impg2b cDNA ORF clone, Danio rerio(zebrafish)

The following impg2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the impg2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa146186 XM_021473522.1
Latest version!
Danio rerio interphotoreceptor matrix proteoglycan 2b (impg2b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa146186 XM_021469612.1
Latest version!
Danio rerio interphotoreceptor matrix proteoglycan 2b (impg2b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa146186 XM_021474486.1
Latest version!
Danio rerio interphotoreceptor matrix proteoglycan 2b (impg2b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa146187 XM_021474487.1
Latest version!
Danio rerio interphotoreceptor matrix proteoglycan 2b (impg2b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa146187 XM_021469613.1
Latest version!
Danio rerio interphotoreceptor matrix proteoglycan 2b (impg2b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa146187 XM_021473523.1
Latest version!
Danio rerio interphotoreceptor matrix proteoglycan 2b (impg2b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27099 XM_021474484.1
Latest version!
Danio rerio interphotoreceptor matrix proteoglycan 2b (impg2b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27099 XM_009296447.3
Latest version!
Danio rerio interphotoreceptor matrix proteoglycan 2b (impg2b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27099 XM_021473520.1
Latest version!
Danio rerio interphotoreceptor matrix proteoglycan 2b (impg2b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa45891 XM_021473521.1
Latest version!
Danio rerio interphotoreceptor matrix proteoglycan 2b (impg2b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa45891 XM_021474485.1
Latest version!
Danio rerio interphotoreceptor matrix proteoglycan 2b (impg2b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa45891 XM_009296448.3
Latest version!
Danio rerio interphotoreceptor matrix proteoglycan 2b (impg2b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
ODa27099 XM_009296447.2 Danio rerio interphotoreceptor matrix proteoglycan 2b (impg2b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa45891 XM_009296448.2 Danio rerio interphotoreceptor matrix proteoglycan 2b (impg2b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa146186
    Clone ID Related Accession (Same CDS sequence) XM_021473522.1 , XM_021469612.1 , XM_021474486.1
    Accession Version XM_021473522.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8340bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin homolog isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395115.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGCCTGATT TACCTTTTAA ATGCATTTGG TGGGGTTTGA CACTATTGTT GTTGACTGGA 
    TACTTTTCTA TAGATGCAGA TACTGGAACT CATGAGGATT ACTTGGCAAA TGGAAATACA
    ATGCTTTTGG ACACTAATGA AGTTTTGAAT CACTTATTGT TGTTGGACAA AGGTAAAACT
    TTGAATTACA TGTCTGCTGA AGGGAAAACA GCTCTATCTA GACATAAAAG GGACCTCCTG
    TTCCCCAATG GAGTTAAACT GTGCTCACAT GAGACGGTGC AACAGGCAGT GCAGAATCAT
    CTCAACTACT TTCACCTGCG AGTGTGCCAG GAGACAATTT GGGAGGCTTA TAAAATCTTC
    TGGGATCGCT TACCTGAGAA AGAAGAGTAC CAGATCTGGA TGAAAAAATG TCAAAATGGT
    AGCATTAGCG TGTTTGACAT CGGCCGAAGC TTTAGTCAGT CAGCTGAGCA CCTTGCTTTG
    ATTTCAAGTA GGGTAGCATC ATCCACAATA ACATCAACAA GTGCGCCCAC CAGCCCATGG
    CAACCTGAAT GCAGGCATCA GACGACGACT ACTTCGCCAA CACTAGCAAA AGCTGAACAA
    GTTGGTATTT TGATTCCTGA GGCAGTGCCA GGTTCACAAG TTGTCACACC GCAGACCTTT
    GCAGATGATA TCGTAATCAC AACTTTGCCT ACAAGGCTTC AAGTGACACC TGGAATTACC
    CCAAAGCCTG ATCTTGTCAC AGCCTTTAAG GACACAGTAA AACCTATTTC TGAAACTGAA
    CCTGAGGTCA TATTGGGTAC TACTTCTGAG AGTCCTGTTA AAGCATTGGA TACCATTACC
    ATTAAACATG AAGAGGTTGT TGTAGTCTCA GAATCTAAAT TATTGATTAC TGAGAGGACT
    TCGGAGGACT CTACTTCAAG GAAATATGAT TATGAGTTTC CCACGACCTT TAAGGACTCG
    TTAGTAATAA ACACTCCTAT AGTACTAACA ACTGAATTTT CTGAACAAAT GTATTCAGAA
    ATAGGACAAG GTTTTTCTGA TGGTACAGTT AAAACAGTAG CTGTTACTAT TCAAAAGGAA
    CTTGACATTG TCTCAAAATC TACATTATTA ACCACTGACA AAACTCCAGA AGTTTCTACT
    TCAAAAAAAT CTGAGCATGA TAATCCCACT AACTTTGTAG ATTCATTGGG AATAAGCACC
    AATACTCCTA GAGTGACCAC AAGTGAACTC AAAGAAGATC TTCATTCAGA ATTAGAACAG
    GTGGCTGGGC AGGATGTTAT TCACAGTCCT GTTAAAACAA CACATGTTAC AATTAAGCAT
    GAAGTGTTGG ACACAAATAC TACATTAATG ACAACTGACA GAACTCCATA TTCTACTTCA
    GCAAAATATG AACGTGTTAT ACCAACATCC TTTGAGGACT CACTAGTCAC AAGTTCATCT
    ACTGAACAGG ATATGACCAA TGTTCCTATT AAGACGGCAT ATGCCACAAT TTCACATGGA
    GAGTCTGAAA TTGTCACAGA GTCGACAATA ACAACCACTA ACAGAAATCT TGAAGATTCT
    TCTTTAGCAA AATATGATTA TGGGATTCCT CAAGGAATTG ATACTTCTTC GGGAGTAATT
    AATGAATCTT CTACAAAAGA ACATAATTCA GTATCTAAAT TGAATATTGG TCAGACTACT
    ACTGAAGGTC CCCAAACAAC TGATGCAGTT CAGTATGTAG AATCTGACAT TGTGTCAGAG
    GAAACGTTGA AATCCACTGG CAGAACTGCA GTTAGCTCAA ACTCTACAAA AATTGAGGCC
    GATCTTTCCA CAACTCCTGA GGACCTGACA GGAGTAAGTA CCAACACTCC TGAAATAGTC
    ACTAGAGATT CCATTACAAT ACTTATCCCA GAAACCGAGG GCATTACTAA AAATCCTGTT
    AAATCTGCAC CTATTACCAT TCAGCATGTG GATGTTGATG ATGTTTTAGC AGCTACAGTG
    ATGTCCACTC ATAAACCTCC CAAAGTCTCA CCTACAGAAA TATCCCCCGA TCAGAATCTC
    TTACCAGAGG AATCAAAGCT GCCAACTCCT ACAAAACTAG CAGAAGTTTT GTCTGCCACA
    GAAGAGTTGC CAAAGGATCA GACAGACAAG GATGTTGCTA AAATTGTATA TGTGTCTACT
    GTGCCAGAAC TGGTGTCACC AACTATTCCC GCATATACGT CAACTGACAC TACTAAGGAA
    AACCTACATG TGTTAACAGG GGGGGCAGTT GTTACATCAC TTGATAAAGA TGAGGAAGAA
    CATACACCCC ACACAAAAGA GAGCAAAATT ACACAGGTGA CAACAGGAGC AGAATTACCA
    GGTACCATAA CAGAGAGTAC TCATGGAATG TGGAGGGAAA CCCAAGAGCC AATAAATGTC
    ACACCTGATG CTGAAGACAA TCATTTGGGC AAAGAAGGGT ACATAATAAC AGAGATCCCA
    GAAGTTGCTG AAGTACCAGC TGGAGTGACA CCCAAAATGA TTGTGGAAAC TACAACAGCC
    ACTGAATCTG TGTTTACTGT GAAAGACACA TCTATAATTG TAGACAAAAC TGGCATTGTG
    AAACCTGTCG TGACTACGAC AGAGGCAGCT GAACTTACTG AGGTTGGATT CGAAGATTTA
    GGCACAGACA AAGTAACAAT TCTTGAAAAC ACACTTGAAG ATTCTGTAGG ACTAACAAAT
    GAACCTTTTG CTGTATCATG GGGTGTAACT GAAGCCACTA TTTTAGAGGA ATCATATGTG
    GAAAGTACTG AAAGTATTTC AGGTAAAGAG AAGTCCACTG AAACTATTAA GATCACAGGT
    GAAGCCACAA CTGATGACAT TGGTACTTCT GAAACTGACA TTGTAGTGCC TGTAACCGAT
    CGCATAGAAG ATAATGTAAA TGTATCACCT AAAAATGATG ATGCTAAGGA AACAAACATT
    TTTGAAGACA CAACACCAGA TCTTAGTGTT ACTAATTCTG AAAAAGACAT ACTGACAACT
    ACCATACATA AAGACATGCA TGAAGTTACA GAACCATTAA ATATTCCAGA GGTCACACAT
    AAGGTTGTGA TAGAGATCAC AGATGATGTA CGTGAGACCA GAGGAGATTC CTTACCCGAG
    ACACCAGAAG TCACAACAGA GACACCTAAG GTTGCAACAG AAGCTGATGT CAAAACAACA
    ACAAATGTTA TATCTAAAGC ACCTGAAGTT ATACTGAAGA CAACTGTAAC CAAGTCAGAT
    ACACCTGATG TCACAACTCA GACACCTTTG TTAAAGCTTG AAGATACAGA GAGGGTTGAA
    ATAGTACCTG AAACTCCTGA CATACAAGTG GTTCAAACAG AACACTCAGA AAATGATATC
    CCAACTCCTG TCTTGAAAGT GGAGGATTTC ACAGTTGCGC TGAATGACTT GACAACTCCT
    ATTCAAAAGA CTTCTTCAAC CACATTACAA CAAGACATTT CTAATGATAT TTTAGAAGAA
    AACAATGTGA TGGGAAATGA AATTGATGAT ATTGTGGTTC GACCAGTACG CCCTGTCGGC
    GATCATATTT TGGAGCTAAG TATTAAGCTA AGAGGGGAAA GCTATGACGA TGCTCTGAGG
    GATCCATCAA GTAACTACTA TCAACATCTG TCTACACTTT TTGTTGAAAA GATTGAAGAA
    GTTTTCGAAA GGCTTCCAGG TTTCAAGAAA ATATATGTTC TTGAATTCAG GCCACAGAAG
    GACATTGAAG GAGGTCTGGC AGTGGTGGTG CACTATGCTG TTGTTCTTGA GGGTGATGGG
    TCAGGAGTAA GTAATGAGAC CATGGATTAC ATAAACCTGC ATTCCAACAT GGTTGAGAAC
    TCCTTCACAG ATCCTGAGGA GCTGCCCACT GTGGTCTACA CCATCACTGA CTTCCGAAAC
    TACATCTCCG AAGCCCTTCA CAAGGATAAC TTTATCAGAA ACGCTACTCT GAATGTGGAC
    ATTGATTCTT TGCAACTTGA AAATGTGGAG ACATTGCTGC CTTCCAAACC AACCAGCAGA
    CCGTTTGACT CCAGTGACAT GATGGACAAC GTTCTTGCTT CCGAGAAACC ACCAGATGTT
    CCTGGGCAAG AATTATCCAA CAATGACATC TTCATTACAA ATTATGACAT CTTTGATCCT
    GATCCATGGA CAGGTGGACA AAGTGAAGAC AGAAATGCAA ATGATGTAAT CATCTTTGAG
    GAAAGTCCCA CTCAGTCTCC TGTTGAAGTG TCTTTGAAGA ACTTGGACAT TGAAGTTACT
    TCAAAATCTG AAACTTCAAG CAGTGCCCCT GTTCCTGATG AAGCTGTTAT TGAAGAGGAG
    GGATTTTTAC AGACTGCAAC TACAGTCAGT CCTATTATAG ACACAATAAC TGAATTTACT
    TTACCTACAG AATCACCTCA CAAGGTAGAA GAGACACCAG TTTCTCCTGT AGAACCTCCT
    GAGGTTGAGG AAACTACCCT GGGTGATTTA ACAGACCTTG GCTCAGGCTC AGGATTCTCT
    GGAGATCACA TAGGCCCTGA CATCTGGCCA TGGTCATCAG AAACATCACA AGAAGTCTTG
    AAGGAAGATA CCAATGATGA GATACACCAA CTTCAGGAAA ATCAGGAATC TATAATAAAA
    CAGCTTGAGC CTTTACATCA AGATGTGACT CCTGAGGAGC CATTCTTGGA TAGGGTTCTT
    GTGACCCAAG ACATTCGTAC CAATCCTCAT TACACAACAA CTGACCAGGC CCCTGTATTT
    TGGACAATGG AGACTTTGAC TGTTGAGCTT TCAATGCAGA CTCAAGTAGC CCCTGAACAT
    TATGATGACT ATTTTCCAGA TGCATCAATC ACTATGGCTA CACTTGTAAC CAATCAACCA
    TTGTTACATG TTCACAATCC ATTAGAAACA AAAGATCTGG ATGCATCACA AAGTCCAGGA
    ACAATAAGTT CAACTGCTGA TAAGGACTAT ACAGAACGAC TTGTTACACC CACTGTGATC
    CAACCAAGTG AAACAGTAGC TGTGGATAAG CATACTACAA CAGACAACAA ACAGAATCTG
    GAAACTATGG GTACATTATC TGCAACACTG GGCTCCACAA TGAAGTCCAC TGAAATCTCT
    TCAACTACCC AATTACCTGT GATCATCAAC AATAATACAG TTTCAGTGGA AGGTCCTGCC
    AATTCTTCAG AAGCTGTTCA GCATTCTGTA AATGAAGGAA TCACAGAGTT ACCTGCTTTG
    GTCTGGCCAG ATATTGAAGG ATCAGATGAA GAATTCAAAG TTTTAGATGA GGACATGGAA
    CTCGTCAAGT TTGTGCCTAC AAAAAGCCCA GTGACTGAAC TCTTAGAAGA AGATTTAGTT
    GGTGATGAAA TATTGGTTGC AACAACAACA ACAACAACAA CTCTATCCTC AACAACAGTA
    GCGACTGTGG TAACAAGTTT AGGTCATTCA GCCTCCCTGA CTCCTGAAAA GGACTCACCT
    TTCACTTGGA TATCACATTC ATCAATAGAT GAGGATGAAC CTGTGCAAGA GTCAACCACA
    GAACCTCTAC CCACCCAATC ACCCACTGCT TCTGTTCTTC CAAAGACAAC TACACAGACA
    TTGATGCACA CATACATAGC AGCTGTTGCA GGGGAGCCTG TTTTATCAGA TAACATGGAT
    AATAAGGACT CAACCACAAT GTCCACCCTT TTAGATGTTT CAAAACATTT AAAGACCCTT
    AGTTCCACCT CTGTACAATC ATTTTCAGAA CCAACATTTA CACCTACTGT CCACATAATT
    AAGCATGGAG AAACTTCAGA TGACTCAGTA CAAGATGTAG TTGAGACTGG AGAAGTTTTA
    GATGATTCTG TACAAGATGT ACTTGAGCAT GAGGACATTT TATCTGGCCC AGTTCAAGAT
    GTAGTTGTGT CTGGAGAAAC TGCAGAAGAC TCAGTACAAG ATTTAGTTGA GACTAGAGAA
    GTTTTAGATG ATTCTGTACA AGATGTACTT GAGCATGAGG ACATTTTATC TGGCCCAGTT
    CAAGATGTAG TTGTGTCTGG AGAAACTGCA GATGACTCAG TACAAGATTT AGTTGAGACT
    GGAGAAGTTT TAGATGATTC CGTACAAGAT GTACTTGAGC ATGAAGTAAT TTCATTTGGC
    CCAGTACAAG ATGTAGTTGT ATCTGGAAAA ACTTCAAATG ACTCAGTACA AGATGTAGTT
    GAGAGTGCAA AAGATTCAGA TGCCCCAATA CAAGATGTAT TTGAGCATAA AGACATTTCA
    GTTGACTCTG TACAAGATGT AGTTGTGTCT GGAGAAACTG CAGATGACTC TATTCAAGAT
    TTATTTAAGC ATGAAGGCAC TTCGTTCGAC ACAGTACAAG ATGAGGTTGA GTCTAGAGAA
    ACTTCAGATG ACTCAGTACA AAATGTAGTT GAGCATGAAG ACTCTTCAGA TGACGCAGTT
    CAAGATGTAG TCGAGTCTGG AGACACTTCA GATGACTCTG TACAAGATAT ATTTGAGTCT
    GCAGAAACTT CAGATGACTC TGTACAAGAT GTAGCTGAGA CTGGAAAAGA ATCAGATGAC
    TTAGTACAAG ATATATTTGA GAATAAAGAC ATTTCAGTTG ACTCAGTACA AGATGTAGTT
    GTATCTGGAG AGACTTCAGA TGACTCTATT CAAGGTTTAC TTGAGCATGA AGGCACTTCA
    GTCGACATAG TACAAGATGA GGTTGAGTTT AGAGAAAATT CAGATGACTC AGTACTAAAT
    GTAGTCAAGC ATGAAGATAC TTCAGATGAC TCAATACAAG ATGTAGTCGA GACAGGAGAA
    GTTTCAGATA GCTCAGTACA AGATGTAGCT GAGTCTGGAG AAACTTTAGA TGACTCTGTT
    CAAGATTTTG TTAAAACTGG AGAAATTTCC ATTGACTCAG TACACAATTT ATTTGAGCCT
    GAAAAAAACG TAGATGACTC TGTACAAGAT GTAGTTGAGA CTGGAGAAGT TTCTGTTGAT
    TCAGTACAAG ATGTAATTGA GCTTGGAGAC ACTTTAGATG ACTCTGCACA AGGAATAGTT
    GAACCTGAAG AAATGGCAGA CAACTTTATA AAGGAAAGCA CTGTAAAGCC AAGACCTGAC
    ATCAAAGGGC TTATTCTACC AACAGAGACA GTGAACTATG AAAGTGCCAC TATGAAAGTT
    CCTGCTCCAG TTAACCCCAG CATAACAGAA TTTAAGGTTT CATTTGACAT CTTCCCTTTC
    GACGACCCAA CTGATGATGA AGATAATGGC AGTGGGTTTG CTCATGGAAC TGACTTAGCA
    AGTTTAGCCT TGCCTGCCAG TCCTGGAAGG GCATTAATAG TGTTTTTTAG CCTTAGGGTC
    ACCAACATGA AGTTTTCCGA AGATCTCTTC AACAAGAGCT CTACTGAATA CAAGGCACTT
    GAGCAGCGAT TTCTGGAACT GCTCGTACCG TATCTACAGT CCAACCTCAG CAATTTCCAG
    AATTTAGAGA TTTTGAACTT CAGGAATGGT AGCATTGTGG TGAACAGCAG GATGAAGTTT
    GCCAAGCCAG TTCCCCATGG TGTGACAACA GCAGTGTACC TAATCTTGGA GGACTTCTGC
    AACACTGCCT ACCAAACTAT GAATCTTGCA ATAGACAAGT ATTCATTGGA TGTCGAATCA
    GGTGACCAGG CAGATCCTTG TAAGTTTCAG GCATGTAACG AGTTCTCCAA GTGTTTGGTG
    AACCGCTGGT CAGGTGAGGC CGAGTGTGTT TGTAACGCTG GTTATTTCAG CGTTGACGGA
    CTGCCATGCC AGAGCATCTG CGACATCCAA GAAGACTTCT GCCTAAATGA CGGGAAATGT
    GACATAATAC CTGGCAAGGG CGCCATCTGC AGGTGTCGCG TTGGTGAAAA CTGGTGGTAT
    CGAGGAGAAC ACTGTGAGGA ATATGTGTCA GAGCCGCTGG TGGTCGGCAT TGCCATTGCG
    TCCGTTGCTG GATTTCTCCT GGTGGCGTCG GGTGTCATCT TTTTCCTGGC CCGAACTCTG
    AGAGACCAGT ATGACACAGA TGATTCAGAA GACCCTTTAC GATATGCAGA GAACTTGCCA
    TCTCTAGAAA GAGCCACCAA GTACAACCCG ATGTTTGAAA GTGAAGTGAC TGCAGGCCAT
    AATCAGTATT ATCGGCGTTA CCCAGAAGCT CCGGCATTCA GCACGGGCAG CACCGAGATC
    TCCACTGACT TCAGCAGCGA AGAGATTCGT CACATCTATC AGAACAGTGA ACTTACTAAA
    GAGGAAATCC AGGACCGTAT TCGTATTATA GAGCTTTACG CCAAGGACCG CCAGTTTGCA
    GATTTTGTCC GACAGCACCA AGTGGCGCTT GACGCTAGAC GAGAGAGCTC TTCCAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329197.1
    CDS233..8572
    Translation

    Target ORF information:

    RefSeq Version XM_021473522.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio interphotoreceptor matrix proteoglycan 2b (impg2b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473522.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGCCTGATT TACCTTTTAA ATGCATTTGG TGGGGTTTGA CACTATTGTT GTTGACTGGA 
    TACTTTTCTA TAGATGCAGA TACTGGAACT CATGAGGATT ACTTGGCAAA TGGAAATACA
    ATGCTTTTGG ACACTAATGA AGTTTTGAAT CACTTATTGT TGTTGGACAA AGGTAAAACT
    TTGAATTACA TGTCTGCTGA AGGGAAAACA GCTCTATCTA GACATAAAAG GGACCTCCTG
    TTCCCCAATG GAGTTAAACT GTGCTCACAT GAGACGGTGC AACAGGCAGT GCAGAATCAT
    CTCAACTACT TTCACCTGCG AGTGTGCCAG GAGACAATTT GGGAGGCTTA TAAAATCTTC
    TGGGATCGCT TACCTGAGAA AGAAGAGTAC CAGATCTGGA TGAAAAAATG TCAAAATGGT
    AGCATTAGCG TGTTTGACAT CGGCCGAAGC TTTAGTCAGT CAGCTGAGCA CCTTGCTTTG
    ATTTCAAGTA GGGTAGCATC ATCCACAATA ACATCAACAA GTGCGCCCAC CAGCCCATGG
    CAACCTGAAT GCAGGCATCA GACGACGACT ACTTCGCCAA CACTAGCAAA AGCTGAACAA
    GTTGGTATTT TGATTCCTGA GGCAGTGCCA GGTTCACAAG TTGTCACACC GCAGACCTTT
    GCAGATGATA TCGTAATCAC AACTTTGCCT ACAAGGCTTC AAGTGACACC TGGAATTACC
    CCAAAGCCTG ATCTTGTCAC AGCCTTTAAG GACACAGTAA AACCTATTTC TGAAACTGAA
    CCTGAGGTCA TATTGGGTAC TACTTCTGAG AGTCCTGTTA AAGCATTGGA TACCATTACC
    ATTAAACATG AAGAGGTTGT TGTAGTCTCA GAATCTAAAT TATTGATTAC TGAGAGGACT
    TCGGAGGACT CTACTTCAAG GAAATATGAT TATGAGTTTC CCACGACCTT TAAGGACTCG
    TTAGTAATAA ACACTCCTAT AGTACTAACA ACTGAATTTT CTGAACAAAT GTATTCAGAA
    ATAGGACAAG GTTTTTCTGA TGGTACAGTT AAAACAGTAG CTGTTACTAT TCAAAAGGAA
    CTTGACATTG TCTCAAAATC TACATTATTA ACCACTGACA AAACTCCAGA AGTTTCTACT
    TCAAAAAAAT CTGAGCATGA TAATCCCACT AACTTTGTAG ATTCATTGGG AATAAGCACC
    AATACTCCTA GAGTGACCAC AAGTGAACTC AAAGAAGATC TTCATTCAGA ATTAGAACAG
    GTGGCTGGGC AGGATGTTAT TCACAGTCCT GTTAAAACAA CACATGTTAC AATTAAGCAT
    GAAGTGTTGG ACACAAATAC TACATTAATG ACAACTGACA GAACTCCATA TTCTACTTCA
    GCAAAATATG AACGTGTTAT ACCAACATCC TTTGAGGACT CACTAGTCAC AAGTTCATCT
    ACTGAACAGG ATATGACCAA TGTTCCTATT AAGACGGCAT ATGCCACAAT TTCACATGGA
    GAGTCTGAAA TTGTCACAGA GTCGACAATA ACAACCACTA ACAGAAATCT TGAAGATTCT
    TCTTTAGCAA AATATGATTA TGGGATTCCT CAAGGAATTG ATACTTCTTC GGGAGTAATT
    AATGAATCTT CTACAAAAGA ACATAATTCA GTATCTAAAT TGAATATTGG TCAGACTACT
    ACTGAAGGTC CCCAAACAAC TGATGCAGTT CAGTATGTAG AATCTGACAT TGTGTCAGAG
    GAAACGTTGA AATCCACTGG CAGAACTGCA GTTAGCTCAA ACTCTACAAA AATTGAGGCC
    GATCTTTCCA CAACTCCTGA GGACCTGACA GGAGTAAGTA CCAACACTCC TGAAATAGTC
    ACTAGAGATT CCATTACAAT ACTTATCCCA GAAACCGAGG GCATTACTAA AAATCCTGTT
    AAATCTGCAC CTATTACCAT TCAGCATGTG GATGTTGATG ATGTTTTAGC AGCTACAGTG
    ATGTCCACTC ATAAACCTCC CAAAGTCTCA CCTACAGAAA TATCCCCCGA TCAGAATCTC
    TTACCAGAGG AATCAAAGCT GCCAACTCCT ACAAAACTAG CAGAAGTTTT GTCTGCCACA
    GAAGAGTTGC CAAAGGATCA GACAGACAAG GATGTTGCTA AAATTGTATA TGTGTCTACT
    GTGCCAGAAC TGGTGTCACC AACTATTCCC GCATATACGT CAACTGACAC TACTAAGGAA
    AACCTACATG TGTTAACAGG GGGGGCAGTT GTTACATCAC TTGATAAAGA TGAGGAAGAA
    CATACACCCC ACACAAAAGA GAGCAAAATT ACACAGGTGA CAACAGGAGC AGAATTACCA
    GGTACCATAA CAGAGAGTAC TCATGGAATG TGGAGGGAAA CCCAAGAGCC AATAAATGTC
    ACACCTGATG CTGAAGACAA TCATTTGGGC AAAGAAGGGT ACATAATAAC AGAGATCCCA
    GAAGTTGCTG AAGTACCAGC TGGAGTGACA CCCAAAATGA TTGTGGAAAC TACAACAGCC
    ACTGAATCTG TGTTTACTGT GAAAGACACA TCTATAATTG TAGACAAAAC TGGCATTGTG
    AAACCTGTCG TGACTACGAC AGAGGCAGCT GAACTTACTG AGGTTGGATT CGAAGATTTA
    GGCACAGACA AAGTAACAAT TCTTGAAAAC ACACTTGAAG ATTCTGTAGG ACTAACAAAT
    GAACCTTTTG CTGTATCATG GGGTGTAACT GAAGCCACTA TTTTAGAGGA ATCATATGTG
    GAAAGTACTG AAAGTATTTC AGGTAAAGAG AAGTCCACTG AAACTATTAA GATCACAGGT
    GAAGCCACAA CTGATGACAT TGGTACTTCT GAAACTGACA TTGTAGTGCC TGTAACCGAT
    CGCATAGAAG ATAATGTAAA TGTATCACCT AAAAATGATG ATGCTAAGGA AACAAACATT
    TTTGAAGACA CAACACCAGA TCTTAGTGTT ACTAATTCTG AAAAAGACAT ACTGACAACT
    ACCATACATA AAGACATGCA TGAAGTTACA GAACCATTAA ATATTCCAGA GGTCACACAT
    AAGGTTGTGA TAGAGATCAC AGATGATGTA CGTGAGACCA GAGGAGATTC CTTACCCGAG
    ACACCAGAAG TCACAACAGA GACACCTAAG GTTGCAACAG AAGCTGATGT CAAAACAACA
    ACAAATGTTA TATCTAAAGC ACCTGAAGTT ATACTGAAGA CAACTGTAAC CAAGTCAGAT
    ACACCTGATG TCACAACTCA GACACCTTTG TTAAAGCTTG AAGATACAGA GAGGGTTGAA
    ATAGTACCTG AAACTCCTGA CATACAAGTG GTTCAAACAG AACACTCAGA AAATGATATC
    CCAACTCCTG TCTTGAAAGT GGAGGATTTC ACAGTTGCGC TGAATGACTT GACAACTCCT
    ATTCAAAAGA CTTCTTCAAC CACATTACAA CAAGACATTT CTAATGATAT TTTAGAAGAA
    AACAATGTGA TGGGAAATGA AATTGATGAT ATTGTGGTTC GACCAGTACG CCCTGTCGGC
    GATCATATTT TGGAGCTAAG TATTAAGCTA AGAGGGGAAA GCTATGACGA TGCTCTGAGG
    GATCCATCAA GTAACTACTA TCAACATCTG TCTACACTTT TTGTTGAAAA GATTGAAGAA
    GTTTTCGAAA GGCTTCCAGG TTTCAAGAAA ATATATGTTC TTGAATTCAG GCCACAGAAG
    GACATTGAAG GAGGTCTGGC AGTGGTGGTG CACTATGCTG TTGTTCTTGA GGGTGATGGG
    TCAGGAGTAA GTAATGAGAC CATGGATTAC ATAAACCTGC ATTCCAACAT GGTTGAGAAC
    TCCTTCACAG ATCCTGAGGA GCTGCCCACT GTGGTCTACA CCATCACTGA CTTCCGAAAC
    TACATCTCCG AAGCCCTTCA CAAGGATAAC TTTATCAGAA ACGCTACTCT GAATGTGGAC
    ATTGATTCTT TGCAACTTGA AAATGTGGAG ACATTGCTGC CTTCCAAACC AACCAGCAGA
    CCGTTTGACT CCAGTGACAT GATGGACAAC GTTCTTGCTT CCGAGAAACC ACCAGATGTT
    CCTGGGCAAG AATTATCCAA CAATGACATC TTCATTACAA ATTATGACAT CTTTGATCCT
    GATCCATGGA CAGGTGGACA AAGTGAAGAC AGAAATGCAA ATGATGTAAT CATCTTTGAG
    GAAAGTCCCA CTCAGTCTCC TGTTGAAGTG TCTTTGAAGA ACTTGGACAT TGAAGTTACT
    TCAAAATCTG AAACTTCAAG CAGTGCCCCT GTTCCTGATG AAGCTGTTAT TGAAGAGGAG
    GGATTTTTAC AGACTGCAAC TACAGTCAGT CCTATTATAG ACACAATAAC TGAATTTACT
    TTACCTACAG AATCACCTCA CAAGGTAGAA GAGACACCAG TTTCTCCTGT AGAACCTCCT
    GAGGTTGAGG AAACTACCCT GGGTGATTTA ACAGACCTTG GCTCAGGCTC AGGATTCTCT
    GGAGATCACA TAGGCCCTGA CATCTGGCCA TGGTCATCAG AAACATCACA AGAAGTCTTG
    AAGGAAGATA CCAATGATGA GATACACCAA CTTCAGGAAA ATCAGGAATC TATAATAAAA
    CAGCTTGAGC CTTTACATCA AGATGTGACT CCTGAGGAGC CATTCTTGGA TAGGGTTCTT
    GTGACCCAAG ACATTCGTAC CAATCCTCAT TACACAACAA CTGACCAGGC CCCTGTATTT
    TGGACAATGG AGACTTTGAC TGTTGAGCTT TCAATGCAGA CTCAAGTAGC CCCTGAACAT
    TATGATGACT ATTTTCCAGA TGCATCAATC ACTATGGCTA CACTTGTAAC CAATCAACCA
    TTGTTACATG TTCACAATCC ATTAGAAACA AAAGATCTGG ATGCATCACA AAGTCCAGGA
    ACAATAAGTT CAACTGCTGA TAAGGACTAT ACAGAACGAC TTGTTACACC CACTGTGATC
    CAACCAAGTG AAACAGTAGC TGTGGATAAG CATACTACAA CAGACAACAA ACAGAATCTG
    GAAACTATGG GTACATTATC TGCAACACTG GGCTCCACAA TGAAGTCCAC TGAAATCTCT
    TCAACTACCC AATTACCTGT GATCATCAAC AATAATACAG TTTCAGTGGA AGGTCCTGCC
    AATTCTTCAG AAGCTGTTCA GCATTCTGTA AATGAAGGAA TCACAGAGTT ACCTGCTTTG
    GTCTGGCCAG ATATTGAAGG ATCAGATGAA GAATTCAAAG TTTTAGATGA GGACATGGAA
    CTCGTCAAGT TTGTGCCTAC AAAAAGCCCA GTGACTGAAC TCTTAGAAGA AGATTTAGTT
    GGTGATGAAA TATTGGTTGC AACAACAACA ACAACAACAA CTCTATCCTC AACAACAGTA
    GCGACTGTGG TAACAAGTTT AGGTCATTCA GCCTCCCTGA CTCCTGAAAA GGACTCACCT
    TTCACTTGGA TATCACATTC ATCAATAGAT GAGGATGAAC CTGTGCAAGA GTCAACCACA
    GAACCTCTAC CCACCCAATC ACCCACTGCT TCTGTTCTTC CAAAGACAAC TACACAGACA
    TTGATGCACA CATACATAGC AGCTGTTGCA GGGGAGCCTG TTTTATCAGA TAACATGGAT
    AATAAGGACT CAACCACAAT GTCCACCCTT TTAGATGTTT CAAAACATTT AAAGACCCTT
    AGTTCCACCT CTGTACAATC ATTTTCAGAA CCAACATTTA CACCTACTGT CCACATAATT
    AAGCATGGAG AAACTTCAGA TGACTCAGTA CAAGATGTAG TTGAGACTGG AGAAGTTTTA
    GATGATTCTG TACAAGATGT ACTTGAGCAT GAGGACATTT TATCTGGCCC AGTTCAAGAT
    GTAGTTGTGT CTGGAGAAAC TGCAGAAGAC TCAGTACAAG ATTTAGTTGA GACTAGAGAA
    GTTTTAGATG ATTCTGTACA AGATGTACTT GAGCATGAGG ACATTTTATC TGGCCCAGTT
    CAAGATGTAG TTGTGTCTGG AGAAACTGCA GATGACTCAG TACAAGATTT AGTTGAGACT
    GGAGAAGTTT TAGATGATTC CGTACAAGAT GTACTTGAGC ATGAAGTAAT TTCATTTGGC
    CCAGTACAAG ATGTAGTTGT ATCTGGAAAA ACTTCAAATG ACTCAGTACA AGATGTAGTT
    GAGAGTGCAA AAGATTCAGA TGCCCCAATA CAAGATGTAT TTGAGCATAA AGACATTTCA
    GTTGACTCTG TACAAGATGT AGTTGTGTCT GGAGAAACTG CAGATGACTC TATTCAAGAT
    TTATTTAAGC ATGAAGGCAC TTCGTTCGAC ACAGTACAAG ATGAGGTTGA GTCTAGAGAA
    ACTTCAGATG ACTCAGTACA AAATGTAGTT GAGCATGAAG ACTCTTCAGA TGACGCAGTT
    CAAGATGTAG TCGAGTCTGG AGACACTTCA GATGACTCTG TACAAGATAT ATTTGAGTCT
    GCAGAAACTT CAGATGACTC TGTACAAGAT GTAGCTGAGA CTGGAAAAGA ATCAGATGAC
    TTAGTACAAG ATATATTTGA GAATAAAGAC ATTTCAGTTG ACTCAGTACA AGATGTAGTT
    GTATCTGGAG AGACTTCAGA TGACTCTATT CAAGGTTTAC TTGAGCATGA AGGCACTTCA
    GTCGACATAG TACAAGATGA GGTTGAGTTT AGAGAAAATT CAGATGACTC AGTACTAAAT
    GTAGTCAAGC ATGAAGATAC TTCAGATGAC TCAATACAAG ATGTAGTCGA GACAGGAGAA
    GTTTCAGATA GCTCAGTACA AGATGTAGCT GAGTCTGGAG AAACTTTAGA TGACTCTGTT
    CAAGATTTTG TTAAAACTGG AGAAATTTCC ATTGACTCAG TACACAATTT ATTTGAGCCT
    GAAAAAAACG TAGATGACTC TGTACAAGAT GTAGTTGAGA CTGGAGAAGT TTCTGTTGAT
    TCAGTACAAG ATGTAATTGA GCTTGGAGAC ACTTTAGATG ACTCTGCACA AGGAATAGTT
    GAACCTGAAG AAATGGCAGA CAACTTTATA AAGGAAAGCA CTGTAAAGCC AAGACCTGAC
    ATCAAAGGGC TTATTCTACC AACAGAGACA GTGAACTATG AAAGTGCCAC TATGAAAGTT
    CCTGCTCCAG TTAACCCCAG CATAACAGAA TTTAAGGTTT CATTTGACAT CTTCCCTTTC
    GACGACCCAA CTGATGATGA AGATAATGGC AGTGGGTTTG CTCATGGAAC TGACTTAGCA
    AGTTTAGCCT TGCCTGCCAG TCCTGGAAGG GCATTAATAG TGTTTTTTAG CCTTAGGGTC
    ACCAACATGA AGTTTTCCGA AGATCTCTTC AACAAGAGCT CTACTGAATA CAAGGCACTT
    GAGCAGCGAT TTCTGGAACT GCTCGTACCG TATCTACAGT CCAACCTCAG CAATTTCCAG
    AATTTAGAGA TTTTGAACTT CAGGAATGGT AGCATTGTGG TGAACAGCAG GATGAAGTTT
    GCCAAGCCAG TTCCCCATGG TGTGACAACA GCAGTGTACC TAATCTTGGA GGACTTCTGC
    AACACTGCCT ACCAAACTAT GAATCTTGCA ATAGACAAGT ATTCATTGGA TGTCGAATCA
    GGTGACCAGG CAGATCCTTG TAAGTTTCAG GCATGTAACG AGTTCTCCAA GTGTTTGGTG
    AACCGCTGGT CAGGTGAGGC CGAGTGTGTT TGTAACGCTG GTTATTTCAG CGTTGACGGA
    CTGCCATGCC AGAGCATCTG CGACATCCAA GAAGACTTCT GCCTAAATGA CGGGAAATGT
    GACATAATAC CTGGCAAGGG CGCCATCTGC AGGTGTCGCG TTGGTGAAAA CTGGTGGTAT
    CGAGGAGAAC ACTGTGAGGA ATATGTGTCA GAGCCGCTGG TGGTCGGCAT TGCCATTGCG
    TCCGTTGCTG GATTTCTCCT GGTGGCGTCG GGTGTCATCT TTTTCCTGGC CCGAACTCTG
    AGAGACCAGT ATGACACAGA TGATTCAGAA GACCCTTTAC GATATGCAGA GAACTTGCCA
    TCTCTAGAAA GAGCCACCAA GTACAACCCG ATGTTTGAAA GTGAAGTGAC TGCAGGCCAT
    AATCAGTATT ATCGGCGTTA CCCAGAAGCT CCGGCATTCA GCACGGGCAG CACCGAGATC
    TCCACTGACT TCAGCAGCGA AGAGATTCGT CACATCTATC AGAACAGTGA ACTTACTAAA
    GAGGAAATCC AGGACCGTAT TCGTATTATA GAGCTTTACG CCAAGGACCG CCAGTTTGCA
    GATTTTGTCC GACAGCACCA AGTGGCGCTT GACGCTAGAC GAGAGAGCTC TTCCAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146186
    Clone ID Related Accession (Same CDS sequence) XM_021473522.1 , XM_021469612.1 , XM_021474486.1
    Accession Version XM_021469612.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8340bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin homolog isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
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    3481
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    4141
    4201
    4261
    4321
    4381
    4441
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    4621
    4681
    4741
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    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
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    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
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    6961
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    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
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    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGCCTGATT TACCTTTTAA ATGCATTTGG TGGGGTTTGA CACTATTGTT GTTGACTGGA 
    TACTTTTCTA TAGATGCAGA TACTGGAACT CATGAGGATT ACTTGGCAAA TGGAAATACA
    ATGCTTTTGG ACACTAATGA AGTTTTGAAT CACTTATTGT TGTTGGACAA AGGTAAAACT
    TTGAATTACA TGTCTGCTGA AGGGAAAACA GCTCTATCTA GACATAAAAG GGACCTCCTG
    TTCCCCAATG GAGTTAAACT GTGCTCACAT GAGACGGTGC AACAGGCAGT GCAGAATCAT
    CTCAACTACT TTCACCTGCG AGTGTGCCAG GAGACAATTT GGGAGGCTTA TAAAATCTTC
    TGGGATCGCT TACCTGAGAA AGAAGAGTAC CAGATCTGGA TGAAAAAATG TCAAAATGGT
    AGCATTAGCG TGTTTGACAT CGGCCGAAGC TTTAGTCAGT CAGCTGAGCA CCTTGCTTTG
    ATTTCAAGTA GGGTAGCATC ATCCACAATA ACATCAACAA GTGCGCCCAC CAGCCCATGG
    CAACCTGAAT GCAGGCATCA GACGACGACT ACTTCGCCAA CACTAGCAAA AGCTGAACAA
    GTTGGTATTT TGATTCCTGA GGCAGTGCCA GGTTCACAAG TTGTCACACC GCAGACCTTT
    GCAGATGATA TCGTAATCAC AACTTTGCCT ACAAGGCTTC AAGTGACACC TGGAATTACC
    CCAAAGCCTG ATCTTGTCAC AGCCTTTAAG GACACAGTAA AACCTATTTC TGAAACTGAA
    CCTGAGGTCA TATTGGGTAC TACTTCTGAG AGTCCTGTTA AAGCATTGGA TACCATTACC
    ATTAAACATG AAGAGGTTGT TGTAGTCTCA GAATCTAAAT TATTGATTAC TGAGAGGACT
    TCGGAGGACT CTACTTCAAG GAAATATGAT TATGAGTTTC CCACGACCTT TAAGGACTCG
    TTAGTAATAA ACACTCCTAT AGTACTAACA ACTGAATTTT CTGAACAAAT GTATTCAGAA
    ATAGGACAAG GTTTTTCTGA TGGTACAGTT AAAACAGTAG CTGTTACTAT TCAAAAGGAA
    CTTGACATTG TCTCAAAATC TACATTATTA ACCACTGACA AAACTCCAGA AGTTTCTACT
    TCAAAAAAAT CTGAGCATGA TAATCCCACT AACTTTGTAG ATTCATTGGG AATAAGCACC
    AATACTCCTA GAGTGACCAC AAGTGAACTC AAAGAAGATC TTCATTCAGA ATTAGAACAG
    GTGGCTGGGC AGGATGTTAT TCACAGTCCT GTTAAAACAA CACATGTTAC AATTAAGCAT
    GAAGTGTTGG ACACAAATAC TACATTAATG ACAACTGACA GAACTCCATA TTCTACTTCA
    GCAAAATATG AACGTGTTAT ACCAACATCC TTTGAGGACT CACTAGTCAC AAGTTCATCT
    ACTGAACAGG ATATGACCAA TGTTCCTATT AAGACGGCAT ATGCCACAAT TTCACATGGA
    GAGTCTGAAA TTGTCACAGA GTCGACAATA ACAACCACTA ACAGAAATCT TGAAGATTCT
    TCTTTAGCAA AATATGATTA TGGGATTCCT CAAGGAATTG ATACTTCTTC GGGAGTAATT
    AATGAATCTT CTACAAAAGA ACATAATTCA GTATCTAAAT TGAATATTGG TCAGACTACT
    ACTGAAGGTC CCCAAACAAC TGATGCAGTT CAGTATGTAG AATCTGACAT TGTGTCAGAG
    GAAACGTTGA AATCCACTGG CAGAACTGCA GTTAGCTCAA ACTCTACAAA AATTGAGGCC
    GATCTTTCCA CAACTCCTGA GGACCTGACA GGAGTAAGTA CCAACACTCC TGAAATAGTC
    ACTAGAGATT CCATTACAAT ACTTATCCCA GAAACCGAGG GCATTACTAA AAATCCTGTT
    AAATCTGCAC CTATTACCAT TCAGCATGTG GATGTTGATG ATGTTTTAGC AGCTACAGTG
    ATGTCCACTC ATAAACCTCC CAAAGTCTCA CCTACAGAAA TATCCCCCGA TCAGAATCTC
    TTACCAGAGG AATCAAAGCT GCCAACTCCT ACAAAACTAG CAGAAGTTTT GTCTGCCACA
    GAAGAGTTGC CAAAGGATCA GACAGACAAG GATGTTGCTA AAATTGTATA TGTGTCTACT
    GTGCCAGAAC TGGTGTCACC AACTATTCCC GCATATACGT CAACTGACAC TACTAAGGAA
    AACCTACATG TGTTAACAGG GGGGGCAGTT GTTACATCAC TTGATAAAGA TGAGGAAGAA
    CATACACCCC ACACAAAAGA GAGCAAAATT ACACAGGTGA CAACAGGAGC AGAATTACCA
    GGTACCATAA CAGAGAGTAC TCATGGAATG TGGAGGGAAA CCCAAGAGCC AATAAATGTC
    ACACCTGATG CTGAAGACAA TCATTTGGGC AAAGAAGGGT ACATAATAAC AGAGATCCCA
    GAAGTTGCTG AAGTACCAGC TGGAGTGACA CCCAAAATGA TTGTGGAAAC TACAACAGCC
    ACTGAATCTG TGTTTACTGT GAAAGACACA TCTATAATTG TAGACAAAAC TGGCATTGTG
    AAACCTGTCG TGACTACGAC AGAGGCAGCT GAACTTACTG AGGTTGGATT CGAAGATTTA
    GGCACAGACA AAGTAACAAT TCTTGAAAAC ACACTTGAAG ATTCTGTAGG ACTAACAAAT
    GAACCTTTTG CTGTATCATG GGGTGTAACT GAAGCCACTA TTTTAGAGGA ATCATATGTG
    GAAAGTACTG AAAGTATTTC AGGTAAAGAG AAGTCCACTG AAACTATTAA GATCACAGGT
    GAAGCCACAA CTGATGACAT TGGTACTTCT GAAACTGACA TTGTAGTGCC TGTAACCGAT
    CGCATAGAAG ATAATGTAAA TGTATCACCT AAAAATGATG ATGCTAAGGA AACAAACATT
    TTTGAAGACA CAACACCAGA TCTTAGTGTT ACTAATTCTG AAAAAGACAT ACTGACAACT
    ACCATACATA AAGACATGCA TGAAGTTACA GAACCATTAA ATATTCCAGA GGTCACACAT
    AAGGTTGTGA TAGAGATCAC AGATGATGTA CGTGAGACCA GAGGAGATTC CTTACCCGAG
    ACACCAGAAG TCACAACAGA GACACCTAAG GTTGCAACAG AAGCTGATGT CAAAACAACA
    ACAAATGTTA TATCTAAAGC ACCTGAAGTT ATACTGAAGA CAACTGTAAC CAAGTCAGAT
    ACACCTGATG TCACAACTCA GACACCTTTG TTAAAGCTTG AAGATACAGA GAGGGTTGAA
    ATAGTACCTG AAACTCCTGA CATACAAGTG GTTCAAACAG AACACTCAGA AAATGATATC
    CCAACTCCTG TCTTGAAAGT GGAGGATTTC ACAGTTGCGC TGAATGACTT GACAACTCCT
    ATTCAAAAGA CTTCTTCAAC CACATTACAA CAAGACATTT CTAATGATAT TTTAGAAGAA
    AACAATGTGA TGGGAAATGA AATTGATGAT ATTGTGGTTC GACCAGTACG CCCTGTCGGC
    GATCATATTT TGGAGCTAAG TATTAAGCTA AGAGGGGAAA GCTATGACGA TGCTCTGAGG
    GATCCATCAA GTAACTACTA TCAACATCTG TCTACACTTT TTGTTGAAAA GATTGAAGAA
    GTTTTCGAAA GGCTTCCAGG TTTCAAGAAA ATATATGTTC TTGAATTCAG GCCACAGAAG
    GACATTGAAG GAGGTCTGGC AGTGGTGGTG CACTATGCTG TTGTTCTTGA GGGTGATGGG
    TCAGGAGTAA GTAATGAGAC CATGGATTAC ATAAACCTGC ATTCCAACAT GGTTGAGAAC
    TCCTTCACAG ATCCTGAGGA GCTGCCCACT GTGGTCTACA CCATCACTGA CTTCCGAAAC
    TACATCTCCG AAGCCCTTCA CAAGGATAAC TTTATCAGAA ACGCTACTCT GAATGTGGAC
    ATTGATTCTT TGCAACTTGA AAATGTGGAG ACATTGCTGC CTTCCAAACC AACCAGCAGA
    CCGTTTGACT CCAGTGACAT GATGGACAAC GTTCTTGCTT CCGAGAAACC ACCAGATGTT
    CCTGGGCAAG AATTATCCAA CAATGACATC TTCATTACAA ATTATGACAT CTTTGATCCT
    GATCCATGGA CAGGTGGACA AAGTGAAGAC AGAAATGCAA ATGATGTAAT CATCTTTGAG
    GAAAGTCCCA CTCAGTCTCC TGTTGAAGTG TCTTTGAAGA ACTTGGACAT TGAAGTTACT
    TCAAAATCTG AAACTTCAAG CAGTGCCCCT GTTCCTGATG AAGCTGTTAT TGAAGAGGAG
    GGATTTTTAC AGACTGCAAC TACAGTCAGT CCTATTATAG ACACAATAAC TGAATTTACT
    TTACCTACAG AATCACCTCA CAAGGTAGAA GAGACACCAG TTTCTCCTGT AGAACCTCCT
    GAGGTTGAGG AAACTACCCT GGGTGATTTA ACAGACCTTG GCTCAGGCTC AGGATTCTCT
    GGAGATCACA TAGGCCCTGA CATCTGGCCA TGGTCATCAG AAACATCACA AGAAGTCTTG
    AAGGAAGATA CCAATGATGA GATACACCAA CTTCAGGAAA ATCAGGAATC TATAATAAAA
    CAGCTTGAGC CTTTACATCA AGATGTGACT CCTGAGGAGC CATTCTTGGA TAGGGTTCTT
    GTGACCCAAG ACATTCGTAC CAATCCTCAT TACACAACAA CTGACCAGGC CCCTGTATTT
    TGGACAATGG AGACTTTGAC TGTTGAGCTT TCAATGCAGA CTCAAGTAGC CCCTGAACAT
    TATGATGACT ATTTTCCAGA TGCATCAATC ACTATGGCTA CACTTGTAAC CAATCAACCA
    TTGTTACATG TTCACAATCC ATTAGAAACA AAAGATCTGG ATGCATCACA AAGTCCAGGA
    ACAATAAGTT CAACTGCTGA TAAGGACTAT ACAGAACGAC TTGTTACACC CACTGTGATC
    CAACCAAGTG AAACAGTAGC TGTGGATAAG CATACTACAA CAGACAACAA ACAGAATCTG
    GAAACTATGG GTACATTATC TGCAACACTG GGCTCCACAA TGAAGTCCAC TGAAATCTCT
    TCAACTACCC AATTACCTGT GATCATCAAC AATAATACAG TTTCAGTGGA AGGTCCTGCC
    AATTCTTCAG AAGCTGTTCA GCATTCTGTA AATGAAGGAA TCACAGAGTT ACCTGCTTTG
    GTCTGGCCAG ATATTGAAGG ATCAGATGAA GAATTCAAAG TTTTAGATGA GGACATGGAA
    CTCGTCAAGT TTGTGCCTAC AAAAAGCCCA GTGACTGAAC TCTTAGAAGA AGATTTAGTT
    GGTGATGAAA TATTGGTTGC AACAACAACA ACAACAACAA CTCTATCCTC AACAACAGTA
    GCGACTGTGG TAACAAGTTT AGGTCATTCA GCCTCCCTGA CTCCTGAAAA GGACTCACCT
    TTCACTTGGA TATCACATTC ATCAATAGAT GAGGATGAAC CTGTGCAAGA GTCAACCACA
    GAACCTCTAC CCACCCAATC ACCCACTGCT TCTGTTCTTC CAAAGACAAC TACACAGACA
    TTGATGCACA CATACATAGC AGCTGTTGCA GGGGAGCCTG TTTTATCAGA TAACATGGAT
    AATAAGGACT CAACCACAAT GTCCACCCTT TTAGATGTTT CAAAACATTT AAAGACCCTT
    AGTTCCACCT CTGTACAATC ATTTTCAGAA CCAACATTTA CACCTACTGT CCACATAATT
    AAGCATGGAG AAACTTCAGA TGACTCAGTA CAAGATGTAG TTGAGACTGG AGAAGTTTTA
    GATGATTCTG TACAAGATGT ACTTGAGCAT GAGGACATTT TATCTGGCCC AGTTCAAGAT
    GTAGTTGTGT CTGGAGAAAC TGCAGAAGAC TCAGTACAAG ATTTAGTTGA GACTAGAGAA
    GTTTTAGATG ATTCTGTACA AGATGTACTT GAGCATGAGG ACATTTTATC TGGCCCAGTT
    CAAGATGTAG TTGTGTCTGG AGAAACTGCA GATGACTCAG TACAAGATTT AGTTGAGACT
    GGAGAAGTTT TAGATGATTC CGTACAAGAT GTACTTGAGC ATGAAGTAAT TTCATTTGGC
    CCAGTACAAG ATGTAGTTGT ATCTGGAAAA ACTTCAAATG ACTCAGTACA AGATGTAGTT
    GAGAGTGCAA AAGATTCAGA TGCCCCAATA CAAGATGTAT TTGAGCATAA AGACATTTCA
    GTTGACTCTG TACAAGATGT AGTTGTGTCT GGAGAAACTG CAGATGACTC TATTCAAGAT
    TTATTTAAGC ATGAAGGCAC TTCGTTCGAC ACAGTACAAG ATGAGGTTGA GTCTAGAGAA
    ACTTCAGATG ACTCAGTACA AAATGTAGTT GAGCATGAAG ACTCTTCAGA TGACGCAGTT
    CAAGATGTAG TCGAGTCTGG AGACACTTCA GATGACTCTG TACAAGATAT ATTTGAGTCT
    GCAGAAACTT CAGATGACTC TGTACAAGAT GTAGCTGAGA CTGGAAAAGA ATCAGATGAC
    TTAGTACAAG ATATATTTGA GAATAAAGAC ATTTCAGTTG ACTCAGTACA AGATGTAGTT
    GTATCTGGAG AGACTTCAGA TGACTCTATT CAAGGTTTAC TTGAGCATGA AGGCACTTCA
    GTCGACATAG TACAAGATGA GGTTGAGTTT AGAGAAAATT CAGATGACTC AGTACTAAAT
    GTAGTCAAGC ATGAAGATAC TTCAGATGAC TCAATACAAG ATGTAGTCGA GACAGGAGAA
    GTTTCAGATA GCTCAGTACA AGATGTAGCT GAGTCTGGAG AAACTTTAGA TGACTCTGTT
    CAAGATTTTG TTAAAACTGG AGAAATTTCC ATTGACTCAG TACACAATTT ATTTGAGCCT
    GAAAAAAACG TAGATGACTC TGTACAAGAT GTAGTTGAGA CTGGAGAAGT TTCTGTTGAT
    TCAGTACAAG ATGTAATTGA GCTTGGAGAC ACTTTAGATG ACTCTGCACA AGGAATAGTT
    GAACCTGAAG AAATGGCAGA CAACTTTATA AAGGAAAGCA CTGTAAAGCC AAGACCTGAC
    ATCAAAGGGC TTATTCTACC AACAGAGACA GTGAACTATG AAAGTGCCAC TATGAAAGTT
    CCTGCTCCAG TTAACCCCAG CATAACAGAA TTTAAGGTTT CATTTGACAT CTTCCCTTTC
    GACGACCCAA CTGATGATGA AGATAATGGC AGTGGGTTTG CTCATGGAAC TGACTTAGCA
    AGTTTAGCCT TGCCTGCCAG TCCTGGAAGG GCATTAATAG TGTTTTTTAG CCTTAGGGTC
    ACCAACATGA AGTTTTCCGA AGATCTCTTC AACAAGAGCT CTACTGAATA CAAGGCACTT
    GAGCAGCGAT TTCTGGAACT GCTCGTACCG TATCTACAGT CCAACCTCAG CAATTTCCAG
    AATTTAGAGA TTTTGAACTT CAGGAATGGT AGCATTGTGG TGAACAGCAG GATGAAGTTT
    GCCAAGCCAG TTCCCCATGG TGTGACAACA GCAGTGTACC TAATCTTGGA GGACTTCTGC
    AACACTGCCT ACCAAACTAT GAATCTTGCA ATAGACAAGT ATTCATTGGA TGTCGAATCA
    GGTGACCAGG CAGATCCTTG TAAGTTTCAG GCATGTAACG AGTTCTCCAA GTGTTTGGTG
    AACCGCTGGT CAGGTGAGGC CGAGTGTGTT TGTAACGCTG GTTATTTCAG CGTTGACGGA
    CTGCCATGCC AGAGCATCTG CGACATCCAA GAAGACTTCT GCCTAAATGA CGGGAAATGT
    GACATAATAC CTGGCAAGGG CGCCATCTGC AGGTGTCGCG TTGGTGAAAA CTGGTGGTAT
    CGAGGAGAAC ACTGTGAGGA ATATGTGTCA GAGCCGCTGG TGGTCGGCAT TGCCATTGCG
    TCCGTTGCTG GATTTCTCCT GGTGGCGTCG GGTGTCATCT TTTTCCTGGC CCGAACTCTG
    AGAGACCAGT ATGACACAGA TGATTCAGAA GACCCTTTAC GATATGCAGA GAACTTGCCA
    TCTCTAGAAA GAGCCACCAA GTACAACCCG ATGTTTGAAA GTGAAGTGAC TGCAGGCCAT
    AATCAGTATT ATCGGCGTTA CCCAGAAGCT CCGGCATTCA GCACGGGCAG CACCGAGATC
    TCCACTGACT TCAGCAGCGA AGAGATTCGT CACATCTATC AGAACAGTGA ACTTACTAAA
    GAGGAAATCC AGGACCGTAT TCGTATTATA GAGCTTTACG CCAAGGACCG CCAGTTTGCA
    GATTTTGTCC GACAGCACCA AGTGGCGCTT GACGCTAGAC GAGAGAGCTC TTCCAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325287.1
    CDS233..8572
    Translation

    Target ORF information:

    RefSeq Version XM_021469612.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio interphotoreceptor matrix proteoglycan 2b (impg2b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469612.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGCCTGATT TACCTTTTAA ATGCATTTGG TGGGGTTTGA CACTATTGTT GTTGACTGGA 
    TACTTTTCTA TAGATGCAGA TACTGGAACT CATGAGGATT ACTTGGCAAA TGGAAATACA
    ATGCTTTTGG ACACTAATGA AGTTTTGAAT CACTTATTGT TGTTGGACAA AGGTAAAACT
    TTGAATTACA TGTCTGCTGA AGGGAAAACA GCTCTATCTA GACATAAAAG GGACCTCCTG
    TTCCCCAATG GAGTTAAACT GTGCTCACAT GAGACGGTGC AACAGGCAGT GCAGAATCAT
    CTCAACTACT TTCACCTGCG AGTGTGCCAG GAGACAATTT GGGAGGCTTA TAAAATCTTC
    TGGGATCGCT TACCTGAGAA AGAAGAGTAC CAGATCTGGA TGAAAAAATG TCAAAATGGT
    AGCATTAGCG TGTTTGACAT CGGCCGAAGC TTTAGTCAGT CAGCTGAGCA CCTTGCTTTG
    ATTTCAAGTA GGGTAGCATC ATCCACAATA ACATCAACAA GTGCGCCCAC CAGCCCATGG
    CAACCTGAAT GCAGGCATCA GACGACGACT ACTTCGCCAA CACTAGCAAA AGCTGAACAA
    GTTGGTATTT TGATTCCTGA GGCAGTGCCA GGTTCACAAG TTGTCACACC GCAGACCTTT
    GCAGATGATA TCGTAATCAC AACTTTGCCT ACAAGGCTTC AAGTGACACC TGGAATTACC
    CCAAAGCCTG ATCTTGTCAC AGCCTTTAAG GACACAGTAA AACCTATTTC TGAAACTGAA
    CCTGAGGTCA TATTGGGTAC TACTTCTGAG AGTCCTGTTA AAGCATTGGA TACCATTACC
    ATTAAACATG AAGAGGTTGT TGTAGTCTCA GAATCTAAAT TATTGATTAC TGAGAGGACT
    TCGGAGGACT CTACTTCAAG GAAATATGAT TATGAGTTTC CCACGACCTT TAAGGACTCG
    TTAGTAATAA ACACTCCTAT AGTACTAACA ACTGAATTTT CTGAACAAAT GTATTCAGAA
    ATAGGACAAG GTTTTTCTGA TGGTACAGTT AAAACAGTAG CTGTTACTAT TCAAAAGGAA
    CTTGACATTG TCTCAAAATC TACATTATTA ACCACTGACA AAACTCCAGA AGTTTCTACT
    TCAAAAAAAT CTGAGCATGA TAATCCCACT AACTTTGTAG ATTCATTGGG AATAAGCACC
    AATACTCCTA GAGTGACCAC AAGTGAACTC AAAGAAGATC TTCATTCAGA ATTAGAACAG
    GTGGCTGGGC AGGATGTTAT TCACAGTCCT GTTAAAACAA CACATGTTAC AATTAAGCAT
    GAAGTGTTGG ACACAAATAC TACATTAATG ACAACTGACA GAACTCCATA TTCTACTTCA
    GCAAAATATG AACGTGTTAT ACCAACATCC TTTGAGGACT CACTAGTCAC AAGTTCATCT
    ACTGAACAGG ATATGACCAA TGTTCCTATT AAGACGGCAT ATGCCACAAT TTCACATGGA
    GAGTCTGAAA TTGTCACAGA GTCGACAATA ACAACCACTA ACAGAAATCT TGAAGATTCT
    TCTTTAGCAA AATATGATTA TGGGATTCCT CAAGGAATTG ATACTTCTTC GGGAGTAATT
    AATGAATCTT CTACAAAAGA ACATAATTCA GTATCTAAAT TGAATATTGG TCAGACTACT
    ACTGAAGGTC CCCAAACAAC TGATGCAGTT CAGTATGTAG AATCTGACAT TGTGTCAGAG
    GAAACGTTGA AATCCACTGG CAGAACTGCA GTTAGCTCAA ACTCTACAAA AATTGAGGCC
    GATCTTTCCA CAACTCCTGA GGACCTGACA GGAGTAAGTA CCAACACTCC TGAAATAGTC
    ACTAGAGATT CCATTACAAT ACTTATCCCA GAAACCGAGG GCATTACTAA AAATCCTGTT
    AAATCTGCAC CTATTACCAT TCAGCATGTG GATGTTGATG ATGTTTTAGC AGCTACAGTG
    ATGTCCACTC ATAAACCTCC CAAAGTCTCA CCTACAGAAA TATCCCCCGA TCAGAATCTC
    TTACCAGAGG AATCAAAGCT GCCAACTCCT ACAAAACTAG CAGAAGTTTT GTCTGCCACA
    GAAGAGTTGC CAAAGGATCA GACAGACAAG GATGTTGCTA AAATTGTATA TGTGTCTACT
    GTGCCAGAAC TGGTGTCACC AACTATTCCC GCATATACGT CAACTGACAC TACTAAGGAA
    AACCTACATG TGTTAACAGG GGGGGCAGTT GTTACATCAC TTGATAAAGA TGAGGAAGAA
    CATACACCCC ACACAAAAGA GAGCAAAATT ACACAGGTGA CAACAGGAGC AGAATTACCA
    GGTACCATAA CAGAGAGTAC TCATGGAATG TGGAGGGAAA CCCAAGAGCC AATAAATGTC
    ACACCTGATG CTGAAGACAA TCATTTGGGC AAAGAAGGGT ACATAATAAC AGAGATCCCA
    GAAGTTGCTG AAGTACCAGC TGGAGTGACA CCCAAAATGA TTGTGGAAAC TACAACAGCC
    ACTGAATCTG TGTTTACTGT GAAAGACACA TCTATAATTG TAGACAAAAC TGGCATTGTG
    AAACCTGTCG TGACTACGAC AGAGGCAGCT GAACTTACTG AGGTTGGATT CGAAGATTTA
    GGCACAGACA AAGTAACAAT TCTTGAAAAC ACACTTGAAG ATTCTGTAGG ACTAACAAAT
    GAACCTTTTG CTGTATCATG GGGTGTAACT GAAGCCACTA TTTTAGAGGA ATCATATGTG
    GAAAGTACTG AAAGTATTTC AGGTAAAGAG AAGTCCACTG AAACTATTAA GATCACAGGT
    GAAGCCACAA CTGATGACAT TGGTACTTCT GAAACTGACA TTGTAGTGCC TGTAACCGAT
    CGCATAGAAG ATAATGTAAA TGTATCACCT AAAAATGATG ATGCTAAGGA AACAAACATT
    TTTGAAGACA CAACACCAGA TCTTAGTGTT ACTAATTCTG AAAAAGACAT ACTGACAACT
    ACCATACATA AAGACATGCA TGAAGTTACA GAACCATTAA ATATTCCAGA GGTCACACAT
    AAGGTTGTGA TAGAGATCAC AGATGATGTA CGTGAGACCA GAGGAGATTC CTTACCCGAG
    ACACCAGAAG TCACAACAGA GACACCTAAG GTTGCAACAG AAGCTGATGT CAAAACAACA
    ACAAATGTTA TATCTAAAGC ACCTGAAGTT ATACTGAAGA CAACTGTAAC CAAGTCAGAT
    ACACCTGATG TCACAACTCA GACACCTTTG TTAAAGCTTG AAGATACAGA GAGGGTTGAA
    ATAGTACCTG AAACTCCTGA CATACAAGTG GTTCAAACAG AACACTCAGA AAATGATATC
    CCAACTCCTG TCTTGAAAGT GGAGGATTTC ACAGTTGCGC TGAATGACTT GACAACTCCT
    ATTCAAAAGA CTTCTTCAAC CACATTACAA CAAGACATTT CTAATGATAT TTTAGAAGAA
    AACAATGTGA TGGGAAATGA AATTGATGAT ATTGTGGTTC GACCAGTACG CCCTGTCGGC
    GATCATATTT TGGAGCTAAG TATTAAGCTA AGAGGGGAAA GCTATGACGA TGCTCTGAGG
    GATCCATCAA GTAACTACTA TCAACATCTG TCTACACTTT TTGTTGAAAA GATTGAAGAA
    GTTTTCGAAA GGCTTCCAGG TTTCAAGAAA ATATATGTTC TTGAATTCAG GCCACAGAAG
    GACATTGAAG GAGGTCTGGC AGTGGTGGTG CACTATGCTG TTGTTCTTGA GGGTGATGGG
    TCAGGAGTAA GTAATGAGAC CATGGATTAC ATAAACCTGC ATTCCAACAT GGTTGAGAAC
    TCCTTCACAG ATCCTGAGGA GCTGCCCACT GTGGTCTACA CCATCACTGA CTTCCGAAAC
    TACATCTCCG AAGCCCTTCA CAAGGATAAC TTTATCAGAA ACGCTACTCT GAATGTGGAC
    ATTGATTCTT TGCAACTTGA AAATGTGGAG ACATTGCTGC CTTCCAAACC AACCAGCAGA
    CCGTTTGACT CCAGTGACAT GATGGACAAC GTTCTTGCTT CCGAGAAACC ACCAGATGTT
    CCTGGGCAAG AATTATCCAA CAATGACATC TTCATTACAA ATTATGACAT CTTTGATCCT
    GATCCATGGA CAGGTGGACA AAGTGAAGAC AGAAATGCAA ATGATGTAAT CATCTTTGAG
    GAAAGTCCCA CTCAGTCTCC TGTTGAAGTG TCTTTGAAGA ACTTGGACAT TGAAGTTACT
    TCAAAATCTG AAACTTCAAG CAGTGCCCCT GTTCCTGATG AAGCTGTTAT TGAAGAGGAG
    GGATTTTTAC AGACTGCAAC TACAGTCAGT CCTATTATAG ACACAATAAC TGAATTTACT
    TTACCTACAG AATCACCTCA CAAGGTAGAA GAGACACCAG TTTCTCCTGT AGAACCTCCT
    GAGGTTGAGG AAACTACCCT GGGTGATTTA ACAGACCTTG GCTCAGGCTC AGGATTCTCT
    GGAGATCACA TAGGCCCTGA CATCTGGCCA TGGTCATCAG AAACATCACA AGAAGTCTTG
    AAGGAAGATA CCAATGATGA GATACACCAA CTTCAGGAAA ATCAGGAATC TATAATAAAA
    CAGCTTGAGC CTTTACATCA AGATGTGACT CCTGAGGAGC CATTCTTGGA TAGGGTTCTT
    GTGACCCAAG ACATTCGTAC CAATCCTCAT TACACAACAA CTGACCAGGC CCCTGTATTT
    TGGACAATGG AGACTTTGAC TGTTGAGCTT TCAATGCAGA CTCAAGTAGC CCCTGAACAT
    TATGATGACT ATTTTCCAGA TGCATCAATC ACTATGGCTA CACTTGTAAC CAATCAACCA
    TTGTTACATG TTCACAATCC ATTAGAAACA AAAGATCTGG ATGCATCACA AAGTCCAGGA
    ACAATAAGTT CAACTGCTGA TAAGGACTAT ACAGAACGAC TTGTTACACC CACTGTGATC
    CAACCAAGTG AAACAGTAGC TGTGGATAAG CATACTACAA CAGACAACAA ACAGAATCTG
    GAAACTATGG GTACATTATC TGCAACACTG GGCTCCACAA TGAAGTCCAC TGAAATCTCT
    TCAACTACCC AATTACCTGT GATCATCAAC AATAATACAG TTTCAGTGGA AGGTCCTGCC
    AATTCTTCAG AAGCTGTTCA GCATTCTGTA AATGAAGGAA TCACAGAGTT ACCTGCTTTG
    GTCTGGCCAG ATATTGAAGG ATCAGATGAA GAATTCAAAG TTTTAGATGA GGACATGGAA
    CTCGTCAAGT TTGTGCCTAC AAAAAGCCCA GTGACTGAAC TCTTAGAAGA AGATTTAGTT
    GGTGATGAAA TATTGGTTGC AACAACAACA ACAACAACAA CTCTATCCTC AACAACAGTA
    GCGACTGTGG TAACAAGTTT AGGTCATTCA GCCTCCCTGA CTCCTGAAAA GGACTCACCT
    TTCACTTGGA TATCACATTC ATCAATAGAT GAGGATGAAC CTGTGCAAGA GTCAACCACA
    GAACCTCTAC CCACCCAATC ACCCACTGCT TCTGTTCTTC CAAAGACAAC TACACAGACA
    TTGATGCACA CATACATAGC AGCTGTTGCA GGGGAGCCTG TTTTATCAGA TAACATGGAT
    AATAAGGACT CAACCACAAT GTCCACCCTT TTAGATGTTT CAAAACATTT AAAGACCCTT
    AGTTCCACCT CTGTACAATC ATTTTCAGAA CCAACATTTA CACCTACTGT CCACATAATT
    AAGCATGGAG AAACTTCAGA TGACTCAGTA CAAGATGTAG TTGAGACTGG AGAAGTTTTA
    GATGATTCTG TACAAGATGT ACTTGAGCAT GAGGACATTT TATCTGGCCC AGTTCAAGAT
    GTAGTTGTGT CTGGAGAAAC TGCAGAAGAC TCAGTACAAG ATTTAGTTGA GACTAGAGAA
    GTTTTAGATG ATTCTGTACA AGATGTACTT GAGCATGAGG ACATTTTATC TGGCCCAGTT
    CAAGATGTAG TTGTGTCTGG AGAAACTGCA GATGACTCAG TACAAGATTT AGTTGAGACT
    GGAGAAGTTT TAGATGATTC CGTACAAGAT GTACTTGAGC ATGAAGTAAT TTCATTTGGC
    CCAGTACAAG ATGTAGTTGT ATCTGGAAAA ACTTCAAATG ACTCAGTACA AGATGTAGTT
    GAGAGTGCAA AAGATTCAGA TGCCCCAATA CAAGATGTAT TTGAGCATAA AGACATTTCA
    GTTGACTCTG TACAAGATGT AGTTGTGTCT GGAGAAACTG CAGATGACTC TATTCAAGAT
    TTATTTAAGC ATGAAGGCAC TTCGTTCGAC ACAGTACAAG ATGAGGTTGA GTCTAGAGAA
    ACTTCAGATG ACTCAGTACA AAATGTAGTT GAGCATGAAG ACTCTTCAGA TGACGCAGTT
    CAAGATGTAG TCGAGTCTGG AGACACTTCA GATGACTCTG TACAAGATAT ATTTGAGTCT
    GCAGAAACTT CAGATGACTC TGTACAAGAT GTAGCTGAGA CTGGAAAAGA ATCAGATGAC
    TTAGTACAAG ATATATTTGA GAATAAAGAC ATTTCAGTTG ACTCAGTACA AGATGTAGTT
    GTATCTGGAG AGACTTCAGA TGACTCTATT CAAGGTTTAC TTGAGCATGA AGGCACTTCA
    GTCGACATAG TACAAGATGA GGTTGAGTTT AGAGAAAATT CAGATGACTC AGTACTAAAT
    GTAGTCAAGC ATGAAGATAC TTCAGATGAC TCAATACAAG ATGTAGTCGA GACAGGAGAA
    GTTTCAGATA GCTCAGTACA AGATGTAGCT GAGTCTGGAG AAACTTTAGA TGACTCTGTT
    CAAGATTTTG TTAAAACTGG AGAAATTTCC ATTGACTCAG TACACAATTT ATTTGAGCCT
    GAAAAAAACG TAGATGACTC TGTACAAGAT GTAGTTGAGA CTGGAGAAGT TTCTGTTGAT
    TCAGTACAAG ATGTAATTGA GCTTGGAGAC ACTTTAGATG ACTCTGCACA AGGAATAGTT
    GAACCTGAAG AAATGGCAGA CAACTTTATA AAGGAAAGCA CTGTAAAGCC AAGACCTGAC
    ATCAAAGGGC TTATTCTACC AACAGAGACA GTGAACTATG AAAGTGCCAC TATGAAAGTT
    CCTGCTCCAG TTAACCCCAG CATAACAGAA TTTAAGGTTT CATTTGACAT CTTCCCTTTC
    GACGACCCAA CTGATGATGA AGATAATGGC AGTGGGTTTG CTCATGGAAC TGACTTAGCA
    AGTTTAGCCT TGCCTGCCAG TCCTGGAAGG GCATTAATAG TGTTTTTTAG CCTTAGGGTC
    ACCAACATGA AGTTTTCCGA AGATCTCTTC AACAAGAGCT CTACTGAATA CAAGGCACTT
    GAGCAGCGAT TTCTGGAACT GCTCGTACCG TATCTACAGT CCAACCTCAG CAATTTCCAG
    AATTTAGAGA TTTTGAACTT CAGGAATGGT AGCATTGTGG TGAACAGCAG GATGAAGTTT
    GCCAAGCCAG TTCCCCATGG TGTGACAACA GCAGTGTACC TAATCTTGGA GGACTTCTGC
    AACACTGCCT ACCAAACTAT GAATCTTGCA ATAGACAAGT ATTCATTGGA TGTCGAATCA
    GGTGACCAGG CAGATCCTTG TAAGTTTCAG GCATGTAACG AGTTCTCCAA GTGTTTGGTG
    AACCGCTGGT CAGGTGAGGC CGAGTGTGTT TGTAACGCTG GTTATTTCAG CGTTGACGGA
    CTGCCATGCC AGAGCATCTG CGACATCCAA GAAGACTTCT GCCTAAATGA CGGGAAATGT
    GACATAATAC CTGGCAAGGG CGCCATCTGC AGGTGTCGCG TTGGTGAAAA CTGGTGGTAT
    CGAGGAGAAC ACTGTGAGGA ATATGTGTCA GAGCCGCTGG TGGTCGGCAT TGCCATTGCG
    TCCGTTGCTG GATTTCTCCT GGTGGCGTCG GGTGTCATCT TTTTCCTGGC CCGAACTCTG
    AGAGACCAGT ATGACACAGA TGATTCAGAA GACCCTTTAC GATATGCAGA GAACTTGCCA
    TCTCTAGAAA GAGCCACCAA GTACAACCCG ATGTTTGAAA GTGAAGTGAC TGCAGGCCAT
    AATCAGTATT ATCGGCGTTA CCCAGAAGCT CCGGCATTCA GCACGGGCAG CACCGAGATC
    TCCACTGACT TCAGCAGCGA AGAGATTCGT CACATCTATC AGAACAGTGA ACTTACTAAA
    GAGGAAATCC AGGACCGTAT TCGTATTATA GAGCTTTACG CCAAGGACCG CCAGTTTGCA
    GATTTTGTCC GACAGCACCA AGTGGCGCTT GACGCTAGAC GAGAGAGCTC TTCCAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146186
    Clone ID Related Accession (Same CDS sequence) XM_021473522.1 , XM_021469612.1 , XM_021474486.1
    Accession Version XM_021474486.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8340bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin homolog isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGCCTGATT TACCTTTTAA ATGCATTTGG TGGGGTTTGA CACTATTGTT GTTGACTGGA 
    TACTTTTCTA TAGATGCAGA TACTGGAACT CATGAGGATT ACTTGGCAAA TGGAAATACA
    ATGCTTTTGG ACACTAATGA AGTTTTGAAT CACTTATTGT TGTTGGACAA AGGTAAAACT
    TTGAATTACA TGTCTGCTGA AGGGAAAACA GCTCTATCTA GACATAAAAG GGACCTCCTG
    TTCCCCAATG GAGTTAAACT GTGCTCACAT GAGACGGTGC AACAGGCAGT GCAGAATCAT
    CTCAACTACT TTCACCTGCG AGTGTGCCAG GAGACAATTT GGGAGGCTTA TAAAATCTTC
    TGGGATCGCT TACCTGAGAA AGAAGAGTAC CAGATCTGGA TGAAAAAATG TCAAAATGGT
    AGCATTAGCG TGTTTGACAT CGGCCGAAGC TTTAGTCAGT CAGCTGAGCA CCTTGCTTTG
    ATTTCAAGTA GGGTAGCATC ATCCACAATA ACATCAACAA GTGCGCCCAC CAGCCCATGG
    CAACCTGAAT GCAGGCATCA GACGACGACT ACTTCGCCAA CACTAGCAAA AGCTGAACAA
    GTTGGTATTT TGATTCCTGA GGCAGTGCCA GGTTCACAAG TTGTCACACC GCAGACCTTT
    GCAGATGATA TCGTAATCAC AACTTTGCCT ACAAGGCTTC AAGTGACACC TGGAATTACC
    CCAAAGCCTG ATCTTGTCAC AGCCTTTAAG GACACAGTAA AACCTATTTC TGAAACTGAA
    CCTGAGGTCA TATTGGGTAC TACTTCTGAG AGTCCTGTTA AAGCATTGGA TACCATTACC
    ATTAAACATG AAGAGGTTGT TGTAGTCTCA GAATCTAAAT TATTGATTAC TGAGAGGACT
    TCGGAGGACT CTACTTCAAG GAAATATGAT TATGAGTTTC CCACGACCTT TAAGGACTCG
    TTAGTAATAA ACACTCCTAT AGTACTAACA ACTGAATTTT CTGAACAAAT GTATTCAGAA
    ATAGGACAAG GTTTTTCTGA TGGTACAGTT AAAACAGTAG CTGTTACTAT TCAAAAGGAA
    CTTGACATTG TCTCAAAATC TACATTATTA ACCACTGACA AAACTCCAGA AGTTTCTACT
    TCAAAAAAAT CTGAGCATGA TAATCCCACT AACTTTGTAG ATTCATTGGG AATAAGCACC
    AATACTCCTA GAGTGACCAC AAGTGAACTC AAAGAAGATC TTCATTCAGA ATTAGAACAG
    GTGGCTGGGC AGGATGTTAT TCACAGTCCT GTTAAAACAA CACATGTTAC AATTAAGCAT
    GAAGTGTTGG ACACAAATAC TACATTAATG ACAACTGACA GAACTCCATA TTCTACTTCA
    GCAAAATATG AACGTGTTAT ACCAACATCC TTTGAGGACT CACTAGTCAC AAGTTCATCT
    ACTGAACAGG ATATGACCAA TGTTCCTATT AAGACGGCAT ATGCCACAAT TTCACATGGA
    GAGTCTGAAA TTGTCACAGA GTCGACAATA ACAACCACTA ACAGAAATCT TGAAGATTCT
    TCTTTAGCAA AATATGATTA TGGGATTCCT CAAGGAATTG ATACTTCTTC GGGAGTAATT
    AATGAATCTT CTACAAAAGA ACATAATTCA GTATCTAAAT TGAATATTGG TCAGACTACT
    ACTGAAGGTC CCCAAACAAC TGATGCAGTT CAGTATGTAG AATCTGACAT TGTGTCAGAG
    GAAACGTTGA AATCCACTGG CAGAACTGCA GTTAGCTCAA ACTCTACAAA AATTGAGGCC
    GATCTTTCCA CAACTCCTGA GGACCTGACA GGAGTAAGTA CCAACACTCC TGAAATAGTC
    ACTAGAGATT CCATTACAAT ACTTATCCCA GAAACCGAGG GCATTACTAA AAATCCTGTT
    AAATCTGCAC CTATTACCAT TCAGCATGTG GATGTTGATG ATGTTTTAGC AGCTACAGTG
    ATGTCCACTC ATAAACCTCC CAAAGTCTCA CCTACAGAAA TATCCCCCGA TCAGAATCTC
    TTACCAGAGG AATCAAAGCT GCCAACTCCT ACAAAACTAG CAGAAGTTTT GTCTGCCACA
    GAAGAGTTGC CAAAGGATCA GACAGACAAG GATGTTGCTA AAATTGTATA TGTGTCTACT
    GTGCCAGAAC TGGTGTCACC AACTATTCCC GCATATACGT CAACTGACAC TACTAAGGAA
    AACCTACATG TGTTAACAGG GGGGGCAGTT GTTACATCAC TTGATAAAGA TGAGGAAGAA
    CATACACCCC ACACAAAAGA GAGCAAAATT ACACAGGTGA CAACAGGAGC AGAATTACCA
    GGTACCATAA CAGAGAGTAC TCATGGAATG TGGAGGGAAA CCCAAGAGCC AATAAATGTC
    ACACCTGATG CTGAAGACAA TCATTTGGGC AAAGAAGGGT ACATAATAAC AGAGATCCCA
    GAAGTTGCTG AAGTACCAGC TGGAGTGACA CCCAAAATGA TTGTGGAAAC TACAACAGCC
    ACTGAATCTG TGTTTACTGT GAAAGACACA TCTATAATTG TAGACAAAAC TGGCATTGTG
    AAACCTGTCG TGACTACGAC AGAGGCAGCT GAACTTACTG AGGTTGGATT CGAAGATTTA
    GGCACAGACA AAGTAACAAT TCTTGAAAAC ACACTTGAAG ATTCTGTAGG ACTAACAAAT
    GAACCTTTTG CTGTATCATG GGGTGTAACT GAAGCCACTA TTTTAGAGGA ATCATATGTG
    GAAAGTACTG AAAGTATTTC AGGTAAAGAG AAGTCCACTG AAACTATTAA GATCACAGGT
    GAAGCCACAA CTGATGACAT TGGTACTTCT GAAACTGACA TTGTAGTGCC TGTAACCGAT
    CGCATAGAAG ATAATGTAAA TGTATCACCT AAAAATGATG ATGCTAAGGA AACAAACATT
    TTTGAAGACA CAACACCAGA TCTTAGTGTT ACTAATTCTG AAAAAGACAT ACTGACAACT
    ACCATACATA AAGACATGCA TGAAGTTACA GAACCATTAA ATATTCCAGA GGTCACACAT
    AAGGTTGTGA TAGAGATCAC AGATGATGTA CGTGAGACCA GAGGAGATTC CTTACCCGAG
    ACACCAGAAG TCACAACAGA GACACCTAAG GTTGCAACAG AAGCTGATGT CAAAACAACA
    ACAAATGTTA TATCTAAAGC ACCTGAAGTT ATACTGAAGA CAACTGTAAC CAAGTCAGAT
    ACACCTGATG TCACAACTCA GACACCTTTG TTAAAGCTTG AAGATACAGA GAGGGTTGAA
    ATAGTACCTG AAACTCCTGA CATACAAGTG GTTCAAACAG AACACTCAGA AAATGATATC
    CCAACTCCTG TCTTGAAAGT GGAGGATTTC ACAGTTGCGC TGAATGACTT GACAACTCCT
    ATTCAAAAGA CTTCTTCAAC CACATTACAA CAAGACATTT CTAATGATAT TTTAGAAGAA
    AACAATGTGA TGGGAAATGA AATTGATGAT ATTGTGGTTC GACCAGTACG CCCTGTCGGC
    GATCATATTT TGGAGCTAAG TATTAAGCTA AGAGGGGAAA GCTATGACGA TGCTCTGAGG
    GATCCATCAA GTAACTACTA TCAACATCTG TCTACACTTT TTGTTGAAAA GATTGAAGAA
    GTTTTCGAAA GGCTTCCAGG TTTCAAGAAA ATATATGTTC TTGAATTCAG GCCACAGAAG
    GACATTGAAG GAGGTCTGGC AGTGGTGGTG CACTATGCTG TTGTTCTTGA GGGTGATGGG
    TCAGGAGTAA GTAATGAGAC CATGGATTAC ATAAACCTGC ATTCCAACAT GGTTGAGAAC
    TCCTTCACAG ATCCTGAGGA GCTGCCCACT GTGGTCTACA CCATCACTGA CTTCCGAAAC
    TACATCTCCG AAGCCCTTCA CAAGGATAAC TTTATCAGAA ACGCTACTCT GAATGTGGAC
    ATTGATTCTT TGCAACTTGA AAATGTGGAG ACATTGCTGC CTTCCAAACC AACCAGCAGA
    CCGTTTGACT CCAGTGACAT GATGGACAAC GTTCTTGCTT CCGAGAAACC ACCAGATGTT
    CCTGGGCAAG AATTATCCAA CAATGACATC TTCATTACAA ATTATGACAT CTTTGATCCT
    GATCCATGGA CAGGTGGACA AAGTGAAGAC AGAAATGCAA ATGATGTAAT CATCTTTGAG
    GAAAGTCCCA CTCAGTCTCC TGTTGAAGTG TCTTTGAAGA ACTTGGACAT TGAAGTTACT
    TCAAAATCTG AAACTTCAAG CAGTGCCCCT GTTCCTGATG AAGCTGTTAT TGAAGAGGAG
    GGATTTTTAC AGACTGCAAC TACAGTCAGT CCTATTATAG ACACAATAAC TGAATTTACT
    TTACCTACAG AATCACCTCA CAAGGTAGAA GAGACACCAG TTTCTCCTGT AGAACCTCCT
    GAGGTTGAGG AAACTACCCT GGGTGATTTA ACAGACCTTG GCTCAGGCTC AGGATTCTCT
    GGAGATCACA TAGGCCCTGA CATCTGGCCA TGGTCATCAG AAACATCACA AGAAGTCTTG
    AAGGAAGATA CCAATGATGA GATACACCAA CTTCAGGAAA ATCAGGAATC TATAATAAAA
    CAGCTTGAGC CTTTACATCA AGATGTGACT CCTGAGGAGC CATTCTTGGA TAGGGTTCTT
    GTGACCCAAG ACATTCGTAC CAATCCTCAT TACACAACAA CTGACCAGGC CCCTGTATTT
    TGGACAATGG AGACTTTGAC TGTTGAGCTT TCAATGCAGA CTCAAGTAGC CCCTGAACAT
    TATGATGACT ATTTTCCAGA TGCATCAATC ACTATGGCTA CACTTGTAAC CAATCAACCA
    TTGTTACATG TTCACAATCC ATTAGAAACA AAAGATCTGG ATGCATCACA AAGTCCAGGA
    ACAATAAGTT CAACTGCTGA TAAGGACTAT ACAGAACGAC TTGTTACACC CACTGTGATC
    CAACCAAGTG AAACAGTAGC TGTGGATAAG CATACTACAA CAGACAACAA ACAGAATCTG
    GAAACTATGG GTACATTATC TGCAACACTG GGCTCCACAA TGAAGTCCAC TGAAATCTCT
    TCAACTACCC AATTACCTGT GATCATCAAC AATAATACAG TTTCAGTGGA AGGTCCTGCC
    AATTCTTCAG AAGCTGTTCA GCATTCTGTA AATGAAGGAA TCACAGAGTT ACCTGCTTTG
    GTCTGGCCAG ATATTGAAGG ATCAGATGAA GAATTCAAAG TTTTAGATGA GGACATGGAA
    CTCGTCAAGT TTGTGCCTAC AAAAAGCCCA GTGACTGAAC TCTTAGAAGA AGATTTAGTT
    GGTGATGAAA TATTGGTTGC AACAACAACA ACAACAACAA CTCTATCCTC AACAACAGTA
    GCGACTGTGG TAACAAGTTT AGGTCATTCA GCCTCCCTGA CTCCTGAAAA GGACTCACCT
    TTCACTTGGA TATCACATTC ATCAATAGAT GAGGATGAAC CTGTGCAAGA GTCAACCACA
    GAACCTCTAC CCACCCAATC ACCCACTGCT TCTGTTCTTC CAAAGACAAC TACACAGACA
    TTGATGCACA CATACATAGC AGCTGTTGCA GGGGAGCCTG TTTTATCAGA TAACATGGAT
    AATAAGGACT CAACCACAAT GTCCACCCTT TTAGATGTTT CAAAACATTT AAAGACCCTT
    AGTTCCACCT CTGTACAATC ATTTTCAGAA CCAACATTTA CACCTACTGT CCACATAATT
    AAGCATGGAG AAACTTCAGA TGACTCAGTA CAAGATGTAG TTGAGACTGG AGAAGTTTTA
    GATGATTCTG TACAAGATGT ACTTGAGCAT GAGGACATTT TATCTGGCCC AGTTCAAGAT
    GTAGTTGTGT CTGGAGAAAC TGCAGAAGAC TCAGTACAAG ATTTAGTTGA GACTAGAGAA
    GTTTTAGATG ATTCTGTACA AGATGTACTT GAGCATGAGG ACATTTTATC TGGCCCAGTT
    CAAGATGTAG TTGTGTCTGG AGAAACTGCA GATGACTCAG TACAAGATTT AGTTGAGACT
    GGAGAAGTTT TAGATGATTC CGTACAAGAT GTACTTGAGC ATGAAGTAAT TTCATTTGGC
    CCAGTACAAG ATGTAGTTGT ATCTGGAAAA ACTTCAAATG ACTCAGTACA AGATGTAGTT
    GAGAGTGCAA AAGATTCAGA TGCCCCAATA CAAGATGTAT TTGAGCATAA AGACATTTCA
    GTTGACTCTG TACAAGATGT AGTTGTGTCT GGAGAAACTG CAGATGACTC TATTCAAGAT
    TTATTTAAGC ATGAAGGCAC TTCGTTCGAC ACAGTACAAG ATGAGGTTGA GTCTAGAGAA
    ACTTCAGATG ACTCAGTACA AAATGTAGTT GAGCATGAAG ACTCTTCAGA TGACGCAGTT
    CAAGATGTAG TCGAGTCTGG AGACACTTCA GATGACTCTG TACAAGATAT ATTTGAGTCT
    GCAGAAACTT CAGATGACTC TGTACAAGAT GTAGCTGAGA CTGGAAAAGA ATCAGATGAC
    TTAGTACAAG ATATATTTGA GAATAAAGAC ATTTCAGTTG ACTCAGTACA AGATGTAGTT
    GTATCTGGAG AGACTTCAGA TGACTCTATT CAAGGTTTAC TTGAGCATGA AGGCACTTCA
    GTCGACATAG TACAAGATGA GGTTGAGTTT AGAGAAAATT CAGATGACTC AGTACTAAAT
    GTAGTCAAGC ATGAAGATAC TTCAGATGAC TCAATACAAG ATGTAGTCGA GACAGGAGAA
    GTTTCAGATA GCTCAGTACA AGATGTAGCT GAGTCTGGAG AAACTTTAGA TGACTCTGTT
    CAAGATTTTG TTAAAACTGG AGAAATTTCC ATTGACTCAG TACACAATTT ATTTGAGCCT
    GAAAAAAACG TAGATGACTC TGTACAAGAT GTAGTTGAGA CTGGAGAAGT TTCTGTTGAT
    TCAGTACAAG ATGTAATTGA GCTTGGAGAC ACTTTAGATG ACTCTGCACA AGGAATAGTT
    GAACCTGAAG AAATGGCAGA CAACTTTATA AAGGAAAGCA CTGTAAAGCC AAGACCTGAC
    ATCAAAGGGC TTATTCTACC AACAGAGACA GTGAACTATG AAAGTGCCAC TATGAAAGTT
    CCTGCTCCAG TTAACCCCAG CATAACAGAA TTTAAGGTTT CATTTGACAT CTTCCCTTTC
    GACGACCCAA CTGATGATGA AGATAATGGC AGTGGGTTTG CTCATGGAAC TGACTTAGCA
    AGTTTAGCCT TGCCTGCCAG TCCTGGAAGG GCATTAATAG TGTTTTTTAG CCTTAGGGTC
    ACCAACATGA AGTTTTCCGA AGATCTCTTC AACAAGAGCT CTACTGAATA CAAGGCACTT
    GAGCAGCGAT TTCTGGAACT GCTCGTACCG TATCTACAGT CCAACCTCAG CAATTTCCAG
    AATTTAGAGA TTTTGAACTT CAGGAATGGT AGCATTGTGG TGAACAGCAG GATGAAGTTT
    GCCAAGCCAG TTCCCCATGG TGTGACAACA GCAGTGTACC TAATCTTGGA GGACTTCTGC
    AACACTGCCT ACCAAACTAT GAATCTTGCA ATAGACAAGT ATTCATTGGA TGTCGAATCA
    GGTGACCAGG CAGATCCTTG TAAGTTTCAG GCATGTAACG AGTTCTCCAA GTGTTTGGTG
    AACCGCTGGT CAGGTGAGGC CGAGTGTGTT TGTAACGCTG GTTATTTCAG CGTTGACGGA
    CTGCCATGCC AGAGCATCTG CGACATCCAA GAAGACTTCT GCCTAAATGA CGGGAAATGT
    GACATAATAC CTGGCAAGGG CGCCATCTGC AGGTGTCGCG TTGGTGAAAA CTGGTGGTAT
    CGAGGAGAAC ACTGTGAGGA ATATGTGTCA GAGCCGCTGG TGGTCGGCAT TGCCATTGCG
    TCCGTTGCTG GATTTCTCCT GGTGGCGTCG GGTGTCATCT TTTTCCTGGC CCGAACTCTG
    AGAGACCAGT ATGACACAGA TGATTCAGAA GACCCTTTAC GATATGCAGA GAACTTGCCA
    TCTCTAGAAA GAGCCACCAA GTACAACCCG ATGTTTGAAA GTGAAGTGAC TGCAGGCCAT
    AATCAGTATT ATCGGCGTTA CCCAGAAGCT CCGGCATTCA GCACGGGCAG CACCGAGATC
    TCCACTGACT TCAGCAGCGA AGAGATTCGT CACATCTATC AGAACAGTGA ACTTACTAAA
    GAGGAAATCC AGGACCGTAT TCGTATTATA GAGCTTTACG CCAAGGACCG CCAGTTTGCA
    GATTTTGTCC GACAGCACCA AGTGGCGCTT GACGCTAGAC GAGAGAGCTC TTCCAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330161.1
    CDS233..8572
    Translation

    Target ORF information:

    RefSeq Version XM_021474486.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio interphotoreceptor matrix proteoglycan 2b (impg2b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474486.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGCCTGATT TACCTTTTAA ATGCATTTGG TGGGGTTTGA CACTATTGTT GTTGACTGGA 
    TACTTTTCTA TAGATGCAGA TACTGGAACT CATGAGGATT ACTTGGCAAA TGGAAATACA
    ATGCTTTTGG ACACTAATGA AGTTTTGAAT CACTTATTGT TGTTGGACAA AGGTAAAACT
    TTGAATTACA TGTCTGCTGA AGGGAAAACA GCTCTATCTA GACATAAAAG GGACCTCCTG
    TTCCCCAATG GAGTTAAACT GTGCTCACAT GAGACGGTGC AACAGGCAGT GCAGAATCAT
    CTCAACTACT TTCACCTGCG AGTGTGCCAG GAGACAATTT GGGAGGCTTA TAAAATCTTC
    TGGGATCGCT TACCTGAGAA AGAAGAGTAC CAGATCTGGA TGAAAAAATG TCAAAATGGT
    AGCATTAGCG TGTTTGACAT CGGCCGAAGC TTTAGTCAGT CAGCTGAGCA CCTTGCTTTG
    ATTTCAAGTA GGGTAGCATC ATCCACAATA ACATCAACAA GTGCGCCCAC CAGCCCATGG
    CAACCTGAAT GCAGGCATCA GACGACGACT ACTTCGCCAA CACTAGCAAA AGCTGAACAA
    GTTGGTATTT TGATTCCTGA GGCAGTGCCA GGTTCACAAG TTGTCACACC GCAGACCTTT
    GCAGATGATA TCGTAATCAC AACTTTGCCT ACAAGGCTTC AAGTGACACC TGGAATTACC
    CCAAAGCCTG ATCTTGTCAC AGCCTTTAAG GACACAGTAA AACCTATTTC TGAAACTGAA
    CCTGAGGTCA TATTGGGTAC TACTTCTGAG AGTCCTGTTA AAGCATTGGA TACCATTACC
    ATTAAACATG AAGAGGTTGT TGTAGTCTCA GAATCTAAAT TATTGATTAC TGAGAGGACT
    TCGGAGGACT CTACTTCAAG GAAATATGAT TATGAGTTTC CCACGACCTT TAAGGACTCG
    TTAGTAATAA ACACTCCTAT AGTACTAACA ACTGAATTTT CTGAACAAAT GTATTCAGAA
    ATAGGACAAG GTTTTTCTGA TGGTACAGTT AAAACAGTAG CTGTTACTAT TCAAAAGGAA
    CTTGACATTG TCTCAAAATC TACATTATTA ACCACTGACA AAACTCCAGA AGTTTCTACT
    TCAAAAAAAT CTGAGCATGA TAATCCCACT AACTTTGTAG ATTCATTGGG AATAAGCACC
    AATACTCCTA GAGTGACCAC AAGTGAACTC AAAGAAGATC TTCATTCAGA ATTAGAACAG
    GTGGCTGGGC AGGATGTTAT TCACAGTCCT GTTAAAACAA CACATGTTAC AATTAAGCAT
    GAAGTGTTGG ACACAAATAC TACATTAATG ACAACTGACA GAACTCCATA TTCTACTTCA
    GCAAAATATG AACGTGTTAT ACCAACATCC TTTGAGGACT CACTAGTCAC AAGTTCATCT
    ACTGAACAGG ATATGACCAA TGTTCCTATT AAGACGGCAT ATGCCACAAT TTCACATGGA
    GAGTCTGAAA TTGTCACAGA GTCGACAATA ACAACCACTA ACAGAAATCT TGAAGATTCT
    TCTTTAGCAA AATATGATTA TGGGATTCCT CAAGGAATTG ATACTTCTTC GGGAGTAATT
    AATGAATCTT CTACAAAAGA ACATAATTCA GTATCTAAAT TGAATATTGG TCAGACTACT
    ACTGAAGGTC CCCAAACAAC TGATGCAGTT CAGTATGTAG AATCTGACAT TGTGTCAGAG
    GAAACGTTGA AATCCACTGG CAGAACTGCA GTTAGCTCAA ACTCTACAAA AATTGAGGCC
    GATCTTTCCA CAACTCCTGA GGACCTGACA GGAGTAAGTA CCAACACTCC TGAAATAGTC
    ACTAGAGATT CCATTACAAT ACTTATCCCA GAAACCGAGG GCATTACTAA AAATCCTGTT
    AAATCTGCAC CTATTACCAT TCAGCATGTG GATGTTGATG ATGTTTTAGC AGCTACAGTG
    ATGTCCACTC ATAAACCTCC CAAAGTCTCA CCTACAGAAA TATCCCCCGA TCAGAATCTC
    TTACCAGAGG AATCAAAGCT GCCAACTCCT ACAAAACTAG CAGAAGTTTT GTCTGCCACA
    GAAGAGTTGC CAAAGGATCA GACAGACAAG GATGTTGCTA AAATTGTATA TGTGTCTACT
    GTGCCAGAAC TGGTGTCACC AACTATTCCC GCATATACGT CAACTGACAC TACTAAGGAA
    AACCTACATG TGTTAACAGG GGGGGCAGTT GTTACATCAC TTGATAAAGA TGAGGAAGAA
    CATACACCCC ACACAAAAGA GAGCAAAATT ACACAGGTGA CAACAGGAGC AGAATTACCA
    GGTACCATAA CAGAGAGTAC TCATGGAATG TGGAGGGAAA CCCAAGAGCC AATAAATGTC
    ACACCTGATG CTGAAGACAA TCATTTGGGC AAAGAAGGGT ACATAATAAC AGAGATCCCA
    GAAGTTGCTG AAGTACCAGC TGGAGTGACA CCCAAAATGA TTGTGGAAAC TACAACAGCC
    ACTGAATCTG TGTTTACTGT GAAAGACACA TCTATAATTG TAGACAAAAC TGGCATTGTG
    AAACCTGTCG TGACTACGAC AGAGGCAGCT GAACTTACTG AGGTTGGATT CGAAGATTTA
    GGCACAGACA AAGTAACAAT TCTTGAAAAC ACACTTGAAG ATTCTGTAGG ACTAACAAAT
    GAACCTTTTG CTGTATCATG GGGTGTAACT GAAGCCACTA TTTTAGAGGA ATCATATGTG
    GAAAGTACTG AAAGTATTTC AGGTAAAGAG AAGTCCACTG AAACTATTAA GATCACAGGT
    GAAGCCACAA CTGATGACAT TGGTACTTCT GAAACTGACA TTGTAGTGCC TGTAACCGAT
    CGCATAGAAG ATAATGTAAA TGTATCACCT AAAAATGATG ATGCTAAGGA AACAAACATT
    TTTGAAGACA CAACACCAGA TCTTAGTGTT ACTAATTCTG AAAAAGACAT ACTGACAACT
    ACCATACATA AAGACATGCA TGAAGTTACA GAACCATTAA ATATTCCAGA GGTCACACAT
    AAGGTTGTGA TAGAGATCAC AGATGATGTA CGTGAGACCA GAGGAGATTC CTTACCCGAG
    ACACCAGAAG TCACAACAGA GACACCTAAG GTTGCAACAG AAGCTGATGT CAAAACAACA
    ACAAATGTTA TATCTAAAGC ACCTGAAGTT ATACTGAAGA CAACTGTAAC CAAGTCAGAT
    ACACCTGATG TCACAACTCA GACACCTTTG TTAAAGCTTG AAGATACAGA GAGGGTTGAA
    ATAGTACCTG AAACTCCTGA CATACAAGTG GTTCAAACAG AACACTCAGA AAATGATATC
    CCAACTCCTG TCTTGAAAGT GGAGGATTTC ACAGTTGCGC TGAATGACTT GACAACTCCT
    ATTCAAAAGA CTTCTTCAAC CACATTACAA CAAGACATTT CTAATGATAT TTTAGAAGAA
    AACAATGTGA TGGGAAATGA AATTGATGAT ATTGTGGTTC GACCAGTACG CCCTGTCGGC
    GATCATATTT TGGAGCTAAG TATTAAGCTA AGAGGGGAAA GCTATGACGA TGCTCTGAGG
    GATCCATCAA GTAACTACTA TCAACATCTG TCTACACTTT TTGTTGAAAA GATTGAAGAA
    GTTTTCGAAA GGCTTCCAGG TTTCAAGAAA ATATATGTTC TTGAATTCAG GCCACAGAAG
    GACATTGAAG GAGGTCTGGC AGTGGTGGTG CACTATGCTG TTGTTCTTGA GGGTGATGGG
    TCAGGAGTAA GTAATGAGAC CATGGATTAC ATAAACCTGC ATTCCAACAT GGTTGAGAAC
    TCCTTCACAG ATCCTGAGGA GCTGCCCACT GTGGTCTACA CCATCACTGA CTTCCGAAAC
    TACATCTCCG AAGCCCTTCA CAAGGATAAC TTTATCAGAA ACGCTACTCT GAATGTGGAC
    ATTGATTCTT TGCAACTTGA AAATGTGGAG ACATTGCTGC CTTCCAAACC AACCAGCAGA
    CCGTTTGACT CCAGTGACAT GATGGACAAC GTTCTTGCTT CCGAGAAACC ACCAGATGTT
    CCTGGGCAAG AATTATCCAA CAATGACATC TTCATTACAA ATTATGACAT CTTTGATCCT
    GATCCATGGA CAGGTGGACA AAGTGAAGAC AGAAATGCAA ATGATGTAAT CATCTTTGAG
    GAAAGTCCCA CTCAGTCTCC TGTTGAAGTG TCTTTGAAGA ACTTGGACAT TGAAGTTACT
    TCAAAATCTG AAACTTCAAG CAGTGCCCCT GTTCCTGATG AAGCTGTTAT TGAAGAGGAG
    GGATTTTTAC AGACTGCAAC TACAGTCAGT CCTATTATAG ACACAATAAC TGAATTTACT
    TTACCTACAG AATCACCTCA CAAGGTAGAA GAGACACCAG TTTCTCCTGT AGAACCTCCT
    GAGGTTGAGG AAACTACCCT GGGTGATTTA ACAGACCTTG GCTCAGGCTC AGGATTCTCT
    GGAGATCACA TAGGCCCTGA CATCTGGCCA TGGTCATCAG AAACATCACA AGAAGTCTTG
    AAGGAAGATA CCAATGATGA GATACACCAA CTTCAGGAAA ATCAGGAATC TATAATAAAA
    CAGCTTGAGC CTTTACATCA AGATGTGACT CCTGAGGAGC CATTCTTGGA TAGGGTTCTT
    GTGACCCAAG ACATTCGTAC CAATCCTCAT TACACAACAA CTGACCAGGC CCCTGTATTT
    TGGACAATGG AGACTTTGAC TGTTGAGCTT TCAATGCAGA CTCAAGTAGC CCCTGAACAT
    TATGATGACT ATTTTCCAGA TGCATCAATC ACTATGGCTA CACTTGTAAC CAATCAACCA
    TTGTTACATG TTCACAATCC ATTAGAAACA AAAGATCTGG ATGCATCACA AAGTCCAGGA
    ACAATAAGTT CAACTGCTGA TAAGGACTAT ACAGAACGAC TTGTTACACC CACTGTGATC
    CAACCAAGTG AAACAGTAGC TGTGGATAAG CATACTACAA CAGACAACAA ACAGAATCTG
    GAAACTATGG GTACATTATC TGCAACACTG GGCTCCACAA TGAAGTCCAC TGAAATCTCT
    TCAACTACCC AATTACCTGT GATCATCAAC AATAATACAG TTTCAGTGGA AGGTCCTGCC
    AATTCTTCAG AAGCTGTTCA GCATTCTGTA AATGAAGGAA TCACAGAGTT ACCTGCTTTG
    GTCTGGCCAG ATATTGAAGG ATCAGATGAA GAATTCAAAG TTTTAGATGA GGACATGGAA
    CTCGTCAAGT TTGTGCCTAC AAAAAGCCCA GTGACTGAAC TCTTAGAAGA AGATTTAGTT
    GGTGATGAAA TATTGGTTGC AACAACAACA ACAACAACAA CTCTATCCTC AACAACAGTA
    GCGACTGTGG TAACAAGTTT AGGTCATTCA GCCTCCCTGA CTCCTGAAAA GGACTCACCT
    TTCACTTGGA TATCACATTC ATCAATAGAT GAGGATGAAC CTGTGCAAGA GTCAACCACA
    GAACCTCTAC CCACCCAATC ACCCACTGCT TCTGTTCTTC CAAAGACAAC TACACAGACA
    TTGATGCACA CATACATAGC AGCTGTTGCA GGGGAGCCTG TTTTATCAGA TAACATGGAT
    AATAAGGACT CAACCACAAT GTCCACCCTT TTAGATGTTT CAAAACATTT AAAGACCCTT
    AGTTCCACCT CTGTACAATC ATTTTCAGAA CCAACATTTA CACCTACTGT CCACATAATT
    AAGCATGGAG AAACTTCAGA TGACTCAGTA CAAGATGTAG TTGAGACTGG AGAAGTTTTA
    GATGATTCTG TACAAGATGT ACTTGAGCAT GAGGACATTT TATCTGGCCC AGTTCAAGAT
    GTAGTTGTGT CTGGAGAAAC TGCAGAAGAC TCAGTACAAG ATTTAGTTGA GACTAGAGAA
    GTTTTAGATG ATTCTGTACA AGATGTACTT GAGCATGAGG ACATTTTATC TGGCCCAGTT
    CAAGATGTAG TTGTGTCTGG AGAAACTGCA GATGACTCAG TACAAGATTT AGTTGAGACT
    GGAGAAGTTT TAGATGATTC CGTACAAGAT GTACTTGAGC ATGAAGTAAT TTCATTTGGC
    CCAGTACAAG ATGTAGTTGT ATCTGGAAAA ACTTCAAATG ACTCAGTACA AGATGTAGTT
    GAGAGTGCAA AAGATTCAGA TGCCCCAATA CAAGATGTAT TTGAGCATAA AGACATTTCA
    GTTGACTCTG TACAAGATGT AGTTGTGTCT GGAGAAACTG CAGATGACTC TATTCAAGAT
    TTATTTAAGC ATGAAGGCAC TTCGTTCGAC ACAGTACAAG ATGAGGTTGA GTCTAGAGAA
    ACTTCAGATG ACTCAGTACA AAATGTAGTT GAGCATGAAG ACTCTTCAGA TGACGCAGTT
    CAAGATGTAG TCGAGTCTGG AGACACTTCA GATGACTCTG TACAAGATAT ATTTGAGTCT
    GCAGAAACTT CAGATGACTC TGTACAAGAT GTAGCTGAGA CTGGAAAAGA ATCAGATGAC
    TTAGTACAAG ATATATTTGA GAATAAAGAC ATTTCAGTTG ACTCAGTACA AGATGTAGTT
    GTATCTGGAG AGACTTCAGA TGACTCTATT CAAGGTTTAC TTGAGCATGA AGGCACTTCA
    GTCGACATAG TACAAGATGA GGTTGAGTTT AGAGAAAATT CAGATGACTC AGTACTAAAT
    GTAGTCAAGC ATGAAGATAC TTCAGATGAC TCAATACAAG ATGTAGTCGA GACAGGAGAA
    GTTTCAGATA GCTCAGTACA AGATGTAGCT GAGTCTGGAG AAACTTTAGA TGACTCTGTT
    CAAGATTTTG TTAAAACTGG AGAAATTTCC ATTGACTCAG TACACAATTT ATTTGAGCCT
    GAAAAAAACG TAGATGACTC TGTACAAGAT GTAGTTGAGA CTGGAGAAGT TTCTGTTGAT
    TCAGTACAAG ATGTAATTGA GCTTGGAGAC ACTTTAGATG ACTCTGCACA AGGAATAGTT
    GAACCTGAAG AAATGGCAGA CAACTTTATA AAGGAAAGCA CTGTAAAGCC AAGACCTGAC
    ATCAAAGGGC TTATTCTACC AACAGAGACA GTGAACTATG AAAGTGCCAC TATGAAAGTT
    CCTGCTCCAG TTAACCCCAG CATAACAGAA TTTAAGGTTT CATTTGACAT CTTCCCTTTC
    GACGACCCAA CTGATGATGA AGATAATGGC AGTGGGTTTG CTCATGGAAC TGACTTAGCA
    AGTTTAGCCT TGCCTGCCAG TCCTGGAAGG GCATTAATAG TGTTTTTTAG CCTTAGGGTC
    ACCAACATGA AGTTTTCCGA AGATCTCTTC AACAAGAGCT CTACTGAATA CAAGGCACTT
    GAGCAGCGAT TTCTGGAACT GCTCGTACCG TATCTACAGT CCAACCTCAG CAATTTCCAG
    AATTTAGAGA TTTTGAACTT CAGGAATGGT AGCATTGTGG TGAACAGCAG GATGAAGTTT
    GCCAAGCCAG TTCCCCATGG TGTGACAACA GCAGTGTACC TAATCTTGGA GGACTTCTGC
    AACACTGCCT ACCAAACTAT GAATCTTGCA ATAGACAAGT ATTCATTGGA TGTCGAATCA
    GGTGACCAGG CAGATCCTTG TAAGTTTCAG GCATGTAACG AGTTCTCCAA GTGTTTGGTG
    AACCGCTGGT CAGGTGAGGC CGAGTGTGTT TGTAACGCTG GTTATTTCAG CGTTGACGGA
    CTGCCATGCC AGAGCATCTG CGACATCCAA GAAGACTTCT GCCTAAATGA CGGGAAATGT
    GACATAATAC CTGGCAAGGG CGCCATCTGC AGGTGTCGCG TTGGTGAAAA CTGGTGGTAT
    CGAGGAGAAC ACTGTGAGGA ATATGTGTCA GAGCCGCTGG TGGTCGGCAT TGCCATTGCG
    TCCGTTGCTG GATTTCTCCT GGTGGCGTCG GGTGTCATCT TTTTCCTGGC CCGAACTCTG
    AGAGACCAGT ATGACACAGA TGATTCAGAA GACCCTTTAC GATATGCAGA GAACTTGCCA
    TCTCTAGAAA GAGCCACCAA GTACAACCCG ATGTTTGAAA GTGAAGTGAC TGCAGGCCAT
    AATCAGTATT ATCGGCGTTA CCCAGAAGCT CCGGCATTCA GCACGGGCAG CACCGAGATC
    TCCACTGACT TCAGCAGCGA AGAGATTCGT CACATCTATC AGAACAGTGA ACTTACTAAA
    GAGGAAATCC AGGACCGTAT TCGTATTATA GAGCTTTACG CCAAGGACCG CCAGTTTGCA
    GATTTTGTCC GACAGCACCA AGTGGCGCTT GACGCTAGAC GAGAGAGCTC TTCCAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146187
    Clone ID Related Accession (Same CDS sequence) XM_021474487.1 , XM_021469613.1 , XM_021473523.1
    Accession Version XM_021474487.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8340bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin homolog isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGCCTGATT TACCTTTTAA ATGCATTTGG TGGGGTTTGA CACTATTGTT GTTGACTGGA 
    TACTTTTCTA TAGATGCAGA TACTGGAACT CATGAGGATT ACTTGGCAAA TGGAAATACA
    ATGCTTTTGG ACACTAATGA AGTTTTGAAT CACTTATTGT TGTTGGACAA AGGTAAAACT
    TTGAATTACA TGTCTGCTGA AGGGAAAACA GCTCTATCTA GACATAAAAG GGACCTCCTG
    TTCCCCAATG GAGTTAAACT GTGCTCACAT GAGACGGTGC AACAGGCAGT GCAGAATCAT
    CTCAACTACT TTCACCTGCG AGTGTGCCAG GAGACAATTT GGGAGGCTTA TAAAATCTTC
    TGGGATCGCT TACCTGAGAA AGAAGAGTAC CAGATCTGGA TGAAAAAATG TCAAAATGGT
    AGCATTAGCG TGTTTGACAT CGGCCGAAGC TTTAGTCAGT CAGCTGAGCA CCTTGCTTTG
    ATTTCAAGTA GGGTAGCATC ATCCACAATA ACATCAACAA GTGCGCCCAC CAGCCCATGG
    CAACCTGAAT GCAGGCATCA GACGACGACT ACTTCGCCAA CACTAGCAAA AGCTGAACAA
    GTTGGTATTT TGATTCCTGA GGCAGTGCCA GGTTCACAAG TTGTCACACC GCAGACCTTT
    GCAGATGATA TCGTAATCAC AACTTTGCCT ACAAGGCTTC AAGTGACACC TGGAATTACC
    CCAAAGCCTG ATCTTGTCAC AGCCTTTAAG GACACAGTAA AACCTATTTC TGAAACTGAA
    CCTGAGGTCA TATTGGGTAC TACTTCTGAG AGTCCTGTTA AAGCATTGGA TACCATTACC
    ATTAAACATG AAGAGGTTGT TGTAGTCTCA GAATCTAAAT TATTGATTAC TGAGAGGACT
    TCGGAGGACT CTACTTCAAG GAAATATGAT TATGAGTTTC CCACGACCTT TAAGGACTCG
    TTAGTAATAA ACACTCCTAT AGTACTAACA ACTGAATTTT CTGAACAAAT GTATTCAGAA
    ATAGGACAAG GTTTTTCTGA TGGTACAGTT AAAACAGTAG CTGTTACTAT TCAAAAGGAA
    CTTGACATTG TCTCAAAATC TACATTATTA ACCACTGACA AAACTCCAGA AGTTTCTACT
    TCAAAAAAAT CTGAGCATGA TAATCCCACT AACTTTGTAG ATTCATTGGG AATAAGCACC
    AATACTCCTA GAGTGACCAC AAGTGAACTC AAAGAAGATC TTCATTCAGA ATTAGAACAG
    GTGGCTGGGC AGGATGTTAT TCACAGTCCT GTTAAAACAA CACATGTTAC AATTAAGCAT
    GAAGTGTTGG ACACAAATAC TACATTAATG ACAACTGACA GAACTCCATA TTCTACTTCA
    GCAAAATATG AACGTGTTAT ACCAACATCC TTTGAGGACT CACTAGTCAC AAGTTCATCT
    ACTGAACAGG ATATGACCAA TGTTCCTATT AAGACGGCAT ATGCCACAAT TTCACATGGA
    GAGTCTGAAA TTGTCACAGA GTCGACAATA ACAACCACTA ACAGAAATCT TGAAGATTCT
    TCTTTAGCAA AATATGATTA TGGGATTCCT CAAGGAATTG ATACTTCTTC GGGAGTAATT
    AATGAATCTT CTACAAAAGA ACATAATTCA GTATCTAAAT TGAATATTGG TCAGACTACT
    ACTGAAGGTC CCCAAACAAC TGATGCAGTT CAGTATGTAG AATCTGACAT TGTGTCAGAG
    GAAACGTTGA AATCCACTGG CAGAACTGCA GTTAGCTCAA ACTCTACAAA AATTGAGGCC
    GATCTTTCCA CAACTCCTGA GGACCTGACA GGAGTAAGTA CCAACACTCC TGAAATAGTC
    ACTAGAGATT CCATTACAAT ACTTATCCCA GAAACCGAGG GCATTACTAA AAATCCTGTT
    AAATCTGCAC CTATTACCAT TCAGCATGTG GATGTTGATG ATGTTTTAGC AGCTACAGTG
    ATGTCCACTC ATAAACCTCC CAAAGTCTCA CCTACAGAAA TATCCCCCGA TCAGAATCTC
    TTACCAGAGG AATCAAAGCT GCCAACTCCT ACAAAACTAG CAGAAGTTTT GTCTGCCACA
    GAAGAGTTGC CAAAGGATCA GACAGACAAG GATGTTGCTA AAATTGTATA TGTGTCTACT
    GTGCCAGAAC TGGTGTCACC AACTATTCCC GCATATACGT CAACTGACAC TACTAAGGAA
    AACCTACATG TGTTAACAGG GGGGGCAGTT GTTACATCAC TTGATAAAGA TGAGGAAGAA
    CATACACCCC ACACAAAAGA GAGCAAAATT ACACAGGTGA CAACAGGAGC AGAATTACCA
    GGTACCATAA CAGAGAGTAC TCATGGAATG TGGAGGGAAA CCCAAGAGCC AATAAATGTC
    ACACCTGATG CTGAAGACAA TCATTTGGGC AAAGAAGGGT ACATAATAAC AGAGATCCCA
    GAAGTTGCTG AAGTACCAGC TGGAGTGACA CCCAAAATGA TTGTGGAAAC TACAACAGCC
    ACTGAATCTG TGTTTACTGT GAAAGACACA TCTATAATTG TAGACAAAAC TGGCATTGTG
    AAACCTGTCG TGACTACGAC AGAGGCAGCT GAACTTACTG AGGTTGGATT CGAAGATTTA
    GGCACAGACA AAGTAACAAT TCTTGAAAAC ACACTTGAAG ATTCTGTAGG ACTAACAAAT
    GAACCTTTTG CTGTATCATG GGGTGTAACT GAAGCCACTA TTTTAGAGGA ATCATATGTG
    GAAAGTACTG AAAGTATTTC AGGTAAAGAG AAGTCCACTG AAACTATTAA GATCACAGGT
    GAAGCCACAA CTGATGACAT TGGTACTTCT GAAACTGACA TTGTAGTGCC TGTAACCGAT
    CGCATAGAAG ATAATGTAAA TGTATCACCT AAAAATGATG ATGCTAAGGA AACAAACATT
    TTTGAAGACA CAACACCAGA TCTTAGTGTT ACTAATTCTG AAAAAGACAT ACTGACAACT
    ACCATACATA AAGACATGCA TGAAGTTACA GAACCATTAA ATATTCCAGA GGTCACACAT
    AAGGTTGTGA TAGAGATCAC AGATGATGTA CGTGAGACCA GAGGAGATTC CTTACCCGAG
    ACACCAGAAG TCACAACAGA GACACCTAAG GTTGCAACAG AAGCTGATGT CAAAACAACA
    ACAAATGTTA TATCTAAAGC ACCTGAAGTT ATACTGAAGA CAACTGTAAC CAAGTCAGAT
    ACACCTGATG TCACAACTCA GACACCTTTG TTAAAGCTTG AAGATACAGA GAGGGTTGAA
    ATAGTACCTG AAACTCCTGA CATACAAGTG GTTCAAACAG AACACTCAGA AAATGATATC
    CCAACTCCTG TCTTGAAAGT GGAGGATTTC ACAGTTGCGC TGAATGACTT GACAACTCCT
    ATTCAAAAGA CTTCTTCAAC CACATTACAA CAAGACATTT CTAATGATAT TTTAGAAGAA
    AACAATGTGA TGGGAAATGA AATTGATGAT ATTGTGGTTC GACCAGTACG CCCTGTCGGC
    GATCATATTT TGGAGCTAAG TATTAAGCTA AGAGGGGAAA GCTATGACGA TGCTCTGAGG
    GATCCATCAA GTAACTACTA TCAACATCTG TCTACACTTT TTGTTGAAAA GATTGAAGAA
    GTTTTCGAAA GGCTTCCAGG TTTCAAGAAA ATATATGTTC TTGAATTCAG GCCACAGAAG
    GACATTGAAG GAGGTCTGGC AGTGGTGGTG CACTATGCTG TTGTTCTTGA GGGTGATGGG
    TCAGGAGTAA GTAATGAGAC CATGGATTAC ATAAACCTGC ATTCCAACAT GGTTGAGAAC
    TCCTTCACAG ATCCTGAGGA GCTGCCCACT GTGGTCTACA CCATCACTGA CTTCCGAAAC
    TACATCTCCG AAGCCCTTCA CAAGGATAAC TTTATCAGAA ACGCTACTCT GAATGTGGAC
    ATTGATTCTT TGCAACTTGA AAATGTGGAG ACATTGCTGC CTTCCAAACC AACCAGCAGA
    CCGTTTGACT CCAGTGACAT GATGGACAAC GTTCTTGCTT CCGAGAAACC ACCAGATGTT
    CCTGGGCAAG AATTATCCAA CAATGACATC TTCATTACAA ATTATGACAT CTTTGATCCT
    GATCCATGGA CAGGTGGACA AAGTGAAGAC AGAAATGCAA ATGATGTAAT CATCTTTGAG
    GAAAGTCCCA CTCAGTCTCC TGTTGAAGTG TCTTTGAAGA ACTTGGACAT TGAAGTTACT
    TCAAAATCTG AAACTTCAAG CAGTGCCCCT GTTCCTGATG AAGCTGTTAT TGAAGAGGAG
    GGATTTTTAC AGACTGCAAC TACAGTCAGT CCTATTATAG ACACAATAAC TGAATTTACT
    TTACCTACAG AATCACCTCA CAAGGTAGAA GAGACACCAG TTTCTCCTGT AGAACCTCCT
    GAGGTTGAGG AAACTACCCT GGGTGATTTA ACAGACCTTG GCTCAGGCTC AGGATTCTCT
    GGAGATCACA TAGGCCCTGA CATCTGGCCA TGGTCATCAG AAACATCACA AGAAGTCTTG
    AAGGAAGATA CCAATGATGA GATACACCAA CTTCAGGAAA ATCAGGAATC TATAATAAAA
    CAGCTTGAGC CTTTACATCA AGATGTGACT CCTGAGGAGC CATTCTTGGA TAGGGTTCTT
    GTGACCCAAG ACATTCGTAC CAATCCTCAT TACACAACAA CTGACCAGGC CCCTGTATTT
    TGGACAATGG AGACTTTGAC TGTTGAGCTT TCAATGCAGA CTCAAGTAGC CCCTGAACAT
    TATGATGACT ATTTTCCAGA TGCATCAATC ACTATGGCTA CACTTGTAAC CAATCAACCA
    TTGTTACATG TTCACAATCC ATTAGAAACA AAAGATCTGG ATGCATCACA AAGTCCAGGA
    ACAATAAGTT CAACTGCTGA TAAGGACTAT ACAGAACGAC TTGTTACACC CACTGTGATC
    CAACCAAGTG AAACAGTAGC TGTGGATAAG CATACTACAA CAGACAACAA ACAGAATCTG
    GAAACTATGG GTACATTATC TGCAACACTG GGCTCCACAA TGAAGTCCAC TGAAATCTCT
    TCAACTACCC AATTACCTGT GATCATCAAC AATAATACAG TTTCAGTGGA AGGTCCTGCC
    AATTCTTCAG AAGCTGTTCA GCATTCTGTA AATGAAGGAA TCACAGAGTT ACCTGCTTTG
    GTCTGGCCAG ATATTGAAGG ATCAGATGAA GAATTCAAAG TTTTAGATGA GGACATGGAA
    CTCGTCAAGT TTGTGCCTAC AAAAAGCCCA GTGACTGAAC TCTTAGAAGA AGATTTAGTT
    GGTGATGAAA TATTGGTTGC AACAACAACA ACAACAACAA CTCTATCCTC AACAACAGTA
    GCGACTGTGG TAACAAGTTT AGGTCATTCA GCCTCCCTGA CTCCTGAAAA GGACTCACCT
    TTCACTTGGA TATCACATTC ATCAATAGAT GAGGATGAAC CTGTGCAAGA GTCAACCACA
    GAACCTCTAC CCACCCAATC ACCCACTGCT TCTGTTCTTC CAAAGACAAC TACACAGACA
    TTGATGCACA CATACATAGC AGCTGTTGCA GGGGAGCCTG TTTTATCAGA TAACATGGAT
    AATAAGGACT CAACCACAAT GTCCACCCTT TTAGATGTTT CAAAACATTT AAAGACCCTT
    AGTTCCACCT CTGTACAATC ATTTTCAGAA CCAACATTTA CACCTACTGT CCACATAATT
    AAGCATGGAG AAACTTCAGA TGACTCAGTA CAAGATGTAG TTGAGACTGG AGAAGTTTTA
    GATGACTCTG TACAAGATGT ACTTGAGCAT GAAGACATTT CATATGGCTC TGTACAAGAT
    GTAGTTGTAT CTGGAGAAAC TTCAAATGAC TCAGTACAAG ATGTAGTTGA GACTGGAGAA
    GTTTTAGATG ATTCTGTACA AGATGTACTT GAGCATGAGG ACATTTTATC TGGCCCAGTT
    CAAGATGTAG TTGTGTCTGG AGAAACTGCA GATGACTCAG TACAAGATTT AGTTGAGACT
    GGAGAAGTTT TAGATGATTC CGTACAAGAT GTACTTGAGC ATGAAGTAAT TTCATTTGGC
    CCAGTACAAG ATGTAGTTGT ATCTGGAAAA ACTTCAAATG ACTCAGTACA AGATGTAGTT
    GAGAGTGCAA AAGATTCAGA TGCCCCAATA CAAGATGTAT TTGAGCATAA AGACATTTCA
    GTTGACTCTG TACAAGATGT AGTTGTGTCT GGAGAAACTG CAGATGACTC TATTCAAGAT
    TTATTTAAGC ATGAAGGCAC TTCGTTCGAC ACAGTACAAG ATGAGGTTGA GTCTAGAGAA
    ACTTCAGATG ACTCAGTACA AAATGTAGTT GAGCATGAAG ACTCTTCAGA TGACGCAGTT
    CAAGATGTAG TCGAGTCTGG AGACACTTCA GATGACTCTG TACAAGATAT ATTTGAGTCT
    GCAGAAACTT CAGATGACTC TGTACAAGAT GTAGCTGAGA CTGGAAAAGA ATCAGATGAC
    TTAGTACAAG ATATATTTGA GAATAAAGAC ATTTCAGTTG ACTCAGTACA AGATGTAGTT
    GTATCTGGAG AGACTTCAGA TGACTCTATT CAAGGTTTAC TTGAGCATGA AGGCACTTCA
    GTCGACATAG TACAAGATGA GGTTGAGTTT AGAGAAAATT CAGATGACTC AGTACTAAAT
    GTAGTCAAGC ATGAAGATAC TTCAGATGAC TCAATACAAG ATGTAGTCGA GACAGGAGAA
    GTTTCAGATA GCTCAGTACA AGATGTAGCT GAGTCTGGAG AAACTTTAGA TGACTCTGTT
    CAAGATTTTG TTAAAACTGG AGAAATTTCC ATTGACTCAG TACACAATTT ATTTGAGCCT
    GAAAAAAACG TAGATGACTC TGTACAAGAT GTAGTTGAGA CTGGAGAAGT TTCTGTTGAT
    TCAGTACAAG ATGTAATTGA GCTTGGAGAC ACTTTAGATG ACTCTGCACA AGGAATAGTT
    GAACCTGAAG AAATGGCAGA CAACTTTATA AAGGAAAGCA CTGTAAAGCC AAGACCTGAC
    ATCAAAGGGC TTATTCTACC AACAGAGACA GTGAACTATG AAAGTGCCAC TATGAAAGTT
    CCTGCTCCAG TTAACCCCAG CATAACAGAA TTTAAGGTTT CATTTGACAT CTTCCCTTTC
    GACGACCCAA CTGATGATGA AGATAATGGC AGTGGGTTTG CTCATGGAAC TGACTTAGCA
    AGTTTAGCCT TGCCTGCCAG TCCTGGAAGG GCATTAATAG TGTTTTTTAG CCTTAGGGTC
    ACCAACATGA AGTTTTCCGA AGATCTCTTC AACAAGAGCT CTACTGAATA CAAGGCACTT
    GAGCAGCGAT TTCTGGAACT GCTCGTACCG TATCTACAGT CCAACCTCAG CAATTTCCAG
    AATTTAGAGA TTTTGAACTT CAGGAATGGT AGCATTGTGG TGAACAGCAG GATGAAGTTT
    GCCAAGCCAG TTCCCCATGG TGTGACAACA GCAGTGTACC TAATCTTGGA GGACTTCTGC
    AACACTGCCT ACCAAACTAT GAATCTTGCA ATAGACAAGT ATTCATTGGA TGTCGAATCA
    GGTGACCAGG CAGATCCTTG TAAGTTTCAG GCATGTAACG AGTTCTCCAA GTGTTTGGTG
    AACCGCTGGT CAGGTGAGGC CGAGTGTGTT TGTAACGCTG GTTATTTCAG CGTTGACGGA
    CTGCCATGCC AGAGCATCTG CGACATCCAA GAAGACTTCT GCCTAAATGA CGGGAAATGT
    GACATAATAC CTGGCAAGGG CGCCATCTGC AGGTGTCGCG TTGGTGAAAA CTGGTGGTAT
    CGAGGAGAAC ACTGTGAGGA ATATGTGTCA GAGCCGCTGG TGGTCGGCAT TGCCATTGCG
    TCCGTTGCTG GATTTCTCCT GGTGGCGTCG GGTGTCATCT TTTTCCTGGC CCGAACTCTG
    AGAGACCAGT ATGACACAGA TGATTCAGAA GACCCTTTAC GATATGCAGA GAACTTGCCA
    TCTCTAGAAA GAGCCACCAA GTACAACCCG ATGTTTGAAA GTGAAGTGAC TGCAGGCCAT
    AATCAGTATT ATCGGCGTTA CCCAGAAGCT CCGGCATTCA GCACGGGCAG CACCGAGATC
    TCCACTGACT TCAGCAGCGA AGAGATTCGT CACATCTATC AGAACAGTGA ACTTACTAAA
    GAGGAAATCC AGGACCGTAT TCGTATTATA GAGCTTTACG CCAAGGACCG CCAGTTTGCA
    GATTTTGTCC GACAGCACCA AGTGGCGCTT GACGCTAGAC GAGAGAGCTC TTCCAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330162.1
    CDS234..8573
    Translation

    Target ORF information:

    RefSeq Version XM_021474487.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio interphotoreceptor matrix proteoglycan 2b (impg2b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474487.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGCCTGATT TACCTTTTAA ATGCATTTGG TGGGGTTTGA CACTATTGTT GTTGACTGGA 
    TACTTTTCTA TAGATGCAGA TACTGGAACT CATGAGGATT ACTTGGCAAA TGGAAATACA
    ATGCTTTTGG ACACTAATGA AGTTTTGAAT CACTTATTGT TGTTGGACAA AGGTAAAACT
    TTGAATTACA TGTCTGCTGA AGGGAAAACA GCTCTATCTA GACATAAAAG GGACCTCCTG
    TTCCCCAATG GAGTTAAACT GTGCTCACAT GAGACGGTGC AACAGGCAGT GCAGAATCAT
    CTCAACTACT TTCACCTGCG AGTGTGCCAG GAGACAATTT GGGAGGCTTA TAAAATCTTC
    TGGGATCGCT TACCTGAGAA AGAAGAGTAC CAGATCTGGA TGAAAAAATG TCAAAATGGT
    AGCATTAGCG TGTTTGACAT CGGCCGAAGC TTTAGTCAGT CAGCTGAGCA CCTTGCTTTG
    ATTTCAAGTA GGGTAGCATC ATCCACAATA ACATCAACAA GTGCGCCCAC CAGCCCATGG
    CAACCTGAAT GCAGGCATCA GACGACGACT ACTTCGCCAA CACTAGCAAA AGCTGAACAA
    GTTGGTATTT TGATTCCTGA GGCAGTGCCA GGTTCACAAG TTGTCACACC GCAGACCTTT
    GCAGATGATA TCGTAATCAC AACTTTGCCT ACAAGGCTTC AAGTGACACC TGGAATTACC
    CCAAAGCCTG ATCTTGTCAC AGCCTTTAAG GACACAGTAA AACCTATTTC TGAAACTGAA
    CCTGAGGTCA TATTGGGTAC TACTTCTGAG AGTCCTGTTA AAGCATTGGA TACCATTACC
    ATTAAACATG AAGAGGTTGT TGTAGTCTCA GAATCTAAAT TATTGATTAC TGAGAGGACT
    TCGGAGGACT CTACTTCAAG GAAATATGAT TATGAGTTTC CCACGACCTT TAAGGACTCG
    TTAGTAATAA ACACTCCTAT AGTACTAACA ACTGAATTTT CTGAACAAAT GTATTCAGAA
    ATAGGACAAG GTTTTTCTGA TGGTACAGTT AAAACAGTAG CTGTTACTAT TCAAAAGGAA
    CTTGACATTG TCTCAAAATC TACATTATTA ACCACTGACA AAACTCCAGA AGTTTCTACT
    TCAAAAAAAT CTGAGCATGA TAATCCCACT AACTTTGTAG ATTCATTGGG AATAAGCACC
    AATACTCCTA GAGTGACCAC AAGTGAACTC AAAGAAGATC TTCATTCAGA ATTAGAACAG
    GTGGCTGGGC AGGATGTTAT TCACAGTCCT GTTAAAACAA CACATGTTAC AATTAAGCAT
    GAAGTGTTGG ACACAAATAC TACATTAATG ACAACTGACA GAACTCCATA TTCTACTTCA
    GCAAAATATG AACGTGTTAT ACCAACATCC TTTGAGGACT CACTAGTCAC AAGTTCATCT
    ACTGAACAGG ATATGACCAA TGTTCCTATT AAGACGGCAT ATGCCACAAT TTCACATGGA
    GAGTCTGAAA TTGTCACAGA GTCGACAATA ACAACCACTA ACAGAAATCT TGAAGATTCT
    TCTTTAGCAA AATATGATTA TGGGATTCCT CAAGGAATTG ATACTTCTTC GGGAGTAATT
    AATGAATCTT CTACAAAAGA ACATAATTCA GTATCTAAAT TGAATATTGG TCAGACTACT
    ACTGAAGGTC CCCAAACAAC TGATGCAGTT CAGTATGTAG AATCTGACAT TGTGTCAGAG
    GAAACGTTGA AATCCACTGG CAGAACTGCA GTTAGCTCAA ACTCTACAAA AATTGAGGCC
    GATCTTTCCA CAACTCCTGA GGACCTGACA GGAGTAAGTA CCAACACTCC TGAAATAGTC
    ACTAGAGATT CCATTACAAT ACTTATCCCA GAAACCGAGG GCATTACTAA AAATCCTGTT
    AAATCTGCAC CTATTACCAT TCAGCATGTG GATGTTGATG ATGTTTTAGC AGCTACAGTG
    ATGTCCACTC ATAAACCTCC CAAAGTCTCA CCTACAGAAA TATCCCCCGA TCAGAATCTC
    TTACCAGAGG AATCAAAGCT GCCAACTCCT ACAAAACTAG CAGAAGTTTT GTCTGCCACA
    GAAGAGTTGC CAAAGGATCA GACAGACAAG GATGTTGCTA AAATTGTATA TGTGTCTACT
    GTGCCAGAAC TGGTGTCACC AACTATTCCC GCATATACGT CAACTGACAC TACTAAGGAA
    AACCTACATG TGTTAACAGG GGGGGCAGTT GTTACATCAC TTGATAAAGA TGAGGAAGAA
    CATACACCCC ACACAAAAGA GAGCAAAATT ACACAGGTGA CAACAGGAGC AGAATTACCA
    GGTACCATAA CAGAGAGTAC TCATGGAATG TGGAGGGAAA CCCAAGAGCC AATAAATGTC
    ACACCTGATG CTGAAGACAA TCATTTGGGC AAAGAAGGGT ACATAATAAC AGAGATCCCA
    GAAGTTGCTG AAGTACCAGC TGGAGTGACA CCCAAAATGA TTGTGGAAAC TACAACAGCC
    ACTGAATCTG TGTTTACTGT GAAAGACACA TCTATAATTG TAGACAAAAC TGGCATTGTG
    AAACCTGTCG TGACTACGAC AGAGGCAGCT GAACTTACTG AGGTTGGATT CGAAGATTTA
    GGCACAGACA AAGTAACAAT TCTTGAAAAC ACACTTGAAG ATTCTGTAGG ACTAACAAAT
    GAACCTTTTG CTGTATCATG GGGTGTAACT GAAGCCACTA TTTTAGAGGA ATCATATGTG
    GAAAGTACTG AAAGTATTTC AGGTAAAGAG AAGTCCACTG AAACTATTAA GATCACAGGT
    GAAGCCACAA CTGATGACAT TGGTACTTCT GAAACTGACA TTGTAGTGCC TGTAACCGAT
    CGCATAGAAG ATAATGTAAA TGTATCACCT AAAAATGATG ATGCTAAGGA AACAAACATT
    TTTGAAGACA CAACACCAGA TCTTAGTGTT ACTAATTCTG AAAAAGACAT ACTGACAACT
    ACCATACATA AAGACATGCA TGAAGTTACA GAACCATTAA ATATTCCAGA GGTCACACAT
    AAGGTTGTGA TAGAGATCAC AGATGATGTA CGTGAGACCA GAGGAGATTC CTTACCCGAG
    ACACCAGAAG TCACAACAGA GACACCTAAG GTTGCAACAG AAGCTGATGT CAAAACAACA
    ACAAATGTTA TATCTAAAGC ACCTGAAGTT ATACTGAAGA CAACTGTAAC CAAGTCAGAT
    ACACCTGATG TCACAACTCA GACACCTTTG TTAAAGCTTG AAGATACAGA GAGGGTTGAA
    ATAGTACCTG AAACTCCTGA CATACAAGTG GTTCAAACAG AACACTCAGA AAATGATATC
    CCAACTCCTG TCTTGAAAGT GGAGGATTTC ACAGTTGCGC TGAATGACTT GACAACTCCT
    ATTCAAAAGA CTTCTTCAAC CACATTACAA CAAGACATTT CTAATGATAT TTTAGAAGAA
    AACAATGTGA TGGGAAATGA AATTGATGAT ATTGTGGTTC GACCAGTACG CCCTGTCGGC
    GATCATATTT TGGAGCTAAG TATTAAGCTA AGAGGGGAAA GCTATGACGA TGCTCTGAGG
    GATCCATCAA GTAACTACTA TCAACATCTG TCTACACTTT TTGTTGAAAA GATTGAAGAA
    GTTTTCGAAA GGCTTCCAGG TTTCAAGAAA ATATATGTTC TTGAATTCAG GCCACAGAAG
    GACATTGAAG GAGGTCTGGC AGTGGTGGTG CACTATGCTG TTGTTCTTGA GGGTGATGGG
    TCAGGAGTAA GTAATGAGAC CATGGATTAC ATAAACCTGC ATTCCAACAT GGTTGAGAAC
    TCCTTCACAG ATCCTGAGGA GCTGCCCACT GTGGTCTACA CCATCACTGA CTTCCGAAAC
    TACATCTCCG AAGCCCTTCA CAAGGATAAC TTTATCAGAA ACGCTACTCT GAATGTGGAC
    ATTGATTCTT TGCAACTTGA AAATGTGGAG ACATTGCTGC CTTCCAAACC AACCAGCAGA
    CCGTTTGACT CCAGTGACAT GATGGACAAC GTTCTTGCTT CCGAGAAACC ACCAGATGTT
    CCTGGGCAAG AATTATCCAA CAATGACATC TTCATTACAA ATTATGACAT CTTTGATCCT
    GATCCATGGA CAGGTGGACA AAGTGAAGAC AGAAATGCAA ATGATGTAAT CATCTTTGAG
    GAAAGTCCCA CTCAGTCTCC TGTTGAAGTG TCTTTGAAGA ACTTGGACAT TGAAGTTACT
    TCAAAATCTG AAACTTCAAG CAGTGCCCCT GTTCCTGATG AAGCTGTTAT TGAAGAGGAG
    GGATTTTTAC AGACTGCAAC TACAGTCAGT CCTATTATAG ACACAATAAC TGAATTTACT
    TTACCTACAG AATCACCTCA CAAGGTAGAA GAGACACCAG TTTCTCCTGT AGAACCTCCT
    GAGGTTGAGG AAACTACCCT GGGTGATTTA ACAGACCTTG GCTCAGGCTC AGGATTCTCT
    GGAGATCACA TAGGCCCTGA CATCTGGCCA TGGTCATCAG AAACATCACA AGAAGTCTTG
    AAGGAAGATA CCAATGATGA GATACACCAA CTTCAGGAAA ATCAGGAATC TATAATAAAA
    CAGCTTGAGC CTTTACATCA AGATGTGACT CCTGAGGAGC CATTCTTGGA TAGGGTTCTT
    GTGACCCAAG ACATTCGTAC CAATCCTCAT TACACAACAA CTGACCAGGC CCCTGTATTT
    TGGACAATGG AGACTTTGAC TGTTGAGCTT TCAATGCAGA CTCAAGTAGC CCCTGAACAT
    TATGATGACT ATTTTCCAGA TGCATCAATC ACTATGGCTA CACTTGTAAC CAATCAACCA
    TTGTTACATG TTCACAATCC ATTAGAAACA AAAGATCTGG ATGCATCACA AAGTCCAGGA
    ACAATAAGTT CAACTGCTGA TAAGGACTAT ACAGAACGAC TTGTTACACC CACTGTGATC
    CAACCAAGTG AAACAGTAGC TGTGGATAAG CATACTACAA CAGACAACAA ACAGAATCTG
    GAAACTATGG GTACATTATC TGCAACACTG GGCTCCACAA TGAAGTCCAC TGAAATCTCT
    TCAACTACCC AATTACCTGT GATCATCAAC AATAATACAG TTTCAGTGGA AGGTCCTGCC
    AATTCTTCAG AAGCTGTTCA GCATTCTGTA AATGAAGGAA TCACAGAGTT ACCTGCTTTG
    GTCTGGCCAG ATATTGAAGG ATCAGATGAA GAATTCAAAG TTTTAGATGA GGACATGGAA
    CTCGTCAAGT TTGTGCCTAC AAAAAGCCCA GTGACTGAAC TCTTAGAAGA AGATTTAGTT
    GGTGATGAAA TATTGGTTGC AACAACAACA ACAACAACAA CTCTATCCTC AACAACAGTA
    GCGACTGTGG TAACAAGTTT AGGTCATTCA GCCTCCCTGA CTCCTGAAAA GGACTCACCT
    TTCACTTGGA TATCACATTC ATCAATAGAT GAGGATGAAC CTGTGCAAGA GTCAACCACA
    GAACCTCTAC CCACCCAATC ACCCACTGCT TCTGTTCTTC CAAAGACAAC TACACAGACA
    TTGATGCACA CATACATAGC AGCTGTTGCA GGGGAGCCTG TTTTATCAGA TAACATGGAT
    AATAAGGACT CAACCACAAT GTCCACCCTT TTAGATGTTT CAAAACATTT AAAGACCCTT
    AGTTCCACCT CTGTACAATC ATTTTCAGAA CCAACATTTA CACCTACTGT CCACATAATT
    AAGCATGGAG AAACTTCAGA TGACTCAGTA CAAGATGTAG TTGAGACTGG AGAAGTTTTA
    GATGACTCTG TACAAGATGT ACTTGAGCAT GAAGACATTT CATATGGCTC TGTACAAGAT
    GTAGTTGTAT CTGGAGAAAC TTCAAATGAC TCAGTACAAG ATGTAGTTGA GACTGGAGAA
    GTTTTAGATG ATTCTGTACA AGATGTACTT GAGCATGAGG ACATTTTATC TGGCCCAGTT
    CAAGATGTAG TTGTGTCTGG AGAAACTGCA GATGACTCAG TACAAGATTT AGTTGAGACT
    GGAGAAGTTT TAGATGATTC CGTACAAGAT GTACTTGAGC ATGAAGTAAT TTCATTTGGC
    CCAGTACAAG ATGTAGTTGT ATCTGGAAAA ACTTCAAATG ACTCAGTACA AGATGTAGTT
    GAGAGTGCAA AAGATTCAGA TGCCCCAATA CAAGATGTAT TTGAGCATAA AGACATTTCA
    GTTGACTCTG TACAAGATGT AGTTGTGTCT GGAGAAACTG CAGATGACTC TATTCAAGAT
    TTATTTAAGC ATGAAGGCAC TTCGTTCGAC ACAGTACAAG ATGAGGTTGA GTCTAGAGAA
    ACTTCAGATG ACTCAGTACA AAATGTAGTT GAGCATGAAG ACTCTTCAGA TGACGCAGTT
    CAAGATGTAG TCGAGTCTGG AGACACTTCA GATGACTCTG TACAAGATAT ATTTGAGTCT
    GCAGAAACTT CAGATGACTC TGTACAAGAT GTAGCTGAGA CTGGAAAAGA ATCAGATGAC
    TTAGTACAAG ATATATTTGA GAATAAAGAC ATTTCAGTTG ACTCAGTACA AGATGTAGTT
    GTATCTGGAG AGACTTCAGA TGACTCTATT CAAGGTTTAC TTGAGCATGA AGGCACTTCA
    GTCGACATAG TACAAGATGA GGTTGAGTTT AGAGAAAATT CAGATGACTC AGTACTAAAT
    GTAGTCAAGC ATGAAGATAC TTCAGATGAC TCAATACAAG ATGTAGTCGA GACAGGAGAA
    GTTTCAGATA GCTCAGTACA AGATGTAGCT GAGTCTGGAG AAACTTTAGA TGACTCTGTT
    CAAGATTTTG TTAAAACTGG AGAAATTTCC ATTGACTCAG TACACAATTT ATTTGAGCCT
    GAAAAAAACG TAGATGACTC TGTACAAGAT GTAGTTGAGA CTGGAGAAGT TTCTGTTGAT
    TCAGTACAAG ATGTAATTGA GCTTGGAGAC ACTTTAGATG ACTCTGCACA AGGAATAGTT
    GAACCTGAAG AAATGGCAGA CAACTTTATA AAGGAAAGCA CTGTAAAGCC AAGACCTGAC
    ATCAAAGGGC TTATTCTACC AACAGAGACA GTGAACTATG AAAGTGCCAC TATGAAAGTT
    CCTGCTCCAG TTAACCCCAG CATAACAGAA TTTAAGGTTT CATTTGACAT CTTCCCTTTC
    GACGACCCAA CTGATGATGA AGATAATGGC AGTGGGTTTG CTCATGGAAC TGACTTAGCA
    AGTTTAGCCT TGCCTGCCAG TCCTGGAAGG GCATTAATAG TGTTTTTTAG CCTTAGGGTC
    ACCAACATGA AGTTTTCCGA AGATCTCTTC AACAAGAGCT CTACTGAATA CAAGGCACTT
    GAGCAGCGAT TTCTGGAACT GCTCGTACCG TATCTACAGT CCAACCTCAG CAATTTCCAG
    AATTTAGAGA TTTTGAACTT CAGGAATGGT AGCATTGTGG TGAACAGCAG GATGAAGTTT
    GCCAAGCCAG TTCCCCATGG TGTGACAACA GCAGTGTACC TAATCTTGGA GGACTTCTGC
    AACACTGCCT ACCAAACTAT GAATCTTGCA ATAGACAAGT ATTCATTGGA TGTCGAATCA
    GGTGACCAGG CAGATCCTTG TAAGTTTCAG GCATGTAACG AGTTCTCCAA GTGTTTGGTG
    AACCGCTGGT CAGGTGAGGC CGAGTGTGTT TGTAACGCTG GTTATTTCAG CGTTGACGGA
    CTGCCATGCC AGAGCATCTG CGACATCCAA GAAGACTTCT GCCTAAATGA CGGGAAATGT
    GACATAATAC CTGGCAAGGG CGCCATCTGC AGGTGTCGCG TTGGTGAAAA CTGGTGGTAT
    CGAGGAGAAC ACTGTGAGGA ATATGTGTCA GAGCCGCTGG TGGTCGGCAT TGCCATTGCG
    TCCGTTGCTG GATTTCTCCT GGTGGCGTCG GGTGTCATCT TTTTCCTGGC CCGAACTCTG
    AGAGACCAGT ATGACACAGA TGATTCAGAA GACCCTTTAC GATATGCAGA GAACTTGCCA
    TCTCTAGAAA GAGCCACCAA GTACAACCCG ATGTTTGAAA GTGAAGTGAC TGCAGGCCAT
    AATCAGTATT ATCGGCGTTA CCCAGAAGCT CCGGCATTCA GCACGGGCAG CACCGAGATC
    TCCACTGACT TCAGCAGCGA AGAGATTCGT CACATCTATC AGAACAGTGA ACTTACTAAA
    GAGGAAATCC AGGACCGTAT TCGTATTATA GAGCTTTACG CCAAGGACCG CCAGTTTGCA
    GATTTTGTCC GACAGCACCA AGTGGCGCTT GACGCTAGAC GAGAGAGCTC TTCCAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146187
    Clone ID Related Accession (Same CDS sequence) XM_021474487.1 , XM_021469613.1 , XM_021473523.1
    Accession Version XM_021469613.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8340bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin homolog isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
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    3601
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    3721
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    4141
    4201
    4261
    4321
    4381
    4441
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    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
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    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
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    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
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    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGCCTGATT TACCTTTTAA ATGCATTTGG TGGGGTTTGA CACTATTGTT GTTGACTGGA 
    TACTTTTCTA TAGATGCAGA TACTGGAACT CATGAGGATT ACTTGGCAAA TGGAAATACA
    ATGCTTTTGG ACACTAATGA AGTTTTGAAT CACTTATTGT TGTTGGACAA AGGTAAAACT
    TTGAATTACA TGTCTGCTGA AGGGAAAACA GCTCTATCTA GACATAAAAG GGACCTCCTG
    TTCCCCAATG GAGTTAAACT GTGCTCACAT GAGACGGTGC AACAGGCAGT GCAGAATCAT
    CTCAACTACT TTCACCTGCG AGTGTGCCAG GAGACAATTT GGGAGGCTTA TAAAATCTTC
    TGGGATCGCT TACCTGAGAA AGAAGAGTAC CAGATCTGGA TGAAAAAATG TCAAAATGGT
    AGCATTAGCG TGTTTGACAT CGGCCGAAGC TTTAGTCAGT CAGCTGAGCA CCTTGCTTTG
    ATTTCAAGTA GGGTAGCATC ATCCACAATA ACATCAACAA GTGCGCCCAC CAGCCCATGG
    CAACCTGAAT GCAGGCATCA GACGACGACT ACTTCGCCAA CACTAGCAAA AGCTGAACAA
    GTTGGTATTT TGATTCCTGA GGCAGTGCCA GGTTCACAAG TTGTCACACC GCAGACCTTT
    GCAGATGATA TCGTAATCAC AACTTTGCCT ACAAGGCTTC AAGTGACACC TGGAATTACC
    CCAAAGCCTG ATCTTGTCAC AGCCTTTAAG GACACAGTAA AACCTATTTC TGAAACTGAA
    CCTGAGGTCA TATTGGGTAC TACTTCTGAG AGTCCTGTTA AAGCATTGGA TACCATTACC
    ATTAAACATG AAGAGGTTGT TGTAGTCTCA GAATCTAAAT TATTGATTAC TGAGAGGACT
    TCGGAGGACT CTACTTCAAG GAAATATGAT TATGAGTTTC CCACGACCTT TAAGGACTCG
    TTAGTAATAA ACACTCCTAT AGTACTAACA ACTGAATTTT CTGAACAAAT GTATTCAGAA
    ATAGGACAAG GTTTTTCTGA TGGTACAGTT AAAACAGTAG CTGTTACTAT TCAAAAGGAA
    CTTGACATTG TCTCAAAATC TACATTATTA ACCACTGACA AAACTCCAGA AGTTTCTACT
    TCAAAAAAAT CTGAGCATGA TAATCCCACT AACTTTGTAG ATTCATTGGG AATAAGCACC
    AATACTCCTA GAGTGACCAC AAGTGAACTC AAAGAAGATC TTCATTCAGA ATTAGAACAG
    GTGGCTGGGC AGGATGTTAT TCACAGTCCT GTTAAAACAA CACATGTTAC AATTAAGCAT
    GAAGTGTTGG ACACAAATAC TACATTAATG ACAACTGACA GAACTCCATA TTCTACTTCA
    GCAAAATATG AACGTGTTAT ACCAACATCC TTTGAGGACT CACTAGTCAC AAGTTCATCT
    ACTGAACAGG ATATGACCAA TGTTCCTATT AAGACGGCAT ATGCCACAAT TTCACATGGA
    GAGTCTGAAA TTGTCACAGA GTCGACAATA ACAACCACTA ACAGAAATCT TGAAGATTCT
    TCTTTAGCAA AATATGATTA TGGGATTCCT CAAGGAATTG ATACTTCTTC GGGAGTAATT
    AATGAATCTT CTACAAAAGA ACATAATTCA GTATCTAAAT TGAATATTGG TCAGACTACT
    ACTGAAGGTC CCCAAACAAC TGATGCAGTT CAGTATGTAG AATCTGACAT TGTGTCAGAG
    GAAACGTTGA AATCCACTGG CAGAACTGCA GTTAGCTCAA ACTCTACAAA AATTGAGGCC
    GATCTTTCCA CAACTCCTGA GGACCTGACA GGAGTAAGTA CCAACACTCC TGAAATAGTC
    ACTAGAGATT CCATTACAAT ACTTATCCCA GAAACCGAGG GCATTACTAA AAATCCTGTT
    AAATCTGCAC CTATTACCAT TCAGCATGTG GATGTTGATG ATGTTTTAGC AGCTACAGTG
    ATGTCCACTC ATAAACCTCC CAAAGTCTCA CCTACAGAAA TATCCCCCGA TCAGAATCTC
    TTACCAGAGG AATCAAAGCT GCCAACTCCT ACAAAACTAG CAGAAGTTTT GTCTGCCACA
    GAAGAGTTGC CAAAGGATCA GACAGACAAG GATGTTGCTA AAATTGTATA TGTGTCTACT
    GTGCCAGAAC TGGTGTCACC AACTATTCCC GCATATACGT CAACTGACAC TACTAAGGAA
    AACCTACATG TGTTAACAGG GGGGGCAGTT GTTACATCAC TTGATAAAGA TGAGGAAGAA
    CATACACCCC ACACAAAAGA GAGCAAAATT ACACAGGTGA CAACAGGAGC AGAATTACCA
    GGTACCATAA CAGAGAGTAC TCATGGAATG TGGAGGGAAA CCCAAGAGCC AATAAATGTC
    ACACCTGATG CTGAAGACAA TCATTTGGGC AAAGAAGGGT ACATAATAAC AGAGATCCCA
    GAAGTTGCTG AAGTACCAGC TGGAGTGACA CCCAAAATGA TTGTGGAAAC TACAACAGCC
    ACTGAATCTG TGTTTACTGT GAAAGACACA TCTATAATTG TAGACAAAAC TGGCATTGTG
    AAACCTGTCG TGACTACGAC AGAGGCAGCT GAACTTACTG AGGTTGGATT CGAAGATTTA
    GGCACAGACA AAGTAACAAT TCTTGAAAAC ACACTTGAAG ATTCTGTAGG ACTAACAAAT
    GAACCTTTTG CTGTATCATG GGGTGTAACT GAAGCCACTA TTTTAGAGGA ATCATATGTG
    GAAAGTACTG AAAGTATTTC AGGTAAAGAG AAGTCCACTG AAACTATTAA GATCACAGGT
    GAAGCCACAA CTGATGACAT TGGTACTTCT GAAACTGACA TTGTAGTGCC TGTAACCGAT
    CGCATAGAAG ATAATGTAAA TGTATCACCT AAAAATGATG ATGCTAAGGA AACAAACATT
    TTTGAAGACA CAACACCAGA TCTTAGTGTT ACTAATTCTG AAAAAGACAT ACTGACAACT
    ACCATACATA AAGACATGCA TGAAGTTACA GAACCATTAA ATATTCCAGA GGTCACACAT
    AAGGTTGTGA TAGAGATCAC AGATGATGTA CGTGAGACCA GAGGAGATTC CTTACCCGAG
    ACACCAGAAG TCACAACAGA GACACCTAAG GTTGCAACAG AAGCTGATGT CAAAACAACA
    ACAAATGTTA TATCTAAAGC ACCTGAAGTT ATACTGAAGA CAACTGTAAC CAAGTCAGAT
    ACACCTGATG TCACAACTCA GACACCTTTG TTAAAGCTTG AAGATACAGA GAGGGTTGAA
    ATAGTACCTG AAACTCCTGA CATACAAGTG GTTCAAACAG AACACTCAGA AAATGATATC
    CCAACTCCTG TCTTGAAAGT GGAGGATTTC ACAGTTGCGC TGAATGACTT GACAACTCCT
    ATTCAAAAGA CTTCTTCAAC CACATTACAA CAAGACATTT CTAATGATAT TTTAGAAGAA
    AACAATGTGA TGGGAAATGA AATTGATGAT ATTGTGGTTC GACCAGTACG CCCTGTCGGC
    GATCATATTT TGGAGCTAAG TATTAAGCTA AGAGGGGAAA GCTATGACGA TGCTCTGAGG
    GATCCATCAA GTAACTACTA TCAACATCTG TCTACACTTT TTGTTGAAAA GATTGAAGAA
    GTTTTCGAAA GGCTTCCAGG TTTCAAGAAA ATATATGTTC TTGAATTCAG GCCACAGAAG
    GACATTGAAG GAGGTCTGGC AGTGGTGGTG CACTATGCTG TTGTTCTTGA GGGTGATGGG
    TCAGGAGTAA GTAATGAGAC CATGGATTAC ATAAACCTGC ATTCCAACAT GGTTGAGAAC
    TCCTTCACAG ATCCTGAGGA GCTGCCCACT GTGGTCTACA CCATCACTGA CTTCCGAAAC
    TACATCTCCG AAGCCCTTCA CAAGGATAAC TTTATCAGAA ACGCTACTCT GAATGTGGAC
    ATTGATTCTT TGCAACTTGA AAATGTGGAG ACATTGCTGC CTTCCAAACC AACCAGCAGA
    CCGTTTGACT CCAGTGACAT GATGGACAAC GTTCTTGCTT CCGAGAAACC ACCAGATGTT
    CCTGGGCAAG AATTATCCAA CAATGACATC TTCATTACAA ATTATGACAT CTTTGATCCT
    GATCCATGGA CAGGTGGACA AAGTGAAGAC AGAAATGCAA ATGATGTAAT CATCTTTGAG
    GAAAGTCCCA CTCAGTCTCC TGTTGAAGTG TCTTTGAAGA ACTTGGACAT TGAAGTTACT
    TCAAAATCTG AAACTTCAAG CAGTGCCCCT GTTCCTGATG AAGCTGTTAT TGAAGAGGAG
    GGATTTTTAC AGACTGCAAC TACAGTCAGT CCTATTATAG ACACAATAAC TGAATTTACT
    TTACCTACAG AATCACCTCA CAAGGTAGAA GAGACACCAG TTTCTCCTGT AGAACCTCCT
    GAGGTTGAGG AAACTACCCT GGGTGATTTA ACAGACCTTG GCTCAGGCTC AGGATTCTCT
    GGAGATCACA TAGGCCCTGA CATCTGGCCA TGGTCATCAG AAACATCACA AGAAGTCTTG
    AAGGAAGATA CCAATGATGA GATACACCAA CTTCAGGAAA ATCAGGAATC TATAATAAAA
    CAGCTTGAGC CTTTACATCA AGATGTGACT CCTGAGGAGC CATTCTTGGA TAGGGTTCTT
    GTGACCCAAG ACATTCGTAC CAATCCTCAT TACACAACAA CTGACCAGGC CCCTGTATTT
    TGGACAATGG AGACTTTGAC TGTTGAGCTT TCAATGCAGA CTCAAGTAGC CCCTGAACAT
    TATGATGACT ATTTTCCAGA TGCATCAATC ACTATGGCTA CACTTGTAAC CAATCAACCA
    TTGTTACATG TTCACAATCC ATTAGAAACA AAAGATCTGG ATGCATCACA AAGTCCAGGA
    ACAATAAGTT CAACTGCTGA TAAGGACTAT ACAGAACGAC TTGTTACACC CACTGTGATC
    CAACCAAGTG AAACAGTAGC TGTGGATAAG CATACTACAA CAGACAACAA ACAGAATCTG
    GAAACTATGG GTACATTATC TGCAACACTG GGCTCCACAA TGAAGTCCAC TGAAATCTCT
    TCAACTACCC AATTACCTGT GATCATCAAC AATAATACAG TTTCAGTGGA AGGTCCTGCC
    AATTCTTCAG AAGCTGTTCA GCATTCTGTA AATGAAGGAA TCACAGAGTT ACCTGCTTTG
    GTCTGGCCAG ATATTGAAGG ATCAGATGAA GAATTCAAAG TTTTAGATGA GGACATGGAA
    CTCGTCAAGT TTGTGCCTAC AAAAAGCCCA GTGACTGAAC TCTTAGAAGA AGATTTAGTT
    GGTGATGAAA TATTGGTTGC AACAACAACA ACAACAACAA CTCTATCCTC AACAACAGTA
    GCGACTGTGG TAACAAGTTT AGGTCATTCA GCCTCCCTGA CTCCTGAAAA GGACTCACCT
    TTCACTTGGA TATCACATTC ATCAATAGAT GAGGATGAAC CTGTGCAAGA GTCAACCACA
    GAACCTCTAC CCACCCAATC ACCCACTGCT TCTGTTCTTC CAAAGACAAC TACACAGACA
    TTGATGCACA CATACATAGC AGCTGTTGCA GGGGAGCCTG TTTTATCAGA TAACATGGAT
    AATAAGGACT CAACCACAAT GTCCACCCTT TTAGATGTTT CAAAACATTT AAAGACCCTT
    AGTTCCACCT CTGTACAATC ATTTTCAGAA CCAACATTTA CACCTACTGT CCACATAATT
    AAGCATGGAG AAACTTCAGA TGACTCAGTA CAAGATGTAG TTGAGACTGG AGAAGTTTTA
    GATGACTCTG TACAAGATGT ACTTGAGCAT GAAGACATTT CATATGGCTC TGTACAAGAT
    GTAGTTGTAT CTGGAGAAAC TTCAAATGAC TCAGTACAAG ATGTAGTTGA GACTGGAGAA
    GTTTTAGATG ATTCTGTACA AGATGTACTT GAGCATGAGG ACATTTTATC TGGCCCAGTT
    CAAGATGTAG TTGTGTCTGG AGAAACTGCA GATGACTCAG TACAAGATTT AGTTGAGACT
    GGAGAAGTTT TAGATGATTC CGTACAAGAT GTACTTGAGC ATGAAGTAAT TTCATTTGGC
    CCAGTACAAG ATGTAGTTGT ATCTGGAAAA ACTTCAAATG ACTCAGTACA AGATGTAGTT
    GAGAGTGCAA AAGATTCAGA TGCCCCAATA CAAGATGTAT TTGAGCATAA AGACATTTCA
    GTTGACTCTG TACAAGATGT AGTTGTGTCT GGAGAAACTG CAGATGACTC TATTCAAGAT
    TTATTTAAGC ATGAAGGCAC TTCGTTCGAC ACAGTACAAG ATGAGGTTGA GTCTAGAGAA
    ACTTCAGATG ACTCAGTACA AAATGTAGTT GAGCATGAAG ACTCTTCAGA TGACGCAGTT
    CAAGATGTAG TCGAGTCTGG AGACACTTCA GATGACTCTG TACAAGATAT ATTTGAGTCT
    GCAGAAACTT CAGATGACTC TGTACAAGAT GTAGCTGAGA CTGGAAAAGA ATCAGATGAC
    TTAGTACAAG ATATATTTGA GAATAAAGAC ATTTCAGTTG ACTCAGTACA AGATGTAGTT
    GTATCTGGAG AGACTTCAGA TGACTCTATT CAAGGTTTAC TTGAGCATGA AGGCACTTCA
    GTCGACATAG TACAAGATGA GGTTGAGTTT AGAGAAAATT CAGATGACTC AGTACTAAAT
    GTAGTCAAGC ATGAAGATAC TTCAGATGAC TCAATACAAG ATGTAGTCGA GACAGGAGAA
    GTTTCAGATA GCTCAGTACA AGATGTAGCT GAGTCTGGAG AAACTTTAGA TGACTCTGTT
    CAAGATTTTG TTAAAACTGG AGAAATTTCC ATTGACTCAG TACACAATTT ATTTGAGCCT
    GAAAAAAACG TAGATGACTC TGTACAAGAT GTAGTTGAGA CTGGAGAAGT TTCTGTTGAT
    TCAGTACAAG ATGTAATTGA GCTTGGAGAC ACTTTAGATG ACTCTGCACA AGGAATAGTT
    GAACCTGAAG AAATGGCAGA CAACTTTATA AAGGAAAGCA CTGTAAAGCC AAGACCTGAC
    ATCAAAGGGC TTATTCTACC AACAGAGACA GTGAACTATG AAAGTGCCAC TATGAAAGTT
    CCTGCTCCAG TTAACCCCAG CATAACAGAA TTTAAGGTTT CATTTGACAT CTTCCCTTTC
    GACGACCCAA CTGATGATGA AGATAATGGC AGTGGGTTTG CTCATGGAAC TGACTTAGCA
    AGTTTAGCCT TGCCTGCCAG TCCTGGAAGG GCATTAATAG TGTTTTTTAG CCTTAGGGTC
    ACCAACATGA AGTTTTCCGA AGATCTCTTC AACAAGAGCT CTACTGAATA CAAGGCACTT
    GAGCAGCGAT TTCTGGAACT GCTCGTACCG TATCTACAGT CCAACCTCAG CAATTTCCAG
    AATTTAGAGA TTTTGAACTT CAGGAATGGT AGCATTGTGG TGAACAGCAG GATGAAGTTT
    GCCAAGCCAG TTCCCCATGG TGTGACAACA GCAGTGTACC TAATCTTGGA GGACTTCTGC
    AACACTGCCT ACCAAACTAT GAATCTTGCA ATAGACAAGT ATTCATTGGA TGTCGAATCA
    GGTGACCAGG CAGATCCTTG TAAGTTTCAG GCATGTAACG AGTTCTCCAA GTGTTTGGTG
    AACCGCTGGT CAGGTGAGGC CGAGTGTGTT TGTAACGCTG GTTATTTCAG CGTTGACGGA
    CTGCCATGCC AGAGCATCTG CGACATCCAA GAAGACTTCT GCCTAAATGA CGGGAAATGT
    GACATAATAC CTGGCAAGGG CGCCATCTGC AGGTGTCGCG TTGGTGAAAA CTGGTGGTAT
    CGAGGAGAAC ACTGTGAGGA ATATGTGTCA GAGCCGCTGG TGGTCGGCAT TGCCATTGCG
    TCCGTTGCTG GATTTCTCCT GGTGGCGTCG GGTGTCATCT TTTTCCTGGC CCGAACTCTG
    AGAGACCAGT ATGACACAGA TGATTCAGAA GACCCTTTAC GATATGCAGA GAACTTGCCA
    TCTCTAGAAA GAGCCACCAA GTACAACCCG ATGTTTGAAA GTGAAGTGAC TGCAGGCCAT
    AATCAGTATT ATCGGCGTTA CCCAGAAGCT CCGGCATTCA GCACGGGCAG CACCGAGATC
    TCCACTGACT TCAGCAGCGA AGAGATTCGT CACATCTATC AGAACAGTGA ACTTACTAAA
    GAGGAAATCC AGGACCGTAT TCGTATTATA GAGCTTTACG CCAAGGACCG CCAGTTTGCA
    GATTTTGTCC GACAGCACCA AGTGGCGCTT GACGCTAGAC GAGAGAGCTC TTCCAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325288.1
    CDS234..8573
    Translation

    Target ORF information:

    RefSeq Version XM_021469613.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio interphotoreceptor matrix proteoglycan 2b (impg2b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469613.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGCCTGATT TACCTTTTAA ATGCATTTGG TGGGGTTTGA CACTATTGTT GTTGACTGGA 
    TACTTTTCTA TAGATGCAGA TACTGGAACT CATGAGGATT ACTTGGCAAA TGGAAATACA
    ATGCTTTTGG ACACTAATGA AGTTTTGAAT CACTTATTGT TGTTGGACAA AGGTAAAACT
    TTGAATTACA TGTCTGCTGA AGGGAAAACA GCTCTATCTA GACATAAAAG GGACCTCCTG
    TTCCCCAATG GAGTTAAACT GTGCTCACAT GAGACGGTGC AACAGGCAGT GCAGAATCAT
    CTCAACTACT TTCACCTGCG AGTGTGCCAG GAGACAATTT GGGAGGCTTA TAAAATCTTC
    TGGGATCGCT TACCTGAGAA AGAAGAGTAC CAGATCTGGA TGAAAAAATG TCAAAATGGT
    AGCATTAGCG TGTTTGACAT CGGCCGAAGC TTTAGTCAGT CAGCTGAGCA CCTTGCTTTG
    ATTTCAAGTA GGGTAGCATC ATCCACAATA ACATCAACAA GTGCGCCCAC CAGCCCATGG
    CAACCTGAAT GCAGGCATCA GACGACGACT ACTTCGCCAA CACTAGCAAA AGCTGAACAA
    GTTGGTATTT TGATTCCTGA GGCAGTGCCA GGTTCACAAG TTGTCACACC GCAGACCTTT
    GCAGATGATA TCGTAATCAC AACTTTGCCT ACAAGGCTTC AAGTGACACC TGGAATTACC
    CCAAAGCCTG ATCTTGTCAC AGCCTTTAAG GACACAGTAA AACCTATTTC TGAAACTGAA
    CCTGAGGTCA TATTGGGTAC TACTTCTGAG AGTCCTGTTA AAGCATTGGA TACCATTACC
    ATTAAACATG AAGAGGTTGT TGTAGTCTCA GAATCTAAAT TATTGATTAC TGAGAGGACT
    TCGGAGGACT CTACTTCAAG GAAATATGAT TATGAGTTTC CCACGACCTT TAAGGACTCG
    TTAGTAATAA ACACTCCTAT AGTACTAACA ACTGAATTTT CTGAACAAAT GTATTCAGAA
    ATAGGACAAG GTTTTTCTGA TGGTACAGTT AAAACAGTAG CTGTTACTAT TCAAAAGGAA
    CTTGACATTG TCTCAAAATC TACATTATTA ACCACTGACA AAACTCCAGA AGTTTCTACT
    TCAAAAAAAT CTGAGCATGA TAATCCCACT AACTTTGTAG ATTCATTGGG AATAAGCACC
    AATACTCCTA GAGTGACCAC AAGTGAACTC AAAGAAGATC TTCATTCAGA ATTAGAACAG
    GTGGCTGGGC AGGATGTTAT TCACAGTCCT GTTAAAACAA CACATGTTAC AATTAAGCAT
    GAAGTGTTGG ACACAAATAC TACATTAATG ACAACTGACA GAACTCCATA TTCTACTTCA
    GCAAAATATG AACGTGTTAT ACCAACATCC TTTGAGGACT CACTAGTCAC AAGTTCATCT
    ACTGAACAGG ATATGACCAA TGTTCCTATT AAGACGGCAT ATGCCACAAT TTCACATGGA
    GAGTCTGAAA TTGTCACAGA GTCGACAATA ACAACCACTA ACAGAAATCT TGAAGATTCT
    TCTTTAGCAA AATATGATTA TGGGATTCCT CAAGGAATTG ATACTTCTTC GGGAGTAATT
    AATGAATCTT CTACAAAAGA ACATAATTCA GTATCTAAAT TGAATATTGG TCAGACTACT
    ACTGAAGGTC CCCAAACAAC TGATGCAGTT CAGTATGTAG AATCTGACAT TGTGTCAGAG
    GAAACGTTGA AATCCACTGG CAGAACTGCA GTTAGCTCAA ACTCTACAAA AATTGAGGCC
    GATCTTTCCA CAACTCCTGA GGACCTGACA GGAGTAAGTA CCAACACTCC TGAAATAGTC
    ACTAGAGATT CCATTACAAT ACTTATCCCA GAAACCGAGG GCATTACTAA AAATCCTGTT
    AAATCTGCAC CTATTACCAT TCAGCATGTG GATGTTGATG ATGTTTTAGC AGCTACAGTG
    ATGTCCACTC ATAAACCTCC CAAAGTCTCA CCTACAGAAA TATCCCCCGA TCAGAATCTC
    TTACCAGAGG AATCAAAGCT GCCAACTCCT ACAAAACTAG CAGAAGTTTT GTCTGCCACA
    GAAGAGTTGC CAAAGGATCA GACAGACAAG GATGTTGCTA AAATTGTATA TGTGTCTACT
    GTGCCAGAAC TGGTGTCACC AACTATTCCC GCATATACGT CAACTGACAC TACTAAGGAA
    AACCTACATG TGTTAACAGG GGGGGCAGTT GTTACATCAC TTGATAAAGA TGAGGAAGAA
    CATACACCCC ACACAAAAGA GAGCAAAATT ACACAGGTGA CAACAGGAGC AGAATTACCA
    GGTACCATAA CAGAGAGTAC TCATGGAATG TGGAGGGAAA CCCAAGAGCC AATAAATGTC
    ACACCTGATG CTGAAGACAA TCATTTGGGC AAAGAAGGGT ACATAATAAC AGAGATCCCA
    GAAGTTGCTG AAGTACCAGC TGGAGTGACA CCCAAAATGA TTGTGGAAAC TACAACAGCC
    ACTGAATCTG TGTTTACTGT GAAAGACACA TCTATAATTG TAGACAAAAC TGGCATTGTG
    AAACCTGTCG TGACTACGAC AGAGGCAGCT GAACTTACTG AGGTTGGATT CGAAGATTTA
    GGCACAGACA AAGTAACAAT TCTTGAAAAC ACACTTGAAG ATTCTGTAGG ACTAACAAAT
    GAACCTTTTG CTGTATCATG GGGTGTAACT GAAGCCACTA TTTTAGAGGA ATCATATGTG
    GAAAGTACTG AAAGTATTTC AGGTAAAGAG AAGTCCACTG AAACTATTAA GATCACAGGT
    GAAGCCACAA CTGATGACAT TGGTACTTCT GAAACTGACA TTGTAGTGCC TGTAACCGAT
    CGCATAGAAG ATAATGTAAA TGTATCACCT AAAAATGATG ATGCTAAGGA AACAAACATT
    TTTGAAGACA CAACACCAGA TCTTAGTGTT ACTAATTCTG AAAAAGACAT ACTGACAACT
    ACCATACATA AAGACATGCA TGAAGTTACA GAACCATTAA ATATTCCAGA GGTCACACAT
    AAGGTTGTGA TAGAGATCAC AGATGATGTA CGTGAGACCA GAGGAGATTC CTTACCCGAG
    ACACCAGAAG TCACAACAGA GACACCTAAG GTTGCAACAG AAGCTGATGT CAAAACAACA
    ACAAATGTTA TATCTAAAGC ACCTGAAGTT ATACTGAAGA CAACTGTAAC CAAGTCAGAT
    ACACCTGATG TCACAACTCA GACACCTTTG TTAAAGCTTG AAGATACAGA GAGGGTTGAA
    ATAGTACCTG AAACTCCTGA CATACAAGTG GTTCAAACAG AACACTCAGA AAATGATATC
    CCAACTCCTG TCTTGAAAGT GGAGGATTTC ACAGTTGCGC TGAATGACTT GACAACTCCT
    ATTCAAAAGA CTTCTTCAAC CACATTACAA CAAGACATTT CTAATGATAT TTTAGAAGAA
    AACAATGTGA TGGGAAATGA AATTGATGAT ATTGTGGTTC GACCAGTACG CCCTGTCGGC
    GATCATATTT TGGAGCTAAG TATTAAGCTA AGAGGGGAAA GCTATGACGA TGCTCTGAGG
    GATCCATCAA GTAACTACTA TCAACATCTG TCTACACTTT TTGTTGAAAA GATTGAAGAA
    GTTTTCGAAA GGCTTCCAGG TTTCAAGAAA ATATATGTTC TTGAATTCAG GCCACAGAAG
    GACATTGAAG GAGGTCTGGC AGTGGTGGTG CACTATGCTG TTGTTCTTGA GGGTGATGGG
    TCAGGAGTAA GTAATGAGAC CATGGATTAC ATAAACCTGC ATTCCAACAT GGTTGAGAAC
    TCCTTCACAG ATCCTGAGGA GCTGCCCACT GTGGTCTACA CCATCACTGA CTTCCGAAAC
    TACATCTCCG AAGCCCTTCA CAAGGATAAC TTTATCAGAA ACGCTACTCT GAATGTGGAC
    ATTGATTCTT TGCAACTTGA AAATGTGGAG ACATTGCTGC CTTCCAAACC AACCAGCAGA
    CCGTTTGACT CCAGTGACAT GATGGACAAC GTTCTTGCTT CCGAGAAACC ACCAGATGTT
    CCTGGGCAAG AATTATCCAA CAATGACATC TTCATTACAA ATTATGACAT CTTTGATCCT
    GATCCATGGA CAGGTGGACA AAGTGAAGAC AGAAATGCAA ATGATGTAAT CATCTTTGAG
    GAAAGTCCCA CTCAGTCTCC TGTTGAAGTG TCTTTGAAGA ACTTGGACAT TGAAGTTACT
    TCAAAATCTG AAACTTCAAG CAGTGCCCCT GTTCCTGATG AAGCTGTTAT TGAAGAGGAG
    GGATTTTTAC AGACTGCAAC TACAGTCAGT CCTATTATAG ACACAATAAC TGAATTTACT
    TTACCTACAG AATCACCTCA CAAGGTAGAA GAGACACCAG TTTCTCCTGT AGAACCTCCT
    GAGGTTGAGG AAACTACCCT GGGTGATTTA ACAGACCTTG GCTCAGGCTC AGGATTCTCT
    GGAGATCACA TAGGCCCTGA CATCTGGCCA TGGTCATCAG AAACATCACA AGAAGTCTTG
    AAGGAAGATA CCAATGATGA GATACACCAA CTTCAGGAAA ATCAGGAATC TATAATAAAA
    CAGCTTGAGC CTTTACATCA AGATGTGACT CCTGAGGAGC CATTCTTGGA TAGGGTTCTT
    GTGACCCAAG ACATTCGTAC CAATCCTCAT TACACAACAA CTGACCAGGC CCCTGTATTT
    TGGACAATGG AGACTTTGAC TGTTGAGCTT TCAATGCAGA CTCAAGTAGC CCCTGAACAT
    TATGATGACT ATTTTCCAGA TGCATCAATC ACTATGGCTA CACTTGTAAC CAATCAACCA
    TTGTTACATG TTCACAATCC ATTAGAAACA AAAGATCTGG ATGCATCACA AAGTCCAGGA
    ACAATAAGTT CAACTGCTGA TAAGGACTAT ACAGAACGAC TTGTTACACC CACTGTGATC
    CAACCAAGTG AAACAGTAGC TGTGGATAAG CATACTACAA CAGACAACAA ACAGAATCTG
    GAAACTATGG GTACATTATC TGCAACACTG GGCTCCACAA TGAAGTCCAC TGAAATCTCT
    TCAACTACCC AATTACCTGT GATCATCAAC AATAATACAG TTTCAGTGGA AGGTCCTGCC
    AATTCTTCAG AAGCTGTTCA GCATTCTGTA AATGAAGGAA TCACAGAGTT ACCTGCTTTG
    GTCTGGCCAG ATATTGAAGG ATCAGATGAA GAATTCAAAG TTTTAGATGA GGACATGGAA
    CTCGTCAAGT TTGTGCCTAC AAAAAGCCCA GTGACTGAAC TCTTAGAAGA AGATTTAGTT
    GGTGATGAAA TATTGGTTGC AACAACAACA ACAACAACAA CTCTATCCTC AACAACAGTA
    GCGACTGTGG TAACAAGTTT AGGTCATTCA GCCTCCCTGA CTCCTGAAAA GGACTCACCT
    TTCACTTGGA TATCACATTC ATCAATAGAT GAGGATGAAC CTGTGCAAGA GTCAACCACA
    GAACCTCTAC CCACCCAATC ACCCACTGCT TCTGTTCTTC CAAAGACAAC TACACAGACA
    TTGATGCACA CATACATAGC AGCTGTTGCA GGGGAGCCTG TTTTATCAGA TAACATGGAT
    AATAAGGACT CAACCACAAT GTCCACCCTT TTAGATGTTT CAAAACATTT AAAGACCCTT
    AGTTCCACCT CTGTACAATC ATTTTCAGAA CCAACATTTA CACCTACTGT CCACATAATT
    AAGCATGGAG AAACTTCAGA TGACTCAGTA CAAGATGTAG TTGAGACTGG AGAAGTTTTA
    GATGACTCTG TACAAGATGT ACTTGAGCAT GAAGACATTT CATATGGCTC TGTACAAGAT
    GTAGTTGTAT CTGGAGAAAC TTCAAATGAC TCAGTACAAG ATGTAGTTGA GACTGGAGAA
    GTTTTAGATG ATTCTGTACA AGATGTACTT GAGCATGAGG ACATTTTATC TGGCCCAGTT
    CAAGATGTAG TTGTGTCTGG AGAAACTGCA GATGACTCAG TACAAGATTT AGTTGAGACT
    GGAGAAGTTT TAGATGATTC CGTACAAGAT GTACTTGAGC ATGAAGTAAT TTCATTTGGC
    CCAGTACAAG ATGTAGTTGT ATCTGGAAAA ACTTCAAATG ACTCAGTACA AGATGTAGTT
    GAGAGTGCAA AAGATTCAGA TGCCCCAATA CAAGATGTAT TTGAGCATAA AGACATTTCA
    GTTGACTCTG TACAAGATGT AGTTGTGTCT GGAGAAACTG CAGATGACTC TATTCAAGAT
    TTATTTAAGC ATGAAGGCAC TTCGTTCGAC ACAGTACAAG ATGAGGTTGA GTCTAGAGAA
    ACTTCAGATG ACTCAGTACA AAATGTAGTT GAGCATGAAG ACTCTTCAGA TGACGCAGTT
    CAAGATGTAG TCGAGTCTGG AGACACTTCA GATGACTCTG TACAAGATAT ATTTGAGTCT
    GCAGAAACTT CAGATGACTC TGTACAAGAT GTAGCTGAGA CTGGAAAAGA ATCAGATGAC
    TTAGTACAAG ATATATTTGA GAATAAAGAC ATTTCAGTTG ACTCAGTACA AGATGTAGTT
    GTATCTGGAG AGACTTCAGA TGACTCTATT CAAGGTTTAC TTGAGCATGA AGGCACTTCA
    GTCGACATAG TACAAGATGA GGTTGAGTTT AGAGAAAATT CAGATGACTC AGTACTAAAT
    GTAGTCAAGC ATGAAGATAC TTCAGATGAC TCAATACAAG ATGTAGTCGA GACAGGAGAA
    GTTTCAGATA GCTCAGTACA AGATGTAGCT GAGTCTGGAG AAACTTTAGA TGACTCTGTT
    CAAGATTTTG TTAAAACTGG AGAAATTTCC ATTGACTCAG TACACAATTT ATTTGAGCCT
    GAAAAAAACG TAGATGACTC TGTACAAGAT GTAGTTGAGA CTGGAGAAGT TTCTGTTGAT
    TCAGTACAAG ATGTAATTGA GCTTGGAGAC ACTTTAGATG ACTCTGCACA AGGAATAGTT
    GAACCTGAAG AAATGGCAGA CAACTTTATA AAGGAAAGCA CTGTAAAGCC AAGACCTGAC
    ATCAAAGGGC TTATTCTACC AACAGAGACA GTGAACTATG AAAGTGCCAC TATGAAAGTT
    CCTGCTCCAG TTAACCCCAG CATAACAGAA TTTAAGGTTT CATTTGACAT CTTCCCTTTC
    GACGACCCAA CTGATGATGA AGATAATGGC AGTGGGTTTG CTCATGGAAC TGACTTAGCA
    AGTTTAGCCT TGCCTGCCAG TCCTGGAAGG GCATTAATAG TGTTTTTTAG CCTTAGGGTC
    ACCAACATGA AGTTTTCCGA AGATCTCTTC AACAAGAGCT CTACTGAATA CAAGGCACTT
    GAGCAGCGAT TTCTGGAACT GCTCGTACCG TATCTACAGT CCAACCTCAG CAATTTCCAG
    AATTTAGAGA TTTTGAACTT CAGGAATGGT AGCATTGTGG TGAACAGCAG GATGAAGTTT
    GCCAAGCCAG TTCCCCATGG TGTGACAACA GCAGTGTACC TAATCTTGGA GGACTTCTGC
    AACACTGCCT ACCAAACTAT GAATCTTGCA ATAGACAAGT ATTCATTGGA TGTCGAATCA
    GGTGACCAGG CAGATCCTTG TAAGTTTCAG GCATGTAACG AGTTCTCCAA GTGTTTGGTG
    AACCGCTGGT CAGGTGAGGC CGAGTGTGTT TGTAACGCTG GTTATTTCAG CGTTGACGGA
    CTGCCATGCC AGAGCATCTG CGACATCCAA GAAGACTTCT GCCTAAATGA CGGGAAATGT
    GACATAATAC CTGGCAAGGG CGCCATCTGC AGGTGTCGCG TTGGTGAAAA CTGGTGGTAT
    CGAGGAGAAC ACTGTGAGGA ATATGTGTCA GAGCCGCTGG TGGTCGGCAT TGCCATTGCG
    TCCGTTGCTG GATTTCTCCT GGTGGCGTCG GGTGTCATCT TTTTCCTGGC CCGAACTCTG
    AGAGACCAGT ATGACACAGA TGATTCAGAA GACCCTTTAC GATATGCAGA GAACTTGCCA
    TCTCTAGAAA GAGCCACCAA GTACAACCCG ATGTTTGAAA GTGAAGTGAC TGCAGGCCAT
    AATCAGTATT ATCGGCGTTA CCCAGAAGCT CCGGCATTCA GCACGGGCAG CACCGAGATC
    TCCACTGACT TCAGCAGCGA AGAGATTCGT CACATCTATC AGAACAGTGA ACTTACTAAA
    GAGGAAATCC AGGACCGTAT TCGTATTATA GAGCTTTACG CCAAGGACCG CCAGTTTGCA
    GATTTTGTCC GACAGCACCA AGTGGCGCTT GACGCTAGAC GAGAGAGCTC TTCCAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146187
    Clone ID Related Accession (Same CDS sequence) XM_021474487.1 , XM_021469613.1 , XM_021473523.1
    Accession Version XM_021473523.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8340bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin homolog isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395115.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGCCTGATT TACCTTTTAA ATGCATTTGG TGGGGTTTGA CACTATTGTT GTTGACTGGA 
    TACTTTTCTA TAGATGCAGA TACTGGAACT CATGAGGATT ACTTGGCAAA TGGAAATACA
    ATGCTTTTGG ACACTAATGA AGTTTTGAAT CACTTATTGT TGTTGGACAA AGGTAAAACT
    TTGAATTACA TGTCTGCTGA AGGGAAAACA GCTCTATCTA GACATAAAAG GGACCTCCTG
    TTCCCCAATG GAGTTAAACT GTGCTCACAT GAGACGGTGC AACAGGCAGT GCAGAATCAT
    CTCAACTACT TTCACCTGCG AGTGTGCCAG GAGACAATTT GGGAGGCTTA TAAAATCTTC
    TGGGATCGCT TACCTGAGAA AGAAGAGTAC CAGATCTGGA TGAAAAAATG TCAAAATGGT
    AGCATTAGCG TGTTTGACAT CGGCCGAAGC TTTAGTCAGT CAGCTGAGCA CCTTGCTTTG
    ATTTCAAGTA GGGTAGCATC ATCCACAATA ACATCAACAA GTGCGCCCAC CAGCCCATGG
    CAACCTGAAT GCAGGCATCA GACGACGACT ACTTCGCCAA CACTAGCAAA AGCTGAACAA
    GTTGGTATTT TGATTCCTGA GGCAGTGCCA GGTTCACAAG TTGTCACACC GCAGACCTTT
    GCAGATGATA TCGTAATCAC AACTTTGCCT ACAAGGCTTC AAGTGACACC TGGAATTACC
    CCAAAGCCTG ATCTTGTCAC AGCCTTTAAG GACACAGTAA AACCTATTTC TGAAACTGAA
    CCTGAGGTCA TATTGGGTAC TACTTCTGAG AGTCCTGTTA AAGCATTGGA TACCATTACC
    ATTAAACATG AAGAGGTTGT TGTAGTCTCA GAATCTAAAT TATTGATTAC TGAGAGGACT
    TCGGAGGACT CTACTTCAAG GAAATATGAT TATGAGTTTC CCACGACCTT TAAGGACTCG
    TTAGTAATAA ACACTCCTAT AGTACTAACA ACTGAATTTT CTGAACAAAT GTATTCAGAA
    ATAGGACAAG GTTTTTCTGA TGGTACAGTT AAAACAGTAG CTGTTACTAT TCAAAAGGAA
    CTTGACATTG TCTCAAAATC TACATTATTA ACCACTGACA AAACTCCAGA AGTTTCTACT
    TCAAAAAAAT CTGAGCATGA TAATCCCACT AACTTTGTAG ATTCATTGGG AATAAGCACC
    AATACTCCTA GAGTGACCAC AAGTGAACTC AAAGAAGATC TTCATTCAGA ATTAGAACAG
    GTGGCTGGGC AGGATGTTAT TCACAGTCCT GTTAAAACAA CACATGTTAC AATTAAGCAT
    GAAGTGTTGG ACACAAATAC TACATTAATG ACAACTGACA GAACTCCATA TTCTACTTCA
    GCAAAATATG AACGTGTTAT ACCAACATCC TTTGAGGACT CACTAGTCAC AAGTTCATCT
    ACTGAACAGG ATATGACCAA TGTTCCTATT AAGACGGCAT ATGCCACAAT TTCACATGGA
    GAGTCTGAAA TTGTCACAGA GTCGACAATA ACAACCACTA ACAGAAATCT TGAAGATTCT
    TCTTTAGCAA AATATGATTA TGGGATTCCT CAAGGAATTG ATACTTCTTC GGGAGTAATT
    AATGAATCTT CTACAAAAGA ACATAATTCA GTATCTAAAT TGAATATTGG TCAGACTACT
    ACTGAAGGTC CCCAAACAAC TGATGCAGTT CAGTATGTAG AATCTGACAT TGTGTCAGAG
    GAAACGTTGA AATCCACTGG CAGAACTGCA GTTAGCTCAA ACTCTACAAA AATTGAGGCC
    GATCTTTCCA CAACTCCTGA GGACCTGACA GGAGTAAGTA CCAACACTCC TGAAATAGTC
    ACTAGAGATT CCATTACAAT ACTTATCCCA GAAACCGAGG GCATTACTAA AAATCCTGTT
    AAATCTGCAC CTATTACCAT TCAGCATGTG GATGTTGATG ATGTTTTAGC AGCTACAGTG
    ATGTCCACTC ATAAACCTCC CAAAGTCTCA CCTACAGAAA TATCCCCCGA TCAGAATCTC
    TTACCAGAGG AATCAAAGCT GCCAACTCCT ACAAAACTAG CAGAAGTTTT GTCTGCCACA
    GAAGAGTTGC CAAAGGATCA GACAGACAAG GATGTTGCTA AAATTGTATA TGTGTCTACT
    GTGCCAGAAC TGGTGTCACC AACTATTCCC GCATATACGT CAACTGACAC TACTAAGGAA
    AACCTACATG TGTTAACAGG GGGGGCAGTT GTTACATCAC TTGATAAAGA TGAGGAAGAA
    CATACACCCC ACACAAAAGA GAGCAAAATT ACACAGGTGA CAACAGGAGC AGAATTACCA
    GGTACCATAA CAGAGAGTAC TCATGGAATG TGGAGGGAAA CCCAAGAGCC AATAAATGTC
    ACACCTGATG CTGAAGACAA TCATTTGGGC AAAGAAGGGT ACATAATAAC AGAGATCCCA
    GAAGTTGCTG AAGTACCAGC TGGAGTGACA CCCAAAATGA TTGTGGAAAC TACAACAGCC
    ACTGAATCTG TGTTTACTGT GAAAGACACA TCTATAATTG TAGACAAAAC TGGCATTGTG
    AAACCTGTCG TGACTACGAC AGAGGCAGCT GAACTTACTG AGGTTGGATT CGAAGATTTA
    GGCACAGACA AAGTAACAAT TCTTGAAAAC ACACTTGAAG ATTCTGTAGG ACTAACAAAT
    GAACCTTTTG CTGTATCATG GGGTGTAACT GAAGCCACTA TTTTAGAGGA ATCATATGTG
    GAAAGTACTG AAAGTATTTC AGGTAAAGAG AAGTCCACTG AAACTATTAA GATCACAGGT
    GAAGCCACAA CTGATGACAT TGGTACTTCT GAAACTGACA TTGTAGTGCC TGTAACCGAT
    CGCATAGAAG ATAATGTAAA TGTATCACCT AAAAATGATG ATGCTAAGGA AACAAACATT
    TTTGAAGACA CAACACCAGA TCTTAGTGTT ACTAATTCTG AAAAAGACAT ACTGACAACT
    ACCATACATA AAGACATGCA TGAAGTTACA GAACCATTAA ATATTCCAGA GGTCACACAT
    AAGGTTGTGA TAGAGATCAC AGATGATGTA CGTGAGACCA GAGGAGATTC CTTACCCGAG
    ACACCAGAAG TCACAACAGA GACACCTAAG GTTGCAACAG AAGCTGATGT CAAAACAACA
    ACAAATGTTA TATCTAAAGC ACCTGAAGTT ATACTGAAGA CAACTGTAAC CAAGTCAGAT
    ACACCTGATG TCACAACTCA GACACCTTTG TTAAAGCTTG AAGATACAGA GAGGGTTGAA
    ATAGTACCTG AAACTCCTGA CATACAAGTG GTTCAAACAG AACACTCAGA AAATGATATC
    CCAACTCCTG TCTTGAAAGT GGAGGATTTC ACAGTTGCGC TGAATGACTT GACAACTCCT
    ATTCAAAAGA CTTCTTCAAC CACATTACAA CAAGACATTT CTAATGATAT TTTAGAAGAA
    AACAATGTGA TGGGAAATGA AATTGATGAT ATTGTGGTTC GACCAGTACG CCCTGTCGGC
    GATCATATTT TGGAGCTAAG TATTAAGCTA AGAGGGGAAA GCTATGACGA TGCTCTGAGG
    GATCCATCAA GTAACTACTA TCAACATCTG TCTACACTTT TTGTTGAAAA GATTGAAGAA
    GTTTTCGAAA GGCTTCCAGG TTTCAAGAAA ATATATGTTC TTGAATTCAG GCCACAGAAG
    GACATTGAAG GAGGTCTGGC AGTGGTGGTG CACTATGCTG TTGTTCTTGA GGGTGATGGG
    TCAGGAGTAA GTAATGAGAC CATGGATTAC ATAAACCTGC ATTCCAACAT GGTTGAGAAC
    TCCTTCACAG ATCCTGAGGA GCTGCCCACT GTGGTCTACA CCATCACTGA CTTCCGAAAC
    TACATCTCCG AAGCCCTTCA CAAGGATAAC TTTATCAGAA ACGCTACTCT GAATGTGGAC
    ATTGATTCTT TGCAACTTGA AAATGTGGAG ACATTGCTGC CTTCCAAACC AACCAGCAGA
    CCGTTTGACT CCAGTGACAT GATGGACAAC GTTCTTGCTT CCGAGAAACC ACCAGATGTT
    CCTGGGCAAG AATTATCCAA CAATGACATC TTCATTACAA ATTATGACAT CTTTGATCCT
    GATCCATGGA CAGGTGGACA AAGTGAAGAC AGAAATGCAA ATGATGTAAT CATCTTTGAG
    GAAAGTCCCA CTCAGTCTCC TGTTGAAGTG TCTTTGAAGA ACTTGGACAT TGAAGTTACT
    TCAAAATCTG AAACTTCAAG CAGTGCCCCT GTTCCTGATG AAGCTGTTAT TGAAGAGGAG
    GGATTTTTAC AGACTGCAAC TACAGTCAGT CCTATTATAG ACACAATAAC TGAATTTACT
    TTACCTACAG AATCACCTCA CAAGGTAGAA GAGACACCAG TTTCTCCTGT AGAACCTCCT
    GAGGTTGAGG AAACTACCCT GGGTGATTTA ACAGACCTTG GCTCAGGCTC AGGATTCTCT
    GGAGATCACA TAGGCCCTGA CATCTGGCCA TGGTCATCAG AAACATCACA AGAAGTCTTG
    AAGGAAGATA CCAATGATGA GATACACCAA CTTCAGGAAA ATCAGGAATC TATAATAAAA
    CAGCTTGAGC CTTTACATCA AGATGTGACT CCTGAGGAGC CATTCTTGGA TAGGGTTCTT
    GTGACCCAAG ACATTCGTAC CAATCCTCAT TACACAACAA CTGACCAGGC CCCTGTATTT
    TGGACAATGG AGACTTTGAC TGTTGAGCTT TCAATGCAGA CTCAAGTAGC CCCTGAACAT
    TATGATGACT ATTTTCCAGA TGCATCAATC ACTATGGCTA CACTTGTAAC CAATCAACCA
    TTGTTACATG TTCACAATCC ATTAGAAACA AAAGATCTGG ATGCATCACA AAGTCCAGGA
    ACAATAAGTT CAACTGCTGA TAAGGACTAT ACAGAACGAC TTGTTACACC CACTGTGATC
    CAACCAAGTG AAACAGTAGC TGTGGATAAG CATACTACAA CAGACAACAA ACAGAATCTG
    GAAACTATGG GTACATTATC TGCAACACTG GGCTCCACAA TGAAGTCCAC TGAAATCTCT
    TCAACTACCC AATTACCTGT GATCATCAAC AATAATACAG TTTCAGTGGA AGGTCCTGCC
    AATTCTTCAG AAGCTGTTCA GCATTCTGTA AATGAAGGAA TCACAGAGTT ACCTGCTTTG
    GTCTGGCCAG ATATTGAAGG ATCAGATGAA GAATTCAAAG TTTTAGATGA GGACATGGAA
    CTCGTCAAGT TTGTGCCTAC AAAAAGCCCA GTGACTGAAC TCTTAGAAGA AGATTTAGTT
    GGTGATGAAA TATTGGTTGC AACAACAACA ACAACAACAA CTCTATCCTC AACAACAGTA
    GCGACTGTGG TAACAAGTTT AGGTCATTCA GCCTCCCTGA CTCCTGAAAA GGACTCACCT
    TTCACTTGGA TATCACATTC ATCAATAGAT GAGGATGAAC CTGTGCAAGA GTCAACCACA
    GAACCTCTAC CCACCCAATC ACCCACTGCT TCTGTTCTTC CAAAGACAAC TACACAGACA
    TTGATGCACA CATACATAGC AGCTGTTGCA GGGGAGCCTG TTTTATCAGA TAACATGGAT
    AATAAGGACT CAACCACAAT GTCCACCCTT TTAGATGTTT CAAAACATTT AAAGACCCTT
    AGTTCCACCT CTGTACAATC ATTTTCAGAA CCAACATTTA CACCTACTGT CCACATAATT
    AAGCATGGAG AAACTTCAGA TGACTCAGTA CAAGATGTAG TTGAGACTGG AGAAGTTTTA
    GATGACTCTG TACAAGATGT ACTTGAGCAT GAAGACATTT CATATGGCTC TGTACAAGAT
    GTAGTTGTAT CTGGAGAAAC TTCAAATGAC TCAGTACAAG ATGTAGTTGA GACTGGAGAA
    GTTTTAGATG ATTCTGTACA AGATGTACTT GAGCATGAGG ACATTTTATC TGGCCCAGTT
    CAAGATGTAG TTGTGTCTGG AGAAACTGCA GATGACTCAG TACAAGATTT AGTTGAGACT
    GGAGAAGTTT TAGATGATTC CGTACAAGAT GTACTTGAGC ATGAAGTAAT TTCATTTGGC
    CCAGTACAAG ATGTAGTTGT ATCTGGAAAA ACTTCAAATG ACTCAGTACA AGATGTAGTT
    GAGAGTGCAA AAGATTCAGA TGCCCCAATA CAAGATGTAT TTGAGCATAA AGACATTTCA
    GTTGACTCTG TACAAGATGT AGTTGTGTCT GGAGAAACTG CAGATGACTC TATTCAAGAT
    TTATTTAAGC ATGAAGGCAC TTCGTTCGAC ACAGTACAAG ATGAGGTTGA GTCTAGAGAA
    ACTTCAGATG ACTCAGTACA AAATGTAGTT GAGCATGAAG ACTCTTCAGA TGACGCAGTT
    CAAGATGTAG TCGAGTCTGG AGACACTTCA GATGACTCTG TACAAGATAT ATTTGAGTCT
    GCAGAAACTT CAGATGACTC TGTACAAGAT GTAGCTGAGA CTGGAAAAGA ATCAGATGAC
    TTAGTACAAG ATATATTTGA GAATAAAGAC ATTTCAGTTG ACTCAGTACA AGATGTAGTT
    GTATCTGGAG AGACTTCAGA TGACTCTATT CAAGGTTTAC TTGAGCATGA AGGCACTTCA
    GTCGACATAG TACAAGATGA GGTTGAGTTT AGAGAAAATT CAGATGACTC AGTACTAAAT
    GTAGTCAAGC ATGAAGATAC TTCAGATGAC TCAATACAAG ATGTAGTCGA GACAGGAGAA
    GTTTCAGATA GCTCAGTACA AGATGTAGCT GAGTCTGGAG AAACTTTAGA TGACTCTGTT
    CAAGATTTTG TTAAAACTGG AGAAATTTCC ATTGACTCAG TACACAATTT ATTTGAGCCT
    GAAAAAAACG TAGATGACTC TGTACAAGAT GTAGTTGAGA CTGGAGAAGT TTCTGTTGAT
    TCAGTACAAG ATGTAATTGA GCTTGGAGAC ACTTTAGATG ACTCTGCACA AGGAATAGTT
    GAACCTGAAG AAATGGCAGA CAACTTTATA AAGGAAAGCA CTGTAAAGCC AAGACCTGAC
    ATCAAAGGGC TTATTCTACC AACAGAGACA GTGAACTATG AAAGTGCCAC TATGAAAGTT
    CCTGCTCCAG TTAACCCCAG CATAACAGAA TTTAAGGTTT CATTTGACAT CTTCCCTTTC
    GACGACCCAA CTGATGATGA AGATAATGGC AGTGGGTTTG CTCATGGAAC TGACTTAGCA
    AGTTTAGCCT TGCCTGCCAG TCCTGGAAGG GCATTAATAG TGTTTTTTAG CCTTAGGGTC
    ACCAACATGA AGTTTTCCGA AGATCTCTTC AACAAGAGCT CTACTGAATA CAAGGCACTT
    GAGCAGCGAT TTCTGGAACT GCTCGTACCG TATCTACAGT CCAACCTCAG CAATTTCCAG
    AATTTAGAGA TTTTGAACTT CAGGAATGGT AGCATTGTGG TGAACAGCAG GATGAAGTTT
    GCCAAGCCAG TTCCCCATGG TGTGACAACA GCAGTGTACC TAATCTTGGA GGACTTCTGC
    AACACTGCCT ACCAAACTAT GAATCTTGCA ATAGACAAGT ATTCATTGGA TGTCGAATCA
    GGTGACCAGG CAGATCCTTG TAAGTTTCAG GCATGTAACG AGTTCTCCAA GTGTTTGGTG
    AACCGCTGGT CAGGTGAGGC CGAGTGTGTT TGTAACGCTG GTTATTTCAG CGTTGACGGA
    CTGCCATGCC AGAGCATCTG CGACATCCAA GAAGACTTCT GCCTAAATGA CGGGAAATGT
    GACATAATAC CTGGCAAGGG CGCCATCTGC AGGTGTCGCG TTGGTGAAAA CTGGTGGTAT
    CGAGGAGAAC ACTGTGAGGA ATATGTGTCA GAGCCGCTGG TGGTCGGCAT TGCCATTGCG
    TCCGTTGCTG GATTTCTCCT GGTGGCGTCG GGTGTCATCT TTTTCCTGGC CCGAACTCTG
    AGAGACCAGT ATGACACAGA TGATTCAGAA GACCCTTTAC GATATGCAGA GAACTTGCCA
    TCTCTAGAAA GAGCCACCAA GTACAACCCG ATGTTTGAAA GTGAAGTGAC TGCAGGCCAT
    AATCAGTATT ATCGGCGTTA CCCAGAAGCT CCGGCATTCA GCACGGGCAG CACCGAGATC
    TCCACTGACT TCAGCAGCGA AGAGATTCGT CACATCTATC AGAACAGTGA ACTTACTAAA
    GAGGAAATCC AGGACCGTAT TCGTATTATA GAGCTTTACG CCAAGGACCG CCAGTTTGCA
    GATTTTGTCC GACAGCACCA AGTGGCGCTT GACGCTAGAC GAGAGAGCTC TTCCAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329198.1
    CDS234..8573
    Translation

    Target ORF information:

    RefSeq Version XM_021473523.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio interphotoreceptor matrix proteoglycan 2b (impg2b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473523.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGCCTGATT TACCTTTTAA ATGCATTTGG TGGGGTTTGA CACTATTGTT GTTGACTGGA 
    TACTTTTCTA TAGATGCAGA TACTGGAACT CATGAGGATT ACTTGGCAAA TGGAAATACA
    ATGCTTTTGG ACACTAATGA AGTTTTGAAT CACTTATTGT TGTTGGACAA AGGTAAAACT
    TTGAATTACA TGTCTGCTGA AGGGAAAACA GCTCTATCTA GACATAAAAG GGACCTCCTG
    TTCCCCAATG GAGTTAAACT GTGCTCACAT GAGACGGTGC AACAGGCAGT GCAGAATCAT
    CTCAACTACT TTCACCTGCG AGTGTGCCAG GAGACAATTT GGGAGGCTTA TAAAATCTTC
    TGGGATCGCT TACCTGAGAA AGAAGAGTAC CAGATCTGGA TGAAAAAATG TCAAAATGGT
    AGCATTAGCG TGTTTGACAT CGGCCGAAGC TTTAGTCAGT CAGCTGAGCA CCTTGCTTTG
    ATTTCAAGTA GGGTAGCATC ATCCACAATA ACATCAACAA GTGCGCCCAC CAGCCCATGG
    CAACCTGAAT GCAGGCATCA GACGACGACT ACTTCGCCAA CACTAGCAAA AGCTGAACAA
    GTTGGTATTT TGATTCCTGA GGCAGTGCCA GGTTCACAAG TTGTCACACC GCAGACCTTT
    GCAGATGATA TCGTAATCAC AACTTTGCCT ACAAGGCTTC AAGTGACACC TGGAATTACC
    CCAAAGCCTG ATCTTGTCAC AGCCTTTAAG GACACAGTAA AACCTATTTC TGAAACTGAA
    CCTGAGGTCA TATTGGGTAC TACTTCTGAG AGTCCTGTTA AAGCATTGGA TACCATTACC
    ATTAAACATG AAGAGGTTGT TGTAGTCTCA GAATCTAAAT TATTGATTAC TGAGAGGACT
    TCGGAGGACT CTACTTCAAG GAAATATGAT TATGAGTTTC CCACGACCTT TAAGGACTCG
    TTAGTAATAA ACACTCCTAT AGTACTAACA ACTGAATTTT CTGAACAAAT GTATTCAGAA
    ATAGGACAAG GTTTTTCTGA TGGTACAGTT AAAACAGTAG CTGTTACTAT TCAAAAGGAA
    CTTGACATTG TCTCAAAATC TACATTATTA ACCACTGACA AAACTCCAGA AGTTTCTACT
    TCAAAAAAAT CTGAGCATGA TAATCCCACT AACTTTGTAG ATTCATTGGG AATAAGCACC
    AATACTCCTA GAGTGACCAC AAGTGAACTC AAAGAAGATC TTCATTCAGA ATTAGAACAG
    GTGGCTGGGC AGGATGTTAT TCACAGTCCT GTTAAAACAA CACATGTTAC AATTAAGCAT
    GAAGTGTTGG ACACAAATAC TACATTAATG ACAACTGACA GAACTCCATA TTCTACTTCA
    GCAAAATATG AACGTGTTAT ACCAACATCC TTTGAGGACT CACTAGTCAC AAGTTCATCT
    ACTGAACAGG ATATGACCAA TGTTCCTATT AAGACGGCAT ATGCCACAAT TTCACATGGA
    GAGTCTGAAA TTGTCACAGA GTCGACAATA ACAACCACTA ACAGAAATCT TGAAGATTCT
    TCTTTAGCAA AATATGATTA TGGGATTCCT CAAGGAATTG ATACTTCTTC GGGAGTAATT
    AATGAATCTT CTACAAAAGA ACATAATTCA GTATCTAAAT TGAATATTGG TCAGACTACT
    ACTGAAGGTC CCCAAACAAC TGATGCAGTT CAGTATGTAG AATCTGACAT TGTGTCAGAG
    GAAACGTTGA AATCCACTGG CAGAACTGCA GTTAGCTCAA ACTCTACAAA AATTGAGGCC
    GATCTTTCCA CAACTCCTGA GGACCTGACA GGAGTAAGTA CCAACACTCC TGAAATAGTC
    ACTAGAGATT CCATTACAAT ACTTATCCCA GAAACCGAGG GCATTACTAA AAATCCTGTT
    AAATCTGCAC CTATTACCAT TCAGCATGTG GATGTTGATG ATGTTTTAGC AGCTACAGTG
    ATGTCCACTC ATAAACCTCC CAAAGTCTCA CCTACAGAAA TATCCCCCGA TCAGAATCTC
    TTACCAGAGG AATCAAAGCT GCCAACTCCT ACAAAACTAG CAGAAGTTTT GTCTGCCACA
    GAAGAGTTGC CAAAGGATCA GACAGACAAG GATGTTGCTA AAATTGTATA TGTGTCTACT
    GTGCCAGAAC TGGTGTCACC AACTATTCCC GCATATACGT CAACTGACAC TACTAAGGAA
    AACCTACATG TGTTAACAGG GGGGGCAGTT GTTACATCAC TTGATAAAGA TGAGGAAGAA
    CATACACCCC ACACAAAAGA GAGCAAAATT ACACAGGTGA CAACAGGAGC AGAATTACCA
    GGTACCATAA CAGAGAGTAC TCATGGAATG TGGAGGGAAA CCCAAGAGCC AATAAATGTC
    ACACCTGATG CTGAAGACAA TCATTTGGGC AAAGAAGGGT ACATAATAAC AGAGATCCCA
    GAAGTTGCTG AAGTACCAGC TGGAGTGACA CCCAAAATGA TTGTGGAAAC TACAACAGCC
    ACTGAATCTG TGTTTACTGT GAAAGACACA TCTATAATTG TAGACAAAAC TGGCATTGTG
    AAACCTGTCG TGACTACGAC AGAGGCAGCT GAACTTACTG AGGTTGGATT CGAAGATTTA
    GGCACAGACA AAGTAACAAT TCTTGAAAAC ACACTTGAAG ATTCTGTAGG ACTAACAAAT
    GAACCTTTTG CTGTATCATG GGGTGTAACT GAAGCCACTA TTTTAGAGGA ATCATATGTG
    GAAAGTACTG AAAGTATTTC AGGTAAAGAG AAGTCCACTG AAACTATTAA GATCACAGGT
    GAAGCCACAA CTGATGACAT TGGTACTTCT GAAACTGACA TTGTAGTGCC TGTAACCGAT
    CGCATAGAAG ATAATGTAAA TGTATCACCT AAAAATGATG ATGCTAAGGA AACAAACATT
    TTTGAAGACA CAACACCAGA TCTTAGTGTT ACTAATTCTG AAAAAGACAT ACTGACAACT
    ACCATACATA AAGACATGCA TGAAGTTACA GAACCATTAA ATATTCCAGA GGTCACACAT
    AAGGTTGTGA TAGAGATCAC AGATGATGTA CGTGAGACCA GAGGAGATTC CTTACCCGAG
    ACACCAGAAG TCACAACAGA GACACCTAAG GTTGCAACAG AAGCTGATGT CAAAACAACA
    ACAAATGTTA TATCTAAAGC ACCTGAAGTT ATACTGAAGA CAACTGTAAC CAAGTCAGAT
    ACACCTGATG TCACAACTCA GACACCTTTG TTAAAGCTTG AAGATACAGA GAGGGTTGAA
    ATAGTACCTG AAACTCCTGA CATACAAGTG GTTCAAACAG AACACTCAGA AAATGATATC
    CCAACTCCTG TCTTGAAAGT GGAGGATTTC ACAGTTGCGC TGAATGACTT GACAACTCCT
    ATTCAAAAGA CTTCTTCAAC CACATTACAA CAAGACATTT CTAATGATAT TTTAGAAGAA
    AACAATGTGA TGGGAAATGA AATTGATGAT ATTGTGGTTC GACCAGTACG CCCTGTCGGC
    GATCATATTT TGGAGCTAAG TATTAAGCTA AGAGGGGAAA GCTATGACGA TGCTCTGAGG
    GATCCATCAA GTAACTACTA TCAACATCTG TCTACACTTT TTGTTGAAAA GATTGAAGAA
    GTTTTCGAAA GGCTTCCAGG TTTCAAGAAA ATATATGTTC TTGAATTCAG GCCACAGAAG
    GACATTGAAG GAGGTCTGGC AGTGGTGGTG CACTATGCTG TTGTTCTTGA GGGTGATGGG
    TCAGGAGTAA GTAATGAGAC CATGGATTAC ATAAACCTGC ATTCCAACAT GGTTGAGAAC
    TCCTTCACAG ATCCTGAGGA GCTGCCCACT GTGGTCTACA CCATCACTGA CTTCCGAAAC
    TACATCTCCG AAGCCCTTCA CAAGGATAAC TTTATCAGAA ACGCTACTCT GAATGTGGAC
    ATTGATTCTT TGCAACTTGA AAATGTGGAG ACATTGCTGC CTTCCAAACC AACCAGCAGA
    CCGTTTGACT CCAGTGACAT GATGGACAAC GTTCTTGCTT CCGAGAAACC ACCAGATGTT
    CCTGGGCAAG AATTATCCAA CAATGACATC TTCATTACAA ATTATGACAT CTTTGATCCT
    GATCCATGGA CAGGTGGACA AAGTGAAGAC AGAAATGCAA ATGATGTAAT CATCTTTGAG
    GAAAGTCCCA CTCAGTCTCC TGTTGAAGTG TCTTTGAAGA ACTTGGACAT TGAAGTTACT
    TCAAAATCTG AAACTTCAAG CAGTGCCCCT GTTCCTGATG AAGCTGTTAT TGAAGAGGAG
    GGATTTTTAC AGACTGCAAC TACAGTCAGT CCTATTATAG ACACAATAAC TGAATTTACT
    TTACCTACAG AATCACCTCA CAAGGTAGAA GAGACACCAG TTTCTCCTGT AGAACCTCCT
    GAGGTTGAGG AAACTACCCT GGGTGATTTA ACAGACCTTG GCTCAGGCTC AGGATTCTCT
    GGAGATCACA TAGGCCCTGA CATCTGGCCA TGGTCATCAG AAACATCACA AGAAGTCTTG
    AAGGAAGATA CCAATGATGA GATACACCAA CTTCAGGAAA ATCAGGAATC TATAATAAAA
    CAGCTTGAGC CTTTACATCA AGATGTGACT CCTGAGGAGC CATTCTTGGA TAGGGTTCTT
    GTGACCCAAG ACATTCGTAC CAATCCTCAT TACACAACAA CTGACCAGGC CCCTGTATTT
    TGGACAATGG AGACTTTGAC TGTTGAGCTT TCAATGCAGA CTCAAGTAGC CCCTGAACAT
    TATGATGACT ATTTTCCAGA TGCATCAATC ACTATGGCTA CACTTGTAAC CAATCAACCA
    TTGTTACATG TTCACAATCC ATTAGAAACA AAAGATCTGG ATGCATCACA AAGTCCAGGA
    ACAATAAGTT CAACTGCTGA TAAGGACTAT ACAGAACGAC TTGTTACACC CACTGTGATC
    CAACCAAGTG AAACAGTAGC TGTGGATAAG CATACTACAA CAGACAACAA ACAGAATCTG
    GAAACTATGG GTACATTATC TGCAACACTG GGCTCCACAA TGAAGTCCAC TGAAATCTCT
    TCAACTACCC AATTACCTGT GATCATCAAC AATAATACAG TTTCAGTGGA AGGTCCTGCC
    AATTCTTCAG AAGCTGTTCA GCATTCTGTA AATGAAGGAA TCACAGAGTT ACCTGCTTTG
    GTCTGGCCAG ATATTGAAGG ATCAGATGAA GAATTCAAAG TTTTAGATGA GGACATGGAA
    CTCGTCAAGT TTGTGCCTAC AAAAAGCCCA GTGACTGAAC TCTTAGAAGA AGATTTAGTT
    GGTGATGAAA TATTGGTTGC AACAACAACA ACAACAACAA CTCTATCCTC AACAACAGTA
    GCGACTGTGG TAACAAGTTT AGGTCATTCA GCCTCCCTGA CTCCTGAAAA GGACTCACCT
    TTCACTTGGA TATCACATTC ATCAATAGAT GAGGATGAAC CTGTGCAAGA GTCAACCACA
    GAACCTCTAC CCACCCAATC ACCCACTGCT TCTGTTCTTC CAAAGACAAC TACACAGACA
    TTGATGCACA CATACATAGC AGCTGTTGCA GGGGAGCCTG TTTTATCAGA TAACATGGAT
    AATAAGGACT CAACCACAAT GTCCACCCTT TTAGATGTTT CAAAACATTT AAAGACCCTT
    AGTTCCACCT CTGTACAATC ATTTTCAGAA CCAACATTTA CACCTACTGT CCACATAATT
    AAGCATGGAG AAACTTCAGA TGACTCAGTA CAAGATGTAG TTGAGACTGG AGAAGTTTTA
    GATGACTCTG TACAAGATGT ACTTGAGCAT GAAGACATTT CATATGGCTC TGTACAAGAT
    GTAGTTGTAT CTGGAGAAAC TTCAAATGAC TCAGTACAAG ATGTAGTTGA GACTGGAGAA
    GTTTTAGATG ATTCTGTACA AGATGTACTT GAGCATGAGG ACATTTTATC TGGCCCAGTT
    CAAGATGTAG TTGTGTCTGG AGAAACTGCA GATGACTCAG TACAAGATTT AGTTGAGACT
    GGAGAAGTTT TAGATGATTC CGTACAAGAT GTACTTGAGC ATGAAGTAAT TTCATTTGGC
    CCAGTACAAG ATGTAGTTGT ATCTGGAAAA ACTTCAAATG ACTCAGTACA AGATGTAGTT
    GAGAGTGCAA AAGATTCAGA TGCCCCAATA CAAGATGTAT TTGAGCATAA AGACATTTCA
    GTTGACTCTG TACAAGATGT AGTTGTGTCT GGAGAAACTG CAGATGACTC TATTCAAGAT
    TTATTTAAGC ATGAAGGCAC TTCGTTCGAC ACAGTACAAG ATGAGGTTGA GTCTAGAGAA
    ACTTCAGATG ACTCAGTACA AAATGTAGTT GAGCATGAAG ACTCTTCAGA TGACGCAGTT
    CAAGATGTAG TCGAGTCTGG AGACACTTCA GATGACTCTG TACAAGATAT ATTTGAGTCT
    GCAGAAACTT CAGATGACTC TGTACAAGAT GTAGCTGAGA CTGGAAAAGA ATCAGATGAC
    TTAGTACAAG ATATATTTGA GAATAAAGAC ATTTCAGTTG ACTCAGTACA AGATGTAGTT
    GTATCTGGAG AGACTTCAGA TGACTCTATT CAAGGTTTAC TTGAGCATGA AGGCACTTCA
    GTCGACATAG TACAAGATGA GGTTGAGTTT AGAGAAAATT CAGATGACTC AGTACTAAAT
    GTAGTCAAGC ATGAAGATAC TTCAGATGAC TCAATACAAG ATGTAGTCGA GACAGGAGAA
    GTTTCAGATA GCTCAGTACA AGATGTAGCT GAGTCTGGAG AAACTTTAGA TGACTCTGTT
    CAAGATTTTG TTAAAACTGG AGAAATTTCC ATTGACTCAG TACACAATTT ATTTGAGCCT
    GAAAAAAACG TAGATGACTC TGTACAAGAT GTAGTTGAGA CTGGAGAAGT TTCTGTTGAT
    TCAGTACAAG ATGTAATTGA GCTTGGAGAC ACTTTAGATG ACTCTGCACA AGGAATAGTT
    GAACCTGAAG AAATGGCAGA CAACTTTATA AAGGAAAGCA CTGTAAAGCC AAGACCTGAC
    ATCAAAGGGC TTATTCTACC AACAGAGACA GTGAACTATG AAAGTGCCAC TATGAAAGTT
    CCTGCTCCAG TTAACCCCAG CATAACAGAA TTTAAGGTTT CATTTGACAT CTTCCCTTTC
    GACGACCCAA CTGATGATGA AGATAATGGC AGTGGGTTTG CTCATGGAAC TGACTTAGCA
    AGTTTAGCCT TGCCTGCCAG TCCTGGAAGG GCATTAATAG TGTTTTTTAG CCTTAGGGTC
    ACCAACATGA AGTTTTCCGA AGATCTCTTC AACAAGAGCT CTACTGAATA CAAGGCACTT
    GAGCAGCGAT TTCTGGAACT GCTCGTACCG TATCTACAGT CCAACCTCAG CAATTTCCAG
    AATTTAGAGA TTTTGAACTT CAGGAATGGT AGCATTGTGG TGAACAGCAG GATGAAGTTT
    GCCAAGCCAG TTCCCCATGG TGTGACAACA GCAGTGTACC TAATCTTGGA GGACTTCTGC
    AACACTGCCT ACCAAACTAT GAATCTTGCA ATAGACAAGT ATTCATTGGA TGTCGAATCA
    GGTGACCAGG CAGATCCTTG TAAGTTTCAG GCATGTAACG AGTTCTCCAA GTGTTTGGTG
    AACCGCTGGT CAGGTGAGGC CGAGTGTGTT TGTAACGCTG GTTATTTCAG CGTTGACGGA
    CTGCCATGCC AGAGCATCTG CGACATCCAA GAAGACTTCT GCCTAAATGA CGGGAAATGT
    GACATAATAC CTGGCAAGGG CGCCATCTGC AGGTGTCGCG TTGGTGAAAA CTGGTGGTAT
    CGAGGAGAAC ACTGTGAGGA ATATGTGTCA GAGCCGCTGG TGGTCGGCAT TGCCATTGCG
    TCCGTTGCTG GATTTCTCCT GGTGGCGTCG GGTGTCATCT TTTTCCTGGC CCGAACTCTG
    AGAGACCAGT ATGACACAGA TGATTCAGAA GACCCTTTAC GATATGCAGA GAACTTGCCA
    TCTCTAGAAA GAGCCACCAA GTACAACCCG ATGTTTGAAA GTGAAGTGAC TGCAGGCCAT
    AATCAGTATT ATCGGCGTTA CCCAGAAGCT CCGGCATTCA GCACGGGCAG CACCGAGATC
    TCCACTGACT TCAGCAGCGA AGAGATTCGT CACATCTATC AGAACAGTGA ACTTACTAAA
    GAGGAAATCC AGGACCGTAT TCGTATTATA GAGCTTTACG CCAAGGACCG CCAGTTTGCA
    GATTTTGTCC GACAGCACCA AGTGGCGCTT GACGCTAGAC GAGAGAGCTC TTCCAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27099
    Clone ID Related Accession (Same CDS sequence) XM_021474484.1 , XM_009296447.3 , XM_009296447.2 , XM_021473520.1
    Accession Version XM_021474484.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8466bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGCCTGATT TACCTTTTAA ATGCATTTGG TGGGGTTTGA CACTATTGTT GTTGACTGGA 
    TACTTTTCTA TAGATGCAGA TACTGGAACT CATGAGGATT ACTTGGCAAA TGGAAATACA
    ATGCTTTTGG ACACTAATGA AGTTTTGAAT CACTTATTGT TGTTGGACAA AGGTAAAACT
    TTGAATTACA TGTCTGCTGA AGGGAAAACA GCTCTATCTA GACATAAAAG GGACCTCCTG
    TTCCCCAATG GAGTTAAACT GTGCTCACAT GAGACGGTGC AACAGGCAGT GCAGAATCAT
    CTCAACTACT TTCACCTGCG AGTGTGCCAG GAGACAATTT GGGAGGCTTA TAAAATCTTC
    TGGGATCGCT TACCTGAGAA AGAAGAGTAC CAGATCTGGA TGAAAAAATG TCAAAATGGT
    AGCATTAGCG TGTTTGACAT CGGCCGAAGC TTTAGTCAGT CAGCTGAGCA CCTTGCTTTG
    ATTTCAAGTA GGGTAGCATC ATCCACAATA ACATCAACAA GTGCGCCCAC CAGCCCATGG
    CAACCTGAAT GCAGGCATCA GACGACGACT ACTTCGCCAA CACTAGCAAA AGCTGAACAA
    GTTGGTATTT TGATTCCTGA GGCAGTGCCA GGTTCACAAG TTGTCACACC GCAGACCTTT
    GCAGATGATA TCGTAATCAC AACTTTGCCT ACAAGGCTTC AAGTGACACC TGGAATTACC
    CCAAAGCCTG ATCTTGTCAC AGCCTTTAAG GACACAGTAA AACCTATTTC TGAAACTGAA
    CCTGAGGTCA TATTGGGTAC TACTTCTGAG AGTCCTGTTA AAGCATTGGA TACCATTACC
    ATTAAACATG AAGAGGTTGT TGTAGTCTCA GAATCTAAAT TATTGATTAC TGAGAGGACT
    TCGGAGGACT CTACTTCAAG GAAATATGAT TATGAGTTTC CCACGACCTT TAAGGACTCG
    TTAGTAATAA ACACTCCTAT AGTACTAACA ACTGAATTTT CTGAACAAAT GTATTCAGAA
    ATAGGACAAG GTTTTTCTGA TGGTACAGTT AAAACAGTAG CTGTTACTAT TCAAAAGGAA
    CTTGACATTG TCTCAAAATC TACATTATTA ACCACTGACA AAACTCCAGA AGTTTCTACT
    TCAAAAAAAT CTGAGCATGA TAATCCCACT AACTTTGTAG ATTCATTGGG AATAAGCACC
    AATACTCCTA GAGTGACCAC AAGTGAACTC AAAGAAGATC TTCATTCAGA ATTAGAACAG
    GTGGCTGGGC AGGATGTTAT TCACAGTCCT GTTAAAACAA CACATGTTAC AATTAAGCAT
    GAAGTGTTGG ACACAAATAC TACATTAATG ACAACTGACA GAACTCCATA TTCTACTTCA
    GCAAAATATG AACGTGTTAT ACCAACATCC TTTGAGGACT CACTAGTCAC AAGTTCATCT
    ACTGAACAGG ATATGACCAA TGTTCCTATT AAGACGGCAT ATGCCACAAT TTCACATGGA
    GAGTCTGAAA TTGTCACAGA GTCGACAATA ACAACCACTA ACAGAAATCT TGAAGATTCT
    TCTTTAGCAA AATATGATTA TGGGATTCCT CAAGGAATTG ATACTTCTTC GGGAGTAATT
    AATGAATCTT CTACAAAAGA ACATAATTCA GTATCTAAAT TGAATATTGG TCAGACTACT
    ACTGAAGGTC CCCAAACAAC TGATGCAGTT CAGTATGTAG AATCTGACAT TGTGTCAGAG
    GAAACGTTGA AATCCACTGG CAGAACTGCA GTTAGCTCAA ACTCTACAAA AATTGAGGCC
    GATCTTTCCA CAACTCCTGA GGACCTGACA GGAGTAAGTA CCAACACTCC TGAAATAGTC
    ACTAGAGATT CCATTACAAT ACTTATCCCA GAAACCGAGG GCATTACTAA AAATCCTGTT
    AAATCTGCAC CTATTACCAT TCAGCATGTG GATGTTGATG ATGTTTTAGC AGCTACAGTG
    ATGTCCACTC ATAAACCTCC CAAAGTCTCA CCTACAGAAA TATCCCCCGA TCAGAATCTC
    TTACCAGAGG AATCAAAGCT GCCAACTCCT ACAAAACTAG CAGAAGTTTT GTCTGCCACA
    GAAGAGTTGC CAAAGGATCA GACAGACAAG GATGTTGCTA AAATTGTATA TGTGTCTACT
    GTGCCAGAAC TGGTGTCACC AACTATTCCC GCATATACGT CAACTGACAC TACTAAGGAA
    AACCTACATG TGTTAACAGG GGGGGCAGTT GTTACATCAC TTGATAAAGA TGAGGAAGAA
    CATACACCCC ACACAAAAGA GAGCAAAATT ACACAGGTGA CAACAGGAGC AGAATTACCA
    GGTACCATAA CAGAGAGTAC TCATGGAATG TGGAGGGAAA CCCAAGAGCC AATAAATGTC
    ACACCTGATG CTGAAGACAA TCATTTGGGC AAAGAAGGGT ACATAATAAC AGAGATCCCA
    GAAGTTGCTG AAGTACCAGC TGGAGTGACA CCCAAAATGA TTGTGGAAAC TACAACAGCC
    ACTGAATCTG TGTTTACTGT GAAAGACACA TCTATAATTG TAGACAAAAC TGGCATTGTG
    AAACCTGTCG TGACTACGAC AGAGGCAGCT GAACTTACTG AGGTTGGATT CGAAGATTTA
    GGCACAGACA AAGTAACAAT TCTTGAAAAC ACACTTGAAG ATTCTGTAGG ACTAACAAAT
    GAACCTTTTG CTGTATCATG GGGTGTAACT GAAGCCACTA TTTTAGAGGA ATCATATGTG
    GAAAGTACTG AAAGTATTTC AGGTAAAGAG AAGTCCACTG AAACTATTAA GATCACAGGT
    GAAGCCACAA CTGATGACAT TGGTACTTCT GAAACTGACA TTGTAGTGCC TGTAACCGAT
    CGCATAGAAG ATAATGTAAA TGTATCACCT AAAAATGATG ATGCTAAGGA AACAAACATT
    TTTGAAGACA CAACACCAGA TCTTAGTGTT ACTAATTCTG AAAAAGACAT ACTGACAACT
    ACCATACATA AAGACATGCA TGAAGTTACA GAACCATTAA ATATTCCAGA GGTCACACAT
    AAGGTTGTGA TAGAGATCAC AGATGATGTA CGTGAGACCA GAGGAGATTC CTTACCCGAG
    ACACCAGAAG TCACAACAGA GACACCTAAG GTTGCAACAG AAGCTGATGT CAAAACAACA
    ACAAATGTTA TATCTAAAGC ACCTGAAGTT ATACTGAAGA CAACTGTAAC CAAGTCAGAT
    ACACCTGATG TCACAACTCA GACACCTTTG TTAAAGCTTG AAGATACAGA GAGGGTTGAA
    ATAGTACCTG AAACTCCTGA CATACAAGTG GTTCAAACAG AACACTCAGA AAATGATATC
    CCAACTCCTG TCTTGAAAGT GGAGGATTTC ACAGTTGCGC TGAATGACTT GACAACTCCT
    ATTCAAAAGA CTTCTTCAAC CACATTACAA CAAGACATTT CTAATGATAT TTTAGAAGAA
    AACAATGTGA TGGGAAATGA AATTGATGAT ATTGTGGTTC GACCAGTACG CCCTGTCGGC
    GATCATATTT TGGAGCTAAG TATTAAGCTA AGAGGGGAAA GCTATGACGA TGCTCTGAGG
    GATCCATCAA GTAACTACTA TCAACATCTG TCTACACTTT TTGTTGAAAA GATTGAAGAA
    GTTTTCGAAA GGCTTCCAGG TTTCAAGAAA ATATATGTTC TTGAATTCAG GCCACAGAAG
    GACATTGAAG GAGGTCTGGC AGTGGTGGTG CACTATGCTG TTGTTCTTGA GGGTGATGGG
    TCAGGAGTAA GTAATGAGAC CATGGATTAC ATAAACCTGC ATTCCAACAT GGTTGAGAAC
    TCCTTCACAG ATCCTGAGGA GCTGCCCACT GTGGTCTACA CCATCACTGA CTTCCGAAAC
    TACATCTCCG AAGCCCTTCA CAAGGATAAC TTTATCAGAA ACGCTACTCT GAATGTGGAC
    ATTGATTCTT TGCAACTTGA AAATGTGGAG ACATTGCTGC CTTCCAAACC AACCAGCAGA
    CCGTTTGACT CCAGTGACAT GATGGACAAC GTTCTTGCTT CCGAGAAACC ACCAGATGTT
    CCTGGGCAAG AATTATCCAA CAATGACATC TTCATTACAA ATTATGACAT CTTTGATCCT
    GATCCATGGA CAGGTGGACA AAGTGAAGAC AGAAATGCAA ATGATGTAAT CATCTTTGAG
    GAAAGTCCCA CTCAGTCTCC TGTTGAAGTG TCTTTGAAGA ACTTGGACAT TGAAGTTACT
    TCAAAATCTG AAACTTCAAG CAGTGCCCCT GTTCCTGATG AAGCTGTTAT TGAAGAGGAG
    GGATTTTTAC AGACTGCAAC TACAGTCAGT CCTATTATAG ACACAATAAC TGAATTTACT
    TTACCTACAG AATCACCTCA CAAGGTAGAA GAGACACCAG TTTCTCCTGT AGAACCTCCT
    GAGGTTGAGG AAACTACCCT GGGTGATTTA ACAGACCTTG GCTCAGGCTC AGGATTCTCT
    GGAGATCACA TAGGCCCTGA CATCTGGCCA TGGTCATCAG AAACATCACA AGAAGTCTTG
    AAGGAAGATA CCAATGATGA GATACACCAA CTTCAGGAAA ATCAGGAATC TATAATAAAA
    CAGCTTGAGC CTTTACATCA AGATGTGACT CCTGAGGAGC CATTCTTGGA TAGGGTTCTT
    GTGACCCAAG ACATTCGTAC CAATCCTCAT TACACAACAA CTGACCAGGC CCCTGTATTT
    TGGACAATGG AGACTTTGAC TGTTGAGCTT TCAATGCAGA CTCAAGTAGC CCCTGAACAT
    TATGATGACT ATTTTCCAGA TGCATCAATC ACTATGGCTA CACTTGTAAC CAATCAACCA
    TTGTTACATG TTCACAATCC ATTAGAAACA AAAGATCTGG ATGCATCACA AAGTCCAGGA
    ACAATAAGTT CAACTGCTGA TAAGGACTAT ACAGAACGAC TTGTTACACC CACTGTGATC
    CAACCAAGTG AAACAGTAGC TGTGGATAAG CATACTACAA CAGACAACAA ACAGAATCTG
    GAAACTATGG GTACATTATC TGCAACACTG GGCTCCACAA TGAAGTCCAC TGAAATCTCT
    TCAACTACCC AATTACCTGT GATCATCAAC AATAATACAG TTTCAGTGGA AGGTCCTGCC
    AATTCTTCAG AAGCTGTTCA GCATTCTGTA AATGAAGGAA TCACAGAGTT ACCTGCTTTG
    GTCTGGCCAG ATATTGAAGG ATCAGATGAA GAATTCAAAG TTTTAGATGA GGACATGGAA
    CTCGTCAAGT TTGTGCCTAC AAAAAGCCCA GTGACTGAAC TCTTAGAAGA AGATTTAGTT
    GGTGATGAAA TATTGGTTGC AACAACAACA ACAACAACAA CTCTATCCTC AACAACAGTA
    GCGACTGTGG TAACAAGTTT AGGTCATTCA GCCTCCCTGA CTCCTGAAAA GGACTCACCT
    TTCACTTGGA TATCACATTC ATCAATAGAT GAGGATGAAC CTGTGCAAGA GTCAACCACA
    GAACCTCTAC CCACCCAATC ACCCACTGCT TCTGTTCTTC CAAAGACAAC TACACAGACA
    TTGATGCACA CATACATAGC AGCTGTTGCA GGGGAGCCTG TTTTATCAGA TAACATGGAT
    AATAAGGACT CAACCACAAT GTCCACCCTT TTAGATGTTT CAAAACATTT AAAGACCCTT
    AGTTCCACCT CTGTACAATC ATTTTCAGAA CCAACATTTA CACCTACTGT CCACATAATT
    AAGCATGGAG AAACTTCAGA TGACTCAGTA CAAGATGTAG TTGAGACTGG AGAAGTTTTA
    GATGACTCTG TACAAGATGT ACTTGAGCAT GAAGACATTT CATATGGCTC TGTACAAGAT
    GTAGTTGTAT CTGGAGAAAC TTCAAATGAC TCAGTACAAG ATGTAGTTGA GACTGGAGAA
    GTTTTAGATG ATTCTGTACA AGATGTACTT GAGCATGAGG ACATTTTATC TGGCCCAGTT
    CAAGATGTAG TTGTGTCTGG AGAAACTGCA GAAGACTCAG TACAAGATTT AGTTGAGACT
    AGAGAAGTTT TAGATGATTC TGTACAAGAT GTACTTGAGC ATGAGGACAT TTTATCTGGC
    CCAGTTCAAG ATGTAGTTGT GTCTGGAGAA ACTGCAGATG ACTCAGTACA AGATTTAGTT
    GAGACTGGAG AAGTTTTAGA TGATTCCGTA CAAGATGTAC TTGAGCATGA AGTAATTTCA
    TTTGGCCCAG TACAAGATGT AGTTGTATCT GGAAAAACTT CAAATGACTC AGTACAAGAT
    GTAGTTGAGA GTGCAAAAGA TTCAGATGCC CCAATACAAG ATGTATTTGA GCATAAAGAC
    ATTTCAGTTG ACTCTGTACA AGATGTAGTT GTGTCTGGAG AAACTGCAGA TGACTCTATT
    CAAGATTTAT TTAAGCATGA AGGCACTTCG TTCGACACAG TACAAGATGA GGTTGAGTCT
    AGAGAAACTT CAGATGACTC AGTACAAAAT GTAGTTGAGC ATGAAGACTC TTCAGATGAC
    GCAGTTCAAG ATGTAGTCGA GTCTGGAGAC ACTTCAGATG ACTCTGTACA AGATATATTT
    GAGTCTGCAG AAACTTCAGA TGACTCTGTA CAAGATGTAG CTGAGACTGG AAAAGAATCA
    GATGACTTAG TACAAGATAT ATTTGAGAAT AAAGACATTT CAGTTGACTC AGTACAAGAT
    GTAGTTGTAT CTGGAGAGAC TTCAGATGAC TCTATTCAAG GTTTACTTGA GCATGAAGGC
    ACTTCAGTCG ACATAGTACA AGATGAGGTT GAGTTTAGAG AAAATTCAGA TGACTCAGTA
    CTAAATGTAG TCAAGCATGA AGATACTTCA GATGACTCAA TACAAGATGT AGTCGAGACA
    GGAGAAGTTT CAGATAGCTC AGTACAAGAT GTAGCTGAGT CTGGAGAAAC TTTAGATGAC
    TCTGTTCAAG ATTTTGTTAA AACTGGAGAA ATTTCCATTG ACTCAGTACA CAATTTATTT
    GAGCCTGAAA AAAACGTAGA TGACTCTGTA CAAGATGTAG TTGAGACTGG AGAAGTTTCT
    GTTGATTCAG TACAAGATGT AATTGAGCTT GGAGACACTT TAGATGACTC TGCACAAGGA
    ATAGTTGAAC CTGAAGAAAT GGCAGACAAC TTTATAAAGG AAAGCACTGT AAAGCCAAGA
    CCTGACATCA AAGGGCTTAT TCTACCAACA GAGACAGTGA ACTATGAAAG TGCCACTATG
    AAAGTTCCTG CTCCAGTTAA CCCCAGCATA ACAGAATTTA AGGTTTCATT TGACATCTTC
    CCTTTCGACG ACCCAACTGA TGATGAAGAT AATGGCAGTG GGTTTGCTCA TGGAACTGAC
    TTAGCAAGTT TAGCCTTGCC TGCCAGTCCT GGAAGGGCAT TAATAGTGTT TTTTAGCCTT
    AGGGTCACCA ACATGAAGTT TTCCGAAGAT CTCTTCAACA AGAGCTCTAC TGAATACAAG
    GCACTTGAGC AGCGATTTCT GGAACTGCTC GTACCGTATC TACAGTCCAA CCTCAGCAAT
    TTCCAGAATT TAGAGATTTT GAACTTCAGG AATGGTAGCA TTGTGGTGAA CAGCAGGATG
    AAGTTTGCCA AGCCAGTTCC CCATGGTGTG ACAACAGCAG TGTACCTAAT CTTGGAGGAC
    TTCTGCAACA CTGCCTACCA AACTATGAAT CTTGCAATAG ACAAGTATTC ATTGGATGTC
    GAATCAGGTG ACCAGGCAGA TCCTTGTAAG TTTCAGGCAT GTAACGAGTT CTCCAAGTGT
    TTGGTGAACC GCTGGTCAGG TGAGGCCGAG TGTGTTTGTA ACGCTGGTTA TTTCAGCGTT
    GACGGACTGC CATGCCAGAG CATCTGCGAC ATCCAAGAAG ACTTCTGCCT AAATGACGGG
    AAATGTGACA TAATACCTGG CAAGGGCGCC ATCTGCAGGT GTCGCGTTGG TGAAAACTGG
    TGGTATCGAG GAGAACACTG TGAGGAATAT GTGTCAGAGC CGCTGGTGGT CGGCATTGCC
    ATTGCGTCCG TTGCTGGATT TCTCCTGGTG GCGTCGGGTG TCATCTTTTT CCTGGCCCGA
    ACTCTGAGAG ACCAGTATGA CACAGATGAT TCAGAAGACC CTTTACGATA TGCAGAGAAC
    TTGCCATCTC TAGAAAGAGC CACCAAGTAC AACCCGATGT TTGAAAGTGA AGTGACTGCA
    GGCCATAATC AGTATTATCG GCGTTACCCA GAAGCTCCGG CATTCAGCAC GGGCAGCACC
    GAGATCTCCA CTGACTTCAG CAGCGAAGAG ATTCGTCACA TCTATCAGAA CAGTGAACTT
    ACTAAAGAGG AAATCCAGGA CCGTATTCGT ATTATAGAGC TTTACGCCAA GGACCGCCAG
    TTTGCAGATT TTGTCCGACA GCACCAAGTG GCGCTTGACG CTAGACGAGA GAGCTCTTCC
    AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330159.1
    CDS233..8698
    Translation

    Target ORF information:

    RefSeq Version XM_021474484.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio interphotoreceptor matrix proteoglycan 2b (impg2b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474484.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGCCTGATT TACCTTTTAA ATGCATTTGG TGGGGTTTGA CACTATTGTT GTTGACTGGA 
    TACTTTTCTA TAGATGCAGA TACTGGAACT CATGAGGATT ACTTGGCAAA TGGAAATACA
    ATGCTTTTGG ACACTAATGA AGTTTTGAAT CACTTATTGT TGTTGGACAA AGGTAAAACT
    TTGAATTACA TGTCTGCTGA AGGGAAAACA GCTCTATCTA GACATAAAAG GGACCTCCTG
    TTCCCCAATG GAGTTAAACT GTGCTCACAT GAGACGGTGC AACAGGCAGT GCAGAATCAT
    CTCAACTACT TTCACCTGCG AGTGTGCCAG GAGACAATTT GGGAGGCTTA TAAAATCTTC
    TGGGATCGCT TACCTGAGAA AGAAGAGTAC CAGATCTGGA TGAAAAAATG TCAAAATGGT
    AGCATTAGCG TGTTTGACAT CGGCCGAAGC TTTAGTCAGT CAGCTGAGCA CCTTGCTTTG
    ATTTCAAGTA GGGTAGCATC ATCCACAATA ACATCAACAA GTGCGCCCAC CAGCCCATGG
    CAACCTGAAT GCAGGCATCA GACGACGACT ACTTCGCCAA CACTAGCAAA AGCTGAACAA
    GTTGGTATTT TGATTCCTGA GGCAGTGCCA GGTTCACAAG TTGTCACACC GCAGACCTTT
    GCAGATGATA TCGTAATCAC AACTTTGCCT ACAAGGCTTC AAGTGACACC TGGAATTACC
    CCAAAGCCTG ATCTTGTCAC AGCCTTTAAG GACACAGTAA AACCTATTTC TGAAACTGAA
    CCTGAGGTCA TATTGGGTAC TACTTCTGAG AGTCCTGTTA AAGCATTGGA TACCATTACC
    ATTAAACATG AAGAGGTTGT TGTAGTCTCA GAATCTAAAT TATTGATTAC TGAGAGGACT
    TCGGAGGACT CTACTTCAAG GAAATATGAT TATGAGTTTC CCACGACCTT TAAGGACTCG
    TTAGTAATAA ACACTCCTAT AGTACTAACA ACTGAATTTT CTGAACAAAT GTATTCAGAA
    ATAGGACAAG GTTTTTCTGA TGGTACAGTT AAAACAGTAG CTGTTACTAT TCAAAAGGAA
    CTTGACATTG TCTCAAAATC TACATTATTA ACCACTGACA AAACTCCAGA AGTTTCTACT
    TCAAAAAAAT CTGAGCATGA TAATCCCACT AACTTTGTAG ATTCATTGGG AATAAGCACC
    AATACTCCTA GAGTGACCAC AAGTGAACTC AAAGAAGATC TTCATTCAGA ATTAGAACAG
    GTGGCTGGGC AGGATGTTAT TCACAGTCCT GTTAAAACAA CACATGTTAC AATTAAGCAT
    GAAGTGTTGG ACACAAATAC TACATTAATG ACAACTGACA GAACTCCATA TTCTACTTCA
    GCAAAATATG AACGTGTTAT ACCAACATCC TTTGAGGACT CACTAGTCAC AAGTTCATCT
    ACTGAACAGG ATATGACCAA TGTTCCTATT AAGACGGCAT ATGCCACAAT TTCACATGGA
    GAGTCTGAAA TTGTCACAGA GTCGACAATA ACAACCACTA ACAGAAATCT TGAAGATTCT
    TCTTTAGCAA AATATGATTA TGGGATTCCT CAAGGAATTG ATACTTCTTC GGGAGTAATT
    AATGAATCTT CTACAAAAGA ACATAATTCA GTATCTAAAT TGAATATTGG TCAGACTACT
    ACTGAAGGTC CCCAAACAAC TGATGCAGTT CAGTATGTAG AATCTGACAT TGTGTCAGAG
    GAAACGTTGA AATCCACTGG CAGAACTGCA GTTAGCTCAA ACTCTACAAA AATTGAGGCC
    GATCTTTCCA CAACTCCTGA GGACCTGACA GGAGTAAGTA CCAACACTCC TGAAATAGTC
    ACTAGAGATT CCATTACAAT ACTTATCCCA GAAACCGAGG GCATTACTAA AAATCCTGTT
    AAATCTGCAC CTATTACCAT TCAGCATGTG GATGTTGATG ATGTTTTAGC AGCTACAGTG
    ATGTCCACTC ATAAACCTCC CAAAGTCTCA CCTACAGAAA TATCCCCCGA TCAGAATCTC
    TTACCAGAGG AATCAAAGCT GCCAACTCCT ACAAAACTAG CAGAAGTTTT GTCTGCCACA
    GAAGAGTTGC CAAAGGATCA GACAGACAAG GATGTTGCTA AAATTGTATA TGTGTCTACT
    GTGCCAGAAC TGGTGTCACC AACTATTCCC GCATATACGT CAACTGACAC TACTAAGGAA
    AACCTACATG TGTTAACAGG GGGGGCAGTT GTTACATCAC TTGATAAAGA TGAGGAAGAA
    CATACACCCC ACACAAAAGA GAGCAAAATT ACACAGGTGA CAACAGGAGC AGAATTACCA
    GGTACCATAA CAGAGAGTAC TCATGGAATG TGGAGGGAAA CCCAAGAGCC AATAAATGTC
    ACACCTGATG CTGAAGACAA TCATTTGGGC AAAGAAGGGT ACATAATAAC AGAGATCCCA
    GAAGTTGCTG AAGTACCAGC TGGAGTGACA CCCAAAATGA TTGTGGAAAC TACAACAGCC
    ACTGAATCTG TGTTTACTGT GAAAGACACA TCTATAATTG TAGACAAAAC TGGCATTGTG
    AAACCTGTCG TGACTACGAC AGAGGCAGCT GAACTTACTG AGGTTGGATT CGAAGATTTA
    GGCACAGACA AAGTAACAAT TCTTGAAAAC ACACTTGAAG ATTCTGTAGG ACTAACAAAT
    GAACCTTTTG CTGTATCATG GGGTGTAACT GAAGCCACTA TTTTAGAGGA ATCATATGTG
    GAAAGTACTG AAAGTATTTC AGGTAAAGAG AAGTCCACTG AAACTATTAA GATCACAGGT
    GAAGCCACAA CTGATGACAT TGGTACTTCT GAAACTGACA TTGTAGTGCC TGTAACCGAT
    CGCATAGAAG ATAATGTAAA TGTATCACCT AAAAATGATG ATGCTAAGGA AACAAACATT
    TTTGAAGACA CAACACCAGA TCTTAGTGTT ACTAATTCTG AAAAAGACAT ACTGACAACT
    ACCATACATA AAGACATGCA TGAAGTTACA GAACCATTAA ATATTCCAGA GGTCACACAT
    AAGGTTGTGA TAGAGATCAC AGATGATGTA CGTGAGACCA GAGGAGATTC CTTACCCGAG
    ACACCAGAAG TCACAACAGA GACACCTAAG GTTGCAACAG AAGCTGATGT CAAAACAACA
    ACAAATGTTA TATCTAAAGC ACCTGAAGTT ATACTGAAGA CAACTGTAAC CAAGTCAGAT
    ACACCTGATG TCACAACTCA GACACCTTTG TTAAAGCTTG AAGATACAGA GAGGGTTGAA
    ATAGTACCTG AAACTCCTGA CATACAAGTG GTTCAAACAG AACACTCAGA AAATGATATC
    CCAACTCCTG TCTTGAAAGT GGAGGATTTC ACAGTTGCGC TGAATGACTT GACAACTCCT
    ATTCAAAAGA CTTCTTCAAC CACATTACAA CAAGACATTT CTAATGATAT TTTAGAAGAA
    AACAATGTGA TGGGAAATGA AATTGATGAT ATTGTGGTTC GACCAGTACG CCCTGTCGGC
    GATCATATTT TGGAGCTAAG TATTAAGCTA AGAGGGGAAA GCTATGACGA TGCTCTGAGG
    GATCCATCAA GTAACTACTA TCAACATCTG TCTACACTTT TTGTTGAAAA GATTGAAGAA
    GTTTTCGAAA GGCTTCCAGG TTTCAAGAAA ATATATGTTC TTGAATTCAG GCCACAGAAG
    GACATTGAAG GAGGTCTGGC AGTGGTGGTG CACTATGCTG TTGTTCTTGA GGGTGATGGG
    TCAGGAGTAA GTAATGAGAC CATGGATTAC ATAAACCTGC ATTCCAACAT GGTTGAGAAC
    TCCTTCACAG ATCCTGAGGA GCTGCCCACT GTGGTCTACA CCATCACTGA CTTCCGAAAC
    TACATCTCCG AAGCCCTTCA CAAGGATAAC TTTATCAGAA ACGCTACTCT GAATGTGGAC
    ATTGATTCTT TGCAACTTGA AAATGTGGAG ACATTGCTGC CTTCCAAACC AACCAGCAGA
    CCGTTTGACT CCAGTGACAT GATGGACAAC GTTCTTGCTT CCGAGAAACC ACCAGATGTT
    CCTGGGCAAG AATTATCCAA CAATGACATC TTCATTACAA ATTATGACAT CTTTGATCCT
    GATCCATGGA CAGGTGGACA AAGTGAAGAC AGAAATGCAA ATGATGTAAT CATCTTTGAG
    GAAAGTCCCA CTCAGTCTCC TGTTGAAGTG TCTTTGAAGA ACTTGGACAT TGAAGTTACT
    TCAAAATCTG AAACTTCAAG CAGTGCCCCT GTTCCTGATG AAGCTGTTAT TGAAGAGGAG
    GGATTTTTAC AGACTGCAAC TACAGTCAGT CCTATTATAG ACACAATAAC TGAATTTACT
    TTACCTACAG AATCACCTCA CAAGGTAGAA GAGACACCAG TTTCTCCTGT AGAACCTCCT
    GAGGTTGAGG AAACTACCCT GGGTGATTTA ACAGACCTTG GCTCAGGCTC AGGATTCTCT
    GGAGATCACA TAGGCCCTGA CATCTGGCCA TGGTCATCAG AAACATCACA AGAAGTCTTG
    AAGGAAGATA CCAATGATGA GATACACCAA CTTCAGGAAA ATCAGGAATC TATAATAAAA
    CAGCTTGAGC CTTTACATCA AGATGTGACT CCTGAGGAGC CATTCTTGGA TAGGGTTCTT
    GTGACCCAAG ACATTCGTAC CAATCCTCAT TACACAACAA CTGACCAGGC CCCTGTATTT
    TGGACAATGG AGACTTTGAC TGTTGAGCTT TCAATGCAGA CTCAAGTAGC CCCTGAACAT
    TATGATGACT ATTTTCCAGA TGCATCAATC ACTATGGCTA CACTTGTAAC CAATCAACCA
    TTGTTACATG TTCACAATCC ATTAGAAACA AAAGATCTGG ATGCATCACA AAGTCCAGGA
    ACAATAAGTT CAACTGCTGA TAAGGACTAT ACAGAACGAC TTGTTACACC CACTGTGATC
    CAACCAAGTG AAACAGTAGC TGTGGATAAG CATACTACAA CAGACAACAA ACAGAATCTG
    GAAACTATGG GTACATTATC TGCAACACTG GGCTCCACAA TGAAGTCCAC TGAAATCTCT
    TCAACTACCC AATTACCTGT GATCATCAAC AATAATACAG TTTCAGTGGA AGGTCCTGCC
    AATTCTTCAG AAGCTGTTCA GCATTCTGTA AATGAAGGAA TCACAGAGTT ACCTGCTTTG
    GTCTGGCCAG ATATTGAAGG ATCAGATGAA GAATTCAAAG TTTTAGATGA GGACATGGAA
    CTCGTCAAGT TTGTGCCTAC AAAAAGCCCA GTGACTGAAC TCTTAGAAGA AGATTTAGTT
    GGTGATGAAA TATTGGTTGC AACAACAACA ACAACAACAA CTCTATCCTC AACAACAGTA
    GCGACTGTGG TAACAAGTTT AGGTCATTCA GCCTCCCTGA CTCCTGAAAA GGACTCACCT
    TTCACTTGGA TATCACATTC ATCAATAGAT GAGGATGAAC CTGTGCAAGA GTCAACCACA
    GAACCTCTAC CCACCCAATC ACCCACTGCT TCTGTTCTTC CAAAGACAAC TACACAGACA
    TTGATGCACA CATACATAGC AGCTGTTGCA GGGGAGCCTG TTTTATCAGA TAACATGGAT
    AATAAGGACT CAACCACAAT GTCCACCCTT TTAGATGTTT CAAAACATTT AAAGACCCTT
    AGTTCCACCT CTGTACAATC ATTTTCAGAA CCAACATTTA CACCTACTGT CCACATAATT
    AAGCATGGAG AAACTTCAGA TGACTCAGTA CAAGATGTAG TTGAGACTGG AGAAGTTTTA
    GATGACTCTG TACAAGATGT ACTTGAGCAT GAAGACATTT CATATGGCTC TGTACAAGAT
    GTAGTTGTAT CTGGAGAAAC TTCAAATGAC TCAGTACAAG ATGTAGTTGA GACTGGAGAA
    GTTTTAGATG ATTCTGTACA AGATGTACTT GAGCATGAGG ACATTTTATC TGGCCCAGTT
    CAAGATGTAG TTGTGTCTGG AGAAACTGCA GAAGACTCAG TACAAGATTT AGTTGAGACT
    AGAGAAGTTT TAGATGATTC TGTACAAGAT GTACTTGAGC ATGAGGACAT TTTATCTGGC
    CCAGTTCAAG ATGTAGTTGT GTCTGGAGAA ACTGCAGATG ACTCAGTACA AGATTTAGTT
    GAGACTGGAG AAGTTTTAGA TGATTCCGTA CAAGATGTAC TTGAGCATGA AGTAATTTCA
    TTTGGCCCAG TACAAGATGT AGTTGTATCT GGAAAAACTT CAAATGACTC AGTACAAGAT
    GTAGTTGAGA GTGCAAAAGA TTCAGATGCC CCAATACAAG ATGTATTTGA GCATAAAGAC
    ATTTCAGTTG ACTCTGTACA AGATGTAGTT GTGTCTGGAG AAACTGCAGA TGACTCTATT
    CAAGATTTAT TTAAGCATGA AGGCACTTCG TTCGACACAG TACAAGATGA GGTTGAGTCT
    AGAGAAACTT CAGATGACTC AGTACAAAAT GTAGTTGAGC ATGAAGACTC TTCAGATGAC
    GCAGTTCAAG ATGTAGTCGA GTCTGGAGAC ACTTCAGATG ACTCTGTACA AGATATATTT
    GAGTCTGCAG AAACTTCAGA TGACTCTGTA CAAGATGTAG CTGAGACTGG AAAAGAATCA
    GATGACTTAG TACAAGATAT ATTTGAGAAT AAAGACATTT CAGTTGACTC AGTACAAGAT
    GTAGTTGTAT CTGGAGAGAC TTCAGATGAC TCTATTCAAG GTTTACTTGA GCATGAAGGC
    ACTTCAGTCG ACATAGTACA AGATGAGGTT GAGTTTAGAG AAAATTCAGA TGACTCAGTA
    CTAAATGTAG TCAAGCATGA AGATACTTCA GATGACTCAA TACAAGATGT AGTCGAGACA
    GGAGAAGTTT CAGATAGCTC AGTACAAGAT GTAGCTGAGT CTGGAGAAAC TTTAGATGAC
    TCTGTTCAAG ATTTTGTTAA AACTGGAGAA ATTTCCATTG ACTCAGTACA CAATTTATTT
    GAGCCTGAAA AAAACGTAGA TGACTCTGTA CAAGATGTAG TTGAGACTGG AGAAGTTTCT
    GTTGATTCAG TACAAGATGT AATTGAGCTT GGAGACACTT TAGATGACTC TGCACAAGGA
    ATAGTTGAAC CTGAAGAAAT GGCAGACAAC TTTATAAAGG AAAGCACTGT AAAGCCAAGA
    CCTGACATCA AAGGGCTTAT TCTACCAACA GAGACAGTGA ACTATGAAAG TGCCACTATG
    AAAGTTCCTG CTCCAGTTAA CCCCAGCATA ACAGAATTTA AGGTTTCATT TGACATCTTC
    CCTTTCGACG ACCCAACTGA TGATGAAGAT AATGGCAGTG GGTTTGCTCA TGGAACTGAC
    TTAGCAAGTT TAGCCTTGCC TGCCAGTCCT GGAAGGGCAT TAATAGTGTT TTTTAGCCTT
    AGGGTCACCA ACATGAAGTT TTCCGAAGAT CTCTTCAACA AGAGCTCTAC TGAATACAAG
    GCACTTGAGC AGCGATTTCT GGAACTGCTC GTACCGTATC TACAGTCCAA CCTCAGCAAT
    TTCCAGAATT TAGAGATTTT GAACTTCAGG AATGGTAGCA TTGTGGTGAA CAGCAGGATG
    AAGTTTGCCA AGCCAGTTCC CCATGGTGTG ACAACAGCAG TGTACCTAAT CTTGGAGGAC
    TTCTGCAACA CTGCCTACCA AACTATGAAT CTTGCAATAG ACAAGTATTC ATTGGATGTC
    GAATCAGGTG ACCAGGCAGA TCCTTGTAAG TTTCAGGCAT GTAACGAGTT CTCCAAGTGT
    TTGGTGAACC GCTGGTCAGG TGAGGCCGAG TGTGTTTGTA ACGCTGGTTA TTTCAGCGTT
    GACGGACTGC CATGCCAGAG CATCTGCGAC ATCCAAGAAG ACTTCTGCCT AAATGACGGG
    AAATGTGACA TAATACCTGG CAAGGGCGCC ATCTGCAGGT GTCGCGTTGG TGAAAACTGG
    TGGTATCGAG GAGAACACTG TGAGGAATAT GTGTCAGAGC CGCTGGTGGT CGGCATTGCC
    ATTGCGTCCG TTGCTGGATT TCTCCTGGTG GCGTCGGGTG TCATCTTTTT CCTGGCCCGA
    ACTCTGAGAG ACCAGTATGA CACAGATGAT TCAGAAGACC CTTTACGATA TGCAGAGAAC
    TTGCCATCTC TAGAAAGAGC CACCAAGTAC AACCCGATGT TTGAAAGTGA AGTGACTGCA
    GGCCATAATC AGTATTATCG GCGTTACCCA GAAGCTCCGG CATTCAGCAC GGGCAGCACC
    GAGATCTCCA CTGACTTCAG CAGCGAAGAG ATTCGTCACA TCTATCAGAA CAGTGAACTT
    ACTAAAGAGG AAATCCAGGA CCGTATTCGT ATTATAGAGC TTTACGCCAA GGACCGCCAG
    TTTGCAGATT TTGTCCGACA GCACCAAGTG GCGCTTGACG CTAGACGAGA GAGCTCTTCC
    AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27099
    Clone ID Related Accession (Same CDS sequence) XM_021474484.1 , XM_009296447.3 , XM_009296447.2 , XM_021473520.1
    Accession Version XM_009296447.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8466bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296447.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGCCTGATT TACCTTTTAA ATGCATTTGG TGGGGTTTGA CACTATTGTT GTTGACTGGA 
    TACTTTTCTA TAGATGCAGA TACTGGAACT CATGAGGATT ACTTGGCAAA TGGAAATACA
    ATGCTTTTGG ACACTAATGA AGTTTTGAAT CACTTATTGT TGTTGGACAA AGGTAAAACT
    TTGAATTACA TGTCTGCTGA AGGGAAAACA GCTCTATCTA GACATAAAAG GGACCTCCTG
    TTCCCCAATG GAGTTAAACT GTGCTCACAT GAGACGGTGC AACAGGCAGT GCAGAATCAT
    CTCAACTACT TTCACCTGCG AGTGTGCCAG GAGACAATTT GGGAGGCTTA TAAAATCTTC
    TGGGATCGCT TACCTGAGAA AGAAGAGTAC CAGATCTGGA TGAAAAAATG TCAAAATGGT
    AGCATTAGCG TGTTTGACAT CGGCCGAAGC TTTAGTCAGT CAGCTGAGCA CCTTGCTTTG
    ATTTCAAGTA GGGTAGCATC ATCCACAATA ACATCAACAA GTGCGCCCAC CAGCCCATGG
    CAACCTGAAT GCAGGCATCA GACGACGACT ACTTCGCCAA CACTAGCAAA AGCTGAACAA
    GTTGGTATTT TGATTCCTGA GGCAGTGCCA GGTTCACAAG TTGTCACACC GCAGACCTTT
    GCAGATGATA TCGTAATCAC AACTTTGCCT ACAAGGCTTC AAGTGACACC TGGAATTACC
    CCAAAGCCTG ATCTTGTCAC AGCCTTTAAG GACACAGTAA AACCTATTTC TGAAACTGAA
    CCTGAGGTCA TATTGGGTAC TACTTCTGAG AGTCCTGTTA AAGCATTGGA TACCATTACC
    ATTAAACATG AAGAGGTTGT TGTAGTCTCA GAATCTAAAT TATTGATTAC TGAGAGGACT
    TCGGAGGACT CTACTTCAAG GAAATATGAT TATGAGTTTC CCACGACCTT TAAGGACTCG
    TTAGTAATAA ACACTCCTAT AGTACTAACA ACTGAATTTT CTGAACAAAT GTATTCAGAA
    ATAGGACAAG GTTTTTCTGA TGGTACAGTT AAAACAGTAG CTGTTACTAT TCAAAAGGAA
    CTTGACATTG TCTCAAAATC TACATTATTA ACCACTGACA AAACTCCAGA AGTTTCTACT
    TCAAAAAAAT CTGAGCATGA TAATCCCACT AACTTTGTAG ATTCATTGGG AATAAGCACC
    AATACTCCTA GAGTGACCAC AAGTGAACTC AAAGAAGATC TTCATTCAGA ATTAGAACAG
    GTGGCTGGGC AGGATGTTAT TCACAGTCCT GTTAAAACAA CACATGTTAC AATTAAGCAT
    GAAGTGTTGG ACACAAATAC TACATTAATG ACAACTGACA GAACTCCATA TTCTACTTCA
    GCAAAATATG AACGTGTTAT ACCAACATCC TTTGAGGACT CACTAGTCAC AAGTTCATCT
    ACTGAACAGG ATATGACCAA TGTTCCTATT AAGACGGCAT ATGCCACAAT TTCACATGGA
    GAGTCTGAAA TTGTCACAGA GTCGACAATA ACAACCACTA ACAGAAATCT TGAAGATTCT
    TCTTTAGCAA AATATGATTA TGGGATTCCT CAAGGAATTG ATACTTCTTC GGGAGTAATT
    AATGAATCTT CTACAAAAGA ACATAATTCA GTATCTAAAT TGAATATTGG TCAGACTACT
    ACTGAAGGTC CCCAAACAAC TGATGCAGTT CAGTATGTAG AATCTGACAT TGTGTCAGAG
    GAAACGTTGA AATCCACTGG CAGAACTGCA GTTAGCTCAA ACTCTACAAA AATTGAGGCC
    GATCTTTCCA CAACTCCTGA GGACCTGACA GGAGTAAGTA CCAACACTCC TGAAATAGTC
    ACTAGAGATT CCATTACAAT ACTTATCCCA GAAACCGAGG GCATTACTAA AAATCCTGTT
    AAATCTGCAC CTATTACCAT TCAGCATGTG GATGTTGATG ATGTTTTAGC AGCTACAGTG
    ATGTCCACTC ATAAACCTCC CAAAGTCTCA CCTACAGAAA TATCCCCCGA TCAGAATCTC
    TTACCAGAGG AATCAAAGCT GCCAACTCCT ACAAAACTAG CAGAAGTTTT GTCTGCCACA
    GAAGAGTTGC CAAAGGATCA GACAGACAAG GATGTTGCTA AAATTGTATA TGTGTCTACT
    GTGCCAGAAC TGGTGTCACC AACTATTCCC GCATATACGT CAACTGACAC TACTAAGGAA
    AACCTACATG TGTTAACAGG GGGGGCAGTT GTTACATCAC TTGATAAAGA TGAGGAAGAA
    CATACACCCC ACACAAAAGA GAGCAAAATT ACACAGGTGA CAACAGGAGC AGAATTACCA
    GGTACCATAA CAGAGAGTAC TCATGGAATG TGGAGGGAAA CCCAAGAGCC AATAAATGTC
    ACACCTGATG CTGAAGACAA TCATTTGGGC AAAGAAGGGT ACATAATAAC AGAGATCCCA
    GAAGTTGCTG AAGTACCAGC TGGAGTGACA CCCAAAATGA TTGTGGAAAC TACAACAGCC
    ACTGAATCTG TGTTTACTGT GAAAGACACA TCTATAATTG TAGACAAAAC TGGCATTGTG
    AAACCTGTCG TGACTACGAC AGAGGCAGCT GAACTTACTG AGGTTGGATT CGAAGATTTA
    GGCACAGACA AAGTAACAAT TCTTGAAAAC ACACTTGAAG ATTCTGTAGG ACTAACAAAT
    GAACCTTTTG CTGTATCATG GGGTGTAACT GAAGCCACTA TTTTAGAGGA ATCATATGTG
    GAAAGTACTG AAAGTATTTC AGGTAAAGAG AAGTCCACTG AAACTATTAA GATCACAGGT
    GAAGCCACAA CTGATGACAT TGGTACTTCT GAAACTGACA TTGTAGTGCC TGTAACCGAT
    CGCATAGAAG ATAATGTAAA TGTATCACCT AAAAATGATG ATGCTAAGGA AACAAACATT
    TTTGAAGACA CAACACCAGA TCTTAGTGTT ACTAATTCTG AAAAAGACAT ACTGACAACT
    ACCATACATA AAGACATGCA TGAAGTTACA GAACCATTAA ATATTCCAGA GGTCACACAT
    AAGGTTGTGA TAGAGATCAC AGATGATGTA CGTGAGACCA GAGGAGATTC CTTACCCGAG
    ACACCAGAAG TCACAACAGA GACACCTAAG GTTGCAACAG AAGCTGATGT CAAAACAACA
    ACAAATGTTA TATCTAAAGC ACCTGAAGTT ATACTGAAGA CAACTGTAAC CAAGTCAGAT
    ACACCTGATG TCACAACTCA GACACCTTTG TTAAAGCTTG AAGATACAGA GAGGGTTGAA
    ATAGTACCTG AAACTCCTGA CATACAAGTG GTTCAAACAG AACACTCAGA AAATGATATC
    CCAACTCCTG TCTTGAAAGT GGAGGATTTC ACAGTTGCGC TGAATGACTT GACAACTCCT
    ATTCAAAAGA CTTCTTCAAC CACATTACAA CAAGACATTT CTAATGATAT TTTAGAAGAA
    AACAATGTGA TGGGAAATGA AATTGATGAT ATTGTGGTTC GACCAGTACG CCCTGTCGGC
    GATCATATTT TGGAGCTAAG TATTAAGCTA AGAGGGGAAA GCTATGACGA TGCTCTGAGG
    GATCCATCAA GTAACTACTA TCAACATCTG TCTACACTTT TTGTTGAAAA GATTGAAGAA
    GTTTTCGAAA GGCTTCCAGG TTTCAAGAAA ATATATGTTC TTGAATTCAG GCCACAGAAG
    GACATTGAAG GAGGTCTGGC AGTGGTGGTG CACTATGCTG TTGTTCTTGA GGGTGATGGG
    TCAGGAGTAA GTAATGAGAC CATGGATTAC ATAAACCTGC ATTCCAACAT GGTTGAGAAC
    TCCTTCACAG ATCCTGAGGA GCTGCCCACT GTGGTCTACA CCATCACTGA CTTCCGAAAC
    TACATCTCCG AAGCCCTTCA CAAGGATAAC TTTATCAGAA ACGCTACTCT GAATGTGGAC
    ATTGATTCTT TGCAACTTGA AAATGTGGAG ACATTGCTGC CTTCCAAACC AACCAGCAGA
    CCGTTTGACT CCAGTGACAT GATGGACAAC GTTCTTGCTT CCGAGAAACC ACCAGATGTT
    CCTGGGCAAG AATTATCCAA CAATGACATC TTCATTACAA ATTATGACAT CTTTGATCCT
    GATCCATGGA CAGGTGGACA AAGTGAAGAC AGAAATGCAA ATGATGTAAT CATCTTTGAG
    GAAAGTCCCA CTCAGTCTCC TGTTGAAGTG TCTTTGAAGA ACTTGGACAT TGAAGTTACT
    TCAAAATCTG AAACTTCAAG CAGTGCCCCT GTTCCTGATG AAGCTGTTAT TGAAGAGGAG
    GGATTTTTAC AGACTGCAAC TACAGTCAGT CCTATTATAG ACACAATAAC TGAATTTACT
    TTACCTACAG AATCACCTCA CAAGGTAGAA GAGACACCAG TTTCTCCTGT AGAACCTCCT
    GAGGTTGAGG AAACTACCCT GGGTGATTTA ACAGACCTTG GCTCAGGCTC AGGATTCTCT
    GGAGATCACA TAGGCCCTGA CATCTGGCCA TGGTCATCAG AAACATCACA AGAAGTCTTG
    AAGGAAGATA CCAATGATGA GATACACCAA CTTCAGGAAA ATCAGGAATC TATAATAAAA
    CAGCTTGAGC CTTTACATCA AGATGTGACT CCTGAGGAGC CATTCTTGGA TAGGGTTCTT
    GTGACCCAAG ACATTCGTAC CAATCCTCAT TACACAACAA CTGACCAGGC CCCTGTATTT
    TGGACAATGG AGACTTTGAC TGTTGAGCTT TCAATGCAGA CTCAAGTAGC CCCTGAACAT
    TATGATGACT ATTTTCCAGA TGCATCAATC ACTATGGCTA CACTTGTAAC CAATCAACCA
    TTGTTACATG TTCACAATCC ATTAGAAACA AAAGATCTGG ATGCATCACA AAGTCCAGGA
    ACAATAAGTT CAACTGCTGA TAAGGACTAT ACAGAACGAC TTGTTACACC CACTGTGATC
    CAACCAAGTG AAACAGTAGC TGTGGATAAG CATACTACAA CAGACAACAA ACAGAATCTG
    GAAACTATGG GTACATTATC TGCAACACTG GGCTCCACAA TGAAGTCCAC TGAAATCTCT
    TCAACTACCC AATTACCTGT GATCATCAAC AATAATACAG TTTCAGTGGA AGGTCCTGCC
    AATTCTTCAG AAGCTGTTCA GCATTCTGTA AATGAAGGAA TCACAGAGTT ACCTGCTTTG
    GTCTGGCCAG ATATTGAAGG ATCAGATGAA GAATTCAAAG TTTTAGATGA GGACATGGAA
    CTCGTCAAGT TTGTGCCTAC AAAAAGCCCA GTGACTGAAC TCTTAGAAGA AGATTTAGTT
    GGTGATGAAA TATTGGTTGC AACAACAACA ACAACAACAA CTCTATCCTC AACAACAGTA
    GCGACTGTGG TAACAAGTTT AGGTCATTCA GCCTCCCTGA CTCCTGAAAA GGACTCACCT
    TTCACTTGGA TATCACATTC ATCAATAGAT GAGGATGAAC CTGTGCAAGA GTCAACCACA
    GAACCTCTAC CCACCCAATC ACCCACTGCT TCTGTTCTTC CAAAGACAAC TACACAGACA
    TTGATGCACA CATACATAGC AGCTGTTGCA GGGGAGCCTG TTTTATCAGA TAACATGGAT
    AATAAGGACT CAACCACAAT GTCCACCCTT TTAGATGTTT CAAAACATTT AAAGACCCTT
    AGTTCCACCT CTGTACAATC ATTTTCAGAA CCAACATTTA CACCTACTGT CCACATAATT
    AAGCATGGAG AAACTTCAGA TGACTCAGTA CAAGATGTAG TTGAGACTGG AGAAGTTTTA
    GATGACTCTG TACAAGATGT ACTTGAGCAT GAAGACATTT CATATGGCTC TGTACAAGAT
    GTAGTTGTAT CTGGAGAAAC TTCAAATGAC TCAGTACAAG ATGTAGTTGA GACTGGAGAA
    GTTTTAGATG ATTCTGTACA AGATGTACTT GAGCATGAGG ACATTTTATC TGGCCCAGTT
    CAAGATGTAG TTGTGTCTGG AGAAACTGCA GAAGACTCAG TACAAGATTT AGTTGAGACT
    AGAGAAGTTT TAGATGATTC TGTACAAGAT GTACTTGAGC ATGAGGACAT TTTATCTGGC
    CCAGTTCAAG ATGTAGTTGT GTCTGGAGAA ACTGCAGATG ACTCAGTACA AGATTTAGTT
    GAGACTGGAG AAGTTTTAGA TGATTCCGTA CAAGATGTAC TTGAGCATGA AGTAATTTCA
    TTTGGCCCAG TACAAGATGT AGTTGTATCT GGAAAAACTT CAAATGACTC AGTACAAGAT
    GTAGTTGAGA GTGCAAAAGA TTCAGATGCC CCAATACAAG ATGTATTTGA GCATAAAGAC
    ATTTCAGTTG ACTCTGTACA AGATGTAGTT GTGTCTGGAG AAACTGCAGA TGACTCTATT
    CAAGATTTAT TTAAGCATGA AGGCACTTCG TTCGACACAG TACAAGATGA GGTTGAGTCT
    AGAGAAACTT CAGATGACTC AGTACAAAAT GTAGTTGAGC ATGAAGACTC TTCAGATGAC
    GCAGTTCAAG ATGTAGTCGA GTCTGGAGAC ACTTCAGATG ACTCTGTACA AGATATATTT
    GAGTCTGCAG AAACTTCAGA TGACTCTGTA CAAGATGTAG CTGAGACTGG AAAAGAATCA
    GATGACTTAG TACAAGATAT ATTTGAGAAT AAAGACATTT CAGTTGACTC AGTACAAGAT
    GTAGTTGTAT CTGGAGAGAC TTCAGATGAC TCTATTCAAG GTTTACTTGA GCATGAAGGC
    ACTTCAGTCG ACATAGTACA AGATGAGGTT GAGTTTAGAG AAAATTCAGA TGACTCAGTA
    CTAAATGTAG TCAAGCATGA AGATACTTCA GATGACTCAA TACAAGATGT AGTCGAGACA
    GGAGAAGTTT CAGATAGCTC AGTACAAGAT GTAGCTGAGT CTGGAGAAAC TTTAGATGAC
    TCTGTTCAAG ATTTTGTTAA AACTGGAGAA ATTTCCATTG ACTCAGTACA CAATTTATTT
    GAGCCTGAAA AAAACGTAGA TGACTCTGTA CAAGATGTAG TTGAGACTGG AGAAGTTTCT
    GTTGATTCAG TACAAGATGT AATTGAGCTT GGAGACACTT TAGATGACTC TGCACAAGGA
    ATAGTTGAAC CTGAAGAAAT GGCAGACAAC TTTATAAAGG AAAGCACTGT AAAGCCAAGA
    CCTGACATCA AAGGGCTTAT TCTACCAACA GAGACAGTGA ACTATGAAAG TGCCACTATG
    AAAGTTCCTG CTCCAGTTAA CCCCAGCATA ACAGAATTTA AGGTTTCATT TGACATCTTC
    CCTTTCGACG ACCCAACTGA TGATGAAGAT AATGGCAGTG GGTTTGCTCA TGGAACTGAC
    TTAGCAAGTT TAGCCTTGCC TGCCAGTCCT GGAAGGGCAT TAATAGTGTT TTTTAGCCTT
    AGGGTCACCA ACATGAAGTT TTCCGAAGAT CTCTTCAACA AGAGCTCTAC TGAATACAAG
    GCACTTGAGC AGCGATTTCT GGAACTGCTC GTACCGTATC TACAGTCCAA CCTCAGCAAT
    TTCCAGAATT TAGAGATTTT GAACTTCAGG AATGGTAGCA TTGTGGTGAA CAGCAGGATG
    AAGTTTGCCA AGCCAGTTCC CCATGGTGTG ACAACAGCAG TGTACCTAAT CTTGGAGGAC
    TTCTGCAACA CTGCCTACCA AACTATGAAT CTTGCAATAG ACAAGTATTC ATTGGATGTC
    GAATCAGGTG ACCAGGCAGA TCCTTGTAAG TTTCAGGCAT GTAACGAGTT CTCCAAGTGT
    TTGGTGAACC GCTGGTCAGG TGAGGCCGAG TGTGTTTGTA ACGCTGGTTA TTTCAGCGTT
    GACGGACTGC CATGCCAGAG CATCTGCGAC ATCCAAGAAG ACTTCTGCCT AAATGACGGG
    AAATGTGACA TAATACCTGG CAAGGGCGCC ATCTGCAGGT GTCGCGTTGG TGAAAACTGG
    TGGTATCGAG GAGAACACTG TGAGGAATAT GTGTCAGAGC CGCTGGTGGT CGGCATTGCC
    ATTGCGTCCG TTGCTGGATT TCTCCTGGTG GCGTCGGGTG TCATCTTTTT CCTGGCCCGA
    ACTCTGAGAG ACCAGTATGA CACAGATGAT TCAGAAGACC CTTTACGATA TGCAGAGAAC
    TTGCCATCTC TAGAAAGAGC CACCAAGTAC AACCCGATGT TTGAAAGTGA AGTGACTGCA
    GGCCATAATC AGTATTATCG GCGTTACCCA GAAGCTCCGG CATTCAGCAC GGGCAGCACC
    GAGATCTCCA CTGACTTCAG CAGCGAAGAG ATTCGTCACA TCTATCAGAA CAGTGAACTT
    ACTAAAGAGG AAATCCAGGA CCGTATTCGT ATTATAGAGC TTTACGCCAA GGACCGCCAG
    TTTGCAGATT TTGTCCGACA GCACCAAGTG GCGCTTGACG CTAGACGAGA GAGCTCTTCC
    AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009294722.1
    CDS233..8698
    Translation

    Target ORF information:

    RefSeq Version XM_009296447.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio interphotoreceptor matrix proteoglycan 2b (impg2b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296447.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGCCTGATT TACCTTTTAA ATGCATTTGG TGGGGTTTGA CACTATTGTT GTTGACTGGA 
    TACTTTTCTA TAGATGCAGA TACTGGAACT CATGAGGATT ACTTGGCAAA TGGAAATACA
    ATGCTTTTGG ACACTAATGA AGTTTTGAAT CACTTATTGT TGTTGGACAA AGGTAAAACT
    TTGAATTACA TGTCTGCTGA AGGGAAAACA GCTCTATCTA GACATAAAAG GGACCTCCTG
    TTCCCCAATG GAGTTAAACT GTGCTCACAT GAGACGGTGC AACAGGCAGT GCAGAATCAT
    CTCAACTACT TTCACCTGCG AGTGTGCCAG GAGACAATTT GGGAGGCTTA TAAAATCTTC
    TGGGATCGCT TACCTGAGAA AGAAGAGTAC CAGATCTGGA TGAAAAAATG TCAAAATGGT
    AGCATTAGCG TGTTTGACAT CGGCCGAAGC TTTAGTCAGT CAGCTGAGCA CCTTGCTTTG
    ATTTCAAGTA GGGTAGCATC ATCCACAATA ACATCAACAA GTGCGCCCAC CAGCCCATGG
    CAACCTGAAT GCAGGCATCA GACGACGACT ACTTCGCCAA CACTAGCAAA AGCTGAACAA
    GTTGGTATTT TGATTCCTGA GGCAGTGCCA GGTTCACAAG TTGTCACACC GCAGACCTTT
    GCAGATGATA TCGTAATCAC AACTTTGCCT ACAAGGCTTC AAGTGACACC TGGAATTACC
    CCAAAGCCTG ATCTTGTCAC AGCCTTTAAG GACACAGTAA AACCTATTTC TGAAACTGAA
    CCTGAGGTCA TATTGGGTAC TACTTCTGAG AGTCCTGTTA AAGCATTGGA TACCATTACC
    ATTAAACATG AAGAGGTTGT TGTAGTCTCA GAATCTAAAT TATTGATTAC TGAGAGGACT
    TCGGAGGACT CTACTTCAAG GAAATATGAT TATGAGTTTC CCACGACCTT TAAGGACTCG
    TTAGTAATAA ACACTCCTAT AGTACTAACA ACTGAATTTT CTGAACAAAT GTATTCAGAA
    ATAGGACAAG GTTTTTCTGA TGGTACAGTT AAAACAGTAG CTGTTACTAT TCAAAAGGAA
    CTTGACATTG TCTCAAAATC TACATTATTA ACCACTGACA AAACTCCAGA AGTTTCTACT
    TCAAAAAAAT CTGAGCATGA TAATCCCACT AACTTTGTAG ATTCATTGGG AATAAGCACC
    AATACTCCTA GAGTGACCAC AAGTGAACTC AAAGAAGATC TTCATTCAGA ATTAGAACAG
    GTGGCTGGGC AGGATGTTAT TCACAGTCCT GTTAAAACAA CACATGTTAC AATTAAGCAT
    GAAGTGTTGG ACACAAATAC TACATTAATG ACAACTGACA GAACTCCATA TTCTACTTCA
    GCAAAATATG AACGTGTTAT ACCAACATCC TTTGAGGACT CACTAGTCAC AAGTTCATCT
    ACTGAACAGG ATATGACCAA TGTTCCTATT AAGACGGCAT ATGCCACAAT TTCACATGGA
    GAGTCTGAAA TTGTCACAGA GTCGACAATA ACAACCACTA ACAGAAATCT TGAAGATTCT
    TCTTTAGCAA AATATGATTA TGGGATTCCT CAAGGAATTG ATACTTCTTC GGGAGTAATT
    AATGAATCTT CTACAAAAGA ACATAATTCA GTATCTAAAT TGAATATTGG TCAGACTACT
    ACTGAAGGTC CCCAAACAAC TGATGCAGTT CAGTATGTAG AATCTGACAT TGTGTCAGAG
    GAAACGTTGA AATCCACTGG CAGAACTGCA GTTAGCTCAA ACTCTACAAA AATTGAGGCC
    GATCTTTCCA CAACTCCTGA GGACCTGACA GGAGTAAGTA CCAACACTCC TGAAATAGTC
    ACTAGAGATT CCATTACAAT ACTTATCCCA GAAACCGAGG GCATTACTAA AAATCCTGTT
    AAATCTGCAC CTATTACCAT TCAGCATGTG GATGTTGATG ATGTTTTAGC AGCTACAGTG
    ATGTCCACTC ATAAACCTCC CAAAGTCTCA CCTACAGAAA TATCCCCCGA TCAGAATCTC
    TTACCAGAGG AATCAAAGCT GCCAACTCCT ACAAAACTAG CAGAAGTTTT GTCTGCCACA
    GAAGAGTTGC CAAAGGATCA GACAGACAAG GATGTTGCTA AAATTGTATA TGTGTCTACT
    GTGCCAGAAC TGGTGTCACC AACTATTCCC GCATATACGT CAACTGACAC TACTAAGGAA
    AACCTACATG TGTTAACAGG GGGGGCAGTT GTTACATCAC TTGATAAAGA TGAGGAAGAA
    CATACACCCC ACACAAAAGA GAGCAAAATT ACACAGGTGA CAACAGGAGC AGAATTACCA
    GGTACCATAA CAGAGAGTAC TCATGGAATG TGGAGGGAAA CCCAAGAGCC AATAAATGTC
    ACACCTGATG CTGAAGACAA TCATTTGGGC AAAGAAGGGT ACATAATAAC AGAGATCCCA
    GAAGTTGCTG AAGTACCAGC TGGAGTGACA CCCAAAATGA TTGTGGAAAC TACAACAGCC
    ACTGAATCTG TGTTTACTGT GAAAGACACA TCTATAATTG TAGACAAAAC TGGCATTGTG
    AAACCTGTCG TGACTACGAC AGAGGCAGCT GAACTTACTG AGGTTGGATT CGAAGATTTA
    GGCACAGACA AAGTAACAAT TCTTGAAAAC ACACTTGAAG ATTCTGTAGG ACTAACAAAT
    GAACCTTTTG CTGTATCATG GGGTGTAACT GAAGCCACTA TTTTAGAGGA ATCATATGTG
    GAAAGTACTG AAAGTATTTC AGGTAAAGAG AAGTCCACTG AAACTATTAA GATCACAGGT
    GAAGCCACAA CTGATGACAT TGGTACTTCT GAAACTGACA TTGTAGTGCC TGTAACCGAT
    CGCATAGAAG ATAATGTAAA TGTATCACCT AAAAATGATG ATGCTAAGGA AACAAACATT
    TTTGAAGACA CAACACCAGA TCTTAGTGTT ACTAATTCTG AAAAAGACAT ACTGACAACT
    ACCATACATA AAGACATGCA TGAAGTTACA GAACCATTAA ATATTCCAGA GGTCACACAT
    AAGGTTGTGA TAGAGATCAC AGATGATGTA CGTGAGACCA GAGGAGATTC CTTACCCGAG
    ACACCAGAAG TCACAACAGA GACACCTAAG GTTGCAACAG AAGCTGATGT CAAAACAACA
    ACAAATGTTA TATCTAAAGC ACCTGAAGTT ATACTGAAGA CAACTGTAAC CAAGTCAGAT
    ACACCTGATG TCACAACTCA GACACCTTTG TTAAAGCTTG AAGATACAGA GAGGGTTGAA
    ATAGTACCTG AAACTCCTGA CATACAAGTG GTTCAAACAG AACACTCAGA AAATGATATC
    CCAACTCCTG TCTTGAAAGT GGAGGATTTC ACAGTTGCGC TGAATGACTT GACAACTCCT
    ATTCAAAAGA CTTCTTCAAC CACATTACAA CAAGACATTT CTAATGATAT TTTAGAAGAA
    AACAATGTGA TGGGAAATGA AATTGATGAT ATTGTGGTTC GACCAGTACG CCCTGTCGGC
    GATCATATTT TGGAGCTAAG TATTAAGCTA AGAGGGGAAA GCTATGACGA TGCTCTGAGG
    GATCCATCAA GTAACTACTA TCAACATCTG TCTACACTTT TTGTTGAAAA GATTGAAGAA
    GTTTTCGAAA GGCTTCCAGG TTTCAAGAAA ATATATGTTC TTGAATTCAG GCCACAGAAG
    GACATTGAAG GAGGTCTGGC AGTGGTGGTG CACTATGCTG TTGTTCTTGA GGGTGATGGG
    TCAGGAGTAA GTAATGAGAC CATGGATTAC ATAAACCTGC ATTCCAACAT GGTTGAGAAC
    TCCTTCACAG ATCCTGAGGA GCTGCCCACT GTGGTCTACA CCATCACTGA CTTCCGAAAC
    TACATCTCCG AAGCCCTTCA CAAGGATAAC TTTATCAGAA ACGCTACTCT GAATGTGGAC
    ATTGATTCTT TGCAACTTGA AAATGTGGAG ACATTGCTGC CTTCCAAACC AACCAGCAGA
    CCGTTTGACT CCAGTGACAT GATGGACAAC GTTCTTGCTT CCGAGAAACC ACCAGATGTT
    CCTGGGCAAG AATTATCCAA CAATGACATC TTCATTACAA ATTATGACAT CTTTGATCCT
    GATCCATGGA CAGGTGGACA AAGTGAAGAC AGAAATGCAA ATGATGTAAT CATCTTTGAG
    GAAAGTCCCA CTCAGTCTCC TGTTGAAGTG TCTTTGAAGA ACTTGGACAT TGAAGTTACT
    TCAAAATCTG AAACTTCAAG CAGTGCCCCT GTTCCTGATG AAGCTGTTAT TGAAGAGGAG
    GGATTTTTAC AGACTGCAAC TACAGTCAGT CCTATTATAG ACACAATAAC TGAATTTACT
    TTACCTACAG AATCACCTCA CAAGGTAGAA GAGACACCAG TTTCTCCTGT AGAACCTCCT
    GAGGTTGAGG AAACTACCCT GGGTGATTTA ACAGACCTTG GCTCAGGCTC AGGATTCTCT
    GGAGATCACA TAGGCCCTGA CATCTGGCCA TGGTCATCAG AAACATCACA AGAAGTCTTG
    AAGGAAGATA CCAATGATGA GATACACCAA CTTCAGGAAA ATCAGGAATC TATAATAAAA
    CAGCTTGAGC CTTTACATCA AGATGTGACT CCTGAGGAGC CATTCTTGGA TAGGGTTCTT
    GTGACCCAAG ACATTCGTAC CAATCCTCAT TACACAACAA CTGACCAGGC CCCTGTATTT
    TGGACAATGG AGACTTTGAC TGTTGAGCTT TCAATGCAGA CTCAAGTAGC CCCTGAACAT
    TATGATGACT ATTTTCCAGA TGCATCAATC ACTATGGCTA CACTTGTAAC CAATCAACCA
    TTGTTACATG TTCACAATCC ATTAGAAACA AAAGATCTGG ATGCATCACA AAGTCCAGGA
    ACAATAAGTT CAACTGCTGA TAAGGACTAT ACAGAACGAC TTGTTACACC CACTGTGATC
    CAACCAAGTG AAACAGTAGC TGTGGATAAG CATACTACAA CAGACAACAA ACAGAATCTG
    GAAACTATGG GTACATTATC TGCAACACTG GGCTCCACAA TGAAGTCCAC TGAAATCTCT
    TCAACTACCC AATTACCTGT GATCATCAAC AATAATACAG TTTCAGTGGA AGGTCCTGCC
    AATTCTTCAG AAGCTGTTCA GCATTCTGTA AATGAAGGAA TCACAGAGTT ACCTGCTTTG
    GTCTGGCCAG ATATTGAAGG ATCAGATGAA GAATTCAAAG TTTTAGATGA GGACATGGAA
    CTCGTCAAGT TTGTGCCTAC AAAAAGCCCA GTGACTGAAC TCTTAGAAGA AGATTTAGTT
    GGTGATGAAA TATTGGTTGC AACAACAACA ACAACAACAA CTCTATCCTC AACAACAGTA
    GCGACTGTGG TAACAAGTTT AGGTCATTCA GCCTCCCTGA CTCCTGAAAA GGACTCACCT
    TTCACTTGGA TATCACATTC ATCAATAGAT GAGGATGAAC CTGTGCAAGA GTCAACCACA
    GAACCTCTAC CCACCCAATC ACCCACTGCT TCTGTTCTTC CAAAGACAAC TACACAGACA
    TTGATGCACA CATACATAGC AGCTGTTGCA GGGGAGCCTG TTTTATCAGA TAACATGGAT
    AATAAGGACT CAACCACAAT GTCCACCCTT TTAGATGTTT CAAAACATTT AAAGACCCTT
    AGTTCCACCT CTGTACAATC ATTTTCAGAA CCAACATTTA CACCTACTGT CCACATAATT
    AAGCATGGAG AAACTTCAGA TGACTCAGTA CAAGATGTAG TTGAGACTGG AGAAGTTTTA
    GATGACTCTG TACAAGATGT ACTTGAGCAT GAAGACATTT CATATGGCTC TGTACAAGAT
    GTAGTTGTAT CTGGAGAAAC TTCAAATGAC TCAGTACAAG ATGTAGTTGA GACTGGAGAA
    GTTTTAGATG ATTCTGTACA AGATGTACTT GAGCATGAGG ACATTTTATC TGGCCCAGTT
    CAAGATGTAG TTGTGTCTGG AGAAACTGCA GAAGACTCAG TACAAGATTT AGTTGAGACT
    AGAGAAGTTT TAGATGATTC TGTACAAGAT GTACTTGAGC ATGAGGACAT TTTATCTGGC
    CCAGTTCAAG ATGTAGTTGT GTCTGGAGAA ACTGCAGATG ACTCAGTACA AGATTTAGTT
    GAGACTGGAG AAGTTTTAGA TGATTCCGTA CAAGATGTAC TTGAGCATGA AGTAATTTCA
    TTTGGCCCAG TACAAGATGT AGTTGTATCT GGAAAAACTT CAAATGACTC AGTACAAGAT
    GTAGTTGAGA GTGCAAAAGA TTCAGATGCC CCAATACAAG ATGTATTTGA GCATAAAGAC
    ATTTCAGTTG ACTCTGTACA AGATGTAGTT GTGTCTGGAG AAACTGCAGA TGACTCTATT
    CAAGATTTAT TTAAGCATGA AGGCACTTCG TTCGACACAG TACAAGATGA GGTTGAGTCT
    AGAGAAACTT CAGATGACTC AGTACAAAAT GTAGTTGAGC ATGAAGACTC TTCAGATGAC
    GCAGTTCAAG ATGTAGTCGA GTCTGGAGAC ACTTCAGATG ACTCTGTACA AGATATATTT
    GAGTCTGCAG AAACTTCAGA TGACTCTGTA CAAGATGTAG CTGAGACTGG AAAAGAATCA
    GATGACTTAG TACAAGATAT ATTTGAGAAT AAAGACATTT CAGTTGACTC AGTACAAGAT
    GTAGTTGTAT CTGGAGAGAC TTCAGATGAC TCTATTCAAG GTTTACTTGA GCATGAAGGC
    ACTTCAGTCG ACATAGTACA AGATGAGGTT GAGTTTAGAG AAAATTCAGA TGACTCAGTA
    CTAAATGTAG TCAAGCATGA AGATACTTCA GATGACTCAA TACAAGATGT AGTCGAGACA
    GGAGAAGTTT CAGATAGCTC AGTACAAGAT GTAGCTGAGT CTGGAGAAAC TTTAGATGAC
    TCTGTTCAAG ATTTTGTTAA AACTGGAGAA ATTTCCATTG ACTCAGTACA CAATTTATTT
    GAGCCTGAAA AAAACGTAGA TGACTCTGTA CAAGATGTAG TTGAGACTGG AGAAGTTTCT
    GTTGATTCAG TACAAGATGT AATTGAGCTT GGAGACACTT TAGATGACTC TGCACAAGGA
    ATAGTTGAAC CTGAAGAAAT GGCAGACAAC TTTATAAAGG AAAGCACTGT AAAGCCAAGA
    CCTGACATCA AAGGGCTTAT TCTACCAACA GAGACAGTGA ACTATGAAAG TGCCACTATG
    AAAGTTCCTG CTCCAGTTAA CCCCAGCATA ACAGAATTTA AGGTTTCATT TGACATCTTC
    CCTTTCGACG ACCCAACTGA TGATGAAGAT AATGGCAGTG GGTTTGCTCA TGGAACTGAC
    TTAGCAAGTT TAGCCTTGCC TGCCAGTCCT GGAAGGGCAT TAATAGTGTT TTTTAGCCTT
    AGGGTCACCA ACATGAAGTT TTCCGAAGAT CTCTTCAACA AGAGCTCTAC TGAATACAAG
    GCACTTGAGC AGCGATTTCT GGAACTGCTC GTACCGTATC TACAGTCCAA CCTCAGCAAT
    TTCCAGAATT TAGAGATTTT GAACTTCAGG AATGGTAGCA TTGTGGTGAA CAGCAGGATG
    AAGTTTGCCA AGCCAGTTCC CCATGGTGTG ACAACAGCAG TGTACCTAAT CTTGGAGGAC
    TTCTGCAACA CTGCCTACCA AACTATGAAT CTTGCAATAG ACAAGTATTC ATTGGATGTC
    GAATCAGGTG ACCAGGCAGA TCCTTGTAAG TTTCAGGCAT GTAACGAGTT CTCCAAGTGT
    TTGGTGAACC GCTGGTCAGG TGAGGCCGAG TGTGTTTGTA ACGCTGGTTA TTTCAGCGTT
    GACGGACTGC CATGCCAGAG CATCTGCGAC ATCCAAGAAG ACTTCTGCCT AAATGACGGG
    AAATGTGACA TAATACCTGG CAAGGGCGCC ATCTGCAGGT GTCGCGTTGG TGAAAACTGG
    TGGTATCGAG GAGAACACTG TGAGGAATAT GTGTCAGAGC CGCTGGTGGT CGGCATTGCC
    ATTGCGTCCG TTGCTGGATT TCTCCTGGTG GCGTCGGGTG TCATCTTTTT CCTGGCCCGA
    ACTCTGAGAG ACCAGTATGA CACAGATGAT TCAGAAGACC CTTTACGATA TGCAGAGAAC
    TTGCCATCTC TAGAAAGAGC CACCAAGTAC AACCCGATGT TTGAAAGTGA AGTGACTGCA
    GGCCATAATC AGTATTATCG GCGTTACCCA GAAGCTCCGG CATTCAGCAC GGGCAGCACC
    GAGATCTCCA CTGACTTCAG CAGCGAAGAG ATTCGTCACA TCTATCAGAA CAGTGAACTT
    ACTAAAGAGG AAATCCAGGA CCGTATTCGT ATTATAGAGC TTTACGCCAA GGACCGCCAG
    TTTGCAGATT TTGTCCGACA GCACCAAGTG GCGCTTGACG CTAGACGAGA GAGCTCTTCC
    AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27099
    Clone ID Related Accession (Same CDS sequence) XM_021474484.1 , XM_009296447.3 , XM_009296447.2 , XM_021473520.1
    Accession Version XM_009296447.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8466bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product titin homolog isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296447.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGCCTGATT TACCTTTTAA ATGCATTTGG TGGGGTTTGA CACTATTGTT GTTGACTGGA 
    TACTTTTCTA TAGATGCAGA TACTGGAACT CATGAGGATT ACTTGGCAAA TGGAAATACA
    ATGCTTTTGG ACACTAATGA AGTTTTGAAT CACTTATTGT TGTTGGACAA AGGTAAAACT
    TTGAATTACA TGTCTGCTGA AGGGAAAACA GCTCTATCTA GACATAAAAG GGACCTCCTG
    TTCCCCAATG GAGTTAAACT GTGCTCACAT GAGACGGTGC AACAGGCAGT GCAGAATCAT
    CTCAACTACT TTCACCTGCG AGTGTGCCAG GAGACAATTT GGGAGGCTTA TAAAATCTTC
    TGGGATCGCT TACCTGAGAA AGAAGAGTAC CAGATCTGGA TGAAAAAATG TCAAAATGGT
    AGCATTAGCG TGTTTGACAT CGGCCGAAGC TTTAGTCAGT CAGCTGAGCA CCTTGCTTTG
    ATTTCAAGTA GGGTAGCATC ATCCACAATA ACATCAACAA GTGCGCCCAC CAGCCCATGG
    CAACCTGAAT GCAGGCATCA GACGACGACT ACTTCGCCAA CACTAGCAAA AGCTGAACAA
    GTTGGTATTT TGATTCCTGA GGCAGTGCCA GGTTCACAAG TTGTCACACC GCAGACCTTT
    GCAGATGATA TCGTAATCAC AACTTTGCCT ACAAGGCTTC AAGTGACACC TGGAATTACC
    CCAAAGCCTG ATCTTGTCAC AGCCTTTAAG GACACAGTAA AACCTATTTC TGAAACTGAA
    CCTGAGGTCA TATTGGGTAC TACTTCTGAG AGTCCTGTTA AAGCATTGGA TACCATTACC
    ATTAAACATG AAGAGGTTGT TGTAGTCTCA GAATCTAAAT TATTGATTAC TGAGAGGACT
    TCGGAGGACT CTACTTCAAG GAAATATGAT TATGAGTTTC CCACGACCTT TAAGGACTCG
    TTAGTAATAA ACACTCCTAT AGTACTAACA ACTGAATTTT CTGAACAAAT GTATTCAGAA
    ATAGGACAAG GTTTTTCTGA TGGTACAGTT AAAACAGTAG CTGTTACTAT TCAAAAGGAA
    CTTGACATTG TCTCAAAATC TACATTATTA ACCACTGACA AAACTCCAGA AGTTTCTACT
    TCAAAAAAAT CTGAGCATGA TAATCCCACT AACTTTGTAG ATTCATTGGG AATAAGCACC
    AATACTCCTA GAGTGACCAC AAGTGAACTC AAAGAAGATC TTCATTCAGA ATTAGAACAG
    GTGGCTGGGC AGGATGTTAT TCACAGTCCT GTTAAAACAA CACATGTTAC AATTAAGCAT
    GAAGTGTTGG ACACAAATAC TACATTAATG ACAACTGACA GAACTCCATA TTCTACTTCA
    GCAAAATATG AACGTGTTAT ACCAACATCC TTTGAGGACT CACTAGTCAC AAGTTCATCT
    ACTGAACAGG ATATGACCAA TGTTCCTATT AAGACGGCAT ATGCCACAAT TTCACATGGA
    GAGTCTGAAA TTGTCACAGA GTCGACAATA ACAACCACTA ACAGAAATCT TGAAGATTCT
    TCTTTAGCAA AATATGATTA TGGGATTCCT CAAGGAATTG ATACTTCTTC GGGAGTAATT
    AATGAATCTT CTACAAAAGA ACATAATTCA GTATCTAAAT TGAATATTGG TCAGACTACT
    ACTGAAGGTC CCCAAACAAC TGATGCAGTT CAGTATGTAG AATCTGACAT TGTGTCAGAG
    GAAACGTTGA AATCCACTGG CAGAACTGCA GTTAGCTCAA ACTCTACAAA AATTGAGGCC
    GATCTTTCCA CAACTCCTGA GGACCTGACA GGAGTAAGTA CCAACACTCC TGAAATAGTC
    ACTAGAGATT CCATTACAAT ACTTATCCCA GAAACCGAGG GCATTACTAA AAATCCTGTT
    AAATCTGCAC CTATTACCAT TCAGCATGTG GATGTTGATG ATGTTTTAGC AGCTACAGTG
    ATGTCCACTC ATAAACCTCC CAAAGTCTCA CCTACAGAAA TATCCCCCGA TCAGAATCTC
    TTACCAGAGG AATCAAAGCT GCCAACTCCT ACAAAACTAG CAGAAGTTTT GTCTGCCACA
    GAAGAGTTGC CAAAGGATCA GACAGACAAG GATGTTGCTA AAATTGTATA TGTGTCTACT
    GTGCCAGAAC TGGTGTCACC AACTATTCCC GCATATACGT CAACTGACAC TACTAAGGAA
    AACCTACATG TGTTAACAGG GGGGGCAGTT GTTACATCAC TTGATAAAGA TGAGGAAGAA
    CATACACCCC ACACAAAAGA GAGCAAAATT ACACAGGTGA CAACAGGAGC AGAATTACCA
    GGTACCATAA CAGAGAGTAC TCATGGAATG TGGAGGGAAA CCCAAGAGCC AATAAATGTC
    ACACCTGATG CTGAAGACAA TCATTTGGGC AAAGAAGGGT ACATAATAAC AGAGATCCCA
    GAAGTTGCTG AAGTACCAGC TGGAGTGACA CCCAAAATGA TTGTGGAAAC TACAACAGCC
    ACTGAATCTG TGTTTACTGT GAAAGACACA TCTATAATTG TAGACAAAAC TGGCATTGTG
    AAACCTGTCG TGACTACGAC AGAGGCAGCT GAACTTACTG AGGTTGGATT CGAAGATTTA
    GGCACAGACA AAGTAACAAT TCTTGAAAAC ACACTTGAAG ATTCTGTAGG ACTAACAAAT
    GAACCTTTTG CTGTATCATG GGGTGTAACT GAAGCCACTA TTTTAGAGGA ATCATATGTG
    GAAAGTACTG AAAGTATTTC AGGTAAAGAG AAGTCCACTG AAACTATTAA GATCACAGGT
    GAAGCCACAA CTGATGACAT TGGTACTTCT GAAACTGACA TTGTAGTGCC TGTAACCGAT
    CGCATAGAAG ATAATGTAAA TGTATCACCT AAAAATGATG ATGCTAAGGA AACAAACATT
    TTTGAAGACA CAACACCAGA TCTTAGTGTT ACTAATTCTG AAAAAGACAT ACTGACAACT
    ACCATACATA AAGACATGCA TGAAGTTACA GAACCATTAA ATATTCCAGA GGTCACACAT
    AAGGTTGTGA TAGAGATCAC AGATGATGTA CGTGAGACCA GAGGAGATTC CTTACCCGAG
    ACACCAGAAG TCACAACAGA GACACCTAAG GTTGCAACAG AAGCTGATGT CAAAACAACA
    ACAAATGTTA TATCTAAAGC ACCTGAAGTT ATACTGAAGA CAACTGTAAC CAAGTCAGAT
    ACACCTGATG TCACAACTCA GACACCTTTG TTAAAGCTTG AAGATACAGA GAGGGTTGAA
    ATAGTACCTG AAACTCCTGA CATACAAGTG GTTCAAACAG AACACTCAGA AAATGATATC
    CCAACTCCTG TCTTGAAAGT GGAGGATTTC ACAGTTGCGC TGAATGACTT GACAACTCCT
    ATTCAAAAGA CTTCTTCAAC CACATTACAA CAAGACATTT CTAATGATAT TTTAGAAGAA
    AACAATGTGA TGGGAAATGA AATTGATGAT ATTGTGGTTC GACCAGTACG CCCTGTCGGC
    GATCATATTT TGGAGCTAAG TATTAAGCTA AGAGGGGAAA GCTATGACGA TGCTCTGAGG
    GATCCATCAA GTAACTACTA TCAACATCTG TCTACACTTT TTGTTGAAAA GATTGAAGAA
    GTTTTCGAAA GGCTTCCAGG TTTCAAGAAA ATATATGTTC TTGAATTCAG GCCACAGAAG
    GACATTGAAG GAGGTCTGGC AGTGGTGGTG CACTATGCTG TTGTTCTTGA GGGTGATGGG
    TCAGGAGTAA GTAATGAGAC CATGGATTAC ATAAACCTGC ATTCCAACAT GGTTGAGAAC
    TCCTTCACAG ATCCTGAGGA GCTGCCCACT GTGGTCTACA CCATCACTGA CTTCCGAAAC
    TACATCTCCG AAGCCCTTCA CAAGGATAAC TTTATCAGAA ACGCTACTCT GAATGTGGAC
    ATTGATTCTT TGCAACTTGA AAATGTGGAG ACATTGCTGC CTTCCAAACC AACCAGCAGA
    CCGTTTGACT CCAGTGACAT GATGGACAAC GTTCTTGCTT CCGAGAAACC ACCAGATGTT
    CCTGGGCAAG AATTATCCAA CAATGACATC TTCATTACAA ATTATGACAT CTTTGATCCT
    GATCCATGGA CAGGTGGACA AAGTGAAGAC AGAAATGCAA ATGATGTAAT CATCTTTGAG
    GAAAGTCCCA CTCAGTCTCC TGTTGAAGTG TCTTTGAAGA ACTTGGACAT TGAAGTTACT
    TCAAAATCTG AAACTTCAAG CAGTGCCCCT GTTCCTGATG AAGCTGTTAT TGAAGAGGAG
    GGATTTTTAC AGACTGCAAC TACAGTCAGT CCTATTATAG ACACAATAAC TGAATTTACT
    TTACCTACAG AATCACCTCA CAAGGTAGAA GAGACACCAG TTTCTCCTGT AGAACCTCCT
    GAGGTTGAGG AAACTACCCT GGGTGATTTA ACAGACCTTG GCTCAGGCTC AGGATTCTCT
    GGAGATCACA TAGGCCCTGA CATCTGGCCA TGGTCATCAG AAACATCACA AGAAGTCTTG
    AAGGAAGATA CCAATGATGA GATACACCAA CTTCAGGAAA ATCAGGAATC TATAATAAAA
    CAGCTTGAGC CTTTACATCA AGATGTGACT CCTGAGGAGC CATTCTTGGA TAGGGTTCTT
    GTGACCCAAG ACATTCGTAC CAATCCTCAT TACACAACAA CTGACCAGGC CCCTGTATTT
    TGGACAATGG AGACTTTGAC TGTTGAGCTT TCAATGCAGA CTCAAGTAGC CCCTGAACAT
    TATGATGACT ATTTTCCAGA TGCATCAATC ACTATGGCTA CACTTGTAAC CAATCAACCA
    TTGTTACATG TTCACAATCC ATTAGAAACA AAAGATCTGG ATGCATCACA AAGTCCAGGA
    ACAATAAGTT CAACTGCTGA TAAGGACTAT ACAGAACGAC TTGTTACACC CACTGTGATC
    CAACCAAGTG AAACAGTAGC TGTGGATAAG CATACTACAA CAGACAACAA ACAGAATCTG
    GAAACTATGG GTACATTATC TGCAACACTG GGCTCCACAA TGAAGTCCAC TGAAATCTCT
    TCAACTACCC AATTACCTGT GATCATCAAC AATAATACAG TTTCAGTGGA AGGTCCTGCC
    AATTCTTCAG AAGCTGTTCA GCATTCTGTA AATGAAGGAA TCACAGAGTT ACCTGCTTTG
    GTCTGGCCAG ATATTGAAGG ATCAGATGAA GAATTCAAAG TTTTAGATGA GGACATGGAA
    CTCGTCAAGT TTGTGCCTAC AAAAAGCCCA GTGACTGAAC TCTTAGAAGA AGATTTAGTT
    GGTGATGAAA TATTGGTTGC AACAACAACA ACAACAACAA CTCTATCCTC AACAACAGTA
    GCGACTGTGG TAACAAGTTT AGGTCATTCA GCCTCCCTGA CTCCTGAAAA GGACTCACCT
    TTCACTTGGA TATCACATTC ATCAATAGAT GAGGATGAAC CTGTGCAAGA GTCAACCACA
    GAACCTCTAC CCACCCAATC ACCCACTGCT TCTGTTCTTC CAAAGACAAC TACACAGACA
    TTGATGCACA CATACATAGC AGCTGTTGCA GGGGAGCCTG TTTTATCAGA TAACATGGAT
    AATAAGGACT CAACCACAAT GTCCACCCTT TTAGATGTTT CAAAACATTT AAAGACCCTT
    AGTTCCACCT CTGTACAATC ATTTTCAGAA CCAACATTTA CACCTACTGT CCACATAATT
    AAGCATGGAG AAACTTCAGA TGACTCAGTA CAAGATGTAG TTGAGACTGG AGAAGTTTTA
    GATGACTCTG TACAAGATGT ACTTGAGCAT GAAGACATTT CATATGGCTC TGTACAAGAT
    GTAGTTGTAT CTGGAGAAAC TTCAAATGAC TCAGTACAAG ATGTAGTTGA GACTGGAGAA
    GTTTTAGATG ATTCTGTACA AGATGTACTT GAGCATGAGG ACATTTTATC TGGCCCAGTT
    CAAGATGTAG TTGTGTCTGG AGAAACTGCA GAAGACTCAG TACAAGATTT AGTTGAGACT
    AGAGAAGTTT TAGATGATTC TGTACAAGAT GTACTTGAGC ATGAGGACAT TTTATCTGGC
    CCAGTTCAAG ATGTAGTTGT GTCTGGAGAA ACTGCAGATG ACTCAGTACA AGATTTAGTT
    GAGACTGGAG AAGTTTTAGA TGATTCCGTA CAAGATGTAC TTGAGCATGA AGTAATTTCA
    TTTGGCCCAG TACAAGATGT AGTTGTATCT GGAAAAACTT CAAATGACTC AGTACAAGAT
    GTAGTTGAGA GTGCAAAAGA TTCAGATGCC CCAATACAAG ATGTATTTGA GCATAAAGAC
    ATTTCAGTTG ACTCTGTACA AGATGTAGTT GTGTCTGGAG AAACTGCAGA TGACTCTATT
    CAAGATTTAT TTAAGCATGA AGGCACTTCG TTCGACACAG TACAAGATGA GGTTGAGTCT
    AGAGAAACTT CAGATGACTC AGTACAAAAT GTAGTTGAGC ATGAAGACTC TTCAGATGAC
    GCAGTTCAAG ATGTAGTCGA GTCTGGAGAC ACTTCAGATG ACTCTGTACA AGATATATTT
    GAGTCTGCAG AAACTTCAGA TGACTCTGTA CAAGATGTAG CTGAGACTGG AAAAGAATCA
    GATGACTTAG TACAAGATAT ATTTGAGAAT AAAGACATTT CAGTTGACTC AGTACAAGAT
    GTAGTTGTAT CTGGAGAGAC TTCAGATGAC TCTATTCAAG GTTTACTTGA GCATGAAGGC
    ACTTCAGTCG ACATAGTACA AGATGAGGTT GAGTTTAGAG AAAATTCAGA TGACTCAGTA
    CTAAATGTAG TCAAGCATGA AGATACTTCA GATGACTCAA TACAAGATGT AGTCGAGACA
    GGAGAAGTTT CAGATAGCTC AGTACAAGAT GTAGCTGAGT CTGGAGAAAC TTTAGATGAC
    TCTGTTCAAG ATTTTGTTAA AACTGGAGAA ATTTCCATTG ACTCAGTACA CAATTTATTT
    GAGCCTGAAA AAAACGTAGA TGACTCTGTA CAAGATGTAG TTGAGACTGG AGAAGTTTCT
    GTTGATTCAG TACAAGATGT AATTGAGCTT GGAGACACTT TAGATGACTC TGCACAAGGA
    ATAGTTGAAC CTGAAGAAAT GGCAGACAAC TTTATAAAGG AAAGCACTGT AAAGCCAAGA
    CCTGACATCA AAGGGCTTAT TCTACCAACA GAGACAGTGA ACTATGAAAG TGCCACTATG
    AAAGTTCCTG CTCCAGTTAA CCCCAGCATA ACAGAATTTA AGGTTTCATT TGACATCTTC
    CCTTTCGACG ACCCAACTGA TGATGAAGAT AATGGCAGTG GGTTTGCTCA TGGAACTGAC
    TTAGCAAGTT TAGCCTTGCC TGCCAGTCCT GGAAGGGCAT TAATAGTGTT TTTTAGCCTT
    AGGGTCACCA ACATGAAGTT TTCCGAAGAT CTCTTCAACA AGAGCTCTAC TGAATACAAG
    GCACTTGAGC AGCGATTTCT GGAACTGCTC GTACCGTATC TACAGTCCAA CCTCAGCAAT
    TTCCAGAATT TAGAGATTTT GAACTTCAGG AATGGTAGCA TTGTGGTGAA CAGCAGGATG
    AAGTTTGCCA AGCCAGTTCC CCATGGTGTG ACAACAGCAG TGTACCTAAT CTTGGAGGAC
    TTCTGCAACA CTGCCTACCA AACTATGAAT CTTGCAATAG ACAAGTATTC ATTGGATGTC
    GAATCAGGTG ACCAGGCAGA TCCTTGTAAG TTTCAGGCAT GTAACGAGTT CTCCAAGTGT
    TTGGTGAACC GCTGGTCAGG TGAGGCCGAG TGTGTTTGTA ACGCTGGTTA TTTCAGCGTT
    GACGGACTGC CATGCCAGAG CATCTGCGAC ATCCAAGAAG ACTTCTGCCT AAATGACGGG
    AAATGTGACA TAATACCTGG CAAGGGCGCC ATCTGCAGGT GTCGCGTTGG TGAAAACTGG
    TGGTATCGAG GAGAACACTG TGAGGAATAT GTGTCAGAGC CGCTGGTGGT CGGCATTGCC
    ATTGCGTCCG TTGCTGGATT TCTCCTGGTG GCGTCGGGTG TCATCTTTTT CCTGGCCCGA
    ACTCTGAGAG ACCAGTATGA CACAGATGAT TCAGAAGACC CTTTACGATA TGCAGAGAAC
    TTGCCATCTC TAGAAAGAGC CACCAAGTAC AACCCGATGT TTGAAAGTGA AGTGACTGCA
    GGCCATAATC AGTATTATCG GCGTTACCCA GAAGCTCCGG CATTCAGCAC GGGCAGCACC
    GAGATCTCCA CTGACTTCAG CAGCGAAGAG ATTCGTCACA TCTATCAGAA CAGTGAACTT
    ACTAAAGAGG AAATCCAGGA CCGTATTCGT ATTATAGAGC TTTACGCCAA GGACCGCCAG
    TTTGCAGATT TTGTCCGACA GCACCAAGTG GCGCTTGACG CTAGACGAGA GAGCTCTTCC
    AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009294722.1
    CDS212..8677
    Translation

    Target ORF information:

    RefSeq Version XM_009296447.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio interphotoreceptor matrix proteoglycan 2b (impg2b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296447.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGCCTGATT TACCTTTTAA ATGCATTTGG TGGGGTTTGA CACTATTGTT GTTGACTGGA 
    TACTTTTCTA TAGATGCAGA TACTGGAACT CATGAGGATT ACTTGGCAAA TGGAAATACA
    ATGCTTTTGG ACACTAATGA AGTTTTGAAT CACTTATTGT TGTTGGACAA AGGTAAAACT
    TTGAATTACA TGTCTGCTGA AGGGAAAACA GCTCTATCTA GACATAAAAG GGACCTCCTG
    TTCCCCAATG GAGTTAAACT GTGCTCACAT GAGACGGTGC AACAGGCAGT GCAGAATCAT
    CTCAACTACT TTCACCTGCG AGTGTGCCAG GAGACAATTT GGGAGGCTTA TAAAATCTTC
    TGGGATCGCT TACCTGAGAA AGAAGAGTAC CAGATCTGGA TGAAAAAATG TCAAAATGGT
    AGCATTAGCG TGTTTGACAT CGGCCGAAGC TTTAGTCAGT CAGCTGAGCA CCTTGCTTTG
    ATTTCAAGTA GGGTAGCATC ATCCACAATA ACATCAACAA GTGCGCCCAC CAGCCCATGG
    CAACCTGAAT GCAGGCATCA GACGACGACT ACTTCGCCAA CACTAGCAAA AGCTGAACAA
    GTTGGTATTT TGATTCCTGA GGCAGTGCCA GGTTCACAAG TTGTCACACC GCAGACCTTT
    GCAGATGATA TCGTAATCAC AACTTTGCCT ACAAGGCTTC AAGTGACACC TGGAATTACC
    CCAAAGCCTG ATCTTGTCAC AGCCTTTAAG GACACAGTAA AACCTATTTC TGAAACTGAA
    CCTGAGGTCA TATTGGGTAC TACTTCTGAG AGTCCTGTTA AAGCATTGGA TACCATTACC
    ATTAAACATG AAGAGGTTGT TGTAGTCTCA GAATCTAAAT TATTGATTAC TGAGAGGACT
    TCGGAGGACT CTACTTCAAG GAAATATGAT TATGAGTTTC CCACGACCTT TAAGGACTCG
    TTAGTAATAA ACACTCCTAT AGTACTAACA ACTGAATTTT CTGAACAAAT GTATTCAGAA
    ATAGGACAAG GTTTTTCTGA TGGTACAGTT AAAACAGTAG CTGTTACTAT TCAAAAGGAA
    CTTGACATTG TCTCAAAATC TACATTATTA ACCACTGACA AAACTCCAGA AGTTTCTACT
    TCAAAAAAAT CTGAGCATGA TAATCCCACT AACTTTGTAG ATTCATTGGG AATAAGCACC
    AATACTCCTA GAGTGACCAC AAGTGAACTC AAAGAAGATC TTCATTCAGA ATTAGAACAG
    GTGGCTGGGC AGGATGTTAT TCACAGTCCT GTTAAAACAA CACATGTTAC AATTAAGCAT
    GAAGTGTTGG ACACAAATAC TACATTAATG ACAACTGACA GAACTCCATA TTCTACTTCA
    GCAAAATATG AACGTGTTAT ACCAACATCC TTTGAGGACT CACTAGTCAC AAGTTCATCT
    ACTGAACAGG ATATGACCAA TGTTCCTATT AAGACGGCAT ATGCCACAAT TTCACATGGA
    GAGTCTGAAA TTGTCACAGA GTCGACAATA ACAACCACTA ACAGAAATCT TGAAGATTCT
    TCTTTAGCAA AATATGATTA TGGGATTCCT CAAGGAATTG ATACTTCTTC GGGAGTAATT
    AATGAATCTT CTACAAAAGA ACATAATTCA GTATCTAAAT TGAATATTGG TCAGACTACT
    ACTGAAGGTC CCCAAACAAC TGATGCAGTT CAGTATGTAG AATCTGACAT TGTGTCAGAG
    GAAACGTTGA AATCCACTGG CAGAACTGCA GTTAGCTCAA ACTCTACAAA AATTGAGGCC
    GATCTTTCCA CAACTCCTGA GGACCTGACA GGAGTAAGTA CCAACACTCC TGAAATAGTC
    ACTAGAGATT CCATTACAAT ACTTATCCCA GAAACCGAGG GCATTACTAA AAATCCTGTT
    AAATCTGCAC CTATTACCAT TCAGCATGTG GATGTTGATG ATGTTTTAGC AGCTACAGTG
    ATGTCCACTC ATAAACCTCC CAAAGTCTCA CCTACAGAAA TATCCCCCGA TCAGAATCTC
    TTACCAGAGG AATCAAAGCT GCCAACTCCT ACAAAACTAG CAGAAGTTTT GTCTGCCACA
    GAAGAGTTGC CAAAGGATCA GACAGACAAG GATGTTGCTA AAATTGTATA TGTGTCTACT
    GTGCCAGAAC TGGTGTCACC AACTATTCCC GCATATACGT CAACTGACAC TACTAAGGAA
    AACCTACATG TGTTAACAGG GGGGGCAGTT GTTACATCAC TTGATAAAGA TGAGGAAGAA
    CATACACCCC ACACAAAAGA GAGCAAAATT ACACAGGTGA CAACAGGAGC AGAATTACCA
    GGTACCATAA CAGAGAGTAC TCATGGAATG TGGAGGGAAA CCCAAGAGCC AATAAATGTC
    ACACCTGATG CTGAAGACAA TCATTTGGGC AAAGAAGGGT ACATAATAAC AGAGATCCCA
    GAAGTTGCTG AAGTACCAGC TGGAGTGACA CCCAAAATGA TTGTGGAAAC TACAACAGCC
    ACTGAATCTG TGTTTACTGT GAAAGACACA TCTATAATTG TAGACAAAAC TGGCATTGTG
    AAACCTGTCG TGACTACGAC AGAGGCAGCT GAACTTACTG AGGTTGGATT CGAAGATTTA
    GGCACAGACA AAGTAACAAT TCTTGAAAAC ACACTTGAAG ATTCTGTAGG ACTAACAAAT
    GAACCTTTTG CTGTATCATG GGGTGTAACT GAAGCCACTA TTTTAGAGGA ATCATATGTG
    GAAAGTACTG AAAGTATTTC AGGTAAAGAG AAGTCCACTG AAACTATTAA GATCACAGGT
    GAAGCCACAA CTGATGACAT TGGTACTTCT GAAACTGACA TTGTAGTGCC TGTAACCGAT
    CGCATAGAAG ATAATGTAAA TGTATCACCT AAAAATGATG ATGCTAAGGA AACAAACATT
    TTTGAAGACA CAACACCAGA TCTTAGTGTT ACTAATTCTG AAAAAGACAT ACTGACAACT
    ACCATACATA AAGACATGCA TGAAGTTACA GAACCATTAA ATATTCCAGA GGTCACACAT
    AAGGTTGTGA TAGAGATCAC AGATGATGTA CGTGAGACCA GAGGAGATTC CTTACCCGAG
    ACACCAGAAG TCACAACAGA GACACCTAAG GTTGCAACAG AAGCTGATGT CAAAACAACA
    ACAAATGTTA TATCTAAAGC ACCTGAAGTT ATACTGAAGA CAACTGTAAC CAAGTCAGAT
    ACACCTGATG TCACAACTCA GACACCTTTG TTAAAGCTTG AAGATACAGA GAGGGTTGAA
    ATAGTACCTG AAACTCCTGA CATACAAGTG GTTCAAACAG AACACTCAGA AAATGATATC
    CCAACTCCTG TCTTGAAAGT GGAGGATTTC ACAGTTGCGC TGAATGACTT GACAACTCCT
    ATTCAAAAGA CTTCTTCAAC CACATTACAA CAAGACATTT CTAATGATAT TTTAGAAGAA
    AACAATGTGA TGGGAAATGA AATTGATGAT ATTGTGGTTC GACCAGTACG CCCTGTCGGC
    GATCATATTT TGGAGCTAAG TATTAAGCTA AGAGGGGAAA GCTATGACGA TGCTCTGAGG
    GATCCATCAA GTAACTACTA TCAACATCTG TCTACACTTT TTGTTGAAAA GATTGAAGAA
    GTTTTCGAAA GGCTTCCAGG TTTCAAGAAA ATATATGTTC TTGAATTCAG GCCACAGAAG
    GACATTGAAG GAGGTCTGGC AGTGGTGGTG CACTATGCTG TTGTTCTTGA GGGTGATGGG
    TCAGGAGTAA GTAATGAGAC CATGGATTAC ATAAACCTGC ATTCCAACAT GGTTGAGAAC
    TCCTTCACAG ATCCTGAGGA GCTGCCCACT GTGGTCTACA CCATCACTGA CTTCCGAAAC
    TACATCTCCG AAGCCCTTCA CAAGGATAAC TTTATCAGAA ACGCTACTCT GAATGTGGAC
    ATTGATTCTT TGCAACTTGA AAATGTGGAG ACATTGCTGC CTTCCAAACC AACCAGCAGA
    CCGTTTGACT CCAGTGACAT GATGGACAAC GTTCTTGCTT CCGAGAAACC ACCAGATGTT
    CCTGGGCAAG AATTATCCAA CAATGACATC TTCATTACAA ATTATGACAT CTTTGATCCT
    GATCCATGGA CAGGTGGACA AAGTGAAGAC AGAAATGCAA ATGATGTAAT CATCTTTGAG
    GAAAGTCCCA CTCAGTCTCC TGTTGAAGTG TCTTTGAAGA ACTTGGACAT TGAAGTTACT
    TCAAAATCTG AAACTTCAAG CAGTGCCCCT GTTCCTGATG AAGCTGTTAT TGAAGAGGAG
    GGATTTTTAC AGACTGCAAC TACAGTCAGT CCTATTATAG ACACAATAAC TGAATTTACT
    TTACCTACAG AATCACCTCA CAAGGTAGAA GAGACACCAG TTTCTCCTGT AGAACCTCCT
    GAGGTTGAGG AAACTACCCT GGGTGATTTA ACAGACCTTG GCTCAGGCTC AGGATTCTCT
    GGAGATCACA TAGGCCCTGA CATCTGGCCA TGGTCATCAG AAACATCACA AGAAGTCTTG
    AAGGAAGATA CCAATGATGA GATACACCAA CTTCAGGAAA ATCAGGAATC TATAATAAAA
    CAGCTTGAGC CTTTACATCA AGATGTGACT CCTGAGGAGC CATTCTTGGA TAGGGTTCTT
    GTGACCCAAG ACATTCGTAC CAATCCTCAT TACACAACAA CTGACCAGGC CCCTGTATTT
    TGGACAATGG AGACTTTGAC TGTTGAGCTT TCAATGCAGA CTCAAGTAGC CCCTGAACAT
    TATGATGACT ATTTTCCAGA TGCATCAATC ACTATGGCTA CACTTGTAAC CAATCAACCA
    TTGTTACATG TTCACAATCC ATTAGAAACA AAAGATCTGG ATGCATCACA AAGTCCAGGA
    ACAATAAGTT CAACTGCTGA TAAGGACTAT ACAGAACGAC TTGTTACACC CACTGTGATC
    CAACCAAGTG AAACAGTAGC TGTGGATAAG CATACTACAA CAGACAACAA ACAGAATCTG
    GAAACTATGG GTACATTATC TGCAACACTG GGCTCCACAA TGAAGTCCAC TGAAATCTCT
    TCAACTACCC AATTACCTGT GATCATCAAC AATAATACAG TTTCAGTGGA AGGTCCTGCC
    AATTCTTCAG AAGCTGTTCA GCATTCTGTA AATGAAGGAA TCACAGAGTT ACCTGCTTTG
    GTCTGGCCAG ATATTGAAGG ATCAGATGAA GAATTCAAAG TTTTAGATGA GGACATGGAA
    CTCGTCAAGT TTGTGCCTAC AAAAAGCCCA GTGACTGAAC TCTTAGAAGA AGATTTAGTT
    GGTGATGAAA TATTGGTTGC AACAACAACA ACAACAACAA CTCTATCCTC AACAACAGTA
    GCGACTGTGG TAACAAGTTT AGGTCATTCA GCCTCCCTGA CTCCTGAAAA GGACTCACCT
    TTCACTTGGA TATCACATTC ATCAATAGAT GAGGATGAAC CTGTGCAAGA GTCAACCACA
    GAACCTCTAC CCACCCAATC ACCCACTGCT TCTGTTCTTC CAAAGACAAC TACACAGACA
    TTGATGCACA CATACATAGC AGCTGTTGCA GGGGAGCCTG TTTTATCAGA TAACATGGAT
    AATAAGGACT CAACCACAAT GTCCACCCTT TTAGATGTTT CAAAACATTT AAAGACCCTT
    AGTTCCACCT CTGTACAATC ATTTTCAGAA CCAACATTTA CACCTACTGT CCACATAATT
    AAGCATGGAG AAACTTCAGA TGACTCAGTA CAAGATGTAG TTGAGACTGG AGAAGTTTTA
    GATGACTCTG TACAAGATGT ACTTGAGCAT GAAGACATTT CATATGGCTC TGTACAAGAT
    GTAGTTGTAT CTGGAGAAAC TTCAAATGAC TCAGTACAAG ATGTAGTTGA GACTGGAGAA
    GTTTTAGATG ATTCTGTACA AGATGTACTT GAGCATGAGG ACATTTTATC TGGCCCAGTT
    CAAGATGTAG TTGTGTCTGG AGAAACTGCA GAAGACTCAG TACAAGATTT AGTTGAGACT
    AGAGAAGTTT TAGATGATTC TGTACAAGAT GTACTTGAGC ATGAGGACAT TTTATCTGGC
    CCAGTTCAAG ATGTAGTTGT GTCTGGAGAA ACTGCAGATG ACTCAGTACA AGATTTAGTT
    GAGACTGGAG AAGTTTTAGA TGATTCCGTA CAAGATGTAC TTGAGCATGA AGTAATTTCA
    TTTGGCCCAG TACAAGATGT AGTTGTATCT GGAAAAACTT CAAATGACTC AGTACAAGAT
    GTAGTTGAGA GTGCAAAAGA TTCAGATGCC CCAATACAAG ATGTATTTGA GCATAAAGAC
    ATTTCAGTTG ACTCTGTACA AGATGTAGTT GTGTCTGGAG AAACTGCAGA TGACTCTATT
    CAAGATTTAT TTAAGCATGA AGGCACTTCG TTCGACACAG TACAAGATGA GGTTGAGTCT
    AGAGAAACTT CAGATGACTC AGTACAAAAT GTAGTTGAGC ATGAAGACTC TTCAGATGAC
    GCAGTTCAAG ATGTAGTCGA GTCTGGAGAC ACTTCAGATG ACTCTGTACA AGATATATTT
    GAGTCTGCAG AAACTTCAGA TGACTCTGTA CAAGATGTAG CTGAGACTGG AAAAGAATCA
    GATGACTTAG TACAAGATAT ATTTGAGAAT AAAGACATTT CAGTTGACTC AGTACAAGAT
    GTAGTTGTAT CTGGAGAGAC TTCAGATGAC TCTATTCAAG GTTTACTTGA GCATGAAGGC
    ACTTCAGTCG ACATAGTACA AGATGAGGTT GAGTTTAGAG AAAATTCAGA TGACTCAGTA
    CTAAATGTAG TCAAGCATGA AGATACTTCA GATGACTCAA TACAAGATGT AGTCGAGACA
    GGAGAAGTTT CAGATAGCTC AGTACAAGAT GTAGCTGAGT CTGGAGAAAC TTTAGATGAC
    TCTGTTCAAG ATTTTGTTAA AACTGGAGAA ATTTCCATTG ACTCAGTACA CAATTTATTT
    GAGCCTGAAA AAAACGTAGA TGACTCTGTA CAAGATGTAG TTGAGACTGG AGAAGTTTCT
    GTTGATTCAG TACAAGATGT AATTGAGCTT GGAGACACTT TAGATGACTC TGCACAAGGA
    ATAGTTGAAC CTGAAGAAAT GGCAGACAAC TTTATAAAGG AAAGCACTGT AAAGCCAAGA
    CCTGACATCA AAGGGCTTAT TCTACCAACA GAGACAGTGA ACTATGAAAG TGCCACTATG
    AAAGTTCCTG CTCCAGTTAA CCCCAGCATA ACAGAATTTA AGGTTTCATT TGACATCTTC
    CCTTTCGACG ACCCAACTGA TGATGAAGAT AATGGCAGTG GGTTTGCTCA TGGAACTGAC
    TTAGCAAGTT TAGCCTTGCC TGCCAGTCCT GGAAGGGCAT TAATAGTGTT TTTTAGCCTT
    AGGGTCACCA ACATGAAGTT TTCCGAAGAT CTCTTCAACA AGAGCTCTAC TGAATACAAG
    GCACTTGAGC AGCGATTTCT GGAACTGCTC GTACCGTATC TACAGTCCAA CCTCAGCAAT
    TTCCAGAATT TAGAGATTTT GAACTTCAGG AATGGTAGCA TTGTGGTGAA CAGCAGGATG
    AAGTTTGCCA AGCCAGTTCC CCATGGTGTG ACAACAGCAG TGTACCTAAT CTTGGAGGAC
    TTCTGCAACA CTGCCTACCA AACTATGAAT CTTGCAATAG ACAAGTATTC ATTGGATGTC
    GAATCAGGTG ACCAGGCAGA TCCTTGTAAG TTTCAGGCAT GTAACGAGTT CTCCAAGTGT
    TTGGTGAACC GCTGGTCAGG TGAGGCCGAG TGTGTTTGTA ACGCTGGTTA TTTCAGCGTT
    GACGGACTGC CATGCCAGAG CATCTGCGAC ATCCAAGAAG ACTTCTGCCT AAATGACGGG
    AAATGTGACA TAATACCTGG CAAGGGCGCC ATCTGCAGGT GTCGCGTTGG TGAAAACTGG
    TGGTATCGAG GAGAACACTG TGAGGAATAT GTGTCAGAGC CGCTGGTGGT CGGCATTGCC
    ATTGCGTCCG TTGCTGGATT TCTCCTGGTG GCGTCGGGTG TCATCTTTTT CCTGGCCCGA
    ACTCTGAGAG ACCAGTATGA CACAGATGAT TCAGAAGACC CTTTACGATA TGCAGAGAAC
    TTGCCATCTC TAGAAAGAGC CACCAAGTAC AACCCGATGT TTGAAAGTGA AGTGACTGCA
    GGCCATAATC AGTATTATCG GCGTTACCCA GAAGCTCCGG CATTCAGCAC GGGCAGCACC
    GAGATCTCCA CTGACTTCAG CAGCGAAGAG ATTCGTCACA TCTATCAGAA CAGTGAACTT
    ACTAAAGAGG AAATCCAGGA CCGTATTCGT ATTATAGAGC TTTACGCCAA GGACCGCCAG
    TTTGCAGATT TTGTCCGACA GCACCAAGTG GCGCTTGACG CTAGACGAGA GAGCTCTTCC
    AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27099
    Clone ID Related Accession (Same CDS sequence) XM_021474484.1 , XM_009296447.3 , XM_009296447.2 , XM_021473520.1
    Accession Version XM_021473520.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8466bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395115.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGCCTGATT TACCTTTTAA ATGCATTTGG TGGGGTTTGA CACTATTGTT GTTGACTGGA 
    TACTTTTCTA TAGATGCAGA TACTGGAACT CATGAGGATT ACTTGGCAAA TGGAAATACA
    ATGCTTTTGG ACACTAATGA AGTTTTGAAT CACTTATTGT TGTTGGACAA AGGTAAAACT
    TTGAATTACA TGTCTGCTGA AGGGAAAACA GCTCTATCTA GACATAAAAG GGACCTCCTG
    TTCCCCAATG GAGTTAAACT GTGCTCACAT GAGACGGTGC AACAGGCAGT GCAGAATCAT
    CTCAACTACT TTCACCTGCG AGTGTGCCAG GAGACAATTT GGGAGGCTTA TAAAATCTTC
    TGGGATCGCT TACCTGAGAA AGAAGAGTAC CAGATCTGGA TGAAAAAATG TCAAAATGGT
    AGCATTAGCG TGTTTGACAT CGGCCGAAGC TTTAGTCAGT CAGCTGAGCA CCTTGCTTTG
    ATTTCAAGTA GGGTAGCATC ATCCACAATA ACATCAACAA GTGCGCCCAC CAGCCCATGG
    CAACCTGAAT GCAGGCATCA GACGACGACT ACTTCGCCAA CACTAGCAAA AGCTGAACAA
    GTTGGTATTT TGATTCCTGA GGCAGTGCCA GGTTCACAAG TTGTCACACC GCAGACCTTT
    GCAGATGATA TCGTAATCAC AACTTTGCCT ACAAGGCTTC AAGTGACACC TGGAATTACC
    CCAAAGCCTG ATCTTGTCAC AGCCTTTAAG GACACAGTAA AACCTATTTC TGAAACTGAA
    CCTGAGGTCA TATTGGGTAC TACTTCTGAG AGTCCTGTTA AAGCATTGGA TACCATTACC
    ATTAAACATG AAGAGGTTGT TGTAGTCTCA GAATCTAAAT TATTGATTAC TGAGAGGACT
    TCGGAGGACT CTACTTCAAG GAAATATGAT TATGAGTTTC CCACGACCTT TAAGGACTCG
    TTAGTAATAA ACACTCCTAT AGTACTAACA ACTGAATTTT CTGAACAAAT GTATTCAGAA
    ATAGGACAAG GTTTTTCTGA TGGTACAGTT AAAACAGTAG CTGTTACTAT TCAAAAGGAA
    CTTGACATTG TCTCAAAATC TACATTATTA ACCACTGACA AAACTCCAGA AGTTTCTACT
    TCAAAAAAAT CTGAGCATGA TAATCCCACT AACTTTGTAG ATTCATTGGG AATAAGCACC
    AATACTCCTA GAGTGACCAC AAGTGAACTC AAAGAAGATC TTCATTCAGA ATTAGAACAG
    GTGGCTGGGC AGGATGTTAT TCACAGTCCT GTTAAAACAA CACATGTTAC AATTAAGCAT
    GAAGTGTTGG ACACAAATAC TACATTAATG ACAACTGACA GAACTCCATA TTCTACTTCA
    GCAAAATATG AACGTGTTAT ACCAACATCC TTTGAGGACT CACTAGTCAC AAGTTCATCT
    ACTGAACAGG ATATGACCAA TGTTCCTATT AAGACGGCAT ATGCCACAAT TTCACATGGA
    GAGTCTGAAA TTGTCACAGA GTCGACAATA ACAACCACTA ACAGAAATCT TGAAGATTCT
    TCTTTAGCAA AATATGATTA TGGGATTCCT CAAGGAATTG ATACTTCTTC GGGAGTAATT
    AATGAATCTT CTACAAAAGA ACATAATTCA GTATCTAAAT TGAATATTGG TCAGACTACT
    ACTGAAGGTC CCCAAACAAC TGATGCAGTT CAGTATGTAG AATCTGACAT TGTGTCAGAG
    GAAACGTTGA AATCCACTGG CAGAACTGCA GTTAGCTCAA ACTCTACAAA AATTGAGGCC
    GATCTTTCCA CAACTCCTGA GGACCTGACA GGAGTAAGTA CCAACACTCC TGAAATAGTC
    ACTAGAGATT CCATTACAAT ACTTATCCCA GAAACCGAGG GCATTACTAA AAATCCTGTT
    AAATCTGCAC CTATTACCAT TCAGCATGTG GATGTTGATG ATGTTTTAGC AGCTACAGTG
    ATGTCCACTC ATAAACCTCC CAAAGTCTCA CCTACAGAAA TATCCCCCGA TCAGAATCTC
    TTACCAGAGG AATCAAAGCT GCCAACTCCT ACAAAACTAG CAGAAGTTTT GTCTGCCACA
    GAAGAGTTGC CAAAGGATCA GACAGACAAG GATGTTGCTA AAATTGTATA TGTGTCTACT
    GTGCCAGAAC TGGTGTCACC AACTATTCCC GCATATACGT CAACTGACAC TACTAAGGAA
    AACCTACATG TGTTAACAGG GGGGGCAGTT GTTACATCAC TTGATAAAGA TGAGGAAGAA
    CATACACCCC ACACAAAAGA GAGCAAAATT ACACAGGTGA CAACAGGAGC AGAATTACCA
    GGTACCATAA CAGAGAGTAC TCATGGAATG TGGAGGGAAA CCCAAGAGCC AATAAATGTC
    ACACCTGATG CTGAAGACAA TCATTTGGGC AAAGAAGGGT ACATAATAAC AGAGATCCCA
    GAAGTTGCTG AAGTACCAGC TGGAGTGACA CCCAAAATGA TTGTGGAAAC TACAACAGCC
    ACTGAATCTG TGTTTACTGT GAAAGACACA TCTATAATTG TAGACAAAAC TGGCATTGTG
    AAACCTGTCG TGACTACGAC AGAGGCAGCT GAACTTACTG AGGTTGGATT CGAAGATTTA
    GGCACAGACA AAGTAACAAT TCTTGAAAAC ACACTTGAAG ATTCTGTAGG ACTAACAAAT
    GAACCTTTTG CTGTATCATG GGGTGTAACT GAAGCCACTA TTTTAGAGGA ATCATATGTG
    GAAAGTACTG AAAGTATTTC AGGTAAAGAG AAGTCCACTG AAACTATTAA GATCACAGGT
    GAAGCCACAA CTGATGACAT TGGTACTTCT GAAACTGACA TTGTAGTGCC TGTAACCGAT
    CGCATAGAAG ATAATGTAAA TGTATCACCT AAAAATGATG ATGCTAAGGA AACAAACATT
    TTTGAAGACA CAACACCAGA TCTTAGTGTT ACTAATTCTG AAAAAGACAT ACTGACAACT
    ACCATACATA AAGACATGCA TGAAGTTACA GAACCATTAA ATATTCCAGA GGTCACACAT
    AAGGTTGTGA TAGAGATCAC AGATGATGTA CGTGAGACCA GAGGAGATTC CTTACCCGAG
    ACACCAGAAG TCACAACAGA GACACCTAAG GTTGCAACAG AAGCTGATGT CAAAACAACA
    ACAAATGTTA TATCTAAAGC ACCTGAAGTT ATACTGAAGA CAACTGTAAC CAAGTCAGAT
    ACACCTGATG TCACAACTCA GACACCTTTG TTAAAGCTTG AAGATACAGA GAGGGTTGAA
    ATAGTACCTG AAACTCCTGA CATACAAGTG GTTCAAACAG AACACTCAGA AAATGATATC
    CCAACTCCTG TCTTGAAAGT GGAGGATTTC ACAGTTGCGC TGAATGACTT GACAACTCCT
    ATTCAAAAGA CTTCTTCAAC CACATTACAA CAAGACATTT CTAATGATAT TTTAGAAGAA
    AACAATGTGA TGGGAAATGA AATTGATGAT ATTGTGGTTC GACCAGTACG CCCTGTCGGC
    GATCATATTT TGGAGCTAAG TATTAAGCTA AGAGGGGAAA GCTATGACGA TGCTCTGAGG
    GATCCATCAA GTAACTACTA TCAACATCTG TCTACACTTT TTGTTGAAAA GATTGAAGAA
    GTTTTCGAAA GGCTTCCAGG TTTCAAGAAA ATATATGTTC TTGAATTCAG GCCACAGAAG
    GACATTGAAG GAGGTCTGGC AGTGGTGGTG CACTATGCTG TTGTTCTTGA GGGTGATGGG
    TCAGGAGTAA GTAATGAGAC CATGGATTAC ATAAACCTGC ATTCCAACAT GGTTGAGAAC
    TCCTTCACAG ATCCTGAGGA GCTGCCCACT GTGGTCTACA CCATCACTGA CTTCCGAAAC
    TACATCTCCG AAGCCCTTCA CAAGGATAAC TTTATCAGAA ACGCTACTCT GAATGTGGAC
    ATTGATTCTT TGCAACTTGA AAATGTGGAG ACATTGCTGC CTTCCAAACC AACCAGCAGA
    CCGTTTGACT CCAGTGACAT GATGGACAAC GTTCTTGCTT CCGAGAAACC ACCAGATGTT
    CCTGGGCAAG AATTATCCAA CAATGACATC TTCATTACAA ATTATGACAT CTTTGATCCT
    GATCCATGGA CAGGTGGACA AAGTGAAGAC AGAAATGCAA ATGATGTAAT CATCTTTGAG
    GAAAGTCCCA CTCAGTCTCC TGTTGAAGTG TCTTTGAAGA ACTTGGACAT TGAAGTTACT
    TCAAAATCTG AAACTTCAAG CAGTGCCCCT GTTCCTGATG AAGCTGTTAT TGAAGAGGAG
    GGATTTTTAC AGACTGCAAC TACAGTCAGT CCTATTATAG ACACAATAAC TGAATTTACT
    TTACCTACAG AATCACCTCA CAAGGTAGAA GAGACACCAG TTTCTCCTGT AGAACCTCCT
    GAGGTTGAGG AAACTACCCT GGGTGATTTA ACAGACCTTG GCTCAGGCTC AGGATTCTCT
    GGAGATCACA TAGGCCCTGA CATCTGGCCA TGGTCATCAG AAACATCACA AGAAGTCTTG
    AAGGAAGATA CCAATGATGA GATACACCAA CTTCAGGAAA ATCAGGAATC TATAATAAAA
    CAGCTTGAGC CTTTACATCA AGATGTGACT CCTGAGGAGC CATTCTTGGA TAGGGTTCTT
    GTGACCCAAG ACATTCGTAC CAATCCTCAT TACACAACAA CTGACCAGGC CCCTGTATTT
    TGGACAATGG AGACTTTGAC TGTTGAGCTT TCAATGCAGA CTCAAGTAGC CCCTGAACAT
    TATGATGACT ATTTTCCAGA TGCATCAATC ACTATGGCTA CACTTGTAAC CAATCAACCA
    TTGTTACATG TTCACAATCC ATTAGAAACA AAAGATCTGG ATGCATCACA AAGTCCAGGA
    ACAATAAGTT CAACTGCTGA TAAGGACTAT ACAGAACGAC TTGTTACACC CACTGTGATC
    CAACCAAGTG AAACAGTAGC TGTGGATAAG CATACTACAA CAGACAACAA ACAGAATCTG
    GAAACTATGG GTACATTATC TGCAACACTG GGCTCCACAA TGAAGTCCAC TGAAATCTCT
    TCAACTACCC AATTACCTGT GATCATCAAC AATAATACAG TTTCAGTGGA AGGTCCTGCC
    AATTCTTCAG AAGCTGTTCA GCATTCTGTA AATGAAGGAA TCACAGAGTT ACCTGCTTTG
    GTCTGGCCAG ATATTGAAGG ATCAGATGAA GAATTCAAAG TTTTAGATGA GGACATGGAA
    CTCGTCAAGT TTGTGCCTAC AAAAAGCCCA GTGACTGAAC TCTTAGAAGA AGATTTAGTT
    GGTGATGAAA TATTGGTTGC AACAACAACA ACAACAACAA CTCTATCCTC AACAACAGTA
    GCGACTGTGG TAACAAGTTT AGGTCATTCA GCCTCCCTGA CTCCTGAAAA GGACTCACCT
    TTCACTTGGA TATCACATTC ATCAATAGAT GAGGATGAAC CTGTGCAAGA GTCAACCACA
    GAACCTCTAC CCACCCAATC ACCCACTGCT TCTGTTCTTC CAAAGACAAC TACACAGACA
    TTGATGCACA CATACATAGC AGCTGTTGCA GGGGAGCCTG TTTTATCAGA TAACATGGAT
    AATAAGGACT CAACCACAAT GTCCACCCTT TTAGATGTTT CAAAACATTT AAAGACCCTT
    AGTTCCACCT CTGTACAATC ATTTTCAGAA CCAACATTTA CACCTACTGT CCACATAATT
    AAGCATGGAG AAACTTCAGA TGACTCAGTA CAAGATGTAG TTGAGACTGG AGAAGTTTTA
    GATGACTCTG TACAAGATGT ACTTGAGCAT GAAGACATTT CATATGGCTC TGTACAAGAT
    GTAGTTGTAT CTGGAGAAAC TTCAAATGAC TCAGTACAAG ATGTAGTTGA GACTGGAGAA
    GTTTTAGATG ATTCTGTACA AGATGTACTT GAGCATGAGG ACATTTTATC TGGCCCAGTT
    CAAGATGTAG TTGTGTCTGG AGAAACTGCA GAAGACTCAG TACAAGATTT AGTTGAGACT
    AGAGAAGTTT TAGATGATTC TGTACAAGAT GTACTTGAGC ATGAGGACAT TTTATCTGGC
    CCAGTTCAAG ATGTAGTTGT GTCTGGAGAA ACTGCAGATG ACTCAGTACA AGATTTAGTT
    GAGACTGGAG AAGTTTTAGA TGATTCCGTA CAAGATGTAC TTGAGCATGA AGTAATTTCA
    TTTGGCCCAG TACAAGATGT AGTTGTATCT GGAAAAACTT CAAATGACTC AGTACAAGAT
    GTAGTTGAGA GTGCAAAAGA TTCAGATGCC CCAATACAAG ATGTATTTGA GCATAAAGAC
    ATTTCAGTTG ACTCTGTACA AGATGTAGTT GTGTCTGGAG AAACTGCAGA TGACTCTATT
    CAAGATTTAT TTAAGCATGA AGGCACTTCG TTCGACACAG TACAAGATGA GGTTGAGTCT
    AGAGAAACTT CAGATGACTC AGTACAAAAT GTAGTTGAGC ATGAAGACTC TTCAGATGAC
    GCAGTTCAAG ATGTAGTCGA GTCTGGAGAC ACTTCAGATG ACTCTGTACA AGATATATTT
    GAGTCTGCAG AAACTTCAGA TGACTCTGTA CAAGATGTAG CTGAGACTGG AAAAGAATCA
    GATGACTTAG TACAAGATAT ATTTGAGAAT AAAGACATTT CAGTTGACTC AGTACAAGAT
    GTAGTTGTAT CTGGAGAGAC TTCAGATGAC TCTATTCAAG GTTTACTTGA GCATGAAGGC
    ACTTCAGTCG ACATAGTACA AGATGAGGTT GAGTTTAGAG AAAATTCAGA TGACTCAGTA
    CTAAATGTAG TCAAGCATGA AGATACTTCA GATGACTCAA TACAAGATGT AGTCGAGACA
    GGAGAAGTTT CAGATAGCTC AGTACAAGAT GTAGCTGAGT CTGGAGAAAC TTTAGATGAC
    TCTGTTCAAG ATTTTGTTAA AACTGGAGAA ATTTCCATTG ACTCAGTACA CAATTTATTT
    GAGCCTGAAA AAAACGTAGA TGACTCTGTA CAAGATGTAG TTGAGACTGG AGAAGTTTCT
    GTTGATTCAG TACAAGATGT AATTGAGCTT GGAGACACTT TAGATGACTC TGCACAAGGA
    ATAGTTGAAC CTGAAGAAAT GGCAGACAAC TTTATAAAGG AAAGCACTGT AAAGCCAAGA
    CCTGACATCA AAGGGCTTAT TCTACCAACA GAGACAGTGA ACTATGAAAG TGCCACTATG
    AAAGTTCCTG CTCCAGTTAA CCCCAGCATA ACAGAATTTA AGGTTTCATT TGACATCTTC
    CCTTTCGACG ACCCAACTGA TGATGAAGAT AATGGCAGTG GGTTTGCTCA TGGAACTGAC
    TTAGCAAGTT TAGCCTTGCC TGCCAGTCCT GGAAGGGCAT TAATAGTGTT TTTTAGCCTT
    AGGGTCACCA ACATGAAGTT TTCCGAAGAT CTCTTCAACA AGAGCTCTAC TGAATACAAG
    GCACTTGAGC AGCGATTTCT GGAACTGCTC GTACCGTATC TACAGTCCAA CCTCAGCAAT
    TTCCAGAATT TAGAGATTTT GAACTTCAGG AATGGTAGCA TTGTGGTGAA CAGCAGGATG
    AAGTTTGCCA AGCCAGTTCC CCATGGTGTG ACAACAGCAG TGTACCTAAT CTTGGAGGAC
    TTCTGCAACA CTGCCTACCA AACTATGAAT CTTGCAATAG ACAAGTATTC ATTGGATGTC
    GAATCAGGTG ACCAGGCAGA TCCTTGTAAG TTTCAGGCAT GTAACGAGTT CTCCAAGTGT
    TTGGTGAACC GCTGGTCAGG TGAGGCCGAG TGTGTTTGTA ACGCTGGTTA TTTCAGCGTT
    GACGGACTGC CATGCCAGAG CATCTGCGAC ATCCAAGAAG ACTTCTGCCT AAATGACGGG
    AAATGTGACA TAATACCTGG CAAGGGCGCC ATCTGCAGGT GTCGCGTTGG TGAAAACTGG
    TGGTATCGAG GAGAACACTG TGAGGAATAT GTGTCAGAGC CGCTGGTGGT CGGCATTGCC
    ATTGCGTCCG TTGCTGGATT TCTCCTGGTG GCGTCGGGTG TCATCTTTTT CCTGGCCCGA
    ACTCTGAGAG ACCAGTATGA CACAGATGAT TCAGAAGACC CTTTACGATA TGCAGAGAAC
    TTGCCATCTC TAGAAAGAGC CACCAAGTAC AACCCGATGT TTGAAAGTGA AGTGACTGCA
    GGCCATAATC AGTATTATCG GCGTTACCCA GAAGCTCCGG CATTCAGCAC GGGCAGCACC
    GAGATCTCCA CTGACTTCAG CAGCGAAGAG ATTCGTCACA TCTATCAGAA CAGTGAACTT
    ACTAAAGAGG AAATCCAGGA CCGTATTCGT ATTATAGAGC TTTACGCCAA GGACCGCCAG
    TTTGCAGATT TTGTCCGACA GCACCAAGTG GCGCTTGACG CTAGACGAGA GAGCTCTTCC
    AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329195.1
    CDS233..8698
    Translation

    Target ORF information:

    RefSeq Version XM_021473520.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio interphotoreceptor matrix proteoglycan 2b (impg2b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473520.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGCCTGATT TACCTTTTAA ATGCATTTGG TGGGGTTTGA CACTATTGTT GTTGACTGGA 
    TACTTTTCTA TAGATGCAGA TACTGGAACT CATGAGGATT ACTTGGCAAA TGGAAATACA
    ATGCTTTTGG ACACTAATGA AGTTTTGAAT CACTTATTGT TGTTGGACAA AGGTAAAACT
    TTGAATTACA TGTCTGCTGA AGGGAAAACA GCTCTATCTA GACATAAAAG GGACCTCCTG
    TTCCCCAATG GAGTTAAACT GTGCTCACAT GAGACGGTGC AACAGGCAGT GCAGAATCAT
    CTCAACTACT TTCACCTGCG AGTGTGCCAG GAGACAATTT GGGAGGCTTA TAAAATCTTC
    TGGGATCGCT TACCTGAGAA AGAAGAGTAC CAGATCTGGA TGAAAAAATG TCAAAATGGT
    AGCATTAGCG TGTTTGACAT CGGCCGAAGC TTTAGTCAGT CAGCTGAGCA CCTTGCTTTG
    ATTTCAAGTA GGGTAGCATC ATCCACAATA ACATCAACAA GTGCGCCCAC CAGCCCATGG
    CAACCTGAAT GCAGGCATCA GACGACGACT ACTTCGCCAA CACTAGCAAA AGCTGAACAA
    GTTGGTATTT TGATTCCTGA GGCAGTGCCA GGTTCACAAG TTGTCACACC GCAGACCTTT
    GCAGATGATA TCGTAATCAC AACTTTGCCT ACAAGGCTTC AAGTGACACC TGGAATTACC
    CCAAAGCCTG ATCTTGTCAC AGCCTTTAAG GACACAGTAA AACCTATTTC TGAAACTGAA
    CCTGAGGTCA TATTGGGTAC TACTTCTGAG AGTCCTGTTA AAGCATTGGA TACCATTACC
    ATTAAACATG AAGAGGTTGT TGTAGTCTCA GAATCTAAAT TATTGATTAC TGAGAGGACT
    TCGGAGGACT CTACTTCAAG GAAATATGAT TATGAGTTTC CCACGACCTT TAAGGACTCG
    TTAGTAATAA ACACTCCTAT AGTACTAACA ACTGAATTTT CTGAACAAAT GTATTCAGAA
    ATAGGACAAG GTTTTTCTGA TGGTACAGTT AAAACAGTAG CTGTTACTAT TCAAAAGGAA
    CTTGACATTG TCTCAAAATC TACATTATTA ACCACTGACA AAACTCCAGA AGTTTCTACT
    TCAAAAAAAT CTGAGCATGA TAATCCCACT AACTTTGTAG ATTCATTGGG AATAAGCACC
    AATACTCCTA GAGTGACCAC AAGTGAACTC AAAGAAGATC TTCATTCAGA ATTAGAACAG
    GTGGCTGGGC AGGATGTTAT TCACAGTCCT GTTAAAACAA CACATGTTAC AATTAAGCAT
    GAAGTGTTGG ACACAAATAC TACATTAATG ACAACTGACA GAACTCCATA TTCTACTTCA
    GCAAAATATG AACGTGTTAT ACCAACATCC TTTGAGGACT CACTAGTCAC AAGTTCATCT
    ACTGAACAGG ATATGACCAA TGTTCCTATT AAGACGGCAT ATGCCACAAT TTCACATGGA
    GAGTCTGAAA TTGTCACAGA GTCGACAATA ACAACCACTA ACAGAAATCT TGAAGATTCT
    TCTTTAGCAA AATATGATTA TGGGATTCCT CAAGGAATTG ATACTTCTTC GGGAGTAATT
    AATGAATCTT CTACAAAAGA ACATAATTCA GTATCTAAAT TGAATATTGG TCAGACTACT
    ACTGAAGGTC CCCAAACAAC TGATGCAGTT CAGTATGTAG AATCTGACAT TGTGTCAGAG
    GAAACGTTGA AATCCACTGG CAGAACTGCA GTTAGCTCAA ACTCTACAAA AATTGAGGCC
    GATCTTTCCA CAACTCCTGA GGACCTGACA GGAGTAAGTA CCAACACTCC TGAAATAGTC
    ACTAGAGATT CCATTACAAT ACTTATCCCA GAAACCGAGG GCATTACTAA AAATCCTGTT
    AAATCTGCAC CTATTACCAT TCAGCATGTG GATGTTGATG ATGTTTTAGC AGCTACAGTG
    ATGTCCACTC ATAAACCTCC CAAAGTCTCA CCTACAGAAA TATCCCCCGA TCAGAATCTC
    TTACCAGAGG AATCAAAGCT GCCAACTCCT ACAAAACTAG CAGAAGTTTT GTCTGCCACA
    GAAGAGTTGC CAAAGGATCA GACAGACAAG GATGTTGCTA AAATTGTATA TGTGTCTACT
    GTGCCAGAAC TGGTGTCACC AACTATTCCC GCATATACGT CAACTGACAC TACTAAGGAA
    AACCTACATG TGTTAACAGG GGGGGCAGTT GTTACATCAC TTGATAAAGA TGAGGAAGAA
    CATACACCCC ACACAAAAGA GAGCAAAATT ACACAGGTGA CAACAGGAGC AGAATTACCA
    GGTACCATAA CAGAGAGTAC TCATGGAATG TGGAGGGAAA CCCAAGAGCC AATAAATGTC
    ACACCTGATG CTGAAGACAA TCATTTGGGC AAAGAAGGGT ACATAATAAC AGAGATCCCA
    GAAGTTGCTG AAGTACCAGC TGGAGTGACA CCCAAAATGA TTGTGGAAAC TACAACAGCC
    ACTGAATCTG TGTTTACTGT GAAAGACACA TCTATAATTG TAGACAAAAC TGGCATTGTG
    AAACCTGTCG TGACTACGAC AGAGGCAGCT GAACTTACTG AGGTTGGATT CGAAGATTTA
    GGCACAGACA AAGTAACAAT TCTTGAAAAC ACACTTGAAG ATTCTGTAGG ACTAACAAAT
    GAACCTTTTG CTGTATCATG GGGTGTAACT GAAGCCACTA TTTTAGAGGA ATCATATGTG
    GAAAGTACTG AAAGTATTTC AGGTAAAGAG AAGTCCACTG AAACTATTAA GATCACAGGT
    GAAGCCACAA CTGATGACAT TGGTACTTCT GAAACTGACA TTGTAGTGCC TGTAACCGAT
    CGCATAGAAG ATAATGTAAA TGTATCACCT AAAAATGATG ATGCTAAGGA AACAAACATT
    TTTGAAGACA CAACACCAGA TCTTAGTGTT ACTAATTCTG AAAAAGACAT ACTGACAACT
    ACCATACATA AAGACATGCA TGAAGTTACA GAACCATTAA ATATTCCAGA GGTCACACAT
    AAGGTTGTGA TAGAGATCAC AGATGATGTA CGTGAGACCA GAGGAGATTC CTTACCCGAG
    ACACCAGAAG TCACAACAGA GACACCTAAG GTTGCAACAG AAGCTGATGT CAAAACAACA
    ACAAATGTTA TATCTAAAGC ACCTGAAGTT ATACTGAAGA CAACTGTAAC CAAGTCAGAT
    ACACCTGATG TCACAACTCA GACACCTTTG TTAAAGCTTG AAGATACAGA GAGGGTTGAA
    ATAGTACCTG AAACTCCTGA CATACAAGTG GTTCAAACAG AACACTCAGA AAATGATATC
    CCAACTCCTG TCTTGAAAGT GGAGGATTTC ACAGTTGCGC TGAATGACTT GACAACTCCT
    ATTCAAAAGA CTTCTTCAAC CACATTACAA CAAGACATTT CTAATGATAT TTTAGAAGAA
    AACAATGTGA TGGGAAATGA AATTGATGAT ATTGTGGTTC GACCAGTACG CCCTGTCGGC
    GATCATATTT TGGAGCTAAG TATTAAGCTA AGAGGGGAAA GCTATGACGA TGCTCTGAGG
    GATCCATCAA GTAACTACTA TCAACATCTG TCTACACTTT TTGTTGAAAA GATTGAAGAA
    GTTTTCGAAA GGCTTCCAGG TTTCAAGAAA ATATATGTTC TTGAATTCAG GCCACAGAAG
    GACATTGAAG GAGGTCTGGC AGTGGTGGTG CACTATGCTG TTGTTCTTGA GGGTGATGGG
    TCAGGAGTAA GTAATGAGAC CATGGATTAC ATAAACCTGC ATTCCAACAT GGTTGAGAAC
    TCCTTCACAG ATCCTGAGGA GCTGCCCACT GTGGTCTACA CCATCACTGA CTTCCGAAAC
    TACATCTCCG AAGCCCTTCA CAAGGATAAC TTTATCAGAA ACGCTACTCT GAATGTGGAC
    ATTGATTCTT TGCAACTTGA AAATGTGGAG ACATTGCTGC CTTCCAAACC AACCAGCAGA
    CCGTTTGACT CCAGTGACAT GATGGACAAC GTTCTTGCTT CCGAGAAACC ACCAGATGTT
    CCTGGGCAAG AATTATCCAA CAATGACATC TTCATTACAA ATTATGACAT CTTTGATCCT
    GATCCATGGA CAGGTGGACA AAGTGAAGAC AGAAATGCAA ATGATGTAAT CATCTTTGAG
    GAAAGTCCCA CTCAGTCTCC TGTTGAAGTG TCTTTGAAGA ACTTGGACAT TGAAGTTACT
    TCAAAATCTG AAACTTCAAG CAGTGCCCCT GTTCCTGATG AAGCTGTTAT TGAAGAGGAG
    GGATTTTTAC AGACTGCAAC TACAGTCAGT CCTATTATAG ACACAATAAC TGAATTTACT
    TTACCTACAG AATCACCTCA CAAGGTAGAA GAGACACCAG TTTCTCCTGT AGAACCTCCT
    GAGGTTGAGG AAACTACCCT GGGTGATTTA ACAGACCTTG GCTCAGGCTC AGGATTCTCT
    GGAGATCACA TAGGCCCTGA CATCTGGCCA TGGTCATCAG AAACATCACA AGAAGTCTTG
    AAGGAAGATA CCAATGATGA GATACACCAA CTTCAGGAAA ATCAGGAATC TATAATAAAA
    CAGCTTGAGC CTTTACATCA AGATGTGACT CCTGAGGAGC CATTCTTGGA TAGGGTTCTT
    GTGACCCAAG ACATTCGTAC CAATCCTCAT TACACAACAA CTGACCAGGC CCCTGTATTT
    TGGACAATGG AGACTTTGAC TGTTGAGCTT TCAATGCAGA CTCAAGTAGC CCCTGAACAT
    TATGATGACT ATTTTCCAGA TGCATCAATC ACTATGGCTA CACTTGTAAC CAATCAACCA
    TTGTTACATG TTCACAATCC ATTAGAAACA AAAGATCTGG ATGCATCACA AAGTCCAGGA
    ACAATAAGTT CAACTGCTGA TAAGGACTAT ACAGAACGAC TTGTTACACC CACTGTGATC
    CAACCAAGTG AAACAGTAGC TGTGGATAAG CATACTACAA CAGACAACAA ACAGAATCTG
    GAAACTATGG GTACATTATC TGCAACACTG GGCTCCACAA TGAAGTCCAC TGAAATCTCT
    TCAACTACCC AATTACCTGT GATCATCAAC AATAATACAG TTTCAGTGGA AGGTCCTGCC
    AATTCTTCAG AAGCTGTTCA GCATTCTGTA AATGAAGGAA TCACAGAGTT ACCTGCTTTG
    GTCTGGCCAG ATATTGAAGG ATCAGATGAA GAATTCAAAG TTTTAGATGA GGACATGGAA
    CTCGTCAAGT TTGTGCCTAC AAAAAGCCCA GTGACTGAAC TCTTAGAAGA AGATTTAGTT
    GGTGATGAAA TATTGGTTGC AACAACAACA ACAACAACAA CTCTATCCTC AACAACAGTA
    GCGACTGTGG TAACAAGTTT AGGTCATTCA GCCTCCCTGA CTCCTGAAAA GGACTCACCT
    TTCACTTGGA TATCACATTC ATCAATAGAT GAGGATGAAC CTGTGCAAGA GTCAACCACA
    GAACCTCTAC CCACCCAATC ACCCACTGCT TCTGTTCTTC CAAAGACAAC TACACAGACA
    TTGATGCACA CATACATAGC AGCTGTTGCA GGGGAGCCTG TTTTATCAGA TAACATGGAT
    AATAAGGACT CAACCACAAT GTCCACCCTT TTAGATGTTT CAAAACATTT AAAGACCCTT
    AGTTCCACCT CTGTACAATC ATTTTCAGAA CCAACATTTA CACCTACTGT CCACATAATT
    AAGCATGGAG AAACTTCAGA TGACTCAGTA CAAGATGTAG TTGAGACTGG AGAAGTTTTA
    GATGACTCTG TACAAGATGT ACTTGAGCAT GAAGACATTT CATATGGCTC TGTACAAGAT
    GTAGTTGTAT CTGGAGAAAC TTCAAATGAC TCAGTACAAG ATGTAGTTGA GACTGGAGAA
    GTTTTAGATG ATTCTGTACA AGATGTACTT GAGCATGAGG ACATTTTATC TGGCCCAGTT
    CAAGATGTAG TTGTGTCTGG AGAAACTGCA GAAGACTCAG TACAAGATTT AGTTGAGACT
    AGAGAAGTTT TAGATGATTC TGTACAAGAT GTACTTGAGC ATGAGGACAT TTTATCTGGC
    CCAGTTCAAG ATGTAGTTGT GTCTGGAGAA ACTGCAGATG ACTCAGTACA AGATTTAGTT
    GAGACTGGAG AAGTTTTAGA TGATTCCGTA CAAGATGTAC TTGAGCATGA AGTAATTTCA
    TTTGGCCCAG TACAAGATGT AGTTGTATCT GGAAAAACTT CAAATGACTC AGTACAAGAT
    GTAGTTGAGA GTGCAAAAGA TTCAGATGCC CCAATACAAG ATGTATTTGA GCATAAAGAC
    ATTTCAGTTG ACTCTGTACA AGATGTAGTT GTGTCTGGAG AAACTGCAGA TGACTCTATT
    CAAGATTTAT TTAAGCATGA AGGCACTTCG TTCGACACAG TACAAGATGA GGTTGAGTCT
    AGAGAAACTT CAGATGACTC AGTACAAAAT GTAGTTGAGC ATGAAGACTC TTCAGATGAC
    GCAGTTCAAG ATGTAGTCGA GTCTGGAGAC ACTTCAGATG ACTCTGTACA AGATATATTT
    GAGTCTGCAG AAACTTCAGA TGACTCTGTA CAAGATGTAG CTGAGACTGG AAAAGAATCA
    GATGACTTAG TACAAGATAT ATTTGAGAAT AAAGACATTT CAGTTGACTC AGTACAAGAT
    GTAGTTGTAT CTGGAGAGAC TTCAGATGAC TCTATTCAAG GTTTACTTGA GCATGAAGGC
    ACTTCAGTCG ACATAGTACA AGATGAGGTT GAGTTTAGAG AAAATTCAGA TGACTCAGTA
    CTAAATGTAG TCAAGCATGA AGATACTTCA GATGACTCAA TACAAGATGT AGTCGAGACA
    GGAGAAGTTT CAGATAGCTC AGTACAAGAT GTAGCTGAGT CTGGAGAAAC TTTAGATGAC
    TCTGTTCAAG ATTTTGTTAA AACTGGAGAA ATTTCCATTG ACTCAGTACA CAATTTATTT
    GAGCCTGAAA AAAACGTAGA TGACTCTGTA CAAGATGTAG TTGAGACTGG AGAAGTTTCT
    GTTGATTCAG TACAAGATGT AATTGAGCTT GGAGACACTT TAGATGACTC TGCACAAGGA
    ATAGTTGAAC CTGAAGAAAT GGCAGACAAC TTTATAAAGG AAAGCACTGT AAAGCCAAGA
    CCTGACATCA AAGGGCTTAT TCTACCAACA GAGACAGTGA ACTATGAAAG TGCCACTATG
    AAAGTTCCTG CTCCAGTTAA CCCCAGCATA ACAGAATTTA AGGTTTCATT TGACATCTTC
    CCTTTCGACG ACCCAACTGA TGATGAAGAT AATGGCAGTG GGTTTGCTCA TGGAACTGAC
    TTAGCAAGTT TAGCCTTGCC TGCCAGTCCT GGAAGGGCAT TAATAGTGTT TTTTAGCCTT
    AGGGTCACCA ACATGAAGTT TTCCGAAGAT CTCTTCAACA AGAGCTCTAC TGAATACAAG
    GCACTTGAGC AGCGATTTCT GGAACTGCTC GTACCGTATC TACAGTCCAA CCTCAGCAAT
    TTCCAGAATT TAGAGATTTT GAACTTCAGG AATGGTAGCA TTGTGGTGAA CAGCAGGATG
    AAGTTTGCCA AGCCAGTTCC CCATGGTGTG ACAACAGCAG TGTACCTAAT CTTGGAGGAC
    TTCTGCAACA CTGCCTACCA AACTATGAAT CTTGCAATAG ACAAGTATTC ATTGGATGTC
    GAATCAGGTG ACCAGGCAGA TCCTTGTAAG TTTCAGGCAT GTAACGAGTT CTCCAAGTGT
    TTGGTGAACC GCTGGTCAGG TGAGGCCGAG TGTGTTTGTA ACGCTGGTTA TTTCAGCGTT
    GACGGACTGC CATGCCAGAG CATCTGCGAC ATCCAAGAAG ACTTCTGCCT AAATGACGGG
    AAATGTGACA TAATACCTGG CAAGGGCGCC ATCTGCAGGT GTCGCGTTGG TGAAAACTGG
    TGGTATCGAG GAGAACACTG TGAGGAATAT GTGTCAGAGC CGCTGGTGGT CGGCATTGCC
    ATTGCGTCCG TTGCTGGATT TCTCCTGGTG GCGTCGGGTG TCATCTTTTT CCTGGCCCGA
    ACTCTGAGAG ACCAGTATGA CACAGATGAT TCAGAAGACC CTTTACGATA TGCAGAGAAC
    TTGCCATCTC TAGAAAGAGC CACCAAGTAC AACCCGATGT TTGAAAGTGA AGTGACTGCA
    GGCCATAATC AGTATTATCG GCGTTACCCA GAAGCTCCGG CATTCAGCAC GGGCAGCACC
    GAGATCTCCA CTGACTTCAG CAGCGAAGAG ATTCGTCACA TCTATCAGAA CAGTGAACTT
    ACTAAAGAGG AAATCCAGGA CCGTATTCGT ATTATAGAGC TTTACGCCAA GGACCGCCAG
    TTTGCAGATT TTGTCCGACA GCACCAAGTG GCGCTTGACG CTAGACGAGA GAGCTCTTCC
    AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45891
    Clone ID Related Accession (Same CDS sequence) XM_021474485.1 , XM_009296448.2 , XM_009296448.3 , XM_021473521.1
    Accession Version XM_021474485.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin homolog isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGCCTGATT TACCTTTTAA ATGCATTTGG TGGGGTTTGA CACTATTGTT GTTGACTGGA 
    TACTTTTCTA TAGATGCAGA TACTGGAACT CATGAGGATT ACTTGGCAAA TGGAAATACA
    ATGCTTTTGG ACACTAATGA AGTTTTGAAT CACTTATTGT TGTTGGACAA AGGTAAAACT
    TTGAATTACA TGTCTGCTGA AGGGAAAACA GCTCTATCTA GACATAAAAG GGACCTCCTG
    TTCCCCAATG GAGTTAAACT GTGCTCACAT GAGACGGTGC AACAGGCAGT GCAGAATCAT
    CTCAACTACT TTCACCTGCG AGTGTGCCAG GAGACAATTT GGGAGGCTTA TAAAATCTTC
    TGGGATCGCT TACCTGAGAA AGAAGAGTAC CAGATCTGGA TGAAAAAATG TCAAAATGGT
    AGCATTAGCG TGTTTGACAT CGGCCGAAGC TTTAGTCAGT CAGCTGAGCA CCTTGCTTTG
    ATTTCAAGTA GGGTAGCATC ATCCACAATA ACATCAACAA GGCATCAGAC GACGACTACT
    TCGCCAACAC TAGCAAAAGC TGAACAAGTT GGTATTTTGA TTCCTGAGGC AGTGCCAGGT
    TCACAAGTTG TCACACCGCA GACCTTTGCA GATGATATCG TAATCACAAC TTTGCCTACA
    AGGCTTCAAG TGACACCTGG AATTACCCCA AAGCCTGATC TTGTCACAGC CTTTAAGGAC
    ACAGTAAAAC CTATTTCTGA AACTGAACCT GAGGTCATAT TGGGTACTAC TTCTGAGAGT
    CCTGTTAAAG CATTGGATAC CATTACCATT AAACATGAAG AGGTTGTTGT AGTCTCAGAA
    TCTAAATTAT TGATTACTGA GAGGACTTCG GAGGACTCTA CTTCAAGGAA ATATGATTAT
    GAGTTTCCCA CGACCTTTAA GGACTCGTTA GTAATAAACA CTCCTATAGT ACTAACAACT
    GAATTTTCTG AACAAATGTA TTCAGAAATA GGACAAGGTT TTTCTGATGG TACAGTTAAA
    ACAGTAGCTG TTACTATTCA AAAGGAACTT GACATTGTCT CAAAATCTAC ATTATTAACC
    ACTGACAAAA CTCCAGAAGT TTCTACTTCA AAAAAATCTG AGCATGATAA TCCCACTAAC
    TTTGTAGATT CATTGGGAAT AAGCACCAAT ACTCCTAGAG TGACCACAAG TGAACTCAAA
    GAAGATCTTC ATTCAGAATT AGAACAGGTG GCTGGGCAGG ATGTTATTCA CAGTCCTGTT
    AAAACAACAC ATGTTACAAT TAAGCATGAA GTGTTGGACA CAAATACTAC ATTAATGACA
    ACTGACAGAA CTCCATATTC TACTTCAGCA AAATATGAAC GTGTTATACC AACATCCTTT
    GAGGACTCAC TAGTCACAAG TTCATCTACT GAACAGGATA TGACCAATGT TCCTATTAAG
    ACGGCATATG CCACAATTTC ACATGGAGAG TCTGAAATTG TCACAGAGTC GACAATAACA
    ACCACTAACA GAAATCTTGA AGATTCTTCT TTAGCAAAAT ATGATTATGG GATTCCTCAA
    GGAATTGATA CTTCTTCGGG AGTAATTAAT GAATCTTCTA CAAAAGAACA TAATTCAGTA
    TCTAAATTGA ATATTGGTCA GACTACTACT GAAGGTCCCC AAACAACTGA TGCAGTTCAG
    TATGTAGAAT CTGACATTGT GTCAGAGGAA ACGTTGAAAT CCACTGGCAG AACTGCAGTT
    AGCTCAAACT CTACAAAAAT TGAGGCCGAT CTTTCCACAA CTCCTGAGGA CCTGACAGGA
    GTAAGTACCA ACACTCCTGA AATAGTCACT AGAGATTCCA TTACAATACT TATCCCAGAA
    ACCGAGGGCA TTACTAAAAA TCCTGTTAAA TCTGCACCTA TTACCATTCA GCATGTGGAT
    GTTGATGATG TTTTAGCAGC TACAGTGATG TCCACTCATA AACCTCCCAA AGTCTCACCT
    ACAGAAATAT CCCCCGATCA GAATCTCTTA CCAGAGGAAT CAAAGCTGCC AACTCCTACA
    AAACTAGCAG AAGTTTTGTC TGCCACAGAA GAGTTGCCAA AGGATCAGAC AGACAAGGAT
    GTTGCTAAAA TTGTATATGT GTCTACTGTG CCAGAACTGG TGTCACCAAC TATTCCCGCA
    TATACGTCAA CTGACACTAC TAAGGAAAAC CTACATGTGT TAACAGGGGG GGCAGTTGTT
    ACATCACTTG ATAAAGATGA GGAAGAACAT ACACCCCACA CAAAAGAGAG CAAAATTACA
    CAGGTGACAA CAGGAGCAGA ATTACCAGGT ACCATAACAG AGAGTACTCA TGGAATGTGG
    AGGGAAACCC AAGAGCCAAT AAATGTCACA CCTGATGCTG AAGACAATCA TTTGGGCAAA
    GAAGGGTACA TAATAACAGA GATCCCAGAA GTTGCTGAAG TACCAGCTGG AGTGACACCC
    AAAATGATTG TGGAAACTAC AACAGCCACT GAATCTGTGT TTACTGTGAA AGACACATCT
    ATAATTGTAG ACAAAACTGG CATTGTGAAA CCTGTCGTGA CTACGACAGA GGCAGCTGAA
    CTTACTGAGG TTGGATTCGA AGATTTAGGC ACAGACAAAG TAACAATTCT TGAAAACACA
    CTTGAAGATT CTGTAGGACT AACAAATGAA CCTTTTGCTG TATCATGGGG TGTAACTGAA
    GCCACTATTT TAGAGGAATC ATATGTGGAA AGTACTGAAA GTATTTCAGG TAAAGAGAAG
    TCCACTGAAA CTATTAAGAT CACAGGTGAA GCCACAACTG ATGACATTGG TACTTCTGAA
    ACTGACATTG TAGTGCCTGT AACCGATCGC ATAGAAGATA ATGTAAATGT ATCACCTAAA
    AATGATGATG CTAAGGAAAC AAACATTTTT GAAGACACAA CACCAGATCT TAGTGTTACT
    AATTCTGAAA AAGACATACT GACAACTACC ATACATAAAG ACATGCATGA AGTTACAGAA
    CCATTAAATA TTCCAGAGGT CACACATAAG GTTGTGATAG AGATCACAGA TGATGTACGT
    GAGACCAGAG GAGATTCCTT ACCCGAGACA CCAGAAGTCA CAACAGAGAC ACCTAAGGTT
    GCAACAGAAG CTGATGTCAA AACAACAACA AATGTTATAT CTAAAGCACC TGAAGTTATA
    CTGAAGACAA CTGTAACCAA GTCAGATACA CCTGATGTCA CAACTCAGAC ACCTTTGTTA
    AAGCTTGAAG ATACAGAGAG GGTTGAAATA GTACCTGAAA CTCCTGACAT ACAAGTGGTT
    CAAACAGAAC ACTCAGAAAA TGATATCCCA ACTCCTGTCT TGAAAGTGGA GGATTTCACA
    GTTGCGCTGA ATGACTTGAC AACTCCTATT CAAAAGACTT CTTCAACCAC ATTACAACAA
    GACATTTCTA ATGATATTTT AGAAGAAAAC AATGTGATGG GAAATGAAAT TGATGATATT
    GTGGTTCGAC CAGTACGCCC TGTCGGCGAT CATATTTTGG AGCTAAGTAT TAAGCTAAGA
    GGGGAAAGCT ATGACGATGC TCTGAGGGAT CCATCAAGTA ACTACTATCA ACATCTGTCT
    ACACTTTTTG TTGAAAAGAT TGAAGAAGTT TTCGAAAGGC TTCCAGGTTT CAAGAAAATA
    TATGTTCTTG AATTCAGGCC ACAGAAGGAC ATTGAAGGAG GTCTGGCAGT GGTGGTGCAC
    TATGCTGTTG TTCTTGAGGG TGATGGGTCA GGAGTAAGTA ATGAGACCAT GGATTACATA
    AACCTGCATT CCAACATGGT TGAGAACTCC TTCACAGATC CTGAGGAGCT GCCCACTGTG
    GTCTACACCA TCACTGACTT CCGAAACTAC ATCTCCGAAG CCCTTCACAA GGATAACTTT
    ATCAGAAACG CTACTCTGAA TGTGGACATT GATTCTTTGC AACTTGAAAA TGTGGAGACA
    TTGCTGCCTT CCAAACCAAC CAGCAGACCG TTTGACTCCA GTGACATGAT GGACAACGTT
    CTTGCTTCCG AGAAACCACC AGATGTTCCT GGGCAAGAAT TATCCAACAA TGACATCTTC
    ATTACAAATT ATGACATCTT TGATCCTGAT CCATGGACAG GTGGACAAAG TGAAGACAGA
    AATGCAAATG ATGTAATCAT CTTTGAGGAA AGTCCCACTC AGTCTCCTGT TGAAGTGTCT
    TTGAAGAACT TGGACATTGA AGTTACTTCA AAATCTGAAA CTTCAAGCAG TGCCCCTGTT
    CCTGATGAAG CTGTTATTGA AGAGGAGGGA TTTTTACAGA CTGCAACTAC AGTCAGTCCT
    ATTATAGACA CAATAACTGA ATTTACTTTA CCTACAGAAT CACCTCACAA GGTAGAAGAG
    ACACCAGTTT CTCCTGTAGA ACCTCCTGAG GTTGAGGAAA CTACCCTGGG TGATTTAACA
    GACCTTGGCT CAGGCTCAGG ATTCTCTGGA GATCACATAG GCCCTGACAT CTGGCCATGG
    TCATCAGAAA CATCACAAGA AGTCTTGAAG GAAGATACCA ATGATGAGAT ACACCAACTT
    CAGGAAAATC AGGAATCTAT AATAAAACAG CTTGAGCCTT TACATCAAGA TGTGACTCCT
    GAGGAGCCAT TCTTGGATAG GGTTCTTGTG ACCCAAGACA TTCGTACCAA TCCTCATTAC
    ACAACAACTG ACCAGGCCCC TGTATTTTGG ACAATGGAGA CTTTGACTGT TGAGCTTTCA
    ATGCAGACTC AAGTAGCCCC TGAACATTAT GATGACTATT TTCCAGATGC ATCAATCACT
    ATGGCTACAC TTGTAACCAA TCAACCATTG TTACATGTTC ACAATCCATT AGAAACAAAA
    GATCTGGATG CATCACAAAG TCCAGGAACA ATAAGTTCAA CTGCTGATAA GGACTATACA
    GAACGACTTG TTACACCCAC TGTGATCCAA CCAAGTGAAA CAGTAGCTGT GGATAAGCAT
    ACTACAACAG ACAACAAACA GAATCTGGAA ACTATGGGTA CATTATCTGC AACACTGGGC
    TCCACAATGA AGTCCACTGA AATCTCTTCA ACTACCCAAT TACCTGTGAT CATCAACAAT
    AATACAGTTT CAGTGGAAGG TCCTGCCAAT TCTTCAGAAG CTGTTCAGCA TTCTGTAAAT
    GAAGGAATCA CAGAGTTACC TGCTTTGGTC TGGCCAGATA TTGAAGGATC AGATGAAGAA
    TTCAAAGTTT TAGATGAGGA CATGGAACTC GTCAAGTTTG TGCCTACAAA AAGCCCAGTG
    ACTGAACTCT TAGAAGAAGA TTTAGTTGGT GATGAAATAT TGGTTGCAAC AACAACAACA
    ACAACAACTC TATCCTCAAC AACAGTAGCG ACTGTGGTAA CAAGTTTAGG TCATTCAGCC
    TCCCTGACTC CTGAAAAGGA CTCACCTTTC ACTTGGATAT CACATTCATC AATAGATGAG
    GATGAACCTG TGCAAGAGTC AACCACAGAA CCTCTACCCA CCCAATCACC CACTGCTTCT
    GTTCTTCCAA AGACAACTAC ACAGACATTG ATGCACACAT ACATAGCAGC TGTTGCAGGG
    GAGCCTGTTT TATCAGATAA CATGGATAAT AAGGACTCAA CCACAATGTC CACCCTTTTA
    GATGTTTCAA AACATTTAAA GACCCTTAGT TCCACCTCTG TACAATCATT TTCAGAACCA
    ACATTTACAC CTACTGTCCA CATAATTAAG CATGGAGAAA CTTCAGATGA CTCAGTACAA
    GATGTAGTTG AGACTGGAGA AGTTTTAGAT GACTCTGTAC AAGATGTACT TGAGCATGAA
    GACATTTCAT ATGGCTCTGT ACAAGATGTA GTTGTATCTG GAGAAACTTC AAATGACTCA
    GTACAAGATG TAGTTGAGAC TGGAGAAGTT TTAGATGATT CTGTACAAGA TGTACTTGAG
    CATGAGGACA TTTTATCTGG CCCAGTTCAA GATGTAGTTG TGTCTGGAGA AACTGCAGAA
    GACTCAGTAC AAGATTTAGT TGAGACTAGA GAAGTTTTAG ATGATTCTGT ACAAGATGTA
    CTTGAGCATG AGGACATTTT ATCTGGCCCA GTTCAAGATG TAGTTGTGTC TGGAGAAACT
    GCAGATGACT CAGTACAAGA TTTAGTTGAG ACTGGAGAAG TTTTAGATGA TTCCGTACAA
    GATGTACTTG AGCATGAAGT AATTTCATTT GGCCCAGTAC AAGATGTAGT TGTATCTGGA
    AAAACTTCAA ATGACTCAGT ACAAGATGTA GTTGAGAGTG CAAAAGATTC AGATGCCCCA
    ATACAAGATG TATTTGAGCA TAAAGACATT TCAGTTGACT CTGTACAAGA TGTAGTTGTG
    TCTGGAGAAA CTGCAGATGA CTCTATTCAA GATTTATTTA AGCATGAAGG CACTTCGTTC
    GACACAGTAC AAGATGAGGT TGAGTCTAGA GAAACTTCAG ATGACTCAGT ACAAAATGTA
    GTTGAGCATG AAGACTCTTC AGATGACGCA GTTCAAGATG TAGTCGAGTC TGGAGACACT
    TCAGATGACT CTGTACAAGA TATATTTGAG TCTGCAGAAA CTTCAGATGA CTCTGTACAA
    GATGTAGCTG AGACTGGAAA AGAATCAGAT GACTTAGTAC AAGATATATT TGAGAATAAA
    GACATTTCAG TTGACTCAGT ACAAGATGTA GTTGTATCTG GAGAGACTTC AGATGACTCT
    ATTCAAGGTT TACTTGAGCA TGAAGGCACT TCAGTCGACA TAGTACAAGA TGAGGTTGAG
    TTTAGAGAAA ATTCAGATGA CTCAGTACTA AATGTAGTCA AGCATGAAGA TACTTCAGAT
    GACTCAATAC AAGATGTAGT CGAGACAGGA GAAGTTTCAG ATAGCTCAGT ACAAGATGTA
    GCTGAGTCTG GAGAAACTTT AGATGACTCT GTTCAAGATT TTGTTAAAAC TGGAGAAATT
    TCCATTGACT CAGTACACAA TTTATTTGAG CCTGAAAAAA ACGTAGATGA CTCTGTACAA
    GATGTAGTTG AGACTGGAGA AGTTTCTGTT GATTCAGTAC AAGATGTAAT TGAGCTTGGA
    GACACTTTAG ATGACTCTGC ACAAGGAATA GTTGAACCTG AAGAAATGGC AGACAACTTT
    ATAAAGGAAA GCACTGTAAA GCCAAGACCT GACATCAAAG GGCTTATTCT ACCAACAGAG
    ACAGTGAACT ATGAAAGTGC CACTATGAAA GTTCCTGCTC CAGTTAACCC CAGCATAACA
    GAATTTAAGG TTTCATTTGA CATCTTCCCT TTCGACGACC CAACTGATGA TGAAGATAAT
    GGCAGTGGGT TTGCTCATGG AACTGACTTA GCAAGTTTAG CCTTGCCTGC CAGTCCTGGA
    AGGGCATTAA TAGTGTTTTT TAGCCTTAGG GTCACCAACA TGAAGTTTTC CGAAGATCTC
    TTCAACAAGA GCTCTACTGA ATACAAGGCA CTTGAGCAGC GATTTCTGGA ACTGCTCGTA
    CCGTATCTAC AGTCCAACCT CAGCAATTTC CAGAATTTAG AGATTTTGAA CTTCAGGAAT
    GGTAGCATTG TGGTGAACAG CAGGATGAAG TTTGCCAAGC CAGTTCCCCA TGGTGTGACA
    ACAGCAGTGT ACCTAATCTT GGAGGACTTC TGCAACACTG CCTACCAAAC TATGAATCTT
    GCAATAGACA AGTATTCATT GGATGTCGAA TCAGGTGACC AGGCAGATCC TTGTAAGTTT
    CAGGCATGTA ACGAGTTCTC CAAGTGTTTG GTGAACCGCT GGTCAGGTGA GGCCGAGTGT
    GTTTGTAACG CTGGTTATTT CAGCGTTGAC GGACTGCCAT GCCAGAGCAT CTGCGACATC
    CAAGAAGACT TCTGCCTAAA TGACGGGAAA TGTGACATAA TACCTGGCAA GGGCGCCATC
    TGCAGGTGTC GCGTTGGTGA AAACTGGTGG TATCGAGGAG AACACTGTGA GGAATATGTG
    TCAGAGCCGC TGGTGGTCGG CATTGCCATT GCGTCCGTTG CTGGATTTCT CCTGGTGGCG
    TCGGGTGTCA TCTTTTTCCT GGCCCGAACT CTGAGAGACC AGTATGACAC AGATGATTCA
    GAAGACCCTT TACGATATGC AGAGAACTTG CCATCTCTAG AAAGAGCCAC CAAGTACAAC
    CCGATGTTTG AAAGTGAAGT GACTGCAGGC CATAATCAGT ATTATCGGCG TTACCCAGAA
    GCTCCGGCAT TCAGCACGGG CAGCACCGAG ATCTCCACTG ACTTCAGCAG CGAAGAGATT
    CGTCACATCT ATCAGAACAG TGAACTTACT AAAGAGGAAA TCCAGGACCG TATTCGTATT
    ATAGAGCTTT ACGCCAAGGA CCGCCAGTTT GCAGATTTTG TCCGACAGCA CCAAGTGGCG
    CTTGACGCTA GACGAGAGAG CTCTTCCAAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330160.1
    CDS233..8665
    Translation

    Target ORF information:

    RefSeq Version XM_021474485.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio interphotoreceptor matrix proteoglycan 2b (impg2b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474485.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGCCTGATT TACCTTTTAA ATGCATTTGG TGGGGTTTGA CACTATTGTT GTTGACTGGA 
    TACTTTTCTA TAGATGCAGA TACTGGAACT CATGAGGATT ACTTGGCAAA TGGAAATACA
    ATGCTTTTGG ACACTAATGA AGTTTTGAAT CACTTATTGT TGTTGGACAA AGGTAAAACT
    TTGAATTACA TGTCTGCTGA AGGGAAAACA GCTCTATCTA GACATAAAAG GGACCTCCTG
    TTCCCCAATG GAGTTAAACT GTGCTCACAT GAGACGGTGC AACAGGCAGT GCAGAATCAT
    CTCAACTACT TTCACCTGCG AGTGTGCCAG GAGACAATTT GGGAGGCTTA TAAAATCTTC
    TGGGATCGCT TACCTGAGAA AGAAGAGTAC CAGATCTGGA TGAAAAAATG TCAAAATGGT
    AGCATTAGCG TGTTTGACAT CGGCCGAAGC TTTAGTCAGT CAGCTGAGCA CCTTGCTTTG
    ATTTCAAGTA GGGTAGCATC ATCCACAATA ACATCAACAA GGCATCAGAC GACGACTACT
    TCGCCAACAC TAGCAAAAGC TGAACAAGTT GGTATTTTGA TTCCTGAGGC AGTGCCAGGT
    TCACAAGTTG TCACACCGCA GACCTTTGCA GATGATATCG TAATCACAAC TTTGCCTACA
    AGGCTTCAAG TGACACCTGG AATTACCCCA AAGCCTGATC TTGTCACAGC CTTTAAGGAC
    ACAGTAAAAC CTATTTCTGA AACTGAACCT GAGGTCATAT TGGGTACTAC TTCTGAGAGT
    CCTGTTAAAG CATTGGATAC CATTACCATT AAACATGAAG AGGTTGTTGT AGTCTCAGAA
    TCTAAATTAT TGATTACTGA GAGGACTTCG GAGGACTCTA CTTCAAGGAA ATATGATTAT
    GAGTTTCCCA CGACCTTTAA GGACTCGTTA GTAATAAACA CTCCTATAGT ACTAACAACT
    GAATTTTCTG AACAAATGTA TTCAGAAATA GGACAAGGTT TTTCTGATGG TACAGTTAAA
    ACAGTAGCTG TTACTATTCA AAAGGAACTT GACATTGTCT CAAAATCTAC ATTATTAACC
    ACTGACAAAA CTCCAGAAGT TTCTACTTCA AAAAAATCTG AGCATGATAA TCCCACTAAC
    TTTGTAGATT CATTGGGAAT AAGCACCAAT ACTCCTAGAG TGACCACAAG TGAACTCAAA
    GAAGATCTTC ATTCAGAATT AGAACAGGTG GCTGGGCAGG ATGTTATTCA CAGTCCTGTT
    AAAACAACAC ATGTTACAAT TAAGCATGAA GTGTTGGACA CAAATACTAC ATTAATGACA
    ACTGACAGAA CTCCATATTC TACTTCAGCA AAATATGAAC GTGTTATACC AACATCCTTT
    GAGGACTCAC TAGTCACAAG TTCATCTACT GAACAGGATA TGACCAATGT TCCTATTAAG
    ACGGCATATG CCACAATTTC ACATGGAGAG TCTGAAATTG TCACAGAGTC GACAATAACA
    ACCACTAACA GAAATCTTGA AGATTCTTCT TTAGCAAAAT ATGATTATGG GATTCCTCAA
    GGAATTGATA CTTCTTCGGG AGTAATTAAT GAATCTTCTA CAAAAGAACA TAATTCAGTA
    TCTAAATTGA ATATTGGTCA GACTACTACT GAAGGTCCCC AAACAACTGA TGCAGTTCAG
    TATGTAGAAT CTGACATTGT GTCAGAGGAA ACGTTGAAAT CCACTGGCAG AACTGCAGTT
    AGCTCAAACT CTACAAAAAT TGAGGCCGAT CTTTCCACAA CTCCTGAGGA CCTGACAGGA
    GTAAGTACCA ACACTCCTGA AATAGTCACT AGAGATTCCA TTACAATACT TATCCCAGAA
    ACCGAGGGCA TTACTAAAAA TCCTGTTAAA TCTGCACCTA TTACCATTCA GCATGTGGAT
    GTTGATGATG TTTTAGCAGC TACAGTGATG TCCACTCATA AACCTCCCAA AGTCTCACCT
    ACAGAAATAT CCCCCGATCA GAATCTCTTA CCAGAGGAAT CAAAGCTGCC AACTCCTACA
    AAACTAGCAG AAGTTTTGTC TGCCACAGAA GAGTTGCCAA AGGATCAGAC AGACAAGGAT
    GTTGCTAAAA TTGTATATGT GTCTACTGTG CCAGAACTGG TGTCACCAAC TATTCCCGCA
    TATACGTCAA CTGACACTAC TAAGGAAAAC CTACATGTGT TAACAGGGGG GGCAGTTGTT
    ACATCACTTG ATAAAGATGA GGAAGAACAT ACACCCCACA CAAAAGAGAG CAAAATTACA
    CAGGTGACAA CAGGAGCAGA ATTACCAGGT ACCATAACAG AGAGTACTCA TGGAATGTGG
    AGGGAAACCC AAGAGCCAAT AAATGTCACA CCTGATGCTG AAGACAATCA TTTGGGCAAA
    GAAGGGTACA TAATAACAGA GATCCCAGAA GTTGCTGAAG TACCAGCTGG AGTGACACCC
    AAAATGATTG TGGAAACTAC AACAGCCACT GAATCTGTGT TTACTGTGAA AGACACATCT
    ATAATTGTAG ACAAAACTGG CATTGTGAAA CCTGTCGTGA CTACGACAGA GGCAGCTGAA
    CTTACTGAGG TTGGATTCGA AGATTTAGGC ACAGACAAAG TAACAATTCT TGAAAACACA
    CTTGAAGATT CTGTAGGACT AACAAATGAA CCTTTTGCTG TATCATGGGG TGTAACTGAA
    GCCACTATTT TAGAGGAATC ATATGTGGAA AGTACTGAAA GTATTTCAGG TAAAGAGAAG
    TCCACTGAAA CTATTAAGAT CACAGGTGAA GCCACAACTG ATGACATTGG TACTTCTGAA
    ACTGACATTG TAGTGCCTGT AACCGATCGC ATAGAAGATA ATGTAAATGT ATCACCTAAA
    AATGATGATG CTAAGGAAAC AAACATTTTT GAAGACACAA CACCAGATCT TAGTGTTACT
    AATTCTGAAA AAGACATACT GACAACTACC ATACATAAAG ACATGCATGA AGTTACAGAA
    CCATTAAATA TTCCAGAGGT CACACATAAG GTTGTGATAG AGATCACAGA TGATGTACGT
    GAGACCAGAG GAGATTCCTT ACCCGAGACA CCAGAAGTCA CAACAGAGAC ACCTAAGGTT
    GCAACAGAAG CTGATGTCAA AACAACAACA AATGTTATAT CTAAAGCACC TGAAGTTATA
    CTGAAGACAA CTGTAACCAA GTCAGATACA CCTGATGTCA CAACTCAGAC ACCTTTGTTA
    AAGCTTGAAG ATACAGAGAG GGTTGAAATA GTACCTGAAA CTCCTGACAT ACAAGTGGTT
    CAAACAGAAC ACTCAGAAAA TGATATCCCA ACTCCTGTCT TGAAAGTGGA GGATTTCACA
    GTTGCGCTGA ATGACTTGAC AACTCCTATT CAAAAGACTT CTTCAACCAC ATTACAACAA
    GACATTTCTA ATGATATTTT AGAAGAAAAC AATGTGATGG GAAATGAAAT TGATGATATT
    GTGGTTCGAC CAGTACGCCC TGTCGGCGAT CATATTTTGG AGCTAAGTAT TAAGCTAAGA
    GGGGAAAGCT ATGACGATGC TCTGAGGGAT CCATCAAGTA ACTACTATCA ACATCTGTCT
    ACACTTTTTG TTGAAAAGAT TGAAGAAGTT TTCGAAAGGC TTCCAGGTTT CAAGAAAATA
    TATGTTCTTG AATTCAGGCC ACAGAAGGAC ATTGAAGGAG GTCTGGCAGT GGTGGTGCAC
    TATGCTGTTG TTCTTGAGGG TGATGGGTCA GGAGTAAGTA ATGAGACCAT GGATTACATA
    AACCTGCATT CCAACATGGT TGAGAACTCC TTCACAGATC CTGAGGAGCT GCCCACTGTG
    GTCTACACCA TCACTGACTT CCGAAACTAC ATCTCCGAAG CCCTTCACAA GGATAACTTT
    ATCAGAAACG CTACTCTGAA TGTGGACATT GATTCTTTGC AACTTGAAAA TGTGGAGACA
    TTGCTGCCTT CCAAACCAAC CAGCAGACCG TTTGACTCCA GTGACATGAT GGACAACGTT
    CTTGCTTCCG AGAAACCACC AGATGTTCCT GGGCAAGAAT TATCCAACAA TGACATCTTC
    ATTACAAATT ATGACATCTT TGATCCTGAT CCATGGACAG GTGGACAAAG TGAAGACAGA
    AATGCAAATG ATGTAATCAT CTTTGAGGAA AGTCCCACTC AGTCTCCTGT TGAAGTGTCT
    TTGAAGAACT TGGACATTGA AGTTACTTCA AAATCTGAAA CTTCAAGCAG TGCCCCTGTT
    CCTGATGAAG CTGTTATTGA AGAGGAGGGA TTTTTACAGA CTGCAACTAC AGTCAGTCCT
    ATTATAGACA CAATAACTGA ATTTACTTTA CCTACAGAAT CACCTCACAA GGTAGAAGAG
    ACACCAGTTT CTCCTGTAGA ACCTCCTGAG GTTGAGGAAA CTACCCTGGG TGATTTAACA
    GACCTTGGCT CAGGCTCAGG ATTCTCTGGA GATCACATAG GCCCTGACAT CTGGCCATGG
    TCATCAGAAA CATCACAAGA AGTCTTGAAG GAAGATACCA ATGATGAGAT ACACCAACTT
    CAGGAAAATC AGGAATCTAT AATAAAACAG CTTGAGCCTT TACATCAAGA TGTGACTCCT
    GAGGAGCCAT TCTTGGATAG GGTTCTTGTG ACCCAAGACA TTCGTACCAA TCCTCATTAC
    ACAACAACTG ACCAGGCCCC TGTATTTTGG ACAATGGAGA CTTTGACTGT TGAGCTTTCA
    ATGCAGACTC AAGTAGCCCC TGAACATTAT GATGACTATT TTCCAGATGC ATCAATCACT
    ATGGCTACAC TTGTAACCAA TCAACCATTG TTACATGTTC ACAATCCATT AGAAACAAAA
    GATCTGGATG CATCACAAAG TCCAGGAACA ATAAGTTCAA CTGCTGATAA GGACTATACA
    GAACGACTTG TTACACCCAC TGTGATCCAA CCAAGTGAAA CAGTAGCTGT GGATAAGCAT
    ACTACAACAG ACAACAAACA GAATCTGGAA ACTATGGGTA CATTATCTGC AACACTGGGC
    TCCACAATGA AGTCCACTGA AATCTCTTCA ACTACCCAAT TACCTGTGAT CATCAACAAT
    AATACAGTTT CAGTGGAAGG TCCTGCCAAT TCTTCAGAAG CTGTTCAGCA TTCTGTAAAT
    GAAGGAATCA CAGAGTTACC TGCTTTGGTC TGGCCAGATA TTGAAGGATC AGATGAAGAA
    TTCAAAGTTT TAGATGAGGA CATGGAACTC GTCAAGTTTG TGCCTACAAA AAGCCCAGTG
    ACTGAACTCT TAGAAGAAGA TTTAGTTGGT GATGAAATAT TGGTTGCAAC AACAACAACA
    ACAACAACTC TATCCTCAAC AACAGTAGCG ACTGTGGTAA CAAGTTTAGG TCATTCAGCC
    TCCCTGACTC CTGAAAAGGA CTCACCTTTC ACTTGGATAT CACATTCATC AATAGATGAG
    GATGAACCTG TGCAAGAGTC AACCACAGAA CCTCTACCCA CCCAATCACC CACTGCTTCT
    GTTCTTCCAA AGACAACTAC ACAGACATTG ATGCACACAT ACATAGCAGC TGTTGCAGGG
    GAGCCTGTTT TATCAGATAA CATGGATAAT AAGGACTCAA CCACAATGTC CACCCTTTTA
    GATGTTTCAA AACATTTAAA GACCCTTAGT TCCACCTCTG TACAATCATT TTCAGAACCA
    ACATTTACAC CTACTGTCCA CATAATTAAG CATGGAGAAA CTTCAGATGA CTCAGTACAA
    GATGTAGTTG AGACTGGAGA AGTTTTAGAT GACTCTGTAC AAGATGTACT TGAGCATGAA
    GACATTTCAT ATGGCTCTGT ACAAGATGTA GTTGTATCTG GAGAAACTTC AAATGACTCA
    GTACAAGATG TAGTTGAGAC TGGAGAAGTT TTAGATGATT CTGTACAAGA TGTACTTGAG
    CATGAGGACA TTTTATCTGG CCCAGTTCAA GATGTAGTTG TGTCTGGAGA AACTGCAGAA
    GACTCAGTAC AAGATTTAGT TGAGACTAGA GAAGTTTTAG ATGATTCTGT ACAAGATGTA
    CTTGAGCATG AGGACATTTT ATCTGGCCCA GTTCAAGATG TAGTTGTGTC TGGAGAAACT
    GCAGATGACT CAGTACAAGA TTTAGTTGAG ACTGGAGAAG TTTTAGATGA TTCCGTACAA
    GATGTACTTG AGCATGAAGT AATTTCATTT GGCCCAGTAC AAGATGTAGT TGTATCTGGA
    AAAACTTCAA ATGACTCAGT ACAAGATGTA GTTGAGAGTG CAAAAGATTC AGATGCCCCA
    ATACAAGATG TATTTGAGCA TAAAGACATT TCAGTTGACT CTGTACAAGA TGTAGTTGTG
    TCTGGAGAAA CTGCAGATGA CTCTATTCAA GATTTATTTA AGCATGAAGG CACTTCGTTC
    GACACAGTAC AAGATGAGGT TGAGTCTAGA GAAACTTCAG ATGACTCAGT ACAAAATGTA
    GTTGAGCATG AAGACTCTTC AGATGACGCA GTTCAAGATG TAGTCGAGTC TGGAGACACT
    TCAGATGACT CTGTACAAGA TATATTTGAG TCTGCAGAAA CTTCAGATGA CTCTGTACAA
    GATGTAGCTG AGACTGGAAA AGAATCAGAT GACTTAGTAC AAGATATATT TGAGAATAAA
    GACATTTCAG TTGACTCAGT ACAAGATGTA GTTGTATCTG GAGAGACTTC AGATGACTCT
    ATTCAAGGTT TACTTGAGCA TGAAGGCACT TCAGTCGACA TAGTACAAGA TGAGGTTGAG
    TTTAGAGAAA ATTCAGATGA CTCAGTACTA AATGTAGTCA AGCATGAAGA TACTTCAGAT
    GACTCAATAC AAGATGTAGT CGAGACAGGA GAAGTTTCAG ATAGCTCAGT ACAAGATGTA
    GCTGAGTCTG GAGAAACTTT AGATGACTCT GTTCAAGATT TTGTTAAAAC TGGAGAAATT
    TCCATTGACT CAGTACACAA TTTATTTGAG CCTGAAAAAA ACGTAGATGA CTCTGTACAA
    GATGTAGTTG AGACTGGAGA AGTTTCTGTT GATTCAGTAC AAGATGTAAT TGAGCTTGGA
    GACACTTTAG ATGACTCTGC ACAAGGAATA GTTGAACCTG AAGAAATGGC AGACAACTTT
    ATAAAGGAAA GCACTGTAAA GCCAAGACCT GACATCAAAG GGCTTATTCT ACCAACAGAG
    ACAGTGAACT ATGAAAGTGC CACTATGAAA GTTCCTGCTC CAGTTAACCC CAGCATAACA
    GAATTTAAGG TTTCATTTGA CATCTTCCCT TTCGACGACC CAACTGATGA TGAAGATAAT
    GGCAGTGGGT TTGCTCATGG AACTGACTTA GCAAGTTTAG CCTTGCCTGC CAGTCCTGGA
    AGGGCATTAA TAGTGTTTTT TAGCCTTAGG GTCACCAACA TGAAGTTTTC CGAAGATCTC
    TTCAACAAGA GCTCTACTGA ATACAAGGCA CTTGAGCAGC GATTTCTGGA ACTGCTCGTA
    CCGTATCTAC AGTCCAACCT CAGCAATTTC CAGAATTTAG AGATTTTGAA CTTCAGGAAT
    GGTAGCATTG TGGTGAACAG CAGGATGAAG TTTGCCAAGC CAGTTCCCCA TGGTGTGACA
    ACAGCAGTGT ACCTAATCTT GGAGGACTTC TGCAACACTG CCTACCAAAC TATGAATCTT
    GCAATAGACA AGTATTCATT GGATGTCGAA TCAGGTGACC AGGCAGATCC TTGTAAGTTT
    CAGGCATGTA ACGAGTTCTC CAAGTGTTTG GTGAACCGCT GGTCAGGTGA GGCCGAGTGT
    GTTTGTAACG CTGGTTATTT CAGCGTTGAC GGACTGCCAT GCCAGAGCAT CTGCGACATC
    CAAGAAGACT TCTGCCTAAA TGACGGGAAA TGTGACATAA TACCTGGCAA GGGCGCCATC
    TGCAGGTGTC GCGTTGGTGA AAACTGGTGG TATCGAGGAG AACACTGTGA GGAATATGTG
    TCAGAGCCGC TGGTGGTCGG CATTGCCATT GCGTCCGTTG CTGGATTTCT CCTGGTGGCG
    TCGGGTGTCA TCTTTTTCCT GGCCCGAACT CTGAGAGACC AGTATGACAC AGATGATTCA
    GAAGACCCTT TACGATATGC AGAGAACTTG CCATCTCTAG AAAGAGCCAC CAAGTACAAC
    CCGATGTTTG AAAGTGAAGT GACTGCAGGC CATAATCAGT ATTATCGGCG TTACCCAGAA
    GCTCCGGCAT TCAGCACGGG CAGCACCGAG ATCTCCACTG ACTTCAGCAG CGAAGAGATT
    CGTCACATCT ATCAGAACAG TGAACTTACT AAAGAGGAAA TCCAGGACCG TATTCGTATT
    ATAGAGCTTT ACGCCAAGGA CCGCCAGTTT GCAGATTTTG TCCGACAGCA CCAAGTGGCG
    CTTGACGCTA GACGAGAGAG CTCTTCCAAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45891
    Clone ID Related Accession (Same CDS sequence) XM_021474485.1 , XM_009296448.2 , XM_009296448.3 , XM_021473521.1
    Accession Version XM_009296448.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product titin homolog isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296448.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGCCTGATT TACCTTTTAA ATGCATTTGG TGGGGTTTGA CACTATTGTT GTTGACTGGA 
    TACTTTTCTA TAGATGCAGA TACTGGAACT CATGAGGATT ACTTGGCAAA TGGAAATACA
    ATGCTTTTGG ACACTAATGA AGTTTTGAAT CACTTATTGT TGTTGGACAA AGGTAAAACT
    TTGAATTACA TGTCTGCTGA AGGGAAAACA GCTCTATCTA GACATAAAAG GGACCTCCTG
    TTCCCCAATG GAGTTAAACT GTGCTCACAT GAGACGGTGC AACAGGCAGT GCAGAATCAT
    CTCAACTACT TTCACCTGCG AGTGTGCCAG GAGACAATTT GGGAGGCTTA TAAAATCTTC
    TGGGATCGCT TACCTGAGAA AGAAGAGTAC CAGATCTGGA TGAAAAAATG TCAAAATGGT
    AGCATTAGCG TGTTTGACAT CGGCCGAAGC TTTAGTCAGT CAGCTGAGCA CCTTGCTTTG
    ATTTCAAGTA GGGTAGCATC ATCCACAATA ACATCAACAA GGCATCAGAC GACGACTACT
    TCGCCAACAC TAGCAAAAGC TGAACAAGTT GGTATTTTGA TTCCTGAGGC AGTGCCAGGT
    TCACAAGTTG TCACACCGCA GACCTTTGCA GATGATATCG TAATCACAAC TTTGCCTACA
    AGGCTTCAAG TGACACCTGG AATTACCCCA AAGCCTGATC TTGTCACAGC CTTTAAGGAC
    ACAGTAAAAC CTATTTCTGA AACTGAACCT GAGGTCATAT TGGGTACTAC TTCTGAGAGT
    CCTGTTAAAG CATTGGATAC CATTACCATT AAACATGAAG AGGTTGTTGT AGTCTCAGAA
    TCTAAATTAT TGATTACTGA GAGGACTTCG GAGGACTCTA CTTCAAGGAA ATATGATTAT
    GAGTTTCCCA CGACCTTTAA GGACTCGTTA GTAATAAACA CTCCTATAGT ACTAACAACT
    GAATTTTCTG AACAAATGTA TTCAGAAATA GGACAAGGTT TTTCTGATGG TACAGTTAAA
    ACAGTAGCTG TTACTATTCA AAAGGAACTT GACATTGTCT CAAAATCTAC ATTATTAACC
    ACTGACAAAA CTCCAGAAGT TTCTACTTCA AAAAAATCTG AGCATGATAA TCCCACTAAC
    TTTGTAGATT CATTGGGAAT AAGCACCAAT ACTCCTAGAG TGACCACAAG TGAACTCAAA
    GAAGATCTTC ATTCAGAATT AGAACAGGTG GCTGGGCAGG ATGTTATTCA CAGTCCTGTT
    AAAACAACAC ATGTTACAAT TAAGCATGAA GTGTTGGACA CAAATACTAC ATTAATGACA
    ACTGACAGAA CTCCATATTC TACTTCAGCA AAATATGAAC GTGTTATACC AACATCCTTT
    GAGGACTCAC TAGTCACAAG TTCATCTACT GAACAGGATA TGACCAATGT TCCTATTAAG
    ACGGCATATG CCACAATTTC ACATGGAGAG TCTGAAATTG TCACAGAGTC GACAATAACA
    ACCACTAACA GAAATCTTGA AGATTCTTCT TTAGCAAAAT ATGATTATGG GATTCCTCAA
    GGAATTGATA CTTCTTCGGG AGTAATTAAT GAATCTTCTA CAAAAGAACA TAATTCAGTA
    TCTAAATTGA ATATTGGTCA GACTACTACT GAAGGTCCCC AAACAACTGA TGCAGTTCAG
    TATGTAGAAT CTGACATTGT GTCAGAGGAA ACGTTGAAAT CCACTGGCAG AACTGCAGTT
    AGCTCAAACT CTACAAAAAT TGAGGCCGAT CTTTCCACAA CTCCTGAGGA CCTGACAGGA
    GTAAGTACCA ACACTCCTGA AATAGTCACT AGAGATTCCA TTACAATACT TATCCCAGAA
    ACCGAGGGCA TTACTAAAAA TCCTGTTAAA TCTGCACCTA TTACCATTCA GCATGTGGAT
    GTTGATGATG TTTTAGCAGC TACAGTGATG TCCACTCATA AACCTCCCAA AGTCTCACCT
    ACAGAAATAT CCCCCGATCA GAATCTCTTA CCAGAGGAAT CAAAGCTGCC AACTCCTACA
    AAACTAGCAG AAGTTTTGTC TGCCACAGAA GAGTTGCCAA AGGATCAGAC AGACAAGGAT
    GTTGCTAAAA TTGTATATGT GTCTACTGTG CCAGAACTGG TGTCACCAAC TATTCCCGCA
    TATACGTCAA CTGACACTAC TAAGGAAAAC CTACATGTGT TAACAGGGGG GGCAGTTGTT
    ACATCACTTG ATAAAGATGA GGAAGAACAT ACACCCCACA CAAAAGAGAG CAAAATTACA
    CAGGTGACAA CAGGAGCAGA ATTACCAGGT ACCATAACAG AGAGTACTCA TGGAATGTGG
    AGGGAAACCC AAGAGCCAAT AAATGTCACA CCTGATGCTG AAGACAATCA TTTGGGCAAA
    GAAGGGTACA TAATAACAGA GATCCCAGAA GTTGCTGAAG TACCAGCTGG AGTGACACCC
    AAAATGATTG TGGAAACTAC AACAGCCACT GAATCTGTGT TTACTGTGAA AGACACATCT
    ATAATTGTAG ACAAAACTGG CATTGTGAAA CCTGTCGTGA CTACGACAGA GGCAGCTGAA
    CTTACTGAGG TTGGATTCGA AGATTTAGGC ACAGACAAAG TAACAATTCT TGAAAACACA
    CTTGAAGATT CTGTAGGACT AACAAATGAA CCTTTTGCTG TATCATGGGG TGTAACTGAA
    GCCACTATTT TAGAGGAATC ATATGTGGAA AGTACTGAAA GTATTTCAGG TAAAGAGAAG
    TCCACTGAAA CTATTAAGAT CACAGGTGAA GCCACAACTG ATGACATTGG TACTTCTGAA
    ACTGACATTG TAGTGCCTGT AACCGATCGC ATAGAAGATA ATGTAAATGT ATCACCTAAA
    AATGATGATG CTAAGGAAAC AAACATTTTT GAAGACACAA CACCAGATCT TAGTGTTACT
    AATTCTGAAA AAGACATACT GACAACTACC ATACATAAAG ACATGCATGA AGTTACAGAA
    CCATTAAATA TTCCAGAGGT CACACATAAG GTTGTGATAG AGATCACAGA TGATGTACGT
    GAGACCAGAG GAGATTCCTT ACCCGAGACA CCAGAAGTCA CAACAGAGAC ACCTAAGGTT
    GCAACAGAAG CTGATGTCAA AACAACAACA AATGTTATAT CTAAAGCACC TGAAGTTATA
    CTGAAGACAA CTGTAACCAA GTCAGATACA CCTGATGTCA CAACTCAGAC ACCTTTGTTA
    AAGCTTGAAG ATACAGAGAG GGTTGAAATA GTACCTGAAA CTCCTGACAT ACAAGTGGTT
    CAAACAGAAC ACTCAGAAAA TGATATCCCA ACTCCTGTCT TGAAAGTGGA GGATTTCACA
    GTTGCGCTGA ATGACTTGAC AACTCCTATT CAAAAGACTT CTTCAACCAC ATTACAACAA
    GACATTTCTA ATGATATTTT AGAAGAAAAC AATGTGATGG GAAATGAAAT TGATGATATT
    GTGGTTCGAC CAGTACGCCC TGTCGGCGAT CATATTTTGG AGCTAAGTAT TAAGCTAAGA
    GGGGAAAGCT ATGACGATGC TCTGAGGGAT CCATCAAGTA ACTACTATCA ACATCTGTCT
    ACACTTTTTG TTGAAAAGAT TGAAGAAGTT TTCGAAAGGC TTCCAGGTTT CAAGAAAATA
    TATGTTCTTG AATTCAGGCC ACAGAAGGAC ATTGAAGGAG GTCTGGCAGT GGTGGTGCAC
    TATGCTGTTG TTCTTGAGGG TGATGGGTCA GGAGTAAGTA ATGAGACCAT GGATTACATA
    AACCTGCATT CCAACATGGT TGAGAACTCC TTCACAGATC CTGAGGAGCT GCCCACTGTG
    GTCTACACCA TCACTGACTT CCGAAACTAC ATCTCCGAAG CCCTTCACAA GGATAACTTT
    ATCAGAAACG CTACTCTGAA TGTGGACATT GATTCTTTGC AACTTGAAAA TGTGGAGACA
    TTGCTGCCTT CCAAACCAAC CAGCAGACCG TTTGACTCCA GTGACATGAT GGACAACGTT
    CTTGCTTCCG AGAAACCACC AGATGTTCCT GGGCAAGAAT TATCCAACAA TGACATCTTC
    ATTACAAATT ATGACATCTT TGATCCTGAT CCATGGACAG GTGGACAAAG TGAAGACAGA
    AATGCAAATG ATGTAATCAT CTTTGAGGAA AGTCCCACTC AGTCTCCTGT TGAAGTGTCT
    TTGAAGAACT TGGACATTGA AGTTACTTCA AAATCTGAAA CTTCAAGCAG TGCCCCTGTT
    CCTGATGAAG CTGTTATTGA AGAGGAGGGA TTTTTACAGA CTGCAACTAC AGTCAGTCCT
    ATTATAGACA CAATAACTGA ATTTACTTTA CCTACAGAAT CACCTCACAA GGTAGAAGAG
    ACACCAGTTT CTCCTGTAGA ACCTCCTGAG GTTGAGGAAA CTACCCTGGG TGATTTAACA
    GACCTTGGCT CAGGCTCAGG ATTCTCTGGA GATCACATAG GCCCTGACAT CTGGCCATGG
    TCATCAGAAA CATCACAAGA AGTCTTGAAG GAAGATACCA ATGATGAGAT ACACCAACTT
    CAGGAAAATC AGGAATCTAT AATAAAACAG CTTGAGCCTT TACATCAAGA TGTGACTCCT
    GAGGAGCCAT TCTTGGATAG GGTTCTTGTG ACCCAAGACA TTCGTACCAA TCCTCATTAC
    ACAACAACTG ACCAGGCCCC TGTATTTTGG ACAATGGAGA CTTTGACTGT TGAGCTTTCA
    ATGCAGACTC AAGTAGCCCC TGAACATTAT GATGACTATT TTCCAGATGC ATCAATCACT
    ATGGCTACAC TTGTAACCAA TCAACCATTG TTACATGTTC ACAATCCATT AGAAACAAAA
    GATCTGGATG CATCACAAAG TCCAGGAACA ATAAGTTCAA CTGCTGATAA GGACTATACA
    GAACGACTTG TTACACCCAC TGTGATCCAA CCAAGTGAAA CAGTAGCTGT GGATAAGCAT
    ACTACAACAG ACAACAAACA GAATCTGGAA ACTATGGGTA CATTATCTGC AACACTGGGC
    TCCACAATGA AGTCCACTGA AATCTCTTCA ACTACCCAAT TACCTGTGAT CATCAACAAT
    AATACAGTTT CAGTGGAAGG TCCTGCCAAT TCTTCAGAAG CTGTTCAGCA TTCTGTAAAT
    GAAGGAATCA CAGAGTTACC TGCTTTGGTC TGGCCAGATA TTGAAGGATC AGATGAAGAA
    TTCAAAGTTT TAGATGAGGA CATGGAACTC GTCAAGTTTG TGCCTACAAA AAGCCCAGTG
    ACTGAACTCT TAGAAGAAGA TTTAGTTGGT GATGAAATAT TGGTTGCAAC AACAACAACA
    ACAACAACTC TATCCTCAAC AACAGTAGCG ACTGTGGTAA CAAGTTTAGG TCATTCAGCC
    TCCCTGACTC CTGAAAAGGA CTCACCTTTC ACTTGGATAT CACATTCATC AATAGATGAG
    GATGAACCTG TGCAAGAGTC AACCACAGAA CCTCTACCCA CCCAATCACC CACTGCTTCT
    GTTCTTCCAA AGACAACTAC ACAGACATTG ATGCACACAT ACATAGCAGC TGTTGCAGGG
    GAGCCTGTTT TATCAGATAA CATGGATAAT AAGGACTCAA CCACAATGTC CACCCTTTTA
    GATGTTTCAA AACATTTAAA GACCCTTAGT TCCACCTCTG TACAATCATT TTCAGAACCA
    ACATTTACAC CTACTGTCCA CATAATTAAG CATGGAGAAA CTTCAGATGA CTCAGTACAA
    GATGTAGTTG AGACTGGAGA AGTTTTAGAT GACTCTGTAC AAGATGTACT TGAGCATGAA
    GACATTTCAT ATGGCTCTGT ACAAGATGTA GTTGTATCTG GAGAAACTTC AAATGACTCA
    GTACAAGATG TAGTTGAGAC TGGAGAAGTT TTAGATGATT CTGTACAAGA TGTACTTGAG
    CATGAGGACA TTTTATCTGG CCCAGTTCAA GATGTAGTTG TGTCTGGAGA AACTGCAGAA
    GACTCAGTAC AAGATTTAGT TGAGACTAGA GAAGTTTTAG ATGATTCTGT ACAAGATGTA
    CTTGAGCATG AGGACATTTT ATCTGGCCCA GTTCAAGATG TAGTTGTGTC TGGAGAAACT
    GCAGATGACT CAGTACAAGA TTTAGTTGAG ACTGGAGAAG TTTTAGATGA TTCCGTACAA
    GATGTACTTG AGCATGAAGT AATTTCATTT GGCCCAGTAC AAGATGTAGT TGTATCTGGA
    AAAACTTCAA ATGACTCAGT ACAAGATGTA GTTGAGAGTG CAAAAGATTC AGATGCCCCA
    ATACAAGATG TATTTGAGCA TAAAGACATT TCAGTTGACT CTGTACAAGA TGTAGTTGTG
    TCTGGAGAAA CTGCAGATGA CTCTATTCAA GATTTATTTA AGCATGAAGG CACTTCGTTC
    GACACAGTAC AAGATGAGGT TGAGTCTAGA GAAACTTCAG ATGACTCAGT ACAAAATGTA
    GTTGAGCATG AAGACTCTTC AGATGACGCA GTTCAAGATG TAGTCGAGTC TGGAGACACT
    TCAGATGACT CTGTACAAGA TATATTTGAG TCTGCAGAAA CTTCAGATGA CTCTGTACAA
    GATGTAGCTG AGACTGGAAA AGAATCAGAT GACTTAGTAC AAGATATATT TGAGAATAAA
    GACATTTCAG TTGACTCAGT ACAAGATGTA GTTGTATCTG GAGAGACTTC AGATGACTCT
    ATTCAAGGTT TACTTGAGCA TGAAGGCACT TCAGTCGACA TAGTACAAGA TGAGGTTGAG
    TTTAGAGAAA ATTCAGATGA CTCAGTACTA AATGTAGTCA AGCATGAAGA TACTTCAGAT
    GACTCAATAC AAGATGTAGT CGAGACAGGA GAAGTTTCAG ATAGCTCAGT ACAAGATGTA
    GCTGAGTCTG GAGAAACTTT AGATGACTCT GTTCAAGATT TTGTTAAAAC TGGAGAAATT
    TCCATTGACT CAGTACACAA TTTATTTGAG CCTGAAAAAA ACGTAGATGA CTCTGTACAA
    GATGTAGTTG AGACTGGAGA AGTTTCTGTT GATTCAGTAC AAGATGTAAT TGAGCTTGGA
    GACACTTTAG ATGACTCTGC ACAAGGAATA GTTGAACCTG AAGAAATGGC AGACAACTTT
    ATAAAGGAAA GCACTGTAAA GCCAAGACCT GACATCAAAG GGCTTATTCT ACCAACAGAG
    ACAGTGAACT ATGAAAGTGC CACTATGAAA GTTCCTGCTC CAGTTAACCC CAGCATAACA
    GAATTTAAGG TTTCATTTGA CATCTTCCCT TTCGACGACC CAACTGATGA TGAAGATAAT
    GGCAGTGGGT TTGCTCATGG AACTGACTTA GCAAGTTTAG CCTTGCCTGC CAGTCCTGGA
    AGGGCATTAA TAGTGTTTTT TAGCCTTAGG GTCACCAACA TGAAGTTTTC CGAAGATCTC
    TTCAACAAGA GCTCTACTGA ATACAAGGCA CTTGAGCAGC GATTTCTGGA ACTGCTCGTA
    CCGTATCTAC AGTCCAACCT CAGCAATTTC CAGAATTTAG AGATTTTGAA CTTCAGGAAT
    GGTAGCATTG TGGTGAACAG CAGGATGAAG TTTGCCAAGC CAGTTCCCCA TGGTGTGACA
    ACAGCAGTGT ACCTAATCTT GGAGGACTTC TGCAACACTG CCTACCAAAC TATGAATCTT
    GCAATAGACA AGTATTCATT GGATGTCGAA TCAGGTGACC AGGCAGATCC TTGTAAGTTT
    CAGGCATGTA ACGAGTTCTC CAAGTGTTTG GTGAACCGCT GGTCAGGTGA GGCCGAGTGT
    GTTTGTAACG CTGGTTATTT CAGCGTTGAC GGACTGCCAT GCCAGAGCAT CTGCGACATC
    CAAGAAGACT TCTGCCTAAA TGACGGGAAA TGTGACATAA TACCTGGCAA GGGCGCCATC
    TGCAGGTGTC GCGTTGGTGA AAACTGGTGG TATCGAGGAG AACACTGTGA GGAATATGTG
    TCAGAGCCGC TGGTGGTCGG CATTGCCATT GCGTCCGTTG CTGGATTTCT CCTGGTGGCG
    TCGGGTGTCA TCTTTTTCCT GGCCCGAACT CTGAGAGACC AGTATGACAC AGATGATTCA
    GAAGACCCTT TACGATATGC AGAGAACTTG CCATCTCTAG AAAGAGCCAC CAAGTACAAC
    CCGATGTTTG AAAGTGAAGT GACTGCAGGC CATAATCAGT ATTATCGGCG TTACCCAGAA
    GCTCCGGCAT TCAGCACGGG CAGCACCGAG ATCTCCACTG ACTTCAGCAG CGAAGAGATT
    CGTCACATCT ATCAGAACAG TGAACTTACT AAAGAGGAAA TCCAGGACCG TATTCGTATT
    ATAGAGCTTT ACGCCAAGGA CCGCCAGTTT GCAGATTTTG TCCGACAGCA CCAAGTGGCG
    CTTGACGCTA GACGAGAGAG CTCTTCCAAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009294723.1
    CDS212..8644
    Translation

    Target ORF information:

    RefSeq Version XM_009296448.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio interphotoreceptor matrix proteoglycan 2b (impg2b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296448.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGCCTGATT TACCTTTTAA ATGCATTTGG TGGGGTTTGA CACTATTGTT GTTGACTGGA 
    TACTTTTCTA TAGATGCAGA TACTGGAACT CATGAGGATT ACTTGGCAAA TGGAAATACA
    ATGCTTTTGG ACACTAATGA AGTTTTGAAT CACTTATTGT TGTTGGACAA AGGTAAAACT
    TTGAATTACA TGTCTGCTGA AGGGAAAACA GCTCTATCTA GACATAAAAG GGACCTCCTG
    TTCCCCAATG GAGTTAAACT GTGCTCACAT GAGACGGTGC AACAGGCAGT GCAGAATCAT
    CTCAACTACT TTCACCTGCG AGTGTGCCAG GAGACAATTT GGGAGGCTTA TAAAATCTTC
    TGGGATCGCT TACCTGAGAA AGAAGAGTAC CAGATCTGGA TGAAAAAATG TCAAAATGGT
    AGCATTAGCG TGTTTGACAT CGGCCGAAGC TTTAGTCAGT CAGCTGAGCA CCTTGCTTTG
    ATTTCAAGTA GGGTAGCATC ATCCACAATA ACATCAACAA GGCATCAGAC GACGACTACT
    TCGCCAACAC TAGCAAAAGC TGAACAAGTT GGTATTTTGA TTCCTGAGGC AGTGCCAGGT
    TCACAAGTTG TCACACCGCA GACCTTTGCA GATGATATCG TAATCACAAC TTTGCCTACA
    AGGCTTCAAG TGACACCTGG AATTACCCCA AAGCCTGATC TTGTCACAGC CTTTAAGGAC
    ACAGTAAAAC CTATTTCTGA AACTGAACCT GAGGTCATAT TGGGTACTAC TTCTGAGAGT
    CCTGTTAAAG CATTGGATAC CATTACCATT AAACATGAAG AGGTTGTTGT AGTCTCAGAA
    TCTAAATTAT TGATTACTGA GAGGACTTCG GAGGACTCTA CTTCAAGGAA ATATGATTAT
    GAGTTTCCCA CGACCTTTAA GGACTCGTTA GTAATAAACA CTCCTATAGT ACTAACAACT
    GAATTTTCTG AACAAATGTA TTCAGAAATA GGACAAGGTT TTTCTGATGG TACAGTTAAA
    ACAGTAGCTG TTACTATTCA AAAGGAACTT GACATTGTCT CAAAATCTAC ATTATTAACC
    ACTGACAAAA CTCCAGAAGT TTCTACTTCA AAAAAATCTG AGCATGATAA TCCCACTAAC
    TTTGTAGATT CATTGGGAAT AAGCACCAAT ACTCCTAGAG TGACCACAAG TGAACTCAAA
    GAAGATCTTC ATTCAGAATT AGAACAGGTG GCTGGGCAGG ATGTTATTCA CAGTCCTGTT
    AAAACAACAC ATGTTACAAT TAAGCATGAA GTGTTGGACA CAAATACTAC ATTAATGACA
    ACTGACAGAA CTCCATATTC TACTTCAGCA AAATATGAAC GTGTTATACC AACATCCTTT
    GAGGACTCAC TAGTCACAAG TTCATCTACT GAACAGGATA TGACCAATGT TCCTATTAAG
    ACGGCATATG CCACAATTTC ACATGGAGAG TCTGAAATTG TCACAGAGTC GACAATAACA
    ACCACTAACA GAAATCTTGA AGATTCTTCT TTAGCAAAAT ATGATTATGG GATTCCTCAA
    GGAATTGATA CTTCTTCGGG AGTAATTAAT GAATCTTCTA CAAAAGAACA TAATTCAGTA
    TCTAAATTGA ATATTGGTCA GACTACTACT GAAGGTCCCC AAACAACTGA TGCAGTTCAG
    TATGTAGAAT CTGACATTGT GTCAGAGGAA ACGTTGAAAT CCACTGGCAG AACTGCAGTT
    AGCTCAAACT CTACAAAAAT TGAGGCCGAT CTTTCCACAA CTCCTGAGGA CCTGACAGGA
    GTAAGTACCA ACACTCCTGA AATAGTCACT AGAGATTCCA TTACAATACT TATCCCAGAA
    ACCGAGGGCA TTACTAAAAA TCCTGTTAAA TCTGCACCTA TTACCATTCA GCATGTGGAT
    GTTGATGATG TTTTAGCAGC TACAGTGATG TCCACTCATA AACCTCCCAA AGTCTCACCT
    ACAGAAATAT CCCCCGATCA GAATCTCTTA CCAGAGGAAT CAAAGCTGCC AACTCCTACA
    AAACTAGCAG AAGTTTTGTC TGCCACAGAA GAGTTGCCAA AGGATCAGAC AGACAAGGAT
    GTTGCTAAAA TTGTATATGT GTCTACTGTG CCAGAACTGG TGTCACCAAC TATTCCCGCA
    TATACGTCAA CTGACACTAC TAAGGAAAAC CTACATGTGT TAACAGGGGG GGCAGTTGTT
    ACATCACTTG ATAAAGATGA GGAAGAACAT ACACCCCACA CAAAAGAGAG CAAAATTACA
    CAGGTGACAA CAGGAGCAGA ATTACCAGGT ACCATAACAG AGAGTACTCA TGGAATGTGG
    AGGGAAACCC AAGAGCCAAT AAATGTCACA CCTGATGCTG AAGACAATCA TTTGGGCAAA
    GAAGGGTACA TAATAACAGA GATCCCAGAA GTTGCTGAAG TACCAGCTGG AGTGACACCC
    AAAATGATTG TGGAAACTAC AACAGCCACT GAATCTGTGT TTACTGTGAA AGACACATCT
    ATAATTGTAG ACAAAACTGG CATTGTGAAA CCTGTCGTGA CTACGACAGA GGCAGCTGAA
    CTTACTGAGG TTGGATTCGA AGATTTAGGC ACAGACAAAG TAACAATTCT TGAAAACACA
    CTTGAAGATT CTGTAGGACT AACAAATGAA CCTTTTGCTG TATCATGGGG TGTAACTGAA
    GCCACTATTT TAGAGGAATC ATATGTGGAA AGTACTGAAA GTATTTCAGG TAAAGAGAAG
    TCCACTGAAA CTATTAAGAT CACAGGTGAA GCCACAACTG ATGACATTGG TACTTCTGAA
    ACTGACATTG TAGTGCCTGT AACCGATCGC ATAGAAGATA ATGTAAATGT ATCACCTAAA
    AATGATGATG CTAAGGAAAC AAACATTTTT GAAGACACAA CACCAGATCT TAGTGTTACT
    AATTCTGAAA AAGACATACT GACAACTACC ATACATAAAG ACATGCATGA AGTTACAGAA
    CCATTAAATA TTCCAGAGGT CACACATAAG GTTGTGATAG AGATCACAGA TGATGTACGT
    GAGACCAGAG GAGATTCCTT ACCCGAGACA CCAGAAGTCA CAACAGAGAC ACCTAAGGTT
    GCAACAGAAG CTGATGTCAA AACAACAACA AATGTTATAT CTAAAGCACC TGAAGTTATA
    CTGAAGACAA CTGTAACCAA GTCAGATACA CCTGATGTCA CAACTCAGAC ACCTTTGTTA
    AAGCTTGAAG ATACAGAGAG GGTTGAAATA GTACCTGAAA CTCCTGACAT ACAAGTGGTT
    CAAACAGAAC ACTCAGAAAA TGATATCCCA ACTCCTGTCT TGAAAGTGGA GGATTTCACA
    GTTGCGCTGA ATGACTTGAC AACTCCTATT CAAAAGACTT CTTCAACCAC ATTACAACAA
    GACATTTCTA ATGATATTTT AGAAGAAAAC AATGTGATGG GAAATGAAAT TGATGATATT
    GTGGTTCGAC CAGTACGCCC TGTCGGCGAT CATATTTTGG AGCTAAGTAT TAAGCTAAGA
    GGGGAAAGCT ATGACGATGC TCTGAGGGAT CCATCAAGTA ACTACTATCA ACATCTGTCT
    ACACTTTTTG TTGAAAAGAT TGAAGAAGTT TTCGAAAGGC TTCCAGGTTT CAAGAAAATA
    TATGTTCTTG AATTCAGGCC ACAGAAGGAC ATTGAAGGAG GTCTGGCAGT GGTGGTGCAC
    TATGCTGTTG TTCTTGAGGG TGATGGGTCA GGAGTAAGTA ATGAGACCAT GGATTACATA
    AACCTGCATT CCAACATGGT TGAGAACTCC TTCACAGATC CTGAGGAGCT GCCCACTGTG
    GTCTACACCA TCACTGACTT CCGAAACTAC ATCTCCGAAG CCCTTCACAA GGATAACTTT
    ATCAGAAACG CTACTCTGAA TGTGGACATT GATTCTTTGC AACTTGAAAA TGTGGAGACA
    TTGCTGCCTT CCAAACCAAC CAGCAGACCG TTTGACTCCA GTGACATGAT GGACAACGTT
    CTTGCTTCCG AGAAACCACC AGATGTTCCT GGGCAAGAAT TATCCAACAA TGACATCTTC
    ATTACAAATT ATGACATCTT TGATCCTGAT CCATGGACAG GTGGACAAAG TGAAGACAGA
    AATGCAAATG ATGTAATCAT CTTTGAGGAA AGTCCCACTC AGTCTCCTGT TGAAGTGTCT
    TTGAAGAACT TGGACATTGA AGTTACTTCA AAATCTGAAA CTTCAAGCAG TGCCCCTGTT
    CCTGATGAAG CTGTTATTGA AGAGGAGGGA TTTTTACAGA CTGCAACTAC AGTCAGTCCT
    ATTATAGACA CAATAACTGA ATTTACTTTA CCTACAGAAT CACCTCACAA GGTAGAAGAG
    ACACCAGTTT CTCCTGTAGA ACCTCCTGAG GTTGAGGAAA CTACCCTGGG TGATTTAACA
    GACCTTGGCT CAGGCTCAGG ATTCTCTGGA GATCACATAG GCCCTGACAT CTGGCCATGG
    TCATCAGAAA CATCACAAGA AGTCTTGAAG GAAGATACCA ATGATGAGAT ACACCAACTT
    CAGGAAAATC AGGAATCTAT AATAAAACAG CTTGAGCCTT TACATCAAGA TGTGACTCCT
    GAGGAGCCAT TCTTGGATAG GGTTCTTGTG ACCCAAGACA TTCGTACCAA TCCTCATTAC
    ACAACAACTG ACCAGGCCCC TGTATTTTGG ACAATGGAGA CTTTGACTGT TGAGCTTTCA
    ATGCAGACTC AAGTAGCCCC TGAACATTAT GATGACTATT TTCCAGATGC ATCAATCACT
    ATGGCTACAC TTGTAACCAA TCAACCATTG TTACATGTTC ACAATCCATT AGAAACAAAA
    GATCTGGATG CATCACAAAG TCCAGGAACA ATAAGTTCAA CTGCTGATAA GGACTATACA
    GAACGACTTG TTACACCCAC TGTGATCCAA CCAAGTGAAA CAGTAGCTGT GGATAAGCAT
    ACTACAACAG ACAACAAACA GAATCTGGAA ACTATGGGTA CATTATCTGC AACACTGGGC
    TCCACAATGA AGTCCACTGA AATCTCTTCA ACTACCCAAT TACCTGTGAT CATCAACAAT
    AATACAGTTT CAGTGGAAGG TCCTGCCAAT TCTTCAGAAG CTGTTCAGCA TTCTGTAAAT
    GAAGGAATCA CAGAGTTACC TGCTTTGGTC TGGCCAGATA TTGAAGGATC AGATGAAGAA
    TTCAAAGTTT TAGATGAGGA CATGGAACTC GTCAAGTTTG TGCCTACAAA AAGCCCAGTG
    ACTGAACTCT TAGAAGAAGA TTTAGTTGGT GATGAAATAT TGGTTGCAAC AACAACAACA
    ACAACAACTC TATCCTCAAC AACAGTAGCG ACTGTGGTAA CAAGTTTAGG TCATTCAGCC
    TCCCTGACTC CTGAAAAGGA CTCACCTTTC ACTTGGATAT CACATTCATC AATAGATGAG
    GATGAACCTG TGCAAGAGTC AACCACAGAA CCTCTACCCA CCCAATCACC CACTGCTTCT
    GTTCTTCCAA AGACAACTAC ACAGACATTG ATGCACACAT ACATAGCAGC TGTTGCAGGG
    GAGCCTGTTT TATCAGATAA CATGGATAAT AAGGACTCAA CCACAATGTC CACCCTTTTA
    GATGTTTCAA AACATTTAAA GACCCTTAGT TCCACCTCTG TACAATCATT TTCAGAACCA
    ACATTTACAC CTACTGTCCA CATAATTAAG CATGGAGAAA CTTCAGATGA CTCAGTACAA
    GATGTAGTTG AGACTGGAGA AGTTTTAGAT GACTCTGTAC AAGATGTACT TGAGCATGAA
    GACATTTCAT ATGGCTCTGT ACAAGATGTA GTTGTATCTG GAGAAACTTC AAATGACTCA
    GTACAAGATG TAGTTGAGAC TGGAGAAGTT TTAGATGATT CTGTACAAGA TGTACTTGAG
    CATGAGGACA TTTTATCTGG CCCAGTTCAA GATGTAGTTG TGTCTGGAGA AACTGCAGAA
    GACTCAGTAC AAGATTTAGT TGAGACTAGA GAAGTTTTAG ATGATTCTGT ACAAGATGTA
    CTTGAGCATG AGGACATTTT ATCTGGCCCA GTTCAAGATG TAGTTGTGTC TGGAGAAACT
    GCAGATGACT CAGTACAAGA TTTAGTTGAG ACTGGAGAAG TTTTAGATGA TTCCGTACAA
    GATGTACTTG AGCATGAAGT AATTTCATTT GGCCCAGTAC AAGATGTAGT TGTATCTGGA
    AAAACTTCAA ATGACTCAGT ACAAGATGTA GTTGAGAGTG CAAAAGATTC AGATGCCCCA
    ATACAAGATG TATTTGAGCA TAAAGACATT TCAGTTGACT CTGTACAAGA TGTAGTTGTG
    TCTGGAGAAA CTGCAGATGA CTCTATTCAA GATTTATTTA AGCATGAAGG CACTTCGTTC
    GACACAGTAC AAGATGAGGT TGAGTCTAGA GAAACTTCAG ATGACTCAGT ACAAAATGTA
    GTTGAGCATG AAGACTCTTC AGATGACGCA GTTCAAGATG TAGTCGAGTC TGGAGACACT
    TCAGATGACT CTGTACAAGA TATATTTGAG TCTGCAGAAA CTTCAGATGA CTCTGTACAA
    GATGTAGCTG AGACTGGAAA AGAATCAGAT GACTTAGTAC AAGATATATT TGAGAATAAA
    GACATTTCAG TTGACTCAGT ACAAGATGTA GTTGTATCTG GAGAGACTTC AGATGACTCT
    ATTCAAGGTT TACTTGAGCA TGAAGGCACT TCAGTCGACA TAGTACAAGA TGAGGTTGAG
    TTTAGAGAAA ATTCAGATGA CTCAGTACTA AATGTAGTCA AGCATGAAGA TACTTCAGAT
    GACTCAATAC AAGATGTAGT CGAGACAGGA GAAGTTTCAG ATAGCTCAGT ACAAGATGTA
    GCTGAGTCTG GAGAAACTTT AGATGACTCT GTTCAAGATT TTGTTAAAAC TGGAGAAATT
    TCCATTGACT CAGTACACAA TTTATTTGAG CCTGAAAAAA ACGTAGATGA CTCTGTACAA
    GATGTAGTTG AGACTGGAGA AGTTTCTGTT GATTCAGTAC AAGATGTAAT TGAGCTTGGA
    GACACTTTAG ATGACTCTGC ACAAGGAATA GTTGAACCTG AAGAAATGGC AGACAACTTT
    ATAAAGGAAA GCACTGTAAA GCCAAGACCT GACATCAAAG GGCTTATTCT ACCAACAGAG
    ACAGTGAACT ATGAAAGTGC CACTATGAAA GTTCCTGCTC CAGTTAACCC CAGCATAACA
    GAATTTAAGG TTTCATTTGA CATCTTCCCT TTCGACGACC CAACTGATGA TGAAGATAAT
    GGCAGTGGGT TTGCTCATGG AACTGACTTA GCAAGTTTAG CCTTGCCTGC CAGTCCTGGA
    AGGGCATTAA TAGTGTTTTT TAGCCTTAGG GTCACCAACA TGAAGTTTTC CGAAGATCTC
    TTCAACAAGA GCTCTACTGA ATACAAGGCA CTTGAGCAGC GATTTCTGGA ACTGCTCGTA
    CCGTATCTAC AGTCCAACCT CAGCAATTTC CAGAATTTAG AGATTTTGAA CTTCAGGAAT
    GGTAGCATTG TGGTGAACAG CAGGATGAAG TTTGCCAAGC CAGTTCCCCA TGGTGTGACA
    ACAGCAGTGT ACCTAATCTT GGAGGACTTC TGCAACACTG CCTACCAAAC TATGAATCTT
    GCAATAGACA AGTATTCATT GGATGTCGAA TCAGGTGACC AGGCAGATCC TTGTAAGTTT
    CAGGCATGTA ACGAGTTCTC CAAGTGTTTG GTGAACCGCT GGTCAGGTGA GGCCGAGTGT
    GTTTGTAACG CTGGTTATTT CAGCGTTGAC GGACTGCCAT GCCAGAGCAT CTGCGACATC
    CAAGAAGACT TCTGCCTAAA TGACGGGAAA TGTGACATAA TACCTGGCAA GGGCGCCATC
    TGCAGGTGTC GCGTTGGTGA AAACTGGTGG TATCGAGGAG AACACTGTGA GGAATATGTG
    TCAGAGCCGC TGGTGGTCGG CATTGCCATT GCGTCCGTTG CTGGATTTCT CCTGGTGGCG
    TCGGGTGTCA TCTTTTTCCT GGCCCGAACT CTGAGAGACC AGTATGACAC AGATGATTCA
    GAAGACCCTT TACGATATGC AGAGAACTTG CCATCTCTAG AAAGAGCCAC CAAGTACAAC
    CCGATGTTTG AAAGTGAAGT GACTGCAGGC CATAATCAGT ATTATCGGCG TTACCCAGAA
    GCTCCGGCAT TCAGCACGGG CAGCACCGAG ATCTCCACTG ACTTCAGCAG CGAAGAGATT
    CGTCACATCT ATCAGAACAG TGAACTTACT AAAGAGGAAA TCCAGGACCG TATTCGTATT
    ATAGAGCTTT ACGCCAAGGA CCGCCAGTTT GCAGATTTTG TCCGACAGCA CCAAGTGGCG
    CTTGACGCTA GACGAGAGAG CTCTTCCAAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45891
    Clone ID Related Accession (Same CDS sequence) XM_021474485.1 , XM_009296448.2 , XM_009296448.3 , XM_021473521.1
    Accession Version XM_009296448.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin homolog isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296448.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGCCTGATT TACCTTTTAA ATGCATTTGG TGGGGTTTGA CACTATTGTT GTTGACTGGA 
    TACTTTTCTA TAGATGCAGA TACTGGAACT CATGAGGATT ACTTGGCAAA TGGAAATACA
    ATGCTTTTGG ACACTAATGA AGTTTTGAAT CACTTATTGT TGTTGGACAA AGGTAAAACT
    TTGAATTACA TGTCTGCTGA AGGGAAAACA GCTCTATCTA GACATAAAAG GGACCTCCTG
    TTCCCCAATG GAGTTAAACT GTGCTCACAT GAGACGGTGC AACAGGCAGT GCAGAATCAT
    CTCAACTACT TTCACCTGCG AGTGTGCCAG GAGACAATTT GGGAGGCTTA TAAAATCTTC
    TGGGATCGCT TACCTGAGAA AGAAGAGTAC CAGATCTGGA TGAAAAAATG TCAAAATGGT
    AGCATTAGCG TGTTTGACAT CGGCCGAAGC TTTAGTCAGT CAGCTGAGCA CCTTGCTTTG
    ATTTCAAGTA GGGTAGCATC ATCCACAATA ACATCAACAA GGCATCAGAC GACGACTACT
    TCGCCAACAC TAGCAAAAGC TGAACAAGTT GGTATTTTGA TTCCTGAGGC AGTGCCAGGT
    TCACAAGTTG TCACACCGCA GACCTTTGCA GATGATATCG TAATCACAAC TTTGCCTACA
    AGGCTTCAAG TGACACCTGG AATTACCCCA AAGCCTGATC TTGTCACAGC CTTTAAGGAC
    ACAGTAAAAC CTATTTCTGA AACTGAACCT GAGGTCATAT TGGGTACTAC TTCTGAGAGT
    CCTGTTAAAG CATTGGATAC CATTACCATT AAACATGAAG AGGTTGTTGT AGTCTCAGAA
    TCTAAATTAT TGATTACTGA GAGGACTTCG GAGGACTCTA CTTCAAGGAA ATATGATTAT
    GAGTTTCCCA CGACCTTTAA GGACTCGTTA GTAATAAACA CTCCTATAGT ACTAACAACT
    GAATTTTCTG AACAAATGTA TTCAGAAATA GGACAAGGTT TTTCTGATGG TACAGTTAAA
    ACAGTAGCTG TTACTATTCA AAAGGAACTT GACATTGTCT CAAAATCTAC ATTATTAACC
    ACTGACAAAA CTCCAGAAGT TTCTACTTCA AAAAAATCTG AGCATGATAA TCCCACTAAC
    TTTGTAGATT CATTGGGAAT AAGCACCAAT ACTCCTAGAG TGACCACAAG TGAACTCAAA
    GAAGATCTTC ATTCAGAATT AGAACAGGTG GCTGGGCAGG ATGTTATTCA CAGTCCTGTT
    AAAACAACAC ATGTTACAAT TAAGCATGAA GTGTTGGACA CAAATACTAC ATTAATGACA
    ACTGACAGAA CTCCATATTC TACTTCAGCA AAATATGAAC GTGTTATACC AACATCCTTT
    GAGGACTCAC TAGTCACAAG TTCATCTACT GAACAGGATA TGACCAATGT TCCTATTAAG
    ACGGCATATG CCACAATTTC ACATGGAGAG TCTGAAATTG TCACAGAGTC GACAATAACA
    ACCACTAACA GAAATCTTGA AGATTCTTCT TTAGCAAAAT ATGATTATGG GATTCCTCAA
    GGAATTGATA CTTCTTCGGG AGTAATTAAT GAATCTTCTA CAAAAGAACA TAATTCAGTA
    TCTAAATTGA ATATTGGTCA GACTACTACT GAAGGTCCCC AAACAACTGA TGCAGTTCAG
    TATGTAGAAT CTGACATTGT GTCAGAGGAA ACGTTGAAAT CCACTGGCAG AACTGCAGTT
    AGCTCAAACT CTACAAAAAT TGAGGCCGAT CTTTCCACAA CTCCTGAGGA CCTGACAGGA
    GTAAGTACCA ACACTCCTGA AATAGTCACT AGAGATTCCA TTACAATACT TATCCCAGAA
    ACCGAGGGCA TTACTAAAAA TCCTGTTAAA TCTGCACCTA TTACCATTCA GCATGTGGAT
    GTTGATGATG TTTTAGCAGC TACAGTGATG TCCACTCATA AACCTCCCAA AGTCTCACCT
    ACAGAAATAT CCCCCGATCA GAATCTCTTA CCAGAGGAAT CAAAGCTGCC AACTCCTACA
    AAACTAGCAG AAGTTTTGTC TGCCACAGAA GAGTTGCCAA AGGATCAGAC AGACAAGGAT
    GTTGCTAAAA TTGTATATGT GTCTACTGTG CCAGAACTGG TGTCACCAAC TATTCCCGCA
    TATACGTCAA CTGACACTAC TAAGGAAAAC CTACATGTGT TAACAGGGGG GGCAGTTGTT
    ACATCACTTG ATAAAGATGA GGAAGAACAT ACACCCCACA CAAAAGAGAG CAAAATTACA
    CAGGTGACAA CAGGAGCAGA ATTACCAGGT ACCATAACAG AGAGTACTCA TGGAATGTGG
    AGGGAAACCC AAGAGCCAAT AAATGTCACA CCTGATGCTG AAGACAATCA TTTGGGCAAA
    GAAGGGTACA TAATAACAGA GATCCCAGAA GTTGCTGAAG TACCAGCTGG AGTGACACCC
    AAAATGATTG TGGAAACTAC AACAGCCACT GAATCTGTGT TTACTGTGAA AGACACATCT
    ATAATTGTAG ACAAAACTGG CATTGTGAAA CCTGTCGTGA CTACGACAGA GGCAGCTGAA
    CTTACTGAGG TTGGATTCGA AGATTTAGGC ACAGACAAAG TAACAATTCT TGAAAACACA
    CTTGAAGATT CTGTAGGACT AACAAATGAA CCTTTTGCTG TATCATGGGG TGTAACTGAA
    GCCACTATTT TAGAGGAATC ATATGTGGAA AGTACTGAAA GTATTTCAGG TAAAGAGAAG
    TCCACTGAAA CTATTAAGAT CACAGGTGAA GCCACAACTG ATGACATTGG TACTTCTGAA
    ACTGACATTG TAGTGCCTGT AACCGATCGC ATAGAAGATA ATGTAAATGT ATCACCTAAA
    AATGATGATG CTAAGGAAAC AAACATTTTT GAAGACACAA CACCAGATCT TAGTGTTACT
    AATTCTGAAA AAGACATACT GACAACTACC ATACATAAAG ACATGCATGA AGTTACAGAA
    CCATTAAATA TTCCAGAGGT CACACATAAG GTTGTGATAG AGATCACAGA TGATGTACGT
    GAGACCAGAG GAGATTCCTT ACCCGAGACA CCAGAAGTCA CAACAGAGAC ACCTAAGGTT
    GCAACAGAAG CTGATGTCAA AACAACAACA AATGTTATAT CTAAAGCACC TGAAGTTATA
    CTGAAGACAA CTGTAACCAA GTCAGATACA CCTGATGTCA CAACTCAGAC ACCTTTGTTA
    AAGCTTGAAG ATACAGAGAG GGTTGAAATA GTACCTGAAA CTCCTGACAT ACAAGTGGTT
    CAAACAGAAC ACTCAGAAAA TGATATCCCA ACTCCTGTCT TGAAAGTGGA GGATTTCACA
    GTTGCGCTGA ATGACTTGAC AACTCCTATT CAAAAGACTT CTTCAACCAC ATTACAACAA
    GACATTTCTA ATGATATTTT AGAAGAAAAC AATGTGATGG GAAATGAAAT TGATGATATT
    GTGGTTCGAC CAGTACGCCC TGTCGGCGAT CATATTTTGG AGCTAAGTAT TAAGCTAAGA
    GGGGAAAGCT ATGACGATGC TCTGAGGGAT CCATCAAGTA ACTACTATCA ACATCTGTCT
    ACACTTTTTG TTGAAAAGAT TGAAGAAGTT TTCGAAAGGC TTCCAGGTTT CAAGAAAATA
    TATGTTCTTG AATTCAGGCC ACAGAAGGAC ATTGAAGGAG GTCTGGCAGT GGTGGTGCAC
    TATGCTGTTG TTCTTGAGGG TGATGGGTCA GGAGTAAGTA ATGAGACCAT GGATTACATA
    AACCTGCATT CCAACATGGT TGAGAACTCC TTCACAGATC CTGAGGAGCT GCCCACTGTG
    GTCTACACCA TCACTGACTT CCGAAACTAC ATCTCCGAAG CCCTTCACAA GGATAACTTT
    ATCAGAAACG CTACTCTGAA TGTGGACATT GATTCTTTGC AACTTGAAAA TGTGGAGACA
    TTGCTGCCTT CCAAACCAAC CAGCAGACCG TTTGACTCCA GTGACATGAT GGACAACGTT
    CTTGCTTCCG AGAAACCACC AGATGTTCCT GGGCAAGAAT TATCCAACAA TGACATCTTC
    ATTACAAATT ATGACATCTT TGATCCTGAT CCATGGACAG GTGGACAAAG TGAAGACAGA
    AATGCAAATG ATGTAATCAT CTTTGAGGAA AGTCCCACTC AGTCTCCTGT TGAAGTGTCT
    TTGAAGAACT TGGACATTGA AGTTACTTCA AAATCTGAAA CTTCAAGCAG TGCCCCTGTT
    CCTGATGAAG CTGTTATTGA AGAGGAGGGA TTTTTACAGA CTGCAACTAC AGTCAGTCCT
    ATTATAGACA CAATAACTGA ATTTACTTTA CCTACAGAAT CACCTCACAA GGTAGAAGAG
    ACACCAGTTT CTCCTGTAGA ACCTCCTGAG GTTGAGGAAA CTACCCTGGG TGATTTAACA
    GACCTTGGCT CAGGCTCAGG ATTCTCTGGA GATCACATAG GCCCTGACAT CTGGCCATGG
    TCATCAGAAA CATCACAAGA AGTCTTGAAG GAAGATACCA ATGATGAGAT ACACCAACTT
    CAGGAAAATC AGGAATCTAT AATAAAACAG CTTGAGCCTT TACATCAAGA TGTGACTCCT
    GAGGAGCCAT TCTTGGATAG GGTTCTTGTG ACCCAAGACA TTCGTACCAA TCCTCATTAC
    ACAACAACTG ACCAGGCCCC TGTATTTTGG ACAATGGAGA CTTTGACTGT TGAGCTTTCA
    ATGCAGACTC AAGTAGCCCC TGAACATTAT GATGACTATT TTCCAGATGC ATCAATCACT
    ATGGCTACAC TTGTAACCAA TCAACCATTG TTACATGTTC ACAATCCATT AGAAACAAAA
    GATCTGGATG CATCACAAAG TCCAGGAACA ATAAGTTCAA CTGCTGATAA GGACTATACA
    GAACGACTTG TTACACCCAC TGTGATCCAA CCAAGTGAAA CAGTAGCTGT GGATAAGCAT
    ACTACAACAG ACAACAAACA GAATCTGGAA ACTATGGGTA CATTATCTGC AACACTGGGC
    TCCACAATGA AGTCCACTGA AATCTCTTCA ACTACCCAAT TACCTGTGAT CATCAACAAT
    AATACAGTTT CAGTGGAAGG TCCTGCCAAT TCTTCAGAAG CTGTTCAGCA TTCTGTAAAT
    GAAGGAATCA CAGAGTTACC TGCTTTGGTC TGGCCAGATA TTGAAGGATC AGATGAAGAA
    TTCAAAGTTT TAGATGAGGA CATGGAACTC GTCAAGTTTG TGCCTACAAA AAGCCCAGTG
    ACTGAACTCT TAGAAGAAGA TTTAGTTGGT GATGAAATAT TGGTTGCAAC AACAACAACA
    ACAACAACTC TATCCTCAAC AACAGTAGCG ACTGTGGTAA CAAGTTTAGG TCATTCAGCC
    TCCCTGACTC CTGAAAAGGA CTCACCTTTC ACTTGGATAT CACATTCATC AATAGATGAG
    GATGAACCTG TGCAAGAGTC AACCACAGAA CCTCTACCCA CCCAATCACC CACTGCTTCT
    GTTCTTCCAA AGACAACTAC ACAGACATTG ATGCACACAT ACATAGCAGC TGTTGCAGGG
    GAGCCTGTTT TATCAGATAA CATGGATAAT AAGGACTCAA CCACAATGTC CACCCTTTTA
    GATGTTTCAA AACATTTAAA GACCCTTAGT TCCACCTCTG TACAATCATT TTCAGAACCA
    ACATTTACAC CTACTGTCCA CATAATTAAG CATGGAGAAA CTTCAGATGA CTCAGTACAA
    GATGTAGTTG AGACTGGAGA AGTTTTAGAT GACTCTGTAC AAGATGTACT TGAGCATGAA
    GACATTTCAT ATGGCTCTGT ACAAGATGTA GTTGTATCTG GAGAAACTTC AAATGACTCA
    GTACAAGATG TAGTTGAGAC TGGAGAAGTT TTAGATGATT CTGTACAAGA TGTACTTGAG
    CATGAGGACA TTTTATCTGG CCCAGTTCAA GATGTAGTTG TGTCTGGAGA AACTGCAGAA
    GACTCAGTAC AAGATTTAGT TGAGACTAGA GAAGTTTTAG ATGATTCTGT ACAAGATGTA
    CTTGAGCATG AGGACATTTT ATCTGGCCCA GTTCAAGATG TAGTTGTGTC TGGAGAAACT
    GCAGATGACT CAGTACAAGA TTTAGTTGAG ACTGGAGAAG TTTTAGATGA TTCCGTACAA
    GATGTACTTG AGCATGAAGT AATTTCATTT GGCCCAGTAC AAGATGTAGT TGTATCTGGA
    AAAACTTCAA ATGACTCAGT ACAAGATGTA GTTGAGAGTG CAAAAGATTC AGATGCCCCA
    ATACAAGATG TATTTGAGCA TAAAGACATT TCAGTTGACT CTGTACAAGA TGTAGTTGTG
    TCTGGAGAAA CTGCAGATGA CTCTATTCAA GATTTATTTA AGCATGAAGG CACTTCGTTC
    GACACAGTAC AAGATGAGGT TGAGTCTAGA GAAACTTCAG ATGACTCAGT ACAAAATGTA
    GTTGAGCATG AAGACTCTTC AGATGACGCA GTTCAAGATG TAGTCGAGTC TGGAGACACT
    TCAGATGACT CTGTACAAGA TATATTTGAG TCTGCAGAAA CTTCAGATGA CTCTGTACAA
    GATGTAGCTG AGACTGGAAA AGAATCAGAT GACTTAGTAC AAGATATATT TGAGAATAAA
    GACATTTCAG TTGACTCAGT ACAAGATGTA GTTGTATCTG GAGAGACTTC AGATGACTCT
    ATTCAAGGTT TACTTGAGCA TGAAGGCACT TCAGTCGACA TAGTACAAGA TGAGGTTGAG
    TTTAGAGAAA ATTCAGATGA CTCAGTACTA AATGTAGTCA AGCATGAAGA TACTTCAGAT
    GACTCAATAC AAGATGTAGT CGAGACAGGA GAAGTTTCAG ATAGCTCAGT ACAAGATGTA
    GCTGAGTCTG GAGAAACTTT AGATGACTCT GTTCAAGATT TTGTTAAAAC TGGAGAAATT
    TCCATTGACT CAGTACACAA TTTATTTGAG CCTGAAAAAA ACGTAGATGA CTCTGTACAA
    GATGTAGTTG AGACTGGAGA AGTTTCTGTT GATTCAGTAC AAGATGTAAT TGAGCTTGGA
    GACACTTTAG ATGACTCTGC ACAAGGAATA GTTGAACCTG AAGAAATGGC AGACAACTTT
    ATAAAGGAAA GCACTGTAAA GCCAAGACCT GACATCAAAG GGCTTATTCT ACCAACAGAG
    ACAGTGAACT ATGAAAGTGC CACTATGAAA GTTCCTGCTC CAGTTAACCC CAGCATAACA
    GAATTTAAGG TTTCATTTGA CATCTTCCCT TTCGACGACC CAACTGATGA TGAAGATAAT
    GGCAGTGGGT TTGCTCATGG AACTGACTTA GCAAGTTTAG CCTTGCCTGC CAGTCCTGGA
    AGGGCATTAA TAGTGTTTTT TAGCCTTAGG GTCACCAACA TGAAGTTTTC CGAAGATCTC
    TTCAACAAGA GCTCTACTGA ATACAAGGCA CTTGAGCAGC GATTTCTGGA ACTGCTCGTA
    CCGTATCTAC AGTCCAACCT CAGCAATTTC CAGAATTTAG AGATTTTGAA CTTCAGGAAT
    GGTAGCATTG TGGTGAACAG CAGGATGAAG TTTGCCAAGC CAGTTCCCCA TGGTGTGACA
    ACAGCAGTGT ACCTAATCTT GGAGGACTTC TGCAACACTG CCTACCAAAC TATGAATCTT
    GCAATAGACA AGTATTCATT GGATGTCGAA TCAGGTGACC AGGCAGATCC TTGTAAGTTT
    CAGGCATGTA ACGAGTTCTC CAAGTGTTTG GTGAACCGCT GGTCAGGTGA GGCCGAGTGT
    GTTTGTAACG CTGGTTATTT CAGCGTTGAC GGACTGCCAT GCCAGAGCAT CTGCGACATC
    CAAGAAGACT TCTGCCTAAA TGACGGGAAA TGTGACATAA TACCTGGCAA GGGCGCCATC
    TGCAGGTGTC GCGTTGGTGA AAACTGGTGG TATCGAGGAG AACACTGTGA GGAATATGTG
    TCAGAGCCGC TGGTGGTCGG CATTGCCATT GCGTCCGTTG CTGGATTTCT CCTGGTGGCG
    TCGGGTGTCA TCTTTTTCCT GGCCCGAACT CTGAGAGACC AGTATGACAC AGATGATTCA
    GAAGACCCTT TACGATATGC AGAGAACTTG CCATCTCTAG AAAGAGCCAC CAAGTACAAC
    CCGATGTTTG AAAGTGAAGT GACTGCAGGC CATAATCAGT ATTATCGGCG TTACCCAGAA
    GCTCCGGCAT TCAGCACGGG CAGCACCGAG ATCTCCACTG ACTTCAGCAG CGAAGAGATT
    CGTCACATCT ATCAGAACAG TGAACTTACT AAAGAGGAAA TCCAGGACCG TATTCGTATT
    ATAGAGCTTT ACGCCAAGGA CCGCCAGTTT GCAGATTTTG TCCGACAGCA CCAAGTGGCG
    CTTGACGCTA GACGAGAGAG CTCTTCCAAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009294723.1
    CDS233..8665
    Translation

    Target ORF information:

    RefSeq Version XM_009296448.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio interphotoreceptor matrix proteoglycan 2b (impg2b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296448.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGCCTGATT TACCTTTTAA ATGCATTTGG TGGGGTTTGA CACTATTGTT GTTGACTGGA 
    TACTTTTCTA TAGATGCAGA TACTGGAACT CATGAGGATT ACTTGGCAAA TGGAAATACA
    ATGCTTTTGG ACACTAATGA AGTTTTGAAT CACTTATTGT TGTTGGACAA AGGTAAAACT
    TTGAATTACA TGTCTGCTGA AGGGAAAACA GCTCTATCTA GACATAAAAG GGACCTCCTG
    TTCCCCAATG GAGTTAAACT GTGCTCACAT GAGACGGTGC AACAGGCAGT GCAGAATCAT
    CTCAACTACT TTCACCTGCG AGTGTGCCAG GAGACAATTT GGGAGGCTTA TAAAATCTTC
    TGGGATCGCT TACCTGAGAA AGAAGAGTAC CAGATCTGGA TGAAAAAATG TCAAAATGGT
    AGCATTAGCG TGTTTGACAT CGGCCGAAGC TTTAGTCAGT CAGCTGAGCA CCTTGCTTTG
    ATTTCAAGTA GGGTAGCATC ATCCACAATA ACATCAACAA GGCATCAGAC GACGACTACT
    TCGCCAACAC TAGCAAAAGC TGAACAAGTT GGTATTTTGA TTCCTGAGGC AGTGCCAGGT
    TCACAAGTTG TCACACCGCA GACCTTTGCA GATGATATCG TAATCACAAC TTTGCCTACA
    AGGCTTCAAG TGACACCTGG AATTACCCCA AAGCCTGATC TTGTCACAGC CTTTAAGGAC
    ACAGTAAAAC CTATTTCTGA AACTGAACCT GAGGTCATAT TGGGTACTAC TTCTGAGAGT
    CCTGTTAAAG CATTGGATAC CATTACCATT AAACATGAAG AGGTTGTTGT AGTCTCAGAA
    TCTAAATTAT TGATTACTGA GAGGACTTCG GAGGACTCTA CTTCAAGGAA ATATGATTAT
    GAGTTTCCCA CGACCTTTAA GGACTCGTTA GTAATAAACA CTCCTATAGT ACTAACAACT
    GAATTTTCTG AACAAATGTA TTCAGAAATA GGACAAGGTT TTTCTGATGG TACAGTTAAA
    ACAGTAGCTG TTACTATTCA AAAGGAACTT GACATTGTCT CAAAATCTAC ATTATTAACC
    ACTGACAAAA CTCCAGAAGT TTCTACTTCA AAAAAATCTG AGCATGATAA TCCCACTAAC
    TTTGTAGATT CATTGGGAAT AAGCACCAAT ACTCCTAGAG TGACCACAAG TGAACTCAAA
    GAAGATCTTC ATTCAGAATT AGAACAGGTG GCTGGGCAGG ATGTTATTCA CAGTCCTGTT
    AAAACAACAC ATGTTACAAT TAAGCATGAA GTGTTGGACA CAAATACTAC ATTAATGACA
    ACTGACAGAA CTCCATATTC TACTTCAGCA AAATATGAAC GTGTTATACC AACATCCTTT
    GAGGACTCAC TAGTCACAAG TTCATCTACT GAACAGGATA TGACCAATGT TCCTATTAAG
    ACGGCATATG CCACAATTTC ACATGGAGAG TCTGAAATTG TCACAGAGTC GACAATAACA
    ACCACTAACA GAAATCTTGA AGATTCTTCT TTAGCAAAAT ATGATTATGG GATTCCTCAA
    GGAATTGATA CTTCTTCGGG AGTAATTAAT GAATCTTCTA CAAAAGAACA TAATTCAGTA
    TCTAAATTGA ATATTGGTCA GACTACTACT GAAGGTCCCC AAACAACTGA TGCAGTTCAG
    TATGTAGAAT CTGACATTGT GTCAGAGGAA ACGTTGAAAT CCACTGGCAG AACTGCAGTT
    AGCTCAAACT CTACAAAAAT TGAGGCCGAT CTTTCCACAA CTCCTGAGGA CCTGACAGGA
    GTAAGTACCA ACACTCCTGA AATAGTCACT AGAGATTCCA TTACAATACT TATCCCAGAA
    ACCGAGGGCA TTACTAAAAA TCCTGTTAAA TCTGCACCTA TTACCATTCA GCATGTGGAT
    GTTGATGATG TTTTAGCAGC TACAGTGATG TCCACTCATA AACCTCCCAA AGTCTCACCT
    ACAGAAATAT CCCCCGATCA GAATCTCTTA CCAGAGGAAT CAAAGCTGCC AACTCCTACA
    AAACTAGCAG AAGTTTTGTC TGCCACAGAA GAGTTGCCAA AGGATCAGAC AGACAAGGAT
    GTTGCTAAAA TTGTATATGT GTCTACTGTG CCAGAACTGG TGTCACCAAC TATTCCCGCA
    TATACGTCAA CTGACACTAC TAAGGAAAAC CTACATGTGT TAACAGGGGG GGCAGTTGTT
    ACATCACTTG ATAAAGATGA GGAAGAACAT ACACCCCACA CAAAAGAGAG CAAAATTACA
    CAGGTGACAA CAGGAGCAGA ATTACCAGGT ACCATAACAG AGAGTACTCA TGGAATGTGG
    AGGGAAACCC AAGAGCCAAT AAATGTCACA CCTGATGCTG AAGACAATCA TTTGGGCAAA
    GAAGGGTACA TAATAACAGA GATCCCAGAA GTTGCTGAAG TACCAGCTGG AGTGACACCC
    AAAATGATTG TGGAAACTAC AACAGCCACT GAATCTGTGT TTACTGTGAA AGACACATCT
    ATAATTGTAG ACAAAACTGG CATTGTGAAA CCTGTCGTGA CTACGACAGA GGCAGCTGAA
    CTTACTGAGG TTGGATTCGA AGATTTAGGC ACAGACAAAG TAACAATTCT TGAAAACACA
    CTTGAAGATT CTGTAGGACT AACAAATGAA CCTTTTGCTG TATCATGGGG TGTAACTGAA
    GCCACTATTT TAGAGGAATC ATATGTGGAA AGTACTGAAA GTATTTCAGG TAAAGAGAAG
    TCCACTGAAA CTATTAAGAT CACAGGTGAA GCCACAACTG ATGACATTGG TACTTCTGAA
    ACTGACATTG TAGTGCCTGT AACCGATCGC ATAGAAGATA ATGTAAATGT ATCACCTAAA
    AATGATGATG CTAAGGAAAC AAACATTTTT GAAGACACAA CACCAGATCT TAGTGTTACT
    AATTCTGAAA AAGACATACT GACAACTACC ATACATAAAG ACATGCATGA AGTTACAGAA
    CCATTAAATA TTCCAGAGGT CACACATAAG GTTGTGATAG AGATCACAGA TGATGTACGT
    GAGACCAGAG GAGATTCCTT ACCCGAGACA CCAGAAGTCA CAACAGAGAC ACCTAAGGTT
    GCAACAGAAG CTGATGTCAA AACAACAACA AATGTTATAT CTAAAGCACC TGAAGTTATA
    CTGAAGACAA CTGTAACCAA GTCAGATACA CCTGATGTCA CAACTCAGAC ACCTTTGTTA
    AAGCTTGAAG ATACAGAGAG GGTTGAAATA GTACCTGAAA CTCCTGACAT ACAAGTGGTT
    CAAACAGAAC ACTCAGAAAA TGATATCCCA ACTCCTGTCT TGAAAGTGGA GGATTTCACA
    GTTGCGCTGA ATGACTTGAC AACTCCTATT CAAAAGACTT CTTCAACCAC ATTACAACAA
    GACATTTCTA ATGATATTTT AGAAGAAAAC AATGTGATGG GAAATGAAAT TGATGATATT
    GTGGTTCGAC CAGTACGCCC TGTCGGCGAT CATATTTTGG AGCTAAGTAT TAAGCTAAGA
    GGGGAAAGCT ATGACGATGC TCTGAGGGAT CCATCAAGTA ACTACTATCA ACATCTGTCT
    ACACTTTTTG TTGAAAAGAT TGAAGAAGTT TTCGAAAGGC TTCCAGGTTT CAAGAAAATA
    TATGTTCTTG AATTCAGGCC ACAGAAGGAC ATTGAAGGAG GTCTGGCAGT GGTGGTGCAC
    TATGCTGTTG TTCTTGAGGG TGATGGGTCA GGAGTAAGTA ATGAGACCAT GGATTACATA
    AACCTGCATT CCAACATGGT TGAGAACTCC TTCACAGATC CTGAGGAGCT GCCCACTGTG
    GTCTACACCA TCACTGACTT CCGAAACTAC ATCTCCGAAG CCCTTCACAA GGATAACTTT
    ATCAGAAACG CTACTCTGAA TGTGGACATT GATTCTTTGC AACTTGAAAA TGTGGAGACA
    TTGCTGCCTT CCAAACCAAC CAGCAGACCG TTTGACTCCA GTGACATGAT GGACAACGTT
    CTTGCTTCCG AGAAACCACC AGATGTTCCT GGGCAAGAAT TATCCAACAA TGACATCTTC
    ATTACAAATT ATGACATCTT TGATCCTGAT CCATGGACAG GTGGACAAAG TGAAGACAGA
    AATGCAAATG ATGTAATCAT CTTTGAGGAA AGTCCCACTC AGTCTCCTGT TGAAGTGTCT
    TTGAAGAACT TGGACATTGA AGTTACTTCA AAATCTGAAA CTTCAAGCAG TGCCCCTGTT
    CCTGATGAAG CTGTTATTGA AGAGGAGGGA TTTTTACAGA CTGCAACTAC AGTCAGTCCT
    ATTATAGACA CAATAACTGA ATTTACTTTA CCTACAGAAT CACCTCACAA GGTAGAAGAG
    ACACCAGTTT CTCCTGTAGA ACCTCCTGAG GTTGAGGAAA CTACCCTGGG TGATTTAACA
    GACCTTGGCT CAGGCTCAGG ATTCTCTGGA GATCACATAG GCCCTGACAT CTGGCCATGG
    TCATCAGAAA CATCACAAGA AGTCTTGAAG GAAGATACCA ATGATGAGAT ACACCAACTT
    CAGGAAAATC AGGAATCTAT AATAAAACAG CTTGAGCCTT TACATCAAGA TGTGACTCCT
    GAGGAGCCAT TCTTGGATAG GGTTCTTGTG ACCCAAGACA TTCGTACCAA TCCTCATTAC
    ACAACAACTG ACCAGGCCCC TGTATTTTGG ACAATGGAGA CTTTGACTGT TGAGCTTTCA
    ATGCAGACTC AAGTAGCCCC TGAACATTAT GATGACTATT TTCCAGATGC ATCAATCACT
    ATGGCTACAC TTGTAACCAA TCAACCATTG TTACATGTTC ACAATCCATT AGAAACAAAA
    GATCTGGATG CATCACAAAG TCCAGGAACA ATAAGTTCAA CTGCTGATAA GGACTATACA
    GAACGACTTG TTACACCCAC TGTGATCCAA CCAAGTGAAA CAGTAGCTGT GGATAAGCAT
    ACTACAACAG ACAACAAACA GAATCTGGAA ACTATGGGTA CATTATCTGC AACACTGGGC
    TCCACAATGA AGTCCACTGA AATCTCTTCA ACTACCCAAT TACCTGTGAT CATCAACAAT
    AATACAGTTT CAGTGGAAGG TCCTGCCAAT TCTTCAGAAG CTGTTCAGCA TTCTGTAAAT
    GAAGGAATCA CAGAGTTACC TGCTTTGGTC TGGCCAGATA TTGAAGGATC AGATGAAGAA
    TTCAAAGTTT TAGATGAGGA CATGGAACTC GTCAAGTTTG TGCCTACAAA AAGCCCAGTG
    ACTGAACTCT TAGAAGAAGA TTTAGTTGGT GATGAAATAT TGGTTGCAAC AACAACAACA
    ACAACAACTC TATCCTCAAC AACAGTAGCG ACTGTGGTAA CAAGTTTAGG TCATTCAGCC
    TCCCTGACTC CTGAAAAGGA CTCACCTTTC ACTTGGATAT CACATTCATC AATAGATGAG
    GATGAACCTG TGCAAGAGTC AACCACAGAA CCTCTACCCA CCCAATCACC CACTGCTTCT
    GTTCTTCCAA AGACAACTAC ACAGACATTG ATGCACACAT ACATAGCAGC TGTTGCAGGG
    GAGCCTGTTT TATCAGATAA CATGGATAAT AAGGACTCAA CCACAATGTC CACCCTTTTA
    GATGTTTCAA AACATTTAAA GACCCTTAGT TCCACCTCTG TACAATCATT TTCAGAACCA
    ACATTTACAC CTACTGTCCA CATAATTAAG CATGGAGAAA CTTCAGATGA CTCAGTACAA
    GATGTAGTTG AGACTGGAGA AGTTTTAGAT GACTCTGTAC AAGATGTACT TGAGCATGAA
    GACATTTCAT ATGGCTCTGT ACAAGATGTA GTTGTATCTG GAGAAACTTC AAATGACTCA
    GTACAAGATG TAGTTGAGAC TGGAGAAGTT TTAGATGATT CTGTACAAGA TGTACTTGAG
    CATGAGGACA TTTTATCTGG CCCAGTTCAA GATGTAGTTG TGTCTGGAGA AACTGCAGAA
    GACTCAGTAC AAGATTTAGT TGAGACTAGA GAAGTTTTAG ATGATTCTGT ACAAGATGTA
    CTTGAGCATG AGGACATTTT ATCTGGCCCA GTTCAAGATG TAGTTGTGTC TGGAGAAACT
    GCAGATGACT CAGTACAAGA TTTAGTTGAG ACTGGAGAAG TTTTAGATGA TTCCGTACAA
    GATGTACTTG AGCATGAAGT AATTTCATTT GGCCCAGTAC AAGATGTAGT TGTATCTGGA
    AAAACTTCAA ATGACTCAGT ACAAGATGTA GTTGAGAGTG CAAAAGATTC AGATGCCCCA
    ATACAAGATG TATTTGAGCA TAAAGACATT TCAGTTGACT CTGTACAAGA TGTAGTTGTG
    TCTGGAGAAA CTGCAGATGA CTCTATTCAA GATTTATTTA AGCATGAAGG CACTTCGTTC
    GACACAGTAC AAGATGAGGT TGAGTCTAGA GAAACTTCAG ATGACTCAGT ACAAAATGTA
    GTTGAGCATG AAGACTCTTC AGATGACGCA GTTCAAGATG TAGTCGAGTC TGGAGACACT
    TCAGATGACT CTGTACAAGA TATATTTGAG TCTGCAGAAA CTTCAGATGA CTCTGTACAA
    GATGTAGCTG AGACTGGAAA AGAATCAGAT GACTTAGTAC AAGATATATT TGAGAATAAA
    GACATTTCAG TTGACTCAGT ACAAGATGTA GTTGTATCTG GAGAGACTTC AGATGACTCT
    ATTCAAGGTT TACTTGAGCA TGAAGGCACT TCAGTCGACA TAGTACAAGA TGAGGTTGAG
    TTTAGAGAAA ATTCAGATGA CTCAGTACTA AATGTAGTCA AGCATGAAGA TACTTCAGAT
    GACTCAATAC AAGATGTAGT CGAGACAGGA GAAGTTTCAG ATAGCTCAGT ACAAGATGTA
    GCTGAGTCTG GAGAAACTTT AGATGACTCT GTTCAAGATT TTGTTAAAAC TGGAGAAATT
    TCCATTGACT CAGTACACAA TTTATTTGAG CCTGAAAAAA ACGTAGATGA CTCTGTACAA
    GATGTAGTTG AGACTGGAGA AGTTTCTGTT GATTCAGTAC AAGATGTAAT TGAGCTTGGA
    GACACTTTAG ATGACTCTGC ACAAGGAATA GTTGAACCTG AAGAAATGGC AGACAACTTT
    ATAAAGGAAA GCACTGTAAA GCCAAGACCT GACATCAAAG GGCTTATTCT ACCAACAGAG
    ACAGTGAACT ATGAAAGTGC CACTATGAAA GTTCCTGCTC CAGTTAACCC CAGCATAACA
    GAATTTAAGG TTTCATTTGA CATCTTCCCT TTCGACGACC CAACTGATGA TGAAGATAAT
    GGCAGTGGGT TTGCTCATGG AACTGACTTA GCAAGTTTAG CCTTGCCTGC CAGTCCTGGA
    AGGGCATTAA TAGTGTTTTT TAGCCTTAGG GTCACCAACA TGAAGTTTTC CGAAGATCTC
    TTCAACAAGA GCTCTACTGA ATACAAGGCA CTTGAGCAGC GATTTCTGGA ACTGCTCGTA
    CCGTATCTAC AGTCCAACCT CAGCAATTTC CAGAATTTAG AGATTTTGAA CTTCAGGAAT
    GGTAGCATTG TGGTGAACAG CAGGATGAAG TTTGCCAAGC CAGTTCCCCA TGGTGTGACA
    ACAGCAGTGT ACCTAATCTT GGAGGACTTC TGCAACACTG CCTACCAAAC TATGAATCTT
    GCAATAGACA AGTATTCATT GGATGTCGAA TCAGGTGACC AGGCAGATCC TTGTAAGTTT
    CAGGCATGTA ACGAGTTCTC CAAGTGTTTG GTGAACCGCT GGTCAGGTGA GGCCGAGTGT
    GTTTGTAACG CTGGTTATTT CAGCGTTGAC GGACTGCCAT GCCAGAGCAT CTGCGACATC
    CAAGAAGACT TCTGCCTAAA TGACGGGAAA TGTGACATAA TACCTGGCAA GGGCGCCATC
    TGCAGGTGTC GCGTTGGTGA AAACTGGTGG TATCGAGGAG AACACTGTGA GGAATATGTG
    TCAGAGCCGC TGGTGGTCGG CATTGCCATT GCGTCCGTTG CTGGATTTCT CCTGGTGGCG
    TCGGGTGTCA TCTTTTTCCT GGCCCGAACT CTGAGAGACC AGTATGACAC AGATGATTCA
    GAAGACCCTT TACGATATGC AGAGAACTTG CCATCTCTAG AAAGAGCCAC CAAGTACAAC
    CCGATGTTTG AAAGTGAAGT GACTGCAGGC CATAATCAGT ATTATCGGCG TTACCCAGAA
    GCTCCGGCAT TCAGCACGGG CAGCACCGAG ATCTCCACTG ACTTCAGCAG CGAAGAGATT
    CGTCACATCT ATCAGAACAG TGAACTTACT AAAGAGGAAA TCCAGGACCG TATTCGTATT
    ATAGAGCTTT ACGCCAAGGA CCGCCAGTTT GCAGATTTTG TCCGACAGCA CCAAGTGGCG
    CTTGACGCTA GACGAGAGAG CTCTTCCAAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45891
    Clone ID Related Accession (Same CDS sequence) XM_021474485.1 , XM_009296448.2 , XM_009296448.3 , XM_021473521.1
    Accession Version XM_021473521.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin homolog isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395115.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGCCTGATT TACCTTTTAA ATGCATTTGG TGGGGTTTGA CACTATTGTT GTTGACTGGA 
    TACTTTTCTA TAGATGCAGA TACTGGAACT CATGAGGATT ACTTGGCAAA TGGAAATACA
    ATGCTTTTGG ACACTAATGA AGTTTTGAAT CACTTATTGT TGTTGGACAA AGGTAAAACT
    TTGAATTACA TGTCTGCTGA AGGGAAAACA GCTCTATCTA GACATAAAAG GGACCTCCTG
    TTCCCCAATG GAGTTAAACT GTGCTCACAT GAGACGGTGC AACAGGCAGT GCAGAATCAT
    CTCAACTACT TTCACCTGCG AGTGTGCCAG GAGACAATTT GGGAGGCTTA TAAAATCTTC
    TGGGATCGCT TACCTGAGAA AGAAGAGTAC CAGATCTGGA TGAAAAAATG TCAAAATGGT
    AGCATTAGCG TGTTTGACAT CGGCCGAAGC TTTAGTCAGT CAGCTGAGCA CCTTGCTTTG
    ATTTCAAGTA GGGTAGCATC ATCCACAATA ACATCAACAA GGCATCAGAC GACGACTACT
    TCGCCAACAC TAGCAAAAGC TGAACAAGTT GGTATTTTGA TTCCTGAGGC AGTGCCAGGT
    TCACAAGTTG TCACACCGCA GACCTTTGCA GATGATATCG TAATCACAAC TTTGCCTACA
    AGGCTTCAAG TGACACCTGG AATTACCCCA AAGCCTGATC TTGTCACAGC CTTTAAGGAC
    ACAGTAAAAC CTATTTCTGA AACTGAACCT GAGGTCATAT TGGGTACTAC TTCTGAGAGT
    CCTGTTAAAG CATTGGATAC CATTACCATT AAACATGAAG AGGTTGTTGT AGTCTCAGAA
    TCTAAATTAT TGATTACTGA GAGGACTTCG GAGGACTCTA CTTCAAGGAA ATATGATTAT
    GAGTTTCCCA CGACCTTTAA GGACTCGTTA GTAATAAACA CTCCTATAGT ACTAACAACT
    GAATTTTCTG AACAAATGTA TTCAGAAATA GGACAAGGTT TTTCTGATGG TACAGTTAAA
    ACAGTAGCTG TTACTATTCA AAAGGAACTT GACATTGTCT CAAAATCTAC ATTATTAACC
    ACTGACAAAA CTCCAGAAGT TTCTACTTCA AAAAAATCTG AGCATGATAA TCCCACTAAC
    TTTGTAGATT CATTGGGAAT AAGCACCAAT ACTCCTAGAG TGACCACAAG TGAACTCAAA
    GAAGATCTTC ATTCAGAATT AGAACAGGTG GCTGGGCAGG ATGTTATTCA CAGTCCTGTT
    AAAACAACAC ATGTTACAAT TAAGCATGAA GTGTTGGACA CAAATACTAC ATTAATGACA
    ACTGACAGAA CTCCATATTC TACTTCAGCA AAATATGAAC GTGTTATACC AACATCCTTT
    GAGGACTCAC TAGTCACAAG TTCATCTACT GAACAGGATA TGACCAATGT TCCTATTAAG
    ACGGCATATG CCACAATTTC ACATGGAGAG TCTGAAATTG TCACAGAGTC GACAATAACA
    ACCACTAACA GAAATCTTGA AGATTCTTCT TTAGCAAAAT ATGATTATGG GATTCCTCAA
    GGAATTGATA CTTCTTCGGG AGTAATTAAT GAATCTTCTA CAAAAGAACA TAATTCAGTA
    TCTAAATTGA ATATTGGTCA GACTACTACT GAAGGTCCCC AAACAACTGA TGCAGTTCAG
    TATGTAGAAT CTGACATTGT GTCAGAGGAA ACGTTGAAAT CCACTGGCAG AACTGCAGTT
    AGCTCAAACT CTACAAAAAT TGAGGCCGAT CTTTCCACAA CTCCTGAGGA CCTGACAGGA
    GTAAGTACCA ACACTCCTGA AATAGTCACT AGAGATTCCA TTACAATACT TATCCCAGAA
    ACCGAGGGCA TTACTAAAAA TCCTGTTAAA TCTGCACCTA TTACCATTCA GCATGTGGAT
    GTTGATGATG TTTTAGCAGC TACAGTGATG TCCACTCATA AACCTCCCAA AGTCTCACCT
    ACAGAAATAT CCCCCGATCA GAATCTCTTA CCAGAGGAAT CAAAGCTGCC AACTCCTACA
    AAACTAGCAG AAGTTTTGTC TGCCACAGAA GAGTTGCCAA AGGATCAGAC AGACAAGGAT
    GTTGCTAAAA TTGTATATGT GTCTACTGTG CCAGAACTGG TGTCACCAAC TATTCCCGCA
    TATACGTCAA CTGACACTAC TAAGGAAAAC CTACATGTGT TAACAGGGGG GGCAGTTGTT
    ACATCACTTG ATAAAGATGA GGAAGAACAT ACACCCCACA CAAAAGAGAG CAAAATTACA
    CAGGTGACAA CAGGAGCAGA ATTACCAGGT ACCATAACAG AGAGTACTCA TGGAATGTGG
    AGGGAAACCC AAGAGCCAAT AAATGTCACA CCTGATGCTG AAGACAATCA TTTGGGCAAA
    GAAGGGTACA TAATAACAGA GATCCCAGAA GTTGCTGAAG TACCAGCTGG AGTGACACCC
    AAAATGATTG TGGAAACTAC AACAGCCACT GAATCTGTGT TTACTGTGAA AGACACATCT
    ATAATTGTAG ACAAAACTGG CATTGTGAAA CCTGTCGTGA CTACGACAGA GGCAGCTGAA
    CTTACTGAGG TTGGATTCGA AGATTTAGGC ACAGACAAAG TAACAATTCT TGAAAACACA
    CTTGAAGATT CTGTAGGACT AACAAATGAA CCTTTTGCTG TATCATGGGG TGTAACTGAA
    GCCACTATTT TAGAGGAATC ATATGTGGAA AGTACTGAAA GTATTTCAGG TAAAGAGAAG
    TCCACTGAAA CTATTAAGAT CACAGGTGAA GCCACAACTG ATGACATTGG TACTTCTGAA
    ACTGACATTG TAGTGCCTGT AACCGATCGC ATAGAAGATA ATGTAAATGT ATCACCTAAA
    AATGATGATG CTAAGGAAAC AAACATTTTT GAAGACACAA CACCAGATCT TAGTGTTACT
    AATTCTGAAA AAGACATACT GACAACTACC ATACATAAAG ACATGCATGA AGTTACAGAA
    CCATTAAATA TTCCAGAGGT CACACATAAG GTTGTGATAG AGATCACAGA TGATGTACGT
    GAGACCAGAG GAGATTCCTT ACCCGAGACA CCAGAAGTCA CAACAGAGAC ACCTAAGGTT
    GCAACAGAAG CTGATGTCAA AACAACAACA AATGTTATAT CTAAAGCACC TGAAGTTATA
    CTGAAGACAA CTGTAACCAA GTCAGATACA CCTGATGTCA CAACTCAGAC ACCTTTGTTA
    AAGCTTGAAG ATACAGAGAG GGTTGAAATA GTACCTGAAA CTCCTGACAT ACAAGTGGTT
    CAAACAGAAC ACTCAGAAAA TGATATCCCA ACTCCTGTCT TGAAAGTGGA GGATTTCACA
    GTTGCGCTGA ATGACTTGAC AACTCCTATT CAAAAGACTT CTTCAACCAC ATTACAACAA
    GACATTTCTA ATGATATTTT AGAAGAAAAC AATGTGATGG GAAATGAAAT TGATGATATT
    GTGGTTCGAC CAGTACGCCC TGTCGGCGAT CATATTTTGG AGCTAAGTAT TAAGCTAAGA
    GGGGAAAGCT ATGACGATGC TCTGAGGGAT CCATCAAGTA ACTACTATCA ACATCTGTCT
    ACACTTTTTG TTGAAAAGAT TGAAGAAGTT TTCGAAAGGC TTCCAGGTTT CAAGAAAATA
    TATGTTCTTG AATTCAGGCC ACAGAAGGAC ATTGAAGGAG GTCTGGCAGT GGTGGTGCAC
    TATGCTGTTG TTCTTGAGGG TGATGGGTCA GGAGTAAGTA ATGAGACCAT GGATTACATA
    AACCTGCATT CCAACATGGT TGAGAACTCC TTCACAGATC CTGAGGAGCT GCCCACTGTG
    GTCTACACCA TCACTGACTT CCGAAACTAC ATCTCCGAAG CCCTTCACAA GGATAACTTT
    ATCAGAAACG CTACTCTGAA TGTGGACATT GATTCTTTGC AACTTGAAAA TGTGGAGACA
    TTGCTGCCTT CCAAACCAAC CAGCAGACCG TTTGACTCCA GTGACATGAT GGACAACGTT
    CTTGCTTCCG AGAAACCACC AGATGTTCCT GGGCAAGAAT TATCCAACAA TGACATCTTC
    ATTACAAATT ATGACATCTT TGATCCTGAT CCATGGACAG GTGGACAAAG TGAAGACAGA
    AATGCAAATG ATGTAATCAT CTTTGAGGAA AGTCCCACTC AGTCTCCTGT TGAAGTGTCT
    TTGAAGAACT TGGACATTGA AGTTACTTCA AAATCTGAAA CTTCAAGCAG TGCCCCTGTT
    CCTGATGAAG CTGTTATTGA AGAGGAGGGA TTTTTACAGA CTGCAACTAC AGTCAGTCCT
    ATTATAGACA CAATAACTGA ATTTACTTTA CCTACAGAAT CACCTCACAA GGTAGAAGAG
    ACACCAGTTT CTCCTGTAGA ACCTCCTGAG GTTGAGGAAA CTACCCTGGG TGATTTAACA
    GACCTTGGCT CAGGCTCAGG ATTCTCTGGA GATCACATAG GCCCTGACAT CTGGCCATGG
    TCATCAGAAA CATCACAAGA AGTCTTGAAG GAAGATACCA ATGATGAGAT ACACCAACTT
    CAGGAAAATC AGGAATCTAT AATAAAACAG CTTGAGCCTT TACATCAAGA TGTGACTCCT
    GAGGAGCCAT TCTTGGATAG GGTTCTTGTG ACCCAAGACA TTCGTACCAA TCCTCATTAC
    ACAACAACTG ACCAGGCCCC TGTATTTTGG ACAATGGAGA CTTTGACTGT TGAGCTTTCA
    ATGCAGACTC AAGTAGCCCC TGAACATTAT GATGACTATT TTCCAGATGC ATCAATCACT
    ATGGCTACAC TTGTAACCAA TCAACCATTG TTACATGTTC ACAATCCATT AGAAACAAAA
    GATCTGGATG CATCACAAAG TCCAGGAACA ATAAGTTCAA CTGCTGATAA GGACTATACA
    GAACGACTTG TTACACCCAC TGTGATCCAA CCAAGTGAAA CAGTAGCTGT GGATAAGCAT
    ACTACAACAG ACAACAAACA GAATCTGGAA ACTATGGGTA CATTATCTGC AACACTGGGC
    TCCACAATGA AGTCCACTGA AATCTCTTCA ACTACCCAAT TACCTGTGAT CATCAACAAT
    AATACAGTTT CAGTGGAAGG TCCTGCCAAT TCTTCAGAAG CTGTTCAGCA TTCTGTAAAT
    GAAGGAATCA CAGAGTTACC TGCTTTGGTC TGGCCAGATA TTGAAGGATC AGATGAAGAA
    TTCAAAGTTT TAGATGAGGA CATGGAACTC GTCAAGTTTG TGCCTACAAA AAGCCCAGTG
    ACTGAACTCT TAGAAGAAGA TTTAGTTGGT GATGAAATAT TGGTTGCAAC AACAACAACA
    ACAACAACTC TATCCTCAAC AACAGTAGCG ACTGTGGTAA CAAGTTTAGG TCATTCAGCC
    TCCCTGACTC CTGAAAAGGA CTCACCTTTC ACTTGGATAT CACATTCATC AATAGATGAG
    GATGAACCTG TGCAAGAGTC AACCACAGAA CCTCTACCCA CCCAATCACC CACTGCTTCT
    GTTCTTCCAA AGACAACTAC ACAGACATTG ATGCACACAT ACATAGCAGC TGTTGCAGGG
    GAGCCTGTTT TATCAGATAA CATGGATAAT AAGGACTCAA CCACAATGTC CACCCTTTTA
    GATGTTTCAA AACATTTAAA GACCCTTAGT TCCACCTCTG TACAATCATT TTCAGAACCA
    ACATTTACAC CTACTGTCCA CATAATTAAG CATGGAGAAA CTTCAGATGA CTCAGTACAA
    GATGTAGTTG AGACTGGAGA AGTTTTAGAT GACTCTGTAC AAGATGTACT TGAGCATGAA
    GACATTTCAT ATGGCTCTGT ACAAGATGTA GTTGTATCTG GAGAAACTTC AAATGACTCA
    GTACAAGATG TAGTTGAGAC TGGAGAAGTT TTAGATGATT CTGTACAAGA TGTACTTGAG
    CATGAGGACA TTTTATCTGG CCCAGTTCAA GATGTAGTTG TGTCTGGAGA AACTGCAGAA
    GACTCAGTAC AAGATTTAGT TGAGACTAGA GAAGTTTTAG ATGATTCTGT ACAAGATGTA
    CTTGAGCATG AGGACATTTT ATCTGGCCCA GTTCAAGATG TAGTTGTGTC TGGAGAAACT
    GCAGATGACT CAGTACAAGA TTTAGTTGAG ACTGGAGAAG TTTTAGATGA TTCCGTACAA
    GATGTACTTG AGCATGAAGT AATTTCATTT GGCCCAGTAC AAGATGTAGT TGTATCTGGA
    AAAACTTCAA ATGACTCAGT ACAAGATGTA GTTGAGAGTG CAAAAGATTC AGATGCCCCA
    ATACAAGATG TATTTGAGCA TAAAGACATT TCAGTTGACT CTGTACAAGA TGTAGTTGTG
    TCTGGAGAAA CTGCAGATGA CTCTATTCAA GATTTATTTA AGCATGAAGG CACTTCGTTC
    GACACAGTAC AAGATGAGGT TGAGTCTAGA GAAACTTCAG ATGACTCAGT ACAAAATGTA
    GTTGAGCATG AAGACTCTTC AGATGACGCA GTTCAAGATG TAGTCGAGTC TGGAGACACT
    TCAGATGACT CTGTACAAGA TATATTTGAG TCTGCAGAAA CTTCAGATGA CTCTGTACAA
    GATGTAGCTG AGACTGGAAA AGAATCAGAT GACTTAGTAC AAGATATATT TGAGAATAAA
    GACATTTCAG TTGACTCAGT ACAAGATGTA GTTGTATCTG GAGAGACTTC AGATGACTCT
    ATTCAAGGTT TACTTGAGCA TGAAGGCACT TCAGTCGACA TAGTACAAGA TGAGGTTGAG
    TTTAGAGAAA ATTCAGATGA CTCAGTACTA AATGTAGTCA AGCATGAAGA TACTTCAGAT
    GACTCAATAC AAGATGTAGT CGAGACAGGA GAAGTTTCAG ATAGCTCAGT ACAAGATGTA
    GCTGAGTCTG GAGAAACTTT AGATGACTCT GTTCAAGATT TTGTTAAAAC TGGAGAAATT
    TCCATTGACT CAGTACACAA TTTATTTGAG CCTGAAAAAA ACGTAGATGA CTCTGTACAA
    GATGTAGTTG AGACTGGAGA AGTTTCTGTT GATTCAGTAC AAGATGTAAT TGAGCTTGGA
    GACACTTTAG ATGACTCTGC ACAAGGAATA GTTGAACCTG AAGAAATGGC AGACAACTTT
    ATAAAGGAAA GCACTGTAAA GCCAAGACCT GACATCAAAG GGCTTATTCT ACCAACAGAG
    ACAGTGAACT ATGAAAGTGC CACTATGAAA GTTCCTGCTC CAGTTAACCC CAGCATAACA
    GAATTTAAGG TTTCATTTGA CATCTTCCCT TTCGACGACC CAACTGATGA TGAAGATAAT
    GGCAGTGGGT TTGCTCATGG AACTGACTTA GCAAGTTTAG CCTTGCCTGC CAGTCCTGGA
    AGGGCATTAA TAGTGTTTTT TAGCCTTAGG GTCACCAACA TGAAGTTTTC CGAAGATCTC
    TTCAACAAGA GCTCTACTGA ATACAAGGCA CTTGAGCAGC GATTTCTGGA ACTGCTCGTA
    CCGTATCTAC AGTCCAACCT CAGCAATTTC CAGAATTTAG AGATTTTGAA CTTCAGGAAT
    GGTAGCATTG TGGTGAACAG CAGGATGAAG TTTGCCAAGC CAGTTCCCCA TGGTGTGACA
    ACAGCAGTGT ACCTAATCTT GGAGGACTTC TGCAACACTG CCTACCAAAC TATGAATCTT
    GCAATAGACA AGTATTCATT GGATGTCGAA TCAGGTGACC AGGCAGATCC TTGTAAGTTT
    CAGGCATGTA ACGAGTTCTC CAAGTGTTTG GTGAACCGCT GGTCAGGTGA GGCCGAGTGT
    GTTTGTAACG CTGGTTATTT CAGCGTTGAC GGACTGCCAT GCCAGAGCAT CTGCGACATC
    CAAGAAGACT TCTGCCTAAA TGACGGGAAA TGTGACATAA TACCTGGCAA GGGCGCCATC
    TGCAGGTGTC GCGTTGGTGA AAACTGGTGG TATCGAGGAG AACACTGTGA GGAATATGTG
    TCAGAGCCGC TGGTGGTCGG CATTGCCATT GCGTCCGTTG CTGGATTTCT CCTGGTGGCG
    TCGGGTGTCA TCTTTTTCCT GGCCCGAACT CTGAGAGACC AGTATGACAC AGATGATTCA
    GAAGACCCTT TACGATATGC AGAGAACTTG CCATCTCTAG AAAGAGCCAC CAAGTACAAC
    CCGATGTTTG AAAGTGAAGT GACTGCAGGC CATAATCAGT ATTATCGGCG TTACCCAGAA
    GCTCCGGCAT TCAGCACGGG CAGCACCGAG ATCTCCACTG ACTTCAGCAG CGAAGAGATT
    CGTCACATCT ATCAGAACAG TGAACTTACT AAAGAGGAAA TCCAGGACCG TATTCGTATT
    ATAGAGCTTT ACGCCAAGGA CCGCCAGTTT GCAGATTTTG TCCGACAGCA CCAAGTGGCG
    CTTGACGCTA GACGAGAGAG CTCTTCCAAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329196.1
    CDS233..8665
    Translation

    Target ORF information:

    RefSeq Version XM_021473521.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio interphotoreceptor matrix proteoglycan 2b (impg2b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473521.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGCCTGATT TACCTTTTAA ATGCATTTGG TGGGGTTTGA CACTATTGTT GTTGACTGGA 
    TACTTTTCTA TAGATGCAGA TACTGGAACT CATGAGGATT ACTTGGCAAA TGGAAATACA
    ATGCTTTTGG ACACTAATGA AGTTTTGAAT CACTTATTGT TGTTGGACAA AGGTAAAACT
    TTGAATTACA TGTCTGCTGA AGGGAAAACA GCTCTATCTA GACATAAAAG GGACCTCCTG
    TTCCCCAATG GAGTTAAACT GTGCTCACAT GAGACGGTGC AACAGGCAGT GCAGAATCAT
    CTCAACTACT TTCACCTGCG AGTGTGCCAG GAGACAATTT GGGAGGCTTA TAAAATCTTC
    TGGGATCGCT TACCTGAGAA AGAAGAGTAC CAGATCTGGA TGAAAAAATG TCAAAATGGT
    AGCATTAGCG TGTTTGACAT CGGCCGAAGC TTTAGTCAGT CAGCTGAGCA CCTTGCTTTG
    ATTTCAAGTA GGGTAGCATC ATCCACAATA ACATCAACAA GGCATCAGAC GACGACTACT
    TCGCCAACAC TAGCAAAAGC TGAACAAGTT GGTATTTTGA TTCCTGAGGC AGTGCCAGGT
    TCACAAGTTG TCACACCGCA GACCTTTGCA GATGATATCG TAATCACAAC TTTGCCTACA
    AGGCTTCAAG TGACACCTGG AATTACCCCA AAGCCTGATC TTGTCACAGC CTTTAAGGAC
    ACAGTAAAAC CTATTTCTGA AACTGAACCT GAGGTCATAT TGGGTACTAC TTCTGAGAGT
    CCTGTTAAAG CATTGGATAC CATTACCATT AAACATGAAG AGGTTGTTGT AGTCTCAGAA
    TCTAAATTAT TGATTACTGA GAGGACTTCG GAGGACTCTA CTTCAAGGAA ATATGATTAT
    GAGTTTCCCA CGACCTTTAA GGACTCGTTA GTAATAAACA CTCCTATAGT ACTAACAACT
    GAATTTTCTG AACAAATGTA TTCAGAAATA GGACAAGGTT TTTCTGATGG TACAGTTAAA
    ACAGTAGCTG TTACTATTCA AAAGGAACTT GACATTGTCT CAAAATCTAC ATTATTAACC
    ACTGACAAAA CTCCAGAAGT TTCTACTTCA AAAAAATCTG AGCATGATAA TCCCACTAAC
    TTTGTAGATT CATTGGGAAT AAGCACCAAT ACTCCTAGAG TGACCACAAG TGAACTCAAA
    GAAGATCTTC ATTCAGAATT AGAACAGGTG GCTGGGCAGG ATGTTATTCA CAGTCCTGTT
    AAAACAACAC ATGTTACAAT TAAGCATGAA GTGTTGGACA CAAATACTAC ATTAATGACA
    ACTGACAGAA CTCCATATTC TACTTCAGCA AAATATGAAC GTGTTATACC AACATCCTTT
    GAGGACTCAC TAGTCACAAG TTCATCTACT GAACAGGATA TGACCAATGT TCCTATTAAG
    ACGGCATATG CCACAATTTC ACATGGAGAG TCTGAAATTG TCACAGAGTC GACAATAACA
    ACCACTAACA GAAATCTTGA AGATTCTTCT TTAGCAAAAT ATGATTATGG GATTCCTCAA
    GGAATTGATA CTTCTTCGGG AGTAATTAAT GAATCTTCTA CAAAAGAACA TAATTCAGTA
    TCTAAATTGA ATATTGGTCA GACTACTACT GAAGGTCCCC AAACAACTGA TGCAGTTCAG
    TATGTAGAAT CTGACATTGT GTCAGAGGAA ACGTTGAAAT CCACTGGCAG AACTGCAGTT
    AGCTCAAACT CTACAAAAAT TGAGGCCGAT CTTTCCACAA CTCCTGAGGA CCTGACAGGA
    GTAAGTACCA ACACTCCTGA AATAGTCACT AGAGATTCCA TTACAATACT TATCCCAGAA
    ACCGAGGGCA TTACTAAAAA TCCTGTTAAA TCTGCACCTA TTACCATTCA GCATGTGGAT
    GTTGATGATG TTTTAGCAGC TACAGTGATG TCCACTCATA AACCTCCCAA AGTCTCACCT
    ACAGAAATAT CCCCCGATCA GAATCTCTTA CCAGAGGAAT CAAAGCTGCC AACTCCTACA
    AAACTAGCAG AAGTTTTGTC TGCCACAGAA GAGTTGCCAA AGGATCAGAC AGACAAGGAT
    GTTGCTAAAA TTGTATATGT GTCTACTGTG CCAGAACTGG TGTCACCAAC TATTCCCGCA
    TATACGTCAA CTGACACTAC TAAGGAAAAC CTACATGTGT TAACAGGGGG GGCAGTTGTT
    ACATCACTTG ATAAAGATGA GGAAGAACAT ACACCCCACA CAAAAGAGAG CAAAATTACA
    CAGGTGACAA CAGGAGCAGA ATTACCAGGT ACCATAACAG AGAGTACTCA TGGAATGTGG
    AGGGAAACCC AAGAGCCAAT AAATGTCACA CCTGATGCTG AAGACAATCA TTTGGGCAAA
    GAAGGGTACA TAATAACAGA GATCCCAGAA GTTGCTGAAG TACCAGCTGG AGTGACACCC
    AAAATGATTG TGGAAACTAC AACAGCCACT GAATCTGTGT TTACTGTGAA AGACACATCT
    ATAATTGTAG ACAAAACTGG CATTGTGAAA CCTGTCGTGA CTACGACAGA GGCAGCTGAA
    CTTACTGAGG TTGGATTCGA AGATTTAGGC ACAGACAAAG TAACAATTCT TGAAAACACA
    CTTGAAGATT CTGTAGGACT AACAAATGAA CCTTTTGCTG TATCATGGGG TGTAACTGAA
    GCCACTATTT TAGAGGAATC ATATGTGGAA AGTACTGAAA GTATTTCAGG TAAAGAGAAG
    TCCACTGAAA CTATTAAGAT CACAGGTGAA GCCACAACTG ATGACATTGG TACTTCTGAA
    ACTGACATTG TAGTGCCTGT AACCGATCGC ATAGAAGATA ATGTAAATGT ATCACCTAAA
    AATGATGATG CTAAGGAAAC AAACATTTTT GAAGACACAA CACCAGATCT TAGTGTTACT
    AATTCTGAAA AAGACATACT GACAACTACC ATACATAAAG ACATGCATGA AGTTACAGAA
    CCATTAAATA TTCCAGAGGT CACACATAAG GTTGTGATAG AGATCACAGA TGATGTACGT
    GAGACCAGAG GAGATTCCTT ACCCGAGACA CCAGAAGTCA CAACAGAGAC ACCTAAGGTT
    GCAACAGAAG CTGATGTCAA AACAACAACA AATGTTATAT CTAAAGCACC TGAAGTTATA
    CTGAAGACAA CTGTAACCAA GTCAGATACA CCTGATGTCA CAACTCAGAC ACCTTTGTTA
    AAGCTTGAAG ATACAGAGAG GGTTGAAATA GTACCTGAAA CTCCTGACAT ACAAGTGGTT
    CAAACAGAAC ACTCAGAAAA TGATATCCCA ACTCCTGTCT TGAAAGTGGA GGATTTCACA
    GTTGCGCTGA ATGACTTGAC AACTCCTATT CAAAAGACTT CTTCAACCAC ATTACAACAA
    GACATTTCTA ATGATATTTT AGAAGAAAAC AATGTGATGG GAAATGAAAT TGATGATATT
    GTGGTTCGAC CAGTACGCCC TGTCGGCGAT CATATTTTGG AGCTAAGTAT TAAGCTAAGA
    GGGGAAAGCT ATGACGATGC TCTGAGGGAT CCATCAAGTA ACTACTATCA ACATCTGTCT
    ACACTTTTTG TTGAAAAGAT TGAAGAAGTT TTCGAAAGGC TTCCAGGTTT CAAGAAAATA
    TATGTTCTTG AATTCAGGCC ACAGAAGGAC ATTGAAGGAG GTCTGGCAGT GGTGGTGCAC
    TATGCTGTTG TTCTTGAGGG TGATGGGTCA GGAGTAAGTA ATGAGACCAT GGATTACATA
    AACCTGCATT CCAACATGGT TGAGAACTCC TTCACAGATC CTGAGGAGCT GCCCACTGTG
    GTCTACACCA TCACTGACTT CCGAAACTAC ATCTCCGAAG CCCTTCACAA GGATAACTTT
    ATCAGAAACG CTACTCTGAA TGTGGACATT GATTCTTTGC AACTTGAAAA TGTGGAGACA
    TTGCTGCCTT CCAAACCAAC CAGCAGACCG TTTGACTCCA GTGACATGAT GGACAACGTT
    CTTGCTTCCG AGAAACCACC AGATGTTCCT GGGCAAGAAT TATCCAACAA TGACATCTTC
    ATTACAAATT ATGACATCTT TGATCCTGAT CCATGGACAG GTGGACAAAG TGAAGACAGA
    AATGCAAATG ATGTAATCAT CTTTGAGGAA AGTCCCACTC AGTCTCCTGT TGAAGTGTCT
    TTGAAGAACT TGGACATTGA AGTTACTTCA AAATCTGAAA CTTCAAGCAG TGCCCCTGTT
    CCTGATGAAG CTGTTATTGA AGAGGAGGGA TTTTTACAGA CTGCAACTAC AGTCAGTCCT
    ATTATAGACA CAATAACTGA ATTTACTTTA CCTACAGAAT CACCTCACAA GGTAGAAGAG
    ACACCAGTTT CTCCTGTAGA ACCTCCTGAG GTTGAGGAAA CTACCCTGGG TGATTTAACA
    GACCTTGGCT CAGGCTCAGG ATTCTCTGGA GATCACATAG GCCCTGACAT CTGGCCATGG
    TCATCAGAAA CATCACAAGA AGTCTTGAAG GAAGATACCA ATGATGAGAT ACACCAACTT
    CAGGAAAATC AGGAATCTAT AATAAAACAG CTTGAGCCTT TACATCAAGA TGTGACTCCT
    GAGGAGCCAT TCTTGGATAG GGTTCTTGTG ACCCAAGACA TTCGTACCAA TCCTCATTAC
    ACAACAACTG ACCAGGCCCC TGTATTTTGG ACAATGGAGA CTTTGACTGT TGAGCTTTCA
    ATGCAGACTC AAGTAGCCCC TGAACATTAT GATGACTATT TTCCAGATGC ATCAATCACT
    ATGGCTACAC TTGTAACCAA TCAACCATTG TTACATGTTC ACAATCCATT AGAAACAAAA
    GATCTGGATG CATCACAAAG TCCAGGAACA ATAAGTTCAA CTGCTGATAA GGACTATACA
    GAACGACTTG TTACACCCAC TGTGATCCAA CCAAGTGAAA CAGTAGCTGT GGATAAGCAT
    ACTACAACAG ACAACAAACA GAATCTGGAA ACTATGGGTA CATTATCTGC AACACTGGGC
    TCCACAATGA AGTCCACTGA AATCTCTTCA ACTACCCAAT TACCTGTGAT CATCAACAAT
    AATACAGTTT CAGTGGAAGG TCCTGCCAAT TCTTCAGAAG CTGTTCAGCA TTCTGTAAAT
    GAAGGAATCA CAGAGTTACC TGCTTTGGTC TGGCCAGATA TTGAAGGATC AGATGAAGAA
    TTCAAAGTTT TAGATGAGGA CATGGAACTC GTCAAGTTTG TGCCTACAAA AAGCCCAGTG
    ACTGAACTCT TAGAAGAAGA TTTAGTTGGT GATGAAATAT TGGTTGCAAC AACAACAACA
    ACAACAACTC TATCCTCAAC AACAGTAGCG ACTGTGGTAA CAAGTTTAGG TCATTCAGCC
    TCCCTGACTC CTGAAAAGGA CTCACCTTTC ACTTGGATAT CACATTCATC AATAGATGAG
    GATGAACCTG TGCAAGAGTC AACCACAGAA CCTCTACCCA CCCAATCACC CACTGCTTCT
    GTTCTTCCAA AGACAACTAC ACAGACATTG ATGCACACAT ACATAGCAGC TGTTGCAGGG
    GAGCCTGTTT TATCAGATAA CATGGATAAT AAGGACTCAA CCACAATGTC CACCCTTTTA
    GATGTTTCAA AACATTTAAA GACCCTTAGT TCCACCTCTG TACAATCATT TTCAGAACCA
    ACATTTACAC CTACTGTCCA CATAATTAAG CATGGAGAAA CTTCAGATGA CTCAGTACAA
    GATGTAGTTG AGACTGGAGA AGTTTTAGAT GACTCTGTAC AAGATGTACT TGAGCATGAA
    GACATTTCAT ATGGCTCTGT ACAAGATGTA GTTGTATCTG GAGAAACTTC AAATGACTCA
    GTACAAGATG TAGTTGAGAC TGGAGAAGTT TTAGATGATT CTGTACAAGA TGTACTTGAG
    CATGAGGACA TTTTATCTGG CCCAGTTCAA GATGTAGTTG TGTCTGGAGA AACTGCAGAA
    GACTCAGTAC AAGATTTAGT TGAGACTAGA GAAGTTTTAG ATGATTCTGT ACAAGATGTA
    CTTGAGCATG AGGACATTTT ATCTGGCCCA GTTCAAGATG TAGTTGTGTC TGGAGAAACT
    GCAGATGACT CAGTACAAGA TTTAGTTGAG ACTGGAGAAG TTTTAGATGA TTCCGTACAA
    GATGTACTTG AGCATGAAGT AATTTCATTT GGCCCAGTAC AAGATGTAGT TGTATCTGGA
    AAAACTTCAA ATGACTCAGT ACAAGATGTA GTTGAGAGTG CAAAAGATTC AGATGCCCCA
    ATACAAGATG TATTTGAGCA TAAAGACATT TCAGTTGACT CTGTACAAGA TGTAGTTGTG
    TCTGGAGAAA CTGCAGATGA CTCTATTCAA GATTTATTTA AGCATGAAGG CACTTCGTTC
    GACACAGTAC AAGATGAGGT TGAGTCTAGA GAAACTTCAG ATGACTCAGT ACAAAATGTA
    GTTGAGCATG AAGACTCTTC AGATGACGCA GTTCAAGATG TAGTCGAGTC TGGAGACACT
    TCAGATGACT CTGTACAAGA TATATTTGAG TCTGCAGAAA CTTCAGATGA CTCTGTACAA
    GATGTAGCTG AGACTGGAAA AGAATCAGAT GACTTAGTAC AAGATATATT TGAGAATAAA
    GACATTTCAG TTGACTCAGT ACAAGATGTA GTTGTATCTG GAGAGACTTC AGATGACTCT
    ATTCAAGGTT TACTTGAGCA TGAAGGCACT TCAGTCGACA TAGTACAAGA TGAGGTTGAG
    TTTAGAGAAA ATTCAGATGA CTCAGTACTA AATGTAGTCA AGCATGAAGA TACTTCAGAT
    GACTCAATAC AAGATGTAGT CGAGACAGGA GAAGTTTCAG ATAGCTCAGT ACAAGATGTA
    GCTGAGTCTG GAGAAACTTT AGATGACTCT GTTCAAGATT TTGTTAAAAC TGGAGAAATT
    TCCATTGACT CAGTACACAA TTTATTTGAG CCTGAAAAAA ACGTAGATGA CTCTGTACAA
    GATGTAGTTG AGACTGGAGA AGTTTCTGTT GATTCAGTAC AAGATGTAAT TGAGCTTGGA
    GACACTTTAG ATGACTCTGC ACAAGGAATA GTTGAACCTG AAGAAATGGC AGACAACTTT
    ATAAAGGAAA GCACTGTAAA GCCAAGACCT GACATCAAAG GGCTTATTCT ACCAACAGAG
    ACAGTGAACT ATGAAAGTGC CACTATGAAA GTTCCTGCTC CAGTTAACCC CAGCATAACA
    GAATTTAAGG TTTCATTTGA CATCTTCCCT TTCGACGACC CAACTGATGA TGAAGATAAT
    GGCAGTGGGT TTGCTCATGG AACTGACTTA GCAAGTTTAG CCTTGCCTGC CAGTCCTGGA
    AGGGCATTAA TAGTGTTTTT TAGCCTTAGG GTCACCAACA TGAAGTTTTC CGAAGATCTC
    TTCAACAAGA GCTCTACTGA ATACAAGGCA CTTGAGCAGC GATTTCTGGA ACTGCTCGTA
    CCGTATCTAC AGTCCAACCT CAGCAATTTC CAGAATTTAG AGATTTTGAA CTTCAGGAAT
    GGTAGCATTG TGGTGAACAG CAGGATGAAG TTTGCCAAGC CAGTTCCCCA TGGTGTGACA
    ACAGCAGTGT ACCTAATCTT GGAGGACTTC TGCAACACTG CCTACCAAAC TATGAATCTT
    GCAATAGACA AGTATTCATT GGATGTCGAA TCAGGTGACC AGGCAGATCC TTGTAAGTTT
    CAGGCATGTA ACGAGTTCTC CAAGTGTTTG GTGAACCGCT GGTCAGGTGA GGCCGAGTGT
    GTTTGTAACG CTGGTTATTT CAGCGTTGAC GGACTGCCAT GCCAGAGCAT CTGCGACATC
    CAAGAAGACT TCTGCCTAAA TGACGGGAAA TGTGACATAA TACCTGGCAA GGGCGCCATC
    TGCAGGTGTC GCGTTGGTGA AAACTGGTGG TATCGAGGAG AACACTGTGA GGAATATGTG
    TCAGAGCCGC TGGTGGTCGG CATTGCCATT GCGTCCGTTG CTGGATTTCT CCTGGTGGCG
    TCGGGTGTCA TCTTTTTCCT GGCCCGAACT CTGAGAGACC AGTATGACAC AGATGATTCA
    GAAGACCCTT TACGATATGC AGAGAACTTG CCATCTCTAG AAAGAGCCAC CAAGTACAAC
    CCGATGTTTG AAAGTGAAGT GACTGCAGGC CATAATCAGT ATTATCGGCG TTACCCAGAA
    GCTCCGGCAT TCAGCACGGG CAGCACCGAG ATCTCCACTG ACTTCAGCAG CGAAGAGATT
    CGTCACATCT ATCAGAACAG TGAACTTACT AAAGAGGAAA TCCAGGACCG TATTCGTATT
    ATAGAGCTTT ACGCCAAGGA CCGCCAGTTT GCAGATTTTG TCCGACAGCA CCAAGTGGCG
    CTTGACGCTA GACGAGAGAG CTCTTCCAAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.