edem2 cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene
Clones
gRNAs

Summary

Gene Symbol	edem2
Entrez Gene ID	111562423
Full Name	ER degradation enhancing alpha-mannosidase like protein 2
Gene Type	protein-coding
Organism	Amphiprion ocellaris(clown anemonefish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023260931.1	XP_023116699.1	ER degradation-enhancing alpha-mannosidase-like protein 2

Pathways from BioSystems
Gene Ontology

The following edem2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the edem2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm100975	XM_023260931.1 Latest version!	Amphiprion ocellaris ER degradation enhancing alpha-mannosidase like protein 2 (edem2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

edem2 ( XM_023260931.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023116699.1 Amphiprion ocellaris ER degradation enhancing alpha-mannosidase like protein 2 (edem2), mRNA.

Price & Availability↑

CloneID	OAm100975
Clone ID Related Accession (Same CDS sequence)	XM_023260931.1
Accession Version	XM_023260931.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	ER degradation-enhancing alpha-mannosidase-like protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019375974.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGCTC CCGTGTGCGC GGCGCTGTTG TTGCTGCTGC TGAGCGATGC GGCGACTCAT 
CAGTTCACTG ACCAGGAGAT GGCTGCTGTC AGGCAGCGCA TCAAGTCCAT GTTCTACCAC 
GCCTACAACA GTTACCTCGA CAACGCCTTC CCCTACGACG AGCTCCGCCC ACTCACCTGT 
GATGGACAAG ACACCTGGGG CAGTTTCTCT CTCACTCTGA TTGATGCCCT CGACACTCTG 
CTGGTTTTGG GAAACCGTAC AGAGTTTCAG CGTGTAGCAT CACTTCTCCA AGACACTGTG 
GACTTCGACA TTGACGTCAA TGCATCTGTG TTTGAAACCA ACATTAGAGT GGTGGGTGGT 
CTGCTGTCGG CCCACCTGCT GGCAGGGAGG GCAGGGATGG AGCTGGAGCC TGGTTGGCCT 
TGTTCAGGAC CACTGCTGAG GATGGCCGAG GACGCCGCCA GGAAACTACT GCCTGCATTT 
CAGACTCCTA CTGGGATGCC GTACGGTACG GTGAACCTGC TGCACGGCGT CAGTCCCACC 
GAGACACCTG TCACCTGCAC TGCCGGCGTG GGAACCTTCA TCCTGGAGTT TGCTATGCTG 
AGTCGATTAA CTGGAGACCC GACGTTTGAG AATGCAGCCC GCCGAGCTCT GAGAGCCCTA 
TGGAAGACCA GATCTGACAT CGGACTGGTG GGGAACCACA TTGATATCCT GTCTAAAAAG 
TGGGTGGCTC AAGATGCTGG GATCGGAGCG GGTGTCGATT CTTACTTTGA GTATCTGGTG 
AAAGGAGCCA TCATGCTGCA AGACGAAGAG CTGCTTAACA TGTTCCAGGC TTACGATCGA 
GCCATTCAGA ACTACACTCA TTTTGACGAT TGGTACCTGT GGGTGCAAAT GCATAAAGGA 
ACAGTGTCCA TGCCTGTTTT TCAAAGTCTG GAGGCCTTCT GGCCCGGCCT GCAGTCTCTC 
CTGGGAAACC TGGACAGTGC CGTCAGAACA TTTCAGAACT ATTATTCGGT GTGGAGACAG 
TTTGGAGGTC TGCCGGAGTT TTATAGCATC CCCCAGGGTT ACACTGTGGA TAAGAGAGAG 
GGATACCCGC TGAGGCCAGA GTTAGTAGAG AGTGCCATGT ACCTGTTCAG GGCCACAGGC 
GACCACACCT ACCTCCAGCT GGGCTTCGAC GCTCTGGAGT CCATCGAGAG GATCGCCAAG 
ACGCCCTGTG GATATGCCAG CGTTAAAGAC CTGCGAGATC ACCAGCTGGA CAACAGGATG 
GAGTCCTTTT TCCTCGCTGA AACCATAAAA TACCTCTACC TGCTCTTTGA CCCCACCCAC 
TTCCTGCATG GCGGAGGGAC GGAGGGCGAC GGTTCCTGGG AGGAAGGGGG TGATGGGGGG 
CAGTGTGTTT TAGGGGCTGG GGGGTTCATC TTCAACACAG AAGCTCATCC TCTGGACCCA 
GCGGCGCTCT ACTGCTGCAG CCGCCACGCC CAGGACCATC AGGAGCTCCG AGACATCCTG 
CTCAGCCTGT CACAGCCCGA CGGTCGATCA AAACCGCCTG CCAAGCAAAC AGAAGACCCT 
CCACCCAACC CGTCAGACAG CATTGCTCTG AAGCCAGGAG AAAAGAGGAG GCCTCCACTG 
CTGTCCTGCC CCGTTCAGCC ATTCAGCGCT CGGCTGTCCA TCTTTGGACA GGTGTTCACC 
GACAACACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023116699.1
CDS	138..1829
Translation	MRAPVCAALLLLLLSDAATHQFTDQEMAAVRQRIKSMFYHAYNSYLDNAFPYDELRPLTCDGQDTWGSFSLTLIDALDTLLVLGNRTEFQRVASLLQDTVDFDIDVNASVFETNIRVVGGLLSAHLLAGRAGMELEPGWPCSGPLLRMAEDAARKLLPAFQTPTGMPYGTVNLLHGVSPTETPVTCTAGVGTFILEFAMLSRLTGDPTFENAARRALRALWKTRSDIGLVGNHIDILSKKWVAQDAGIGAGVDSYFEYLVKGAIMLQDEELLNMFQAYDRAIQNYTHFDDWYLWVQMHKGTVSMPVFQSLEAFWPGLQSLLGNLDSAVRTFQNYYSVWRQFGGLPEFYSIPQGYTVDKREGYPLRPELVESAMYLFRATGDHTYLQLGFDALESIERIAKTPCGYASVKDLRDHQLDNRMESFFLAETIKYLYLLFDPTHFLHGGGTEGDGSWEEGGDGGQCVLGAGGFIFNTEAHPLDPAALYCCSRHAQDHQELRDILLSLSQPDGRSKPPAKQTEDPPPNPSDSIALKPGEKRRPPLLSCPVQPFSARLSIFGQVFTDNT

Target ORF information:

RefSeq Version	XM_023260931.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris ER degradation enhancing alpha-mannosidase like protein 2 (edem2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023260931.1

ORF Insert Sequence:

ATGCGCGCTC CCGTGTGCGC GGCGCTGTTG TTGCTGCTGC TGAGCGATGC GGCGACTCAT 
CAGTTCACTG ACCAGGAGAT GGCTGCTGTC AGGCAGCGCA TCAAGTCCAT GTTCTACCAC 
GCCTACAACA GTTACCTCGA CAACGCCTTC CCCTACGACG AGCTCCGCCC ACTCACCTGT 
GATGGACAAG ACACCTGGGG CAGTTTCTCT CTCACTCTGA TTGATGCCCT CGACACTCTG 
CTGGTTTTGG GAAACCGTAC AGAGTTTCAG CGTGTAGCAT CACTTCTCCA AGACACTGTG 
GACTTCGACA TTGACGTCAA TGCATCTGTG TTTGAAACCA ACATTAGAGT GGTGGGTGGT 
CTGCTGTCGG CCCACCTGCT GGCAGGGAGG GCAGGGATGG AGCTGGAGCC TGGTTGGCCT 
TGTTCAGGAC CACTGCTGAG GATGGCCGAG GACGCCGCCA GGAAACTACT GCCTGCATTT 
CAGACTCCTA CTGGGATGCC GTACGGTACG GTGAACCTGC TGCACGGCGT CAGTCCCACC 
GAGACACCTG TCACCTGCAC TGCCGGCGTG GGAACCTTCA TCCTGGAGTT TGCTATGCTG 
AGTCGATTAA CTGGAGACCC GACGTTTGAG AATGCAGCCC GCCGAGCTCT GAGAGCCCTA 
TGGAAGACCA GATCTGACAT CGGACTGGTG GGGAACCACA TTGATATCCT GTCTAAAAAG 
TGGGTGGCTC AAGATGCTGG GATCGGAGCG GGTGTCGATT CTTACTTTGA GTATCTGGTG 
AAAGGAGCCA TCATGCTGCA AGACGAAGAG CTGCTTAACA TGTTCCAGGC TTACGATCGA 
GCCATTCAGA ACTACACTCA TTTTGACGAT TGGTACCTGT GGGTGCAAAT GCATAAAGGA 
ACAGTGTCCA TGCCTGTTTT TCAAAGTCTG GAGGCCTTCT GGCCCGGCCT GCAGTCTCTC 
CTGGGAAACC TGGACAGTGC CGTCAGAACA TTTCAGAACT ATTATTCGGT GTGGAGACAG 
TTTGGAGGTC TGCCGGAGTT TTATAGCATC CCCCAGGGTT ACACTGTGGA TAAGAGAGAG 
GGATACCCGC TGAGGCCAGA GTTAGTAGAG AGTGCCATGT ACCTGTTCAG GGCCACAGGC 
GACCACACCT ACCTCCAGCT GGGCTTCGAC GCTCTGGAGT CCATCGAGAG GATCGCCAAG 
ACGCCCTGTG GATATGCCAG CGTTAAAGAC CTGCGAGATC ACCAGCTGGA CAACAGGATG 
GAGTCCTTTT TCCTCGCTGA AACCATAAAA TACCTCTACC TGCTCTTTGA CCCCACCCAC 
TTCCTGCATG GCGGAGGGAC GGAGGGCGAC GGTTCCTGGG AGGAAGGGGG TGATGGGGGG 
CAGTGTGTTT TAGGGGCTGG GGGGTTCATC TTCAACACAG AAGCTCATCC TCTGGACCCA 
GCGGCGCTCT ACTGCTGCAG CCGCCACGCC CAGGACCATC AGGAGCTCCG AGACATCCTG 
CTCAGCCTGT CACAGCCCGA CGGTCGATCA AAACCGCCTG CCAAGCAAAC AGAAGACCCT 
CCACCCAACC CGTCAGACAG CATTGCTCTG AAGCCAGGAG AAAAGAGGAG GCCTCCACTG 
CTGTCCTGCC CCGTTCAGCC ATTCAGCGCT CGGCTGTCCA TCTTTGGACA GGTGTTCACC 
GACAACACCT GA