eef2a.1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	eef2a.1
Entrez Gene ID	792182
Full Name	eukaryotic translation elongation factor 2a, tandem duplicate 1
Synonyms	si:dkey-110c1.3,zgc:113191
General protein information	Preferred Names eukaryotic translation elongation factor 2a, tandem duplicate 1 Names eukaryotic translation elongation factor 2a, tandem duplicate 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017353196.1	XP_017208685.1	eukaryotic translation elongation factor 2a, tandem duplicate 1 isoform X1
NM_001045161.2	NP_001038626.2	eukaryotic translation elongation factor 2a, tandem duplicate 1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	eef2a.1	NP_001038626.2
Danio rerio (zebrafish)	eef2a.2	NP_001038637.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Ghrelin O-Acyl Transferase in Zebrafish Is an Evolutionarily Conserved Peptide Upregulated During Calorie Restriction.
Hatef A, Yufa R, Unniappan S
Zebrafish12(5)327-38(2015 Oct)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Nutrient status modulates the expression of nesfatin-1 encoding nucleobindin 2A and 2B mRNAs in zebrafish gut, liver and brain.
Hatef A, Shajan S, Unniappan S
General and comparative endocrinology21551-60(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following eef2a.1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eef2a.1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa34582	NM_001045161.2 Latest version!	Danio rerio eukaryotic translation elongation factor 2a, tandem duplicate 1 (eef2a.1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa57067	XM_017353196.1 Latest version!	Danio rerio eukaryotic translation elongation factor 2a, tandem duplicate 1 (eef2a.1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eef2a.1 ( NM_001045161.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038626.2 Danio rerio eukaryotic translation elongation factor 2a, tandem duplicate 1 (eef2a.1), mRNA.

Price & Availability↑

CloneID	ODa34582
Clone ID Related Accession (Same CDS sequence)	NM_001045161.2
Accession Version	NM_001045161.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	eukaryotic translation elongation factor 2a, tandem duplicate 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX005203.8. On or before Mar 15, 2008 this sequence version replaced NM_001020717.1, NM_001045161.1. ##Evidence-Data-START## Transcript exon combination :: GFIL01009235.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA898400 [ECO:0000348] ##Evidence-Data-END##

ATGGAGAACT TCAATGTAGA CCAGATCCGT GCCACCATGG ACAAGAAGTC CAACATCCGT 
AACATGTCTG TAATAGGAGC TTTTGATCAT GGCAAATCAA CTTTGACCGA TTGGCTGGTG 
TCAGAAGCCG GTATCGTCAG CTCAGCCCGT GCTGGAGAAA CCCGTTTCAT GGATACGAGG 
CGTGATGAAC AAGAGCGTTG CATCACCATC AAGTCAACGG CCATCTCCAT ATTCTATGAG 
CTTGCTGAAA AGGATTTGGC TTTCATTAAA GAGTGCAAAG ATGGCTCCGG CTTCCTCCTC 
AACCTGATTG ACTCCCCTGG GCATGTTGAC TTTTCTTCAG AAGTTACAGC TGCACTGCGT 
ATCACAGATG GAGCATTGCT GGTGGTGGAT TGTGTGTCTG GTGTGTGCTT ACAGACAGAG 
ACTGTGCTCA GACAGGCCAT TGGTGAGAGG ATTAAACCAG TTTTGATGAT TAACAAAATG 
GATCGAGCTT TGCTCGAGCT ACAGTTGGTC CCTGAAGAGC TGTACCAGAT CTTTCAGCGC 
ATTGTTGAGA AAGTCAACGT GACCATCTCC ACCTATGCTG AGGATGAGAA AGGCCCTATG 
GGCAATGTCA TGATTGATCC TGTCATTGGT AATCTTGCCT TTGGATCTGG ACTGCATGGA 
TGGGCCTTCA CTTTGAAGCA GTTTGCTGAG CTCTATGTGA AAAAGTTCGC TGGTAAAGCT 
CAGCTGGGAC CGGAAGAGTA CATCAAGAAG GTGGAGGACA TGATAAAGAG ACTTTGGGGC 
GACAGCTACT TTGACTCTAC TACTGGCAAG TTCAGCGAGT CTGCGACATC GCCAGATGGC 
AAGAAGCTTC CCAGGACTTT TGTTCACTTA GTCCTGGACC CCATCTTCAA AGTATTTGAT 
GCCATCATGA ACTTCAAAAA GGAGGAAACT GTAAAGCTGA TTGAGAAGAT GGGCATCAAG 
CTAGATGTGG AGGACAAGGA GAAGGAGGGC AAAATCCTAC TGAAGGCTGT GATGCGTTGC 
TGGCTCCCGG CTGGAGAGGC TCTGCTTCAG ATGATCACCA TCCACCTGCC CTCACCGGTT 
ACTGCTCAGA TTTACCGCTG TGAGCTGCTG TATGAAGGAC CTGGAGATGA TGAGGCTGCC 
ATGGGCATCA AGAATTGTGA TCCTAAAGCA CCTCTCATGA TGTACATTTC TAAAATGATA 
CCAACATCTG ACAAAGGTTG TTTTTATGCG TTTGGTCGCG TCTTCTCTGG CTGTGTCTCC 
ACTGGCCTGA ATGTGCGCAT TATGGGGCCC AACTTCACCC CTGGAAAAAA GGATGATCTT 
TACCTGAAAC CCATCCAAAG GACCGTTCTA ATGATGGGTC GCTACGTTGA ACCCATTGAA 
GATGTGCCCT GCGGCAATAT TGTAGGTCTG TTAGGGGTGG ATCAGTTTTT GGTAAAGACT 
GGAACCATCA CTACTTTCAC AAAAGCCTAC AATATGCGTG TGATGAAGTT TAGTGTCAGT 
CCTGTGGTGA GAGTGTCCGT GGAGGTCATG GACCCTGCTG ACCTTCCTAA ACTGGTGGAG 
GGATTGAAGC ATCTGGCCAA GTCAGATCCC ATGTTGCAAT GCATCATTGA GGATACTGGT 
GAACATATCA TTGCTGGAGC TGGTGAGCTT CACTTGGAGA TTTGCCTTAA GGATCTTGAA 
GAGGACCATG CCTGCATTCC CCTCAAGAAA TCAGATCCAT TTGCTTCCTA CAGAGAGACG 
GTCAGTGATG GCTCCAAACA ATTGTGCCTT GCCAAGACGC CAAACAAGCA CAGTCGTCTC 
TTCATGAAGG CCTGTCCTCT GGCTGATGGT CTTGCAGAAG ATATTGATAA GGGTCGGGTC 
ACTGCCAGCC AGGAGATGAA TGCCCGTGCC CAATACCTTG CTGAAAACTA TAAGTGGGAA 
GTGACTGAAG CTCGTAAGAT CTGGTGCTTT GGCCCTGAGG GAACTGGACC CAACATCTTG 
GTAGATTTGA CCAAGAGAGT GCAATACTTG AATGAGATTA AGGACAGTGT GATTGCTGGA 
TTTCAGTGGG CCACTAGAGA GGGAGTCTTG TGCGCAGAGA ACATGCGTGG CATTCGCTTT 
GACATTCATG ATGCAACTTT GACTTCTACT GCCATTCACC GTGGTCCAGG TCAAATAATT 
GTTGCAACCC GCCGAGTGCT TTATGGCTGT CAGCTCACAG CTGAACCAAG GCTTAGTGAG 
CCGATATACC TGGTGGAGAT GCAGTGCCCA GAGTCGGTTA TTGGTAACGT CTATGGTGAA 
CTGGTCAGGA GGCGTGGAGT AGTGTTTTCA GAGTCCCAGG TTATGGGAAC TCCTGTTTAT 
TTGCTCAAGG CTTACTTGCC TGTCAGCGAG TCATTTGGTT TTACAGCTGA CCTTTGTGCC 
AACACATCTG GCCAAGCCTT CTCACAGTGT GTGTTTGATC ATTGGCAGAT ATTGCCTGGA 
GACCCAATGG ATCCTACCTC CAAAGTTGCT CATATTATGG CTGATATACG AAAGAGCAAG 
GGCCTTGATG AAGCCATTCC AGCATTACAT TGCTACTTGG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038626.2
CDS	35..2599
Translation	MENFNVDQIRATMDKKSNIRNMSVIGAFDHGKSTLTDWLVSEAGIVSSARAGETRFMDTRRDEQERCITIKSTAISIFYELAEKDLAFIKECKDGSGFLLNLIDSPGHVDFSSEVTAALRITDGALLVVDCVSGVCLQTETVLRQAIGERIKPVLMINKMDRALLELQLVPEELYQIFQRIVEKVNVTISTYAEDEKGPMGNVMIDPVIGNLAFGSGLHGWAFTLKQFAELYVKKFAGKAQLGPEEYIKKVEDMIKRLWGDSYFDSTTGKFSESATSPDGKKLPRTFVHLVLDPIFKVFDAIMNFKKEETVKLIEKMGIKLDVEDKEKEGKILLKAVMRCWLPAGEALLQMITIHLPSPVTAQIYRCELLYEGPGDDEAAMGIKNCDPKAPLMMYISKMIPTSDKGCFYAFGRVFSGCVSTGLNVRIMGPNFTPGKKDDLYLKPIQRTVLMMGRYVEPIEDVPCGNIVGLLGVDQFLVKTGTITTFTKAYNMRVMKFSVSPVVRVSVEVMDPADLPKLVEGLKHLAKSDPMLQCIIEDTGEHIIAGAGELHLEICLKDLEEDHACIPLKKSDPFASYRETVSDGSKQLCLAKTPNKHSRLFMKACPLADGLAEDIDKGRVTASQEMNARAQYLAENYKWEVTEARKIWCFGPEGTGPNILVDLTKRVQYLNEIKDSVIAGFQWATREGVLCAENMRGIRFDIHDATLTSTAIHRGPGQIIVATRRVLYGCQLTAEPRLSEPIYLVEMQCPESVIGNVYGELVRRRGVVFSESQVMGTPVYLLKAYLPVSESFGFTADLCANTSGQAFSQCVFDHWQILPGDPMDPTSKVAHIMADIRKSKGLDEAIPALHCYLD

Target ORF information:

RefSeq Version	NM_001045161.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio eukaryotic translation elongation factor 2a, tandem duplicate 1 (eef2a.1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045161.2

ORF Insert Sequence:

ATGGAGAACT TCAATGTAGA CCAGATCCGT GCCACCATGG ACAAGAAGTC CAACATCCGT 
AACATGTCTG TAATAGGAGC TTTTGATCAT GGCAAATCAA CTTTGACCGA TTGGCTGGTG 
TCAGAAGCCG GTATCGTCAG CTCAGCCCGT GCTGGAGAAA CCCGTTTCAT GGATACGAGG 
CGTGATGAAC AAGAGCGTTG CATCACCATC AAGTCAACGG CCATCTCCAT ATTCTATGAG 
CTTGCTGAAA AGGATTTGGC TTTCATTAAA GAGTGCAAAG ATGGCTCCGG CTTCCTCCTC 
AACCTGATTG ACTCCCCTGG GCATGTTGAC TTTTCTTCAG AAGTTACAGC TGCACTGCGT 
ATCACAGATG GAGCATTGCT GGTGGTGGAT TGTGTGTCTG GTGTGTGCTT ACAGACAGAG 
ACTGTGCTCA GACAGGCCAT TGGTGAGAGG ATTAAACCAG TTTTGATGAT TAACAAAATG 
GATCGAGCTT TGCTCGAGCT ACAGTTGGTC CCTGAAGAGC TGTACCAGAT CTTTCAGCGC 
ATTGTTGAGA AAGTCAACGT GACCATCTCC ACCTATGCTG AGGATGAGAA AGGCCCTATG 
GGCAATGTCA TGATTGATCC TGTCATTGGT AATCTTGCCT TTGGATCTGG ACTGCATGGA 
TGGGCCTTCA CTTTGAAGCA GTTTGCTGAG CTCTATGTGA AAAAGTTCGC TGGTAAAGCT 
CAGCTGGGAC CGGAAGAGTA CATCAAGAAG GTGGAGGACA TGATAAAGAG ACTTTGGGGC 
GACAGCTACT TTGACTCTAC TACTGGCAAG TTCAGCGAGT CTGCGACATC GCCAGATGGC 
AAGAAGCTTC CCAGGACTTT TGTTCACTTA GTCCTGGACC CCATCTTCAA AGTATTTGAT 
GCCATCATGA ACTTCAAAAA GGAGGAAACT GTAAAGCTGA TTGAGAAGAT GGGCATCAAG 
CTAGATGTGG AGGACAAGGA GAAGGAGGGC AAAATCCTAC TGAAGGCTGT GATGCGTTGC 
TGGCTCCCGG CTGGAGAGGC TCTGCTTCAG ATGATCACCA TCCACCTGCC CTCACCGGTT 
ACTGCTCAGA TTTACCGCTG TGAGCTGCTG TATGAAGGAC CTGGAGATGA TGAGGCTGCC 
ATGGGCATCA AGAATTGTGA TCCTAAAGCA CCTCTCATGA TGTACATTTC TAAAATGATA 
CCAACATCTG ACAAAGGTTG TTTTTATGCG TTTGGTCGCG TCTTCTCTGG CTGTGTCTCC 
ACTGGCCTGA ATGTGCGCAT TATGGGGCCC AACTTCACCC CTGGAAAAAA GGATGATCTT 
TACCTGAAAC CCATCCAAAG GACCGTTCTA ATGATGGGTC GCTACGTTGA ACCCATTGAA 
GATGTGCCCT GCGGCAATAT TGTAGGTCTG TTAGGGGTGG ATCAGTTTTT GGTAAAGACT 
GGAACCATCA CTACTTTCAC AAAAGCCTAC AATATGCGTG TGATGAAGTT TAGTGTCAGT 
CCTGTGGTGA GAGTGTCCGT GGAGGTCATG GACCCTGCTG ACCTTCCTAA ACTGGTGGAG 
GGATTGAAGC ATCTGGCCAA GTCAGATCCC ATGTTGCAAT GCATCATTGA GGATACTGGT 
GAACATATCA TTGCTGGAGC TGGTGAGCTT CACTTGGAGA TTTGCCTTAA GGATCTTGAA 
GAGGACCATG CCTGCATTCC CCTCAAGAAA TCAGATCCAT TTGCTTCCTA CAGAGAGACG 
GTCAGTGATG GCTCCAAACA ATTGTGCCTT GCCAAGACGC CAAACAAGCA CAGTCGTCTC 
TTCATGAAGG CCTGTCCTCT GGCTGATGGT CTTGCAGAAG ATATTGATAA GGGTCGGGTC 
ACTGCCAGCC AGGAGATGAA TGCCCGTGCC CAATACCTTG CTGAAAACTA TAAGTGGGAA 
GTGACTGAAG CTCGTAAGAT CTGGTGCTTT GGCCCTGAGG GAACTGGACC CAACATCTTG 
GTAGATTTGA CCAAGAGAGT GCAATACTTG AATGAGATTA AGGACAGTGT GATTGCTGGA 
TTTCAGTGGG CCACTAGAGA GGGAGTCTTG TGCGCAGAGA ACATGCGTGG CATTCGCTTT 
GACATTCATG ATGCAACTTT GACTTCTACT GCCATTCACC GTGGTCCAGG TCAAATAATT 
GTTGCAACCC GCCGAGTGCT TTATGGCTGT CAGCTCACAG CTGAACCAAG GCTTAGTGAG 
CCGATATACC TGGTGGAGAT GCAGTGCCCA GAGTCGGTTA TTGGTAACGT CTATGGTGAA 
CTGGTCAGGA GGCGTGGAGT AGTGTTTTCA GAGTCCCAGG TTATGGGAAC TCCTGTTTAT 
TTGCTCAAGG CTTACTTGCC TGTCAGCGAG TCATTTGGTT TTACAGCTGA CCTTTGTGCC 
AACACATCTG GCCAAGCCTT CTCACAGTGT GTGTTTGATC ATTGGCAGAT ATTGCCTGGA 
GACCCAATGG ATCCTACCTC CAAAGTTGCT CATATTATGG CTGATATACG AAAGAGCAAG 
GGCCTTGATG AAGCCATTCC AGCATTACAT TGCTACTTGG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eef2a.1 ( XM_017353196.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208685.1 Danio rerio eukaryotic translation elongation factor 2a, tandem duplicate 1 (eef2a.1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa57067
Clone ID Related Accession (Same CDS sequence)	XM_017353196.1
Accession Version	XM_017353196.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	eukaryotic translation elongation factor 2a, tandem duplicate 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACT TCAATGTAGA CCAGATCCGT GCCACCATGG ACAAGAAGTC CAACATCCGT 
AACATGTCTG TAATAGGAGC TTTTGATCAT GGCAAATCAA CTTTGACCGA TTGGCTGGTG 
TCAGAAGCCG GTATCGTCAG CTCAGCCCGT GCTGGAGAAA CCCGTTTCAT GGATACGAGG 
CGTGATGAAC AAGAGCGTTG CATCACCATC AAGTCAACGG CCATCTCCAT ATTCTATGAG 
CTTGCTGAAA AGGATTTGGC TTTCATTAAA GAGTGCAAAG ATGGCTCCGG CTTCCTCCTC 
AACCTGATTG ACTCCCCTGG GCATGTTGAC TTTTCTTCAG AAGTTACAGC TGCACTGCGT 
ATCACAGATG GAGCATTGCT GGTGGTGGAT TGTGTGTCTG GTGTGTGCTT ACAGACAGAG 
ACTGTGCTCA GACAGGCCAT TGGTGAGAGG ATTAAACCAG TTTTGATGAT TAACAAAATG 
GATCGAGCTT TGCTCGAGCT ACAGTTGGTC CCTGAAGAGC TGTACCAGAT CTTTCAGCGC 
ATTGTTGAGA AAGTCAACGT GACCATCTCC ACCTATGCTG AGGATGAGAA AGGCCCTATG 
GGCAATGTCA TGATTGATCC TGTCATTGGT AATCTTGCCT TTGGATCTGG ACTGCATGGA 
TGGGCCTTCA CTTTGAAGCA GTTTGCTGAG CTCTATGTGA AAAAGTTCGC TGGTAAAGCT 
CAGCTGGGAC CGGAAGAGTA CATCAAGAAG GTGGAGGACA TGATAAAGAG ACTTTGGGGC 
GACAGCTACT TTGACTCTAC TACTGGCAAG TTCAGCGAGT CTGCGACATC GCCAGATGGC 
AAGAAGCTTC CCAGGACTTT TGTTCACTTA GTCCTGGACC CCATCTTCAA AGTATTTGAT 
GCCATCATGA ACTTCAAAAA GGAGGAAACT GTAAAGCTGA TTGAGAAGAT GGGCATCAAG 
CTAGATGTGG AGGACAAGGA GAAGGAGGGC AAAATCCTAC TGAAGGCTGT GATGCGTTGC 
TGGCTCCCGG CTGGAGAGGC TCTGCTTCAG ATGATCACCA TCCACCTGCC CTCACCGGTT 
ACTGCTCAGA TTTACCGCTG TGAGCTGCTG TATGAAGGAC CTGGAGATGA TGAGGCTGCC 
ATGGGCATCA AGAATTGTGA TCCTAAAGCA CCTCTCATGA TGTACATTTC TAAAATGATA 
CCAACATCTG ACAAAGGTTG TTTTTATGCG TTTGGTCGCG TCTTCTCTGG CTGTGTCTCC 
ACTGGCCTGA ATGTGCGCAT TATGGGGCCC AACTTCACCC CTGGAAAAAA GGATGATCTT 
TACCTGAAAC CCATCCAAAG GACCGTTCTA ATGATGGGTC GCTACGTTGA ACCCATTGAA 
GATGTGCCCT GCGGCAATAT TGTAGGTCTG TTAGGGGTGG ATCAGTTTTT GGTAAAGACT 
GGAACCATCA CTACTTTCAC AAAAGCCTAC AATATGCGTG TGATGAAGTT TAGTGTCAGT 
CCTGTGGTGA GAGTGTCCGT GGAGGTCATG GACCCTGCTG ACCTTCCTAA ACTGGTGGAG 
GGATTGAAGC ATCTGGCCAA GTCAGATCCC ATGTTGCAAT GCATCATTGA GGATACTGGT 
GAACATATCA TTGCTGGAGC TGGTGAGCTT CACTTGGAGA TTTGCCTTAA GGATCTTGAA 
GAGGACCATG CCTGCATTCC CCTCAAGAGA GACGGTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208685.1
CDS	77..1798
Translation	MENFNVDQIRATMDKKSNIRNMSVIGAFDHGKSTLTDWLVSEAGIVSSARAGETRFMDTRRDEQERCITIKSTAISIFYELAEKDLAFIKECKDGSGFLLNLIDSPGHVDFSSEVTAALRITDGALLVVDCVSGVCLQTETVLRQAIGERIKPVLMINKMDRALLELQLVPEELYQIFQRIVEKVNVTISTYAEDEKGPMGNVMIDPVIGNLAFGSGLHGWAFTLKQFAELYVKKFAGKAQLGPEEYIKKVEDMIKRLWGDSYFDSTTGKFSESATSPDGKKLPRTFVHLVLDPIFKVFDAIMNFKKEETVKLIEKMGIKLDVEDKEKEGKILLKAVMRCWLPAGEALLQMITIHLPSPVTAQIYRCELLYEGPGDDEAAMGIKNCDPKAPLMMYISKMIPTSDKGCFYAFGRVFSGCVSTGLNVRIMGPNFTPGKKDDLYLKPIQRTVLMMGRYVEPIEDVPCGNIVGLLGVDQFLVKTGTITTFTKAYNMRVMKFSVSPVVRVSVEVMDPADLPKLVEGLKHLAKSDPMLQCIIEDTGEHIIAGAGELHLEICLKDLEEDHACIPLKRDGQ

Target ORF information:

RefSeq Version	XM_017353196.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio eukaryotic translation elongation factor 2a, tandem duplicate 1 (eef2a.1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353196.1

ORF Insert Sequence:

ATGGAGAACT TCAATGTAGA CCAGATCCGT GCCACCATGG ACAAGAAGTC CAACATCCGT 
AACATGTCTG TAATAGGAGC TTTTGATCAT GGCAAATCAA CTTTGACCGA TTGGCTGGTG 
TCAGAAGCCG GTATCGTCAG CTCAGCCCGT GCTGGAGAAA CCCGTTTCAT GGATACGAGG 
CGTGATGAAC AAGAGCGTTG CATCACCATC AAGTCAACGG CCATCTCCAT ATTCTATGAG 
CTTGCTGAAA AGGATTTGGC TTTCATTAAA GAGTGCAAAG ATGGCTCCGG CTTCCTCCTC 
AACCTGATTG ACTCCCCTGG GCATGTTGAC TTTTCTTCAG AAGTTACAGC TGCACTGCGT 
ATCACAGATG GAGCATTGCT GGTGGTGGAT TGTGTGTCTG GTGTGTGCTT ACAGACAGAG 
ACTGTGCTCA GACAGGCCAT TGGTGAGAGG ATTAAACCAG TTTTGATGAT TAACAAAATG 
GATCGAGCTT TGCTCGAGCT ACAGTTGGTC CCTGAAGAGC TGTACCAGAT CTTTCAGCGC 
ATTGTTGAGA AAGTCAACGT GACCATCTCC ACCTATGCTG AGGATGAGAA AGGCCCTATG 
GGCAATGTCA TGATTGATCC TGTCATTGGT AATCTTGCCT TTGGATCTGG ACTGCATGGA 
TGGGCCTTCA CTTTGAAGCA GTTTGCTGAG CTCTATGTGA AAAAGTTCGC TGGTAAAGCT 
CAGCTGGGAC CGGAAGAGTA CATCAAGAAG GTGGAGGACA TGATAAAGAG ACTTTGGGGC 
GACAGCTACT TTGACTCTAC TACTGGCAAG TTCAGCGAGT CTGCGACATC GCCAGATGGC 
AAGAAGCTTC CCAGGACTTT TGTTCACTTA GTCCTGGACC CCATCTTCAA AGTATTTGAT 
GCCATCATGA ACTTCAAAAA GGAGGAAACT GTAAAGCTGA TTGAGAAGAT GGGCATCAAG 
CTAGATGTGG AGGACAAGGA GAAGGAGGGC AAAATCCTAC TGAAGGCTGT GATGCGTTGC 
TGGCTCCCGG CTGGAGAGGC TCTGCTTCAG ATGATCACCA TCCACCTGCC CTCACCGGTT 
ACTGCTCAGA TTTACCGCTG TGAGCTGCTG TATGAAGGAC CTGGAGATGA TGAGGCTGCC 
ATGGGCATCA AGAATTGTGA TCCTAAAGCA CCTCTCATGA TGTACATTTC TAAAATGATA 
CCAACATCTG ACAAAGGTTG TTTTTATGCG TTTGGTCGCG TCTTCTCTGG CTGTGTCTCC 
ACTGGCCTGA ATGTGCGCAT TATGGGGCCC AACTTCACCC CTGGAAAAAA GGATGATCTT 
TACCTGAAAC CCATCCAAAG GACCGTTCTA ATGATGGGTC GCTACGTTGA ACCCATTGAA 
GATGTGCCCT GCGGCAATAT TGTAGGTCTG TTAGGGGTGG ATCAGTTTTT GGTAAAGACT 
GGAACCATCA CTACTTTCAC AAAAGCCTAC AATATGCGTG TGATGAAGTT TAGTGTCAGT 
CCTGTGGTGA GAGTGTCCGT GGAGGTCATG GACCCTGCTG ACCTTCCTAA ACTGGTGGAG 
GGATTGAAGC ATCTGGCCAA GTCAGATCCC ATGTTGCAAT GCATCATTGA GGATACTGGT 
GAACATATCA TTGCTGGAGC TGGTGAGCTT CACTTGGAGA TTTGCCTTAA GGATCTTGAA 
GAGGACCATG CCTGCATTCC CCTCAAGAGA GACGGTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Ghrelin O-Acyl Transferase in Zebrafish Is an Evolutionarily Conserved Peptide Upregulated During Calorie Restriction.
Zebrafish12(5)327-38(2015 Oct)
Hatef A,Yufa R,Unniappan S

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Nutrient status modulates the expression of nesfatin-1 encoding nucleobindin 2A and 2B mRNAs in zebrafish gut, liver and brain.
General and comparative endocrinology21551-60(2015 May)
Hatef A,Shajan S,Unniappan S

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eef2a.1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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