dse cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dse
Entrez Gene ID	111587140
Full Name	dermatan sulfate epimerase
Gene Type	protein-coding
Organism	Amphiprion ocellaris(clown anemonefish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023296974.1	XP_023152742.1	dermatan-sulfate epimerase
XM_023296973.1	XP_023152741.1	dermatan-sulfate epimerase

Pathways from BioSystems
Gene Ontology

The following dse gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dse cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm98547	XM_023296973.1 Latest version!	Amphiprion ocellaris dermatan sulfate epimerase (dse), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAm98547	XM_023296974.1 Latest version!	Amphiprion ocellaris dermatan sulfate epimerase (dse), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dse ( XM_023296974.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023152742.1 Amphiprion ocellaris dermatan sulfate epimerase (dse), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAm98547
Clone ID Related Accession (Same CDS sequence)	XM_023296974.1 , XM_023296973.1
Accession Version	XM_023296974.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	dermatan-sulfate epimerase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019375517.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACCC ACACCCGCGG GGCGCCCACA GTCTTCTTCA TAAGTCTGCT GTGGCCCCTG 
CTTGGTCCGG CGGCCACGGC AGATGTGGAC CCCGGCGGAG GAATCCCCTT CATGGGAGGC 
AACTACGATG GTCACCCGAT GCTGTACTTT GGTCGCGGCG ACGTGGAGGA GCTGCAGTAC 
GCCGCGGCAG GGACTCACCG CGATATGGCG AGGAGGATCC GCGAGGCTGG GGAAGCCATG 
CTGGAGCACC CAGAGGAGTA CCTGCCCCCC TGGAGCCCCG CTGAGTTCAG TGCCCGCTGG 
AACGAGGTTT ATGGGAACAA CCTGGGAGTG CTGTCCATGT TCTGTTTGCT GTACCCGCAC 
CGGGCCGGAG CCCTTGACCT GGCCAAAGAC TACATGGAGA GGATGGCAGC TCAGCCTAGT 
TGGCTGGTCA AAGACGCCCC CTGGGATGAA GTTCCTCTGG CGCACTCTTT GGTCGGGTTT 
GCCACCGCTT ATGACTTCCT GTACGAGTAC TTGAACAAGG GCCAGCAGGA GCGTTTCCTG 
CAGGTGATCG GCAACGCGTC ACGCCTGATG TACGAGAAGT CCTACGTCCG GGGTTGGGGG 
TTCCAGTACC TGCACAACCA CCAGCCCACC AACTGTGTGG CTCTGCTCAC AGGAAGTCTG 
GTTTACATGA CCCAAGGTTA CCTTCAGGAG GCCTACCTGT GGACCAAGCA GGTCCTGTCC 
ATCATGGAGA AGTTCATGGT CCTTCTGCAG GATGTGACAG ACGGCTCCCT GTATGAAGGC 
GTGGCCTACG GGACATACAC CACCCGTTCT CTGTTTCAGT ACATGTTTCT GGTGCAGCGC 
CACTTTGCCA TCAGCCACTT CGACCACCCC TGGCTCCTAA AACACTTTGC TTTCCTGTAC 
AGGACCATCC TGCCTGGATT TCAGCGAACT GTAGGTATCG CAGATTCCAA CTACAACTGG 
TTCTACGGAC CAGAGAGCCA GCTGGTCTTC TTGGACCGTT ACGTGTTGCG TAATGGCAGT 
GGAAACTGGC TGGCAGACCT GATTCATCAA AACAGAGTGA TTGAAGGGCC AGGGCAGCCT 
GGTAAAGGCC AGCGATGGTG TACTCTACAC ACAGAGTTCA TCTGGTATGA TCCAGGCCTG 
ATCCCCAAAC CCCCGTCAGA TTTTGGAACG TCGCAGCTCC ACTACTTCGA GGACTGGGGC 
GTGGTCACAT ATGGCAGCAC TCTACCTGCC AACACCAACT GTACCTTTCT GTCCTTCAAG 
TCGGGGAAGC TCGGGGGACG CGCTATCTTC GACATCGTTC ACAGGAACAA GTACAAGGAG 
TGGATCAAAG GCTGGAGGAA CTTTAACGCT GGCCACGAGC ACCCCGATCA GAACACGTTC 
ACCTTTGCAC CCAACGGCGT CCCGTTCATT ACTGAAGGAC TGTACGGACC CAAGTACACT 
TTGTTGAACA ATGCGGTGTT GTTTGGCTCG GCGTTCTCAG GGAGCTGCTT TAAGCCGTGG 
GCGGGACAAG TTACAGAAGC CTGCGACTCC AAGTGGTTGA AATACAAGGT GGGGCTTGCA 
GCTGATGCTC AGGGCAGAGT GGAAGCTGCT ATGGAGAGAG AGGGAATGGT CTTCATCCGA 
GGCGAGGGAC ATTCCGCTTA TAATCCAGAA CTGAAAATCA GGAACTTCCA GAGGAACCTG 
CTCCTTCTTC ACCCGCAGCT CCTGCTCCTC GTGGACCACA TCCACCTGGA TCCAGACAGC 
CCAGTCAGGG CCATGAGTGC CTTCTTTCAC AACACGGAGC TGCCGTTCCA AAGCACAAAG 
ATAGATGGTG TTCATGGAGC GTTTGTGTCA CATAAAGAGG ATAAATATAA GATGTTCTGG 
ATGGATGACA CCAGCTACAG TAACAAAGGG GTGTTGGGTT ACTGGAATTA CCCTCGAGGG 
TACCCCTATA ATGGCTCTAA CTATGTGAAC GTCACAATGC CGTTGAGGTA TCCTCACACC 
AGGGTGGCGT ACATTTTCTT TGGACCAGGT GTGGACGTGC AGAGCTTCAG CCTGCGTAGC 
GATGATCAGA GGGTGGATAT TTACCTGTTC ACCAAGGACC ACACTTACAC CATCTACCTG 
CTGACCGGAG AGGTCACCAG CAAGCCGCTG TTCTCCATGG TGCTACTTGA CCACAAGAAA 
ATTGTATTCG AGAAGTCAGC GGCTGTGGTA GACAGCTCGC CTGAGGAAGT GGAGGAATAC 
GTCAACGTGA TGGAGGACAA CCTCCAGCAT GTCAAACCCG TCTTCCAGCA GATGGAGAGG 
CACATCCTGG GACGGGTTCT CAACACTGCC AGCTTCCGTA AGACTGCAGA GCGCCTCCTC 
CAGTTCTCCG ACAAGAAGAA GACGGAGGAG GTCATTGAGA AGATGTTCGC CATGTCCAAG 
AAACAGAGCA AAGGCAAAGG GGGGAAGAAA GTGAACCTCG GGGAAAAGCT TTCTGAGAAT 
CTGCCTGACA TTTTTGCACA GATTGAGGTG AATGAGAAGA AGGAGAGACA GAGGACTTCA 
AAGAGAACTT ATGAGGACAG TCCGGAGGAG GGGGACGCAG ACTCACGGGC CTTTATGGAT 
TATACAGATG GCCGCAAGAA TAGAAAGGGC GGCTTTGTCA AGGGCCGCAA ATTCAAAGAG 
GTTCACATGG TGGCCACAGC AGGGAGCGAG AGTCTCCCCA GCACAGCTTC CTACATTAGA 
CTGTTCCTCC TCCTGAACAC TGCCACCTTC TTTTTACTGC TAGCTGTGCT ACTGACTCGC 
TTTCAGAGGG GTCGGAGTCT GCACACTCAG AGATGTTTCT ACACCATCCT CCTGATCGAC 
TGCTTCATCC TACTGTACCT CTACTCCTCC TGCTCTCACA GTCAGTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023152742.1
CDS	164..3034
Translation	MRTHTRGAPTVFFISLLWPLLGPAATADVDPGGGIPFMGGNYDGHPMLYFGRGDVEELQYAAAGTHRDMARRIREAGEAMLEHPEEYLPPWSPAEFSARWNEVYGNNLGVLSMFCLLYPHRAGALDLAKDYMERMAAQPSWLVKDAPWDEVPLAHSLVGFATAYDFLYEYLNKGQQERFLQVIGNASRLMYEKSYVRGWGFQYLHNHQPTNCVALLTGSLVYMTQGYLQEAYLWTKQVLSIMEKFMVLLQDVTDGSLYEGVAYGTYTTRSLFQYMFLVQRHFAISHFDHPWLLKHFAFLYRTILPGFQRTVGIADSNYNWFYGPESQLVFLDRYVLRNGSGNWLADLIHQNRVIEGPGQPGKGQRWCTLHTEFIWYDPGLIPKPPSDFGTSQLHYFEDWGVVTYGSTLPANTNCTFLSFKSGKLGGRAIFDIVHRNKYKEWIKGWRNFNAGHEHPDQNTFTFAPNGVPFITEGLYGPKYTLLNNAVLFGSAFSGSCFKPWAGQVTEACDSKWLKYKVGLAADAQGRVEAAMEREGMVFIRGEGHSAYNPELKIRNFQRNLLLLHPQLLLLVDHIHLDPDSPVRAMSAFFHNTELPFQSTKIDGVHGAFVSHKEDKYKMFWMDDTSYSNKGVLGYWNYPRGYPYNGSNYVNVTMPLRYPHTRVAYIFFGPGVDVQSFSLRSDDQRVDIYLFTKDHTYTIYLLTGEVTSKPLFSMVLLDHKKIVFEKSAAVVDSSPEEVEEYVNVMEDNLQHVKPVFQQMERHILGRVLNTASFRKTAERLLQFSDKKKTEEVIEKMFAMSKKQSKGKGGKKVNLGEKLSENLPDIFAQIEVNEKKERQRTSKRTYEDSPEEGDADSRAFMDYTDGRKNRKGGFVKGRKFKEVHMVATAGSESLPSTASYIRLFLLLNTATFFLLLAVLLTRFQRGRSLHTQRCFYTILLIDCFILLYLYSSCSHSQC

Target ORF information:

RefSeq Version	XM_023296974.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris dermatan sulfate epimerase (dse), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023296974.1

ORF Insert Sequence:

ATGAGAACCC ACACCCGCGG GGCGCCCACA GTCTTCTTCA TAAGTCTGCT GTGGCCCCTG 
CTTGGTCCGG CGGCCACGGC AGATGTGGAC CCCGGCGGAG GAATCCCCTT CATGGGAGGC 
AACTACGATG GTCACCCGAT GCTGTACTTT GGTCGCGGCG ACGTGGAGGA GCTGCAGTAC 
GCCGCGGCAG GGACTCACCG CGATATGGCG AGGAGGATCC GCGAGGCTGG GGAAGCCATG 
CTGGAGCACC CAGAGGAGTA CCTGCCCCCC TGGAGCCCCG CTGAGTTCAG TGCCCGCTGG 
AACGAGGTTT ATGGGAACAA CCTGGGAGTG CTGTCCATGT TCTGTTTGCT GTACCCGCAC 
CGGGCCGGAG CCCTTGACCT GGCCAAAGAC TACATGGAGA GGATGGCAGC TCAGCCTAGT 
TGGCTGGTCA AAGACGCCCC CTGGGATGAA GTTCCTCTGG CGCACTCTTT GGTCGGGTTT 
GCCACCGCTT ATGACTTCCT GTACGAGTAC TTGAACAAGG GCCAGCAGGA GCGTTTCCTG 
CAGGTGATCG GCAACGCGTC ACGCCTGATG TACGAGAAGT CCTACGTCCG GGGTTGGGGG 
TTCCAGTACC TGCACAACCA CCAGCCCACC AACTGTGTGG CTCTGCTCAC AGGAAGTCTG 
GTTTACATGA CCCAAGGTTA CCTTCAGGAG GCCTACCTGT GGACCAAGCA GGTCCTGTCC 
ATCATGGAGA AGTTCATGGT CCTTCTGCAG GATGTGACAG ACGGCTCCCT GTATGAAGGC 
GTGGCCTACG GGACATACAC CACCCGTTCT CTGTTTCAGT ACATGTTTCT GGTGCAGCGC 
CACTTTGCCA TCAGCCACTT CGACCACCCC TGGCTCCTAA AACACTTTGC TTTCCTGTAC 
AGGACCATCC TGCCTGGATT TCAGCGAACT GTAGGTATCG CAGATTCCAA CTACAACTGG 
TTCTACGGAC CAGAGAGCCA GCTGGTCTTC TTGGACCGTT ACGTGTTGCG TAATGGCAGT 
GGAAACTGGC TGGCAGACCT GATTCATCAA AACAGAGTGA TTGAAGGGCC AGGGCAGCCT 
GGTAAAGGCC AGCGATGGTG TACTCTACAC ACAGAGTTCA TCTGGTATGA TCCAGGCCTG 
ATCCCCAAAC CCCCGTCAGA TTTTGGAACG TCGCAGCTCC ACTACTTCGA GGACTGGGGC 
GTGGTCACAT ATGGCAGCAC TCTACCTGCC AACACCAACT GTACCTTTCT GTCCTTCAAG 
TCGGGGAAGC TCGGGGGACG CGCTATCTTC GACATCGTTC ACAGGAACAA GTACAAGGAG 
TGGATCAAAG GCTGGAGGAA CTTTAACGCT GGCCACGAGC ACCCCGATCA GAACACGTTC 
ACCTTTGCAC CCAACGGCGT CCCGTTCATT ACTGAAGGAC TGTACGGACC CAAGTACACT 
TTGTTGAACA ATGCGGTGTT GTTTGGCTCG GCGTTCTCAG GGAGCTGCTT TAAGCCGTGG 
GCGGGACAAG TTACAGAAGC CTGCGACTCC AAGTGGTTGA AATACAAGGT GGGGCTTGCA 
GCTGATGCTC AGGGCAGAGT GGAAGCTGCT ATGGAGAGAG AGGGAATGGT CTTCATCCGA 
GGCGAGGGAC ATTCCGCTTA TAATCCAGAA CTGAAAATCA GGAACTTCCA GAGGAACCTG 
CTCCTTCTTC ACCCGCAGCT CCTGCTCCTC GTGGACCACA TCCACCTGGA TCCAGACAGC 
CCAGTCAGGG CCATGAGTGC CTTCTTTCAC AACACGGAGC TGCCGTTCCA AAGCACAAAG 
ATAGATGGTG TTCATGGAGC GTTTGTGTCA CATAAAGAGG ATAAATATAA GATGTTCTGG 
ATGGATGACA CCAGCTACAG TAACAAAGGG GTGTTGGGTT ACTGGAATTA CCCTCGAGGG 
TACCCCTATA ATGGCTCTAA CTATGTGAAC GTCACAATGC CGTTGAGGTA TCCTCACACC 
AGGGTGGCGT ACATTTTCTT TGGACCAGGT GTGGACGTGC AGAGCTTCAG CCTGCGTAGC 
GATGATCAGA GGGTGGATAT TTACCTGTTC ACCAAGGACC ACACTTACAC CATCTACCTG 
CTGACCGGAG AGGTCACCAG CAAGCCGCTG TTCTCCATGG TGCTACTTGA CCACAAGAAA 
ATTGTATTCG AGAAGTCAGC GGCTGTGGTA GACAGCTCGC CTGAGGAAGT GGAGGAATAC 
GTCAACGTGA TGGAGGACAA CCTCCAGCAT GTCAAACCCG TCTTCCAGCA GATGGAGAGG 
CACATCCTGG GACGGGTTCT CAACACTGCC AGCTTCCGTA AGACTGCAGA GCGCCTCCTC 
CAGTTCTCCG ACAAGAAGAA GACGGAGGAG GTCATTGAGA AGATGTTCGC CATGTCCAAG 
AAACAGAGCA AAGGCAAAGG GGGGAAGAAA GTGAACCTCG GGGAAAAGCT TTCTGAGAAT 
CTGCCTGACA TTTTTGCACA GATTGAGGTG AATGAGAAGA AGGAGAGACA GAGGACTTCA 
AAGAGAACTT ATGAGGACAG TCCGGAGGAG GGGGACGCAG ACTCACGGGC CTTTATGGAT 
TATACAGATG GCCGCAAGAA TAGAAAGGGC GGCTTTGTCA AGGGCCGCAA ATTCAAAGAG 
GTTCACATGG TGGCCACAGC AGGGAGCGAG AGTCTCCCCA GCACAGCTTC CTACATTAGA 
CTGTTCCTCC TCCTGAACAC TGCCACCTTC TTTTTACTGC TAGCTGTGCT ACTGACTCGC 
TTTCAGAGGG GTCGGAGTCT GCACACTCAG AGATGTTTCT ACACCATCCT CCTGATCGAC 
TGCTTCATCC TACTGTACCT CTACTCCTCC TGCTCTCACA GTCAGTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dse ( XM_023296973.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023152741.1 Amphiprion ocellaris dermatan sulfate epimerase (dse), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAm98547
Clone ID Related Accession (Same CDS sequence)	XM_023296974.1 , XM_023296973.1
Accession Version	XM_023296973.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	dermatan-sulfate epimerase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019375517.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACCC ACACCCGCGG GGCGCCCACA GTCTTCTTCA TAAGTCTGCT GTGGCCCCTG 
CTTGGTCCGG CGGCCACGGC AGATGTGGAC CCCGGCGGAG GAATCCCCTT CATGGGAGGC 
AACTACGATG GTCACCCGAT GCTGTACTTT GGTCGCGGCG ACGTGGAGGA GCTGCAGTAC 
GCCGCGGCAG GGACTCACCG CGATATGGCG AGGAGGATCC GCGAGGCTGG GGAAGCCATG 
CTGGAGCACC CAGAGGAGTA CCTGCCCCCC TGGAGCCCCG CTGAGTTCAG TGCCCGCTGG 
AACGAGGTTT ATGGGAACAA CCTGGGAGTG CTGTCCATGT TCTGTTTGCT GTACCCGCAC 
CGGGCCGGAG CCCTTGACCT GGCCAAAGAC TACATGGAGA GGATGGCAGC TCAGCCTAGT 
TGGCTGGTCA AAGACGCCCC CTGGGATGAA GTTCCTCTGG CGCACTCTTT GGTCGGGTTT 
GCCACCGCTT ATGACTTCCT GTACGAGTAC TTGAACAAGG GCCAGCAGGA GCGTTTCCTG 
CAGGTGATCG GCAACGCGTC ACGCCTGATG TACGAGAAGT CCTACGTCCG GGGTTGGGGG 
TTCCAGTACC TGCACAACCA CCAGCCCACC AACTGTGTGG CTCTGCTCAC AGGAAGTCTG 
GTTTACATGA CCCAAGGTTA CCTTCAGGAG GCCTACCTGT GGACCAAGCA GGTCCTGTCC 
ATCATGGAGA AGTTCATGGT CCTTCTGCAG GATGTGACAG ACGGCTCCCT GTATGAAGGC 
GTGGCCTACG GGACATACAC CACCCGTTCT CTGTTTCAGT ACATGTTTCT GGTGCAGCGC 
CACTTTGCCA TCAGCCACTT CGACCACCCC TGGCTCCTAA AACACTTTGC TTTCCTGTAC 
AGGACCATCC TGCCTGGATT TCAGCGAACT GTAGGTATCG CAGATTCCAA CTACAACTGG 
TTCTACGGAC CAGAGAGCCA GCTGGTCTTC TTGGACCGTT ACGTGTTGCG TAATGGCAGT 
GGAAACTGGC TGGCAGACCT GATTCATCAA AACAGAGTGA TTGAAGGGCC AGGGCAGCCT 
GGTAAAGGCC AGCGATGGTG TACTCTACAC ACAGAGTTCA TCTGGTATGA TCCAGGCCTG 
ATCCCCAAAC CCCCGTCAGA TTTTGGAACG TCGCAGCTCC ACTACTTCGA GGACTGGGGC 
GTGGTCACAT ATGGCAGCAC TCTACCTGCC AACACCAACT GTACCTTTCT GTCCTTCAAG 
TCGGGGAAGC TCGGGGGACG CGCTATCTTC GACATCGTTC ACAGGAACAA GTACAAGGAG 
TGGATCAAAG GCTGGAGGAA CTTTAACGCT GGCCACGAGC ACCCCGATCA GAACACGTTC 
ACCTTTGCAC CCAACGGCGT CCCGTTCATT ACTGAAGGAC TGTACGGACC CAAGTACACT 
TTGTTGAACA ATGCGGTGTT GTTTGGCTCG GCGTTCTCAG GGAGCTGCTT TAAGCCGTGG 
GCGGGACAAG TTACAGAAGC CTGCGACTCC AAGTGGTTGA AATACAAGGT GGGGCTTGCA 
GCTGATGCTC AGGGCAGAGT GGAAGCTGCT ATGGAGAGAG AGGGAATGGT CTTCATCCGA 
GGCGAGGGAC ATTCCGCTTA TAATCCAGAA CTGAAAATCA GGAACTTCCA GAGGAACCTG 
CTCCTTCTTC ACCCGCAGCT CCTGCTCCTC GTGGACCACA TCCACCTGGA TCCAGACAGC 
CCAGTCAGGG CCATGAGTGC CTTCTTTCAC AACACGGAGC TGCCGTTCCA AAGCACAAAG 
ATAGATGGTG TTCATGGAGC GTTTGTGTCA CATAAAGAGG ATAAATATAA GATGTTCTGG 
ATGGATGACA CCAGCTACAG TAACAAAGGG GTGTTGGGTT ACTGGAATTA CCCTCGAGGG 
TACCCCTATA ATGGCTCTAA CTATGTGAAC GTCACAATGC CGTTGAGGTA TCCTCACACC 
AGGGTGGCGT ACATTTTCTT TGGACCAGGT GTGGACGTGC AGAGCTTCAG CCTGCGTAGC 
GATGATCAGA GGGTGGATAT TTACCTGTTC ACCAAGGACC ACACTTACAC CATCTACCTG 
CTGACCGGAG AGGTCACCAG CAAGCCGCTG TTCTCCATGG TGCTACTTGA CCACAAGAAA 
ATTGTATTCG AGAAGTCAGC GGCTGTGGTA GACAGCTCGC CTGAGGAAGT GGAGGAATAC 
GTCAACGTGA TGGAGGACAA CCTCCAGCAT GTCAAACCCG TCTTCCAGCA GATGGAGAGG 
CACATCCTGG GACGGGTTCT CAACACTGCC AGCTTCCGTA AGACTGCAGA GCGCCTCCTC 
CAGTTCTCCG ACAAGAAGAA GACGGAGGAG GTCATTGAGA AGATGTTCGC CATGTCCAAG 
AAACAGAGCA AAGGCAAAGG GGGGAAGAAA GTGAACCTCG GGGAAAAGCT TTCTGAGAAT 
CTGCCTGACA TTTTTGCACA GATTGAGGTG AATGAGAAGA AGGAGAGACA GAGGACTTCA 
AAGAGAACTT ATGAGGACAG TCCGGAGGAG GGGGACGCAG ACTCACGGGC CTTTATGGAT 
TATACAGATG GCCGCAAGAA TAGAAAGGGC GGCTTTGTCA AGGGCCGCAA ATTCAAAGAG 
GTTCACATGG TGGCCACAGC AGGGAGCGAG AGTCTCCCCA GCACAGCTTC CTACATTAGA 
CTGTTCCTCC TCCTGAACAC TGCCACCTTC TTTTTACTGC TAGCTGTGCT ACTGACTCGC 
TTTCAGAGGG GTCGGAGTCT GCACACTCAG AGATGTTTCT ACACCATCCT CCTGATCGAC 
TGCTTCATCC TACTGTACCT CTACTCCTCC TGCTCTCACA GTCAGTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023152741.1
CDS	214..3084
Translation	MRTHTRGAPTVFFISLLWPLLGPAATADVDPGGGIPFMGGNYDGHPMLYFGRGDVEELQYAAAGTHRDMARRIREAGEAMLEHPEEYLPPWSPAEFSARWNEVYGNNLGVLSMFCLLYPHRAGALDLAKDYMERMAAQPSWLVKDAPWDEVPLAHSLVGFATAYDFLYEYLNKGQQERFLQVIGNASRLMYEKSYVRGWGFQYLHNHQPTNCVALLTGSLVYMTQGYLQEAYLWTKQVLSIMEKFMVLLQDVTDGSLYEGVAYGTYTTRSLFQYMFLVQRHFAISHFDHPWLLKHFAFLYRTILPGFQRTVGIADSNYNWFYGPESQLVFLDRYVLRNGSGNWLADLIHQNRVIEGPGQPGKGQRWCTLHTEFIWYDPGLIPKPPSDFGTSQLHYFEDWGVVTYGSTLPANTNCTFLSFKSGKLGGRAIFDIVHRNKYKEWIKGWRNFNAGHEHPDQNTFTFAPNGVPFITEGLYGPKYTLLNNAVLFGSAFSGSCFKPWAGQVTEACDSKWLKYKVGLAADAQGRVEAAMEREGMVFIRGEGHSAYNPELKIRNFQRNLLLLHPQLLLLVDHIHLDPDSPVRAMSAFFHNTELPFQSTKIDGVHGAFVSHKEDKYKMFWMDDTSYSNKGVLGYWNYPRGYPYNGSNYVNVTMPLRYPHTRVAYIFFGPGVDVQSFSLRSDDQRVDIYLFTKDHTYTIYLLTGEVTSKPLFSMVLLDHKKIVFEKSAAVVDSSPEEVEEYVNVMEDNLQHVKPVFQQMERHILGRVLNTASFRKTAERLLQFSDKKKTEEVIEKMFAMSKKQSKGKGGKKVNLGEKLSENLPDIFAQIEVNEKKERQRTSKRTYEDSPEEGDADSRAFMDYTDGRKNRKGGFVKGRKFKEVHMVATAGSESLPSTASYIRLFLLLNTATFFLLLAVLLTRFQRGRSLHTQRCFYTILLIDCFILLYLYSSCSHSQC

Target ORF information:

RefSeq Version	XM_023296973.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris dermatan sulfate epimerase (dse), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023296973.1

ORF Insert Sequence:

ATGAGAACCC ACACCCGCGG GGCGCCCACA GTCTTCTTCA TAAGTCTGCT GTGGCCCCTG 
CTTGGTCCGG CGGCCACGGC AGATGTGGAC CCCGGCGGAG GAATCCCCTT CATGGGAGGC 
AACTACGATG GTCACCCGAT GCTGTACTTT GGTCGCGGCG ACGTGGAGGA GCTGCAGTAC 
GCCGCGGCAG GGACTCACCG CGATATGGCG AGGAGGATCC GCGAGGCTGG GGAAGCCATG 
CTGGAGCACC CAGAGGAGTA CCTGCCCCCC TGGAGCCCCG CTGAGTTCAG TGCCCGCTGG 
AACGAGGTTT ATGGGAACAA CCTGGGAGTG CTGTCCATGT TCTGTTTGCT GTACCCGCAC 
CGGGCCGGAG CCCTTGACCT GGCCAAAGAC TACATGGAGA GGATGGCAGC TCAGCCTAGT 
TGGCTGGTCA AAGACGCCCC CTGGGATGAA GTTCCTCTGG CGCACTCTTT GGTCGGGTTT 
GCCACCGCTT ATGACTTCCT GTACGAGTAC TTGAACAAGG GCCAGCAGGA GCGTTTCCTG 
CAGGTGATCG GCAACGCGTC ACGCCTGATG TACGAGAAGT CCTACGTCCG GGGTTGGGGG 
TTCCAGTACC TGCACAACCA CCAGCCCACC AACTGTGTGG CTCTGCTCAC AGGAAGTCTG 
GTTTACATGA CCCAAGGTTA CCTTCAGGAG GCCTACCTGT GGACCAAGCA GGTCCTGTCC 
ATCATGGAGA AGTTCATGGT CCTTCTGCAG GATGTGACAG ACGGCTCCCT GTATGAAGGC 
GTGGCCTACG GGACATACAC CACCCGTTCT CTGTTTCAGT ACATGTTTCT GGTGCAGCGC 
CACTTTGCCA TCAGCCACTT CGACCACCCC TGGCTCCTAA AACACTTTGC TTTCCTGTAC 
AGGACCATCC TGCCTGGATT TCAGCGAACT GTAGGTATCG CAGATTCCAA CTACAACTGG 
TTCTACGGAC CAGAGAGCCA GCTGGTCTTC TTGGACCGTT ACGTGTTGCG TAATGGCAGT 
GGAAACTGGC TGGCAGACCT GATTCATCAA AACAGAGTGA TTGAAGGGCC AGGGCAGCCT 
GGTAAAGGCC AGCGATGGTG TACTCTACAC ACAGAGTTCA TCTGGTATGA TCCAGGCCTG 
ATCCCCAAAC CCCCGTCAGA TTTTGGAACG TCGCAGCTCC ACTACTTCGA GGACTGGGGC 
GTGGTCACAT ATGGCAGCAC TCTACCTGCC AACACCAACT GTACCTTTCT GTCCTTCAAG 
TCGGGGAAGC TCGGGGGACG CGCTATCTTC GACATCGTTC ACAGGAACAA GTACAAGGAG 
TGGATCAAAG GCTGGAGGAA CTTTAACGCT GGCCACGAGC ACCCCGATCA GAACACGTTC 
ACCTTTGCAC CCAACGGCGT CCCGTTCATT ACTGAAGGAC TGTACGGACC CAAGTACACT 
TTGTTGAACA ATGCGGTGTT GTTTGGCTCG GCGTTCTCAG GGAGCTGCTT TAAGCCGTGG 
GCGGGACAAG TTACAGAAGC CTGCGACTCC AAGTGGTTGA AATACAAGGT GGGGCTTGCA 
GCTGATGCTC AGGGCAGAGT GGAAGCTGCT ATGGAGAGAG AGGGAATGGT CTTCATCCGA 
GGCGAGGGAC ATTCCGCTTA TAATCCAGAA CTGAAAATCA GGAACTTCCA GAGGAACCTG 
CTCCTTCTTC ACCCGCAGCT CCTGCTCCTC GTGGACCACA TCCACCTGGA TCCAGACAGC 
CCAGTCAGGG CCATGAGTGC CTTCTTTCAC AACACGGAGC TGCCGTTCCA AAGCACAAAG 
ATAGATGGTG TTCATGGAGC GTTTGTGTCA CATAAAGAGG ATAAATATAA GATGTTCTGG 
ATGGATGACA CCAGCTACAG TAACAAAGGG GTGTTGGGTT ACTGGAATTA CCCTCGAGGG 
TACCCCTATA ATGGCTCTAA CTATGTGAAC GTCACAATGC CGTTGAGGTA TCCTCACACC 
AGGGTGGCGT ACATTTTCTT TGGACCAGGT GTGGACGTGC AGAGCTTCAG CCTGCGTAGC 
GATGATCAGA GGGTGGATAT TTACCTGTTC ACCAAGGACC ACACTTACAC CATCTACCTG 
CTGACCGGAG AGGTCACCAG CAAGCCGCTG TTCTCCATGG TGCTACTTGA CCACAAGAAA 
ATTGTATTCG AGAAGTCAGC GGCTGTGGTA GACAGCTCGC CTGAGGAAGT GGAGGAATAC 
GTCAACGTGA TGGAGGACAA CCTCCAGCAT GTCAAACCCG TCTTCCAGCA GATGGAGAGG 
CACATCCTGG GACGGGTTCT CAACACTGCC AGCTTCCGTA AGACTGCAGA GCGCCTCCTC 
CAGTTCTCCG ACAAGAAGAA GACGGAGGAG GTCATTGAGA AGATGTTCGC CATGTCCAAG 
AAACAGAGCA AAGGCAAAGG GGGGAAGAAA GTGAACCTCG GGGAAAAGCT TTCTGAGAAT 
CTGCCTGACA TTTTTGCACA GATTGAGGTG AATGAGAAGA AGGAGAGACA GAGGACTTCA 
AAGAGAACTT ATGAGGACAG TCCGGAGGAG GGGGACGCAG ACTCACGGGC CTTTATGGAT 
TATACAGATG GCCGCAAGAA TAGAAAGGGC GGCTTTGTCA AGGGCCGCAA ATTCAAAGAG 
GTTCACATGG TGGCCACAGC AGGGAGCGAG AGTCTCCCCA GCACAGCTTC CTACATTAGA 
CTGTTCCTCC TCCTGAACAC TGCCACCTTC TTTTTACTGC TAGCTGTGCT ACTGACTCGC 
TTTCAGAGGG GTCGGAGTCT GCACACTCAG AGATGTTTCT ACACCATCCT CCTGATCGAC 
TGCTTCATCC TACTGTACCT CTACTCCTCC TGCTCTCACA GTCAGTGCTA A