SI:CH1073-179P4.3 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SI:CH1073-179P4.3
Entrez Gene ID	798259
Full Name	thioredoxin reductase 2, tandem duplicate 2
Synonyms	im:7135991,si:ch1073-179p4.3,txnrd2
General protein information	Preferred Names thioredoxin reductase 2, tandem duplicate 2 Names thioredoxin reductase 2, mitochondrial thioredoxin reductase 2
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)
Summary	The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes a mitochondrial form important for scavenging reactive oxygen species in mitochondria. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2017].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017356234.1	XP_017211723.1	thioredoxin reductase 2, mitochondrial isoform X3
NM_001353396.1	NP_001340325.1	thioredoxin reductase 2, mitochondrial isoform 1
XM_009301254.2	XP_009299529.1	thioredoxin reductase 2, mitochondrial isoform X1
NM_001353394.1	NP_001340323.1	thioredoxin reductase 2, mitochondrial isoform 2
XM_009301255.2	XP_009299530.1	thioredoxin reductase 2, mitochondrial isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Selenoprotein Gene Nomenclature.
Gladyshev VN, Arn?r ES, Berry MJ, Brigelius-Floh? R, Bruford EA, Burk RF, Carlson BA, Castellano S, Chavatte L, Conrad M, Copeland PR, Diamond AM, Driscoll DM, Ferreiro A, Floh? L, Green FR, Guig? R, Handy DE, Hatfield DL, Hesketh J, Hoffmann PR, Holmgren A, Hondal RJ, Howard MT, Huang K, Kim HY, Kim IY, K?hrle J, Krol A, Kryukov GV, Lee BJ, Lee BC, Lei XG, Liu Q, Lescure A, Lobanov AV, Loscalzo J, Maiorino M, Mariotti M, Sandeep Prabhu K, Rayman MP, Rozovsky S, Salinas G, Schmidt EE, Schomburg L, Schweizer U, Simonovi? M, Sunde RA, Tsuji PA, Tweedie S, Ursini F, Whanger PD, Zhang Y
The Journal of biological chemistry291(46)24036-24040(2016 Nov)

Comparative mapping of the 22q11.2 deletion region and the potential of simple model organisms.
Guna A, Butcher NJ, Bassett AS
Journal of neurodevelopmental disorders7(1)18(2015)

Selenium prevents downregulation of antioxidant selenoprotein genes by methylmercury.
Penglase S, Hamre K, Ellingsen S
Free radical biology & medicine7595-104(2014 Oct)

Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SI:CH1073-179P4.3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SI:CH1073-179P4.3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa47255	XM_009301254.2 Latest version!	Danio rerio si:ch1073-179p4.3 (si:ch1073-179p4.3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa47256	XM_009301255.2 Latest version!	Danio rerio si:ch1073-179p4.3 (si:ch1073-179p4.3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa59192	XM_017356234.1 Latest version!	Danio rerio si:ch1073-179p4.3 (si:ch1073-179p4.3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
ODa141160	NM_001353396.1 Latest version!	Danio rerio thioredoxin reductase 2, tandem duplicate 2 (txnrd2.2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa141161	NM_001353394.1 Latest version!	Danio rerio thioredoxin reductase 2, tandem duplicate 2 (txnrd2.2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SI:CH1073-179P4.3 ( XM_009301254.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009299529.1 Danio rerio si:ch1073-179p4.3 (si:ch1073-179p4.3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa47255
Clone ID Related Accession (Same CDS sequence)	XM_009301254.2
Accession Version	XM_009301254.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	thioredoxin reductase 2, mitochondrial isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301254.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCT TCAGTAGAGG CAGGCAGAGG GTGAAAACTC TCTGCTGTTT CAGGACTTTA 
ATACTTTCAA GAAACTTTAC AGCAGGTAAA TTTGATTATG ATTTGGTGGT TATTGGTGGA 
GGCTCTGGAG GGTTGGCATG TTCAAAAGAA GCTGCCCAGT TAGGACAGAA AGTTGCTGTC 
TTGGACTATG TTGAACCTTC ACTCAAAGGC ACAAAGTGGG GTCTCGGTGG CACGTGTGTG 
AATGTCGGCT GTATTCCAAA GAAGCTTATG CATCAGGCAG CACTGCTTGG CACTGCTGTC 
AAAGATGCTA GGAAGTATGG CTGGCAAATC CCAGAAACCC TGTCACATGA CTGGCCAACA 
ATGGCTGAGG CTGTCCAGAA CCATGTTAGG TCTCTAAACT GGGGTCACAG AGTTCAACTA 
CAAGACAAGA AGGTGAAATA TTTAAATATG AAGGGCACTC TGCTGGATAA ACACACTGTC 
AGAGCTGTAA ATGCCCAAGG GAAGGAGATG ACAGTGACCG CCAGAAACAT TCTGTTAGCT 
ACTGGTGGAC GGCCAAAGTA CCCTACACAT GTCCCAGGTG CCATGGAGTT TGGGATCACA 
AGTGATGATA TTTTCTGGCT CAAAGAGTCT CCCAAAAAAA CGTTGATCAT TGGTGCAAGC 
TATGTATCGC TGGAGTGTGC TGGTTTTCTT ACAGGCATTG GGCTGGACAC CAGTGTAATG 
GTGCGCAGTA TCGCCCTCCG CGGGTTTGAT CAGCAAATGT CTGGTTTAGT CACAGACTAC 
ATGGAGACGT ATGGCACGAA GTTCCACTGG AAGTGCACAC CGAAAAGTGT AGAAAAGCTG 
CCATCTGGCC ATCTTCAAGT CACATGGATG GATTTAAACA CCAAAGAGGA ACATCAAGAC 
ACATTCAACT CAGTACTTTG GGCTGTAGGC AGAGCCCCTG AAACCAAGAC TCTTAATTTG 
GAAAAGGTTG GCGTGAAGAT CAACAAAGAA ACAGGAAAGA TAATCGTGGC TGCTGACGAA 
GCCACGTCTG TGCCGAATAT TTTTGCTATT GGAGACATCG GTGAGGGTCG TCCTGAGTTG 
ACCCCCACCG CCATTAAGGC TGGAAAACTC CTGGCCCACA GACTGGCTGG TCGTTCCACT 
GAGCTCATGA ATTACGAGAA TGTAGCTACT ACAGTGTTCA CACCACTAGA ATATGGCTGT 
GTAGGTCTGT CTGAAGAAGA GGCAGAAAGG AGACATGGAA AAGACCAGAT TGAGGTGTAC 
CATGCTTTCT ACAAACCGCT GGAGTTCACT GTGGCTGAGA GAGATGCCAC TCAATGCTAT 
ATAAAGGTGG TTTGTCTACG AGAAGGTGAC CAGCGCGTGC TGGGTCTGCA TTTTACTGGG 
CCGAACGCTG GTGAAGTCAC ACAGGGCTTT GCTTTGGGCT TCCAGTGTGG ACTTACATAC 
GAACACCTCA GGAACACAGT GGGGATTCAC CCCACCTGTG CTGAGGAGCT CACCAAACTC 
AACATCACCA AACGCTCGGG TCTTGACGCC ACGGTGACAG GCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299529.1
CDS	253..1800
Translation	MAAFSRGRQRVKTLCCFRTLILSRNFTAGKFDYDLVVIGGGSGGLACSKEAAQLGQKVAVLDYVEPSLKGTKWGLGGTCVNVGCIPKKLMHQAALLGTAVKDARKYGWQIPETLSHDWPTMAEAVQNHVRSLNWGHRVQLQDKKVKYLNMKGTLLDKHTVRAVNAQGKEMTVTARNILLATGGRPKYPTHVPGAMEFGITSDDIFWLKESPKKTLIIGASYVSLECAGFLTGIGLDTSVMVRSIALRGFDQQMSGLVTDYMETYGTKFHWKCTPKSVEKLPSGHLQVTWMDLNTKEEHQDTFNSVLWAVGRAPETKTLNLEKVGVKINKETGKIIVAADEATSVPNIFAIGDIGEGRPELTPTAIKAGKLLAHRLAGRSTELMNYENVATTVFTPLEYGCVGLSEEEAERRHGKDQIEVYHAFYKPLEFTVAERDATQCYIKVVCLREGDQRVLGLHFTGPNAGEVTQGFALGFQCGLTYEHLRNTVGIHPTCAEELTKLNITKRSGLDATVTGC

Target ORF information:

RefSeq Version	XM_009301254.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch1073-179p4.3 (si:ch1073-179p4.3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301254.2

ORF Insert Sequence:

ATGGCTGCCT TCAGTAGAGG CAGGCAGAGG GTGAAAACTC TCTGCTGTTT CAGGACTTTA 
ATACTTTCAA GAAACTTTAC AGCAGGTAAA TTTGATTATG ATTTGGTGGT TATTGGTGGA 
GGCTCTGGAG GGTTGGCATG TTCAAAAGAA GCTGCCCAGT TAGGACAGAA AGTTGCTGTC 
TTGGACTATG TTGAACCTTC ACTCAAAGGC ACAAAGTGGG GTCTCGGTGG CACGTGTGTG 
AATGTCGGCT GTATTCCAAA GAAGCTTATG CATCAGGCAG CACTGCTTGG CACTGCTGTC 
AAAGATGCTA GGAAGTATGG CTGGCAAATC CCAGAAACCC TGTCACATGA CTGGCCAACA 
ATGGCTGAGG CTGTCCAGAA CCATGTTAGG TCTCTAAACT GGGGTCACAG AGTTCAACTA 
CAAGACAAGA AGGTGAAATA TTTAAATATG AAGGGCACTC TGCTGGATAA ACACACTGTC 
AGAGCTGTAA ATGCCCAAGG GAAGGAGATG ACAGTGACCG CCAGAAACAT TCTGTTAGCT 
ACTGGTGGAC GGCCAAAGTA CCCTACACAT GTCCCAGGTG CCATGGAGTT TGGGATCACA 
AGTGATGATA TTTTCTGGCT CAAAGAGTCT CCCAAAAAAA CGTTGATCAT TGGTGCAAGC 
TATGTATCGC TGGAGTGTGC TGGTTTTCTT ACAGGCATTG GGCTGGACAC CAGTGTAATG 
GTGCGCAGTA TCGCCCTCCG CGGGTTTGAT CAGCAAATGT CTGGTTTAGT CACAGACTAC 
ATGGAGACGT ATGGCACGAA GTTCCACTGG AAGTGCACAC CGAAAAGTGT AGAAAAGCTG 
CCATCTGGCC ATCTTCAAGT CACATGGATG GATTTAAACA CCAAAGAGGA ACATCAAGAC 
ACATTCAACT CAGTACTTTG GGCTGTAGGC AGAGCCCCTG AAACCAAGAC TCTTAATTTG 
GAAAAGGTTG GCGTGAAGAT CAACAAAGAA ACAGGAAAGA TAATCGTGGC TGCTGACGAA 
GCCACGTCTG TGCCGAATAT TTTTGCTATT GGAGACATCG GTGAGGGTCG TCCTGAGTTG 
ACCCCCACCG CCATTAAGGC TGGAAAACTC CTGGCCCACA GACTGGCTGG TCGTTCCACT 
GAGCTCATGA ATTACGAGAA TGTAGCTACT ACAGTGTTCA CACCACTAGA ATATGGCTGT 
GTAGGTCTGT CTGAAGAAGA GGCAGAAAGG AGACATGGAA AAGACCAGAT TGAGGTGTAC 
CATGCTTTCT ACAAACCGCT GGAGTTCACT GTGGCTGAGA GAGATGCCAC TCAATGCTAT 
ATAAAGGTGG TTTGTCTACG AGAAGGTGAC CAGCGCGTGC TGGGTCTGCA TTTTACTGGG 
CCGAACGCTG GTGAAGTCAC ACAGGGCTTT GCTTTGGGCT TCCAGTGTGG ACTTACATAC 
GAACACCTCA GGAACACAGT GGGGATTCAC CCCACCTGTG CTGAGGAGCT CACCAAACTC 
AACATCACCA AACGCTCGGG TCTTGACGCC ACGGTGACAG GCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH1073-179P4.3 ( XM_009301255.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009299530.1 Danio rerio si:ch1073-179p4.3 (si:ch1073-179p4.3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa47256
Clone ID Related Accession (Same CDS sequence)	XM_009301255.2
Accession Version	XM_009301255.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	thioredoxin reductase 2, mitochondrial isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301255.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCT TCAGTAGAGG CAGGCAGAGG GTGAAAACTC TCTGCTGTTT CAGGACTTTA 
ATACTTTCAA GAAACTTTAC AGGTAAATTT GATTATGATT TGGTGGTTAT TGGTGGAGGC 
TCTGGAGGGT TGGCATGTTC AAAAGAAGCT GCCCAGTTAG GACAGAAAGT TGCTGTCTTG 
GACTATGTTG AACCTTCACT CAAAGGCACA AAGTGGGGTC TCGGTGGCAC GTGTGTGAAT 
GTCGGCTGTA TTCCAAAGAA GCTTATGCAT CAGGCAGCAC TGCTTGGCAC TGCTGTCAAA 
GATGCTAGGA AGTATGGCTG GCAAATCCCA GAAACCCTGT CACATGACTG GCCAACAATG 
GCTGAGGCTG TCCAGAACCA TGTTAGGTCT CTAAACTGGG GTCACAGAGT TCAACTACAA 
GACAAGAAGG TGAAATATTT AAATATGAAG GGCACTCTGC TGGATAAACA CACTGTCAGA 
GCTGTAAATG CCCAAGGGAA GGAGATGACA GTGACCGCCA GAAACATTCT GTTAGCTACT 
GGTGGACGGC CAAAGTACCC TACACATGTC CCAGGTGCCA TGGAGTTTGG GATCACAAGT 
GATGATATTT TCTGGCTCAA AGAGTCTCCC AAAAAAACGT TGATCATTGG TGCAAGCTAT 
GTATCGCTGG AGTGTGCTGG TTTTCTTACA GGCATTGGGC TGGACACCAG TGTAATGGTG 
CGCAGTATCG CCCTCCGCGG GTTTGATCAG CAAATGTCTG GTTTAGTCAC AGACTACATG 
GAGACGTATG GCACGAAGTT CCACTGGAAG TGCACACCGA AAAGTGTAGA AAAGCTGCCA 
TCTGGCCATC TTCAAGTCAC ATGGATGGAT TTAAACACCA AAGAGGAACA TCAAGACACA 
TTCAACTCAG TACTTTGGGC TGTAGGCAGA GCCCCTGAAA CCAAGACTCT TAATTTGGAA 
AAGGTTGGCG TGAAGATCAA CAAAGAAACA GGAAAGATAA TCGTGGCTGC TGACGAAGCC 
ACGTCTGTGC CGAATATTTT TGCTATTGGA GACATCGGTG AGGGTCGTCC TGAGTTGACC 
CCCACCGCCA TTAAGGCTGG AAAACTCCTG GCCCACAGAC TGGCTGGTCG TTCCACTGAG 
CTCATGAATT ACGAGAATGT AGCTACTACA GTGTTCACAC CACTAGAATA TGGCTGTGTA 
GGTCTGTCTG AAGAAGAGGC AGAAAGGAGA CATGGAAAAG ACCAGATTGA GGTGTACCAT 
GCTTTCTACA AACCGCTGGA GTTCACTGTG GCTGAGAGAG ATGCCACTCA ATGCTATATA 
AAGGTGGTTT GTCTACGAGA AGGTGACCAG CGCGTGCTGG GTCTGCATTT TACTGGGCCG 
AACGCTGGTG AAGTCACACA GGGCTTTGCT TTGGGCTTCC AGTGTGGACT TACATACGAA 
CACCTCAGGA ACACAGTGGG GATTCACCCC ACCTGTGCTG AGGAGCTCAC CAAACTCAAC 
ATCACCAAAC GCTCGGGTCT TGACGCCACG GTGACAGGCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299530.1
CDS	225..1769
Translation	MAAFSRGRQRVKTLCCFRTLILSRNFTGKFDYDLVVIGGGSGGLACSKEAAQLGQKVAVLDYVEPSLKGTKWGLGGTCVNVGCIPKKLMHQAALLGTAVKDARKYGWQIPETLSHDWPTMAEAVQNHVRSLNWGHRVQLQDKKVKYLNMKGTLLDKHTVRAVNAQGKEMTVTARNILLATGGRPKYPTHVPGAMEFGITSDDIFWLKESPKKTLIIGASYVSLECAGFLTGIGLDTSVMVRSIALRGFDQQMSGLVTDYMETYGTKFHWKCTPKSVEKLPSGHLQVTWMDLNTKEEHQDTFNSVLWAVGRAPETKTLNLEKVGVKINKETGKIIVAADEATSVPNIFAIGDIGEGRPELTPTAIKAGKLLAHRLAGRSTELMNYENVATTVFTPLEYGCVGLSEEEAERRHGKDQIEVYHAFYKPLEFTVAERDATQCYIKVVCLREGDQRVLGLHFTGPNAGEVTQGFALGFQCGLTYEHLRNTVGIHPTCAEELTKLNITKRSGLDATVTGC

Target ORF information:

RefSeq Version	XM_009301255.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch1073-179p4.3 (si:ch1073-179p4.3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301255.2

ORF Insert Sequence:

ATGGCTGCCT TCAGTAGAGG CAGGCAGAGG GTGAAAACTC TCTGCTGTTT CAGGACTTTA 
ATACTTTCAA GAAACTTTAC AGGTAAATTT GATTATGATT TGGTGGTTAT TGGTGGAGGC 
TCTGGAGGGT TGGCATGTTC AAAAGAAGCT GCCCAGTTAG GACAGAAAGT TGCTGTCTTG 
GACTATGTTG AACCTTCACT CAAAGGCACA AAGTGGGGTC TCGGTGGCAC GTGTGTGAAT 
GTCGGCTGTA TTCCAAAGAA GCTTATGCAT CAGGCAGCAC TGCTTGGCAC TGCTGTCAAA 
GATGCTAGGA AGTATGGCTG GCAAATCCCA GAAACCCTGT CACATGACTG GCCAACAATG 
GCTGAGGCTG TCCAGAACCA TGTTAGGTCT CTAAACTGGG GTCACAGAGT TCAACTACAA 
GACAAGAAGG TGAAATATTT AAATATGAAG GGCACTCTGC TGGATAAACA CACTGTCAGA 
GCTGTAAATG CCCAAGGGAA GGAGATGACA GTGACCGCCA GAAACATTCT GTTAGCTACT 
GGTGGACGGC CAAAGTACCC TACACATGTC CCAGGTGCCA TGGAGTTTGG GATCACAAGT 
GATGATATTT TCTGGCTCAA AGAGTCTCCC AAAAAAACGT TGATCATTGG TGCAAGCTAT 
GTATCGCTGG AGTGTGCTGG TTTTCTTACA GGCATTGGGC TGGACACCAG TGTAATGGTG 
CGCAGTATCG CCCTCCGCGG GTTTGATCAG CAAATGTCTG GTTTAGTCAC AGACTACATG 
GAGACGTATG GCACGAAGTT CCACTGGAAG TGCACACCGA AAAGTGTAGA AAAGCTGCCA 
TCTGGCCATC TTCAAGTCAC ATGGATGGAT TTAAACACCA AAGAGGAACA TCAAGACACA 
TTCAACTCAG TACTTTGGGC TGTAGGCAGA GCCCCTGAAA CCAAGACTCT TAATTTGGAA 
AAGGTTGGCG TGAAGATCAA CAAAGAAACA GGAAAGATAA TCGTGGCTGC TGACGAAGCC 
ACGTCTGTGC CGAATATTTT TGCTATTGGA GACATCGGTG AGGGTCGTCC TGAGTTGACC 
CCCACCGCCA TTAAGGCTGG AAAACTCCTG GCCCACAGAC TGGCTGGTCG TTCCACTGAG 
CTCATGAATT ACGAGAATGT AGCTACTACA GTGTTCACAC CACTAGAATA TGGCTGTGTA 
GGTCTGTCTG AAGAAGAGGC AGAAAGGAGA CATGGAAAAG ACCAGATTGA GGTGTACCAT 
GCTTTCTACA AACCGCTGGA GTTCACTGTG GCTGAGAGAG ATGCCACTCA ATGCTATATA 
AAGGTGGTTT GTCTACGAGA AGGTGACCAG CGCGTGCTGG GTCTGCATTT TACTGGGCCG 
AACGCTGGTG AAGTCACACA GGGCTTTGCT TTGGGCTTCC AGTGTGGACT TACATACGAA 
CACCTCAGGA ACACAGTGGG GATTCACCCC ACCTGTGCTG AGGAGCTCAC CAAACTCAAC 
ATCACCAAAC GCTCGGGTCT TGACGCCACG GTGACAGGCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH1073-179P4.3 ( XM_017356234.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017211723.1 Danio rerio si:ch1073-179p4.3 (si:ch1073-179p4.3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa59192
Clone ID Related Accession (Same CDS sequence)	XM_017356234.1
Accession Version	XM_017356234.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	thioredoxin reductase 2, mitochondrial isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCT TCAGTAGAGG CAGGCAGAGG GTGAAAACTC TCTGCTGTTT CAGGACTTTA 
ATACTTTCAA GAAACTTTAC AGCAGGTAAA TTTGATTATG ATTTGGTGGT TATTGGTGGA 
GGCTCTGGAG GGTTGGCATG TTCAAAAGAA GCTGCCCAGT TAGGACAGAA AGTTGCTGTC 
TTGGACTATG TTGAACCTTC ACTCAAAGGC ACAAAGTGGG GTCTCGGTGG CACGTGTGTG 
AATGTCGGCT GTATTCCAAA GAAGCTTATG CATCAGGCAG CACTGCTTGG CACTGCTGTC 
AAAGATGCTA GGAAGTATGG CTGGCAAATC CCAGAAACCC TGTCACATGA CTGGCCAACA 
ATGGCTGAGG CTGTCCAGAA CCATGTTAGG TCTCTAAACT GGGGTCACAG AGTTCAACTA 
CAAGACAAGA AGGTGAAATA TTTAAATATG AAGGGCACTC TGCTGGATAA ACACACTGTC 
AGAGCTGTAA ATGCCCAAGG GAAGGAGATG ACAGTGACCG CCAGAAACAT TCTGTTAGCT 
ACTGGTGGAC GGCCAAAGTA CCCTACACAT GTCCCAGGTG CCATGGAGTT TGGGATCACA 
AGTGATGATA TTTTCTGGCT CAAAGAGTCT CCCAAAAAAA CGTTGATCAT TGGTGCAAGC 
TATGTATCGC TGGAGTGTGC TGGTTTTCTT ACAGGCATTG GGCTGGACAC CAGTGTAATG 
GTGCGCAGTA TCGCCCTCCG CGGGTTTGAT CAGCAAATGT CTGGTTTAGT CACAGACTAC 
ATGGAGACGT ATGGCACGAA GTTCCACTGG AAGTGCACAC CGAAAAGTGT AGAAAAGCTG 
CCATCTGGCC ATCTTCAAGT CACATGGATG GATTTAAACA CCAAAGAGGA ACATCAAGAC 
ACATTCAACT CAGTACTTTG GGCTGTAGGT AAGGTCAGCA ATTCTAGAAT GCACACAAAA 
CATTTAATTT CATTGAATAA AAAAAAAACA TCAATGTCAA TCAAAGGTGG CAACACCAAG 
GGTCCAAAAA TACAAATTTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211723.1
CDS	253..1296
Translation	MAAFSRGRQRVKTLCCFRTLILSRNFTAGKFDYDLVVIGGGSGGLACSKEAAQLGQKVAVLDYVEPSLKGTKWGLGGTCVNVGCIPKKLMHQAALLGTAVKDARKYGWQIPETLSHDWPTMAEAVQNHVRSLNWGHRVQLQDKKVKYLNMKGTLLDKHTVRAVNAQGKEMTVTARNILLATGGRPKYPTHVPGAMEFGITSDDIFWLKESPKKTLIIGASYVSLECAGFLTGIGLDTSVMVRSIALRGFDQQMSGLVTDYMETYGTKFHWKCTPKSVEKLPSGHLQVTWMDLNTKEEHQDTFNSVLWAVGKVSNSRMHTKHLISLNKKKTSMSIKGGNTKGPKIQIY

Target ORF information:

RefSeq Version	XM_017356234.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch1073-179p4.3 (si:ch1073-179p4.3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356234.1

ORF Insert Sequence:

ATGGCTGCCT TCAGTAGAGG CAGGCAGAGG GTGAAAACTC TCTGCTGTTT CAGGACTTTA 
ATACTTTCAA GAAACTTTAC AGCAGGTAAA TTTGATTATG ATTTGGTGGT TATTGGTGGA 
GGCTCTGGAG GGTTGGCATG TTCAAAAGAA GCTGCCCAGT TAGGACAGAA AGTTGCTGTC 
TTGGACTATG TTGAACCTTC ACTCAAAGGC ACAAAGTGGG GTCTCGGTGG CACGTGTGTG 
AATGTCGGCT GTATTCCAAA GAAGCTTATG CATCAGGCAG CACTGCTTGG CACTGCTGTC 
AAAGATGCTA GGAAGTATGG CTGGCAAATC CCAGAAACCC TGTCACATGA CTGGCCAACA 
ATGGCTGAGG CTGTCCAGAA CCATGTTAGG TCTCTAAACT GGGGTCACAG AGTTCAACTA 
CAAGACAAGA AGGTGAAATA TTTAAATATG AAGGGCACTC TGCTGGATAA ACACACTGTC 
AGAGCTGTAA ATGCCCAAGG GAAGGAGATG ACAGTGACCG CCAGAAACAT TCTGTTAGCT 
ACTGGTGGAC GGCCAAAGTA CCCTACACAT GTCCCAGGTG CCATGGAGTT TGGGATCACA 
AGTGATGATA TTTTCTGGCT CAAAGAGTCT CCCAAAAAAA CGTTGATCAT TGGTGCAAGC 
TATGTATCGC TGGAGTGTGC TGGTTTTCTT ACAGGCATTG GGCTGGACAC CAGTGTAATG 
GTGCGCAGTA TCGCCCTCCG CGGGTTTGAT CAGCAAATGT CTGGTTTAGT CACAGACTAC 
ATGGAGACGT ATGGCACGAA GTTCCACTGG AAGTGCACAC CGAAAAGTGT AGAAAAGCTG 
CCATCTGGCC ATCTTCAAGT CACATGGATG GATTTAAACA CCAAAGAGGA ACATCAAGAC 
ACATTCAACT CAGTACTTTG GGCTGTAGGT AAGGTCAGCA ATTCTAGAAT GCACACAAAA 
CATTTAATTT CATTGAATAA AAAAAAAACA TCAATGTCAA TCAAAGGTGG CAACACCAAG 
GGTCCAAAAA TACAAATTTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH1073-179P4.3 ( NM_001353396.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001340325.1 Danio rerio thioredoxin reductase 2, tandem duplicate 2 (txnrd2.2), transcript variant 1, mRNA.

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CloneID	ODa141160
Clone ID Related Accession (Same CDS sequence)	NM_001353396.1
Accession Version	NM_001353396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	thioredoxin reductase 2, mitochondrial isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CU651631.8 and CR847528.16. On Jul 11, 2017 this sequence version replaced XM_009301254.3. Transcript Variant: This variant (1) represents the predominant transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01008933.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCCT TCAGTAGAGG CAGGCAGAGG GTGAAAACTC TCTGCTGTTT CAGGACTTTA 
ATACTTTCAA GAAACTTTAC AGCAGGTAAA TTTGATTATG ATTTGGTGGT TATTGGTGGA 
GGCTCTGGAG GGTTGGCATG TTCAAAAGAA GCTGCCCAGT TAGGACAGAA AGTTGCTGTC 
TTGGACTATG TTGAACCTTC ACTCAAAGGC ACAAAGTGGG GTCTCGGTGG CACGTGTGTG 
AATGTCGGCT GTATTCCAAA GAAGCTTATG CATCAGGCAG CACTGCTTGG CACTGCTGTC 
AAAGATGCTA GGAAGTATGG CTGGCAAATC CCAGAAACCC TGTCACATGA CTGGCCAACA 
ATGGCTGAGG CTGTCCAGAA CCATGTTAGG TCTCTAAACT GGGGTCACAG AGTTCAACTA 
CAAGACAAGA AGGTGAAATA TTTAAATATG AAGGGCACTC TGCTGGATAA ACACACTGTC 
AGAGCTGTAA ATGCCCAAGG GAAGGAGATG ACAGTGACCG CCAGAAACAT TCTGTTAGCT 
ACTGGTGGAC GGCCAAAGTA CCCTACACAT GTCCCAGGTG CCATGGAGTT TGGGATCACA 
AGTGATGATA TTTTCTGGCT CAAAGAGTCT CCCAAAAAAA CGTTGATCAT TGGTGCAAGC 
TATGTATCGC TGGAGTGTGC TGGTTTTCTT ACAGGCATTG GGCTGGACAC CAGTGTAATG 
GTGCGCAGTA TCGCCCTCCG CGGGTTTGAT CAGCAAATGT CTGGTTTAGT CACAGACTAC 
ATGGAGACGT ATGGCACGAA GTTCCACTGG AAGTGCACAC CGAAAAGTGT AGAAAAGCTG 
CCATCTGGCC ATCTTCAAGT CACATGGATG GATTTAAACA CCAAAGAGGA ACATCAAGAC 
ACATTCAACT CAGTACTTTG GGCTGTAGGC AGAGCCCCTG AAACCAAGAC TCTTAATTTG 
GAAAAGGTTG GCGTGAAGAT CAACAAAGAA ACAGGAAAGA TAATCGTGGC TGCTGACGAA 
GCCACGTCTG TGCCGAATAT TTTTGCTATT GGAGACATCG GTGAGGGTCG TCCTGAGTTG 
ACCCCCACCG CCATTAAGGC TGGAAAACTC CTGGCCCACA GACTGGCTGG TCGTTCCACT 
GAGCTCATGA ATTACGAGAA TGTAGCTACT ACAGTGTTCA CACCACTAGA ATATGGCTGT 
GTAGGTCTGT CTGAAGAAGA GGCAGAAAGG AGACATGGAA AAGACCAGAT TGAGGTGTAC 
CATGCTTTCT ACAAACCGCT GGAGTTCACT GTGGCTGAGA GAGATGCCAC TCAATGCTAT 
ATAAAGGTGG TTTGTCTACG AGAAGGTGAC CAGCGCGTGC TGGGTCTGCA TTTTACTGGG 
CCGAACGCTG GTGAAGTCAC ACAGGGCTTT GCTTTGGGCT TCCAGTGTGG ACTTACATAC 
GAACACCTCA GGAACACAGT GGGGATTCAC CCCACCTGTG CTGAGGAGCT CACCAAACTC 
AACATCACCA AACGCTCGGG TCTTGACGCC ACGGTGACAG GCTGCTGAGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340325.1
CDS	253..1806
Translation	MAAFSRGRQRVKTLCCFRTLILSRNFTAGKFDYDLVVIGGGSGGLACSKEAAQLGQKVAVLDYVEPSLKGTKWGLGGTCVNVGCIPKKLMHQAALLGTAVKDARKYGWQIPETLSHDWPTMAEAVQNHVRSLNWGHRVQLQDKKVKYLNMKGTLLDKHTVRAVNAQGKEMTVTARNILLATGGRPKYPTHVPGAMEFGITSDDIFWLKESPKKTLIIGASYVSLECAGFLTGIGLDTSVMVRSIALRGFDQQMSGLVTDYMETYGTKFHWKCTPKSVEKLPSGHLQVTWMDLNTKEEHQDTFNSVLWAVGRAPETKTLNLEKVGVKINKETGKIIVAADEATSVPNIFAIGDIGEGRPELTPTAIKAGKLLAHRLAGRSTELMNYENVATTVFTPLEYGCVGLSEEEAERRHGKDQIEVYHAFYKPLEFTVAERDATQCYIKVVCLREGDQRVLGLHFTGPNAGEVTQGFALGFQCGLTYEHLRNTVGIHPTCAEELTKLNITKRSGLDATVTGC*G

Target ORF information:

RefSeq Version	NM_001353396.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio thioredoxin reductase 2, tandem duplicate 2 (txnrd2.2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353396.1

ORF Insert Sequence:

ATGGCTGCCT TCAGTAGAGG CAGGCAGAGG GTGAAAACTC TCTGCTGTTT CAGGACTTTA 
ATACTTTCAA GAAACTTTAC AGCAGGTAAA TTTGATTATG ATTTGGTGGT TATTGGTGGA 
GGCTCTGGAG GGTTGGCATG TTCAAAAGAA GCTGCCCAGT TAGGACAGAA AGTTGCTGTC 
TTGGACTATG TTGAACCTTC ACTCAAAGGC ACAAAGTGGG GTCTCGGTGG CACGTGTGTG 
AATGTCGGCT GTATTCCAAA GAAGCTTATG CATCAGGCAG CACTGCTTGG CACTGCTGTC 
AAAGATGCTA GGAAGTATGG CTGGCAAATC CCAGAAACCC TGTCACATGA CTGGCCAACA 
ATGGCTGAGG CTGTCCAGAA CCATGTTAGG TCTCTAAACT GGGGTCACAG AGTTCAACTA 
CAAGACAAGA AGGTGAAATA TTTAAATATG AAGGGCACTC TGCTGGATAA ACACACTGTC 
AGAGCTGTAA ATGCCCAAGG GAAGGAGATG ACAGTGACCG CCAGAAACAT TCTGTTAGCT 
ACTGGTGGAC GGCCAAAGTA CCCTACACAT GTCCCAGGTG CCATGGAGTT TGGGATCACA 
AGTGATGATA TTTTCTGGCT CAAAGAGTCT CCCAAAAAAA CGTTGATCAT TGGTGCAAGC 
TATGTATCGC TGGAGTGTGC TGGTTTTCTT ACAGGCATTG GGCTGGACAC CAGTGTAATG 
GTGCGCAGTA TCGCCCTCCG CGGGTTTGAT CAGCAAATGT CTGGTTTAGT CACAGACTAC 
ATGGAGACGT ATGGCACGAA GTTCCACTGG AAGTGCACAC CGAAAAGTGT AGAAAAGCTG 
CCATCTGGCC ATCTTCAAGT CACATGGATG GATTTAAACA CCAAAGAGGA ACATCAAGAC 
ACATTCAACT CAGTACTTTG GGCTGTAGGC AGAGCCCCTG AAACCAAGAC TCTTAATTTG 
GAAAAGGTTG GCGTGAAGAT CAACAAAGAA ACAGGAAAGA TAATCGTGGC TGCTGACGAA 
GCCACGTCTG TGCCGAATAT TTTTGCTATT GGAGACATCG GTGAGGGTCG TCCTGAGTTG 
ACCCCCACCG CCATTAAGGC TGGAAAACTC CTGGCCCACA GACTGGCTGG TCGTTCCACT 
GAGCTCATGA ATTACGAGAA TGTAGCTACT ACAGTGTTCA CACCACTAGA ATATGGCTGT 
GTAGGTCTGT CTGAAGAAGA GGCAGAAAGG AGACATGGAA AAGACCAGAT TGAGGTGTAC 
CATGCTTTCT ACAAACCGCT GGAGTTCACT GTGGCTGAGA GAGATGCCAC TCAATGCTAT 
ATAAAGGTGG TTTGTCTACG AGAAGGTGAC CAGCGCGTGC TGGGTCTGCA TTTTACTGGG 
CCGAACGCTG GTGAAGTCAC ACAGGGCTTT GCTTTGGGCT TCCAGTGTGG ACTTACATAC 
GAACACCTCA GGAACACAGT GGGGATTCAC CCCACCTGTG CTGAGGAGCT CACCAAACTC 
AACATCACCA AACGCTCGGG TCTTGACGCC ACGGTGACAG GCTGCTGAGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH1073-179P4.3 ( NM_001353394.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001340323.1 Danio rerio thioredoxin reductase 2, tandem duplicate 2 (txnrd2.2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa141161
Clone ID Related Accession (Same CDS sequence)	NM_001353394.1
Accession Version	NM_001353394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	thioredoxin reductase 2, mitochondrial isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CU651631.8 and CR847528.16. On Jul 12, 2017 this sequence version replaced XM_009301255.3. Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site at an internal exon compared to variant 1. The resulting isoform (2) is 1 aa shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCCT TCAGTAGAGG CAGGCAGAGG GTGAAAACTC TCTGCTGTTT CAGGACTTTA 
ATACTTTCAA GAAACTTTAC AGGTAAATTT GATTATGATT TGGTGGTTAT TGGTGGAGGC 
TCTGGAGGGT TGGCATGTTC AAAAGAAGCT GCCCAGTTAG GACAGAAAGT TGCTGTCTTG 
GACTATGTTG AACCTTCACT CAAAGGCACA AAGTGGGGTC TCGGTGGCAC GTGTGTGAAT 
GTCGGCTGTA TTCCAAAGAA GCTTATGCAT CAGGCAGCAC TGCTTGGCAC TGCTGTCAAA 
GATGCTAGGA AGTATGGCTG GCAAATCCCA GAAACCCTGT CACATGACTG GCCAACAATG 
GCTGAGGCTG TCCAGAACCA TGTTAGGTCT CTAAACTGGG GTCACAGAGT TCAACTACAA 
GACAAGAAGG TGAAATATTT AAATATGAAG GGCACTCTGC TGGATAAACA CACTGTCAGA 
GCTGTAAATG CCCAAGGGAA GGAGATGACA GTGACCGCCA GAAACATTCT GTTAGCTACT 
GGTGGACGGC CAAAGTACCC TACACATGTC CCAGGTGCCA TGGAGTTTGG GATCACAAGT 
GATGATATTT TCTGGCTCAA AGAGTCTCCC AAAAAAACGT TGATCATTGG TGCAAGCTAT 
GTATCGCTGG AGTGTGCTGG TTTTCTTACA GGCATTGGGC TGGACACCAG TGTAATGGTG 
CGCAGTATCG CCCTCCGCGG GTTTGATCAG CAAATGTCTG GTTTAGTCAC AGACTACATG 
GAGACGTATG GCACGAAGTT CCACTGGAAG TGCACACCGA AAAGTGTAGA AAAGCTGCCA 
TCTGGCCATC TTCAAGTCAC ATGGATGGAT TTAAACACCA AAGAGGAACA TCAAGACACA 
TTCAACTCAG TACTTTGGGC TGTAGGCAGA GCCCCTGAAA CCAAGACTCT TAATTTGGAA 
AAGGTTGGCG TGAAGATCAA CAAAGAAACA GGAAAGATAA TCGTGGCTGC TGACGAAGCC 
ACGTCTGTGC CGAATATTTT TGCTATTGGA GACATCGGTG AGGGTCGTCC TGAGTTGACC 
CCCACCGCCA TTAAGGCTGG AAAACTCCTG GCCCACAGAC TGGCTGGTCG TTCCACTGAG 
CTCATGAATT ACGAGAATGT AGCTACTACA GTGTTCACAC CACTAGAATA TGGCTGTGTA 
GGTCTGTCTG AAGAAGAGGC AGAAAGGAGA CATGGAAAAG ACCAGATTGA GGTGTACCAT 
GCTTTCTACA AACCGCTGGA GTTCACTGTG GCTGAGAGAG ATGCCACTCA ATGCTATATA 
AAGGTGGTTT GTCTACGAGA AGGTGACCAG CGCGTGCTGG GTCTGCATTT TACTGGGCCG 
AACGCTGGTG AAGTCACACA GGGCTTTGCT TTGGGCTTCC AGTGTGGACT TACATACGAA 
CACCTCAGGA ACACAGTGGG GATTCACCCC ACCTGTGCTG AGGAGCTCAC CAAACTCAAC 
ATCACCAAAC GCTCGGGTCT TGACGCCACG GTGACAGGCT GCTGAGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340323.1
CDS	253..1803
Translation	MAAFSRGRQRVKTLCCFRTLILSRNFTGKFDYDLVVIGGGSGGLACSKEAAQLGQKVAVLDYVEPSLKGTKWGLGGTCVNVGCIPKKLMHQAALLGTAVKDARKYGWQIPETLSHDWPTMAEAVQNHVRSLNWGHRVQLQDKKVKYLNMKGTLLDKHTVRAVNAQGKEMTVTARNILLATGGRPKYPTHVPGAMEFGITSDDIFWLKESPKKTLIIGASYVSLECAGFLTGIGLDTSVMVRSIALRGFDQQMSGLVTDYMETYGTKFHWKCTPKSVEKLPSGHLQVTWMDLNTKEEHQDTFNSVLWAVGRAPETKTLNLEKVGVKINKETGKIIVAADEATSVPNIFAIGDIGEGRPELTPTAIKAGKLLAHRLAGRSTELMNYENVATTVFTPLEYGCVGLSEEEAERRHGKDQIEVYHAFYKPLEFTVAERDATQCYIKVVCLREGDQRVLGLHFTGPNAGEVTQGFALGFQCGLTYEHLRNTVGIHPTCAEELTKLNITKRSGLDATVTGC*G

Target ORF information:

RefSeq Version	NM_001353394.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio thioredoxin reductase 2, tandem duplicate 2 (txnrd2.2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353394.1

ORF Insert Sequence:

ATGGCTGCCT TCAGTAGAGG CAGGCAGAGG GTGAAAACTC TCTGCTGTTT CAGGACTTTA 
ATACTTTCAA GAAACTTTAC AGGTAAATTT GATTATGATT TGGTGGTTAT TGGTGGAGGC 
TCTGGAGGGT TGGCATGTTC AAAAGAAGCT GCCCAGTTAG GACAGAAAGT TGCTGTCTTG 
GACTATGTTG AACCTTCACT CAAAGGCACA AAGTGGGGTC TCGGTGGCAC GTGTGTGAAT 
GTCGGCTGTA TTCCAAAGAA GCTTATGCAT CAGGCAGCAC TGCTTGGCAC TGCTGTCAAA 
GATGCTAGGA AGTATGGCTG GCAAATCCCA GAAACCCTGT CACATGACTG GCCAACAATG 
GCTGAGGCTG TCCAGAACCA TGTTAGGTCT CTAAACTGGG GTCACAGAGT TCAACTACAA 
GACAAGAAGG TGAAATATTT AAATATGAAG GGCACTCTGC TGGATAAACA CACTGTCAGA 
GCTGTAAATG CCCAAGGGAA GGAGATGACA GTGACCGCCA GAAACATTCT GTTAGCTACT 
GGTGGACGGC CAAAGTACCC TACACATGTC CCAGGTGCCA TGGAGTTTGG GATCACAAGT 
GATGATATTT TCTGGCTCAA AGAGTCTCCC AAAAAAACGT TGATCATTGG TGCAAGCTAT 
GTATCGCTGG AGTGTGCTGG TTTTCTTACA GGCATTGGGC TGGACACCAG TGTAATGGTG 
CGCAGTATCG CCCTCCGCGG GTTTGATCAG CAAATGTCTG GTTTAGTCAC AGACTACATG 
GAGACGTATG GCACGAAGTT CCACTGGAAG TGCACACCGA AAAGTGTAGA AAAGCTGCCA 
TCTGGCCATC TTCAAGTCAC ATGGATGGAT TTAAACACCA AAGAGGAACA TCAAGACACA 
TTCAACTCAG TACTTTGGGC TGTAGGCAGA GCCCCTGAAA CCAAGACTCT TAATTTGGAA 
AAGGTTGGCG TGAAGATCAA CAAAGAAACA GGAAAGATAA TCGTGGCTGC TGACGAAGCC 
ACGTCTGTGC CGAATATTTT TGCTATTGGA GACATCGGTG AGGGTCGTCC TGAGTTGACC 
CCCACCGCCA TTAAGGCTGG AAAACTCCTG GCCCACAGAC TGGCTGGTCG TTCCACTGAG 
CTCATGAATT ACGAGAATGT AGCTACTACA GTGTTCACAC CACTAGAATA TGGCTGTGTA 
GGTCTGTCTG AAGAAGAGGC AGAAAGGAGA CATGGAAAAG ACCAGATTGA GGTGTACCAT 
GCTTTCTACA AACCGCTGGA GTTCACTGTG GCTGAGAGAG ATGCCACTCA ATGCTATATA 
AAGGTGGTTT GTCTACGAGA AGGTGACCAG CGCGTGCTGG GTCTGCATTT TACTGGGCCG 
AACGCTGGTG AAGTCACACA GGGCTTTGCT TTGGGCTTCC AGTGTGGACT TACATACGAA 
CACCTCAGGA ACACAGTGGG GATTCACCCC ACCTGTGCTG AGGAGCTCAC CAAACTCAAC 
ATCACCAAAC GCTCGGGTCT TGACGCCACG GTGACAGGCT GCTGAGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Selenoprotein Gene Nomenclature.
The Journal of biological chemistry291(46)24036-24040(2016 Nov)
Gladyshev VN,Arn?r ES,Berry MJ,Brigelius-Floh? R,Bruford EA,Burk RF,Carlson BA,Castellano S,Chavatte L,Conrad M,Copeland PR,Diamond AM,Driscoll DM,Ferreiro A,Floh? L,Green FR,Guig? R,Handy DE,Hatfield DL,Hesketh J,Hoffmann PR,Holmgren A,Hondal RJ,Howard MT,Huang K,Kim HY,Kim IY,K?hrle J,Krol A,Kryukov GV,Lee BJ,Lee BC,Lei XG,Liu Q,Lescure A,Lobanov AV,Loscalzo J,Maiorino M,Mariotti M,Sandeep Prabhu K,Rayman MP,Rozovsky S,Salinas G,Schmidt EE,Schomburg L,Schweizer U,Simonovi? M,Sunde RA,Tsuji PA,Tweedie S,Ursini F,Whanger PD,Zhang Y

Comparative mapping of the 22q11.2 deletion region and the potential of simple model organisms.
Journal of neurodevelopmental disorders7(1)18(2015)
Guna A,Butcher NJ,Bassett AS

Selenium prevents downregulation of antioxidant selenoprotein genes by methylmercury.
Free radical biology & medicine7595-104(2014 Oct)
Penglase S,Hamre K,Ellingsen S

Composition and evolution of the vertebrate and mammalian selenoproteomes.
PloS one7(3)e33066(2012)
Mariotti M,Ridge PG,Zhang Y,Lobanov AV,Pringle TH,Guigo R,Hatfield DL,Gladyshev VN

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SI:CH1073-179P4.3 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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