mgmt cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mgmt
Entrez Gene ID	111566889
Full Name	O-6-methylguanine-DNA methyltransferase
Gene Type	protein-coding
Organism	Amphiprion ocellaris(clown anemonefish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023267696.1	XP_023123464.1	methylated-DNA--protein-cysteine methyltransferase

Pathways from BioSystems
Gene Ontology

The following mgmt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mgmt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm75860	XM_023267696.1 Latest version!	Amphiprion ocellaris O-6-methylguanine-DNA methyltransferase (mgmt), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mgmt ( XM_023267696.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023123464.1 Amphiprion ocellaris O-6-methylguanine-DNA methyltransferase (mgmt), mRNA.

Price & Availability↑

CloneID	OAm75860
Clone ID Related Accession (Same CDS sequence)	XM_023267696.1
Accession Version	XM_023267696.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	methylated-DNA--protein-cysteine methyltransferase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019371858.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGGG CTTTATTTGC AAGCTGCAGG GCTCATTTAC TGTGCTTAAC GTCCTCTAAG 
AAAGACTACA GTTTAAAAAA TTACCGTGTC CTTAAACCAC CGAGCTGTAT ATTGTGGCAG 
GGCAGCAAGA TGAAGAAGCA GAGGGAGAGC CAGTGCACTC AGAAGACCAT CTCATTGCTG 
AGCCCCCTGG GGACGATCCA AGTCAGTGGA TGTGAGAATG GTGTGCACAC CATCCAGATC 
CTAATGGATG TCGCACCTGC TGAAAGGAGC AGCGAGGCCC CACTGAGCCG TGTGGTCAAC 
GATAGCCCGG CAGAAACGAG CCCCGAGTTG CAGCGCTGCG TTGAATGGCT CCAGGCGTAC 
TTCAGTGAGC CGCAGACCGC TGGGAGTCTC CCGCTCCCTG CCTTTCACCA CCCCGCCCTG 
CAAGGAGATG CGTTCTCCAG CCGTGTGTTG CAGATACTTC AGAGGGATGT GAAGTTTGGA 
GAGACGGTCT CTTACAAACG CCTCGCTGAG ATGGCGGGAA ACCCCAAAGC GGCGCGTGCG 
GTGGGAGGGG CCATGAGAAT GAACCCGGTT CCTCTCCTCA TTCCTTGTCA CAGAGTCATT 
TCGAGCAGTG GACAGAGTGG GCCGTACATG AGCGGCAAGG GCGACCATCT CAAACAATGG 
CTGCTCACCC ATGAGAGGCA GAGAGGGAAA GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023123464.1
CDS	85..780
Translation	MQRALFASCRAHLLCLTSSKKDYSLKNYRVLKPPSCILWQGSKMKKQRESQCTQKTISLLSPLGTIQVSGCENGVHTIQILMDVAPAERSSEAPLSRVVNDSPAETSPELQRCVEWLQAYFSEPQTAGSLPLPAFHHPALQGDAFSSRVLQILQRDVKFGETVSYKRLAEMAGNPKAARAVGGAMRMNPVPLLIPCHRVISSSGQSGPYMSGKGDHLKQWLLTHERQRGKG

Target ORF information:

RefSeq Version	XM_023267696.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris O-6-methylguanine-DNA methyltransferase (mgmt), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023267696.1

ORF Insert Sequence:

ATGCAGAGGG CTTTATTTGC AAGCTGCAGG GCTCATTTAC TGTGCTTAAC GTCCTCTAAG 
AAAGACTACA GTTTAAAAAA TTACCGTGTC CTTAAACCAC CGAGCTGTAT ATTGTGGCAG 
GGCAGCAAGA TGAAGAAGCA GAGGGAGAGC CAGTGCACTC AGAAGACCAT CTCATTGCTG 
AGCCCCCTGG GGACGATCCA AGTCAGTGGA TGTGAGAATG GTGTGCACAC CATCCAGATC 
CTAATGGATG TCGCACCTGC TGAAAGGAGC AGCGAGGCCC CACTGAGCCG TGTGGTCAAC 
GATAGCCCGG CAGAAACGAG CCCCGAGTTG CAGCGCTGCG TTGAATGGCT CCAGGCGTAC 
TTCAGTGAGC CGCAGACCGC TGGGAGTCTC CCGCTCCCTG CCTTTCACCA CCCCGCCCTG 
CAAGGAGATG CGTTCTCCAG CCGTGTGTTG CAGATACTTC AGAGGGATGT GAAGTTTGGA 
GAGACGGTCT CTTACAAACG CCTCGCTGAG ATGGCGGGAA ACCCCAAAGC GGCGCGTGCG 
GTGGGAGGGG CCATGAGAAT GAACCCGGTT CCTCTCCTCA TTCCTTGTCA CAGAGTCATT 
TCGAGCAGTG GACAGAGTGG GCCGTACATG AGCGGCAAGG GCGACCATCT CAAACAATGG 
CTGCTCACCC ATGAGAGGCA GAGAGGGAAA GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.