shank1 gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_021471433.1	XP_021327108.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X2
XM_021471439.1	XP_021327114.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X8
XM_021474082.1	XP_021329757.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X7
XM_009299490.2	XP_009297765.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X3
XM_009299493.2	XP_009297768.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X6
XM_009299497.2	XP_009297772.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X10
XM_009299485.2	XP_009297760.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X1
XM_009299491.2	XP_009297766.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X4
XM_021471431.1	XP_021327106.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X1
XM_021471434.1	XP_021327109.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X3
XM_021471438.1	XP_021327113.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X7
XM_021474073.1	XP_021329748.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X3
XM_021474080.1	XP_021329755.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X5
XM_009299496.2	XP_009297771.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X9
XM_021474051.1	XP_021329726.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X1
XM_021474081.1	XP_021329756.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X6
XM_009299487.2	XP_009297762.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X1
XM_021474062.1	XP_021329737.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X2
XM_021474077.1	XP_021329752.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X4
XM_009299494.2	XP_009297769.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X7
XM_009299495.2	XP_009297770.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X8
XM_009299484.2	XP_009297759.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X1
XM_009299489.2	XP_009297764.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X2
XM_009299492.2	XP_009297767.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X5
XM_021474058.1	XP_021329733.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X1
XM_021474085.1	XP_021329760.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X8
XM_021471432.1	XP_021327107.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X1
XM_021471435.1	XP_021327110.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X4
XM_021471436.1	XP_021327111.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X5
XM_021471437.1	XP_021327112.1	SH3 and multiple ankyrin repeat domains protein 1 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa45597	XM_009299494.2 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00
ODa45598	XM_009299495.2 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00
ODa141768	XM_021474080.1 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa141768	XM_021471436.1 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa141769	XM_021471437.1 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa141769	XM_021474081.1 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa141764	XM_021471431.1 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa141764	XM_021474051.1 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa141764	XM_021474058.1 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa141764	XM_021471432.1 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa141765	XM_021471433.1 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa141765	XM_021474062.1 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa141766	XM_021471434.1 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa141766	XM_021474073.1 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa141767	XM_021474077.1 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa141767	XM_021471435.1 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa141770	XM_021474082.1 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
ODa141770	XM_021471438.1 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
ODa141771	XM_021474085.1 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
ODa141771	XM_021471439.1 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
ODa45591	XM_009299485.2 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa45591	XM_009299484.2 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa45591	XM_009299487.2 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa45592	XM_009299489.2 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa45593	XM_009299490.2 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa45594	XM_009299491.2 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa45595	XM_009299492.2 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa45596	XM_009299493.2 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa45599	XM_009299496.2 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
ODa45600	XM_009299497.2 Latest version!	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

shank1 ( XM_009299494.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297769.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ODa45597
Clone ID Related Accession (Same CDS sequence)	XM_009299494.2
Accession Version	XM_009299494.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299494.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATGG GAGGATGCGG AGAGGAGGAT CTCTCTCTCT CCCTCTCCCT CTCGTCGTCC 
TGGCCATCGC TAGGCCCTAT GAATCGCAGC GTCTGGTTCA TTTACAGTGA CTACATAATC 
AAGGAGAAGA CAGTCCTCCT GCAGAAAAAA GACAATGAAG GTTTTGGCTT TGTGCTGAGA 
GGAGCCAAGG CTCAGACCCC TATAGAAGAG TTCACTCCCA CCCCAGCCTT TCCAGCACTG 
CAGTACCTTG AGTCTGTCGA CGAGGGAGGC GTGGCCTGGA GAGCTGGTCT GCGAATGGGA 
GACTTCCTTA TAGAGGTGAA TGGTCAGAAT GTGGTTAAGG TGGGACACAG GCAGGTGGTC 
AATATGATCA GGCAAGGCGG TAACAGTCTC ATGGTCAAGG TTGTCATGGT TACTCGAAAT 
CCTGATATGG AGGAAGGGGT CAGAAAGAAA ATCCCACAGC AGAGTAAAAG ACTGAGCACA 
CCGGCTATCG CTCTGCGCTC CAAATCCATG ACATCAGAGC TGGAAGAGAT GGTGGACAGA 
GCCTCTGCCC CATGGAAAAA AAAATCAGAG TTCGAATCTT CACAAGCTAC AGAGAAGAAG 
AGGACAGTTT ATCAGATGGC TTTGAATAAA CTGGATGAGA TTTTAGCAGC AGCACAGCAG 
ACCATTAATA CAAATGAGGC TCCAGGGCCC CGGGTTCAGG GGGTAAAAAG AGAACGGGGC 
AGAGGCTTTT ATAATGAGCC AAATTTCGAC CAAACTGGAA TGGGGATGAC ATCATCTGGG 
GCAGGGTTAG GCTACGACCG TGGTCAATTT GTGTCTGGCC ATGGCCCCCC GCACGGTATG 
CTGCGACAGA AGTCTATCGG GGTTCCTGAG GAGGAGAAGC AGTTTCTTCA TCCTCCAGCC 
ATGAAGTTTG CCCGTAGTCT TTCAGTACCT GGCCCAGATG AAATCCCTCC TCCACCTACT 
ACAGCACCCC CTGAGCCCCC TTTTTCCTCA GCTCCTCCGC TTGGCTTTAG GGGCAAGACA 
TCTCAACAGC CGTCTGTTTC TATGTCTCAG TCTACTTCCT CTGCTGCACA TTATCAACTC 
TACTCACAGT CAGTACATGT TACTCACGGA CCAAGGGACA GGTCCCCTGG ACCACCTACT 
GGGTCTGGAA CTGGGGACCT TGCTTATTCC CATGCCCAAG CCCACCCCTC TGTACCAAGT 
AGGACTGCCT CCAGGAAGGT GATTGGGTAC ACAGGAGATC TTGCAGGGGC TGGAGGAGTG 
GCTGGGGCCA AAGCTGCAGC ATTGAGAAGG GGTTACAGCA ATGCAGTCCC ACCCACCAGT 
GTCGCTACTG TGATTCCACA ACAGCAGCAG ACTACTCAGA ACCAACCTTC CAGAATGGAC 
CGGAGTGGAA TGGGGGTAGG GGCTGGTGGT GTACCAAAAG GCGGTGCTCG TAGAGGAAAG 
GGGCCCTTGG TGAAACAGTC AAAGGTGGAA GACCTGCGTC AAGGTCAAGA CACTCTAGAA 
GCAAAGGTGT CAGTGGAGAA GAGCTCTATT CCAATCCCAA CTATCATTGT TAAAGCCCCT 
TCCACCAGCA GTAGTGGACG CAGTAGCCAA GGCAGCAGTG TGGAGGCTGA ACCACCGCCC 
CCAGGAGAAA GTGACAGTTC TAAACCAGAA CCTGTTGTTC CCCCACCTGC ACCAACCGTG 
CCACCACCAG CCCCTCCATC TATACCTGCT CCTCCACCCC CATCACTGCC ATCACTTCGT 
ACTCAAGAGA GTCTGGACTT CACCAGCCAG TTTGGTGCTG CCATTGTAGG GGCAGCTCGC 
AGGGACAGAG AACGATTTCA TGAAGCACGA CGCAAGAGTG CCTCCTTTTT TCTTTCTGCT 
GAGGAAGAGT TGAGTGGAGC TGGAGGAGCA GGAGGGCGGA CTCAGACTTC TCAACAGCAG 
CTGAGTTCCC AGCCTTCACC ACGCCTGCGA CCATCCAAAT CTATAGATGA AGGCATGTTC 
TCAGGTGATA CTTTTATCCA TCACACACGC AGTATGCCTC CTGCATTTGG CTTGCCTGAG 
TACTCCTCAC CTGCTCCTCA AGGGGATTAC CAGCCTAAGT CTGTACCTGC TGACTTCTAT 
GGGGCAGGAT CCAGACAACA GCAGCAGCAG CAGCAGCAGC AAGCCCCCAC CACAACTTTT 
ATTCACCCAC TAACTGGAAA AGTACTGGAC CCTTCATCCC CTCTAGGCTT GGCTTTGGCT 
GCACGGGAAC GTGCCTTGAA GGATGATGGA CGTATACGTA GGGAAAGGAC CACTGAACAT 
CATTTTACCC GTCAGTTGTC AAGCATTGGG GCCTTTTCCT CATCCACTTC ACAGCCAACA 
TCACACATTT CCTACCCAAT GGCCTCGTCT TCCTCTGCAA CCTCCTCTGC TGCTCAATCC 
ACAAACACCT CTTCATATCT TGTTACCTCC TCATCATTAG CTGCCACCAC CACATCCACT 
CGGCCTCCAT CTCCTAGGAT CCTGCGTGGA GCAGGGAGTA GCTGGAGTGA AGAAGCTGGG 
GTTGGAGAGC GTGCGGATAG GGAAGCAGCT GGCACCTCTG CTCCCAGAGA AGGCCTAAGG 
GTTCGTTTCT CCGAGGATAA AACTGTCCAC ACACACCACT ATCAATCTCA GCAATATCAG 
CCCACCTTTA AAGAGCGTGA AAGAGAACGT CAAAGAGAGA GGTCAAGAGA AAGAGAGAGG 
GAAAGAGAGA GGGAAAGAGA AATAGAAAAA GCTCAAACCC AGTATCAGCA ACAGTCATCA 
CAACCAATGA CCCAGCAGCC TCGACGCCCC TCATTCTTGA GGATGGAGAG TGAAGCTGGA 
GCGTACATTC TTTCATTAAC GCCTCCATCT CCTCCTCCAA CAGCAGCCCC TATGCGTCCT 
ACAGCAGGAG CCACAGGTGA AAGTGGAAGT GGTACAAGCC TGATGGTTCT GCCTCCCCCA 
GCTCCCTCAG TAGATGCAGA TGATGAGTTT GTGTTTGCTG ATCCTCTTCC TCCACCTCTA 
GAGTTTGCCA ATAGTTTTGA TCGTGGTTTG GGTTACTCTG CGATGATTTC TCACAATGTT 
GCAGCCCGTC AGCAGCGTCA TCCTCCACCA CCCCCTCCCC CACCACCCCC GCCCACTTCG 
AAAGATTCCT TTATGACAGG CTTTCCAGCT CACACACCCC TACCGTCACC TCAGGCAGGA 
GATTCCACAA CTTCCAGTCT TACCTCCTAT GACAGTGAGG TTGCAAACCT AACACAATCT 
GCCCCATCGC CCTCCCCATC TTCTCCCAAC CCACTACCAC CTCACTCCCC CTCCACCCTT 
CAGACATCTG GACCACCTCC ACCTCCCTCT GTATCACCTC CTCCACCACC CAACAGCTAC 
CATAGACCTT TTGGCTTATC TCAGTCACAA CATTTATACA ACAGTACACA CACAACTGGA 
CGCTCCTCCT CTCCCACCCC ACCTCTCTCT ATTCCAGCTC CACCTGCCTA CCGGGGGCCA 
CCAACAGCAT CATCCACCCC TGCAACTTCT GTATCAATCA GAGGTGCCTC AACTGTGACC 
GCAAGCTGTT CCACAACTGC TTCTACAACT GTCACCTCTC CTAGTACTAC TGCTGCTGCC 
ACACCACACA CACGCATAGC AACTGTCTCC ACAGTAGCTA CAGGGGGTAG TAGGCGTCCC 
AGTGCAGAGC ATCATCCACC ACCTAGTGCA ACCAAGAGTG GTGAGTCCCA GGAGACAGTG 
GTAGATTCTG GGATTGAGGA GCTGGACAGC CGAAGCAGCA GTGATCATCA CTTAGACAGC 
ATTCTTGCAC TCAGTGGTAG TGGGGTCAGA GAGAGGTTGG AACGAGCAGG GATGAGTAGC 
ATAGGTGGAA TTGGAGGGAG AGATGGTGCA ACTGAGGTTG ACAGGACAAG TGGGGCAGAC 
TTTTTAGAGT CTTACATGAG CTACCTTGAT GGGCAGACTT TTGAAATGCA CAATGCCAAA 
GCCGCTAGCA CTGCATCCAC CTACCCAAAA ACACAGAGGT ACAGAGAAGG CACCCGGGCT 
GGAGATCTTC GACGCCAGAC CAGCACAGCA CCTCCTGCAT ACCACCGTCG TAGAGAGGAG 
GAAGAGGAAG AGGATGAGGC CGAGGAGGTA GAGGAGGAGG ATTTGAGTGC CCGTGATGGC 
TATGGGATGA GGGAAGGGCC AGGGATTGCT CGCTCCTCTG TAATGTACTT TGACCGGCCC 
CGCACTCCAG AGATAAAACC ACTGTGGGGA GATGGGTCGA CTGGAGCATC ACAGACCATG 
GGGGAGGGAG GGGGCCAGAT AGTGGGAACA TATGTGGAGC CACGTAAACT ACATCCTCCC 
ATGACTGGTA TGAAAGCAAA CATCATTAAT GAACTAAGCA CCAAATTACA GCAGAGAAGT 
AAAGGAACAG AGAACTGGGG GGCCCAGAGA TCATTGAGCA GACATAGACA CAGGTTCTCA 
GAGGACTCAA CAGGTGCACT GAGTCCTCCC TCTGTCCGCT CTAGCTCCCC ATCTCCAGTT 
CAGCTCTCTC AGCCTGCCTT GTCTCCCTCT CCCACTCCTT CCTCCCAGCC ACTTTACTCA 
AACTGGGCTC GATCTCCTTC CCCTCAGCCC CCTGCTGCCT CATCCCAGCC CCCTCCCCGA 
TCCCATTCAC CTGTTTCCCC TTCATCATAT TCACCATACC CCACCTCACC CAAACACCGG 
CCTGTTTATC GCACCAAAGC TCTGGAGTTC CAATTTATTC CCAGTCGAGA GTCACGAAAG 
TCAGAGTCGC ATCATGCCCA GCGCAGACGA GCACCCAGCC CTTTAATTTC TCCATCTGAC 
CGTCCCAAGC TTGGCCCTCC CCGCCCCTCT TCCCTTCCTA TTCTCCCCAC TACACCCCTC 
TATAGCAGCC TGTATGACCT TCGTGGTTCT ATTACTCCAC CCTCACCTGC CAATCCCCTT 
GGGGACCCCT ACTTTTCCCC CTCCCCCCCT CTATTTGCCC CCTCGGGTGC TCCCCCTCCT 
CCAAATTCCT CTCTGGTGGT CTCACGGTCC CTGTCACCCA CTCACTTCCT GTCAGGGACT 
TCATCCCCAC CTCTCCACCC CATTCCTCCA CCCACTTGCC TGAGTTACCC CCATCTTCCA 
CCCCCCTCAC CCACAAAGCC CTTTGCCTCA AAACCTCTCC CATACTGGAC CAAGTATGAT 
GTTGCAGATT GGCTGGGCTA CCTGAACCTG GGTGAACACA GGGAGCGATT CTTAGACAAT 
GAAATTGATG GCACGCACCT GCCCTCTCTT ACCAAGGATG ACTACCTGGA CCTGGGTGTG 
ACCCGAGTTG GCCATCGCAT GAACATAGAG AGGGCGCTCA GGAGAGTTAT GGACAGAACC 
ATCAGTAAAG ATCTCATCCC CTCCATCTCC ATATCTTTCA TCTGTTCATC TTCTAAGAGT 
GTGGCATCTT CATTCATCAT ACTGCTCACT TTACCTTACT CATACTGGAC AAAAAGATTG 
AGGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297769.1
CDS	160..5628
Translation	MAMGGCGEEDLSLSLSLSSSWPSLGPMNRSVWFIYSDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMVDRASAPWKKKSEFESSQATEKKRTVYQMALNKLDEILAAAQQTINTNEAPGPRVQGVKRERGRGFYNEPNFDQTGMGMTSSGAGLGYDRGQFVSGHGPPHGMLRQKSIGVPEEEKQFLHPPAMKFARSLSVPGPDEIPPPPTTAPPEPPFSSAPPLGFRGKTSQQPSVSMSQSTSSAAHYQLYSQSVHVTHGPRDRSPGPPTGSGTGDLAYSHAQAHPSVPSRTASRKVIGYTGDLAGAGGVAGAKAAALRRGYSNAVPPTSVATVIPQQQQTTQNQPSRMDRSGMGVGAGGVPKGGARRGKGPLVKQSKVEDLRQGQDTLEAKVSVEKSSIPIPTIIVKAPSTSSSGRSSQGSSVEAEPPPPGESDSSKPEPVVPPPAPTVPPPAPPSIPAPPPPSLPSLRTQESLDFTSQFGAAIVGAARRDRERFHEARRKSASFFLSAEEELSGAGGAGGRTQTSQQQLSSQPSPRLRPSKSIDEGMFSGDTFIHHTRSMPPAFGLPEYSSPAPQGDYQPKSVPADFYGAGSRQQQQQQQQQAPTTTFIHPLTGKVLDPSSPLGLALAARERALKDDGRIRRERTTEHHFTRQLSSIGAFSSSTSQPTSHISYPMASSSSATSSAAQSTNTSSYLVTSSSLAATTTSTRPPSPRILRGAGSSWSEEAGVGERADREAAGTSAPREGLRVRFSEDKTVHTHHYQSQQYQPTFKERERERQRERSREREREREREREIEKAQTQYQQQSSQPMTQQPRRPSFLRMESEAGAYILSLTPPSPPPTAAPMRPTAGATGESGSGTSLMVLPPPAPSVDADDEFVFADPLPPPLEFANSFDRGLGYSAMISHNVAARQQRHPPPPPPPPPPPTSKDSFMTGFPAHTPLPSPQAGDSTTSSLTSYDSEVANLTQSAPSPSPSSPNPLPPHSPSTLQTSGPPPPPSVSPPPPPNSYHRPFGLSQSQHLYNSTHTTGRSSSPTPPLSIPAPPAYRGPPTASSTPATSVSIRGASTVTASCSTTASTTVTSPSTTAAATPHTRIATVSTVATGGSRRPSAEHHPPPSATKSGESQETVVDSGIEELDSRSSSDHHLDSILALSGSGVRERLERAGMSSIGGIGGRDGATEVDRTSGADFLESYMSYLDGQTFEMHNAKAASTASTYPKTQRYREGTRAGDLRRQTSTAPPAYHRRREEEEEEDEAEEVEEEDLSARDGYGMREGPGIARSSVMYFDRPRTPEIKPLWGDGSTGASQTMGEGGGQIVGTYVEPRKLHPPMTGMKANIINELSTKLQQRSKGTENWGAQRSLSRHRHRFSEDSTGALSPPSVRSSSPSPVQLSQPALSPSPTPSSQPLYSNWARSPSPQPPAASSQPPPRSHSPVSPSSYSPYPTSPKHRPVYRTKALEFQFIPSRESRKSESHHAQRRRAPSPLISPSDRPKLGPPRPSSLPILPTTPLYSSLYDLRGSITPPSPANPLGDPYFSPSPPLFAPSGAPPPPNSSLVVSRSLSPTHFLSGTSSPPLHPIPPPTCLSYPHLPPPSPTKPFASKPLPYWTKYDVADWLGYLNLGEHRERFLDNEIDGTHLPSLTKDDYLDLGVTRVGHRMNIERALRRVMDRTISKDLIPSISISFICSSSKSVASSFIILLTLPYSYWTKRLRN

Target ORF information:

RefSeq Version	XM_009299494.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299494.2

ORF Insert Sequence:

ATGGCGATGG GAGGATGCGG AGAGGAGGAT CTCTCTCTCT CCCTCTCCCT CTCGTCGTCC 
TGGCCATCGC TAGGCCCTAT GAATCGCAGC GTCTGGTTCA TTTACAGTGA CTACATAATC 
AAGGAGAAGA CAGTCCTCCT GCAGAAAAAA GACAATGAAG GTTTTGGCTT TGTGCTGAGA 
GGAGCCAAGG CTCAGACCCC TATAGAAGAG TTCACTCCCA CCCCAGCCTT TCCAGCACTG 
CAGTACCTTG AGTCTGTCGA CGAGGGAGGC GTGGCCTGGA GAGCTGGTCT GCGAATGGGA 
GACTTCCTTA TAGAGGTGAA TGGTCAGAAT GTGGTTAAGG TGGGACACAG GCAGGTGGTC 
AATATGATCA GGCAAGGCGG TAACAGTCTC ATGGTCAAGG TTGTCATGGT TACTCGAAAT 
CCTGATATGG AGGAAGGGGT CAGAAAGAAA ATCCCACAGC AGAGTAAAAG ACTGAGCACA 
CCGGCTATCG CTCTGCGCTC CAAATCCATG ACATCAGAGC TGGAAGAGAT GGTGGACAGA 
GCCTCTGCCC CATGGAAAAA AAAATCAGAG TTCGAATCTT CACAAGCTAC AGAGAAGAAG 
AGGACAGTTT ATCAGATGGC TTTGAATAAA CTGGATGAGA TTTTAGCAGC AGCACAGCAG 
ACCATTAATA CAAATGAGGC TCCAGGGCCC CGGGTTCAGG GGGTAAAAAG AGAACGGGGC 
AGAGGCTTTT ATAATGAGCC AAATTTCGAC CAAACTGGAA TGGGGATGAC ATCATCTGGG 
GCAGGGTTAG GCTACGACCG TGGTCAATTT GTGTCTGGCC ATGGCCCCCC GCACGGTATG 
CTGCGACAGA AGTCTATCGG GGTTCCTGAG GAGGAGAAGC AGTTTCTTCA TCCTCCAGCC 
ATGAAGTTTG CCCGTAGTCT TTCAGTACCT GGCCCAGATG AAATCCCTCC TCCACCTACT 
ACAGCACCCC CTGAGCCCCC TTTTTCCTCA GCTCCTCCGC TTGGCTTTAG GGGCAAGACA 
TCTCAACAGC CGTCTGTTTC TATGTCTCAG TCTACTTCCT CTGCTGCACA TTATCAACTC 
TACTCACAGT CAGTACATGT TACTCACGGA CCAAGGGACA GGTCCCCTGG ACCACCTACT 
GGGTCTGGAA CTGGGGACCT TGCTTATTCC CATGCCCAAG CCCACCCCTC TGTACCAAGT 
AGGACTGCCT CCAGGAAGGT GATTGGGTAC ACAGGAGATC TTGCAGGGGC TGGAGGAGTG 
GCTGGGGCCA AAGCTGCAGC ATTGAGAAGG GGTTACAGCA ATGCAGTCCC ACCCACCAGT 
GTCGCTACTG TGATTCCACA ACAGCAGCAG ACTACTCAGA ACCAACCTTC CAGAATGGAC 
CGGAGTGGAA TGGGGGTAGG GGCTGGTGGT GTACCAAAAG GCGGTGCTCG TAGAGGAAAG 
GGGCCCTTGG TGAAACAGTC AAAGGTGGAA GACCTGCGTC AAGGTCAAGA CACTCTAGAA 
GCAAAGGTGT CAGTGGAGAA GAGCTCTATT CCAATCCCAA CTATCATTGT TAAAGCCCCT 
TCCACCAGCA GTAGTGGACG CAGTAGCCAA GGCAGCAGTG TGGAGGCTGA ACCACCGCCC 
CCAGGAGAAA GTGACAGTTC TAAACCAGAA CCTGTTGTTC CCCCACCTGC ACCAACCGTG 
CCACCACCAG CCCCTCCATC TATACCTGCT CCTCCACCCC CATCACTGCC ATCACTTCGT 
ACTCAAGAGA GTCTGGACTT CACCAGCCAG TTTGGTGCTG CCATTGTAGG GGCAGCTCGC 
AGGGACAGAG AACGATTTCA TGAAGCACGA CGCAAGAGTG CCTCCTTTTT TCTTTCTGCT 
GAGGAAGAGT TGAGTGGAGC TGGAGGAGCA GGAGGGCGGA CTCAGACTTC TCAACAGCAG 
CTGAGTTCCC AGCCTTCACC ACGCCTGCGA CCATCCAAAT CTATAGATGA AGGCATGTTC 
TCAGGTGATA CTTTTATCCA TCACACACGC AGTATGCCTC CTGCATTTGG CTTGCCTGAG 
TACTCCTCAC CTGCTCCTCA AGGGGATTAC CAGCCTAAGT CTGTACCTGC TGACTTCTAT 
GGGGCAGGAT CCAGACAACA GCAGCAGCAG CAGCAGCAGC AAGCCCCCAC CACAACTTTT 
ATTCACCCAC TAACTGGAAA AGTACTGGAC CCTTCATCCC CTCTAGGCTT GGCTTTGGCT 
GCACGGGAAC GTGCCTTGAA GGATGATGGA CGTATACGTA GGGAAAGGAC CACTGAACAT 
CATTTTACCC GTCAGTTGTC AAGCATTGGG GCCTTTTCCT CATCCACTTC ACAGCCAACA 
TCACACATTT CCTACCCAAT GGCCTCGTCT TCCTCTGCAA CCTCCTCTGC TGCTCAATCC 
ACAAACACCT CTTCATATCT TGTTACCTCC TCATCATTAG CTGCCACCAC CACATCCACT 
CGGCCTCCAT CTCCTAGGAT CCTGCGTGGA GCAGGGAGTA GCTGGAGTGA AGAAGCTGGG 
GTTGGAGAGC GTGCGGATAG GGAAGCAGCT GGCACCTCTG CTCCCAGAGA AGGCCTAAGG 
GTTCGTTTCT CCGAGGATAA AACTGTCCAC ACACACCACT ATCAATCTCA GCAATATCAG 
CCCACCTTTA AAGAGCGTGA AAGAGAACGT CAAAGAGAGA GGTCAAGAGA AAGAGAGAGG 
GAAAGAGAGA GGGAAAGAGA AATAGAAAAA GCTCAAACCC AGTATCAGCA ACAGTCATCA 
CAACCAATGA CCCAGCAGCC TCGACGCCCC TCATTCTTGA GGATGGAGAG TGAAGCTGGA 
GCGTACATTC TTTCATTAAC GCCTCCATCT CCTCCTCCAA CAGCAGCCCC TATGCGTCCT 
ACAGCAGGAG CCACAGGTGA AAGTGGAAGT GGTACAAGCC TGATGGTTCT GCCTCCCCCA 
GCTCCCTCAG TAGATGCAGA TGATGAGTTT GTGTTTGCTG ATCCTCTTCC TCCACCTCTA 
GAGTTTGCCA ATAGTTTTGA TCGTGGTTTG GGTTACTCTG CGATGATTTC TCACAATGTT 
GCAGCCCGTC AGCAGCGTCA TCCTCCACCA CCCCCTCCCC CACCACCCCC GCCCACTTCG 
AAAGATTCCT TTATGACAGG CTTTCCAGCT CACACACCCC TACCGTCACC TCAGGCAGGA 
GATTCCACAA CTTCCAGTCT TACCTCCTAT GACAGTGAGG TTGCAAACCT AACACAATCT 
GCCCCATCGC CCTCCCCATC TTCTCCCAAC CCACTACCAC CTCACTCCCC CTCCACCCTT 
CAGACATCTG GACCACCTCC ACCTCCCTCT GTATCACCTC CTCCACCACC CAACAGCTAC 
CATAGACCTT TTGGCTTATC TCAGTCACAA CATTTATACA ACAGTACACA CACAACTGGA 
CGCTCCTCCT CTCCCACCCC ACCTCTCTCT ATTCCAGCTC CACCTGCCTA CCGGGGGCCA 
CCAACAGCAT CATCCACCCC TGCAACTTCT GTATCAATCA GAGGTGCCTC AACTGTGACC 
GCAAGCTGTT CCACAACTGC TTCTACAACT GTCACCTCTC CTAGTACTAC TGCTGCTGCC 
ACACCACACA CACGCATAGC AACTGTCTCC ACAGTAGCTA CAGGGGGTAG TAGGCGTCCC 
AGTGCAGAGC ATCATCCACC ACCTAGTGCA ACCAAGAGTG GTGAGTCCCA GGAGACAGTG 
GTAGATTCTG GGATTGAGGA GCTGGACAGC CGAAGCAGCA GTGATCATCA CTTAGACAGC 
ATTCTTGCAC TCAGTGGTAG TGGGGTCAGA GAGAGGTTGG AACGAGCAGG GATGAGTAGC 
ATAGGTGGAA TTGGAGGGAG AGATGGTGCA ACTGAGGTTG ACAGGACAAG TGGGGCAGAC 
TTTTTAGAGT CTTACATGAG CTACCTTGAT GGGCAGACTT TTGAAATGCA CAATGCCAAA 
GCCGCTAGCA CTGCATCCAC CTACCCAAAA ACACAGAGGT ACAGAGAAGG CACCCGGGCT 
GGAGATCTTC GACGCCAGAC CAGCACAGCA CCTCCTGCAT ACCACCGTCG TAGAGAGGAG 
GAAGAGGAAG AGGATGAGGC CGAGGAGGTA GAGGAGGAGG ATTTGAGTGC CCGTGATGGC 
TATGGGATGA GGGAAGGGCC AGGGATTGCT CGCTCCTCTG TAATGTACTT TGACCGGCCC 
CGCACTCCAG AGATAAAACC ACTGTGGGGA GATGGGTCGA CTGGAGCATC ACAGACCATG 
GGGGAGGGAG GGGGCCAGAT AGTGGGAACA TATGTGGAGC CACGTAAACT ACATCCTCCC 
ATGACTGGTA TGAAAGCAAA CATCATTAAT GAACTAAGCA CCAAATTACA GCAGAGAAGT 
AAAGGAACAG AGAACTGGGG GGCCCAGAGA TCATTGAGCA GACATAGACA CAGGTTCTCA 
GAGGACTCAA CAGGTGCACT GAGTCCTCCC TCTGTCCGCT CTAGCTCCCC ATCTCCAGTT 
CAGCTCTCTC AGCCTGCCTT GTCTCCCTCT CCCACTCCTT CCTCCCAGCC ACTTTACTCA 
AACTGGGCTC GATCTCCTTC CCCTCAGCCC CCTGCTGCCT CATCCCAGCC CCCTCCCCGA 
TCCCATTCAC CTGTTTCCCC TTCATCATAT TCACCATACC CCACCTCACC CAAACACCGG 
CCTGTTTATC GCACCAAAGC TCTGGAGTTC CAATTTATTC CCAGTCGAGA GTCACGAAAG 
TCAGAGTCGC ATCATGCCCA GCGCAGACGA GCACCCAGCC CTTTAATTTC TCCATCTGAC 
CGTCCCAAGC TTGGCCCTCC CCGCCCCTCT TCCCTTCCTA TTCTCCCCAC TACACCCCTC 
TATAGCAGCC TGTATGACCT TCGTGGTTCT ATTACTCCAC CCTCACCTGC CAATCCCCTT 
GGGGACCCCT ACTTTTCCCC CTCCCCCCCT CTATTTGCCC CCTCGGGTGC TCCCCCTCCT 
CCAAATTCCT CTCTGGTGGT CTCACGGTCC CTGTCACCCA CTCACTTCCT GTCAGGGACT 
TCATCCCCAC CTCTCCACCC CATTCCTCCA CCCACTTGCC TGAGTTACCC CCATCTTCCA 
CCCCCCTCAC CCACAAAGCC CTTTGCCTCA AAACCTCTCC CATACTGGAC CAAGTATGAT 
GTTGCAGATT GGCTGGGCTA CCTGAACCTG GGTGAACACA GGGAGCGATT CTTAGACAAT 
GAAATTGATG GCACGCACCT GCCCTCTCTT ACCAAGGATG ACTACCTGGA CCTGGGTGTG 
ACCCGAGTTG GCCATCGCAT GAACATAGAG AGGGCGCTCA GGAGAGTTAT GGACAGAACC 
ATCAGTAAAG ATCTCATCCC CTCCATCTCC ATATCTTTCA TCTGTTCATC TTCTAAGAGT 
GTGGCATCTT CATTCATCAT ACTGCTCACT TTACCTTACT CATACTGGAC AAAAAGATTG 
AGGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_009299495.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297770.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X10, mRNA.

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CloneID	ODa45598
Clone ID Related Accession (Same CDS sequence)	XM_009299495.2
Accession Version	XM_009299495.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299495.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGACT ACATAATCAA GGAGAAGACA GTCCTCCTGC AGAAAAAAGA CAATGAAGGT 
TTTGGCTTTG TGCTGAGAGG AGCCAAGGCT CAGACCCCTA TAGAAGAGTT CACTCCCACC 
CCAGCCTTTC CAGCACTGCA GTACCTTGAG TCTGTCGACG AGGGAGGCGT GGCCTGGAGA 
GCTGGTCTGC GAATGGGAGA CTTCCTTATA GAGGTGAATG GTCAGAATGT GGTTAAGGTG 
GGACACAGGC AGGTGGTCAA TATGATCAGG CAAGGCGGTA ACAGTCTCAT GGTCAAGGTT 
GTCATGGTTA CTCGAAATCC TGATATGGAG GAAGGGGTCA GAAAGAAAAT CCCACAGCAG 
AGTAAAAGAC TGAGCACACC GGCTATCGCT CTGCGCTCCA AATCCATGAC ATCAGAGCTG 
GAAGAGATGG TGGACAGAGC CTCTGCCCCA TGGAAAAAAA AATCAGAGTT CGAATCTTCA 
CAAGCTACAG AGAAGAAGAG GACAGTTTAT CAGATGGCTT TGAATAAACT GGATGAGATT 
TTAGCAGCAG CACAGCAGAC CATTAATACA AATGAGGCTC CAGGGCCCCG GGTTCAGGGG 
GTAAAAAGAG AACGGGGCAG AGGCTTTTAT AATGAGCCAA ATTTCGACCA AACTGGAATG 
GGGATGACAT CATCTGGGGC AGGGTTAGGC TACGACCGTG GTCAATTTGT GTCTGGCCAT 
GGCCCCCCGC ACGGTATGCT GCGACAGAAG TCTATCGGGG TTCCTGAGGA GGAGAAGCAG 
TTTCTTCATC CTCCAGCCAT GAAGTTTGCC CGTAGTCTTT CAGTACCTGG CCCAGATGAA 
ATCCCTCCTC CACCTACTAC AGCACCCCCT GAGCCCCCTT TTTCCTCAGC TCCTCCGCTT 
GGCTTTAGGG GCAAGACATC TCAACAGCCG TCTGTTTCTA TGTCTCAGTC TACTTCCTCT 
GCTGCACATT ATCAACTCTA CTCACAGTCA GTACATGTTA CTCACGGACC AAGGGACAGG 
TCCCCTGGAC CACCTACTGG GTCTGGAACT GGGGACCTTG CTTATTCCCA TGCCCAAGCC 
CACCCCTCTG TACCAAGTAG GACTGCCTCC AGGAAGGTGA TTGGGTACAC AGGAGATCTT 
GCAGGGGCTG GAGGAGTGGC TGGGGCCAAA GCTGCAGCAT TGAGAAGGGG TTACAGCAAT 
GCAGTCCCAC CCACCAGTGT CGCTACTGTG ATTCCACAAC AGCAGCAGAC TACTCAGAAC 
CAACCTTCCA GAATGGACCG GAGTGGAATG GGGGTAGGGG CTGGTGGTGT ACCAAAAGGC 
GGTGCTCGTA GAGGAAAGGG GCCCTTGGTG AAACAGTCAA AGGTGGAAGA CCTGCGTCAA 
GGTCAAGACA CTCTAGAAGC AAAGGTGTCA GTGGAGAAGA GCTCTATTCC AATCCCAACT 
ATCATTGTTA AAGCCCCTTC CACCAGCAGT AGTGGACGCA GTAGCCAAGG CAGCAGTGTG 
GAGGCTGAAC CACCGCCCCC AGGAGAAAGT GACAGTTCTA AACCAGAACC TGTTGTTCCC 
CCACCTGCAC CAACCGTGCC ACCACCAGCC CCTCCATCTA TACCTGCTCC TCCACCCCCA 
TCACTGCCAT CACTTCGTAC TCAAGAGAGT CTGGACTTCA CCAGCCAGTT TGGTGCTGCC 
ATTGTAGGGG CAGCTCGCAG GGACAGAGAA CGATTTCATG AAGCACGACG CAAGAGTGCC 
TCCTTTTTTC TTTCTGCTGA GGAAGAGTTG AGTGGAGCTG GAGGAGCAGG AGGGCGGACT 
CAGACTTCTC AACAGCAGCT GAGTTCCCAG CCTTCACCAC GCCTGCGACC ATCCAAATCT 
ATAGATGAAG GCATGTTCTC AGGTGATACT TTTATCCATC ACACACGCAG TATGCCTCCT 
GCATTTGGCT TGCCTGAGTA CTCCTCACCT GCTCCTCAAG GGGATTACCA GCCTAAGTCT 
GTACCTGCTG ACTTCTATGG GGCAGGATCC AGACAACAGC AGCAGCAGCA GCAGCAGCAA 
GCCCCCACCA CAACTTTTAT TCACCCACTA ACTGGAAAAG TACTGGACCC TTCATCCCCT 
CTAGGCTTGG CTTTGGCTGC ACGGGAACGT GCCTTGAAGG ATGATGGACG TATACGTAGG 
GAAAGGACCA CTGAACATCA TTTTACCCGT CAGTTGTCAA GCATTGGGGC CTTTTCCTCA 
TCCACTTCAC AGCCAACATC ACACATTTCC TACCCAATGG CCTCGTCTTC CTCTGCAACC 
TCCTCTGCTG CTCAATCCAC AAACACCTCT TCATATCTTG TTACCTCCTC ATCATTAGCT 
GCCACCACCA CATCCACTCG GCCTCCATCT CCTAGGATCC TGCGTGGAGC AGGGAGTAGC 
TGGAGTGAAG AAGCTGGGGT TGGAGAGCGT GCGGATAGGG AAGCAGCTGG CACCTCTGCT 
CCCAGAGAAG GCCTAAGGGT TCGTTTCTCC GAGGATAAAA CTGTCCACAC ACACCACTAT 
CAATCTCAGC AATATCAGCC CACCTTTAAA GAGCGTGAAA GAGAACGTCA AAGAGAGAGG 
TCAAGAGAAA GAGAGAGGGA AAGAGAGAGG GAAAGAGAAA TAGAAAAAGC TCAAACCCAG 
TATCAGCAAC AGTCATCACA ACCAATGACC CAGCAGCCTC GACGCCCCTC ATTCTTGAGG 
ATGGAGAGTG AAGCTGGAGC GTACATTCTT TCATTAACGC CTCCATCTCC TCCTCCAACA 
GCAGCCCCTA TGCGTCCTAC AGCAGGAGCC ACAGGTGAAA GTGGAAGTGG TACAAGCCTG 
ATGGTTCTGC CTCCCCCAGC TCCCTCAGTA GATGCAGATG ATGAGTTTGT GTTTGCTGAT 
CCTCTTCCTC CACCTCTAGA GTTTGCCAAT AGTTTTGATC GTGGTTTGGG TTACTCTGCG 
ATGATTTCTC ACAATGTTGC AGCCCGTCAG CAGCGTCATC CTCCACCACC CCCTCCCCCA 
CCACCCCCGC CCACTTCGAA AGATTCCTTT ATGACAGGCT TTCCAGCTCA CACACCCCTA 
CCGTCACCTC AGGCAGGAGA TTCCACAACT TCCAGTCTTA CCTCCTATGA CAGTGAGGTT 
GCAAACCTAA CACAATCTGC CCCATCGCCC TCCCCATCTT CTCCCAACCC ACTACCACCT 
CACTCCCCCT CCACCCTTCA GACATCTGGA CCACCTCCAC CTCCCTCTGT ATCACCTCCT 
CCACCACCCA ACAGCTACCA TAGACCTTTT GGCTTATCTC AGTCACAACA TTTATACAAC 
AGTACACACA CAACTGGACG CTCCTCCTCT CCCACCCCAC CTCTCTCTAT TCCAGCTCCA 
CCTGCCTACC GGGGGCCACC AACAGCATCA TCCACCCCTG CAACTTCTGT ATCAATCAGA 
GGTGCCTCAA CTGTGACCGC AAGCTGTTCC ACAACTGCTT CTACAACTGT CACCTCTCCT 
AGTACTACTG CTGCTGCCAC ACCACACACA CGCATAGCAA CTGTCTCCAC AGTAGCTACA 
GGGGGTAGTA GGCGTCCCAG TGCAGAGCAT CATCCACCAC CTAGTGCAAC CAAGAGTGGT 
GAGTCCCAGG AGACAGTGGT AGATTCTGGG ATTGAGGAGC TGGACAGCCG AAGCAGCAGT 
GATCATCACT TAGACAGCAT TCTTGCACTC AGTGGTAGTG GGGTCAGAGA GAGGTTGGAA 
CGAGCAGGGA TGAGTAGCAT AGGTGGAATT GGAGGGAGAG ATGGTGCAAC TGAGGTTGAC 
AGGACAAGTG GGGCAGACTT TTTAGAGTCT TACATGAGCT ACCTTGATGG GCAGACTTTT 
GAAATGCACA ATGCCAAAGC CGCTAGCACT GCATCCACCT ACCCAAAAAC ACAGAGGTAC 
AGAGAAGGCA CCCGGGCTGG AGATCTTCGA CGCCAGACCA GCACAGCACC TCCTGCATAC 
CACCGTCGTA GAGAGGAGGA AGAGGAAGAG GATGAGGCCG AGGAGGTAGA GGAGGAGGAT 
TTGAGTGCCC GTGATGGCTA TGGGATGAGG GAAGGGCCAG GGATTGCTCG CTCCTCTGTA 
ATGTACTTTG ACCGGCCCCG CACTCCAGAG ATAAAACCAC TGTGGGGAGA TGGGTCGACT 
GGAGCATCAC AGACCATGGG GGAGGGAGGG GGCCAGATAG TGGGAACATA TGTGGAGCCA 
CGTAAACTAC ATCCTCCCAT GACTGGTATG AAAGCAAACA TCATTAATGA ACTAAGCACC 
AAATTACAGC AGAGAAGTAA AGGAACAGAG AACTGGGGGG CCCAGAGATC ATTGAGCAGA 
CATAGACACA GGTTCTCAGA GGACTCAACA GGTGCACTGA GTCCTCCCTC TGTCCGCTCT 
AGCTCCCCAT CTCCAGTTCA GCTCTCTCAG CCTGCCTTGT CTCCCTCTCC CACTCCTTCC 
TCCCAGCCAC TTTACTCAAA CTGGGCTCGA TCTCCTTCCC CTCAGCCCCC TGCTGCCTCA 
TCCCAGCCCC CTCCCCGATC CCATTCACCT GTTTCCCCTT CATCATATTC ACCATACCCC 
ACCTCACCCA AACACCGGCC TGTTTATCGC ACCAAAGCTC TGGAGTTCCA ATTTATTCCC 
AGTCGAGAGT CACGAAAGTC AGAGTCGCAT CATGCCCAGC GCAGACGAGC ACCCAGCCCT 
TTAATTTCTC CATCTGACCG TCCCAAGCTT GGCCCTCCCC GCCCCTCTTC CCTTCCTATT 
CTCCCCACTA CACCCCTCTA TAGCAGCCTG TATGACCTTC GTGGTTCTAT TACTCCACCC 
TCACCTGCCA ATCCCCTTGG GGACCCCTAC TTTTCCCCCT CCCCCCCTCT ATTTGCCCCC 
TCGGGTGCTC CCCCTCCTCC AAATTCCTCT CTGGTGGTCT CACGGTCCCT GTCACCCACT 
CACTTCCTGT CAGGGACTTC ATCCCCACCT CTCCACCCCA TTCCTCCACC CACTTGCCTG 
AGTTACCCCC ATCTTCCACC CCCCTCACCC ACAAAGCCCT TTGCCTCAAA ACCTCTCCCA 
TACTGGACCA AGTATGATGT TGCAGATTGG CTGGGCTACC TGAACCTGGG TGAACACAGG 
GAGCGATTCT TAGACAATGA AATTGATGGC ACGCACCTGC CCTCTCTTAC CAAGGATGAC 
TACCTGGACC TGGGTGTGAC CCGAGTTGGC CATCGCATGA ACATAGAGAG GGCGCTCAGG 
AGAGTTATGG ACAGAACCAT CAGTAAAGAT CTCATCCCCT CCATCTCCAT ATCTTTCATC 
TGTTCATCTT CTAAGAGTGT GGCATCTTCA TTCATCATAC TGCTCACTTT ACCTTACTCA 
TACTGGACAA AAAGATTGAG GAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297770.1
CDS	238..5604
Translation	MIDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMVDRASAPWKKKSEFESSQATEKKRTVYQMALNKLDEILAAAQQTINTNEAPGPRVQGVKRERGRGFYNEPNFDQTGMGMTSSGAGLGYDRGQFVSGHGPPHGMLRQKSIGVPEEEKQFLHPPAMKFARSLSVPGPDEIPPPPTTAPPEPPFSSAPPLGFRGKTSQQPSVSMSQSTSSAAHYQLYSQSVHVTHGPRDRSPGPPTGSGTGDLAYSHAQAHPSVPSRTASRKVIGYTGDLAGAGGVAGAKAAALRRGYSNAVPPTSVATVIPQQQQTTQNQPSRMDRSGMGVGAGGVPKGGARRGKGPLVKQSKVEDLRQGQDTLEAKVSVEKSSIPIPTIIVKAPSTSSSGRSSQGSSVEAEPPPPGESDSSKPEPVVPPPAPTVPPPAPPSIPAPPPPSLPSLRTQESLDFTSQFGAAIVGAARRDRERFHEARRKSASFFLSAEEELSGAGGAGGRTQTSQQQLSSQPSPRLRPSKSIDEGMFSGDTFIHHTRSMPPAFGLPEYSSPAPQGDYQPKSVPADFYGAGSRQQQQQQQQQAPTTTFIHPLTGKVLDPSSPLGLALAARERALKDDGRIRRERTTEHHFTRQLSSIGAFSSSTSQPTSHISYPMASSSSATSSAAQSTNTSSYLVTSSSLAATTTSTRPPSPRILRGAGSSWSEEAGVGERADREAAGTSAPREGLRVRFSEDKTVHTHHYQSQQYQPTFKERERERQRERSREREREREREREIEKAQTQYQQQSSQPMTQQPRRPSFLRMESEAGAYILSLTPPSPPPTAAPMRPTAGATGESGSGTSLMVLPPPAPSVDADDEFVFADPLPPPLEFANSFDRGLGYSAMISHNVAARQQRHPPPPPPPPPPPTSKDSFMTGFPAHTPLPSPQAGDSTTSSLTSYDSEVANLTQSAPSPSPSSPNPLPPHSPSTLQTSGPPPPPSVSPPPPPNSYHRPFGLSQSQHLYNSTHTTGRSSSPTPPLSIPAPPAYRGPPTASSTPATSVSIRGASTVTASCSTTASTTVTSPSTTAAATPHTRIATVSTVATGGSRRPSAEHHPPPSATKSGESQETVVDSGIEELDSRSSSDHHLDSILALSGSGVRERLERAGMSSIGGIGGRDGATEVDRTSGADFLESYMSYLDGQTFEMHNAKAASTASTYPKTQRYREGTRAGDLRRQTSTAPPAYHRRREEEEEEDEAEEVEEEDLSARDGYGMREGPGIARSSVMYFDRPRTPEIKPLWGDGSTGASQTMGEGGGQIVGTYVEPRKLHPPMTGMKANIINELSTKLQQRSKGTENWGAQRSLSRHRHRFSEDSTGALSPPSVRSSSPSPVQLSQPALSPSPTPSSQPLYSNWARSPSPQPPAASSQPPPRSHSPVSPSSYSPYPTSPKHRPVYRTKALEFQFIPSRESRKSESHHAQRRRAPSPLISPSDRPKLGPPRPSSLPILPTTPLYSSLYDLRGSITPPSPANPLGDPYFSPSPPLFAPSGAPPPPNSSLVVSRSLSPTHFLSGTSSPPLHPIPPPTCLSYPHLPPPSPTKPFASKPLPYWTKYDVADWLGYLNLGEHRERFLDNEIDGTHLPSLTKDDYLDLGVTRVGHRMNIERALRRVMDRTISKDLIPSISISFICSSSKSVASSFIILLTLPYSYWTKRLRN

Target ORF information:

RefSeq Version	XM_009299495.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299495.2

ORF Insert Sequence:

ATGATTGACT ACATAATCAA GGAGAAGACA GTCCTCCTGC AGAAAAAAGA CAATGAAGGT 
TTTGGCTTTG TGCTGAGAGG AGCCAAGGCT CAGACCCCTA TAGAAGAGTT CACTCCCACC 
CCAGCCTTTC CAGCACTGCA GTACCTTGAG TCTGTCGACG AGGGAGGCGT GGCCTGGAGA 
GCTGGTCTGC GAATGGGAGA CTTCCTTATA GAGGTGAATG GTCAGAATGT GGTTAAGGTG 
GGACACAGGC AGGTGGTCAA TATGATCAGG CAAGGCGGTA ACAGTCTCAT GGTCAAGGTT 
GTCATGGTTA CTCGAAATCC TGATATGGAG GAAGGGGTCA GAAAGAAAAT CCCACAGCAG 
AGTAAAAGAC TGAGCACACC GGCTATCGCT CTGCGCTCCA AATCCATGAC ATCAGAGCTG 
GAAGAGATGG TGGACAGAGC CTCTGCCCCA TGGAAAAAAA AATCAGAGTT CGAATCTTCA 
CAAGCTACAG AGAAGAAGAG GACAGTTTAT CAGATGGCTT TGAATAAACT GGATGAGATT 
TTAGCAGCAG CACAGCAGAC CATTAATACA AATGAGGCTC CAGGGCCCCG GGTTCAGGGG 
GTAAAAAGAG AACGGGGCAG AGGCTTTTAT AATGAGCCAA ATTTCGACCA AACTGGAATG 
GGGATGACAT CATCTGGGGC AGGGTTAGGC TACGACCGTG GTCAATTTGT GTCTGGCCAT 
GGCCCCCCGC ACGGTATGCT GCGACAGAAG TCTATCGGGG TTCCTGAGGA GGAGAAGCAG 
TTTCTTCATC CTCCAGCCAT GAAGTTTGCC CGTAGTCTTT CAGTACCTGG CCCAGATGAA 
ATCCCTCCTC CACCTACTAC AGCACCCCCT GAGCCCCCTT TTTCCTCAGC TCCTCCGCTT 
GGCTTTAGGG GCAAGACATC TCAACAGCCG TCTGTTTCTA TGTCTCAGTC TACTTCCTCT 
GCTGCACATT ATCAACTCTA CTCACAGTCA GTACATGTTA CTCACGGACC AAGGGACAGG 
TCCCCTGGAC CACCTACTGG GTCTGGAACT GGGGACCTTG CTTATTCCCA TGCCCAAGCC 
CACCCCTCTG TACCAAGTAG GACTGCCTCC AGGAAGGTGA TTGGGTACAC AGGAGATCTT 
GCAGGGGCTG GAGGAGTGGC TGGGGCCAAA GCTGCAGCAT TGAGAAGGGG TTACAGCAAT 
GCAGTCCCAC CCACCAGTGT CGCTACTGTG ATTCCACAAC AGCAGCAGAC TACTCAGAAC 
CAACCTTCCA GAATGGACCG GAGTGGAATG GGGGTAGGGG CTGGTGGTGT ACCAAAAGGC 
GGTGCTCGTA GAGGAAAGGG GCCCTTGGTG AAACAGTCAA AGGTGGAAGA CCTGCGTCAA 
GGTCAAGACA CTCTAGAAGC AAAGGTGTCA GTGGAGAAGA GCTCTATTCC AATCCCAACT 
ATCATTGTTA AAGCCCCTTC CACCAGCAGT AGTGGACGCA GTAGCCAAGG CAGCAGTGTG 
GAGGCTGAAC CACCGCCCCC AGGAGAAAGT GACAGTTCTA AACCAGAACC TGTTGTTCCC 
CCACCTGCAC CAACCGTGCC ACCACCAGCC CCTCCATCTA TACCTGCTCC TCCACCCCCA 
TCACTGCCAT CACTTCGTAC TCAAGAGAGT CTGGACTTCA CCAGCCAGTT TGGTGCTGCC 
ATTGTAGGGG CAGCTCGCAG GGACAGAGAA CGATTTCATG AAGCACGACG CAAGAGTGCC 
TCCTTTTTTC TTTCTGCTGA GGAAGAGTTG AGTGGAGCTG GAGGAGCAGG AGGGCGGACT 
CAGACTTCTC AACAGCAGCT GAGTTCCCAG CCTTCACCAC GCCTGCGACC ATCCAAATCT 
ATAGATGAAG GCATGTTCTC AGGTGATACT TTTATCCATC ACACACGCAG TATGCCTCCT 
GCATTTGGCT TGCCTGAGTA CTCCTCACCT GCTCCTCAAG GGGATTACCA GCCTAAGTCT 
GTACCTGCTG ACTTCTATGG GGCAGGATCC AGACAACAGC AGCAGCAGCA GCAGCAGCAA 
GCCCCCACCA CAACTTTTAT TCACCCACTA ACTGGAAAAG TACTGGACCC TTCATCCCCT 
CTAGGCTTGG CTTTGGCTGC ACGGGAACGT GCCTTGAAGG ATGATGGACG TATACGTAGG 
GAAAGGACCA CTGAACATCA TTTTACCCGT CAGTTGTCAA GCATTGGGGC CTTTTCCTCA 
TCCACTTCAC AGCCAACATC ACACATTTCC TACCCAATGG CCTCGTCTTC CTCTGCAACC 
TCCTCTGCTG CTCAATCCAC AAACACCTCT TCATATCTTG TTACCTCCTC ATCATTAGCT 
GCCACCACCA CATCCACTCG GCCTCCATCT CCTAGGATCC TGCGTGGAGC AGGGAGTAGC 
TGGAGTGAAG AAGCTGGGGT TGGAGAGCGT GCGGATAGGG AAGCAGCTGG CACCTCTGCT 
CCCAGAGAAG GCCTAAGGGT TCGTTTCTCC GAGGATAAAA CTGTCCACAC ACACCACTAT 
CAATCTCAGC AATATCAGCC CACCTTTAAA GAGCGTGAAA GAGAACGTCA AAGAGAGAGG 
TCAAGAGAAA GAGAGAGGGA AAGAGAGAGG GAAAGAGAAA TAGAAAAAGC TCAAACCCAG 
TATCAGCAAC AGTCATCACA ACCAATGACC CAGCAGCCTC GACGCCCCTC ATTCTTGAGG 
ATGGAGAGTG AAGCTGGAGC GTACATTCTT TCATTAACGC CTCCATCTCC TCCTCCAACA 
GCAGCCCCTA TGCGTCCTAC AGCAGGAGCC ACAGGTGAAA GTGGAAGTGG TACAAGCCTG 
ATGGTTCTGC CTCCCCCAGC TCCCTCAGTA GATGCAGATG ATGAGTTTGT GTTTGCTGAT 
CCTCTTCCTC CACCTCTAGA GTTTGCCAAT AGTTTTGATC GTGGTTTGGG TTACTCTGCG 
ATGATTTCTC ACAATGTTGC AGCCCGTCAG CAGCGTCATC CTCCACCACC CCCTCCCCCA 
CCACCCCCGC CCACTTCGAA AGATTCCTTT ATGACAGGCT TTCCAGCTCA CACACCCCTA 
CCGTCACCTC AGGCAGGAGA TTCCACAACT TCCAGTCTTA CCTCCTATGA CAGTGAGGTT 
GCAAACCTAA CACAATCTGC CCCATCGCCC TCCCCATCTT CTCCCAACCC ACTACCACCT 
CACTCCCCCT CCACCCTTCA GACATCTGGA CCACCTCCAC CTCCCTCTGT ATCACCTCCT 
CCACCACCCA ACAGCTACCA TAGACCTTTT GGCTTATCTC AGTCACAACA TTTATACAAC 
AGTACACACA CAACTGGACG CTCCTCCTCT CCCACCCCAC CTCTCTCTAT TCCAGCTCCA 
CCTGCCTACC GGGGGCCACC AACAGCATCA TCCACCCCTG CAACTTCTGT ATCAATCAGA 
GGTGCCTCAA CTGTGACCGC AAGCTGTTCC ACAACTGCTT CTACAACTGT CACCTCTCCT 
AGTACTACTG CTGCTGCCAC ACCACACACA CGCATAGCAA CTGTCTCCAC AGTAGCTACA 
GGGGGTAGTA GGCGTCCCAG TGCAGAGCAT CATCCACCAC CTAGTGCAAC CAAGAGTGGT 
GAGTCCCAGG AGACAGTGGT AGATTCTGGG ATTGAGGAGC TGGACAGCCG AAGCAGCAGT 
GATCATCACT TAGACAGCAT TCTTGCACTC AGTGGTAGTG GGGTCAGAGA GAGGTTGGAA 
CGAGCAGGGA TGAGTAGCAT AGGTGGAATT GGAGGGAGAG ATGGTGCAAC TGAGGTTGAC 
AGGACAAGTG GGGCAGACTT TTTAGAGTCT TACATGAGCT ACCTTGATGG GCAGACTTTT 
GAAATGCACA ATGCCAAAGC CGCTAGCACT GCATCCACCT ACCCAAAAAC ACAGAGGTAC 
AGAGAAGGCA CCCGGGCTGG AGATCTTCGA CGCCAGACCA GCACAGCACC TCCTGCATAC 
CACCGTCGTA GAGAGGAGGA AGAGGAAGAG GATGAGGCCG AGGAGGTAGA GGAGGAGGAT 
TTGAGTGCCC GTGATGGCTA TGGGATGAGG GAAGGGCCAG GGATTGCTCG CTCCTCTGTA 
ATGTACTTTG ACCGGCCCCG CACTCCAGAG ATAAAACCAC TGTGGGGAGA TGGGTCGACT 
GGAGCATCAC AGACCATGGG GGAGGGAGGG GGCCAGATAG TGGGAACATA TGTGGAGCCA 
CGTAAACTAC ATCCTCCCAT GACTGGTATG AAAGCAAACA TCATTAATGA ACTAAGCACC 
AAATTACAGC AGAGAAGTAA AGGAACAGAG AACTGGGGGG CCCAGAGATC ATTGAGCAGA 
CATAGACACA GGTTCTCAGA GGACTCAACA GGTGCACTGA GTCCTCCCTC TGTCCGCTCT 
AGCTCCCCAT CTCCAGTTCA GCTCTCTCAG CCTGCCTTGT CTCCCTCTCC CACTCCTTCC 
TCCCAGCCAC TTTACTCAAA CTGGGCTCGA TCTCCTTCCC CTCAGCCCCC TGCTGCCTCA 
TCCCAGCCCC CTCCCCGATC CCATTCACCT GTTTCCCCTT CATCATATTC ACCATACCCC 
ACCTCACCCA AACACCGGCC TGTTTATCGC ACCAAAGCTC TGGAGTTCCA ATTTATTCCC 
AGTCGAGAGT CACGAAAGTC AGAGTCGCAT CATGCCCAGC GCAGACGAGC ACCCAGCCCT 
TTAATTTCTC CATCTGACCG TCCCAAGCTT GGCCCTCCCC GCCCCTCTTC CCTTCCTATT 
CTCCCCACTA CACCCCTCTA TAGCAGCCTG TATGACCTTC GTGGTTCTAT TACTCCACCC 
TCACCTGCCA ATCCCCTTGG GGACCCCTAC TTTTCCCCCT CCCCCCCTCT ATTTGCCCCC 
TCGGGTGCTC CCCCTCCTCC AAATTCCTCT CTGGTGGTCT CACGGTCCCT GTCACCCACT 
CACTTCCTGT CAGGGACTTC ATCCCCACCT CTCCACCCCA TTCCTCCACC CACTTGCCTG 
AGTTACCCCC ATCTTCCACC CCCCTCACCC ACAAAGCCCT TTGCCTCAAA ACCTCTCCCA 
TACTGGACCA AGTATGATGT TGCAGATTGG CTGGGCTACC TGAACCTGGG TGAACACAGG 
GAGCGATTCT TAGACAATGA AATTGATGGC ACGCACCTGC CCTCTCTTAC CAAGGATGAC 
TACCTGGACC TGGGTGTGAC CCGAGTTGGC CATCGCATGA ACATAGAGAG GGCGCTCAGG 
AGAGTTATGG ACAGAACCAT CAGTAAAGAT CTCATCCCCT CCATCTCCAT ATCTTTCATC 
TGTTCATCTT CTAAGAGTGT GGCATCTTCA TTCATCATAC TGCTCACTTT ACCTTACTCA 
TACTGGACAA AAAGATTGAG GAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_021474080.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329755.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X6, mRNA.

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CloneID	ODa141768
Clone ID Related Accession (Same CDS sequence)	XM_021474080.1 , XM_021471436.1
Accession Version	XM_021474080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATGG GAGGATGCGG AGAGGAGGAT CTCTCTCTCT CCCTCTCCCT CTCGTCGTCC 
TGGCCATCGC TAGGCCCTAT GAATCGCAGC GTCTGGTTCA TTTACAGTGA CTACATAATC 
AAGGAGAAGA CAGTCCTCCT GCAGAAAAAA GACAATGAAG GTTTTGGCTT TGTGCTGAGA 
GGAGCCAAGG CTCAGACCCC TATAGAAGAG TTCACTCCCA CCCCAGCCTT TCCAGCACTG 
CAGTACCTTG AGTCTGTCGA CGAGGGAGGC GTGGCCTGGA GAGCTGGTCT GCGAATGGGA 
GACTTCCTTA TAGAGGTGAA TGGTCAGAAT GTGGTTAAGG TGGGACACAG GCAGGTGGTC 
AATATGATCA GGCAAGGCGG TAACAGTCTC ATGGTCAAGG TTGTCATGGT TACTCGAAAT 
CCTGATATGG AGGAAGGGGT CAGAAAGAAA ATCCCACAGC AGAGTAAAAG ACTGAGCACA 
CCGGCTATCG CTCTGCGCTC CAAATCCATG ACATCAGAGC TGGAAGAGAT GGTGGACAGA 
GCCTCTGCCC CATGGAAAAA AAAATCAGAG TTCGAATCTT CACAAGCTAC AGAGAAGAAG 
AGGACAGTTT ATCAGATGGC TTTGAATAAA CTGGATGAGA TTTTAGCAGC AGCACAGCAG 
ACCATTAATA CAAATGAGGC TCCAGGGCCC CGGGTTCAGG GGGTAAAAAG AGAACGGGGC 
AGAGGCTTTT ATAATGAGCC AAATTTCGAC CAAACTGGAA TGGGGATGAC ATCATCTGGG 
GCAGGGTTAG GCTACGACCG TGGTCAATTT GTGTCTGGCC ATGGCCCCCC GCACGGTATG 
CTGCGACAGA AGTCTATCGG GGTTCCTGAG GAGGAGAAGC AGTTTCTTCA TCCTCCAGCC 
ATGAAGTTTG CCCGTAGTCT TTCAGTACCT GGCCCAGATG AAATCCCTCC TCCACCTACT 
ACAGCACCCC CTGAGCCCCC TTTTTCCTCA GCTCCTCCGC TTGGCTTTAG GGGCAAGACA 
TCTCAACAGC CGTCTGTTTC TATGTCTCAG TCTACTTCCT CTGCTGCACA TTATCAACTC 
TACTCACAGT CAGTACATGT TACTCACGGA CCAAGGGACA GGTCCCCTGG ACCACCTACT 
GGGTCTGGAA CTGGGGACCT TGCTTATTCC CATGCCCAAG CCCACCCCTC TGTACCAAGT 
AGGACTGCCT CCAGGAAGGT GATTGGGTAC ACAGGAGATC TTGCAGGGGC TGGAGGAGTG 
GCTGGGGCCA AAGCTGCAGC ATTGAGAAGG GGTTACAGCA ATGCAGTCCC ACCCACCAGT 
GTCGCTACTG TGATTCCACA ACAGCAGCAG ACTACTCAGA ACCAACCTTC CAGAATGGAC 
CGGAGTGGAA TGGGGGTAGG GGCTGGTGGT GTACCAAAAG GCGGTGCTCG TAGAGGAAAG 
GGGCCCTTGG TGAAACAGTC AAAGGTGGAA GACCTGCGTC AAGGTCAAGA CACTCTAGAA 
GCAAAGGTGT CAGTGGAGAA GAGCTCTATT CCAATCCCAA CTATCATTGT TAAAGCCCCT 
TCCACCAGCA GTAGTGGACG CAGTAGCCAA GGCAGCAGTG TGGAGGCTGA ACCACCGCCC 
CCAGGAGAAA GTGACAGTTC TAAACCAGAA CCTGTTGTTC CCCCACCTGC ACCAACCGTG 
CCACCACCAG CCCCTCCATC TATACCTGCT CCTCCACCCC CATCACTGCC ATCACTTCGT 
ACTCAAGAGA GTCTGGACTT CACCAGCCAG TTTGGTGCTG CCATTGTAGG GGCAGCTCGC 
AGGGACAGAG AACGATTTCA TGAAGCACGA CGCAAGAGTG CCTCCTTTTT TCTTTCTGCT 
GAGGAAGAGT TGAGTGGAGC TGGAGGAGCA GGAGGGCGGA CTCAGACTTC TCAACAGCAG 
CTGAGTTCCC AGCCTTCACC ACGCCTGCGA CCATCCAAAT CTATAGATGA AGGCATGTTC 
TCAGGTGATA CTTTTATCCA TCACACACGC AGTATGCCTC CTGCATTTGG CTTGCCTGAG 
TACTCCTCAC CTGCTCCTCA AGGGGATTAC CAGCCTAAGT CTGTACCTGC TGACTTCTAT 
GGGGCAGGAT CCAGACAACA GCAGCAGCAG CAGCAGCAGC AAGCCCCCAC CACAACTTTT 
ATTCACCCAC TAACTGGAAA AGTACTGGAC CCTTCATCCC CTCTAGGCTT GGCTTTGGCT 
GCACGGGAAC GTGCCTTGAA GGATGATGGA CGTATACGTA GGGAAAGGAC CACTGAACAT 
CATTTTACCC GTCAGTTGTC AAGCATTGGG GCCTTTTCCT CATCCACTTC ACAGCCAACA 
TCACACATTT CCTACCCAAT GGCCTCGTCT TCCTCTGCAA CCTCCTCTGC TGCTCAATCC 
ACAAACACCT CTTCATATCT TGTTACCTCC TCATCATTAG CTGCCACCAC CACATCCACT 
CGGCCTCCAT CTCCTAGGAT CCTGCGTGGA GCAGGGAGTA GCTGGAGTGA AGAAGCTGGG 
GTTGGAGAGC GTGCGGATAG GGAAGCAGCT GGCACCTCTG CTCCCAGAGA AGGCCTAAGG 
GTTCGTTTCT CCGAGGATAA AACTGTCCAC ACACACCACT ATCAATCTCA GCAATATCAG 
CCCACCTTTA AAGAGCGTGA AAGAGAACGT CAAAGAGAGA GGTCAAGAGA AAGAGAGAGG 
GAAAGAGAGA GGGAAAGAGA AATAGAAAAA GCTCAAACCC AGTATCAGCA ACAGTCATCA 
CAACCAATGA CCCAGCAGCC TCGACGCCCC TCATTCTTGA GGATGGAGAG TGAAGCTGGA 
GCGTACATTC TTTCATTAAC GCCTCCATCT CCTCCTCCAA CAGCAGCCCC TATGCGTCCT 
ACAGCAGGAG CCACAGGTGA AAGTGGAAGT GGTACAAGCC TGATGGTTCT GCCTCCCCCA 
GCTCCCTCAG TAGATGCAGA TGATGAGTTT GTGTTTGCTG ATCCTCTTCC TCCACCTCTA 
GAGTTTGCCA ATAGTTTTGA TCGTGGTTTG GGTTACTCTG CGATGATTTC TCACAATGTT 
GCAGCCCGTC AGCAGCGTCA TCCTCCACCA CCCCCTCCCC CACCACCCCC GCCCACTTCG 
AAAGATTCCT TTATGACAGG CTTTCCAGCT CACACACCCC TACCGTCACC TCAGGCAGGA 
GATTCCACAA CTTCCAGTCT TACCTCCTAT GACAGTGAGG TTGCAAACCT AACACAATCT 
GCCCCATCGC CCTCCCCATC TTCTCCCAAC CCACTACCAC CTCACTCCCC CTCCACCCTT 
CAGACATCTG GACCACCTCC ACCTCCCTCT GTATCACCTC CTCCACCACC CAACAGCTAC 
CATAGACCTT TTGGCTTATC TCAGTCACAA CATTTATACA ACAGTACACA CACAACTGGA 
CGCTCCTCCT CTCCCACCCC ACCTCTCTCT ATTCCAGCTC CACCTGCCTA CCGGGGGCCA 
CCAACAGCAT CATCCACCCC TGCAACTTCT GTATCAATCA GAGGTGCCTC AACTGTGACC 
GCAAGCTGTT CCACAACTGC TTCTACAACT GTCACCTCTC CTAGTACTAC TGCTGCTGCC 
ACACCACACA CACGCATAGC AACTGTCTCC ACAGTAGCTA CAGGGGGTAG TAGGCGTCCC 
AGTGCAGAGC ATCATCCACC ACCTAGTGCA ACCAAGAGTG GTGAGTCCCA GGAGACAGTG 
GTAGATTCTG GGATTGAGGA GCTGGACAGC CGAAGCAGCA GTGATCATCA CTTAGACAGC 
ATTCTTGCAC TCAGTGGTAG TGGGGTCAGA GAGAGGTTGG AACGAGCAGG GATGAGTAGC 
ATAGGTGGAA TTGGAGGGAG AGATGGTGCA ACTGAGGTTG ACAGGACAAG TGGGGCAGAC 
TTTTTAGAGT CTTACATGAG CTACCTTGAT GGGCAGACTT TTGAAATGCA CAATGCCAAA 
GCCGCTAGCA CTGCATCCAC CTACCCAAAA ACACAGAGGT ACAGAGAAGG CACCCGGGCT 
GGAGATCTTC GACGCCAGAC CAGCACAGCA CCTCCTGCAT ACCACCGTCG TAGAGAGGAG 
GAAGAGGAAG AGGATGAGGC CGAGGAGGTA GAGGAGGAGG ATTTGAGTGC CCGTGATGGC 
TATGGGATGA GGGAAGGGCC AGGGATTGCT CGCTCCTCTG TAATGTACTT TGACCGGCCC 
CGCACTCCAG AGATAAAACC ACTGTGGGGA GATGGGTCGA CTGGAGCATC ACAGACCATG 
GGGGAGGGAG GGGGCCAGAT AGTGGGAACA TATGTGGAGC CACGTAAACT ACATCCTCCC 
ATGACTGGTA TGAAAGCAAA CATCATTAAT GAACTAAGCA CCAAATTACA GCAGAGAAGT 
AAAGGAACAG AGAACTGGGG GGCCCAGAGA TCATTGAGCA GACATAGACA CAGGTTCTCA 
GAGGACTCAA CAGGTGCACT GAGTCCTCCC TCTGTCCGCT CTAGCTCCCC ATCTCCAGTT 
CAGCTCTCTC AGCCTGCCTT GTCTCCCTCT CCCACTCCTT CCTCCCAGCC ACTTTACTCA 
AACTGGGCTC GATCTCCTTC CCCTCAGCCC CCTGCTGCCT CATCCCAGCC CCCTCCCCGA 
TCCCATTCAC CTGTTTCCCC TTCATCATAT TCACCATACC CCACCTCACC CAAACACCGG 
CCTGTTTATC GCACCAAAGC TCTGGAGTTC CAATTTATTC CCAGTCGAGA GTCACGAAAG 
TCAGAGTCGC ATCATGCCCA GCGCAGACGA GCACCCAGCC CTTTAATTTC TCCATCTGAC 
CGTCCCAAGC TTGGCCCTCC CCGCCCCTCT TCCCTTCCTA TTCTCCCCAC TACACCCCTC 
TATAGCAGCC TGTATGACCT TCGTGGTTCT ATTACTCCAC CCTCACCTGC CAATCCCCTT 
GGGGACCCCT ACTTTTCCCC CTCCCCCCCT CTATTTGCCC CCTCGGGTGC TCCCCCTCCT 
CCAAATTCCT CTCTGGTGGT CTCACGGTCC CTGTCACCCA CTCACTTCCT GTCAGGGACT 
TCATCCCCAC CTCTCCACCC CATTCCTCCA CCCACTTGCC TGAGTTACCC CCATCTTCCA 
CCCCCCTCAC CCACAAAGCC CTTTGCCTCA AAACCTCTCC CATACTGGAC CAAGTATGAT 
GTTGCAGATT GGCTGGGCTA CCTGAACCTG GGTGAACACA GGGAGCGATT CTTAGACAAT 
GAAATTGATG GCACGCACCT GCCCTCTCTT ACCAAGGATG ACTACCTGGA CCTGGGTGTG 
ACCCGAGTTG GCCATCGCAT GAACATAGAG AGGGCGCTCA GGAGAGTTAT GGACAGGCTG 
TCTTCTAGCA CTTTCCCCAT TTCTGCTCTC TCTCCTCGAA ATGGACGGAC AAACAGAAGG 
AGAGATGAGG CCAACCGGAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329755.1
CDS	103..5526
Translation	MAMGGCGEEDLSLSLSLSSSWPSLGPMNRSVWFIYSDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMVDRASAPWKKKSEFESSQATEKKRTVYQMALNKLDEILAAAQQTINTNEAPGPRVQGVKRERGRGFYNEPNFDQTGMGMTSSGAGLGYDRGQFVSGHGPPHGMLRQKSIGVPEEEKQFLHPPAMKFARSLSVPGPDEIPPPPTTAPPEPPFSSAPPLGFRGKTSQQPSVSMSQSTSSAAHYQLYSQSVHVTHGPRDRSPGPPTGSGTGDLAYSHAQAHPSVPSRTASRKVIGYTGDLAGAGGVAGAKAAALRRGYSNAVPPTSVATVIPQQQQTTQNQPSRMDRSGMGVGAGGVPKGGARRGKGPLVKQSKVEDLRQGQDTLEAKVSVEKSSIPIPTIIVKAPSTSSSGRSSQGSSVEAEPPPPGESDSSKPEPVVPPPAPTVPPPAPPSIPAPPPPSLPSLRTQESLDFTSQFGAAIVGAARRDRERFHEARRKSASFFLSAEEELSGAGGAGGRTQTSQQQLSSQPSPRLRPSKSIDEGMFSGDTFIHHTRSMPPAFGLPEYSSPAPQGDYQPKSVPADFYGAGSRQQQQQQQQQAPTTTFIHPLTGKVLDPSSPLGLALAARERALKDDGRIRRERTTEHHFTRQLSSIGAFSSSTSQPTSHISYPMASSSSATSSAAQSTNTSSYLVTSSSLAATTTSTRPPSPRILRGAGSSWSEEAGVGERADREAAGTSAPREGLRVRFSEDKTVHTHHYQSQQYQPTFKERERERQRERSREREREREREREIEKAQTQYQQQSSQPMTQQPRRPSFLRMESEAGAYILSLTPPSPPPTAAPMRPTAGATGESGSGTSLMVLPPPAPSVDADDEFVFADPLPPPLEFANSFDRGLGYSAMISHNVAARQQRHPPPPPPPPPPPTSKDSFMTGFPAHTPLPSPQAGDSTTSSLTSYDSEVANLTQSAPSPSPSSPNPLPPHSPSTLQTSGPPPPPSVSPPPPPNSYHRPFGLSQSQHLYNSTHTTGRSSSPTPPLSIPAPPAYRGPPTASSTPATSVSIRGASTVTASCSTTASTTVTSPSTTAAATPHTRIATVSTVATGGSRRPSAEHHPPPSATKSGESQETVVDSGIEELDSRSSSDHHLDSILALSGSGVRERLERAGMSSIGGIGGRDGATEVDRTSGADFLESYMSYLDGQTFEMHNAKAASTASTYPKTQRYREGTRAGDLRRQTSTAPPAYHRRREEEEEEDEAEEVEEEDLSARDGYGMREGPGIARSSVMYFDRPRTPEIKPLWGDGSTGASQTMGEGGGQIVGTYVEPRKLHPPMTGMKANIINELSTKLQQRSKGTENWGAQRSLSRHRHRFSEDSTGALSPPSVRSSSPSPVQLSQPALSPSPTPSSQPLYSNWARSPSPQPPAASSQPPPRSHSPVSPSSYSPYPTSPKHRPVYRTKALEFQFIPSRESRKSESHHAQRRRAPSPLISPSDRPKLGPPRPSSLPILPTTPLYSSLYDLRGSITPPSPANPLGDPYFSPSPPLFAPSGAPPPPNSSLVVSRSLSPTHFLSGTSSPPLHPIPPPTCLSYPHLPPPSPTKPFASKPLPYWTKYDVADWLGYLNLGEHRERFLDNEIDGTHLPSLTKDDYLDLGVTRVGHRMNIERALRRVMDRLSSSTFPISALSPRNGRTNRRRDEANRS

Target ORF information:

RefSeq Version	XM_021474080.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474080.1

ORF Insert Sequence:

ATGGCGATGG GAGGATGCGG AGAGGAGGAT CTCTCTCTCT CCCTCTCCCT CTCGTCGTCC 
TGGCCATCGC TAGGCCCTAT GAATCGCAGC GTCTGGTTCA TTTACAGTGA CTACATAATC 
AAGGAGAAGA CAGTCCTCCT GCAGAAAAAA GACAATGAAG GTTTTGGCTT TGTGCTGAGA 
GGAGCCAAGG CTCAGACCCC TATAGAAGAG TTCACTCCCA CCCCAGCCTT TCCAGCACTG 
CAGTACCTTG AGTCTGTCGA CGAGGGAGGC GTGGCCTGGA GAGCTGGTCT GCGAATGGGA 
GACTTCCTTA TAGAGGTGAA TGGTCAGAAT GTGGTTAAGG TGGGACACAG GCAGGTGGTC 
AATATGATCA GGCAAGGCGG TAACAGTCTC ATGGTCAAGG TTGTCATGGT TACTCGAAAT 
CCTGATATGG AGGAAGGGGT CAGAAAGAAA ATCCCACAGC AGAGTAAAAG ACTGAGCACA 
CCGGCTATCG CTCTGCGCTC CAAATCCATG ACATCAGAGC TGGAAGAGAT GGTGGACAGA 
GCCTCTGCCC CATGGAAAAA AAAATCAGAG TTCGAATCTT CACAAGCTAC AGAGAAGAAG 
AGGACAGTTT ATCAGATGGC TTTGAATAAA CTGGATGAGA TTTTAGCAGC AGCACAGCAG 
ACCATTAATA CAAATGAGGC TCCAGGGCCC CGGGTTCAGG GGGTAAAAAG AGAACGGGGC 
AGAGGCTTTT ATAATGAGCC AAATTTCGAC CAAACTGGAA TGGGGATGAC ATCATCTGGG 
GCAGGGTTAG GCTACGACCG TGGTCAATTT GTGTCTGGCC ATGGCCCCCC GCACGGTATG 
CTGCGACAGA AGTCTATCGG GGTTCCTGAG GAGGAGAAGC AGTTTCTTCA TCCTCCAGCC 
ATGAAGTTTG CCCGTAGTCT TTCAGTACCT GGCCCAGATG AAATCCCTCC TCCACCTACT 
ACAGCACCCC CTGAGCCCCC TTTTTCCTCA GCTCCTCCGC TTGGCTTTAG GGGCAAGACA 
TCTCAACAGC CGTCTGTTTC TATGTCTCAG TCTACTTCCT CTGCTGCACA TTATCAACTC 
TACTCACAGT CAGTACATGT TACTCACGGA CCAAGGGACA GGTCCCCTGG ACCACCTACT 
GGGTCTGGAA CTGGGGACCT TGCTTATTCC CATGCCCAAG CCCACCCCTC TGTACCAAGT 
AGGACTGCCT CCAGGAAGGT GATTGGGTAC ACAGGAGATC TTGCAGGGGC TGGAGGAGTG 
GCTGGGGCCA AAGCTGCAGC ATTGAGAAGG GGTTACAGCA ATGCAGTCCC ACCCACCAGT 
GTCGCTACTG TGATTCCACA ACAGCAGCAG ACTACTCAGA ACCAACCTTC CAGAATGGAC 
CGGAGTGGAA TGGGGGTAGG GGCTGGTGGT GTACCAAAAG GCGGTGCTCG TAGAGGAAAG 
GGGCCCTTGG TGAAACAGTC AAAGGTGGAA GACCTGCGTC AAGGTCAAGA CACTCTAGAA 
GCAAAGGTGT CAGTGGAGAA GAGCTCTATT CCAATCCCAA CTATCATTGT TAAAGCCCCT 
TCCACCAGCA GTAGTGGACG CAGTAGCCAA GGCAGCAGTG TGGAGGCTGA ACCACCGCCC 
CCAGGAGAAA GTGACAGTTC TAAACCAGAA CCTGTTGTTC CCCCACCTGC ACCAACCGTG 
CCACCACCAG CCCCTCCATC TATACCTGCT CCTCCACCCC CATCACTGCC ATCACTTCGT 
ACTCAAGAGA GTCTGGACTT CACCAGCCAG TTTGGTGCTG CCATTGTAGG GGCAGCTCGC 
AGGGACAGAG AACGATTTCA TGAAGCACGA CGCAAGAGTG CCTCCTTTTT TCTTTCTGCT 
GAGGAAGAGT TGAGTGGAGC TGGAGGAGCA GGAGGGCGGA CTCAGACTTC TCAACAGCAG 
CTGAGTTCCC AGCCTTCACC ACGCCTGCGA CCATCCAAAT CTATAGATGA AGGCATGTTC 
TCAGGTGATA CTTTTATCCA TCACACACGC AGTATGCCTC CTGCATTTGG CTTGCCTGAG 
TACTCCTCAC CTGCTCCTCA AGGGGATTAC CAGCCTAAGT CTGTACCTGC TGACTTCTAT 
GGGGCAGGAT CCAGACAACA GCAGCAGCAG CAGCAGCAGC AAGCCCCCAC CACAACTTTT 
ATTCACCCAC TAACTGGAAA AGTACTGGAC CCTTCATCCC CTCTAGGCTT GGCTTTGGCT 
GCACGGGAAC GTGCCTTGAA GGATGATGGA CGTATACGTA GGGAAAGGAC CACTGAACAT 
CATTTTACCC GTCAGTTGTC AAGCATTGGG GCCTTTTCCT CATCCACTTC ACAGCCAACA 
TCACACATTT CCTACCCAAT GGCCTCGTCT TCCTCTGCAA CCTCCTCTGC TGCTCAATCC 
ACAAACACCT CTTCATATCT TGTTACCTCC TCATCATTAG CTGCCACCAC CACATCCACT 
CGGCCTCCAT CTCCTAGGAT CCTGCGTGGA GCAGGGAGTA GCTGGAGTGA AGAAGCTGGG 
GTTGGAGAGC GTGCGGATAG GGAAGCAGCT GGCACCTCTG CTCCCAGAGA AGGCCTAAGG 
GTTCGTTTCT CCGAGGATAA AACTGTCCAC ACACACCACT ATCAATCTCA GCAATATCAG 
CCCACCTTTA AAGAGCGTGA AAGAGAACGT CAAAGAGAGA GGTCAAGAGA AAGAGAGAGG 
GAAAGAGAGA GGGAAAGAGA AATAGAAAAA GCTCAAACCC AGTATCAGCA ACAGTCATCA 
CAACCAATGA CCCAGCAGCC TCGACGCCCC TCATTCTTGA GGATGGAGAG TGAAGCTGGA 
GCGTACATTC TTTCATTAAC GCCTCCATCT CCTCCTCCAA CAGCAGCCCC TATGCGTCCT 
ACAGCAGGAG CCACAGGTGA AAGTGGAAGT GGTACAAGCC TGATGGTTCT GCCTCCCCCA 
GCTCCCTCAG TAGATGCAGA TGATGAGTTT GTGTTTGCTG ATCCTCTTCC TCCACCTCTA 
GAGTTTGCCA ATAGTTTTGA TCGTGGTTTG GGTTACTCTG CGATGATTTC TCACAATGTT 
GCAGCCCGTC AGCAGCGTCA TCCTCCACCA CCCCCTCCCC CACCACCCCC GCCCACTTCG 
AAAGATTCCT TTATGACAGG CTTTCCAGCT CACACACCCC TACCGTCACC TCAGGCAGGA 
GATTCCACAA CTTCCAGTCT TACCTCCTAT GACAGTGAGG TTGCAAACCT AACACAATCT 
GCCCCATCGC CCTCCCCATC TTCTCCCAAC CCACTACCAC CTCACTCCCC CTCCACCCTT 
CAGACATCTG GACCACCTCC ACCTCCCTCT GTATCACCTC CTCCACCACC CAACAGCTAC 
CATAGACCTT TTGGCTTATC TCAGTCACAA CATTTATACA ACAGTACACA CACAACTGGA 
CGCTCCTCCT CTCCCACCCC ACCTCTCTCT ATTCCAGCTC CACCTGCCTA CCGGGGGCCA 
CCAACAGCAT CATCCACCCC TGCAACTTCT GTATCAATCA GAGGTGCCTC AACTGTGACC 
GCAAGCTGTT CCACAACTGC TTCTACAACT GTCACCTCTC CTAGTACTAC TGCTGCTGCC 
ACACCACACA CACGCATAGC AACTGTCTCC ACAGTAGCTA CAGGGGGTAG TAGGCGTCCC 
AGTGCAGAGC ATCATCCACC ACCTAGTGCA ACCAAGAGTG GTGAGTCCCA GGAGACAGTG 
GTAGATTCTG GGATTGAGGA GCTGGACAGC CGAAGCAGCA GTGATCATCA CTTAGACAGC 
ATTCTTGCAC TCAGTGGTAG TGGGGTCAGA GAGAGGTTGG AACGAGCAGG GATGAGTAGC 
ATAGGTGGAA TTGGAGGGAG AGATGGTGCA ACTGAGGTTG ACAGGACAAG TGGGGCAGAC 
TTTTTAGAGT CTTACATGAG CTACCTTGAT GGGCAGACTT TTGAAATGCA CAATGCCAAA 
GCCGCTAGCA CTGCATCCAC CTACCCAAAA ACACAGAGGT ACAGAGAAGG CACCCGGGCT 
GGAGATCTTC GACGCCAGAC CAGCACAGCA CCTCCTGCAT ACCACCGTCG TAGAGAGGAG 
GAAGAGGAAG AGGATGAGGC CGAGGAGGTA GAGGAGGAGG ATTTGAGTGC CCGTGATGGC 
TATGGGATGA GGGAAGGGCC AGGGATTGCT CGCTCCTCTG TAATGTACTT TGACCGGCCC 
CGCACTCCAG AGATAAAACC ACTGTGGGGA GATGGGTCGA CTGGAGCATC ACAGACCATG 
GGGGAGGGAG GGGGCCAGAT AGTGGGAACA TATGTGGAGC CACGTAAACT ACATCCTCCC 
ATGACTGGTA TGAAAGCAAA CATCATTAAT GAACTAAGCA CCAAATTACA GCAGAGAAGT 
AAAGGAACAG AGAACTGGGG GGCCCAGAGA TCATTGAGCA GACATAGACA CAGGTTCTCA 
GAGGACTCAA CAGGTGCACT GAGTCCTCCC TCTGTCCGCT CTAGCTCCCC ATCTCCAGTT 
CAGCTCTCTC AGCCTGCCTT GTCTCCCTCT CCCACTCCTT CCTCCCAGCC ACTTTACTCA 
AACTGGGCTC GATCTCCTTC CCCTCAGCCC CCTGCTGCCT CATCCCAGCC CCCTCCCCGA 
TCCCATTCAC CTGTTTCCCC TTCATCATAT TCACCATACC CCACCTCACC CAAACACCGG 
CCTGTTTATC GCACCAAAGC TCTGGAGTTC CAATTTATTC CCAGTCGAGA GTCACGAAAG 
TCAGAGTCGC ATCATGCCCA GCGCAGACGA GCACCCAGCC CTTTAATTTC TCCATCTGAC 
CGTCCCAAGC TTGGCCCTCC CCGCCCCTCT TCCCTTCCTA TTCTCCCCAC TACACCCCTC 
TATAGCAGCC TGTATGACCT TCGTGGTTCT ATTACTCCAC CCTCACCTGC CAATCCCCTT 
GGGGACCCCT ACTTTTCCCC CTCCCCCCCT CTATTTGCCC CCTCGGGTGC TCCCCCTCCT 
CCAAATTCCT CTCTGGTGGT CTCACGGTCC CTGTCACCCA CTCACTTCCT GTCAGGGACT 
TCATCCCCAC CTCTCCACCC CATTCCTCCA CCCACTTGCC TGAGTTACCC CCATCTTCCA 
CCCCCCTCAC CCACAAAGCC CTTTGCCTCA AAACCTCTCC CATACTGGAC CAAGTATGAT 
GTTGCAGATT GGCTGGGCTA CCTGAACCTG GGTGAACACA GGGAGCGATT CTTAGACAAT 
GAAATTGATG GCACGCACCT GCCCTCTCTT ACCAAGGATG ACTACCTGGA CCTGGGTGTG 
ACCCGAGTTG GCCATCGCAT GAACATAGAG AGGGCGCTCA GGAGAGTTAT GGACAGGCTG 
TCTTCTAGCA CTTTCCCCAT TTCTGCTCTC TCTCCTCGAA ATGGACGGAC AAACAGAAGG 
AGAGATGAGG CCAACCGGAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_021471436.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327111.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X6, mRNA.

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CloneID	ODa141768
Clone ID Related Accession (Same CDS sequence)	XM_021474080.1 , XM_021471436.1
Accession Version	XM_021471436.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394533.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATGG GAGGATGCGG AGAGGAGGAT CTCTCTCTCT CCCTCTCCCT CTCGTCGTCC 
TGGCCATCGC TAGGCCCTAT GAATCGCAGC GTCTGGTTCA TTTACAGTGA CTACATAATC 
AAGGAGAAGA CAGTCCTCCT GCAGAAAAAA GACAATGAAG GTTTTGGCTT TGTGCTGAGA 
GGAGCCAAGG CTCAGACCCC TATAGAAGAG TTCACTCCCA CCCCAGCCTT TCCAGCACTG 
CAGTACCTTG AGTCTGTCGA CGAGGGAGGC GTGGCCTGGA GAGCTGGTCT GCGAATGGGA 
GACTTCCTTA TAGAGGTGAA TGGTCAGAAT GTGGTTAAGG TGGGACACAG GCAGGTGGTC 
AATATGATCA GGCAAGGCGG TAACAGTCTC ATGGTCAAGG TTGTCATGGT TACTCGAAAT 
CCTGATATGG AGGAAGGGGT CAGAAAGAAA ATCCCACAGC AGAGTAAAAG ACTGAGCACA 
CCGGCTATCG CTCTGCGCTC CAAATCCATG ACATCAGAGC TGGAAGAGAT GGTGGACAGA 
GCCTCTGCCC CATGGAAAAA AAAATCAGAG TTCGAATCTT CACAAGCTAC AGAGAAGAAG 
AGGACAGTTT ATCAGATGGC TTTGAATAAA CTGGATGAGA TTTTAGCAGC AGCACAGCAG 
ACCATTAATA CAAATGAGGC TCCAGGGCCC CGGGTTCAGG GGGTAAAAAG AGAACGGGGC 
AGAGGCTTTT ATAATGAGCC AAATTTCGAC CAAACTGGAA TGGGGATGAC ATCATCTGGG 
GCAGGGTTAG GCTACGACCG TGGTCAATTT GTGTCTGGCC ATGGCCCCCC GCACGGTATG 
CTGCGACAGA AGTCTATCGG GGTTCCTGAG GAGGAGAAGC AGTTTCTTCA TCCTCCAGCC 
ATGAAGTTTG CCCGTAGTCT TTCAGTACCT GGCCCAGATG AAATCCCTCC TCCACCTACT 
ACAGCACCCC CTGAGCCCCC TTTTTCCTCA GCTCCTCCGC TTGGCTTTAG GGGCAAGACA 
TCTCAACAGC CGTCTGTTTC TATGTCTCAG TCTACTTCCT CTGCTGCACA TTATCAACTC 
TACTCACAGT CAGTACATGT TACTCACGGA CCAAGGGACA GGTCCCCTGG ACCACCTACT 
GGGTCTGGAA CTGGGGACCT TGCTTATTCC CATGCCCAAG CCCACCCCTC TGTACCAAGT 
AGGACTGCCT CCAGGAAGGT GATTGGGTAC ACAGGAGATC TTGCAGGGGC TGGAGGAGTG 
GCTGGGGCCA AAGCTGCAGC ATTGAGAAGG GGTTACAGCA ATGCAGTCCC ACCCACCAGT 
GTCGCTACTG TGATTCCACA ACAGCAGCAG ACTACTCAGA ACCAACCTTC CAGAATGGAC 
CGGAGTGGAA TGGGGGTAGG GGCTGGTGGT GTACCAAAAG GCGGTGCTCG TAGAGGAAAG 
GGGCCCTTGG TGAAACAGTC AAAGGTGGAA GACCTGCGTC AAGGTCAAGA CACTCTAGAA 
GCAAAGGTGT CAGTGGAGAA GAGCTCTATT CCAATCCCAA CTATCATTGT TAAAGCCCCT 
TCCACCAGCA GTAGTGGACG CAGTAGCCAA GGCAGCAGTG TGGAGGCTGA ACCACCGCCC 
CCAGGAGAAA GTGACAGTTC TAAACCAGAA CCTGTTGTTC CCCCACCTGC ACCAACCGTG 
CCACCACCAG CCCCTCCATC TATACCTGCT CCTCCACCCC CATCACTGCC ATCACTTCGT 
ACTCAAGAGA GTCTGGACTT CACCAGCCAG TTTGGTGCTG CCATTGTAGG GGCAGCTCGC 
AGGGACAGAG AACGATTTCA TGAAGCACGA CGCAAGAGTG CCTCCTTTTT TCTTTCTGCT 
GAGGAAGAGT TGAGTGGAGC TGGAGGAGCA GGAGGGCGGA CTCAGACTTC TCAACAGCAG 
CTGAGTTCCC AGCCTTCACC ACGCCTGCGA CCATCCAAAT CTATAGATGA AGGCATGTTC 
TCAGGTGATA CTTTTATCCA TCACACACGC AGTATGCCTC CTGCATTTGG CTTGCCTGAG 
TACTCCTCAC CTGCTCCTCA AGGGGATTAC CAGCCTAAGT CTGTACCTGC TGACTTCTAT 
GGGGCAGGAT CCAGACAACA GCAGCAGCAG CAGCAGCAGC AAGCCCCCAC CACAACTTTT 
ATTCACCCAC TAACTGGAAA AGTACTGGAC CCTTCATCCC CTCTAGGCTT GGCTTTGGCT 
GCACGGGAAC GTGCCTTGAA GGATGATGGA CGTATACGTA GGGAAAGGAC CACTGAACAT 
CATTTTACCC GTCAGTTGTC AAGCATTGGG GCCTTTTCCT CATCCACTTC ACAGCCAACA 
TCACACATTT CCTACCCAAT GGCCTCGTCT TCCTCTGCAA CCTCCTCTGC TGCTCAATCC 
ACAAACACCT CTTCATATCT TGTTACCTCC TCATCATTAG CTGCCACCAC CACATCCACT 
CGGCCTCCAT CTCCTAGGAT CCTGCGTGGA GCAGGGAGTA GCTGGAGTGA AGAAGCTGGG 
GTTGGAGAGC GTGCGGATAG GGAAGCAGCT GGCACCTCTG CTCCCAGAGA AGGCCTAAGG 
GTTCGTTTCT CCGAGGATAA AACTGTCCAC ACACACCACT ATCAATCTCA GCAATATCAG 
CCCACCTTTA AAGAGCGTGA AAGAGAACGT CAAAGAGAGA GGTCAAGAGA AAGAGAGAGG 
GAAAGAGAGA GGGAAAGAGA AATAGAAAAA GCTCAAACCC AGTATCAGCA ACAGTCATCA 
CAACCAATGA CCCAGCAGCC TCGACGCCCC TCATTCTTGA GGATGGAGAG TGAAGCTGGA 
GCGTACATTC TTTCATTAAC GCCTCCATCT CCTCCTCCAA CAGCAGCCCC TATGCGTCCT 
ACAGCAGGAG CCACAGGTGA AAGTGGAAGT GGTACAAGCC TGATGGTTCT GCCTCCCCCA 
GCTCCCTCAG TAGATGCAGA TGATGAGTTT GTGTTTGCTG ATCCTCTTCC TCCACCTCTA 
GAGTTTGCCA ATAGTTTTGA TCGTGGTTTG GGTTACTCTG CGATGATTTC TCACAATGTT 
GCAGCCCGTC AGCAGCGTCA TCCTCCACCA CCCCCTCCCC CACCACCCCC GCCCACTTCG 
AAAGATTCCT TTATGACAGG CTTTCCAGCT CACACACCCC TACCGTCACC TCAGGCAGGA 
GATTCCACAA CTTCCAGTCT TACCTCCTAT GACAGTGAGG TTGCAAACCT AACACAATCT 
GCCCCATCGC CCTCCCCATC TTCTCCCAAC CCACTACCAC CTCACTCCCC CTCCACCCTT 
CAGACATCTG GACCACCTCC ACCTCCCTCT GTATCACCTC CTCCACCACC CAACAGCTAC 
CATAGACCTT TTGGCTTATC TCAGTCACAA CATTTATACA ACAGTACACA CACAACTGGA 
CGCTCCTCCT CTCCCACCCC ACCTCTCTCT ATTCCAGCTC CACCTGCCTA CCGGGGGCCA 
CCAACAGCAT CATCCACCCC TGCAACTTCT GTATCAATCA GAGGTGCCTC AACTGTGACC 
GCAAGCTGTT CCACAACTGC TTCTACAACT GTCACCTCTC CTAGTACTAC TGCTGCTGCC 
ACACCACACA CACGCATAGC AACTGTCTCC ACAGTAGCTA CAGGGGGTAG TAGGCGTCCC 
AGTGCAGAGC ATCATCCACC ACCTAGTGCA ACCAAGAGTG GTGAGTCCCA GGAGACAGTG 
GTAGATTCTG GGATTGAGGA GCTGGACAGC CGAAGCAGCA GTGATCATCA CTTAGACAGC 
ATTCTTGCAC TCAGTGGTAG TGGGGTCAGA GAGAGGTTGG AACGAGCAGG GATGAGTAGC 
ATAGGTGGAA TTGGAGGGAG AGATGGTGCA ACTGAGGTTG ACAGGACAAG TGGGGCAGAC 
TTTTTAGAGT CTTACATGAG CTACCTTGAT GGGCAGACTT TTGAAATGCA CAATGCCAAA 
GCCGCTAGCA CTGCATCCAC CTACCCAAAA ACACAGAGGT ACAGAGAAGG CACCCGGGCT 
GGAGATCTTC GACGCCAGAC CAGCACAGCA CCTCCTGCAT ACCACCGTCG TAGAGAGGAG 
GAAGAGGAAG AGGATGAGGC CGAGGAGGTA GAGGAGGAGG ATTTGAGTGC CCGTGATGGC 
TATGGGATGA GGGAAGGGCC AGGGATTGCT CGCTCCTCTG TAATGTACTT TGACCGGCCC 
CGCACTCCAG AGATAAAACC ACTGTGGGGA GATGGGTCGA CTGGAGCATC ACAGACCATG 
GGGGAGGGAG GGGGCCAGAT AGTGGGAACA TATGTGGAGC CACGTAAACT ACATCCTCCC 
ATGACTGGTA TGAAAGCAAA CATCATTAAT GAACTAAGCA CCAAATTACA GCAGAGAAGT 
AAAGGAACAG AGAACTGGGG GGCCCAGAGA TCATTGAGCA GACATAGACA CAGGTTCTCA 
GAGGACTCAA CAGGTGCACT GAGTCCTCCC TCTGTCCGCT CTAGCTCCCC ATCTCCAGTT 
CAGCTCTCTC AGCCTGCCTT GTCTCCCTCT CCCACTCCTT CCTCCCAGCC ACTTTACTCA 
AACTGGGCTC GATCTCCTTC CCCTCAGCCC CCTGCTGCCT CATCCCAGCC CCCTCCCCGA 
TCCCATTCAC CTGTTTCCCC TTCATCATAT TCACCATACC CCACCTCACC CAAACACCGG 
CCTGTTTATC GCACCAAAGC TCTGGAGTTC CAATTTATTC CCAGTCGAGA GTCACGAAAG 
TCAGAGTCGC ATCATGCCCA GCGCAGACGA GCACCCAGCC CTTTAATTTC TCCATCTGAC 
CGTCCCAAGC TTGGCCCTCC CCGCCCCTCT TCCCTTCCTA TTCTCCCCAC TACACCCCTC 
TATAGCAGCC TGTATGACCT TCGTGGTTCT ATTACTCCAC CCTCACCTGC CAATCCCCTT 
GGGGACCCCT ACTTTTCCCC CTCCCCCCCT CTATTTGCCC CCTCGGGTGC TCCCCCTCCT 
CCAAATTCCT CTCTGGTGGT CTCACGGTCC CTGTCACCCA CTCACTTCCT GTCAGGGACT 
TCATCCCCAC CTCTCCACCC CATTCCTCCA CCCACTTGCC TGAGTTACCC CCATCTTCCA 
CCCCCCTCAC CCACAAAGCC CTTTGCCTCA AAACCTCTCC CATACTGGAC CAAGTATGAT 
GTTGCAGATT GGCTGGGCTA CCTGAACCTG GGTGAACACA GGGAGCGATT CTTAGACAAT 
GAAATTGATG GCACGCACCT GCCCTCTCTT ACCAAGGATG ACTACCTGGA CCTGGGTGTG 
ACCCGAGTTG GCCATCGCAT GAACATAGAG AGGGCGCTCA GGAGAGTTAT GGACAGGCTG 
TCTTCTAGCA CTTTCCCCAT TTCTGCTCTC TCTCCTCGAA ATGGACGGAC AAACAGAAGG 
AGAGATGAGG CCAACCGGAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327111.1
CDS	103..5526
Translation	MAMGGCGEEDLSLSLSLSSSWPSLGPMNRSVWFIYSDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMVDRASAPWKKKSEFESSQATEKKRTVYQMALNKLDEILAAAQQTINTNEAPGPRVQGVKRERGRGFYNEPNFDQTGMGMTSSGAGLGYDRGQFVSGHGPPHGMLRQKSIGVPEEEKQFLHPPAMKFARSLSVPGPDEIPPPPTTAPPEPPFSSAPPLGFRGKTSQQPSVSMSQSTSSAAHYQLYSQSVHVTHGPRDRSPGPPTGSGTGDLAYSHAQAHPSVPSRTASRKVIGYTGDLAGAGGVAGAKAAALRRGYSNAVPPTSVATVIPQQQQTTQNQPSRMDRSGMGVGAGGVPKGGARRGKGPLVKQSKVEDLRQGQDTLEAKVSVEKSSIPIPTIIVKAPSTSSSGRSSQGSSVEAEPPPPGESDSSKPEPVVPPPAPTVPPPAPPSIPAPPPPSLPSLRTQESLDFTSQFGAAIVGAARRDRERFHEARRKSASFFLSAEEELSGAGGAGGRTQTSQQQLSSQPSPRLRPSKSIDEGMFSGDTFIHHTRSMPPAFGLPEYSSPAPQGDYQPKSVPADFYGAGSRQQQQQQQQQAPTTTFIHPLTGKVLDPSSPLGLALAARERALKDDGRIRRERTTEHHFTRQLSSIGAFSSSTSQPTSHISYPMASSSSATSSAAQSTNTSSYLVTSSSLAATTTSTRPPSPRILRGAGSSWSEEAGVGERADREAAGTSAPREGLRVRFSEDKTVHTHHYQSQQYQPTFKERERERQRERSREREREREREREIEKAQTQYQQQSSQPMTQQPRRPSFLRMESEAGAYILSLTPPSPPPTAAPMRPTAGATGESGSGTSLMVLPPPAPSVDADDEFVFADPLPPPLEFANSFDRGLGYSAMISHNVAARQQRHPPPPPPPPPPPTSKDSFMTGFPAHTPLPSPQAGDSTTSSLTSYDSEVANLTQSAPSPSPSSPNPLPPHSPSTLQTSGPPPPPSVSPPPPPNSYHRPFGLSQSQHLYNSTHTTGRSSSPTPPLSIPAPPAYRGPPTASSTPATSVSIRGASTVTASCSTTASTTVTSPSTTAAATPHTRIATVSTVATGGSRRPSAEHHPPPSATKSGESQETVVDSGIEELDSRSSSDHHLDSILALSGSGVRERLERAGMSSIGGIGGRDGATEVDRTSGADFLESYMSYLDGQTFEMHNAKAASTASTYPKTQRYREGTRAGDLRRQTSTAPPAYHRRREEEEEEDEAEEVEEEDLSARDGYGMREGPGIARSSVMYFDRPRTPEIKPLWGDGSTGASQTMGEGGGQIVGTYVEPRKLHPPMTGMKANIINELSTKLQQRSKGTENWGAQRSLSRHRHRFSEDSTGALSPPSVRSSSPSPVQLSQPALSPSPTPSSQPLYSNWARSPSPQPPAASSQPPPRSHSPVSPSSYSPYPTSPKHRPVYRTKALEFQFIPSRESRKSESHHAQRRRAPSPLISPSDRPKLGPPRPSSLPILPTTPLYSSLYDLRGSITPPSPANPLGDPYFSPSPPLFAPSGAPPPPNSSLVVSRSLSPTHFLSGTSSPPLHPIPPPTCLSYPHLPPPSPTKPFASKPLPYWTKYDVADWLGYLNLGEHRERFLDNEIDGTHLPSLTKDDYLDLGVTRVGHRMNIERALRRVMDRLSSSTFPISALSPRNGRTNRRRDEANRS

Target ORF information:

RefSeq Version	XM_021471436.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471436.1

ORF Insert Sequence:

ATGGCGATGG GAGGATGCGG AGAGGAGGAT CTCTCTCTCT CCCTCTCCCT CTCGTCGTCC 
TGGCCATCGC TAGGCCCTAT GAATCGCAGC GTCTGGTTCA TTTACAGTGA CTACATAATC 
AAGGAGAAGA CAGTCCTCCT GCAGAAAAAA GACAATGAAG GTTTTGGCTT TGTGCTGAGA 
GGAGCCAAGG CTCAGACCCC TATAGAAGAG TTCACTCCCA CCCCAGCCTT TCCAGCACTG 
CAGTACCTTG AGTCTGTCGA CGAGGGAGGC GTGGCCTGGA GAGCTGGTCT GCGAATGGGA 
GACTTCCTTA TAGAGGTGAA TGGTCAGAAT GTGGTTAAGG TGGGACACAG GCAGGTGGTC 
AATATGATCA GGCAAGGCGG TAACAGTCTC ATGGTCAAGG TTGTCATGGT TACTCGAAAT 
CCTGATATGG AGGAAGGGGT CAGAAAGAAA ATCCCACAGC AGAGTAAAAG ACTGAGCACA 
CCGGCTATCG CTCTGCGCTC CAAATCCATG ACATCAGAGC TGGAAGAGAT GGTGGACAGA 
GCCTCTGCCC CATGGAAAAA AAAATCAGAG TTCGAATCTT CACAAGCTAC AGAGAAGAAG 
AGGACAGTTT ATCAGATGGC TTTGAATAAA CTGGATGAGA TTTTAGCAGC AGCACAGCAG 
ACCATTAATA CAAATGAGGC TCCAGGGCCC CGGGTTCAGG GGGTAAAAAG AGAACGGGGC 
AGAGGCTTTT ATAATGAGCC AAATTTCGAC CAAACTGGAA TGGGGATGAC ATCATCTGGG 
GCAGGGTTAG GCTACGACCG TGGTCAATTT GTGTCTGGCC ATGGCCCCCC GCACGGTATG 
CTGCGACAGA AGTCTATCGG GGTTCCTGAG GAGGAGAAGC AGTTTCTTCA TCCTCCAGCC 
ATGAAGTTTG CCCGTAGTCT TTCAGTACCT GGCCCAGATG AAATCCCTCC TCCACCTACT 
ACAGCACCCC CTGAGCCCCC TTTTTCCTCA GCTCCTCCGC TTGGCTTTAG GGGCAAGACA 
TCTCAACAGC CGTCTGTTTC TATGTCTCAG TCTACTTCCT CTGCTGCACA TTATCAACTC 
TACTCACAGT CAGTACATGT TACTCACGGA CCAAGGGACA GGTCCCCTGG ACCACCTACT 
GGGTCTGGAA CTGGGGACCT TGCTTATTCC CATGCCCAAG CCCACCCCTC TGTACCAAGT 
AGGACTGCCT CCAGGAAGGT GATTGGGTAC ACAGGAGATC TTGCAGGGGC TGGAGGAGTG 
GCTGGGGCCA AAGCTGCAGC ATTGAGAAGG GGTTACAGCA ATGCAGTCCC ACCCACCAGT 
GTCGCTACTG TGATTCCACA ACAGCAGCAG ACTACTCAGA ACCAACCTTC CAGAATGGAC 
CGGAGTGGAA TGGGGGTAGG GGCTGGTGGT GTACCAAAAG GCGGTGCTCG TAGAGGAAAG 
GGGCCCTTGG TGAAACAGTC AAAGGTGGAA GACCTGCGTC AAGGTCAAGA CACTCTAGAA 
GCAAAGGTGT CAGTGGAGAA GAGCTCTATT CCAATCCCAA CTATCATTGT TAAAGCCCCT 
TCCACCAGCA GTAGTGGACG CAGTAGCCAA GGCAGCAGTG TGGAGGCTGA ACCACCGCCC 
CCAGGAGAAA GTGACAGTTC TAAACCAGAA CCTGTTGTTC CCCCACCTGC ACCAACCGTG 
CCACCACCAG CCCCTCCATC TATACCTGCT CCTCCACCCC CATCACTGCC ATCACTTCGT 
ACTCAAGAGA GTCTGGACTT CACCAGCCAG TTTGGTGCTG CCATTGTAGG GGCAGCTCGC 
AGGGACAGAG AACGATTTCA TGAAGCACGA CGCAAGAGTG CCTCCTTTTT TCTTTCTGCT 
GAGGAAGAGT TGAGTGGAGC TGGAGGAGCA GGAGGGCGGA CTCAGACTTC TCAACAGCAG 
CTGAGTTCCC AGCCTTCACC ACGCCTGCGA CCATCCAAAT CTATAGATGA AGGCATGTTC 
TCAGGTGATA CTTTTATCCA TCACACACGC AGTATGCCTC CTGCATTTGG CTTGCCTGAG 
TACTCCTCAC CTGCTCCTCA AGGGGATTAC CAGCCTAAGT CTGTACCTGC TGACTTCTAT 
GGGGCAGGAT CCAGACAACA GCAGCAGCAG CAGCAGCAGC AAGCCCCCAC CACAACTTTT 
ATTCACCCAC TAACTGGAAA AGTACTGGAC CCTTCATCCC CTCTAGGCTT GGCTTTGGCT 
GCACGGGAAC GTGCCTTGAA GGATGATGGA CGTATACGTA GGGAAAGGAC CACTGAACAT 
CATTTTACCC GTCAGTTGTC AAGCATTGGG GCCTTTTCCT CATCCACTTC ACAGCCAACA 
TCACACATTT CCTACCCAAT GGCCTCGTCT TCCTCTGCAA CCTCCTCTGC TGCTCAATCC 
ACAAACACCT CTTCATATCT TGTTACCTCC TCATCATTAG CTGCCACCAC CACATCCACT 
CGGCCTCCAT CTCCTAGGAT CCTGCGTGGA GCAGGGAGTA GCTGGAGTGA AGAAGCTGGG 
GTTGGAGAGC GTGCGGATAG GGAAGCAGCT GGCACCTCTG CTCCCAGAGA AGGCCTAAGG 
GTTCGTTTCT CCGAGGATAA AACTGTCCAC ACACACCACT ATCAATCTCA GCAATATCAG 
CCCACCTTTA AAGAGCGTGA AAGAGAACGT CAAAGAGAGA GGTCAAGAGA AAGAGAGAGG 
GAAAGAGAGA GGGAAAGAGA AATAGAAAAA GCTCAAACCC AGTATCAGCA ACAGTCATCA 
CAACCAATGA CCCAGCAGCC TCGACGCCCC TCATTCTTGA GGATGGAGAG TGAAGCTGGA 
GCGTACATTC TTTCATTAAC GCCTCCATCT CCTCCTCCAA CAGCAGCCCC TATGCGTCCT 
ACAGCAGGAG CCACAGGTGA AAGTGGAAGT GGTACAAGCC TGATGGTTCT GCCTCCCCCA 
GCTCCCTCAG TAGATGCAGA TGATGAGTTT GTGTTTGCTG ATCCTCTTCC TCCACCTCTA 
GAGTTTGCCA ATAGTTTTGA TCGTGGTTTG GGTTACTCTG CGATGATTTC TCACAATGTT 
GCAGCCCGTC AGCAGCGTCA TCCTCCACCA CCCCCTCCCC CACCACCCCC GCCCACTTCG 
AAAGATTCCT TTATGACAGG CTTTCCAGCT CACACACCCC TACCGTCACC TCAGGCAGGA 
GATTCCACAA CTTCCAGTCT TACCTCCTAT GACAGTGAGG TTGCAAACCT AACACAATCT 
GCCCCATCGC CCTCCCCATC TTCTCCCAAC CCACTACCAC CTCACTCCCC CTCCACCCTT 
CAGACATCTG GACCACCTCC ACCTCCCTCT GTATCACCTC CTCCACCACC CAACAGCTAC 
CATAGACCTT TTGGCTTATC TCAGTCACAA CATTTATACA ACAGTACACA CACAACTGGA 
CGCTCCTCCT CTCCCACCCC ACCTCTCTCT ATTCCAGCTC CACCTGCCTA CCGGGGGCCA 
CCAACAGCAT CATCCACCCC TGCAACTTCT GTATCAATCA GAGGTGCCTC AACTGTGACC 
GCAAGCTGTT CCACAACTGC TTCTACAACT GTCACCTCTC CTAGTACTAC TGCTGCTGCC 
ACACCACACA CACGCATAGC AACTGTCTCC ACAGTAGCTA CAGGGGGTAG TAGGCGTCCC 
AGTGCAGAGC ATCATCCACC ACCTAGTGCA ACCAAGAGTG GTGAGTCCCA GGAGACAGTG 
GTAGATTCTG GGATTGAGGA GCTGGACAGC CGAAGCAGCA GTGATCATCA CTTAGACAGC 
ATTCTTGCAC TCAGTGGTAG TGGGGTCAGA GAGAGGTTGG AACGAGCAGG GATGAGTAGC 
ATAGGTGGAA TTGGAGGGAG AGATGGTGCA ACTGAGGTTG ACAGGACAAG TGGGGCAGAC 
TTTTTAGAGT CTTACATGAG CTACCTTGAT GGGCAGACTT TTGAAATGCA CAATGCCAAA 
GCCGCTAGCA CTGCATCCAC CTACCCAAAA ACACAGAGGT ACAGAGAAGG CACCCGGGCT 
GGAGATCTTC GACGCCAGAC CAGCACAGCA CCTCCTGCAT ACCACCGTCG TAGAGAGGAG 
GAAGAGGAAG AGGATGAGGC CGAGGAGGTA GAGGAGGAGG ATTTGAGTGC CCGTGATGGC 
TATGGGATGA GGGAAGGGCC AGGGATTGCT CGCTCCTCTG TAATGTACTT TGACCGGCCC 
CGCACTCCAG AGATAAAACC ACTGTGGGGA GATGGGTCGA CTGGAGCATC ACAGACCATG 
GGGGAGGGAG GGGGCCAGAT AGTGGGAACA TATGTGGAGC CACGTAAACT ACATCCTCCC 
ATGACTGGTA TGAAAGCAAA CATCATTAAT GAACTAAGCA CCAAATTACA GCAGAGAAGT 
AAAGGAACAG AGAACTGGGG GGCCCAGAGA TCATTGAGCA GACATAGACA CAGGTTCTCA 
GAGGACTCAA CAGGTGCACT GAGTCCTCCC TCTGTCCGCT CTAGCTCCCC ATCTCCAGTT 
CAGCTCTCTC AGCCTGCCTT GTCTCCCTCT CCCACTCCTT CCTCCCAGCC ACTTTACTCA 
AACTGGGCTC GATCTCCTTC CCCTCAGCCC CCTGCTGCCT CATCCCAGCC CCCTCCCCGA 
TCCCATTCAC CTGTTTCCCC TTCATCATAT TCACCATACC CCACCTCACC CAAACACCGG 
CCTGTTTATC GCACCAAAGC TCTGGAGTTC CAATTTATTC CCAGTCGAGA GTCACGAAAG 
TCAGAGTCGC ATCATGCCCA GCGCAGACGA GCACCCAGCC CTTTAATTTC TCCATCTGAC 
CGTCCCAAGC TTGGCCCTCC CCGCCCCTCT TCCCTTCCTA TTCTCCCCAC TACACCCCTC 
TATAGCAGCC TGTATGACCT TCGTGGTTCT ATTACTCCAC CCTCACCTGC CAATCCCCTT 
GGGGACCCCT ACTTTTCCCC CTCCCCCCCT CTATTTGCCC CCTCGGGTGC TCCCCCTCCT 
CCAAATTCCT CTCTGGTGGT CTCACGGTCC CTGTCACCCA CTCACTTCCT GTCAGGGACT 
TCATCCCCAC CTCTCCACCC CATTCCTCCA CCCACTTGCC TGAGTTACCC CCATCTTCCA 
CCCCCCTCAC CCACAAAGCC CTTTGCCTCA AAACCTCTCC CATACTGGAC CAAGTATGAT 
GTTGCAGATT GGCTGGGCTA CCTGAACCTG GGTGAACACA GGGAGCGATT CTTAGACAAT 
GAAATTGATG GCACGCACCT GCCCTCTCTT ACCAAGGATG ACTACCTGGA CCTGGGTGTG 
ACCCGAGTTG GCCATCGCAT GAACATAGAG AGGGCGCTCA GGAGAGTTAT GGACAGGCTG 
TCTTCTAGCA CTTTCCCCAT TTCTGCTCTC TCTCCTCGAA ATGGACGGAC AAACAGAAGG 
AGAGATGAGG CCAACCGGAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_021474081.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329756.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa141769
Clone ID Related Accession (Same CDS sequence)	XM_021474081.1 , XM_021471437.1
Accession Version	XM_021474081.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGACT ACATAATCAA GGAGAAGACA GTCCTCCTGC AGAAAAAAGA CAATGAAGGT 
TTTGGCTTTG TGCTGAGAGG AGCCAAGGCT CAGACCCCTA TAGAAGAGTT CACTCCCACC 
CCAGCCTTTC CAGCACTGCA GTACCTTGAG TCTGTCGACG AGGGAGGCGT GGCCTGGAGA 
GCTGGTCTGC GAATGGGAGA CTTCCTTATA GAGGTGAATG GTCAGAATGT GGTTAAGGTG 
GGACACAGGC AGGTGGTCAA TATGATCAGG CAAGGCGGTA ACAGTCTCAT GGTCAAGGTT 
GTCATGGTTA CTCGAAATCC TGATATGGAG GAAGGGGTCA GAAAGAAAAT CCCACAGCAG 
AGTAAAAGAC TGAGCACACC GGCTATCGCT CTGCGCTCCA AATCCATGAC ATCAGAGCTG 
GAAGAGATGG TGGACAGAGC CTCTGCCCCA TGGAAAAAAA AATCAGAGTT CGAATCTTCA 
CAAGCTACAG AGAAGAAGAG GACAGTTTAT CAGATGGCTT TGAATAAACT GGATGAGATT 
TTAGCAGCAG CACAGCAGAC CATTAATACA AATGAGGCTC CAGGGCCCCG GGTTCAGGGG 
GTAAAAAGAG AACGGGGCAG AGGCTTTTAT AATGAGCCAA ATTTCGACCA AACTGGAATG 
GGGATGACAT CATCTGGGGC AGGGTTAGGC TACGACCGTG GTCAATTTGT GTCTGGCCAT 
GGCCCCCCGC ACGGTATGCT GCGACAGAAG TCTATCGGGG TTCCTGAGGA GGAGAAGCAG 
TTTCTTCATC CTCCAGCCAT GAAGTTTGCC CGTAGTCTTT CAGTACCTGG CCCAGATGAA 
ATCCCTCCTC CACCTACTAC AGCACCCCCT GAGCCCCCTT TTTCCTCAGC TCCTCCGCTT 
GGCTTTAGGG GCAAGACATC TCAACAGCCG TCTGTTTCTA TGTCTCAGTC TACTTCCTCT 
GCTGCACATT ATCAACTCTA CTCACAGTCA GTACATGTTA CTCACGGACC AAGGGACAGG 
TCCCCTGGAC CACCTACTGG GTCTGGAACT GGGGACCTTG CTTATTCCCA TGCCCAAGCC 
CACCCCTCTG TACCAAGTAG GACTGCCTCC AGGAAGGTGA TTGGGTACAC AGGAGATCTT 
GCAGGGGCTG GAGGAGTGGC TGGGGCCAAA GCTGCAGCAT TGAGAAGGGG TTACAGCAAT 
GCAGTCCCAC CCACCAGTGT CGCTACTGTG ATTCCACAAC AGCAGCAGAC TACTCAGAAC 
CAACCTTCCA GAATGGACCG GAGTGGAATG GGGGTAGGGG CTGGTGGTGT ACCAAAAGGC 
GGTGCTCGTA GAGGAAAGGG GCCCTTGGTG AAACAGTCAA AGGTGGAAGA CCTGCGTCAA 
GGTCAAGACA CTCTAGAAGC AAAGGTGTCA GTGGAGAAGA GCTCTATTCC AATCCCAACT 
ATCATTGTTA AAGCCCCTTC CACCAGCAGT AGTGGACGCA GTAGCCAAGG CAGCAGTGTG 
GAGGCTGAAC CACCGCCCCC AGGAGAAAGT GACAGTTCTA AACCAGAACC TGTTGTTCCC 
CCACCTGCAC CAACCGTGCC ACCACCAGCC CCTCCATCTA TACCTGCTCC TCCACCCCCA 
TCACTGCCAT CACTTCGTAC TCAAGAGAGT CTGGACTTCA CCAGCCAGTT TGGTGCTGCC 
ATTGTAGGGG CAGCTCGCAG GGACAGAGAA CGATTTCATG AAGCACGACG CAAGAGTGCC 
TCCTTTTTTC TTTCTGCTGA GGAAGAGTTG AGTGGAGCTG GAGGAGCAGG AGGGCGGACT 
CAGACTTCTC AACAGCAGCT GAGTTCCCAG CCTTCACCAC GCCTGCGACC ATCCAAATCT 
ATAGATGAAG GCATGTTCTC AGGTGATACT TTTATCCATC ACACACGCAG TATGCCTCCT 
GCATTTGGCT TGCCTGAGTA CTCCTCACCT GCTCCTCAAG GGGATTACCA GCCTAAGTCT 
GTACCTGCTG ACTTCTATGG GGCAGGATCC AGACAACAGC AGCAGCAGCA GCAGCAGCAA 
GCCCCCACCA CAACTTTTAT TCACCCACTA ACTGGAAAAG TACTGGACCC TTCATCCCCT 
CTAGGCTTGG CTTTGGCTGC ACGGGAACGT GCCTTGAAGG ATGATGGACG TATACGTAGG 
GAAAGGACCA CTGAACATCA TTTTACCCGT CAGTTGTCAA GCATTGGGGC CTTTTCCTCA 
TCCACTTCAC AGCCAACATC ACACATTTCC TACCCAATGG CCTCGTCTTC CTCTGCAACC 
TCCTCTGCTG CTCAATCCAC AAACACCTCT TCATATCTTG TTACCTCCTC ATCATTAGCT 
GCCACCACCA CATCCACTCG GCCTCCATCT CCTAGGATCC TGCGTGGAGC AGGGAGTAGC 
TGGAGTGAAG AAGCTGGGGT TGGAGAGCGT GCGGATAGGG AAGCAGCTGG CACCTCTGCT 
CCCAGAGAAG GCCTAAGGGT TCGTTTCTCC GAGGATAAAA CTGTCCACAC ACACCACTAT 
CAATCTCAGC AATATCAGCC CACCTTTAAA GAGCGTGAAA GAGAACGTCA AAGAGAGAGG 
TCAAGAGAAA GAGAGAGGGA AAGAGAGAGG GAAAGAGAAA TAGAAAAAGC TCAAACCCAG 
TATCAGCAAC AGTCATCACA ACCAATGACC CAGCAGCCTC GACGCCCCTC ATTCTTGAGG 
ATGGAGAGTG AAGCTGGAGC GTACATTCTT TCATTAACGC CTCCATCTCC TCCTCCAACA 
GCAGCCCCTA TGCGTCCTAC AGCAGGAGCC ACAGGTGAAA GTGGAAGTGG TACAAGCCTG 
ATGGTTCTGC CTCCCCCAGC TCCCTCAGTA GATGCAGATG ATGAGTTTGT GTTTGCTGAT 
CCTCTTCCTC CACCTCTAGA GTTTGCCAAT AGTTTTGATC GTGGTTTGGG TTACTCTGCG 
ATGATTTCTC ACAATGTTGC AGCCCGTCAG CAGCGTCATC CTCCACCACC CCCTCCCCCA 
CCACCCCCGC CCACTTCGAA AGATTCCTTT ATGACAGGCT TTCCAGCTCA CACACCCCTA 
CCGTCACCTC AGGCAGGAGA TTCCACAACT TCCAGTCTTA CCTCCTATGA CAGTGAGGTT 
GCAAACCTAA CACAATCTGC CCCATCGCCC TCCCCATCTT CTCCCAACCC ACTACCACCT 
CACTCCCCCT CCACCCTTCA GACATCTGGA CCACCTCCAC CTCCCTCTGT ATCACCTCCT 
CCACCACCCA ACAGCTACCA TAGACCTTTT GGCTTATCTC AGTCACAACA TTTATACAAC 
AGTACACACA CAACTGGACG CTCCTCCTCT CCCACCCCAC CTCTCTCTAT TCCAGCTCCA 
CCTGCCTACC GGGGGCCACC AACAGCATCA TCCACCCCTG CAACTTCTGT ATCAATCAGA 
GGTGCCTCAA CTGTGACCGC AAGCTGTTCC ACAACTGCTT CTACAACTGT CACCTCTCCT 
AGTACTACTG CTGCTGCCAC ACCACACACA CGCATAGCAA CTGTCTCCAC AGTAGCTACA 
GGGGGTAGTA GGCGTCCCAG TGCAGAGCAT CATCCACCAC CTAGTGCAAC CAAGAGTGGT 
GAGTCCCAGG AGACAGTGGT AGATTCTGGG ATTGAGGAGC TGGACAGCCG AAGCAGCAGT 
GATCATCACT TAGACAGCAT TCTTGCACTC AGTGGTAGTG GGGTCAGAGA GAGGTTGGAA 
CGAGCAGGGA TGAGTAGCAT AGGTGGAATT GGAGGGAGAG ATGGTGCAAC TGAGGTTGAC 
AGGACAAGTG GGGCAGACTT TTTAGAGTCT TACATGAGCT ACCTTGATGG GCAGACTTTT 
GAAATGCACA ATGCCAAAGC CGCTAGCACT GCATCCACCT ACCCAAAAAC ACAGAGGTAC 
AGAGAAGGCA CCCGGGCTGG AGATCTTCGA CGCCAGACCA GCACAGCACC TCCTGCATAC 
CACCGTCGTA GAGAGGAGGA AGAGGAAGAG GATGAGGCCG AGGAGGTAGA GGAGGAGGAT 
TTGAGTGCCC GTGATGGCTA TGGGATGAGG GAAGGGCCAG GGATTGCTCG CTCCTCTGTA 
ATGTACTTTG ACCGGCCCCG CACTCCAGAG ATAAAACCAC TGTGGGGAGA TGGGTCGACT 
GGAGCATCAC AGACCATGGG GGAGGGAGGG GGCCAGATAG TGGGAACATA TGTGGAGCCA 
CGTAAACTAC ATCCTCCCAT GACTGGTATG AAAGCAAACA TCATTAATGA ACTAAGCACC 
AAATTACAGC AGAGAAGTAA AGGAACAGAG AACTGGGGGG CCCAGAGATC ATTGAGCAGA 
CATAGACACA GGTTCTCAGA GGACTCAACA GGTGCACTGA GTCCTCCCTC TGTCCGCTCT 
AGCTCCCCAT CTCCAGTTCA GCTCTCTCAG CCTGCCTTGT CTCCCTCTCC CACTCCTTCC 
TCCCAGCCAC TTTACTCAAA CTGGGCTCGA TCTCCTTCCC CTCAGCCCCC TGCTGCCTCA 
TCCCAGCCCC CTCCCCGATC CCATTCACCT GTTTCCCCTT CATCATATTC ACCATACCCC 
ACCTCACCCA AACACCGGCC TGTTTATCGC ACCAAAGCTC TGGAGTTCCA ATTTATTCCC 
AGTCGAGAGT CACGAAAGTC AGAGTCGCAT CATGCCCAGC GCAGACGAGC ACCCAGCCCT 
TTAATTTCTC CATCTGACCG TCCCAAGCTT GGCCCTCCCC GCCCCTCTTC CCTTCCTATT 
CTCCCCACTA CACCCCTCTA TAGCAGCCTG TATGACCTTC GTGGTTCTAT TACTCCACCC 
TCACCTGCCA ATCCCCTTGG GGACCCCTAC TTTTCCCCCT CCCCCCCTCT ATTTGCCCCC 
TCGGGTGCTC CCCCTCCTCC AAATTCCTCT CTGGTGGTCT CACGGTCCCT GTCACCCACT 
CACTTCCTGT CAGGGACTTC ATCCCCACCT CTCCACCCCA TTCCTCCACC CACTTGCCTG 
AGTTACCCCC ATCTTCCACC CCCCTCACCC ACAAAGCCCT TTGCCTCAAA ACCTCTCCCA 
TACTGGACCA AGTATGATGT TGCAGATTGG CTGGGCTACC TGAACCTGGG TGAACACAGG 
GAGCGATTCT TAGACAATGA AATTGATGGC ACGCACCTGC CCTCTCTTAC CAAGGATGAC 
TACCTGGACC TGGGTGTGAC CCGAGTTGGC CATCGCATGA ACATAGAGAG GGCGCTCAGG 
AGAGTTATGG ACAGGCTGTC TTCTAGCACT TTCCCCATTT CTGCTCTCTC TCCTCGAAAT 
GGACGGACAA ACAGAAGGAG AGATGAGGCC AACCGGAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329756.1
CDS	227..5548
Translation	MIDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMVDRASAPWKKKSEFESSQATEKKRTVYQMALNKLDEILAAAQQTINTNEAPGPRVQGVKRERGRGFYNEPNFDQTGMGMTSSGAGLGYDRGQFVSGHGPPHGMLRQKSIGVPEEEKQFLHPPAMKFARSLSVPGPDEIPPPPTTAPPEPPFSSAPPLGFRGKTSQQPSVSMSQSTSSAAHYQLYSQSVHVTHGPRDRSPGPPTGSGTGDLAYSHAQAHPSVPSRTASRKVIGYTGDLAGAGGVAGAKAAALRRGYSNAVPPTSVATVIPQQQQTTQNQPSRMDRSGMGVGAGGVPKGGARRGKGPLVKQSKVEDLRQGQDTLEAKVSVEKSSIPIPTIIVKAPSTSSSGRSSQGSSVEAEPPPPGESDSSKPEPVVPPPAPTVPPPAPPSIPAPPPPSLPSLRTQESLDFTSQFGAAIVGAARRDRERFHEARRKSASFFLSAEEELSGAGGAGGRTQTSQQQLSSQPSPRLRPSKSIDEGMFSGDTFIHHTRSMPPAFGLPEYSSPAPQGDYQPKSVPADFYGAGSRQQQQQQQQQAPTTTFIHPLTGKVLDPSSPLGLALAARERALKDDGRIRRERTTEHHFTRQLSSIGAFSSSTSQPTSHISYPMASSSSATSSAAQSTNTSSYLVTSSSLAATTTSTRPPSPRILRGAGSSWSEEAGVGERADREAAGTSAPREGLRVRFSEDKTVHTHHYQSQQYQPTFKERERERQRERSREREREREREREIEKAQTQYQQQSSQPMTQQPRRPSFLRMESEAGAYILSLTPPSPPPTAAPMRPTAGATGESGSGTSLMVLPPPAPSVDADDEFVFADPLPPPLEFANSFDRGLGYSAMISHNVAARQQRHPPPPPPPPPPPTSKDSFMTGFPAHTPLPSPQAGDSTTSSLTSYDSEVANLTQSAPSPSPSSPNPLPPHSPSTLQTSGPPPPPSVSPPPPPNSYHRPFGLSQSQHLYNSTHTTGRSSSPTPPLSIPAPPAYRGPPTASSTPATSVSIRGASTVTASCSTTASTTVTSPSTTAAATPHTRIATVSTVATGGSRRPSAEHHPPPSATKSGESQETVVDSGIEELDSRSSSDHHLDSILALSGSGVRERLERAGMSSIGGIGGRDGATEVDRTSGADFLESYMSYLDGQTFEMHNAKAASTASTYPKTQRYREGTRAGDLRRQTSTAPPAYHRRREEEEEEDEAEEVEEEDLSARDGYGMREGPGIARSSVMYFDRPRTPEIKPLWGDGSTGASQTMGEGGGQIVGTYVEPRKLHPPMTGMKANIINELSTKLQQRSKGTENWGAQRSLSRHRHRFSEDSTGALSPPSVRSSSPSPVQLSQPALSPSPTPSSQPLYSNWARSPSPQPPAASSQPPPRSHSPVSPSSYSPYPTSPKHRPVYRTKALEFQFIPSRESRKSESHHAQRRRAPSPLISPSDRPKLGPPRPSSLPILPTTPLYSSLYDLRGSITPPSPANPLGDPYFSPSPPLFAPSGAPPPPNSSLVVSRSLSPTHFLSGTSSPPLHPIPPPTCLSYPHLPPPSPTKPFASKPLPYWTKYDVADWLGYLNLGEHRERFLDNEIDGTHLPSLTKDDYLDLGVTRVGHRMNIERALRRVMDRLSSSTFPISALSPRNGRTNRRRDEANRS

Target ORF information:

RefSeq Version	XM_021474081.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474081.1

ORF Insert Sequence:

ATGATTGACT ACATAATCAA GGAGAAGACA GTCCTCCTGC AGAAAAAAGA CAATGAAGGT 
TTTGGCTTTG TGCTGAGAGG AGCCAAGGCT CAGACCCCTA TAGAAGAGTT CACTCCCACC 
CCAGCCTTTC CAGCACTGCA GTACCTTGAG TCTGTCGACG AGGGAGGCGT GGCCTGGAGA 
GCTGGTCTGC GAATGGGAGA CTTCCTTATA GAGGTGAATG GTCAGAATGT GGTTAAGGTG 
GGACACAGGC AGGTGGTCAA TATGATCAGG CAAGGCGGTA ACAGTCTCAT GGTCAAGGTT 
GTCATGGTTA CTCGAAATCC TGATATGGAG GAAGGGGTCA GAAAGAAAAT CCCACAGCAG 
AGTAAAAGAC TGAGCACACC GGCTATCGCT CTGCGCTCCA AATCCATGAC ATCAGAGCTG 
GAAGAGATGG TGGACAGAGC CTCTGCCCCA TGGAAAAAAA AATCAGAGTT CGAATCTTCA 
CAAGCTACAG AGAAGAAGAG GACAGTTTAT CAGATGGCTT TGAATAAACT GGATGAGATT 
TTAGCAGCAG CACAGCAGAC CATTAATACA AATGAGGCTC CAGGGCCCCG GGTTCAGGGG 
GTAAAAAGAG AACGGGGCAG AGGCTTTTAT AATGAGCCAA ATTTCGACCA AACTGGAATG 
GGGATGACAT CATCTGGGGC AGGGTTAGGC TACGACCGTG GTCAATTTGT GTCTGGCCAT 
GGCCCCCCGC ACGGTATGCT GCGACAGAAG TCTATCGGGG TTCCTGAGGA GGAGAAGCAG 
TTTCTTCATC CTCCAGCCAT GAAGTTTGCC CGTAGTCTTT CAGTACCTGG CCCAGATGAA 
ATCCCTCCTC CACCTACTAC AGCACCCCCT GAGCCCCCTT TTTCCTCAGC TCCTCCGCTT 
GGCTTTAGGG GCAAGACATC TCAACAGCCG TCTGTTTCTA TGTCTCAGTC TACTTCCTCT 
GCTGCACATT ATCAACTCTA CTCACAGTCA GTACATGTTA CTCACGGACC AAGGGACAGG 
TCCCCTGGAC CACCTACTGG GTCTGGAACT GGGGACCTTG CTTATTCCCA TGCCCAAGCC 
CACCCCTCTG TACCAAGTAG GACTGCCTCC AGGAAGGTGA TTGGGTACAC AGGAGATCTT 
GCAGGGGCTG GAGGAGTGGC TGGGGCCAAA GCTGCAGCAT TGAGAAGGGG TTACAGCAAT 
GCAGTCCCAC CCACCAGTGT CGCTACTGTG ATTCCACAAC AGCAGCAGAC TACTCAGAAC 
CAACCTTCCA GAATGGACCG GAGTGGAATG GGGGTAGGGG CTGGTGGTGT ACCAAAAGGC 
GGTGCTCGTA GAGGAAAGGG GCCCTTGGTG AAACAGTCAA AGGTGGAAGA CCTGCGTCAA 
GGTCAAGACA CTCTAGAAGC AAAGGTGTCA GTGGAGAAGA GCTCTATTCC AATCCCAACT 
ATCATTGTTA AAGCCCCTTC CACCAGCAGT AGTGGACGCA GTAGCCAAGG CAGCAGTGTG 
GAGGCTGAAC CACCGCCCCC AGGAGAAAGT GACAGTTCTA AACCAGAACC TGTTGTTCCC 
CCACCTGCAC CAACCGTGCC ACCACCAGCC CCTCCATCTA TACCTGCTCC TCCACCCCCA 
TCACTGCCAT CACTTCGTAC TCAAGAGAGT CTGGACTTCA CCAGCCAGTT TGGTGCTGCC 
ATTGTAGGGG CAGCTCGCAG GGACAGAGAA CGATTTCATG AAGCACGACG CAAGAGTGCC 
TCCTTTTTTC TTTCTGCTGA GGAAGAGTTG AGTGGAGCTG GAGGAGCAGG AGGGCGGACT 
CAGACTTCTC AACAGCAGCT GAGTTCCCAG CCTTCACCAC GCCTGCGACC ATCCAAATCT 
ATAGATGAAG GCATGTTCTC AGGTGATACT TTTATCCATC ACACACGCAG TATGCCTCCT 
GCATTTGGCT TGCCTGAGTA CTCCTCACCT GCTCCTCAAG GGGATTACCA GCCTAAGTCT 
GTACCTGCTG ACTTCTATGG GGCAGGATCC AGACAACAGC AGCAGCAGCA GCAGCAGCAA 
GCCCCCACCA CAACTTTTAT TCACCCACTA ACTGGAAAAG TACTGGACCC TTCATCCCCT 
CTAGGCTTGG CTTTGGCTGC ACGGGAACGT GCCTTGAAGG ATGATGGACG TATACGTAGG 
GAAAGGACCA CTGAACATCA TTTTACCCGT CAGTTGTCAA GCATTGGGGC CTTTTCCTCA 
TCCACTTCAC AGCCAACATC ACACATTTCC TACCCAATGG CCTCGTCTTC CTCTGCAACC 
TCCTCTGCTG CTCAATCCAC AAACACCTCT TCATATCTTG TTACCTCCTC ATCATTAGCT 
GCCACCACCA CATCCACTCG GCCTCCATCT CCTAGGATCC TGCGTGGAGC AGGGAGTAGC 
TGGAGTGAAG AAGCTGGGGT TGGAGAGCGT GCGGATAGGG AAGCAGCTGG CACCTCTGCT 
CCCAGAGAAG GCCTAAGGGT TCGTTTCTCC GAGGATAAAA CTGTCCACAC ACACCACTAT 
CAATCTCAGC AATATCAGCC CACCTTTAAA GAGCGTGAAA GAGAACGTCA AAGAGAGAGG 
TCAAGAGAAA GAGAGAGGGA AAGAGAGAGG GAAAGAGAAA TAGAAAAAGC TCAAACCCAG 
TATCAGCAAC AGTCATCACA ACCAATGACC CAGCAGCCTC GACGCCCCTC ATTCTTGAGG 
ATGGAGAGTG AAGCTGGAGC GTACATTCTT TCATTAACGC CTCCATCTCC TCCTCCAACA 
GCAGCCCCTA TGCGTCCTAC AGCAGGAGCC ACAGGTGAAA GTGGAAGTGG TACAAGCCTG 
ATGGTTCTGC CTCCCCCAGC TCCCTCAGTA GATGCAGATG ATGAGTTTGT GTTTGCTGAT 
CCTCTTCCTC CACCTCTAGA GTTTGCCAAT AGTTTTGATC GTGGTTTGGG TTACTCTGCG 
ATGATTTCTC ACAATGTTGC AGCCCGTCAG CAGCGTCATC CTCCACCACC CCCTCCCCCA 
CCACCCCCGC CCACTTCGAA AGATTCCTTT ATGACAGGCT TTCCAGCTCA CACACCCCTA 
CCGTCACCTC AGGCAGGAGA TTCCACAACT TCCAGTCTTA CCTCCTATGA CAGTGAGGTT 
GCAAACCTAA CACAATCTGC CCCATCGCCC TCCCCATCTT CTCCCAACCC ACTACCACCT 
CACTCCCCCT CCACCCTTCA GACATCTGGA CCACCTCCAC CTCCCTCTGT ATCACCTCCT 
CCACCACCCA ACAGCTACCA TAGACCTTTT GGCTTATCTC AGTCACAACA TTTATACAAC 
AGTACACACA CAACTGGACG CTCCTCCTCT CCCACCCCAC CTCTCTCTAT TCCAGCTCCA 
CCTGCCTACC GGGGGCCACC AACAGCATCA TCCACCCCTG CAACTTCTGT ATCAATCAGA 
GGTGCCTCAA CTGTGACCGC AAGCTGTTCC ACAACTGCTT CTACAACTGT CACCTCTCCT 
AGTACTACTG CTGCTGCCAC ACCACACACA CGCATAGCAA CTGTCTCCAC AGTAGCTACA 
GGGGGTAGTA GGCGTCCCAG TGCAGAGCAT CATCCACCAC CTAGTGCAAC CAAGAGTGGT 
GAGTCCCAGG AGACAGTGGT AGATTCTGGG ATTGAGGAGC TGGACAGCCG AAGCAGCAGT 
GATCATCACT TAGACAGCAT TCTTGCACTC AGTGGTAGTG GGGTCAGAGA GAGGTTGGAA 
CGAGCAGGGA TGAGTAGCAT AGGTGGAATT GGAGGGAGAG ATGGTGCAAC TGAGGTTGAC 
AGGACAAGTG GGGCAGACTT TTTAGAGTCT TACATGAGCT ACCTTGATGG GCAGACTTTT 
GAAATGCACA ATGCCAAAGC CGCTAGCACT GCATCCACCT ACCCAAAAAC ACAGAGGTAC 
AGAGAAGGCA CCCGGGCTGG AGATCTTCGA CGCCAGACCA GCACAGCACC TCCTGCATAC 
CACCGTCGTA GAGAGGAGGA AGAGGAAGAG GATGAGGCCG AGGAGGTAGA GGAGGAGGAT 
TTGAGTGCCC GTGATGGCTA TGGGATGAGG GAAGGGCCAG GGATTGCTCG CTCCTCTGTA 
ATGTACTTTG ACCGGCCCCG CACTCCAGAG ATAAAACCAC TGTGGGGAGA TGGGTCGACT 
GGAGCATCAC AGACCATGGG GGAGGGAGGG GGCCAGATAG TGGGAACATA TGTGGAGCCA 
CGTAAACTAC ATCCTCCCAT GACTGGTATG AAAGCAAACA TCATTAATGA ACTAAGCACC 
AAATTACAGC AGAGAAGTAA AGGAACAGAG AACTGGGGGG CCCAGAGATC ATTGAGCAGA 
CATAGACACA GGTTCTCAGA GGACTCAACA GGTGCACTGA GTCCTCCCTC TGTCCGCTCT 
AGCTCCCCAT CTCCAGTTCA GCTCTCTCAG CCTGCCTTGT CTCCCTCTCC CACTCCTTCC 
TCCCAGCCAC TTTACTCAAA CTGGGCTCGA TCTCCTTCCC CTCAGCCCCC TGCTGCCTCA 
TCCCAGCCCC CTCCCCGATC CCATTCACCT GTTTCCCCTT CATCATATTC ACCATACCCC 
ACCTCACCCA AACACCGGCC TGTTTATCGC ACCAAAGCTC TGGAGTTCCA ATTTATTCCC 
AGTCGAGAGT CACGAAAGTC AGAGTCGCAT CATGCCCAGC GCAGACGAGC ACCCAGCCCT 
TTAATTTCTC CATCTGACCG TCCCAAGCTT GGCCCTCCCC GCCCCTCTTC CCTTCCTATT 
CTCCCCACTA CACCCCTCTA TAGCAGCCTG TATGACCTTC GTGGTTCTAT TACTCCACCC 
TCACCTGCCA ATCCCCTTGG GGACCCCTAC TTTTCCCCCT CCCCCCCTCT ATTTGCCCCC 
TCGGGTGCTC CCCCTCCTCC AAATTCCTCT CTGGTGGTCT CACGGTCCCT GTCACCCACT 
CACTTCCTGT CAGGGACTTC ATCCCCACCT CTCCACCCCA TTCCTCCACC CACTTGCCTG 
AGTTACCCCC ATCTTCCACC CCCCTCACCC ACAAAGCCCT TTGCCTCAAA ACCTCTCCCA 
TACTGGACCA AGTATGATGT TGCAGATTGG CTGGGCTACC TGAACCTGGG TGAACACAGG 
GAGCGATTCT TAGACAATGA AATTGATGGC ACGCACCTGC CCTCTCTTAC CAAGGATGAC 
TACCTGGACC TGGGTGTGAC CCGAGTTGGC CATCGCATGA ACATAGAGAG GGCGCTCAGG 
AGAGTTATGG ACAGGCTGTC TTCTAGCACT TTCCCCATTT CTGCTCTCTC TCCTCGAAAT 
GGACGGACAA ACAGAAGGAG AGATGAGGCC AACCGGAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_021471437.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327112.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X7, mRNA.

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CloneID	ODa141769
Clone ID Related Accession (Same CDS sequence)	XM_021474081.1 , XM_021471437.1
Accession Version	XM_021471437.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394533.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGACT ACATAATCAA GGAGAAGACA GTCCTCCTGC AGAAAAAAGA CAATGAAGGT 
TTTGGCTTTG TGCTGAGAGG AGCCAAGGCT CAGACCCCTA TAGAAGAGTT CACTCCCACC 
CCAGCCTTTC CAGCACTGCA GTACCTTGAG TCTGTCGACG AGGGAGGCGT GGCCTGGAGA 
GCTGGTCTGC GAATGGGAGA CTTCCTTATA GAGGTGAATG GTCAGAATGT GGTTAAGGTG 
GGACACAGGC AGGTGGTCAA TATGATCAGG CAAGGCGGTA ACAGTCTCAT GGTCAAGGTT 
GTCATGGTTA CTCGAAATCC TGATATGGAG GAAGGGGTCA GAAAGAAAAT CCCACAGCAG 
AGTAAAAGAC TGAGCACACC GGCTATCGCT CTGCGCTCCA AATCCATGAC ATCAGAGCTG 
GAAGAGATGG TGGACAGAGC CTCTGCCCCA TGGAAAAAAA AATCAGAGTT CGAATCTTCA 
CAAGCTACAG AGAAGAAGAG GACAGTTTAT CAGATGGCTT TGAATAAACT GGATGAGATT 
TTAGCAGCAG CACAGCAGAC CATTAATACA AATGAGGCTC CAGGGCCCCG GGTTCAGGGG 
GTAAAAAGAG AACGGGGCAG AGGCTTTTAT AATGAGCCAA ATTTCGACCA AACTGGAATG 
GGGATGACAT CATCTGGGGC AGGGTTAGGC TACGACCGTG GTCAATTTGT GTCTGGCCAT 
GGCCCCCCGC ACGGTATGCT GCGACAGAAG TCTATCGGGG TTCCTGAGGA GGAGAAGCAG 
TTTCTTCATC CTCCAGCCAT GAAGTTTGCC CGTAGTCTTT CAGTACCTGG CCCAGATGAA 
ATCCCTCCTC CACCTACTAC AGCACCCCCT GAGCCCCCTT TTTCCTCAGC TCCTCCGCTT 
GGCTTTAGGG GCAAGACATC TCAACAGCCG TCTGTTTCTA TGTCTCAGTC TACTTCCTCT 
GCTGCACATT ATCAACTCTA CTCACAGTCA GTACATGTTA CTCACGGACC AAGGGACAGG 
TCCCCTGGAC CACCTACTGG GTCTGGAACT GGGGACCTTG CTTATTCCCA TGCCCAAGCC 
CACCCCTCTG TACCAAGTAG GACTGCCTCC AGGAAGGTGA TTGGGTACAC AGGAGATCTT 
GCAGGGGCTG GAGGAGTGGC TGGGGCCAAA GCTGCAGCAT TGAGAAGGGG TTACAGCAAT 
GCAGTCCCAC CCACCAGTGT CGCTACTGTG ATTCCACAAC AGCAGCAGAC TACTCAGAAC 
CAACCTTCCA GAATGGACCG GAGTGGAATG GGGGTAGGGG CTGGTGGTGT ACCAAAAGGC 
GGTGCTCGTA GAGGAAAGGG GCCCTTGGTG AAACAGTCAA AGGTGGAAGA CCTGCGTCAA 
GGTCAAGACA CTCTAGAAGC AAAGGTGTCA GTGGAGAAGA GCTCTATTCC AATCCCAACT 
ATCATTGTTA AAGCCCCTTC CACCAGCAGT AGTGGACGCA GTAGCCAAGG CAGCAGTGTG 
GAGGCTGAAC CACCGCCCCC AGGAGAAAGT GACAGTTCTA AACCAGAACC TGTTGTTCCC 
CCACCTGCAC CAACCGTGCC ACCACCAGCC CCTCCATCTA TACCTGCTCC TCCACCCCCA 
TCACTGCCAT CACTTCGTAC TCAAGAGAGT CTGGACTTCA CCAGCCAGTT TGGTGCTGCC 
ATTGTAGGGG CAGCTCGCAG GGACAGAGAA CGATTTCATG AAGCACGACG CAAGAGTGCC 
TCCTTTTTTC TTTCTGCTGA GGAAGAGTTG AGTGGAGCTG GAGGAGCAGG AGGGCGGACT 
CAGACTTCTC AACAGCAGCT GAGTTCCCAG CCTTCACCAC GCCTGCGACC ATCCAAATCT 
ATAGATGAAG GCATGTTCTC AGGTGATACT TTTATCCATC ACACACGCAG TATGCCTCCT 
GCATTTGGCT TGCCTGAGTA CTCCTCACCT GCTCCTCAAG GGGATTACCA GCCTAAGTCT 
GTACCTGCTG ACTTCTATGG GGCAGGATCC AGACAACAGC AGCAGCAGCA GCAGCAGCAA 
GCCCCCACCA CAACTTTTAT TCACCCACTA ACTGGAAAAG TACTGGACCC TTCATCCCCT 
CTAGGCTTGG CTTTGGCTGC ACGGGAACGT GCCTTGAAGG ATGATGGACG TATACGTAGG 
GAAAGGACCA CTGAACATCA TTTTACCCGT CAGTTGTCAA GCATTGGGGC CTTTTCCTCA 
TCCACTTCAC AGCCAACATC ACACATTTCC TACCCAATGG CCTCGTCTTC CTCTGCAACC 
TCCTCTGCTG CTCAATCCAC AAACACCTCT TCATATCTTG TTACCTCCTC ATCATTAGCT 
GCCACCACCA CATCCACTCG GCCTCCATCT CCTAGGATCC TGCGTGGAGC AGGGAGTAGC 
TGGAGTGAAG AAGCTGGGGT TGGAGAGCGT GCGGATAGGG AAGCAGCTGG CACCTCTGCT 
CCCAGAGAAG GCCTAAGGGT TCGTTTCTCC GAGGATAAAA CTGTCCACAC ACACCACTAT 
CAATCTCAGC AATATCAGCC CACCTTTAAA GAGCGTGAAA GAGAACGTCA AAGAGAGAGG 
TCAAGAGAAA GAGAGAGGGA AAGAGAGAGG GAAAGAGAAA TAGAAAAAGC TCAAACCCAG 
TATCAGCAAC AGTCATCACA ACCAATGACC CAGCAGCCTC GACGCCCCTC ATTCTTGAGG 
ATGGAGAGTG AAGCTGGAGC GTACATTCTT TCATTAACGC CTCCATCTCC TCCTCCAACA 
GCAGCCCCTA TGCGTCCTAC AGCAGGAGCC ACAGGTGAAA GTGGAAGTGG TACAAGCCTG 
ATGGTTCTGC CTCCCCCAGC TCCCTCAGTA GATGCAGATG ATGAGTTTGT GTTTGCTGAT 
CCTCTTCCTC CACCTCTAGA GTTTGCCAAT AGTTTTGATC GTGGTTTGGG TTACTCTGCG 
ATGATTTCTC ACAATGTTGC AGCCCGTCAG CAGCGTCATC CTCCACCACC CCCTCCCCCA 
CCACCCCCGC CCACTTCGAA AGATTCCTTT ATGACAGGCT TTCCAGCTCA CACACCCCTA 
CCGTCACCTC AGGCAGGAGA TTCCACAACT TCCAGTCTTA CCTCCTATGA CAGTGAGGTT 
GCAAACCTAA CACAATCTGC CCCATCGCCC TCCCCATCTT CTCCCAACCC ACTACCACCT 
CACTCCCCCT CCACCCTTCA GACATCTGGA CCACCTCCAC CTCCCTCTGT ATCACCTCCT 
CCACCACCCA ACAGCTACCA TAGACCTTTT GGCTTATCTC AGTCACAACA TTTATACAAC 
AGTACACACA CAACTGGACG CTCCTCCTCT CCCACCCCAC CTCTCTCTAT TCCAGCTCCA 
CCTGCCTACC GGGGGCCACC AACAGCATCA TCCACCCCTG CAACTTCTGT ATCAATCAGA 
GGTGCCTCAA CTGTGACCGC AAGCTGTTCC ACAACTGCTT CTACAACTGT CACCTCTCCT 
AGTACTACTG CTGCTGCCAC ACCACACACA CGCATAGCAA CTGTCTCCAC AGTAGCTACA 
GGGGGTAGTA GGCGTCCCAG TGCAGAGCAT CATCCACCAC CTAGTGCAAC CAAGAGTGGT 
GAGTCCCAGG AGACAGTGGT AGATTCTGGG ATTGAGGAGC TGGACAGCCG AAGCAGCAGT 
GATCATCACT TAGACAGCAT TCTTGCACTC AGTGGTAGTG GGGTCAGAGA GAGGTTGGAA 
CGAGCAGGGA TGAGTAGCAT AGGTGGAATT GGAGGGAGAG ATGGTGCAAC TGAGGTTGAC 
AGGACAAGTG GGGCAGACTT TTTAGAGTCT TACATGAGCT ACCTTGATGG GCAGACTTTT 
GAAATGCACA ATGCCAAAGC CGCTAGCACT GCATCCACCT ACCCAAAAAC ACAGAGGTAC 
AGAGAAGGCA CCCGGGCTGG AGATCTTCGA CGCCAGACCA GCACAGCACC TCCTGCATAC 
CACCGTCGTA GAGAGGAGGA AGAGGAAGAG GATGAGGCCG AGGAGGTAGA GGAGGAGGAT 
TTGAGTGCCC GTGATGGCTA TGGGATGAGG GAAGGGCCAG GGATTGCTCG CTCCTCTGTA 
ATGTACTTTG ACCGGCCCCG CACTCCAGAG ATAAAACCAC TGTGGGGAGA TGGGTCGACT 
GGAGCATCAC AGACCATGGG GGAGGGAGGG GGCCAGATAG TGGGAACATA TGTGGAGCCA 
CGTAAACTAC ATCCTCCCAT GACTGGTATG AAAGCAAACA TCATTAATGA ACTAAGCACC 
AAATTACAGC AGAGAAGTAA AGGAACAGAG AACTGGGGGG CCCAGAGATC ATTGAGCAGA 
CATAGACACA GGTTCTCAGA GGACTCAACA GGTGCACTGA GTCCTCCCTC TGTCCGCTCT 
AGCTCCCCAT CTCCAGTTCA GCTCTCTCAG CCTGCCTTGT CTCCCTCTCC CACTCCTTCC 
TCCCAGCCAC TTTACTCAAA CTGGGCTCGA TCTCCTTCCC CTCAGCCCCC TGCTGCCTCA 
TCCCAGCCCC CTCCCCGATC CCATTCACCT GTTTCCCCTT CATCATATTC ACCATACCCC 
ACCTCACCCA AACACCGGCC TGTTTATCGC ACCAAAGCTC TGGAGTTCCA ATTTATTCCC 
AGTCGAGAGT CACGAAAGTC AGAGTCGCAT CATGCCCAGC GCAGACGAGC ACCCAGCCCT 
TTAATTTCTC CATCTGACCG TCCCAAGCTT GGCCCTCCCC GCCCCTCTTC CCTTCCTATT 
CTCCCCACTA CACCCCTCTA TAGCAGCCTG TATGACCTTC GTGGTTCTAT TACTCCACCC 
TCACCTGCCA ATCCCCTTGG GGACCCCTAC TTTTCCCCCT CCCCCCCTCT ATTTGCCCCC 
TCGGGTGCTC CCCCTCCTCC AAATTCCTCT CTGGTGGTCT CACGGTCCCT GTCACCCACT 
CACTTCCTGT CAGGGACTTC ATCCCCACCT CTCCACCCCA TTCCTCCACC CACTTGCCTG 
AGTTACCCCC ATCTTCCACC CCCCTCACCC ACAAAGCCCT TTGCCTCAAA ACCTCTCCCA 
TACTGGACCA AGTATGATGT TGCAGATTGG CTGGGCTACC TGAACCTGGG TGAACACAGG 
GAGCGATTCT TAGACAATGA AATTGATGGC ACGCACCTGC CCTCTCTTAC CAAGGATGAC 
TACCTGGACC TGGGTGTGAC CCGAGTTGGC CATCGCATGA ACATAGAGAG GGCGCTCAGG 
AGAGTTATGG ACAGGCTGTC TTCTAGCACT TTCCCCATTT CTGCTCTCTC TCCTCGAAAT 
GGACGGACAA ACAGAAGGAG AGATGAGGCC AACCGGAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327112.1
CDS	227..5548
Translation	MIDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMVDRASAPWKKKSEFESSQATEKKRTVYQMALNKLDEILAAAQQTINTNEAPGPRVQGVKRERGRGFYNEPNFDQTGMGMTSSGAGLGYDRGQFVSGHGPPHGMLRQKSIGVPEEEKQFLHPPAMKFARSLSVPGPDEIPPPPTTAPPEPPFSSAPPLGFRGKTSQQPSVSMSQSTSSAAHYQLYSQSVHVTHGPRDRSPGPPTGSGTGDLAYSHAQAHPSVPSRTASRKVIGYTGDLAGAGGVAGAKAAALRRGYSNAVPPTSVATVIPQQQQTTQNQPSRMDRSGMGVGAGGVPKGGARRGKGPLVKQSKVEDLRQGQDTLEAKVSVEKSSIPIPTIIVKAPSTSSSGRSSQGSSVEAEPPPPGESDSSKPEPVVPPPAPTVPPPAPPSIPAPPPPSLPSLRTQESLDFTSQFGAAIVGAARRDRERFHEARRKSASFFLSAEEELSGAGGAGGRTQTSQQQLSSQPSPRLRPSKSIDEGMFSGDTFIHHTRSMPPAFGLPEYSSPAPQGDYQPKSVPADFYGAGSRQQQQQQQQQAPTTTFIHPLTGKVLDPSSPLGLALAARERALKDDGRIRRERTTEHHFTRQLSSIGAFSSSTSQPTSHISYPMASSSSATSSAAQSTNTSSYLVTSSSLAATTTSTRPPSPRILRGAGSSWSEEAGVGERADREAAGTSAPREGLRVRFSEDKTVHTHHYQSQQYQPTFKERERERQRERSREREREREREREIEKAQTQYQQQSSQPMTQQPRRPSFLRMESEAGAYILSLTPPSPPPTAAPMRPTAGATGESGSGTSLMVLPPPAPSVDADDEFVFADPLPPPLEFANSFDRGLGYSAMISHNVAARQQRHPPPPPPPPPPPTSKDSFMTGFPAHTPLPSPQAGDSTTSSLTSYDSEVANLTQSAPSPSPSSPNPLPPHSPSTLQTSGPPPPPSVSPPPPPNSYHRPFGLSQSQHLYNSTHTTGRSSSPTPPLSIPAPPAYRGPPTASSTPATSVSIRGASTVTASCSTTASTTVTSPSTTAAATPHTRIATVSTVATGGSRRPSAEHHPPPSATKSGESQETVVDSGIEELDSRSSSDHHLDSILALSGSGVRERLERAGMSSIGGIGGRDGATEVDRTSGADFLESYMSYLDGQTFEMHNAKAASTASTYPKTQRYREGTRAGDLRRQTSTAPPAYHRRREEEEEEDEAEEVEEEDLSARDGYGMREGPGIARSSVMYFDRPRTPEIKPLWGDGSTGASQTMGEGGGQIVGTYVEPRKLHPPMTGMKANIINELSTKLQQRSKGTENWGAQRSLSRHRHRFSEDSTGALSPPSVRSSSPSPVQLSQPALSPSPTPSSQPLYSNWARSPSPQPPAASSQPPPRSHSPVSPSSYSPYPTSPKHRPVYRTKALEFQFIPSRESRKSESHHAQRRRAPSPLISPSDRPKLGPPRPSSLPILPTTPLYSSLYDLRGSITPPSPANPLGDPYFSPSPPLFAPSGAPPPPNSSLVVSRSLSPTHFLSGTSSPPLHPIPPPTCLSYPHLPPPSPTKPFASKPLPYWTKYDVADWLGYLNLGEHRERFLDNEIDGTHLPSLTKDDYLDLGVTRVGHRMNIERALRRVMDRLSSSTFPISALSPRNGRTNRRRDEANRS

Target ORF information:

RefSeq Version	XM_021471437.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471437.1

ORF Insert Sequence:

ATGATTGACT ACATAATCAA GGAGAAGACA GTCCTCCTGC AGAAAAAAGA CAATGAAGGT 
TTTGGCTTTG TGCTGAGAGG AGCCAAGGCT CAGACCCCTA TAGAAGAGTT CACTCCCACC 
CCAGCCTTTC CAGCACTGCA GTACCTTGAG TCTGTCGACG AGGGAGGCGT GGCCTGGAGA 
GCTGGTCTGC GAATGGGAGA CTTCCTTATA GAGGTGAATG GTCAGAATGT GGTTAAGGTG 
GGACACAGGC AGGTGGTCAA TATGATCAGG CAAGGCGGTA ACAGTCTCAT GGTCAAGGTT 
GTCATGGTTA CTCGAAATCC TGATATGGAG GAAGGGGTCA GAAAGAAAAT CCCACAGCAG 
AGTAAAAGAC TGAGCACACC GGCTATCGCT CTGCGCTCCA AATCCATGAC ATCAGAGCTG 
GAAGAGATGG TGGACAGAGC CTCTGCCCCA TGGAAAAAAA AATCAGAGTT CGAATCTTCA 
CAAGCTACAG AGAAGAAGAG GACAGTTTAT CAGATGGCTT TGAATAAACT GGATGAGATT 
TTAGCAGCAG CACAGCAGAC CATTAATACA AATGAGGCTC CAGGGCCCCG GGTTCAGGGG 
GTAAAAAGAG AACGGGGCAG AGGCTTTTAT AATGAGCCAA ATTTCGACCA AACTGGAATG 
GGGATGACAT CATCTGGGGC AGGGTTAGGC TACGACCGTG GTCAATTTGT GTCTGGCCAT 
GGCCCCCCGC ACGGTATGCT GCGACAGAAG TCTATCGGGG TTCCTGAGGA GGAGAAGCAG 
TTTCTTCATC CTCCAGCCAT GAAGTTTGCC CGTAGTCTTT CAGTACCTGG CCCAGATGAA 
ATCCCTCCTC CACCTACTAC AGCACCCCCT GAGCCCCCTT TTTCCTCAGC TCCTCCGCTT 
GGCTTTAGGG GCAAGACATC TCAACAGCCG TCTGTTTCTA TGTCTCAGTC TACTTCCTCT 
GCTGCACATT ATCAACTCTA CTCACAGTCA GTACATGTTA CTCACGGACC AAGGGACAGG 
TCCCCTGGAC CACCTACTGG GTCTGGAACT GGGGACCTTG CTTATTCCCA TGCCCAAGCC 
CACCCCTCTG TACCAAGTAG GACTGCCTCC AGGAAGGTGA TTGGGTACAC AGGAGATCTT 
GCAGGGGCTG GAGGAGTGGC TGGGGCCAAA GCTGCAGCAT TGAGAAGGGG TTACAGCAAT 
GCAGTCCCAC CCACCAGTGT CGCTACTGTG ATTCCACAAC AGCAGCAGAC TACTCAGAAC 
CAACCTTCCA GAATGGACCG GAGTGGAATG GGGGTAGGGG CTGGTGGTGT ACCAAAAGGC 
GGTGCTCGTA GAGGAAAGGG GCCCTTGGTG AAACAGTCAA AGGTGGAAGA CCTGCGTCAA 
GGTCAAGACA CTCTAGAAGC AAAGGTGTCA GTGGAGAAGA GCTCTATTCC AATCCCAACT 
ATCATTGTTA AAGCCCCTTC CACCAGCAGT AGTGGACGCA GTAGCCAAGG CAGCAGTGTG 
GAGGCTGAAC CACCGCCCCC AGGAGAAAGT GACAGTTCTA AACCAGAACC TGTTGTTCCC 
CCACCTGCAC CAACCGTGCC ACCACCAGCC CCTCCATCTA TACCTGCTCC TCCACCCCCA 
TCACTGCCAT CACTTCGTAC TCAAGAGAGT CTGGACTTCA CCAGCCAGTT TGGTGCTGCC 
ATTGTAGGGG CAGCTCGCAG GGACAGAGAA CGATTTCATG AAGCACGACG CAAGAGTGCC 
TCCTTTTTTC TTTCTGCTGA GGAAGAGTTG AGTGGAGCTG GAGGAGCAGG AGGGCGGACT 
CAGACTTCTC AACAGCAGCT GAGTTCCCAG CCTTCACCAC GCCTGCGACC ATCCAAATCT 
ATAGATGAAG GCATGTTCTC AGGTGATACT TTTATCCATC ACACACGCAG TATGCCTCCT 
GCATTTGGCT TGCCTGAGTA CTCCTCACCT GCTCCTCAAG GGGATTACCA GCCTAAGTCT 
GTACCTGCTG ACTTCTATGG GGCAGGATCC AGACAACAGC AGCAGCAGCA GCAGCAGCAA 
GCCCCCACCA CAACTTTTAT TCACCCACTA ACTGGAAAAG TACTGGACCC TTCATCCCCT 
CTAGGCTTGG CTTTGGCTGC ACGGGAACGT GCCTTGAAGG ATGATGGACG TATACGTAGG 
GAAAGGACCA CTGAACATCA TTTTACCCGT CAGTTGTCAA GCATTGGGGC CTTTTCCTCA 
TCCACTTCAC AGCCAACATC ACACATTTCC TACCCAATGG CCTCGTCTTC CTCTGCAACC 
TCCTCTGCTG CTCAATCCAC AAACACCTCT TCATATCTTG TTACCTCCTC ATCATTAGCT 
GCCACCACCA CATCCACTCG GCCTCCATCT CCTAGGATCC TGCGTGGAGC AGGGAGTAGC 
TGGAGTGAAG AAGCTGGGGT TGGAGAGCGT GCGGATAGGG AAGCAGCTGG CACCTCTGCT 
CCCAGAGAAG GCCTAAGGGT TCGTTTCTCC GAGGATAAAA CTGTCCACAC ACACCACTAT 
CAATCTCAGC AATATCAGCC CACCTTTAAA GAGCGTGAAA GAGAACGTCA AAGAGAGAGG 
TCAAGAGAAA GAGAGAGGGA AAGAGAGAGG GAAAGAGAAA TAGAAAAAGC TCAAACCCAG 
TATCAGCAAC AGTCATCACA ACCAATGACC CAGCAGCCTC GACGCCCCTC ATTCTTGAGG 
ATGGAGAGTG AAGCTGGAGC GTACATTCTT TCATTAACGC CTCCATCTCC TCCTCCAACA 
GCAGCCCCTA TGCGTCCTAC AGCAGGAGCC ACAGGTGAAA GTGGAAGTGG TACAAGCCTG 
ATGGTTCTGC CTCCCCCAGC TCCCTCAGTA GATGCAGATG ATGAGTTTGT GTTTGCTGAT 
CCTCTTCCTC CACCTCTAGA GTTTGCCAAT AGTTTTGATC GTGGTTTGGG TTACTCTGCG 
ATGATTTCTC ACAATGTTGC AGCCCGTCAG CAGCGTCATC CTCCACCACC CCCTCCCCCA 
CCACCCCCGC CCACTTCGAA AGATTCCTTT ATGACAGGCT TTCCAGCTCA CACACCCCTA 
CCGTCACCTC AGGCAGGAGA TTCCACAACT TCCAGTCTTA CCTCCTATGA CAGTGAGGTT 
GCAAACCTAA CACAATCTGC CCCATCGCCC TCCCCATCTT CTCCCAACCC ACTACCACCT 
CACTCCCCCT CCACCCTTCA GACATCTGGA CCACCTCCAC CTCCCTCTGT ATCACCTCCT 
CCACCACCCA ACAGCTACCA TAGACCTTTT GGCTTATCTC AGTCACAACA TTTATACAAC 
AGTACACACA CAACTGGACG CTCCTCCTCT CCCACCCCAC CTCTCTCTAT TCCAGCTCCA 
CCTGCCTACC GGGGGCCACC AACAGCATCA TCCACCCCTG CAACTTCTGT ATCAATCAGA 
GGTGCCTCAA CTGTGACCGC AAGCTGTTCC ACAACTGCTT CTACAACTGT CACCTCTCCT 
AGTACTACTG CTGCTGCCAC ACCACACACA CGCATAGCAA CTGTCTCCAC AGTAGCTACA 
GGGGGTAGTA GGCGTCCCAG TGCAGAGCAT CATCCACCAC CTAGTGCAAC CAAGAGTGGT 
GAGTCCCAGG AGACAGTGGT AGATTCTGGG ATTGAGGAGC TGGACAGCCG AAGCAGCAGT 
GATCATCACT TAGACAGCAT TCTTGCACTC AGTGGTAGTG GGGTCAGAGA GAGGTTGGAA 
CGAGCAGGGA TGAGTAGCAT AGGTGGAATT GGAGGGAGAG ATGGTGCAAC TGAGGTTGAC 
AGGACAAGTG GGGCAGACTT TTTAGAGTCT TACATGAGCT ACCTTGATGG GCAGACTTTT 
GAAATGCACA ATGCCAAAGC CGCTAGCACT GCATCCACCT ACCCAAAAAC ACAGAGGTAC 
AGAGAAGGCA CCCGGGCTGG AGATCTTCGA CGCCAGACCA GCACAGCACC TCCTGCATAC 
CACCGTCGTA GAGAGGAGGA AGAGGAAGAG GATGAGGCCG AGGAGGTAGA GGAGGAGGAT 
TTGAGTGCCC GTGATGGCTA TGGGATGAGG GAAGGGCCAG GGATTGCTCG CTCCTCTGTA 
ATGTACTTTG ACCGGCCCCG CACTCCAGAG ATAAAACCAC TGTGGGGAGA TGGGTCGACT 
GGAGCATCAC AGACCATGGG GGAGGGAGGG GGCCAGATAG TGGGAACATA TGTGGAGCCA 
CGTAAACTAC ATCCTCCCAT GACTGGTATG AAAGCAAACA TCATTAATGA ACTAAGCACC 
AAATTACAGC AGAGAAGTAA AGGAACAGAG AACTGGGGGG CCCAGAGATC ATTGAGCAGA 
CATAGACACA GGTTCTCAGA GGACTCAACA GGTGCACTGA GTCCTCCCTC TGTCCGCTCT 
AGCTCCCCAT CTCCAGTTCA GCTCTCTCAG CCTGCCTTGT CTCCCTCTCC CACTCCTTCC 
TCCCAGCCAC TTTACTCAAA CTGGGCTCGA TCTCCTTCCC CTCAGCCCCC TGCTGCCTCA 
TCCCAGCCCC CTCCCCGATC CCATTCACCT GTTTCCCCTT CATCATATTC ACCATACCCC 
ACCTCACCCA AACACCGGCC TGTTTATCGC ACCAAAGCTC TGGAGTTCCA ATTTATTCCC 
AGTCGAGAGT CACGAAAGTC AGAGTCGCAT CATGCCCAGC GCAGACGAGC ACCCAGCCCT 
TTAATTTCTC CATCTGACCG TCCCAAGCTT GGCCCTCCCC GCCCCTCTTC CCTTCCTATT 
CTCCCCACTA CACCCCTCTA TAGCAGCCTG TATGACCTTC GTGGTTCTAT TACTCCACCC 
TCACCTGCCA ATCCCCTTGG GGACCCCTAC TTTTCCCCCT CCCCCCCTCT ATTTGCCCCC 
TCGGGTGCTC CCCCTCCTCC AAATTCCTCT CTGGTGGTCT CACGGTCCCT GTCACCCACT 
CACTTCCTGT CAGGGACTTC ATCCCCACCT CTCCACCCCA TTCCTCCACC CACTTGCCTG 
AGTTACCCCC ATCTTCCACC CCCCTCACCC ACAAAGCCCT TTGCCTCAAA ACCTCTCCCA 
TACTGGACCA AGTATGATGT TGCAGATTGG CTGGGCTACC TGAACCTGGG TGAACACAGG 
GAGCGATTCT TAGACAATGA AATTGATGGC ACGCACCTGC CCTCTCTTAC CAAGGATGAC 
TACCTGGACC TGGGTGTGAC CCGAGTTGGC CATCGCATGA ACATAGAGAG GGCGCTCAGG 
AGAGTTATGG ACAGGCTGTC TTCTAGCACT TTCCCCATTT CTGCTCTCTC TCCTCGAAAT 
GGACGGACAA ACAGAAGGAG AGATGAGGCC AACCGGAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_021471431.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327106.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X1, mRNA.

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CloneID	ODa141764
Clone ID Related Accession (Same CDS sequence)	XM_021471431.1 , XM_021474051.1 , XM_021474058.1 , XM_021471432.1
Accession Version	XM_021471431.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394533.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGTG 
GACAGAGCCT CTGCCCCATG GAAAAAAAAA TCAGAGTTCG AATCTTCACA AGCTACAGAG 
AAGAAGAGGA CAGTTTATCA GATGGCTTTG AATAAACTGG ATGAGATTTT AGCAGCAGCA 
CAGCAGACCA TTAATACAAA TGAGGCTCCA GGGCCCCGGG TTCAGGGGGT AAAAAGAGAA 
CGGGGCAGAG GCTTTTATAA TGAGCCAAAT TTCGACCAAA CTGGAATGGG GATGACATCA 
TCTGGGGCAG GGTTAGGCTA CGACCGTGGT CAATTTGTGT CTGGCCATGG CCCCCCGCAC 
GGTATGCTGC GACAGAAGTC TATCGGGGTT CCTGAGGAGG AGAAGCAGTT TCTTCATCCT 
CCAGCCATGA AGTTTGCCCG TAGTCTTTCA GTACCTGGCC CAGATGAAAT CCCTCCTCCA 
CCTACTACAG CACCCCCTGA GCCCCCTTTT TCCTCAGCTC CTCCGCTTGG CTTTAGGGGC 
AAGACATCTC AACAGCCGTC TGTTTCTATG TCTCAGTCTA CTTCCTCTGC TGCACATTAT 
CAACTCTACT CACAGTCAGT ACATGTTACT CACGGACCAA GGGACAGGTC CCCTGGACCA 
CCTACTGGGT CTGGAACTGG GGACCTTGCT TATTCCCATG CCCAAGCCCA CCCCTCTGTA 
CCAAGTAGGA CTGCCTCCAG GAAGGTGATT GGGTACACAG GAGATCTTGC AGGGGCTGGA 
GGAGTGGCTG GGGCCAAAGC TGCAGCATTG AGAAGGGGTT ACAGCAATGC AGTCCCACCC 
ACCAGTGTCG CTACTGTGAT TCCACAACAG CAGCAGACTA CTCAGAACCA ACCTTCCAGA 
ATGGACCGGA GTGGAATGGG GGTAGGGGCT GGTGGTGTAC CAAAAGGCGG TGCTCGTAGA 
GGAAAGGGGC CCTTGGTGAA ACAGTCAAAG GTGGAAGACC TGCGTCAAGG TCAAGACACT 
CTAGAAGCAA AGGTGTCAGT GGAGAAGAGC TCTATTCCAA TCCCAACTAT CATTGTTAAA 
GCCCCTTCCA CCAGCAGTAG TGGACGCAGT AGCCAAGGCA GCAGTGTGGA GGCTGAACCA 
CCGCCCCCAG GAGAAAGTGA CAGTTCTAAA CCAGAACCTG TTGTTCCCCC ACCTGCACCA 
ACCGTGCCAC CACCAGCCCC TCCATCTATA CCTGCTCCTC CACCCCCATC ACTGCCATCA 
CTTCGTACTC AAGAGAGTCT GGACTTCACC AGCCAGTTTG GTGCTGCCAT TGTAGGGGCA 
GCTCGCAGGG ACAGAGAACG ATTTCATGAA GCACGACGCA AGAGTGCCTC CTTTTTTCTT 
TCTGCTGAGG AAGAGTTGAG TGGAGCTGGA GGAGCAGGAG GGCGGACTCA GACTTCTCAA 
CAGCAGCTGA GTTCCCAGCC TTCACCACGC CTGCGACCAT CCAAATCTAT AGATGAAGGC 
ATGTTCTCAG GTGATACTTT TATCCATCAC ACACGCAGTA TGCCTCCTGC ATTTGGCTTG 
CCTGAGTACT CCTCACCTGC TCCTCAAGGG GATTACCAGC CTAAGTCTGT ACCTGCTGAC 
TTCTATGGGG CAGGATCCAG ACAACAGCAG CAGCAGCAGC AGCAGCAAGC CCCCACCACA 
ACTTTTATTC ACCCACTAAC TGGAAAAGTA CTGGACCCTT CATCCCCTCT AGGCTTGGCT 
TTGGCTGCAC GGGAACGTGC CTTGAAGGAT GATGGACGTA TACGTAGGGA AAGGACCACT 
GAACATCATT TTACCCGTCA GTTGTCAAGC ATTGGGGCCT TTTCCTCATC CACTTCACAG 
CCAACATCAC ACATTTCCTA CCCAATGGCC TCGTCTTCCT CTGCAACCTC CTCTGCTGCT 
CAATCCACAA ACACCTCTTC ATATCTTGTT ACCTCCTCAT CATTAGCTGC CACCACCACA 
TCCACTCGGC CTCCATCTCC TAGGATCCTG CGTGGAGCAG GGAGTAGCTG GAGTGAAGAA 
GCTGGGGTTG GAGAGCGTGC GGATAGGGAA GCAGCTGGCA CCTCTGCTCC CAGAGAAGGC 
CTAAGGGTTC GTTTCTCCGA GGATAAAACT GTCCACACAC ACCACTATCA ATCTCAGCAA 
TATCAGCCCA CCTTTAAAGA GCGTGAAAGA GAACGTCAAA GAGAGAGGTC AAGAGAAAGA 
GAGAGGGAAA GAGAGAGGGA AAGAGAAATA GAAAAAGCTC AAACCCAGTA TCAGCAACAG 
TCATCACAAC CAATGACCCA GCAGCCTCGA CGCCCCTCAT TCTTGAGGAT GGAGAGTGAA 
GCTGGAGCGT ACATTCTTTC ATTAACGCCT CCATCTCCTC CTCCAACAGC AGCCCCTATG 
CGTCCTACAG CAGGAGCCAC AGGTGAAAGT GGAAGTGGTA CAAGCCTGAT GGTTCTGCCT 
CCCCCAGCTC CCTCAGTAGA TGCAGATGAT GAGTTTGTGT TTGCTGATCC TCTTCCTCCA 
CCTCTAGAGT TTGCCAATAG TTTTGATCGT GGTTTGGGTT ACTCTGCGAT GATTTCTCAC 
AATGTTGCAG CCCGTCAGCA GCGTCATCCT CCACCACCCC CTCCCCCACC ACCCCCGCCC 
ACTTCGAAAG ATTCCTTTAT GACAGGCTTT CCAGCTCACA CACCCCTACC GTCACCTCAG 
GCAGGAGATT CCACAACTTC CAGTCTTACC TCCTATGACA GTGAGGTTGC AAACCTAACA 
CAATCTGCCC CATCGCCCTC CCCATCTTCT CCCAACCCAC TACCACCTCA CTCCCCCTCC 
ACCCTTCAGA CATCTGGACC ACCTCCACCT CCCTCTGTAT CACCTCCTCC ACCACCCAAC 
AGCTACCATA GACCTTTTGG CTTATCTCAG TCACAACATT TATACAACAG TACACACACA 
ACTGGACGCT CCTCCTCTCC CACCCCACCT CTCTCTATTC CAGCTCCACC TGCCTACCGG 
GGGCCACCAA CAGCATCATC CACCCCTGCA ACTTCTGTAT CAATCAGAGG TGCCTCAACT 
GTGACCGCAA GCTGTTCCAC AACTGCTTCT ACAACTGTCA CCTCTCCTAG TACTACTGCT 
GCTGCCACAC CACACACACG CATAGCAACT GTCTCCACAG TAGCTACAGG GGGTAGTAGG 
CGTCCCAGTG CAGAGCATCA TCCACCACCT AGTGCAACCA AGAGTGGTGA GTCCCAGGAG 
ACAGTGGTAG ATTCTGGGAT TGAGGAGCTG GACAGCCGAA GCAGCAGTGA TCATCACTTA 
GACAGCATTC TTGCACTCAG TGGTAGTGGG GTCAGAGAGA GGTTGGAACG AGCAGGGATG 
AGTAGCATAG GTGGAATTGG AGGGAGAGAT GGTGCAACTG AGGTTGACAG GACAAGTGGG 
GCAGACTTTT TAGAGTCTTA CATGAGCTAC CTTGATGGGC AGACTTTTGA AATGCACAAT 
GCCAAAGCCG CTAGCACTGC ATCCACCTAC CCAAAAACAC AGAGGTACAG AGAAGGCACC 
CGGGCTGGAG ATCTTCGACG CCAGACCAGC ACAGCACCTC CTGCATACCA CCGTCGTAGA 
GAGGAGGAAG AGGAAGAGGA TGAGGCCGAG GAGGTAGAGG AGGAGGATTT GAGTGCCCGT 
GATGGCTATG GGATGAGGGA AGGGCCAGGG ATTGCTCGCT CCTCTGTAAT GTACTTTGAC 
CGGCCCCGCA CTCCAGAGAT AAAACCACTG TGGGGAGATG GGTCGACTGG AGCATCACAG 
ACCATGGGGG AGGGAGGGGG CCAGATAGTG GGAACATATG TGGAGCCACG TAAACTACAT 
CCTCCCATGA CTGGTATGAA AGCAAACATC ATTAATGAAC TAAGCACCAA ATTACAGCAG 
AGAAGTAAAG GAACAGAGAA CTGGGGGGCC CAGAGATCAT TGAGCAGACA TAGACACAGG 
TTCTCAGAGG ACTCAACAGG TGCACTGAGT CCTCCCTCTG TCCGCTCTAG CTCCCCATCT 
CCAGTTCAGC TCTCTCAGCC TGCCTTGTCT CCCTCTCCCA CTCCTTCCTC CCAGCCACTT 
TACTCAAACT GGGCTCGATC TCCTTCCCCT CAGCCCCCTG CTGCCTCATC CCAGCCCCCT 
CCCCGATCCC ATTCACCTGT TTCCCCTTCA TCATATTCAC CATACCCCAC CTCACCCAAA 
CACCGGCCTG TTTATCGCAC CAAAGCTCTG GAGTTCCAAT TTATTCCCAG TCGAGAGTCA 
CGAAAGTCAG AGTCGCATCA TGCCCAGCGC AGACGAGCAC CCAGCCCTTT AATTTCTCCA 
TCTGACCGTC CCAAGCTTGG CCCTCCCCGC CCCTCTTCCC TTCCTATTCT CCCCACTACA 
CCCCTCTATA GCAGCCTGTA TGACCTTCGT GGTTCTATTA CTCCACCCTC ACCTGCCAAT 
CCCCTTGGGG ACCCCTACTT TTCCCCCTCC CCCCCTCTAT TTGCCCCCTC GGGTGCTCCC 
CCTCCTCCAA ATTCCTCTCT GGTGGTCTCA CGGTCCCTGT CACCCACTCA CTTCCTGTCA 
GGGACTTCAT CCCCACCTCT CCACCCCATT CCTCCACCCA CTTGCCTGAG TTACCCCCAT 
CTTCCACCCC CCTCACCCAC AAAGCCCTTT GCCTCAAAAC CTCTCCCATA CTGGACCAAG 
TATGATGTTG CAGATTGGCT GGGCTACCTG AACCTGGGTG AACACAGGGA GCGATTCTTA 
GACAATGAAA TTGATGGCAC GCACCTGCCC TCTCTTACCA AGGATGACTA CCTGGACCTG 
GGTGTGACCC GAGTTGGCCA TCGCATGAAC ATAGAGAGGG CGCTCAGGAG AGTTATGGAC 
AGGCTGTCTT CTAGCACTTT CCCCATTTCT GCTCTCTCTC CTCGAAATGG ACGGACAAAC 
AGAAGGAGAG ATGAGGCCAA CCGGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327106.1
CDS	380..7789
Translation	MTMPLSPLSSDEEQQRMLGNSQHLYPGAEEEEEEEEEMDDDGMDEEGEEENGELEMDDEEEVDDEDDIRRGPGHLRGGREEGHRQKGTMAGRLPGDRPIRPGNPGHAANPYSSNPGPTHQTSTNQQQQQPQRKLRERSSSNAPDDTHVALMVFRIGIPDIKQTKCLRFNPDATVWKAKQQVMCSLTESLRDVLNYGLFQPATDGHDAKFLEEERPLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKASLKKFMDYIQTSAVEKVAKLIDKGLDPNYHDPDTGETPLTLAVQTEPGGSEIIRVLVMGGAHIDFRARDGLTPLHKAVRAHAHTALLTLLSLGASSDYKDSRGLTPLYHTVLIGGDTSCCETLLYHRARLGLRDENGWDETHQACQNGNSQHLEHLLFYGADSSSQNASGNTALHICALYNKESCARILLYRGAKKDTKNNSGQNSFQVAVMSGHFELGEIIKNHRESDVVPFLESPKYAPQRRESSRTLGLPHPHPFLRANSDNSMNVPDWIAFPNAAGSSIVSVQGLKHGGTLRSSSSPRGARTRSPSRGRTGDRDDRSRQTRGRQGAGSISSQGTASGQRRRLYSAVPGRVFVATRSHSAQGDREISFNKGDKVKVLSVGEGGYWEGTVRGRTGWFPSDCVEEVALRSLESRSESRSERAKRLFRHYTVGSYDSFDAPSDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMVDRASAPWKKKSEFESSQATEKKRTVYQMALNKLDEILAAAQQTINTNEAPGPRVQGVKRERGRGFYNEPNFDQTGMGMTSSGAGLGYDRGQFVSGHGPPHGMLRQKSIGVPEEEKQFLHPPAMKFARSLSVPGPDEIPPPPTTAPPEPPFSSAPPLGFRGKTSQQPSVSMSQSTSSAAHYQLYSQSVHVTHGPRDRSPGPPTGSGTGDLAYSHAQAHPSVPSRTASRKVIGYTGDLAGAGGVAGAKAAALRRGYSNAVPPTSVATVIPQQQQTTQNQPSRMDRSGMGVGAGGVPKGGARRGKGPLVKQSKVEDLRQGQDTLEAKVSVEKSSIPIPTIIVKAPSTSSSGRSSQGSSVEAEPPPPGESDSSKPEPVVPPPAPTVPPPAPPSIPAPPPPSLPSLRTQESLDFTSQFGAAIVGAARRDRERFHEARRKSASFFLSAEEELSGAGGAGGRTQTSQQQLSSQPSPRLRPSKSIDEGMFSGDTFIHHTRSMPPAFGLPEYSSPAPQGDYQPKSVPADFYGAGSRQQQQQQQQQAPTTTFIHPLTGKVLDPSSPLGLALAARERALKDDGRIRRERTTEHHFTRQLSSIGAFSSSTSQPTSHISYPMASSSSATSSAAQSTNTSSYLVTSSSLAATTTSTRPPSPRILRGAGSSWSEEAGVGERADREAAGTSAPREGLRVRFSEDKTVHTHHYQSQQYQPTFKERERERQRERSREREREREREREIEKAQTQYQQQSSQPMTQQPRRPSFLRMESEAGAYILSLTPPSPPPTAAPMRPTAGATGESGSGTSLMVLPPPAPSVDADDEFVFADPLPPPLEFANSFDRGLGYSAMISHNVAARQQRHPPPPPPPPPPPTSKDSFMTGFPAHTPLPSPQAGDSTTSSLTSYDSEVANLTQSAPSPSPSSPNPLPPHSPSTLQTSGPPPPPSVSPPPPPNSYHRPFGLSQSQHLYNSTHTTGRSSSPTPPLSIPAPPAYRGPPTASSTPATSVSIRGASTVTASCSTTASTTVTSPSTTAAATPHTRIATVSTVATGGSRRPSAEHHPPPSATKSGESQETVVDSGIEELDSRSSSDHHLDSILALSGSGVRERLERAGMSSIGGIGGRDGATEVDRTSGADFLESYMSYLDGQTFEMHNAKAASTASTYPKTQRYREGTRAGDLRRQTSTAPPAYHRRREEEEEEDEAEEVEEEDLSARDGYGMREGPGIARSSVMYFDRPRTPEIKPLWGDGSTGASQTMGEGGGQIVGTYVEPRKLHPPMTGMKANIINELSTKLQQRSKGTENWGAQRSLSRHRHRFSEDSTGALSPPSVRSSSPSPVQLSQPALSPSPTPSSQPLYSNWARSPSPQPPAASSQPPPRSHSPVSPSSYSPYPTSPKHRPVYRTKALEFQFIPSRESRKSESHHAQRRRAPSPLISPSDRPKLGPPRPSSLPILPTTPLYSSLYDLRGSITPPSPANPLGDPYFSPSPPLFAPSGAPPPPNSSLVVSRSLSPTHFLSGTSSPPLHPIPPPTCLSYPHLPPPSPTKPFASKPLPYWTKYDVADWLGYLNLGEHRERFLDNEIDGTHLPSLTKDDYLDLGVTRVGHRMNIERALRRVMDRLSSSTFPISALSPRNGRTNRRRDEANRS

Target ORF information:

RefSeq Version	XM_021471431.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471431.1

ORF Insert Sequence:

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGTG 
GACAGAGCCT CTGCCCCATG GAAAAAAAAA TCAGAGTTCG AATCTTCACA AGCTACAGAG 
AAGAAGAGGA CAGTTTATCA GATGGCTTTG AATAAACTGG ATGAGATTTT AGCAGCAGCA 
CAGCAGACCA TTAATACAAA TGAGGCTCCA GGGCCCCGGG TTCAGGGGGT AAAAAGAGAA 
CGGGGCAGAG GCTTTTATAA TGAGCCAAAT TTCGACCAAA CTGGAATGGG GATGACATCA 
TCTGGGGCAG GGTTAGGCTA CGACCGTGGT CAATTTGTGT CTGGCCATGG CCCCCCGCAC 
GGTATGCTGC GACAGAAGTC TATCGGGGTT CCTGAGGAGG AGAAGCAGTT TCTTCATCCT 
CCAGCCATGA AGTTTGCCCG TAGTCTTTCA GTACCTGGCC CAGATGAAAT CCCTCCTCCA 
CCTACTACAG CACCCCCTGA GCCCCCTTTT TCCTCAGCTC CTCCGCTTGG CTTTAGGGGC 
AAGACATCTC AACAGCCGTC TGTTTCTATG TCTCAGTCTA CTTCCTCTGC TGCACATTAT 
CAACTCTACT CACAGTCAGT ACATGTTACT CACGGACCAA GGGACAGGTC CCCTGGACCA 
CCTACTGGGT CTGGAACTGG GGACCTTGCT TATTCCCATG CCCAAGCCCA CCCCTCTGTA 
CCAAGTAGGA CTGCCTCCAG GAAGGTGATT GGGTACACAG GAGATCTTGC AGGGGCTGGA 
GGAGTGGCTG GGGCCAAAGC TGCAGCATTG AGAAGGGGTT ACAGCAATGC AGTCCCACCC 
ACCAGTGTCG CTACTGTGAT TCCACAACAG CAGCAGACTA CTCAGAACCA ACCTTCCAGA 
ATGGACCGGA GTGGAATGGG GGTAGGGGCT GGTGGTGTAC CAAAAGGCGG TGCTCGTAGA 
GGAAAGGGGC CCTTGGTGAA ACAGTCAAAG GTGGAAGACC TGCGTCAAGG TCAAGACACT 
CTAGAAGCAA AGGTGTCAGT GGAGAAGAGC TCTATTCCAA TCCCAACTAT CATTGTTAAA 
GCCCCTTCCA CCAGCAGTAG TGGACGCAGT AGCCAAGGCA GCAGTGTGGA GGCTGAACCA 
CCGCCCCCAG GAGAAAGTGA CAGTTCTAAA CCAGAACCTG TTGTTCCCCC ACCTGCACCA 
ACCGTGCCAC CACCAGCCCC TCCATCTATA CCTGCTCCTC CACCCCCATC ACTGCCATCA 
CTTCGTACTC AAGAGAGTCT GGACTTCACC AGCCAGTTTG GTGCTGCCAT TGTAGGGGCA 
GCTCGCAGGG ACAGAGAACG ATTTCATGAA GCACGACGCA AGAGTGCCTC CTTTTTTCTT 
TCTGCTGAGG AAGAGTTGAG TGGAGCTGGA GGAGCAGGAG GGCGGACTCA GACTTCTCAA 
CAGCAGCTGA GTTCCCAGCC TTCACCACGC CTGCGACCAT CCAAATCTAT AGATGAAGGC 
ATGTTCTCAG GTGATACTTT TATCCATCAC ACACGCAGTA TGCCTCCTGC ATTTGGCTTG 
CCTGAGTACT CCTCACCTGC TCCTCAAGGG GATTACCAGC CTAAGTCTGT ACCTGCTGAC 
TTCTATGGGG CAGGATCCAG ACAACAGCAG CAGCAGCAGC AGCAGCAAGC CCCCACCACA 
ACTTTTATTC ACCCACTAAC TGGAAAAGTA CTGGACCCTT CATCCCCTCT AGGCTTGGCT 
TTGGCTGCAC GGGAACGTGC CTTGAAGGAT GATGGACGTA TACGTAGGGA AAGGACCACT 
GAACATCATT TTACCCGTCA GTTGTCAAGC ATTGGGGCCT TTTCCTCATC CACTTCACAG 
CCAACATCAC ACATTTCCTA CCCAATGGCC TCGTCTTCCT CTGCAACCTC CTCTGCTGCT 
CAATCCACAA ACACCTCTTC ATATCTTGTT ACCTCCTCAT CATTAGCTGC CACCACCACA 
TCCACTCGGC CTCCATCTCC TAGGATCCTG CGTGGAGCAG GGAGTAGCTG GAGTGAAGAA 
GCTGGGGTTG GAGAGCGTGC GGATAGGGAA GCAGCTGGCA CCTCTGCTCC CAGAGAAGGC 
CTAAGGGTTC GTTTCTCCGA GGATAAAACT GTCCACACAC ACCACTATCA ATCTCAGCAA 
TATCAGCCCA CCTTTAAAGA GCGTGAAAGA GAACGTCAAA GAGAGAGGTC AAGAGAAAGA 
GAGAGGGAAA GAGAGAGGGA AAGAGAAATA GAAAAAGCTC AAACCCAGTA TCAGCAACAG 
TCATCACAAC CAATGACCCA GCAGCCTCGA CGCCCCTCAT TCTTGAGGAT GGAGAGTGAA 
GCTGGAGCGT ACATTCTTTC ATTAACGCCT CCATCTCCTC CTCCAACAGC AGCCCCTATG 
CGTCCTACAG CAGGAGCCAC AGGTGAAAGT GGAAGTGGTA CAAGCCTGAT GGTTCTGCCT 
CCCCCAGCTC CCTCAGTAGA TGCAGATGAT GAGTTTGTGT TTGCTGATCC TCTTCCTCCA 
CCTCTAGAGT TTGCCAATAG TTTTGATCGT GGTTTGGGTT ACTCTGCGAT GATTTCTCAC 
AATGTTGCAG CCCGTCAGCA GCGTCATCCT CCACCACCCC CTCCCCCACC ACCCCCGCCC 
ACTTCGAAAG ATTCCTTTAT GACAGGCTTT CCAGCTCACA CACCCCTACC GTCACCTCAG 
GCAGGAGATT CCACAACTTC CAGTCTTACC TCCTATGACA GTGAGGTTGC AAACCTAACA 
CAATCTGCCC CATCGCCCTC CCCATCTTCT CCCAACCCAC TACCACCTCA CTCCCCCTCC 
ACCCTTCAGA CATCTGGACC ACCTCCACCT CCCTCTGTAT CACCTCCTCC ACCACCCAAC 
AGCTACCATA GACCTTTTGG CTTATCTCAG TCACAACATT TATACAACAG TACACACACA 
ACTGGACGCT CCTCCTCTCC CACCCCACCT CTCTCTATTC CAGCTCCACC TGCCTACCGG 
GGGCCACCAA CAGCATCATC CACCCCTGCA ACTTCTGTAT CAATCAGAGG TGCCTCAACT 
GTGACCGCAA GCTGTTCCAC AACTGCTTCT ACAACTGTCA CCTCTCCTAG TACTACTGCT 
GCTGCCACAC CACACACACG CATAGCAACT GTCTCCACAG TAGCTACAGG GGGTAGTAGG 
CGTCCCAGTG CAGAGCATCA TCCACCACCT AGTGCAACCA AGAGTGGTGA GTCCCAGGAG 
ACAGTGGTAG ATTCTGGGAT TGAGGAGCTG GACAGCCGAA GCAGCAGTGA TCATCACTTA 
GACAGCATTC TTGCACTCAG TGGTAGTGGG GTCAGAGAGA GGTTGGAACG AGCAGGGATG 
AGTAGCATAG GTGGAATTGG AGGGAGAGAT GGTGCAACTG AGGTTGACAG GACAAGTGGG 
GCAGACTTTT TAGAGTCTTA CATGAGCTAC CTTGATGGGC AGACTTTTGA AATGCACAAT 
GCCAAAGCCG CTAGCACTGC ATCCACCTAC CCAAAAACAC AGAGGTACAG AGAAGGCACC 
CGGGCTGGAG ATCTTCGACG CCAGACCAGC ACAGCACCTC CTGCATACCA CCGTCGTAGA 
GAGGAGGAAG AGGAAGAGGA TGAGGCCGAG GAGGTAGAGG AGGAGGATTT GAGTGCCCGT 
GATGGCTATG GGATGAGGGA AGGGCCAGGG ATTGCTCGCT CCTCTGTAAT GTACTTTGAC 
CGGCCCCGCA CTCCAGAGAT AAAACCACTG TGGGGAGATG GGTCGACTGG AGCATCACAG 
ACCATGGGGG AGGGAGGGGG CCAGATAGTG GGAACATATG TGGAGCCACG TAAACTACAT 
CCTCCCATGA CTGGTATGAA AGCAAACATC ATTAATGAAC TAAGCACCAA ATTACAGCAG 
AGAAGTAAAG GAACAGAGAA CTGGGGGGCC CAGAGATCAT TGAGCAGACA TAGACACAGG 
TTCTCAGAGG ACTCAACAGG TGCACTGAGT CCTCCCTCTG TCCGCTCTAG CTCCCCATCT 
CCAGTTCAGC TCTCTCAGCC TGCCTTGTCT CCCTCTCCCA CTCCTTCCTC CCAGCCACTT 
TACTCAAACT GGGCTCGATC TCCTTCCCCT CAGCCCCCTG CTGCCTCATC CCAGCCCCCT 
CCCCGATCCC ATTCACCTGT TTCCCCTTCA TCATATTCAC CATACCCCAC CTCACCCAAA 
CACCGGCCTG TTTATCGCAC CAAAGCTCTG GAGTTCCAAT TTATTCCCAG TCGAGAGTCA 
CGAAAGTCAG AGTCGCATCA TGCCCAGCGC AGACGAGCAC CCAGCCCTTT AATTTCTCCA 
TCTGACCGTC CCAAGCTTGG CCCTCCCCGC CCCTCTTCCC TTCCTATTCT CCCCACTACA 
CCCCTCTATA GCAGCCTGTA TGACCTTCGT GGTTCTATTA CTCCACCCTC ACCTGCCAAT 
CCCCTTGGGG ACCCCTACTT TTCCCCCTCC CCCCCTCTAT TTGCCCCCTC GGGTGCTCCC 
CCTCCTCCAA ATTCCTCTCT GGTGGTCTCA CGGTCCCTGT CACCCACTCA CTTCCTGTCA 
GGGACTTCAT CCCCACCTCT CCACCCCATT CCTCCACCCA CTTGCCTGAG TTACCCCCAT 
CTTCCACCCC CCTCACCCAC AAAGCCCTTT GCCTCAAAAC CTCTCCCATA CTGGACCAAG 
TATGATGTTG CAGATTGGCT GGGCTACCTG AACCTGGGTG AACACAGGGA GCGATTCTTA 
GACAATGAAA TTGATGGCAC GCACCTGCCC TCTCTTACCA AGGATGACTA CCTGGACCTG 
GGTGTGACCC GAGTTGGCCA TCGCATGAAC ATAGAGAGGG CGCTCAGGAG AGTTATGGAC 
AGGCTGTCTT CTAGCACTTT CCCCATTTCT GCTCTCTCTC CTCGAAATGG ACGGACAAAC 
AGAAGGAGAG ATGAGGCCAA CCGGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_021474051.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329726.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X1, mRNA.

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CloneID	ODa141764
Clone ID Related Accession (Same CDS sequence)	XM_021471431.1 , XM_021474051.1 , XM_021474058.1 , XM_021471432.1
Accession Version	XM_021474051.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGTG 
GACAGAGCCT CTGCCCCATG GAAAAAAAAA TCAGAGTTCG AATCTTCACA AGCTACAGAG 
AAGAAGAGGA CAGTTTATCA GATGGCTTTG AATAAACTGG ATGAGATTTT AGCAGCAGCA 
CAGCAGACCA TTAATACAAA TGAGGCTCCA GGGCCCCGGG TTCAGGGGGT AAAAAGAGAA 
CGGGGCAGAG GCTTTTATAA TGAGCCAAAT TTCGACCAAA CTGGAATGGG GATGACATCA 
TCTGGGGCAG GGTTAGGCTA CGACCGTGGT CAATTTGTGT CTGGCCATGG CCCCCCGCAC 
GGTATGCTGC GACAGAAGTC TATCGGGGTT CCTGAGGAGG AGAAGCAGTT TCTTCATCCT 
CCAGCCATGA AGTTTGCCCG TAGTCTTTCA GTACCTGGCC CAGATGAAAT CCCTCCTCCA 
CCTACTACAG CACCCCCTGA GCCCCCTTTT TCCTCAGCTC CTCCGCTTGG CTTTAGGGGC 
AAGACATCTC AACAGCCGTC TGTTTCTATG TCTCAGTCTA CTTCCTCTGC TGCACATTAT 
CAACTCTACT CACAGTCAGT ACATGTTACT CACGGACCAA GGGACAGGTC CCCTGGACCA 
CCTACTGGGT CTGGAACTGG GGACCTTGCT TATTCCCATG CCCAAGCCCA CCCCTCTGTA 
CCAAGTAGGA CTGCCTCCAG GAAGGTGATT GGGTACACAG GAGATCTTGC AGGGGCTGGA 
GGAGTGGCTG GGGCCAAAGC TGCAGCATTG AGAAGGGGTT ACAGCAATGC AGTCCCACCC 
ACCAGTGTCG CTACTGTGAT TCCACAACAG CAGCAGACTA CTCAGAACCA ACCTTCCAGA 
ATGGACCGGA GTGGAATGGG GGTAGGGGCT GGTGGTGTAC CAAAAGGCGG TGCTCGTAGA 
GGAAAGGGGC CCTTGGTGAA ACAGTCAAAG GTGGAAGACC TGCGTCAAGG TCAAGACACT 
CTAGAAGCAA AGGTGTCAGT GGAGAAGAGC TCTATTCCAA TCCCAACTAT CATTGTTAAA 
GCCCCTTCCA CCAGCAGTAG TGGACGCAGT AGCCAAGGCA GCAGTGTGGA GGCTGAACCA 
CCGCCCCCAG GAGAAAGTGA CAGTTCTAAA CCAGAACCTG TTGTTCCCCC ACCTGCACCA 
ACCGTGCCAC CACCAGCCCC TCCATCTATA CCTGCTCCTC CACCCCCATC ACTGCCATCA 
CTTCGTACTC AAGAGAGTCT GGACTTCACC AGCCAGTTTG GTGCTGCCAT TGTAGGGGCA 
GCTCGCAGGG ACAGAGAACG ATTTCATGAA GCACGACGCA AGAGTGCCTC CTTTTTTCTT 
TCTGCTGAGG AAGAGTTGAG TGGAGCTGGA GGAGCAGGAG GGCGGACTCA GACTTCTCAA 
CAGCAGCTGA GTTCCCAGCC TTCACCACGC CTGCGACCAT CCAAATCTAT AGATGAAGGC 
ATGTTCTCAG GTGATACTTT TATCCATCAC ACACGCAGTA TGCCTCCTGC ATTTGGCTTG 
CCTGAGTACT CCTCACCTGC TCCTCAAGGG GATTACCAGC CTAAGTCTGT ACCTGCTGAC 
TTCTATGGGG CAGGATCCAG ACAACAGCAG CAGCAGCAGC AGCAGCAAGC CCCCACCACA 
ACTTTTATTC ACCCACTAAC TGGAAAAGTA CTGGACCCTT CATCCCCTCT AGGCTTGGCT 
TTGGCTGCAC GGGAACGTGC CTTGAAGGAT GATGGACGTA TACGTAGGGA AAGGACCACT 
GAACATCATT TTACCCGTCA GTTGTCAAGC ATTGGGGCCT TTTCCTCATC CACTTCACAG 
CCAACATCAC ACATTTCCTA CCCAATGGCC TCGTCTTCCT CTGCAACCTC CTCTGCTGCT 
CAATCCACAA ACACCTCTTC ATATCTTGTT ACCTCCTCAT CATTAGCTGC CACCACCACA 
TCCACTCGGC CTCCATCTCC TAGGATCCTG CGTGGAGCAG GGAGTAGCTG GAGTGAAGAA 
GCTGGGGTTG GAGAGCGTGC GGATAGGGAA GCAGCTGGCA CCTCTGCTCC CAGAGAAGGC 
CTAAGGGTTC GTTTCTCCGA GGATAAAACT GTCCACACAC ACCACTATCA ATCTCAGCAA 
TATCAGCCCA CCTTTAAAGA GCGTGAAAGA GAACGTCAAA GAGAGAGGTC AAGAGAAAGA 
GAGAGGGAAA GAGAGAGGGA AAGAGAAATA GAAAAAGCTC AAACCCAGTA TCAGCAACAG 
TCATCACAAC CAATGACCCA GCAGCCTCGA CGCCCCTCAT TCTTGAGGAT GGAGAGTGAA 
GCTGGAGCGT ACATTCTTTC ATTAACGCCT CCATCTCCTC CTCCAACAGC AGCCCCTATG 
CGTCCTACAG CAGGAGCCAC AGGTGAAAGT GGAAGTGGTA CAAGCCTGAT GGTTCTGCCT 
CCCCCAGCTC CCTCAGTAGA TGCAGATGAT GAGTTTGTGT TTGCTGATCC TCTTCCTCCA 
CCTCTAGAGT TTGCCAATAG TTTTGATCGT GGTTTGGGTT ACTCTGCGAT GATTTCTCAC 
AATGTTGCAG CCCGTCAGCA GCGTCATCCT CCACCACCCC CTCCCCCACC ACCCCCGCCC 
ACTTCGAAAG ATTCCTTTAT GACAGGCTTT CCAGCTCACA CACCCCTACC GTCACCTCAG 
GCAGGAGATT CCACAACTTC CAGTCTTACC TCCTATGACA GTGAGGTTGC AAACCTAACA 
CAATCTGCCC CATCGCCCTC CCCATCTTCT CCCAACCCAC TACCACCTCA CTCCCCCTCC 
ACCCTTCAGA CATCTGGACC ACCTCCACCT CCCTCTGTAT CACCTCCTCC ACCACCCAAC 
AGCTACCATA GACCTTTTGG CTTATCTCAG TCACAACATT TATACAACAG TACACACACA 
ACTGGACGCT CCTCCTCTCC CACCCCACCT CTCTCTATTC CAGCTCCACC TGCCTACCGG 
GGGCCACCAA CAGCATCATC CACCCCTGCA ACTTCTGTAT CAATCAGAGG TGCCTCAACT 
GTGACCGCAA GCTGTTCCAC AACTGCTTCT ACAACTGTCA CCTCTCCTAG TACTACTGCT 
GCTGCCACAC CACACACACG CATAGCAACT GTCTCCACAG TAGCTACAGG GGGTAGTAGG 
CGTCCCAGTG CAGAGCATCA TCCACCACCT AGTGCAACCA AGAGTGGTGA GTCCCAGGAG 
ACAGTGGTAG ATTCTGGGAT TGAGGAGCTG GACAGCCGAA GCAGCAGTGA TCATCACTTA 
GACAGCATTC TTGCACTCAG TGGTAGTGGG GTCAGAGAGA GGTTGGAACG AGCAGGGATG 
AGTAGCATAG GTGGAATTGG AGGGAGAGAT GGTGCAACTG AGGTTGACAG GACAAGTGGG 
GCAGACTTTT TAGAGTCTTA CATGAGCTAC CTTGATGGGC AGACTTTTGA AATGCACAAT 
GCCAAAGCCG CTAGCACTGC ATCCACCTAC CCAAAAACAC AGAGGTACAG AGAAGGCACC 
CGGGCTGGAG ATCTTCGACG CCAGACCAGC ACAGCACCTC CTGCATACCA CCGTCGTAGA 
GAGGAGGAAG AGGAAGAGGA TGAGGCCGAG GAGGTAGAGG AGGAGGATTT GAGTGCCCGT 
GATGGCTATG GGATGAGGGA AGGGCCAGGG ATTGCTCGCT CCTCTGTAAT GTACTTTGAC 
CGGCCCCGCA CTCCAGAGAT AAAACCACTG TGGGGAGATG GGTCGACTGG AGCATCACAG 
ACCATGGGGG AGGGAGGGGG CCAGATAGTG GGAACATATG TGGAGCCACG TAAACTACAT 
CCTCCCATGA CTGGTATGAA AGCAAACATC ATTAATGAAC TAAGCACCAA ATTACAGCAG 
AGAAGTAAAG GAACAGAGAA CTGGGGGGCC CAGAGATCAT TGAGCAGACA TAGACACAGG 
TTCTCAGAGG ACTCAACAGG TGCACTGAGT CCTCCCTCTG TCCGCTCTAG CTCCCCATCT 
CCAGTTCAGC TCTCTCAGCC TGCCTTGTCT CCCTCTCCCA CTCCTTCCTC CCAGCCACTT 
TACTCAAACT GGGCTCGATC TCCTTCCCCT CAGCCCCCTG CTGCCTCATC CCAGCCCCCT 
CCCCGATCCC ATTCACCTGT TTCCCCTTCA TCATATTCAC CATACCCCAC CTCACCCAAA 
CACCGGCCTG TTTATCGCAC CAAAGCTCTG GAGTTCCAAT TTATTCCCAG TCGAGAGTCA 
CGAAAGTCAG AGTCGCATCA TGCCCAGCGC AGACGAGCAC CCAGCCCTTT AATTTCTCCA 
TCTGACCGTC CCAAGCTTGG CCCTCCCCGC CCCTCTTCCC TTCCTATTCT CCCCACTACA 
CCCCTCTATA GCAGCCTGTA TGACCTTCGT GGTTCTATTA CTCCACCCTC ACCTGCCAAT 
CCCCTTGGGG ACCCCTACTT TTCCCCCTCC CCCCCTCTAT TTGCCCCCTC GGGTGCTCCC 
CCTCCTCCAA ATTCCTCTCT GGTGGTCTCA CGGTCCCTGT CACCCACTCA CTTCCTGTCA 
GGGACTTCAT CCCCACCTCT CCACCCCATT CCTCCACCCA CTTGCCTGAG TTACCCCCAT 
CTTCCACCCC CCTCACCCAC AAAGCCCTTT GCCTCAAAAC CTCTCCCATA CTGGACCAAG 
TATGATGTTG CAGATTGGCT GGGCTACCTG AACCTGGGTG AACACAGGGA GCGATTCTTA 
GACAATGAAA TTGATGGCAC GCACCTGCCC TCTCTTACCA AGGATGACTA CCTGGACCTG 
GGTGTGACCC GAGTTGGCCA TCGCATGAAC ATAGAGAGGG CGCTCAGGAG AGTTATGGAC 
AGGCTGTCTT CTAGCACTTT CCCCATTTCT GCTCTCTCTC CTCGAAATGG ACGGACAAAC 
AGAAGGAGAG ATGAGGCCAA CCGGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329726.1
CDS	380..7789
Translation	MTMPLSPLSSDEEQQRMLGNSQHLYPGAEEEEEEEEEMDDDGMDEEGEEENGELEMDDEEEVDDEDDIRRGPGHLRGGREEGHRQKGTMAGRLPGDRPIRPGNPGHAANPYSSNPGPTHQTSTNQQQQQPQRKLRERSSSNAPDDTHVALMVFRIGIPDIKQTKCLRFNPDATVWKAKQQVMCSLTESLRDVLNYGLFQPATDGHDAKFLEEERPLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKASLKKFMDYIQTSAVEKVAKLIDKGLDPNYHDPDTGETPLTLAVQTEPGGSEIIRVLVMGGAHIDFRARDGLTPLHKAVRAHAHTALLTLLSLGASSDYKDSRGLTPLYHTVLIGGDTSCCETLLYHRARLGLRDENGWDETHQACQNGNSQHLEHLLFYGADSSSQNASGNTALHICALYNKESCARILLYRGAKKDTKNNSGQNSFQVAVMSGHFELGEIIKNHRESDVVPFLESPKYAPQRRESSRTLGLPHPHPFLRANSDNSMNVPDWIAFPNAAGSSIVSVQGLKHGGTLRSSSSPRGARTRSPSRGRTGDRDDRSRQTRGRQGAGSISSQGTASGQRRRLYSAVPGRVFVATRSHSAQGDREISFNKGDKVKVLSVGEGGYWEGTVRGRTGWFPSDCVEEVALRSLESRSESRSERAKRLFRHYTVGSYDSFDAPSDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMVDRASAPWKKKSEFESSQATEKKRTVYQMALNKLDEILAAAQQTINTNEAPGPRVQGVKRERGRGFYNEPNFDQTGMGMTSSGAGLGYDRGQFVSGHGPPHGMLRQKSIGVPEEEKQFLHPPAMKFARSLSVPGPDEIPPPPTTAPPEPPFSSAPPLGFRGKTSQQPSVSMSQSTSSAAHYQLYSQSVHVTHGPRDRSPGPPTGSGTGDLAYSHAQAHPSVPSRTASRKVIGYTGDLAGAGGVAGAKAAALRRGYSNAVPPTSVATVIPQQQQTTQNQPSRMDRSGMGVGAGGVPKGGARRGKGPLVKQSKVEDLRQGQDTLEAKVSVEKSSIPIPTIIVKAPSTSSSGRSSQGSSVEAEPPPPGESDSSKPEPVVPPPAPTVPPPAPPSIPAPPPPSLPSLRTQESLDFTSQFGAAIVGAARRDRERFHEARRKSASFFLSAEEELSGAGGAGGRTQTSQQQLSSQPSPRLRPSKSIDEGMFSGDTFIHHTRSMPPAFGLPEYSSPAPQGDYQPKSVPADFYGAGSRQQQQQQQQQAPTTTFIHPLTGKVLDPSSPLGLALAARERALKDDGRIRRERTTEHHFTRQLSSIGAFSSSTSQPTSHISYPMASSSSATSSAAQSTNTSSYLVTSSSLAATTTSTRPPSPRILRGAGSSWSEEAGVGERADREAAGTSAPREGLRVRFSEDKTVHTHHYQSQQYQPTFKERERERQRERSREREREREREREIEKAQTQYQQQSSQPMTQQPRRPSFLRMESEAGAYILSLTPPSPPPTAAPMRPTAGATGESGSGTSLMVLPPPAPSVDADDEFVFADPLPPPLEFANSFDRGLGYSAMISHNVAARQQRHPPPPPPPPPPPTSKDSFMTGFPAHTPLPSPQAGDSTTSSLTSYDSEVANLTQSAPSPSPSSPNPLPPHSPSTLQTSGPPPPPSVSPPPPPNSYHRPFGLSQSQHLYNSTHTTGRSSSPTPPLSIPAPPAYRGPPTASSTPATSVSIRGASTVTASCSTTASTTVTSPSTTAAATPHTRIATVSTVATGGSRRPSAEHHPPPSATKSGESQETVVDSGIEELDSRSSSDHHLDSILALSGSGVRERLERAGMSSIGGIGGRDGATEVDRTSGADFLESYMSYLDGQTFEMHNAKAASTASTYPKTQRYREGTRAGDLRRQTSTAPPAYHRRREEEEEEDEAEEVEEEDLSARDGYGMREGPGIARSSVMYFDRPRTPEIKPLWGDGSTGASQTMGEGGGQIVGTYVEPRKLHPPMTGMKANIINELSTKLQQRSKGTENWGAQRSLSRHRHRFSEDSTGALSPPSVRSSSPSPVQLSQPALSPSPTPSSQPLYSNWARSPSPQPPAASSQPPPRSHSPVSPSSYSPYPTSPKHRPVYRTKALEFQFIPSRESRKSESHHAQRRRAPSPLISPSDRPKLGPPRPSSLPILPTTPLYSSLYDLRGSITPPSPANPLGDPYFSPSPPLFAPSGAPPPPNSSLVVSRSLSPTHFLSGTSSPPLHPIPPPTCLSYPHLPPPSPTKPFASKPLPYWTKYDVADWLGYLNLGEHRERFLDNEIDGTHLPSLTKDDYLDLGVTRVGHRMNIERALRRVMDRLSSSTFPISALSPRNGRTNRRRDEANRS

Target ORF information:

RefSeq Version	XM_021474051.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474051.1

ORF Insert Sequence:

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGTG 
GACAGAGCCT CTGCCCCATG GAAAAAAAAA TCAGAGTTCG AATCTTCACA AGCTACAGAG 
AAGAAGAGGA CAGTTTATCA GATGGCTTTG AATAAACTGG ATGAGATTTT AGCAGCAGCA 
CAGCAGACCA TTAATACAAA TGAGGCTCCA GGGCCCCGGG TTCAGGGGGT AAAAAGAGAA 
CGGGGCAGAG GCTTTTATAA TGAGCCAAAT TTCGACCAAA CTGGAATGGG GATGACATCA 
TCTGGGGCAG GGTTAGGCTA CGACCGTGGT CAATTTGTGT CTGGCCATGG CCCCCCGCAC 
GGTATGCTGC GACAGAAGTC TATCGGGGTT CCTGAGGAGG AGAAGCAGTT TCTTCATCCT 
CCAGCCATGA AGTTTGCCCG TAGTCTTTCA GTACCTGGCC CAGATGAAAT CCCTCCTCCA 
CCTACTACAG CACCCCCTGA GCCCCCTTTT TCCTCAGCTC CTCCGCTTGG CTTTAGGGGC 
AAGACATCTC AACAGCCGTC TGTTTCTATG TCTCAGTCTA CTTCCTCTGC TGCACATTAT 
CAACTCTACT CACAGTCAGT ACATGTTACT CACGGACCAA GGGACAGGTC CCCTGGACCA 
CCTACTGGGT CTGGAACTGG GGACCTTGCT TATTCCCATG CCCAAGCCCA CCCCTCTGTA 
CCAAGTAGGA CTGCCTCCAG GAAGGTGATT GGGTACACAG GAGATCTTGC AGGGGCTGGA 
GGAGTGGCTG GGGCCAAAGC TGCAGCATTG AGAAGGGGTT ACAGCAATGC AGTCCCACCC 
ACCAGTGTCG CTACTGTGAT TCCACAACAG CAGCAGACTA CTCAGAACCA ACCTTCCAGA 
ATGGACCGGA GTGGAATGGG GGTAGGGGCT GGTGGTGTAC CAAAAGGCGG TGCTCGTAGA 
GGAAAGGGGC CCTTGGTGAA ACAGTCAAAG GTGGAAGACC TGCGTCAAGG TCAAGACACT 
CTAGAAGCAA AGGTGTCAGT GGAGAAGAGC TCTATTCCAA TCCCAACTAT CATTGTTAAA 
GCCCCTTCCA CCAGCAGTAG TGGACGCAGT AGCCAAGGCA GCAGTGTGGA GGCTGAACCA 
CCGCCCCCAG GAGAAAGTGA CAGTTCTAAA CCAGAACCTG TTGTTCCCCC ACCTGCACCA 
ACCGTGCCAC CACCAGCCCC TCCATCTATA CCTGCTCCTC CACCCCCATC ACTGCCATCA 
CTTCGTACTC AAGAGAGTCT GGACTTCACC AGCCAGTTTG GTGCTGCCAT TGTAGGGGCA 
GCTCGCAGGG ACAGAGAACG ATTTCATGAA GCACGACGCA AGAGTGCCTC CTTTTTTCTT 
TCTGCTGAGG AAGAGTTGAG TGGAGCTGGA GGAGCAGGAG GGCGGACTCA GACTTCTCAA 
CAGCAGCTGA GTTCCCAGCC TTCACCACGC CTGCGACCAT CCAAATCTAT AGATGAAGGC 
ATGTTCTCAG GTGATACTTT TATCCATCAC ACACGCAGTA TGCCTCCTGC ATTTGGCTTG 
CCTGAGTACT CCTCACCTGC TCCTCAAGGG GATTACCAGC CTAAGTCTGT ACCTGCTGAC 
TTCTATGGGG CAGGATCCAG ACAACAGCAG CAGCAGCAGC AGCAGCAAGC CCCCACCACA 
ACTTTTATTC ACCCACTAAC TGGAAAAGTA CTGGACCCTT CATCCCCTCT AGGCTTGGCT 
TTGGCTGCAC GGGAACGTGC CTTGAAGGAT GATGGACGTA TACGTAGGGA AAGGACCACT 
GAACATCATT TTACCCGTCA GTTGTCAAGC ATTGGGGCCT TTTCCTCATC CACTTCACAG 
CCAACATCAC ACATTTCCTA CCCAATGGCC TCGTCTTCCT CTGCAACCTC CTCTGCTGCT 
CAATCCACAA ACACCTCTTC ATATCTTGTT ACCTCCTCAT CATTAGCTGC CACCACCACA 
TCCACTCGGC CTCCATCTCC TAGGATCCTG CGTGGAGCAG GGAGTAGCTG GAGTGAAGAA 
GCTGGGGTTG GAGAGCGTGC GGATAGGGAA GCAGCTGGCA CCTCTGCTCC CAGAGAAGGC 
CTAAGGGTTC GTTTCTCCGA GGATAAAACT GTCCACACAC ACCACTATCA ATCTCAGCAA 
TATCAGCCCA CCTTTAAAGA GCGTGAAAGA GAACGTCAAA GAGAGAGGTC AAGAGAAAGA 
GAGAGGGAAA GAGAGAGGGA AAGAGAAATA GAAAAAGCTC AAACCCAGTA TCAGCAACAG 
TCATCACAAC CAATGACCCA GCAGCCTCGA CGCCCCTCAT TCTTGAGGAT GGAGAGTGAA 
GCTGGAGCGT ACATTCTTTC ATTAACGCCT CCATCTCCTC CTCCAACAGC AGCCCCTATG 
CGTCCTACAG CAGGAGCCAC AGGTGAAAGT GGAAGTGGTA CAAGCCTGAT GGTTCTGCCT 
CCCCCAGCTC CCTCAGTAGA TGCAGATGAT GAGTTTGTGT TTGCTGATCC TCTTCCTCCA 
CCTCTAGAGT TTGCCAATAG TTTTGATCGT GGTTTGGGTT ACTCTGCGAT GATTTCTCAC 
AATGTTGCAG CCCGTCAGCA GCGTCATCCT CCACCACCCC CTCCCCCACC ACCCCCGCCC 
ACTTCGAAAG ATTCCTTTAT GACAGGCTTT CCAGCTCACA CACCCCTACC GTCACCTCAG 
GCAGGAGATT CCACAACTTC CAGTCTTACC TCCTATGACA GTGAGGTTGC AAACCTAACA 
CAATCTGCCC CATCGCCCTC CCCATCTTCT CCCAACCCAC TACCACCTCA CTCCCCCTCC 
ACCCTTCAGA CATCTGGACC ACCTCCACCT CCCTCTGTAT CACCTCCTCC ACCACCCAAC 
AGCTACCATA GACCTTTTGG CTTATCTCAG TCACAACATT TATACAACAG TACACACACA 
ACTGGACGCT CCTCCTCTCC CACCCCACCT CTCTCTATTC CAGCTCCACC TGCCTACCGG 
GGGCCACCAA CAGCATCATC CACCCCTGCA ACTTCTGTAT CAATCAGAGG TGCCTCAACT 
GTGACCGCAA GCTGTTCCAC AACTGCTTCT ACAACTGTCA CCTCTCCTAG TACTACTGCT 
GCTGCCACAC CACACACACG CATAGCAACT GTCTCCACAG TAGCTACAGG GGGTAGTAGG 
CGTCCCAGTG CAGAGCATCA TCCACCACCT AGTGCAACCA AGAGTGGTGA GTCCCAGGAG 
ACAGTGGTAG ATTCTGGGAT TGAGGAGCTG GACAGCCGAA GCAGCAGTGA TCATCACTTA 
GACAGCATTC TTGCACTCAG TGGTAGTGGG GTCAGAGAGA GGTTGGAACG AGCAGGGATG 
AGTAGCATAG GTGGAATTGG AGGGAGAGAT GGTGCAACTG AGGTTGACAG GACAAGTGGG 
GCAGACTTTT TAGAGTCTTA CATGAGCTAC CTTGATGGGC AGACTTTTGA AATGCACAAT 
GCCAAAGCCG CTAGCACTGC ATCCACCTAC CCAAAAACAC AGAGGTACAG AGAAGGCACC 
CGGGCTGGAG ATCTTCGACG CCAGACCAGC ACAGCACCTC CTGCATACCA CCGTCGTAGA 
GAGGAGGAAG AGGAAGAGGA TGAGGCCGAG GAGGTAGAGG AGGAGGATTT GAGTGCCCGT 
GATGGCTATG GGATGAGGGA AGGGCCAGGG ATTGCTCGCT CCTCTGTAAT GTACTTTGAC 
CGGCCCCGCA CTCCAGAGAT AAAACCACTG TGGGGAGATG GGTCGACTGG AGCATCACAG 
ACCATGGGGG AGGGAGGGGG CCAGATAGTG GGAACATATG TGGAGCCACG TAAACTACAT 
CCTCCCATGA CTGGTATGAA AGCAAACATC ATTAATGAAC TAAGCACCAA ATTACAGCAG 
AGAAGTAAAG GAACAGAGAA CTGGGGGGCC CAGAGATCAT TGAGCAGACA TAGACACAGG 
TTCTCAGAGG ACTCAACAGG TGCACTGAGT CCTCCCTCTG TCCGCTCTAG CTCCCCATCT 
CCAGTTCAGC TCTCTCAGCC TGCCTTGTCT CCCTCTCCCA CTCCTTCCTC CCAGCCACTT 
TACTCAAACT GGGCTCGATC TCCTTCCCCT CAGCCCCCTG CTGCCTCATC CCAGCCCCCT 
CCCCGATCCC ATTCACCTGT TTCCCCTTCA TCATATTCAC CATACCCCAC CTCACCCAAA 
CACCGGCCTG TTTATCGCAC CAAAGCTCTG GAGTTCCAAT TTATTCCCAG TCGAGAGTCA 
CGAAAGTCAG AGTCGCATCA TGCCCAGCGC AGACGAGCAC CCAGCCCTTT AATTTCTCCA 
TCTGACCGTC CCAAGCTTGG CCCTCCCCGC CCCTCTTCCC TTCCTATTCT CCCCACTACA 
CCCCTCTATA GCAGCCTGTA TGACCTTCGT GGTTCTATTA CTCCACCCTC ACCTGCCAAT 
CCCCTTGGGG ACCCCTACTT TTCCCCCTCC CCCCCTCTAT TTGCCCCCTC GGGTGCTCCC 
CCTCCTCCAA ATTCCTCTCT GGTGGTCTCA CGGTCCCTGT CACCCACTCA CTTCCTGTCA 
GGGACTTCAT CCCCACCTCT CCACCCCATT CCTCCACCCA CTTGCCTGAG TTACCCCCAT 
CTTCCACCCC CCTCACCCAC AAAGCCCTTT GCCTCAAAAC CTCTCCCATA CTGGACCAAG 
TATGATGTTG CAGATTGGCT GGGCTACCTG AACCTGGGTG AACACAGGGA GCGATTCTTA 
GACAATGAAA TTGATGGCAC GCACCTGCCC TCTCTTACCA AGGATGACTA CCTGGACCTG 
GGTGTGACCC GAGTTGGCCA TCGCATGAAC ATAGAGAGGG CGCTCAGGAG AGTTATGGAC 
AGGCTGTCTT CTAGCACTTT CCCCATTTCT GCTCTCTCTC CTCGAAATGG ACGGACAAAC 
AGAAGGAGAG ATGAGGCCAA CCGGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_021474058.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329733.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X2, mRNA.

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CloneID	ODa141764
Clone ID Related Accession (Same CDS sequence)	XM_021471431.1 , XM_021474051.1 , XM_021474058.1 , XM_021471432.1
Accession Version	XM_021474058.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGTG 
GACAGAGCCT CTGCCCCATG GAAAAAAAAA TCAGAGTTCG AATCTTCACA AGCTACAGAG 
AAGAAGAGGA CAGTTTATCA GATGGCTTTG AATAAACTGG ATGAGATTTT AGCAGCAGCA 
CAGCAGACCA TTAATACAAA TGAGGCTCCA GGGCCCCGGG TTCAGGGGGT AAAAAGAGAA 
CGGGGCAGAG GCTTTTATAA TGAGCCAAAT TTCGACCAAA CTGGAATGGG GATGACATCA 
TCTGGGGCAG GGTTAGGCTA CGACCGTGGT CAATTTGTGT CTGGCCATGG CCCCCCGCAC 
GGTATGCTGC GACAGAAGTC TATCGGGGTT CCTGAGGAGG AGAAGCAGTT TCTTCATCCT 
CCAGCCATGA AGTTTGCCCG TAGTCTTTCA GTACCTGGCC CAGATGAAAT CCCTCCTCCA 
CCTACTACAG CACCCCCTGA GCCCCCTTTT TCCTCAGCTC CTCCGCTTGG CTTTAGGGGC 
AAGACATCTC AACAGCCGTC TGTTTCTATG TCTCAGTCTA CTTCCTCTGC TGCACATTAT 
CAACTCTACT CACAGTCAGT ACATGTTACT CACGGACCAA GGGACAGGTC CCCTGGACCA 
CCTACTGGGT CTGGAACTGG GGACCTTGCT TATTCCCATG CCCAAGCCCA CCCCTCTGTA 
CCAAGTAGGA CTGCCTCCAG GAAGGTGATT GGGTACACAG GAGATCTTGC AGGGGCTGGA 
GGAGTGGCTG GGGCCAAAGC TGCAGCATTG AGAAGGGGTT ACAGCAATGC AGTCCCACCC 
ACCAGTGTCG CTACTGTGAT TCCACAACAG CAGCAGACTA CTCAGAACCA ACCTTCCAGA 
ATGGACCGGA GTGGAATGGG GGTAGGGGCT GGTGGTGTAC CAAAAGGCGG TGCTCGTAGA 
GGAAAGGGGC CCTTGGTGAA ACAGTCAAAG GTGGAAGACC TGCGTCAAGG TCAAGACACT 
CTAGAAGCAA AGGTGTCAGT GGAGAAGAGC TCTATTCCAA TCCCAACTAT CATTGTTAAA 
GCCCCTTCCA CCAGCAGTAG TGGACGCAGT AGCCAAGGCA GCAGTGTGGA GGCTGAACCA 
CCGCCCCCAG GAGAAAGTGA CAGTTCTAAA CCAGAACCTG TTGTTCCCCC ACCTGCACCA 
ACCGTGCCAC CACCAGCCCC TCCATCTATA CCTGCTCCTC CACCCCCATC ACTGCCATCA 
CTTCGTACTC AAGAGAGTCT GGACTTCACC AGCCAGTTTG GTGCTGCCAT TGTAGGGGCA 
GCTCGCAGGG ACAGAGAACG ATTTCATGAA GCACGACGCA AGAGTGCCTC CTTTTTTCTT 
TCTGCTGAGG AAGAGTTGAG TGGAGCTGGA GGAGCAGGAG GGCGGACTCA GACTTCTCAA 
CAGCAGCTGA GTTCCCAGCC TTCACCACGC CTGCGACCAT CCAAATCTAT AGATGAAGGC 
ATGTTCTCAG GTGATACTTT TATCCATCAC ACACGCAGTA TGCCTCCTGC ATTTGGCTTG 
CCTGAGTACT CCTCACCTGC TCCTCAAGGG GATTACCAGC CTAAGTCTGT ACCTGCTGAC 
TTCTATGGGG CAGGATCCAG ACAACAGCAG CAGCAGCAGC AGCAGCAAGC CCCCACCACA 
ACTTTTATTC ACCCACTAAC TGGAAAAGTA CTGGACCCTT CATCCCCTCT AGGCTTGGCT 
TTGGCTGCAC GGGAACGTGC CTTGAAGGAT GATGGACGTA TACGTAGGGA AAGGACCACT 
GAACATCATT TTACCCGTCA GTTGTCAAGC ATTGGGGCCT TTTCCTCATC CACTTCACAG 
CCAACATCAC ACATTTCCTA CCCAATGGCC TCGTCTTCCT CTGCAACCTC CTCTGCTGCT 
CAATCCACAA ACACCTCTTC ATATCTTGTT ACCTCCTCAT CATTAGCTGC CACCACCACA 
TCCACTCGGC CTCCATCTCC TAGGATCCTG CGTGGAGCAG GGAGTAGCTG GAGTGAAGAA 
GCTGGGGTTG GAGAGCGTGC GGATAGGGAA GCAGCTGGCA CCTCTGCTCC CAGAGAAGGC 
CTAAGGGTTC GTTTCTCCGA GGATAAAACT GTCCACACAC ACCACTATCA ATCTCAGCAA 
TATCAGCCCA CCTTTAAAGA GCGTGAAAGA GAACGTCAAA GAGAGAGGTC AAGAGAAAGA 
GAGAGGGAAA GAGAGAGGGA AAGAGAAATA GAAAAAGCTC AAACCCAGTA TCAGCAACAG 
TCATCACAAC CAATGACCCA GCAGCCTCGA CGCCCCTCAT TCTTGAGGAT GGAGAGTGAA 
GCTGGAGCGT ACATTCTTTC ATTAACGCCT CCATCTCCTC CTCCAACAGC AGCCCCTATG 
CGTCCTACAG CAGGAGCCAC AGGTGAAAGT GGAAGTGGTA CAAGCCTGAT GGTTCTGCCT 
CCCCCAGCTC CCTCAGTAGA TGCAGATGAT GAGTTTGTGT TTGCTGATCC TCTTCCTCCA 
CCTCTAGAGT TTGCCAATAG TTTTGATCGT GGTTTGGGTT ACTCTGCGAT GATTTCTCAC 
AATGTTGCAG CCCGTCAGCA GCGTCATCCT CCACCACCCC CTCCCCCACC ACCCCCGCCC 
ACTTCGAAAG ATTCCTTTAT GACAGGCTTT CCAGCTCACA CACCCCTACC GTCACCTCAG 
GCAGGAGATT CCACAACTTC CAGTCTTACC TCCTATGACA GTGAGGTTGC AAACCTAACA 
CAATCTGCCC CATCGCCCTC CCCATCTTCT CCCAACCCAC TACCACCTCA CTCCCCCTCC 
ACCCTTCAGA CATCTGGACC ACCTCCACCT CCCTCTGTAT CACCTCCTCC ACCACCCAAC 
AGCTACCATA GACCTTTTGG CTTATCTCAG TCACAACATT TATACAACAG TACACACACA 
ACTGGACGCT CCTCCTCTCC CACCCCACCT CTCTCTATTC CAGCTCCACC TGCCTACCGG 
GGGCCACCAA CAGCATCATC CACCCCTGCA ACTTCTGTAT CAATCAGAGG TGCCTCAACT 
GTGACCGCAA GCTGTTCCAC AACTGCTTCT ACAACTGTCA CCTCTCCTAG TACTACTGCT 
GCTGCCACAC CACACACACG CATAGCAACT GTCTCCACAG TAGCTACAGG GGGTAGTAGG 
CGTCCCAGTG CAGAGCATCA TCCACCACCT AGTGCAACCA AGAGTGGTGA GTCCCAGGAG 
ACAGTGGTAG ATTCTGGGAT TGAGGAGCTG GACAGCCGAA GCAGCAGTGA TCATCACTTA 
GACAGCATTC TTGCACTCAG TGGTAGTGGG GTCAGAGAGA GGTTGGAACG AGCAGGGATG 
AGTAGCATAG GTGGAATTGG AGGGAGAGAT GGTGCAACTG AGGTTGACAG GACAAGTGGG 
GCAGACTTTT TAGAGTCTTA CATGAGCTAC CTTGATGGGC AGACTTTTGA AATGCACAAT 
GCCAAAGCCG CTAGCACTGC ATCCACCTAC CCAAAAACAC AGAGGTACAG AGAAGGCACC 
CGGGCTGGAG ATCTTCGACG CCAGACCAGC ACAGCACCTC CTGCATACCA CCGTCGTAGA 
GAGGAGGAAG AGGAAGAGGA TGAGGCCGAG GAGGTAGAGG AGGAGGATTT GAGTGCCCGT 
GATGGCTATG GGATGAGGGA AGGGCCAGGG ATTGCTCGCT CCTCTGTAAT GTACTTTGAC 
CGGCCCCGCA CTCCAGAGAT AAAACCACTG TGGGGAGATG GGTCGACTGG AGCATCACAG 
ACCATGGGGG AGGGAGGGGG CCAGATAGTG GGAACATATG TGGAGCCACG TAAACTACAT 
CCTCCCATGA CTGGTATGAA AGCAAACATC ATTAATGAAC TAAGCACCAA ATTACAGCAG 
AGAAGTAAAG GAACAGAGAA CTGGGGGGCC CAGAGATCAT TGAGCAGACA TAGACACAGG 
TTCTCAGAGG ACTCAACAGG TGCACTGAGT CCTCCCTCTG TCCGCTCTAG CTCCCCATCT 
CCAGTTCAGC TCTCTCAGCC TGCCTTGTCT CCCTCTCCCA CTCCTTCCTC CCAGCCACTT 
TACTCAAACT GGGCTCGATC TCCTTCCCCT CAGCCCCCTG CTGCCTCATC CCAGCCCCCT 
CCCCGATCCC ATTCACCTGT TTCCCCTTCA TCATATTCAC CATACCCCAC CTCACCCAAA 
CACCGGCCTG TTTATCGCAC CAAAGCTCTG GAGTTCCAAT TTATTCCCAG TCGAGAGTCA 
CGAAAGTCAG AGTCGCATCA TGCCCAGCGC AGACGAGCAC CCAGCCCTTT AATTTCTCCA 
TCTGACCGTC CCAAGCTTGG CCCTCCCCGC CCCTCTTCCC TTCCTATTCT CCCCACTACA 
CCCCTCTATA GCAGCCTGTA TGACCTTCGT GGTTCTATTA CTCCACCCTC ACCTGCCAAT 
CCCCTTGGGG ACCCCTACTT TTCCCCCTCC CCCCCTCTAT TTGCCCCCTC GGGTGCTCCC 
CCTCCTCCAA ATTCCTCTCT GGTGGTCTCA CGGTCCCTGT CACCCACTCA CTTCCTGTCA 
GGGACTTCAT CCCCACCTCT CCACCCCATT CCTCCACCCA CTTGCCTGAG TTACCCCCAT 
CTTCCACCCC CCTCACCCAC AAAGCCCTTT GCCTCAAAAC CTCTCCCATA CTGGACCAAG 
TATGATGTTG CAGATTGGCT GGGCTACCTG AACCTGGGTG AACACAGGGA GCGATTCTTA 
GACAATGAAA TTGATGGCAC GCACCTGCCC TCTCTTACCA AGGATGACTA CCTGGACCTG 
GGTGTGACCC GAGTTGGCCA TCGCATGAAC ATAGAGAGGG CGCTCAGGAG AGTTATGGAC 
AGGCTGTCTT CTAGCACTTT CCCCATTTCT GCTCTCTCTC CTCGAAATGG ACGGACAAAC 
AGAAGGAGAG ATGAGGCCAA CCGGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329733.1
CDS	286..7695
Translation	MTMPLSPLSSDEEQQRMLGNSQHLYPGAEEEEEEEEEMDDDGMDEEGEEENGELEMDDEEEVDDEDDIRRGPGHLRGGREEGHRQKGTMAGRLPGDRPIRPGNPGHAANPYSSNPGPTHQTSTNQQQQQPQRKLRERSSSNAPDDTHVALMVFRIGIPDIKQTKCLRFNPDATVWKAKQQVMCSLTESLRDVLNYGLFQPATDGHDAKFLEEERPLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKASLKKFMDYIQTSAVEKVAKLIDKGLDPNYHDPDTGETPLTLAVQTEPGGSEIIRVLVMGGAHIDFRARDGLTPLHKAVRAHAHTALLTLLSLGASSDYKDSRGLTPLYHTVLIGGDTSCCETLLYHRARLGLRDENGWDETHQACQNGNSQHLEHLLFYGADSSSQNASGNTALHICALYNKESCARILLYRGAKKDTKNNSGQNSFQVAVMSGHFELGEIIKNHRESDVVPFLESPKYAPQRRESSRTLGLPHPHPFLRANSDNSMNVPDWIAFPNAAGSSIVSVQGLKHGGTLRSSSSPRGARTRSPSRGRTGDRDDRSRQTRGRQGAGSISSQGTASGQRRRLYSAVPGRVFVATRSHSAQGDREISFNKGDKVKVLSVGEGGYWEGTVRGRTGWFPSDCVEEVALRSLESRSESRSERAKRLFRHYTVGSYDSFDAPSDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMVDRASAPWKKKSEFESSQATEKKRTVYQMALNKLDEILAAAQQTINTNEAPGPRVQGVKRERGRGFYNEPNFDQTGMGMTSSGAGLGYDRGQFVSGHGPPHGMLRQKSIGVPEEEKQFLHPPAMKFARSLSVPGPDEIPPPPTTAPPEPPFSSAPPLGFRGKTSQQPSVSMSQSTSSAAHYQLYSQSVHVTHGPRDRSPGPPTGSGTGDLAYSHAQAHPSVPSRTASRKVIGYTGDLAGAGGVAGAKAAALRRGYSNAVPPTSVATVIPQQQQTTQNQPSRMDRSGMGVGAGGVPKGGARRGKGPLVKQSKVEDLRQGQDTLEAKVSVEKSSIPIPTIIVKAPSTSSSGRSSQGSSVEAEPPPPGESDSSKPEPVVPPPAPTVPPPAPPSIPAPPPPSLPSLRTQESLDFTSQFGAAIVGAARRDRERFHEARRKSASFFLSAEEELSGAGGAGGRTQTSQQQLSSQPSPRLRPSKSIDEGMFSGDTFIHHTRSMPPAFGLPEYSSPAPQGDYQPKSVPADFYGAGSRQQQQQQQQQAPTTTFIHPLTGKVLDPSSPLGLALAARERALKDDGRIRRERTTEHHFTRQLSSIGAFSSSTSQPTSHISYPMASSSSATSSAAQSTNTSSYLVTSSSLAATTTSTRPPSPRILRGAGSSWSEEAGVGERADREAAGTSAPREGLRVRFSEDKTVHTHHYQSQQYQPTFKERERERQRERSREREREREREREIEKAQTQYQQQSSQPMTQQPRRPSFLRMESEAGAYILSLTPPSPPPTAAPMRPTAGATGESGSGTSLMVLPPPAPSVDADDEFVFADPLPPPLEFANSFDRGLGYSAMISHNVAARQQRHPPPPPPPPPPPTSKDSFMTGFPAHTPLPSPQAGDSTTSSLTSYDSEVANLTQSAPSPSPSSPNPLPPHSPSTLQTSGPPPPPSVSPPPPPNSYHRPFGLSQSQHLYNSTHTTGRSSSPTPPLSIPAPPAYRGPPTASSTPATSVSIRGASTVTASCSTTASTTVTSPSTTAAATPHTRIATVSTVATGGSRRPSAEHHPPPSATKSGESQETVVDSGIEELDSRSSSDHHLDSILALSGSGVRERLERAGMSSIGGIGGRDGATEVDRTSGADFLESYMSYLDGQTFEMHNAKAASTASTYPKTQRYREGTRAGDLRRQTSTAPPAYHRRREEEEEEDEAEEVEEEDLSARDGYGMREGPGIARSSVMYFDRPRTPEIKPLWGDGSTGASQTMGEGGGQIVGTYVEPRKLHPPMTGMKANIINELSTKLQQRSKGTENWGAQRSLSRHRHRFSEDSTGALSPPSVRSSSPSPVQLSQPALSPSPTPSSQPLYSNWARSPSPQPPAASSQPPPRSHSPVSPSSYSPYPTSPKHRPVYRTKALEFQFIPSRESRKSESHHAQRRRAPSPLISPSDRPKLGPPRPSSLPILPTTPLYSSLYDLRGSITPPSPANPLGDPYFSPSPPLFAPSGAPPPPNSSLVVSRSLSPTHFLSGTSSPPLHPIPPPTCLSYPHLPPPSPTKPFASKPLPYWTKYDVADWLGYLNLGEHRERFLDNEIDGTHLPSLTKDDYLDLGVTRVGHRMNIERALRRVMDRLSSSTFPISALSPRNGRTNRRRDEANRS

Target ORF information:

RefSeq Version	XM_021474058.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474058.1

ORF Insert Sequence:

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGTG 
GACAGAGCCT CTGCCCCATG GAAAAAAAAA TCAGAGTTCG AATCTTCACA AGCTACAGAG 
AAGAAGAGGA CAGTTTATCA GATGGCTTTG AATAAACTGG ATGAGATTTT AGCAGCAGCA 
CAGCAGACCA TTAATACAAA TGAGGCTCCA GGGCCCCGGG TTCAGGGGGT AAAAAGAGAA 
CGGGGCAGAG GCTTTTATAA TGAGCCAAAT TTCGACCAAA CTGGAATGGG GATGACATCA 
TCTGGGGCAG GGTTAGGCTA CGACCGTGGT CAATTTGTGT CTGGCCATGG CCCCCCGCAC 
GGTATGCTGC GACAGAAGTC TATCGGGGTT CCTGAGGAGG AGAAGCAGTT TCTTCATCCT 
CCAGCCATGA AGTTTGCCCG TAGTCTTTCA GTACCTGGCC CAGATGAAAT CCCTCCTCCA 
CCTACTACAG CACCCCCTGA GCCCCCTTTT TCCTCAGCTC CTCCGCTTGG CTTTAGGGGC 
AAGACATCTC AACAGCCGTC TGTTTCTATG TCTCAGTCTA CTTCCTCTGC TGCACATTAT 
CAACTCTACT CACAGTCAGT ACATGTTACT CACGGACCAA GGGACAGGTC CCCTGGACCA 
CCTACTGGGT CTGGAACTGG GGACCTTGCT TATTCCCATG CCCAAGCCCA CCCCTCTGTA 
CCAAGTAGGA CTGCCTCCAG GAAGGTGATT GGGTACACAG GAGATCTTGC AGGGGCTGGA 
GGAGTGGCTG GGGCCAAAGC TGCAGCATTG AGAAGGGGTT ACAGCAATGC AGTCCCACCC 
ACCAGTGTCG CTACTGTGAT TCCACAACAG CAGCAGACTA CTCAGAACCA ACCTTCCAGA 
ATGGACCGGA GTGGAATGGG GGTAGGGGCT GGTGGTGTAC CAAAAGGCGG TGCTCGTAGA 
GGAAAGGGGC CCTTGGTGAA ACAGTCAAAG GTGGAAGACC TGCGTCAAGG TCAAGACACT 
CTAGAAGCAA AGGTGTCAGT GGAGAAGAGC TCTATTCCAA TCCCAACTAT CATTGTTAAA 
GCCCCTTCCA CCAGCAGTAG TGGACGCAGT AGCCAAGGCA GCAGTGTGGA GGCTGAACCA 
CCGCCCCCAG GAGAAAGTGA CAGTTCTAAA CCAGAACCTG TTGTTCCCCC ACCTGCACCA 
ACCGTGCCAC CACCAGCCCC TCCATCTATA CCTGCTCCTC CACCCCCATC ACTGCCATCA 
CTTCGTACTC AAGAGAGTCT GGACTTCACC AGCCAGTTTG GTGCTGCCAT TGTAGGGGCA 
GCTCGCAGGG ACAGAGAACG ATTTCATGAA GCACGACGCA AGAGTGCCTC CTTTTTTCTT 
TCTGCTGAGG AAGAGTTGAG TGGAGCTGGA GGAGCAGGAG GGCGGACTCA GACTTCTCAA 
CAGCAGCTGA GTTCCCAGCC TTCACCACGC CTGCGACCAT CCAAATCTAT AGATGAAGGC 
ATGTTCTCAG GTGATACTTT TATCCATCAC ACACGCAGTA TGCCTCCTGC ATTTGGCTTG 
CCTGAGTACT CCTCACCTGC TCCTCAAGGG GATTACCAGC CTAAGTCTGT ACCTGCTGAC 
TTCTATGGGG CAGGATCCAG ACAACAGCAG CAGCAGCAGC AGCAGCAAGC CCCCACCACA 
ACTTTTATTC ACCCACTAAC TGGAAAAGTA CTGGACCCTT CATCCCCTCT AGGCTTGGCT 
TTGGCTGCAC GGGAACGTGC CTTGAAGGAT GATGGACGTA TACGTAGGGA AAGGACCACT 
GAACATCATT TTACCCGTCA GTTGTCAAGC ATTGGGGCCT TTTCCTCATC CACTTCACAG 
CCAACATCAC ACATTTCCTA CCCAATGGCC TCGTCTTCCT CTGCAACCTC CTCTGCTGCT 
CAATCCACAA ACACCTCTTC ATATCTTGTT ACCTCCTCAT CATTAGCTGC CACCACCACA 
TCCACTCGGC CTCCATCTCC TAGGATCCTG CGTGGAGCAG GGAGTAGCTG GAGTGAAGAA 
GCTGGGGTTG GAGAGCGTGC GGATAGGGAA GCAGCTGGCA CCTCTGCTCC CAGAGAAGGC 
CTAAGGGTTC GTTTCTCCGA GGATAAAACT GTCCACACAC ACCACTATCA ATCTCAGCAA 
TATCAGCCCA CCTTTAAAGA GCGTGAAAGA GAACGTCAAA GAGAGAGGTC AAGAGAAAGA 
GAGAGGGAAA GAGAGAGGGA AAGAGAAATA GAAAAAGCTC AAACCCAGTA TCAGCAACAG 
TCATCACAAC CAATGACCCA GCAGCCTCGA CGCCCCTCAT TCTTGAGGAT GGAGAGTGAA 
GCTGGAGCGT ACATTCTTTC ATTAACGCCT CCATCTCCTC CTCCAACAGC AGCCCCTATG 
CGTCCTACAG CAGGAGCCAC AGGTGAAAGT GGAAGTGGTA CAAGCCTGAT GGTTCTGCCT 
CCCCCAGCTC CCTCAGTAGA TGCAGATGAT GAGTTTGTGT TTGCTGATCC TCTTCCTCCA 
CCTCTAGAGT TTGCCAATAG TTTTGATCGT GGTTTGGGTT ACTCTGCGAT GATTTCTCAC 
AATGTTGCAG CCCGTCAGCA GCGTCATCCT CCACCACCCC CTCCCCCACC ACCCCCGCCC 
ACTTCGAAAG ATTCCTTTAT GACAGGCTTT CCAGCTCACA CACCCCTACC GTCACCTCAG 
GCAGGAGATT CCACAACTTC CAGTCTTACC TCCTATGACA GTGAGGTTGC AAACCTAACA 
CAATCTGCCC CATCGCCCTC CCCATCTTCT CCCAACCCAC TACCACCTCA CTCCCCCTCC 
ACCCTTCAGA CATCTGGACC ACCTCCACCT CCCTCTGTAT CACCTCCTCC ACCACCCAAC 
AGCTACCATA GACCTTTTGG CTTATCTCAG TCACAACATT TATACAACAG TACACACACA 
ACTGGACGCT CCTCCTCTCC CACCCCACCT CTCTCTATTC CAGCTCCACC TGCCTACCGG 
GGGCCACCAA CAGCATCATC CACCCCTGCA ACTTCTGTAT CAATCAGAGG TGCCTCAACT 
GTGACCGCAA GCTGTTCCAC AACTGCTTCT ACAACTGTCA CCTCTCCTAG TACTACTGCT 
GCTGCCACAC CACACACACG CATAGCAACT GTCTCCACAG TAGCTACAGG GGGTAGTAGG 
CGTCCCAGTG CAGAGCATCA TCCACCACCT AGTGCAACCA AGAGTGGTGA GTCCCAGGAG 
ACAGTGGTAG ATTCTGGGAT TGAGGAGCTG GACAGCCGAA GCAGCAGTGA TCATCACTTA 
GACAGCATTC TTGCACTCAG TGGTAGTGGG GTCAGAGAGA GGTTGGAACG AGCAGGGATG 
AGTAGCATAG GTGGAATTGG AGGGAGAGAT GGTGCAACTG AGGTTGACAG GACAAGTGGG 
GCAGACTTTT TAGAGTCTTA CATGAGCTAC CTTGATGGGC AGACTTTTGA AATGCACAAT 
GCCAAAGCCG CTAGCACTGC ATCCACCTAC CCAAAAACAC AGAGGTACAG AGAAGGCACC 
CGGGCTGGAG ATCTTCGACG CCAGACCAGC ACAGCACCTC CTGCATACCA CCGTCGTAGA 
GAGGAGGAAG AGGAAGAGGA TGAGGCCGAG GAGGTAGAGG AGGAGGATTT GAGTGCCCGT 
GATGGCTATG GGATGAGGGA AGGGCCAGGG ATTGCTCGCT CCTCTGTAAT GTACTTTGAC 
CGGCCCCGCA CTCCAGAGAT AAAACCACTG TGGGGAGATG GGTCGACTGG AGCATCACAG 
ACCATGGGGG AGGGAGGGGG CCAGATAGTG GGAACATATG TGGAGCCACG TAAACTACAT 
CCTCCCATGA CTGGTATGAA AGCAAACATC ATTAATGAAC TAAGCACCAA ATTACAGCAG 
AGAAGTAAAG GAACAGAGAA CTGGGGGGCC CAGAGATCAT TGAGCAGACA TAGACACAGG 
TTCTCAGAGG ACTCAACAGG TGCACTGAGT CCTCCCTCTG TCCGCTCTAG CTCCCCATCT 
CCAGTTCAGC TCTCTCAGCC TGCCTTGTCT CCCTCTCCCA CTCCTTCCTC CCAGCCACTT 
TACTCAAACT GGGCTCGATC TCCTTCCCCT CAGCCCCCTG CTGCCTCATC CCAGCCCCCT 
CCCCGATCCC ATTCACCTGT TTCCCCTTCA TCATATTCAC CATACCCCAC CTCACCCAAA 
CACCGGCCTG TTTATCGCAC CAAAGCTCTG GAGTTCCAAT TTATTCCCAG TCGAGAGTCA 
CGAAAGTCAG AGTCGCATCA TGCCCAGCGC AGACGAGCAC CCAGCCCTTT AATTTCTCCA 
TCTGACCGTC CCAAGCTTGG CCCTCCCCGC CCCTCTTCCC TTCCTATTCT CCCCACTACA 
CCCCTCTATA GCAGCCTGTA TGACCTTCGT GGTTCTATTA CTCCACCCTC ACCTGCCAAT 
CCCCTTGGGG ACCCCTACTT TTCCCCCTCC CCCCCTCTAT TTGCCCCCTC GGGTGCTCCC 
CCTCCTCCAA ATTCCTCTCT GGTGGTCTCA CGGTCCCTGT CACCCACTCA CTTCCTGTCA 
GGGACTTCAT CCCCACCTCT CCACCCCATT CCTCCACCCA CTTGCCTGAG TTACCCCCAT 
CTTCCACCCC CCTCACCCAC AAAGCCCTTT GCCTCAAAAC CTCTCCCATA CTGGACCAAG 
TATGATGTTG CAGATTGGCT GGGCTACCTG AACCTGGGTG AACACAGGGA GCGATTCTTA 
GACAATGAAA TTGATGGCAC GCACCTGCCC TCTCTTACCA AGGATGACTA CCTGGACCTG 
GGTGTGACCC GAGTTGGCCA TCGCATGAAC ATAGAGAGGG CGCTCAGGAG AGTTATGGAC 
AGGCTGTCTT CTAGCACTTT CCCCATTTCT GCTCTCTCTC CTCGAAATGG ACGGACAAAC 
AGAAGGAGAG ATGAGGCCAA CCGGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_021471432.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327107.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X2, mRNA.

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CloneID	ODa141764
Clone ID Related Accession (Same CDS sequence)	XM_021471431.1 , XM_021474051.1 , XM_021474058.1 , XM_021471432.1
Accession Version	XM_021471432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394533.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGTG 
GACAGAGCCT CTGCCCCATG GAAAAAAAAA TCAGAGTTCG AATCTTCACA AGCTACAGAG 
AAGAAGAGGA CAGTTTATCA GATGGCTTTG AATAAACTGG ATGAGATTTT AGCAGCAGCA 
CAGCAGACCA TTAATACAAA TGAGGCTCCA GGGCCCCGGG TTCAGGGGGT AAAAAGAGAA 
CGGGGCAGAG GCTTTTATAA TGAGCCAAAT TTCGACCAAA CTGGAATGGG GATGACATCA 
TCTGGGGCAG GGTTAGGCTA CGACCGTGGT CAATTTGTGT CTGGCCATGG CCCCCCGCAC 
GGTATGCTGC GACAGAAGTC TATCGGGGTT CCTGAGGAGG AGAAGCAGTT TCTTCATCCT 
CCAGCCATGA AGTTTGCCCG TAGTCTTTCA GTACCTGGCC CAGATGAAAT CCCTCCTCCA 
CCTACTACAG CACCCCCTGA GCCCCCTTTT TCCTCAGCTC CTCCGCTTGG CTTTAGGGGC 
AAGACATCTC AACAGCCGTC TGTTTCTATG TCTCAGTCTA CTTCCTCTGC TGCACATTAT 
CAACTCTACT CACAGTCAGT ACATGTTACT CACGGACCAA GGGACAGGTC CCCTGGACCA 
CCTACTGGGT CTGGAACTGG GGACCTTGCT TATTCCCATG CCCAAGCCCA CCCCTCTGTA 
CCAAGTAGGA CTGCCTCCAG GAAGGTGATT GGGTACACAG GAGATCTTGC AGGGGCTGGA 
GGAGTGGCTG GGGCCAAAGC TGCAGCATTG AGAAGGGGTT ACAGCAATGC AGTCCCACCC 
ACCAGTGTCG CTACTGTGAT TCCACAACAG CAGCAGACTA CTCAGAACCA ACCTTCCAGA 
ATGGACCGGA GTGGAATGGG GGTAGGGGCT GGTGGTGTAC CAAAAGGCGG TGCTCGTAGA 
GGAAAGGGGC CCTTGGTGAA ACAGTCAAAG GTGGAAGACC TGCGTCAAGG TCAAGACACT 
CTAGAAGCAA AGGTGTCAGT GGAGAAGAGC TCTATTCCAA TCCCAACTAT CATTGTTAAA 
GCCCCTTCCA CCAGCAGTAG TGGACGCAGT AGCCAAGGCA GCAGTGTGGA GGCTGAACCA 
CCGCCCCCAG GAGAAAGTGA CAGTTCTAAA CCAGAACCTG TTGTTCCCCC ACCTGCACCA 
ACCGTGCCAC CACCAGCCCC TCCATCTATA CCTGCTCCTC CACCCCCATC ACTGCCATCA 
CTTCGTACTC AAGAGAGTCT GGACTTCACC AGCCAGTTTG GTGCTGCCAT TGTAGGGGCA 
GCTCGCAGGG ACAGAGAACG ATTTCATGAA GCACGACGCA AGAGTGCCTC CTTTTTTCTT 
TCTGCTGAGG AAGAGTTGAG TGGAGCTGGA GGAGCAGGAG GGCGGACTCA GACTTCTCAA 
CAGCAGCTGA GTTCCCAGCC TTCACCACGC CTGCGACCAT CCAAATCTAT AGATGAAGGC 
ATGTTCTCAG GTGATACTTT TATCCATCAC ACACGCAGTA TGCCTCCTGC ATTTGGCTTG 
CCTGAGTACT CCTCACCTGC TCCTCAAGGG GATTACCAGC CTAAGTCTGT ACCTGCTGAC 
TTCTATGGGG CAGGATCCAG ACAACAGCAG CAGCAGCAGC AGCAGCAAGC CCCCACCACA 
ACTTTTATTC ACCCACTAAC TGGAAAAGTA CTGGACCCTT CATCCCCTCT AGGCTTGGCT 
TTGGCTGCAC GGGAACGTGC CTTGAAGGAT GATGGACGTA TACGTAGGGA AAGGACCACT 
GAACATCATT TTACCCGTCA GTTGTCAAGC ATTGGGGCCT TTTCCTCATC CACTTCACAG 
CCAACATCAC ACATTTCCTA CCCAATGGCC TCGTCTTCCT CTGCAACCTC CTCTGCTGCT 
CAATCCACAA ACACCTCTTC ATATCTTGTT ACCTCCTCAT CATTAGCTGC CACCACCACA 
TCCACTCGGC CTCCATCTCC TAGGATCCTG CGTGGAGCAG GGAGTAGCTG GAGTGAAGAA 
GCTGGGGTTG GAGAGCGTGC GGATAGGGAA GCAGCTGGCA CCTCTGCTCC CAGAGAAGGC 
CTAAGGGTTC GTTTCTCCGA GGATAAAACT GTCCACACAC ACCACTATCA ATCTCAGCAA 
TATCAGCCCA CCTTTAAAGA GCGTGAAAGA GAACGTCAAA GAGAGAGGTC AAGAGAAAGA 
GAGAGGGAAA GAGAGAGGGA AAGAGAAATA GAAAAAGCTC AAACCCAGTA TCAGCAACAG 
TCATCACAAC CAATGACCCA GCAGCCTCGA CGCCCCTCAT TCTTGAGGAT GGAGAGTGAA 
GCTGGAGCGT ACATTCTTTC ATTAACGCCT CCATCTCCTC CTCCAACAGC AGCCCCTATG 
CGTCCTACAG CAGGAGCCAC AGGTGAAAGT GGAAGTGGTA CAAGCCTGAT GGTTCTGCCT 
CCCCCAGCTC CCTCAGTAGA TGCAGATGAT GAGTTTGTGT TTGCTGATCC TCTTCCTCCA 
CCTCTAGAGT TTGCCAATAG TTTTGATCGT GGTTTGGGTT ACTCTGCGAT GATTTCTCAC 
AATGTTGCAG CCCGTCAGCA GCGTCATCCT CCACCACCCC CTCCCCCACC ACCCCCGCCC 
ACTTCGAAAG ATTCCTTTAT GACAGGCTTT CCAGCTCACA CACCCCTACC GTCACCTCAG 
GCAGGAGATT CCACAACTTC CAGTCTTACC TCCTATGACA GTGAGGTTGC AAACCTAACA 
CAATCTGCCC CATCGCCCTC CCCATCTTCT CCCAACCCAC TACCACCTCA CTCCCCCTCC 
ACCCTTCAGA CATCTGGACC ACCTCCACCT CCCTCTGTAT CACCTCCTCC ACCACCCAAC 
AGCTACCATA GACCTTTTGG CTTATCTCAG TCACAACATT TATACAACAG TACACACACA 
ACTGGACGCT CCTCCTCTCC CACCCCACCT CTCTCTATTC CAGCTCCACC TGCCTACCGG 
GGGCCACCAA CAGCATCATC CACCCCTGCA ACTTCTGTAT CAATCAGAGG TGCCTCAACT 
GTGACCGCAA GCTGTTCCAC AACTGCTTCT ACAACTGTCA CCTCTCCTAG TACTACTGCT 
GCTGCCACAC CACACACACG CATAGCAACT GTCTCCACAG TAGCTACAGG GGGTAGTAGG 
CGTCCCAGTG CAGAGCATCA TCCACCACCT AGTGCAACCA AGAGTGGTGA GTCCCAGGAG 
ACAGTGGTAG ATTCTGGGAT TGAGGAGCTG GACAGCCGAA GCAGCAGTGA TCATCACTTA 
GACAGCATTC TTGCACTCAG TGGTAGTGGG GTCAGAGAGA GGTTGGAACG AGCAGGGATG 
AGTAGCATAG GTGGAATTGG AGGGAGAGAT GGTGCAACTG AGGTTGACAG GACAAGTGGG 
GCAGACTTTT TAGAGTCTTA CATGAGCTAC CTTGATGGGC AGACTTTTGA AATGCACAAT 
GCCAAAGCCG CTAGCACTGC ATCCACCTAC CCAAAAACAC AGAGGTACAG AGAAGGCACC 
CGGGCTGGAG ATCTTCGACG CCAGACCAGC ACAGCACCTC CTGCATACCA CCGTCGTAGA 
GAGGAGGAAG AGGAAGAGGA TGAGGCCGAG GAGGTAGAGG AGGAGGATTT GAGTGCCCGT 
GATGGCTATG GGATGAGGGA AGGGCCAGGG ATTGCTCGCT CCTCTGTAAT GTACTTTGAC 
CGGCCCCGCA CTCCAGAGAT AAAACCACTG TGGGGAGATG GGTCGACTGG AGCATCACAG 
ACCATGGGGG AGGGAGGGGG CCAGATAGTG GGAACATATG TGGAGCCACG TAAACTACAT 
CCTCCCATGA CTGGTATGAA AGCAAACATC ATTAATGAAC TAAGCACCAA ATTACAGCAG 
AGAAGTAAAG GAACAGAGAA CTGGGGGGCC CAGAGATCAT TGAGCAGACA TAGACACAGG 
TTCTCAGAGG ACTCAACAGG TGCACTGAGT CCTCCCTCTG TCCGCTCTAG CTCCCCATCT 
CCAGTTCAGC TCTCTCAGCC TGCCTTGTCT CCCTCTCCCA CTCCTTCCTC CCAGCCACTT 
TACTCAAACT GGGCTCGATC TCCTTCCCCT CAGCCCCCTG CTGCCTCATC CCAGCCCCCT 
CCCCGATCCC ATTCACCTGT TTCCCCTTCA TCATATTCAC CATACCCCAC CTCACCCAAA 
CACCGGCCTG TTTATCGCAC CAAAGCTCTG GAGTTCCAAT TTATTCCCAG TCGAGAGTCA 
CGAAAGTCAG AGTCGCATCA TGCCCAGCGC AGACGAGCAC CCAGCCCTTT AATTTCTCCA 
TCTGACCGTC CCAAGCTTGG CCCTCCCCGC CCCTCTTCCC TTCCTATTCT CCCCACTACA 
CCCCTCTATA GCAGCCTGTA TGACCTTCGT GGTTCTATTA CTCCACCCTC ACCTGCCAAT 
CCCCTTGGGG ACCCCTACTT TTCCCCCTCC CCCCCTCTAT TTGCCCCCTC GGGTGCTCCC 
CCTCCTCCAA ATTCCTCTCT GGTGGTCTCA CGGTCCCTGT CACCCACTCA CTTCCTGTCA 
GGGACTTCAT CCCCACCTCT CCACCCCATT CCTCCACCCA CTTGCCTGAG TTACCCCCAT 
CTTCCACCCC CCTCACCCAC AAAGCCCTTT GCCTCAAAAC CTCTCCCATA CTGGACCAAG 
TATGATGTTG CAGATTGGCT GGGCTACCTG AACCTGGGTG AACACAGGGA GCGATTCTTA 
GACAATGAAA TTGATGGCAC GCACCTGCCC TCTCTTACCA AGGATGACTA CCTGGACCTG 
GGTGTGACCC GAGTTGGCCA TCGCATGAAC ATAGAGAGGG CGCTCAGGAG AGTTATGGAC 
AGGCTGTCTT CTAGCACTTT CCCCATTTCT GCTCTCTCTC CTCGAAATGG ACGGACAAAC 
AGAAGGAGAG ATGAGGCCAA CCGGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327107.1
CDS	286..7695
Translation	MTMPLSPLSSDEEQQRMLGNSQHLYPGAEEEEEEEEEMDDDGMDEEGEEENGELEMDDEEEVDDEDDIRRGPGHLRGGREEGHRQKGTMAGRLPGDRPIRPGNPGHAANPYSSNPGPTHQTSTNQQQQQPQRKLRERSSSNAPDDTHVALMVFRIGIPDIKQTKCLRFNPDATVWKAKQQVMCSLTESLRDVLNYGLFQPATDGHDAKFLEEERPLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKASLKKFMDYIQTSAVEKVAKLIDKGLDPNYHDPDTGETPLTLAVQTEPGGSEIIRVLVMGGAHIDFRARDGLTPLHKAVRAHAHTALLTLLSLGASSDYKDSRGLTPLYHTVLIGGDTSCCETLLYHRARLGLRDENGWDETHQACQNGNSQHLEHLLFYGADSSSQNASGNTALHICALYNKESCARILLYRGAKKDTKNNSGQNSFQVAVMSGHFELGEIIKNHRESDVVPFLESPKYAPQRRESSRTLGLPHPHPFLRANSDNSMNVPDWIAFPNAAGSSIVSVQGLKHGGTLRSSSSPRGARTRSPSRGRTGDRDDRSRQTRGRQGAGSISSQGTASGQRRRLYSAVPGRVFVATRSHSAQGDREISFNKGDKVKVLSVGEGGYWEGTVRGRTGWFPSDCVEEVALRSLESRSESRSERAKRLFRHYTVGSYDSFDAPSDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMVDRASAPWKKKSEFESSQATEKKRTVYQMALNKLDEILAAAQQTINTNEAPGPRVQGVKRERGRGFYNEPNFDQTGMGMTSSGAGLGYDRGQFVSGHGPPHGMLRQKSIGVPEEEKQFLHPPAMKFARSLSVPGPDEIPPPPTTAPPEPPFSSAPPLGFRGKTSQQPSVSMSQSTSSAAHYQLYSQSVHVTHGPRDRSPGPPTGSGTGDLAYSHAQAHPSVPSRTASRKVIGYTGDLAGAGGVAGAKAAALRRGYSNAVPPTSVATVIPQQQQTTQNQPSRMDRSGMGVGAGGVPKGGARRGKGPLVKQSKVEDLRQGQDTLEAKVSVEKSSIPIPTIIVKAPSTSSSGRSSQGSSVEAEPPPPGESDSSKPEPVVPPPAPTVPPPAPPSIPAPPPPSLPSLRTQESLDFTSQFGAAIVGAARRDRERFHEARRKSASFFLSAEEELSGAGGAGGRTQTSQQQLSSQPSPRLRPSKSIDEGMFSGDTFIHHTRSMPPAFGLPEYSSPAPQGDYQPKSVPADFYGAGSRQQQQQQQQQAPTTTFIHPLTGKVLDPSSPLGLALAARERALKDDGRIRRERTTEHHFTRQLSSIGAFSSSTSQPTSHISYPMASSSSATSSAAQSTNTSSYLVTSSSLAATTTSTRPPSPRILRGAGSSWSEEAGVGERADREAAGTSAPREGLRVRFSEDKTVHTHHYQSQQYQPTFKERERERQRERSREREREREREREIEKAQTQYQQQSSQPMTQQPRRPSFLRMESEAGAYILSLTPPSPPPTAAPMRPTAGATGESGSGTSLMVLPPPAPSVDADDEFVFADPLPPPLEFANSFDRGLGYSAMISHNVAARQQRHPPPPPPPPPPPTSKDSFMTGFPAHTPLPSPQAGDSTTSSLTSYDSEVANLTQSAPSPSPSSPNPLPPHSPSTLQTSGPPPPPSVSPPPPPNSYHRPFGLSQSQHLYNSTHTTGRSSSPTPPLSIPAPPAYRGPPTASSTPATSVSIRGASTVTASCSTTASTTVTSPSTTAAATPHTRIATVSTVATGGSRRPSAEHHPPPSATKSGESQETVVDSGIEELDSRSSSDHHLDSILALSGSGVRERLERAGMSSIGGIGGRDGATEVDRTSGADFLESYMSYLDGQTFEMHNAKAASTASTYPKTQRYREGTRAGDLRRQTSTAPPAYHRRREEEEEEDEAEEVEEEDLSARDGYGMREGPGIARSSVMYFDRPRTPEIKPLWGDGSTGASQTMGEGGGQIVGTYVEPRKLHPPMTGMKANIINELSTKLQQRSKGTENWGAQRSLSRHRHRFSEDSTGALSPPSVRSSSPSPVQLSQPALSPSPTPSSQPLYSNWARSPSPQPPAASSQPPPRSHSPVSPSSYSPYPTSPKHRPVYRTKALEFQFIPSRESRKSESHHAQRRRAPSPLISPSDRPKLGPPRPSSLPILPTTPLYSSLYDLRGSITPPSPANPLGDPYFSPSPPLFAPSGAPPPPNSSLVVSRSLSPTHFLSGTSSPPLHPIPPPTCLSYPHLPPPSPTKPFASKPLPYWTKYDVADWLGYLNLGEHRERFLDNEIDGTHLPSLTKDDYLDLGVTRVGHRMNIERALRRVMDRLSSSTFPISALSPRNGRTNRRRDEANRS

Target ORF information:

RefSeq Version	XM_021471432.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471432.1

ORF Insert Sequence:

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGTG 
GACAGAGCCT CTGCCCCATG GAAAAAAAAA TCAGAGTTCG AATCTTCACA AGCTACAGAG 
AAGAAGAGGA CAGTTTATCA GATGGCTTTG AATAAACTGG ATGAGATTTT AGCAGCAGCA 
CAGCAGACCA TTAATACAAA TGAGGCTCCA GGGCCCCGGG TTCAGGGGGT AAAAAGAGAA 
CGGGGCAGAG GCTTTTATAA TGAGCCAAAT TTCGACCAAA CTGGAATGGG GATGACATCA 
TCTGGGGCAG GGTTAGGCTA CGACCGTGGT CAATTTGTGT CTGGCCATGG CCCCCCGCAC 
GGTATGCTGC GACAGAAGTC TATCGGGGTT CCTGAGGAGG AGAAGCAGTT TCTTCATCCT 
CCAGCCATGA AGTTTGCCCG TAGTCTTTCA GTACCTGGCC CAGATGAAAT CCCTCCTCCA 
CCTACTACAG CACCCCCTGA GCCCCCTTTT TCCTCAGCTC CTCCGCTTGG CTTTAGGGGC 
AAGACATCTC AACAGCCGTC TGTTTCTATG TCTCAGTCTA CTTCCTCTGC TGCACATTAT 
CAACTCTACT CACAGTCAGT ACATGTTACT CACGGACCAA GGGACAGGTC CCCTGGACCA 
CCTACTGGGT CTGGAACTGG GGACCTTGCT TATTCCCATG CCCAAGCCCA CCCCTCTGTA 
CCAAGTAGGA CTGCCTCCAG GAAGGTGATT GGGTACACAG GAGATCTTGC AGGGGCTGGA 
GGAGTGGCTG GGGCCAAAGC TGCAGCATTG AGAAGGGGTT ACAGCAATGC AGTCCCACCC 
ACCAGTGTCG CTACTGTGAT TCCACAACAG CAGCAGACTA CTCAGAACCA ACCTTCCAGA 
ATGGACCGGA GTGGAATGGG GGTAGGGGCT GGTGGTGTAC CAAAAGGCGG TGCTCGTAGA 
GGAAAGGGGC CCTTGGTGAA ACAGTCAAAG GTGGAAGACC TGCGTCAAGG TCAAGACACT 
CTAGAAGCAA AGGTGTCAGT GGAGAAGAGC TCTATTCCAA TCCCAACTAT CATTGTTAAA 
GCCCCTTCCA CCAGCAGTAG TGGACGCAGT AGCCAAGGCA GCAGTGTGGA GGCTGAACCA 
CCGCCCCCAG GAGAAAGTGA CAGTTCTAAA CCAGAACCTG TTGTTCCCCC ACCTGCACCA 
ACCGTGCCAC CACCAGCCCC TCCATCTATA CCTGCTCCTC CACCCCCATC ACTGCCATCA 
CTTCGTACTC AAGAGAGTCT GGACTTCACC AGCCAGTTTG GTGCTGCCAT TGTAGGGGCA 
GCTCGCAGGG ACAGAGAACG ATTTCATGAA GCACGACGCA AGAGTGCCTC CTTTTTTCTT 
TCTGCTGAGG AAGAGTTGAG TGGAGCTGGA GGAGCAGGAG GGCGGACTCA GACTTCTCAA 
CAGCAGCTGA GTTCCCAGCC TTCACCACGC CTGCGACCAT CCAAATCTAT AGATGAAGGC 
ATGTTCTCAG GTGATACTTT TATCCATCAC ACACGCAGTA TGCCTCCTGC ATTTGGCTTG 
CCTGAGTACT CCTCACCTGC TCCTCAAGGG GATTACCAGC CTAAGTCTGT ACCTGCTGAC 
TTCTATGGGG CAGGATCCAG ACAACAGCAG CAGCAGCAGC AGCAGCAAGC CCCCACCACA 
ACTTTTATTC ACCCACTAAC TGGAAAAGTA CTGGACCCTT CATCCCCTCT AGGCTTGGCT 
TTGGCTGCAC GGGAACGTGC CTTGAAGGAT GATGGACGTA TACGTAGGGA AAGGACCACT 
GAACATCATT TTACCCGTCA GTTGTCAAGC ATTGGGGCCT TTTCCTCATC CACTTCACAG 
CCAACATCAC ACATTTCCTA CCCAATGGCC TCGTCTTCCT CTGCAACCTC CTCTGCTGCT 
CAATCCACAA ACACCTCTTC ATATCTTGTT ACCTCCTCAT CATTAGCTGC CACCACCACA 
TCCACTCGGC CTCCATCTCC TAGGATCCTG CGTGGAGCAG GGAGTAGCTG GAGTGAAGAA 
GCTGGGGTTG GAGAGCGTGC GGATAGGGAA GCAGCTGGCA CCTCTGCTCC CAGAGAAGGC 
CTAAGGGTTC GTTTCTCCGA GGATAAAACT GTCCACACAC ACCACTATCA ATCTCAGCAA 
TATCAGCCCA CCTTTAAAGA GCGTGAAAGA GAACGTCAAA GAGAGAGGTC AAGAGAAAGA 
GAGAGGGAAA GAGAGAGGGA AAGAGAAATA GAAAAAGCTC AAACCCAGTA TCAGCAACAG 
TCATCACAAC CAATGACCCA GCAGCCTCGA CGCCCCTCAT TCTTGAGGAT GGAGAGTGAA 
GCTGGAGCGT ACATTCTTTC ATTAACGCCT CCATCTCCTC CTCCAACAGC AGCCCCTATG 
CGTCCTACAG CAGGAGCCAC AGGTGAAAGT GGAAGTGGTA CAAGCCTGAT GGTTCTGCCT 
CCCCCAGCTC CCTCAGTAGA TGCAGATGAT GAGTTTGTGT TTGCTGATCC TCTTCCTCCA 
CCTCTAGAGT TTGCCAATAG TTTTGATCGT GGTTTGGGTT ACTCTGCGAT GATTTCTCAC 
AATGTTGCAG CCCGTCAGCA GCGTCATCCT CCACCACCCC CTCCCCCACC ACCCCCGCCC 
ACTTCGAAAG ATTCCTTTAT GACAGGCTTT CCAGCTCACA CACCCCTACC GTCACCTCAG 
GCAGGAGATT CCACAACTTC CAGTCTTACC TCCTATGACA GTGAGGTTGC AAACCTAACA 
CAATCTGCCC CATCGCCCTC CCCATCTTCT CCCAACCCAC TACCACCTCA CTCCCCCTCC 
ACCCTTCAGA CATCTGGACC ACCTCCACCT CCCTCTGTAT CACCTCCTCC ACCACCCAAC 
AGCTACCATA GACCTTTTGG CTTATCTCAG TCACAACATT TATACAACAG TACACACACA 
ACTGGACGCT CCTCCTCTCC CACCCCACCT CTCTCTATTC CAGCTCCACC TGCCTACCGG 
GGGCCACCAA CAGCATCATC CACCCCTGCA ACTTCTGTAT CAATCAGAGG TGCCTCAACT 
GTGACCGCAA GCTGTTCCAC AACTGCTTCT ACAACTGTCA CCTCTCCTAG TACTACTGCT 
GCTGCCACAC CACACACACG CATAGCAACT GTCTCCACAG TAGCTACAGG GGGTAGTAGG 
CGTCCCAGTG CAGAGCATCA TCCACCACCT AGTGCAACCA AGAGTGGTGA GTCCCAGGAG 
ACAGTGGTAG ATTCTGGGAT TGAGGAGCTG GACAGCCGAA GCAGCAGTGA TCATCACTTA 
GACAGCATTC TTGCACTCAG TGGTAGTGGG GTCAGAGAGA GGTTGGAACG AGCAGGGATG 
AGTAGCATAG GTGGAATTGG AGGGAGAGAT GGTGCAACTG AGGTTGACAG GACAAGTGGG 
GCAGACTTTT TAGAGTCTTA CATGAGCTAC CTTGATGGGC AGACTTTTGA AATGCACAAT 
GCCAAAGCCG CTAGCACTGC ATCCACCTAC CCAAAAACAC AGAGGTACAG AGAAGGCACC 
CGGGCTGGAG ATCTTCGACG CCAGACCAGC ACAGCACCTC CTGCATACCA CCGTCGTAGA 
GAGGAGGAAG AGGAAGAGGA TGAGGCCGAG GAGGTAGAGG AGGAGGATTT GAGTGCCCGT 
GATGGCTATG GGATGAGGGA AGGGCCAGGG ATTGCTCGCT CCTCTGTAAT GTACTTTGAC 
CGGCCCCGCA CTCCAGAGAT AAAACCACTG TGGGGAGATG GGTCGACTGG AGCATCACAG 
ACCATGGGGG AGGGAGGGGG CCAGATAGTG GGAACATATG TGGAGCCACG TAAACTACAT 
CCTCCCATGA CTGGTATGAA AGCAAACATC ATTAATGAAC TAAGCACCAA ATTACAGCAG 
AGAAGTAAAG GAACAGAGAA CTGGGGGGCC CAGAGATCAT TGAGCAGACA TAGACACAGG 
TTCTCAGAGG ACTCAACAGG TGCACTGAGT CCTCCCTCTG TCCGCTCTAG CTCCCCATCT 
CCAGTTCAGC TCTCTCAGCC TGCCTTGTCT CCCTCTCCCA CTCCTTCCTC CCAGCCACTT 
TACTCAAACT GGGCTCGATC TCCTTCCCCT CAGCCCCCTG CTGCCTCATC CCAGCCCCCT 
CCCCGATCCC ATTCACCTGT TTCCCCTTCA TCATATTCAC CATACCCCAC CTCACCCAAA 
CACCGGCCTG TTTATCGCAC CAAAGCTCTG GAGTTCCAAT TTATTCCCAG TCGAGAGTCA 
CGAAAGTCAG AGTCGCATCA TGCCCAGCGC AGACGAGCAC CCAGCCCTTT AATTTCTCCA 
TCTGACCGTC CCAAGCTTGG CCCTCCCCGC CCCTCTTCCC TTCCTATTCT CCCCACTACA 
CCCCTCTATA GCAGCCTGTA TGACCTTCGT GGTTCTATTA CTCCACCCTC ACCTGCCAAT 
CCCCTTGGGG ACCCCTACTT TTCCCCCTCC CCCCCTCTAT TTGCCCCCTC GGGTGCTCCC 
CCTCCTCCAA ATTCCTCTCT GGTGGTCTCA CGGTCCCTGT CACCCACTCA CTTCCTGTCA 
GGGACTTCAT CCCCACCTCT CCACCCCATT CCTCCACCCA CTTGCCTGAG TTACCCCCAT 
CTTCCACCCC CCTCACCCAC AAAGCCCTTT GCCTCAAAAC CTCTCCCATA CTGGACCAAG 
TATGATGTTG CAGATTGGCT GGGCTACCTG AACCTGGGTG AACACAGGGA GCGATTCTTA 
GACAATGAAA TTGATGGCAC GCACCTGCCC TCTCTTACCA AGGATGACTA CCTGGACCTG 
GGTGTGACCC GAGTTGGCCA TCGCATGAAC ATAGAGAGGG CGCTCAGGAG AGTTATGGAC 
AGGCTGTCTT CTAGCACTTT CCCCATTTCT GCTCTCTCTC CTCGAAATGG ACGGACAAAC 
AGAAGGAGAG ATGAGGCCAA CCGGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_021471433.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327108.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X3, mRNA.

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CloneID	ODa141765
Clone ID Related Accession (Same CDS sequence)	XM_021471433.1 , XM_021474062.1
Accession Version	XM_021471433.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394533.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGTG 
GACAGAGCCT CTGCCCCATG GAAAAAAAAA TCAGAGTTCG AATCTTCACA AGCTACAGAG 
AAGAAGAGGA CAGTTTATCA GATGGCTTTG AATAAACTGG ATGAGATTTT AGCAGCAGCA 
CAGCAGACCA TTAATACAAA TGAGGCTCCA GGGCCCCGGG TTCAGGGGGT AAAAAGAGAA 
CGGGGCAGAG GCTTTTATAA TGAGCCAAAT TTCGACCAAA CTGGAATGGG GATGACATCA 
TCTGGGGCAG GGTTAGGCTA CGACCGTGGT CAATTTGTGT CTGGCCATGG CCCCCCGCAC 
GGTATGCTGC GACAGAAGTC TATCGGGGTT CCTGAGGAGG AGAAGCAGTT TCTTCATCCT 
CCAGCCATGA AGTTTGCCCG TAGTCTTTCA GTACCTGGCC CAGATGAAAT CCCTCCTCCA 
CCTACTACAG CACCCCCTGA GCCCCCTTTT TCCTCAGCTC CTCCGCTTGG CTTTAGGGGC 
AAGACATCTC AACAGCCGTC TGTTTCTATG TCTCAGTCTA CTTCCTCTGC TGCACATTAT 
CAACTCTACT CACAGTCAGT ACATGTTACT CACGGACCAA GGGACAGGTC CCCTGGACCA 
CCTACTGGGT CTGGAACTGG GGACCTTGCT TATTCCCATG CCCAAGCCCA CCCCTCTGTA 
CCAAGTAGGA CTGCCTCCAG GAAGGTGATT GGGTACACAG GAGATCTTGC AGGGGCTGGA 
GGAGTGGCTG GGGCCAAAGC TGCAGCATTG AGAAGGGGTT ACAGCAATGC AGTCCCACCC 
ACCAGTGTCG CTACTGTGAT TCCACAACAG CAGCAGACTA CTCAGAACCA ACCTTCCAGA 
ATGGACCGGA GTGGAATGGG GGTAGGGGCT GGTGGTGTAC CAAAAGGCGG TGCTCGTAGA 
GGAAAGGGGC CCTTGGTGAA ACAGTCAAAG GTGGAAGACC TGCGTCAAGG TCAAGACACT 
CTAGAAGCAA AGGTGTCAGT GGAGAAGAGC TCTATTCCAA TCCCAACTAT CATTGTTAAA 
GCCCCTTCCA CCAGCAGTAG TGGACGCAGT AGCCAAGGCA GCAGTGTGGA GGCTGAACCA 
CCGCCCCCAG GAGAAAGTGA CAGTTCTAAA CCAGAACCTG TTGTTCCCCC ACCTGCACCA 
ACCGTGCCAC CACCAGCCCC TCCATCTATA CCTGCTCCTC CACCCCCATC ACTGCCATCA 
CTTCGTACTC AAGAGAGTCT GGACTTCACC AGCCAGTTTG GTGCTGCCAT TGTAGGGGCA 
GCTCGCAGGG ACAGAGAACG ATTTCATGAA GCACGACGCA AGAGTGCCTC CTTTTTTCTT 
TCTGCTGAGG AAGAGTTGAG TGGAGCTGGA GGAGCAGGAG GGCGGACTCA GACTTCTCAA 
CAGCAGCTGA GTTCCCAGCC TTCACCACGC CTGCGACCAT CCAAATCTAT AGATGAAGGC 
ATGTTCTCAG GTGATACTTT TATCCATCAC ACACGCAGTA TGCCTCCTGC ATTTGGCTTG 
CCTGAGTACT CCTCACCTGC TCCTCAAGGG GATTACCAGC CTAAGTCTGT ACCTGCTGAC 
TTCTATGGGG CAGGATCCAG ACAACAGCAG CAGCAGCAGC AGCAGCAAGC CCCCACCACA 
ACTTTTATTC ACCCACTAAC TGGAAAAGTA CTGGACCCTT CATCCCCTCT AGGCTTGGCT 
TTGGCTGCAC GGGAACGTGC CTTGAAGGAT GATGGACGTA TACGTAGGGA AAGGACCACT 
GAACATCATT TTACCCGTCA GTTGTCAAGC ATTGGGGCCT TTTCCTCATC CACTTCACAG 
CCAACATCAC ACATTTCCTA CCCAATGGCC TCGTCTTCCT CTGCAACCTC CTCTGCTGCT 
CAATCCACAA ACACCTCTTC ATATCTTGTT ACCTCCTCAT CATTAGCTGC CACCACCACA 
TCCACTCGGC CTCCATCTCC TAGGATCCTG CGTGGAGCAG GGAGTAGCTG GAGTGAAGAA 
GCTGGGGTTG GAGAGCGTGC GGATAGGGAA GCAGCTGGCA CCTCTGCTCC CAGAGAAGGC 
CTAAGGGTTC GTTTCTCCGA GGATAAAACT GTCCACACAC ACCACTATCA ATCTCAGCAA 
TATCAGCCCA CCTTTAAAGA GCGTGAAAGA GAACGTCAAA GAGAGAGGTC AAGAGAAAGA 
GAGAGGGAAA GAGAGAGGGA AAGAGAAATA GAAAAAGCTC AAACCCAGTA TCAGCAACAG 
TCATCACAAC CAATGACCCA GCAGCCTCGA CGCCCCTCAT TCTTGAGGAT GGAGAGTGAA 
GCTGGAGCGT ACATTCTTTC ATTAACGCCT CCATCTCCTC CTCCAACAGC AGCCCCTATG 
CGTCCTACAG CAGGAGCCAC AGGTGAAAGT GGAAGTGGTA CAAGCCTGAT GGTTCTGCCT 
CCCCCAGCTC CCTCAGTAGA TGCAGATGAT GAGTTTGTGT TTGCTGATCC TCTTCCTCCA 
CCTCTAGAGT TTGCCAATAG TTTTGATCGT GGTTTGGGTT ACTCTGCGAT GATTTCTCAC 
AATGTTGCAG CCCGTCAGCA GCGTCATCCT CCACCACCCC CTCCCCCACC ACCCCCGCCC 
ACTTCGAAAG ATTCCTTTAT GACAGGCTTT CCAGCTCACA CACCCCTACC GTCACCTCAG 
GCAGGAGATT CCACAACTTC CAGTCTTACC TCCTATGACA GTGAGGTTGC AAACCTAACA 
CAATCTGCCC CATCGCCCTC CCCATCTTCT CCCAACCCAC TACCACCTCA CTCCCCCTCC 
ACCCTTCAGA CATCTGGACC ACCTCCACCT CCCTCTGTAT CACCTCCTCC ACCACCCAAC 
AGCTACCATA GACCTTTTGG CTTATCTCAG TCACAACATT TATACAACAG TACACACACA 
ACTGGACGCT CCTCCTCTCC CACCCCACCT CTCTCTATTC CAGCTCCACC TGCCTACCGG 
GGGCCACCAA CAGCATCATC CACCCCTGCA ACTTCTGTAT CAATCAGAGG TGCCTCAACT 
GTGACCGCAA GCTGTTCCAC AACTGCTTCT ACAACTGTCA CCTCTCCTAG TACTACTGCT 
GCTGCCACAC CACACACACG CATAGCAACT GTCTCCACAG TAGCTACAGG GGGTAGTAGG 
CGTCCCAGTG CAGAGCATCA TCCACCACCT AGTGCAACCA AGAGTGGTGA GTCCCAGGAG 
ACAGTGGTAG ATTCTGGGAT TGAGGAGCTG GACAGCCGAA GCAGCAGTGA TCATCACTTA 
GACAGCATTC TTGCACTCAG TGGTAGTGGG GTCAGAGAGA GGTTGGAACG AGCAGGGATG 
AGTAGCATAG GTGGAATTGG AGGGAGAGAT GGTGCAACTG AGGTTGACAG GACAAGTGGG 
GCAGACTTTT TAGAGTCTTA CATGAGCTAC CTTGATGGGC AGACTTTTGA AATGCACAAT 
GCCAAAGCCG CTAGCACTGC ATCCACCTAC CCAAAAACAC AGAGGTACAG AGAAGGCACC 
CGGGCTGGAG ATCTTCGACG CCAGACCAGC ACAGCACCTC CTGCATACCA CCGTCGTAGA 
GAGGAGGAAG AGGAAGAGGA TGAGGCCGAG GAGGTAGAGG AGGAGGATTT GAGTGCCCGT 
GATGGCTATG GGATGAGGGA AGGGCCAGGG ATTGCTCGCT CCTCTGTAAT GTACTTTGAC 
CGGCCCCGCA CTCCAGAGAT AAAACCACTG TGGGGAGATG GGTCGACTGG AGCATCACAG 
ACCATGGGGG AGGGAGGGGG CCAGATAGTG GGAACATATG TGGAGCCACG TAAACTACAT 
CCTCCCATGA CTGGTATGAA AGCAAACATC ATTAATGAAC TAAGCACCAA ATTACAGCAG 
AGAAGTAAAG GAACAGAGAA CTGGGGGGCC CAGAGATCAT TGAGCAGACA TAGGTTCTCA 
GAGGACTCAA CAGGTGCACT GAGTCCTCCC TCTGTCCGCT CTAGCTCCCC ATCTCCAGTT 
CAGCTCTCTC AGCCTGCCTT GTCTCCCTCT CCCACTCCTT CCTCCCAGCC ACTTTACTCA 
AACTGGGCTC GATCTCCTTC CCCTCAGCCC CCTGCTGCCT CATCCCAGCC CCCTCCCCGA 
TCCCATTCAC CTGTTTCCCC TTCATCATAT TCACCATACC CCACCTCACC CAAACACCGG 
CCTGTTTATC GCACCAAAGC TCTGGAGTTC CAATTTATTC CCAGTCGAGA GTCACGAAAG 
TCAGAGTCGC ATCATGCCCA GCGCAGACGA GCACCCAGCC CTTTAATTTC TCCATCTGAC 
CGTCCCAAGC TTGGCCCTCC CCGCCCCTCT TCCCTTCCTA TTCTCCCCAC TACACCCCTC 
TATAGCAGCC TGTATGACCT TCGTGGTTCT ATTACTCCAC CCTCACCTGC CAATCCCCTT 
GGGGACCCCT ACTTTTCCCC CTCCCCCCCT CTATTTGCCC CCTCGGGTGC TCCCCCTCCT 
CCAAATTCCT CTCTGGTGGT CTCACGGTCC CTGTCACCCA CTCACTTCCT GTCAGGGACT 
TCATCCCCAC CTCTCCACCC CATTCCTCCA CCCACTTGCC TGAGTTACCC CCATCTTCCA 
CCCCCCTCAC CCACAAAGCC CTTTGCCTCA AAACCTCTCC CATACTGGAC CAAGTATGAT 
GTTGCAGATT GGCTGGGCTA CCTGAACCTG GGTGAACACA GGGAGCGATT CTTAGACAAT 
GAAATTGATG GCACGCACCT GCCCTCTCTT ACCAAGGATG ACTACCTGGA CCTGGGTGTG 
ACCCGAGTTG GCCATCGCAT GAACATAGAG AGGGCGCTCA GGAGAGTTAT GGACAGGCTG 
TCTTCTAGCA CTTTCCCCAT TTCTGCTCTC TCTCCTCGAA ATGGACGGAC AAACAGAAGG 
AGAGATGAGG CCAACCGGAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327108.1
CDS	381..7784
Translation	MTMPLSPLSSDEEQQRMLGNSQHLYPGAEEEEEEEEEMDDDGMDEEGEEENGELEMDDEEEVDDEDDIRRGPGHLRGGREEGHRQKGTMAGRLPGDRPIRPGNPGHAANPYSSNPGPTHQTSTNQQQQQPQRKLRERSSSNAPDDTHVALMVFRIGIPDIKQTKCLRFNPDATVWKAKQQVMCSLTESLRDVLNYGLFQPATDGHDAKFLEEERPLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKASLKKFMDYIQTSAVEKVAKLIDKGLDPNYHDPDTGETPLTLAVQTEPGGSEIIRVLVMGGAHIDFRARDGLTPLHKAVRAHAHTALLTLLSLGASSDYKDSRGLTPLYHTVLIGGDTSCCETLLYHRARLGLRDENGWDETHQACQNGNSQHLEHLLFYGADSSSQNASGNTALHICALYNKESCARILLYRGAKKDTKNNSGQNSFQVAVMSGHFELGEIIKNHRESDVVPFLESPKYAPQRRESSRTLGLPHPHPFLRANSDNSMNVPDWIAFPNAAGSSIVSVQGLKHGGTLRSSSSPRGARTRSPSRGRTGDRDDRSRQTRGRQGAGSISSQGTASGQRRRLYSAVPGRVFVATRSHSAQGDREISFNKGDKVKVLSVGEGGYWEGTVRGRTGWFPSDCVEEVALRSLESRSESRSERAKRLFRHYTVGSYDSFDAPSDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMVDRASAPWKKKSEFESSQATEKKRTVYQMALNKLDEILAAAQQTINTNEAPGPRVQGVKRERGRGFYNEPNFDQTGMGMTSSGAGLGYDRGQFVSGHGPPHGMLRQKSIGVPEEEKQFLHPPAMKFARSLSVPGPDEIPPPPTTAPPEPPFSSAPPLGFRGKTSQQPSVSMSQSTSSAAHYQLYSQSVHVTHGPRDRSPGPPTGSGTGDLAYSHAQAHPSVPSRTASRKVIGYTGDLAGAGGVAGAKAAALRRGYSNAVPPTSVATVIPQQQQTTQNQPSRMDRSGMGVGAGGVPKGGARRGKGPLVKQSKVEDLRQGQDTLEAKVSVEKSSIPIPTIIVKAPSTSSSGRSSQGSSVEAEPPPPGESDSSKPEPVVPPPAPTVPPPAPPSIPAPPPPSLPSLRTQESLDFTSQFGAAIVGAARRDRERFHEARRKSASFFLSAEEELSGAGGAGGRTQTSQQQLSSQPSPRLRPSKSIDEGMFSGDTFIHHTRSMPPAFGLPEYSSPAPQGDYQPKSVPADFYGAGSRQQQQQQQQQAPTTTFIHPLTGKVLDPSSPLGLALAARERALKDDGRIRRERTTEHHFTRQLSSIGAFSSSTSQPTSHISYPMASSSSATSSAAQSTNTSSYLVTSSSLAATTTSTRPPSPRILRGAGSSWSEEAGVGERADREAAGTSAPREGLRVRFSEDKTVHTHHYQSQQYQPTFKERERERQRERSREREREREREREIEKAQTQYQQQSSQPMTQQPRRPSFLRMESEAGAYILSLTPPSPPPTAAPMRPTAGATGESGSGTSLMVLPPPAPSVDADDEFVFADPLPPPLEFANSFDRGLGYSAMISHNVAARQQRHPPPPPPPPPPPTSKDSFMTGFPAHTPLPSPQAGDSTTSSLTSYDSEVANLTQSAPSPSPSSPNPLPPHSPSTLQTSGPPPPPSVSPPPPPNSYHRPFGLSQSQHLYNSTHTTGRSSSPTPPLSIPAPPAYRGPPTASSTPATSVSIRGASTVTASCSTTASTTVTSPSTTAAATPHTRIATVSTVATGGSRRPSAEHHPPPSATKSGESQETVVDSGIEELDSRSSSDHHLDSILALSGSGVRERLERAGMSSIGGIGGRDGATEVDRTSGADFLESYMSYLDGQTFEMHNAKAASTASTYPKTQRYREGTRAGDLRRQTSTAPPAYHRRREEEEEEDEAEEVEEEDLSARDGYGMREGPGIARSSVMYFDRPRTPEIKPLWGDGSTGASQTMGEGGGQIVGTYVEPRKLHPPMTGMKANIINELSTKLQQRSKGTENWGAQRSLSRHRFSEDSTGALSPPSVRSSSPSPVQLSQPALSPSPTPSSQPLYSNWARSPSPQPPAASSQPPPRSHSPVSPSSYSPYPTSPKHRPVYRTKALEFQFIPSRESRKSESHHAQRRRAPSPLISPSDRPKLGPPRPSSLPILPTTPLYSSLYDLRGSITPPSPANPLGDPYFSPSPPLFAPSGAPPPPNSSLVVSRSLSPTHFLSGTSSPPLHPIPPPTCLSYPHLPPPSPTKPFASKPLPYWTKYDVADWLGYLNLGEHRERFLDNEIDGTHLPSLTKDDYLDLGVTRVGHRMNIERALRRVMDRLSSSTFPISALSPRNGRTNRRRDEANRS

Target ORF information:

RefSeq Version	XM_021471433.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471433.1

ORF Insert Sequence:

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGTG 
GACAGAGCCT CTGCCCCATG GAAAAAAAAA TCAGAGTTCG AATCTTCACA AGCTACAGAG 
AAGAAGAGGA CAGTTTATCA GATGGCTTTG AATAAACTGG ATGAGATTTT AGCAGCAGCA 
CAGCAGACCA TTAATACAAA TGAGGCTCCA GGGCCCCGGG TTCAGGGGGT AAAAAGAGAA 
CGGGGCAGAG GCTTTTATAA TGAGCCAAAT TTCGACCAAA CTGGAATGGG GATGACATCA 
TCTGGGGCAG GGTTAGGCTA CGACCGTGGT CAATTTGTGT CTGGCCATGG CCCCCCGCAC 
GGTATGCTGC GACAGAAGTC TATCGGGGTT CCTGAGGAGG AGAAGCAGTT TCTTCATCCT 
CCAGCCATGA AGTTTGCCCG TAGTCTTTCA GTACCTGGCC CAGATGAAAT CCCTCCTCCA 
CCTACTACAG CACCCCCTGA GCCCCCTTTT TCCTCAGCTC CTCCGCTTGG CTTTAGGGGC 
AAGACATCTC AACAGCCGTC TGTTTCTATG TCTCAGTCTA CTTCCTCTGC TGCACATTAT 
CAACTCTACT CACAGTCAGT ACATGTTACT CACGGACCAA GGGACAGGTC CCCTGGACCA 
CCTACTGGGT CTGGAACTGG GGACCTTGCT TATTCCCATG CCCAAGCCCA CCCCTCTGTA 
CCAAGTAGGA CTGCCTCCAG GAAGGTGATT GGGTACACAG GAGATCTTGC AGGGGCTGGA 
GGAGTGGCTG GGGCCAAAGC TGCAGCATTG AGAAGGGGTT ACAGCAATGC AGTCCCACCC 
ACCAGTGTCG CTACTGTGAT TCCACAACAG CAGCAGACTA CTCAGAACCA ACCTTCCAGA 
ATGGACCGGA GTGGAATGGG GGTAGGGGCT GGTGGTGTAC CAAAAGGCGG TGCTCGTAGA 
GGAAAGGGGC CCTTGGTGAA ACAGTCAAAG GTGGAAGACC TGCGTCAAGG TCAAGACACT 
CTAGAAGCAA AGGTGTCAGT GGAGAAGAGC TCTATTCCAA TCCCAACTAT CATTGTTAAA 
GCCCCTTCCA CCAGCAGTAG TGGACGCAGT AGCCAAGGCA GCAGTGTGGA GGCTGAACCA 
CCGCCCCCAG GAGAAAGTGA CAGTTCTAAA CCAGAACCTG TTGTTCCCCC ACCTGCACCA 
ACCGTGCCAC CACCAGCCCC TCCATCTATA CCTGCTCCTC CACCCCCATC ACTGCCATCA 
CTTCGTACTC AAGAGAGTCT GGACTTCACC AGCCAGTTTG GTGCTGCCAT TGTAGGGGCA 
GCTCGCAGGG ACAGAGAACG ATTTCATGAA GCACGACGCA AGAGTGCCTC CTTTTTTCTT 
TCTGCTGAGG AAGAGTTGAG TGGAGCTGGA GGAGCAGGAG GGCGGACTCA GACTTCTCAA 
CAGCAGCTGA GTTCCCAGCC TTCACCACGC CTGCGACCAT CCAAATCTAT AGATGAAGGC 
ATGTTCTCAG GTGATACTTT TATCCATCAC ACACGCAGTA TGCCTCCTGC ATTTGGCTTG 
CCTGAGTACT CCTCACCTGC TCCTCAAGGG GATTACCAGC CTAAGTCTGT ACCTGCTGAC 
TTCTATGGGG CAGGATCCAG ACAACAGCAG CAGCAGCAGC AGCAGCAAGC CCCCACCACA 
ACTTTTATTC ACCCACTAAC TGGAAAAGTA CTGGACCCTT CATCCCCTCT AGGCTTGGCT 
TTGGCTGCAC GGGAACGTGC CTTGAAGGAT GATGGACGTA TACGTAGGGA AAGGACCACT 
GAACATCATT TTACCCGTCA GTTGTCAAGC ATTGGGGCCT TTTCCTCATC CACTTCACAG 
CCAACATCAC ACATTTCCTA CCCAATGGCC TCGTCTTCCT CTGCAACCTC CTCTGCTGCT 
CAATCCACAA ACACCTCTTC ATATCTTGTT ACCTCCTCAT CATTAGCTGC CACCACCACA 
TCCACTCGGC CTCCATCTCC TAGGATCCTG CGTGGAGCAG GGAGTAGCTG GAGTGAAGAA 
GCTGGGGTTG GAGAGCGTGC GGATAGGGAA GCAGCTGGCA CCTCTGCTCC CAGAGAAGGC 
CTAAGGGTTC GTTTCTCCGA GGATAAAACT GTCCACACAC ACCACTATCA ATCTCAGCAA 
TATCAGCCCA CCTTTAAAGA GCGTGAAAGA GAACGTCAAA GAGAGAGGTC AAGAGAAAGA 
GAGAGGGAAA GAGAGAGGGA AAGAGAAATA GAAAAAGCTC AAACCCAGTA TCAGCAACAG 
TCATCACAAC CAATGACCCA GCAGCCTCGA CGCCCCTCAT TCTTGAGGAT GGAGAGTGAA 
GCTGGAGCGT ACATTCTTTC ATTAACGCCT CCATCTCCTC CTCCAACAGC AGCCCCTATG 
CGTCCTACAG CAGGAGCCAC AGGTGAAAGT GGAAGTGGTA CAAGCCTGAT GGTTCTGCCT 
CCCCCAGCTC CCTCAGTAGA TGCAGATGAT GAGTTTGTGT TTGCTGATCC TCTTCCTCCA 
CCTCTAGAGT TTGCCAATAG TTTTGATCGT GGTTTGGGTT ACTCTGCGAT GATTTCTCAC 
AATGTTGCAG CCCGTCAGCA GCGTCATCCT CCACCACCCC CTCCCCCACC ACCCCCGCCC 
ACTTCGAAAG ATTCCTTTAT GACAGGCTTT CCAGCTCACA CACCCCTACC GTCACCTCAG 
GCAGGAGATT CCACAACTTC CAGTCTTACC TCCTATGACA GTGAGGTTGC AAACCTAACA 
CAATCTGCCC CATCGCCCTC CCCATCTTCT CCCAACCCAC TACCACCTCA CTCCCCCTCC 
ACCCTTCAGA CATCTGGACC ACCTCCACCT CCCTCTGTAT CACCTCCTCC ACCACCCAAC 
AGCTACCATA GACCTTTTGG CTTATCTCAG TCACAACATT TATACAACAG TACACACACA 
ACTGGACGCT CCTCCTCTCC CACCCCACCT CTCTCTATTC CAGCTCCACC TGCCTACCGG 
GGGCCACCAA CAGCATCATC CACCCCTGCA ACTTCTGTAT CAATCAGAGG TGCCTCAACT 
GTGACCGCAA GCTGTTCCAC AACTGCTTCT ACAACTGTCA CCTCTCCTAG TACTACTGCT 
GCTGCCACAC CACACACACG CATAGCAACT GTCTCCACAG TAGCTACAGG GGGTAGTAGG 
CGTCCCAGTG CAGAGCATCA TCCACCACCT AGTGCAACCA AGAGTGGTGA GTCCCAGGAG 
ACAGTGGTAG ATTCTGGGAT TGAGGAGCTG GACAGCCGAA GCAGCAGTGA TCATCACTTA 
GACAGCATTC TTGCACTCAG TGGTAGTGGG GTCAGAGAGA GGTTGGAACG AGCAGGGATG 
AGTAGCATAG GTGGAATTGG AGGGAGAGAT GGTGCAACTG AGGTTGACAG GACAAGTGGG 
GCAGACTTTT TAGAGTCTTA CATGAGCTAC CTTGATGGGC AGACTTTTGA AATGCACAAT 
GCCAAAGCCG CTAGCACTGC ATCCACCTAC CCAAAAACAC AGAGGTACAG AGAAGGCACC 
CGGGCTGGAG ATCTTCGACG CCAGACCAGC ACAGCACCTC CTGCATACCA CCGTCGTAGA 
GAGGAGGAAG AGGAAGAGGA TGAGGCCGAG GAGGTAGAGG AGGAGGATTT GAGTGCCCGT 
GATGGCTATG GGATGAGGGA AGGGCCAGGG ATTGCTCGCT CCTCTGTAAT GTACTTTGAC 
CGGCCCCGCA CTCCAGAGAT AAAACCACTG TGGGGAGATG GGTCGACTGG AGCATCACAG 
ACCATGGGGG AGGGAGGGGG CCAGATAGTG GGAACATATG TGGAGCCACG TAAACTACAT 
CCTCCCATGA CTGGTATGAA AGCAAACATC ATTAATGAAC TAAGCACCAA ATTACAGCAG 
AGAAGTAAAG GAACAGAGAA CTGGGGGGCC CAGAGATCAT TGAGCAGACA TAGGTTCTCA 
GAGGACTCAA CAGGTGCACT GAGTCCTCCC TCTGTCCGCT CTAGCTCCCC ATCTCCAGTT 
CAGCTCTCTC AGCCTGCCTT GTCTCCCTCT CCCACTCCTT CCTCCCAGCC ACTTTACTCA 
AACTGGGCTC GATCTCCTTC CCCTCAGCCC CCTGCTGCCT CATCCCAGCC CCCTCCCCGA 
TCCCATTCAC CTGTTTCCCC TTCATCATAT TCACCATACC CCACCTCACC CAAACACCGG 
CCTGTTTATC GCACCAAAGC TCTGGAGTTC CAATTTATTC CCAGTCGAGA GTCACGAAAG 
TCAGAGTCGC ATCATGCCCA GCGCAGACGA GCACCCAGCC CTTTAATTTC TCCATCTGAC 
CGTCCCAAGC TTGGCCCTCC CCGCCCCTCT TCCCTTCCTA TTCTCCCCAC TACACCCCTC 
TATAGCAGCC TGTATGACCT TCGTGGTTCT ATTACTCCAC CCTCACCTGC CAATCCCCTT 
GGGGACCCCT ACTTTTCCCC CTCCCCCCCT CTATTTGCCC CCTCGGGTGC TCCCCCTCCT 
CCAAATTCCT CTCTGGTGGT CTCACGGTCC CTGTCACCCA CTCACTTCCT GTCAGGGACT 
TCATCCCCAC CTCTCCACCC CATTCCTCCA CCCACTTGCC TGAGTTACCC CCATCTTCCA 
CCCCCCTCAC CCACAAAGCC CTTTGCCTCA AAACCTCTCC CATACTGGAC CAAGTATGAT 
GTTGCAGATT GGCTGGGCTA CCTGAACCTG GGTGAACACA GGGAGCGATT CTTAGACAAT 
GAAATTGATG GCACGCACCT GCCCTCTCTT ACCAAGGATG ACTACCTGGA CCTGGGTGTG 
ACCCGAGTTG GCCATCGCAT GAACATAGAG AGGGCGCTCA GGAGAGTTAT GGACAGGCTG 
TCTTCTAGCA CTTTCCCCAT TTCTGCTCTC TCTCCTCGAA ATGGACGGAC AAACAGAAGG 
AGAGATGAGG CCAACCGGAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_021474062.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329737.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X3, mRNA.

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CloneID	ODa141765
Clone ID Related Accession (Same CDS sequence)	XM_021471433.1 , XM_021474062.1
Accession Version	XM_021474062.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGTG 
GACAGAGCCT CTGCCCCATG GAAAAAAAAA TCAGAGTTCG AATCTTCACA AGCTACAGAG 
AAGAAGAGGA CAGTTTATCA GATGGCTTTG AATAAACTGG ATGAGATTTT AGCAGCAGCA 
CAGCAGACCA TTAATACAAA TGAGGCTCCA GGGCCCCGGG TTCAGGGGGT AAAAAGAGAA 
CGGGGCAGAG GCTTTTATAA TGAGCCAAAT TTCGACCAAA CTGGAATGGG GATGACATCA 
TCTGGGGCAG GGTTAGGCTA CGACCGTGGT CAATTTGTGT CTGGCCATGG CCCCCCGCAC 
GGTATGCTGC GACAGAAGTC TATCGGGGTT CCTGAGGAGG AGAAGCAGTT TCTTCATCCT 
CCAGCCATGA AGTTTGCCCG TAGTCTTTCA GTACCTGGCC CAGATGAAAT CCCTCCTCCA 
CCTACTACAG CACCCCCTGA GCCCCCTTTT TCCTCAGCTC CTCCGCTTGG CTTTAGGGGC 
AAGACATCTC AACAGCCGTC TGTTTCTATG TCTCAGTCTA CTTCCTCTGC TGCACATTAT 
CAACTCTACT CACAGTCAGT ACATGTTACT CACGGACCAA GGGACAGGTC CCCTGGACCA 
CCTACTGGGT CTGGAACTGG GGACCTTGCT TATTCCCATG CCCAAGCCCA CCCCTCTGTA 
CCAAGTAGGA CTGCCTCCAG GAAGGTGATT GGGTACACAG GAGATCTTGC AGGGGCTGGA 
GGAGTGGCTG GGGCCAAAGC TGCAGCATTG AGAAGGGGTT ACAGCAATGC AGTCCCACCC 
ACCAGTGTCG CTACTGTGAT TCCACAACAG CAGCAGACTA CTCAGAACCA ACCTTCCAGA 
ATGGACCGGA GTGGAATGGG GGTAGGGGCT GGTGGTGTAC CAAAAGGCGG TGCTCGTAGA 
GGAAAGGGGC CCTTGGTGAA ACAGTCAAAG GTGGAAGACC TGCGTCAAGG TCAAGACACT 
CTAGAAGCAA AGGTGTCAGT GGAGAAGAGC TCTATTCCAA TCCCAACTAT CATTGTTAAA 
GCCCCTTCCA CCAGCAGTAG TGGACGCAGT AGCCAAGGCA GCAGTGTGGA GGCTGAACCA 
CCGCCCCCAG GAGAAAGTGA CAGTTCTAAA CCAGAACCTG TTGTTCCCCC ACCTGCACCA 
ACCGTGCCAC CACCAGCCCC TCCATCTATA CCTGCTCCTC CACCCCCATC ACTGCCATCA 
CTTCGTACTC AAGAGAGTCT GGACTTCACC AGCCAGTTTG GTGCTGCCAT TGTAGGGGCA 
GCTCGCAGGG ACAGAGAACG ATTTCATGAA GCACGACGCA AGAGTGCCTC CTTTTTTCTT 
TCTGCTGAGG AAGAGTTGAG TGGAGCTGGA GGAGCAGGAG GGCGGACTCA GACTTCTCAA 
CAGCAGCTGA GTTCCCAGCC TTCACCACGC CTGCGACCAT CCAAATCTAT AGATGAAGGC 
ATGTTCTCAG GTGATACTTT TATCCATCAC ACACGCAGTA TGCCTCCTGC ATTTGGCTTG 
CCTGAGTACT CCTCACCTGC TCCTCAAGGG GATTACCAGC CTAAGTCTGT ACCTGCTGAC 
TTCTATGGGG CAGGATCCAG ACAACAGCAG CAGCAGCAGC AGCAGCAAGC CCCCACCACA 
ACTTTTATTC ACCCACTAAC TGGAAAAGTA CTGGACCCTT CATCCCCTCT AGGCTTGGCT 
TTGGCTGCAC GGGAACGTGC CTTGAAGGAT GATGGACGTA TACGTAGGGA AAGGACCACT 
GAACATCATT TTACCCGTCA GTTGTCAAGC ATTGGGGCCT TTTCCTCATC CACTTCACAG 
CCAACATCAC ACATTTCCTA CCCAATGGCC TCGTCTTCCT CTGCAACCTC CTCTGCTGCT 
CAATCCACAA ACACCTCTTC ATATCTTGTT ACCTCCTCAT CATTAGCTGC CACCACCACA 
TCCACTCGGC CTCCATCTCC TAGGATCCTG CGTGGAGCAG GGAGTAGCTG GAGTGAAGAA 
GCTGGGGTTG GAGAGCGTGC GGATAGGGAA GCAGCTGGCA CCTCTGCTCC CAGAGAAGGC 
CTAAGGGTTC GTTTCTCCGA GGATAAAACT GTCCACACAC ACCACTATCA ATCTCAGCAA 
TATCAGCCCA CCTTTAAAGA GCGTGAAAGA GAACGTCAAA GAGAGAGGTC AAGAGAAAGA 
GAGAGGGAAA GAGAGAGGGA AAGAGAAATA GAAAAAGCTC AAACCCAGTA TCAGCAACAG 
TCATCACAAC CAATGACCCA GCAGCCTCGA CGCCCCTCAT TCTTGAGGAT GGAGAGTGAA 
GCTGGAGCGT ACATTCTTTC ATTAACGCCT CCATCTCCTC CTCCAACAGC AGCCCCTATG 
CGTCCTACAG CAGGAGCCAC AGGTGAAAGT GGAAGTGGTA CAAGCCTGAT GGTTCTGCCT 
CCCCCAGCTC CCTCAGTAGA TGCAGATGAT GAGTTTGTGT TTGCTGATCC TCTTCCTCCA 
CCTCTAGAGT TTGCCAATAG TTTTGATCGT GGTTTGGGTT ACTCTGCGAT GATTTCTCAC 
AATGTTGCAG CCCGTCAGCA GCGTCATCCT CCACCACCCC CTCCCCCACC ACCCCCGCCC 
ACTTCGAAAG ATTCCTTTAT GACAGGCTTT CCAGCTCACA CACCCCTACC GTCACCTCAG 
GCAGGAGATT CCACAACTTC CAGTCTTACC TCCTATGACA GTGAGGTTGC AAACCTAACA 
CAATCTGCCC CATCGCCCTC CCCATCTTCT CCCAACCCAC TACCACCTCA CTCCCCCTCC 
ACCCTTCAGA CATCTGGACC ACCTCCACCT CCCTCTGTAT CACCTCCTCC ACCACCCAAC 
AGCTACCATA GACCTTTTGG CTTATCTCAG TCACAACATT TATACAACAG TACACACACA 
ACTGGACGCT CCTCCTCTCC CACCCCACCT CTCTCTATTC CAGCTCCACC TGCCTACCGG 
GGGCCACCAA CAGCATCATC CACCCCTGCA ACTTCTGTAT CAATCAGAGG TGCCTCAACT 
GTGACCGCAA GCTGTTCCAC AACTGCTTCT ACAACTGTCA CCTCTCCTAG TACTACTGCT 
GCTGCCACAC CACACACACG CATAGCAACT GTCTCCACAG TAGCTACAGG GGGTAGTAGG 
CGTCCCAGTG CAGAGCATCA TCCACCACCT AGTGCAACCA AGAGTGGTGA GTCCCAGGAG 
ACAGTGGTAG ATTCTGGGAT TGAGGAGCTG GACAGCCGAA GCAGCAGTGA TCATCACTTA 
GACAGCATTC TTGCACTCAG TGGTAGTGGG GTCAGAGAGA GGTTGGAACG AGCAGGGATG 
AGTAGCATAG GTGGAATTGG AGGGAGAGAT GGTGCAACTG AGGTTGACAG GACAAGTGGG 
GCAGACTTTT TAGAGTCTTA CATGAGCTAC CTTGATGGGC AGACTTTTGA AATGCACAAT 
GCCAAAGCCG CTAGCACTGC ATCCACCTAC CCAAAAACAC AGAGGTACAG AGAAGGCACC 
CGGGCTGGAG ATCTTCGACG CCAGACCAGC ACAGCACCTC CTGCATACCA CCGTCGTAGA 
GAGGAGGAAG AGGAAGAGGA TGAGGCCGAG GAGGTAGAGG AGGAGGATTT GAGTGCCCGT 
GATGGCTATG GGATGAGGGA AGGGCCAGGG ATTGCTCGCT CCTCTGTAAT GTACTTTGAC 
CGGCCCCGCA CTCCAGAGAT AAAACCACTG TGGGGAGATG GGTCGACTGG AGCATCACAG 
ACCATGGGGG AGGGAGGGGG CCAGATAGTG GGAACATATG TGGAGCCACG TAAACTACAT 
CCTCCCATGA CTGGTATGAA AGCAAACATC ATTAATGAAC TAAGCACCAA ATTACAGCAG 
AGAAGTAAAG GAACAGAGAA CTGGGGGGCC CAGAGATCAT TGAGCAGACA TAGGTTCTCA 
GAGGACTCAA CAGGTGCACT GAGTCCTCCC TCTGTCCGCT CTAGCTCCCC ATCTCCAGTT 
CAGCTCTCTC AGCCTGCCTT GTCTCCCTCT CCCACTCCTT CCTCCCAGCC ACTTTACTCA 
AACTGGGCTC GATCTCCTTC CCCTCAGCCC CCTGCTGCCT CATCCCAGCC CCCTCCCCGA 
TCCCATTCAC CTGTTTCCCC TTCATCATAT TCACCATACC CCACCTCACC CAAACACCGG 
CCTGTTTATC GCACCAAAGC TCTGGAGTTC CAATTTATTC CCAGTCGAGA GTCACGAAAG 
TCAGAGTCGC ATCATGCCCA GCGCAGACGA GCACCCAGCC CTTTAATTTC TCCATCTGAC 
CGTCCCAAGC TTGGCCCTCC CCGCCCCTCT TCCCTTCCTA TTCTCCCCAC TACACCCCTC 
TATAGCAGCC TGTATGACCT TCGTGGTTCT ATTACTCCAC CCTCACCTGC CAATCCCCTT 
GGGGACCCCT ACTTTTCCCC CTCCCCCCCT CTATTTGCCC CCTCGGGTGC TCCCCCTCCT 
CCAAATTCCT CTCTGGTGGT CTCACGGTCC CTGTCACCCA CTCACTTCCT GTCAGGGACT 
TCATCCCCAC CTCTCCACCC CATTCCTCCA CCCACTTGCC TGAGTTACCC CCATCTTCCA 
CCCCCCTCAC CCACAAAGCC CTTTGCCTCA AAACCTCTCC CATACTGGAC CAAGTATGAT 
GTTGCAGATT GGCTGGGCTA CCTGAACCTG GGTGAACACA GGGAGCGATT CTTAGACAAT 
GAAATTGATG GCACGCACCT GCCCTCTCTT ACCAAGGATG ACTACCTGGA CCTGGGTGTG 
ACCCGAGTTG GCCATCGCAT GAACATAGAG AGGGCGCTCA GGAGAGTTAT GGACAGGCTG 
TCTTCTAGCA CTTTCCCCAT TTCTGCTCTC TCTCCTCGAA ATGGACGGAC AAACAGAAGG 
AGAGATGAGG CCAACCGGAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329737.1
CDS	381..7784
Translation	MTMPLSPLSSDEEQQRMLGNSQHLYPGAEEEEEEEEEMDDDGMDEEGEEENGELEMDDEEEVDDEDDIRRGPGHLRGGREEGHRQKGTMAGRLPGDRPIRPGNPGHAANPYSSNPGPTHQTSTNQQQQQPQRKLRERSSSNAPDDTHVALMVFRIGIPDIKQTKCLRFNPDATVWKAKQQVMCSLTESLRDVLNYGLFQPATDGHDAKFLEEERPLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKASLKKFMDYIQTSAVEKVAKLIDKGLDPNYHDPDTGETPLTLAVQTEPGGSEIIRVLVMGGAHIDFRARDGLTPLHKAVRAHAHTALLTLLSLGASSDYKDSRGLTPLYHTVLIGGDTSCCETLLYHRARLGLRDENGWDETHQACQNGNSQHLEHLLFYGADSSSQNASGNTALHICALYNKESCARILLYRGAKKDTKNNSGQNSFQVAVMSGHFELGEIIKNHRESDVVPFLESPKYAPQRRESSRTLGLPHPHPFLRANSDNSMNVPDWIAFPNAAGSSIVSVQGLKHGGTLRSSSSPRGARTRSPSRGRTGDRDDRSRQTRGRQGAGSISSQGTASGQRRRLYSAVPGRVFVATRSHSAQGDREISFNKGDKVKVLSVGEGGYWEGTVRGRTGWFPSDCVEEVALRSLESRSESRSERAKRLFRHYTVGSYDSFDAPSDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMVDRASAPWKKKSEFESSQATEKKRTVYQMALNKLDEILAAAQQTINTNEAPGPRVQGVKRERGRGFYNEPNFDQTGMGMTSSGAGLGYDRGQFVSGHGPPHGMLRQKSIGVPEEEKQFLHPPAMKFARSLSVPGPDEIPPPPTTAPPEPPFSSAPPLGFRGKTSQQPSVSMSQSTSSAAHYQLYSQSVHVTHGPRDRSPGPPTGSGTGDLAYSHAQAHPSVPSRTASRKVIGYTGDLAGAGGVAGAKAAALRRGYSNAVPPTSVATVIPQQQQTTQNQPSRMDRSGMGVGAGGVPKGGARRGKGPLVKQSKVEDLRQGQDTLEAKVSVEKSSIPIPTIIVKAPSTSSSGRSSQGSSVEAEPPPPGESDSSKPEPVVPPPAPTVPPPAPPSIPAPPPPSLPSLRTQESLDFTSQFGAAIVGAARRDRERFHEARRKSASFFLSAEEELSGAGGAGGRTQTSQQQLSSQPSPRLRPSKSIDEGMFSGDTFIHHTRSMPPAFGLPEYSSPAPQGDYQPKSVPADFYGAGSRQQQQQQQQQAPTTTFIHPLTGKVLDPSSPLGLALAARERALKDDGRIRRERTTEHHFTRQLSSIGAFSSSTSQPTSHISYPMASSSSATSSAAQSTNTSSYLVTSSSLAATTTSTRPPSPRILRGAGSSWSEEAGVGERADREAAGTSAPREGLRVRFSEDKTVHTHHYQSQQYQPTFKERERERQRERSREREREREREREIEKAQTQYQQQSSQPMTQQPRRPSFLRMESEAGAYILSLTPPSPPPTAAPMRPTAGATGESGSGTSLMVLPPPAPSVDADDEFVFADPLPPPLEFANSFDRGLGYSAMISHNVAARQQRHPPPPPPPPPPPTSKDSFMTGFPAHTPLPSPQAGDSTTSSLTSYDSEVANLTQSAPSPSPSSPNPLPPHSPSTLQTSGPPPPPSVSPPPPPNSYHRPFGLSQSQHLYNSTHTTGRSSSPTPPLSIPAPPAYRGPPTASSTPATSVSIRGASTVTASCSTTASTTVTSPSTTAAATPHTRIATVSTVATGGSRRPSAEHHPPPSATKSGESQETVVDSGIEELDSRSSSDHHLDSILALSGSGVRERLERAGMSSIGGIGGRDGATEVDRTSGADFLESYMSYLDGQTFEMHNAKAASTASTYPKTQRYREGTRAGDLRRQTSTAPPAYHRRREEEEEEDEAEEVEEEDLSARDGYGMREGPGIARSSVMYFDRPRTPEIKPLWGDGSTGASQTMGEGGGQIVGTYVEPRKLHPPMTGMKANIINELSTKLQQRSKGTENWGAQRSLSRHRFSEDSTGALSPPSVRSSSPSPVQLSQPALSPSPTPSSQPLYSNWARSPSPQPPAASSQPPPRSHSPVSPSSYSPYPTSPKHRPVYRTKALEFQFIPSRESRKSESHHAQRRRAPSPLISPSDRPKLGPPRPSSLPILPTTPLYSSLYDLRGSITPPSPANPLGDPYFSPSPPLFAPSGAPPPPNSSLVVSRSLSPTHFLSGTSSPPLHPIPPPTCLSYPHLPPPSPTKPFASKPLPYWTKYDVADWLGYLNLGEHRERFLDNEIDGTHLPSLTKDDYLDLGVTRVGHRMNIERALRRVMDRLSSSTFPISALSPRNGRTNRRRDEANRS

Target ORF information:

RefSeq Version	XM_021474062.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474062.1

ORF Insert Sequence:

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGTG 
GACAGAGCCT CTGCCCCATG GAAAAAAAAA TCAGAGTTCG AATCTTCACA AGCTACAGAG 
AAGAAGAGGA CAGTTTATCA GATGGCTTTG AATAAACTGG ATGAGATTTT AGCAGCAGCA 
CAGCAGACCA TTAATACAAA TGAGGCTCCA GGGCCCCGGG TTCAGGGGGT AAAAAGAGAA 
CGGGGCAGAG GCTTTTATAA TGAGCCAAAT TTCGACCAAA CTGGAATGGG GATGACATCA 
TCTGGGGCAG GGTTAGGCTA CGACCGTGGT CAATTTGTGT CTGGCCATGG CCCCCCGCAC 
GGTATGCTGC GACAGAAGTC TATCGGGGTT CCTGAGGAGG AGAAGCAGTT TCTTCATCCT 
CCAGCCATGA AGTTTGCCCG TAGTCTTTCA GTACCTGGCC CAGATGAAAT CCCTCCTCCA 
CCTACTACAG CACCCCCTGA GCCCCCTTTT TCCTCAGCTC CTCCGCTTGG CTTTAGGGGC 
AAGACATCTC AACAGCCGTC TGTTTCTATG TCTCAGTCTA CTTCCTCTGC TGCACATTAT 
CAACTCTACT CACAGTCAGT ACATGTTACT CACGGACCAA GGGACAGGTC CCCTGGACCA 
CCTACTGGGT CTGGAACTGG GGACCTTGCT TATTCCCATG CCCAAGCCCA CCCCTCTGTA 
CCAAGTAGGA CTGCCTCCAG GAAGGTGATT GGGTACACAG GAGATCTTGC AGGGGCTGGA 
GGAGTGGCTG GGGCCAAAGC TGCAGCATTG AGAAGGGGTT ACAGCAATGC AGTCCCACCC 
ACCAGTGTCG CTACTGTGAT TCCACAACAG CAGCAGACTA CTCAGAACCA ACCTTCCAGA 
ATGGACCGGA GTGGAATGGG GGTAGGGGCT GGTGGTGTAC CAAAAGGCGG TGCTCGTAGA 
GGAAAGGGGC CCTTGGTGAA ACAGTCAAAG GTGGAAGACC TGCGTCAAGG TCAAGACACT 
CTAGAAGCAA AGGTGTCAGT GGAGAAGAGC TCTATTCCAA TCCCAACTAT CATTGTTAAA 
GCCCCTTCCA CCAGCAGTAG TGGACGCAGT AGCCAAGGCA GCAGTGTGGA GGCTGAACCA 
CCGCCCCCAG GAGAAAGTGA CAGTTCTAAA CCAGAACCTG TTGTTCCCCC ACCTGCACCA 
ACCGTGCCAC CACCAGCCCC TCCATCTATA CCTGCTCCTC CACCCCCATC ACTGCCATCA 
CTTCGTACTC AAGAGAGTCT GGACTTCACC AGCCAGTTTG GTGCTGCCAT TGTAGGGGCA 
GCTCGCAGGG ACAGAGAACG ATTTCATGAA GCACGACGCA AGAGTGCCTC CTTTTTTCTT 
TCTGCTGAGG AAGAGTTGAG TGGAGCTGGA GGAGCAGGAG GGCGGACTCA GACTTCTCAA 
CAGCAGCTGA GTTCCCAGCC TTCACCACGC CTGCGACCAT CCAAATCTAT AGATGAAGGC 
ATGTTCTCAG GTGATACTTT TATCCATCAC ACACGCAGTA TGCCTCCTGC ATTTGGCTTG 
CCTGAGTACT CCTCACCTGC TCCTCAAGGG GATTACCAGC CTAAGTCTGT ACCTGCTGAC 
TTCTATGGGG CAGGATCCAG ACAACAGCAG CAGCAGCAGC AGCAGCAAGC CCCCACCACA 
ACTTTTATTC ACCCACTAAC TGGAAAAGTA CTGGACCCTT CATCCCCTCT AGGCTTGGCT 
TTGGCTGCAC GGGAACGTGC CTTGAAGGAT GATGGACGTA TACGTAGGGA AAGGACCACT 
GAACATCATT TTACCCGTCA GTTGTCAAGC ATTGGGGCCT TTTCCTCATC CACTTCACAG 
CCAACATCAC ACATTTCCTA CCCAATGGCC TCGTCTTCCT CTGCAACCTC CTCTGCTGCT 
CAATCCACAA ACACCTCTTC ATATCTTGTT ACCTCCTCAT CATTAGCTGC CACCACCACA 
TCCACTCGGC CTCCATCTCC TAGGATCCTG CGTGGAGCAG GGAGTAGCTG GAGTGAAGAA 
GCTGGGGTTG GAGAGCGTGC GGATAGGGAA GCAGCTGGCA CCTCTGCTCC CAGAGAAGGC 
CTAAGGGTTC GTTTCTCCGA GGATAAAACT GTCCACACAC ACCACTATCA ATCTCAGCAA 
TATCAGCCCA CCTTTAAAGA GCGTGAAAGA GAACGTCAAA GAGAGAGGTC AAGAGAAAGA 
GAGAGGGAAA GAGAGAGGGA AAGAGAAATA GAAAAAGCTC AAACCCAGTA TCAGCAACAG 
TCATCACAAC CAATGACCCA GCAGCCTCGA CGCCCCTCAT TCTTGAGGAT GGAGAGTGAA 
GCTGGAGCGT ACATTCTTTC ATTAACGCCT CCATCTCCTC CTCCAACAGC AGCCCCTATG 
CGTCCTACAG CAGGAGCCAC AGGTGAAAGT GGAAGTGGTA CAAGCCTGAT GGTTCTGCCT 
CCCCCAGCTC CCTCAGTAGA TGCAGATGAT GAGTTTGTGT TTGCTGATCC TCTTCCTCCA 
CCTCTAGAGT TTGCCAATAG TTTTGATCGT GGTTTGGGTT ACTCTGCGAT GATTTCTCAC 
AATGTTGCAG CCCGTCAGCA GCGTCATCCT CCACCACCCC CTCCCCCACC ACCCCCGCCC 
ACTTCGAAAG ATTCCTTTAT GACAGGCTTT CCAGCTCACA CACCCCTACC GTCACCTCAG 
GCAGGAGATT CCACAACTTC CAGTCTTACC TCCTATGACA GTGAGGTTGC AAACCTAACA 
CAATCTGCCC CATCGCCCTC CCCATCTTCT CCCAACCCAC TACCACCTCA CTCCCCCTCC 
ACCCTTCAGA CATCTGGACC ACCTCCACCT CCCTCTGTAT CACCTCCTCC ACCACCCAAC 
AGCTACCATA GACCTTTTGG CTTATCTCAG TCACAACATT TATACAACAG TACACACACA 
ACTGGACGCT CCTCCTCTCC CACCCCACCT CTCTCTATTC CAGCTCCACC TGCCTACCGG 
GGGCCACCAA CAGCATCATC CACCCCTGCA ACTTCTGTAT CAATCAGAGG TGCCTCAACT 
GTGACCGCAA GCTGTTCCAC AACTGCTTCT ACAACTGTCA CCTCTCCTAG TACTACTGCT 
GCTGCCACAC CACACACACG CATAGCAACT GTCTCCACAG TAGCTACAGG GGGTAGTAGG 
CGTCCCAGTG CAGAGCATCA TCCACCACCT AGTGCAACCA AGAGTGGTGA GTCCCAGGAG 
ACAGTGGTAG ATTCTGGGAT TGAGGAGCTG GACAGCCGAA GCAGCAGTGA TCATCACTTA 
GACAGCATTC TTGCACTCAG TGGTAGTGGG GTCAGAGAGA GGTTGGAACG AGCAGGGATG 
AGTAGCATAG GTGGAATTGG AGGGAGAGAT GGTGCAACTG AGGTTGACAG GACAAGTGGG 
GCAGACTTTT TAGAGTCTTA CATGAGCTAC CTTGATGGGC AGACTTTTGA AATGCACAAT 
GCCAAAGCCG CTAGCACTGC ATCCACCTAC CCAAAAACAC AGAGGTACAG AGAAGGCACC 
CGGGCTGGAG ATCTTCGACG CCAGACCAGC ACAGCACCTC CTGCATACCA CCGTCGTAGA 
GAGGAGGAAG AGGAAGAGGA TGAGGCCGAG GAGGTAGAGG AGGAGGATTT GAGTGCCCGT 
GATGGCTATG GGATGAGGGA AGGGCCAGGG ATTGCTCGCT CCTCTGTAAT GTACTTTGAC 
CGGCCCCGCA CTCCAGAGAT AAAACCACTG TGGGGAGATG GGTCGACTGG AGCATCACAG 
ACCATGGGGG AGGGAGGGGG CCAGATAGTG GGAACATATG TGGAGCCACG TAAACTACAT 
CCTCCCATGA CTGGTATGAA AGCAAACATC ATTAATGAAC TAAGCACCAA ATTACAGCAG 
AGAAGTAAAG GAACAGAGAA CTGGGGGGCC CAGAGATCAT TGAGCAGACA TAGGTTCTCA 
GAGGACTCAA CAGGTGCACT GAGTCCTCCC TCTGTCCGCT CTAGCTCCCC ATCTCCAGTT 
CAGCTCTCTC AGCCTGCCTT GTCTCCCTCT CCCACTCCTT CCTCCCAGCC ACTTTACTCA 
AACTGGGCTC GATCTCCTTC CCCTCAGCCC CCTGCTGCCT CATCCCAGCC CCCTCCCCGA 
TCCCATTCAC CTGTTTCCCC TTCATCATAT TCACCATACC CCACCTCACC CAAACACCGG 
CCTGTTTATC GCACCAAAGC TCTGGAGTTC CAATTTATTC CCAGTCGAGA GTCACGAAAG 
TCAGAGTCGC ATCATGCCCA GCGCAGACGA GCACCCAGCC CTTTAATTTC TCCATCTGAC 
CGTCCCAAGC TTGGCCCTCC CCGCCCCTCT TCCCTTCCTA TTCTCCCCAC TACACCCCTC 
TATAGCAGCC TGTATGACCT TCGTGGTTCT ATTACTCCAC CCTCACCTGC CAATCCCCTT 
GGGGACCCCT ACTTTTCCCC CTCCCCCCCT CTATTTGCCC CCTCGGGTGC TCCCCCTCCT 
CCAAATTCCT CTCTGGTGGT CTCACGGTCC CTGTCACCCA CTCACTTCCT GTCAGGGACT 
TCATCCCCAC CTCTCCACCC CATTCCTCCA CCCACTTGCC TGAGTTACCC CCATCTTCCA 
CCCCCCTCAC CCACAAAGCC CTTTGCCTCA AAACCTCTCC CATACTGGAC CAAGTATGAT 
GTTGCAGATT GGCTGGGCTA CCTGAACCTG GGTGAACACA GGGAGCGATT CTTAGACAAT 
GAAATTGATG GCACGCACCT GCCCTCTCTT ACCAAGGATG ACTACCTGGA CCTGGGTGTG 
ACCCGAGTTG GCCATCGCAT GAACATAGAG AGGGCGCTCA GGAGAGTTAT GGACAGGCTG 
TCTTCTAGCA CTTTCCCCAT TTCTGCTCTC TCTCCTCGAA ATGGACGGAC AAACAGAAGG 
AGAGATGAGG CCAACCGGAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_021471434.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327109.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X4, mRNA.

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CloneID	ODa141766
Clone ID Related Accession (Same CDS sequence)	XM_021471434.1 , XM_021474073.1
Accession Version	XM_021471434.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394533.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGTG 
GACAGAGCCT CTGCCCCATG GAAAAAAAAA TCAGAGTTCG AATCTTCACA AGCTACAGAG 
AAGAAGAGGA CAGTTTATCA GATGGCTTTG AATAAACTGG ATGAGATTTT AGCAGCAGCA 
CAGCAGACCA TTAATACAAA TGAGGCTCCA GGGCCCCGGG TTCAGGGGGT AAAAAGAGAA 
CGGGGCAGAG GCTTTTATAA TGAGCCAAAT TTCGACCAAA CTGGAATGGG GATGACATCA 
TCTGGGGCAG GGTTAGGCTA CGACCGTGGT CAATTTGTGT CTGGCCATGG CCCCCCGCAC 
GGGGTTCCTG AGGAGGAGAA GCAGTTTCTT CATCCTCCAG CCATGAAGTT TGCCCGTAGT 
CTTTCAGTAC CTGGCCCAGA TGAAATCCCT CCTCCACCTA CTACAGCACC CCCTGAGCCC 
CCTTTTTCCT CAGCTCCTCC GCTTGGCTTT AGGGGCAAGA CATCTCAACA GCCGTCTGTT 
TCTATGTCTC AGTCTACTTC CTCTGCTGCA CATTATCAAC TCTACTCACA GTCAGTACAT 
GTTACTCACG GACCAAGGGA CAGGTCCCCT GGACCACCTA CTGGGTCTGG AACTGGGGAC 
CTTGCTTATT CCCATGCCCA AGCCCACCCC TCTGTACCAA GTAGGACTGC CTCCAGGAAG 
GTGATTGGGT ACACAGGAGA TCTTGCAGGG GCTGGAGGAG TGGCTGGGGC CAAAGCTGCA 
GCATTGAGAA GGGGTTACAG CAATGCAGTC CCACCCACCA GTGTCGCTAC TGTGATTCCA 
CAACAGCAGC AGACTACTCA GAACCAACCT TCCAGAATGG ACCGGAGTGG AATGGGGGTA 
GGGGCTGGTG GTGTACCAAA AGGCGGTGCT CGTAGAGGAA AGGGGCCCTT GGTGAAACAG 
TCAAAGGTGG AAGACCTGCG TCAAGGTCAA GACACTCTAG AAGCAAAGGT GTCAGTGGAG 
AAGAGCTCTA TTCCAATCCC AACTATCATT GTTAAAGCCC CTTCCACCAG CAGTAGTGGA 
CGCAGTAGCC AAGGCAGCAG TGTGGAGGCT GAACCACCGC CCCCAGGAGA AAGTGACAGT 
TCTAAACCAG AACCTGTTGT TCCCCCACCT GCACCAACCG TGCCACCACC AGCCCCTCCA 
TCTATACCTG CTCCTCCACC CCCATCACTG CCATCACTTC GTACTCAAGA GAGTCTGGAC 
TTCACCAGCC AGTTTGGTGC TGCCATTGTA GGGGCAGCTC GCAGGGACAG AGAACGATTT 
CATGAAGCAC GACGCAAGAG TGCCTCCTTT TTTCTTTCTG CTGAGGAAGA GTTGAGTGGA 
GCTGGAGGAG CAGGAGGGCG GACTCAGACT TCTCAACAGC AGCTGAGTTC CCAGCCTTCA 
CCACGCCTGC GACCATCCAA ATCTATAGAT GAAGGCATGT TCTCAGGTGA TACTTTTATC 
CATCACACAC GCAGTATGCC TCCTGCATTT GGCTTGCCTG AGTACTCCTC ACCTGCTCCT 
CAAGGGGATT ACCAGCCTAA GTCTGTACCT GCTGACTTCT ATGGGGCAGG ATCCAGACAA 
CAGCAGCAGC AGCAGCAGCA GCAAGCCCCC ACCACAACTT TTATTCACCC ACTAACTGGA 
AAAGTACTGG ACCCTTCATC CCCTCTAGGC TTGGCTTTGG CTGCACGGGA ACGTGCCTTG 
AAGGATGATG GACGTATACG TAGGGAAAGG ACCACTGAAC ATCATTTTAC CCGTCAGTTG 
TCAAGCATTG GGGCCTTTTC CTCATCCACT TCACAGCCAA CATCACACAT TTCCTACCCA 
ATGGCCTCGT CTTCCTCTGC AACCTCCTCT GCTGCTCAAT CCACAAACAC CTCTTCATAT 
CTTGTTACCT CCTCATCATT AGCTGCCACC ACCACATCCA CTCGGCCTCC ATCTCCTAGG 
ATCCTGCGTG GAGCAGGGAG TAGCTGGAGT GAAGAAGCTG GGGTTGGAGA GCGTGCGGAT 
AGGGAAGCAG CTGGCACCTC TGCTCCCAGA GAAGGCCTAA GGGTTCGTTT CTCCGAGGAT 
AAAACTGTCC ACACACACCA CTATCAATCT CAGCAATATC AGCCCACCTT TAAAGAGCGT 
GAAAGAGAAC GTCAAAGAGA GAGGTCAAGA GAAAGAGAGA GGGAAAGAGA GAGGGAAAGA 
GAAATAGAAA AAGCTCAAAC CCAGTATCAG CAACAGTCAT CACAACCAAT GACCCAGCAG 
CCTCGACGCC CCTCATTCTT GAGGATGGAG AGTGAAGCTG GAGCGTACAT TCTTTCATTA 
ACGCCTCCAT CTCCTCCTCC AACAGCAGCC CCTATGCGTC CTACAGCAGG AGCCACAGGT 
GAAAGTGGAA GTGGTACAAG CCTGATGGTT CTGCCTCCCC CAGCTCCCTC AGTAGATGCA 
GATGATGAGT TTGTGTTTGC TGATCCTCTT CCTCCACCTC TAGAGTTTGC CAATAGTTTT 
GATCGTGGTT TGGGTTACTC TGCGATGATT TCTCACAATG TTGCAGCCCG TCAGCAGCGT 
CATCCTCCAC CACCCCCTCC CCCACCACCC CCGCCCACTT CGAAAGATTC CTTTATGACA 
GGCTTTCCAG CTCACACACC CCTACCGTCA CCTCAGGCAG GAGATTCCAC AACTTCCAGT 
CTTACCTCCT ATGACAGTGA GGTTGCAAAC CTAACACAAT CTGCCCCATC GCCCTCCCCA 
TCTTCTCCCA ACCCACTACC ACCTCACTCC CCCTCCACCC TTCAGACATC TGGACCACCT 
CCACCTCCCT CTGTATCACC TCCTCCACCA CCCAACAGCT ACCATAGACC TTTTGGCTTA 
TCTCAGTCAC AACATTTATA CAACAGTACA CACACAACTG GACGCTCCTC CTCTCCCACC 
CCACCTCTCT CTATTCCAGC TCCACCTGCC TACCGGGGGC CACCAACAGC ATCATCCACC 
CCTGCAACTT CTGTATCAAT CAGAGGTGCC TCAACTGTGA CCGCAAGCTG TTCCACAACT 
GCTTCTACAA CTGTCACCTC TCCTAGTACT ACTGCTGCTG CCACACCACA CACACGCATA 
GCAACTGTCT CCACAGTAGC TACAGGGGGT AGTAGGCGTC CCAGTGCAGA GCATCATCCA 
CCACCTAGTG CAACCAAGAG TGGTGAGTCC CAGGAGACAG TGGTAGATTC TGGGATTGAG 
GAGCTGGACA GCCGAAGCAG CAGTGATCAT CACTTAGACA GCATTCTTGC ACTCAGTGGT 
AGTGGGGTCA GAGAGAGGTT GGAACGAGCA GGGATGAGTA GCATAGGTGG AATTGGAGGG 
AGAGATGGTG CAACTGAGGT TGACAGGACA AGTGGGGCAG ACTTTTTAGA GTCTTACATG 
AGCTACCTTG ATGGGCAGAC TTTTGAAATG CACAATGCCA AAGCCGCTAG CACTGCATCC 
ACCTACCCAA AAACACAGAG GTACAGAGAA GGCACCCGGG CTGGAGATCT TCGACGCCAG 
ACCAGCACAG CACCTCCTGC ATACCACCGT CGTAGAGAGG AGGAAGAGGA AGAGGATGAG 
GCCGAGGAGG TAGAGGAGGA GGATTTGAGT GCCCGTGATG GCTATGGGAT GAGGGAAGGG 
CCAGGGATTG CTCGCTCCTC TGTAATGTAC TTTGACCGGC CCCGCACTCC AGAGATAAAA 
CCACTGTGGG GAGATGGGTC GACTGGAGCA TCACAGACCA TGGGGGAGGG AGGGGGCCAG 
ATAGTGGGAA CATATGTGGA GCCACGTAAA CTACATCCTC CCATGACTGG TATGAAAGCA 
AACATCATTA ATGAACTAAG CACCAAATTA CAGCAGAGAA GTAAAGGAAC AGAGAACTGG 
GGGGCCCAGA GATCATTGAG CAGACATAGA CACAGGTTCT CAGAGGACTC AACAGGTGCA 
CTGAGTCCTC CCTCTGTCCG CTCTAGCTCC CCATCTCCAG TTCAGCTCTC TCAGCCTGCC 
TTGTCTCCCT CTCCCACTCC TTCCTCCCAG CCACTTTACT CAAACTGGGC TCGATCTCCT 
TCCCCTCAGC CCCCTGCTGC CTCATCCCAG CCCCCTCCCC GATCCCATTC ACCTGTTTCC 
CCTTCATCAT ATTCACCATA CCCCACCTCA CCCAAACACC GGCCTGTTTA TCGCACCAAA 
GCTCTGGAGT TCCAATTTAT TCCCAGTCGA GAGTCACGAA AGTCAGAGTC GCATCATGCC 
CAGCGCAGAC GAGCACCCAG CCCTTTAATT TCTCCATCTG ACCGTCCCAA GCTTGGCCCT 
CCCCGCCCCT CTTCCCTTCC TATTCTCCCC ACTACACCCC TCTATAGCAG CCTGTATGAC 
CTTCGTGGTT CTATTACTCC ACCCTCACCT GCCAATCCCC TTGGGGACCC CTACTTTTCC 
CCCTCCCCCC CTCTATTTGC CCCCTCGGGT GCTCCCCCTC CTCCAAATTC CTCTCTGGTG 
GTCTCACGGT CCCTGTCACC CACTCACTTC CTGTCAGGGA CTTCATCCCC ACCTCTCCAC 
CCCATTCCTC CACCCACTTG CCTGAGTTAC CCCCATCTTC CACCCCCCTC ACCCACAAAG 
CCCTTTGCCT CAAAACCTCT CCCATACTGG ACCAAGTATG ATGTTGCAGA TTGGCTGGGC 
TACCTGAACC TGGGTGAACA CAGGGAGCGA TTCTTAGACA ATGAAATTGA TGGCACGCAC 
CTGCCCTCTC TTACCAAGGA TGACTACCTG GACCTGGGTG TGACCCGAGT TGGCCATCGC 
ATGAACATAG AGAGGGCGCT CAGGAGAGTT ATGGACAGGC TGTCTTCTAG CACTTTCCCC 
ATTTCTGCTC TCTCTCCTCG AAATGGACGG ACAAACAGAA GGAGAGATGA GGCCAACCGG 
AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327109.1
CDS	380..7765
Translation	MTMPLSPLSSDEEQQRMLGNSQHLYPGAEEEEEEEEEMDDDGMDEEGEEENGELEMDDEEEVDDEDDIRRGPGHLRGGREEGHRQKGTMAGRLPGDRPIRPGNPGHAANPYSSNPGPTHQTSTNQQQQQPQRKLRERSSSNAPDDTHVALMVFRIGIPDIKQTKCLRFNPDATVWKAKQQVMCSLTESLRDVLNYGLFQPATDGHDAKFLEEERPLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKASLKKFMDYIQTSAVEKVAKLIDKGLDPNYHDPDTGETPLTLAVQTEPGGSEIIRVLVMGGAHIDFRARDGLTPLHKAVRAHAHTALLTLLSLGASSDYKDSRGLTPLYHTVLIGGDTSCCETLLYHRARLGLRDENGWDETHQACQNGNSQHLEHLLFYGADSSSQNASGNTALHICALYNKESCARILLYRGAKKDTKNNSGQNSFQVAVMSGHFELGEIIKNHRESDVVPFLESPKYAPQRRESSRTLGLPHPHPFLRANSDNSMNVPDWIAFPNAAGSSIVSVQGLKHGGTLRSSSSPRGARTRSPSRGRTGDRDDRSRQTRGRQGAGSISSQGTASGQRRRLYSAVPGRVFVATRSHSAQGDREISFNKGDKVKVLSVGEGGYWEGTVRGRTGWFPSDCVEEVALRSLESRSESRSERAKRLFRHYTVGSYDSFDAPSDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMVDRASAPWKKKSEFESSQATEKKRTVYQMALNKLDEILAAAQQTINTNEAPGPRVQGVKRERGRGFYNEPNFDQTGMGMTSSGAGLGYDRGQFVSGHGPPHGVPEEEKQFLHPPAMKFARSLSVPGPDEIPPPPTTAPPEPPFSSAPPLGFRGKTSQQPSVSMSQSTSSAAHYQLYSQSVHVTHGPRDRSPGPPTGSGTGDLAYSHAQAHPSVPSRTASRKVIGYTGDLAGAGGVAGAKAAALRRGYSNAVPPTSVATVIPQQQQTTQNQPSRMDRSGMGVGAGGVPKGGARRGKGPLVKQSKVEDLRQGQDTLEAKVSVEKSSIPIPTIIVKAPSTSSSGRSSQGSSVEAEPPPPGESDSSKPEPVVPPPAPTVPPPAPPSIPAPPPPSLPSLRTQESLDFTSQFGAAIVGAARRDRERFHEARRKSASFFLSAEEELSGAGGAGGRTQTSQQQLSSQPSPRLRPSKSIDEGMFSGDTFIHHTRSMPPAFGLPEYSSPAPQGDYQPKSVPADFYGAGSRQQQQQQQQQAPTTTFIHPLTGKVLDPSSPLGLALAARERALKDDGRIRRERTTEHHFTRQLSSIGAFSSSTSQPTSHISYPMASSSSATSSAAQSTNTSSYLVTSSSLAATTTSTRPPSPRILRGAGSSWSEEAGVGERADREAAGTSAPREGLRVRFSEDKTVHTHHYQSQQYQPTFKERERERQRERSREREREREREREIEKAQTQYQQQSSQPMTQQPRRPSFLRMESEAGAYILSLTPPSPPPTAAPMRPTAGATGESGSGTSLMVLPPPAPSVDADDEFVFADPLPPPLEFANSFDRGLGYSAMISHNVAARQQRHPPPPPPPPPPPTSKDSFMTGFPAHTPLPSPQAGDSTTSSLTSYDSEVANLTQSAPSPSPSSPNPLPPHSPSTLQTSGPPPPPSVSPPPPPNSYHRPFGLSQSQHLYNSTHTTGRSSSPTPPLSIPAPPAYRGPPTASSTPATSVSIRGASTVTASCSTTASTTVTSPSTTAAATPHTRIATVSTVATGGSRRPSAEHHPPPSATKSGESQETVVDSGIEELDSRSSSDHHLDSILALSGSGVRERLERAGMSSIGGIGGRDGATEVDRTSGADFLESYMSYLDGQTFEMHNAKAASTASTYPKTQRYREGTRAGDLRRQTSTAPPAYHRRREEEEEEDEAEEVEEEDLSARDGYGMREGPGIARSSVMYFDRPRTPEIKPLWGDGSTGASQTMGEGGGQIVGTYVEPRKLHPPMTGMKANIINELSTKLQQRSKGTENWGAQRSLSRHRHRFSEDSTGALSPPSVRSSSPSPVQLSQPALSPSPTPSSQPLYSNWARSPSPQPPAASSQPPPRSHSPVSPSSYSPYPTSPKHRPVYRTKALEFQFIPSRESRKSESHHAQRRRAPSPLISPSDRPKLGPPRPSSLPILPTTPLYSSLYDLRGSITPPSPANPLGDPYFSPSPPLFAPSGAPPPPNSSLVVSRSLSPTHFLSGTSSPPLHPIPPPTCLSYPHLPPPSPTKPFASKPLPYWTKYDVADWLGYLNLGEHRERFLDNEIDGTHLPSLTKDDYLDLGVTRVGHRMNIERALRRVMDRLSSSTFPISALSPRNGRTNRRRDEANRS

Target ORF information:

RefSeq Version	XM_021471434.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471434.1

ORF Insert Sequence:

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGTG 
GACAGAGCCT CTGCCCCATG GAAAAAAAAA TCAGAGTTCG AATCTTCACA AGCTACAGAG 
AAGAAGAGGA CAGTTTATCA GATGGCTTTG AATAAACTGG ATGAGATTTT AGCAGCAGCA 
CAGCAGACCA TTAATACAAA TGAGGCTCCA GGGCCCCGGG TTCAGGGGGT AAAAAGAGAA 
CGGGGCAGAG GCTTTTATAA TGAGCCAAAT TTCGACCAAA CTGGAATGGG GATGACATCA 
TCTGGGGCAG GGTTAGGCTA CGACCGTGGT CAATTTGTGT CTGGCCATGG CCCCCCGCAC 
GGGGTTCCTG AGGAGGAGAA GCAGTTTCTT CATCCTCCAG CCATGAAGTT TGCCCGTAGT 
CTTTCAGTAC CTGGCCCAGA TGAAATCCCT CCTCCACCTA CTACAGCACC CCCTGAGCCC 
CCTTTTTCCT CAGCTCCTCC GCTTGGCTTT AGGGGCAAGA CATCTCAACA GCCGTCTGTT 
TCTATGTCTC AGTCTACTTC CTCTGCTGCA CATTATCAAC TCTACTCACA GTCAGTACAT 
GTTACTCACG GACCAAGGGA CAGGTCCCCT GGACCACCTA CTGGGTCTGG AACTGGGGAC 
CTTGCTTATT CCCATGCCCA AGCCCACCCC TCTGTACCAA GTAGGACTGC CTCCAGGAAG 
GTGATTGGGT ACACAGGAGA TCTTGCAGGG GCTGGAGGAG TGGCTGGGGC CAAAGCTGCA 
GCATTGAGAA GGGGTTACAG CAATGCAGTC CCACCCACCA GTGTCGCTAC TGTGATTCCA 
CAACAGCAGC AGACTACTCA GAACCAACCT TCCAGAATGG ACCGGAGTGG AATGGGGGTA 
GGGGCTGGTG GTGTACCAAA AGGCGGTGCT CGTAGAGGAA AGGGGCCCTT GGTGAAACAG 
TCAAAGGTGG AAGACCTGCG TCAAGGTCAA GACACTCTAG AAGCAAAGGT GTCAGTGGAG 
AAGAGCTCTA TTCCAATCCC AACTATCATT GTTAAAGCCC CTTCCACCAG CAGTAGTGGA 
CGCAGTAGCC AAGGCAGCAG TGTGGAGGCT GAACCACCGC CCCCAGGAGA AAGTGACAGT 
TCTAAACCAG AACCTGTTGT TCCCCCACCT GCACCAACCG TGCCACCACC AGCCCCTCCA 
TCTATACCTG CTCCTCCACC CCCATCACTG CCATCACTTC GTACTCAAGA GAGTCTGGAC 
TTCACCAGCC AGTTTGGTGC TGCCATTGTA GGGGCAGCTC GCAGGGACAG AGAACGATTT 
CATGAAGCAC GACGCAAGAG TGCCTCCTTT TTTCTTTCTG CTGAGGAAGA GTTGAGTGGA 
GCTGGAGGAG CAGGAGGGCG GACTCAGACT TCTCAACAGC AGCTGAGTTC CCAGCCTTCA 
CCACGCCTGC GACCATCCAA ATCTATAGAT GAAGGCATGT TCTCAGGTGA TACTTTTATC 
CATCACACAC GCAGTATGCC TCCTGCATTT GGCTTGCCTG AGTACTCCTC ACCTGCTCCT 
CAAGGGGATT ACCAGCCTAA GTCTGTACCT GCTGACTTCT ATGGGGCAGG ATCCAGACAA 
CAGCAGCAGC AGCAGCAGCA GCAAGCCCCC ACCACAACTT TTATTCACCC ACTAACTGGA 
AAAGTACTGG ACCCTTCATC CCCTCTAGGC TTGGCTTTGG CTGCACGGGA ACGTGCCTTG 
AAGGATGATG GACGTATACG TAGGGAAAGG ACCACTGAAC ATCATTTTAC CCGTCAGTTG 
TCAAGCATTG GGGCCTTTTC CTCATCCACT TCACAGCCAA CATCACACAT TTCCTACCCA 
ATGGCCTCGT CTTCCTCTGC AACCTCCTCT GCTGCTCAAT CCACAAACAC CTCTTCATAT 
CTTGTTACCT CCTCATCATT AGCTGCCACC ACCACATCCA CTCGGCCTCC ATCTCCTAGG 
ATCCTGCGTG GAGCAGGGAG TAGCTGGAGT GAAGAAGCTG GGGTTGGAGA GCGTGCGGAT 
AGGGAAGCAG CTGGCACCTC TGCTCCCAGA GAAGGCCTAA GGGTTCGTTT CTCCGAGGAT 
AAAACTGTCC ACACACACCA CTATCAATCT CAGCAATATC AGCCCACCTT TAAAGAGCGT 
GAAAGAGAAC GTCAAAGAGA GAGGTCAAGA GAAAGAGAGA GGGAAAGAGA GAGGGAAAGA 
GAAATAGAAA AAGCTCAAAC CCAGTATCAG CAACAGTCAT CACAACCAAT GACCCAGCAG 
CCTCGACGCC CCTCATTCTT GAGGATGGAG AGTGAAGCTG GAGCGTACAT TCTTTCATTA 
ACGCCTCCAT CTCCTCCTCC AACAGCAGCC CCTATGCGTC CTACAGCAGG AGCCACAGGT 
GAAAGTGGAA GTGGTACAAG CCTGATGGTT CTGCCTCCCC CAGCTCCCTC AGTAGATGCA 
GATGATGAGT TTGTGTTTGC TGATCCTCTT CCTCCACCTC TAGAGTTTGC CAATAGTTTT 
GATCGTGGTT TGGGTTACTC TGCGATGATT TCTCACAATG TTGCAGCCCG TCAGCAGCGT 
CATCCTCCAC CACCCCCTCC CCCACCACCC CCGCCCACTT CGAAAGATTC CTTTATGACA 
GGCTTTCCAG CTCACACACC CCTACCGTCA CCTCAGGCAG GAGATTCCAC AACTTCCAGT 
CTTACCTCCT ATGACAGTGA GGTTGCAAAC CTAACACAAT CTGCCCCATC GCCCTCCCCA 
TCTTCTCCCA ACCCACTACC ACCTCACTCC CCCTCCACCC TTCAGACATC TGGACCACCT 
CCACCTCCCT CTGTATCACC TCCTCCACCA CCCAACAGCT ACCATAGACC TTTTGGCTTA 
TCTCAGTCAC AACATTTATA CAACAGTACA CACACAACTG GACGCTCCTC CTCTCCCACC 
CCACCTCTCT CTATTCCAGC TCCACCTGCC TACCGGGGGC CACCAACAGC ATCATCCACC 
CCTGCAACTT CTGTATCAAT CAGAGGTGCC TCAACTGTGA CCGCAAGCTG TTCCACAACT 
GCTTCTACAA CTGTCACCTC TCCTAGTACT ACTGCTGCTG CCACACCACA CACACGCATA 
GCAACTGTCT CCACAGTAGC TACAGGGGGT AGTAGGCGTC CCAGTGCAGA GCATCATCCA 
CCACCTAGTG CAACCAAGAG TGGTGAGTCC CAGGAGACAG TGGTAGATTC TGGGATTGAG 
GAGCTGGACA GCCGAAGCAG CAGTGATCAT CACTTAGACA GCATTCTTGC ACTCAGTGGT 
AGTGGGGTCA GAGAGAGGTT GGAACGAGCA GGGATGAGTA GCATAGGTGG AATTGGAGGG 
AGAGATGGTG CAACTGAGGT TGACAGGACA AGTGGGGCAG ACTTTTTAGA GTCTTACATG 
AGCTACCTTG ATGGGCAGAC TTTTGAAATG CACAATGCCA AAGCCGCTAG CACTGCATCC 
ACCTACCCAA AAACACAGAG GTACAGAGAA GGCACCCGGG CTGGAGATCT TCGACGCCAG 
ACCAGCACAG CACCTCCTGC ATACCACCGT CGTAGAGAGG AGGAAGAGGA AGAGGATGAG 
GCCGAGGAGG TAGAGGAGGA GGATTTGAGT GCCCGTGATG GCTATGGGAT GAGGGAAGGG 
CCAGGGATTG CTCGCTCCTC TGTAATGTAC TTTGACCGGC CCCGCACTCC AGAGATAAAA 
CCACTGTGGG GAGATGGGTC GACTGGAGCA TCACAGACCA TGGGGGAGGG AGGGGGCCAG 
ATAGTGGGAA CATATGTGGA GCCACGTAAA CTACATCCTC CCATGACTGG TATGAAAGCA 
AACATCATTA ATGAACTAAG CACCAAATTA CAGCAGAGAA GTAAAGGAAC AGAGAACTGG 
GGGGCCCAGA GATCATTGAG CAGACATAGA CACAGGTTCT CAGAGGACTC AACAGGTGCA 
CTGAGTCCTC CCTCTGTCCG CTCTAGCTCC CCATCTCCAG TTCAGCTCTC TCAGCCTGCC 
TTGTCTCCCT CTCCCACTCC TTCCTCCCAG CCACTTTACT CAAACTGGGC TCGATCTCCT 
TCCCCTCAGC CCCCTGCTGC CTCATCCCAG CCCCCTCCCC GATCCCATTC ACCTGTTTCC 
CCTTCATCAT ATTCACCATA CCCCACCTCA CCCAAACACC GGCCTGTTTA TCGCACCAAA 
GCTCTGGAGT TCCAATTTAT TCCCAGTCGA GAGTCACGAA AGTCAGAGTC GCATCATGCC 
CAGCGCAGAC GAGCACCCAG CCCTTTAATT TCTCCATCTG ACCGTCCCAA GCTTGGCCCT 
CCCCGCCCCT CTTCCCTTCC TATTCTCCCC ACTACACCCC TCTATAGCAG CCTGTATGAC 
CTTCGTGGTT CTATTACTCC ACCCTCACCT GCCAATCCCC TTGGGGACCC CTACTTTTCC 
CCCTCCCCCC CTCTATTTGC CCCCTCGGGT GCTCCCCCTC CTCCAAATTC CTCTCTGGTG 
GTCTCACGGT CCCTGTCACC CACTCACTTC CTGTCAGGGA CTTCATCCCC ACCTCTCCAC 
CCCATTCCTC CACCCACTTG CCTGAGTTAC CCCCATCTTC CACCCCCCTC ACCCACAAAG 
CCCTTTGCCT CAAAACCTCT CCCATACTGG ACCAAGTATG ATGTTGCAGA TTGGCTGGGC 
TACCTGAACC TGGGTGAACA CAGGGAGCGA TTCTTAGACA ATGAAATTGA TGGCACGCAC 
CTGCCCTCTC TTACCAAGGA TGACTACCTG GACCTGGGTG TGACCCGAGT TGGCCATCGC 
ATGAACATAG AGAGGGCGCT CAGGAGAGTT ATGGACAGGC TGTCTTCTAG CACTTTCCCC 
ATTTCTGCTC TCTCTCCTCG AAATGGACGG ACAAACAGAA GGAGAGATGA GGCCAACCGG 
AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_021474073.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329748.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X4, mRNA.

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CloneID	ODa141766
Clone ID Related Accession (Same CDS sequence)	XM_021471434.1 , XM_021474073.1
Accession Version	XM_021474073.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGTG 
GACAGAGCCT CTGCCCCATG GAAAAAAAAA TCAGAGTTCG AATCTTCACA AGCTACAGAG 
AAGAAGAGGA CAGTTTATCA GATGGCTTTG AATAAACTGG ATGAGATTTT AGCAGCAGCA 
CAGCAGACCA TTAATACAAA TGAGGCTCCA GGGCCCCGGG TTCAGGGGGT AAAAAGAGAA 
CGGGGCAGAG GCTTTTATAA TGAGCCAAAT TTCGACCAAA CTGGAATGGG GATGACATCA 
TCTGGGGCAG GGTTAGGCTA CGACCGTGGT CAATTTGTGT CTGGCCATGG CCCCCCGCAC 
GGGGTTCCTG AGGAGGAGAA GCAGTTTCTT CATCCTCCAG CCATGAAGTT TGCCCGTAGT 
CTTTCAGTAC CTGGCCCAGA TGAAATCCCT CCTCCACCTA CTACAGCACC CCCTGAGCCC 
CCTTTTTCCT CAGCTCCTCC GCTTGGCTTT AGGGGCAAGA CATCTCAACA GCCGTCTGTT 
TCTATGTCTC AGTCTACTTC CTCTGCTGCA CATTATCAAC TCTACTCACA GTCAGTACAT 
GTTACTCACG GACCAAGGGA CAGGTCCCCT GGACCACCTA CTGGGTCTGG AACTGGGGAC 
CTTGCTTATT CCCATGCCCA AGCCCACCCC TCTGTACCAA GTAGGACTGC CTCCAGGAAG 
GTGATTGGGT ACACAGGAGA TCTTGCAGGG GCTGGAGGAG TGGCTGGGGC CAAAGCTGCA 
GCATTGAGAA GGGGTTACAG CAATGCAGTC CCACCCACCA GTGTCGCTAC TGTGATTCCA 
CAACAGCAGC AGACTACTCA GAACCAACCT TCCAGAATGG ACCGGAGTGG AATGGGGGTA 
GGGGCTGGTG GTGTACCAAA AGGCGGTGCT CGTAGAGGAA AGGGGCCCTT GGTGAAACAG 
TCAAAGGTGG AAGACCTGCG TCAAGGTCAA GACACTCTAG AAGCAAAGGT GTCAGTGGAG 
AAGAGCTCTA TTCCAATCCC AACTATCATT GTTAAAGCCC CTTCCACCAG CAGTAGTGGA 
CGCAGTAGCC AAGGCAGCAG TGTGGAGGCT GAACCACCGC CCCCAGGAGA AAGTGACAGT 
TCTAAACCAG AACCTGTTGT TCCCCCACCT GCACCAACCG TGCCACCACC AGCCCCTCCA 
TCTATACCTG CTCCTCCACC CCCATCACTG CCATCACTTC GTACTCAAGA GAGTCTGGAC 
TTCACCAGCC AGTTTGGTGC TGCCATTGTA GGGGCAGCTC GCAGGGACAG AGAACGATTT 
CATGAAGCAC GACGCAAGAG TGCCTCCTTT TTTCTTTCTG CTGAGGAAGA GTTGAGTGGA 
GCTGGAGGAG CAGGAGGGCG GACTCAGACT TCTCAACAGC AGCTGAGTTC CCAGCCTTCA 
CCACGCCTGC GACCATCCAA ATCTATAGAT GAAGGCATGT TCTCAGGTGA TACTTTTATC 
CATCACACAC GCAGTATGCC TCCTGCATTT GGCTTGCCTG AGTACTCCTC ACCTGCTCCT 
CAAGGGGATT ACCAGCCTAA GTCTGTACCT GCTGACTTCT ATGGGGCAGG ATCCAGACAA 
CAGCAGCAGC AGCAGCAGCA GCAAGCCCCC ACCACAACTT TTATTCACCC ACTAACTGGA 
AAAGTACTGG ACCCTTCATC CCCTCTAGGC TTGGCTTTGG CTGCACGGGA ACGTGCCTTG 
AAGGATGATG GACGTATACG TAGGGAAAGG ACCACTGAAC ATCATTTTAC CCGTCAGTTG 
TCAAGCATTG GGGCCTTTTC CTCATCCACT TCACAGCCAA CATCACACAT TTCCTACCCA 
ATGGCCTCGT CTTCCTCTGC AACCTCCTCT GCTGCTCAAT CCACAAACAC CTCTTCATAT 
CTTGTTACCT CCTCATCATT AGCTGCCACC ACCACATCCA CTCGGCCTCC ATCTCCTAGG 
ATCCTGCGTG GAGCAGGGAG TAGCTGGAGT GAAGAAGCTG GGGTTGGAGA GCGTGCGGAT 
AGGGAAGCAG CTGGCACCTC TGCTCCCAGA GAAGGCCTAA GGGTTCGTTT CTCCGAGGAT 
AAAACTGTCC ACACACACCA CTATCAATCT CAGCAATATC AGCCCACCTT TAAAGAGCGT 
GAAAGAGAAC GTCAAAGAGA GAGGTCAAGA GAAAGAGAGA GGGAAAGAGA GAGGGAAAGA 
GAAATAGAAA AAGCTCAAAC CCAGTATCAG CAACAGTCAT CACAACCAAT GACCCAGCAG 
CCTCGACGCC CCTCATTCTT GAGGATGGAG AGTGAAGCTG GAGCGTACAT TCTTTCATTA 
ACGCCTCCAT CTCCTCCTCC AACAGCAGCC CCTATGCGTC CTACAGCAGG AGCCACAGGT 
GAAAGTGGAA GTGGTACAAG CCTGATGGTT CTGCCTCCCC CAGCTCCCTC AGTAGATGCA 
GATGATGAGT TTGTGTTTGC TGATCCTCTT CCTCCACCTC TAGAGTTTGC CAATAGTTTT 
GATCGTGGTT TGGGTTACTC TGCGATGATT TCTCACAATG TTGCAGCCCG TCAGCAGCGT 
CATCCTCCAC CACCCCCTCC CCCACCACCC CCGCCCACTT CGAAAGATTC CTTTATGACA 
GGCTTTCCAG CTCACACACC CCTACCGTCA CCTCAGGCAG GAGATTCCAC AACTTCCAGT 
CTTACCTCCT ATGACAGTGA GGTTGCAAAC CTAACACAAT CTGCCCCATC GCCCTCCCCA 
TCTTCTCCCA ACCCACTACC ACCTCACTCC CCCTCCACCC TTCAGACATC TGGACCACCT 
CCACCTCCCT CTGTATCACC TCCTCCACCA CCCAACAGCT ACCATAGACC TTTTGGCTTA 
TCTCAGTCAC AACATTTATA CAACAGTACA CACACAACTG GACGCTCCTC CTCTCCCACC 
CCACCTCTCT CTATTCCAGC TCCACCTGCC TACCGGGGGC CACCAACAGC ATCATCCACC 
CCTGCAACTT CTGTATCAAT CAGAGGTGCC TCAACTGTGA CCGCAAGCTG TTCCACAACT 
GCTTCTACAA CTGTCACCTC TCCTAGTACT ACTGCTGCTG CCACACCACA CACACGCATA 
GCAACTGTCT CCACAGTAGC TACAGGGGGT AGTAGGCGTC CCAGTGCAGA GCATCATCCA 
CCACCTAGTG CAACCAAGAG TGGTGAGTCC CAGGAGACAG TGGTAGATTC TGGGATTGAG 
GAGCTGGACA GCCGAAGCAG CAGTGATCAT CACTTAGACA GCATTCTTGC ACTCAGTGGT 
AGTGGGGTCA GAGAGAGGTT GGAACGAGCA GGGATGAGTA GCATAGGTGG AATTGGAGGG 
AGAGATGGTG CAACTGAGGT TGACAGGACA AGTGGGGCAG ACTTTTTAGA GTCTTACATG 
AGCTACCTTG ATGGGCAGAC TTTTGAAATG CACAATGCCA AAGCCGCTAG CACTGCATCC 
ACCTACCCAA AAACACAGAG GTACAGAGAA GGCACCCGGG CTGGAGATCT TCGACGCCAG 
ACCAGCACAG CACCTCCTGC ATACCACCGT CGTAGAGAGG AGGAAGAGGA AGAGGATGAG 
GCCGAGGAGG TAGAGGAGGA GGATTTGAGT GCCCGTGATG GCTATGGGAT GAGGGAAGGG 
CCAGGGATTG CTCGCTCCTC TGTAATGTAC TTTGACCGGC CCCGCACTCC AGAGATAAAA 
CCACTGTGGG GAGATGGGTC GACTGGAGCA TCACAGACCA TGGGGGAGGG AGGGGGCCAG 
ATAGTGGGAA CATATGTGGA GCCACGTAAA CTACATCCTC CCATGACTGG TATGAAAGCA 
AACATCATTA ATGAACTAAG CACCAAATTA CAGCAGAGAA GTAAAGGAAC AGAGAACTGG 
GGGGCCCAGA GATCATTGAG CAGACATAGA CACAGGTTCT CAGAGGACTC AACAGGTGCA 
CTGAGTCCTC CCTCTGTCCG CTCTAGCTCC CCATCTCCAG TTCAGCTCTC TCAGCCTGCC 
TTGTCTCCCT CTCCCACTCC TTCCTCCCAG CCACTTTACT CAAACTGGGC TCGATCTCCT 
TCCCCTCAGC CCCCTGCTGC CTCATCCCAG CCCCCTCCCC GATCCCATTC ACCTGTTTCC 
CCTTCATCAT ATTCACCATA CCCCACCTCA CCCAAACACC GGCCTGTTTA TCGCACCAAA 
GCTCTGGAGT TCCAATTTAT TCCCAGTCGA GAGTCACGAA AGTCAGAGTC GCATCATGCC 
CAGCGCAGAC GAGCACCCAG CCCTTTAATT TCTCCATCTG ACCGTCCCAA GCTTGGCCCT 
CCCCGCCCCT CTTCCCTTCC TATTCTCCCC ACTACACCCC TCTATAGCAG CCTGTATGAC 
CTTCGTGGTT CTATTACTCC ACCCTCACCT GCCAATCCCC TTGGGGACCC CTACTTTTCC 
CCCTCCCCCC CTCTATTTGC CCCCTCGGGT GCTCCCCCTC CTCCAAATTC CTCTCTGGTG 
GTCTCACGGT CCCTGTCACC CACTCACTTC CTGTCAGGGA CTTCATCCCC ACCTCTCCAC 
CCCATTCCTC CACCCACTTG CCTGAGTTAC CCCCATCTTC CACCCCCCTC ACCCACAAAG 
CCCTTTGCCT CAAAACCTCT CCCATACTGG ACCAAGTATG ATGTTGCAGA TTGGCTGGGC 
TACCTGAACC TGGGTGAACA CAGGGAGCGA TTCTTAGACA ATGAAATTGA TGGCACGCAC 
CTGCCCTCTC TTACCAAGGA TGACTACCTG GACCTGGGTG TGACCCGAGT TGGCCATCGC 
ATGAACATAG AGAGGGCGCT CAGGAGAGTT ATGGACAGGC TGTCTTCTAG CACTTTCCCC 
ATTTCTGCTC TCTCTCCTCG AAATGGACGG ACAAACAGAA GGAGAGATGA GGCCAACCGG 
AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329748.1
CDS	380..7765
Translation	MTMPLSPLSSDEEQQRMLGNSQHLYPGAEEEEEEEEEMDDDGMDEEGEEENGELEMDDEEEVDDEDDIRRGPGHLRGGREEGHRQKGTMAGRLPGDRPIRPGNPGHAANPYSSNPGPTHQTSTNQQQQQPQRKLRERSSSNAPDDTHVALMVFRIGIPDIKQTKCLRFNPDATVWKAKQQVMCSLTESLRDVLNYGLFQPATDGHDAKFLEEERPLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKASLKKFMDYIQTSAVEKVAKLIDKGLDPNYHDPDTGETPLTLAVQTEPGGSEIIRVLVMGGAHIDFRARDGLTPLHKAVRAHAHTALLTLLSLGASSDYKDSRGLTPLYHTVLIGGDTSCCETLLYHRARLGLRDENGWDETHQACQNGNSQHLEHLLFYGADSSSQNASGNTALHICALYNKESCARILLYRGAKKDTKNNSGQNSFQVAVMSGHFELGEIIKNHRESDVVPFLESPKYAPQRRESSRTLGLPHPHPFLRANSDNSMNVPDWIAFPNAAGSSIVSVQGLKHGGTLRSSSSPRGARTRSPSRGRTGDRDDRSRQTRGRQGAGSISSQGTASGQRRRLYSAVPGRVFVATRSHSAQGDREISFNKGDKVKVLSVGEGGYWEGTVRGRTGWFPSDCVEEVALRSLESRSESRSERAKRLFRHYTVGSYDSFDAPSDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMVDRASAPWKKKSEFESSQATEKKRTVYQMALNKLDEILAAAQQTINTNEAPGPRVQGVKRERGRGFYNEPNFDQTGMGMTSSGAGLGYDRGQFVSGHGPPHGVPEEEKQFLHPPAMKFARSLSVPGPDEIPPPPTTAPPEPPFSSAPPLGFRGKTSQQPSVSMSQSTSSAAHYQLYSQSVHVTHGPRDRSPGPPTGSGTGDLAYSHAQAHPSVPSRTASRKVIGYTGDLAGAGGVAGAKAAALRRGYSNAVPPTSVATVIPQQQQTTQNQPSRMDRSGMGVGAGGVPKGGARRGKGPLVKQSKVEDLRQGQDTLEAKVSVEKSSIPIPTIIVKAPSTSSSGRSSQGSSVEAEPPPPGESDSSKPEPVVPPPAPTVPPPAPPSIPAPPPPSLPSLRTQESLDFTSQFGAAIVGAARRDRERFHEARRKSASFFLSAEEELSGAGGAGGRTQTSQQQLSSQPSPRLRPSKSIDEGMFSGDTFIHHTRSMPPAFGLPEYSSPAPQGDYQPKSVPADFYGAGSRQQQQQQQQQAPTTTFIHPLTGKVLDPSSPLGLALAARERALKDDGRIRRERTTEHHFTRQLSSIGAFSSSTSQPTSHISYPMASSSSATSSAAQSTNTSSYLVTSSSLAATTTSTRPPSPRILRGAGSSWSEEAGVGERADREAAGTSAPREGLRVRFSEDKTVHTHHYQSQQYQPTFKERERERQRERSREREREREREREIEKAQTQYQQQSSQPMTQQPRRPSFLRMESEAGAYILSLTPPSPPPTAAPMRPTAGATGESGSGTSLMVLPPPAPSVDADDEFVFADPLPPPLEFANSFDRGLGYSAMISHNVAARQQRHPPPPPPPPPPPTSKDSFMTGFPAHTPLPSPQAGDSTTSSLTSYDSEVANLTQSAPSPSPSSPNPLPPHSPSTLQTSGPPPPPSVSPPPPPNSYHRPFGLSQSQHLYNSTHTTGRSSSPTPPLSIPAPPAYRGPPTASSTPATSVSIRGASTVTASCSTTASTTVTSPSTTAAATPHTRIATVSTVATGGSRRPSAEHHPPPSATKSGESQETVVDSGIEELDSRSSSDHHLDSILALSGSGVRERLERAGMSSIGGIGGRDGATEVDRTSGADFLESYMSYLDGQTFEMHNAKAASTASTYPKTQRYREGTRAGDLRRQTSTAPPAYHRRREEEEEEDEAEEVEEEDLSARDGYGMREGPGIARSSVMYFDRPRTPEIKPLWGDGSTGASQTMGEGGGQIVGTYVEPRKLHPPMTGMKANIINELSTKLQQRSKGTENWGAQRSLSRHRHRFSEDSTGALSPPSVRSSSPSPVQLSQPALSPSPTPSSQPLYSNWARSPSPQPPAASSQPPPRSHSPVSPSSYSPYPTSPKHRPVYRTKALEFQFIPSRESRKSESHHAQRRRAPSPLISPSDRPKLGPPRPSSLPILPTTPLYSSLYDLRGSITPPSPANPLGDPYFSPSPPLFAPSGAPPPPNSSLVVSRSLSPTHFLSGTSSPPLHPIPPPTCLSYPHLPPPSPTKPFASKPLPYWTKYDVADWLGYLNLGEHRERFLDNEIDGTHLPSLTKDDYLDLGVTRVGHRMNIERALRRVMDRLSSSTFPISALSPRNGRTNRRRDEANRS

Target ORF information:

RefSeq Version	XM_021474073.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474073.1

ORF Insert Sequence:

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGTG 
GACAGAGCCT CTGCCCCATG GAAAAAAAAA TCAGAGTTCG AATCTTCACA AGCTACAGAG 
AAGAAGAGGA CAGTTTATCA GATGGCTTTG AATAAACTGG ATGAGATTTT AGCAGCAGCA 
CAGCAGACCA TTAATACAAA TGAGGCTCCA GGGCCCCGGG TTCAGGGGGT AAAAAGAGAA 
CGGGGCAGAG GCTTTTATAA TGAGCCAAAT TTCGACCAAA CTGGAATGGG GATGACATCA 
TCTGGGGCAG GGTTAGGCTA CGACCGTGGT CAATTTGTGT CTGGCCATGG CCCCCCGCAC 
GGGGTTCCTG AGGAGGAGAA GCAGTTTCTT CATCCTCCAG CCATGAAGTT TGCCCGTAGT 
CTTTCAGTAC CTGGCCCAGA TGAAATCCCT CCTCCACCTA CTACAGCACC CCCTGAGCCC 
CCTTTTTCCT CAGCTCCTCC GCTTGGCTTT AGGGGCAAGA CATCTCAACA GCCGTCTGTT 
TCTATGTCTC AGTCTACTTC CTCTGCTGCA CATTATCAAC TCTACTCACA GTCAGTACAT 
GTTACTCACG GACCAAGGGA CAGGTCCCCT GGACCACCTA CTGGGTCTGG AACTGGGGAC 
CTTGCTTATT CCCATGCCCA AGCCCACCCC TCTGTACCAA GTAGGACTGC CTCCAGGAAG 
GTGATTGGGT ACACAGGAGA TCTTGCAGGG GCTGGAGGAG TGGCTGGGGC CAAAGCTGCA 
GCATTGAGAA GGGGTTACAG CAATGCAGTC CCACCCACCA GTGTCGCTAC TGTGATTCCA 
CAACAGCAGC AGACTACTCA GAACCAACCT TCCAGAATGG ACCGGAGTGG AATGGGGGTA 
GGGGCTGGTG GTGTACCAAA AGGCGGTGCT CGTAGAGGAA AGGGGCCCTT GGTGAAACAG 
TCAAAGGTGG AAGACCTGCG TCAAGGTCAA GACACTCTAG AAGCAAAGGT GTCAGTGGAG 
AAGAGCTCTA TTCCAATCCC AACTATCATT GTTAAAGCCC CTTCCACCAG CAGTAGTGGA 
CGCAGTAGCC AAGGCAGCAG TGTGGAGGCT GAACCACCGC CCCCAGGAGA AAGTGACAGT 
TCTAAACCAG AACCTGTTGT TCCCCCACCT GCACCAACCG TGCCACCACC AGCCCCTCCA 
TCTATACCTG CTCCTCCACC CCCATCACTG CCATCACTTC GTACTCAAGA GAGTCTGGAC 
TTCACCAGCC AGTTTGGTGC TGCCATTGTA GGGGCAGCTC GCAGGGACAG AGAACGATTT 
CATGAAGCAC GACGCAAGAG TGCCTCCTTT TTTCTTTCTG CTGAGGAAGA GTTGAGTGGA 
GCTGGAGGAG CAGGAGGGCG GACTCAGACT TCTCAACAGC AGCTGAGTTC CCAGCCTTCA 
CCACGCCTGC GACCATCCAA ATCTATAGAT GAAGGCATGT TCTCAGGTGA TACTTTTATC 
CATCACACAC GCAGTATGCC TCCTGCATTT GGCTTGCCTG AGTACTCCTC ACCTGCTCCT 
CAAGGGGATT ACCAGCCTAA GTCTGTACCT GCTGACTTCT ATGGGGCAGG ATCCAGACAA 
CAGCAGCAGC AGCAGCAGCA GCAAGCCCCC ACCACAACTT TTATTCACCC ACTAACTGGA 
AAAGTACTGG ACCCTTCATC CCCTCTAGGC TTGGCTTTGG CTGCACGGGA ACGTGCCTTG 
AAGGATGATG GACGTATACG TAGGGAAAGG ACCACTGAAC ATCATTTTAC CCGTCAGTTG 
TCAAGCATTG GGGCCTTTTC CTCATCCACT TCACAGCCAA CATCACACAT TTCCTACCCA 
ATGGCCTCGT CTTCCTCTGC AACCTCCTCT GCTGCTCAAT CCACAAACAC CTCTTCATAT 
CTTGTTACCT CCTCATCATT AGCTGCCACC ACCACATCCA CTCGGCCTCC ATCTCCTAGG 
ATCCTGCGTG GAGCAGGGAG TAGCTGGAGT GAAGAAGCTG GGGTTGGAGA GCGTGCGGAT 
AGGGAAGCAG CTGGCACCTC TGCTCCCAGA GAAGGCCTAA GGGTTCGTTT CTCCGAGGAT 
AAAACTGTCC ACACACACCA CTATCAATCT CAGCAATATC AGCCCACCTT TAAAGAGCGT 
GAAAGAGAAC GTCAAAGAGA GAGGTCAAGA GAAAGAGAGA GGGAAAGAGA GAGGGAAAGA 
GAAATAGAAA AAGCTCAAAC CCAGTATCAG CAACAGTCAT CACAACCAAT GACCCAGCAG 
CCTCGACGCC CCTCATTCTT GAGGATGGAG AGTGAAGCTG GAGCGTACAT TCTTTCATTA 
ACGCCTCCAT CTCCTCCTCC AACAGCAGCC CCTATGCGTC CTACAGCAGG AGCCACAGGT 
GAAAGTGGAA GTGGTACAAG CCTGATGGTT CTGCCTCCCC CAGCTCCCTC AGTAGATGCA 
GATGATGAGT TTGTGTTTGC TGATCCTCTT CCTCCACCTC TAGAGTTTGC CAATAGTTTT 
GATCGTGGTT TGGGTTACTC TGCGATGATT TCTCACAATG TTGCAGCCCG TCAGCAGCGT 
CATCCTCCAC CACCCCCTCC CCCACCACCC CCGCCCACTT CGAAAGATTC CTTTATGACA 
GGCTTTCCAG CTCACACACC CCTACCGTCA CCTCAGGCAG GAGATTCCAC AACTTCCAGT 
CTTACCTCCT ATGACAGTGA GGTTGCAAAC CTAACACAAT CTGCCCCATC GCCCTCCCCA 
TCTTCTCCCA ACCCACTACC ACCTCACTCC CCCTCCACCC TTCAGACATC TGGACCACCT 
CCACCTCCCT CTGTATCACC TCCTCCACCA CCCAACAGCT ACCATAGACC TTTTGGCTTA 
TCTCAGTCAC AACATTTATA CAACAGTACA CACACAACTG GACGCTCCTC CTCTCCCACC 
CCACCTCTCT CTATTCCAGC TCCACCTGCC TACCGGGGGC CACCAACAGC ATCATCCACC 
CCTGCAACTT CTGTATCAAT CAGAGGTGCC TCAACTGTGA CCGCAAGCTG TTCCACAACT 
GCTTCTACAA CTGTCACCTC TCCTAGTACT ACTGCTGCTG CCACACCACA CACACGCATA 
GCAACTGTCT CCACAGTAGC TACAGGGGGT AGTAGGCGTC CCAGTGCAGA GCATCATCCA 
CCACCTAGTG CAACCAAGAG TGGTGAGTCC CAGGAGACAG TGGTAGATTC TGGGATTGAG 
GAGCTGGACA GCCGAAGCAG CAGTGATCAT CACTTAGACA GCATTCTTGC ACTCAGTGGT 
AGTGGGGTCA GAGAGAGGTT GGAACGAGCA GGGATGAGTA GCATAGGTGG AATTGGAGGG 
AGAGATGGTG CAACTGAGGT TGACAGGACA AGTGGGGCAG ACTTTTTAGA GTCTTACATG 
AGCTACCTTG ATGGGCAGAC TTTTGAAATG CACAATGCCA AAGCCGCTAG CACTGCATCC 
ACCTACCCAA AAACACAGAG GTACAGAGAA GGCACCCGGG CTGGAGATCT TCGACGCCAG 
ACCAGCACAG CACCTCCTGC ATACCACCGT CGTAGAGAGG AGGAAGAGGA AGAGGATGAG 
GCCGAGGAGG TAGAGGAGGA GGATTTGAGT GCCCGTGATG GCTATGGGAT GAGGGAAGGG 
CCAGGGATTG CTCGCTCCTC TGTAATGTAC TTTGACCGGC CCCGCACTCC AGAGATAAAA 
CCACTGTGGG GAGATGGGTC GACTGGAGCA TCACAGACCA TGGGGGAGGG AGGGGGCCAG 
ATAGTGGGAA CATATGTGGA GCCACGTAAA CTACATCCTC CCATGACTGG TATGAAAGCA 
AACATCATTA ATGAACTAAG CACCAAATTA CAGCAGAGAA GTAAAGGAAC AGAGAACTGG 
GGGGCCCAGA GATCATTGAG CAGACATAGA CACAGGTTCT CAGAGGACTC AACAGGTGCA 
CTGAGTCCTC CCTCTGTCCG CTCTAGCTCC CCATCTCCAG TTCAGCTCTC TCAGCCTGCC 
TTGTCTCCCT CTCCCACTCC TTCCTCCCAG CCACTTTACT CAAACTGGGC TCGATCTCCT 
TCCCCTCAGC CCCCTGCTGC CTCATCCCAG CCCCCTCCCC GATCCCATTC ACCTGTTTCC 
CCTTCATCAT ATTCACCATA CCCCACCTCA CCCAAACACC GGCCTGTTTA TCGCACCAAA 
GCTCTGGAGT TCCAATTTAT TCCCAGTCGA GAGTCACGAA AGTCAGAGTC GCATCATGCC 
CAGCGCAGAC GAGCACCCAG CCCTTTAATT TCTCCATCTG ACCGTCCCAA GCTTGGCCCT 
CCCCGCCCCT CTTCCCTTCC TATTCTCCCC ACTACACCCC TCTATAGCAG CCTGTATGAC 
CTTCGTGGTT CTATTACTCC ACCCTCACCT GCCAATCCCC TTGGGGACCC CTACTTTTCC 
CCCTCCCCCC CTCTATTTGC CCCCTCGGGT GCTCCCCCTC CTCCAAATTC CTCTCTGGTG 
GTCTCACGGT CCCTGTCACC CACTCACTTC CTGTCAGGGA CTTCATCCCC ACCTCTCCAC 
CCCATTCCTC CACCCACTTG CCTGAGTTAC CCCCATCTTC CACCCCCCTC ACCCACAAAG 
CCCTTTGCCT CAAAACCTCT CCCATACTGG ACCAAGTATG ATGTTGCAGA TTGGCTGGGC 
TACCTGAACC TGGGTGAACA CAGGGAGCGA TTCTTAGACA ATGAAATTGA TGGCACGCAC 
CTGCCCTCTC TTACCAAGGA TGACTACCTG GACCTGGGTG TGACCCGAGT TGGCCATCGC 
ATGAACATAG AGAGGGCGCT CAGGAGAGTT ATGGACAGGC TGTCTTCTAG CACTTTCCCC 
ATTTCTGCTC TCTCTCCTCG AAATGGACGG ACAAACAGAA GGAGAGATGA GGCCAACCGG 
AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_021474077.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329752.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X5, mRNA.

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CloneID	ODa141767
Clone ID Related Accession (Same CDS sequence)	XM_021474077.1 , XM_021471435.1
Accession Version	XM_021474077.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGTG 
GACAGAGCCT CTGCCCCATG GAAAAAAAAA TCAGAGTTCG AATCTTCACA AGCTACAGAG 
AAGAAGAGGA CAGTTTATCA GATGGCTTTG AATAAACTGG ATGAGATTTT AGCAGCAGCA 
CAGCAGACCA TTAATACAAA TGAGGCTCCA GGGCCCCGGG TTCAGGGGGT AAAAAGAGAA 
CGGGGCAGAG GCTTTTATAA TGAGCCAAAT TTCGACCAAA CTGGAATGGG GATGACATCA 
TCTGGGGCAG GGTTAGGCTA CGACCGTGGT CAATTTGTGT CTGGCCATGG CCCCCCGCAC 
GGTATGCTGC GACAGAAGTC TATCGGGGTT CCTGAGGAGG AGAAGCAGTT TCTTCATCCT 
CCAGCCATGA AGTTTGCCCG TAGTCTTTCA GTACCTGGCC CAGATGAAAT CCCTCCTCCA 
CCTACTACAG CACCCCCTGA GCCCCCTTTT TCCTCAGCTC CTCCGCTTGG CTTTAGGGGC 
AAGACATCTC AACAGCCGTC TGTTTCTATG TCTCAGTCTA CTTCCTCTGC TGCACATTAT 
CAACTCTACT CACAGTCAGT ACATGTTACT CACGGACCAA GGGACAGGTC CCCTGGACCA 
CCTACTGGGT CTGGAACTGG GGACCTTGCT TATTCCCATG CCCAAGCCCA CCCCTCTGTA 
CCAAGTAGGA CTGCCTCCAG GAAGGTGATT GGGTACACAG GAGATCTTGC AGGGGCTGGA 
GGAGTGGCTG GGGCCAAAGC TGCAGCATTG AGAAGGGGTT ACAGCAATGC AGTCCCACCC 
ACCAGTGTCG CTACTGTGAT TCCACAACAG CAGCAGACTA CTCAGAACCA ACCTTCCAGA 
ATGGACCGGA GTGGAATGGG GGTAGGGGCT GGTGGTGTAC CAAAAGGCGG TGCTCGTAGA 
GGAAAGGGGC CCTTGGTGAA ACAGTCAAAG GTGGAAGACC TGCGTCAAGG TCAAGACACT 
CTAGAAGCAA AGGTGTCAGT GGAGAAGAGC TCTATTCCAA TCCCAACTAT CATTGTTAAA 
GCCCCTTCCA CCAGCAGTAG TGGACGCAGT AGCCAAGGCA GCAGTGTGGA GGCTGAACCA 
CCGCCCCCAG GAGAAAGTGA CAGTTCTAAA CCAGAACCTG TTGTTCCCCC ACCTGCACCA 
ACCGTGCCAC CACCAGCCCC TCCATCTATA CCTGCTCCTC CACCCCCATC ACTGCCATCA 
CTTCGTACTC AAGAGAGTCT GGACTTCACC AGCCAGTTTG GTGCTGCCAT TGTAGGGGCA 
GCTCGCAGGG ACAGAGAACG ATTTCATGAA GCACGACGCA AGAGTGCCTC CTTTTTTCTT 
TCTGCTGAGG AAGAGTTGAG TGGAGCTGGA GGAGCAGGAG GGCGGACTCA GACTTCTCAA 
CAGCAGCTGA GTTCCCAGCC TTCACCACGC CTGCGACCAT CCAAATCTAT AGATGAAGGC 
ATGTTCTCAG GTGATACTTT TATCCATCAC ACACGCAGTA TGCCTCCTGC ATTTGGCTTG 
CCTGAGTACT CCTCACCTGC TCCTCAAGGG GATTACCAGC CTAAGTCTGT ACCTGCTGAC 
TTCTATGGGG CAGGATCCAG ACAACAGCAG CAGCAGCAGC AGCAGCAAGC CCCCACCACA 
ACTTTTATTC ACCCACTAAC TGGAAAAGTA CTGGACCCTT CATCCCCTCT AGGCTTGGCT 
TTGGCTGCAC GGGAACGTGC CTTGAAGGAT GATGGACGTA TACGTAGGGA AAGGACCACT 
GAACATCATT TTACCCGTCA GTTGTCAAGC ATTGGGGCCT TTTCCTCATC CACTTCACAG 
CCAACATCAC ACATTTCCTA CCCAATGGCC TCGTCTTCCT CTGCAACCTC CTCTGCTGCT 
CAATCCACAA ACACCTCTTC ATATCTTGTT ACCTCCTCAT CATTAGCTGC CACCACCACA 
TCCACTCGGC CTCCATCTCC TAGGATCCTG CGTGGAGCAG GGAGTAGCTG GAGTGAAGAA 
GCTGGGGTTG GAGAGCGTGC GGATAGGGAA GCAGCTGGCA CCTCTGCTCC CAGAGAAGGC 
CTAAGGGTTC GTTTCTCCGA GGATAAAACT GTCCACACAC ACCACTATCA ATCTCAGCAA 
TATCAGCCCA CCTTTAAAGA GCGTGAAAGA GAACGTCAAA GAGAGAGGTC AAGAGAAAGA 
GAGAGGGAAA GAGAGAGGGA AAGAGAAATA GAAAAAGCTC AAACCCAGTA TCAGCAACAG 
TCATCACAAC CAATGACCCA GCAGCCTCGA CGCCCCTCAT TCTTGAGGAT GGAGAGTGAA 
GCTGGAGCGT ACATTCTTTC ATTAACGCCT CCATCTCCTC CTCCAACAGC AGCCCCTATG 
CGTCCTACAG CAGGAGCCAC AGGTGAAAGT GGAAGTGGTA CAAGCCTGAT GGTTCTGCCT 
CCCCCAGCTC CCTCAGTAGA TGCAGATGAT GAGTTTGTGT TTGCTGATCC TCTTCCTCCA 
CCTCTAGAGT TTGCCAATAG TTTTGATCGT GGTTTGGGTT ACTCTGCGAT GATTTCTCAC 
AATGTTGCAG CCCGTCAGCA GCGTCATCCT CCACCACCCC CTCCCCCACC ACCCCCGCCC 
ACTTCGAAAG ATTCCTTTAT GACAGGCTTT CCAGCTCACA CACCCCTACC GTCACCTCAG 
GCAGGAGATT CCACAACTTC CAGTCTTACC TCCTATGACA GTGAGGTTGC AAACCTAACA 
CAATCTGCCC CATCGCCCTC CCCATCTTCT CCCAACCCAC TACCACCTCA CTCCCCCTCC 
ACCCTTCAGA CATCTGGACC ACCTCCACCT CCCTCTGTAT CACCTCCTCC ACCACCCAAC 
AGCTACCATA GACCTTTTGG CTTATCTCAG TCACAACATT TATACAACAG TACACACACA 
ACTGGACGCT CCTCCTCTCC CACCCCACCT CTCTCTATTC CAGCTCCACC TGCCTACCGG 
GGGCCACCAA CAGCATCATC CACCCCTGCA ACTTCTGTAT CAATCAGAGG TGCCTCAACT 
GTGACCGCAA GCTGTTCCAC AACTGCTTCT ACAACTGTCA CCTCTCCTAG TACTACTGCT 
GCTGCCACAC CACACACACG CATAGCAACT GTCTCCACAG TAGCTACAGG GGGTAGTAGG 
CGTCCCAGTG CAGAGCATCA TCCACCACCT AGTGCAACCA AGAGTGGTGA GTCCCAGGAG 
ACAGTGGTAG ATTCTGGGAT TGAGGAGCTG GACAGCCGAA GCAGCAGTGA TCATCACTTA 
GACAGCATTC TTGCACTCAG TGGTAGTGGG GTCAGAGAGA GGTTGGAACG AGCAGGGATG 
AGTAGCATAG GTGGAATTGG AGGGAGAGAT GGTGCAACTG AGGTTGACAG GACAAGTGGG 
GCAGACTTTT TAGAGTCTTA CATGAGCTAC CTTGATGGGC AGACTTTTGA AATGCACAAT 
GCCAAAGCCG CTAGCACTGC ATCCACCTAC CCAAAAACAC AGAGGTACAG AGAAGGCACC 
CGGGCTGGAG ATCTTCGACG CCAGACCAGC ACAGCACCTC CTGCATACCA CCGTCGTAGA 
GAGGAGGAAG AGGAAGAGGA TGAGGCCGAG GAGGTAGAGG AGGAGGATTT GAGTGCCCGT 
GATGGCTATG GGATGAGGGA AGGGCCAGGG ATTGCTCGCT CCTCTGTAAT GTACTTTGAC 
CGGCCCCGCA CTCCAGAGAT AAAACCACTG TGGGGAGATG GGTCGACTGG AGCATCACAG 
ACCATGGGGG AGGGAGGGGG CCAGATAGTG GGAACATATG TGGAGCCACG TAAACTACAT 
CCTCCCATGA CTGGTATGAA AGCAAACATC ATTAATGAAC TAAGCACCAA ATTACAGCAG 
AGAAGTAAAG GAACAGAGAA CTGGGGGGCC CAGAGATCAT TGAGCAGACA TAGACACAGG 
TTCTCAGAGG ACTCAACAGG TGCACTGAGT CCTCCCTCTG TCCGCTCTAG CTCCCCATCT 
CCAGTTCAGC TCTCTCAGCC TGCCTTGTCT CCCTCTCCCA CTCCTTCCTC CCAGCCACTT 
TACTCAAACT GGGCTCGATC TCCTTCCCCT CAGCCCCCTG CTGCCTCATC CCAGCCCCCT 
CCCCGATCCC ATTCACCTGT TTCCCCTTCA TCATATTCAC CATACCCCAC CTCACCCAAA 
CACCGGCCTG TTTATCGCAC CAAAGCTCTG GAGTTCCAAT TTATTCCCAG TCGAGAGTCA 
CGAAAGTCAG AGTCGCATCA TGCCCAGCGC AGACGAGCAC CCAGCCCTTT AATTTCTCCA 
TCTGACCGTC CCAAGCTTGG CCCTCCCCGC CCCTCTTCCC TTCCTATTCT CCCCACTACA 
CCCCTCTATA GCAGCCTGTA TGACCTTCGT GGTTCTATTA CTCCACCCTC ACCTGCCAAT 
CCCCTTGGGG ACCCCTACTT TTCCCCCTCC CCCCCTCTAT TTGCCCCCTC GGGTGCTCCC 
CCTCCTCCAA ATTCCTCTCT GGTGGTCTCA CGGTCCCTGT CACCCACTCA CTTCCTGTCA 
GGGACTTCAT CCCCACCTCT CCACCCCATT CCTCCACCCA CTTGCCTGAG TTACCCCCAT 
CTTCCACCCC CCTCACCCAC AAAGCCCTTT GCCTCAAAAC CTCTCCCATA CTGGACCAAG 
TATGATGTTG CAGATTGGCT GGGCTACCTG AACCTGGGTG AACACAGGGA GCGATTCTTA 
GACAATGAAA TTGATGGCAC GCACCTGCCC TCTCTTACCA AGGATGACTA CCTGGACCTG 
GGTGTGACCC GAGTTGGCCA TCGCATGAAC ATAGAGAGGG CGCTCAGGAG AGTTATGGAC 
AGAGAACACT TGGAGTGGGC CACTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329752.1
CDS	381..7730
Translation	MTMPLSPLSSDEEQQRMLGNSQHLYPGAEEEEEEEEEMDDDGMDEEGEEENGELEMDDEEEVDDEDDIRRGPGHLRGGREEGHRQKGTMAGRLPGDRPIRPGNPGHAANPYSSNPGPTHQTSTNQQQQQPQRKLRERSSSNAPDDTHVALMVFRIGIPDIKQTKCLRFNPDATVWKAKQQVMCSLTESLRDVLNYGLFQPATDGHDAKFLEEERPLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKASLKKFMDYIQTSAVEKVAKLIDKGLDPNYHDPDTGETPLTLAVQTEPGGSEIIRVLVMGGAHIDFRARDGLTPLHKAVRAHAHTALLTLLSLGASSDYKDSRGLTPLYHTVLIGGDTSCCETLLYHRARLGLRDENGWDETHQACQNGNSQHLEHLLFYGADSSSQNASGNTALHICALYNKESCARILLYRGAKKDTKNNSGQNSFQVAVMSGHFELGEIIKNHRESDVVPFLESPKYAPQRRESSRTLGLPHPHPFLRANSDNSMNVPDWIAFPNAAGSSIVSVQGLKHGGTLRSSSSPRGARTRSPSRGRTGDRDDRSRQTRGRQGAGSISSQGTASGQRRRLYSAVPGRVFVATRSHSAQGDREISFNKGDKVKVLSVGEGGYWEGTVRGRTGWFPSDCVEEVALRSLESRSESRSERAKRLFRHYTVGSYDSFDAPSDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMVDRASAPWKKKSEFESSQATEKKRTVYQMALNKLDEILAAAQQTINTNEAPGPRVQGVKRERGRGFYNEPNFDQTGMGMTSSGAGLGYDRGQFVSGHGPPHGMLRQKSIGVPEEEKQFLHPPAMKFARSLSVPGPDEIPPPPTTAPPEPPFSSAPPLGFRGKTSQQPSVSMSQSTSSAAHYQLYSQSVHVTHGPRDRSPGPPTGSGTGDLAYSHAQAHPSVPSRTASRKVIGYTGDLAGAGGVAGAKAAALRRGYSNAVPPTSVATVIPQQQQTTQNQPSRMDRSGMGVGAGGVPKGGARRGKGPLVKQSKVEDLRQGQDTLEAKVSVEKSSIPIPTIIVKAPSTSSSGRSSQGSSVEAEPPPPGESDSSKPEPVVPPPAPTVPPPAPPSIPAPPPPSLPSLRTQESLDFTSQFGAAIVGAARRDRERFHEARRKSASFFLSAEEELSGAGGAGGRTQTSQQQLSSQPSPRLRPSKSIDEGMFSGDTFIHHTRSMPPAFGLPEYSSPAPQGDYQPKSVPADFYGAGSRQQQQQQQQQAPTTTFIHPLTGKVLDPSSPLGLALAARERALKDDGRIRRERTTEHHFTRQLSSIGAFSSSTSQPTSHISYPMASSSSATSSAAQSTNTSSYLVTSSSLAATTTSTRPPSPRILRGAGSSWSEEAGVGERADREAAGTSAPREGLRVRFSEDKTVHTHHYQSQQYQPTFKERERERQRERSREREREREREREIEKAQTQYQQQSSQPMTQQPRRPSFLRMESEAGAYILSLTPPSPPPTAAPMRPTAGATGESGSGTSLMVLPPPAPSVDADDEFVFADPLPPPLEFANSFDRGLGYSAMISHNVAARQQRHPPPPPPPPPPPTSKDSFMTGFPAHTPLPSPQAGDSTTSSLTSYDSEVANLTQSAPSPSPSSPNPLPPHSPSTLQTSGPPPPPSVSPPPPPNSYHRPFGLSQSQHLYNSTHTTGRSSSPTPPLSIPAPPAYRGPPTASSTPATSVSIRGASTVTASCSTTASTTVTSPSTTAAATPHTRIATVSTVATGGSRRPSAEHHPPPSATKSGESQETVVDSGIEELDSRSSSDHHLDSILALSGSGVRERLERAGMSSIGGIGGRDGATEVDRTSGADFLESYMSYLDGQTFEMHNAKAASTASTYPKTQRYREGTRAGDLRRQTSTAPPAYHRRREEEEEEDEAEEVEEEDLSARDGYGMREGPGIARSSVMYFDRPRTPEIKPLWGDGSTGASQTMGEGGGQIVGTYVEPRKLHPPMTGMKANIINELSTKLQQRSKGTENWGAQRSLSRHRHRFSEDSTGALSPPSVRSSSPSPVQLSQPALSPSPTPSSQPLYSNWARSPSPQPPAASSQPPPRSHSPVSPSSYSPYPTSPKHRPVYRTKALEFQFIPSRESRKSESHHAQRRRAPSPLISPSDRPKLGPPRPSSLPILPTTPLYSSLYDLRGSITPPSPANPLGDPYFSPSPPLFAPSGAPPPPNSSLVVSRSLSPTHFLSGTSSPPLHPIPPPTCLSYPHLPPPSPTKPFASKPLPYWTKYDVADWLGYLNLGEHRERFLDNEIDGTHLPSLTKDDYLDLGVTRVGHRMNIERALRRVMDREHLEWATA

Target ORF information:

RefSeq Version	XM_021474077.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474077.1

ORF Insert Sequence:

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGTG 
GACAGAGCCT CTGCCCCATG GAAAAAAAAA TCAGAGTTCG AATCTTCACA AGCTACAGAG 
AAGAAGAGGA CAGTTTATCA GATGGCTTTG AATAAACTGG ATGAGATTTT AGCAGCAGCA 
CAGCAGACCA TTAATACAAA TGAGGCTCCA GGGCCCCGGG TTCAGGGGGT AAAAAGAGAA 
CGGGGCAGAG GCTTTTATAA TGAGCCAAAT TTCGACCAAA CTGGAATGGG GATGACATCA 
TCTGGGGCAG GGTTAGGCTA CGACCGTGGT CAATTTGTGT CTGGCCATGG CCCCCCGCAC 
GGTATGCTGC GACAGAAGTC TATCGGGGTT CCTGAGGAGG AGAAGCAGTT TCTTCATCCT 
CCAGCCATGA AGTTTGCCCG TAGTCTTTCA GTACCTGGCC CAGATGAAAT CCCTCCTCCA 
CCTACTACAG CACCCCCTGA GCCCCCTTTT TCCTCAGCTC CTCCGCTTGG CTTTAGGGGC 
AAGACATCTC AACAGCCGTC TGTTTCTATG TCTCAGTCTA CTTCCTCTGC TGCACATTAT 
CAACTCTACT CACAGTCAGT ACATGTTACT CACGGACCAA GGGACAGGTC CCCTGGACCA 
CCTACTGGGT CTGGAACTGG GGACCTTGCT TATTCCCATG CCCAAGCCCA CCCCTCTGTA 
CCAAGTAGGA CTGCCTCCAG GAAGGTGATT GGGTACACAG GAGATCTTGC AGGGGCTGGA 
GGAGTGGCTG GGGCCAAAGC TGCAGCATTG AGAAGGGGTT ACAGCAATGC AGTCCCACCC 
ACCAGTGTCG CTACTGTGAT TCCACAACAG CAGCAGACTA CTCAGAACCA ACCTTCCAGA 
ATGGACCGGA GTGGAATGGG GGTAGGGGCT GGTGGTGTAC CAAAAGGCGG TGCTCGTAGA 
GGAAAGGGGC CCTTGGTGAA ACAGTCAAAG GTGGAAGACC TGCGTCAAGG TCAAGACACT 
CTAGAAGCAA AGGTGTCAGT GGAGAAGAGC TCTATTCCAA TCCCAACTAT CATTGTTAAA 
GCCCCTTCCA CCAGCAGTAG TGGACGCAGT AGCCAAGGCA GCAGTGTGGA GGCTGAACCA 
CCGCCCCCAG GAGAAAGTGA CAGTTCTAAA CCAGAACCTG TTGTTCCCCC ACCTGCACCA 
ACCGTGCCAC CACCAGCCCC TCCATCTATA CCTGCTCCTC CACCCCCATC ACTGCCATCA 
CTTCGTACTC AAGAGAGTCT GGACTTCACC AGCCAGTTTG GTGCTGCCAT TGTAGGGGCA 
GCTCGCAGGG ACAGAGAACG ATTTCATGAA GCACGACGCA AGAGTGCCTC CTTTTTTCTT 
TCTGCTGAGG AAGAGTTGAG TGGAGCTGGA GGAGCAGGAG GGCGGACTCA GACTTCTCAA 
CAGCAGCTGA GTTCCCAGCC TTCACCACGC CTGCGACCAT CCAAATCTAT AGATGAAGGC 
ATGTTCTCAG GTGATACTTT TATCCATCAC ACACGCAGTA TGCCTCCTGC ATTTGGCTTG 
CCTGAGTACT CCTCACCTGC TCCTCAAGGG GATTACCAGC CTAAGTCTGT ACCTGCTGAC 
TTCTATGGGG CAGGATCCAG ACAACAGCAG CAGCAGCAGC AGCAGCAAGC CCCCACCACA 
ACTTTTATTC ACCCACTAAC TGGAAAAGTA CTGGACCCTT CATCCCCTCT AGGCTTGGCT 
TTGGCTGCAC GGGAACGTGC CTTGAAGGAT GATGGACGTA TACGTAGGGA AAGGACCACT 
GAACATCATT TTACCCGTCA GTTGTCAAGC ATTGGGGCCT TTTCCTCATC CACTTCACAG 
CCAACATCAC ACATTTCCTA CCCAATGGCC TCGTCTTCCT CTGCAACCTC CTCTGCTGCT 
CAATCCACAA ACACCTCTTC ATATCTTGTT ACCTCCTCAT CATTAGCTGC CACCACCACA 
TCCACTCGGC CTCCATCTCC TAGGATCCTG CGTGGAGCAG GGAGTAGCTG GAGTGAAGAA 
GCTGGGGTTG GAGAGCGTGC GGATAGGGAA GCAGCTGGCA CCTCTGCTCC CAGAGAAGGC 
CTAAGGGTTC GTTTCTCCGA GGATAAAACT GTCCACACAC ACCACTATCA ATCTCAGCAA 
TATCAGCCCA CCTTTAAAGA GCGTGAAAGA GAACGTCAAA GAGAGAGGTC AAGAGAAAGA 
GAGAGGGAAA GAGAGAGGGA AAGAGAAATA GAAAAAGCTC AAACCCAGTA TCAGCAACAG 
TCATCACAAC CAATGACCCA GCAGCCTCGA CGCCCCTCAT TCTTGAGGAT GGAGAGTGAA 
GCTGGAGCGT ACATTCTTTC ATTAACGCCT CCATCTCCTC CTCCAACAGC AGCCCCTATG 
CGTCCTACAG CAGGAGCCAC AGGTGAAAGT GGAAGTGGTA CAAGCCTGAT GGTTCTGCCT 
CCCCCAGCTC CCTCAGTAGA TGCAGATGAT GAGTTTGTGT TTGCTGATCC TCTTCCTCCA 
CCTCTAGAGT TTGCCAATAG TTTTGATCGT GGTTTGGGTT ACTCTGCGAT GATTTCTCAC 
AATGTTGCAG CCCGTCAGCA GCGTCATCCT CCACCACCCC CTCCCCCACC ACCCCCGCCC 
ACTTCGAAAG ATTCCTTTAT GACAGGCTTT CCAGCTCACA CACCCCTACC GTCACCTCAG 
GCAGGAGATT CCACAACTTC CAGTCTTACC TCCTATGACA GTGAGGTTGC AAACCTAACA 
CAATCTGCCC CATCGCCCTC CCCATCTTCT CCCAACCCAC TACCACCTCA CTCCCCCTCC 
ACCCTTCAGA CATCTGGACC ACCTCCACCT CCCTCTGTAT CACCTCCTCC ACCACCCAAC 
AGCTACCATA GACCTTTTGG CTTATCTCAG TCACAACATT TATACAACAG TACACACACA 
ACTGGACGCT CCTCCTCTCC CACCCCACCT CTCTCTATTC CAGCTCCACC TGCCTACCGG 
GGGCCACCAA CAGCATCATC CACCCCTGCA ACTTCTGTAT CAATCAGAGG TGCCTCAACT 
GTGACCGCAA GCTGTTCCAC AACTGCTTCT ACAACTGTCA CCTCTCCTAG TACTACTGCT 
GCTGCCACAC CACACACACG CATAGCAACT GTCTCCACAG TAGCTACAGG GGGTAGTAGG 
CGTCCCAGTG CAGAGCATCA TCCACCACCT AGTGCAACCA AGAGTGGTGA GTCCCAGGAG 
ACAGTGGTAG ATTCTGGGAT TGAGGAGCTG GACAGCCGAA GCAGCAGTGA TCATCACTTA 
GACAGCATTC TTGCACTCAG TGGTAGTGGG GTCAGAGAGA GGTTGGAACG AGCAGGGATG 
AGTAGCATAG GTGGAATTGG AGGGAGAGAT GGTGCAACTG AGGTTGACAG GACAAGTGGG 
GCAGACTTTT TAGAGTCTTA CATGAGCTAC CTTGATGGGC AGACTTTTGA AATGCACAAT 
GCCAAAGCCG CTAGCACTGC ATCCACCTAC CCAAAAACAC AGAGGTACAG AGAAGGCACC 
CGGGCTGGAG ATCTTCGACG CCAGACCAGC ACAGCACCTC CTGCATACCA CCGTCGTAGA 
GAGGAGGAAG AGGAAGAGGA TGAGGCCGAG GAGGTAGAGG AGGAGGATTT GAGTGCCCGT 
GATGGCTATG GGATGAGGGA AGGGCCAGGG ATTGCTCGCT CCTCTGTAAT GTACTTTGAC 
CGGCCCCGCA CTCCAGAGAT AAAACCACTG TGGGGAGATG GGTCGACTGG AGCATCACAG 
ACCATGGGGG AGGGAGGGGG CCAGATAGTG GGAACATATG TGGAGCCACG TAAACTACAT 
CCTCCCATGA CTGGTATGAA AGCAAACATC ATTAATGAAC TAAGCACCAA ATTACAGCAG 
AGAAGTAAAG GAACAGAGAA CTGGGGGGCC CAGAGATCAT TGAGCAGACA TAGACACAGG 
TTCTCAGAGG ACTCAACAGG TGCACTGAGT CCTCCCTCTG TCCGCTCTAG CTCCCCATCT 
CCAGTTCAGC TCTCTCAGCC TGCCTTGTCT CCCTCTCCCA CTCCTTCCTC CCAGCCACTT 
TACTCAAACT GGGCTCGATC TCCTTCCCCT CAGCCCCCTG CTGCCTCATC CCAGCCCCCT 
CCCCGATCCC ATTCACCTGT TTCCCCTTCA TCATATTCAC CATACCCCAC CTCACCCAAA 
CACCGGCCTG TTTATCGCAC CAAAGCTCTG GAGTTCCAAT TTATTCCCAG TCGAGAGTCA 
CGAAAGTCAG AGTCGCATCA TGCCCAGCGC AGACGAGCAC CCAGCCCTTT AATTTCTCCA 
TCTGACCGTC CCAAGCTTGG CCCTCCCCGC CCCTCTTCCC TTCCTATTCT CCCCACTACA 
CCCCTCTATA GCAGCCTGTA TGACCTTCGT GGTTCTATTA CTCCACCCTC ACCTGCCAAT 
CCCCTTGGGG ACCCCTACTT TTCCCCCTCC CCCCCTCTAT TTGCCCCCTC GGGTGCTCCC 
CCTCCTCCAA ATTCCTCTCT GGTGGTCTCA CGGTCCCTGT CACCCACTCA CTTCCTGTCA 
GGGACTTCAT CCCCACCTCT CCACCCCATT CCTCCACCCA CTTGCCTGAG TTACCCCCAT 
CTTCCACCCC CCTCACCCAC AAAGCCCTTT GCCTCAAAAC CTCTCCCATA CTGGACCAAG 
TATGATGTTG CAGATTGGCT GGGCTACCTG AACCTGGGTG AACACAGGGA GCGATTCTTA 
GACAATGAAA TTGATGGCAC GCACCTGCCC TCTCTTACCA AGGATGACTA CCTGGACCTG 
GGTGTGACCC GAGTTGGCCA TCGCATGAAC ATAGAGAGGG CGCTCAGGAG AGTTATGGAC 
AGAGAACACT TGGAGTGGGC CACTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_021471435.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327110.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X5, mRNA.

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CloneID	ODa141767
Clone ID Related Accession (Same CDS sequence)	XM_021474077.1 , XM_021471435.1
Accession Version	XM_021471435.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394533.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGTG 
GACAGAGCCT CTGCCCCATG GAAAAAAAAA TCAGAGTTCG AATCTTCACA AGCTACAGAG 
AAGAAGAGGA CAGTTTATCA GATGGCTTTG AATAAACTGG ATGAGATTTT AGCAGCAGCA 
CAGCAGACCA TTAATACAAA TGAGGCTCCA GGGCCCCGGG TTCAGGGGGT AAAAAGAGAA 
CGGGGCAGAG GCTTTTATAA TGAGCCAAAT TTCGACCAAA CTGGAATGGG GATGACATCA 
TCTGGGGCAG GGTTAGGCTA CGACCGTGGT CAATTTGTGT CTGGCCATGG CCCCCCGCAC 
GGTATGCTGC GACAGAAGTC TATCGGGGTT CCTGAGGAGG AGAAGCAGTT TCTTCATCCT 
CCAGCCATGA AGTTTGCCCG TAGTCTTTCA GTACCTGGCC CAGATGAAAT CCCTCCTCCA 
CCTACTACAG CACCCCCTGA GCCCCCTTTT TCCTCAGCTC CTCCGCTTGG CTTTAGGGGC 
AAGACATCTC AACAGCCGTC TGTTTCTATG TCTCAGTCTA CTTCCTCTGC TGCACATTAT 
CAACTCTACT CACAGTCAGT ACATGTTACT CACGGACCAA GGGACAGGTC CCCTGGACCA 
CCTACTGGGT CTGGAACTGG GGACCTTGCT TATTCCCATG CCCAAGCCCA CCCCTCTGTA 
CCAAGTAGGA CTGCCTCCAG GAAGGTGATT GGGTACACAG GAGATCTTGC AGGGGCTGGA 
GGAGTGGCTG GGGCCAAAGC TGCAGCATTG AGAAGGGGTT ACAGCAATGC AGTCCCACCC 
ACCAGTGTCG CTACTGTGAT TCCACAACAG CAGCAGACTA CTCAGAACCA ACCTTCCAGA 
ATGGACCGGA GTGGAATGGG GGTAGGGGCT GGTGGTGTAC CAAAAGGCGG TGCTCGTAGA 
GGAAAGGGGC CCTTGGTGAA ACAGTCAAAG GTGGAAGACC TGCGTCAAGG TCAAGACACT 
CTAGAAGCAA AGGTGTCAGT GGAGAAGAGC TCTATTCCAA TCCCAACTAT CATTGTTAAA 
GCCCCTTCCA CCAGCAGTAG TGGACGCAGT AGCCAAGGCA GCAGTGTGGA GGCTGAACCA 
CCGCCCCCAG GAGAAAGTGA CAGTTCTAAA CCAGAACCTG TTGTTCCCCC ACCTGCACCA 
ACCGTGCCAC CACCAGCCCC TCCATCTATA CCTGCTCCTC CACCCCCATC ACTGCCATCA 
CTTCGTACTC AAGAGAGTCT GGACTTCACC AGCCAGTTTG GTGCTGCCAT TGTAGGGGCA 
GCTCGCAGGG ACAGAGAACG ATTTCATGAA GCACGACGCA AGAGTGCCTC CTTTTTTCTT 
TCTGCTGAGG AAGAGTTGAG TGGAGCTGGA GGAGCAGGAG GGCGGACTCA GACTTCTCAA 
CAGCAGCTGA GTTCCCAGCC TTCACCACGC CTGCGACCAT CCAAATCTAT AGATGAAGGC 
ATGTTCTCAG GTGATACTTT TATCCATCAC ACACGCAGTA TGCCTCCTGC ATTTGGCTTG 
CCTGAGTACT CCTCACCTGC TCCTCAAGGG GATTACCAGC CTAAGTCTGT ACCTGCTGAC 
TTCTATGGGG CAGGATCCAG ACAACAGCAG CAGCAGCAGC AGCAGCAAGC CCCCACCACA 
ACTTTTATTC ACCCACTAAC TGGAAAAGTA CTGGACCCTT CATCCCCTCT AGGCTTGGCT 
TTGGCTGCAC GGGAACGTGC CTTGAAGGAT GATGGACGTA TACGTAGGGA AAGGACCACT 
GAACATCATT TTACCCGTCA GTTGTCAAGC ATTGGGGCCT TTTCCTCATC CACTTCACAG 
CCAACATCAC ACATTTCCTA CCCAATGGCC TCGTCTTCCT CTGCAACCTC CTCTGCTGCT 
CAATCCACAA ACACCTCTTC ATATCTTGTT ACCTCCTCAT CATTAGCTGC CACCACCACA 
TCCACTCGGC CTCCATCTCC TAGGATCCTG CGTGGAGCAG GGAGTAGCTG GAGTGAAGAA 
GCTGGGGTTG GAGAGCGTGC GGATAGGGAA GCAGCTGGCA CCTCTGCTCC CAGAGAAGGC 
CTAAGGGTTC GTTTCTCCGA GGATAAAACT GTCCACACAC ACCACTATCA ATCTCAGCAA 
TATCAGCCCA CCTTTAAAGA GCGTGAAAGA GAACGTCAAA GAGAGAGGTC AAGAGAAAGA 
GAGAGGGAAA GAGAGAGGGA AAGAGAAATA GAAAAAGCTC AAACCCAGTA TCAGCAACAG 
TCATCACAAC CAATGACCCA GCAGCCTCGA CGCCCCTCAT TCTTGAGGAT GGAGAGTGAA 
GCTGGAGCGT ACATTCTTTC ATTAACGCCT CCATCTCCTC CTCCAACAGC AGCCCCTATG 
CGTCCTACAG CAGGAGCCAC AGGTGAAAGT GGAAGTGGTA CAAGCCTGAT GGTTCTGCCT 
CCCCCAGCTC CCTCAGTAGA TGCAGATGAT GAGTTTGTGT TTGCTGATCC TCTTCCTCCA 
CCTCTAGAGT TTGCCAATAG TTTTGATCGT GGTTTGGGTT ACTCTGCGAT GATTTCTCAC 
AATGTTGCAG CCCGTCAGCA GCGTCATCCT CCACCACCCC CTCCCCCACC ACCCCCGCCC 
ACTTCGAAAG ATTCCTTTAT GACAGGCTTT CCAGCTCACA CACCCCTACC GTCACCTCAG 
GCAGGAGATT CCACAACTTC CAGTCTTACC TCCTATGACA GTGAGGTTGC AAACCTAACA 
CAATCTGCCC CATCGCCCTC CCCATCTTCT CCCAACCCAC TACCACCTCA CTCCCCCTCC 
ACCCTTCAGA CATCTGGACC ACCTCCACCT CCCTCTGTAT CACCTCCTCC ACCACCCAAC 
AGCTACCATA GACCTTTTGG CTTATCTCAG TCACAACATT TATACAACAG TACACACACA 
ACTGGACGCT CCTCCTCTCC CACCCCACCT CTCTCTATTC CAGCTCCACC TGCCTACCGG 
GGGCCACCAA CAGCATCATC CACCCCTGCA ACTTCTGTAT CAATCAGAGG TGCCTCAACT 
GTGACCGCAA GCTGTTCCAC AACTGCTTCT ACAACTGTCA CCTCTCCTAG TACTACTGCT 
GCTGCCACAC CACACACACG CATAGCAACT GTCTCCACAG TAGCTACAGG GGGTAGTAGG 
CGTCCCAGTG CAGAGCATCA TCCACCACCT AGTGCAACCA AGAGTGGTGA GTCCCAGGAG 
ACAGTGGTAG ATTCTGGGAT TGAGGAGCTG GACAGCCGAA GCAGCAGTGA TCATCACTTA 
GACAGCATTC TTGCACTCAG TGGTAGTGGG GTCAGAGAGA GGTTGGAACG AGCAGGGATG 
AGTAGCATAG GTGGAATTGG AGGGAGAGAT GGTGCAACTG AGGTTGACAG GACAAGTGGG 
GCAGACTTTT TAGAGTCTTA CATGAGCTAC CTTGATGGGC AGACTTTTGA AATGCACAAT 
GCCAAAGCCG CTAGCACTGC ATCCACCTAC CCAAAAACAC AGAGGTACAG AGAAGGCACC 
CGGGCTGGAG ATCTTCGACG CCAGACCAGC ACAGCACCTC CTGCATACCA CCGTCGTAGA 
GAGGAGGAAG AGGAAGAGGA TGAGGCCGAG GAGGTAGAGG AGGAGGATTT GAGTGCCCGT 
GATGGCTATG GGATGAGGGA AGGGCCAGGG ATTGCTCGCT CCTCTGTAAT GTACTTTGAC 
CGGCCCCGCA CTCCAGAGAT AAAACCACTG TGGGGAGATG GGTCGACTGG AGCATCACAG 
ACCATGGGGG AGGGAGGGGG CCAGATAGTG GGAACATATG TGGAGCCACG TAAACTACAT 
CCTCCCATGA CTGGTATGAA AGCAAACATC ATTAATGAAC TAAGCACCAA ATTACAGCAG 
AGAAGTAAAG GAACAGAGAA CTGGGGGGCC CAGAGATCAT TGAGCAGACA TAGACACAGG 
TTCTCAGAGG ACTCAACAGG TGCACTGAGT CCTCCCTCTG TCCGCTCTAG CTCCCCATCT 
CCAGTTCAGC TCTCTCAGCC TGCCTTGTCT CCCTCTCCCA CTCCTTCCTC CCAGCCACTT 
TACTCAAACT GGGCTCGATC TCCTTCCCCT CAGCCCCCTG CTGCCTCATC CCAGCCCCCT 
CCCCGATCCC ATTCACCTGT TTCCCCTTCA TCATATTCAC CATACCCCAC CTCACCCAAA 
CACCGGCCTG TTTATCGCAC CAAAGCTCTG GAGTTCCAAT TTATTCCCAG TCGAGAGTCA 
CGAAAGTCAG AGTCGCATCA TGCCCAGCGC AGACGAGCAC CCAGCCCTTT AATTTCTCCA 
TCTGACCGTC CCAAGCTTGG CCCTCCCCGC CCCTCTTCCC TTCCTATTCT CCCCACTACA 
CCCCTCTATA GCAGCCTGTA TGACCTTCGT GGTTCTATTA CTCCACCCTC ACCTGCCAAT 
CCCCTTGGGG ACCCCTACTT TTCCCCCTCC CCCCCTCTAT TTGCCCCCTC GGGTGCTCCC 
CCTCCTCCAA ATTCCTCTCT GGTGGTCTCA CGGTCCCTGT CACCCACTCA CTTCCTGTCA 
GGGACTTCAT CCCCACCTCT CCACCCCATT CCTCCACCCA CTTGCCTGAG TTACCCCCAT 
CTTCCACCCC CCTCACCCAC AAAGCCCTTT GCCTCAAAAC CTCTCCCATA CTGGACCAAG 
TATGATGTTG CAGATTGGCT GGGCTACCTG AACCTGGGTG AACACAGGGA GCGATTCTTA 
GACAATGAAA TTGATGGCAC GCACCTGCCC TCTCTTACCA AGGATGACTA CCTGGACCTG 
GGTGTGACCC GAGTTGGCCA TCGCATGAAC ATAGAGAGGG CGCTCAGGAG AGTTATGGAC 
AGAGAACACT TGGAGTGGGC CACTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327110.1
CDS	381..7730
Translation	MTMPLSPLSSDEEQQRMLGNSQHLYPGAEEEEEEEEEMDDDGMDEEGEEENGELEMDDEEEVDDEDDIRRGPGHLRGGREEGHRQKGTMAGRLPGDRPIRPGNPGHAANPYSSNPGPTHQTSTNQQQQQPQRKLRERSSSNAPDDTHVALMVFRIGIPDIKQTKCLRFNPDATVWKAKQQVMCSLTESLRDVLNYGLFQPATDGHDAKFLEEERPLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKASLKKFMDYIQTSAVEKVAKLIDKGLDPNYHDPDTGETPLTLAVQTEPGGSEIIRVLVMGGAHIDFRARDGLTPLHKAVRAHAHTALLTLLSLGASSDYKDSRGLTPLYHTVLIGGDTSCCETLLYHRARLGLRDENGWDETHQACQNGNSQHLEHLLFYGADSSSQNASGNTALHICALYNKESCARILLYRGAKKDTKNNSGQNSFQVAVMSGHFELGEIIKNHRESDVVPFLESPKYAPQRRESSRTLGLPHPHPFLRANSDNSMNVPDWIAFPNAAGSSIVSVQGLKHGGTLRSSSSPRGARTRSPSRGRTGDRDDRSRQTRGRQGAGSISSQGTASGQRRRLYSAVPGRVFVATRSHSAQGDREISFNKGDKVKVLSVGEGGYWEGTVRGRTGWFPSDCVEEVALRSLESRSESRSERAKRLFRHYTVGSYDSFDAPSDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMVDRASAPWKKKSEFESSQATEKKRTVYQMALNKLDEILAAAQQTINTNEAPGPRVQGVKRERGRGFYNEPNFDQTGMGMTSSGAGLGYDRGQFVSGHGPPHGMLRQKSIGVPEEEKQFLHPPAMKFARSLSVPGPDEIPPPPTTAPPEPPFSSAPPLGFRGKTSQQPSVSMSQSTSSAAHYQLYSQSVHVTHGPRDRSPGPPTGSGTGDLAYSHAQAHPSVPSRTASRKVIGYTGDLAGAGGVAGAKAAALRRGYSNAVPPTSVATVIPQQQQTTQNQPSRMDRSGMGVGAGGVPKGGARRGKGPLVKQSKVEDLRQGQDTLEAKVSVEKSSIPIPTIIVKAPSTSSSGRSSQGSSVEAEPPPPGESDSSKPEPVVPPPAPTVPPPAPPSIPAPPPPSLPSLRTQESLDFTSQFGAAIVGAARRDRERFHEARRKSASFFLSAEEELSGAGGAGGRTQTSQQQLSSQPSPRLRPSKSIDEGMFSGDTFIHHTRSMPPAFGLPEYSSPAPQGDYQPKSVPADFYGAGSRQQQQQQQQQAPTTTFIHPLTGKVLDPSSPLGLALAARERALKDDGRIRRERTTEHHFTRQLSSIGAFSSSTSQPTSHISYPMASSSSATSSAAQSTNTSSYLVTSSSLAATTTSTRPPSPRILRGAGSSWSEEAGVGERADREAAGTSAPREGLRVRFSEDKTVHTHHYQSQQYQPTFKERERERQRERSREREREREREREIEKAQTQYQQQSSQPMTQQPRRPSFLRMESEAGAYILSLTPPSPPPTAAPMRPTAGATGESGSGTSLMVLPPPAPSVDADDEFVFADPLPPPLEFANSFDRGLGYSAMISHNVAARQQRHPPPPPPPPPPPTSKDSFMTGFPAHTPLPSPQAGDSTTSSLTSYDSEVANLTQSAPSPSPSSPNPLPPHSPSTLQTSGPPPPPSVSPPPPPNSYHRPFGLSQSQHLYNSTHTTGRSSSPTPPLSIPAPPAYRGPPTASSTPATSVSIRGASTVTASCSTTASTTVTSPSTTAAATPHTRIATVSTVATGGSRRPSAEHHPPPSATKSGESQETVVDSGIEELDSRSSSDHHLDSILALSGSGVRERLERAGMSSIGGIGGRDGATEVDRTSGADFLESYMSYLDGQTFEMHNAKAASTASTYPKTQRYREGTRAGDLRRQTSTAPPAYHRRREEEEEEDEAEEVEEEDLSARDGYGMREGPGIARSSVMYFDRPRTPEIKPLWGDGSTGASQTMGEGGGQIVGTYVEPRKLHPPMTGMKANIINELSTKLQQRSKGTENWGAQRSLSRHRHRFSEDSTGALSPPSVRSSSPSPVQLSQPALSPSPTPSSQPLYSNWARSPSPQPPAASSQPPPRSHSPVSPSSYSPYPTSPKHRPVYRTKALEFQFIPSRESRKSESHHAQRRRAPSPLISPSDRPKLGPPRPSSLPILPTTPLYSSLYDLRGSITPPSPANPLGDPYFSPSPPLFAPSGAPPPPNSSLVVSRSLSPTHFLSGTSSPPLHPIPPPTCLSYPHLPPPSPTKPFASKPLPYWTKYDVADWLGYLNLGEHRERFLDNEIDGTHLPSLTKDDYLDLGVTRVGHRMNIERALRRVMDREHLEWATA

Target ORF information:

RefSeq Version	XM_021471435.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471435.1

ORF Insert Sequence:

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGTG 
GACAGAGCCT CTGCCCCATG GAAAAAAAAA TCAGAGTTCG AATCTTCACA AGCTACAGAG 
AAGAAGAGGA CAGTTTATCA GATGGCTTTG AATAAACTGG ATGAGATTTT AGCAGCAGCA 
CAGCAGACCA TTAATACAAA TGAGGCTCCA GGGCCCCGGG TTCAGGGGGT AAAAAGAGAA 
CGGGGCAGAG GCTTTTATAA TGAGCCAAAT TTCGACCAAA CTGGAATGGG GATGACATCA 
TCTGGGGCAG GGTTAGGCTA CGACCGTGGT CAATTTGTGT CTGGCCATGG CCCCCCGCAC 
GGTATGCTGC GACAGAAGTC TATCGGGGTT CCTGAGGAGG AGAAGCAGTT TCTTCATCCT 
CCAGCCATGA AGTTTGCCCG TAGTCTTTCA GTACCTGGCC CAGATGAAAT CCCTCCTCCA 
CCTACTACAG CACCCCCTGA GCCCCCTTTT TCCTCAGCTC CTCCGCTTGG CTTTAGGGGC 
AAGACATCTC AACAGCCGTC TGTTTCTATG TCTCAGTCTA CTTCCTCTGC TGCACATTAT 
CAACTCTACT CACAGTCAGT ACATGTTACT CACGGACCAA GGGACAGGTC CCCTGGACCA 
CCTACTGGGT CTGGAACTGG GGACCTTGCT TATTCCCATG CCCAAGCCCA CCCCTCTGTA 
CCAAGTAGGA CTGCCTCCAG GAAGGTGATT GGGTACACAG GAGATCTTGC AGGGGCTGGA 
GGAGTGGCTG GGGCCAAAGC TGCAGCATTG AGAAGGGGTT ACAGCAATGC AGTCCCACCC 
ACCAGTGTCG CTACTGTGAT TCCACAACAG CAGCAGACTA CTCAGAACCA ACCTTCCAGA 
ATGGACCGGA GTGGAATGGG GGTAGGGGCT GGTGGTGTAC CAAAAGGCGG TGCTCGTAGA 
GGAAAGGGGC CCTTGGTGAA ACAGTCAAAG GTGGAAGACC TGCGTCAAGG TCAAGACACT 
CTAGAAGCAA AGGTGTCAGT GGAGAAGAGC TCTATTCCAA TCCCAACTAT CATTGTTAAA 
GCCCCTTCCA CCAGCAGTAG TGGACGCAGT AGCCAAGGCA GCAGTGTGGA GGCTGAACCA 
CCGCCCCCAG GAGAAAGTGA CAGTTCTAAA CCAGAACCTG TTGTTCCCCC ACCTGCACCA 
ACCGTGCCAC CACCAGCCCC TCCATCTATA CCTGCTCCTC CACCCCCATC ACTGCCATCA 
CTTCGTACTC AAGAGAGTCT GGACTTCACC AGCCAGTTTG GTGCTGCCAT TGTAGGGGCA 
GCTCGCAGGG ACAGAGAACG ATTTCATGAA GCACGACGCA AGAGTGCCTC CTTTTTTCTT 
TCTGCTGAGG AAGAGTTGAG TGGAGCTGGA GGAGCAGGAG GGCGGACTCA GACTTCTCAA 
CAGCAGCTGA GTTCCCAGCC TTCACCACGC CTGCGACCAT CCAAATCTAT AGATGAAGGC 
ATGTTCTCAG GTGATACTTT TATCCATCAC ACACGCAGTA TGCCTCCTGC ATTTGGCTTG 
CCTGAGTACT CCTCACCTGC TCCTCAAGGG GATTACCAGC CTAAGTCTGT ACCTGCTGAC 
TTCTATGGGG CAGGATCCAG ACAACAGCAG CAGCAGCAGC AGCAGCAAGC CCCCACCACA 
ACTTTTATTC ACCCACTAAC TGGAAAAGTA CTGGACCCTT CATCCCCTCT AGGCTTGGCT 
TTGGCTGCAC GGGAACGTGC CTTGAAGGAT GATGGACGTA TACGTAGGGA AAGGACCACT 
GAACATCATT TTACCCGTCA GTTGTCAAGC ATTGGGGCCT TTTCCTCATC CACTTCACAG 
CCAACATCAC ACATTTCCTA CCCAATGGCC TCGTCTTCCT CTGCAACCTC CTCTGCTGCT 
CAATCCACAA ACACCTCTTC ATATCTTGTT ACCTCCTCAT CATTAGCTGC CACCACCACA 
TCCACTCGGC CTCCATCTCC TAGGATCCTG CGTGGAGCAG GGAGTAGCTG GAGTGAAGAA 
GCTGGGGTTG GAGAGCGTGC GGATAGGGAA GCAGCTGGCA CCTCTGCTCC CAGAGAAGGC 
CTAAGGGTTC GTTTCTCCGA GGATAAAACT GTCCACACAC ACCACTATCA ATCTCAGCAA 
TATCAGCCCA CCTTTAAAGA GCGTGAAAGA GAACGTCAAA GAGAGAGGTC AAGAGAAAGA 
GAGAGGGAAA GAGAGAGGGA AAGAGAAATA GAAAAAGCTC AAACCCAGTA TCAGCAACAG 
TCATCACAAC CAATGACCCA GCAGCCTCGA CGCCCCTCAT TCTTGAGGAT GGAGAGTGAA 
GCTGGAGCGT ACATTCTTTC ATTAACGCCT CCATCTCCTC CTCCAACAGC AGCCCCTATG 
CGTCCTACAG CAGGAGCCAC AGGTGAAAGT GGAAGTGGTA CAAGCCTGAT GGTTCTGCCT 
CCCCCAGCTC CCTCAGTAGA TGCAGATGAT GAGTTTGTGT TTGCTGATCC TCTTCCTCCA 
CCTCTAGAGT TTGCCAATAG TTTTGATCGT GGTTTGGGTT ACTCTGCGAT GATTTCTCAC 
AATGTTGCAG CCCGTCAGCA GCGTCATCCT CCACCACCCC CTCCCCCACC ACCCCCGCCC 
ACTTCGAAAG ATTCCTTTAT GACAGGCTTT CCAGCTCACA CACCCCTACC GTCACCTCAG 
GCAGGAGATT CCACAACTTC CAGTCTTACC TCCTATGACA GTGAGGTTGC AAACCTAACA 
CAATCTGCCC CATCGCCCTC CCCATCTTCT CCCAACCCAC TACCACCTCA CTCCCCCTCC 
ACCCTTCAGA CATCTGGACC ACCTCCACCT CCCTCTGTAT CACCTCCTCC ACCACCCAAC 
AGCTACCATA GACCTTTTGG CTTATCTCAG TCACAACATT TATACAACAG TACACACACA 
ACTGGACGCT CCTCCTCTCC CACCCCACCT CTCTCTATTC CAGCTCCACC TGCCTACCGG 
GGGCCACCAA CAGCATCATC CACCCCTGCA ACTTCTGTAT CAATCAGAGG TGCCTCAACT 
GTGACCGCAA GCTGTTCCAC AACTGCTTCT ACAACTGTCA CCTCTCCTAG TACTACTGCT 
GCTGCCACAC CACACACACG CATAGCAACT GTCTCCACAG TAGCTACAGG GGGTAGTAGG 
CGTCCCAGTG CAGAGCATCA TCCACCACCT AGTGCAACCA AGAGTGGTGA GTCCCAGGAG 
ACAGTGGTAG ATTCTGGGAT TGAGGAGCTG GACAGCCGAA GCAGCAGTGA TCATCACTTA 
GACAGCATTC TTGCACTCAG TGGTAGTGGG GTCAGAGAGA GGTTGGAACG AGCAGGGATG 
AGTAGCATAG GTGGAATTGG AGGGAGAGAT GGTGCAACTG AGGTTGACAG GACAAGTGGG 
GCAGACTTTT TAGAGTCTTA CATGAGCTAC CTTGATGGGC AGACTTTTGA AATGCACAAT 
GCCAAAGCCG CTAGCACTGC ATCCACCTAC CCAAAAACAC AGAGGTACAG AGAAGGCACC 
CGGGCTGGAG ATCTTCGACG CCAGACCAGC ACAGCACCTC CTGCATACCA CCGTCGTAGA 
GAGGAGGAAG AGGAAGAGGA TGAGGCCGAG GAGGTAGAGG AGGAGGATTT GAGTGCCCGT 
GATGGCTATG GGATGAGGGA AGGGCCAGGG ATTGCTCGCT CCTCTGTAAT GTACTTTGAC 
CGGCCCCGCA CTCCAGAGAT AAAACCACTG TGGGGAGATG GGTCGACTGG AGCATCACAG 
ACCATGGGGG AGGGAGGGGG CCAGATAGTG GGAACATATG TGGAGCCACG TAAACTACAT 
CCTCCCATGA CTGGTATGAA AGCAAACATC ATTAATGAAC TAAGCACCAA ATTACAGCAG 
AGAAGTAAAG GAACAGAGAA CTGGGGGGCC CAGAGATCAT TGAGCAGACA TAGACACAGG 
TTCTCAGAGG ACTCAACAGG TGCACTGAGT CCTCCCTCTG TCCGCTCTAG CTCCCCATCT 
CCAGTTCAGC TCTCTCAGCC TGCCTTGTCT CCCTCTCCCA CTCCTTCCTC CCAGCCACTT 
TACTCAAACT GGGCTCGATC TCCTTCCCCT CAGCCCCCTG CTGCCTCATC CCAGCCCCCT 
CCCCGATCCC ATTCACCTGT TTCCCCTTCA TCATATTCAC CATACCCCAC CTCACCCAAA 
CACCGGCCTG TTTATCGCAC CAAAGCTCTG GAGTTCCAAT TTATTCCCAG TCGAGAGTCA 
CGAAAGTCAG AGTCGCATCA TGCCCAGCGC AGACGAGCAC CCAGCCCTTT AATTTCTCCA 
TCTGACCGTC CCAAGCTTGG CCCTCCCCGC CCCTCTTCCC TTCCTATTCT CCCCACTACA 
CCCCTCTATA GCAGCCTGTA TGACCTTCGT GGTTCTATTA CTCCACCCTC ACCTGCCAAT 
CCCCTTGGGG ACCCCTACTT TTCCCCCTCC CCCCCTCTAT TTGCCCCCTC GGGTGCTCCC 
CCTCCTCCAA ATTCCTCTCT GGTGGTCTCA CGGTCCCTGT CACCCACTCA CTTCCTGTCA 
GGGACTTCAT CCCCACCTCT CCACCCCATT CCTCCACCCA CTTGCCTGAG TTACCCCCAT 
CTTCCACCCC CCTCACCCAC AAAGCCCTTT GCCTCAAAAC CTCTCCCATA CTGGACCAAG 
TATGATGTTG CAGATTGGCT GGGCTACCTG AACCTGGGTG AACACAGGGA GCGATTCTTA 
GACAATGAAA TTGATGGCAC GCACCTGCCC TCTCTTACCA AGGATGACTA CCTGGACCTG 
GGTGTGACCC GAGTTGGCCA TCGCATGAAC ATAGAGAGGG CGCTCAGGAG AGTTATGGAC 
AGAGAACACT TGGAGTGGGC CACTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_021474082.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329757.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X8, mRNA.

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CloneID	ODa141770
Clone ID Related Accession (Same CDS sequence)	XM_021474082.1 , XM_021471438.1
Accession Version	XM_021474082.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGAA 
GTTCCACACT GTCACTACCA GCACCCCCAC TACAGTGGAC AGAGCCTCTG CCCCATGGAA 
AAAAAAATCA GAGTTCGAAT CTTCACAAGC TACAGAGAAG AAGAGGACAG TTTATCAGAT 
GGCTTTGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329757.1
CDS	393..3044
Translation	MTMPLSPLSSDEEQQRMLGNSQHLYPGAEEEEEEEEEMDDDGMDEEGEEENGELEMDDEEEVDDEDDIRRGPGHLRGGREEGHRQKGTMAGRLPGDRPIRPGNPGHAANPYSSNPGPTHQTSTNQQQQQPQRKLRERSSSNAPDDTHVALMVFRIGIPDIKQTKCLRFNPDATVWKAKQQVMCSLTESLRDVLNYGLFQPATDGHDAKFLEEERPLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKASLKKFMDYIQTSAVEKVAKLIDKGLDPNYHDPDTGETPLTLAVQTEPGGSEIIRVLVMGGAHIDFRARDGLTPLHKAVRAHAHTALLTLLSLGASSDYKDSRGLTPLYHTVLIGGDTSCCETLLYHRARLGLRDENGWDETHQACQNGNSQHLEHLLFYGADSSSQNASGNTALHICALYNKESCARILLYRGAKKDTKNNSGQNSFQVAVMSGHFELGEIIKNHRESDVVPFLESPKYAPQRRESSRTLGLPHPHPFLRANSDNSMNVPDWIAFPNAAGSSIVSVQGLKHGGTLRSSSSPRGARTRSPSRGRTGDRDDRSRQTRGRQGAGSISSQGTASGQRRRLYSAVPGRVFVATRSHSAQGDREISFNKGDKVKVLSVGEGGYWEGTVRGRTGWFPSDCVEEVALRSLESRSESRSERAKRLFRHYTVGSYDSFDAPSDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMEVPHCHYQHPHYSGQSLCPMEKKIRVRIFTSYREEEDSLSDGFE

Target ORF information:

RefSeq Version	XM_021474082.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474082.1

ORF Insert Sequence:

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGAA 
GTTCCACACT GTCACTACCA GCACCCCCAC TACAGTGGAC AGAGCCTCTG CCCCATGGAA 
AAAAAAATCA GAGTTCGAAT CTTCACAAGC TACAGAGAAG AAGAGGACAG TTTATCAGAT 
GGCTTTGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_021471438.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327113.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ODa141770
Clone ID Related Accession (Same CDS sequence)	XM_021474082.1 , XM_021471438.1
Accession Version	XM_021471438.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394533.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGAA 
GTTCCACACT GTCACTACCA GCACCCCCAC TACAGTGGAC AGAGCCTCTG CCCCATGGAA 
AAAAAAATCA GAGTTCGAAT CTTCACAAGC TACAGAGAAG AAGAGGACAG TTTATCAGAT 
GGCTTTGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327113.1
CDS	393..3044
Translation	MTMPLSPLSSDEEQQRMLGNSQHLYPGAEEEEEEEEEMDDDGMDEEGEEENGELEMDDEEEVDDEDDIRRGPGHLRGGREEGHRQKGTMAGRLPGDRPIRPGNPGHAANPYSSNPGPTHQTSTNQQQQQPQRKLRERSSSNAPDDTHVALMVFRIGIPDIKQTKCLRFNPDATVWKAKQQVMCSLTESLRDVLNYGLFQPATDGHDAKFLEEERPLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKASLKKFMDYIQTSAVEKVAKLIDKGLDPNYHDPDTGETPLTLAVQTEPGGSEIIRVLVMGGAHIDFRARDGLTPLHKAVRAHAHTALLTLLSLGASSDYKDSRGLTPLYHTVLIGGDTSCCETLLYHRARLGLRDENGWDETHQACQNGNSQHLEHLLFYGADSSSQNASGNTALHICALYNKESCARILLYRGAKKDTKNNSGQNSFQVAVMSGHFELGEIIKNHRESDVVPFLESPKYAPQRRESSRTLGLPHPHPFLRANSDNSMNVPDWIAFPNAAGSSIVSVQGLKHGGTLRSSSSPRGARTRSPSRGRTGDRDDRSRQTRGRQGAGSISSQGTASGQRRRLYSAVPGRVFVATRSHSAQGDREISFNKGDKVKVLSVGEGGYWEGTVRGRTGWFPSDCVEEVALRSLESRSESRSERAKRLFRHYTVGSYDSFDAPSDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMEVPHCHYQHPHYSGQSLCPMEKKIRVRIFTSYREEEDSLSDGFE

Target ORF information:

RefSeq Version	XM_021471438.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471438.1

ORF Insert Sequence:

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGAA 
GTTCCACACT GTCACTACCA GCACCCCCAC TACAGTGGAC AGAGCCTCTG CCCCATGGAA 
AAAAAAATCA GAGTTCGAAT CTTCACAAGC TACAGAGAAG AAGAGGACAG TTTATCAGAT 
GGCTTTGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_021471439.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327114.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ODa141771
Clone ID Related Accession (Same CDS sequence)	XM_021471439.1 , XM_021474085.1
Accession Version	XM_021471439.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2649bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394533.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGTT 
CCACACTGTC ACTACCAGCA CCCCCACTAC AGTGGACAGA GCCTCTGCCC CATGGAAAAA 
AAAATCAGAG TTCGAATCTT CACAAGCTAC AGAGAAGAAG AGGACAGTTT ATCAGATGGC 
TTTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327114.1
CDS	393..3041
Translation	MTMPLSPLSSDEEQQRMLGNSQHLYPGAEEEEEEEEEMDDDGMDEEGEEENGELEMDDEEEVDDEDDIRRGPGHLRGGREEGHRQKGTMAGRLPGDRPIRPGNPGHAANPYSSNPGPTHQTSTNQQQQQPQRKLRERSSSNAPDDTHVALMVFRIGIPDIKQTKCLRFNPDATVWKAKQQVMCSLTESLRDVLNYGLFQPATDGHDAKFLEEERPLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKASLKKFMDYIQTSAVEKVAKLIDKGLDPNYHDPDTGETPLTLAVQTEPGGSEIIRVLVMGGAHIDFRARDGLTPLHKAVRAHAHTALLTLLSLGASSDYKDSRGLTPLYHTVLIGGDTSCCETLLYHRARLGLRDENGWDETHQACQNGNSQHLEHLLFYGADSSSQNASGNTALHICALYNKESCARILLYRGAKKDTKNNSGQNSFQVAVMSGHFELGEIIKNHRESDVVPFLESPKYAPQRRESSRTLGLPHPHPFLRANSDNSMNVPDWIAFPNAAGSSIVSVQGLKHGGTLRSSSSPRGARTRSPSRGRTGDRDDRSRQTRGRQGAGSISSQGTASGQRRRLYSAVPGRVFVATRSHSAQGDREISFNKGDKVKVLSVGEGGYWEGTVRGRTGWFPSDCVEEVALRSLESRSESRSERAKRLFRHYTVGSYDSFDAPSDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMVPHCHYQHPHYSGQSLCPMEKKIRVRIFTSYREEEDSLSDGFE

Target ORF information:

RefSeq Version	XM_021471439.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471439.1

ORF Insert Sequence:

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGTT 
CCACACTGTC ACTACCAGCA CCCCCACTAC AGTGGACAGA GCCTCTGCCC CATGGAAAAA 
AAAATCAGAG TTCGAATCTT CACAAGCTAC AGAGAAGAAG AGGACAGTTT ATCAGATGGC 
TTTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_021474085.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329760.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X9, mRNA.

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CloneID	ODa141771
Clone ID Related Accession (Same CDS sequence)	XM_021471439.1 , XM_021474085.1
Accession Version	XM_021474085.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2649bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGTT 
CCACACTGTC ACTACCAGCA CCCCCACTAC AGTGGACAGA GCCTCTGCCC CATGGAAAAA 
AAAATCAGAG TTCGAATCTT CACAAGCTAC AGAGAAGAAG AGGACAGTTT ATCAGATGGC 
TTTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329760.1
CDS	393..3041
Translation	MTMPLSPLSSDEEQQRMLGNSQHLYPGAEEEEEEEEEMDDDGMDEEGEEENGELEMDDEEEVDDEDDIRRGPGHLRGGREEGHRQKGTMAGRLPGDRPIRPGNPGHAANPYSSNPGPTHQTSTNQQQQQPQRKLRERSSSNAPDDTHVALMVFRIGIPDIKQTKCLRFNPDATVWKAKQQVMCSLTESLRDVLNYGLFQPATDGHDAKFLEEERPLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKASLKKFMDYIQTSAVEKVAKLIDKGLDPNYHDPDTGETPLTLAVQTEPGGSEIIRVLVMGGAHIDFRARDGLTPLHKAVRAHAHTALLTLLSLGASSDYKDSRGLTPLYHTVLIGGDTSCCETLLYHRARLGLRDENGWDETHQACQNGNSQHLEHLLFYGADSSSQNASGNTALHICALYNKESCARILLYRGAKKDTKNNSGQNSFQVAVMSGHFELGEIIKNHRESDVVPFLESPKYAPQRRESSRTLGLPHPHPFLRANSDNSMNVPDWIAFPNAAGSSIVSVQGLKHGGTLRSSSSPRGARTRSPSRGRTGDRDDRSRQTRGRQGAGSISSQGTASGQRRRLYSAVPGRVFVATRSHSAQGDREISFNKGDKVKVLSVGEGGYWEGTVRGRTGWFPSDCVEEVALRSLESRSESRSERAKRLFRHYTVGSYDSFDAPSDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMVPHCHYQHPHYSGQSLCPMEKKIRVRIFTSYREEEDSLSDGFE

Target ORF information:

RefSeq Version	XM_021474085.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474085.1

ORF Insert Sequence:

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGTT 
CCACACTGTC ACTACCAGCA CCCCCACTAC AGTGGACAGA GCCTCTGCCC CATGGAAAAA 
AAAATCAGAG TTCGAATCTT CACAAGCTAC AGAGAAGAAG AGGACAGTTT ATCAGATGGC 
TTTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_009299485.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297760.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa45591
Clone ID Related Accession (Same CDS sequence)	XM_009299485.2 , XM_009299487.2 , XM_009299484.2
Accession Version	XM_009299485.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299485.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGGTGTGGA TAAACGTTGT 
CTGTTGCTGA GTGTGGGTGA AGGAGGCTAT TGGGAAGGGA CGGTTCGAGG ACGCACAGGC 
TGGTTCCCCT CAGACTGCGT GGAGGAAGTT GCTCTCCGTA GCCTGGAATC TCGTTCAGAG 
AGCCGCAGTG AGAGAGCCAA GAGACTCTTC CGGCATTATA CTGTTGGATC CTACGACAGC 
TTCGATGCGC CCAGTGACTA CATAATCAAG GAGAAGACAG TCCTCCTGCA GAAAAAAGAC 
AATGAAGGTT TTGGCTTTGT GCTGAGAGGA GCCAAGGCTC AGACCCCTAT AGAAGAGTTC 
ACTCCCACCC CAGCCTTTCC AGCACTGCAG TACCTTGAGT CTGTCGACGA GGGAGGCGTG 
GCCTGGAGAG CTGGTCTGCG AATGGGAGAC TTCCTTATAG AGGTGAATGG TCAGAATGTG 
GTTAAGGTGG GACACAGGCA GGTGGTCAAT ATGATCAGGC AAGGCGGTAA CAGTCTCATG 
GTCAAGGTTG TCATGGTTAC TCGAAATCCT GATATGGAGG AAGGGGTCAG AAAGAAAATC 
CCACAGCAGA GTAAAAGACT GAGCACACCG GCTATCGCTC TGCGCTCCAA ATCCATGACA 
TCAGAGCTGG AAGAGATGGT GGACAGAGCC TCTGCCCCAT GGAAAAAAAA ATCAGAGTTC 
GAATCTTCAC AAGCTACAGA GAAGAAGAGG ACAGTTTATC AGATGGCTTT GAATAAACTG 
GATGAGATTT TAGCAGCAGC ACAGCAGACC ATTAATACAA ATGAGGCTCC AGGGCCCCGG 
GTTCAGGGGG TAAAAAGAGA ACGGGGCAGA GGCTTTTATA ATGAGCCAAA TTTCGACCAA 
ACTGGAATGG GGATGACATC ATCTGGGGCA GGGTTAGGCT ACGACCGTGG TCAATTTGTG 
TCTGGCCATG GCCCCCCGCA CGGTATGCTG CGACAGAAGT CTATCGGGGT TCCTGAGGAG 
GAGAAGCAGT TTCTTCATCC TCCAGCCATG AAGTTTGCCC GTAGTCTTTC AGTACCTGGC 
CCAGATGAAA TCCCTCCTCC ACCTACTACA GCACCCCCTG AGCCCCCTTT TTCCTCAGCT 
CCTCCGCTTG GCTTTAGGGG CAAGACATCT CAACAGCCGT CTGTTTCTAT GTCTCAGTCT 
ACTTCCTCTG CTGCACATTA TCAACTCTAC TCACAGTCAG TACATGTTAC TCACGGACCA 
AGGGACAGGT CCCCTGGACC ACCTACTGGG TCTGGAACTG GGGACCTTGC TTATTCCCAT 
GCCCAAGCCC ACCCCTCTGT ACCAAGTAGG ACTGCCTCCA GGAAGGTGAT TGGGTACACA 
GGAGATCTTG CAGGGGCTGG AGGAGTGGCT GGGGCCAAAG CTGCAGCATT GAGAAGGGGT 
TACAGCAATG CAGTCCCACC CACCAGTGTC GCTACTGTGA TTCCACAACA GCAGCAGACT 
ACTCAGAACC AACCTTCCAG AATGGACCGG AGTGGAATGG GGGTAGGGGC TGGTGGTGTA 
CCAAAAGGCG GTGCTCGTAG AGGAAAGGGG CCCTTGGTGA AACAGTCAAA GGTGGAAGAC 
CTGCGTCAAG GTCAAGACAC TCTAGAAGCA AAGGTGTCAG TGGAGAAGAG CTCTATTCCA 
ATCCCAACTA TCATTGTTAA AGCCCCTTCC ACCAGCAGTA GTGGACGCAG TAGCCAAGGC 
AGCAGTGTGG AGGCTGAACC ACCGCCCCCA GGAGAAAGTG ACAGTTCTAA ACCAGAACCT 
GTTGTTCCCC CACCTGCACC AACCGTGCCA CCACCAGCCC CTCCATCTAT ACCTGCTCCT 
CCACCCCCAT CACTGCCATC ACTTCGTACT CAAGAGAGTC TGGACTTCAC CAGCCAGTTT 
GGTGCTGCCA TTGTAGGGGC AGCTCGCAGG GACAGAGAAC GATTTCATGA AGCACGACGC 
AAGAGTGCCT CCTTTTTTCT TTCTGCTGAG GAAGAGTTGA GTGGAGCTGG AGGAGCAGGA 
GGGCGGACTC AGACTTCTCA ACAGCAGCTG AGTTCCCAGC CTTCACCACG CCTGCGACCA 
TCCAAATCTA TAGATGAAGG CATGTTCTCA GGTGATACTT TTATCCATCA CACACGCAGT 
ATGCCTCCTG CATTTGGCTT GCCTGAGTAC TCCTCACCTG CTCCTCAAGG GGATTACCAG 
CCTAAGTCTG TACCTGCTGA CTTCTATGGG GCAGGATCCA GACAACAGCA GCAGCAGCAG 
CAGCAGCAAG CCCCCACCAC AACTTTTATT CACCCACTAA CTGGAAAAGT ACTGGACCCT 
TCATCCCCTC TAGGCTTGGC TTTGGCTGCA CGGGAACGTG CCTTGAAGGA TGATGGACGT 
ATACGTAGGG AAAGGACCAC TGAACATCAT TTTACCCGTC AGTTGTCAAG CATTGGGGCC 
TTTTCCTCAT CCACTTCACA GCCAACATCA CACATTTCCT ACCCAATGGC CTCGTCTTCC 
TCTGCAACCT CCTCTGCTGC TCAATCCACA AACACCTCTT CATATCTTGT TACCTCCTCA 
TCATTAGCTG CCACCACCAC ATCCACTCGG CCTCCATCTC CTAGGATCCT GCGTGGAGCA 
GGGAGTAGCT GGAGTGAAGA AGCTGGGGTT GGAGAGCGTG CGGATAGGGA AGCAGCTGGC 
ACCTCTGCTC CCAGAGAAGG CCTAAGGGTT CGTTTCTCCG AGGATAAAAC TGTCCACACA 
CACCACTATC AATCTCAGCA ATATCAGCCC ACCTTTAAAG AGCGTGAAAG AGAACGTCAA 
AGAGAGAGGT CAAGAGAAAG AGAGAGGGAA AGAGAGAGGG AAAGAGAAAT AGAAAAAGCT 
CAAACCCAGT ATCAGCAACA GTCATCACAA CCAATGACCC AGCAGCCTCG ACGCCCCTCA 
TTCTTGAGGA TGGAGAGTGA AGCTGGAGCG TACATTCTTT CATTAACGCC TCCATCTCCT 
CCTCCAACAG CAGCCCCTAT GCGTCCTACA GCAGGAGCCA CAGGTGAAAG TGGAAGTGGT 
ACAAGCCTGA TGGTTCTGCC TCCCCCAGCT CCCTCAGTAG ATGCAGATGA TGAGTTTGTG 
TTTGCTGATC CTCTTCCTCC ACCTCTAGAG TTTGCCAATA GTTTTGATCG TGGTTTGGGT 
TACTCTGCGA TGATTTCTCA CAATGTTGCA GCCCGTCAGC AGCGTCATCC TCCACCACCC 
CCTCCCCCAC CACCCCCGCC CACTTCGAAA GATTCCTTTA TGACAGGCTT TCCAGCTCAC 
ACACCCCTAC CGTCACCTCA GGCAGGAGAT TCCACAACTT CCAGTCTTAC CTCCTATGAC 
AGTGAGGTTG CAAACCTAAC ACAATCTGCC CCATCGCCCT CCCCATCTTC TCCCAACCCA 
CTACCACCTC ACTCCCCCTC CACCCTTCAG ACATCTGGAC CACCTCCACC TCCCTCTGTA 
TCACCTCCTC CACCACCCAA CAGCTACCAT AGACCTTTTG GCTTATCTCA GTCACAACAT 
TTATACAACA GTACACACAC AACTGGACGC TCCTCCTCTC CCACCCCACC TCTCTCTATT 
CCAGCTCCAC CTGCCTACCG GGGGCCACCA ACAGCATCAT CCACCCCTGC AACTTCTGTA 
TCAATCAGAG GTGCCTCAAC TGTGACCGCA AGCTGTTCCA CAACTGCTTC TACAACTGTC 
ACCTCTCCTA GTACTACTGC TGCTGCCACA CCACACACAC GCATAGCAAC TGTCTCCACA 
GTAGCTACAG GGGGTAGTAG GCGTCCCAGT GCAGAGCATC ATCCACCACC TAGTGCAACC 
AAGAGTGGTG AGTCCCAGGA GACAGTGGTA GATTCTGGGA TTGAGGAGCT GGACAGCCGA 
AGCAGCAGTG ATCATCACTT AGACAGCATT CTTGCACTCA GTGGTAGTGG GGTCAGAGAG 
AGGTTGGAAC GAGCAGGGAT GAGTAGCATA GGTGGAATTG GAGGGAGAGA TGGTGCAACT 
GAGGTTGACA GGACAAGTGG GGCAGACTTT TTAGAGTCTT ACATGAGCTA CCTTGATGGG 
CAGACTTTTG AAATGCACAA TGCCAAAGCC GCTAGCACTG CATCCACCTA CCCAAAAACA 
CAGAGGTACA GAGAAGGCAC CCGGGCTGGA GATCTTCGAC GCCAGACCAG CACAGCACCT 
CCTGCATACC ACCGTCGTAG AGAGGAGGAA GAGGAAGAGG ATGAGGCCGA GGAGGTAGAG 
GAGGAGGATT TGAGTGCCCG TGATGGCTAT GGGATGAGGG AAGGGCCAGG GATTGCTCGC 
TCCTCTGTAA TGTACTTTGA CCGGCCCCGC ACTCCAGAGA TAAAACCACT GTGGGGAGAT 
GGGTCGACTG GAGCATCACA GACCATGGGG GAGGGAGGGG GCCAGATAGT GGGAACATAT 
GTGGAGCCAC GTAAACTACA TCCTCCCATG ACTGGTATGA AAGCAAACAT CATTAATGAA 
CTAAGCACCA AATTACAGCA GAGAAGTAAA GGAACAGAGA ACTGGGGGGC CCAGAGATCA 
TTGAGCAGAC ATAGACACAG GTTCTCAGAG GACTCAACAG GTGCACTGAG TCCTCCCTCT 
GTCCGCTCTA GCTCCCCATC TCCAGTTCAG CTCTCTCAGC CTGCCTTGTC TCCCTCTCCC 
ACTCCTTCCT CCCAGCCACT TTACTCAAAC TGGGCTCGAT CTCCTTCCCC TCAGCCCCCT 
GCTGCCTCAT CCCAGCCCCC TCCCCGATCC CATTCACCTG TTTCCCCTTC ATCATATTCA 
CCATACCCCA CCTCACCCAA ACACCGGCCT GTTTATCGCA CCAAAGCTCT GGAGTTCCAA 
TTTATTCCCA GTCGAGAGTC ACGAAAGTCA GAGTCGCATC ATGCCCAGCG CAGACGAGCA 
CCCAGCCCTT TAATTTCTCC ATCTGACCGT CCCAAGCTTG GCCCTCCCCG CCCCTCTTCC 
CTTCCTATTC TCCCCACTAC ACCCCTCTAT AGCAGCCTGT ATGACCTTCG TGGTTCTATT 
ACTCCACCCT CACCTGCCAA TCCCCTTGGG GACCCCTACT TTTCCCCCTC CCCCCCTCTA 
TTTGCCCCCT CGGGTGCTCC CCCTCCTCCA AATTCCTCTC TGGTGGTCTC ACGGTCCCTG 
TCACCCACTC ACTTCCTGTC AGGGACTTCA TCCCCACCTC TCCACCCCAT TCCTCCACCC 
ACTTGCCTGA GTTACCCCCA TCTTCCACCC CCCTCACCCA CAAAGCCCTT TGCCTCAAAA 
CCTCTCCCAT ACTGGACCAA GTATGATGTT GCAGATTGGC TGGGCTACCT GAACCTGGGT 
GAACACAGGG AGCGATTCTT AGACAATGAA ATTGATGGCA CGCACCTGCC CTCTCTTACC 
AAGGATGACT ACCTGGACCT GGGTGTGACC CGAGTTGGCC ATCGCATGAA CATAGAGAGG 
GCGCTCAGGA GAGTTATGGA CAGAACCATC AGTAAAGATC TCATCCCCTC CATCTCCATA 
TCTTTCATCT GTTCATCTTC TAAGAGTGTG GCATCTTCAT TCATCATACT GCTCACTTTA 
CCTTACTCAT ACTGGACAAA AAGATTGAGG AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297760.1
CDS	576..8051
Translation	MTMPLSPLSSDEEQQRMLGNSQHLYPGAEEEEEEEEEMDDDGMDEEGEEENGELEMDDEEEVDDEDDIRRGPGHLRGGREEGHRQKGTMAGRLPGDRPIRPGNPGHAANPYSSNPGPTHQTSTNQQQQQPQRKLRERSSSNAPDDTHVALMVFRIGIPDIKQTKCLRFNPDATVWKAKQQVMCSLTESLRDVLNYGLFQPATDGHDAKFLEEERPLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKASLKKFMDYIQTSAVEKVAKLIDKGLDPNYHDPDTGETPLTLAVQTEPGGSEIIRVLVMGGAHIDFRARDGLTPLHKAVRAHAHTALLTLLSLGASSDYKDSRGLTPLYHTVLIGGDTSCCETLLYHRARLGLRDENGWDETHQACQNGNSQHLEHLLFYGADSSSQNASGNTALHICALYNKESCARILLYRGAKKDTKNNSGQNSFQVAVMSGHFELGEIIKNHRESDVVPFLESPKYAPQRRESSRTLGLPHPHPFLRANSDNSMNVPDWIAFPNAAGSSIVSVQGLKHGGTLRSSSSPRGARTRSPSRGRTGDRDDRSRQTRGRQGAGSISSQGTASGQRRRLYSAVPGRVFVATRSHSAQGDREISFNKGDKVKGVDKRCLLLSVGEGGYWEGTVRGRTGWFPSDCVEEVALRSLESRSESRSERAKRLFRHYTVGSYDSFDAPSDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMVDRASAPWKKKSEFESSQATEKKRTVYQMALNKLDEILAAAQQTINTNEAPGPRVQGVKRERGRGFYNEPNFDQTGMGMTSSGAGLGYDRGQFVSGHGPPHGMLRQKSIGVPEEEKQFLHPPAMKFARSLSVPGPDEIPPPPTTAPPEPPFSSAPPLGFRGKTSQQPSVSMSQSTSSAAHYQLYSQSVHVTHGPRDRSPGPPTGSGTGDLAYSHAQAHPSVPSRTASRKVIGYTGDLAGAGGVAGAKAAALRRGYSNAVPPTSVATVIPQQQQTTQNQPSRMDRSGMGVGAGGVPKGGARRGKGPLVKQSKVEDLRQGQDTLEAKVSVEKSSIPIPTIIVKAPSTSSSGRSSQGSSVEAEPPPPGESDSSKPEPVVPPPAPTVPPPAPPSIPAPPPPSLPSLRTQESLDFTSQFGAAIVGAARRDRERFHEARRKSASFFLSAEEELSGAGGAGGRTQTSQQQLSSQPSPRLRPSKSIDEGMFSGDTFIHHTRSMPPAFGLPEYSSPAPQGDYQPKSVPADFYGAGSRQQQQQQQQQAPTTTFIHPLTGKVLDPSSPLGLALAARERALKDDGRIRRERTTEHHFTRQLSSIGAFSSSTSQPTSHISYPMASSSSATSSAAQSTNTSSYLVTSSSLAATTTSTRPPSPRILRGAGSSWSEEAGVGERADREAAGTSAPREGLRVRFSEDKTVHTHHYQSQQYQPTFKERERERQRERSREREREREREREIEKAQTQYQQQSSQPMTQQPRRPSFLRMESEAGAYILSLTPPSPPPTAAPMRPTAGATGESGSGTSLMVLPPPAPSVDADDEFVFADPLPPPLEFANSFDRGLGYSAMISHNVAARQQRHPPPPPPPPPPPTSKDSFMTGFPAHTPLPSPQAGDSTTSSLTSYDSEVANLTQSAPSPSPSSPNPLPPHSPSTLQTSGPPPPPSVSPPPPPNSYHRPFGLSQSQHLYNSTHTTGRSSSPTPPLSIPAPPAYRGPPTASSTPATSVSIRGASTVTASCSTTASTTVTSPSTTAAATPHTRIATVSTVATGGSRRPSAEHHPPPSATKSGESQETVVDSGIEELDSRSSSDHHLDSILALSGSGVRERLERAGMSSIGGIGGRDGATEVDRTSGADFLESYMSYLDGQTFEMHNAKAASTASTYPKTQRYREGTRAGDLRRQTSTAPPAYHRRREEEEEEDEAEEVEEEDLSARDGYGMREGPGIARSSVMYFDRPRTPEIKPLWGDGSTGASQTMGEGGGQIVGTYVEPRKLHPPMTGMKANIINELSTKLQQRSKGTENWGAQRSLSRHRHRFSEDSTGALSPPSVRSSSPSPVQLSQPALSPSPTPSSQPLYSNWARSPSPQPPAASSQPPPRSHSPVSPSSYSPYPTSPKHRPVYRTKALEFQFIPSRESRKSESHHAQRRRAPSPLISPSDRPKLGPPRPSSLPILPTTPLYSSLYDLRGSITPPSPANPLGDPYFSPSPPLFAPSGAPPPPNSSLVVSRSLSPTHFLSGTSSPPLHPIPPPTCLSYPHLPPPSPTKPFASKPLPYWTKYDVADWLGYLNLGEHRERFLDNEIDGTHLPSLTKDDYLDLGVTRVGHRMNIERALRRVMDRTISKDLIPSISISFICSSSKSVASSFIILLTLPYSYWTKRLRN

Target ORF information:

RefSeq Version	XM_009299485.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299485.2

ORF Insert Sequence:

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGGTGTGGA TAAACGTTGT 
CTGTTGCTGA GTGTGGGTGA AGGAGGCTAT TGGGAAGGGA CGGTTCGAGG ACGCACAGGC 
TGGTTCCCCT CAGACTGCGT GGAGGAAGTT GCTCTCCGTA GCCTGGAATC TCGTTCAGAG 
AGCCGCAGTG AGAGAGCCAA GAGACTCTTC CGGCATTATA CTGTTGGATC CTACGACAGC 
TTCGATGCGC CCAGTGACTA CATAATCAAG GAGAAGACAG TCCTCCTGCA GAAAAAAGAC 
AATGAAGGTT TTGGCTTTGT GCTGAGAGGA GCCAAGGCTC AGACCCCTAT AGAAGAGTTC 
ACTCCCACCC CAGCCTTTCC AGCACTGCAG TACCTTGAGT CTGTCGACGA GGGAGGCGTG 
GCCTGGAGAG CTGGTCTGCG AATGGGAGAC TTCCTTATAG AGGTGAATGG TCAGAATGTG 
GTTAAGGTGG GACACAGGCA GGTGGTCAAT ATGATCAGGC AAGGCGGTAA CAGTCTCATG 
GTCAAGGTTG TCATGGTTAC TCGAAATCCT GATATGGAGG AAGGGGTCAG AAAGAAAATC 
CCACAGCAGA GTAAAAGACT GAGCACACCG GCTATCGCTC TGCGCTCCAA ATCCATGACA 
TCAGAGCTGG AAGAGATGGT GGACAGAGCC TCTGCCCCAT GGAAAAAAAA ATCAGAGTTC 
GAATCTTCAC AAGCTACAGA GAAGAAGAGG ACAGTTTATC AGATGGCTTT GAATAAACTG 
GATGAGATTT TAGCAGCAGC ACAGCAGACC ATTAATACAA ATGAGGCTCC AGGGCCCCGG 
GTTCAGGGGG TAAAAAGAGA ACGGGGCAGA GGCTTTTATA ATGAGCCAAA TTTCGACCAA 
ACTGGAATGG GGATGACATC ATCTGGGGCA GGGTTAGGCT ACGACCGTGG TCAATTTGTG 
TCTGGCCATG GCCCCCCGCA CGGTATGCTG CGACAGAAGT CTATCGGGGT TCCTGAGGAG 
GAGAAGCAGT TTCTTCATCC TCCAGCCATG AAGTTTGCCC GTAGTCTTTC AGTACCTGGC 
CCAGATGAAA TCCCTCCTCC ACCTACTACA GCACCCCCTG AGCCCCCTTT TTCCTCAGCT 
CCTCCGCTTG GCTTTAGGGG CAAGACATCT CAACAGCCGT CTGTTTCTAT GTCTCAGTCT 
ACTTCCTCTG CTGCACATTA TCAACTCTAC TCACAGTCAG TACATGTTAC TCACGGACCA 
AGGGACAGGT CCCCTGGACC ACCTACTGGG TCTGGAACTG GGGACCTTGC TTATTCCCAT 
GCCCAAGCCC ACCCCTCTGT ACCAAGTAGG ACTGCCTCCA GGAAGGTGAT TGGGTACACA 
GGAGATCTTG CAGGGGCTGG AGGAGTGGCT GGGGCCAAAG CTGCAGCATT GAGAAGGGGT 
TACAGCAATG CAGTCCCACC CACCAGTGTC GCTACTGTGA TTCCACAACA GCAGCAGACT 
ACTCAGAACC AACCTTCCAG AATGGACCGG AGTGGAATGG GGGTAGGGGC TGGTGGTGTA 
CCAAAAGGCG GTGCTCGTAG AGGAAAGGGG CCCTTGGTGA AACAGTCAAA GGTGGAAGAC 
CTGCGTCAAG GTCAAGACAC TCTAGAAGCA AAGGTGTCAG TGGAGAAGAG CTCTATTCCA 
ATCCCAACTA TCATTGTTAA AGCCCCTTCC ACCAGCAGTA GTGGACGCAG TAGCCAAGGC 
AGCAGTGTGG AGGCTGAACC ACCGCCCCCA GGAGAAAGTG ACAGTTCTAA ACCAGAACCT 
GTTGTTCCCC CACCTGCACC AACCGTGCCA CCACCAGCCC CTCCATCTAT ACCTGCTCCT 
CCACCCCCAT CACTGCCATC ACTTCGTACT CAAGAGAGTC TGGACTTCAC CAGCCAGTTT 
GGTGCTGCCA TTGTAGGGGC AGCTCGCAGG GACAGAGAAC GATTTCATGA AGCACGACGC 
AAGAGTGCCT CCTTTTTTCT TTCTGCTGAG GAAGAGTTGA GTGGAGCTGG AGGAGCAGGA 
GGGCGGACTC AGACTTCTCA ACAGCAGCTG AGTTCCCAGC CTTCACCACG CCTGCGACCA 
TCCAAATCTA TAGATGAAGG CATGTTCTCA GGTGATACTT TTATCCATCA CACACGCAGT 
ATGCCTCCTG CATTTGGCTT GCCTGAGTAC TCCTCACCTG CTCCTCAAGG GGATTACCAG 
CCTAAGTCTG TACCTGCTGA CTTCTATGGG GCAGGATCCA GACAACAGCA GCAGCAGCAG 
CAGCAGCAAG CCCCCACCAC AACTTTTATT CACCCACTAA CTGGAAAAGT ACTGGACCCT 
TCATCCCCTC TAGGCTTGGC TTTGGCTGCA CGGGAACGTG CCTTGAAGGA TGATGGACGT 
ATACGTAGGG AAAGGACCAC TGAACATCAT TTTACCCGTC AGTTGTCAAG CATTGGGGCC 
TTTTCCTCAT CCACTTCACA GCCAACATCA CACATTTCCT ACCCAATGGC CTCGTCTTCC 
TCTGCAACCT CCTCTGCTGC TCAATCCACA AACACCTCTT CATATCTTGT TACCTCCTCA 
TCATTAGCTG CCACCACCAC ATCCACTCGG CCTCCATCTC CTAGGATCCT GCGTGGAGCA 
GGGAGTAGCT GGAGTGAAGA AGCTGGGGTT GGAGAGCGTG CGGATAGGGA AGCAGCTGGC 
ACCTCTGCTC CCAGAGAAGG CCTAAGGGTT CGTTTCTCCG AGGATAAAAC TGTCCACACA 
CACCACTATC AATCTCAGCA ATATCAGCCC ACCTTTAAAG AGCGTGAAAG AGAACGTCAA 
AGAGAGAGGT CAAGAGAAAG AGAGAGGGAA AGAGAGAGGG AAAGAGAAAT AGAAAAAGCT 
CAAACCCAGT ATCAGCAACA GTCATCACAA CCAATGACCC AGCAGCCTCG ACGCCCCTCA 
TTCTTGAGGA TGGAGAGTGA AGCTGGAGCG TACATTCTTT CATTAACGCC TCCATCTCCT 
CCTCCAACAG CAGCCCCTAT GCGTCCTACA GCAGGAGCCA CAGGTGAAAG TGGAAGTGGT 
ACAAGCCTGA TGGTTCTGCC TCCCCCAGCT CCCTCAGTAG ATGCAGATGA TGAGTTTGTG 
TTTGCTGATC CTCTTCCTCC ACCTCTAGAG TTTGCCAATA GTTTTGATCG TGGTTTGGGT 
TACTCTGCGA TGATTTCTCA CAATGTTGCA GCCCGTCAGC AGCGTCATCC TCCACCACCC 
CCTCCCCCAC CACCCCCGCC CACTTCGAAA GATTCCTTTA TGACAGGCTT TCCAGCTCAC 
ACACCCCTAC CGTCACCTCA GGCAGGAGAT TCCACAACTT CCAGTCTTAC CTCCTATGAC 
AGTGAGGTTG CAAACCTAAC ACAATCTGCC CCATCGCCCT CCCCATCTTC TCCCAACCCA 
CTACCACCTC ACTCCCCCTC CACCCTTCAG ACATCTGGAC CACCTCCACC TCCCTCTGTA 
TCACCTCCTC CACCACCCAA CAGCTACCAT AGACCTTTTG GCTTATCTCA GTCACAACAT 
TTATACAACA GTACACACAC AACTGGACGC TCCTCCTCTC CCACCCCACC TCTCTCTATT 
CCAGCTCCAC CTGCCTACCG GGGGCCACCA ACAGCATCAT CCACCCCTGC AACTTCTGTA 
TCAATCAGAG GTGCCTCAAC TGTGACCGCA AGCTGTTCCA CAACTGCTTC TACAACTGTC 
ACCTCTCCTA GTACTACTGC TGCTGCCACA CCACACACAC GCATAGCAAC TGTCTCCACA 
GTAGCTACAG GGGGTAGTAG GCGTCCCAGT GCAGAGCATC ATCCACCACC TAGTGCAACC 
AAGAGTGGTG AGTCCCAGGA GACAGTGGTA GATTCTGGGA TTGAGGAGCT GGACAGCCGA 
AGCAGCAGTG ATCATCACTT AGACAGCATT CTTGCACTCA GTGGTAGTGG GGTCAGAGAG 
AGGTTGGAAC GAGCAGGGAT GAGTAGCATA GGTGGAATTG GAGGGAGAGA TGGTGCAACT 
GAGGTTGACA GGACAAGTGG GGCAGACTTT TTAGAGTCTT ACATGAGCTA CCTTGATGGG 
CAGACTTTTG AAATGCACAA TGCCAAAGCC GCTAGCACTG CATCCACCTA CCCAAAAACA 
CAGAGGTACA GAGAAGGCAC CCGGGCTGGA GATCTTCGAC GCCAGACCAG CACAGCACCT 
CCTGCATACC ACCGTCGTAG AGAGGAGGAA GAGGAAGAGG ATGAGGCCGA GGAGGTAGAG 
GAGGAGGATT TGAGTGCCCG TGATGGCTAT GGGATGAGGG AAGGGCCAGG GATTGCTCGC 
TCCTCTGTAA TGTACTTTGA CCGGCCCCGC ACTCCAGAGA TAAAACCACT GTGGGGAGAT 
GGGTCGACTG GAGCATCACA GACCATGGGG GAGGGAGGGG GCCAGATAGT GGGAACATAT 
GTGGAGCCAC GTAAACTACA TCCTCCCATG ACTGGTATGA AAGCAAACAT CATTAATGAA 
CTAAGCACCA AATTACAGCA GAGAAGTAAA GGAACAGAGA ACTGGGGGGC CCAGAGATCA 
TTGAGCAGAC ATAGACACAG GTTCTCAGAG GACTCAACAG GTGCACTGAG TCCTCCCTCT 
GTCCGCTCTA GCTCCCCATC TCCAGTTCAG CTCTCTCAGC CTGCCTTGTC TCCCTCTCCC 
ACTCCTTCCT CCCAGCCACT TTACTCAAAC TGGGCTCGAT CTCCTTCCCC TCAGCCCCCT 
GCTGCCTCAT CCCAGCCCCC TCCCCGATCC CATTCACCTG TTTCCCCTTC ATCATATTCA 
CCATACCCCA CCTCACCCAA ACACCGGCCT GTTTATCGCA CCAAAGCTCT GGAGTTCCAA 
TTTATTCCCA GTCGAGAGTC ACGAAAGTCA GAGTCGCATC ATGCCCAGCG CAGACGAGCA 
CCCAGCCCTT TAATTTCTCC ATCTGACCGT CCCAAGCTTG GCCCTCCCCG CCCCTCTTCC 
CTTCCTATTC TCCCCACTAC ACCCCTCTAT AGCAGCCTGT ATGACCTTCG TGGTTCTATT 
ACTCCACCCT CACCTGCCAA TCCCCTTGGG GACCCCTACT TTTCCCCCTC CCCCCCTCTA 
TTTGCCCCCT CGGGTGCTCC CCCTCCTCCA AATTCCTCTC TGGTGGTCTC ACGGTCCCTG 
TCACCCACTC ACTTCCTGTC AGGGACTTCA TCCCCACCTC TCCACCCCAT TCCTCCACCC 
ACTTGCCTGA GTTACCCCCA TCTTCCACCC CCCTCACCCA CAAAGCCCTT TGCCTCAAAA 
CCTCTCCCAT ACTGGACCAA GTATGATGTT GCAGATTGGC TGGGCTACCT GAACCTGGGT 
GAACACAGGG AGCGATTCTT AGACAATGAA ATTGATGGCA CGCACCTGCC CTCTCTTACC 
AAGGATGACT ACCTGGACCT GGGTGTGACC CGAGTTGGCC ATCGCATGAA CATAGAGAGG 
GCGCTCAGGA GAGTTATGGA CAGAACCATC AGTAAAGATC TCATCCCCTC CATCTCCATA 
TCTTTCATCT GTTCATCTTC TAAGAGTGTG GCATCTTCAT TCATCATACT GCTCACTTTA 
CCTTACTCAT ACTGGACAAA AAGATTGAGG AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_009299487.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297762.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X2, mRNA.

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CloneID	ODa45591
Clone ID Related Accession (Same CDS sequence)	XM_009299485.2 , XM_009299487.2 , XM_009299484.2
Accession Version	XM_009299487.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299487.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGGTGTGGA TAAACGTTGT 
CTGTTGCTGA GTGTGGGTGA AGGAGGCTAT TGGGAAGGGA CGGTTCGAGG ACGCACAGGC 
TGGTTCCCCT CAGACTGCGT GGAGGAAGTT GCTCTCCGTA GCCTGGAATC TCGTTCAGAG 
AGCCGCAGTG AGAGAGCCAA GAGACTCTTC CGGCATTATA CTGTTGGATC CTACGACAGC 
TTCGATGCGC CCAGTGACTA CATAATCAAG GAGAAGACAG TCCTCCTGCA GAAAAAAGAC 
AATGAAGGTT TTGGCTTTGT GCTGAGAGGA GCCAAGGCTC AGACCCCTAT AGAAGAGTTC 
ACTCCCACCC CAGCCTTTCC AGCACTGCAG TACCTTGAGT CTGTCGACGA GGGAGGCGTG 
GCCTGGAGAG CTGGTCTGCG AATGGGAGAC TTCCTTATAG AGGTGAATGG TCAGAATGTG 
GTTAAGGTGG GACACAGGCA GGTGGTCAAT ATGATCAGGC AAGGCGGTAA CAGTCTCATG 
GTCAAGGTTG TCATGGTTAC TCGAAATCCT GATATGGAGG AAGGGGTCAG AAAGAAAATC 
CCACAGCAGA GTAAAAGACT GAGCACACCG GCTATCGCTC TGCGCTCCAA ATCCATGACA 
TCAGAGCTGG AAGAGATGGT GGACAGAGCC TCTGCCCCAT GGAAAAAAAA ATCAGAGTTC 
GAATCTTCAC AAGCTACAGA GAAGAAGAGG ACAGTTTATC AGATGGCTTT GAATAAACTG 
GATGAGATTT TAGCAGCAGC ACAGCAGACC ATTAATACAA ATGAGGCTCC AGGGCCCCGG 
GTTCAGGGGG TAAAAAGAGA ACGGGGCAGA GGCTTTTATA ATGAGCCAAA TTTCGACCAA 
ACTGGAATGG GGATGACATC ATCTGGGGCA GGGTTAGGCT ACGACCGTGG TCAATTTGTG 
TCTGGCCATG GCCCCCCGCA CGGTATGCTG CGACAGAAGT CTATCGGGGT TCCTGAGGAG 
GAGAAGCAGT TTCTTCATCC TCCAGCCATG AAGTTTGCCC GTAGTCTTTC AGTACCTGGC 
CCAGATGAAA TCCCTCCTCC ACCTACTACA GCACCCCCTG AGCCCCCTTT TTCCTCAGCT 
CCTCCGCTTG GCTTTAGGGG CAAGACATCT CAACAGCCGT CTGTTTCTAT GTCTCAGTCT 
ACTTCCTCTG CTGCACATTA TCAACTCTAC TCACAGTCAG TACATGTTAC TCACGGACCA 
AGGGACAGGT CCCCTGGACC ACCTACTGGG TCTGGAACTG GGGACCTTGC TTATTCCCAT 
GCCCAAGCCC ACCCCTCTGT ACCAAGTAGG ACTGCCTCCA GGAAGGTGAT TGGGTACACA 
GGAGATCTTG CAGGGGCTGG AGGAGTGGCT GGGGCCAAAG CTGCAGCATT GAGAAGGGGT 
TACAGCAATG CAGTCCCACC CACCAGTGTC GCTACTGTGA TTCCACAACA GCAGCAGACT 
ACTCAGAACC AACCTTCCAG AATGGACCGG AGTGGAATGG GGGTAGGGGC TGGTGGTGTA 
CCAAAAGGCG GTGCTCGTAG AGGAAAGGGG CCCTTGGTGA AACAGTCAAA GGTGGAAGAC 
CTGCGTCAAG GTCAAGACAC TCTAGAAGCA AAGGTGTCAG TGGAGAAGAG CTCTATTCCA 
ATCCCAACTA TCATTGTTAA AGCCCCTTCC ACCAGCAGTA GTGGACGCAG TAGCCAAGGC 
AGCAGTGTGG AGGCTGAACC ACCGCCCCCA GGAGAAAGTG ACAGTTCTAA ACCAGAACCT 
GTTGTTCCCC CACCTGCACC AACCGTGCCA CCACCAGCCC CTCCATCTAT ACCTGCTCCT 
CCACCCCCAT CACTGCCATC ACTTCGTACT CAAGAGAGTC TGGACTTCAC CAGCCAGTTT 
GGTGCTGCCA TTGTAGGGGC AGCTCGCAGG GACAGAGAAC GATTTCATGA AGCACGACGC 
AAGAGTGCCT CCTTTTTTCT TTCTGCTGAG GAAGAGTTGA GTGGAGCTGG AGGAGCAGGA 
GGGCGGACTC AGACTTCTCA ACAGCAGCTG AGTTCCCAGC CTTCACCACG CCTGCGACCA 
TCCAAATCTA TAGATGAAGG CATGTTCTCA GGTGATACTT TTATCCATCA CACACGCAGT 
ATGCCTCCTG CATTTGGCTT GCCTGAGTAC TCCTCACCTG CTCCTCAAGG GGATTACCAG 
CCTAAGTCTG TACCTGCTGA CTTCTATGGG GCAGGATCCA GACAACAGCA GCAGCAGCAG 
CAGCAGCAAG CCCCCACCAC AACTTTTATT CACCCACTAA CTGGAAAAGT ACTGGACCCT 
TCATCCCCTC TAGGCTTGGC TTTGGCTGCA CGGGAACGTG CCTTGAAGGA TGATGGACGT 
ATACGTAGGG AAAGGACCAC TGAACATCAT TTTACCCGTC AGTTGTCAAG CATTGGGGCC 
TTTTCCTCAT CCACTTCACA GCCAACATCA CACATTTCCT ACCCAATGGC CTCGTCTTCC 
TCTGCAACCT CCTCTGCTGC TCAATCCACA AACACCTCTT CATATCTTGT TACCTCCTCA 
TCATTAGCTG CCACCACCAC ATCCACTCGG CCTCCATCTC CTAGGATCCT GCGTGGAGCA 
GGGAGTAGCT GGAGTGAAGA AGCTGGGGTT GGAGAGCGTG CGGATAGGGA AGCAGCTGGC 
ACCTCTGCTC CCAGAGAAGG CCTAAGGGTT CGTTTCTCCG AGGATAAAAC TGTCCACACA 
CACCACTATC AATCTCAGCA ATATCAGCCC ACCTTTAAAG AGCGTGAAAG AGAACGTCAA 
AGAGAGAGGT CAAGAGAAAG AGAGAGGGAA AGAGAGAGGG AAAGAGAAAT AGAAAAAGCT 
CAAACCCAGT ATCAGCAACA GTCATCACAA CCAATGACCC AGCAGCCTCG ACGCCCCTCA 
TTCTTGAGGA TGGAGAGTGA AGCTGGAGCG TACATTCTTT CATTAACGCC TCCATCTCCT 
CCTCCAACAG CAGCCCCTAT GCGTCCTACA GCAGGAGCCA CAGGTGAAAG TGGAAGTGGT 
ACAAGCCTGA TGGTTCTGCC TCCCCCAGCT CCCTCAGTAG ATGCAGATGA TGAGTTTGTG 
TTTGCTGATC CTCTTCCTCC ACCTCTAGAG TTTGCCAATA GTTTTGATCG TGGTTTGGGT 
TACTCTGCGA TGATTTCTCA CAATGTTGCA GCCCGTCAGC AGCGTCATCC TCCACCACCC 
CCTCCCCCAC CACCCCCGCC CACTTCGAAA GATTCCTTTA TGACAGGCTT TCCAGCTCAC 
ACACCCCTAC CGTCACCTCA GGCAGGAGAT TCCACAACTT CCAGTCTTAC CTCCTATGAC 
AGTGAGGTTG CAAACCTAAC ACAATCTGCC CCATCGCCCT CCCCATCTTC TCCCAACCCA 
CTACCACCTC ACTCCCCCTC CACCCTTCAG ACATCTGGAC CACCTCCACC TCCCTCTGTA 
TCACCTCCTC CACCACCCAA CAGCTACCAT AGACCTTTTG GCTTATCTCA GTCACAACAT 
TTATACAACA GTACACACAC AACTGGACGC TCCTCCTCTC CCACCCCACC TCTCTCTATT 
CCAGCTCCAC CTGCCTACCG GGGGCCACCA ACAGCATCAT CCACCCCTGC AACTTCTGTA 
TCAATCAGAG GTGCCTCAAC TGTGACCGCA AGCTGTTCCA CAACTGCTTC TACAACTGTC 
ACCTCTCCTA GTACTACTGC TGCTGCCACA CCACACACAC GCATAGCAAC TGTCTCCACA 
GTAGCTACAG GGGGTAGTAG GCGTCCCAGT GCAGAGCATC ATCCACCACC TAGTGCAACC 
AAGAGTGGTG AGTCCCAGGA GACAGTGGTA GATTCTGGGA TTGAGGAGCT GGACAGCCGA 
AGCAGCAGTG ATCATCACTT AGACAGCATT CTTGCACTCA GTGGTAGTGG GGTCAGAGAG 
AGGTTGGAAC GAGCAGGGAT GAGTAGCATA GGTGGAATTG GAGGGAGAGA TGGTGCAACT 
GAGGTTGACA GGACAAGTGG GGCAGACTTT TTAGAGTCTT ACATGAGCTA CCTTGATGGG 
CAGACTTTTG AAATGCACAA TGCCAAAGCC GCTAGCACTG CATCCACCTA CCCAAAAACA 
CAGAGGTACA GAGAAGGCAC CCGGGCTGGA GATCTTCGAC GCCAGACCAG CACAGCACCT 
CCTGCATACC ACCGTCGTAG AGAGGAGGAA GAGGAAGAGG ATGAGGCCGA GGAGGTAGAG 
GAGGAGGATT TGAGTGCCCG TGATGGCTAT GGGATGAGGG AAGGGCCAGG GATTGCTCGC 
TCCTCTGTAA TGTACTTTGA CCGGCCCCGC ACTCCAGAGA TAAAACCACT GTGGGGAGAT 
GGGTCGACTG GAGCATCACA GACCATGGGG GAGGGAGGGG GCCAGATAGT GGGAACATAT 
GTGGAGCCAC GTAAACTACA TCCTCCCATG ACTGGTATGA AAGCAAACAT CATTAATGAA 
CTAAGCACCA AATTACAGCA GAGAAGTAAA GGAACAGAGA ACTGGGGGGC CCAGAGATCA 
TTGAGCAGAC ATAGACACAG GTTCTCAGAG GACTCAACAG GTGCACTGAG TCCTCCCTCT 
GTCCGCTCTA GCTCCCCATC TCCAGTTCAG CTCTCTCAGC CTGCCTTGTC TCCCTCTCCC 
ACTCCTTCCT CCCAGCCACT TTACTCAAAC TGGGCTCGAT CTCCTTCCCC TCAGCCCCCT 
GCTGCCTCAT CCCAGCCCCC TCCCCGATCC CATTCACCTG TTTCCCCTTC ATCATATTCA 
CCATACCCCA CCTCACCCAA ACACCGGCCT GTTTATCGCA CCAAAGCTCT GGAGTTCCAA 
TTTATTCCCA GTCGAGAGTC ACGAAAGTCA GAGTCGCATC ATGCCCAGCG CAGACGAGCA 
CCCAGCCCTT TAATTTCTCC ATCTGACCGT CCCAAGCTTG GCCCTCCCCG CCCCTCTTCC 
CTTCCTATTC TCCCCACTAC ACCCCTCTAT AGCAGCCTGT ATGACCTTCG TGGTTCTATT 
ACTCCACCCT CACCTGCCAA TCCCCTTGGG GACCCCTACT TTTCCCCCTC CCCCCCTCTA 
TTTGCCCCCT CGGGTGCTCC CCCTCCTCCA AATTCCTCTC TGGTGGTCTC ACGGTCCCTG 
TCACCCACTC ACTTCCTGTC AGGGACTTCA TCCCCACCTC TCCACCCCAT TCCTCCACCC 
ACTTGCCTGA GTTACCCCCA TCTTCCACCC CCCTCACCCA CAAAGCCCTT TGCCTCAAAA 
CCTCTCCCAT ACTGGACCAA GTATGATGTT GCAGATTGGC TGGGCTACCT GAACCTGGGT 
GAACACAGGG AGCGATTCTT AGACAATGAA ATTGATGGCA CGCACCTGCC CTCTCTTACC 
AAGGATGACT ACCTGGACCT GGGTGTGACC CGAGTTGGCC ATCGCATGAA CATAGAGAGG 
GCGCTCAGGA GAGTTATGGA CAGAACCATC AGTAAAGATC TCATCCCCTC CATCTCCATA 
TCTTTCATCT GTTCATCTTC TAAGAGTGTG GCATCTTCAT TCATCATACT GCTCACTTTA 
CCTTACTCAT ACTGGACAAA AAGATTGAGG AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297762.1
CDS	525..8000
Translation	MTMPLSPLSSDEEQQRMLGNSQHLYPGAEEEEEEEEEMDDDGMDEEGEEENGELEMDDEEEVDDEDDIRRGPGHLRGGREEGHRQKGTMAGRLPGDRPIRPGNPGHAANPYSSNPGPTHQTSTNQQQQQPQRKLRERSSSNAPDDTHVALMVFRIGIPDIKQTKCLRFNPDATVWKAKQQVMCSLTESLRDVLNYGLFQPATDGHDAKFLEEERPLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKASLKKFMDYIQTSAVEKVAKLIDKGLDPNYHDPDTGETPLTLAVQTEPGGSEIIRVLVMGGAHIDFRARDGLTPLHKAVRAHAHTALLTLLSLGASSDYKDSRGLTPLYHTVLIGGDTSCCETLLYHRARLGLRDENGWDETHQACQNGNSQHLEHLLFYGADSSSQNASGNTALHICALYNKESCARILLYRGAKKDTKNNSGQNSFQVAVMSGHFELGEIIKNHRESDVVPFLESPKYAPQRRESSRTLGLPHPHPFLRANSDNSMNVPDWIAFPNAAGSSIVSVQGLKHGGTLRSSSSPRGARTRSPSRGRTGDRDDRSRQTRGRQGAGSISSQGTASGQRRRLYSAVPGRVFVATRSHSAQGDREISFNKGDKVKGVDKRCLLLSVGEGGYWEGTVRGRTGWFPSDCVEEVALRSLESRSESRSERAKRLFRHYTVGSYDSFDAPSDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMVDRASAPWKKKSEFESSQATEKKRTVYQMALNKLDEILAAAQQTINTNEAPGPRVQGVKRERGRGFYNEPNFDQTGMGMTSSGAGLGYDRGQFVSGHGPPHGMLRQKSIGVPEEEKQFLHPPAMKFARSLSVPGPDEIPPPPTTAPPEPPFSSAPPLGFRGKTSQQPSVSMSQSTSSAAHYQLYSQSVHVTHGPRDRSPGPPTGSGTGDLAYSHAQAHPSVPSRTASRKVIGYTGDLAGAGGVAGAKAAALRRGYSNAVPPTSVATVIPQQQQTTQNQPSRMDRSGMGVGAGGVPKGGARRGKGPLVKQSKVEDLRQGQDTLEAKVSVEKSSIPIPTIIVKAPSTSSSGRSSQGSSVEAEPPPPGESDSSKPEPVVPPPAPTVPPPAPPSIPAPPPPSLPSLRTQESLDFTSQFGAAIVGAARRDRERFHEARRKSASFFLSAEEELSGAGGAGGRTQTSQQQLSSQPSPRLRPSKSIDEGMFSGDTFIHHTRSMPPAFGLPEYSSPAPQGDYQPKSVPADFYGAGSRQQQQQQQQQAPTTTFIHPLTGKVLDPSSPLGLALAARERALKDDGRIRRERTTEHHFTRQLSSIGAFSSSTSQPTSHISYPMASSSSATSSAAQSTNTSSYLVTSSSLAATTTSTRPPSPRILRGAGSSWSEEAGVGERADREAAGTSAPREGLRVRFSEDKTVHTHHYQSQQYQPTFKERERERQRERSREREREREREREIEKAQTQYQQQSSQPMTQQPRRPSFLRMESEAGAYILSLTPPSPPPTAAPMRPTAGATGESGSGTSLMVLPPPAPSVDADDEFVFADPLPPPLEFANSFDRGLGYSAMISHNVAARQQRHPPPPPPPPPPPTSKDSFMTGFPAHTPLPSPQAGDSTTSSLTSYDSEVANLTQSAPSPSPSSPNPLPPHSPSTLQTSGPPPPPSVSPPPPPNSYHRPFGLSQSQHLYNSTHTTGRSSSPTPPLSIPAPPAYRGPPTASSTPATSVSIRGASTVTASCSTTASTTVTSPSTTAAATPHTRIATVSTVATGGSRRPSAEHHPPPSATKSGESQETVVDSGIEELDSRSSSDHHLDSILALSGSGVRERLERAGMSSIGGIGGRDGATEVDRTSGADFLESYMSYLDGQTFEMHNAKAASTASTYPKTQRYREGTRAGDLRRQTSTAPPAYHRRREEEEEEDEAEEVEEEDLSARDGYGMREGPGIARSSVMYFDRPRTPEIKPLWGDGSTGASQTMGEGGGQIVGTYVEPRKLHPPMTGMKANIINELSTKLQQRSKGTENWGAQRSLSRHRHRFSEDSTGALSPPSVRSSSPSPVQLSQPALSPSPTPSSQPLYSNWARSPSPQPPAASSQPPPRSHSPVSPSSYSPYPTSPKHRPVYRTKALEFQFIPSRESRKSESHHAQRRRAPSPLISPSDRPKLGPPRPSSLPILPTTPLYSSLYDLRGSITPPSPANPLGDPYFSPSPPLFAPSGAPPPPNSSLVVSRSLSPTHFLSGTSSPPLHPIPPPTCLSYPHLPPPSPTKPFASKPLPYWTKYDVADWLGYLNLGEHRERFLDNEIDGTHLPSLTKDDYLDLGVTRVGHRMNIERALRRVMDRTISKDLIPSISISFICSSSKSVASSFIILLTLPYSYWTKRLRN

Target ORF information:

RefSeq Version	XM_009299487.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299487.2

ORF Insert Sequence:

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGGTGTGGA TAAACGTTGT 
CTGTTGCTGA GTGTGGGTGA AGGAGGCTAT TGGGAAGGGA CGGTTCGAGG ACGCACAGGC 
TGGTTCCCCT CAGACTGCGT GGAGGAAGTT GCTCTCCGTA GCCTGGAATC TCGTTCAGAG 
AGCCGCAGTG AGAGAGCCAA GAGACTCTTC CGGCATTATA CTGTTGGATC CTACGACAGC 
TTCGATGCGC CCAGTGACTA CATAATCAAG GAGAAGACAG TCCTCCTGCA GAAAAAAGAC 
AATGAAGGTT TTGGCTTTGT GCTGAGAGGA GCCAAGGCTC AGACCCCTAT AGAAGAGTTC 
ACTCCCACCC CAGCCTTTCC AGCACTGCAG TACCTTGAGT CTGTCGACGA GGGAGGCGTG 
GCCTGGAGAG CTGGTCTGCG AATGGGAGAC TTCCTTATAG AGGTGAATGG TCAGAATGTG 
GTTAAGGTGG GACACAGGCA GGTGGTCAAT ATGATCAGGC AAGGCGGTAA CAGTCTCATG 
GTCAAGGTTG TCATGGTTAC TCGAAATCCT GATATGGAGG AAGGGGTCAG AAAGAAAATC 
CCACAGCAGA GTAAAAGACT GAGCACACCG GCTATCGCTC TGCGCTCCAA ATCCATGACA 
TCAGAGCTGG AAGAGATGGT GGACAGAGCC TCTGCCCCAT GGAAAAAAAA ATCAGAGTTC 
GAATCTTCAC AAGCTACAGA GAAGAAGAGG ACAGTTTATC AGATGGCTTT GAATAAACTG 
GATGAGATTT TAGCAGCAGC ACAGCAGACC ATTAATACAA ATGAGGCTCC AGGGCCCCGG 
GTTCAGGGGG TAAAAAGAGA ACGGGGCAGA GGCTTTTATA ATGAGCCAAA TTTCGACCAA 
ACTGGAATGG GGATGACATC ATCTGGGGCA GGGTTAGGCT ACGACCGTGG TCAATTTGTG 
TCTGGCCATG GCCCCCCGCA CGGTATGCTG CGACAGAAGT CTATCGGGGT TCCTGAGGAG 
GAGAAGCAGT TTCTTCATCC TCCAGCCATG AAGTTTGCCC GTAGTCTTTC AGTACCTGGC 
CCAGATGAAA TCCCTCCTCC ACCTACTACA GCACCCCCTG AGCCCCCTTT TTCCTCAGCT 
CCTCCGCTTG GCTTTAGGGG CAAGACATCT CAACAGCCGT CTGTTTCTAT GTCTCAGTCT 
ACTTCCTCTG CTGCACATTA TCAACTCTAC TCACAGTCAG TACATGTTAC TCACGGACCA 
AGGGACAGGT CCCCTGGACC ACCTACTGGG TCTGGAACTG GGGACCTTGC TTATTCCCAT 
GCCCAAGCCC ACCCCTCTGT ACCAAGTAGG ACTGCCTCCA GGAAGGTGAT TGGGTACACA 
GGAGATCTTG CAGGGGCTGG AGGAGTGGCT GGGGCCAAAG CTGCAGCATT GAGAAGGGGT 
TACAGCAATG CAGTCCCACC CACCAGTGTC GCTACTGTGA TTCCACAACA GCAGCAGACT 
ACTCAGAACC AACCTTCCAG AATGGACCGG AGTGGAATGG GGGTAGGGGC TGGTGGTGTA 
CCAAAAGGCG GTGCTCGTAG AGGAAAGGGG CCCTTGGTGA AACAGTCAAA GGTGGAAGAC 
CTGCGTCAAG GTCAAGACAC TCTAGAAGCA AAGGTGTCAG TGGAGAAGAG CTCTATTCCA 
ATCCCAACTA TCATTGTTAA AGCCCCTTCC ACCAGCAGTA GTGGACGCAG TAGCCAAGGC 
AGCAGTGTGG AGGCTGAACC ACCGCCCCCA GGAGAAAGTG ACAGTTCTAA ACCAGAACCT 
GTTGTTCCCC CACCTGCACC AACCGTGCCA CCACCAGCCC CTCCATCTAT ACCTGCTCCT 
CCACCCCCAT CACTGCCATC ACTTCGTACT CAAGAGAGTC TGGACTTCAC CAGCCAGTTT 
GGTGCTGCCA TTGTAGGGGC AGCTCGCAGG GACAGAGAAC GATTTCATGA AGCACGACGC 
AAGAGTGCCT CCTTTTTTCT TTCTGCTGAG GAAGAGTTGA GTGGAGCTGG AGGAGCAGGA 
GGGCGGACTC AGACTTCTCA ACAGCAGCTG AGTTCCCAGC CTTCACCACG CCTGCGACCA 
TCCAAATCTA TAGATGAAGG CATGTTCTCA GGTGATACTT TTATCCATCA CACACGCAGT 
ATGCCTCCTG CATTTGGCTT GCCTGAGTAC TCCTCACCTG CTCCTCAAGG GGATTACCAG 
CCTAAGTCTG TACCTGCTGA CTTCTATGGG GCAGGATCCA GACAACAGCA GCAGCAGCAG 
CAGCAGCAAG CCCCCACCAC AACTTTTATT CACCCACTAA CTGGAAAAGT ACTGGACCCT 
TCATCCCCTC TAGGCTTGGC TTTGGCTGCA CGGGAACGTG CCTTGAAGGA TGATGGACGT 
ATACGTAGGG AAAGGACCAC TGAACATCAT TTTACCCGTC AGTTGTCAAG CATTGGGGCC 
TTTTCCTCAT CCACTTCACA GCCAACATCA CACATTTCCT ACCCAATGGC CTCGTCTTCC 
TCTGCAACCT CCTCTGCTGC TCAATCCACA AACACCTCTT CATATCTTGT TACCTCCTCA 
TCATTAGCTG CCACCACCAC ATCCACTCGG CCTCCATCTC CTAGGATCCT GCGTGGAGCA 
GGGAGTAGCT GGAGTGAAGA AGCTGGGGTT GGAGAGCGTG CGGATAGGGA AGCAGCTGGC 
ACCTCTGCTC CCAGAGAAGG CCTAAGGGTT CGTTTCTCCG AGGATAAAAC TGTCCACACA 
CACCACTATC AATCTCAGCA ATATCAGCCC ACCTTTAAAG AGCGTGAAAG AGAACGTCAA 
AGAGAGAGGT CAAGAGAAAG AGAGAGGGAA AGAGAGAGGG AAAGAGAAAT AGAAAAAGCT 
CAAACCCAGT ATCAGCAACA GTCATCACAA CCAATGACCC AGCAGCCTCG ACGCCCCTCA 
TTCTTGAGGA TGGAGAGTGA AGCTGGAGCG TACATTCTTT CATTAACGCC TCCATCTCCT 
CCTCCAACAG CAGCCCCTAT GCGTCCTACA GCAGGAGCCA CAGGTGAAAG TGGAAGTGGT 
ACAAGCCTGA TGGTTCTGCC TCCCCCAGCT CCCTCAGTAG ATGCAGATGA TGAGTTTGTG 
TTTGCTGATC CTCTTCCTCC ACCTCTAGAG TTTGCCAATA GTTTTGATCG TGGTTTGGGT 
TACTCTGCGA TGATTTCTCA CAATGTTGCA GCCCGTCAGC AGCGTCATCC TCCACCACCC 
CCTCCCCCAC CACCCCCGCC CACTTCGAAA GATTCCTTTA TGACAGGCTT TCCAGCTCAC 
ACACCCCTAC CGTCACCTCA GGCAGGAGAT TCCACAACTT CCAGTCTTAC CTCCTATGAC 
AGTGAGGTTG CAAACCTAAC ACAATCTGCC CCATCGCCCT CCCCATCTTC TCCCAACCCA 
CTACCACCTC ACTCCCCCTC CACCCTTCAG ACATCTGGAC CACCTCCACC TCCCTCTGTA 
TCACCTCCTC CACCACCCAA CAGCTACCAT AGACCTTTTG GCTTATCTCA GTCACAACAT 
TTATACAACA GTACACACAC AACTGGACGC TCCTCCTCTC CCACCCCACC TCTCTCTATT 
CCAGCTCCAC CTGCCTACCG GGGGCCACCA ACAGCATCAT CCACCCCTGC AACTTCTGTA 
TCAATCAGAG GTGCCTCAAC TGTGACCGCA AGCTGTTCCA CAACTGCTTC TACAACTGTC 
ACCTCTCCTA GTACTACTGC TGCTGCCACA CCACACACAC GCATAGCAAC TGTCTCCACA 
GTAGCTACAG GGGGTAGTAG GCGTCCCAGT GCAGAGCATC ATCCACCACC TAGTGCAACC 
AAGAGTGGTG AGTCCCAGGA GACAGTGGTA GATTCTGGGA TTGAGGAGCT GGACAGCCGA 
AGCAGCAGTG ATCATCACTT AGACAGCATT CTTGCACTCA GTGGTAGTGG GGTCAGAGAG 
AGGTTGGAAC GAGCAGGGAT GAGTAGCATA GGTGGAATTG GAGGGAGAGA TGGTGCAACT 
GAGGTTGACA GGACAAGTGG GGCAGACTTT TTAGAGTCTT ACATGAGCTA CCTTGATGGG 
CAGACTTTTG AAATGCACAA TGCCAAAGCC GCTAGCACTG CATCCACCTA CCCAAAAACA 
CAGAGGTACA GAGAAGGCAC CCGGGCTGGA GATCTTCGAC GCCAGACCAG CACAGCACCT 
CCTGCATACC ACCGTCGTAG AGAGGAGGAA GAGGAAGAGG ATGAGGCCGA GGAGGTAGAG 
GAGGAGGATT TGAGTGCCCG TGATGGCTAT GGGATGAGGG AAGGGCCAGG GATTGCTCGC 
TCCTCTGTAA TGTACTTTGA CCGGCCCCGC ACTCCAGAGA TAAAACCACT GTGGGGAGAT 
GGGTCGACTG GAGCATCACA GACCATGGGG GAGGGAGGGG GCCAGATAGT GGGAACATAT 
GTGGAGCCAC GTAAACTACA TCCTCCCATG ACTGGTATGA AAGCAAACAT CATTAATGAA 
CTAAGCACCA AATTACAGCA GAGAAGTAAA GGAACAGAGA ACTGGGGGGC CCAGAGATCA 
TTGAGCAGAC ATAGACACAG GTTCTCAGAG GACTCAACAG GTGCACTGAG TCCTCCCTCT 
GTCCGCTCTA GCTCCCCATC TCCAGTTCAG CTCTCTCAGC CTGCCTTGTC TCCCTCTCCC 
ACTCCTTCCT CCCAGCCACT TTACTCAAAC TGGGCTCGAT CTCCTTCCCC TCAGCCCCCT 
GCTGCCTCAT CCCAGCCCCC TCCCCGATCC CATTCACCTG TTTCCCCTTC ATCATATTCA 
CCATACCCCA CCTCACCCAA ACACCGGCCT GTTTATCGCA CCAAAGCTCT GGAGTTCCAA 
TTTATTCCCA GTCGAGAGTC ACGAAAGTCA GAGTCGCATC ATGCCCAGCG CAGACGAGCA 
CCCAGCCCTT TAATTTCTCC ATCTGACCGT CCCAAGCTTG GCCCTCCCCG CCCCTCTTCC 
CTTCCTATTC TCCCCACTAC ACCCCTCTAT AGCAGCCTGT ATGACCTTCG TGGTTCTATT 
ACTCCACCCT CACCTGCCAA TCCCCTTGGG GACCCCTACT TTTCCCCCTC CCCCCCTCTA 
TTTGCCCCCT CGGGTGCTCC CCCTCCTCCA AATTCCTCTC TGGTGGTCTC ACGGTCCCTG 
TCACCCACTC ACTTCCTGTC AGGGACTTCA TCCCCACCTC TCCACCCCAT TCCTCCACCC 
ACTTGCCTGA GTTACCCCCA TCTTCCACCC CCCTCACCCA CAAAGCCCTT TGCCTCAAAA 
CCTCTCCCAT ACTGGACCAA GTATGATGTT GCAGATTGGC TGGGCTACCT GAACCTGGGT 
GAACACAGGG AGCGATTCTT AGACAATGAA ATTGATGGCA CGCACCTGCC CTCTCTTACC 
AAGGATGACT ACCTGGACCT GGGTGTGACC CGAGTTGGCC ATCGCATGAA CATAGAGAGG 
GCGCTCAGGA GAGTTATGGA CAGAACCATC AGTAAAGATC TCATCCCCTC CATCTCCATA 
TCTTTCATCT GTTCATCTTC TAAGAGTGTG GCATCTTCAT TCATCATACT GCTCACTTTA 
CCTTACTCAT ACTGGACAAA AAGATTGAGG AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_009299484.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297759.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X1, mRNA.

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CloneID	ODa45591
Clone ID Related Accession (Same CDS sequence)	XM_009299485.2 , XM_009299487.2 , XM_009299484.2
Accession Version	XM_009299484.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299484.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGGTGTGGA TAAACGTTGT 
CTGTTGCTGA GTGTGGGTGA AGGAGGCTAT TGGGAAGGGA CGGTTCGAGG ACGCACAGGC 
TGGTTCCCCT CAGACTGCGT GGAGGAAGTT GCTCTCCGTA GCCTGGAATC TCGTTCAGAG 
AGCCGCAGTG AGAGAGCCAA GAGACTCTTC CGGCATTATA CTGTTGGATC CTACGACAGC 
TTCGATGCGC CCAGTGACTA CATAATCAAG GAGAAGACAG TCCTCCTGCA GAAAAAAGAC 
AATGAAGGTT TTGGCTTTGT GCTGAGAGGA GCCAAGGCTC AGACCCCTAT AGAAGAGTTC 
ACTCCCACCC CAGCCTTTCC AGCACTGCAG TACCTTGAGT CTGTCGACGA GGGAGGCGTG 
GCCTGGAGAG CTGGTCTGCG AATGGGAGAC TTCCTTATAG AGGTGAATGG TCAGAATGTG 
GTTAAGGTGG GACACAGGCA GGTGGTCAAT ATGATCAGGC AAGGCGGTAA CAGTCTCATG 
GTCAAGGTTG TCATGGTTAC TCGAAATCCT GATATGGAGG AAGGGGTCAG AAAGAAAATC 
CCACAGCAGA GTAAAAGACT GAGCACACCG GCTATCGCTC TGCGCTCCAA ATCCATGACA 
TCAGAGCTGG AAGAGATGGT GGACAGAGCC TCTGCCCCAT GGAAAAAAAA ATCAGAGTTC 
GAATCTTCAC AAGCTACAGA GAAGAAGAGG ACAGTTTATC AGATGGCTTT GAATAAACTG 
GATGAGATTT TAGCAGCAGC ACAGCAGACC ATTAATACAA ATGAGGCTCC AGGGCCCCGG 
GTTCAGGGGG TAAAAAGAGA ACGGGGCAGA GGCTTTTATA ATGAGCCAAA TTTCGACCAA 
ACTGGAATGG GGATGACATC ATCTGGGGCA GGGTTAGGCT ACGACCGTGG TCAATTTGTG 
TCTGGCCATG GCCCCCCGCA CGGTATGCTG CGACAGAAGT CTATCGGGGT TCCTGAGGAG 
GAGAAGCAGT TTCTTCATCC TCCAGCCATG AAGTTTGCCC GTAGTCTTTC AGTACCTGGC 
CCAGATGAAA TCCCTCCTCC ACCTACTACA GCACCCCCTG AGCCCCCTTT TTCCTCAGCT 
CCTCCGCTTG GCTTTAGGGG CAAGACATCT CAACAGCCGT CTGTTTCTAT GTCTCAGTCT 
ACTTCCTCTG CTGCACATTA TCAACTCTAC TCACAGTCAG TACATGTTAC TCACGGACCA 
AGGGACAGGT CCCCTGGACC ACCTACTGGG TCTGGAACTG GGGACCTTGC TTATTCCCAT 
GCCCAAGCCC ACCCCTCTGT ACCAAGTAGG ACTGCCTCCA GGAAGGTGAT TGGGTACACA 
GGAGATCTTG CAGGGGCTGG AGGAGTGGCT GGGGCCAAAG CTGCAGCATT GAGAAGGGGT 
TACAGCAATG CAGTCCCACC CACCAGTGTC GCTACTGTGA TTCCACAACA GCAGCAGACT 
ACTCAGAACC AACCTTCCAG AATGGACCGG AGTGGAATGG GGGTAGGGGC TGGTGGTGTA 
CCAAAAGGCG GTGCTCGTAG AGGAAAGGGG CCCTTGGTGA AACAGTCAAA GGTGGAAGAC 
CTGCGTCAAG GTCAAGACAC TCTAGAAGCA AAGGTGTCAG TGGAGAAGAG CTCTATTCCA 
ATCCCAACTA TCATTGTTAA AGCCCCTTCC ACCAGCAGTA GTGGACGCAG TAGCCAAGGC 
AGCAGTGTGG AGGCTGAACC ACCGCCCCCA GGAGAAAGTG ACAGTTCTAA ACCAGAACCT 
GTTGTTCCCC CACCTGCACC AACCGTGCCA CCACCAGCCC CTCCATCTAT ACCTGCTCCT 
CCACCCCCAT CACTGCCATC ACTTCGTACT CAAGAGAGTC TGGACTTCAC CAGCCAGTTT 
GGTGCTGCCA TTGTAGGGGC AGCTCGCAGG GACAGAGAAC GATTTCATGA AGCACGACGC 
AAGAGTGCCT CCTTTTTTCT TTCTGCTGAG GAAGAGTTGA GTGGAGCTGG AGGAGCAGGA 
GGGCGGACTC AGACTTCTCA ACAGCAGCTG AGTTCCCAGC CTTCACCACG CCTGCGACCA 
TCCAAATCTA TAGATGAAGG CATGTTCTCA GGTGATACTT TTATCCATCA CACACGCAGT 
ATGCCTCCTG CATTTGGCTT GCCTGAGTAC TCCTCACCTG CTCCTCAAGG GGATTACCAG 
CCTAAGTCTG TACCTGCTGA CTTCTATGGG GCAGGATCCA GACAACAGCA GCAGCAGCAG 
CAGCAGCAAG CCCCCACCAC AACTTTTATT CACCCACTAA CTGGAAAAGT ACTGGACCCT 
TCATCCCCTC TAGGCTTGGC TTTGGCTGCA CGGGAACGTG CCTTGAAGGA TGATGGACGT 
ATACGTAGGG AAAGGACCAC TGAACATCAT TTTACCCGTC AGTTGTCAAG CATTGGGGCC 
TTTTCCTCAT CCACTTCACA GCCAACATCA CACATTTCCT ACCCAATGGC CTCGTCTTCC 
TCTGCAACCT CCTCTGCTGC TCAATCCACA AACACCTCTT CATATCTTGT TACCTCCTCA 
TCATTAGCTG CCACCACCAC ATCCACTCGG CCTCCATCTC CTAGGATCCT GCGTGGAGCA 
GGGAGTAGCT GGAGTGAAGA AGCTGGGGTT GGAGAGCGTG CGGATAGGGA AGCAGCTGGC 
ACCTCTGCTC CCAGAGAAGG CCTAAGGGTT CGTTTCTCCG AGGATAAAAC TGTCCACACA 
CACCACTATC AATCTCAGCA ATATCAGCCC ACCTTTAAAG AGCGTGAAAG AGAACGTCAA 
AGAGAGAGGT CAAGAGAAAG AGAGAGGGAA AGAGAGAGGG AAAGAGAAAT AGAAAAAGCT 
CAAACCCAGT ATCAGCAACA GTCATCACAA CCAATGACCC AGCAGCCTCG ACGCCCCTCA 
TTCTTGAGGA TGGAGAGTGA AGCTGGAGCG TACATTCTTT CATTAACGCC TCCATCTCCT 
CCTCCAACAG CAGCCCCTAT GCGTCCTACA GCAGGAGCCA CAGGTGAAAG TGGAAGTGGT 
ACAAGCCTGA TGGTTCTGCC TCCCCCAGCT CCCTCAGTAG ATGCAGATGA TGAGTTTGTG 
TTTGCTGATC CTCTTCCTCC ACCTCTAGAG TTTGCCAATA GTTTTGATCG TGGTTTGGGT 
TACTCTGCGA TGATTTCTCA CAATGTTGCA GCCCGTCAGC AGCGTCATCC TCCACCACCC 
CCTCCCCCAC CACCCCCGCC CACTTCGAAA GATTCCTTTA TGACAGGCTT TCCAGCTCAC 
ACACCCCTAC CGTCACCTCA GGCAGGAGAT TCCACAACTT CCAGTCTTAC CTCCTATGAC 
AGTGAGGTTG CAAACCTAAC ACAATCTGCC CCATCGCCCT CCCCATCTTC TCCCAACCCA 
CTACCACCTC ACTCCCCCTC CACCCTTCAG ACATCTGGAC CACCTCCACC TCCCTCTGTA 
TCACCTCCTC CACCACCCAA CAGCTACCAT AGACCTTTTG GCTTATCTCA GTCACAACAT 
TTATACAACA GTACACACAC AACTGGACGC TCCTCCTCTC CCACCCCACC TCTCTCTATT 
CCAGCTCCAC CTGCCTACCG GGGGCCACCA ACAGCATCAT CCACCCCTGC AACTTCTGTA 
TCAATCAGAG GTGCCTCAAC TGTGACCGCA AGCTGTTCCA CAACTGCTTC TACAACTGTC 
ACCTCTCCTA GTACTACTGC TGCTGCCACA CCACACACAC GCATAGCAAC TGTCTCCACA 
GTAGCTACAG GGGGTAGTAG GCGTCCCAGT GCAGAGCATC ATCCACCACC TAGTGCAACC 
AAGAGTGGTG AGTCCCAGGA GACAGTGGTA GATTCTGGGA TTGAGGAGCT GGACAGCCGA 
AGCAGCAGTG ATCATCACTT AGACAGCATT CTTGCACTCA GTGGTAGTGG GGTCAGAGAG 
AGGTTGGAAC GAGCAGGGAT GAGTAGCATA GGTGGAATTG GAGGGAGAGA TGGTGCAACT 
GAGGTTGACA GGACAAGTGG GGCAGACTTT TTAGAGTCTT ACATGAGCTA CCTTGATGGG 
CAGACTTTTG AAATGCACAA TGCCAAAGCC GCTAGCACTG CATCCACCTA CCCAAAAACA 
CAGAGGTACA GAGAAGGCAC CCGGGCTGGA GATCTTCGAC GCCAGACCAG CACAGCACCT 
CCTGCATACC ACCGTCGTAG AGAGGAGGAA GAGGAAGAGG ATGAGGCCGA GGAGGTAGAG 
GAGGAGGATT TGAGTGCCCG TGATGGCTAT GGGATGAGGG AAGGGCCAGG GATTGCTCGC 
TCCTCTGTAA TGTACTTTGA CCGGCCCCGC ACTCCAGAGA TAAAACCACT GTGGGGAGAT 
GGGTCGACTG GAGCATCACA GACCATGGGG GAGGGAGGGG GCCAGATAGT GGGAACATAT 
GTGGAGCCAC GTAAACTACA TCCTCCCATG ACTGGTATGA AAGCAAACAT CATTAATGAA 
CTAAGCACCA AATTACAGCA GAGAAGTAAA GGAACAGAGA ACTGGGGGGC CCAGAGATCA 
TTGAGCAGAC ATAGACACAG GTTCTCAGAG GACTCAACAG GTGCACTGAG TCCTCCCTCT 
GTCCGCTCTA GCTCCCCATC TCCAGTTCAG CTCTCTCAGC CTGCCTTGTC TCCCTCTCCC 
ACTCCTTCCT CCCAGCCACT TTACTCAAAC TGGGCTCGAT CTCCTTCCCC TCAGCCCCCT 
GCTGCCTCAT CCCAGCCCCC TCCCCGATCC CATTCACCTG TTTCCCCTTC ATCATATTCA 
CCATACCCCA CCTCACCCAA ACACCGGCCT GTTTATCGCA CCAAAGCTCT GGAGTTCCAA 
TTTATTCCCA GTCGAGAGTC ACGAAAGTCA GAGTCGCATC ATGCCCAGCG CAGACGAGCA 
CCCAGCCCTT TAATTTCTCC ATCTGACCGT CCCAAGCTTG GCCCTCCCCG CCCCTCTTCC 
CTTCCTATTC TCCCCACTAC ACCCCTCTAT AGCAGCCTGT ATGACCTTCG TGGTTCTATT 
ACTCCACCCT CACCTGCCAA TCCCCTTGGG GACCCCTACT TTTCCCCCTC CCCCCCTCTA 
TTTGCCCCCT CGGGTGCTCC CCCTCCTCCA AATTCCTCTC TGGTGGTCTC ACGGTCCCTG 
TCACCCACTC ACTTCCTGTC AGGGACTTCA TCCCCACCTC TCCACCCCAT TCCTCCACCC 
ACTTGCCTGA GTTACCCCCA TCTTCCACCC CCCTCACCCA CAAAGCCCTT TGCCTCAAAA 
CCTCTCCCAT ACTGGACCAA GTATGATGTT GCAGATTGGC TGGGCTACCT GAACCTGGGT 
GAACACAGGG AGCGATTCTT AGACAATGAA ATTGATGGCA CGCACCTGCC CTCTCTTACC 
AAGGATGACT ACCTGGACCT GGGTGTGACC CGAGTTGGCC ATCGCATGAA CATAGAGAGG 
GCGCTCAGGA GAGTTATGGA CAGAACCATC AGTAAAGATC TCATCCCCTC CATCTCCATA 
TCTTTCATCT GTTCATCTTC TAAGAGTGTG GCATCTTCAT TCATCATACT GCTCACTTTA 
CCTTACTCAT ACTGGACAAA AAGATTGAGG AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297759.1
CDS	670..8145
Translation	MTMPLSPLSSDEEQQRMLGNSQHLYPGAEEEEEEEEEMDDDGMDEEGEEENGELEMDDEEEVDDEDDIRRGPGHLRGGREEGHRQKGTMAGRLPGDRPIRPGNPGHAANPYSSNPGPTHQTSTNQQQQQPQRKLRERSSSNAPDDTHVALMVFRIGIPDIKQTKCLRFNPDATVWKAKQQVMCSLTESLRDVLNYGLFQPATDGHDAKFLEEERPLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKASLKKFMDYIQTSAVEKVAKLIDKGLDPNYHDPDTGETPLTLAVQTEPGGSEIIRVLVMGGAHIDFRARDGLTPLHKAVRAHAHTALLTLLSLGASSDYKDSRGLTPLYHTVLIGGDTSCCETLLYHRARLGLRDENGWDETHQACQNGNSQHLEHLLFYGADSSSQNASGNTALHICALYNKESCARILLYRGAKKDTKNNSGQNSFQVAVMSGHFELGEIIKNHRESDVVPFLESPKYAPQRRESSRTLGLPHPHPFLRANSDNSMNVPDWIAFPNAAGSSIVSVQGLKHGGTLRSSSSPRGARTRSPSRGRTGDRDDRSRQTRGRQGAGSISSQGTASGQRRRLYSAVPGRVFVATRSHSAQGDREISFNKGDKVKGVDKRCLLLSVGEGGYWEGTVRGRTGWFPSDCVEEVALRSLESRSESRSERAKRLFRHYTVGSYDSFDAPSDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMVDRASAPWKKKSEFESSQATEKKRTVYQMALNKLDEILAAAQQTINTNEAPGPRVQGVKRERGRGFYNEPNFDQTGMGMTSSGAGLGYDRGQFVSGHGPPHGMLRQKSIGVPEEEKQFLHPPAMKFARSLSVPGPDEIPPPPTTAPPEPPFSSAPPLGFRGKTSQQPSVSMSQSTSSAAHYQLYSQSVHVTHGPRDRSPGPPTGSGTGDLAYSHAQAHPSVPSRTASRKVIGYTGDLAGAGGVAGAKAAALRRGYSNAVPPTSVATVIPQQQQTTQNQPSRMDRSGMGVGAGGVPKGGARRGKGPLVKQSKVEDLRQGQDTLEAKVSVEKSSIPIPTIIVKAPSTSSSGRSSQGSSVEAEPPPPGESDSSKPEPVVPPPAPTVPPPAPPSIPAPPPPSLPSLRTQESLDFTSQFGAAIVGAARRDRERFHEARRKSASFFLSAEEELSGAGGAGGRTQTSQQQLSSQPSPRLRPSKSIDEGMFSGDTFIHHTRSMPPAFGLPEYSSPAPQGDYQPKSVPADFYGAGSRQQQQQQQQQAPTTTFIHPLTGKVLDPSSPLGLALAARERALKDDGRIRRERTTEHHFTRQLSSIGAFSSSTSQPTSHISYPMASSSSATSSAAQSTNTSSYLVTSSSLAATTTSTRPPSPRILRGAGSSWSEEAGVGERADREAAGTSAPREGLRVRFSEDKTVHTHHYQSQQYQPTFKERERERQRERSREREREREREREIEKAQTQYQQQSSQPMTQQPRRPSFLRMESEAGAYILSLTPPSPPPTAAPMRPTAGATGESGSGTSLMVLPPPAPSVDADDEFVFADPLPPPLEFANSFDRGLGYSAMISHNVAARQQRHPPPPPPPPPPPTSKDSFMTGFPAHTPLPSPQAGDSTTSSLTSYDSEVANLTQSAPSPSPSSPNPLPPHSPSTLQTSGPPPPPSVSPPPPPNSYHRPFGLSQSQHLYNSTHTTGRSSSPTPPLSIPAPPAYRGPPTASSTPATSVSIRGASTVTASCSTTASTTVTSPSTTAAATPHTRIATVSTVATGGSRRPSAEHHPPPSATKSGESQETVVDSGIEELDSRSSSDHHLDSILALSGSGVRERLERAGMSSIGGIGGRDGATEVDRTSGADFLESYMSYLDGQTFEMHNAKAASTASTYPKTQRYREGTRAGDLRRQTSTAPPAYHRRREEEEEEDEAEEVEEEDLSARDGYGMREGPGIARSSVMYFDRPRTPEIKPLWGDGSTGASQTMGEGGGQIVGTYVEPRKLHPPMTGMKANIINELSTKLQQRSKGTENWGAQRSLSRHRHRFSEDSTGALSPPSVRSSSPSPVQLSQPALSPSPTPSSQPLYSNWARSPSPQPPAASSQPPPRSHSPVSPSSYSPYPTSPKHRPVYRTKALEFQFIPSRESRKSESHHAQRRRAPSPLISPSDRPKLGPPRPSSLPILPTTPLYSSLYDLRGSITPPSPANPLGDPYFSPSPPLFAPSGAPPPPNSSLVVSRSLSPTHFLSGTSSPPLHPIPPPTCLSYPHLPPPSPTKPFASKPLPYWTKYDVADWLGYLNLGEHRERFLDNEIDGTHLPSLTKDDYLDLGVTRVGHRMNIERALRRVMDRTISKDLIPSISISFICSSSKSVASSFIILLTLPYSYWTKRLRN

Target ORF information:

RefSeq Version	XM_009299484.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299484.2

ORF Insert Sequence:

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGGTGTGGA TAAACGTTGT 
CTGTTGCTGA GTGTGGGTGA AGGAGGCTAT TGGGAAGGGA CGGTTCGAGG ACGCACAGGC 
TGGTTCCCCT CAGACTGCGT GGAGGAAGTT GCTCTCCGTA GCCTGGAATC TCGTTCAGAG 
AGCCGCAGTG AGAGAGCCAA GAGACTCTTC CGGCATTATA CTGTTGGATC CTACGACAGC 
TTCGATGCGC CCAGTGACTA CATAATCAAG GAGAAGACAG TCCTCCTGCA GAAAAAAGAC 
AATGAAGGTT TTGGCTTTGT GCTGAGAGGA GCCAAGGCTC AGACCCCTAT AGAAGAGTTC 
ACTCCCACCC CAGCCTTTCC AGCACTGCAG TACCTTGAGT CTGTCGACGA GGGAGGCGTG 
GCCTGGAGAG CTGGTCTGCG AATGGGAGAC TTCCTTATAG AGGTGAATGG TCAGAATGTG 
GTTAAGGTGG GACACAGGCA GGTGGTCAAT ATGATCAGGC AAGGCGGTAA CAGTCTCATG 
GTCAAGGTTG TCATGGTTAC TCGAAATCCT GATATGGAGG AAGGGGTCAG AAAGAAAATC 
CCACAGCAGA GTAAAAGACT GAGCACACCG GCTATCGCTC TGCGCTCCAA ATCCATGACA 
TCAGAGCTGG AAGAGATGGT GGACAGAGCC TCTGCCCCAT GGAAAAAAAA ATCAGAGTTC 
GAATCTTCAC AAGCTACAGA GAAGAAGAGG ACAGTTTATC AGATGGCTTT GAATAAACTG 
GATGAGATTT TAGCAGCAGC ACAGCAGACC ATTAATACAA ATGAGGCTCC AGGGCCCCGG 
GTTCAGGGGG TAAAAAGAGA ACGGGGCAGA GGCTTTTATA ATGAGCCAAA TTTCGACCAA 
ACTGGAATGG GGATGACATC ATCTGGGGCA GGGTTAGGCT ACGACCGTGG TCAATTTGTG 
TCTGGCCATG GCCCCCCGCA CGGTATGCTG CGACAGAAGT CTATCGGGGT TCCTGAGGAG 
GAGAAGCAGT TTCTTCATCC TCCAGCCATG AAGTTTGCCC GTAGTCTTTC AGTACCTGGC 
CCAGATGAAA TCCCTCCTCC ACCTACTACA GCACCCCCTG AGCCCCCTTT TTCCTCAGCT 
CCTCCGCTTG GCTTTAGGGG CAAGACATCT CAACAGCCGT CTGTTTCTAT GTCTCAGTCT 
ACTTCCTCTG CTGCACATTA TCAACTCTAC TCACAGTCAG TACATGTTAC TCACGGACCA 
AGGGACAGGT CCCCTGGACC ACCTACTGGG TCTGGAACTG GGGACCTTGC TTATTCCCAT 
GCCCAAGCCC ACCCCTCTGT ACCAAGTAGG ACTGCCTCCA GGAAGGTGAT TGGGTACACA 
GGAGATCTTG CAGGGGCTGG AGGAGTGGCT GGGGCCAAAG CTGCAGCATT GAGAAGGGGT 
TACAGCAATG CAGTCCCACC CACCAGTGTC GCTACTGTGA TTCCACAACA GCAGCAGACT 
ACTCAGAACC AACCTTCCAG AATGGACCGG AGTGGAATGG GGGTAGGGGC TGGTGGTGTA 
CCAAAAGGCG GTGCTCGTAG AGGAAAGGGG CCCTTGGTGA AACAGTCAAA GGTGGAAGAC 
CTGCGTCAAG GTCAAGACAC TCTAGAAGCA AAGGTGTCAG TGGAGAAGAG CTCTATTCCA 
ATCCCAACTA TCATTGTTAA AGCCCCTTCC ACCAGCAGTA GTGGACGCAG TAGCCAAGGC 
AGCAGTGTGG AGGCTGAACC ACCGCCCCCA GGAGAAAGTG ACAGTTCTAA ACCAGAACCT 
GTTGTTCCCC CACCTGCACC AACCGTGCCA CCACCAGCCC CTCCATCTAT ACCTGCTCCT 
CCACCCCCAT CACTGCCATC ACTTCGTACT CAAGAGAGTC TGGACTTCAC CAGCCAGTTT 
GGTGCTGCCA TTGTAGGGGC AGCTCGCAGG GACAGAGAAC GATTTCATGA AGCACGACGC 
AAGAGTGCCT CCTTTTTTCT TTCTGCTGAG GAAGAGTTGA GTGGAGCTGG AGGAGCAGGA 
GGGCGGACTC AGACTTCTCA ACAGCAGCTG AGTTCCCAGC CTTCACCACG CCTGCGACCA 
TCCAAATCTA TAGATGAAGG CATGTTCTCA GGTGATACTT TTATCCATCA CACACGCAGT 
ATGCCTCCTG CATTTGGCTT GCCTGAGTAC TCCTCACCTG CTCCTCAAGG GGATTACCAG 
CCTAAGTCTG TACCTGCTGA CTTCTATGGG GCAGGATCCA GACAACAGCA GCAGCAGCAG 
CAGCAGCAAG CCCCCACCAC AACTTTTATT CACCCACTAA CTGGAAAAGT ACTGGACCCT 
TCATCCCCTC TAGGCTTGGC TTTGGCTGCA CGGGAACGTG CCTTGAAGGA TGATGGACGT 
ATACGTAGGG AAAGGACCAC TGAACATCAT TTTACCCGTC AGTTGTCAAG CATTGGGGCC 
TTTTCCTCAT CCACTTCACA GCCAACATCA CACATTTCCT ACCCAATGGC CTCGTCTTCC 
TCTGCAACCT CCTCTGCTGC TCAATCCACA AACACCTCTT CATATCTTGT TACCTCCTCA 
TCATTAGCTG CCACCACCAC ATCCACTCGG CCTCCATCTC CTAGGATCCT GCGTGGAGCA 
GGGAGTAGCT GGAGTGAAGA AGCTGGGGTT GGAGAGCGTG CGGATAGGGA AGCAGCTGGC 
ACCTCTGCTC CCAGAGAAGG CCTAAGGGTT CGTTTCTCCG AGGATAAAAC TGTCCACACA 
CACCACTATC AATCTCAGCA ATATCAGCCC ACCTTTAAAG AGCGTGAAAG AGAACGTCAA 
AGAGAGAGGT CAAGAGAAAG AGAGAGGGAA AGAGAGAGGG AAAGAGAAAT AGAAAAAGCT 
CAAACCCAGT ATCAGCAACA GTCATCACAA CCAATGACCC AGCAGCCTCG ACGCCCCTCA 
TTCTTGAGGA TGGAGAGTGA AGCTGGAGCG TACATTCTTT CATTAACGCC TCCATCTCCT 
CCTCCAACAG CAGCCCCTAT GCGTCCTACA GCAGGAGCCA CAGGTGAAAG TGGAAGTGGT 
ACAAGCCTGA TGGTTCTGCC TCCCCCAGCT CCCTCAGTAG ATGCAGATGA TGAGTTTGTG 
TTTGCTGATC CTCTTCCTCC ACCTCTAGAG TTTGCCAATA GTTTTGATCG TGGTTTGGGT 
TACTCTGCGA TGATTTCTCA CAATGTTGCA GCCCGTCAGC AGCGTCATCC TCCACCACCC 
CCTCCCCCAC CACCCCCGCC CACTTCGAAA GATTCCTTTA TGACAGGCTT TCCAGCTCAC 
ACACCCCTAC CGTCACCTCA GGCAGGAGAT TCCACAACTT CCAGTCTTAC CTCCTATGAC 
AGTGAGGTTG CAAACCTAAC ACAATCTGCC CCATCGCCCT CCCCATCTTC TCCCAACCCA 
CTACCACCTC ACTCCCCCTC CACCCTTCAG ACATCTGGAC CACCTCCACC TCCCTCTGTA 
TCACCTCCTC CACCACCCAA CAGCTACCAT AGACCTTTTG GCTTATCTCA GTCACAACAT 
TTATACAACA GTACACACAC AACTGGACGC TCCTCCTCTC CCACCCCACC TCTCTCTATT 
CCAGCTCCAC CTGCCTACCG GGGGCCACCA ACAGCATCAT CCACCCCTGC AACTTCTGTA 
TCAATCAGAG GTGCCTCAAC TGTGACCGCA AGCTGTTCCA CAACTGCTTC TACAACTGTC 
ACCTCTCCTA GTACTACTGC TGCTGCCACA CCACACACAC GCATAGCAAC TGTCTCCACA 
GTAGCTACAG GGGGTAGTAG GCGTCCCAGT GCAGAGCATC ATCCACCACC TAGTGCAACC 
AAGAGTGGTG AGTCCCAGGA GACAGTGGTA GATTCTGGGA TTGAGGAGCT GGACAGCCGA 
AGCAGCAGTG ATCATCACTT AGACAGCATT CTTGCACTCA GTGGTAGTGG GGTCAGAGAG 
AGGTTGGAAC GAGCAGGGAT GAGTAGCATA GGTGGAATTG GAGGGAGAGA TGGTGCAACT 
GAGGTTGACA GGACAAGTGG GGCAGACTTT TTAGAGTCTT ACATGAGCTA CCTTGATGGG 
CAGACTTTTG AAATGCACAA TGCCAAAGCC GCTAGCACTG CATCCACCTA CCCAAAAACA 
CAGAGGTACA GAGAAGGCAC CCGGGCTGGA GATCTTCGAC GCCAGACCAG CACAGCACCT 
CCTGCATACC ACCGTCGTAG AGAGGAGGAA GAGGAAGAGG ATGAGGCCGA GGAGGTAGAG 
GAGGAGGATT TGAGTGCCCG TGATGGCTAT GGGATGAGGG AAGGGCCAGG GATTGCTCGC 
TCCTCTGTAA TGTACTTTGA CCGGCCCCGC ACTCCAGAGA TAAAACCACT GTGGGGAGAT 
GGGTCGACTG GAGCATCACA GACCATGGGG GAGGGAGGGG GCCAGATAGT GGGAACATAT 
GTGGAGCCAC GTAAACTACA TCCTCCCATG ACTGGTATGA AAGCAAACAT CATTAATGAA 
CTAAGCACCA AATTACAGCA GAGAAGTAAA GGAACAGAGA ACTGGGGGGC CCAGAGATCA 
TTGAGCAGAC ATAGACACAG GTTCTCAGAG GACTCAACAG GTGCACTGAG TCCTCCCTCT 
GTCCGCTCTA GCTCCCCATC TCCAGTTCAG CTCTCTCAGC CTGCCTTGTC TCCCTCTCCC 
ACTCCTTCCT CCCAGCCACT TTACTCAAAC TGGGCTCGAT CTCCTTCCCC TCAGCCCCCT 
GCTGCCTCAT CCCAGCCCCC TCCCCGATCC CATTCACCTG TTTCCCCTTC ATCATATTCA 
CCATACCCCA CCTCACCCAA ACACCGGCCT GTTTATCGCA CCAAAGCTCT GGAGTTCCAA 
TTTATTCCCA GTCGAGAGTC ACGAAAGTCA GAGTCGCATC ATGCCCAGCG CAGACGAGCA 
CCCAGCCCTT TAATTTCTCC ATCTGACCGT CCCAAGCTTG GCCCTCCCCG CCCCTCTTCC 
CTTCCTATTC TCCCCACTAC ACCCCTCTAT AGCAGCCTGT ATGACCTTCG TGGTTCTATT 
ACTCCACCCT CACCTGCCAA TCCCCTTGGG GACCCCTACT TTTCCCCCTC CCCCCCTCTA 
TTTGCCCCCT CGGGTGCTCC CCCTCCTCCA AATTCCTCTC TGGTGGTCTC ACGGTCCCTG 
TCACCCACTC ACTTCCTGTC AGGGACTTCA TCCCCACCTC TCCACCCCAT TCCTCCACCC 
ACTTGCCTGA GTTACCCCCA TCTTCCACCC CCCTCACCCA CAAAGCCCTT TGCCTCAAAA 
CCTCTCCCAT ACTGGACCAA GTATGATGTT GCAGATTGGC TGGGCTACCT GAACCTGGGT 
GAACACAGGG AGCGATTCTT AGACAATGAA ATTGATGGCA CGCACCTGCC CTCTCTTACC 
AAGGATGACT ACCTGGACCT GGGTGTGACC CGAGTTGGCC ATCGCATGAA CATAGAGAGG 
GCGCTCAGGA GAGTTATGGA CAGAACCATC AGTAAAGATC TCATCCCCTC CATCTCCATA 
TCTTTCATCT GTTCATCTTC TAAGAGTGTG GCATCTTCAT TCATCATACT GCTCACTTTA 
CCTTACTCAT ACTGGACAAA AAGATTGAGG AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_009299489.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297764.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X4, mRNA.

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CloneID	ODa45592
Clone ID Related Accession (Same CDS sequence)	XM_009299489.2
Accession Version	XM_009299489.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299489.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGGTGTGGA TAAACGTTGT 
CTGTTGCTGA GTGTGGGTGA AGGAGGCTAT TGGGAAGGGA CGGTTCGAGG ACGCACAGGC 
TGGTTCCCCT CAGACTGCGT GGAGGAAGTT GCTCTCCGTA GCCTGGAATC TCGTTCAGAG 
AGCCGCAGTG AGAGAGCCAA GAGACTCTTC CGGCATTATA CTGTTGGATC CTACGACAGC 
TTCGATGCGC CCAGTGACTA CATAATCAAG GAGAAGACAG TCCTCCTGCA GAAAAAAGAC 
AATGAAGGTT TTGGCTTTGT GCTGAGAGGA GCCAAGGCTC AGACCCCTAT AGAAGAGTTC 
ACTCCCACCC CAGCCTTTCC AGCACTGCAG TACCTTGAGT CTGTCGACGA GGGAGGCGTG 
GCCTGGAGAG CTGGTCTGCG AATGGGAGAC TTCCTTATAG AGGTGAATGG TCAGAATGTG 
GTTAAGGTGG GACACAGGCA GGTGGTCAAT ATGATCAGGC AAGGCGGTAA CAGTCTCATG 
GTCAAGGTTG TCATGGTTAC TCGAAATCCT GATATGGAGG AAGGGGTCAG AAAGAAAATC 
CCACAGCAGA GTAAAAGACT GAGCACACCG GCTATCGCTC TGCGCTCCAA ATCCATGACA 
TCAGAGCTGG AAGAGATGGT GGACAGAGCC TCTGCCCCAT GGAAAAAAAA ATCAGAGTTC 
GAATCTTCAC AAGCTACAGA GAAGAAGAGG ACAGTTTATC AGATGGCTTT GAATAAACTG 
GATGAGATTT TAGCAGCAGC ACAGCAGACC ATTAATACAA ATGAGGCTCC AGGGCCCCGG 
GTTCAGGGGG TAAAAAGAGA ACGGGGCAGA GGCTTTTATA ATGAGCCAAA TTTCGACCAA 
ACTGGAATGG GGATGACATC ATCTGGGGCA GGGTTAGGCT ACGACCGTGG TCAATTTGTG 
TCTGGCCATG GCCCCCCGCA CGGTATGCTG CGACAGAAGT CTATCGGGGT TCCTGAGGAG 
GAGAAGCAGT TTCTTCATCC TCCAGCCATG AAGTTTGCCC GTAGTCTTTC AGTACCTGGC 
CCAGATGAAA TCCCTCCTCC ACCTACTACA GCACCCCCTG AGCCCCCTTT TTCCTCAGCT 
CCTCCGCTTG GCTTTAGGGG CAAGACATCT CAACAGCCGT CTGTTTCTAT GTCTCAGTCT 
ACTTCCTCTG CTGCACATTA TCAACTCTAC TCACAGTCAG TACATGTTAC TCACGGACCA 
AGGGACAGGT CCCCTGGACC ACCTACTGGG TCTGGAACTG GGGACCTTGC TTATTCCCAT 
GCCCAAGCCC ACCCCTCTGT ACCAAGTAGG ACTGCCTCCA GGAAGGTGAT TGGGTACACA 
GGAGATCTTG CAGGGGCTGG AGGAGTGGCT GGGGCCAAAG CTGCAGCATT GAGAAGGGGT 
TACAGCAATG CAGTCCCACC CACCAGTGTC GCTACTGTGA TTCCACAACA GCAGCAGACT 
ACTCAGAACC AACCTTCCAG AATGGACCGG AGTGGAATGG GGGTAGGGGC TGGTGGTGTA 
CCAAAAGGCG GTGCTCGTAG AGGAAAGGGG CCCTTGGTGA AACAGTCAAA GGTGGAAGAC 
CTGCGTCAAG GTCAAGACAC TCTAGAAGCA AAGGTGTCAG TGGAGAAGAG CTCTATTCCA 
ATCCCAACTA TCATTGTTAA AGCCCCTTCC ACCAGCAGTA GTGGACGCAG TAGCCAAGGC 
AGCAGTGTGG AGGCTGAACC ACCGCCCCCA GGAGAAAGTG ACAGTTCTAA ACCAGAACCT 
GTTGTTCCCC CACCTGCACC AACCGTGCCA CCACCAGCCC CTCCATCTAT ACCTGCTCCT 
CCACCCCCAT CACTGCCATC ACTTCGTACT CAAGAGAGTC TGGACTTCAC CAGCCAGTTT 
GGTGCTGCCA TTGTAGGGGC AGCTCGCAGG GACAGAGAAC GATTTCATGA AGCACGACGC 
AAGAGTGCCT CCTTTTTTCT TTCTGCTGAG GAAGAGTTGA GTGGAGCTGG AGGAGCAGGA 
GGGCGGACTC AGACTTCTCA ACAGCAGCTG AGTTCCCAGC CTTCACCACG CCTGCGACCA 
TCCAAATCTA TAGATGAAGG CATGTTCTCA GGTGATACTT TTATCCATCA CACACGCAGT 
ATGCCTCCTG CATTTGGCTT GCCTGAGTAC TCCTCACCTG CTCCTCAAGG GGATTACCAG 
CCTAAGTCTG TACCTGCTGA CTTCTATGGG GCAGGATCCA GACAACAGCA GCAGCAGCAG 
CAGCAGCAAG CCCCCACCAC AACTTTTATT CACCCACTAA CTGGAAAAGT ACTGGACCCT 
TCATCCCCTC TAGGCTTGGC TTTGGCTGCA CGGGAACGTG CCTTGAAGGA TGATGGACGT 
ATACGTAGGG AAAGGACCAC TGAACATCAT TTTACCCGTC AGTTGTCAAG CATTGGGGCC 
TTTTCCTCAT CCACTTCACA GCCAACATCA CACATTTCCT ACCCAATGGC CTCGTCTTCC 
TCTGCAACCT CCTCTGCTGC TCAATCCACA AACACCTCTT CATATCTTGT TACCTCCTCA 
TCATTAGCTG CCACCACCAC ATCCACTCGG CCTCCATCTC CTAGGATCCT GCGTGGAGCA 
GGGAGTAGCT GGAGTGAAGA AGCTGGGGTT GGAGAGCGTG CGGATAGGGA AGCAGCTGGC 
ACCTCTGCTC CCAGAGAAGG CCTAAGGGTT CGTTTCTCCG AGGATAAAAC TGTCCACACA 
CACCACTATC AATCTCAGCA ATATCAGCCC ACCTTTAAAG AGCGTGAAAG AGAACGTCAA 
AGAGAGAGGT CAAGAGAAAG AGAGAGGGAA AGAGAGAGGG AAAGAGAAAT AGAAAAAGCT 
CAAACCCAGT ATCAGCAACA GTCATCACAA CCAATGACCC AGCAGCCTCG ACGCCCCTCA 
TTCTTGAGGA TGGAGAGTGA AGCTGGAGCG TACATTCTTT CATTAACGCC TCCATCTCCT 
CCTCCAACAG CAGCCCCTAT GCGTCCTACA GCAGGAGCCA CAGGTGAAAG TGGAAGTGGT 
ACAAGCCTGA TGGTTCTGCC TCCCCCAGCT CCCTCAGTAG ATGCAGATGA TGAGTTTGTG 
TTTGCTGATC CTCTTCCTCC ACCTCTAGAG TTTGCCAATA GTTTTGATCG TGGTTTGGGT 
TACTCTGCGA TGATTTCTCA CAATGTTGCA GCCCGTCAGC AGCGTCATCC TCCACCACCC 
CCTCCCCCAC CACCCCCGCC CACTTCGAAA GATTCCTTTA TGACAGGCTT TCCAGCTCAC 
ACACCCCTAC CGTCACCTCA GGCAGGAGAT TCCACAACTT CCAGTCTTAC CTCCTATGAC 
AGTGAGGTTG CAAACCTAAC ACAATCTGCC CCATCGCCCT CCCCATCTTC TCCCAACCCA 
CTACCACCTC ACTCCCCCTC CACCCTTCAG ACATCTGGAC CACCTCCACC TCCCTCTGTA 
TCACCTCCTC CACCACCCAA CAGCTACCAT AGACCTTTTG GCTTATCTCA GTCACAACAT 
TTATACAACA GTACACACAC AACTGGACGC TCCTCCTCTC CCACCCCACC TCTCTCTATT 
CCAGCTCCAC CTGCCTACCG GGGGCCACCA ACAGCATCAT CCACCCCTGC AACTTCTGTA 
TCAATCAGAG GTGCCTCAAC TGTGACCGCA AGCTGTTCCA CAACTGCTTC TACAACTGTC 
ACCTCTCCTA GTACTACTGC TGCTGCCACA CCACACACAC GCATAGCAAC TGTCTCCACA 
GTAGCTACAG GGGGTAGTAG GCGTCCCAGT GCAGAGCATC ATCCACCACC TAGTGCAACC 
AAGAGTGGTG AGTCCCAGGA GACAGTGGTA GATTCTGGGA TTGAGGAGCT GGACAGCCGA 
AGCAGCAGTG ATCATCACTT AGACAGCATT CTTGCACTCA GTGGTAGTGG GGTCAGAGAG 
AGGTTGGAAC GAGCAGGGAT GAGTAGCATA GGTGGAATTG GAGGGAGAGA TGGTGCAACT 
GAGGTTGACA GGACAAGTGG GGCAGACTTT TTAGAGTCTT ACATGAGCTA CCTTGATGGG 
CAGACTTTTG AAATGCACAA TGCCAAAGCC GCTAGCACTG CATCCACCTA CCCAAAAACA 
CAGAGGTACA GAGAAGGCAC CCGGGCTGGA GATCTTCGAC GCCAGACCAG CACAGCACCT 
CCTGCATACC ACCGTCGTAG AGAGGAGGAA GAGGAAGAGG ATGAGGCCGA GGAGGTAGAG 
GAGGAGGATT TGAGTGCCCG TGATGGCTAT GGGATGAGGG AAGGGCCAGG GATTGCTCGC 
TCCTCTGTAA TGTACTTTGA CCGGCCCCGC ACTCCAGAGA TAAAACCACT GTGGGGAGAT 
GGGTCGACTG GAGCATCACA GACCATGGGG GAGGGAGGGG GCCAGATAGT GGGAACATAT 
GTGGAGCCAC GTAAACTACA TCCTCCCATG ACTGGTATGA AAGCAAACAT CATTAATGAA 
CTAAGCACCA AATTACAGCA GAGAAGTAAA GGAACAGAGA ACTGGGGGGC CCAGAGATCA 
TTGAGCAGAC ATAGGTTCTC AGAGGACTCA ACAGGTGCAC TGAGTCCTCC CTCTGTCCGC 
TCTAGCTCCC CATCTCCAGT TCAGCTCTCT CAGCCTGCCT TGTCTCCCTC TCCCACTCCT 
TCCTCCCAGC CACTTTACTC AAACTGGGCT CGATCTCCTT CCCCTCAGCC CCCTGCTGCC 
TCATCCCAGC CCCCTCCCCG ATCCCATTCA CCTGTTTCCC CTTCATCATA TTCACCATAC 
CCCACCTCAC CCAAACACCG GCCTGTTTAT CGCACCAAAG CTCTGGAGTT CCAATTTATT 
CCCAGTCGAG AGTCACGAAA GTCAGAGTCG CATCATGCCC AGCGCAGACG AGCACCCAGC 
CCTTTAATTT CTCCATCTGA CCGTCCCAAG CTTGGCCCTC CCCGCCCCTC TTCCCTTCCT 
ATTCTCCCCA CTACACCCCT CTATAGCAGC CTGTATGACC TTCGTGGTTC TATTACTCCA 
CCCTCACCTG CCAATCCCCT TGGGGACCCC TACTTTTCCC CCTCCCCCCC TCTATTTGCC 
CCCTCGGGTG CTCCCCCTCC TCCAAATTCC TCTCTGGTGG TCTCACGGTC CCTGTCACCC 
ACTCACTTCC TGTCAGGGAC TTCATCCCCA CCTCTCCACC CCATTCCTCC ACCCACTTGC 
CTGAGTTACC CCCATCTTCC ACCCCCCTCA CCCACAAAGC CCTTTGCCTC AAAACCTCTC 
CCATACTGGA CCAAGTATGA TGTTGCAGAT TGGCTGGGCT ACCTGAACCT GGGTGAACAC 
AGGGAGCGAT TCTTAGACAA TGAAATTGAT GGCACGCACC TGCCCTCTCT TACCAAGGAT 
GACTACCTGG ACCTGGGTGT GACCCGAGTT GGCCATCGCA TGAACATAGA GAGGGCGCTC 
AGGAGAGTTA TGGACAGAAC CATCAGTAAA GATCTCATCC CCTCCATCTC CATATCTTTC 
ATCTGTTCAT CTTCTAAGAG TGTGGCATCT TCATTCATCA TACTGCTCAC TTTACCTTAC 
TCATACTGGA CAAAAAGATT GAGGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297764.1
CDS	825..8294
Translation	MTMPLSPLSSDEEQQRMLGNSQHLYPGAEEEEEEEEEMDDDGMDEEGEEENGELEMDDEEEVDDEDDIRRGPGHLRGGREEGHRQKGTMAGRLPGDRPIRPGNPGHAANPYSSNPGPTHQTSTNQQQQQPQRKLRERSSSNAPDDTHVALMVFRIGIPDIKQTKCLRFNPDATVWKAKQQVMCSLTESLRDVLNYGLFQPATDGHDAKFLEEERPLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKASLKKFMDYIQTSAVEKVAKLIDKGLDPNYHDPDTGETPLTLAVQTEPGGSEIIRVLVMGGAHIDFRARDGLTPLHKAVRAHAHTALLTLLSLGASSDYKDSRGLTPLYHTVLIGGDTSCCETLLYHRARLGLRDENGWDETHQACQNGNSQHLEHLLFYGADSSSQNASGNTALHICALYNKESCARILLYRGAKKDTKNNSGQNSFQVAVMSGHFELGEIIKNHRESDVVPFLESPKYAPQRRESSRTLGLPHPHPFLRANSDNSMNVPDWIAFPNAAGSSIVSVQGLKHGGTLRSSSSPRGARTRSPSRGRTGDRDDRSRQTRGRQGAGSISSQGTASGQRRRLYSAVPGRVFVATRSHSAQGDREISFNKGDKVKGVDKRCLLLSVGEGGYWEGTVRGRTGWFPSDCVEEVALRSLESRSESRSERAKRLFRHYTVGSYDSFDAPSDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMVDRASAPWKKKSEFESSQATEKKRTVYQMALNKLDEILAAAQQTINTNEAPGPRVQGVKRERGRGFYNEPNFDQTGMGMTSSGAGLGYDRGQFVSGHGPPHGMLRQKSIGVPEEEKQFLHPPAMKFARSLSVPGPDEIPPPPTTAPPEPPFSSAPPLGFRGKTSQQPSVSMSQSTSSAAHYQLYSQSVHVTHGPRDRSPGPPTGSGTGDLAYSHAQAHPSVPSRTASRKVIGYTGDLAGAGGVAGAKAAALRRGYSNAVPPTSVATVIPQQQQTTQNQPSRMDRSGMGVGAGGVPKGGARRGKGPLVKQSKVEDLRQGQDTLEAKVSVEKSSIPIPTIIVKAPSTSSSGRSSQGSSVEAEPPPPGESDSSKPEPVVPPPAPTVPPPAPPSIPAPPPPSLPSLRTQESLDFTSQFGAAIVGAARRDRERFHEARRKSASFFLSAEEELSGAGGAGGRTQTSQQQLSSQPSPRLRPSKSIDEGMFSGDTFIHHTRSMPPAFGLPEYSSPAPQGDYQPKSVPADFYGAGSRQQQQQQQQQAPTTTFIHPLTGKVLDPSSPLGLALAARERALKDDGRIRRERTTEHHFTRQLSSIGAFSSSTSQPTSHISYPMASSSSATSSAAQSTNTSSYLVTSSSLAATTTSTRPPSPRILRGAGSSWSEEAGVGERADREAAGTSAPREGLRVRFSEDKTVHTHHYQSQQYQPTFKERERERQRERSREREREREREREIEKAQTQYQQQSSQPMTQQPRRPSFLRMESEAGAYILSLTPPSPPPTAAPMRPTAGATGESGSGTSLMVLPPPAPSVDADDEFVFADPLPPPLEFANSFDRGLGYSAMISHNVAARQQRHPPPPPPPPPPPTSKDSFMTGFPAHTPLPSPQAGDSTTSSLTSYDSEVANLTQSAPSPSPSSPNPLPPHSPSTLQTSGPPPPPSVSPPPPPNSYHRPFGLSQSQHLYNSTHTTGRSSSPTPPLSIPAPPAYRGPPTASSTPATSVSIRGASTVTASCSTTASTTVTSPSTTAAATPHTRIATVSTVATGGSRRPSAEHHPPPSATKSGESQETVVDSGIEELDSRSSSDHHLDSILALSGSGVRERLERAGMSSIGGIGGRDGATEVDRTSGADFLESYMSYLDGQTFEMHNAKAASTASTYPKTQRYREGTRAGDLRRQTSTAPPAYHRRREEEEEEDEAEEVEEEDLSARDGYGMREGPGIARSSVMYFDRPRTPEIKPLWGDGSTGASQTMGEGGGQIVGTYVEPRKLHPPMTGMKANIINELSTKLQQRSKGTENWGAQRSLSRHRFSEDSTGALSPPSVRSSSPSPVQLSQPALSPSPTPSSQPLYSNWARSPSPQPPAASSQPPPRSHSPVSPSSYSPYPTSPKHRPVYRTKALEFQFIPSRESRKSESHHAQRRRAPSPLISPSDRPKLGPPRPSSLPILPTTPLYSSLYDLRGSITPPSPANPLGDPYFSPSPPLFAPSGAPPPPNSSLVVSRSLSPTHFLSGTSSPPLHPIPPPTCLSYPHLPPPSPTKPFASKPLPYWTKYDVADWLGYLNLGEHRERFLDNEIDGTHLPSLTKDDYLDLGVTRVGHRMNIERALRRVMDRTISKDLIPSISISFICSSSKSVASSFIILLTLPYSYWTKRLRN

Target ORF information:

RefSeq Version	XM_009299489.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299489.2

ORF Insert Sequence:

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGGTGTGGA TAAACGTTGT 
CTGTTGCTGA GTGTGGGTGA AGGAGGCTAT TGGGAAGGGA CGGTTCGAGG ACGCACAGGC 
TGGTTCCCCT CAGACTGCGT GGAGGAAGTT GCTCTCCGTA GCCTGGAATC TCGTTCAGAG 
AGCCGCAGTG AGAGAGCCAA GAGACTCTTC CGGCATTATA CTGTTGGATC CTACGACAGC 
TTCGATGCGC CCAGTGACTA CATAATCAAG GAGAAGACAG TCCTCCTGCA GAAAAAAGAC 
AATGAAGGTT TTGGCTTTGT GCTGAGAGGA GCCAAGGCTC AGACCCCTAT AGAAGAGTTC 
ACTCCCACCC CAGCCTTTCC AGCACTGCAG TACCTTGAGT CTGTCGACGA GGGAGGCGTG 
GCCTGGAGAG CTGGTCTGCG AATGGGAGAC TTCCTTATAG AGGTGAATGG TCAGAATGTG 
GTTAAGGTGG GACACAGGCA GGTGGTCAAT ATGATCAGGC AAGGCGGTAA CAGTCTCATG 
GTCAAGGTTG TCATGGTTAC TCGAAATCCT GATATGGAGG AAGGGGTCAG AAAGAAAATC 
CCACAGCAGA GTAAAAGACT GAGCACACCG GCTATCGCTC TGCGCTCCAA ATCCATGACA 
TCAGAGCTGG AAGAGATGGT GGACAGAGCC TCTGCCCCAT GGAAAAAAAA ATCAGAGTTC 
GAATCTTCAC AAGCTACAGA GAAGAAGAGG ACAGTTTATC AGATGGCTTT GAATAAACTG 
GATGAGATTT TAGCAGCAGC ACAGCAGACC ATTAATACAA ATGAGGCTCC AGGGCCCCGG 
GTTCAGGGGG TAAAAAGAGA ACGGGGCAGA GGCTTTTATA ATGAGCCAAA TTTCGACCAA 
ACTGGAATGG GGATGACATC ATCTGGGGCA GGGTTAGGCT ACGACCGTGG TCAATTTGTG 
TCTGGCCATG GCCCCCCGCA CGGTATGCTG CGACAGAAGT CTATCGGGGT TCCTGAGGAG 
GAGAAGCAGT TTCTTCATCC TCCAGCCATG AAGTTTGCCC GTAGTCTTTC AGTACCTGGC 
CCAGATGAAA TCCCTCCTCC ACCTACTACA GCACCCCCTG AGCCCCCTTT TTCCTCAGCT 
CCTCCGCTTG GCTTTAGGGG CAAGACATCT CAACAGCCGT CTGTTTCTAT GTCTCAGTCT 
ACTTCCTCTG CTGCACATTA TCAACTCTAC TCACAGTCAG TACATGTTAC TCACGGACCA 
AGGGACAGGT CCCCTGGACC ACCTACTGGG TCTGGAACTG GGGACCTTGC TTATTCCCAT 
GCCCAAGCCC ACCCCTCTGT ACCAAGTAGG ACTGCCTCCA GGAAGGTGAT TGGGTACACA 
GGAGATCTTG CAGGGGCTGG AGGAGTGGCT GGGGCCAAAG CTGCAGCATT GAGAAGGGGT 
TACAGCAATG CAGTCCCACC CACCAGTGTC GCTACTGTGA TTCCACAACA GCAGCAGACT 
ACTCAGAACC AACCTTCCAG AATGGACCGG AGTGGAATGG GGGTAGGGGC TGGTGGTGTA 
CCAAAAGGCG GTGCTCGTAG AGGAAAGGGG CCCTTGGTGA AACAGTCAAA GGTGGAAGAC 
CTGCGTCAAG GTCAAGACAC TCTAGAAGCA AAGGTGTCAG TGGAGAAGAG CTCTATTCCA 
ATCCCAACTA TCATTGTTAA AGCCCCTTCC ACCAGCAGTA GTGGACGCAG TAGCCAAGGC 
AGCAGTGTGG AGGCTGAACC ACCGCCCCCA GGAGAAAGTG ACAGTTCTAA ACCAGAACCT 
GTTGTTCCCC CACCTGCACC AACCGTGCCA CCACCAGCCC CTCCATCTAT ACCTGCTCCT 
CCACCCCCAT CACTGCCATC ACTTCGTACT CAAGAGAGTC TGGACTTCAC CAGCCAGTTT 
GGTGCTGCCA TTGTAGGGGC AGCTCGCAGG GACAGAGAAC GATTTCATGA AGCACGACGC 
AAGAGTGCCT CCTTTTTTCT TTCTGCTGAG GAAGAGTTGA GTGGAGCTGG AGGAGCAGGA 
GGGCGGACTC AGACTTCTCA ACAGCAGCTG AGTTCCCAGC CTTCACCACG CCTGCGACCA 
TCCAAATCTA TAGATGAAGG CATGTTCTCA GGTGATACTT TTATCCATCA CACACGCAGT 
ATGCCTCCTG CATTTGGCTT GCCTGAGTAC TCCTCACCTG CTCCTCAAGG GGATTACCAG 
CCTAAGTCTG TACCTGCTGA CTTCTATGGG GCAGGATCCA GACAACAGCA GCAGCAGCAG 
CAGCAGCAAG CCCCCACCAC AACTTTTATT CACCCACTAA CTGGAAAAGT ACTGGACCCT 
TCATCCCCTC TAGGCTTGGC TTTGGCTGCA CGGGAACGTG CCTTGAAGGA TGATGGACGT 
ATACGTAGGG AAAGGACCAC TGAACATCAT TTTACCCGTC AGTTGTCAAG CATTGGGGCC 
TTTTCCTCAT CCACTTCACA GCCAACATCA CACATTTCCT ACCCAATGGC CTCGTCTTCC 
TCTGCAACCT CCTCTGCTGC TCAATCCACA AACACCTCTT CATATCTTGT TACCTCCTCA 
TCATTAGCTG CCACCACCAC ATCCACTCGG CCTCCATCTC CTAGGATCCT GCGTGGAGCA 
GGGAGTAGCT GGAGTGAAGA AGCTGGGGTT GGAGAGCGTG CGGATAGGGA AGCAGCTGGC 
ACCTCTGCTC CCAGAGAAGG CCTAAGGGTT CGTTTCTCCG AGGATAAAAC TGTCCACACA 
CACCACTATC AATCTCAGCA ATATCAGCCC ACCTTTAAAG AGCGTGAAAG AGAACGTCAA 
AGAGAGAGGT CAAGAGAAAG AGAGAGGGAA AGAGAGAGGG AAAGAGAAAT AGAAAAAGCT 
CAAACCCAGT ATCAGCAACA GTCATCACAA CCAATGACCC AGCAGCCTCG ACGCCCCTCA 
TTCTTGAGGA TGGAGAGTGA AGCTGGAGCG TACATTCTTT CATTAACGCC TCCATCTCCT 
CCTCCAACAG CAGCCCCTAT GCGTCCTACA GCAGGAGCCA CAGGTGAAAG TGGAAGTGGT 
ACAAGCCTGA TGGTTCTGCC TCCCCCAGCT CCCTCAGTAG ATGCAGATGA TGAGTTTGTG 
TTTGCTGATC CTCTTCCTCC ACCTCTAGAG TTTGCCAATA GTTTTGATCG TGGTTTGGGT 
TACTCTGCGA TGATTTCTCA CAATGTTGCA GCCCGTCAGC AGCGTCATCC TCCACCACCC 
CCTCCCCCAC CACCCCCGCC CACTTCGAAA GATTCCTTTA TGACAGGCTT TCCAGCTCAC 
ACACCCCTAC CGTCACCTCA GGCAGGAGAT TCCACAACTT CCAGTCTTAC CTCCTATGAC 
AGTGAGGTTG CAAACCTAAC ACAATCTGCC CCATCGCCCT CCCCATCTTC TCCCAACCCA 
CTACCACCTC ACTCCCCCTC CACCCTTCAG ACATCTGGAC CACCTCCACC TCCCTCTGTA 
TCACCTCCTC CACCACCCAA CAGCTACCAT AGACCTTTTG GCTTATCTCA GTCACAACAT 
TTATACAACA GTACACACAC AACTGGACGC TCCTCCTCTC CCACCCCACC TCTCTCTATT 
CCAGCTCCAC CTGCCTACCG GGGGCCACCA ACAGCATCAT CCACCCCTGC AACTTCTGTA 
TCAATCAGAG GTGCCTCAAC TGTGACCGCA AGCTGTTCCA CAACTGCTTC TACAACTGTC 
ACCTCTCCTA GTACTACTGC TGCTGCCACA CCACACACAC GCATAGCAAC TGTCTCCACA 
GTAGCTACAG GGGGTAGTAG GCGTCCCAGT GCAGAGCATC ATCCACCACC TAGTGCAACC 
AAGAGTGGTG AGTCCCAGGA GACAGTGGTA GATTCTGGGA TTGAGGAGCT GGACAGCCGA 
AGCAGCAGTG ATCATCACTT AGACAGCATT CTTGCACTCA GTGGTAGTGG GGTCAGAGAG 
AGGTTGGAAC GAGCAGGGAT GAGTAGCATA GGTGGAATTG GAGGGAGAGA TGGTGCAACT 
GAGGTTGACA GGACAAGTGG GGCAGACTTT TTAGAGTCTT ACATGAGCTA CCTTGATGGG 
CAGACTTTTG AAATGCACAA TGCCAAAGCC GCTAGCACTG CATCCACCTA CCCAAAAACA 
CAGAGGTACA GAGAAGGCAC CCGGGCTGGA GATCTTCGAC GCCAGACCAG CACAGCACCT 
CCTGCATACC ACCGTCGTAG AGAGGAGGAA GAGGAAGAGG ATGAGGCCGA GGAGGTAGAG 
GAGGAGGATT TGAGTGCCCG TGATGGCTAT GGGATGAGGG AAGGGCCAGG GATTGCTCGC 
TCCTCTGTAA TGTACTTTGA CCGGCCCCGC ACTCCAGAGA TAAAACCACT GTGGGGAGAT 
GGGTCGACTG GAGCATCACA GACCATGGGG GAGGGAGGGG GCCAGATAGT GGGAACATAT 
GTGGAGCCAC GTAAACTACA TCCTCCCATG ACTGGTATGA AAGCAAACAT CATTAATGAA 
CTAAGCACCA AATTACAGCA GAGAAGTAAA GGAACAGAGA ACTGGGGGGC CCAGAGATCA 
TTGAGCAGAC ATAGGTTCTC AGAGGACTCA ACAGGTGCAC TGAGTCCTCC CTCTGTCCGC 
TCTAGCTCCC CATCTCCAGT TCAGCTCTCT CAGCCTGCCT TGTCTCCCTC TCCCACTCCT 
TCCTCCCAGC CACTTTACTC AAACTGGGCT CGATCTCCTT CCCCTCAGCC CCCTGCTGCC 
TCATCCCAGC CCCCTCCCCG ATCCCATTCA CCTGTTTCCC CTTCATCATA TTCACCATAC 
CCCACCTCAC CCAAACACCG GCCTGTTTAT CGCACCAAAG CTCTGGAGTT CCAATTTATT 
CCCAGTCGAG AGTCACGAAA GTCAGAGTCG CATCATGCCC AGCGCAGACG AGCACCCAGC 
CCTTTAATTT CTCCATCTGA CCGTCCCAAG CTTGGCCCTC CCCGCCCCTC TTCCCTTCCT 
ATTCTCCCCA CTACACCCCT CTATAGCAGC CTGTATGACC TTCGTGGTTC TATTACTCCA 
CCCTCACCTG CCAATCCCCT TGGGGACCCC TACTTTTCCC CCTCCCCCCC TCTATTTGCC 
CCCTCGGGTG CTCCCCCTCC TCCAAATTCC TCTCTGGTGG TCTCACGGTC CCTGTCACCC 
ACTCACTTCC TGTCAGGGAC TTCATCCCCA CCTCTCCACC CCATTCCTCC ACCCACTTGC 
CTGAGTTACC CCCATCTTCC ACCCCCCTCA CCCACAAAGC CCTTTGCCTC AAAACCTCTC 
CCATACTGGA CCAAGTATGA TGTTGCAGAT TGGCTGGGCT ACCTGAACCT GGGTGAACAC 
AGGGAGCGAT TCTTAGACAA TGAAATTGAT GGCACGCACC TGCCCTCTCT TACCAAGGAT 
GACTACCTGG ACCTGGGTGT GACCCGAGTT GGCCATCGCA TGAACATAGA GAGGGCGCTC 
AGGAGAGTTA TGGACAGAAC CATCAGTAAA GATCTCATCC CCTCCATCTC CATATCTTTC 
ATCTGTTCAT CTTCTAAGAG TGTGGCATCT TCATTCATCA TACTGCTCAC TTTACCTTAC 
TCATACTGGA CAAAAAGATT GAGGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_009299490.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297765.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X5, mRNA.

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CloneID	ODa45593
Clone ID Related Accession (Same CDS sequence)	XM_009299490.2
Accession Version	XM_009299490.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299490.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGTG 
GACAGAGCCT CTGCCCCATG GAAAAAAAAA TCAGAGTTCG AATCTTCACA AGCTACAGAG 
AAGAAGAGGA CAGTTTATCA GATGGCTTTG AATAAACTGG ATGAGATTTT AGCAGCAGCA 
CAGCAGACCA TTAATACAAA TGAGGCTCCA GGGCCCCGGG TTCAGGGGGT AAAAAGAGAA 
CGGGGCAGAG GCTTTTATAA TGAGCCAAAT TTCGACCAAA CTGGAATGGG GATGACATCA 
TCTGGGGCAG GGTTAGGCTA CGACCGTGGT CAATTTGTGT CTGGCCATGG CCCCCCGCAC 
GGTATGCTGC GACAGAAGTC TATCGGGGTT CCTGAGGAGG AGAAGCAGTT TCTTCATCCT 
CCAGCCATGA AGTTTGCCCG TAGTCTTTCA GTACCTGGCC CAGATGAAAT CCCTCCTCCA 
CCTACTACAG CACCCCCTGA GCCCCCTTTT TCCTCAGCTC CTCCGCTTGG CTTTAGGGGC 
AAGACATCTC AACAGCCGTC TGTTTCTATG TCTCAGTCTA CTTCCTCTGC TGCACATTAT 
CAACTCTACT CACAGTCAGT ACATGTTACT CACGGACCAA GGGACAGGTC CCCTGGACCA 
CCTACTGGGT CTGGAACTGG GGACCTTGCT TATTCCCATG CCCAAGCCCA CCCCTCTGTA 
CCAAGTAGGA CTGCCTCCAG GAAGGTGATT GGGTACACAG GAGATCTTGC AGGGGCTGGA 
GGAGTGGCTG GGGCCAAAGC TGCAGCATTG AGAAGGGGTT ACAGCAATGC AGTCCCACCC 
ACCAGTGTCG CTACTGTGAT TCCACAACAG CAGCAGACTA CTCAGAACCA ACCTTCCAGA 
ATGGACCGGA GTGGAATGGG GGTAGGGGCT GGTGGTGTAC CAAAAGGCGG TGCTCGTAGA 
GGAAAGGGGC CCTTGGTGAA ACAGTCAAAG GTGGAAGACC TGCGTCAAGG TCAAGACACT 
CTAGAAGCAA AGGTGTCAGT GGAGAAGAGC TCTATTCCAA TCCCAACTAT CATTGTTAAA 
GCCCCTTCCA CCAGCAGTAG TGGACGCAGT AGCCAAGGCA GCAGTGTGGA GGCTGAACCA 
CCGCCCCCAG GAGAAAGTGA CAGTTCTAAA CCAGAACCTG TTGTTCCCCC ACCTGCACCA 
ACCGTGCCAC CACCAGCCCC TCCATCTATA CCTGCTCCTC CACCCCCATC ACTGCCATCA 
CTTCGTACTC AAGAGAGTCT GGACTTCACC AGCCAGTTTG GTGCTGCCAT TGTAGGGGCA 
GCTCGCAGGG ACAGAGAACG ATTTCATGAA GCACGACGCA AGAGTGCCTC CTTTTTTCTT 
TCTGCTGAGG AAGAGTTGAG TGGAGCTGGA GGAGCAGGAG GGCGGACTCA GACTTCTCAA 
CAGCAGCTGA GTTCCCAGCC TTCACCACGC CTGCGACCAT CCAAATCTAT AGATGAAGGC 
ATGTTCTCAG GTGATACTTT TATCCATCAC ACACGCAGTA TGCCTCCTGC ATTTGGCTTG 
CCTGAGTACT CCTCACCTGC TCCTCAAGGG GATTACCAGC CTAAGTCTGT ACCTGCTGAC 
TTCTATGGGG CAGGATCCAG ACAACAGCAG CAGCAGCAGC AGCAGCAAGC CCCCACCACA 
ACTTTTATTC ACCCACTAAC TGGAAAAGTA CTGGACCCTT CATCCCCTCT AGGCTTGGCT 
TTGGCTGCAC GGGAACGTGC CTTGAAGGAT GATGGACGTA TACGTAGGGA AAGGACCACT 
GAACATCATT TTACCCGTCA GTTGTCAAGC ATTGGGGCCT TTTCCTCATC CACTTCACAG 
CCAACATCAC ACATTTCCTA CCCAATGGCC TCGTCTTCCT CTGCAACCTC CTCTGCTGCT 
CAATCCACAA ACACCTCTTC ATATCTTGTT ACCTCCTCAT CATTAGCTGC CACCACCACA 
TCCACTCGGC CTCCATCTCC TAGGATCCTG CGTGGAGCAG GGAGTAGCTG GAGTGAAGAA 
GCTGGGGTTG GAGAGCGTGC GGATAGGGAA GCAGCTGGCA CCTCTGCTCC CAGAGAAGGC 
CTAAGGGTTC GTTTCTCCGA GGATAAAACT GTCCACACAC ACCACTATCA ATCTCAGCAA 
TATCAGCCCA CCTTTAAAGA GCGTGAAAGA GAACGTCAAA GAGAGAGGTC AAGAGAAAGA 
GAGAGGGAAA GAGAGAGGGA AAGAGAAATA GAAAAAGCTC AAACCCAGTA TCAGCAACAG 
TCATCACAAC CAATGACCCA GCAGCCTCGA CGCCCCTCAT TCTTGAGGAT GGAGAGTGAA 
GCTGGAGCGT ACATTCTTTC ATTAACGCCT CCATCTCCTC CTCCAACAGC AGCCCCTATG 
CGTCCTACAG CAGGAGCCAC AGGTGAAAGT GGAAGTGGTA CAAGCCTGAT GGTTCTGCCT 
CCCCCAGCTC CCTCAGTAGA TGCAGATGAT GAGTTTGTGT TTGCTGATCC TCTTCCTCCA 
CCTCTAGAGT TTGCCAATAG TTTTGATCGT GGTTTGGGTT ACTCTGCGAT GATTTCTCAC 
AATGTTGCAG CCCGTCAGCA GCGTCATCCT CCACCACCCC CTCCCCCACC ACCCCCGCCC 
ACTTCGAAAG ATTCCTTTAT GACAGGCTTT CCAGCTCACA CACCCCTACC GTCACCTCAG 
GCAGGAGATT CCACAACTTC CAGTCTTACC TCCTATGACA GTGAGGTTGC AAACCTAACA 
CAATCTGCCC CATCGCCCTC CCCATCTTCT CCCAACCCAC TACCACCTCA CTCCCCCTCC 
ACCCTTCAGA CATCTGGACC ACCTCCACCT CCCTCTGTAT CACCTCCTCC ACCACCCAAC 
AGCTACCATA GACCTTTTGG CTTATCTCAG TCACAACATT TATACAACAG TACACACACA 
ACTGGACGCT CCTCCTCTCC CACCCCACCT CTCTCTATTC CAGCTCCACC TGCCTACCGG 
GGGCCACCAA CAGCATCATC CACCCCTGCA ACTTCTGTAT CAATCAGAGG TGCCTCAACT 
GTGACCGCAA GCTGTTCCAC AACTGCTTCT ACAACTGTCA CCTCTCCTAG TACTACTGCT 
GCTGCCACAC CACACACACG CATAGCAACT GTCTCCACAG TAGCTACAGG GGGTAGTAGG 
CGTCCCAGTG CAGAGCATCA TCCACCACCT AGTGCAACCA AGAGTGGTGA GTCCCAGGAG 
ACAGTGGTAG ATTCTGGGAT TGAGGAGCTG GACAGCCGAA GCAGCAGTGA TCATCACTTA 
GACAGCATTC TTGCACTCAG TGGTAGTGGG GTCAGAGAGA GGTTGGAACG AGCAGGGATG 
AGTAGCATAG GTGGAATTGG AGGGAGAGAT GGTGCAACTG AGGTTGACAG GACAAGTGGG 
GCAGACTTTT TAGAGTCTTA CATGAGCTAC CTTGATGGGC AGACTTTTGA AATGCACAAT 
GCCAAAGCCG CTAGCACTGC ATCCACCTAC CCAAAAACAC AGAGGTACAG AGAAGGCACC 
CGGGCTGGAG ATCTTCGACG CCAGACCAGC ACAGCACCTC CTGCATACCA CCGTCGTAGA 
GAGGAGGAAG AGGAAGAGGA TGAGGCCGAG GAGGTAGAGG AGGAGGATTT GAGTGCCCGT 
GATGGCTATG GGATGAGGGA AGGGCCAGGG ATTGCTCGCT CCTCTGTAAT GTACTTTGAC 
CGGCCCCGCA CTCCAGAGAT AAAACCACTG TGGGGAGATG GGTCGACTGG AGCATCACAG 
ACCATGGGGG AGGGAGGGGG CCAGATAGTG GGAACATATG TGGAGCCACG TAAACTACAT 
CCTCCCATGA CTGGTATGAA AGCAAACATC ATTAATGAAC TAAGCACCAA ATTACAGCAG 
AGAAGTAAAG GAACAGAGAA CTGGGGGGCC CAGAGATCAT TGAGCAGACA TAGACACAGG 
TTCTCAGAGG ACTCAACAGG TGCACTGAGT CCTCCCTCTG TCCGCTCTAG CTCCCCATCT 
CCAGTTCAGC TCTCTCAGCC TGCCTTGTCT CCCTCTCCCA CTCCTTCCTC CCAGCCACTT 
TACTCAAACT GGGCTCGATC TCCTTCCCCT CAGCCCCCTG CTGCCTCATC CCAGCCCCCT 
CCCCGATCCC ATTCACCTGT TTCCCCTTCA TCATATTCAC CATACCCCAC CTCACCCAAA 
CACCGGCCTG TTTATCGCAC CAAAGCTCTG GAGTTCCAAT TTATTCCCAG TCGAGAGTCA 
CGAAAGTCAG AGTCGCATCA TGCCCAGCGC AGACGAGCAC CCAGCCCTTT AATTTCTCCA 
TCTGACCGTC CCAAGCTTGG CCCTCCCCGC CCCTCTTCCC TTCCTATTCT CCCCACTACA 
CCCCTCTATA GCAGCCTGTA TGACCTTCGT GGTTCTATTA CTCCACCCTC ACCTGCCAAT 
CCCCTTGGGG ACCCCTACTT TTCCCCCTCC CCCCCTCTAT TTGCCCCCTC GGGTGCTCCC 
CCTCCTCCAA ATTCCTCTCT GGTGGTCTCA CGGTCCCTGT CACCCACTCA CTTCCTGTCA 
GGGACTTCAT CCCCACCTCT CCACCCCATT CCTCCACCCA CTTGCCTGAG TTACCCCCAT 
CTTCCACCCC CCTCACCCAC AAAGCCCTTT GCCTCAAAAC CTCTCCCATA CTGGACCAAG 
TATGATGTTG CAGATTGGCT GGGCTACCTG AACCTGGGTG AACACAGGGA GCGATTCTTA 
GACAATGAAA TTGATGGCAC GCACCTGCCC TCTCTTACCA AGGATGACTA CCTGGACCTG 
GGTGTGACCC GAGTTGGCCA TCGCATGAAC ATAGAGAGGG CGCTCAGGAG AGTTATGGAC 
AGAACCATCA GTAAAGATCT CATCCCCTCC ATCTCCATAT CTTTCATCTG TTCATCTTCT 
AAGAGTGTGG CATCTTCATT CATCATACTG CTCACTTTAC CTTACTCATA CTGGACAAAA 
AGATTGAGGA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297765.1
CDS	825..8279
Translation	MTMPLSPLSSDEEQQRMLGNSQHLYPGAEEEEEEEEEMDDDGMDEEGEEENGELEMDDEEEVDDEDDIRRGPGHLRGGREEGHRQKGTMAGRLPGDRPIRPGNPGHAANPYSSNPGPTHQTSTNQQQQQPQRKLRERSSSNAPDDTHVALMVFRIGIPDIKQTKCLRFNPDATVWKAKQQVMCSLTESLRDVLNYGLFQPATDGHDAKFLEEERPLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKASLKKFMDYIQTSAVEKVAKLIDKGLDPNYHDPDTGETPLTLAVQTEPGGSEIIRVLVMGGAHIDFRARDGLTPLHKAVRAHAHTALLTLLSLGASSDYKDSRGLTPLYHTVLIGGDTSCCETLLYHRARLGLRDENGWDETHQACQNGNSQHLEHLLFYGADSSSQNASGNTALHICALYNKESCARILLYRGAKKDTKNNSGQNSFQVAVMSGHFELGEIIKNHRESDVVPFLESPKYAPQRRESSRTLGLPHPHPFLRANSDNSMNVPDWIAFPNAAGSSIVSVQGLKHGGTLRSSSSPRGARTRSPSRGRTGDRDDRSRQTRGRQGAGSISSQGTASGQRRRLYSAVPGRVFVATRSHSAQGDREISFNKGDKVKVLSVGEGGYWEGTVRGRTGWFPSDCVEEVALRSLESRSESRSERAKRLFRHYTVGSYDSFDAPSDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMVDRASAPWKKKSEFESSQATEKKRTVYQMALNKLDEILAAAQQTINTNEAPGPRVQGVKRERGRGFYNEPNFDQTGMGMTSSGAGLGYDRGQFVSGHGPPHGMLRQKSIGVPEEEKQFLHPPAMKFARSLSVPGPDEIPPPPTTAPPEPPFSSAPPLGFRGKTSQQPSVSMSQSTSSAAHYQLYSQSVHVTHGPRDRSPGPPTGSGTGDLAYSHAQAHPSVPSRTASRKVIGYTGDLAGAGGVAGAKAAALRRGYSNAVPPTSVATVIPQQQQTTQNQPSRMDRSGMGVGAGGVPKGGARRGKGPLVKQSKVEDLRQGQDTLEAKVSVEKSSIPIPTIIVKAPSTSSSGRSSQGSSVEAEPPPPGESDSSKPEPVVPPPAPTVPPPAPPSIPAPPPPSLPSLRTQESLDFTSQFGAAIVGAARRDRERFHEARRKSASFFLSAEEELSGAGGAGGRTQTSQQQLSSQPSPRLRPSKSIDEGMFSGDTFIHHTRSMPPAFGLPEYSSPAPQGDYQPKSVPADFYGAGSRQQQQQQQQQAPTTTFIHPLTGKVLDPSSPLGLALAARERALKDDGRIRRERTTEHHFTRQLSSIGAFSSSTSQPTSHISYPMASSSSATSSAAQSTNTSSYLVTSSSLAATTTSTRPPSPRILRGAGSSWSEEAGVGERADREAAGTSAPREGLRVRFSEDKTVHTHHYQSQQYQPTFKERERERQRERSREREREREREREIEKAQTQYQQQSSQPMTQQPRRPSFLRMESEAGAYILSLTPPSPPPTAAPMRPTAGATGESGSGTSLMVLPPPAPSVDADDEFVFADPLPPPLEFANSFDRGLGYSAMISHNVAARQQRHPPPPPPPPPPPTSKDSFMTGFPAHTPLPSPQAGDSTTSSLTSYDSEVANLTQSAPSPSPSSPNPLPPHSPSTLQTSGPPPPPSVSPPPPPNSYHRPFGLSQSQHLYNSTHTTGRSSSPTPPLSIPAPPAYRGPPTASSTPATSVSIRGASTVTASCSTTASTTVTSPSTTAAATPHTRIATVSTVATGGSRRPSAEHHPPPSATKSGESQETVVDSGIEELDSRSSSDHHLDSILALSGSGVRERLERAGMSSIGGIGGRDGATEVDRTSGADFLESYMSYLDGQTFEMHNAKAASTASTYPKTQRYREGTRAGDLRRQTSTAPPAYHRRREEEEEEDEAEEVEEEDLSARDGYGMREGPGIARSSVMYFDRPRTPEIKPLWGDGSTGASQTMGEGGGQIVGTYVEPRKLHPPMTGMKANIINELSTKLQQRSKGTENWGAQRSLSRHRHRFSEDSTGALSPPSVRSSSPSPVQLSQPALSPSPTPSSQPLYSNWARSPSPQPPAASSQPPPRSHSPVSPSSYSPYPTSPKHRPVYRTKALEFQFIPSRESRKSESHHAQRRRAPSPLISPSDRPKLGPPRPSSLPILPTTPLYSSLYDLRGSITPPSPANPLGDPYFSPSPPLFAPSGAPPPPNSSLVVSRSLSPTHFLSGTSSPPLHPIPPPTCLSYPHLPPPSPTKPFASKPLPYWTKYDVADWLGYLNLGEHRERFLDNEIDGTHLPSLTKDDYLDLGVTRVGHRMNIERALRRVMDRTISKDLIPSISISFICSSSKSVASSFIILLTLPYSYWTKRLRN

Target ORF information:

RefSeq Version	XM_009299490.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299490.2

ORF Insert Sequence:

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGTGCTGAG TGTGGGTGAA 
GGAGGCTATT GGGAAGGGAC GGTTCGAGGA CGCACAGGCT GGTTCCCCTC AGACTGCGTG 
GAGGAAGTTG CTCTCCGTAG CCTGGAATCT CGTTCAGAGA GCCGCAGTGA GAGAGCCAAG 
AGACTCTTCC GGCATTATAC TGTTGGATCC TACGACAGCT TCGATGCGCC CAGTGACTAC 
ATAATCAAGG AGAAGACAGT CCTCCTGCAG AAAAAAGACA ATGAAGGTTT TGGCTTTGTG 
CTGAGAGGAG CCAAGGCTCA GACCCCTATA GAAGAGTTCA CTCCCACCCC AGCCTTTCCA 
GCACTGCAGT ACCTTGAGTC TGTCGACGAG GGAGGCGTGG CCTGGAGAGC TGGTCTGCGA 
ATGGGAGACT TCCTTATAGA GGTGAATGGT CAGAATGTGG TTAAGGTGGG ACACAGGCAG 
GTGGTCAATA TGATCAGGCA AGGCGGTAAC AGTCTCATGG TCAAGGTTGT CATGGTTACT 
CGAAATCCTG ATATGGAGGA AGGGGTCAGA AAGAAAATCC CACAGCAGAG TAAAAGACTG 
AGCACACCGG CTATCGCTCT GCGCTCCAAA TCCATGACAT CAGAGCTGGA AGAGATGGTG 
GACAGAGCCT CTGCCCCATG GAAAAAAAAA TCAGAGTTCG AATCTTCACA AGCTACAGAG 
AAGAAGAGGA CAGTTTATCA GATGGCTTTG AATAAACTGG ATGAGATTTT AGCAGCAGCA 
CAGCAGACCA TTAATACAAA TGAGGCTCCA GGGCCCCGGG TTCAGGGGGT AAAAAGAGAA 
CGGGGCAGAG GCTTTTATAA TGAGCCAAAT TTCGACCAAA CTGGAATGGG GATGACATCA 
TCTGGGGCAG GGTTAGGCTA CGACCGTGGT CAATTTGTGT CTGGCCATGG CCCCCCGCAC 
GGTATGCTGC GACAGAAGTC TATCGGGGTT CCTGAGGAGG AGAAGCAGTT TCTTCATCCT 
CCAGCCATGA AGTTTGCCCG TAGTCTTTCA GTACCTGGCC CAGATGAAAT CCCTCCTCCA 
CCTACTACAG CACCCCCTGA GCCCCCTTTT TCCTCAGCTC CTCCGCTTGG CTTTAGGGGC 
AAGACATCTC AACAGCCGTC TGTTTCTATG TCTCAGTCTA CTTCCTCTGC TGCACATTAT 
CAACTCTACT CACAGTCAGT ACATGTTACT CACGGACCAA GGGACAGGTC CCCTGGACCA 
CCTACTGGGT CTGGAACTGG GGACCTTGCT TATTCCCATG CCCAAGCCCA CCCCTCTGTA 
CCAAGTAGGA CTGCCTCCAG GAAGGTGATT GGGTACACAG GAGATCTTGC AGGGGCTGGA 
GGAGTGGCTG GGGCCAAAGC TGCAGCATTG AGAAGGGGTT ACAGCAATGC AGTCCCACCC 
ACCAGTGTCG CTACTGTGAT TCCACAACAG CAGCAGACTA CTCAGAACCA ACCTTCCAGA 
ATGGACCGGA GTGGAATGGG GGTAGGGGCT GGTGGTGTAC CAAAAGGCGG TGCTCGTAGA 
GGAAAGGGGC CCTTGGTGAA ACAGTCAAAG GTGGAAGACC TGCGTCAAGG TCAAGACACT 
CTAGAAGCAA AGGTGTCAGT GGAGAAGAGC TCTATTCCAA TCCCAACTAT CATTGTTAAA 
GCCCCTTCCA CCAGCAGTAG TGGACGCAGT AGCCAAGGCA GCAGTGTGGA GGCTGAACCA 
CCGCCCCCAG GAGAAAGTGA CAGTTCTAAA CCAGAACCTG TTGTTCCCCC ACCTGCACCA 
ACCGTGCCAC CACCAGCCCC TCCATCTATA CCTGCTCCTC CACCCCCATC ACTGCCATCA 
CTTCGTACTC AAGAGAGTCT GGACTTCACC AGCCAGTTTG GTGCTGCCAT TGTAGGGGCA 
GCTCGCAGGG ACAGAGAACG ATTTCATGAA GCACGACGCA AGAGTGCCTC CTTTTTTCTT 
TCTGCTGAGG AAGAGTTGAG TGGAGCTGGA GGAGCAGGAG GGCGGACTCA GACTTCTCAA 
CAGCAGCTGA GTTCCCAGCC TTCACCACGC CTGCGACCAT CCAAATCTAT AGATGAAGGC 
ATGTTCTCAG GTGATACTTT TATCCATCAC ACACGCAGTA TGCCTCCTGC ATTTGGCTTG 
CCTGAGTACT CCTCACCTGC TCCTCAAGGG GATTACCAGC CTAAGTCTGT ACCTGCTGAC 
TTCTATGGGG CAGGATCCAG ACAACAGCAG CAGCAGCAGC AGCAGCAAGC CCCCACCACA 
ACTTTTATTC ACCCACTAAC TGGAAAAGTA CTGGACCCTT CATCCCCTCT AGGCTTGGCT 
TTGGCTGCAC GGGAACGTGC CTTGAAGGAT GATGGACGTA TACGTAGGGA AAGGACCACT 
GAACATCATT TTACCCGTCA GTTGTCAAGC ATTGGGGCCT TTTCCTCATC CACTTCACAG 
CCAACATCAC ACATTTCCTA CCCAATGGCC TCGTCTTCCT CTGCAACCTC CTCTGCTGCT 
CAATCCACAA ACACCTCTTC ATATCTTGTT ACCTCCTCAT CATTAGCTGC CACCACCACA 
TCCACTCGGC CTCCATCTCC TAGGATCCTG CGTGGAGCAG GGAGTAGCTG GAGTGAAGAA 
GCTGGGGTTG GAGAGCGTGC GGATAGGGAA GCAGCTGGCA CCTCTGCTCC CAGAGAAGGC 
CTAAGGGTTC GTTTCTCCGA GGATAAAACT GTCCACACAC ACCACTATCA ATCTCAGCAA 
TATCAGCCCA CCTTTAAAGA GCGTGAAAGA GAACGTCAAA GAGAGAGGTC AAGAGAAAGA 
GAGAGGGAAA GAGAGAGGGA AAGAGAAATA GAAAAAGCTC AAACCCAGTA TCAGCAACAG 
TCATCACAAC CAATGACCCA GCAGCCTCGA CGCCCCTCAT TCTTGAGGAT GGAGAGTGAA 
GCTGGAGCGT ACATTCTTTC ATTAACGCCT CCATCTCCTC CTCCAACAGC AGCCCCTATG 
CGTCCTACAG CAGGAGCCAC AGGTGAAAGT GGAAGTGGTA CAAGCCTGAT GGTTCTGCCT 
CCCCCAGCTC CCTCAGTAGA TGCAGATGAT GAGTTTGTGT TTGCTGATCC TCTTCCTCCA 
CCTCTAGAGT TTGCCAATAG TTTTGATCGT GGTTTGGGTT ACTCTGCGAT GATTTCTCAC 
AATGTTGCAG CCCGTCAGCA GCGTCATCCT CCACCACCCC CTCCCCCACC ACCCCCGCCC 
ACTTCGAAAG ATTCCTTTAT GACAGGCTTT CCAGCTCACA CACCCCTACC GTCACCTCAG 
GCAGGAGATT CCACAACTTC CAGTCTTACC TCCTATGACA GTGAGGTTGC AAACCTAACA 
CAATCTGCCC CATCGCCCTC CCCATCTTCT CCCAACCCAC TACCACCTCA CTCCCCCTCC 
ACCCTTCAGA CATCTGGACC ACCTCCACCT CCCTCTGTAT CACCTCCTCC ACCACCCAAC 
AGCTACCATA GACCTTTTGG CTTATCTCAG TCACAACATT TATACAACAG TACACACACA 
ACTGGACGCT CCTCCTCTCC CACCCCACCT CTCTCTATTC CAGCTCCACC TGCCTACCGG 
GGGCCACCAA CAGCATCATC CACCCCTGCA ACTTCTGTAT CAATCAGAGG TGCCTCAACT 
GTGACCGCAA GCTGTTCCAC AACTGCTTCT ACAACTGTCA CCTCTCCTAG TACTACTGCT 
GCTGCCACAC CACACACACG CATAGCAACT GTCTCCACAG TAGCTACAGG GGGTAGTAGG 
CGTCCCAGTG CAGAGCATCA TCCACCACCT AGTGCAACCA AGAGTGGTGA GTCCCAGGAG 
ACAGTGGTAG ATTCTGGGAT TGAGGAGCTG GACAGCCGAA GCAGCAGTGA TCATCACTTA 
GACAGCATTC TTGCACTCAG TGGTAGTGGG GTCAGAGAGA GGTTGGAACG AGCAGGGATG 
AGTAGCATAG GTGGAATTGG AGGGAGAGAT GGTGCAACTG AGGTTGACAG GACAAGTGGG 
GCAGACTTTT TAGAGTCTTA CATGAGCTAC CTTGATGGGC AGACTTTTGA AATGCACAAT 
GCCAAAGCCG CTAGCACTGC ATCCACCTAC CCAAAAACAC AGAGGTACAG AGAAGGCACC 
CGGGCTGGAG ATCTTCGACG CCAGACCAGC ACAGCACCTC CTGCATACCA CCGTCGTAGA 
GAGGAGGAAG AGGAAGAGGA TGAGGCCGAG GAGGTAGAGG AGGAGGATTT GAGTGCCCGT 
GATGGCTATG GGATGAGGGA AGGGCCAGGG ATTGCTCGCT CCTCTGTAAT GTACTTTGAC 
CGGCCCCGCA CTCCAGAGAT AAAACCACTG TGGGGAGATG GGTCGACTGG AGCATCACAG 
ACCATGGGGG AGGGAGGGGG CCAGATAGTG GGAACATATG TGGAGCCACG TAAACTACAT 
CCTCCCATGA CTGGTATGAA AGCAAACATC ATTAATGAAC TAAGCACCAA ATTACAGCAG 
AGAAGTAAAG GAACAGAGAA CTGGGGGGCC CAGAGATCAT TGAGCAGACA TAGACACAGG 
TTCTCAGAGG ACTCAACAGG TGCACTGAGT CCTCCCTCTG TCCGCTCTAG CTCCCCATCT 
CCAGTTCAGC TCTCTCAGCC TGCCTTGTCT CCCTCTCCCA CTCCTTCCTC CCAGCCACTT 
TACTCAAACT GGGCTCGATC TCCTTCCCCT CAGCCCCCTG CTGCCTCATC CCAGCCCCCT 
CCCCGATCCC ATTCACCTGT TTCCCCTTCA TCATATTCAC CATACCCCAC CTCACCCAAA 
CACCGGCCTG TTTATCGCAC CAAAGCTCTG GAGTTCCAAT TTATTCCCAG TCGAGAGTCA 
CGAAAGTCAG AGTCGCATCA TGCCCAGCGC AGACGAGCAC CCAGCCCTTT AATTTCTCCA 
TCTGACCGTC CCAAGCTTGG CCCTCCCCGC CCCTCTTCCC TTCCTATTCT CCCCACTACA 
CCCCTCTATA GCAGCCTGTA TGACCTTCGT GGTTCTATTA CTCCACCCTC ACCTGCCAAT 
CCCCTTGGGG ACCCCTACTT TTCCCCCTCC CCCCCTCTAT TTGCCCCCTC GGGTGCTCCC 
CCTCCTCCAA ATTCCTCTCT GGTGGTCTCA CGGTCCCTGT CACCCACTCA CTTCCTGTCA 
GGGACTTCAT CCCCACCTCT CCACCCCATT CCTCCACCCA CTTGCCTGAG TTACCCCCAT 
CTTCCACCCC CCTCACCCAC AAAGCCCTTT GCCTCAAAAC CTCTCCCATA CTGGACCAAG 
TATGATGTTG CAGATTGGCT GGGCTACCTG AACCTGGGTG AACACAGGGA GCGATTCTTA 
GACAATGAAA TTGATGGCAC GCACCTGCCC TCTCTTACCA AGGATGACTA CCTGGACCTG 
GGTGTGACCC GAGTTGGCCA TCGCATGAAC ATAGAGAGGG CGCTCAGGAG AGTTATGGAC 
AGAACCATCA GTAAAGATCT CATCCCCTCC ATCTCCATAT CTTTCATCTG TTCATCTTCT 
AAGAGTGTGG CATCTTCATT CATCATACTG CTCACTTTAC CTTACTCATA CTGGACAAAA 
AGATTGAGGA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_009299491.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297766.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X6, mRNA.

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CloneID	ODa45594
Clone ID Related Accession (Same CDS sequence)	XM_009299491.2
Accession Version	XM_009299491.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299491.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGGTGTGGA TAAACGTTGT 
CTGTTGCTGA GTGTGGGTGA AGGAGGCTAT TGGGAAGGGA CGGTTCGAGG ACGCACAGGC 
TGGTTCCCCT CAGACTGCGT GGAGGAAGTT GCTCTCCGTA GCCTGGAATC TCGTTCAGAG 
AGCCGCAGTG AGAGAGCCAA GAGACTCTTC CGGCATTATA CTGTTGGATC CTACGACAGC 
TTCGATGCGC CCAGTGACTA CATAATCAAG GAGAAGACAG TCCTCCTGCA GAAAAAAGAC 
AATGAAGGTT TTGGCTTTGT GCTGAGAGGA GCCAAGGCTC AGACCCCTAT AGAAGAGTTC 
ACTCCCACCC CAGCCTTTCC AGCACTGCAG TACCTTGAGT CTGTCGACGA GGGAGGCGTG 
GCCTGGAGAG CTGGTCTGCG AATGGGAGAC TTCCTTATAG AGGTGAATGG TCAGAATGTG 
GTTAAGGTGG GACACAGGCA GGTGGTCAAT ATGATCAGGC AAGGCGGTAA CAGTCTCATG 
GTCAAGGTTG TCATGGTTAC TCGAAATCCT GATATGGAGG AAGGGGTCAG AAAGAAAATC 
CCACAGCAGA GTAAAAGACT GAGCACACCG GCTATCGCTC TGCGCTCCAA ATCCATGACA 
TCAGAGCTGG AAGAGATGGT GGACAGAGCC TCTGCCCCAT GGAAAAAAAA ATCAGAGTTC 
GAATCTTCAC AAGCTACAGA GAAGAAGAGG ACAGTTTATC AGATGGCTTT GAATAAACTG 
GATGAGATTT TAGCAGCAGC ACAGCAGACC ATTAATACAA ATGAGGCTCC AGGGCCCCGG 
GTTCAGGGGG TAAAAAGAGA ACGGGGCAGA GGCTTTTATA ATGAGCCAAA TTTCGACCAA 
ACTGGAATGG GGATGACATC ATCTGGGGCA GGGTTAGGCT ACGACCGTGG TCAATTTGTG 
TCTGGCCATG GCCCCCCGCA CGGGGTTCCT GAGGAGGAGA AGCAGTTTCT TCATCCTCCA 
GCCATGAAGT TTGCCCGTAG TCTTTCAGTA CCTGGCCCAG ATGAAATCCC TCCTCCACCT 
ACTACAGCAC CCCCTGAGCC CCCTTTTTCC TCAGCTCCTC CGCTTGGCTT TAGGGGCAAG 
ACATCTCAAC AGCCGTCTGT TTCTATGTCT CAGTCTACTT CCTCTGCTGC ACATTATCAA 
CTCTACTCAC AGTCAGTACA TGTTACTCAC GGACCAAGGG ACAGGTCCCC TGGACCACCT 
ACTGGGTCTG GAACTGGGGA CCTTGCTTAT TCCCATGCCC AAGCCCACCC CTCTGTACCA 
AGTAGGACTG CCTCCAGGAA GGTGATTGGG TACACAGGAG ATCTTGCAGG GGCTGGAGGA 
GTGGCTGGGG CCAAAGCTGC AGCATTGAGA AGGGGTTACA GCAATGCAGT CCCACCCACC 
AGTGTCGCTA CTGTGATTCC ACAACAGCAG CAGACTACTC AGAACCAACC TTCCAGAATG 
GACCGGAGTG GAATGGGGGT AGGGGCTGGT GGTGTACCAA AAGGCGGTGC TCGTAGAGGA 
AAGGGGCCCT TGGTGAAACA GTCAAAGGTG GAAGACCTGC GTCAAGGTCA AGACACTCTA 
GAAGCAAAGG TGTCAGTGGA GAAGAGCTCT ATTCCAATCC CAACTATCAT TGTTAAAGCC 
CCTTCCACCA GCAGTAGTGG ACGCAGTAGC CAAGGCAGCA GTGTGGAGGC TGAACCACCG 
CCCCCAGGAG AAAGTGACAG TTCTAAACCA GAACCTGTTG TTCCCCCACC TGCACCAACC 
GTGCCACCAC CAGCCCCTCC ATCTATACCT GCTCCTCCAC CCCCATCACT GCCATCACTT 
CGTACTCAAG AGAGTCTGGA CTTCACCAGC CAGTTTGGTG CTGCCATTGT AGGGGCAGCT 
CGCAGGGACA GAGAACGATT TCATGAAGCA CGACGCAAGA GTGCCTCCTT TTTTCTTTCT 
GCTGAGGAAG AGTTGAGTGG AGCTGGAGGA GCAGGAGGGC GGACTCAGAC TTCTCAACAG 
CAGCTGAGTT CCCAGCCTTC ACCACGCCTG CGACCATCCA AATCTATAGA TGAAGGCATG 
TTCTCAGGTG ATACTTTTAT CCATCACACA CGCAGTATGC CTCCTGCATT TGGCTTGCCT 
GAGTACTCCT CACCTGCTCC TCAAGGGGAT TACCAGCCTA AGTCTGTACC TGCTGACTTC 
TATGGGGCAG GATCCAGACA ACAGCAGCAG CAGCAGCAGC AGCAAGCCCC CACCACAACT 
TTTATTCACC CACTAACTGG AAAAGTACTG GACCCTTCAT CCCCTCTAGG CTTGGCTTTG 
GCTGCACGGG AACGTGCCTT GAAGGATGAT GGACGTATAC GTAGGGAAAG GACCACTGAA 
CATCATTTTA CCCGTCAGTT GTCAAGCATT GGGGCCTTTT CCTCATCCAC TTCACAGCCA 
ACATCACACA TTTCCTACCC AATGGCCTCG TCTTCCTCTG CAACCTCCTC TGCTGCTCAA 
TCCACAAACA CCTCTTCATA TCTTGTTACC TCCTCATCAT TAGCTGCCAC CACCACATCC 
ACTCGGCCTC CATCTCCTAG GATCCTGCGT GGAGCAGGGA GTAGCTGGAG TGAAGAAGCT 
GGGGTTGGAG AGCGTGCGGA TAGGGAAGCA GCTGGCACCT CTGCTCCCAG AGAAGGCCTA 
AGGGTTCGTT TCTCCGAGGA TAAAACTGTC CACACACACC ACTATCAATC TCAGCAATAT 
CAGCCCACCT TTAAAGAGCG TGAAAGAGAA CGTCAAAGAG AGAGGTCAAG AGAAAGAGAG 
AGGGAAAGAG AGAGGGAAAG AGAAATAGAA AAAGCTCAAA CCCAGTATCA GCAACAGTCA 
TCACAACCAA TGACCCAGCA GCCTCGACGC CCCTCATTCT TGAGGATGGA GAGTGAAGCT 
GGAGCGTACA TTCTTTCATT AACGCCTCCA TCTCCTCCTC CAACAGCAGC CCCTATGCGT 
CCTACAGCAG GAGCCACAGG TGAAAGTGGA AGTGGTACAA GCCTGATGGT TCTGCCTCCC 
CCAGCTCCCT CAGTAGATGC AGATGATGAG TTTGTGTTTG CTGATCCTCT TCCTCCACCT 
CTAGAGTTTG CCAATAGTTT TGATCGTGGT TTGGGTTACT CTGCGATGAT TTCTCACAAT 
GTTGCAGCCC GTCAGCAGCG TCATCCTCCA CCACCCCCTC CCCCACCACC CCCGCCCACT 
TCGAAAGATT CCTTTATGAC AGGCTTTCCA GCTCACACAC CCCTACCGTC ACCTCAGGCA 
GGAGATTCCA CAACTTCCAG TCTTACCTCC TATGACAGTG AGGTTGCAAA CCTAACACAA 
TCTGCCCCAT CGCCCTCCCC ATCTTCTCCC AACCCACTAC CACCTCACTC CCCCTCCACC 
CTTCAGACAT CTGGACCACC TCCACCTCCC TCTGTATCAC CTCCTCCACC ACCCAACAGC 
TACCATAGAC CTTTTGGCTT ATCTCAGTCA CAACATTTAT ACAACAGTAC ACACACAACT 
GGACGCTCCT CCTCTCCCAC CCCACCTCTC TCTATTCCAG CTCCACCTGC CTACCGGGGG 
CCACCAACAG CATCATCCAC CCCTGCAACT TCTGTATCAA TCAGAGGTGC CTCAACTGTG 
ACCGCAAGCT GTTCCACAAC TGCTTCTACA ACTGTCACCT CTCCTAGTAC TACTGCTGCT 
GCCACACCAC ACACACGCAT AGCAACTGTC TCCACAGTAG CTACAGGGGG TAGTAGGCGT 
CCCAGTGCAG AGCATCATCC ACCACCTAGT GCAACCAAGA GTGGTGAGTC CCAGGAGACA 
GTGGTAGATT CTGGGATTGA GGAGCTGGAC AGCCGAAGCA GCAGTGATCA TCACTTAGAC 
AGCATTCTTG CACTCAGTGG TAGTGGGGTC AGAGAGAGGT TGGAACGAGC AGGGATGAGT 
AGCATAGGTG GAATTGGAGG GAGAGATGGT GCAACTGAGG TTGACAGGAC AAGTGGGGCA 
GACTTTTTAG AGTCTTACAT GAGCTACCTT GATGGGCAGA CTTTTGAAAT GCACAATGCC 
AAAGCCGCTA GCACTGCATC CACCTACCCA AAAACACAGA GGTACAGAGA AGGCACCCGG 
GCTGGAGATC TTCGACGCCA GACCAGCACA GCACCTCCTG CATACCACCG TCGTAGAGAG 
GAGGAAGAGG AAGAGGATGA GGCCGAGGAG GTAGAGGAGG AGGATTTGAG TGCCCGTGAT 
GGCTATGGGA TGAGGGAAGG GCCAGGGATT GCTCGCTCCT CTGTAATGTA CTTTGACCGG 
CCCCGCACTC CAGAGATAAA ACCACTGTGG GGAGATGGGT CGACTGGAGC ATCACAGACC 
ATGGGGGAGG GAGGGGGCCA GATAGTGGGA ACATATGTGG AGCCACGTAA ACTACATCCT 
CCCATGACTG GTATGAAAGC AAACATCATT AATGAACTAA GCACCAAATT ACAGCAGAGA 
AGTAAAGGAA CAGAGAACTG GGGGGCCCAG AGATCATTGA GCAGACATAG ACACAGGTTC 
TCAGAGGACT CAACAGGTGC ACTGAGTCCT CCCTCTGTCC GCTCTAGCTC CCCATCTCCA 
GTTCAGCTCT CTCAGCCTGC CTTGTCTCCC TCTCCCACTC CTTCCTCCCA GCCACTTTAC 
TCAAACTGGG CTCGATCTCC TTCCCCTCAG CCCCCTGCTG CCTCATCCCA GCCCCCTCCC 
CGATCCCATT CACCTGTTTC CCCTTCATCA TATTCACCAT ACCCCACCTC ACCCAAACAC 
CGGCCTGTTT ATCGCACCAA AGCTCTGGAG TTCCAATTTA TTCCCAGTCG AGAGTCACGA 
AAGTCAGAGT CGCATCATGC CCAGCGCAGA CGAGCACCCA GCCCTTTAAT TTCTCCATCT 
GACCGTCCCA AGCTTGGCCC TCCCCGCCCC TCTTCCCTTC CTATTCTCCC CACTACACCC 
CTCTATAGCA GCCTGTATGA CCTTCGTGGT TCTATTACTC CACCCTCACC TGCCAATCCC 
CTTGGGGACC CCTACTTTTC CCCCTCCCCC CCTCTATTTG CCCCCTCGGG TGCTCCCCCT 
CCTCCAAATT CCTCTCTGGT GGTCTCACGG TCCCTGTCAC CCACTCACTT CCTGTCAGGG 
ACTTCATCCC CACCTCTCCA CCCCATTCCT CCACCCACTT GCCTGAGTTA CCCCCATCTT 
CCACCCCCCT CACCCACAAA GCCCTTTGCC TCAAAACCTC TCCCATACTG GACCAAGTAT 
GATGTTGCAG ATTGGCTGGG CTACCTGAAC CTGGGTGAAC ACAGGGAGCG ATTCTTAGAC 
AATGAAATTG ATGGCACGCA CCTGCCCTCT CTTACCAAGG ATGACTACCT GGACCTGGGT 
GTGACCCGAG TTGGCCATCG CATGAACATA GAGAGGGCGC TCAGGAGAGT TATGGACAGA 
ACCATCAGTA AAGATCTCAT CCCCTCCATC TCCATATCTT TCATCTGTTC ATCTTCTAAG 
AGTGTGGCAT CTTCATTCAT CATACTGCTC ACTTTACCTT ACTCATACTG GACAAAAAGA 
TTGAGGAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297766.1
CDS	825..8276
Translation	MTMPLSPLSSDEEQQRMLGNSQHLYPGAEEEEEEEEEMDDDGMDEEGEEENGELEMDDEEEVDDEDDIRRGPGHLRGGREEGHRQKGTMAGRLPGDRPIRPGNPGHAANPYSSNPGPTHQTSTNQQQQQPQRKLRERSSSNAPDDTHVALMVFRIGIPDIKQTKCLRFNPDATVWKAKQQVMCSLTESLRDVLNYGLFQPATDGHDAKFLEEERPLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKASLKKFMDYIQTSAVEKVAKLIDKGLDPNYHDPDTGETPLTLAVQTEPGGSEIIRVLVMGGAHIDFRARDGLTPLHKAVRAHAHTALLTLLSLGASSDYKDSRGLTPLYHTVLIGGDTSCCETLLYHRARLGLRDENGWDETHQACQNGNSQHLEHLLFYGADSSSQNASGNTALHICALYNKESCARILLYRGAKKDTKNNSGQNSFQVAVMSGHFELGEIIKNHRESDVVPFLESPKYAPQRRESSRTLGLPHPHPFLRANSDNSMNVPDWIAFPNAAGSSIVSVQGLKHGGTLRSSSSPRGARTRSPSRGRTGDRDDRSRQTRGRQGAGSISSQGTASGQRRRLYSAVPGRVFVATRSHSAQGDREISFNKGDKVKGVDKRCLLLSVGEGGYWEGTVRGRTGWFPSDCVEEVALRSLESRSESRSERAKRLFRHYTVGSYDSFDAPSDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMVDRASAPWKKKSEFESSQATEKKRTVYQMALNKLDEILAAAQQTINTNEAPGPRVQGVKRERGRGFYNEPNFDQTGMGMTSSGAGLGYDRGQFVSGHGPPHGVPEEEKQFLHPPAMKFARSLSVPGPDEIPPPPTTAPPEPPFSSAPPLGFRGKTSQQPSVSMSQSTSSAAHYQLYSQSVHVTHGPRDRSPGPPTGSGTGDLAYSHAQAHPSVPSRTASRKVIGYTGDLAGAGGVAGAKAAALRRGYSNAVPPTSVATVIPQQQQTTQNQPSRMDRSGMGVGAGGVPKGGARRGKGPLVKQSKVEDLRQGQDTLEAKVSVEKSSIPIPTIIVKAPSTSSSGRSSQGSSVEAEPPPPGESDSSKPEPVVPPPAPTVPPPAPPSIPAPPPPSLPSLRTQESLDFTSQFGAAIVGAARRDRERFHEARRKSASFFLSAEEELSGAGGAGGRTQTSQQQLSSQPSPRLRPSKSIDEGMFSGDTFIHHTRSMPPAFGLPEYSSPAPQGDYQPKSVPADFYGAGSRQQQQQQQQQAPTTTFIHPLTGKVLDPSSPLGLALAARERALKDDGRIRRERTTEHHFTRQLSSIGAFSSSTSQPTSHISYPMASSSSATSSAAQSTNTSSYLVTSSSLAATTTSTRPPSPRILRGAGSSWSEEAGVGERADREAAGTSAPREGLRVRFSEDKTVHTHHYQSQQYQPTFKERERERQRERSREREREREREREIEKAQTQYQQQSSQPMTQQPRRPSFLRMESEAGAYILSLTPPSPPPTAAPMRPTAGATGESGSGTSLMVLPPPAPSVDADDEFVFADPLPPPLEFANSFDRGLGYSAMISHNVAARQQRHPPPPPPPPPPPTSKDSFMTGFPAHTPLPSPQAGDSTTSSLTSYDSEVANLTQSAPSPSPSSPNPLPPHSPSTLQTSGPPPPPSVSPPPPPNSYHRPFGLSQSQHLYNSTHTTGRSSSPTPPLSIPAPPAYRGPPTASSTPATSVSIRGASTVTASCSTTASTTVTSPSTTAAATPHTRIATVSTVATGGSRRPSAEHHPPPSATKSGESQETVVDSGIEELDSRSSSDHHLDSILALSGSGVRERLERAGMSSIGGIGGRDGATEVDRTSGADFLESYMSYLDGQTFEMHNAKAASTASTYPKTQRYREGTRAGDLRRQTSTAPPAYHRRREEEEEEDEAEEVEEEDLSARDGYGMREGPGIARSSVMYFDRPRTPEIKPLWGDGSTGASQTMGEGGGQIVGTYVEPRKLHPPMTGMKANIINELSTKLQQRSKGTENWGAQRSLSRHRHRFSEDSTGALSPPSVRSSSPSPVQLSQPALSPSPTPSSQPLYSNWARSPSPQPPAASSQPPPRSHSPVSPSSYSPYPTSPKHRPVYRTKALEFQFIPSRESRKSESHHAQRRRAPSPLISPSDRPKLGPPRPSSLPILPTTPLYSSLYDLRGSITPPSPANPLGDPYFSPSPPLFAPSGAPPPPNSSLVVSRSLSPTHFLSGTSSPPLHPIPPPTCLSYPHLPPPSPTKPFASKPLPYWTKYDVADWLGYLNLGEHRERFLDNEIDGTHLPSLTKDDYLDLGVTRVGHRMNIERALRRVMDRTISKDLIPSISISFICSSSKSVASSFIILLTLPYSYWTKRLRN

Target ORF information:

RefSeq Version	XM_009299491.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299491.2

ORF Insert Sequence:

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGGTGTGGA TAAACGTTGT 
CTGTTGCTGA GTGTGGGTGA AGGAGGCTAT TGGGAAGGGA CGGTTCGAGG ACGCACAGGC 
TGGTTCCCCT CAGACTGCGT GGAGGAAGTT GCTCTCCGTA GCCTGGAATC TCGTTCAGAG 
AGCCGCAGTG AGAGAGCCAA GAGACTCTTC CGGCATTATA CTGTTGGATC CTACGACAGC 
TTCGATGCGC CCAGTGACTA CATAATCAAG GAGAAGACAG TCCTCCTGCA GAAAAAAGAC 
AATGAAGGTT TTGGCTTTGT GCTGAGAGGA GCCAAGGCTC AGACCCCTAT AGAAGAGTTC 
ACTCCCACCC CAGCCTTTCC AGCACTGCAG TACCTTGAGT CTGTCGACGA GGGAGGCGTG 
GCCTGGAGAG CTGGTCTGCG AATGGGAGAC TTCCTTATAG AGGTGAATGG TCAGAATGTG 
GTTAAGGTGG GACACAGGCA GGTGGTCAAT ATGATCAGGC AAGGCGGTAA CAGTCTCATG 
GTCAAGGTTG TCATGGTTAC TCGAAATCCT GATATGGAGG AAGGGGTCAG AAAGAAAATC 
CCACAGCAGA GTAAAAGACT GAGCACACCG GCTATCGCTC TGCGCTCCAA ATCCATGACA 
TCAGAGCTGG AAGAGATGGT GGACAGAGCC TCTGCCCCAT GGAAAAAAAA ATCAGAGTTC 
GAATCTTCAC AAGCTACAGA GAAGAAGAGG ACAGTTTATC AGATGGCTTT GAATAAACTG 
GATGAGATTT TAGCAGCAGC ACAGCAGACC ATTAATACAA ATGAGGCTCC AGGGCCCCGG 
GTTCAGGGGG TAAAAAGAGA ACGGGGCAGA GGCTTTTATA ATGAGCCAAA TTTCGACCAA 
ACTGGAATGG GGATGACATC ATCTGGGGCA GGGTTAGGCT ACGACCGTGG TCAATTTGTG 
TCTGGCCATG GCCCCCCGCA CGGGGTTCCT GAGGAGGAGA AGCAGTTTCT TCATCCTCCA 
GCCATGAAGT TTGCCCGTAG TCTTTCAGTA CCTGGCCCAG ATGAAATCCC TCCTCCACCT 
ACTACAGCAC CCCCTGAGCC CCCTTTTTCC TCAGCTCCTC CGCTTGGCTT TAGGGGCAAG 
ACATCTCAAC AGCCGTCTGT TTCTATGTCT CAGTCTACTT CCTCTGCTGC ACATTATCAA 
CTCTACTCAC AGTCAGTACA TGTTACTCAC GGACCAAGGG ACAGGTCCCC TGGACCACCT 
ACTGGGTCTG GAACTGGGGA CCTTGCTTAT TCCCATGCCC AAGCCCACCC CTCTGTACCA 
AGTAGGACTG CCTCCAGGAA GGTGATTGGG TACACAGGAG ATCTTGCAGG GGCTGGAGGA 
GTGGCTGGGG CCAAAGCTGC AGCATTGAGA AGGGGTTACA GCAATGCAGT CCCACCCACC 
AGTGTCGCTA CTGTGATTCC ACAACAGCAG CAGACTACTC AGAACCAACC TTCCAGAATG 
GACCGGAGTG GAATGGGGGT AGGGGCTGGT GGTGTACCAA AAGGCGGTGC TCGTAGAGGA 
AAGGGGCCCT TGGTGAAACA GTCAAAGGTG GAAGACCTGC GTCAAGGTCA AGACACTCTA 
GAAGCAAAGG TGTCAGTGGA GAAGAGCTCT ATTCCAATCC CAACTATCAT TGTTAAAGCC 
CCTTCCACCA GCAGTAGTGG ACGCAGTAGC CAAGGCAGCA GTGTGGAGGC TGAACCACCG 
CCCCCAGGAG AAAGTGACAG TTCTAAACCA GAACCTGTTG TTCCCCCACC TGCACCAACC 
GTGCCACCAC CAGCCCCTCC ATCTATACCT GCTCCTCCAC CCCCATCACT GCCATCACTT 
CGTACTCAAG AGAGTCTGGA CTTCACCAGC CAGTTTGGTG CTGCCATTGT AGGGGCAGCT 
CGCAGGGACA GAGAACGATT TCATGAAGCA CGACGCAAGA GTGCCTCCTT TTTTCTTTCT 
GCTGAGGAAG AGTTGAGTGG AGCTGGAGGA GCAGGAGGGC GGACTCAGAC TTCTCAACAG 
CAGCTGAGTT CCCAGCCTTC ACCACGCCTG CGACCATCCA AATCTATAGA TGAAGGCATG 
TTCTCAGGTG ATACTTTTAT CCATCACACA CGCAGTATGC CTCCTGCATT TGGCTTGCCT 
GAGTACTCCT CACCTGCTCC TCAAGGGGAT TACCAGCCTA AGTCTGTACC TGCTGACTTC 
TATGGGGCAG GATCCAGACA ACAGCAGCAG CAGCAGCAGC AGCAAGCCCC CACCACAACT 
TTTATTCACC CACTAACTGG AAAAGTACTG GACCCTTCAT CCCCTCTAGG CTTGGCTTTG 
GCTGCACGGG AACGTGCCTT GAAGGATGAT GGACGTATAC GTAGGGAAAG GACCACTGAA 
CATCATTTTA CCCGTCAGTT GTCAAGCATT GGGGCCTTTT CCTCATCCAC TTCACAGCCA 
ACATCACACA TTTCCTACCC AATGGCCTCG TCTTCCTCTG CAACCTCCTC TGCTGCTCAA 
TCCACAAACA CCTCTTCATA TCTTGTTACC TCCTCATCAT TAGCTGCCAC CACCACATCC 
ACTCGGCCTC CATCTCCTAG GATCCTGCGT GGAGCAGGGA GTAGCTGGAG TGAAGAAGCT 
GGGGTTGGAG AGCGTGCGGA TAGGGAAGCA GCTGGCACCT CTGCTCCCAG AGAAGGCCTA 
AGGGTTCGTT TCTCCGAGGA TAAAACTGTC CACACACACC ACTATCAATC TCAGCAATAT 
CAGCCCACCT TTAAAGAGCG TGAAAGAGAA CGTCAAAGAG AGAGGTCAAG AGAAAGAGAG 
AGGGAAAGAG AGAGGGAAAG AGAAATAGAA AAAGCTCAAA CCCAGTATCA GCAACAGTCA 
TCACAACCAA TGACCCAGCA GCCTCGACGC CCCTCATTCT TGAGGATGGA GAGTGAAGCT 
GGAGCGTACA TTCTTTCATT AACGCCTCCA TCTCCTCCTC CAACAGCAGC CCCTATGCGT 
CCTACAGCAG GAGCCACAGG TGAAAGTGGA AGTGGTACAA GCCTGATGGT TCTGCCTCCC 
CCAGCTCCCT CAGTAGATGC AGATGATGAG TTTGTGTTTG CTGATCCTCT TCCTCCACCT 
CTAGAGTTTG CCAATAGTTT TGATCGTGGT TTGGGTTACT CTGCGATGAT TTCTCACAAT 
GTTGCAGCCC GTCAGCAGCG TCATCCTCCA CCACCCCCTC CCCCACCACC CCCGCCCACT 
TCGAAAGATT CCTTTATGAC AGGCTTTCCA GCTCACACAC CCCTACCGTC ACCTCAGGCA 
GGAGATTCCA CAACTTCCAG TCTTACCTCC TATGACAGTG AGGTTGCAAA CCTAACACAA 
TCTGCCCCAT CGCCCTCCCC ATCTTCTCCC AACCCACTAC CACCTCACTC CCCCTCCACC 
CTTCAGACAT CTGGACCACC TCCACCTCCC TCTGTATCAC CTCCTCCACC ACCCAACAGC 
TACCATAGAC CTTTTGGCTT ATCTCAGTCA CAACATTTAT ACAACAGTAC ACACACAACT 
GGACGCTCCT CCTCTCCCAC CCCACCTCTC TCTATTCCAG CTCCACCTGC CTACCGGGGG 
CCACCAACAG CATCATCCAC CCCTGCAACT TCTGTATCAA TCAGAGGTGC CTCAACTGTG 
ACCGCAAGCT GTTCCACAAC TGCTTCTACA ACTGTCACCT CTCCTAGTAC TACTGCTGCT 
GCCACACCAC ACACACGCAT AGCAACTGTC TCCACAGTAG CTACAGGGGG TAGTAGGCGT 
CCCAGTGCAG AGCATCATCC ACCACCTAGT GCAACCAAGA GTGGTGAGTC CCAGGAGACA 
GTGGTAGATT CTGGGATTGA GGAGCTGGAC AGCCGAAGCA GCAGTGATCA TCACTTAGAC 
AGCATTCTTG CACTCAGTGG TAGTGGGGTC AGAGAGAGGT TGGAACGAGC AGGGATGAGT 
AGCATAGGTG GAATTGGAGG GAGAGATGGT GCAACTGAGG TTGACAGGAC AAGTGGGGCA 
GACTTTTTAG AGTCTTACAT GAGCTACCTT GATGGGCAGA CTTTTGAAAT GCACAATGCC 
AAAGCCGCTA GCACTGCATC CACCTACCCA AAAACACAGA GGTACAGAGA AGGCACCCGG 
GCTGGAGATC TTCGACGCCA GACCAGCACA GCACCTCCTG CATACCACCG TCGTAGAGAG 
GAGGAAGAGG AAGAGGATGA GGCCGAGGAG GTAGAGGAGG AGGATTTGAG TGCCCGTGAT 
GGCTATGGGA TGAGGGAAGG GCCAGGGATT GCTCGCTCCT CTGTAATGTA CTTTGACCGG 
CCCCGCACTC CAGAGATAAA ACCACTGTGG GGAGATGGGT CGACTGGAGC ATCACAGACC 
ATGGGGGAGG GAGGGGGCCA GATAGTGGGA ACATATGTGG AGCCACGTAA ACTACATCCT 
CCCATGACTG GTATGAAAGC AAACATCATT AATGAACTAA GCACCAAATT ACAGCAGAGA 
AGTAAAGGAA CAGAGAACTG GGGGGCCCAG AGATCATTGA GCAGACATAG ACACAGGTTC 
TCAGAGGACT CAACAGGTGC ACTGAGTCCT CCCTCTGTCC GCTCTAGCTC CCCATCTCCA 
GTTCAGCTCT CTCAGCCTGC CTTGTCTCCC TCTCCCACTC CTTCCTCCCA GCCACTTTAC 
TCAAACTGGG CTCGATCTCC TTCCCCTCAG CCCCCTGCTG CCTCATCCCA GCCCCCTCCC 
CGATCCCATT CACCTGTTTC CCCTTCATCA TATTCACCAT ACCCCACCTC ACCCAAACAC 
CGGCCTGTTT ATCGCACCAA AGCTCTGGAG TTCCAATTTA TTCCCAGTCG AGAGTCACGA 
AAGTCAGAGT CGCATCATGC CCAGCGCAGA CGAGCACCCA GCCCTTTAAT TTCTCCATCT 
GACCGTCCCA AGCTTGGCCC TCCCCGCCCC TCTTCCCTTC CTATTCTCCC CACTACACCC 
CTCTATAGCA GCCTGTATGA CCTTCGTGGT TCTATTACTC CACCCTCACC TGCCAATCCC 
CTTGGGGACC CCTACTTTTC CCCCTCCCCC CCTCTATTTG CCCCCTCGGG TGCTCCCCCT 
CCTCCAAATT CCTCTCTGGT GGTCTCACGG TCCCTGTCAC CCACTCACTT CCTGTCAGGG 
ACTTCATCCC CACCTCTCCA CCCCATTCCT CCACCCACTT GCCTGAGTTA CCCCCATCTT 
CCACCCCCCT CACCCACAAA GCCCTTTGCC TCAAAACCTC TCCCATACTG GACCAAGTAT 
GATGTTGCAG ATTGGCTGGG CTACCTGAAC CTGGGTGAAC ACAGGGAGCG ATTCTTAGAC 
AATGAAATTG ATGGCACGCA CCTGCCCTCT CTTACCAAGG ATGACTACCT GGACCTGGGT 
GTGACCCGAG TTGGCCATCG CATGAACATA GAGAGGGCGC TCAGGAGAGT TATGGACAGA 
ACCATCAGTA AAGATCTCAT CCCCTCCATC TCCATATCTT TCATCTGTTC ATCTTCTAAG 
AGTGTGGCAT CTTCATTCAT CATACTGCTC ACTTTACCTT ACTCATACTG GACAAAAAGA 
TTGAGGAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_009299492.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297767.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X7, mRNA.

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CloneID	ODa45595
Clone ID Related Accession (Same CDS sequence)	XM_009299492.2
Accession Version	XM_009299492.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299492.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGGTGTGGA TAAACGTTGT 
CTGTTGCTGA GTGTGGGTGA AGGAGGCTAT TGGGAAGGGA CGGTTCGAGG ACGCACAGGC 
TGGTTCCCCT CAGACTGCGT GGAGGAAGTT GCTCTCCGTA GCCTGGAATC TCGTTCAGAG 
AGCCGCAGTG AGAGAGCCAA GAGACTCTTC CGGCATTATA CTGTTGGATC CTACGACAGC 
TTCGATGCGC CCAGTGACTA CATAATCAAG GAGAAGACAG TCCTCCTGCA GAAAAAAGAC 
AATGAAGGTT TTGGCTTTGT GCTGAGAGGA GCCAAGGCTC AGACCCCTAT AGAAGAGTTC 
ACTCCCACCC CAGCCTTTCC AGCACTGCAG TACCTTGAGT CTGTCGACGA GGGAGGCGTG 
GCCTGGAGAG CTGGTCTGCG AATGGGAGAC TTCCTTATAG AGGTGAATGG TCAGAATGTG 
GTTAAGGTGG GACACAGGCA GGTGGTCAAT ATGATCAGGC AAGGCGGTAA CAGTCTCATG 
GTCAAGGTTG TCATGGTTAC TCGAAATCCT GATATGGAGG AAGGGGTCAG AAAGAAAATC 
CCACAGCAGA GTAAAAGACT GAGCACACCG GCTATCGCTC TGCGCTCCAA ATCCATGACA 
TCAGAGCTGG AAGAGATGGT GGACAGAGCC TCTGCCCCAT GGAAAAAAAA ATCAGAGTTC 
GAATCTTCAC AAGCTACAGA GAAGAAGAGG ACAGTTTATC AGATGGCTTT GAATAAACTG 
GATGAGATTT TAGCAGCAGC ACAGCAGACC ATTAATACAA ATGAGGCTCC AGGGCCCCGG 
GTTCAGGGGG TAAAAAGAGA ACGGGGCAGA GGCTTTTATA ATGAGCCAAA TTTCGACCAA 
ACTGGAATGG GGATGACATC ATCTGGGGCA GGGTTAGGCT ACGACCGTGG TCAATTTGTG 
TCTGGCCATG GCCCCCCGCA CGGTATGCTG CGACAGAAGT CTATCGGGGT TCCTGAGGAG 
GAGAAGCAGT TTCTTCATCC TCCAGCCATG AAGTTTGCCC GTAGTCTTTC AGTACCTGGC 
CCAGATGAAA TCCCTCCTCC ACCTACTACA GCACCCCCTG AGCCCCCTTT TTCCTCAGCT 
CCTCCGCTTG GCTTTAGGGG CAAGACATCT CAACAGCCGT CTGTTTCTAT GTCTCAGTCT 
ACTTCCTCTG CTGCACATTA TCAACTCTAC TCACAGTCAG TACATGTTAC TCACGGACCA 
AGGGACAGGT CCCCTGGACC ACCTACTGGG TCTGGAACTG GGGACCTTGC TTATTCCCAT 
GCCCAAGCCC ACCCCTCTGT ACCAAGTAGG ACTGCCTCCA GGAAGGTGAT TGGGTACACA 
GGAGATCTTG CAGGGGCTGG AGGAGTGGCT GGGGCCAAAG CTGCAGCATT GAGAAGGGGT 
TACAGCAATG CAGTCCCACC CACCAGTGTC GCTACTGTGA TTCCACAACA GCAGCAGACT 
ACTCAGAACC AACCTTCCAG AATGGACCGG AGTGGAATGG GGGTAGGGGC TGGTGGTGTA 
CCAAAAGGCG GTGCTCGTAG AGGAAAGGGG CCCTTGGTGA AACAGTCAAA GGTGGAAGAC 
CTGCGTCAAG GTCAAGACAC TCTAGAAGCA AAGGTGTCAG TGGAGAAGAG CTCTATTCCA 
ATCCCAACTA TCATTGTTAA AGCCCCTTCC ACCAGCAGTA GTGGACGCAG TAGCCAAGGC 
AGCAGTGTGG AGGCTGAACC ACCGCCCCCA GGAGAAAGTG ACAGTTCTAA ACCAGAACCT 
GTTGTTCCCC CACCTGCACC AACCGTGCCA CCACCAGCCC CTCCATCTAT ACCTGCTCCT 
CCACCCCCAT CACTGCCATC ACTTCGTACT CAAGAGAGTC TGGACTTCAC CAGCCAGTTT 
GGTGCTGCCA TTGTAGGGGC AGCTCGCAGG GACAGAGAAC GATTTCATGA AGCACGACGC 
AAGAGTGCCT CCTTTTTTCT TTCTGCTGAG GAAGAGTTGA GTGGAGCTGG AGGAGCAGGA 
GGGCGGACTC AGACTTCTCA ACAGCAGCTG AGTTCCCAGC CTTCACCACG CCTGCGACCA 
TCCAAATCTA TAGATGAAGG CATGTTCTCA GGTGATACTT TTATCCATCA CACACGCAGT 
ATGCCTCCTG CATTTGGCTT GCCTGAGTAC TCCTCACCTG CTCCTCAAGG GGATTACCAG 
CCTAAGTCTG TACCTGCTGA CTTCTATGGG GCAGGATCCA GACAACAGCA GCAGCAGCAG 
CAGCAGCAAG CCCCCACCAC AACTTTTATT CACCCACTAA CTGGAAAAGT ACTGGACCCT 
TCATCCCCTC TAGGCTTGGC TTTGGCTGCA CGGGAACGTG CCTTGAAGGA TGATGGACGT 
ATACGTAGGG AAAGGACCAC TGAACATCAT TTTACCCGTC AGTTGTCAAG CATTGGGGCC 
TTTTCCTCAT CCACTTCACA GCCAACATCA CACATTTCCT ACCCAATGGC CTCGTCTTCC 
TCTGCAACCT CCTCTGCTGC TCAATCCACA AACACCTCTT CATATCTTGT TACCTCCTCA 
TCATTAGCTG CCACCACCAC ATCCACTCGG CCTCCATCTC CTAGGATCCT GCGTGGAGCA 
GGGAGTAGCT GGAGTGAAGA AGCTGGGGTT GGAGAGCGTG CGGATAGGGA AGCAGCTGGC 
ACCTCTGCTC CCAGAGAAGG CCTAAGGGTT CGTTTCTCCG AGGATAAAAC TGTCCACACA 
CACCACTATC AATCTCAGCA ATATCAGCCC ACCTTTAAAG AGCGTGAAAG AGAACGTCAA 
AGAGAGAGGT CAAGAGAAAG AGAGAGGGAA AGAGAGAGGG AAAGAGAAAT AGAAAAAGCT 
CAAACCCAGT ATCAGCAACA GTCATCACAA CCAATGACCC AGCAGCCTCG ACGCCCCTCA 
TTCTTGAGGA TGGAGAGTGA AGCTGGAGCG TACATTCTTT CATTAACGCC TCCATCTCCT 
CCTCCAACAG CAGCCCCTAT GCGTCCTACA GCAGGAGCCA CAGGTGAAAG TGGAAGTGGT 
ACAAGCCTGA TGGTTCTGCC TCCCCCAGCT CCCTCAGTAG ATGCAGATGA TGAGTTTGTG 
TTTGCTGATC CTCTTCCTCC ACCTCTAGAG TTTGCCAATA GTTTTGATCG TGGTTTGGGT 
TACTCTGCGA TGATTTCTCA CAATGTTGCA GCCCGTCAGC AGCGTCATCC TCCACCACCC 
CCTCCCCCAC CACCCCCGCC CACTTCGAAA GATTCCTTTA TGACAGGCTT TCCAGCTCAC 
ACACCCCTAC CGTCACCTCA GGCAGGAGAT TCCACAACTT CCAGTCTTAC CTCCTATGAC 
AGTGAGGTTG CAAACCTAAC ACAATCTGCC CCATCGCCCT CCCCATCTTC TCCCAACCCA 
CTACCACCTC ACTCCCCCTC CACCCTTCAG ACATCTGGAC CACCTCCACC TCCCTCTGTA 
TCACCTCCTC CACCACCCAA CAGCTACCAT AGACCTTTTG GCTTATCTCA GTCACAACAT 
TTATACAACA GTACACACAC AACTGGACGC TCCTCCTCTC CCACCCCACC TCTCTCTATT 
CCAGCTCCAC CTGCCTACCG GGGGCCACCA ACAGCATCAT CCACCCCTGC AACTTCTGTA 
TCAATCAGAG GTGCCTCAAC TGTGACCGCA AGCTGTTCCA CAACTGCTTC TACAACTGTC 
ACCTCTCCTA GTACTACTGC TGCTGCCACA CCACACACAC GCATAGCAAC TGTCTCCACA 
GTAGCTACAG GGGGTAGTAG GCGTCCCAGT GCAGAGCATC ATCCACCACC TAGTGCAACC 
AAGAGTGGTG AGTCCCAGGA GACAGTGGTA GATTCTGGGA TTGAGGAGCT GGACAGCCGA 
AGCAGCAGTG ATCATCACTT AGACAGCATT CTTGCACTCA GTGGTAGTGG GGTCAGAGAG 
AGGTTGGAAC GAGCAGGGAT GAGTAGCATA GGTGGAATTG GAGGGAGAGA TGGTGCAACT 
GAGGTTGACA GGACAAGTGG GGCAGACTTT TTAGAGTCTT ACATGAGCTA CCTTGATGGG 
CAGACTTTTG AAATGCACAA TGCCAAAGCC GCTAGCACTG CATCCACCTA CCCAAAAACA 
CAGAGGTACA GAGAAGGCAC CCGGGCTGGA GATCTTCGAC GCCAGACCAG CACAGCACCT 
CCTGCATACC ACCGTCGTAG AGAGGAGGAA GAGGAAGAGG ATGAGGCCGA GGAGGTAGAG 
GAGGAGGATT TGAGTGCCCG TGATGGCTAT GGGATGAGGG AAGGGCCAGG GATTGCTCGC 
TCCTCTGTAA TGTACTTTGA CCGGCCCCGC ACTCCAGAGA TAAAACCACT GTGGGGAGAT 
GGGTCGACTG GAGCATCACA GACCATGGGG GAGGGAGGGG GCCAGATAGT GGGAACATAT 
GTGGAGCCAC GTAAACTACA TCCTCCCATG ACTGGTATGA AAGCAAACAT CATTAATGAA 
CTAAGCACCA AATTACAGCA GAGAAGTAAA GGAACAGAGA ACTGGGGGGC CCAGAGATCA 
TTGAGCAGAC ATAGACACAG GTTCTCAGAG GACTCAACAG GTGCACTGAG TCCTCCCTCT 
GTCCGCTCTA GCTCCCCATC TCCAGTTCAG CTCTCTCAGC CTGCCTTGTC TCCCTCTCCC 
ACTCCTTCCT CCCAGCCACT TTACTCAAAC TGGGCTCGAT CTCCTTCCCC TCAGCCCCCT 
GCTGCCTCAT CCCAGCCCCC TCCCCGATCC CATTCACCTG TTTCCCCTTC ATCATATTCA 
CCATACCCCA CCTCACCCAA ACACCGGCCT GTTTATCGCA CCAAAGCTCT GGAGTTCCAA 
TTTATTCCCA GTCGAGAGTC ACGAAAGTCA GAGTCGCATC ATGCCCAGCG CAGACGAGCA 
CCCAGCCCTT TAATTTCTCC ATCTGACCGT CCCAAGCTTG GCCCTCCCCG CCCCTCTTCC 
CTTCCTATTC TCCCCACTAC ACCCCTCTAT AGCAGCCTGT ATGACCTTCG TGGTTCTATT 
ACTCCACCCT CACCTGCCAA TCCCCTTGGG GACCCCTACT TTTCCCCCTC CCCCCCTCTA 
TTTGCCCCCT CGGGTGCTCC CCCTCCTCCA AATTCCTCTC TGGTGGTCTC ACGGTCCCTG 
TCACCCACTC ACTTCCTGTC AGGGACTTCA TCCCCACCTC TCCACCCCAT TCCTCCACCC 
ACTTGCCTGA GTTACCCCCA TCTTCCACCC CCCTCACCCA CAAAGCCCTT TGCCTCAAAA 
CCTCTCCCAT ACTGGACCAA GTATGATGTT GCAGATTGGC TGGGCTACCT GAACCTGGGT 
GAACACAGGG AGCGATTCTT AGACAATGAA ATTGATGGCA CGCACCTGCC CTCTCTTACC 
AAGGATGACT ACCTGGACCT GGGTGTGACC CGAGTTGGCC ATCGCATGAA CATAGAGAGG 
GCGCTCAGGA GAGTTATGGA CAGGCTGTCT TCTAGCACTT TCCCCATTTC TGCTCTCTCT 
CCTCGAAATG GACGGACAAA CAGAAGGAGA GATGAGGCCA ACCGGAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297767.1
CDS	825..8255
Translation	MTMPLSPLSSDEEQQRMLGNSQHLYPGAEEEEEEEEEMDDDGMDEEGEEENGELEMDDEEEVDDEDDIRRGPGHLRGGREEGHRQKGTMAGRLPGDRPIRPGNPGHAANPYSSNPGPTHQTSTNQQQQQPQRKLRERSSSNAPDDTHVALMVFRIGIPDIKQTKCLRFNPDATVWKAKQQVMCSLTESLRDVLNYGLFQPATDGHDAKFLEEERPLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKASLKKFMDYIQTSAVEKVAKLIDKGLDPNYHDPDTGETPLTLAVQTEPGGSEIIRVLVMGGAHIDFRARDGLTPLHKAVRAHAHTALLTLLSLGASSDYKDSRGLTPLYHTVLIGGDTSCCETLLYHRARLGLRDENGWDETHQACQNGNSQHLEHLLFYGADSSSQNASGNTALHICALYNKESCARILLYRGAKKDTKNNSGQNSFQVAVMSGHFELGEIIKNHRESDVVPFLESPKYAPQRRESSRTLGLPHPHPFLRANSDNSMNVPDWIAFPNAAGSSIVSVQGLKHGGTLRSSSSPRGARTRSPSRGRTGDRDDRSRQTRGRQGAGSISSQGTASGQRRRLYSAVPGRVFVATRSHSAQGDREISFNKGDKVKGVDKRCLLLSVGEGGYWEGTVRGRTGWFPSDCVEEVALRSLESRSESRSERAKRLFRHYTVGSYDSFDAPSDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMVDRASAPWKKKSEFESSQATEKKRTVYQMALNKLDEILAAAQQTINTNEAPGPRVQGVKRERGRGFYNEPNFDQTGMGMTSSGAGLGYDRGQFVSGHGPPHGMLRQKSIGVPEEEKQFLHPPAMKFARSLSVPGPDEIPPPPTTAPPEPPFSSAPPLGFRGKTSQQPSVSMSQSTSSAAHYQLYSQSVHVTHGPRDRSPGPPTGSGTGDLAYSHAQAHPSVPSRTASRKVIGYTGDLAGAGGVAGAKAAALRRGYSNAVPPTSVATVIPQQQQTTQNQPSRMDRSGMGVGAGGVPKGGARRGKGPLVKQSKVEDLRQGQDTLEAKVSVEKSSIPIPTIIVKAPSTSSSGRSSQGSSVEAEPPPPGESDSSKPEPVVPPPAPTVPPPAPPSIPAPPPPSLPSLRTQESLDFTSQFGAAIVGAARRDRERFHEARRKSASFFLSAEEELSGAGGAGGRTQTSQQQLSSQPSPRLRPSKSIDEGMFSGDTFIHHTRSMPPAFGLPEYSSPAPQGDYQPKSVPADFYGAGSRQQQQQQQQQAPTTTFIHPLTGKVLDPSSPLGLALAARERALKDDGRIRRERTTEHHFTRQLSSIGAFSSSTSQPTSHISYPMASSSSATSSAAQSTNTSSYLVTSSSLAATTTSTRPPSPRILRGAGSSWSEEAGVGERADREAAGTSAPREGLRVRFSEDKTVHTHHYQSQQYQPTFKERERERQRERSREREREREREREIEKAQTQYQQQSSQPMTQQPRRPSFLRMESEAGAYILSLTPPSPPPTAAPMRPTAGATGESGSGTSLMVLPPPAPSVDADDEFVFADPLPPPLEFANSFDRGLGYSAMISHNVAARQQRHPPPPPPPPPPPTSKDSFMTGFPAHTPLPSPQAGDSTTSSLTSYDSEVANLTQSAPSPSPSSPNPLPPHSPSTLQTSGPPPPPSVSPPPPPNSYHRPFGLSQSQHLYNSTHTTGRSSSPTPPLSIPAPPAYRGPPTASSTPATSVSIRGASTVTASCSTTASTTVTSPSTTAAATPHTRIATVSTVATGGSRRPSAEHHPPPSATKSGESQETVVDSGIEELDSRSSSDHHLDSILALSGSGVRERLERAGMSSIGGIGGRDGATEVDRTSGADFLESYMSYLDGQTFEMHNAKAASTASTYPKTQRYREGTRAGDLRRQTSTAPPAYHRRREEEEEEDEAEEVEEEDLSARDGYGMREGPGIARSSVMYFDRPRTPEIKPLWGDGSTGASQTMGEGGGQIVGTYVEPRKLHPPMTGMKANIINELSTKLQQRSKGTENWGAQRSLSRHRHRFSEDSTGALSPPSVRSSSPSPVQLSQPALSPSPTPSSQPLYSNWARSPSPQPPAASSQPPPRSHSPVSPSSYSPYPTSPKHRPVYRTKALEFQFIPSRESRKSESHHAQRRRAPSPLISPSDRPKLGPPRPSSLPILPTTPLYSSLYDLRGSITPPSPANPLGDPYFSPSPPLFAPSGAPPPPNSSLVVSRSLSPTHFLSGTSSPPLHPIPPPTCLSYPHLPPPSPTKPFASKPLPYWTKYDVADWLGYLNLGEHRERFLDNEIDGTHLPSLTKDDYLDLGVTRVGHRMNIERALRRVMDRLSSSTFPISALSPRNGRTNRRRDEANRS

Target ORF information:

RefSeq Version	XM_009299492.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299492.2

ORF Insert Sequence:

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGGTGTGGA TAAACGTTGT 
CTGTTGCTGA GTGTGGGTGA AGGAGGCTAT TGGGAAGGGA CGGTTCGAGG ACGCACAGGC 
TGGTTCCCCT CAGACTGCGT GGAGGAAGTT GCTCTCCGTA GCCTGGAATC TCGTTCAGAG 
AGCCGCAGTG AGAGAGCCAA GAGACTCTTC CGGCATTATA CTGTTGGATC CTACGACAGC 
TTCGATGCGC CCAGTGACTA CATAATCAAG GAGAAGACAG TCCTCCTGCA GAAAAAAGAC 
AATGAAGGTT TTGGCTTTGT GCTGAGAGGA GCCAAGGCTC AGACCCCTAT AGAAGAGTTC 
ACTCCCACCC CAGCCTTTCC AGCACTGCAG TACCTTGAGT CTGTCGACGA GGGAGGCGTG 
GCCTGGAGAG CTGGTCTGCG AATGGGAGAC TTCCTTATAG AGGTGAATGG TCAGAATGTG 
GTTAAGGTGG GACACAGGCA GGTGGTCAAT ATGATCAGGC AAGGCGGTAA CAGTCTCATG 
GTCAAGGTTG TCATGGTTAC TCGAAATCCT GATATGGAGG AAGGGGTCAG AAAGAAAATC 
CCACAGCAGA GTAAAAGACT GAGCACACCG GCTATCGCTC TGCGCTCCAA ATCCATGACA 
TCAGAGCTGG AAGAGATGGT GGACAGAGCC TCTGCCCCAT GGAAAAAAAA ATCAGAGTTC 
GAATCTTCAC AAGCTACAGA GAAGAAGAGG ACAGTTTATC AGATGGCTTT GAATAAACTG 
GATGAGATTT TAGCAGCAGC ACAGCAGACC ATTAATACAA ATGAGGCTCC AGGGCCCCGG 
GTTCAGGGGG TAAAAAGAGA ACGGGGCAGA GGCTTTTATA ATGAGCCAAA TTTCGACCAA 
ACTGGAATGG GGATGACATC ATCTGGGGCA GGGTTAGGCT ACGACCGTGG TCAATTTGTG 
TCTGGCCATG GCCCCCCGCA CGGTATGCTG CGACAGAAGT CTATCGGGGT TCCTGAGGAG 
GAGAAGCAGT TTCTTCATCC TCCAGCCATG AAGTTTGCCC GTAGTCTTTC AGTACCTGGC 
CCAGATGAAA TCCCTCCTCC ACCTACTACA GCACCCCCTG AGCCCCCTTT TTCCTCAGCT 
CCTCCGCTTG GCTTTAGGGG CAAGACATCT CAACAGCCGT CTGTTTCTAT GTCTCAGTCT 
ACTTCCTCTG CTGCACATTA TCAACTCTAC TCACAGTCAG TACATGTTAC TCACGGACCA 
AGGGACAGGT CCCCTGGACC ACCTACTGGG TCTGGAACTG GGGACCTTGC TTATTCCCAT 
GCCCAAGCCC ACCCCTCTGT ACCAAGTAGG ACTGCCTCCA GGAAGGTGAT TGGGTACACA 
GGAGATCTTG CAGGGGCTGG AGGAGTGGCT GGGGCCAAAG CTGCAGCATT GAGAAGGGGT 
TACAGCAATG CAGTCCCACC CACCAGTGTC GCTACTGTGA TTCCACAACA GCAGCAGACT 
ACTCAGAACC AACCTTCCAG AATGGACCGG AGTGGAATGG GGGTAGGGGC TGGTGGTGTA 
CCAAAAGGCG GTGCTCGTAG AGGAAAGGGG CCCTTGGTGA AACAGTCAAA GGTGGAAGAC 
CTGCGTCAAG GTCAAGACAC TCTAGAAGCA AAGGTGTCAG TGGAGAAGAG CTCTATTCCA 
ATCCCAACTA TCATTGTTAA AGCCCCTTCC ACCAGCAGTA GTGGACGCAG TAGCCAAGGC 
AGCAGTGTGG AGGCTGAACC ACCGCCCCCA GGAGAAAGTG ACAGTTCTAA ACCAGAACCT 
GTTGTTCCCC CACCTGCACC AACCGTGCCA CCACCAGCCC CTCCATCTAT ACCTGCTCCT 
CCACCCCCAT CACTGCCATC ACTTCGTACT CAAGAGAGTC TGGACTTCAC CAGCCAGTTT 
GGTGCTGCCA TTGTAGGGGC AGCTCGCAGG GACAGAGAAC GATTTCATGA AGCACGACGC 
AAGAGTGCCT CCTTTTTTCT TTCTGCTGAG GAAGAGTTGA GTGGAGCTGG AGGAGCAGGA 
GGGCGGACTC AGACTTCTCA ACAGCAGCTG AGTTCCCAGC CTTCACCACG CCTGCGACCA 
TCCAAATCTA TAGATGAAGG CATGTTCTCA GGTGATACTT TTATCCATCA CACACGCAGT 
ATGCCTCCTG CATTTGGCTT GCCTGAGTAC TCCTCACCTG CTCCTCAAGG GGATTACCAG 
CCTAAGTCTG TACCTGCTGA CTTCTATGGG GCAGGATCCA GACAACAGCA GCAGCAGCAG 
CAGCAGCAAG CCCCCACCAC AACTTTTATT CACCCACTAA CTGGAAAAGT ACTGGACCCT 
TCATCCCCTC TAGGCTTGGC TTTGGCTGCA CGGGAACGTG CCTTGAAGGA TGATGGACGT 
ATACGTAGGG AAAGGACCAC TGAACATCAT TTTACCCGTC AGTTGTCAAG CATTGGGGCC 
TTTTCCTCAT CCACTTCACA GCCAACATCA CACATTTCCT ACCCAATGGC CTCGTCTTCC 
TCTGCAACCT CCTCTGCTGC TCAATCCACA AACACCTCTT CATATCTTGT TACCTCCTCA 
TCATTAGCTG CCACCACCAC ATCCACTCGG CCTCCATCTC CTAGGATCCT GCGTGGAGCA 
GGGAGTAGCT GGAGTGAAGA AGCTGGGGTT GGAGAGCGTG CGGATAGGGA AGCAGCTGGC 
ACCTCTGCTC CCAGAGAAGG CCTAAGGGTT CGTTTCTCCG AGGATAAAAC TGTCCACACA 
CACCACTATC AATCTCAGCA ATATCAGCCC ACCTTTAAAG AGCGTGAAAG AGAACGTCAA 
AGAGAGAGGT CAAGAGAAAG AGAGAGGGAA AGAGAGAGGG AAAGAGAAAT AGAAAAAGCT 
CAAACCCAGT ATCAGCAACA GTCATCACAA CCAATGACCC AGCAGCCTCG ACGCCCCTCA 
TTCTTGAGGA TGGAGAGTGA AGCTGGAGCG TACATTCTTT CATTAACGCC TCCATCTCCT 
CCTCCAACAG CAGCCCCTAT GCGTCCTACA GCAGGAGCCA CAGGTGAAAG TGGAAGTGGT 
ACAAGCCTGA TGGTTCTGCC TCCCCCAGCT CCCTCAGTAG ATGCAGATGA TGAGTTTGTG 
TTTGCTGATC CTCTTCCTCC ACCTCTAGAG TTTGCCAATA GTTTTGATCG TGGTTTGGGT 
TACTCTGCGA TGATTTCTCA CAATGTTGCA GCCCGTCAGC AGCGTCATCC TCCACCACCC 
CCTCCCCCAC CACCCCCGCC CACTTCGAAA GATTCCTTTA TGACAGGCTT TCCAGCTCAC 
ACACCCCTAC CGTCACCTCA GGCAGGAGAT TCCACAACTT CCAGTCTTAC CTCCTATGAC 
AGTGAGGTTG CAAACCTAAC ACAATCTGCC CCATCGCCCT CCCCATCTTC TCCCAACCCA 
CTACCACCTC ACTCCCCCTC CACCCTTCAG ACATCTGGAC CACCTCCACC TCCCTCTGTA 
TCACCTCCTC CACCACCCAA CAGCTACCAT AGACCTTTTG GCTTATCTCA GTCACAACAT 
TTATACAACA GTACACACAC AACTGGACGC TCCTCCTCTC CCACCCCACC TCTCTCTATT 
CCAGCTCCAC CTGCCTACCG GGGGCCACCA ACAGCATCAT CCACCCCTGC AACTTCTGTA 
TCAATCAGAG GTGCCTCAAC TGTGACCGCA AGCTGTTCCA CAACTGCTTC TACAACTGTC 
ACCTCTCCTA GTACTACTGC TGCTGCCACA CCACACACAC GCATAGCAAC TGTCTCCACA 
GTAGCTACAG GGGGTAGTAG GCGTCCCAGT GCAGAGCATC ATCCACCACC TAGTGCAACC 
AAGAGTGGTG AGTCCCAGGA GACAGTGGTA GATTCTGGGA TTGAGGAGCT GGACAGCCGA 
AGCAGCAGTG ATCATCACTT AGACAGCATT CTTGCACTCA GTGGTAGTGG GGTCAGAGAG 
AGGTTGGAAC GAGCAGGGAT GAGTAGCATA GGTGGAATTG GAGGGAGAGA TGGTGCAACT 
GAGGTTGACA GGACAAGTGG GGCAGACTTT TTAGAGTCTT ACATGAGCTA CCTTGATGGG 
CAGACTTTTG AAATGCACAA TGCCAAAGCC GCTAGCACTG CATCCACCTA CCCAAAAACA 
CAGAGGTACA GAGAAGGCAC CCGGGCTGGA GATCTTCGAC GCCAGACCAG CACAGCACCT 
CCTGCATACC ACCGTCGTAG AGAGGAGGAA GAGGAAGAGG ATGAGGCCGA GGAGGTAGAG 
GAGGAGGATT TGAGTGCCCG TGATGGCTAT GGGATGAGGG AAGGGCCAGG GATTGCTCGC 
TCCTCTGTAA TGTACTTTGA CCGGCCCCGC ACTCCAGAGA TAAAACCACT GTGGGGAGAT 
GGGTCGACTG GAGCATCACA GACCATGGGG GAGGGAGGGG GCCAGATAGT GGGAACATAT 
GTGGAGCCAC GTAAACTACA TCCTCCCATG ACTGGTATGA AAGCAAACAT CATTAATGAA 
CTAAGCACCA AATTACAGCA GAGAAGTAAA GGAACAGAGA ACTGGGGGGC CCAGAGATCA 
TTGAGCAGAC ATAGACACAG GTTCTCAGAG GACTCAACAG GTGCACTGAG TCCTCCCTCT 
GTCCGCTCTA GCTCCCCATC TCCAGTTCAG CTCTCTCAGC CTGCCTTGTC TCCCTCTCCC 
ACTCCTTCCT CCCAGCCACT TTACTCAAAC TGGGCTCGAT CTCCTTCCCC TCAGCCCCCT 
GCTGCCTCAT CCCAGCCCCC TCCCCGATCC CATTCACCTG TTTCCCCTTC ATCATATTCA 
CCATACCCCA CCTCACCCAA ACACCGGCCT GTTTATCGCA CCAAAGCTCT GGAGTTCCAA 
TTTATTCCCA GTCGAGAGTC ACGAAAGTCA GAGTCGCATC ATGCCCAGCG CAGACGAGCA 
CCCAGCCCTT TAATTTCTCC ATCTGACCGT CCCAAGCTTG GCCCTCCCCG CCCCTCTTCC 
CTTCCTATTC TCCCCACTAC ACCCCTCTAT AGCAGCCTGT ATGACCTTCG TGGTTCTATT 
ACTCCACCCT CACCTGCCAA TCCCCTTGGG GACCCCTACT TTTCCCCCTC CCCCCCTCTA 
TTTGCCCCCT CGGGTGCTCC CCCTCCTCCA AATTCCTCTC TGGTGGTCTC ACGGTCCCTG 
TCACCCACTC ACTTCCTGTC AGGGACTTCA TCCCCACCTC TCCACCCCAT TCCTCCACCC 
ACTTGCCTGA GTTACCCCCA TCTTCCACCC CCCTCACCCA CAAAGCCCTT TGCCTCAAAA 
CCTCTCCCAT ACTGGACCAA GTATGATGTT GCAGATTGGC TGGGCTACCT GAACCTGGGT 
GAACACAGGG AGCGATTCTT AGACAATGAA ATTGATGGCA CGCACCTGCC CTCTCTTACC 
AAGGATGACT ACCTGGACCT GGGTGTGACC CGAGTTGGCC ATCGCATGAA CATAGAGAGG 
GCGCTCAGGA GAGTTATGGA CAGGCTGTCT TCTAGCACTT TCCCCATTTC TGCTCTCTCT 
CCTCGAAATG GACGGACAAA CAGAAGGAGA GATGAGGCCA ACCGGAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_009299493.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297768.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X8, mRNA.

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CloneID	ODa45596
Clone ID Related Accession (Same CDS sequence)	XM_009299493.2
Accession Version	XM_009299493.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299493.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGGTGTGGA TAAACGTTGT 
CTGTTGCTGA GTGTGGGTGA AGGAGGCTAT TGGGAAGGGA CGGTTCGAGG ACGCACAGGC 
TGGTTCCCCT CAGACTGCGT GGAGGAAGTT GCTCTCCGTA GCCTGGAATC TCGTTCAGAG 
AGCCGCAGTG AGAGAGCCAA GAGACTCTTC CGGCATTATA CTGTTGGATC CTACGACAGC 
TTCGATGCGC CCAGTGACTA CATAATCAAG GAGAAGACAG TCCTCCTGCA GAAAAAAGAC 
AATGAAGGTT TTGGCTTTGT GCTGAGAGGA GCCAAGGCTC AGACCCCTAT AGAAGAGTTC 
ACTCCCACCC CAGCCTTTCC AGCACTGCAG TACCTTGAGT CTGTCGACGA GGGAGGCGTG 
GCCTGGAGAG CTGGTCTGCG AATGGGAGAC TTCCTTATAG AGGTGAATGG TCAGAATGTG 
GTTAAGGTGG GACACAGGCA GGTGGTCAAT ATGATCAGGC AAGGCGGTAA CAGTCTCATG 
GTCAAGGTTG TCATGGTTAC TCGAAATCCT GATATGGAGG AAGGGGTCAG AAAGAAAATC 
CCACAGCAGA GTAAAAGACT GAGCACACCG GCTATCGCTC TGCGCTCCAA ATCCATGACA 
TCAGAGCTGG AAGAGATGGT GGACAGAGCC TCTGCCCCAT GGAAAAAAAA ATCAGAGTTC 
GAATCTTCAC AAGCTACAGA GAAGAAGAGG ACAGTTTATC AGATGGCTTT GAATAAACTG 
GATGAGATTT TAGCAGCAGC ACAGCAGACC ATTAATACAA ATGAGGCTCC AGGGCCCCGG 
GTTCAGGGGG TAAAAAGAGA ACGGGGCAGA GGCTTTTATA ATGAGCCAAA TTTCGACCAA 
ACTGGAATGG GGATGACATC ATCTGGGGCA GGGTTAGGCT ACGACCGTGG TCAATTTGTG 
TCTGGCCATG GCCCCCCGCA CGGTATGCTG CGACAGAAGT CTATCGGGGT TCCTGAGGAG 
GAGAAGCAGT TTCTTCATCC TCCAGCCATG AAGTTTGCCC GTAGTCTTTC AGTACCTGGC 
CCAGATGAAA TCCCTCCTCC ACCTACTACA GCACCCCCTG AGCCCCCTTT TTCCTCAGCT 
CCTCCGCTTG GCTTTAGGGG CAAGACATCT CAACAGCCGT CTGTTTCTAT GTCTCAGTCT 
ACTTCCTCTG CTGCACATTA TCAACTCTAC TCACAGTCAG TACATGTTAC TCACGGACCA 
AGGGACAGGT CCCCTGGACC ACCTACTGGG TCTGGAACTG GGGACCTTGC TTATTCCCAT 
GCCCAAGCCC ACCCCTCTGT ACCAAGTAGG ACTGCCTCCA GGAAGGTGAT TGGGTACACA 
GGAGATCTTG CAGGGGCTGG AGGAGTGGCT GGGGCCAAAG CTGCAGCATT GAGAAGGGGT 
TACAGCAATG CAGTCCCACC CACCAGTGTC GCTACTGTGA TTCCACAACA GCAGCAGACT 
ACTCAGAACC AACCTTCCAG AATGGACCGG AGTGGAATGG GGGTAGGGGC TGGTGGTGTA 
CCAAAAGGCG GTGCTCGTAG AGGAAAGGGG CCCTTGGTGA AACAGTCAAA GGTGGAAGAC 
CTGCGTCAAG GTCAAGACAC TCTAGAAGCA AAGGTGTCAG TGGAGAAGAG CTCTATTCCA 
ATCCCAACTA TCATTGTTAA AGCCCCTTCC ACCAGCAGTA GTGGACGCAG TAGCCAAGGC 
AGCAGTGTGG AGGCTGAACC ACCGCCCCCA GGAGAAAGTG ACAGTTCTAA ACCAGAACCT 
GTTGTTCCCC CACCTGCACC AACCGTGCCA CCACCAGCCC CTCCATCTAT ACCTGCTCCT 
CCACCCCCAT CACTGCCATC ACTTCGTACT CAAGAGAGTC TGGACTTCAC CAGCCAGTTT 
GGTGCTGCCA TTGTAGGGGC AGCTCGCAGG GACAGAGAAC GATTTCATGA AGCACGACGC 
AAGAGTGCCT CCTTTTTTCT TTCTGCTGAG GAAGAGTTGA GTGGAGCTGG AGGAGCAGGA 
GGGCGGACTC AGACTTCTCA ACAGCAGCTG AGTTCCCAGC CTTCACCACG CCTGCGACCA 
TCCAAATCTA TAGATGAAGG CATGTTCTCA GGTGATACTT TTATCCATCA CACACGCAGT 
ATGCCTCCTG CATTTGGCTT GCCTGAGTAC TCCTCACCTG CTCCTCAAGG GGATTACCAG 
CCTAAGTCTG TACCTGCTGA CTTCTATGGG GCAGGATCCA GACAACAGCA GCAGCAGCAG 
CAGCAGCAAG CCCCCACCAC AACTTTTATT CACCCACTAA CTGGAAAAGT ACTGGACCCT 
TCATCCCCTC TAGGCTTGGC TTTGGCTGCA CGGGAACGTG CCTTGAAGGA TGATGGACGT 
ATACGTAGGG AAAGGACCAC TGAACATCAT TTTACCCGTC AGTTGTCAAG CATTGGGGCC 
TTTTCCTCAT CCACTTCACA GCCAACATCA CACATTTCCT ACCCAATGGC CTCGTCTTCC 
TCTGCAACCT CCTCTGCTGC TCAATCCACA AACACCTCTT CATATCTTGT TACCTCCTCA 
TCATTAGCTG CCACCACCAC ATCCACTCGG CCTCCATCTC CTAGGATCCT GCGTGGAGCA 
GGGAGTAGCT GGAGTGAAGA AGCTGGGGTT GGAGAGCGTG CGGATAGGGA AGCAGCTGGC 
ACCTCTGCTC CCAGAGAAGG CCTAAGGGTT CGTTTCTCCG AGGATAAAAC TGTCCACACA 
CACCACTATC AATCTCAGCA ATATCAGCCC ACCTTTAAAG AGCGTGAAAG AGAACGTCAA 
AGAGAGAGGT CAAGAGAAAG AGAGAGGGAA AGAGAGAGGG AAAGAGAAAT AGAAAAAGCT 
CAAACCCAGT ATCAGCAACA GTCATCACAA CCAATGACCC AGCAGCCTCG ACGCCCCTCA 
TTCTTGAGGA TGGAGAGTGA AGCTGGAGCG TACATTCTTT CATTAACGCC TCCATCTCCT 
CCTCCAACAG CAGCCCCTAT GCGTCCTACA GCAGGAGCCA CAGGTGAAAG TGGAAGTGGT 
ACAAGCCTGA TGGTTCTGCC TCCCCCAGCT CCCTCAGTAG ATGCAGATGA TGAGTTTGTG 
TTTGCTGATC CTCTTCCTCC ACCTCTAGAG TTTGCCAATA GTTTTGATCG TGGTTTGGGT 
TACTCTGCGA TGATTTCTCA CAATGTTGCA GCCCGTCAGC AGCGTCATCC TCCACCACCC 
CCTCCCCCAC CACCCCCGCC CACTTCGAAA GATTCCTTTA TGACAGGCTT TCCAGCTCAC 
ACACCCCTAC CGTCACCTCA GGCAGGAGAT TCCACAACTT CCAGTCTTAC CTCCTATGAC 
AGTGAGGTTG CAAACCTAAC ACAATCTGCC CCATCGCCCT CCCCATCTTC TCCCAACCCA 
CTACCACCTC ACTCCCCCTC CACCCTTCAG ACATCTGGAC CACCTCCACC TCCCTCTGTA 
TCACCTCCTC CACCACCCAA CAGCTACCAT AGACCTTTTG GCTTATCTCA GTCACAACAT 
TTATACAACA GTACACACAC AACTGGACGC TCCTCCTCTC CCACCCCACC TCTCTCTATT 
CCAGCTCCAC CTGCCTACCG GGGGCCACCA ACAGCATCAT CCACCCCTGC AACTTCTGTA 
TCAATCAGAG GTGCCTCAAC TGTGACCGCA AGCTGTTCCA CAACTGCTTC TACAACTGTC 
ACCTCTCCTA GTACTACTGC TGCTGCCACA CCACACACAC GCATAGCAAC TGTCTCCACA 
GTAGCTACAG GGGGTAGTAG GCGTCCCAGT GCAGAGCATC ATCCACCACC TAGTGCAACC 
AAGAGTGGTG AGTCCCAGGA GACAGTGGTA GATTCTGGGA TTGAGGAGCT GGACAGCCGA 
AGCAGCAGTG ATCATCACTT AGACAGCATT CTTGCACTCA GTGGTAGTGG GGTCAGAGAG 
AGGTTGGAAC GAGCAGGGAT GAGTAGCATA GGTGGAATTG GAGGGAGAGA TGGTGCAACT 
GAGGTTGACA GGACAAGTGG GGCAGACTTT TTAGAGTCTT ACATGAGCTA CCTTGATGGG 
CAGACTTTTG AAATGCACAA TGCCAAAGCC GCTAGCACTG CATCCACCTA CCCAAAAACA 
CAGAGGTACA GAGAAGGCAC CCGGGCTGGA GATCTTCGAC GCCAGACCAG CACAGCACCT 
CCTGCATACC ACCGTCGTAG AGAGGAGGAA GAGGAAGAGG ATGAGGCCGA GGAGGTAGAG 
GAGGAGGATT TGAGTGCCCG TGATGGCTAT GGGATGAGGG AAGGGCCAGG GATTGCTCGC 
TCCTCTGTAA TGTACTTTGA CCGGCCCCGC ACTCCAGAGA TAAAACCACT GTGGGGAGAT 
GGGTCGACTG GAGCATCACA GACCATGGGG GAGGGAGGGG GCCAGATAGT GGGAACATAT 
GTGGAGCCAC GTAAACTACA TCCTCCCATG ACTGGTATGA AAGCAAACAT CATTAATGAA 
CTAAGCACCA AATTACAGCA GAGAAGTAAA GGAACAGAGA ACTGGGGGGC CCAGAGATCA 
TTGAGCAGAC ATAGACACAG GTTCTCAGAG GACTCAACAG GTGCACTGAG TCCTCCCTCT 
GTCCGCTCTA GCTCCCCATC TCCAGTTCAG CTCTCTCAGC CTGCCTTGTC TCCCTCTCCC 
ACTCCTTCCT CCCAGCCACT TTACTCAAAC TGGGCTCGAT CTCCTTCCCC TCAGCCCCCT 
GCTGCCTCAT CCCAGCCCCC TCCCCGATCC CATTCACCTG TTTCCCCTTC ATCATATTCA 
CCATACCCCA CCTCACCCAA ACACCGGCCT GTTTATCGCA CCAAAGCTCT GGAGTTCCAA 
TTTATTCCCA GTCGAGAGTC ACGAAAGTCA GAGTCGCATC ATGCCCAGCG CAGACGAGCA 
CCCAGCCCTT TAATTTCTCC ATCTGACCGT CCCAAGCTTG GCCCTCCCCG CCCCTCTTCC 
CTTCCTATTC TCCCCACTAC ACCCCTCTAT AGCAGCCTGT ATGACCTTCG TGGTTCTATT 
ACTCCACCCT CACCTGCCAA TCCCCTTGGG GACCCCTACT TTTCCCCCTC CCCCCCTCTA 
TTTGCCCCCT CGGGTGCTCC CCCTCCTCCA AATTCCTCTC TGGTGGTCTC ACGGTCCCTG 
TCACCCACTC ACTTCCTGTC AGGGACTTCA TCCCCACCTC TCCACCCCAT TCCTCCACCC 
ACTTGCCTGA GTTACCCCCA TCTTCCACCC CCCTCACCCA CAAAGCCCTT TGCCTCAAAA 
CCTCTCCCAT ACTGGACCAA GTATGATGTT GCAGATTGGC TGGGCTACCT GAACCTGGGT 
GAACACAGGG AGCGATTCTT AGACAATGAA ATTGATGGCA CGCACCTGCC CTCTCTTACC 
AAGGATGACT ACCTGGACCT GGGTGTGACC CGAGTTGGCC ATCGCATGAA CATAGAGAGG 
GCGCTCAGGA GAGTTATGGA CAGAGAACAC TTGGAGTGGG CCACTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297768.1
CDS	825..8195
Translation	MTMPLSPLSSDEEQQRMLGNSQHLYPGAEEEEEEEEEMDDDGMDEEGEEENGELEMDDEEEVDDEDDIRRGPGHLRGGREEGHRQKGTMAGRLPGDRPIRPGNPGHAANPYSSNPGPTHQTSTNQQQQQPQRKLRERSSSNAPDDTHVALMVFRIGIPDIKQTKCLRFNPDATVWKAKQQVMCSLTESLRDVLNYGLFQPATDGHDAKFLEEERPLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKASLKKFMDYIQTSAVEKVAKLIDKGLDPNYHDPDTGETPLTLAVQTEPGGSEIIRVLVMGGAHIDFRARDGLTPLHKAVRAHAHTALLTLLSLGASSDYKDSRGLTPLYHTVLIGGDTSCCETLLYHRARLGLRDENGWDETHQACQNGNSQHLEHLLFYGADSSSQNASGNTALHICALYNKESCARILLYRGAKKDTKNNSGQNSFQVAVMSGHFELGEIIKNHRESDVVPFLESPKYAPQRRESSRTLGLPHPHPFLRANSDNSMNVPDWIAFPNAAGSSIVSVQGLKHGGTLRSSSSPRGARTRSPSRGRTGDRDDRSRQTRGRQGAGSISSQGTASGQRRRLYSAVPGRVFVATRSHSAQGDREISFNKGDKVKGVDKRCLLLSVGEGGYWEGTVRGRTGWFPSDCVEEVALRSLESRSESRSERAKRLFRHYTVGSYDSFDAPSDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMVDRASAPWKKKSEFESSQATEKKRTVYQMALNKLDEILAAAQQTINTNEAPGPRVQGVKRERGRGFYNEPNFDQTGMGMTSSGAGLGYDRGQFVSGHGPPHGMLRQKSIGVPEEEKQFLHPPAMKFARSLSVPGPDEIPPPPTTAPPEPPFSSAPPLGFRGKTSQQPSVSMSQSTSSAAHYQLYSQSVHVTHGPRDRSPGPPTGSGTGDLAYSHAQAHPSVPSRTASRKVIGYTGDLAGAGGVAGAKAAALRRGYSNAVPPTSVATVIPQQQQTTQNQPSRMDRSGMGVGAGGVPKGGARRGKGPLVKQSKVEDLRQGQDTLEAKVSVEKSSIPIPTIIVKAPSTSSSGRSSQGSSVEAEPPPPGESDSSKPEPVVPPPAPTVPPPAPPSIPAPPPPSLPSLRTQESLDFTSQFGAAIVGAARRDRERFHEARRKSASFFLSAEEELSGAGGAGGRTQTSQQQLSSQPSPRLRPSKSIDEGMFSGDTFIHHTRSMPPAFGLPEYSSPAPQGDYQPKSVPADFYGAGSRQQQQQQQQQAPTTTFIHPLTGKVLDPSSPLGLALAARERALKDDGRIRRERTTEHHFTRQLSSIGAFSSSTSQPTSHISYPMASSSSATSSAAQSTNTSSYLVTSSSLAATTTSTRPPSPRILRGAGSSWSEEAGVGERADREAAGTSAPREGLRVRFSEDKTVHTHHYQSQQYQPTFKERERERQRERSREREREREREREIEKAQTQYQQQSSQPMTQQPRRPSFLRMESEAGAYILSLTPPSPPPTAAPMRPTAGATGESGSGTSLMVLPPPAPSVDADDEFVFADPLPPPLEFANSFDRGLGYSAMISHNVAARQQRHPPPPPPPPPPPTSKDSFMTGFPAHTPLPSPQAGDSTTSSLTSYDSEVANLTQSAPSPSPSSPNPLPPHSPSTLQTSGPPPPPSVSPPPPPNSYHRPFGLSQSQHLYNSTHTTGRSSSPTPPLSIPAPPAYRGPPTASSTPATSVSIRGASTVTASCSTTASTTVTSPSTTAAATPHTRIATVSTVATGGSRRPSAEHHPPPSATKSGESQETVVDSGIEELDSRSSSDHHLDSILALSGSGVRERLERAGMSSIGGIGGRDGATEVDRTSGADFLESYMSYLDGQTFEMHNAKAASTASTYPKTQRYREGTRAGDLRRQTSTAPPAYHRRREEEEEEDEAEEVEEEDLSARDGYGMREGPGIARSSVMYFDRPRTPEIKPLWGDGSTGASQTMGEGGGQIVGTYVEPRKLHPPMTGMKANIINELSTKLQQRSKGTENWGAQRSLSRHRHRFSEDSTGALSPPSVRSSSPSPVQLSQPALSPSPTPSSQPLYSNWARSPSPQPPAASSQPPPRSHSPVSPSSYSPYPTSPKHRPVYRTKALEFQFIPSRESRKSESHHAQRRRAPSPLISPSDRPKLGPPRPSSLPILPTTPLYSSLYDLRGSITPPSPANPLGDPYFSPSPPLFAPSGAPPPPNSSLVVSRSLSPTHFLSGTSSPPLHPIPPPTCLSYPHLPPPSPTKPFASKPLPYWTKYDVADWLGYLNLGEHRERFLDNEIDGTHLPSLTKDDYLDLGVTRVGHRMNIERALRRVMDREHLEWATA

Target ORF information:

RefSeq Version	XM_009299493.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299493.2

ORF Insert Sequence:

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGGTGTGGA TAAACGTTGT 
CTGTTGCTGA GTGTGGGTGA AGGAGGCTAT TGGGAAGGGA CGGTTCGAGG ACGCACAGGC 
TGGTTCCCCT CAGACTGCGT GGAGGAAGTT GCTCTCCGTA GCCTGGAATC TCGTTCAGAG 
AGCCGCAGTG AGAGAGCCAA GAGACTCTTC CGGCATTATA CTGTTGGATC CTACGACAGC 
TTCGATGCGC CCAGTGACTA CATAATCAAG GAGAAGACAG TCCTCCTGCA GAAAAAAGAC 
AATGAAGGTT TTGGCTTTGT GCTGAGAGGA GCCAAGGCTC AGACCCCTAT AGAAGAGTTC 
ACTCCCACCC CAGCCTTTCC AGCACTGCAG TACCTTGAGT CTGTCGACGA GGGAGGCGTG 
GCCTGGAGAG CTGGTCTGCG AATGGGAGAC TTCCTTATAG AGGTGAATGG TCAGAATGTG 
GTTAAGGTGG GACACAGGCA GGTGGTCAAT ATGATCAGGC AAGGCGGTAA CAGTCTCATG 
GTCAAGGTTG TCATGGTTAC TCGAAATCCT GATATGGAGG AAGGGGTCAG AAAGAAAATC 
CCACAGCAGA GTAAAAGACT GAGCACACCG GCTATCGCTC TGCGCTCCAA ATCCATGACA 
TCAGAGCTGG AAGAGATGGT GGACAGAGCC TCTGCCCCAT GGAAAAAAAA ATCAGAGTTC 
GAATCTTCAC AAGCTACAGA GAAGAAGAGG ACAGTTTATC AGATGGCTTT GAATAAACTG 
GATGAGATTT TAGCAGCAGC ACAGCAGACC ATTAATACAA ATGAGGCTCC AGGGCCCCGG 
GTTCAGGGGG TAAAAAGAGA ACGGGGCAGA GGCTTTTATA ATGAGCCAAA TTTCGACCAA 
ACTGGAATGG GGATGACATC ATCTGGGGCA GGGTTAGGCT ACGACCGTGG TCAATTTGTG 
TCTGGCCATG GCCCCCCGCA CGGTATGCTG CGACAGAAGT CTATCGGGGT TCCTGAGGAG 
GAGAAGCAGT TTCTTCATCC TCCAGCCATG AAGTTTGCCC GTAGTCTTTC AGTACCTGGC 
CCAGATGAAA TCCCTCCTCC ACCTACTACA GCACCCCCTG AGCCCCCTTT TTCCTCAGCT 
CCTCCGCTTG GCTTTAGGGG CAAGACATCT CAACAGCCGT CTGTTTCTAT GTCTCAGTCT 
ACTTCCTCTG CTGCACATTA TCAACTCTAC TCACAGTCAG TACATGTTAC TCACGGACCA 
AGGGACAGGT CCCCTGGACC ACCTACTGGG TCTGGAACTG GGGACCTTGC TTATTCCCAT 
GCCCAAGCCC ACCCCTCTGT ACCAAGTAGG ACTGCCTCCA GGAAGGTGAT TGGGTACACA 
GGAGATCTTG CAGGGGCTGG AGGAGTGGCT GGGGCCAAAG CTGCAGCATT GAGAAGGGGT 
TACAGCAATG CAGTCCCACC CACCAGTGTC GCTACTGTGA TTCCACAACA GCAGCAGACT 
ACTCAGAACC AACCTTCCAG AATGGACCGG AGTGGAATGG GGGTAGGGGC TGGTGGTGTA 
CCAAAAGGCG GTGCTCGTAG AGGAAAGGGG CCCTTGGTGA AACAGTCAAA GGTGGAAGAC 
CTGCGTCAAG GTCAAGACAC TCTAGAAGCA AAGGTGTCAG TGGAGAAGAG CTCTATTCCA 
ATCCCAACTA TCATTGTTAA AGCCCCTTCC ACCAGCAGTA GTGGACGCAG TAGCCAAGGC 
AGCAGTGTGG AGGCTGAACC ACCGCCCCCA GGAGAAAGTG ACAGTTCTAA ACCAGAACCT 
GTTGTTCCCC CACCTGCACC AACCGTGCCA CCACCAGCCC CTCCATCTAT ACCTGCTCCT 
CCACCCCCAT CACTGCCATC ACTTCGTACT CAAGAGAGTC TGGACTTCAC CAGCCAGTTT 
GGTGCTGCCA TTGTAGGGGC AGCTCGCAGG GACAGAGAAC GATTTCATGA AGCACGACGC 
AAGAGTGCCT CCTTTTTTCT TTCTGCTGAG GAAGAGTTGA GTGGAGCTGG AGGAGCAGGA 
GGGCGGACTC AGACTTCTCA ACAGCAGCTG AGTTCCCAGC CTTCACCACG CCTGCGACCA 
TCCAAATCTA TAGATGAAGG CATGTTCTCA GGTGATACTT TTATCCATCA CACACGCAGT 
ATGCCTCCTG CATTTGGCTT GCCTGAGTAC TCCTCACCTG CTCCTCAAGG GGATTACCAG 
CCTAAGTCTG TACCTGCTGA CTTCTATGGG GCAGGATCCA GACAACAGCA GCAGCAGCAG 
CAGCAGCAAG CCCCCACCAC AACTTTTATT CACCCACTAA CTGGAAAAGT ACTGGACCCT 
TCATCCCCTC TAGGCTTGGC TTTGGCTGCA CGGGAACGTG CCTTGAAGGA TGATGGACGT 
ATACGTAGGG AAAGGACCAC TGAACATCAT TTTACCCGTC AGTTGTCAAG CATTGGGGCC 
TTTTCCTCAT CCACTTCACA GCCAACATCA CACATTTCCT ACCCAATGGC CTCGTCTTCC 
TCTGCAACCT CCTCTGCTGC TCAATCCACA AACACCTCTT CATATCTTGT TACCTCCTCA 
TCATTAGCTG CCACCACCAC ATCCACTCGG CCTCCATCTC CTAGGATCCT GCGTGGAGCA 
GGGAGTAGCT GGAGTGAAGA AGCTGGGGTT GGAGAGCGTG CGGATAGGGA AGCAGCTGGC 
ACCTCTGCTC CCAGAGAAGG CCTAAGGGTT CGTTTCTCCG AGGATAAAAC TGTCCACACA 
CACCACTATC AATCTCAGCA ATATCAGCCC ACCTTTAAAG AGCGTGAAAG AGAACGTCAA 
AGAGAGAGGT CAAGAGAAAG AGAGAGGGAA AGAGAGAGGG AAAGAGAAAT AGAAAAAGCT 
CAAACCCAGT ATCAGCAACA GTCATCACAA CCAATGACCC AGCAGCCTCG ACGCCCCTCA 
TTCTTGAGGA TGGAGAGTGA AGCTGGAGCG TACATTCTTT CATTAACGCC TCCATCTCCT 
CCTCCAACAG CAGCCCCTAT GCGTCCTACA GCAGGAGCCA CAGGTGAAAG TGGAAGTGGT 
ACAAGCCTGA TGGTTCTGCC TCCCCCAGCT CCCTCAGTAG ATGCAGATGA TGAGTTTGTG 
TTTGCTGATC CTCTTCCTCC ACCTCTAGAG TTTGCCAATA GTTTTGATCG TGGTTTGGGT 
TACTCTGCGA TGATTTCTCA CAATGTTGCA GCCCGTCAGC AGCGTCATCC TCCACCACCC 
CCTCCCCCAC CACCCCCGCC CACTTCGAAA GATTCCTTTA TGACAGGCTT TCCAGCTCAC 
ACACCCCTAC CGTCACCTCA GGCAGGAGAT TCCACAACTT CCAGTCTTAC CTCCTATGAC 
AGTGAGGTTG CAAACCTAAC ACAATCTGCC CCATCGCCCT CCCCATCTTC TCCCAACCCA 
CTACCACCTC ACTCCCCCTC CACCCTTCAG ACATCTGGAC CACCTCCACC TCCCTCTGTA 
TCACCTCCTC CACCACCCAA CAGCTACCAT AGACCTTTTG GCTTATCTCA GTCACAACAT 
TTATACAACA GTACACACAC AACTGGACGC TCCTCCTCTC CCACCCCACC TCTCTCTATT 
CCAGCTCCAC CTGCCTACCG GGGGCCACCA ACAGCATCAT CCACCCCTGC AACTTCTGTA 
TCAATCAGAG GTGCCTCAAC TGTGACCGCA AGCTGTTCCA CAACTGCTTC TACAACTGTC 
ACCTCTCCTA GTACTACTGC TGCTGCCACA CCACACACAC GCATAGCAAC TGTCTCCACA 
GTAGCTACAG GGGGTAGTAG GCGTCCCAGT GCAGAGCATC ATCCACCACC TAGTGCAACC 
AAGAGTGGTG AGTCCCAGGA GACAGTGGTA GATTCTGGGA TTGAGGAGCT GGACAGCCGA 
AGCAGCAGTG ATCATCACTT AGACAGCATT CTTGCACTCA GTGGTAGTGG GGTCAGAGAG 
AGGTTGGAAC GAGCAGGGAT GAGTAGCATA GGTGGAATTG GAGGGAGAGA TGGTGCAACT 
GAGGTTGACA GGACAAGTGG GGCAGACTTT TTAGAGTCTT ACATGAGCTA CCTTGATGGG 
CAGACTTTTG AAATGCACAA TGCCAAAGCC GCTAGCACTG CATCCACCTA CCCAAAAACA 
CAGAGGTACA GAGAAGGCAC CCGGGCTGGA GATCTTCGAC GCCAGACCAG CACAGCACCT 
CCTGCATACC ACCGTCGTAG AGAGGAGGAA GAGGAAGAGG ATGAGGCCGA GGAGGTAGAG 
GAGGAGGATT TGAGTGCCCG TGATGGCTAT GGGATGAGGG AAGGGCCAGG GATTGCTCGC 
TCCTCTGTAA TGTACTTTGA CCGGCCCCGC ACTCCAGAGA TAAAACCACT GTGGGGAGAT 
GGGTCGACTG GAGCATCACA GACCATGGGG GAGGGAGGGG GCCAGATAGT GGGAACATAT 
GTGGAGCCAC GTAAACTACA TCCTCCCATG ACTGGTATGA AAGCAAACAT CATTAATGAA 
CTAAGCACCA AATTACAGCA GAGAAGTAAA GGAACAGAGA ACTGGGGGGC CCAGAGATCA 
TTGAGCAGAC ATAGACACAG GTTCTCAGAG GACTCAACAG GTGCACTGAG TCCTCCCTCT 
GTCCGCTCTA GCTCCCCATC TCCAGTTCAG CTCTCTCAGC CTGCCTTGTC TCCCTCTCCC 
ACTCCTTCCT CCCAGCCACT TTACTCAAAC TGGGCTCGAT CTCCTTCCCC TCAGCCCCCT 
GCTGCCTCAT CCCAGCCCCC TCCCCGATCC CATTCACCTG TTTCCCCTTC ATCATATTCA 
CCATACCCCA CCTCACCCAA ACACCGGCCT GTTTATCGCA CCAAAGCTCT GGAGTTCCAA 
TTTATTCCCA GTCGAGAGTC ACGAAAGTCA GAGTCGCATC ATGCCCAGCG CAGACGAGCA 
CCCAGCCCTT TAATTTCTCC ATCTGACCGT CCCAAGCTTG GCCCTCCCCG CCCCTCTTCC 
CTTCCTATTC TCCCCACTAC ACCCCTCTAT AGCAGCCTGT ATGACCTTCG TGGTTCTATT 
ACTCCACCCT CACCTGCCAA TCCCCTTGGG GACCCCTACT TTTCCCCCTC CCCCCCTCTA 
TTTGCCCCCT CGGGTGCTCC CCCTCCTCCA AATTCCTCTC TGGTGGTCTC ACGGTCCCTG 
TCACCCACTC ACTTCCTGTC AGGGACTTCA TCCCCACCTC TCCACCCCAT TCCTCCACCC 
ACTTGCCTGA GTTACCCCCA TCTTCCACCC CCCTCACCCA CAAAGCCCTT TGCCTCAAAA 
CCTCTCCCAT ACTGGACCAA GTATGATGTT GCAGATTGGC TGGGCTACCT GAACCTGGGT 
GAACACAGGG AGCGATTCTT AGACAATGAA ATTGATGGCA CGCACCTGCC CTCTCTTACC 
AAGGATGACT ACCTGGACCT GGGTGTGACC CGAGTTGGCC ATCGCATGAA CATAGAGAGG 
GCGCTCAGGA GAGTTATGGA CAGAGAACAC TTGGAGTGGG CCACTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_009299496.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297771.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	ODa45599
Clone ID Related Accession (Same CDS sequence)	XM_009299496.2
Accession Version	XM_009299496.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299496.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGGTGTGGA TAAACGTTGT 
CTGTTGCTGA GTGTGGGTGA AGGAGGCTAT TGGGAAGGGA CGGTTCGAGG ACGCACAGGC 
TGGTTCCCCT CAGACTGCGT GGAGGAAGTT GCTCTCCGTA GCCTGGAATC TCGTTCAGAG 
AGCCGCAGTG AGAGAGCCAA GAGACTCTTC CGGCATTATA CTGTTGGATC CTACGACAGC 
TTCGATGCGC CCAGTGACTA CATAATCAAG GAGAAGACAG TCCTCCTGCA GAAAAAAGAC 
AATGAAGGTT TTGGCTTTGT GCTGAGAGGA GCCAAGGCTC AGACCCCTAT AGAAGAGTTC 
ACTCCCACCC CAGCCTTTCC AGCACTGCAG TACCTTGAGT CTGTCGACGA GGGAGGCGTG 
GCCTGGAGAG CTGGTCTGCG AATGGGAGAC TTCCTTATAG AGGTGAATGG TCAGAATGTG 
GTTAAGGTGG GACACAGGCA GGTGGTCAAT ATGATCAGGC AAGGCGGTAA CAGTCTCATG 
GTCAAGGTTG TCATGGTTAC TCGAAATCCT GATATGGAGG AAGGGGTCAG AAAGAAAATC 
CCACAGCAGA GTAAAAGACT GAGCACACCG GCTATCGCTC TGCGCTCCAA ATCCATGACA 
TCAGAGCTGG AAGAGATGGA AGTTCCACAC TGTCACTACC AGCACCCCCA CTACAGTGGA 
CAGAGCCTCT GCCCCATGGA AAAAAAAATC AGAGTTCGAA TCTTCACAAG CTACAGAGAA 
GAAGAGGACA GTTTATCAGA TGGCTTTGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297771.1
CDS	832..3504
Translation	MTMPLSPLSSDEEQQRMLGNSQHLYPGAEEEEEEEEEMDDDGMDEEGEEENGELEMDDEEEVDDEDDIRRGPGHLRGGREEGHRQKGTMAGRLPGDRPIRPGNPGHAANPYSSNPGPTHQTSTNQQQQQPQRKLRERSSSNAPDDTHVALMVFRIGIPDIKQTKCLRFNPDATVWKAKQQVMCSLTESLRDVLNYGLFQPATDGHDAKFLEEERPLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKASLKKFMDYIQTSAVEKVAKLIDKGLDPNYHDPDTGETPLTLAVQTEPGGSEIIRVLVMGGAHIDFRARDGLTPLHKAVRAHAHTALLTLLSLGASSDYKDSRGLTPLYHTVLIGGDTSCCETLLYHRARLGLRDENGWDETHQACQNGNSQHLEHLLFYGADSSSQNASGNTALHICALYNKESCARILLYRGAKKDTKNNSGQNSFQVAVMSGHFELGEIIKNHRESDVVPFLESPKYAPQRRESSRTLGLPHPHPFLRANSDNSMNVPDWIAFPNAAGSSIVSVQGLKHGGTLRSSSSPRGARTRSPSRGRTGDRDDRSRQTRGRQGAGSISSQGTASGQRRRLYSAVPGRVFVATRSHSAQGDREISFNKGDKVKGVDKRCLLLSVGEGGYWEGTVRGRTGWFPSDCVEEVALRSLESRSESRSERAKRLFRHYTVGSYDSFDAPSDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMEVPHCHYQHPHYSGQSLCPMEKKIRVRIFTSYREEEDSLSDGFE

Target ORF information:

RefSeq Version	XM_009299496.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299496.2

ORF Insert Sequence:

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGGTGTGGA TAAACGTTGT 
CTGTTGCTGA GTGTGGGTGA AGGAGGCTAT TGGGAAGGGA CGGTTCGAGG ACGCACAGGC 
TGGTTCCCCT CAGACTGCGT GGAGGAAGTT GCTCTCCGTA GCCTGGAATC TCGTTCAGAG 
AGCCGCAGTG AGAGAGCCAA GAGACTCTTC CGGCATTATA CTGTTGGATC CTACGACAGC 
TTCGATGCGC CCAGTGACTA CATAATCAAG GAGAAGACAG TCCTCCTGCA GAAAAAAGAC 
AATGAAGGTT TTGGCTTTGT GCTGAGAGGA GCCAAGGCTC AGACCCCTAT AGAAGAGTTC 
ACTCCCACCC CAGCCTTTCC AGCACTGCAG TACCTTGAGT CTGTCGACGA GGGAGGCGTG 
GCCTGGAGAG CTGGTCTGCG AATGGGAGAC TTCCTTATAG AGGTGAATGG TCAGAATGTG 
GTTAAGGTGG GACACAGGCA GGTGGTCAAT ATGATCAGGC AAGGCGGTAA CAGTCTCATG 
GTCAAGGTTG TCATGGTTAC TCGAAATCCT GATATGGAGG AAGGGGTCAG AAAGAAAATC 
CCACAGCAGA GTAAAAGACT GAGCACACCG GCTATCGCTC TGCGCTCCAA ATCCATGACA 
TCAGAGCTGG AAGAGATGGA AGTTCCACAC TGTCACTACC AGCACCCCCA CTACAGTGGA 
CAGAGCCTCT GCCCCATGGA AAAAAAAATC AGAGTTCGAA TCTTCACAAG CTACAGAGAA 
GAAGAGGACA GTTTATCAGA TGGCTTTGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shank1 ( XM_009299497.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297772.1 Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	ODa45600
Clone ID Related Accession (Same CDS sequence)	XM_009299497.2
Accession Version	XM_009299497.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	SH3 and multiple ankyrin repeat domains protein 1 isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299497.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGGTGTGGA TAAACGTTGT 
CTGTTGCTGA GTGTGGGTGA AGGAGGCTAT TGGGAAGGGA CGGTTCGAGG ACGCACAGGC 
TGGTTCCCCT CAGACTGCGT GGAGGAAGTT GCTCTCCGTA GCCTGGAATC TCGTTCAGAG 
AGCCGCAGTG AGAGAGCCAA GAGACTCTTC CGGCATTATA CTGTTGGATC CTACGACAGC 
TTCGATGCGC CCAGTGACTA CATAATCAAG GAGAAGACAG TCCTCCTGCA GAAAAAAGAC 
AATGAAGGTT TTGGCTTTGT GCTGAGAGGA GCCAAGGCTC AGACCCCTAT AGAAGAGTTC 
ACTCCCACCC CAGCCTTTCC AGCACTGCAG TACCTTGAGT CTGTCGACGA GGGAGGCGTG 
GCCTGGAGAG CTGGTCTGCG AATGGGAGAC TTCCTTATAG AGGTGAATGG TCAGAATGTG 
GTTAAGGTGG GACACAGGCA GGTGGTCAAT ATGATCAGGC AAGGCGGTAA CAGTCTCATG 
GTCAAGGTTG TCATGGTTAC TCGAAATCCT GATATGGAGG AAGGGGTCAG AAAGAAAATC 
CCACAGCAGA GTAAAAGACT GAGCACACCG GCTATCGCTC TGCGCTCCAA ATCCATGACA 
TCAGAGCTGG AAGAGATGGT TCCACACTGT CACTACCAGC ACCCCCACTA CAGTGGACAG 
AGCCTCTGCC CCATGGAAAA AAAAATCAGA GTTCGAATCT TCACAAGCTA CAGAGAAGAA 
GAGGACAGTT TATCAGATGG CTTTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297772.1
CDS	832..3501
Translation	MTMPLSPLSSDEEQQRMLGNSQHLYPGAEEEEEEEEEMDDDGMDEEGEEENGELEMDDEEEVDDEDDIRRGPGHLRGGREEGHRQKGTMAGRLPGDRPIRPGNPGHAANPYSSNPGPTHQTSTNQQQQQPQRKLRERSSSNAPDDTHVALMVFRIGIPDIKQTKCLRFNPDATVWKAKQQVMCSLTESLRDVLNYGLFQPATDGHDAKFLEEERPLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKASLKKFMDYIQTSAVEKVAKLIDKGLDPNYHDPDTGETPLTLAVQTEPGGSEIIRVLVMGGAHIDFRARDGLTPLHKAVRAHAHTALLTLLSLGASSDYKDSRGLTPLYHTVLIGGDTSCCETLLYHRARLGLRDENGWDETHQACQNGNSQHLEHLLFYGADSSSQNASGNTALHICALYNKESCARILLYRGAKKDTKNNSGQNSFQVAVMSGHFELGEIIKNHRESDVVPFLESPKYAPQRRESSRTLGLPHPHPFLRANSDNSMNVPDWIAFPNAAGSSIVSVQGLKHGGTLRSSSSPRGARTRSPSRGRTGDRDDRSRQTRGRQGAGSISSQGTASGQRRRLYSAVPGRVFVATRSHSAQGDREISFNKGDKVKGVDKRCLLLSVGEGGYWEGTVRGRTGWFPSDCVEEVALRSLESRSESRSERAKRLFRHYTVGSYDSFDAPSDYIIKEKTVLLQKKDNEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNSLMVKVVMVTRNPDMEEGVRKKIPQQSKRLSTPAIALRSKSMTSELEEMVPHCHYQHPHYSGQSLCPMEKKIRVRIFTSYREEEDSLSDGFE

Target ORF information:

RefSeq Version	XM_009299497.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SH3 and multiple ankyrin repeat domains 1 (shank1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299497.2

ORF Insert Sequence:

ATGACGATGC CGCTCAGCCC CCTCTCCAGC GACGAGGAGC AGCAAAGGAT GCTGGGAAAC 
AGTCAACACC TTTATCCAGG GGCGGAAGAG GAAGAGGAGG AGGAAGAGGA AATGGACGAT 
GATGGCATGG ACGAGGAAGG TGAGGAGGAG AACGGAGAGC TGGAGATGGA CGATGAAGAG 
GAAGTGGACG ATGAAGATGA CATCAGGCGA GGACCAGGGC ATTTGAGAGG AGGCAGGGAA 
GAGGGGCACA GGCAAAAAGG CACAATGGCT GGACGACTCC CAGGAGACAG ACCCATTCGA 
CCCGGCAACC CGGGCCATGC GGCCAACCCT TATTCGTCCA ATCCTGGACC AACCCACCAA 
ACGTCAACCA ATCAGCAGCA GCAGCAACCG CAGAGGAAGC TACGAGAGCG GAGCTCCAGC 
AACGCACCTG ACGACACACA CGTAGCTCTG ATGGTCTTTC GAATTGGCAT TCCTGATATT 
AAACAGACGA AATGTCTGAG GTTCAACCCT GATGCAACCG TATGGAAGGC GAAGCAGCAG 
GTGATGTGCA GTCTGACTGA GTCTCTGAGG GATGTTTTAA ACTATGGTCT TTTTCAACCT 
GCCACTGACG GGCACGATGC CAAGTTCCTG GAGGAGGAGA GGCCGCTCCG CGAGTACCCA 
CAATCCTTTG AGAAAGGAGT ACCTTACCTG GAGTTCCGAT ATAAAACCAG GGTGTACAAA 
CAGACAAATC TAGATGAGAA GCAGCTGGCC AAACTACATA CTAAGGCCAG TCTGAAGAAG 
TTCATGGACT ACATCCAGAC CAGCGCTGTG GAAAAGGTAG CCAAACTTAT TGATAAAGGA 
CTTGACCCCA ACTACCACGA CCCTGATACA GGAGAGACTC CACTCACTCT GGCCGTACAG 
ACGGAGCCGG GCGGAAGTGA GATCATCAGG GTGCTGGTGA TGGGTGGAGC CCACATTGAC 
TTTAGGGCGA GAGATGGCTT GACCCCTCTG CACAAGGCTG TCAGAGCACA TGCACACACT 
GCCCTACTGA CGCTGTTGTC TTTGGGGGCG TCTTCTGATT ATAAGGACAG CCGTGGGCTG 
ACACCACTGT ACCACACTGT GCTGATAGGG GGCGACACCT CCTGCTGCGA GACCCTGCTA 
TACCACAGAG CCAGGCTGGG GCTCCGCGAT GAGAACGGCT GGGATGAGAC ACATCAGGCG 
TGTCAAAATG GGAACTCTCA ACATTTAGAG CACCTGCTTT TTTATGGTGC AGACTCCTCA 
TCCCAGAATG CCTCAGGAAA CACTGCCCTG CACATATGTG CTTTATATAA CAAGGAGAGC 
TGTGCCCGCA TCCTGCTTTA TCGAGGAGCT AAGAAGGACA CAAAGAATAA CAGTGGGCAG 
AACTCCTTCC AGGTGGCTGT GATGTCTGGC CATTTTGAGC TTGGAGAAAT AATTAAAAAC 
CACCGTGAAT CAGATGTAGT CCCCTTTTTG GAGTCTCCTA AATATGCCCC GCAAAGGAGG 
GAGAGCTCAC GGACACTTGG ACTGCCTCAT CCGCACCCCT TTTTACGGGC CAACAGTGAT 
AACAGCATGA ATGTGCCAGA CTGGATTGCC TTTCCCAATG CTGCAGGATC AAGCATCGTC 
TCTGTACAGG GTCTAAAGCA TGGCGGCACC TTACGTAGCT CCAGCAGTCC CCGTGGAGCC 
CGAACACGTT CCCCATCTCG TGGCCGAACA GGTGACCGGG ACGATCGGAG CCGGCAAACA 
CGTGGAAGAC AAGGAGCGGG ATCCATCAGT AGTCAGGGCA CAGCCAGCGG GCAGCGGCGG 
CGTCTCTACA GCGCCGTTCC TGGCCGAGTT TTTGTTGCCA CTCGCTCACA CTCGGCCCAG 
GGTGACCGCG AAATCAGCTT CAACAAGGGA GACAAAGTCA AAGGTGTGGA TAAACGTTGT 
CTGTTGCTGA GTGTGGGTGA AGGAGGCTAT TGGGAAGGGA CGGTTCGAGG ACGCACAGGC 
TGGTTCCCCT CAGACTGCGT GGAGGAAGTT GCTCTCCGTA GCCTGGAATC TCGTTCAGAG 
AGCCGCAGTG AGAGAGCCAA GAGACTCTTC CGGCATTATA CTGTTGGATC CTACGACAGC 
TTCGATGCGC CCAGTGACTA CATAATCAAG GAGAAGACAG TCCTCCTGCA GAAAAAAGAC 
AATGAAGGTT TTGGCTTTGT GCTGAGAGGA GCCAAGGCTC AGACCCCTAT AGAAGAGTTC 
ACTCCCACCC CAGCCTTTCC AGCACTGCAG TACCTTGAGT CTGTCGACGA GGGAGGCGTG 
GCCTGGAGAG CTGGTCTGCG AATGGGAGAC TTCCTTATAG AGGTGAATGG TCAGAATGTG 
GTTAAGGTGG GACACAGGCA GGTGGTCAAT ATGATCAGGC AAGGCGGTAA CAGTCTCATG 
GTCAAGGTTG TCATGGTTAC TCGAAATCCT GATATGGAGG AAGGGGTCAG AAAGAAAATC 
CCACAGCAGA GTAAAAGACT GAGCACACCG GCTATCGCTC TGCGCTCCAA ATCCATGACA 
TCAGAGCTGG AAGAGATGGT TCCACACTGT CACTACCAGC ACCCCCACTA CAGTGGACAG 
AGCCTCTGCC CCATGGAAAA AAAAATCAGA GTTCGAATCT TCACAAGCTA CAGAGAAGAA 
GAGGACAGTT TATCAGATGG CTTTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	shank1
Entrez Gene ID	568908
Full Name	SH3 and multiple ankyrin repeat domains 1
Synonyms	si:dkey-105i19.4
General protein information	Preferred Names SH3 and multiple ankyrin repeat domains 1 Names SH3 and multiple ankyrin repeat domains protein 1 zmp:0000000688
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

shank1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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