQNIDLSQRPSHGISTVSLPNSLQEAVDPLEKRHNPPPVSVPYLSPLVLRKELESLLENEGDHVIYTSTFINQHPIIFWNLVWFFRRLDLPSNLPGLILSSEHCNGGVQLPLSSLSQDSKLVYIQLLWDNINLHQEPGEPLYSKFRALDNIKKKASPIPYDQQSTNNLLESITLSIQHNDVLRPISLLLQKCSTTGKRQRSLYREILYLSLVSLGRENIDIEAFDNEYRIAYEKLPLEMQQKMQKIDQPPSKKVECCRSIFGAPLI

Target ORF information:

RefSeq Version	XM_004910862.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN domain containing 4C (dennd4c), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910862.4

ORF Insert Sequence:

ATGATGGAAG ACAAAGGGCC TCGGGTAGCA GACTACTTTG TTGTGGCAGG ACTGACAGAC 
ACCTTCAAGC TATTAGATCA TGACATGGGT CGGATAGAAA CTAAGACATT AGGTCCCAAA 
GCTCCAATCA CTGATATTGC TGTTATCAAC AAGACAGCTG GAGAGACCGT GCCAGAGGGC 
TACACATGTA TTGAAATCAC ACAAAGTGGG TTGCAGGCAA ATCTGAACCA TGGAAGCTTA 
AAGAGTCCAG AAATGTTTAT CTGTTACAGA CGAGAAAAAG GGAAACCTCC ACTAACAGAC 
ATTGGTGTGT TATATGAAGG AAAAGAACAC CTATTGAAAG GCTGTGAGGT TATCCAGGCT 
ACACCATATG GACGCTGCGC AAATGTCAAT AACAGCTCGG GTACCTCCAT GAGGATATTT 
CTAACTTACC GACGATCTTT ACCTGTTCGC CTTCAGAACT CCTTGGCAGT AACTGACATA 
TGTATAATCA TCAGTAATAA AGGAGAGACA CCACCCCATA CCTTCTGTAA GGTGGACAAA 
AACCTCAACT GTGGAATGTG GGGATCCAAT GTTTTCCTGT GTTATAAGAA GTCTGTCCCT 
GCATCTAACT CAATTCCTTA TAAAGCTGGT TTGATTTTCA GATATCCAGA GGAGGATAAC 
GAGTCATTTC CTCTATCAGA GTCCGTGCCA CTCTTTTGCC TTCCCATGGG AGCCACTATT 
GAATGCTGGG ACCCTCAAAT CCGACATCCT CTCCCTGTTT TCTCTACTTT TGTATTGACA 
GGTTCCACAG CTGAAAAGGT TTATGGTGCT GCTATTCAGT TCTATGAGAC TTACCCACGT 
GAAATGTTGT CTGAGAAGCA GATGATTCAG TTAGGACTGA TCACAGCTGT GGAGAAGAAA 
GTGCTCACCT ACAAAAATAT TAACTCCAAG AAGTGCATAT GTTTGCTTTC TCACTGGCCT 
TTCTTTGAGA CCTTCCGCAA GTTTCTTATG TTCATCTACA AGCTGTCTGT GTCTGGCCCT 
CATACCCTTC CTATTGAAAA GCACATATCG CACTTTATGC ACAATGTTCC CTTCCCATCA 
CCACAGAGGC CAAGAATTCT TGTTCAGCTC TCTGCTCATG ATGCCTTAGT TCTGTCCCAG 
CCAGTAGCAA CACCAATGCC TCTAAGTGGA GCAAATTTTA GTACACTGCT TATGAATCTT 
GGACCAGAAA ACTGTGCTTC TTTGCTGTTG TTCGTACTGC TAGAATGCAA GATTCTCCTT 
CACTCCCTCA GGCCAGCAGT GCTGACTGGA GTTGCTGAGG CTGTAGTAGC TATGATTTTC 
CCTTTCCAGT GGCAGTGCCC TTACATCCCC CTGTGCCCAC TGTCTCTCGC GGATGTGCTT 
AGTGCACCTA TTCCCTTTAT TGTGGGAGTG GATTCTCGAT ACTTTGATTT GTATGACCCG 
CCTCAGGATG TTGTTTGCAT CGACTTGGAC ACAAGCATGG TTTACACGTC TGAAGACAAG 
AGGAATTTAA GCTGGAAACA GCTTCCCAAG AAACCAGTGA AAAACCTTAT TGGCGTCTTA 
AAAAAGCTTT ACCCACGACT CTCTTCAGTT CACCAAAAGT CTCCAGAAGA GTCTGCTGTG 
CTGATGGCCC CAATTGAGGC AGACTTCTCA TGGCAAAAGA AAACAACAGA TTTGGAGATG 
GAAATTCAAG AGGCATTTCT ACGGTTCATG GCATCCATAC TCAAAGGTTA TAGGGCTTAC 
TTGAAACCCA TAACTCAAGC ACCATCCAAC AAAGCTACTG CTGCAGATTC ACTGTTTGAC 
CGTCAAGGGT TTTTGAAAAG TAGGGATCGT GCACACATAA AATTCTACAC TTCACTGACA 
AAAACCCAGA TTTTCATCCG CTTCATTGAA GAGTGCAGCT TTGTCAGTGA TAAGGACACC 
AGCCTGGCCT TCTTTGATGA CTGCATTGAT AAGTTGTTTC CTGATAAAGG AGTGGAGAGA 
GGTGATAAGG GGGATCTGGA GTTTCCAGAA GACACTCGTC TGATTGAGCT AGATGACTCA 
CAGAAGAGCG AGCACACTGT GTTTATAATG CCACCTGAGC CTCTGCCGGA AGATGGACCT 
GACCCTCACC CAAGATATAG CTACAAAAAC TTTCCAAGAC TAGACTACGC TCTTTTTGAC 
CGAATACGTG ATCAATATCT TACTTTCAAT GGGAATACTA AGAGTAGCAG AGCAGCAAGC 
AGCCCCACAT TTATTGCAAA AAGGACAAAA CAGGAAATCA AAACGGCTCA CAAGTTAGCT 
AGAAAATATT TTAGCAACGA TCCTGCTTGG GGCAAATGTC TCTTCAGCCA GTGCTTCAGT 
TTGTGGTTTA TTTGCCTGCC TGTTTATGTT AAGGTATCAC ATTCAAAAGC ACGTGCACTT 
CAGAAGGCTT ATGATGTGCT GGTTAAGATG AGGAAAACTG ATGTAGATCC ATTGGATGAG 
GTGTGTTACA GAGTGGTAAT GCAACTGTGT GGCATTTGGG AGAACCCTGC TCTGGCAGTG 
AGAGTATTAT TTGAGATGAA AAACGCTGGG ATACAGCCAA ATGCAATTAC CTATGGTTAC 
TATAATAAGG TTGTGTTGGT CAGCCCATGG CCAAGCAGTA ATCGCAGTGG AAACTTCCTA 
TGGACAAAAG TGCGCAATGT GGTGTTTGGT TTGGCTCAGT TCCGAAGGGC AACGAAGAAG 
CCACAGACAT CTTCCGAGTC CTCTTCGGCA TCAGCGCTTC CAAGTGCTGC ACATGCAAAT 
GATGGCAATG CTGTGAGCCG ACAAAAAGTG GATAATTATA ACGATTCTAA CTCTGGGGAG 
CACACCCTTT TCGTGCGCGA TCTAATCAGA ACTGATTCCA CTGATAACCA TTCTAGCACA 
GAGAGTCCAT CTAAAAATGG CTCCAAAGAC AAGGTCGAGA CAGATGATGC TCAGAGTGGT 
TCTGATCAGA AAAACAAAGC AGAAACAGAC GCTAAAGCAA AGCAGAAACA CACACCAGAC 
TGTGATGAGC CTTCACTTTC CTCTATGGAT CCAACAGTAT CTAGTGAAGG ACATCCAGAA 
AAATTACATT CAACCTCTGA GGTTCCAAAT CCTCCTGAAT CACCTCCTTG TGCAAGTAGC 
ATTGTTAAAG TACCTTCTGG AATTTTTGAC AGCACAGGAA GGAAAAGCAG TACCGGAGGT 
GGAACTGGAC CATTATCCCC GGTTTCAGAT GTTGGGGAAG ACCATGTGTT TTCCAGCGAA 
GGTCAAAACA AGAAGTCCAG TGAGAGAGCT CAAAAGAGGC AGAAAATGTT TGCTGAGCGA 
AGCTGTAGTT TCAGCGCAGA GAGCCGAGCT GGTATGCTAC TAAAGAAAGG CAGTCTTGAC 
CTAACCACTA ATGAAATTGC CATAAAGATG GGAGCGGATG CAAAAATACT TACTGCAGCT 
CTTTCAAAGA CACCACCACC CCAGCATGCT AAGAATGAAT TCTGCTTTGA AAGTATAGGT 
GAACAGTCTG TCTGTCAGAC ATCTGATAGC ACAGCTTGTG AACAAGATGA AAAACCTGAC 
ACAGAGACTG AGGAGCTAAC AGCCTCACGA CTGCAGGGGC CATCTGATCA GTTAGATACG 
GTGAAAGAGG AAAGCACAGT GGATGCTGGG GACTCTGTTA TAGGCACAGT GAAAGACAGC 
GTTGCTCTCA GGGATACTCC TAAACTTATA AGTGAACTTA ATAAAAGGCA TAGTTTTTAT 
GGCCTCACTA AATCCGTAGA AAGAGAAGGG GTAGAAAAGG GTCTTGACCC TCTATCTTTA 
ATGGCTTCAC AGACAGTGGA GAAAGAGCCA TCACCATCTG AGGAAAAGCA CATTTCCCCT 
GTTGTGGCAC GTAATCTGGC CGATGAGATT GAAAGTTACA TGAACCTGAA AAGTCCTCTT 
GGCTCCAAGT CTTCAAGTAT GGAGCTAGGA GGACAGGAGT GCAATGCAAG GGAATCTGGG 
AACCACAGCC AAGCTGGCCT GGAGCGGCGC TCTAGCTTAC CCGTAGACTC TTTTCCTCCA 
TCAGAAGTGC AGAGTGAAAA CCAGAAGAAC TCAGCTGTAT CCAGATCCAA GACTTTTACT 
AGCAGTCCAA AGCATCACCT ACGCAACCAA AAGGAACGAT CAGTTTCACT CACTGCCCTT 
GTCCGCAGCT CCCCACATGG CTCTTTGGGT TCGATGGTTA ACTCCTTTCC TGGGCTAAAA 
TTTGACAACC TTCTGACAGG ACCTAAAATG GATGTGCTGA AATCAGGCAT GAAGCAGGCA 
GCCACTGTAG CAAGTAAAGT TTGGGGGGCT GTTGCATCCG CTTATAGCTA CTCTGATGAT 
GAGGAGGAAA GTCAAAGTCA GAGAGGTCAG TCTAACTTTC CTGTAAGACC TCAAGAGTCT 
GGTGTTGGTG ACAATCTTTC CTTCAAGATG CCTTCAAATG GACTTTCTGG CAATGAGCTG 
ACACAGAGCA ATACCAGCTT AGGCAGCAGC AGCAGTGGAG ACACAAGCAA GCACAGCTAC 
TTAAATGAAT CTGTACAGGG GAAAGCATCA AAGGTTCTAG AGTCTGAAAA ATCAGAGCAA 
GGATCCTCTC ACAGCACCAG CTTGTCAAGC ATATTTCAGA ACTGGGCAAT GGAGGTCTTG 
ATGTCTAGTT GCTCTCAGTG CCGAGGTTGT GATGCACTTG TGTATGATGA AGAGATAATG 
GCTGGATGGA CTTTTGATGA CTCAAACTTA AATACTACTT GTCCCTTTTG TAAAAGACCC 
TTTTTACCAC TTCTTAATAT TGAGTTTAAA GACCTTCGTG GTACTGCAAG CTTTTTTCTT 
AAGCCAAGTA ACTCAGGAGA CAGTCTTCAT GGCAGTAACC TTCGGGTAAT GGCAGATTCG 
TCAGAGCGTA AACTGCAATC AGAATCTACA GCCAGTGACC TGATCAGCTT TGGGGAATCT 
TCCACTACTC CCCAAACCAC AGCAGAGGAA AGTAATACAG ACACTCAGCA GAGTGACCAG 
GCTGAAGAAG ATGGAACCAT GAAGTCTTCT GAGACAAACA ATGCACCAAA GCGAGGAGCG 
TCACTGACTA GGAGTCACAG CGTAGGAGGG CCTCTACAAA ACATTGACTT ATCCCAAAGG 
CCTTCTCATG GAATTTCCAC TGTCAGTCTT CCCAACAGCC TGCAAGAAGC TGTGGATCCT 
TTGGAAAAGC GGCACAACCC TCCTCCTGTC TCCGTCCCCT ACCTTAGTCC CCTTGTCCTA 
CGCAAGGAGC TGGAATCTTT GCTGGAGAAT GAGGGCGACC ATGTCATCTA CACATCCACT 
TTTATTAACC AGCATCCTAT TATCTTCTGG AACCTGGTTT GGTTCTTTAG ACGTTTGGAC 
CTGCCCAGCA ATTTGCCAGG GCTCATCCTC TCATCTGAGC ACTGCAATGG AGGGGTGCAG 
CTCCCGCTAT CATCTCTATC TCAGGACAGC AAGCTAGTCT ATATACAACT GTTGTGGGAC 
AACATAAACC TTCACCAGGA GCCCGGGGAG CCCCTTTACA GCAAATTTAG AGCCCTGGAC 
AATATCAAGA AGAAGGCTTC TCCTATCCCA TATGATCAAC AAAGCACCAA CAATTTACTG 
GAAAGCATTA CGCTTAGTAT CCAGCATAAT GACGTCCTTA GGCCTATCAG CCTGCTCCTG 
CAGAAATGCA GCACAACTGG AAAGAGGCAA AGGAGCTTGT ATAGAGAAAT ACTGTACCTT 
TCTTTGGTGT CTCTTGGAAG AGAAAATATC GACATAGAGG CCTTTGACAA TGAATATAGA 
ATCGCTTATG AAAAGCTCCC TCTTGAGATG CAGCAAAAAA TGCAGAAAAT TGATCAGCCT 
CCAAGCAAGA AGGTGGAATG CTGCAGGAGT ATCTTTGGAG CGCCCCTAAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd4c ( XM_004910863.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910920.1 Xenopus tropicalis DENN domain containing 4C (dennd4c), transcript variant X2, mRNA.

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CloneID	OXa04068
Clone ID Related Accession (Same CDS sequence)	XM_004910862.4 , XM_004910863.3 , XM_004910863.4 , XM_004910862.3 , XM_002935667.4 , XM_002935667.5
Accession Version	XM_004910863.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DENN domain-containing protein 4C
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910863.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG ACAAAGGGCC TCGGGTAGCA GACTACTTTG TTGTGGCAGG ACTGACAGAC 
ACCTTCAAGC TATTAGATCA TGACATGGGT CGGATAGAAA CTAAGACATT AGGTCCCAAA 
GCTCCAATCA CTGATATTGC TGTTATCAAC AAGACAGCTG GAGAGACCGT GCCAGAGGGC 
TACACATGTA TTGAAATCAC ACAAAGTGGG TTGCAGGCAA ATCTGAACCA TGGAAGCTTA 
AAGAGTCCAG AAATGTTTAT CTGTTACAGA CGAGAAAAAG GGAAACCTCC ACTAACAGAC 
ATTGGTGTGT TATATGAAGG AAAAGAACAC CTATTGAAAG GCTGTGAGGT TATCCAGGCT 
ACACCATATG GACGCTGCGC AAATGTCAAT AACAGCTCGG GTACCTCCAT GAGGATATTT 
CTAACTTACC GACGATCTTT ACCTGTTCGC CTTCAGAACT CCTTGGCAGT AACTGACATA 
TGTATAATCA TCAGTAATAA AGGAGAGACA CCACCCCATA CCTTCTGTAA GGTGGACAAA 
AACCTCAACT GTGGAATGTG GGGATCCAAT GTTTTCCTGT GTTATAAGAA GTCTGTCCCT 
GCATCTAACT CAATTCCTTA TAAAGCTGGT TTGATTTTCA GATATCCAGA GGAGGATAAC 
GAGTCATTTC CTCTATCAGA GTCCGTGCCA CTCTTTTGCC TTCCCATGGG AGCCACTATT 
GAATGCTGGG ACCCTCAAAT CCGACATCCT CTCCCTGTTT TCTCTACTTT TGTATTGACA 
GGTTCCACAG CTGAAAAGGT TTATGGTGCT GCTATTCAGT TCTATGAGAC TTACCCACGT 
GAAATGTTGT CTGAGAAGCA GATGATTCAG TTAGGACTGA TCACAGCTGT GGAGAAGAAA 
GTGCTCACCT ACAAAAATAT TAACTCCAAG AAGTGCATAT GTTTGCTTTC TCACTGGCCT 
TTCTTTGAGA CCTTCCGCAA GTTTCTTATG TTCATCTACA AGCTGTCTGT GTCTGGCCCT 
CATACCCTTC CTATTGAAAA GCACATATCG CACTTTATGC ACAATGTTCC CTTCCCATCA 
CCACAGAGGC CAAGAATTCT TGTTCAGCTC TCTGCTCATG ATGCCTTAGT TCTGTCCCAG 
CCAGTAGCAA CACCAATGCC TCTAAGTGGA GCAAATTTTA GTACACTGCT TATGAATCTT 
GGACCAGAAA ACTGTGCTTC TTTGCTGTTG TTCGTACTGC TAGAATGCAA GATTCTCCTT 
CACTCCCTCA GGCCAGCAGT GCTGACTGGA GTTGCTGAGG CTGTAGTAGC TATGATTTTC 
CCTTTCCAGT GGCAGTGCCC TTACATCCCC CTGTGCCCAC TGTCTCTCGC GGATGTGCTT 
AGTGCACCTA TTCCCTTTAT TGTGGGAGTG GATTCTCGAT ACTTTGATTT GTATGACCCG 
CCTCAGGATG TTGTTTGCAT CGACTTGGAC ACAAGCATGG TTTACACGTC TGAAGACAAG 
AGGAATTTAA GCTGGAAACA GCTTCCCAAG AAACCAGTGA AAAACCTTAT TGGCGTCTTA 
AAAAAGCTTT ACCCACGACT CTCTTCAGTT CACCAAAAGT CTCCAGAAGA GTCTGCTGTG 
CTGATGGCCC CAATTGAGGC AGACTTCTCA TGGCAAAAGA AAACAACAGA TTTGGAGATG 
GAAATTCAAG AGGCATTTCT ACGGTTCATG GCATCCATAC TCAAAGGTTA TAGGGCTTAC 
TTGAAACCCA TAACTCAAGC ACCATCCAAC AAAGCTACTG CTGCAGATTC ACTGTTTGAC 
CGTCAAGGGT TTTTGAAAAG TAGGGATCGT GCACACATAA AATTCTACAC TTCACTGACA 
AAAACCCAGA TTTTCATCCG CTTCATTGAA GAGTGCAGCT TTGTCAGTGA TAAGGACACC 
AGCCTGGCCT TCTTTGATGA CTGCATTGAT AAGTTGTTTC CTGATAAAGG AGTGGAGAGA 
GGTGATAAGG GGGATCTGGA GTTTCCAGAA GACACTCGTC TGATTGAGCT AGATGACTCA 
CAGAAGAGCG AGCACACTGT GTTTATAATG CCACCTGAGC CTCTGCCGGA AGATGGACCT 
GACCCTCACC CAAGATATAG CTACAAAAAC TTTCCAAGAC TAGACTACGC TCTTTTTGAC 
CGAATACGTG ATCAATATCT TACTTTCAAT GGGAATACTA AGAGTAGCAG AGCAGCAAGC 
AGCCCCACAT TTATTGCAAA AAGGACAAAA CAGGAAATCA AAACGGCTCA CAAGTTAGCT 
AGAAAATATT TTAGCAACGA TCCTGCTTGG GGCAAATGTC TCTTCAGCCA GTGCTTCAGT 
TTGTGGTTTA TTTGCCTGCC TGTTTATGTT AAGGTATCAC ATTCAAAAGC ACGTGCACTT 
CAGAAGGCTT ATGATGTGCT GGTTAAGATG AGGAAAACTG ATGTAGATCC ATTGGATGAG 
GTGTGTTACA GAGTGGTAAT GCAACTGTGT GGCATTTGGG AGAACCCTGC TCTGGCAGTG 
AGAGTATTAT TTGAGATGAA AAACGCTGGG ATACAGCCAA ATGCAATTAC CTATGGTTAC 
TATAATAAGG TTGTGTTGGT CAGCCCATGG CCAAGCAGTA ATCGCAGTGG AAACTTCCTA 
TGGACAAAAG TGCGCAATGT GGTGTTTGGT TTGGCTCAGT TCCGAAGGGC AACGAAGAAG 
CCACAGACAT CTTCCGAGTC CTCTTCGGCA TCAGCGCTTC CAAGTGCTGC ACATGCAAAT 
GATGGCAATG CTGTGAGCCG ACAAAAAGTG GATAATTATA ACGATTCTAA CTCTGGGGAG 
CACACCCTTT TCGTGCGCGA TCTAATCAGA ACTGATTCCA CTGATAACCA TTCTAGCACA 
GAGAGTCCAT CTAAAAATGG CTCCAAAGAC AAGGTCGAGA CAGATGATGC TCAGAGTGGT 
TCTGATCAGA AAAACAAAGC AGAAACAGAC GCTAAAGCAA AGCAGAAACA CACACCAGAC 
TGTGATGAGC CTTCACTTTC CTCTATGGAT CCAACAGTAT CTAGTGAAGG ACATCCAGAA 
AAATTACATT CAACCTCTGA GGTTCCAAAT CCTCCTGAAT CACCTCCTTG TGCAAGTAGC 
ATTGTTAAAG TACCTTCTGG AATTTTTGAC AGCACAGGAA GGAAAAGCAG TACCGGAGGT 
GGAACTGGAC CATTATCCCC GGTTTCAGAT GTTGGGGAAG ACCATGTGTT TTCCAGCGAA 
GGTCAAAACA AGAAGTCCAG TGAGAGAGCT CAAAAGAGGC AGAAAATGTT TGCTGAGCGA 
AGCTGTAGTT TCAGCGCAGA GAGCCGAGCT GGTATGCTAC TAAAGAAAGG CAGTCTTGAC 
CTAACCACTA ATGAAATTGC CATAAAGATG GGAGCGGATG CAAAAATACT TACTGCAGCT 
CTTTCAAAGA CACCACCACC CCAGCATGCT AAGAATGAAT TCTGCTTTGA AAGTATAGGT 
GAACAGTCTG TCTGTCAGAC ATCTGATAGC ACAGCTTGTG AACAAGATGA AAAACCTGAC 
ACAGAGACTG AGGAGCTAAC AGCCTCACGA CTGCAGGGGC CATCTGATCA GTTAGATACG 
GTGAAAGAGG AAAGCACAGT GGATGCTGGG GACTCTGTTA TAGGCACAGT GAAAGACAGC 
GTTGCTCTCA GGGATACTCC TAAACTTATA AGTGAACTTA ATAAAAGGCA TAGTTTTTAT 
GGCCTCACTA AATCCGTAGA AAGAGAAGGG GTAGAAAAGG GTCTTGACCC TCTATCTTTA 
ATGGCTTCAC AGACAGTGGA GAAAGAGCCA TCACCATCTG AGGAAAAGCA CATTTCCCCT 
GTTGTGGCAC GTAATCTGGC CGATGAGATT GAAAGTTACA TGAACCTGAA AAGTCCTCTT 
GGCTCCAAGT CTTCAAGTAT GGAGCTAGGA GGACAGGAGT GCAATGCAAG GGAATCTGGG 
AACCACAGCC AAGCTGGCCT GGAGCGGCGC TCTAGCTTAC CCGTAGACTC TTTTCCTCCA 
TCAGAAGTGC AGAGTGAAAA CCAGAAGAAC TCAGCTGTAT CCAGATCCAA GACTTTTACT 
AGCAGTCCAA AGCATCACCT ACGCAACCAA AAGGAACGAT CAGTTTCACT CACTGCCCTT 
GTCCGCAGCT CCCCACATGG CTCTTTGGGT TCGATGGTTA ACTCCTTTCC TGGGCTAAAA 
TTTGACAACC TTCTGACAGG ACCTAAAATG GATGTGCTGA AATCAGGCAT GAAGCAGGCA 
GCCACTGTAG CAAGTAAAGT TTGGGGGGCT GTTGCATCCG CTTATAGCTA CTCTGATGAT 
GAGGAGGAAA GTCAAAGTCA GAGAGGTCAG TCTAACTTTC CTGTAAGACC TCAAGAGTCT 
GGTGTTGGTG ACAATCTTTC CTTCAAGATG CCTTCAAATG GACTTTCTGG CAATGAGCTG 
ACACAGAGCA ATACCAGCTT AGGCAGCAGC AGCAGTGGAG ACACAAGCAA GCACAGCTAC 
TTAAATGAAT CTGTACAGGG GAAAGCATCA AAGGTTCTAG AGTCTGAAAA ATCAGAGCAA 
GGATCCTCTC ACAGCACCAG CTTGTCAAGC ATATTTCAGA ACTGGGCAAT GGAGGTCTTG 
ATGTCTAGTT GCTCTCAGTG CCGAGGTTGT GATGCACTTG TGTATGATGA AGAGATAATG 
GCTGGATGGA CTTTTGATGA CTCAAACTTA AATACTACTT GTCCCTTTTG TAAAAGACCC 
TTTTTACCAC TTCTTAATAT TGAGTTTAAA GACCTTCGTG GTACTGCAAG CTTTTTTCTT 
AAGCCAAGTA ACTCAGGAGA CAGTCTTCAT GGCAGTAACC TTCGGGTAAT GGCAGATTCG 
TCAGAGCGTA AACTGCAATC AGAATCTACA GCCAGTGACC TGATCAGCTT TGGGGAATCT 
TCCACTACTC CCCAAACCAC AGCAGAGGAA AGTAATACAG ACACTCAGCA GAGTGACCAG 
GCTGAAGAAG ATGGAACCAT GAAGTCTTCT GAGACAAACA ATGCACCAAA GCGAGGAGCG 
TCACTGACTA GGAGTCACAG CGTAGGAGGG CCTCTACAAA ACATTGACTT ATCCCAAAGG 
CCTTCTCATG GAATTTCCAC TGTCAGTCTT CCCAACAGCC TGCAAGAAGC TGTGGATCCT 
TTGGAAAAGC GGCACAACCC TCCTCCTGTC TCCGTCCCCT ACCTTAGTCC CCTTGTCCTA 
CGCAAGGAGC TGGAATCTTT GCTGGAGAAT GAGGGCGACC ATGTCATCTA CACATCCACT 
TTTATTAACC AGCATCCTAT TATCTTCTGG AACCTGGTTT GGTTCTTTAG ACGTTTGGAC 
CTGCCCAGCA ATTTGCCAGG GCTCATCCTC TCATCTGAGC ACTGCAATGG AGGGGTGCAG 
CTCCCGCTAT CATCTCTATC TCAGGACAGC AAGCTAGTCT ATATACAACT GTTGTGGGAC 
AACATAAACC TTCACCAGGA GCCCGGGGAG CCCCTTTACA GCAAATTTAG AGCCCTGGAC 
AATATCAAGA AGAAGGCTTC TCCTATCCCA TATGATCAAC AAAGCACCAA CAATTTACTG 
GAAAGCATTA CGCTTAGTAT CCAGCATAAT GACGTCCTTA GGCCTATCAG CCTGCTCCTG 
CAGAAATGCA GCACAACTGG AAAGAGGCAA AGGAGCTTGT ATAGAGAAAT ACTGTACCTT 
TCTTTGGTGT CTCTTGGAAG AGAAAATATC GACATAGAGG CCTTTGACAA TGAATATAGA 
ATCGCTTATG AAAAGCTCCC TCTTGAGATG CAGCAAAAAA TGCAGAAAAT TGATCAGCCT 
CCAAGCAAGA AGGTGGAATG CTGCAGGAGT ATCTTTGGAG CGCCCCTAAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910920.1
CDS	99..5972
Translation	MMEDKGPRVADYFVVAGLTDTFKLLDHDMGRIETKTLGPKAPITDIAVINKTAGETVPEGYTCIEITQSGLQANLNHGSLKSPEMFICYRREKGKPPLTDIGVLYEGKEHLLKGCEVIQATPYGRCANVNNSSGTSMRIFLTYRRSLPVRLQNSLAVTDICIIISNKGETPPHTFCKVDKNLNCGMWGSNVFLCYKKSVPASNSIPYKAGLIFRYPEEDNESFPLSESVPLFCLPMGATIECWDPQIRHPLPVFSTFVLTGSTAEKVYGAAIQFYETYPREMLSEKQMIQLGLITAVEKKVLTYKNINSKKCICLLSHWPFFETFRKFLMFIYKLSVSGPHTLPIEKHISHFMHNVPFPSPQRPRILVQLSAHDALVLSQPVATPMPLSGANFSTLLMNLGPENCASLLLFVLLECKILLHSLRPAVLTGVAEAVVAMIFPFQWQCPYIPLCPLSLADVLSAPIPFIVGVDSRYFDLYDPPQDVVCIDLDTSMVYTSEDKRNLSWKQLPKKPVKNLIGVLKKLYPRLSSVHQKSPEESAVLMAPIEADFSWQKKTTDLEMEIQEAFLRFMASILKGYRAYLKPITQAPSNKATAADSLFDRQGFLKSRDRAHIKFYTSLTKTQIFIRFIEECSFVSDKDTSLAFFDDCIDKLFPDKGVERGDKGDLEFPEDTRLIELDDSQKSEHTVFIMPPEPLPEDGPDPHPRYSYKNFPRLDYALFDRIRDQYLTFNGNTKSSRAASSPTFIAKRTKQEIKTAHKLARKYFSNDPAWGKCLFSQCFSLWFICLPVYVKVSHSKARALQKAYDVLVKMRKTDVDPLDEVCYRVVMQLCGIWENPALAVRVLFEMKNAGIQPNAITYGYYNKVVLVSPWPSSNRSGNFLWTKVRNVVFGLAQFRRATKKPQTSSESSSASALPSAAHANDGNAVSRQKVDNYNDSNSGEHTLFVRDLIRTDSTDNHSSTESPSKNGSKDKVETDDAQSGSDQKNKAETDAKAKQKHTPDCDEPSLSSMDPTVSSEGHPEKLHSTSEVPNPPESPPCASSIVKVPSGIFDSTGRKSSTGGGTGPLSPVSDVGEDHVFSSEGQNKKSSERAQKRQKMFAERSCSFSAESRAGMLLKKGSLDLTTNEIAIKMGADAKILTAALSKTPPPQHAKNEFCFESIGEQSVCQTSDSTACEQDEKPDTETEELTASRLQGPSDQLDTVKEESTVDAGDSVIGTVKDSVALRDTPKLISELNKRHSFYGLTKSVEREGVEKGLDPLSLMASQTVEKEPSPSEEKHISPVVARNLADEIESYMNLKSPLGSKSSSMELGGQECNARESGNHSQAGLERRSSLPVDSFPPSEVQSENQKNSAVSRSKTFTSSPKHHLRNQKERSVSLTALVRSSPHGSLGSMVNSFPGLKFDNLLTGPKMDVLKSGMKQAATVASKVWGAVASAYSYSDDEEESQSQRGQSNFPVRPQESGVGDNLSFKMPSNGLSGNELTQSNTSLGSSSSGDTSKHSYLNESVQGKASKVLESEKSEQGSSHSTSLSSIFQNWAMEVLMSSCSQCRGCDALVYDEEIMAGWTFDDSNLNTTCPFCKRPFLPLLNIEFKDLRGTASFFLKPSNSGDSLHGSNLRVMADSSERKLQSESTASDLISFGESSTTPQTTAEESNTDTQQSDQAEEDGTMKSSETNNAPKRGASLTRSHSVGGPLQNIDLSQRPSHGISTVSLPNSLQEAVDPLEKRHNPPPVSVPYLSPLVLRKELESLLENEGDHVIYTSTFINQHPIIFWNLVWFFRRLDLPSNLPGLILSSEHCNGGVQLPLSSLSQDSKLVYIQLLWDNINLHQEPGEPLYSKFRALDNIKKKASPIPYDQQSTNNLLESITLSIQHNDVLRPISLLLQKCSTTGKRQRSLYREILYLSLVSLGRENIDIEAFDNEYRIAYEKLPLEMQQKMQKIDQPPSKKVECCRSIFGAPLI

Target ORF information:

RefSeq Version	XM_004910863.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN domain containing 4C (dennd4c), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910863.4

ORF Insert Sequence:

ATGATGGAAG ACAAAGGGCC TCGGGTAGCA GACTACTTTG TTGTGGCAGG ACTGACAGAC 
ACCTTCAAGC TATTAGATCA TGACATGGGT CGGATAGAAA CTAAGACATT AGGTCCCAAA 
GCTCCAATCA CTGATATTGC TGTTATCAAC AAGACAGCTG GAGAGACCGT GCCAGAGGGC 
TACACATGTA TTGAAATCAC ACAAAGTGGG TTGCAGGCAA ATCTGAACCA TGGAAGCTTA 
AAGAGTCCAG AAATGTTTAT CTGTTACAGA CGAGAAAAAG GGAAACCTCC ACTAACAGAC 
ATTGGTGTGT TATATGAAGG AAAAGAACAC CTATTGAAAG GCTGTGAGGT TATCCAGGCT 
ACACCATATG GACGCTGCGC AAATGTCAAT AACAGCTCGG GTACCTCCAT GAGGATATTT 
CTAACTTACC GACGATCTTT ACCTGTTCGC CTTCAGAACT CCTTGGCAGT AACTGACATA 
TGTATAATCA TCAGTAATAA AGGAGAGACA CCACCCCATA CCTTCTGTAA GGTGGACAAA 
AACCTCAACT GTGGAATGTG GGGATCCAAT GTTTTCCTGT GTTATAAGAA GTCTGTCCCT 
GCATCTAACT CAATTCCTTA TAAAGCTGGT TTGATTTTCA GATATCCAGA GGAGGATAAC 
GAGTCATTTC CTCTATCAGA GTCCGTGCCA CTCTTTTGCC TTCCCATGGG AGCCACTATT 
GAATGCTGGG ACCCTCAAAT CCGACATCCT CTCCCTGTTT TCTCTACTTT TGTATTGACA 
GGTTCCACAG CTGAAAAGGT TTATGGTGCT GCTATTCAGT TCTATGAGAC TTACCCACGT 
GAAATGTTGT CTGAGAAGCA GATGATTCAG TTAGGACTGA TCACAGCTGT GGAGAAGAAA 
GTGCTCACCT ACAAAAATAT TAACTCCAAG AAGTGCATAT GTTTGCTTTC TCACTGGCCT 
TTCTTTGAGA CCTTCCGCAA GTTTCTTATG TTCATCTACA AGCTGTCTGT GTCTGGCCCT 
CATACCCTTC CTATTGAAAA GCACATATCG CACTTTATGC ACAATGTTCC CTTCCCATCA 
CCACAGAGGC CAAGAATTCT TGTTCAGCTC TCTGCTCATG ATGCCTTAGT TCTGTCCCAG 
CCAGTAGCAA CACCAATGCC TCTAAGTGGA GCAAATTTTA GTACACTGCT TATGAATCTT 
GGACCAGAAA ACTGTGCTTC TTTGCTGTTG TTCGTACTGC TAGAATGCAA GATTCTCCTT 
CACTCCCTCA GGCCAGCAGT GCTGACTGGA GTTGCTGAGG CTGTAGTAGC TATGATTTTC 
CCTTTCCAGT GGCAGTGCCC TTACATCCCC CTGTGCCCAC TGTCTCTCGC GGATGTGCTT 
AGTGCACCTA TTCCCTTTAT TGTGGGAGTG GATTCTCGAT ACTTTGATTT GTATGACCCG 
CCTCAGGATG TTGTTTGCAT CGACTTGGAC ACAAGCATGG TTTACACGTC TGAAGACAAG 
AGGAATTTAA GCTGGAAACA GCTTCCCAAG AAACCAGTGA AAAACCTTAT TGGCGTCTTA 
AAAAAGCTTT ACCCACGACT CTCTTCAGTT CACCAAAAGT CTCCAGAAGA GTCTGCTGTG 
CTGATGGCCC CAATTGAGGC AGACTTCTCA TGGCAAAAGA AAACAACAGA TTTGGAGATG 
GAAATTCAAG AGGCATTTCT ACGGTTCATG GCATCCATAC TCAAAGGTTA TAGGGCTTAC 
TTGAAACCCA TAACTCAAGC ACCATCCAAC AAAGCTACTG CTGCAGATTC ACTGTTTGAC 
CGTCAAGGGT TTTTGAAAAG TAGGGATCGT GCACACATAA AATTCTACAC TTCACTGACA 
AAAACCCAGA TTTTCATCCG CTTCATTGAA GAGTGCAGCT TTGTCAGTGA TAAGGACACC 
AGCCTGGCCT TCTTTGATGA CTGCATTGAT AAGTTGTTTC CTGATAAAGG AGTGGAGAGA 
GGTGATAAGG GGGATCTGGA GTTTCCAGAA GACACTCGTC TGATTGAGCT AGATGACTCA 
CAGAAGAGCG AGCACACTGT GTTTATAATG CCACCTGAGC CTCTGCCGGA AGATGGACCT 
GACCCTCACC CAAGATATAG CTACAAAAAC TTTCCAAGAC TAGACTACGC TCTTTTTGAC 
CGAATACGTG ATCAATATCT TACTTTCAAT GGGAATACTA AGAGTAGCAG AGCAGCAAGC 
AGCCCCACAT TTATTGCAAA AAGGACAAAA CAGGAAATCA AAACGGCTCA CAAGTTAGCT 
AGAAAATATT TTAGCAACGA TCCTGCTTGG GGCAAATGTC TCTTCAGCCA GTGCTTCAGT 
TTGTGGTTTA TTTGCCTGCC TGTTTATGTT AAGGTATCAC ATTCAAAAGC ACGTGCACTT 
CAGAAGGCTT ATGATGTGCT GGTTAAGATG AGGAAAACTG ATGTAGATCC ATTGGATGAG 
GTGTGTTACA GAGTGGTAAT GCAACTGTGT GGCATTTGGG AGAACCCTGC TCTGGCAGTG 
AGAGTATTAT TTGAGATGAA AAACGCTGGG ATACAGCCAA ATGCAATTAC CTATGGTTAC 
TATAATAAGG TTGTGTTGGT CAGCCCATGG CCAAGCAGTA ATCGCAGTGG AAACTTCCTA 
TGGACAAAAG TGCGCAATGT GGTGTTTGGT TTGGCTCAGT TCCGAAGGGC AACGAAGAAG 
CCACAGACAT CTTCCGAGTC CTCTTCGGCA TCAGCGCTTC CAAGTGCTGC ACATGCAAAT 
GATGGCAATG CTGTGAGCCG ACAAAAAGTG GATAATTATA ACGATTCTAA CTCTGGGGAG 
CACACCCTTT TCGTGCGCGA TCTAATCAGA ACTGATTCCA CTGATAACCA TTCTAGCACA 
GAGAGTCCAT CTAAAAATGG CTCCAAAGAC AAGGTCGAGA CAGATGATGC TCAGAGTGGT 
TCTGATCAGA AAAACAAAGC AGAAACAGAC GCTAAAGCAA AGCAGAAACA CACACCAGAC 
TGTGATGAGC CTTCACTTTC CTCTATGGAT CCAACAGTAT CTAGTGAAGG ACATCCAGAA 
AAATTACATT CAACCTCTGA GGTTCCAAAT CCTCCTGAAT CACCTCCTTG TGCAAGTAGC 
ATTGTTAAAG TACCTTCTGG AATTTTTGAC AGCACAGGAA GGAAAAGCAG TACCGGAGGT 
GGAACTGGAC CATTATCCCC GGTTTCAGAT GTTGGGGAAG ACCATGTGTT TTCCAGCGAA 
GGTCAAAACA AGAAGTCCAG TGAGAGAGCT CAAAAGAGGC AGAAAATGTT TGCTGAGCGA 
AGCTGTAGTT TCAGCGCAGA GAGCCGAGCT GGTATGCTAC TAAAGAAAGG CAGTCTTGAC 
CTAACCACTA ATGAAATTGC CATAAAGATG GGAGCGGATG CAAAAATACT TACTGCAGCT 
CTTTCAAAGA CACCACCACC CCAGCATGCT AAGAATGAAT TCTGCTTTGA AAGTATAGGT 
GAACAGTCTG TCTGTCAGAC ATCTGATAGC ACAGCTTGTG AACAAGATGA AAAACCTGAC 
ACAGAGACTG AGGAGCTAAC AGCCTCACGA CTGCAGGGGC CATCTGATCA GTTAGATACG 
GTGAAAGAGG AAAGCACAGT GGATGCTGGG GACTCTGTTA TAGGCACAGT GAAAGACAGC 
GTTGCTCTCA GGGATACTCC TAAACTTATA AGTGAACTTA ATAAAAGGCA TAGTTTTTAT 
GGCCTCACTA AATCCGTAGA AAGAGAAGGG GTAGAAAAGG GTCTTGACCC TCTATCTTTA 
ATGGCTTCAC AGACAGTGGA GAAAGAGCCA TCACCATCTG AGGAAAAGCA CATTTCCCCT 
GTTGTGGCAC GTAATCTGGC CGATGAGATT GAAAGTTACA TGAACCTGAA AAGTCCTCTT 
GGCTCCAAGT CTTCAAGTAT GGAGCTAGGA GGACAGGAGT GCAATGCAAG GGAATCTGGG 
AACCACAGCC AAGCTGGCCT GGAGCGGCGC TCTAGCTTAC CCGTAGACTC TTTTCCTCCA 
TCAGAAGTGC AGAGTGAAAA CCAGAAGAAC TCAGCTGTAT CCAGATCCAA GACTTTTACT 
AGCAGTCCAA AGCATCACCT ACGCAACCAA AAGGAACGAT CAGTTTCACT CACTGCCCTT 
GTCCGCAGCT CCCCACATGG CTCTTTGGGT TCGATGGTTA ACTCCTTTCC TGGGCTAAAA 
TTTGACAACC TTCTGACAGG ACCTAAAATG GATGTGCTGA AATCAGGCAT GAAGCAGGCA 
GCCACTGTAG CAAGTAAAGT TTGGGGGGCT GTTGCATCCG CTTATAGCTA CTCTGATGAT 
GAGGAGGAAA GTCAAAGTCA GAGAGGTCAG TCTAACTTTC CTGTAAGACC TCAAGAGTCT 
GGTGTTGGTG ACAATCTTTC CTTCAAGATG CCTTCAAATG GACTTTCTGG CAATGAGCTG 
ACACAGAGCA ATACCAGCTT AGGCAGCAGC AGCAGTGGAG ACACAAGCAA GCACAGCTAC 
TTAAATGAAT CTGTACAGGG GAAAGCATCA AAGGTTCTAG AGTCTGAAAA ATCAGAGCAA 
GGATCCTCTC ACAGCACCAG CTTGTCAAGC ATATTTCAGA ACTGGGCAAT GGAGGTCTTG 
ATGTCTAGTT GCTCTCAGTG CCGAGGTTGT GATGCACTTG TGTATGATGA AGAGATAATG 
GCTGGATGGA CTTTTGATGA CTCAAACTTA AATACTACTT GTCCCTTTTG TAAAAGACCC 
TTTTTACCAC TTCTTAATAT TGAGTTTAAA GACCTTCGTG GTACTGCAAG CTTTTTTCTT 
AAGCCAAGTA ACTCAGGAGA CAGTCTTCAT GGCAGTAACC TTCGGGTAAT GGCAGATTCG 
TCAGAGCGTA AACTGCAATC AGAATCTACA GCCAGTGACC TGATCAGCTT TGGGGAATCT 
TCCACTACTC CCCAAACCAC AGCAGAGGAA AGTAATACAG ACACTCAGCA GAGTGACCAG 
GCTGAAGAAG ATGGAACCAT GAAGTCTTCT GAGACAAACA ATGCACCAAA GCGAGGAGCG 
TCACTGACTA GGAGTCACAG CGTAGGAGGG CCTCTACAAA ACATTGACTT ATCCCAAAGG 
CCTTCTCATG GAATTTCCAC TGTCAGTCTT CCCAACAGCC TGCAAGAAGC TGTGGATCCT 
TTGGAAAAGC GGCACAACCC TCCTCCTGTC TCCGTCCCCT ACCTTAGTCC CCTTGTCCTA 
CGCAAGGAGC TGGAATCTTT GCTGGAGAAT GAGGGCGACC ATGTCATCTA CACATCCACT 
TTTATTAACC AGCATCCTAT TATCTTCTGG AACCTGGTTT GGTTCTTTAG ACGTTTGGAC 
CTGCCCAGCA ATTTGCCAGG GCTCATCCTC TCATCTGAGC ACTGCAATGG AGGGGTGCAG 
CTCCCGCTAT CATCTCTATC TCAGGACAGC AAGCTAGTCT ATATACAACT GTTGTGGGAC 
AACATAAACC TTCACCAGGA GCCCGGGGAG CCCCTTTACA GCAAATTTAG AGCCCTGGAC 
AATATCAAGA AGAAGGCTTC TCCTATCCCA TATGATCAAC AAAGCACCAA CAATTTACTG 
GAAAGCATTA CGCTTAGTAT CCAGCATAAT GACGTCCTTA GGCCTATCAG CCTGCTCCTG 
CAGAAATGCA GCACAACTGG AAAGAGGCAA AGGAGCTTGT ATAGAGAAAT ACTGTACCTT 
TCTTTGGTGT CTCTTGGAAG AGAAAATATC GACATAGAGG CCTTTGACAA TGAATATAGA 
ATCGCTTATG AAAAGCTCCC TCTTGAGATG CAGCAAAAAA TGCAGAAAAT TGATCAGCCT 
CCAAGCAAGA AGGTGGAATG CTGCAGGAGT ATCTTTGGAG CGCCCCTAAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd4c ( XM_002935667.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935713.2 Xenopus tropicalis DENN domain containing 4C (dennd4c), transcript variant X3, mRNA.

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CloneID	OXa04068
Clone ID Related Accession (Same CDS sequence)	XM_004910862.4 , XM_004910863.3 , XM_004910863.4 , XM_004910862.3 , XM_002935667.4 , XM_002935667.5
Accession Version	XM_002935667.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DENN domain-containing protein 4C
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935667.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG ACAAAGGGCC TCGGGTAGCA GACTACTTTG TTGTGGCAGG ACTGACAGAC 
ACCTTCAAGC TATTAGATCA TGACATGGGT CGGATAGAAA CTAAGACATT AGGTCCCAAA 
GCTCCAATCA CTGATATTGC TGTTATCAAC AAGACAGCTG GAGAGACCGT GCCAGAGGGC 
TACACATGTA TTGAAATCAC ACAAAGTGGG TTGCAGGCAA ATCTGAACCA TGGAAGCTTA 
AAGAGTCCAG AAATGTTTAT CTGTTACAGA CGAGAAAAAG GGAAACCTCC ACTAACAGAC 
ATTGGTGTGT TATATGAAGG AAAAGAACAC CTATTGAAAG GCTGTGAGGT TATCCAGGCT 
ACACCATATG GACGCTGCGC AAATGTCAAT AACAGCTCGG GTACCTCCAT GAGGATATTT 
CTAACTTACC GACGATCTTT ACCTGTTCGC CTTCAGAACT CCTTGGCAGT AACTGACATA 
TGTATAATCA TCAGTAATAA AGGAGAGACA CCACCCCATA CCTTCTGTAA GGTGGACAAA 
AACCTCAACT GTGGAATGTG GGGATCCAAT GTTTTCCTGT GTTATAAGAA GTCTGTCCCT 
GCATCTAACT CAATTCCTTA TAAAGCTGGT TTGATTTTCA GATATCCAGA GGAGGATAAC 
GAGTCATTTC CTCTATCAGA GTCCGTGCCA CTCTTTTGCC TTCCCATGGG AGCCACTATT 
GAATGCTGGG ACCCTCAAAT CCGACATCCT CTCCCTGTTT TCTCTACTTT TGTATTGACA 
GGTTCCACAG CTGAAAAGGT TTATGGTGCT GCTATTCAGT TCTATGAGAC TTACCCACGT 
GAAATGTTGT CTGAGAAGCA GATGATTCAG TTAGGACTGA TCACAGCTGT GGAGAAGAAA 
GTGCTCACCT ACAAAAATAT TAACTCCAAG AAGTGCATAT GTTTGCTTTC TCACTGGCCT 
TTCTTTGAGA CCTTCCGCAA GTTTCTTATG TTCATCTACA AGCTGTCTGT GTCTGGCCCT 
CATACCCTTC CTATTGAAAA GCACATATCG CACTTTATGC ACAATGTTCC CTTCCCATCA 
CCACAGAGGC CAAGAATTCT TGTTCAGCTC TCTGCTCATG ATGCCTTAGT TCTGTCCCAG 
CCAGTAGCAA CACCAATGCC TCTAAGTGGA GCAAATTTTA GTACACTGCT TATGAATCTT 
GGACCAGAAA ACTGTGCTTC TTTGCTGTTG TTCGTACTGC TAGAATGCAA GATTCTCCTT 
CACTCCCTCA GGCCAGCAGT GCTGACTGGA GTTGCTGAGG CTGTAGTAGC TATGATTTTC 
CCTTTCCAGT GGCAGTGCCC TTACATCCCC CTGTGCCCAC TGTCTCTCGC GGATGTGCTT 
AGTGCACCTA TTCCCTTTAT TGTGGGAGTG GATTCTCGAT ACTTTGATTT GTATGACCCG 
CCTCAGGATG TTGTTTGCAT CGACTTGGAC ACAAGCATGG TTTACACGTC TGAAGACAAG 
AGGAATTTAA GCTGGAAACA GCTTCCCAAG AAACCAGTGA AAAACCTTAT TGGCGTCTTA 
AAAAAGCTTT ACCCACGACT CTCTTCAGTT CACCAAAAGT CTCCAGAAGA GTCTGCTGTG 
CTGATGGCCC CAATTGAGGC AGACTTCTCA TGGCAAAAGA AAACAACAGA TTTGGAGATG 
GAAATTCAAG AGGCATTTCT ACGGTTCATG GCATCCATAC TCAAAGGTTA TAGGGCTTAC 
TTGAAACCCA TAACTCAAGC ACCATCCAAC AAAGCTACTG CTGCAGATTC ACTGTTTGAC 
CGTCAAGGGT TTTTGAAAAG TAGGGATCGT GCACACATAA AATTCTACAC TTCACTGACA 
AAAACCCAGA TTTTCATCCG CTTCATTGAA GAGTGCAGCT TTGTCAGTGA TAAGGACACC 
AGCCTGGCCT TCTTTGATGA CTGCATTGAT AAGTTGTTTC CTGATAAAGG AGTGGAGAGA 
GGTGATAAGG GGGATCTGGA GTTTCCAGAA GACACTCGTC TGATTGAGCT AGATGACTCA 
CAGAAGAGCG AGCACACTGT GTTTATAATG CCACCTGAGC CTCTGCCGGA AGATGGACCT 
GACCCTCACC CAAGATATAG CTACAAAAAC TTTCCAAGAC TAGACTACGC TCTTTTTGAC 
CGAATACGTG ATCAATATCT TACTTTCAAT GGGAATACTA AGAGTAGCAG AGCAGCAAGC 
AGCCCCACAT TTATTGCAAA AAGGACAAAA CAGGAAATCA AAACGGCTCA CAAGTTAGCT 
AGAAAATATT TTAGCAACGA TCCTGCTTGG GGCAAATGTC TCTTCAGCCA GTGCTTCAGT 
TTGTGGTTTA TTTGCCTGCC TGTTTATGTT AAGGTATCAC ATTCAAAAGC ACGTGCACTT 
CAGAAGGCTT ATGATGTGCT GGTTAAGATG AGGAAAACTG ATGTAGATCC ATTGGATGAG 
GTGTGTTACA GAGTGGTAAT GCAACTGTGT GGCATTTGGG AGAACCCTGC TCTGGCAGTG 
AGAGTATTAT TTGAGATGAA AAACGCTGGG ATACAGCCAA ATGCAATTAC CTATGGTTAC 
TATAATAAGG TTGTGTTGGT CAGCCCATGG CCAAGCAGTA ATCGCAGTGG AAACTTCCTA 
TGGACAAAAG TGCGCAATGT GGTGTTTGGT TTGGCTCAGT TCCGAAGGGC AACGAAGAAG 
CCACAGACAT CTTCCGAGTC CTCTTCGGCA TCAGCGCTTC CAAGTGCTGC ACATGCAAAT 
GATGGCAATG CTGTGAGCCG ACAAAAAGTG GATAATTATA ACGATTCTAA CTCTGGGGAG 
CACACCCTTT TCGTGCGCGA TCTAATCAGA ACTGATTCCA CTGATAACCA TTCTAGCACA 
GAGAGTCCAT CTAAAAATGG CTCCAAAGAC AAGGTCGAGA CAGATGATGC TCAGAGTGGT 
TCTGATCAGA AAAACAAAGC AGAAACAGAC GCTAAAGCAA AGCAGAAACA CACACCAGAC 
TGTGATGAGC CTTCACTTTC CTCTATGGAT CCAACAGTAT CTAGTGAAGG ACATCCAGAA 
AAATTACATT CAACCTCTGA GGTTCCAAAT CCTCCTGAAT CACCTCCTTG TGCAAGTAGC 
ATTGTTAAAG TACCTTCTGG AATTTTTGAC AGCACAGGAA GGAAAAGCAG TACCGGAGGT 
GGAACTGGAC CATTATCCCC GGTTTCAGAT GTTGGGGAAG ACCATGTGTT TTCCAGCGAA 
GGTCAAAACA AGAAGTCCAG TGAGAGAGCT CAAAAGAGGC AGAAAATGTT TGCTGAGCGA 
AGCTGTAGTT TCAGCGCAGA GAGCCGAGCT GGTATGCTAC TAAAGAAAGG CAGTCTTGAC 
CTAACCACTA ATGAAATTGC CATAAAGATG GGAGCGGATG CAAAAATACT TACTGCAGCT 
CTTTCAAAGA CACCACCACC CCAGCATGCT AAGAATGAAT TCTGCTTTGA AAGTATAGGT 
GAACAGTCTG TCTGTCAGAC ATCTGATAGC ACAGCTTGTG AACAAGATGA AAAACCTGAC 
ACAGAGACTG AGGAGCTAAC AGCCTCACGA CTGCAGGGGC CATCTGATCA GTTAGATACG 
GTGAAAGAGG AAAGCACAGT GGATGCTGGG GACTCTGTTA TAGGCACAGT GAAAGACAGC 
GTTGCTCTCA GGGATACTCC TAAACTTATA AGTGAACTTA ATAAAAGGCA TAGTTTTTAT 
GGCCTCACTA AATCCGTAGA AAGAGAAGGG GTAGAAAAGG GTCTTGACCC TCTATCTTTA 
ATGGCTTCAC AGACAGTGGA GAAAGAGCCA TCACCATCTG AGGAAAAGCA CATTTCCCCT 
GTTGTGGCAC GTAATCTGGC CGATGAGATT GAAAGTTACA TGAACCTGAA AAGTCCTCTT 
GGCTCCAAGT CTTCAAGTAT GGAGCTAGGA GGACAGGAGT GCAATGCAAG GGAATCTGGG 
AACCACAGCC AAGCTGGCCT GGAGCGGCGC TCTAGCTTAC CCGTAGACTC TTTTCCTCCA 
TCAGAAGTGC AGAGTGAAAA CCAGAAGAAC TCAGCTGTAT CCAGATCCAA GACTTTTACT 
AGCAGTCCAA AGCATCACCT ACGCAACCAA AAGGAACGAT CAGTTTCACT CACTGCCCTT 
GTCCGCAGCT CCCCACATGG CTCTTTGGGT TCGATGGTTA ACTCCTTTCC TGGGCTAAAA 
TTTGACAACC TTCTGACAGG ACCTAAAATG GATGTGCTGA AATCAGGCAT GAAGCAGGCA 
GCCACTGTAG CAAGTAAAGT TTGGGGGGCT GTTGCATCCG CTTATAGCTA CTCTGATGAT 
GAGGAGGAAA GTCAAAGTCA GAGAGGTCAG TCTAACTTTC CTGTAAGACC TCAAGAGTCT 
GGTGTTGGTG ACAATCTTTC CTTCAAGATG CCTTCAAATG GACTTTCTGG CAATGAGCTG 
ACACAGAGCA ATACCAGCTT AGGCAGCAGC AGCAGTGGAG ACACAAGCAA GCACAGCTAC 
TTAAATGAAT CTGTACAGGG GAAAGCATCA AAGGTTCTAG AGTCTGAAAA ATCAGAGCAA 
GGATCCTCTC ACAGCACCAG CTTGTCAAGC ATATTTCAGA ACTGGGCAAT GGAGGTCTTG 
ATGTCTAGTT GCTCTCAGTG CCGAGGTTGT GATGCACTTG TGTATGATGA AGAGATAATG 
GCTGGATGGA CTTTTGATGA CTCAAACTTA AATACTACTT GTCCCTTTTG TAAAAGACCC 
TTTTTACCAC TTCTTAATAT TGAGTTTAAA GACCTTCGTG GTACTGCAAG CTTTTTTCTT 
AAGCCAAGTA ACTCAGGAGA CAGTCTTCAT GGCAGTAACC TTCGGGTAAT GGCAGATTCG 
TCAGAGCGTA AACTGCAATC AGAATCTACA GCCAGTGACC TGATCAGCTT TGGGGAATCT 
TCCACTACTC CCCAAACCAC AGCAGAGGAA AGTAATACAG ACACTCAGCA GAGTGACCAG 
GCTGAAGAAG ATGGAACCAT GAAGTCTTCT GAGACAAACA ATGCACCAAA GCGAGGAGCG 
TCACTGACTA GGAGTCACAG CGTAGGAGGG CCTCTACAAA ACATTGACTT ATCCCAAAGG 
CCTTCTCATG GAATTTCCAC TGTCAGTCTT CCCAACAGCC TGCAAGAAGC TGTGGATCCT 
TTGGAAAAGC GGCACAACCC TCCTCCTGTC TCCGTCCCCT ACCTTAGTCC CCTTGTCCTA 
CGCAAGGAGC TGGAATCTTT GCTGGAGAAT GAGGGCGACC ATGTCATCTA CACATCCACT 
TTTATTAACC AGCATCCTAT TATCTTCTGG AACCTGGTTT GGTTCTTTAG ACGTTTGGAC 
CTGCCCAGCA ATTTGCCAGG GCTCATCCTC TCATCTGAGC ACTGCAATGG AGGGGTGCAG 
CTCCCGCTAT CATCTCTATC TCAGGACAGC AAGCTAGTCT ATATACAACT GTTGTGGGAC 
AACATAAACC TTCACCAGGA GCCCGGGGAG CCCCTTTACA GCAAATTTAG AGCCCTGGAC 
AATATCAAGA AGAAGGCTTC TCCTATCCCA TATGATCAAC AAAGCACCAA CAATTTACTG 
GAAAGCATTA CGCTTAGTAT CCAGCATAAT GACGTCCTTA GGCCTATCAG CCTGCTCCTG 
CAGAAATGCA GCACAACTGG AAAGAGGCAA AGGAGCTTGT ATAGAGAAAT ACTGTACCTT 
TCTTTGGTGT CTCTTGGAAG AGAAAATATC GACATAGAGG CCTTTGACAA TGAATATAGA 
ATCGCTTATG AAAAGCTCCC TCTTGAGATG CAGCAAAAAA TGCAGAAAAT TGATCAGCCT 
CCAAGCAAGA AGGTGGAATG CTGCAGGAGT ATCTTTGGAG CGCCCCTAAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935713.2
CDS	59..5932
Translation	MMEDKGPRVADYFVVAGLTDTFKLLDHDMGRIETKTLGPKAPITDIAVINKTAGETVPEGYTCIEITQSGLQANLNHGSLKSPEMFICYRREKGKPPLTDIGVLYEGKEHLLKGCEVIQATPYGRCANVNNSSGTSMRIFLTYRRSLPVRLQNSLAVTDICIIISNKGETPPHTFCKVDKNLNCGMWGSNVFLCYKKSVPASNSIPYKAGLIFRYPEEDNESFPLSESVPLFCLPMGATIECWDPQIRHPLPVFSTFVLTGSTAEKVYGAAIQFYETYPREMLSEKQMIQLGLITAVEKKVLTYKNINSKKCICLLSHWPFFETFRKFLMFIYKLSVSGPHTLPIEKHISHFMHNVPFPSPQRPRILVQLSAHDALVLSQPVATPMPLSGANFSTLLMNLGPENCASLLLFVLLECKILLHSLRPAVLTGVAEAVVAMIFPFQWQCPYIPLCPLSLADVLSAPIPFIVGVDSRYFDLYDPPQDVVCIDLDTSMVYTSEDKRNLSWKQLPKKPVKNLIGVLKKLYPRLSSVHQKSPEESAVLMAPIEADFSWQKKTTDLEMEIQEAFLRFMASILKGYRAYLKPITQAPSNKATAADSLFDRQGFLKSRDRAHIKFYTSLTKTQIFIRFIEECSFVSDKDTSLAFFDDCIDKLFPDKGVERGDKGDLEFPEDTRLIELDDSQKSEHTVFIMPPEPLPEDGPDPHPRYSYKNFPRLDYALFDRIRDQYLTFNGNTKSSRAASSPTFIAKRTKQEIKTAHKLARKYFSNDPAWGKCLFSQCFSLWFICLPVYVKVSHSKARALQKAYDVLVKMRKTDVDPLDEVCYRVVMQLCGIWENPALAVRVLFEMKNAGIQPNAITYGYYNKVVLVSPWPSSNRSGNFLWTKVRNVVFGLAQFRRATKKPQTSSESSSASALPSAAHANDGNAVSRQKVDNYNDSNSGEHTLFVRDLIRTDSTDNHSSTESPSKNGSKDKVETDDAQSGSDQKNKAETDAKAKQKHTPDCDEPSLSSMDPTVSSEGHPEKLHSTSEVPNPPESPPCASSIVKVPSGIFDSTGRKSSTGGGTGPLSPVSDVGEDHVFSSEGQNKKSSERAQKRQKMFAERSCSFSAESRAGMLLKKGSLDLTTNEIAIKMGADAKILTAALSKTPPPQHAKNEFCFESIGEQSVCQTSDSTACEQDEKPDTETEELTASRLQGPSDQLDTVKEESTVDAGDSVIGTVKDSVALRDTPKLISELNKRHSFYGLTKSVEREGVEKGLDPLSLMASQTVEKEPSPSEEKHISPVVARNLADEIESYMNLKSPLGSKSSSMELGGQECNARESGNHSQAGLERRSSLPVDSFPPSEVQSENQKNSAVSRSKTFTSSPKHHLRNQKERSVSLTALVRSSPHGSLGSMVNSFPGLKFDNLLTGPKMDVLKSGMKQAATVASKVWGAVASAYSYSDDEEESQSQRGQSNFPVRPQESGVGDNLSFKMPSNGLSGNELTQSNTSLGSSSSGDTSKHSYLNESVQGKASKVLESEKSEQGSSHSTSLSSIFQNWAMEVLMSSCSQCRGCDALVYDEEIMAGWTFDDSNLNTTCPFCKRPFLPLLNIEFKDLRGTASFFLKPSNSGDSLHGSNLRVMADSSERKLQSESTASDLISFGESSTTPQTTAEESNTDTQQSDQAEEDGTMKSSETNNAPKRGASLTRSHSVGGPLQNIDLSQRPSHGISTVSLPNSLQEAVDPLEKRHNPPPVSVPYLSPLVLRKELESLLENEGDHVIYTSTFINQHPIIFWNLVWFFRRLDLPSNLPGLILSSEHCNGGVQLPLSSLSQDSKLVYIQLLWDNINLHQEPGEPLYSKFRALDNIKKKASPIPYDQQSTNNLLESITLSIQHNDVLRPISLLLQKCSTTGKRQRSLYREILYLSLVSLGRENIDIEAFDNEYRIAYEKLPLEMQQKMQKIDQPPSKKVECCRSIFGAPLI

Target ORF information:

RefSeq Version	XM_002935667.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN domain containing 4C (dennd4c), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935667.5

ORF Insert Sequence:

ATGATGGAAG ACAAAGGGCC TCGGGTAGCA GACTACTTTG TTGTGGCAGG ACTGACAGAC 
ACCTTCAAGC TATTAGATCA TGACATGGGT CGGATAGAAA CTAAGACATT AGGTCCCAAA 
GCTCCAATCA CTGATATTGC TGTTATCAAC AAGACAGCTG GAGAGACCGT GCCAGAGGGC 
TACACATGTA TTGAAATCAC ACAAAGTGGG TTGCAGGCAA ATCTGAACCA TGGAAGCTTA 
AAGAGTCCAG AAATGTTTAT CTGTTACAGA CGAGAAAAAG GGAAACCTCC ACTAACAGAC 
ATTGGTGTGT TATATGAAGG AAAAGAACAC CTATTGAAAG GCTGTGAGGT TATCCAGGCT 
ACACCATATG GACGCTGCGC AAATGTCAAT AACAGCTCGG GTACCTCCAT GAGGATATTT 
CTAACTTACC GACGATCTTT ACCTGTTCGC CTTCAGAACT CCTTGGCAGT AACTGACATA 
TGTATAATCA TCAGTAATAA AGGAGAGACA CCACCCCATA CCTTCTGTAA GGTGGACAAA 
AACCTCAACT GTGGAATGTG GGGATCCAAT GTTTTCCTGT GTTATAAGAA GTCTGTCCCT 
GCATCTAACT CAATTCCTTA TAAAGCTGGT TTGATTTTCA GATATCCAGA GGAGGATAAC 
GAGTCATTTC CTCTATCAGA GTCCGTGCCA CTCTTTTGCC TTCCCATGGG AGCCACTATT 
GAATGCTGGG ACCCTCAAAT CCGACATCCT CTCCCTGTTT TCTCTACTTT TGTATTGACA 
GGTTCCACAG CTGAAAAGGT TTATGGTGCT GCTATTCAGT TCTATGAGAC TTACCCACGT 
GAAATGTTGT CTGAGAAGCA GATGATTCAG TTAGGACTGA TCACAGCTGT GGAGAAGAAA 
GTGCTCACCT ACAAAAATAT TAACTCCAAG AAGTGCATAT GTTTGCTTTC TCACTGGCCT 
TTCTTTGAGA CCTTCCGCAA GTTTCTTATG TTCATCTACA AGCTGTCTGT GTCTGGCCCT 
CATACCCTTC CTATTGAAAA GCACATATCG CACTTTATGC ACAATGTTCC CTTCCCATCA 
CCACAGAGGC CAAGAATTCT TGTTCAGCTC TCTGCTCATG ATGCCTTAGT TCTGTCCCAG 
CCAGTAGCAA CACCAATGCC TCTAAGTGGA GCAAATTTTA GTACACTGCT TATGAATCTT 
GGACCAGAAA ACTGTGCTTC TTTGCTGTTG TTCGTACTGC TAGAATGCAA GATTCTCCTT 
CACTCCCTCA GGCCAGCAGT GCTGACTGGA GTTGCTGAGG CTGTAGTAGC TATGATTTTC 
CCTTTCCAGT GGCAGTGCCC TTACATCCCC CTGTGCCCAC TGTCTCTCGC GGATGTGCTT 
AGTGCACCTA TTCCCTTTAT TGTGGGAGTG GATTCTCGAT ACTTTGATTT GTATGACCCG 
CCTCAGGATG TTGTTTGCAT CGACTTGGAC ACAAGCATGG TTTACACGTC TGAAGACAAG 
AGGAATTTAA GCTGGAAACA GCTTCCCAAG AAACCAGTGA AAAACCTTAT TGGCGTCTTA 
AAAAAGCTTT ACCCACGACT CTCTTCAGTT CACCAAAAGT CTCCAGAAGA GTCTGCTGTG 
CTGATGGCCC CAATTGAGGC AGACTTCTCA TGGCAAAAGA AAACAACAGA TTTGGAGATG 
GAAATTCAAG AGGCATTTCT ACGGTTCATG GCATCCATAC TCAAAGGTTA TAGGGCTTAC 
TTGAAACCCA TAACTCAAGC ACCATCCAAC AAAGCTACTG CTGCAGATTC ACTGTTTGAC 
CGTCAAGGGT TTTTGAAAAG TAGGGATCGT GCACACATAA AATTCTACAC TTCACTGACA 
AAAACCCAGA TTTTCATCCG CTTCATTGAA GAGTGCAGCT TTGTCAGTGA TAAGGACACC 
AGCCTGGCCT TCTTTGATGA CTGCATTGAT AAGTTGTTTC CTGATAAAGG AGTGGAGAGA 
GGTGATAAGG GGGATCTGGA GTTTCCAGAA GACACTCGTC TGATTGAGCT AGATGACTCA 
CAGAAGAGCG AGCACACTGT GTTTATAATG CCACCTGAGC CTCTGCCGGA AGATGGACCT 
GACCCTCACC CAAGATATAG CTACAAAAAC TTTCCAAGAC TAGACTACGC TCTTTTTGAC 
CGAATACGTG ATCAATATCT TACTTTCAAT GGGAATACTA AGAGTAGCAG AGCAGCAAGC 
AGCCCCACAT TTATTGCAAA AAGGACAAAA CAGGAAATCA AAACGGCTCA CAAGTTAGCT 
AGAAAATATT TTAGCAACGA TCCTGCTTGG GGCAAATGTC TCTTCAGCCA GTGCTTCAGT 
TTGTGGTTTA TTTGCCTGCC TGTTTATGTT AAGGTATCAC ATTCAAAAGC ACGTGCACTT 
CAGAAGGCTT ATGATGTGCT GGTTAAGATG AGGAAAACTG ATGTAGATCC ATTGGATGAG 
GTGTGTTACA GAGTGGTAAT GCAACTGTGT GGCATTTGGG AGAACCCTGC TCTGGCAGTG 
AGAGTATTAT TTGAGATGAA AAACGCTGGG ATACAGCCAA ATGCAATTAC CTATGGTTAC 
TATAATAAGG TTGTGTTGGT CAGCCCATGG CCAAGCAGTA ATCGCAGTGG AAACTTCCTA 
TGGACAAAAG TGCGCAATGT GGTGTTTGGT TTGGCTCAGT TCCGAAGGGC AACGAAGAAG 
CCACAGACAT CTTCCGAGTC CTCTTCGGCA TCAGCGCTTC CAAGTGCTGC ACATGCAAAT 
GATGGCAATG CTGTGAGCCG ACAAAAAGTG GATAATTATA ACGATTCTAA CTCTGGGGAG 
CACACCCTTT TCGTGCGCGA TCTAATCAGA ACTGATTCCA CTGATAACCA TTCTAGCACA 
GAGAGTCCAT CTAAAAATGG CTCCAAAGAC AAGGTCGAGA CAGATGATGC TCAGAGTGGT 
TCTGATCAGA AAAACAAAGC AGAAACAGAC GCTAAAGCAA AGCAGAAACA CACACCAGAC 
TGTGATGAGC CTTCACTTTC CTCTATGGAT CCAACAGTAT CTAGTGAAGG ACATCCAGAA 
AAATTACATT CAACCTCTGA GGTTCCAAAT CCTCCTGAAT CACCTCCTTG TGCAAGTAGC 
ATTGTTAAAG TACCTTCTGG AATTTTTGAC AGCACAGGAA GGAAAAGCAG TACCGGAGGT 
GGAACTGGAC CATTATCCCC GGTTTCAGAT GTTGGGGAAG ACCATGTGTT TTCCAGCGAA 
GGTCAAAACA AGAAGTCCAG TGAGAGAGCT CAAAAGAGGC AGAAAATGTT TGCTGAGCGA 
AGCTGTAGTT TCAGCGCAGA GAGCCGAGCT GGTATGCTAC TAAAGAAAGG CAGTCTTGAC 
CTAACCACTA ATGAAATTGC CATAAAGATG GGAGCGGATG CAAAAATACT TACTGCAGCT 
CTTTCAAAGA CACCACCACC CCAGCATGCT AAGAATGAAT TCTGCTTTGA AAGTATAGGT 
GAACAGTCTG TCTGTCAGAC ATCTGATAGC ACAGCTTGTG AACAAGATGA AAAACCTGAC 
ACAGAGACTG AGGAGCTAAC AGCCTCACGA CTGCAGGGGC CATCTGATCA GTTAGATACG 
GTGAAAGAGG AAAGCACAGT GGATGCTGGG GACTCTGTTA TAGGCACAGT GAAAGACAGC 
GTTGCTCTCA GGGATACTCC TAAACTTATA AGTGAACTTA ATAAAAGGCA TAGTTTTTAT 
GGCCTCACTA AATCCGTAGA AAGAGAAGGG GTAGAAAAGG GTCTTGACCC TCTATCTTTA 
ATGGCTTCAC AGACAGTGGA GAAAGAGCCA TCACCATCTG AGGAAAAGCA CATTTCCCCT 
GTTGTGGCAC GTAATCTGGC CGATGAGATT GAAAGTTACA TGAACCTGAA AAGTCCTCTT 
GGCTCCAAGT CTTCAAGTAT GGAGCTAGGA GGACAGGAGT GCAATGCAAG GGAATCTGGG 
AACCACAGCC AAGCTGGCCT GGAGCGGCGC TCTAGCTTAC CCGTAGACTC TTTTCCTCCA 
TCAGAAGTGC AGAGTGAAAA CCAGAAGAAC TCAGCTGTAT CCAGATCCAA GACTTTTACT 
AGCAGTCCAA AGCATCACCT ACGCAACCAA AAGGAACGAT CAGTTTCACT CACTGCCCTT 
GTCCGCAGCT CCCCACATGG CTCTTTGGGT TCGATGGTTA ACTCCTTTCC TGGGCTAAAA 
TTTGACAACC TTCTGACAGG ACCTAAAATG GATGTGCTGA AATCAGGCAT GAAGCAGGCA 
GCCACTGTAG CAAGTAAAGT TTGGGGGGCT GTTGCATCCG CTTATAGCTA CTCTGATGAT 
GAGGAGGAAA GTCAAAGTCA GAGAGGTCAG TCTAACTTTC CTGTAAGACC TCAAGAGTCT 
GGTGTTGGTG ACAATCTTTC CTTCAAGATG CCTTCAAATG GACTTTCTGG CAATGAGCTG 
ACACAGAGCA ATACCAGCTT AGGCAGCAGC AGCAGTGGAG ACACAAGCAA GCACAGCTAC 
TTAAATGAAT CTGTACAGGG GAAAGCATCA AAGGTTCTAG AGTCTGAAAA ATCAGAGCAA 
GGATCCTCTC ACAGCACCAG CTTGTCAAGC ATATTTCAGA ACTGGGCAAT GGAGGTCTTG 
ATGTCTAGTT GCTCTCAGTG CCGAGGTTGT GATGCACTTG TGTATGATGA AGAGATAATG 
GCTGGATGGA CTTTTGATGA CTCAAACTTA AATACTACTT GTCCCTTTTG TAAAAGACCC 
TTTTTACCAC TTCTTAATAT TGAGTTTAAA GACCTTCGTG GTACTGCAAG CTTTTTTCTT 
AAGCCAAGTA ACTCAGGAGA CAGTCTTCAT GGCAGTAACC TTCGGGTAAT GGCAGATTCG 
TCAGAGCGTA AACTGCAATC AGAATCTACA GCCAGTGACC TGATCAGCTT TGGGGAATCT 
TCCACTACTC CCCAAACCAC AGCAGAGGAA AGTAATACAG ACACTCAGCA GAGTGACCAG 
GCTGAAGAAG ATGGAACCAT GAAGTCTTCT GAGACAAACA ATGCACCAAA GCGAGGAGCG 
TCACTGACTA GGAGTCACAG CGTAGGAGGG CCTCTACAAA ACATTGACTT ATCCCAAAGG 
CCTTCTCATG GAATTTCCAC TGTCAGTCTT CCCAACAGCC TGCAAGAAGC TGTGGATCCT 
TTGGAAAAGC GGCACAACCC TCCTCCTGTC TCCGTCCCCT ACCTTAGTCC CCTTGTCCTA 
CGCAAGGAGC TGGAATCTTT GCTGGAGAAT GAGGGCGACC ATGTCATCTA CACATCCACT 
TTTATTAACC AGCATCCTAT TATCTTCTGG AACCTGGTTT GGTTCTTTAG ACGTTTGGAC 
CTGCCCAGCA ATTTGCCAGG GCTCATCCTC TCATCTGAGC ACTGCAATGG AGGGGTGCAG 
CTCCCGCTAT CATCTCTATC TCAGGACAGC AAGCTAGTCT ATATACAACT GTTGTGGGAC 
AACATAAACC TTCACCAGGA GCCCGGGGAG CCCCTTTACA GCAAATTTAG AGCCCTGGAC 
AATATCAAGA AGAAGGCTTC TCCTATCCCA TATGATCAAC AAAGCACCAA CAATTTACTG 
GAAAGCATTA CGCTTAGTAT CCAGCATAAT GACGTCCTTA GGCCTATCAG CCTGCTCCTG 
CAGAAATGCA GCACAACTGG AAAGAGGCAA AGGAGCTTGT ATAGAGAAAT ACTGTACCTT 
TCTTTGGTGT CTCTTGGAAG AGAAAATATC GACATAGAGG CCTTTGACAA TGAATATAGA 
ATCGCTTATG AAAAGCTCCC TCTTGAGATG CAGCAAAAAA TGCAGAAAAT TGATCAGCCT 
CCAAGCAAGA AGGTGGAATG CTGCAGGAGT ATCTTTGGAG CGCCCCTAAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd4c ( XM_018097779.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017953268.1 Xenopus tropicalis DENN/MADD domain containing 4C (dennd4c), transcript variant X4, mRNA.

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CloneID	OXa42090
Clone ID Related Accession (Same CDS sequence)	XM_018097779.1
Accession Version	XM_018097779.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DENN domain-containing protein 4C isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG ACAAAGGGCC TCGGGTAGCA GACTACTTTG TTGTGGCAGG ACTGACAGAC 
ACCTTCAAGC TATTAGATCA TGACATGGGT CGGATAGAAA CTAAGACATT AGGTCCCAAA 
GCTCCAATCA CTGATATTGC TGTTATCAAC AAGACAGCTG GAGAGACCGT GCCAGAGGGC 
TACACATGTA TTGAAATCAC ACAAAGTGGG TTGCAGGCAA ATCTGAACCA TGGAAGCTTA 
AAGAGTCCAG AAATGTTTAT CTGTTACAGA CGAGAAAAAG GGAAACCTCC ACTAACAGAC 
ATTGGTGTGT TATATGAAGG AAAAGAACAC CTATTGAAAG GCTGTGAGGT TATCCAGGCT 
ACACCATATG GACGCTGCGC AAATGTCAAT AACAGCTCGG GTACCTCCAT GAGGATATTT 
CTAACTTACC GACGATCTTT ACCTGTTCGC CTTCAGAACT CCTTGGCAGT AACTGACATA 
TGTATAATCA TCAGTAATAA AGGAGAGACA CCACCCCATA CCTTCTGTAA GGTGGACAAA 
AACCTCAACT GTGGAATGTG GGGATCCAAT GTTTTCCTGT GTTATAAGAA GTCTGTCCCT 
GCATCTAACT CAATTCCTTA TAAAGCTGGT TTGATTTTCA GATATCCAGA GGAGGATAAC 
GAGTCATTTC CTCTATCAGA GTCCGTGCCA CTCTTTTGCC TTCCCATGGG AGCCACTATT 
GAATGCTGGG ACCCTCAAAT CCGACATCCT CTCCCTGTTT TCTCTACTTT TGTATTGACA 
GGTTCCACAG CTGAAAAGGT TTATGGTGCT GCTATTCAGT TCTATGAGAC TTACCCACGT 
GAAATGTTGT CTGAGAAGCA GATGATTCAG TTAGGACTGA TCACAGCTGT GGAGAAGAAA 
GTGCTCACCT ACAAAAATAT TAACTCCAAG AAGTGCATAT GTTTGCTTTC TCACTGGCCT 
TTCTTTGAGA CCTTCCGCAA GTTTCTTATG TTCATCTACA AGCTGTCTGT GTCTGGCCCT 
CATACCCTTC CTATTGAAAA GCACATATCG CACTTTATGC ACAATGTTCC CTTCCCATCA 
CCACAGAGGC CAAGAATTCT TGTTCAGCTC TCTGCTCATG ATGCCTTAGT TCTGTCCCAG 
CCAGTAGCAA CACCAATGCC TCTAAGTGGA GCAAATTTTA GTACACTGCT TATGAATCTT 
GGACCAGAAA ACTGTGCTTC TTTGCTGTTG TTCGTACTGC TAGAATGCAA GATTCTCCTT 
CACTCCCTCA GGCCAGCAGT GCTGACTGGA GTTGCTGAGG CTGTAGTAGC TATGATTTTC 
CCTTTCCAGT GGCAGTGCCC TTACATCCCC CTGTGCCCAC TGTCTCTCGC GGATGTGCTT 
AGTGCACCTA TTCCCTTTAT TGTGGGAGTG GATTCTCGAT ACTTTGATTT GTATGACCCG 
CCTCAGGATG TTGTTTGCAT CGACTTGGAC ACAAGCATGG TTTACACGTC TGAAGACAAG 
AGGAATTTAA GCTGGAAACA GCTTCCCAAG AAACCAGTGA AAAACCTTAT TGGCGTCTTA 
AAAAAGCTTT ACCCACGACT CTCTTCAGTT CACCAAAAGT CTCCAGAAGA GTCTGCTGTG 
CTGATGGCCC CAATTGAGGC AGACTTCTCA TGGCAAAAGA AAACAACAGA TTTGGAGATG 
GAAATTCAAG AGGCATTTCT ACGGTTCATG GCATCCATAC TCAAAGGTTA TAGGGCTTAC 
TTGAAACCCA TAACTCAAGC ACCATCCAAC AAAGCTACTG CTGCAGATTC ACTGTTTGAC 
CGTCAAGGGT TTTTGAAAAG TAGGGATCGT GCACACATAA AATTCTACAC TTCACTGACA 
AAAACCCAGA TTTTCATCCG CTTCATTGAA GAGTGCAGCT TTGTCAGTGA TAAGGACACC 
AGCCTGGCCT TCTTTGATGA CTGCATTGAT AAGGGGGATC TGGAGTTTCC AGAAGACACT 
CGTCTGATTG AGCTAGATGA CTCACAGAAG AGCGAGCACA CTGTGTTTAT AATGCCACCT 
GAGCCTCTGC CGGAAGATGG ACCTGACCCT CACCCAAGAT ATAGCTACAA AAACTTTCCA 
AGACTAGACT ACGCTCTTTT TGACCGAATA CGTGATCAAT ATCTTACTTT CAATGGGAAT 
ACTAAGAGTA GCAGAGCAGC AAGCAGCCCC ACATTTATTG CAAAAAGGAC AAAACAGGAA 
ATCAAAACGG CTCACAAGTT AGCTAGAAAA TATTTTAGCA ACGATCCTGC TTGGGGCAAA 
TGTCTCTTCA GCCAGTGCTT CAGTTTGTGG TTTATTTGCC TGCCTGTTTA TGTTAAGGTA 
TCACATTCAA AAGCACGTGC ACTTCAGAAG GCTTATGATG TGCTGGTTAA GATGAGGAAA 
ACTGATGTAG ATCCATTGGA TGAGGTGTGT TACAGAGTGG TAATGCAACT GTGTGGCATT 
TGGGAGAACC CTGCTCTGGC AGTGAGAGTA TTATTTGAGA TGAAAAACGC TGGGATACAG 
CCAAATGCAA TTACCTATGG TTACTATAAT AAGGTTGTGT TGGTCAGCCC ATGGCCAAGC 
AGTAATCGCA GTGGAAACTT CCTATGGACA AAAGTGCGCA ATGTGGTGTT TGGTTTGGCT 
CAGTTCCGAA GGGCAACGAA GAAGCCACAG ACATCTTCCG AGTCCTCTTC GGCATCAGCG 
CTTCCAAGTG CTGCACATGC AAATGATGGC AATGCTGTGA GCCGACAAAA AGTGGATAAT 
TATAACGATT CTAACTCTGG GGAGCACACC CTTTTCGTGC GCGATCTAAT CAGAACTGAT 
TCCACTGATA ACCATTCTAG CACAGAGAGT CCATCTAAAA ATGGCTCCAA AGACAAGGTC 
GAGACAGATG ATGCTCAGAG TGGTTCTGAT CAGAAAAACA AAGCAGAAAC AGACGCTAAA 
GCAAAGCAGA AACACACACC AGACTGTGAT GAGCCTTCAC TTTCCTCTAT GGATCCAACA 
GTATCTAGTG AAGGACATCC AGAAAAATTA CATTCAACCT CTGAGGTTCC AAATCCTCCT 
GAATCACCTC CTTGTGCAAG TAGCATTGTT AAAGTACCTT CTGGAATTTT TGACAGCACA 
GGAAGGAAAA GCAGTACCGG AGGTGGAACT GGACCATTAT CCCCGGTTTC AGATGTTGGG 
GAAGACCATG TGTTTTCCAG CGAAGGTCAA AACAAGAAGT CCAGTGAGAG AGCTCAAAAG 
AGGCAGAAAA TGTTTGCTGA GCGAAGCTGT AGTTTCAGCG CAGAGAGCCG AGCTGGTATG 
CTACTAAAGA AAGGCAGTCT TGACCTAACC ACTAATGAAA TTGCCATAAA GATGGGAGCG 
GATGCAAAAA TACTTACTGC AGCTCTTTCA AAGACACCAC CACCCCAGCA TGCTAAGAAT 
GAATTCTGCT TTGAAAGTAT AGGTGAACAG TCTGTCTGTC AGACATCTGA TAGCACAGCT 
TGTGAACAAG ATGAAAAACC TGACACAGAG ACTGAGGAGC TAACAGCCTC ACGACTGCAG 
GGGCCATCTG ATCAGTTAGA TACGGTGAAA GAGGAAAGCA CAGTGGATGC TGGGGACTCT 
GTTATAGGCA CAGTGAAAGA CAGCGTTGCT CTCAGGGATA CTCCTAAACT TATAAGTGAA 
CTTAATAAAA GGCATAGTTT TTATGGCCTC ACTAAATCCG TAGAAAGAGA AGGGGTAGAA 
AAGGGTCTTG ACCCTCTATC TTTAATGGCT TCACAGACAG TGGAGAAAGA GCCATCACCA 
TCTGAGGAAA AGCACATTTC CCCTGTTGTG GCACGTAATC TGGCCGATGA GATTGAAAGT 
TACATGAACC TGAAAAGTCC TCTTGGCTCC AAGTCTTCAA GTATGGAGCT AGGAGGACAG 
GAGTGCAATG CAAGGGAATC TGGGAACCAC AGCCAAGCTG GCCTGGAGCG GCGCTCTAGC 
TTACCCGTAG ACTCTTTTCC TCCATCAGAA GTGCAGAGTG AAAACCAGAA GAACTCAGCT 
GTATCCAGAT CCAAGACTTT TACTAGCAGT CCAAAGCATC ACCTACGCAA CCAAAAGGAA 
CGATCAGTTT CACTCACTGC CCTTGTCCGC AGCTCCCCAC ATGGCTCTTT GGGTTCGATG 
GTTAACTCCT TTCCTGGGCT AAAATTTGAC AACCTTCTGA CAGGACCTAA AATGGATGTG 
CTGAAATCAG GCATGAAGCA GGCAGCCACT GTAGCAAGTA AAGTTTGGGG GGCTGTTGCA 
TCCGCTTATA GCTACTCTGA TGATGAGGAG GAAAGTCAAA GTCAGAGAGG TCAGTCTAAC 
TTTCCTGTAA GACCTCAAGA GTCTGGTGTT GGTGACAATC TTTCCTTCAA GATGCCTTCA 
AATGGACTTT CTGGCAATGA GCTGACACAG AGCAATACCA GCTTAGGCAG CAGCAGCAGT 
GGAGACACAA GCAAGCACAG CTACTTAAAT GAATCTGTAC AGGGGAAAGC ATCAAAGGTT 
CTAGAGTCTG AAAAATCAGA GCAAGGATCC TCTCACAGCA CCAGCTTGTC AAGCATATTT 
CAGAACTGGG CAATGGAGGT CTTGATGTCT AGTTGCTCTC AGTGCCGAGG TTGTGATGCA 
CTTGTGTATG ATGAAGAGAT AATGGCTGGA TGGACTTTTG ATGACTCAAA CTTAAATACT 
ACTTGTCCCT TTTGTAAAAG ACCCTTTTTA CCACTTCTTA ATATTGAGTT TAAAGACCTT 
CGTGGTACTG CAAGCTTTTT TCTTAAGCCA AGTAACTCAG GAGACAGTCT TCATGGCAGT 
AACCTTCGGG TAATGGCAGA TTCGTCAGAG CGTAAACTGC AATCAGAATC TACAGCCAGT 
GACCTGATCA GCTTTGGGGA ATCTTCCACT ACTCCCCAAA CCACAGCAGA GGAAAGTAAT 
ACAGACACTC AGCAGAGTGA CCAGGCTGAA GAAGATGGAA CCATGAAGTC TTCTGAGACA 
AACAATGCAC CAAAGCGAGG AGCGTCACTG ACTAGGAGTC ACAGCGTAGG AGGGCCTCTA 
CAAAACATTG ACTTATCCCA AAGGCCTTCT CATGGAATTT CCACTGTCAG TCTTCCCAAC 
AGCCTGCAAG AAGCTGTGGA TCCTTTGGAA AAGCGGCACA ACCCTCCTCC TGTCTCCGTC 
CCCTACCTTA GTCCCCTTGT CCTACGCAAG GAGCTGGAAT CTTTGCTGGA GAATGAGGGC 
GACCATGTCA TCTACACATC CACTTTTATT AACCAGCATC CTATTATCTT CTGGAACCTG 
GTTTGGTTCT TTAGACGTTT GGACCTGCCC AGCAATTTGC CAGGGCTCAT CCTCTCATCT 
GAGCACTGCA ATGGAGGGGT GCAGCTCCCG CTATCATCTC TATCTCAGGA CAGCAAGCTA 
GTCTATATAC AACTGTTGTG GGACAACATA AACCTTCACC AGGAGCCCGG GGAGCCCCTT 
TACAGCAAAT TTAGAGCCCT GGACAATATC AAGAAGAAGG CTTCTCCTAT CCCATATGAT 
CAACAAAGCA CCAACAATTT ACTGGAAAGC ATTACGCTTA GTATCCAGCA TAATGACGTC 
CTTAGGCCTA TCAGCCTGCT CCTGCAGAAA TGCAGCACAA CTGGAAAGAG GCAAAGGAGC 
TTGTATAGAG AAATACTGTA CCTTTCTTTG GTGTCTCTTG GAAGAGAAAA TATCGACATA 
GAGGCCTTTG ACAATGAATA TAGAATCGCT TATGAAAAGC TCCCTCTTGA GATGCAGCAA 
AAAATGCAGA AAATTGATCA GCCTCCAAGC AAGAAGGTGG AATGCTGCAG GAGTATCTTT 
GGAGCGCCCC TAATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017953268.1
CDS	226..6063
Translation	MMEDKGPRVADYFVVAGLTDTFKLLDHDMGRIETKTLGPKAPITDIAVINKTAGETVPEGYTCIEITQSGLQANLNHGSLKSPEMFICYRREKGKPPLTDIGVLYEGKEHLLKGCEVIQATPYGRCANVNNSSGTSMRIFLTYRRSLPVRLQNSLAVTDICIIISNKGETPPHTFCKVDKNLNCGMWGSNVFLCYKKSVPASNSIPYKAGLIFRYPEEDNESFPLSESVPLFCLPMGATIECWDPQIRHPLPVFSTFVLTGSTAEKVYGAAIQFYETYPREMLSEKQMIQLGLITAVEKKVLTYKNINSKKCICLLSHWPFFETFRKFLMFIYKLSVSGPHTLPIEKHISHFMHNVPFPSPQRPRILVQLSAHDALVLSQPVATPMPLSGANFSTLLMNLGPENCASLLLFVLLECKILLHSLRPAVLTGVAEAVVAMIFPFQWQCPYIPLCPLSLADVLSAPIPFIVGVDSRYFDLYDPPQDVVCIDLDTSMVYTSEDKRNLSWKQLPKKPVKNLIGVLKKLYPRLSSVHQKSPEESAVLMAPIEADFSWQKKTTDLEMEIQEAFLRFMASILKGYRAYLKPITQAPSNKATAADSLFDRQGFLKSRDRAHIKFYTSLTKTQIFIRFIEECSFVSDKDTSLAFFDDCIDKGDLEFPEDTRLIELDDSQKSEHTVFIMPPEPLPEDGPDPHPRYSYKNFPRLDYALFDRIRDQYLTFNGNTKSSRAASSPTFIAKRTKQEIKTAHKLARKYFSNDPAWGKCLFSQCFSLWFICLPVYVKVSHSKARALQKAYDVLVKMRKTDVDPLDEVCYRVVMQLCGIWENPALAVRVLFEMKNAGIQPNAITYGYYNKVVLVSPWPSSNRSGNFLWTKVRNVVFGLAQFRRATKKPQTSSESSSASALPSAAHANDGNAVSRQKVDNYNDSNSGEHTLFVRDLIRTDSTDNHSSTESPSKNGSKDKVETDDAQSGSDQKNKAETDAKAKQKHTPDCDEPSLSSMDPTVSSEGHPEKLHSTSEVPNPPESPPCASSIVKVPSGIFDSTGRKSSTGGGTGPLSPVSDVGEDHVFSSEGQNKKSSERAQKRQKMFAERSCSFSAESRAGMLLKKGSLDLTTNEIAIKMGADAKILTAALSKTPPPQHAKNEFCFESIGEQSVCQTSDSTACEQDEKPDTETEELTASRLQGPSDQLDTVKEESTVDAGDSVIGTVKDSVALRDTPKLISELNKRHSFYGLTKSVEREGVEKGLDPLSLMASQTVEKEPSPSEEKHISPVVARNLADEIESYMNLKSPLGSKSSSMELGGQECNARESGNHSQAGLERRSSLPVDSFPPSEVQSENQKNSAVSRSKTFTSSPKHHLRNQKERSVSLTALVRSSPHGSLGSMVNSFPGLKFDNLLTGPKMDVLKSGMKQAATVASKVWGAVASAYSYSDDEEESQSQRGQSNFPVRPQESGVGDNLSFKMPSNGLSGNELTQSNTSLGSSSSGDTSKHSYLNESVQGKASKVLESEKSEQGSSHSTSLSSIFQNWAMEVLMSSCSQCRGCDALVYDEEIMAGWTFDDSNLNTTCPFCKRPFLPLLNIEFKDLRGTASFFLKPSNSGDSLHGSNLRVMADSSERKLQSESTASDLISFGESSTTPQTTAEESNTDTQQSDQAEEDGTMKSSETNNAPKRGASLTRSHSVGGPLQNIDLSQRPSHGISTVSLPNSLQEAVDPLEKRHNPPPVSVPYLSPLVLRKELESLLENEGDHVIYTSTFINQHPIIFWNLVWFFRRLDLPSNLPGLILSSEHCNGGVQLPLSSLSQDSKLVYIQLLWDNINLHQEPGEPLYSKFRALDNIKKKASPIPYDQQSTNNLLESITLSIQHNDVLRPISLLLQKCSTTGKRQRSLYREILYLSLVSLGRENIDIEAFDNEYRIAYEKLPLEMQQKMQKIDQPPSKKVECCRSIFGAPLI

Target ORF information:

RefSeq Version	XM_018097779.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN/MADD domain containing 4C (dennd4c), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018097779.1

ORF Insert Sequence:

ATGATGGAAG ACAAAGGGCC TCGGGTAGCA GACTACTTTG TTGTGGCAGG ACTGACAGAC 
ACCTTCAAGC TATTAGATCA TGACATGGGT CGGATAGAAA CTAAGACATT AGGTCCCAAA 
GCTCCAATCA CTGATATTGC TGTTATCAAC AAGACAGCTG GAGAGACCGT GCCAGAGGGC 
TACACATGTA TTGAAATCAC ACAAAGTGGG TTGCAGGCAA ATCTGAACCA TGGAAGCTTA 
AAGAGTCCAG AAATGTTTAT CTGTTACAGA CGAGAAAAAG GGAAACCTCC ACTAACAGAC 
ATTGGTGTGT TATATGAAGG AAAAGAACAC CTATTGAAAG GCTGTGAGGT TATCCAGGCT 
ACACCATATG GACGCTGCGC AAATGTCAAT AACAGCTCGG GTACCTCCAT GAGGATATTT 
CTAACTTACC GACGATCTTT ACCTGTTCGC CTTCAGAACT CCTTGGCAGT AACTGACATA 
TGTATAATCA TCAGTAATAA AGGAGAGACA CCACCCCATA CCTTCTGTAA GGTGGACAAA 
AACCTCAACT GTGGAATGTG GGGATCCAAT GTTTTCCTGT GTTATAAGAA GTCTGTCCCT 
GCATCTAACT CAATTCCTTA TAAAGCTGGT TTGATTTTCA GATATCCAGA GGAGGATAAC 
GAGTCATTTC CTCTATCAGA GTCCGTGCCA CTCTTTTGCC TTCCCATGGG AGCCACTATT 
GAATGCTGGG ACCCTCAAAT CCGACATCCT CTCCCTGTTT TCTCTACTTT TGTATTGACA 
GGTTCCACAG CTGAAAAGGT TTATGGTGCT GCTATTCAGT TCTATGAGAC TTACCCACGT 
GAAATGTTGT CTGAGAAGCA GATGATTCAG TTAGGACTGA TCACAGCTGT GGAGAAGAAA 
GTGCTCACCT ACAAAAATAT TAACTCCAAG AAGTGCATAT GTTTGCTTTC TCACTGGCCT 
TTCTTTGAGA CCTTCCGCAA GTTTCTTATG TTCATCTACA AGCTGTCTGT GTCTGGCCCT 
CATACCCTTC CTATTGAAAA GCACATATCG CACTTTATGC ACAATGTTCC CTTCCCATCA 
CCACAGAGGC CAAGAATTCT TGTTCAGCTC TCTGCTCATG ATGCCTTAGT TCTGTCCCAG 
CCAGTAGCAA CACCAATGCC TCTAAGTGGA GCAAATTTTA GTACACTGCT TATGAATCTT 
GGACCAGAAA ACTGTGCTTC TTTGCTGTTG TTCGTACTGC TAGAATGCAA GATTCTCCTT 
CACTCCCTCA GGCCAGCAGT GCTGACTGGA GTTGCTGAGG CTGTAGTAGC TATGATTTTC 
CCTTTCCAGT GGCAGTGCCC TTACATCCCC CTGTGCCCAC TGTCTCTCGC GGATGTGCTT 
AGTGCACCTA TTCCCTTTAT TGTGGGAGTG GATTCTCGAT ACTTTGATTT GTATGACCCG 
CCTCAGGATG TTGTTTGCAT CGACTTGGAC ACAAGCATGG TTTACACGTC TGAAGACAAG 
AGGAATTTAA GCTGGAAACA GCTTCCCAAG AAACCAGTGA AAAACCTTAT TGGCGTCTTA 
AAAAAGCTTT ACCCACGACT CTCTTCAGTT CACCAAAAGT CTCCAGAAGA GTCTGCTGTG 
CTGATGGCCC CAATTGAGGC AGACTTCTCA TGGCAAAAGA AAACAACAGA TTTGGAGATG 
GAAATTCAAG AGGCATTTCT ACGGTTCATG GCATCCATAC TCAAAGGTTA TAGGGCTTAC 
TTGAAACCCA TAACTCAAGC ACCATCCAAC AAAGCTACTG CTGCAGATTC ACTGTTTGAC 
CGTCAAGGGT TTTTGAAAAG TAGGGATCGT GCACACATAA AATTCTACAC TTCACTGACA 
AAAACCCAGA TTTTCATCCG CTTCATTGAA GAGTGCAGCT TTGTCAGTGA TAAGGACACC 
AGCCTGGCCT TCTTTGATGA CTGCATTGAT AAGGGGGATC TGGAGTTTCC AGAAGACACT 
CGTCTGATTG AGCTAGATGA CTCACAGAAG AGCGAGCACA CTGTGTTTAT AATGCCACCT 
GAGCCTCTGC CGGAAGATGG ACCTGACCCT CACCCAAGAT ATAGCTACAA AAACTTTCCA 
AGACTAGACT ACGCTCTTTT TGACCGAATA CGTGATCAAT ATCTTACTTT CAATGGGAAT 
ACTAAGAGTA GCAGAGCAGC AAGCAGCCCC ACATTTATTG CAAAAAGGAC AAAACAGGAA 
ATCAAAACGG CTCACAAGTT AGCTAGAAAA TATTTTAGCA ACGATCCTGC TTGGGGCAAA 
TGTCTCTTCA GCCAGTGCTT CAGTTTGTGG TTTATTTGCC TGCCTGTTTA TGTTAAGGTA 
TCACATTCAA AAGCACGTGC ACTTCAGAAG GCTTATGATG TGCTGGTTAA GATGAGGAAA 
ACTGATGTAG ATCCATTGGA TGAGGTGTGT TACAGAGTGG TAATGCAACT GTGTGGCATT 
TGGGAGAACC CTGCTCTGGC AGTGAGAGTA TTATTTGAGA TGAAAAACGC TGGGATACAG 
CCAAATGCAA TTACCTATGG TTACTATAAT AAGGTTGTGT TGGTCAGCCC ATGGCCAAGC 
AGTAATCGCA GTGGAAACTT CCTATGGACA AAAGTGCGCA ATGTGGTGTT TGGTTTGGCT 
CAGTTCCGAA GGGCAACGAA GAAGCCACAG ACATCTTCCG AGTCCTCTTC GGCATCAGCG 
CTTCCAAGTG CTGCACATGC AAATGATGGC AATGCTGTGA GCCGACAAAA AGTGGATAAT 
TATAACGATT CTAACTCTGG GGAGCACACC CTTTTCGTGC GCGATCTAAT CAGAACTGAT 
TCCACTGATA ACCATTCTAG CACAGAGAGT CCATCTAAAA ATGGCTCCAA AGACAAGGTC 
GAGACAGATG ATGCTCAGAG TGGTTCTGAT CAGAAAAACA AAGCAGAAAC AGACGCTAAA 
GCAAAGCAGA AACACACACC AGACTGTGAT GAGCCTTCAC TTTCCTCTAT GGATCCAACA 
GTATCTAGTG AAGGACATCC AGAAAAATTA CATTCAACCT CTGAGGTTCC AAATCCTCCT 
GAATCACCTC CTTGTGCAAG TAGCATTGTT AAAGTACCTT CTGGAATTTT TGACAGCACA 
GGAAGGAAAA GCAGTACCGG AGGTGGAACT GGACCATTAT CCCCGGTTTC AGATGTTGGG 
GAAGACCATG TGTTTTCCAG CGAAGGTCAA AACAAGAAGT CCAGTGAGAG AGCTCAAAAG 
AGGCAGAAAA TGTTTGCTGA GCGAAGCTGT AGTTTCAGCG CAGAGAGCCG AGCTGGTATG 
CTACTAAAGA AAGGCAGTCT TGACCTAACC ACTAATGAAA TTGCCATAAA GATGGGAGCG 
GATGCAAAAA TACTTACTGC AGCTCTTTCA AAGACACCAC CACCCCAGCA TGCTAAGAAT 
GAATTCTGCT TTGAAAGTAT AGGTGAACAG TCTGTCTGTC AGACATCTGA TAGCACAGCT 
TGTGAACAAG ATGAAAAACC TGACACAGAG ACTGAGGAGC TAACAGCCTC ACGACTGCAG 
GGGCCATCTG ATCAGTTAGA TACGGTGAAA GAGGAAAGCA CAGTGGATGC TGGGGACTCT 
GTTATAGGCA CAGTGAAAGA CAGCGTTGCT CTCAGGGATA CTCCTAAACT TATAAGTGAA 
CTTAATAAAA GGCATAGTTT TTATGGCCTC ACTAAATCCG TAGAAAGAGA AGGGGTAGAA 
AAGGGTCTTG ACCCTCTATC TTTAATGGCT TCACAGACAG TGGAGAAAGA GCCATCACCA 
TCTGAGGAAA AGCACATTTC CCCTGTTGTG GCACGTAATC TGGCCGATGA GATTGAAAGT 
TACATGAACC TGAAAAGTCC TCTTGGCTCC AAGTCTTCAA GTATGGAGCT AGGAGGACAG 
GAGTGCAATG CAAGGGAATC TGGGAACCAC AGCCAAGCTG GCCTGGAGCG GCGCTCTAGC 
TTACCCGTAG ACTCTTTTCC TCCATCAGAA GTGCAGAGTG AAAACCAGAA GAACTCAGCT 
GTATCCAGAT CCAAGACTTT TACTAGCAGT CCAAAGCATC ACCTACGCAA CCAAAAGGAA 
CGATCAGTTT CACTCACTGC CCTTGTCCGC AGCTCCCCAC ATGGCTCTTT GGGTTCGATG 
GTTAACTCCT TTCCTGGGCT AAAATTTGAC AACCTTCTGA CAGGACCTAA AATGGATGTG 
CTGAAATCAG GCATGAAGCA GGCAGCCACT GTAGCAAGTA AAGTTTGGGG GGCTGTTGCA 
TCCGCTTATA GCTACTCTGA TGATGAGGAG GAAAGTCAAA GTCAGAGAGG TCAGTCTAAC 
TTTCCTGTAA GACCTCAAGA GTCTGGTGTT GGTGACAATC TTTCCTTCAA GATGCCTTCA 
AATGGACTTT CTGGCAATGA GCTGACACAG AGCAATACCA GCTTAGGCAG CAGCAGCAGT 
GGAGACACAA GCAAGCACAG CTACTTAAAT GAATCTGTAC AGGGGAAAGC ATCAAAGGTT 
CTAGAGTCTG AAAAATCAGA GCAAGGATCC TCTCACAGCA CCAGCTTGTC AAGCATATTT 
CAGAACTGGG CAATGGAGGT CTTGATGTCT AGTTGCTCTC AGTGCCGAGG TTGTGATGCA 
CTTGTGTATG ATGAAGAGAT AATGGCTGGA TGGACTTTTG ATGACTCAAA CTTAAATACT 
ACTTGTCCCT TTTGTAAAAG ACCCTTTTTA CCACTTCTTA ATATTGAGTT TAAAGACCTT 
CGTGGTACTG CAAGCTTTTT TCTTAAGCCA AGTAACTCAG GAGACAGTCT TCATGGCAGT 
AACCTTCGGG TAATGGCAGA TTCGTCAGAG CGTAAACTGC AATCAGAATC TACAGCCAGT 
GACCTGATCA GCTTTGGGGA ATCTTCCACT ACTCCCCAAA CCACAGCAGA GGAAAGTAAT 
ACAGACACTC AGCAGAGTGA CCAGGCTGAA GAAGATGGAA CCATGAAGTC TTCTGAGACA 
AACAATGCAC CAAAGCGAGG AGCGTCACTG ACTAGGAGTC ACAGCGTAGG AGGGCCTCTA 
CAAAACATTG ACTTATCCCA AAGGCCTTCT CATGGAATTT CCACTGTCAG TCTTCCCAAC 
AGCCTGCAAG AAGCTGTGGA TCCTTTGGAA AAGCGGCACA ACCCTCCTCC TGTCTCCGTC 
CCCTACCTTA GTCCCCTTGT CCTACGCAAG GAGCTGGAAT CTTTGCTGGA GAATGAGGGC 
GACCATGTCA TCTACACATC CACTTTTATT AACCAGCATC CTATTATCTT CTGGAACCTG 
GTTTGGTTCT TTAGACGTTT GGACCTGCCC AGCAATTTGC CAGGGCTCAT CCTCTCATCT 
GAGCACTGCA ATGGAGGGGT GCAGCTCCCG CTATCATCTC TATCTCAGGA CAGCAAGCTA 
GTCTATATAC AACTGTTGTG GGACAACATA AACCTTCACC AGGAGCCCGG GGAGCCCCTT 
TACAGCAAAT TTAGAGCCCT GGACAATATC AAGAAGAAGG CTTCTCCTAT CCCATATGAT 
CAACAAAGCA CCAACAATTT ACTGGAAAGC ATTACGCTTA GTATCCAGCA TAATGACGTC 
CTTAGGCCTA TCAGCCTGCT CCTGCAGAAA TGCAGCACAA CTGGAAAGAG GCAAAGGAGC 
TTGTATAGAG AAATACTGTA CCTTTCTTTG GTGTCTCTTG GAAGAGAAAA TATCGACATA 
GAGGCCTTTG ACAATGAATA TAGAATCGCT TATGAAAAGC TCCCTCTTGA GATGCAGCAA 
AAAATGCAGA AAATTGATCA GCCTCCAAGC AAGAAGGTGG AATGCTGCAG GAGTATCTTT 
GGAGCGCCCC TAATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd4c ( XM_004910863.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910920.1 Xenopus tropicalis DENN/MADD domain containing 4C (dennd4c), transcript variant X2, mRNA.

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CloneID	OXa04068
Clone ID Related Accession (Same CDS sequence)	XM_004910862.4 , XM_004910863.3 , XM_004910863.4 , XM_004910862.3 , XM_002935667.4 , XM_002935667.5
Accession Version	XM_004910863.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DENN domain-containing protein 4C isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910863.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG ACAAAGGGCC TCGGGTAGCA GACTACTTTG TTGTGGCAGG ACTGACAGAC 
ACCTTCAAGC TATTAGATCA TGACATGGGT CGGATAGAAA CTAAGACATT AGGTCCCAAA 
GCTCCAATCA CTGATATTGC TGTTATCAAC AAGACAGCTG GAGAGACCGT GCCAGAGGGC 
TACACATGTA TTGAAATCAC ACAAAGTGGG TTGCAGGCAA ATCTGAACCA TGGAAGCTTA 
AAGAGTCCAG AAATGTTTAT CTGTTACAGA CGAGAAAAAG GGAAACCTCC ACTAACAGAC 
ATTGGTGTGT TATATGAAGG AAAAGAACAC CTATTGAAAG GCTGTGAGGT TATCCAGGCT 
ACACCATATG GACGCTGCGC AAATGTCAAT AACAGCTCGG GTACCTCCAT GAGGATATTT 
CTAACTTACC GACGATCTTT ACCTGTTCGC CTTCAGAACT CCTTGGCAGT AACTGACATA 
TGTATAATCA TCAGTAATAA AGGAGAGACA CCACCCCATA CCTTCTGTAA GGTGGACAAA 
AACCTCAACT GTGGAATGTG GGGATCCAAT GTTTTCCTGT GTTATAAGAA GTCTGTCCCT 
GCATCTAACT CAATTCCTTA TAAAGCTGGT TTGATTTTCA GATATCCAGA GGAGGATAAC 
GAGTCATTTC CTCTATCAGA GTCCGTGCCA CTCTTTTGCC TTCCCATGGG AGCCACTATT 
GAATGCTGGG ACCCTCAAAT CCGACATCCT CTCCCTGTTT TCTCTACTTT TGTATTGACA 
GGTTCCACAG CTGAAAAGGT TTATGGTGCT GCTATTCAGT TCTATGAGAC TTACCCACGT 
GAAATGTTGT CTGAGAAGCA GATGATTCAG TTAGGACTGA TCACAGCTGT GGAGAAGAAA 
GTGCTCACCT ACAAAAATAT TAACTCCAAG AAGTGCATAT GTTTGCTTTC TCACTGGCCT 
TTCTTTGAGA CCTTCCGCAA GTTTCTTATG TTCATCTACA AGCTGTCTGT GTCTGGCCCT 
CATACCCTTC CTATTGAAAA GCACATATCG CACTTTATGC ACAATGTTCC CTTCCCATCA 
CCACAGAGGC CAAGAATTCT TGTTCAGCTC TCTGCTCATG ATGCCTTAGT TCTGTCCCAG 
CCAGTAGCAA CACCAATGCC TCTAAGTGGA GCAAATTTTA GTACACTGCT TATGAATCTT 
GGACCAGAAA ACTGTGCTTC TTTGCTGTTG TTCGTACTGC TAGAATGCAA GATTCTCCTT 
CACTCCCTCA GGCCAGCAGT GCTGACTGGA GTTGCTGAGG CTGTAGTAGC TATGATTTTC 
CCTTTCCAGT GGCAGTGCCC TTACATCCCC CTGTGCCCAC TGTCTCTCGC GGATGTGCTT 
AGTGCACCTA TTCCCTTTAT TGTGGGAGTG GATTCTCGAT ACTTTGATTT GTATGACCCG 
CCTCAGGATG TTGTTTGCAT CGACTTGGAC ACAAGCATGG TTTACACGTC TGAAGACAAG 
AGGAATTTAA GCTGGAAACA GCTTCCCAAG AAACCAGTGA AAAACCTTAT TGGCGTCTTA 
AAAAAGCTTT ACCCACGACT CTCTTCAGTT CACCAAAAGT CTCCAGAAGA GTCTGCTGTG 
CTGATGGCCC CAATTGAGGC AGACTTCTCA TGGCAAAAGA AAACAACAGA TTTGGAGATG 
GAAATTCAAG AGGCATTTCT ACGGTTCATG GCATCCATAC TCAAAGGTTA TAGGGCTTAC 
TTGAAACCCA TAACTCAAGC ACCATCCAAC AAAGCTACTG CTGCAGATTC ACTGTTTGAC 
CGTCAAGGGT TTTTGAAAAG TAGGGATCGT GCACACATAA AATTCTACAC TTCACTGACA 
AAAACCCAGA TTTTCATCCG CTTCATTGAA GAGTGCAGCT TTGTCAGTGA TAAGGACACC 
AGCCTGGCCT TCTTTGATGA CTGCATTGAT AAGTTGTTTC CTGATAAAGG AGTGGAGAGA 
GGTGATAAGG GGGATCTGGA GTTTCCAGAA GACACTCGTC TGATTGAGCT AGATGACTCA 
CAGAAGAGCG AGCACACTGT GTTTATAATG CCACCTGAGC CTCTGCCGGA AGATGGACCT 
GACCCTCACC CAAGATATAG CTACAAAAAC TTTCCAAGAC TAGACTACGC TCTTTTTGAC 
CGAATACGTG ATCAATATCT TACTTTCAAT GGGAATACTA AGAGTAGCAG AGCAGCAAGC 
AGCCCCACAT TTATTGCAAA AAGGACAAAA CAGGAAATCA AAACGGCTCA CAAGTTAGCT 
AGAAAATATT TTAGCAACGA TCCTGCTTGG GGCAAATGTC TCTTCAGCCA GTGCTTCAGT 
TTGTGGTTTA TTTGCCTGCC TGTTTATGTT AAGGTATCAC ATTCAAAAGC ACGTGCACTT 
CAGAAGGCTT ATGATGTGCT GGTTAAGATG AGGAAAACTG ATGTAGATCC ATTGGATGAG 
GTGTGTTACA GAGTGGTAAT GCAACTGTGT GGCATTTGGG AGAACCCTGC TCTGGCAGTG 
AGAGTATTAT TTGAGATGAA AAACGCTGGG ATACAGCCAA ATGCAATTAC CTATGGTTAC 
TATAATAAGG TTGTGTTGGT CAGCCCATGG CCAAGCAGTA ATCGCAGTGG AAACTTCCTA 
TGGACAAAAG TGCGCAATGT GGTGTTTGGT TTGGCTCAGT TCCGAAGGGC AACGAAGAAG 
CCACAGACAT CTTCCGAGTC CTCTTCGGCA TCAGCGCTTC CAAGTGCTGC ACATGCAAAT 
GATGGCAATG CTGTGAGCCG ACAAAAAGTG GATAATTATA ACGATTCTAA CTCTGGGGAG 
CACACCCTTT TCGTGCGCGA TCTAATCAGA ACTGATTCCA CTGATAACCA TTCTAGCACA 
GAGAGTCCAT CTAAAAATGG CTCCAAAGAC AAGGTCGAGA CAGATGATGC TCAGAGTGGT 
TCTGATCAGA AAAACAAAGC AGAAACAGAC GCTAAAGCAA AGCAGAAACA CACACCAGAC 
TGTGATGAGC CTTCACTTTC CTCTATGGAT CCAACAGTAT CTAGTGAAGG ACATCCAGAA 
AAATTACATT CAACCTCTGA GGTTCCAAAT CCTCCTGAAT CACCTCCTTG TGCAAGTAGC 
ATTGTTAAAG TACCTTCTGG AATTTTTGAC AGCACAGGAA GGAAAAGCAG TACCGGAGGT 
GGAACTGGAC CATTATCCCC GGTTTCAGAT GTTGGGGAAG ACCATGTGTT TTCCAGCGAA 
GGTCAAAACA AGAAGTCCAG TGAGAGAGCT CAAAAGAGGC AGAAAATGTT TGCTGAGCGA 
AGCTGTAGTT TCAGCGCAGA GAGCCGAGCT GGTATGCTAC TAAAGAAAGG CAGTCTTGAC 
CTAACCACTA ATGAAATTGC CATAAAGATG GGAGCGGATG CAAAAATACT TACTGCAGCT 
CTTTCAAAGA CACCACCACC CCAGCATGCT AAGAATGAAT TCTGCTTTGA AAGTATAGGT 
GAACAGTCTG TCTGTCAGAC ATCTGATAGC ACAGCTTGTG AACAAGATGA AAAACCTGAC 
ACAGAGACTG AGGAGCTAAC AGCCTCACGA CTGCAGGGGC CATCTGATCA GTTAGATACG 
GTGAAAGAGG AAAGCACAGT GGATGCTGGG GACTCTGTTA TAGGCACAGT GAAAGACAGC 
GTTGCTCTCA GGGATACTCC TAAACTTATA AGTGAACTTA ATAAAAGGCA TAGTTTTTAT 
GGCCTCACTA AATCCGTAGA AAGAGAAGGG GTAGAAAAGG GTCTTGACCC TCTATCTTTA 
ATGGCTTCAC AGACAGTGGA GAAAGAGCCA TCACCATCTG AGGAAAAGCA CATTTCCCCT 
GTTGTGGCAC GTAATCTGGC CGATGAGATT GAAAGTTACA TGAACCTGAA AAGTCCTCTT 
GGCTCCAAGT CTTCAAGTAT GGAGCTAGGA GGACAGGAGT GCAATGCAAG GGAATCTGGG 
AACCACAGCC AAGCTGGCCT GGAGCGGCGC TCTAGCTTAC CCGTAGACTC TTTTCCTCCA 
TCAGAAGTGC AGAGTGAAAA CCAGAAGAAC TCAGCTGTAT CCAGATCCAA GACTTTTACT 
AGCAGTCCAA AGCATCACCT ACGCAACCAA AAGGAACGAT CAGTTTCACT CACTGCCCTT 
GTCCGCAGCT CCCCACATGG CTCTTTGGGT TCGATGGTTA ACTCCTTTCC TGGGCTAAAA 
TTTGACAACC TTCTGACAGG ACCTAAAATG GATGTGCTGA AATCAGGCAT GAAGCAGGCA 
GCCACTGTAG CAAGTAAAGT TTGGGGGGCT GTTGCATCCG CTTATAGCTA CTCTGATGAT 
GAGGAGGAAA GTCAAAGTCA GAGAGGTCAG TCTAACTTTC CTGTAAGACC TCAAGAGTCT 
GGTGTTGGTG ACAATCTTTC CTTCAAGATG CCTTCAAATG GACTTTCTGG CAATGAGCTG 
ACACAGAGCA ATACCAGCTT AGGCAGCAGC AGCAGTGGAG ACACAAGCAA GCACAGCTAC 
TTAAATGAAT CTGTACAGGG GAAAGCATCA AAGGTTCTAG AGTCTGAAAA ATCAGAGCAA 
GGATCCTCTC ACAGCACCAG CTTGTCAAGC ATATTTCAGA ACTGGGCAAT GGAGGTCTTG 
ATGTCTAGTT GCTCTCAGTG CCGAGGTTGT GATGCACTTG TGTATGATGA AGAGATAATG 
GCTGGATGGA CTTTTGATGA CTCAAACTTA AATACTACTT GTCCCTTTTG TAAAAGACCC 
TTTTTACCAC TTCTTAATAT TGAGTTTAAA GACCTTCGTG GTACTGCAAG CTTTTTTCTT 
AAGCCAAGTA ACTCAGGAGA CAGTCTTCAT GGCAGTAACC TTCGGGTAAT GGCAGATTCG 
TCAGAGCGTA AACTGCAATC AGAATCTACA GCCAGTGACC TGATCAGCTT TGGGGAATCT 
TCCACTACTC CCCAAACCAC AGCAGAGGAA AGTAATACAG ACACTCAGCA GAGTGACCAG 
GCTGAAGAAG ATGGAACCAT GAAGTCTTCT GAGACAAACA ATGCACCAAA GCGAGGAGCG 
TCACTGACTA GGAGTCACAG CGTAGGAGGG CCTCTACAAA ACATTGACTT ATCCCAAAGG 
CCTTCTCATG GAATTTCCAC TGTCAGTCTT CCCAACAGCC TGCAAGAAGC TGTGGATCCT 
TTGGAAAAGC GGCACAACCC TCCTCCTGTC TCCGTCCCCT ACCTTAGTCC CCTTGTCCTA 
CGCAAGGAGC TGGAATCTTT GCTGGAGAAT GAGGGCGACC ATGTCATCTA CACATCCACT 
TTTATTAACC AGCATCCTAT TATCTTCTGG AACCTGGTTT GGTTCTTTAG ACGTTTGGAC 
CTGCCCAGCA ATTTGCCAGG GCTCATCCTC TCATCTGAGC ACTGCAATGG AGGGGTGCAG 
CTCCCGCTAT CATCTCTATC TCAGGACAGC AAGCTAGTCT ATATACAACT GTTGTGGGAC 
AACATAAACC TTCACCAGGA GCCCGGGGAG CCCCTTTACA GCAAATTTAG AGCCCTGGAC 
AATATCAAGA AGAAGGCTTC TCCTATCCCA TATGATCAAC AAAGCACCAA CAATTTACTG 
GAAAGCATTA CGCTTAGTAT CCAGCATAAT GACGTCCTTA GGCCTATCAG CCTGCTCCTG 
CAGAAATGCA GCACAACTGG AAAGAGGCAA AGGAGCTTGT ATAGAGAAAT ACTGTACCTT 
TCTTTGGTGT CTCTTGGAAG AGAAAATATC GACATAGAGG CCTTTGACAA TGAATATAGA 
ATCGCTTATG AAAAGCTCCC TCTTGAGATG CAGCAAAAAA TGCAGAAAAT TGATCAGCCT 
CCAAGCAAGA AGGTGGAATG CTGCAGGAGT ATCTTTGGAG CGCCCCTAAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910920.1
CDS	102..5975
Translation	MMEDKGPRVADYFVVAGLTDTFKLLDHDMGRIETKTLGPKAPITDIAVINKTAGETVPEGYTCIEITQSGLQANLNHGSLKSPEMFICYRREKGKPPLTDIGVLYEGKEHLLKGCEVIQATPYGRCANVNNSSGTSMRIFLTYRRSLPVRLQNSLAVTDICIIISNKGETPPHTFCKVDKNLNCGMWGSNVFLCYKKSVPASNSIPYKAGLIFRYPEEDNESFPLSESVPLFCLPMGATIECWDPQIRHPLPVFSTFVLTGSTAEKVYGAAIQFYETYPREMLSEKQMIQLGLITAVEKKVLTYKNINSKKCICLLSHWPFFETFRKFLMFIYKLSVSGPHTLPIEKHISHFMHNVPFPSPQRPRILVQLSAHDALVLSQPVATPMPLSGANFSTLLMNLGPENCASLLLFVLLECKILLHSLRPAVLTGVAEAVVAMIFPFQWQCPYIPLCPLSLADVLSAPIPFIVGVDSRYFDLYDPPQDVVCIDLDTSMVYTSEDKRNLSWKQLPKKPVKNLIGVLKKLYPRLSSVHQKSPEESAVLMAPIEADFSWQKKTTDLEMEIQEAFLRFMASILKGYRAYLKPITQAPSNKATAADSLFDRQGFLKSRDRAHIKFYTSLTKTQIFIRFIEECSFVSDKDTSLAFFDDCIDKLFPDKGVERGDKGDLEFPEDTRLIELDDSQKSEHTVFIMPPEPLPEDGPDPHPRYSYKNFPRLDYALFDRIRDQYLTFNGNTKSSRAASSPTFIAKRTKQEIKTAHKLARKYFSNDPAWGKCLFSQCFSLWFICLPVYVKVSHSKARALQKAYDVLVKMRKTDVDPLDEVCYRVVMQLCGIWENPALAVRVLFEMKNAGIQPNAITYGYYNKVVLVSPWPSSNRSGNFLWTKVRNVVFGLAQFRRATKKPQTSSESSSASALPSAAHANDGNAVSRQKVDNYNDSNSGEHTLFVRDLIRTDSTDNHSSTESPSKNGSKDKVETDDAQSGSDQKNKAETDAKAKQKHTPDCDEPSLSSMDPTVSSEGHPEKLHSTSEVPNPPESPPCASSIVKVPSGIFDSTGRKSSTGGGTGPLSPVSDVGEDHVFSSEGQNKKSSERAQKRQKMFAERSCSFSAESRAGMLLKKGSLDLTTNEIAIKMGADAKILTAALSKTPPPQHAKNEFCFESIGEQSVCQTSDSTACEQDEKPDTETEELTASRLQGPSDQLDTVKEESTVDAGDSVIGTVKDSVALRDTPKLISELNKRHSFYGLTKSVEREGVEKGLDPLSLMASQTVEKEPSPSEEKHISPVVARNLADEIESYMNLKSPLGSKSSSMELGGQECNARESGNHSQAGLERRSSLPVDSFPPSEVQSENQKNSAVSRSKTFTSSPKHHLRNQKERSVSLTALVRSSPHGSLGSMVNSFPGLKFDNLLTGPKMDVLKSGMKQAATVASKVWGAVASAYSYSDDEEESQSQRGQSNFPVRPQESGVGDNLSFKMPSNGLSGNELTQSNTSLGSSSSGDTSKHSYLNESVQGKASKVLESEKSEQGSSHSTSLSSIFQNWAMEVLMSSCSQCRGCDALVYDEEIMAGWTFDDSNLNTTCPFCKRPFLPLLNIEFKDLRGTASFFLKPSNSGDSLHGSNLRVMADSSERKLQSESTASDLISFGESSTTPQTTAEESNTDTQQSDQAEEDGTMKSSETNNAPKRGASLTRSHSVGGPLQNIDLSQRPSHGISTVSLPNSLQEAVDPLEKRHNPPPVSVPYLSPLVLRKELESLLENEGDHVIYTSTFINQHPIIFWNLVWFFRRLDLPSNLPGLILSSEHCNGGVQLPLSSLSQDSKLVYIQLLWDNINLHQEPGEPLYSKFRALDNIKKKASPIPYDQQSTNNLLESITLSIQHNDVLRPISLLLQKCSTTGKRQRSLYREILYLSLVSLGRENIDIEAFDNEYRIAYEKLPLEMQQKMQKIDQPPSKKVECCRSIFGAPLI

Target ORF information:

RefSeq Version	XM_004910863.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN/MADD domain containing 4C (dennd4c), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910863.3

ORF Insert Sequence:

ATGATGGAAG ACAAAGGGCC TCGGGTAGCA GACTACTTTG TTGTGGCAGG ACTGACAGAC 
ACCTTCAAGC TATTAGATCA TGACATGGGT CGGATAGAAA CTAAGACATT AGGTCCCAAA 
GCTCCAATCA CTGATATTGC TGTTATCAAC AAGACAGCTG GAGAGACCGT GCCAGAGGGC 
TACACATGTA TTGAAATCAC ACAAAGTGGG TTGCAGGCAA ATCTGAACCA TGGAAGCTTA 
AAGAGTCCAG AAATGTTTAT CTGTTACAGA CGAGAAAAAG GGAAACCTCC ACTAACAGAC 
ATTGGTGTGT TATATGAAGG AAAAGAACAC CTATTGAAAG GCTGTGAGGT TATCCAGGCT 
ACACCATATG GACGCTGCGC AAATGTCAAT AACAGCTCGG GTACCTCCAT GAGGATATTT 
CTAACTTACC GACGATCTTT ACCTGTTCGC CTTCAGAACT CCTTGGCAGT AACTGACATA 
TGTATAATCA TCAGTAATAA AGGAGAGACA CCACCCCATA CCTTCTGTAA GGTGGACAAA 
AACCTCAACT GTGGAATGTG GGGATCCAAT GTTTTCCTGT GTTATAAGAA GTCTGTCCCT 
GCATCTAACT CAATTCCTTA TAAAGCTGGT TTGATTTTCA GATATCCAGA GGAGGATAAC 
GAGTCATTTC CTCTATCAGA GTCCGTGCCA CTCTTTTGCC TTCCCATGGG AGCCACTATT 
GAATGCTGGG ACCCTCAAAT CCGACATCCT CTCCCTGTTT TCTCTACTTT TGTATTGACA 
GGTTCCACAG CTGAAAAGGT TTATGGTGCT GCTATTCAGT TCTATGAGAC TTACCCACGT 
GAAATGTTGT CTGAGAAGCA GATGATTCAG TTAGGACTGA TCACAGCTGT GGAGAAGAAA 
GTGCTCACCT ACAAAAATAT TAACTCCAAG AAGTGCATAT GTTTGCTTTC TCACTGGCCT 
TTCTTTGAGA CCTTCCGCAA GTTTCTTATG TTCATCTACA AGCTGTCTGT GTCTGGCCCT 
CATACCCTTC CTATTGAAAA GCACATATCG CACTTTATGC ACAATGTTCC CTTCCCATCA 
CCACAGAGGC CAAGAATTCT TGTTCAGCTC TCTGCTCATG ATGCCTTAGT TCTGTCCCAG 
CCAGTAGCAA CACCAATGCC TCTAAGTGGA GCAAATTTTA GTACACTGCT TATGAATCTT 
GGACCAGAAA ACTGTGCTTC TTTGCTGTTG TTCGTACTGC TAGAATGCAA GATTCTCCTT 
CACTCCCTCA GGCCAGCAGT GCTGACTGGA GTTGCTGAGG CTGTAGTAGC TATGATTTTC 
CCTTTCCAGT GGCAGTGCCC TTACATCCCC CTGTGCCCAC TGTCTCTCGC GGATGTGCTT 
AGTGCACCTA TTCCCTTTAT TGTGGGAGTG GATTCTCGAT ACTTTGATTT GTATGACCCG 
CCTCAGGATG TTGTTTGCAT CGACTTGGAC ACAAGCATGG TTTACACGTC TGAAGACAAG 
AGGAATTTAA GCTGGAAACA GCTTCCCAAG AAACCAGTGA AAAACCTTAT TGGCGTCTTA 
AAAAAGCTTT ACCCACGACT CTCTTCAGTT CACCAAAAGT CTCCAGAAGA GTCTGCTGTG 
CTGATGGCCC CAATTGAGGC AGACTTCTCA TGGCAAAAGA AAACAACAGA TTTGGAGATG 
GAAATTCAAG AGGCATTTCT ACGGTTCATG GCATCCATAC TCAAAGGTTA TAGGGCTTAC 
TTGAAACCCA TAACTCAAGC ACCATCCAAC AAAGCTACTG CTGCAGATTC ACTGTTTGAC 
CGTCAAGGGT TTTTGAAAAG TAGGGATCGT GCACACATAA AATTCTACAC TTCACTGACA 
AAAACCCAGA TTTTCATCCG CTTCATTGAA GAGTGCAGCT TTGTCAGTGA TAAGGACACC 
AGCCTGGCCT TCTTTGATGA CTGCATTGAT AAGTTGTTTC CTGATAAAGG AGTGGAGAGA 
GGTGATAAGG GGGATCTGGA GTTTCCAGAA GACACTCGTC TGATTGAGCT AGATGACTCA 
CAGAAGAGCG AGCACACTGT GTTTATAATG CCACCTGAGC CTCTGCCGGA AGATGGACCT 
GACCCTCACC CAAGATATAG CTACAAAAAC TTTCCAAGAC TAGACTACGC TCTTTTTGAC 
CGAATACGTG ATCAATATCT TACTTTCAAT GGGAATACTA AGAGTAGCAG AGCAGCAAGC 
AGCCCCACAT TTATTGCAAA AAGGACAAAA CAGGAAATCA AAACGGCTCA CAAGTTAGCT 
AGAAAATATT TTAGCAACGA TCCTGCTTGG GGCAAATGTC TCTTCAGCCA GTGCTTCAGT 
TTGTGGTTTA TTTGCCTGCC TGTTTATGTT AAGGTATCAC ATTCAAAAGC ACGTGCACTT 
CAGAAGGCTT ATGATGTGCT GGTTAAGATG AGGAAAACTG ATGTAGATCC ATTGGATGAG 
GTGTGTTACA GAGTGGTAAT GCAACTGTGT GGCATTTGGG AGAACCCTGC TCTGGCAGTG 
AGAGTATTAT TTGAGATGAA AAACGCTGGG ATACAGCCAA ATGCAATTAC CTATGGTTAC 
TATAATAAGG TTGTGTTGGT CAGCCCATGG CCAAGCAGTA ATCGCAGTGG AAACTTCCTA 
TGGACAAAAG TGCGCAATGT GGTGTTTGGT TTGGCTCAGT TCCGAAGGGC AACGAAGAAG 
CCACAGACAT CTTCCGAGTC CTCTTCGGCA TCAGCGCTTC CAAGTGCTGC ACATGCAAAT 
GATGGCAATG CTGTGAGCCG ACAAAAAGTG GATAATTATA ACGATTCTAA CTCTGGGGAG 
CACACCCTTT TCGTGCGCGA TCTAATCAGA ACTGATTCCA CTGATAACCA TTCTAGCACA 
GAGAGTCCAT CTAAAAATGG CTCCAAAGAC AAGGTCGAGA CAGATGATGC TCAGAGTGGT 
TCTGATCAGA AAAACAAAGC AGAAACAGAC GCTAAAGCAA AGCAGAAACA CACACCAGAC 
TGTGATGAGC CTTCACTTTC CTCTATGGAT CCAACAGTAT CTAGTGAAGG ACATCCAGAA 
AAATTACATT CAACCTCTGA GGTTCCAAAT CCTCCTGAAT CACCTCCTTG TGCAAGTAGC 
ATTGTTAAAG TACCTTCTGG AATTTTTGAC AGCACAGGAA GGAAAAGCAG TACCGGAGGT 
GGAACTGGAC CATTATCCCC GGTTTCAGAT GTTGGGGAAG ACCATGTGTT TTCCAGCGAA 
GGTCAAAACA AGAAGTCCAG TGAGAGAGCT CAAAAGAGGC AGAAAATGTT TGCTGAGCGA 
AGCTGTAGTT TCAGCGCAGA GAGCCGAGCT GGTATGCTAC TAAAGAAAGG CAGTCTTGAC 
CTAACCACTA ATGAAATTGC CATAAAGATG GGAGCGGATG CAAAAATACT TACTGCAGCT 
CTTTCAAAGA CACCACCACC CCAGCATGCT AAGAATGAAT TCTGCTTTGA AAGTATAGGT 
GAACAGTCTG TCTGTCAGAC ATCTGATAGC ACAGCTTGTG AACAAGATGA AAAACCTGAC 
ACAGAGACTG AGGAGCTAAC AGCCTCACGA CTGCAGGGGC CATCTGATCA GTTAGATACG 
GTGAAAGAGG AAAGCACAGT GGATGCTGGG GACTCTGTTA TAGGCACAGT GAAAGACAGC 
GTTGCTCTCA GGGATACTCC TAAACTTATA AGTGAACTTA ATAAAAGGCA TAGTTTTTAT 
GGCCTCACTA AATCCGTAGA AAGAGAAGGG GTAGAAAAGG GTCTTGACCC TCTATCTTTA 
ATGGCTTCAC AGACAGTGGA GAAAGAGCCA TCACCATCTG AGGAAAAGCA CATTTCCCCT 
GTTGTGGCAC GTAATCTGGC CGATGAGATT GAAAGTTACA TGAACCTGAA AAGTCCTCTT 
GGCTCCAAGT CTTCAAGTAT GGAGCTAGGA GGACAGGAGT GCAATGCAAG GGAATCTGGG 
AACCACAGCC AAGCTGGCCT GGAGCGGCGC TCTAGCTTAC CCGTAGACTC TTTTCCTCCA 
TCAGAAGTGC AGAGTGAAAA CCAGAAGAAC TCAGCTGTAT CCAGATCCAA GACTTTTACT 
AGCAGTCCAA AGCATCACCT ACGCAACCAA AAGGAACGAT CAGTTTCACT CACTGCCCTT 
GTCCGCAGCT CCCCACATGG CTCTTTGGGT TCGATGGTTA ACTCCTTTCC TGGGCTAAAA 
TTTGACAACC TTCTGACAGG ACCTAAAATG GATGTGCTGA AATCAGGCAT GAAGCAGGCA 
GCCACTGTAG CAAGTAAAGT TTGGGGGGCT GTTGCATCCG CTTATAGCTA CTCTGATGAT 
GAGGAGGAAA GTCAAAGTCA GAGAGGTCAG TCTAACTTTC CTGTAAGACC TCAAGAGTCT 
GGTGTTGGTG ACAATCTTTC CTTCAAGATG CCTTCAAATG GACTTTCTGG CAATGAGCTG 
ACACAGAGCA ATACCAGCTT AGGCAGCAGC AGCAGTGGAG ACACAAGCAA GCACAGCTAC 
TTAAATGAAT CTGTACAGGG GAAAGCATCA AAGGTTCTAG AGTCTGAAAA ATCAGAGCAA 
GGATCCTCTC ACAGCACCAG CTTGTCAAGC ATATTTCAGA ACTGGGCAAT GGAGGTCTTG 
ATGTCTAGTT GCTCTCAGTG CCGAGGTTGT GATGCACTTG TGTATGATGA AGAGATAATG 
GCTGGATGGA CTTTTGATGA CTCAAACTTA AATACTACTT GTCCCTTTTG TAAAAGACCC 
TTTTTACCAC TTCTTAATAT TGAGTTTAAA GACCTTCGTG GTACTGCAAG CTTTTTTCTT 
AAGCCAAGTA ACTCAGGAGA CAGTCTTCAT GGCAGTAACC TTCGGGTAAT GGCAGATTCG 
TCAGAGCGTA AACTGCAATC AGAATCTACA GCCAGTGACC TGATCAGCTT TGGGGAATCT 
TCCACTACTC CCCAAACCAC AGCAGAGGAA AGTAATACAG ACACTCAGCA GAGTGACCAG 
GCTGAAGAAG ATGGAACCAT GAAGTCTTCT GAGACAAACA ATGCACCAAA GCGAGGAGCG 
TCACTGACTA GGAGTCACAG CGTAGGAGGG CCTCTACAAA ACATTGACTT ATCCCAAAGG 
CCTTCTCATG GAATTTCCAC TGTCAGTCTT CCCAACAGCC TGCAAGAAGC TGTGGATCCT 
TTGGAAAAGC GGCACAACCC TCCTCCTGTC TCCGTCCCCT ACCTTAGTCC CCTTGTCCTA 
CGCAAGGAGC TGGAATCTTT GCTGGAGAAT GAGGGCGACC ATGTCATCTA CACATCCACT 
TTTATTAACC AGCATCCTAT TATCTTCTGG AACCTGGTTT GGTTCTTTAG ACGTTTGGAC 
CTGCCCAGCA ATTTGCCAGG GCTCATCCTC TCATCTGAGC ACTGCAATGG AGGGGTGCAG 
CTCCCGCTAT CATCTCTATC TCAGGACAGC AAGCTAGTCT ATATACAACT GTTGTGGGAC 
AACATAAACC TTCACCAGGA GCCCGGGGAG CCCCTTTACA GCAAATTTAG AGCCCTGGAC 
AATATCAAGA AGAAGGCTTC TCCTATCCCA TATGATCAAC AAAGCACCAA CAATTTACTG 
GAAAGCATTA CGCTTAGTAT CCAGCATAAT GACGTCCTTA GGCCTATCAG CCTGCTCCTG 
CAGAAATGCA GCACAACTGG AAAGAGGCAA AGGAGCTTGT ATAGAGAAAT ACTGTACCTT 
TCTTTGGTGT CTCTTGGAAG AGAAAATATC GACATAGAGG CCTTTGACAA TGAATATAGA 
ATCGCTTATG AAAAGCTCCC TCTTGAGATG CAGCAAAAAA TGCAGAAAAT TGATCAGCCT 
CCAAGCAAGA AGGTGGAATG CTGCAGGAGT ATCTTTGGAG CGCCCCTAAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd4c ( XM_004910862.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910919.1 Xenopus tropicalis DENN/MADD domain containing 4C (dennd4c), transcript variant X1, mRNA.

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CloneID	OXa04068
Clone ID Related Accession (Same CDS sequence)	XM_004910862.4 , XM_004910863.3 , XM_004910863.4 , XM_004910862.3 , XM_002935667.4 , XM_002935667.5
Accession Version	XM_004910862.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DENN domain-containing protein 4C isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910862.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG ACAAAGGGCC TCGGGTAGCA GACTACTTTG TTGTGGCAGG ACTGACAGAC 
ACCTTCAAGC TATTAGATCA TGACATGGGT CGGATAGAAA CTAAGACATT AGGTCCCAAA 
GCTCCAATCA CTGATATTGC TGTTATCAAC AAGACAGCTG GAGAGACCGT GCCAGAGGGC 
TACACATGTA TTGAAATCAC ACAAAGTGGG TTGCAGGCAA ATCTGAACCA TGGAAGCTTA 
AAGAGTCCAG AAATGTTTAT CTGTTACAGA CGAGAAAAAG GGAAACCTCC ACTAACAGAC 
ATTGGTGTGT TATATGAAGG AAAAGAACAC CTATTGAAAG GCTGTGAGGT TATCCAGGCT 
ACACCATATG GACGCTGCGC AAATGTCAAT AACAGCTCGG GTACCTCCAT GAGGATATTT 
CTAACTTACC GACGATCTTT ACCTGTTCGC CTTCAGAACT CCTTGGCAGT AACTGACATA 
TGTATAATCA TCAGTAATAA AGGAGAGACA CCACCCCATA CCTTCTGTAA GGTGGACAAA 
AACCTCAACT GTGGAATGTG GGGATCCAAT GTTTTCCTGT GTTATAAGAA GTCTGTCCCT 
GCATCTAACT CAATTCCTTA TAAAGCTGGT TTGATTTTCA GATATCCAGA GGAGGATAAC 
GAGTCATTTC CTCTATCAGA GTCCGTGCCA CTCTTTTGCC TTCCCATGGG AGCCACTATT 
GAATGCTGGG ACCCTCAAAT CCGACATCCT CTCCCTGTTT TCTCTACTTT TGTATTGACA 
GGTTCCACAG CTGAAAAGGT TTATGGTGCT GCTATTCAGT TCTATGAGAC TTACCCACGT 
GAAATGTTGT CTGAGAAGCA GATGATTCAG TTAGGACTGA TCACAGCTGT GGAGAAGAAA 
GTGCTCACCT ACAAAAATAT TAACTCCAAG AAGTGCATAT GTTTGCTTTC TCACTGGCCT 
TTCTTTGAGA CCTTCCGCAA GTTTCTTATG TTCATCTACA AGCTGTCTGT GTCTGGCCCT 
CATACCCTTC CTATTGAAAA GCACATATCG CACTTTATGC ACAATGTTCC CTTCCCATCA 
CCACAGAGGC CAAGAATTCT TGTTCAGCTC TCTGCTCATG ATGCCTTAGT TCTGTCCCAG 
CCAGTAGCAA CACCAATGCC TCTAAGTGGA GCAAATTTTA GTACACTGCT TATGAATCTT 
GGACCAGAAA ACTGTGCTTC TTTGCTGTTG TTCGTACTGC TAGAATGCAA GATTCTCCTT 
CACTCCCTCA GGCCAGCAGT GCTGACTGGA GTTGCTGAGG CTGTAGTAGC TATGATTTTC 
CCTTTCCAGT GGCAGTGCCC TTACATCCCC CTGTGCCCAC TGTCTCTCGC GGATGTGCTT 
AGTGCACCTA TTCCCTTTAT TGTGGGAGTG GATTCTCGAT ACTTTGATTT GTATGACCCG 
CCTCAGGATG TTGTTTGCAT CGACTTGGAC ACAAGCATGG TTTACACGTC TGAAGACAAG 
AGGAATTTAA GCTGGAAACA GCTTCCCAAG AAACCAGTGA AAAACCTTAT TGGCGTCTTA 
AAAAAGCTTT ACCCACGACT CTCTTCAGTT CACCAAAAGT CTCCAGAAGA GTCTGCTGTG 
CTGATGGCCC CAATTGAGGC AGACTTCTCA TGGCAAAAGA AAACAACAGA TTTGGAGATG 
GAAATTCAAG AGGCATTTCT ACGGTTCATG GCATCCATAC TCAAAGGTTA TAGGGCTTAC 
TTGAAACCCA TAACTCAAGC ACCATCCAAC AAAGCTACTG CTGCAGATTC ACTGTTTGAC 
CGTCAAGGGT TTTTGAAAAG TAGGGATCGT GCACACATAA AATTCTACAC TTCACTGACA 
AAAACCCAGA TTTTCATCCG CTTCATTGAA GAGTGCAGCT TTGTCAGTGA TAAGGACACC 
AGCCTGGCCT TCTTTGATGA CTGCATTGAT AAGTTGTTTC CTGATAAAGG AGTGGAGAGA 
GGTGATAAGG GGGATCTGGA GTTTCCAGAA GACACTCGTC TGATTGAGCT AGATGACTCA 
CAGAAGAGCG AGCACACTGT GTTTATAATG CCACCTGAGC CTCTGCCGGA AGATGGACCT 
GACCCTCACC CAAGATATAG CTACAAAAAC TTTCCAAGAC TAGACTACGC TCTTTTTGAC 
CGAATACGTG ATCAATATCT TACTTTCAAT GGGAATACTA AGAGTAGCAG AGCAGCAAGC 
AGCCCCACAT TTATTGCAAA AAGGACAAAA CAGGAAATCA AAACGGCTCA CAAGTTAGCT 
AGAAAATATT TTAGCAACGA TCCTGCTTGG GGCAAATGTC TCTTCAGCCA GTGCTTCAGT 
TTGTGGTTTA TTTGCCTGCC TGTTTATGTT AAGGTATCAC ATTCAAAAGC ACGTGCACTT 
CAGAAGGCTT ATGATGTGCT GGTTAAGATG AGGAAAACTG ATGTAGATCC ATTGGATGAG 
GTGTGTTACA GAGTGGTAAT GCAACTGTGT GGCATTTGGG AGAACCCTGC TCTGGCAGTG 
AGAGTATTAT TTGAGATGAA AAACGCTGGG ATACAGCCAA ATGCAATTAC CTATGGTTAC 
TATAATAAGG TTGTGTTGGT CAGCCCATGG CCAAGCAGTA ATCGCAGTGG AAACTTCCTA 
TGGACAAAAG TGCGCAATGT GGTGTTTGGT TTGGCTCAGT TCCGAAGGGC AACGAAGAAG 
CCACAGACAT CTTCCGAGTC CTCTTCGGCA TCAGCGCTTC CAAGTGCTGC ACATGCAAAT 
GATGGCAATG CTGTGAGCCG ACAAAAAGTG GATAATTATA ACGATTCTAA CTCTGGGGAG 
CACACCCTTT TCGTGCGCGA TCTAATCAGA ACTGATTCCA CTGATAACCA TTCTAGCACA 
GAGAGTCCAT CTAAAAATGG CTCCAAAGAC AAGGTCGAGA CAGATGATGC TCAGAGTGGT 
TCTGATCAGA AAAACAAAGC AGAAACAGAC GCTAAAGCAA AGCAGAAACA CACACCAGAC 
TGTGATGAGC CTTCACTTTC CTCTATGGAT CCAACAGTAT CTAGTGAAGG ACATCCAGAA 
AAATTACATT CAACCTCTGA GGTTCCAAAT CCTCCTGAAT CACCTCCTTG TGCAAGTAGC 
ATTGTTAAAG TACCTTCTGG AATTTTTGAC AGCACAGGAA GGAAAAGCAG TACCGGAGGT 
GGAACTGGAC CATTATCCCC GGTTTCAGAT GTTGGGGAAG ACCATGTGTT TTCCAGCGAA 
GGTCAAAACA AGAAGTCCAG TGAGAGAGCT CAAAAGAGGC AGAAAATGTT TGCTGAGCGA 
AGCTGTAGTT TCAGCGCAGA GAGCCGAGCT GGTATGCTAC TAAAGAAAGG CAGTCTTGAC 
CTAACCACTA ATGAAATTGC CATAAAGATG GGAGCGGATG CAAAAATACT TACTGCAGCT 
CTTTCAAAGA CACCACCACC CCAGCATGCT AAGAATGAAT TCTGCTTTGA AAGTATAGGT 
GAACAGTCTG TCTGTCAGAC ATCTGATAGC ACAGCTTGTG AACAAGATGA AAAACCTGAC 
ACAGAGACTG AGGAGCTAAC AGCCTCACGA CTGCAGGGGC CATCTGATCA GTTAGATACG 
GTGAAAGAGG AAAGCACAGT GGATGCTGGG GACTCTGTTA TAGGCACAGT GAAAGACAGC 
GTTGCTCTCA GGGATACTCC TAAACTTATA AGTGAACTTA ATAAAAGGCA TAGTTTTTAT 
GGCCTCACTA AATCCGTAGA AAGAGAAGGG GTAGAAAAGG GTCTTGACCC TCTATCTTTA 
ATGGCTTCAC AGACAGTGGA GAAAGAGCCA TCACCATCTG AGGAAAAGCA CATTTCCCCT 
GTTGTGGCAC GTAATCTGGC CGATGAGATT GAAAGTTACA TGAACCTGAA AAGTCCTCTT 
GGCTCCAAGT CTTCAAGTAT GGAGCTAGGA GGACAGGAGT GCAATGCAAG GGAATCTGGG 
AACCACAGCC AAGCTGGCCT GGAGCGGCGC TCTAGCTTAC CCGTAGACTC TTTTCCTCCA 
TCAGAAGTGC AGAGTGAAAA CCAGAAGAAC TCAGCTGTAT CCAGATCCAA GACTTTTACT 
AGCAGTCCAA AGCATCACCT ACGCAACCAA AAGGAACGAT CAGTTTCACT CACTGCCCTT 
GTCCGCAGCT CCCCACATGG CTCTTTGGGT TCGATGGTTA ACTCCTTTCC TGGGCTAAAA 
TTTGACAACC TTCTGACAGG ACCTAAAATG GATGTGCTGA AATCAGGCAT GAAGCAGGCA 
GCCACTGTAG CAAGTAAAGT TTGGGGGGCT GTTGCATCCG CTTATAGCTA CTCTGATGAT 
GAGGAGGAAA GTCAAAGTCA GAGAGGTCAG TCTAACTTTC CTGTAAGACC TCAAGAGTCT 
GGTGTTGGTG ACAATCTTTC CTTCAAGATG CCTTCAAATG GACTTTCTGG CAATGAGCTG 
ACACAGAGCA ATACCAGCTT AGGCAGCAGC AGCAGTGGAG ACACAAGCAA GCACAGCTAC 
TTAAATGAAT CTGTACAGGG GAAAGCATCA AAGGTTCTAG AGTCTGAAAA ATCAGAGCAA 
GGATCCTCTC ACAGCACCAG CTTGTCAAGC ATATTTCAGA ACTGGGCAAT GGAGGTCTTG 
ATGTCTAGTT GCTCTCAGTG CCGAGGTTGT GATGCACTTG TGTATGATGA AGAGATAATG 
GCTGGATGGA CTTTTGATGA CTCAAACTTA AATACTACTT GTCCCTTTTG TAAAAGACCC 
TTTTTACCAC TTCTTAATAT TGAGTTTAAA GACCTTCGTG GTACTGCAAG CTTTTTTCTT 
AAGCCAAGTA ACTCAGGAGA CAGTCTTCAT GGCAGTAACC TTCGGGTAAT GGCAGATTCG 
TCAGAGCGTA AACTGCAATC AGAATCTACA GCCAGTGACC TGATCAGCTT TGGGGAATCT 
TCCACTACTC CCCAAACCAC AGCAGAGGAA AGTAATACAG ACACTCAGCA GAGTGACCAG 
GCTGAAGAAG ATGGAACCAT GAAGTCTTCT GAGACAAACA ATGCACCAAA GCGAGGAGCG 
TCACTGACTA GGAGTCACAG CGTAGGAGGG CCTCTACAAA ACATTGACTT ATCCCAAAGG 
CCTTCTCATG GAATTTCCAC TGTCAGTCTT CCCAACAGCC TGCAAGAAGC TGTGGATCCT 
TTGGAAAAGC GGCACAACCC TCCTCCTGTC TCCGTCCCCT ACCTTAGTCC CCTTGTCCTA 
CGCAAGGAGC TGGAATCTTT GCTGGAGAAT GAGGGCGACC ATGTCATCTA CACATCCACT 
TTTATTAACC AGCATCCTAT TATCTTCTGG AACCTGGTTT GGTTCTTTAG ACGTTTGGAC 
CTGCCCAGCA ATTTGCCAGG GCTCATCCTC TCATCTGAGC ACTGCAATGG AGGGGTGCAG 
CTCCCGCTAT CATCTCTATC TCAGGACAGC AAGCTAGTCT ATATACAACT GTTGTGGGAC 
AACATAAACC TTCACCAGGA GCCCGGGGAG CCCCTTTACA GCAAATTTAG AGCCCTGGAC 
AATATCAAGA AGAAGGCTTC TCCTATCCCA TATGATCAAC AAAGCACCAA CAATTTACTG 
GAAAGCATTA CGCTTAGTAT CCAGCATAAT GACGTCCTTA GGCCTATCAG CCTGCTCCTG 
CAGAAATGCA GCACAACTGG AAAGAGGCAA AGGAGCTTGT ATAGAGAAAT ACTGTACCTT 
TCTTTGGTGT CTCTTGGAAG AGAAAATATC GACATAGAGG CCTTTGACAA TGAATATAGA 
ATCGCTTATG AAAAGCTCCC TCTTGAGATG CAGCAAAAAA TGCAGAAAAT TGATCAGCCT 
CCAAGCAAGA AGGTGGAATG CTGCAGGAGT ATCTTTGGAG CGCCCCTAAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910919.1
CDS	226..6099
Translation	MMEDKGPRVADYFVVAGLTDTFKLLDHDMGRIETKTLGPKAPITDIAVINKTAGETVPEGYTCIEITQSGLQANLNHGSLKSPEMFICYRREKGKPPLTDIGVLYEGKEHLLKGCEVIQATPYGRCANVNNSSGTSMRIFLTYRRSLPVRLQNSLAVTDICIIISNKGETPPHTFCKVDKNLNCGMWGSNVFLCYKKSVPASNSIPYKAGLIFRYPEEDNESFPLSESVPLFCLPMGATIECWDPQIRHPLPVFSTFVLTGSTAEKVYGAAIQFYETYPREMLSEKQMIQLGLITAVEKKVLTYKNINSKKCICLLSHWPFFETFRKFLMFIYKLSVSGPHTLPIEKHISHFMHNVPFPSPQRPRILVQLSAHDALVLSQPVATPMPLSGANFSTLLMNLGPENCASLLLFVLLECKILLHSLRPAVLTGVAEAVVAMIFPFQWQCPYIPLCPLSLADVLSAPIPFIVGVDSRYFDLYDPPQDVVCIDLDTSMVYTSEDKRNLSWKQLPKKPVKNLIGVLKKLYPRLSSVHQKSPEESAVLMAPIEADFSWQKKTTDLEMEIQEAFLRFMASILKGYRAYLKPITQAPSNKATAADSLFDRQGFLKSRDRAHIKFYTSLTKTQIFIRFIEECSFVSDKDTSLAFFDDCIDKLFPDKGVERGDKGDLEFPEDTRLIELDDSQKSEHTVFIMPPEPLPEDGPDPHPRYSYKNFPRLDYALFDRIRDQYLTFNGNTKSSRAASSPTFIAKRTKQEIKTAHKLARKYFSNDPAWGKCLFSQCFSLWFICLPVYVKVSHSKARALQKAYDVLVKMRKTDVDPLDEVCYRVVMQLCGIWENPALAVRVLFEMKNAGIQPNAITYGYYNKVVLVSPWPSSNRSGNFLWTKVRNVVFGLAQFRRATKKPQTSSESSSASALPSAAHANDGNAVSRQKVDNYNDSNSGEHTLFVRDLIRTDSTDNHSSTESPSKNGSKDKVETDDAQSGSDQKNKAETDAKAKQKHTPDCDEPSLSSMDPTVSSEGHPEKLHSTSEVPNPPESPPCASSIVKVPSGIFDSTGRKSSTGGGTGPLSPVSDVGEDHVFSSEGQNKKSSERAQKRQKMFAERSCSFSAESRAGMLLKKGSLDLTTNEIAIKMGADAKILTAALSKTPPPQHAKNEFCFESIGEQSVCQTSDSTACEQDEKPDTETEELTASRLQGPSDQLDTVKEESTVDAGDSVIGTVKDSVALRDTPKLISELNKRHSFYGLTKSVEREGVEKGLDPLSLMASQTVEKEPSPSEEKHISPVVARNLADEIESYMNLKSPLGSKSSSMELGGQECNARESGNHSQAGLERRSSLPVDSFPPSEVQSENQKNSAVSRSKTFTSSPKHHLRNQKERSVSLTALVRSSPHGSLGSMVNSFPGLKFDNLLTGPKMDVLKSGMKQAATVASKVWGAVASAYSYSDDEEESQSQRGQSNFPVRPQESGVGDNLSFKMPSNGLSGNELTQSNTSLGSSSSGDTSKHSYLNESVQGKASKVLESEKSEQGSSHSTSLSSIFQNWAMEVLMSSCSQCRGCDALVYDEEIMAGWTFDDSNLNTTCPFCKRPFLPLLNIEFKDLRGTASFFLKPSNSGDSLHGSNLRVMADSSERKLQSESTASDLISFGESSTTPQTTAEESNTDTQQSDQAEEDGTMKSSETNNAPKRGASLTRSHSVGGPLQNIDLSQRPSHGISTVSLPNSLQEAVDPLEKRHNPPPVSVPYLSPLVLRKELESLLENEGDHVIYTSTFINQHPIIFWNLVWFFRRLDLPSNLPGLILSSEHCNGGVQLPLSSLSQDSKLVYIQLLWDNINLHQEPGEPLYSKFRALDNIKKKASPIPYDQQSTNNLLESITLSIQHNDVLRPISLLLQKCSTTGKRQRSLYREILYLSLVSLGRENIDIEAFDNEYRIAYEKLPLEMQQKMQKIDQPPSKKVECCRSIFGAPLI

Target ORF information:

RefSeq Version	XM_004910862.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN/MADD domain containing 4C (dennd4c), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910862.3

ORF Insert Sequence:

ATGATGGAAG ACAAAGGGCC TCGGGTAGCA GACTACTTTG TTGTGGCAGG ACTGACAGAC 
ACCTTCAAGC TATTAGATCA TGACATGGGT CGGATAGAAA CTAAGACATT AGGTCCCAAA 
GCTCCAATCA CTGATATTGC TGTTATCAAC AAGACAGCTG GAGAGACCGT GCCAGAGGGC 
TACACATGTA TTGAAATCAC ACAAAGTGGG TTGCAGGCAA ATCTGAACCA TGGAAGCTTA 
AAGAGTCCAG AAATGTTTAT CTGTTACAGA CGAGAAAAAG GGAAACCTCC ACTAACAGAC 
ATTGGTGTGT TATATGAAGG AAAAGAACAC CTATTGAAAG GCTGTGAGGT TATCCAGGCT 
ACACCATATG GACGCTGCGC AAATGTCAAT AACAGCTCGG GTACCTCCAT GAGGATATTT 
CTAACTTACC GACGATCTTT ACCTGTTCGC CTTCAGAACT CCTTGGCAGT AACTGACATA 
TGTATAATCA TCAGTAATAA AGGAGAGACA CCACCCCATA CCTTCTGTAA GGTGGACAAA 
AACCTCAACT GTGGAATGTG GGGATCCAAT GTTTTCCTGT GTTATAAGAA GTCTGTCCCT 
GCATCTAACT CAATTCCTTA TAAAGCTGGT TTGATTTTCA GATATCCAGA GGAGGATAAC 
GAGTCATTTC CTCTATCAGA GTCCGTGCCA CTCTTTTGCC TTCCCATGGG AGCCACTATT 
GAATGCTGGG ACCCTCAAAT CCGACATCCT CTCCCTGTTT TCTCTACTTT TGTATTGACA 
GGTTCCACAG CTGAAAAGGT TTATGGTGCT GCTATTCAGT TCTATGAGAC TTACCCACGT 
GAAATGTTGT CTGAGAAGCA GATGATTCAG TTAGGACTGA TCACAGCTGT GGAGAAGAAA 
GTGCTCACCT ACAAAAATAT TAACTCCAAG AAGTGCATAT GTTTGCTTTC TCACTGGCCT 
TTCTTTGAGA CCTTCCGCAA GTTTCTTATG TTCATCTACA AGCTGTCTGT GTCTGGCCCT 
CATACCCTTC CTATTGAAAA GCACATATCG CACTTTATGC ACAATGTTCC CTTCCCATCA 
CCACAGAGGC CAAGAATTCT TGTTCAGCTC TCTGCTCATG ATGCCTTAGT TCTGTCCCAG 
CCAGTAGCAA CACCAATGCC TCTAAGTGGA GCAAATTTTA GTACACTGCT TATGAATCTT 
GGACCAGAAA ACTGTGCTTC TTTGCTGTTG TTCGTACTGC TAGAATGCAA GATTCTCCTT 
CACTCCCTCA GGCCAGCAGT GCTGACTGGA GTTGCTGAGG CTGTAGTAGC TATGATTTTC 
CCTTTCCAGT GGCAGTGCCC TTACATCCCC CTGTGCCCAC TGTCTCTCGC GGATGTGCTT 
AGTGCACCTA TTCCCTTTAT TGTGGGAGTG GATTCTCGAT ACTTTGATTT GTATGACCCG 
CCTCAGGATG TTGTTTGCAT CGACTTGGAC ACAAGCATGG TTTACACGTC TGAAGACAAG 
AGGAATTTAA GCTGGAAACA GCTTCCCAAG AAACCAGTGA AAAACCTTAT TGGCGTCTTA 
AAAAAGCTTT ACCCACGACT CTCTTCAGTT CACCAAAAGT CTCCAGAAGA GTCTGCTGTG 
CTGATGGCCC CAATTGAGGC AGACTTCTCA TGGCAAAAGA AAACAACAGA TTTGGAGATG 
GAAATTCAAG AGGCATTTCT ACGGTTCATG GCATCCATAC TCAAAGGTTA TAGGGCTTAC 
TTGAAACCCA TAACTCAAGC ACCATCCAAC AAAGCTACTG CTGCAGATTC ACTGTTTGAC 
CGTCAAGGGT TTTTGAAAAG TAGGGATCGT GCACACATAA AATTCTACAC TTCACTGACA 
AAAACCCAGA TTTTCATCCG CTTCATTGAA GAGTGCAGCT TTGTCAGTGA TAAGGACACC 
AGCCTGGCCT TCTTTGATGA CTGCATTGAT AAGTTGTTTC CTGATAAAGG AGTGGAGAGA 
GGTGATAAGG GGGATCTGGA GTTTCCAGAA GACACTCGTC TGATTGAGCT AGATGACTCA 
CAGAAGAGCG AGCACACTGT GTTTATAATG CCACCTGAGC CTCTGCCGGA AGATGGACCT 
GACCCTCACC CAAGATATAG CTACAAAAAC TTTCCAAGAC TAGACTACGC TCTTTTTGAC 
CGAATACGTG ATCAATATCT TACTTTCAAT GGGAATACTA AGAGTAGCAG AGCAGCAAGC 
AGCCCCACAT TTATTGCAAA AAGGACAAAA CAGGAAATCA AAACGGCTCA CAAGTTAGCT 
AGAAAATATT TTAGCAACGA TCCTGCTTGG GGCAAATGTC TCTTCAGCCA GTGCTTCAGT 
TTGTGGTTTA TTTGCCTGCC TGTTTATGTT AAGGTATCAC ATTCAAAAGC ACGTGCACTT 
CAGAAGGCTT ATGATGTGCT GGTTAAGATG AGGAAAACTG ATGTAGATCC ATTGGATGAG 
GTGTGTTACA GAGTGGTAAT GCAACTGTGT GGCATTTGGG AGAACCCTGC TCTGGCAGTG 
AGAGTATTAT TTGAGATGAA AAACGCTGGG ATACAGCCAA ATGCAATTAC CTATGGTTAC 
TATAATAAGG TTGTGTTGGT CAGCCCATGG CCAAGCAGTA ATCGCAGTGG AAACTTCCTA 
TGGACAAAAG TGCGCAATGT GGTGTTTGGT TTGGCTCAGT TCCGAAGGGC AACGAAGAAG 
CCACAGACAT CTTCCGAGTC CTCTTCGGCA TCAGCGCTTC CAAGTGCTGC ACATGCAAAT 
GATGGCAATG CTGTGAGCCG ACAAAAAGTG GATAATTATA ACGATTCTAA CTCTGGGGAG 
CACACCCTTT TCGTGCGCGA TCTAATCAGA ACTGATTCCA CTGATAACCA TTCTAGCACA 
GAGAGTCCAT CTAAAAATGG CTCCAAAGAC AAGGTCGAGA CAGATGATGC TCAGAGTGGT 
TCTGATCAGA AAAACAAAGC AGAAACAGAC GCTAAAGCAA AGCAGAAACA CACACCAGAC 
TGTGATGAGC CTTCACTTTC CTCTATGGAT CCAACAGTAT CTAGTGAAGG ACATCCAGAA 
AAATTACATT CAACCTCTGA GGTTCCAAAT CCTCCTGAAT CACCTCCTTG TGCAAGTAGC 
ATTGTTAAAG TACCTTCTGG AATTTTTGAC AGCACAGGAA GGAAAAGCAG TACCGGAGGT 
GGAACTGGAC CATTATCCCC GGTTTCAGAT GTTGGGGAAG ACCATGTGTT TTCCAGCGAA 
GGTCAAAACA AGAAGTCCAG TGAGAGAGCT CAAAAGAGGC AGAAAATGTT TGCTGAGCGA 
AGCTGTAGTT TCAGCGCAGA GAGCCGAGCT GGTATGCTAC TAAAGAAAGG CAGTCTTGAC 
CTAACCACTA ATGAAATTGC CATAAAGATG GGAGCGGATG CAAAAATACT TACTGCAGCT 
CTTTCAAAGA CACCACCACC CCAGCATGCT AAGAATGAAT TCTGCTTTGA AAGTATAGGT 
GAACAGTCTG TCTGTCAGAC ATCTGATAGC ACAGCTTGTG AACAAGATGA AAAACCTGAC 
ACAGAGACTG AGGAGCTAAC AGCCTCACGA CTGCAGGGGC CATCTGATCA GTTAGATACG 
GTGAAAGAGG AAAGCACAGT GGATGCTGGG GACTCTGTTA TAGGCACAGT GAAAGACAGC 
GTTGCTCTCA GGGATACTCC TAAACTTATA AGTGAACTTA ATAAAAGGCA TAGTTTTTAT 
GGCCTCACTA AATCCGTAGA AAGAGAAGGG GTAGAAAAGG GTCTTGACCC TCTATCTTTA 
ATGGCTTCAC AGACAGTGGA GAAAGAGCCA TCACCATCTG AGGAAAAGCA CATTTCCCCT 
GTTGTGGCAC GTAATCTGGC CGATGAGATT GAAAGTTACA TGAACCTGAA AAGTCCTCTT 
GGCTCCAAGT CTTCAAGTAT GGAGCTAGGA GGACAGGAGT GCAATGCAAG GGAATCTGGG 
AACCACAGCC AAGCTGGCCT GGAGCGGCGC TCTAGCTTAC CCGTAGACTC TTTTCCTCCA 
TCAGAAGTGC AGAGTGAAAA CCAGAAGAAC TCAGCTGTAT CCAGATCCAA GACTTTTACT 
AGCAGTCCAA AGCATCACCT ACGCAACCAA AAGGAACGAT CAGTTTCACT CACTGCCCTT 
GTCCGCAGCT CCCCACATGG CTCTTTGGGT TCGATGGTTA ACTCCTTTCC TGGGCTAAAA 
TTTGACAACC TTCTGACAGG ACCTAAAATG GATGTGCTGA AATCAGGCAT GAAGCAGGCA 
GCCACTGTAG CAAGTAAAGT TTGGGGGGCT GTTGCATCCG CTTATAGCTA CTCTGATGAT 
GAGGAGGAAA GTCAAAGTCA GAGAGGTCAG TCTAACTTTC CTGTAAGACC TCAAGAGTCT 
GGTGTTGGTG ACAATCTTTC CTTCAAGATG CCTTCAAATG GACTTTCTGG CAATGAGCTG 
ACACAGAGCA ATACCAGCTT AGGCAGCAGC AGCAGTGGAG ACACAAGCAA GCACAGCTAC 
TTAAATGAAT CTGTACAGGG GAAAGCATCA AAGGTTCTAG AGTCTGAAAA ATCAGAGCAA 
GGATCCTCTC ACAGCACCAG CTTGTCAAGC ATATTTCAGA ACTGGGCAAT GGAGGTCTTG 
ATGTCTAGTT GCTCTCAGTG CCGAGGTTGT GATGCACTTG TGTATGATGA AGAGATAATG 
GCTGGATGGA CTTTTGATGA CTCAAACTTA AATACTACTT GTCCCTTTTG TAAAAGACCC 
TTTTTACCAC TTCTTAATAT TGAGTTTAAA GACCTTCGTG GTACTGCAAG CTTTTTTCTT 
AAGCCAAGTA ACTCAGGAGA CAGTCTTCAT GGCAGTAACC TTCGGGTAAT GGCAGATTCG 
TCAGAGCGTA AACTGCAATC AGAATCTACA GCCAGTGACC TGATCAGCTT TGGGGAATCT 
TCCACTACTC CCCAAACCAC AGCAGAGGAA AGTAATACAG ACACTCAGCA GAGTGACCAG 
GCTGAAGAAG ATGGAACCAT GAAGTCTTCT GAGACAAACA ATGCACCAAA GCGAGGAGCG 
TCACTGACTA GGAGTCACAG CGTAGGAGGG CCTCTACAAA ACATTGACTT ATCCCAAAGG 
CCTTCTCATG GAATTTCCAC TGTCAGTCTT CCCAACAGCC TGCAAGAAGC TGTGGATCCT 
TTGGAAAAGC GGCACAACCC TCCTCCTGTC TCCGTCCCCT ACCTTAGTCC CCTTGTCCTA 
CGCAAGGAGC TGGAATCTTT GCTGGAGAAT GAGGGCGACC ATGTCATCTA CACATCCACT 
TTTATTAACC AGCATCCTAT TATCTTCTGG AACCTGGTTT GGTTCTTTAG ACGTTTGGAC 
CTGCCCAGCA ATTTGCCAGG GCTCATCCTC TCATCTGAGC ACTGCAATGG AGGGGTGCAG 
CTCCCGCTAT CATCTCTATC TCAGGACAGC AAGCTAGTCT ATATACAACT GTTGTGGGAC 
AACATAAACC TTCACCAGGA GCCCGGGGAG CCCCTTTACA GCAAATTTAG AGCCCTGGAC 
AATATCAAGA AGAAGGCTTC TCCTATCCCA TATGATCAAC AAAGCACCAA CAATTTACTG 
GAAAGCATTA CGCTTAGTAT CCAGCATAAT GACGTCCTTA GGCCTATCAG CCTGCTCCTG 
CAGAAATGCA GCACAACTGG AAAGAGGCAA AGGAGCTTGT ATAGAGAAAT ACTGTACCTT 
TCTTTGGTGT CTCTTGGAAG AGAAAATATC GACATAGAGG CCTTTGACAA TGAATATAGA 
ATCGCTTATG AAAAGCTCCC TCTTGAGATG CAGCAAAAAA TGCAGAAAAT TGATCAGCCT 
CCAAGCAAGA AGGTGGAATG CTGCAGGAGT ATCTTTGGAG CGCCCCTAAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd4c ( XM_002935667.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935713.2 Xenopus tropicalis DENN/MADD domain containing 4C (dennd4c), transcript variant X3, mRNA.

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CloneID	OXa04068
Clone ID Related Accession (Same CDS sequence)	XM_004910862.4 , XM_004910863.3 , XM_004910863.4 , XM_004910862.3 , XM_002935667.4 , XM_002935667.5
Accession Version	XM_002935667.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DENN domain-containing protein 4C isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935667.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG ACAAAGGGCC TCGGGTAGCA GACTACTTTG TTGTGGCAGG ACTGACAGAC 
ACCTTCAAGC TATTAGATCA TGACATGGGT CGGATAGAAA CTAAGACATT AGGTCCCAAA 
GCTCCAATCA CTGATATTGC TGTTATCAAC AAGACAGCTG GAGAGACCGT GCCAGAGGGC 
TACACATGTA TTGAAATCAC ACAAAGTGGG TTGCAGGCAA ATCTGAACCA TGGAAGCTTA 
AAGAGTCCAG AAATGTTTAT CTGTTACAGA CGAGAAAAAG GGAAACCTCC ACTAACAGAC 
ATTGGTGTGT TATATGAAGG AAAAGAACAC CTATTGAAAG GCTGTGAGGT TATCCAGGCT 
ACACCATATG GACGCTGCGC AAATGTCAAT AACAGCTCGG GTACCTCCAT GAGGATATTT 
CTAACTTACC GACGATCTTT ACCTGTTCGC CTTCAGAACT CCTTGGCAGT AACTGACATA 
TGTATAATCA TCAGTAATAA AGGAGAGACA CCACCCCATA CCTTCTGTAA GGTGGACAAA 
AACCTCAACT GTGGAATGTG GGGATCCAAT GTTTTCCTGT GTTATAAGAA GTCTGTCCCT 
GCATCTAACT CAATTCCTTA TAAAGCTGGT TTGATTTTCA GATATCCAGA GGAGGATAAC 
GAGTCATTTC CTCTATCAGA GTCCGTGCCA CTCTTTTGCC TTCCCATGGG AGCCACTATT 
GAATGCTGGG ACCCTCAAAT CCGACATCCT CTCCCTGTTT TCTCTACTTT TGTATTGACA 
GGTTCCACAG CTGAAAAGGT TTATGGTGCT GCTATTCAGT TCTATGAGAC TTACCCACGT 
GAAATGTTGT CTGAGAAGCA GATGATTCAG TTAGGACTGA TCACAGCTGT GGAGAAGAAA 
GTGCTCACCT ACAAAAATAT TAACTCCAAG AAGTGCATAT GTTTGCTTTC TCACTGGCCT 
TTCTTTGAGA CCTTCCGCAA GTTTCTTATG TTCATCTACA AGCTGTCTGT GTCTGGCCCT 
CATACCCTTC CTATTGAAAA GCACATATCG CACTTTATGC ACAATGTTCC CTTCCCATCA 
CCACAGAGGC CAAGAATTCT TGTTCAGCTC TCTGCTCATG ATGCCTTAGT TCTGTCCCAG 
CCAGTAGCAA CACCAATGCC TCTAAGTGGA GCAAATTTTA GTACACTGCT TATGAATCTT 
GGACCAGAAA ACTGTGCTTC TTTGCTGTTG TTCGTACTGC TAGAATGCAA GATTCTCCTT 
CACTCCCTCA GGCCAGCAGT GCTGACTGGA GTTGCTGAGG CTGTAGTAGC TATGATTTTC 
CCTTTCCAGT GGCAGTGCCC TTACATCCCC CTGTGCCCAC TGTCTCTCGC GGATGTGCTT 
AGTGCACCTA TTCCCTTTAT TGTGGGAGTG GATTCTCGAT ACTTTGATTT GTATGACCCG 
CCTCAGGATG TTGTTTGCAT CGACTTGGAC ACAAGCATGG TTTACACGTC TGAAGACAAG 
AGGAATTTAA GCTGGAAACA GCTTCCCAAG AAACCAGTGA AAAACCTTAT TGGCGTCTTA 
AAAAAGCTTT ACCCACGACT CTCTTCAGTT CACCAAAAGT CTCCAGAAGA GTCTGCTGTG 
CTGATGGCCC CAATTGAGGC AGACTTCTCA TGGCAAAAGA AAACAACAGA TTTGGAGATG 
GAAATTCAAG AGGCATTTCT ACGGTTCATG GCATCCATAC TCAAAGGTTA TAGGGCTTAC 
TTGAAACCCA TAACTCAAGC ACCATCCAAC AAAGCTACTG CTGCAGATTC ACTGTTTGAC 
CGTCAAGGGT TTTTGAAAAG TAGGGATCGT GCACACATAA AATTCTACAC TTCACTGACA 
AAAACCCAGA TTTTCATCCG CTTCATTGAA GAGTGCAGCT TTGTCAGTGA TAAGGACACC 
AGCCTGGCCT TCTTTGATGA CTGCATTGAT AAGTTGTTTC CTGATAAAGG AGTGGAGAGA 
GGTGATAAGG GGGATCTGGA GTTTCCAGAA GACACTCGTC TGATTGAGCT AGATGACTCA 
CAGAAGAGCG AGCACACTGT GTTTATAATG CCACCTGAGC CTCTGCCGGA AGATGGACCT 
GACCCTCACC CAAGATATAG CTACAAAAAC TTTCCAAGAC TAGACTACGC TCTTTTTGAC 
CGAATACGTG ATCAATATCT TACTTTCAAT GGGAATACTA AGAGTAGCAG AGCAGCAAGC 
AGCCCCACAT TTATTGCAAA AAGGACAAAA CAGGAAATCA AAACGGCTCA CAAGTTAGCT 
AGAAAATATT TTAGCAACGA TCCTGCTTGG GGCAAATGTC TCTTCAGCCA GTGCTTCAGT 
TTGTGGTTTA TTTGCCTGCC TGTTTATGTT AAGGTATCAC ATTCAAAAGC ACGTGCACTT 
CAGAAGGCTT ATGATGTGCT GGTTAAGATG AGGAAAACTG ATGTAGATCC ATTGGATGAG 
GTGTGTTACA GAGTGGTAAT GCAACTGTGT GGCATTTGGG AGAACCCTGC TCTGGCAGTG 
AGAGTATTAT TTGAGATGAA AAACGCTGGG ATACAGCCAA ATGCAATTAC CTATGGTTAC 
TATAATAAGG TTGTGTTGGT CAGCCCATGG CCAAGCAGTA ATCGCAGTGG AAACTTCCTA 
TGGACAAAAG TGCGCAATGT GGTGTTTGGT TTGGCTCAGT TCCGAAGGGC AACGAAGAAG 
CCACAGACAT CTTCCGAGTC CTCTTCGGCA TCAGCGCTTC CAAGTGCTGC ACATGCAAAT 
GATGGCAATG CTGTGAGCCG ACAAAAAGTG GATAATTATA ACGATTCTAA CTCTGGGGAG 
CACACCCTTT TCGTGCGCGA TCTAATCAGA ACTGATTCCA CTGATAACCA TTCTAGCACA 
GAGAGTCCAT CTAAAAATGG CTCCAAAGAC AAGGTCGAGA CAGATGATGC TCAGAGTGGT 
TCTGATCAGA AAAACAAAGC AGAAACAGAC GCTAAAGCAA AGCAGAAACA CACACCAGAC 
TGTGATGAGC CTTCACTTTC CTCTATGGAT CCAACAGTAT CTAGTGAAGG ACATCCAGAA 
AAATTACATT CAACCTCTGA GGTTCCAAAT CCTCCTGAAT CACCTCCTTG TGCAAGTAGC 
ATTGTTAAAG TACCTTCTGG AATTTTTGAC AGCACAGGAA GGAAAAGCAG TACCGGAGGT 
GGAACTGGAC CATTATCCCC GGTTTCAGAT GTTGGGGAAG ACCATGTGTT TTCCAGCGAA 
GGTCAAAACA AGAAGTCCAG TGAGAGAGCT CAAAAGAGGC AGAAAATGTT TGCTGAGCGA 
AGCTGTAGTT TCAGCGCAGA GAGCCGAGCT GGTATGCTAC TAAAGAAAGG CAGTCTTGAC 
CTAACCACTA ATGAAATTGC CATAAAGATG GGAGCGGATG CAAAAATACT TACTGCAGCT 
CTTTCAAAGA CACCACCACC CCAGCATGCT AAGAATGAAT TCTGCTTTGA AAGTATAGGT 
GAACAGTCTG TCTGTCAGAC ATCTGATAGC ACAGCTTGTG AACAAGATGA AAAACCTGAC 
ACAGAGACTG AGGAGCTAAC AGCCTCACGA CTGCAGGGGC CATCTGATCA GTTAGATACG 
GTGAAAGAGG AAAGCACAGT GGATGCTGGG GACTCTGTTA TAGGCACAGT GAAAGACAGC 
GTTGCTCTCA GGGATACTCC TAAACTTATA AGTGAACTTA ATAAAAGGCA TAGTTTTTAT 
GGCCTCACTA AATCCGTAGA AAGAGAAGGG GTAGAAAAGG GTCTTGACCC TCTATCTTTA 
ATGGCTTCAC AGACAGTGGA GAAAGAGCCA TCACCATCTG AGGAAAAGCA CATTTCCCCT 
GTTGTGGCAC GTAATCTGGC CGATGAGATT GAAAGTTACA TGAACCTGAA AAGTCCTCTT 
GGCTCCAAGT CTTCAAGTAT GGAGCTAGGA GGACAGGAGT GCAATGCAAG GGAATCTGGG 
AACCACAGCC AAGCTGGCCT GGAGCGGCGC TCTAGCTTAC CCGTAGACTC TTTTCCTCCA 
TCAGAAGTGC AGAGTGAAAA CCAGAAGAAC TCAGCTGTAT CCAGATCCAA GACTTTTACT 
AGCAGTCCAA AGCATCACCT ACGCAACCAA AAGGAACGAT CAGTTTCACT CACTGCCCTT 
GTCCGCAGCT CCCCACATGG CTCTTTGGGT TCGATGGTTA ACTCCTTTCC TGGGCTAAAA 
TTTGACAACC TTCTGACAGG ACCTAAAATG GATGTGCTGA AATCAGGCAT GAAGCAGGCA 
GCCACTGTAG CAAGTAAAGT TTGGGGGGCT GTTGCATCCG CTTATAGCTA CTCTGATGAT 
GAGGAGGAAA GTCAAAGTCA GAGAGGTCAG TCTAACTTTC CTGTAAGACC TCAAGAGTCT 
GGTGTTGGTG ACAATCTTTC CTTCAAGATG CCTTCAAATG GACTTTCTGG CAATGAGCTG 
ACACAGAGCA ATACCAGCTT AGGCAGCAGC AGCAGTGGAG ACACAAGCAA GCACAGCTAC 
TTAAATGAAT CTGTACAGGG GAAAGCATCA AAGGTTCTAG AGTCTGAAAA ATCAGAGCAA 
GGATCCTCTC ACAGCACCAG CTTGTCAAGC ATATTTCAGA ACTGGGCAAT GGAGGTCTTG 
ATGTCTAGTT GCTCTCAGTG CCGAGGTTGT GATGCACTTG TGTATGATGA AGAGATAATG 
GCTGGATGGA CTTTTGATGA CTCAAACTTA AATACTACTT GTCCCTTTTG TAAAAGACCC 
TTTTTACCAC TTCTTAATAT TGAGTTTAAA GACCTTCGTG GTACTGCAAG CTTTTTTCTT 
AAGCCAAGTA ACTCAGGAGA CAGTCTTCAT GGCAGTAACC TTCGGGTAAT GGCAGATTCG 
TCAGAGCGTA AACTGCAATC AGAATCTACA GCCAGTGACC TGATCAGCTT TGGGGAATCT 
TCCACTACTC CCCAAACCAC AGCAGAGGAA AGTAATACAG ACACTCAGCA GAGTGACCAG 
GCTGAAGAAG ATGGAACCAT GAAGTCTTCT GAGACAAACA ATGCACCAAA GCGAGGAGCG 
TCACTGACTA GGAGTCACAG CGTAGGAGGG CCTCTACAAA ACATTGACTT ATCCCAAAGG 
CCTTCTCATG GAATTTCCAC TGTCAGTCTT CCCAACAGCC TGCAAGAAGC TGTGGATCCT 
TTGGAAAAGC GGCACAACCC TCCTCCTGTC TCCGTCCCCT ACCTTAGTCC CCTTGTCCTA 
CGCAAGGAGC TGGAATCTTT GCTGGAGAAT GAGGGCGACC ATGTCATCTA CACATCCACT 
TTTATTAACC AGCATCCTAT TATCTTCTGG AACCTGGTTT GGTTCTTTAG ACGTTTGGAC 
CTGCCCAGCA ATTTGCCAGG GCTCATCCTC TCATCTGAGC ACTGCAATGG AGGGGTGCAG 
CTCCCGCTAT CATCTCTATC TCAGGACAGC AAGCTAGTCT ATATACAACT GTTGTGGGAC 
AACATAAACC TTCACCAGGA GCCCGGGGAG CCCCTTTACA GCAAATTTAG AGCCCTGGAC 
AATATCAAGA AGAAGGCTTC TCCTATCCCA TATGATCAAC AAAGCACCAA CAATTTACTG 
GAAAGCATTA CGCTTAGTAT CCAGCATAAT GACGTCCTTA GGCCTATCAG CCTGCTCCTG 
CAGAAATGCA GCACAACTGG AAAGAGGCAA AGGAGCTTGT ATAGAGAAAT ACTGTACCTT 
TCTTTGGTGT CTCTTGGAAG AGAAAATATC GACATAGAGG CCTTTGACAA TGAATATAGA 
ATCGCTTATG AAAAGCTCCC TCTTGAGATG CAGCAAAAAA TGCAGAAAAT TGATCAGCCT 
CCAAGCAAGA AGGTGGAATG CTGCAGGAGT ATCTTTGGAG CGCCCCTAAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935713.2
CDS	67..5940
Translation	MMEDKGPRVADYFVVAGLTDTFKLLDHDMGRIETKTLGPKAPITDIAVINKTAGETVPEGYTCIEITQSGLQANLNHGSLKSPEMFICYRREKGKPPLTDIGVLYEGKEHLLKGCEVIQATPYGRCANVNNSSGTSMRIFLTYRRSLPVRLQNSLAVTDICIIISNKGETPPHTFCKVDKNLNCGMWGSNVFLCYKKSVPASNSIPYKAGLIFRYPEEDNESFPLSESVPLFCLPMGATIECWDPQIRHPLPVFSTFVLTGSTAEKVYGAAIQFYETYPREMLSEKQMIQLGLITAVEKKVLTYKNINSKKCICLLSHWPFFETFRKFLMFIYKLSVSGPHTLPIEKHISHFMHNVPFPSPQRPRILVQLSAHDALVLSQPVATPMPLSGANFSTLLMNLGPENCASLLLFVLLECKILLHSLRPAVLTGVAEAVVAMIFPFQWQCPYIPLCPLSLADVLSAPIPFIVGVDSRYFDLYDPPQDVVCIDLDTSMVYTSEDKRNLSWKQLPKKPVKNLIGVLKKLYPRLSSVHQKSPEESAVLMAPIEADFSWQKKTTDLEMEIQEAFLRFMASILKGYRAYLKPITQAPSNKATAADSLFDRQGFLKSRDRAHIKFYTSLTKTQIFIRFIEECSFVSDKDTSLAFFDDCIDKLFPDKGVERGDKGDLEFPEDTRLIELDDSQKSEHTVFIMPPEPLPEDGPDPHPRYSYKNFPRLDYALFDRIRDQYLTFNGNTKSSRAASSPTFIAKRTKQEIKTAHKLARKYFSNDPAWGKCLFSQCFSLWFICLPVYVKVSHSKARALQKAYDVLVKMRKTDVDPLDEVCYRVVMQLCGIWENPALAVRVLFEMKNAGIQPNAITYGYYNKVVLVSPWPSSNRSGNFLWTKVRNVVFGLAQFRRATKKPQTSSESSSASALPSAAHANDGNAVSRQKVDNYNDSNSGEHTLFVRDLIRTDSTDNHSSTESPSKNGSKDKVETDDAQSGSDQKNKAETDAKAKQKHTPDCDEPSLSSMDPTVSSEGHPEKLHSTSEVPNPPESPPCASSIVKVPSGIFDSTGRKSSTGGGTGPLSPVSDVGEDHVFSSEGQNKKSSERAQKRQKMFAERSCSFSAESRAGMLLKKGSLDLTTNEIAIKMGADAKILTAALSKTPPPQHAKNEFCFESIGEQSVCQTSDSTACEQDEKPDTETEELTASRLQGPSDQLDTVKEESTVDAGDSVIGTVKDSVALRDTPKLISELNKRHSFYGLTKSVEREGVEKGLDPLSLMASQTVEKEPSPSEEKHISPVVARNLADEIESYMNLKSPLGSKSSSMELGGQECNARESGNHSQAGLERRSSLPVDSFPPSEVQSENQKNSAVSRSKTFTSSPKHHLRNQKERSVSLTALVRSSPHGSLGSMVNSFPGLKFDNLLTGPKMDVLKSGMKQAATVASKVWGAVASAYSYSDDEEESQSQRGQSNFPVRPQESGVGDNLSFKMPSNGLSGNELTQSNTSLGSSSSGDTSKHSYLNESVQGKASKVLESEKSEQGSSHSTSLSSIFQNWAMEVLMSSCSQCRGCDALVYDEEIMAGWTFDDSNLNTTCPFCKRPFLPLLNIEFKDLRGTASFFLKPSNSGDSLHGSNLRVMADSSERKLQSESTASDLISFGESSTTPQTTAEESNTDTQQSDQAEEDGTMKSSETNNAPKRGASLTRSHSVGGPLQNIDLSQRPSHGISTVSLPNSLQEAVDPLEKRHNPPPVSVPYLSPLVLRKELESLLENEGDHVIYTSTFINQHPIIFWNLVWFFRRLDLPSNLPGLILSSEHCNGGVQLPLSSLSQDSKLVYIQLLWDNINLHQEPGEPLYSKFRALDNIKKKASPIPYDQQSTNNLLESITLSIQHNDVLRPISLLLQKCSTTGKRQRSLYREILYLSLVSLGRENIDIEAFDNEYRIAYEKLPLEMQQKMQKIDQPPSKKVECCRSIFGAPLI

Target ORF information:

RefSeq Version	XM_002935667.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN/MADD domain containing 4C (dennd4c), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935667.4

ORF Insert Sequence:

ATGATGGAAG ACAAAGGGCC TCGGGTAGCA GACTACTTTG TTGTGGCAGG ACTGACAGAC 
ACCTTCAAGC TATTAGATCA TGACATGGGT CGGATAGAAA CTAAGACATT AGGTCCCAAA 
GCTCCAATCA CTGATATTGC TGTTATCAAC AAGACAGCTG GAGAGACCGT GCCAGAGGGC 
TACACATGTA TTGAAATCAC ACAAAGTGGG TTGCAGGCAA ATCTGAACCA TGGAAGCTTA 
AAGAGTCCAG AAATGTTTAT CTGTTACAGA CGAGAAAAAG GGAAACCTCC ACTAACAGAC 
ATTGGTGTGT TATATGAAGG AAAAGAACAC CTATTGAAAG GCTGTGAGGT TATCCAGGCT 
ACACCATATG GACGCTGCGC AAATGTCAAT AACAGCTCGG GTACCTCCAT GAGGATATTT 
CTAACTTACC GACGATCTTT ACCTGTTCGC CTTCAGAACT CCTTGGCAGT AACTGACATA 
TGTATAATCA TCAGTAATAA AGGAGAGACA CCACCCCATA CCTTCTGTAA GGTGGACAAA 
AACCTCAACT GTGGAATGTG GGGATCCAAT GTTTTCCTGT GTTATAAGAA GTCTGTCCCT 
GCATCTAACT CAATTCCTTA TAAAGCTGGT TTGATTTTCA GATATCCAGA GGAGGATAAC 
GAGTCATTTC CTCTATCAGA GTCCGTGCCA CTCTTTTGCC TTCCCATGGG AGCCACTATT 
GAATGCTGGG ACCCTCAAAT CCGACATCCT CTCCCTGTTT TCTCTACTTT TGTATTGACA 
GGTTCCACAG CTGAAAAGGT TTATGGTGCT GCTATTCAGT TCTATGAGAC TTACCCACGT 
GAAATGTTGT CTGAGAAGCA GATGATTCAG TTAGGACTGA TCACAGCTGT GGAGAAGAAA 
GTGCTCACCT ACAAAAATAT TAACTCCAAG AAGTGCATAT GTTTGCTTTC TCACTGGCCT 
TTCTTTGAGA CCTTCCGCAA GTTTCTTATG TTCATCTACA AGCTGTCTGT GTCTGGCCCT 
CATACCCTTC CTATTGAAAA GCACATATCG CACTTTATGC ACAATGTTCC CTTCCCATCA 
CCACAGAGGC CAAGAATTCT TGTTCAGCTC TCTGCTCATG ATGCCTTAGT TCTGTCCCAG 
CCAGTAGCAA CACCAATGCC TCTAAGTGGA GCAAATTTTA GTACACTGCT TATGAATCTT 
GGACCAGAAA ACTGTGCTTC TTTGCTGTTG TTCGTACTGC TAGAATGCAA GATTCTCCTT 
CACTCCCTCA GGCCAGCAGT GCTGACTGGA GTTGCTGAGG CTGTAGTAGC TATGATTTTC 
CCTTTCCAGT GGCAGTGCCC TTACATCCCC CTGTGCCCAC TGTCTCTCGC GGATGTGCTT 
AGTGCACCTA TTCCCTTTAT TGTGGGAGTG GATTCTCGAT ACTTTGATTT GTATGACCCG 
CCTCAGGATG TTGTTTGCAT CGACTTGGAC ACAAGCATGG TTTACACGTC TGAAGACAAG 
AGGAATTTAA GCTGGAAACA GCTTCCCAAG AAACCAGTGA AAAACCTTAT TGGCGTCTTA 
AAAAAGCTTT ACCCACGACT CTCTTCAGTT CACCAAAAGT CTCCAGAAGA GTCTGCTGTG 
CTGATGGCCC CAATTGAGGC AGACTTCTCA TGGCAAAAGA AAACAACAGA TTTGGAGATG 
GAAATTCAAG AGGCATTTCT ACGGTTCATG GCATCCATAC TCAAAGGTTA TAGGGCTTAC 
TTGAAACCCA TAACTCAAGC ACCATCCAAC AAAGCTACTG CTGCAGATTC ACTGTTTGAC 
CGTCAAGGGT TTTTGAAAAG TAGGGATCGT GCACACATAA AATTCTACAC TTCACTGACA 
AAAACCCAGA TTTTCATCCG CTTCATTGAA GAGTGCAGCT TTGTCAGTGA TAAGGACACC 
AGCCTGGCCT TCTTTGATGA CTGCATTGAT AAGTTGTTTC CTGATAAAGG AGTGGAGAGA 
GGTGATAAGG GGGATCTGGA GTTTCCAGAA GACACTCGTC TGATTGAGCT AGATGACTCA 
CAGAAGAGCG AGCACACTGT GTTTATAATG CCACCTGAGC CTCTGCCGGA AGATGGACCT 
GACCCTCACC CAAGATATAG CTACAAAAAC TTTCCAAGAC TAGACTACGC TCTTTTTGAC 
CGAATACGTG ATCAATATCT TACTTTCAAT GGGAATACTA AGAGTAGCAG AGCAGCAAGC 
AGCCCCACAT TTATTGCAAA AAGGACAAAA CAGGAAATCA AAACGGCTCA CAAGTTAGCT 
AGAAAATATT TTAGCAACGA TCCTGCTTGG GGCAAATGTC TCTTCAGCCA GTGCTTCAGT 
TTGTGGTTTA TTTGCCTGCC TGTTTATGTT AAGGTATCAC ATTCAAAAGC ACGTGCACTT 
CAGAAGGCTT ATGATGTGCT GGTTAAGATG AGGAAAACTG ATGTAGATCC ATTGGATGAG 
GTGTGTTACA GAGTGGTAAT GCAACTGTGT GGCATTTGGG AGAACCCTGC TCTGGCAGTG 
AGAGTATTAT TTGAGATGAA AAACGCTGGG ATACAGCCAA ATGCAATTAC CTATGGTTAC 
TATAATAAGG TTGTGTTGGT CAGCCCATGG CCAAGCAGTA ATCGCAGTGG AAACTTCCTA 
TGGACAAAAG TGCGCAATGT GGTGTTTGGT TTGGCTCAGT TCCGAAGGGC AACGAAGAAG 
CCACAGACAT CTTCCGAGTC CTCTTCGGCA TCAGCGCTTC CAAGTGCTGC ACATGCAAAT 
GATGGCAATG CTGTGAGCCG ACAAAAAGTG GATAATTATA ACGATTCTAA CTCTGGGGAG 
CACACCCTTT TCGTGCGCGA TCTAATCAGA ACTGATTCCA CTGATAACCA TTCTAGCACA 
GAGAGTCCAT CTAAAAATGG CTCCAAAGAC AAGGTCGAGA CAGATGATGC TCAGAGTGGT 
TCTGATCAGA AAAACAAAGC AGAAACAGAC GCTAAAGCAA AGCAGAAACA CACACCAGAC 
TGTGATGAGC CTTCACTTTC CTCTATGGAT CCAACAGTAT CTAGTGAAGG ACATCCAGAA 
AAATTACATT CAACCTCTGA GGTTCCAAAT CCTCCTGAAT CACCTCCTTG TGCAAGTAGC 
ATTGTTAAAG TACCTTCTGG AATTTTTGAC AGCACAGGAA GGAAAAGCAG TACCGGAGGT 
GGAACTGGAC CATTATCCCC GGTTTCAGAT GTTGGGGAAG ACCATGTGTT TTCCAGCGAA 
GGTCAAAACA AGAAGTCCAG TGAGAGAGCT CAAAAGAGGC AGAAAATGTT TGCTGAGCGA 
AGCTGTAGTT TCAGCGCAGA GAGCCGAGCT GGTATGCTAC TAAAGAAAGG CAGTCTTGAC 
CTAACCACTA ATGAAATTGC CATAAAGATG GGAGCGGATG CAAAAATACT TACTGCAGCT 
CTTTCAAAGA CACCACCACC CCAGCATGCT AAGAATGAAT TCTGCTTTGA AAGTATAGGT 
GAACAGTCTG TCTGTCAGAC ATCTGATAGC ACAGCTTGTG AACAAGATGA AAAACCTGAC 
ACAGAGACTG AGGAGCTAAC AGCCTCACGA CTGCAGGGGC CATCTGATCA GTTAGATACG 
GTGAAAGAGG AAAGCACAGT GGATGCTGGG GACTCTGTTA TAGGCACAGT GAAAGACAGC 
GTTGCTCTCA GGGATACTCC TAAACTTATA AGTGAACTTA ATAAAAGGCA TAGTTTTTAT 
GGCCTCACTA AATCCGTAGA AAGAGAAGGG GTAGAAAAGG GTCTTGACCC TCTATCTTTA 
ATGGCTTCAC AGACAGTGGA GAAAGAGCCA TCACCATCTG AGGAAAAGCA CATTTCCCCT 
GTTGTGGCAC GTAATCTGGC CGATGAGATT GAAAGTTACA TGAACCTGAA AAGTCCTCTT 
GGCTCCAAGT CTTCAAGTAT GGAGCTAGGA GGACAGGAGT GCAATGCAAG GGAATCTGGG 
AACCACAGCC AAGCTGGCCT GGAGCGGCGC TCTAGCTTAC CCGTAGACTC TTTTCCTCCA 
TCAGAAGTGC AGAGTGAAAA CCAGAAGAAC TCAGCTGTAT CCAGATCCAA GACTTTTACT 
AGCAGTCCAA AGCATCACCT ACGCAACCAA AAGGAACGAT CAGTTTCACT CACTGCCCTT 
GTCCGCAGCT CCCCACATGG CTCTTTGGGT TCGATGGTTA ACTCCTTTCC TGGGCTAAAA 
TTTGACAACC TTCTGACAGG ACCTAAAATG GATGTGCTGA AATCAGGCAT GAAGCAGGCA 
GCCACTGTAG CAAGTAAAGT TTGGGGGGCT GTTGCATCCG CTTATAGCTA CTCTGATGAT 
GAGGAGGAAA GTCAAAGTCA GAGAGGTCAG TCTAACTTTC CTGTAAGACC TCAAGAGTCT 
GGTGTTGGTG ACAATCTTTC CTTCAAGATG CCTTCAAATG GACTTTCTGG CAATGAGCTG 
ACACAGAGCA ATACCAGCTT AGGCAGCAGC AGCAGTGGAG ACACAAGCAA GCACAGCTAC 
TTAAATGAAT CTGTACAGGG GAAAGCATCA AAGGTTCTAG AGTCTGAAAA ATCAGAGCAA 
GGATCCTCTC ACAGCACCAG CTTGTCAAGC ATATTTCAGA ACTGGGCAAT GGAGGTCTTG 
ATGTCTAGTT GCTCTCAGTG CCGAGGTTGT GATGCACTTG TGTATGATGA AGAGATAATG 
GCTGGATGGA CTTTTGATGA CTCAAACTTA AATACTACTT GTCCCTTTTG TAAAAGACCC 
TTTTTACCAC TTCTTAATAT TGAGTTTAAA GACCTTCGTG GTACTGCAAG CTTTTTTCTT 
AAGCCAAGTA ACTCAGGAGA CAGTCTTCAT GGCAGTAACC TTCGGGTAAT GGCAGATTCG 
TCAGAGCGTA AACTGCAATC AGAATCTACA GCCAGTGACC TGATCAGCTT TGGGGAATCT 
TCCACTACTC CCCAAACCAC AGCAGAGGAA AGTAATACAG ACACTCAGCA GAGTGACCAG 
GCTGAAGAAG ATGGAACCAT GAAGTCTTCT GAGACAAACA ATGCACCAAA GCGAGGAGCG 
TCACTGACTA GGAGTCACAG CGTAGGAGGG CCTCTACAAA ACATTGACTT ATCCCAAAGG 
CCTTCTCATG GAATTTCCAC TGTCAGTCTT CCCAACAGCC TGCAAGAAGC TGTGGATCCT 
TTGGAAAAGC GGCACAACCC TCCTCCTGTC TCCGTCCCCT ACCTTAGTCC CCTTGTCCTA 
CGCAAGGAGC TGGAATCTTT GCTGGAGAAT GAGGGCGACC ATGTCATCTA CACATCCACT 
TTTATTAACC AGCATCCTAT TATCTTCTGG AACCTGGTTT GGTTCTTTAG ACGTTTGGAC 
CTGCCCAGCA ATTTGCCAGG GCTCATCCTC TCATCTGAGC ACTGCAATGG AGGGGTGCAG 
CTCCCGCTAT CATCTCTATC TCAGGACAGC AAGCTAGTCT ATATACAACT GTTGTGGGAC 
AACATAAACC TTCACCAGGA GCCCGGGGAG CCCCTTTACA GCAAATTTAG AGCCCTGGAC 
AATATCAAGA AGAAGGCTTC TCCTATCCCA TATGATCAAC AAAGCACCAA CAATTTACTG 
GAAAGCATTA CGCTTAGTAT CCAGCATAAT GACGTCCTTA GGCCTATCAG CCTGCTCCTG 
CAGAAATGCA GCACAACTGG AAAGAGGCAA AGGAGCTTGT ATAGAGAAAT ACTGTACCTT 
TCTTTGGTGT CTCTTGGAAG AGAAAATATC GACATAGAGG CCTTTGACAA TGAATATAGA 
ATCGCTTATG AAAAGCTCCC TCTTGAGATG CAGCAAAAAA TGCAGAAAAT TGATCAGCCT 
CCAAGCAAGA AGGTGGAATG CTGCAGGAGT ATCTTTGGAG CGCCCCTAAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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dennd4c cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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