eif4g3b cDNA ORF clone, Danio rerio(zebrafish)

The following eif4g3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eif4g3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa45533 XM_009296897.3
Latest version!
Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $1021.30
$1459.00
ODa45534 XM_009296898.2
Latest version!
Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $1021.30
$1459.00
ODa45535 XM_009296899.3
Latest version!
Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $1021.30
$1459.00
ODa45536 XM_009296900.2
Latest version!
Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $1021.30
$1459.00
ODa45537 XM_009296901.2
Latest version!
Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $1021.30
$1459.00
ODa146816 XM_021474541.1
Latest version!
Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa146817 XM_021474542.1
Latest version!
Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa146818 XM_021474543.1
Latest version!
Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa146819 XM_021474544.1
Latest version!
Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa146820 XM_021474545.1
Latest version!
Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa146821 XM_021474546.1
Latest version!
Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa146822 XM_021474547.1
Latest version!
Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa151556 XM_021469953.1
Latest version!
Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa151557 XM_021469954.1
Latest version!
Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa151558 XM_021469955.1
Latest version!
Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa151559 XM_021469956.1
Latest version!
Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa151560 XM_021469957.1
Latest version!
Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa27748 XM_001920046.5
Latest version!
Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
View Old Accession Versions >>
ODa45533 XM_009296897.2 Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $1021.30
$1459.00
ODa45535 XM_009296899.2 Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa45533
    Clone ID Related Accession (Same CDS sequence) XM_009296897.2 , XM_009296897.3
    Accession Version XM_009296897.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5151bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296897.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACTAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CAACAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTGCCCCT GGGCCTCGTC CTACCCATCA CCAGGGAGGA
    TTCAGATCCA TTCAGTTTTT CCAGAGGACG CAGATGCAAA CGGCCCGGCC CACCATTCCA
    AGCAACACCC CACCCATTCG GCCCACGTCT CAGACCCCCA CGGCAGCAGT ATATTCTCCC
    AATCAGCACA TCATGATGAC CATGGCTCAC ATGCCCTTTC ATTCCCCACA GACAGCGCAG
    TACTACATCC CTCAGTATCG TCACAGTGCT CCACAGTATG TGGGACCTCC TCAACAGTAC
    CCTGTCCAGC CGACTGGACC CAGTACCTTC TATGCTGCAG CCAGTCCAGG GGAGTTTCCT
    GCACCTTATG CAGCAGGACC TCCCTACTAT CCTGGGCAGC CCGTGTACAC ACCATCTCCA
    CCCATTATAG TGCCTACACC ACAACAACCT CCACCTGCTA AGAGGGAAAA GAAAACAATC
    CGCATCCGAG ACCCTAACCA GGGTGGCAAG GATGTCACAG ACGAAATATT ATCAGGAGTG
    GGTTTGAGTC GAAACCCAAC CCCGCCTGTG GGACGGCCCT CTTCCACTCC TACACCTCCA
    CAGCAGCTGA ACAGCCAGGT AGCAGATCAT GGGCACATCA TGTATAATGT GGACAGCAGC
    CCGCACCTTC CAGCATCCTT CAACTTGAAA GCAGACGATA AGCCAAAACT AGAGTTTTCC
    TTGCAAAGAA CTGCATCTCC TGGTCTGCGG CAGCCCGACA CTCCTCTGGA GAGAAGAGAT
    CCCAGCAGCC CAGTCCAAAC ACCCTCCTCT CCCCCACACA AACCTGAGCT TCCCCCCTCC
    GACTCAGAGA CGGCCTCAAG TGTAGCTACA GCCCCGACTC CTTCCATACC TGCCTCAACA
    GAAGAGTCAG CTGATGCCCC GAGCCCATTA GCTGAGCCCA GTCTCACTAA AGCTATCACA
    CCTGAACCAG AGAGCTCTGA GCCAGAGAAA TCCTCCTCAC CACCACCTCA AAGTCTGTCT
    GGATCCCTTA CCCAGCACGA AAAAGCCGTC AATGGGCTCA CGGATGTTGA CGCTGCTCCA
    CTGAGTGAAG AATTGGAAAC CCAACCCAGG GAAGCCTCTC CATTGCTGCC TACTTCCAGT
    GTGCCCCAGT CAGAGCCCAG GCCAGTCACA CCGGTGCTGG AGGAAGAGTC TGATCCAATC
    AATATGAACA GTCCTTTGCC TCCTGTTGAG GACGATGCTG GATGTCCTGA CAACGTTTCC
    CCCTCCCTCT CCACCTCCAC GACTGCTGCT ATCTCCACCA CTCCTCCTGC TCCTCCTCCA
    GGACTCTCGC ACCCCAGTCA GGTTTCTGCA GCGTTGGACA GGAGACCCTC TAATGGAGCA
    GAGATCAAAG AAACAGGGAA GGAAAACGAG GCCTTGCCGG ACAAGAGAGG AGAGCCTTTC
    TTACAGTCCA GAAAAAGCTC AAATCAAGCT ACATCTAGTG CACCAAAGAC CTGGAAGAAG
    CCAAAAGAGG ACATGCCTGT AGGGCAAGAT CAGTGTCCAG ACAAAGAGGA GGACACTGAT
    CAGGCCAGTA TGGAACCTGC TCCTGAAAGC CCCAGTCCTC CTCCTGTGGA GGTGGAGAGG
    AGGCCATCAG TAGGAGCAGT GCTCGAGGAG AATGGAGAAC AGGAAGTGGA GCCTTTGAGA
    AACGGTGCTG AACACACTTC TGAAACTGAG AGCAGTGACA GCATCCACCC GCCTGCAGTC
    AAAGAGCCGT TGATCAAAGT ATTGCCACAG CCTGCAGAGA AGAATGGAAA GAAGCAGTAC
    AACAGAGACT TCCTGCTGGG CTTCCAGTTT ATGCCTGCAT GTGTGCAGAA ACCAGAAGGG
    CTTCCTCCCA TTTCTGATGT TGTTCTAGAT AAGATTAACC AAAATAAGCT GCCCATGCGG
    CCGGTAGACC CACGTGTGCT TTCCAGAGGC CCGGACTTTA CGCCAGCGTT TGCGGATTTC
    AGCCGACCCA TTTCAGGAGG CAGAGGGGCA CCGTTGCTGA ATGTTGGCAT GCGACGGCCA
    CCTGGACGCA AGATCATCAC AAACGTGTCT GTGATTGATG AGGTGCAGCT GAAGACAACA
    GAGAATGCCT GGAAGCCTGG CATGAAGCGG GAAGGTGCAG TTGAAGATCC AGATGCCCAG
    AGAACTCAGG AGCTTTTCCG AAAAGTGCGC AGTATTCTCA ATAAACTGAC ACCGCAGATG
    TTCAGCCAGC TGATGAAGCA GGTGACTGAC CTCACCATCG ACACAGAGGA GCGACTCAAG
    GGTGTCATAG ATCTGGTCTT TGAAAAAGCA ATCAATGAGC CCAGCTTCTC TGTGGCCTAC
    GGCAACATGT GCAGCTGTCT GGCCACGTTA AAAGTGCCCA TGACGGATAA GCCCAACAGC
    ACTGTGAATT TCCGGAAGCT GCTTCTGAAC CGCTGTCAGA AAGAGTTTGA GAAGGACAAG
    ATGGATGACG ATGCCTTTGA GAAGAAACAC AGGGAGCTGG AGGCAGCTAC TGCGTCCAGT
    GAGCGGGAGA GGCTTCAGGA GGAGCTGGAA GAGGCCAAAG ACAAGGCCCG AAGACGCTCC
    ATTGGCAACA TTAAGTTCAT CGGCGAGCTC TTCAAGCTGC GGATGCTGAC AGAGGCCATC
    ATGCATGACT GCGTGGTGAA GTTGCTGAAA AACCATGATG AGGAATCACT GGAGTGCCTG
    TGTAGACTGC TCACCACTAT TGGAAAGGAC CTGGACTTTG AGAAAGCTAA GCCACGAATG
    GATCAGTACT TTAATCAGAT GGAGAAGATA GTTAAGGAGA GGAAGACTTC TTCACGCATT
    CGCTTCATGC TGCAGGATGT TATTGATCTG CGATTGCACA ACTGGGTTTC TCGAAGAGCA
    GATCAGGGCC CCAAGACCAT CGAGCAGATC CATAAAGACG CAAAGCTGGA GGAGCAGGAG
    GAACAGAGGA AAGTCCACCA GCAACTGCTT TCCAAAGACA ACAAGAGGAG ACCAGTGGTG
    CAGAGAGAGG AGACCTGGAG CACTGTGCCT ATGACTAAGA ACAGCAGAAC AATAGACCCC
    GCCAAGATTC CCAAATTCTC CAAGCCAGCG ATAGATGAGA AGATTCAGCT GGGGCCGAGA
    GCTCAGGTGA ACTGGATGAA GGGCAGCAGT GGTGGAGCTG GAGCAAAGGC CAGTGAGTCA
    GATGCGTCTC GGCCGAGTGC CAGTTTGAAT CGTTACTCTC CTCTGCAACC CTCGGCACTC
    CAGACTTCTT CTTTACCCTC CACATCTCCA GATTTTGATT CCAGGAGAGT TCTCGGCAGT
    CGTGGAAGCT CAGGACGGGA GCGCAATGAC AAGCCATTGA GTGCGGGTCC TGCTCGGACA
    GGGCCTATTT CATTGAGCAG TAGCAATAAG GAGACCCCCG AAGAGCTGGT TCAGGAAGTG
    TCCCGCAGAG ACTCCAATGC TTCAGATACA CCCAAGCTGC TCGTCAGTAC TGCAGACAAG
    AGCAGACTGG AGAACAGCCA ACCCAGAGAG TCTGCTGTTA AACTTGAAGC ACTCTCAGGG
    CCCTCTCCAG ATAAACCTGC ATTATCAGAA GAGGAGATGG AGAGGCGGTC CAAGTCTATA
    ATTGATGAGT TTTTGCACAT TAATGATTAC AAGGAAGCTT TGCAGTGTGT TGAGGAACTA
    GAGCAGTCTG CCATGCTCTA TGTGTTTGTG CGGGTGGGTG TGGAGTCCAC GCTGGAAAGG
    AGTCAGATCA CGCGAGACCA TATGGGTCAG CTGCTCTTCC AGCTTCTGCA GGCTGGAGTC
    CTCCCTAAAC TTCAGTTCTT TAAAGGGTTT TCTGAAACTC TTGAATTAGC AGATGATATG
    GCCATTGATA TTCCCCATAT ATGGCTGTAC CTTGCGGAGC TGGTAACCCC TGTGCTTCGA
    GAGGGGGGAA TCTCTATGAG AGAATTATTT AGTGAATTTA GCAAACCATT ACTCCCTGTG
    GGAAGAGCTG GGATATTATT TTCTGAAATA TTGCACCTTC TATGCAAACA AATGAGCCAT
    AAGAAAGTGG GATCATTATG GAGGGATTCT GGGTTGAGCT GGGCAGATTT TATACCTGAA
    ACAGAGGATG TGCACAGCTT CATCACAGAA CATAAACTAG AGTTCACTCT GTCTGATGGC
    TCAAACCCGA CCGAGGCTGT CTCTAAGAGA ACACTCTCGC CTGAGGAACT GAACAAGCAA
    CTTGAAAAAC TTTTACTTGA GGACATGGTT GGCGATGAAC AAATCTTCGA CTGGGTTGAG
    GCCAATCTAG ATGAATCAGA AATGAGTTCA GCTCCTTTCG TCAGAGCTTT AATGACTGCT
    GTGTGTAAAG CAGCAGTAAA AACTGAGGGC TCCTCGTGTA AAGTGGACTT GTCCATAATT
    CAGACGAGAT TGCCTGTATT ACACAAGTAC CTTAACTCAG ACACAGAGAG ACAGTTGCAA
    GCACTTTATG CACTTCAAGC ATTGATAGTA AAACTGGACC AGCCTGCTAA TTTGCTCCGG
    ATGTTTTTTG ACTGTTTGTA TGATGAGGAC GTGATCTCTG AAGACGCTTT CTATAAATGG
    GAGGTCAGTA AAGACCCCGC TGAACAGCAA GGCAAAGGTG TTGCCCTGAA GTCCGTCACA
    GCCTTCTTCA CGTGGCTGCG CGAGGCCGAG GAAGAGTCCG AGGACAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295172.1
    CDS606..5756
    Translation

    Target ORF information:

    RefSeq Version XM_009296897.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296897.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACTAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CAACAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTGCCCCT GGGCCTCGTC CTACCCATCA CCAGGGAGGA
    TTCAGATCCA TTCAGTTTTT CCAGAGGACG CAGATGCAAA CGGCCCGGCC CACCATTCCA
    AGCAACACCC CACCCATTCG GCCCACGTCT CAGACCCCCA CGGCAGCAGT ATATTCTCCC
    AATCAGCACA TCATGATGAC CATGGCTCAC ATGCCCTTTC ATTCCCCACA GACAGCGCAG
    TACTACATCC CTCAGTATCG TCACAGTGCT CCACAGTATG TGGGACCTCC TCAACAGTAC
    CCTGTCCAGC CGACTGGACC CAGTACCTTC TATGCTGCAG CCAGTCCAGG GGAGTTTCCT
    GCACCTTATG CAGCAGGACC TCCCTACTAT CCTGGGCAGC CCGTGTACAC ACCATCTCCA
    CCCATTATAG TGCCTACACC ACAACAACCT CCACCTGCTA AGAGGGAAAA GAAAACAATC
    CGCATCCGAG ACCCTAACCA GGGTGGCAAG GATGTCACAG ACGAAATATT ATCAGGAGTG
    GGTTTGAGTC GAAACCCAAC CCCGCCTGTG GGACGGCCCT CTTCCACTCC TACACCTCCA
    CAGCAGCTGA ACAGCCAGGT AGCAGATCAT GGGCACATCA TGTATAATGT GGACAGCAGC
    CCGCACCTTC CAGCATCCTT CAACTTGAAA GCAGACGATA AGCCAAAACT AGAGTTTTCC
    TTGCAAAGAA CTGCATCTCC TGGTCTGCGG CAGCCCGACA CTCCTCTGGA GAGAAGAGAT
    CCCAGCAGCC CAGTCCAAAC ACCCTCCTCT CCCCCACACA AACCTGAGCT TCCCCCCTCC
    GACTCAGAGA CGGCCTCAAG TGTAGCTACA GCCCCGACTC CTTCCATACC TGCCTCAACA
    GAAGAGTCAG CTGATGCCCC GAGCCCATTA GCTGAGCCCA GTCTCACTAA AGCTATCACA
    CCTGAACCAG AGAGCTCTGA GCCAGAGAAA TCCTCCTCAC CACCACCTCA AAGTCTGTCT
    GGATCCCTTA CCCAGCACGA AAAAGCCGTC AATGGGCTCA CGGATGTTGA CGCTGCTCCA
    CTGAGTGAAG AATTGGAAAC CCAACCCAGG GAAGCCTCTC CATTGCTGCC TACTTCCAGT
    GTGCCCCAGT CAGAGCCCAG GCCAGTCACA CCGGTGCTGG AGGAAGAGTC TGATCCAATC
    AATATGAACA GTCCTTTGCC TCCTGTTGAG GACGATGCTG GATGTCCTGA CAACGTTTCC
    CCCTCCCTCT CCACCTCCAC GACTGCTGCT ATCTCCACCA CTCCTCCTGC TCCTCCTCCA
    GGACTCTCGC ACCCCAGTCA GGTTTCTGCA GCGTTGGACA GGAGACCCTC TAATGGAGCA
    GAGATCAAAG AAACAGGGAA GGAAAACGAG GCCTTGCCGG ACAAGAGAGG AGAGCCTTTC
    TTACAGTCCA GAAAAAGCTC AAATCAAGCT ACATCTAGTG CACCAAAGAC CTGGAAGAAG
    CCAAAAGAGG ACATGCCTGT AGGGCAAGAT CAGTGTCCAG ACAAAGAGGA GGACACTGAT
    CAGGCCAGTA TGGAACCTGC TCCTGAAAGC CCCAGTCCTC CTCCTGTGGA GGTGGAGAGG
    AGGCCATCAG TAGGAGCAGT GCTCGAGGAG AATGGAGAAC AGGAAGTGGA GCCTTTGAGA
    AACGGTGCTG AACACACTTC TGAAACTGAG AGCAGTGACA GCATCCACCC GCCTGCAGTC
    AAAGAGCCGT TGATCAAAGT ATTGCCACAG CCTGCAGAGA AGAATGGAAA GAAGCAGTAC
    AACAGAGACT TCCTGCTGGG CTTCCAGTTT ATGCCTGCAT GTGTGCAGAA ACCAGAAGGG
    CTTCCTCCCA TTTCTGATGT TGTTCTAGAT AAGATTAACC AAAATAAGCT GCCCATGCGG
    CCGGTAGACC CACGTGTGCT TTCCAGAGGC CCGGACTTTA CGCCAGCGTT TGCGGATTTC
    AGCCGACCCA TTTCAGGAGG CAGAGGGGCA CCGTTGCTGA ATGTTGGCAT GCGACGGCCA
    CCTGGACGCA AGATCATCAC AAACGTGTCT GTGATTGATG AGGTGCAGCT GAAGACAACA
    GAGAATGCCT GGAAGCCTGG CATGAAGCGG GAAGGTGCAG TTGAAGATCC AGATGCCCAG
    AGAACTCAGG AGCTTTTCCG AAAAGTGCGC AGTATTCTCA ATAAACTGAC ACCGCAGATG
    TTCAGCCAGC TGATGAAGCA GGTGACTGAC CTCACCATCG ACACAGAGGA GCGACTCAAG
    GGTGTCATAG ATCTGGTCTT TGAAAAAGCA ATCAATGAGC CCAGCTTCTC TGTGGCCTAC
    GGCAACATGT GCAGCTGTCT GGCCACGTTA AAAGTGCCCA TGACGGATAA GCCCAACAGC
    ACTGTGAATT TCCGGAAGCT GCTTCTGAAC CGCTGTCAGA AAGAGTTTGA GAAGGACAAG
    ATGGATGACG ATGCCTTTGA GAAGAAACAC AGGGAGCTGG AGGCAGCTAC TGCGTCCAGT
    GAGCGGGAGA GGCTTCAGGA GGAGCTGGAA GAGGCCAAAG ACAAGGCCCG AAGACGCTCC
    ATTGGCAACA TTAAGTTCAT CGGCGAGCTC TTCAAGCTGC GGATGCTGAC AGAGGCCATC
    ATGCATGACT GCGTGGTGAA GTTGCTGAAA AACCATGATG AGGAATCACT GGAGTGCCTG
    TGTAGACTGC TCACCACTAT TGGAAAGGAC CTGGACTTTG AGAAAGCTAA GCCACGAATG
    GATCAGTACT TTAATCAGAT GGAGAAGATA GTTAAGGAGA GGAAGACTTC TTCACGCATT
    CGCTTCATGC TGCAGGATGT TATTGATCTG CGATTGCACA ACTGGGTTTC TCGAAGAGCA
    GATCAGGGCC CCAAGACCAT CGAGCAGATC CATAAAGACG CAAAGCTGGA GGAGCAGGAG
    GAACAGAGGA AAGTCCACCA GCAACTGCTT TCCAAAGACA ACAAGAGGAG ACCAGTGGTG
    CAGAGAGAGG AGACCTGGAG CACTGTGCCT ATGACTAAGA ACAGCAGAAC AATAGACCCC
    GCCAAGATTC CCAAATTCTC CAAGCCAGCG ATAGATGAGA AGATTCAGCT GGGGCCGAGA
    GCTCAGGTGA ACTGGATGAA GGGCAGCAGT GGTGGAGCTG GAGCAAAGGC CAGTGAGTCA
    GATGCGTCTC GGCCGAGTGC CAGTTTGAAT CGTTACTCTC CTCTGCAACC CTCGGCACTC
    CAGACTTCTT CTTTACCCTC CACATCTCCA GATTTTGATT CCAGGAGAGT TCTCGGCAGT
    CGTGGAAGCT CAGGACGGGA GCGCAATGAC AAGCCATTGA GTGCGGGTCC TGCTCGGACA
    GGGCCTATTT CATTGAGCAG TAGCAATAAG GAGACCCCCG AAGAGCTGGT TCAGGAAGTG
    TCCCGCAGAG ACTCCAATGC TTCAGATACA CCCAAGCTGC TCGTCAGTAC TGCAGACAAG
    AGCAGACTGG AGAACAGCCA ACCCAGAGAG TCTGCTGTTA AACTTGAAGC ACTCTCAGGG
    CCCTCTCCAG ATAAACCTGC ATTATCAGAA GAGGAGATGG AGAGGCGGTC CAAGTCTATA
    ATTGATGAGT TTTTGCACAT TAATGATTAC AAGGAAGCTT TGCAGTGTGT TGAGGAACTA
    GAGCAGTCTG CCATGCTCTA TGTGTTTGTG CGGGTGGGTG TGGAGTCCAC GCTGGAAAGG
    AGTCAGATCA CGCGAGACCA TATGGGTCAG CTGCTCTTCC AGCTTCTGCA GGCTGGAGTC
    CTCCCTAAAC TTCAGTTCTT TAAAGGGTTT TCTGAAACTC TTGAATTAGC AGATGATATG
    GCCATTGATA TTCCCCATAT ATGGCTGTAC CTTGCGGAGC TGGTAACCCC TGTGCTTCGA
    GAGGGGGGAA TCTCTATGAG AGAATTATTT AGTGAATTTA GCAAACCATT ACTCCCTGTG
    GGAAGAGCTG GGATATTATT TTCTGAAATA TTGCACCTTC TATGCAAACA AATGAGCCAT
    AAGAAAGTGG GATCATTATG GAGGGATTCT GGGTTGAGCT GGGCAGATTT TATACCTGAA
    ACAGAGGATG TGCACAGCTT CATCACAGAA CATAAACTAG AGTTCACTCT GTCTGATGGC
    TCAAACCCGA CCGAGGCTGT CTCTAAGAGA ACACTCTCGC CTGAGGAACT GAACAAGCAA
    CTTGAAAAAC TTTTACTTGA GGACATGGTT GGCGATGAAC AAATCTTCGA CTGGGTTGAG
    GCCAATCTAG ATGAATCAGA AATGAGTTCA GCTCCTTTCG TCAGAGCTTT AATGACTGCT
    GTGTGTAAAG CAGCAGTAAA AACTGAGGGC TCCTCGTGTA AAGTGGACTT GTCCATAATT
    CAGACGAGAT TGCCTGTATT ACACAAGTAC CTTAACTCAG ACACAGAGAG ACAGTTGCAA
    GCACTTTATG CACTTCAAGC ATTGATAGTA AAACTGGACC AGCCTGCTAA TTTGCTCCGG
    ATGTTTTTTG ACTGTTTGTA TGATGAGGAC GTGATCTCTG AAGACGCTTT CTATAAATGG
    GAGGTCAGTA AAGACCCCGC TGAACAGCAA GGCAAAGGTG TTGCCCTGAA GTCCGTCACA
    GCCTTCTTCA CGTGGCTGCG CGAGGCCGAG GAAGAGTCCG AGGACAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45533
    Clone ID Related Accession (Same CDS sequence) XM_009296897.2 , XM_009296897.3
    Accession Version XM_009296897.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5151bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296897.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACTAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CAACAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTGCCCCT GGGCCTCGTC CTACCCATCA CCAGGGAGGA
    TTCAGATCCA TTCAGTTTTT CCAGAGGACG CAGATGCAAA CGGCCCGGCC CACCATTCCA
    AGCAACACCC CACCCATTCG GCCCACGTCT CAGACCCCCA CGGCAGCAGT ATATTCTCCC
    AATCAGCACA TCATGATGAC CATGGCTCAC ATGCCCTTTC ATTCCCCACA GACAGCGCAG
    TACTACATCC CTCAGTATCG TCACAGTGCT CCACAGTATG TGGGACCTCC TCAACAGTAC
    CCTGTCCAGC CGACTGGACC CAGTACCTTC TATGCTGCAG CCAGTCCAGG GGAGTTTCCT
    GCACCTTATG CAGCAGGACC TCCCTACTAT CCTGGGCAGC CCGTGTACAC ACCATCTCCA
    CCCATTATAG TGCCTACACC ACAACAACCT CCACCTGCTA AGAGGGAAAA GAAAACAATC
    CGCATCCGAG ACCCTAACCA GGGTGGCAAG GATGTCACAG ACGAAATATT ATCAGGAGTG
    GGTTTGAGTC GAAACCCAAC CCCGCCTGTG GGACGGCCCT CTTCCACTCC TACACCTCCA
    CAGCAGCTGA ACAGCCAGGT AGCAGATCAT GGGCACATCA TGTATAATGT GGACAGCAGC
    CCGCACCTTC CAGCATCCTT CAACTTGAAA GCAGACGATA AGCCAAAACT AGAGTTTTCC
    TTGCAAAGAA CTGCATCTCC TGGTCTGCGG CAGCCCGACA CTCCTCTGGA GAGAAGAGAT
    CCCAGCAGCC CAGTCCAAAC ACCCTCCTCT CCCCCACACA AACCTGAGCT TCCCCCCTCC
    GACTCAGAGA CGGCCTCAAG TGTAGCTACA GCCCCGACTC CTTCCATACC TGCCTCAACA
    GAAGAGTCAG CTGATGCCCC GAGCCCATTA GCTGAGCCCA GTCTCACTAA AGCTATCACA
    CCTGAACCAG AGAGCTCTGA GCCAGAGAAA TCCTCCTCAC CACCACCTCA AAGTCTGTCT
    GGATCCCTTA CCCAGCACGA AAAAGCCGTC AATGGGCTCA CGGATGTTGA CGCTGCTCCA
    CTGAGTGAAG AATTGGAAAC CCAACCCAGG GAAGCCTCTC CATTGCTGCC TACTTCCAGT
    GTGCCCCAGT CAGAGCCCAG GCCAGTCACA CCGGTGCTGG AGGAAGAGTC TGATCCAATC
    AATATGAACA GTCCTTTGCC TCCTGTTGAG GACGATGCTG GATGTCCTGA CAACGTTTCC
    CCCTCCCTCT CCACCTCCAC GACTGCTGCT ATCTCCACCA CTCCTCCTGC TCCTCCTCCA
    GGACTCTCGC ACCCCAGTCA GGTTTCTGCA GCGTTGGACA GGAGACCCTC TAATGGAGCA
    GAGATCAAAG AAACAGGGAA GGAAAACGAG GCCTTGCCGG ACAAGAGAGG AGAGCCTTTC
    TTACAGTCCA GAAAAAGCTC AAATCAAGCT ACATCTAGTG CACCAAAGAC CTGGAAGAAG
    CCAAAAGAGG ACATGCCTGT AGGGCAAGAT CAGTGTCCAG ACAAAGAGGA GGACACTGAT
    CAGGCCAGTA TGGAACCTGC TCCTGAAAGC CCCAGTCCTC CTCCTGTGGA GGTGGAGAGG
    AGGCCATCAG TAGGAGCAGT GCTCGAGGAG AATGGAGAAC AGGAAGTGGA GCCTTTGAGA
    AACGGTGCTG AACACACTTC TGAAACTGAG AGCAGTGACA GCATCCACCC GCCTGCAGTC
    AAAGAGCCGT TGATCAAAGT ATTGCCACAG CCTGCAGAGA AGAATGGAAA GAAGCAGTAC
    AACAGAGACT TCCTGCTGGG CTTCCAGTTT ATGCCTGCAT GTGTGCAGAA ACCAGAAGGG
    CTTCCTCCCA TTTCTGATGT TGTTCTAGAT AAGATTAACC AAAATAAGCT GCCCATGCGG
    CCGGTAGACC CACGTGTGCT TTCCAGAGGC CCGGACTTTA CGCCAGCGTT TGCGGATTTC
    AGCCGACCCA TTTCAGGAGG CAGAGGGGCA CCGTTGCTGA ATGTTGGCAT GCGACGGCCA
    CCTGGACGCA AGATCATCAC AAACGTGTCT GTGATTGATG AGGTGCAGCT GAAGACAACA
    GAGAATGCCT GGAAGCCTGG CATGAAGCGG GAAGGTGCAG TTGAAGATCC AGATGCCCAG
    AGAACTCAGG AGCTTTTCCG AAAAGTGCGC AGTATTCTCA ATAAACTGAC ACCGCAGATG
    TTCAGCCAGC TGATGAAGCA GGTGACTGAC CTCACCATCG ACACAGAGGA GCGACTCAAG
    GGTGTCATAG ATCTGGTCTT TGAAAAAGCA ATCAATGAGC CCAGCTTCTC TGTGGCCTAC
    GGCAACATGT GCAGCTGTCT GGCCACGTTA AAAGTGCCCA TGACGGATAA GCCCAACAGC
    ACTGTGAATT TCCGGAAGCT GCTTCTGAAC CGCTGTCAGA AAGAGTTTGA GAAGGACAAG
    ATGGATGACG ATGCCTTTGA GAAGAAACAC AGGGAGCTGG AGGCAGCTAC TGCGTCCAGT
    GAGCGGGAGA GGCTTCAGGA GGAGCTGGAA GAGGCCAAAG ACAAGGCCCG AAGACGCTCC
    ATTGGCAACA TTAAGTTCAT CGGCGAGCTC TTCAAGCTGC GGATGCTGAC AGAGGCCATC
    ATGCATGACT GCGTGGTGAA GTTGCTGAAA AACCATGATG AGGAATCACT GGAGTGCCTG
    TGTAGACTGC TCACCACTAT TGGAAAGGAC CTGGACTTTG AGAAAGCTAA GCCACGAATG
    GATCAGTACT TTAATCAGAT GGAGAAGATA GTTAAGGAGA GGAAGACTTC TTCACGCATT
    CGCTTCATGC TGCAGGATGT TATTGATCTG CGATTGCACA ACTGGGTTTC TCGAAGAGCA
    GATCAGGGCC CCAAGACCAT CGAGCAGATC CATAAAGACG CAAAGCTGGA GGAGCAGGAG
    GAACAGAGGA AAGTCCACCA GCAACTGCTT TCCAAAGACA ACAAGAGGAG ACCAGTGGTG
    CAGAGAGAGG AGACCTGGAG CACTGTGCCT ATGACTAAGA ACAGCAGAAC AATAGACCCC
    GCCAAGATTC CCAAATTCTC CAAGCCAGCG ATAGATGAGA AGATTCAGCT GGGGCCGAGA
    GCTCAGGTGA ACTGGATGAA GGGCAGCAGT GGTGGAGCTG GAGCAAAGGC CAGTGAGTCA
    GATGCGTCTC GGCCGAGTGC CAGTTTGAAT CGTTACTCTC CTCTGCAACC CTCGGCACTC
    CAGACTTCTT CTTTACCCTC CACATCTCCA GATTTTGATT CCAGGAGAGT TCTCGGCAGT
    CGTGGAAGCT CAGGACGGGA GCGCAATGAC AAGCCATTGA GTGCGGGTCC TGCTCGGACA
    GGGCCTATTT CATTGAGCAG TAGCAATAAG GAGACCCCCG AAGAGCTGGT TCAGGAAGTG
    TCCCGCAGAG ACTCCAATGC TTCAGATACA CCCAAGCTGC TCGTCAGTAC TGCAGACAAG
    AGCAGACTGG AGAACAGCCA ACCCAGAGAG TCTGCTGTTA AACTTGAAGC ACTCTCAGGG
    CCCTCTCCAG ATAAACCTGC ATTATCAGAA GAGGAGATGG AGAGGCGGTC CAAGTCTATA
    ATTGATGAGT TTTTGCACAT TAATGATTAC AAGGAAGCTT TGCAGTGTGT TGAGGAACTA
    GAGCAGTCTG CCATGCTCTA TGTGTTTGTG CGGGTGGGTG TGGAGTCCAC GCTGGAAAGG
    AGTCAGATCA CGCGAGACCA TATGGGTCAG CTGCTCTTCC AGCTTCTGCA GGCTGGAGTC
    CTCCCTAAAC TTCAGTTCTT TAAAGGGTTT TCTGAAACTC TTGAATTAGC AGATGATATG
    GCCATTGATA TTCCCCATAT ATGGCTGTAC CTTGCGGAGC TGGTAACCCC TGTGCTTCGA
    GAGGGGGGAA TCTCTATGAG AGAATTATTT AGTGAATTTA GCAAACCATT ACTCCCTGTG
    GGAAGAGCTG GGATATTATT TTCTGAAATA TTGCACCTTC TATGCAAACA AATGAGCCAT
    AAGAAAGTGG GATCATTATG GAGGGATTCT GGGTTGAGCT GGGCAGATTT TATACCTGAA
    ACAGAGGATG TGCACAGCTT CATCACAGAA CATAAACTAG AGTTCACTCT GTCTGATGGC
    TCAAACCCGA CCGAGGCTGT CTCTAAGAGA ACACTCTCGC CTGAGGAACT GAACAAGCAA
    CTTGAAAAAC TTTTACTTGA GGACATGGTT GGCGATGAAC AAATCTTCGA CTGGGTTGAG
    GCCAATCTAG ATGAATCAGA AATGAGTTCA GCTCCTTTCG TCAGAGCTTT AATGACTGCT
    GTGTGTAAAG CAGCAGTAAA AACTGAGGGC TCCTCGTGTA AAGTGGACTT GTCCATAATT
    CAGACGAGAT TGCCTGTATT ACACAAGTAC CTTAACTCAG ACACAGAGAG ACAGTTGCAA
    GCACTTTATG CACTTCAAGC ATTGATAGTA AAACTGGACC AGCCTGCTAA TTTGCTCCGG
    ATGTTTTTTG ACTGTTTGTA TGATGAGGAC GTGATCTCTG AAGACGCTTT CTATAAATGG
    GAGGTCAGTA AAGACCCCGC TGAACAGCAA GGCAAAGGTG TTGCCCTGAA GTCCGTCACA
    GCCTTCTTCA CGTGGCTGCG CGAGGCCGAG GAAGAGTCCG AGGACAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295172.1
    CDS458..5608
    Translation

    Target ORF information:

    RefSeq Version XM_009296897.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296897.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACTAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CAACAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTGCCCCT GGGCCTCGTC CTACCCATCA CCAGGGAGGA
    TTCAGATCCA TTCAGTTTTT CCAGAGGACG CAGATGCAAA CGGCCCGGCC CACCATTCCA
    AGCAACACCC CACCCATTCG GCCCACGTCT CAGACCCCCA CGGCAGCAGT ATATTCTCCC
    AATCAGCACA TCATGATGAC CATGGCTCAC ATGCCCTTTC ATTCCCCACA GACAGCGCAG
    TACTACATCC CTCAGTATCG TCACAGTGCT CCACAGTATG TGGGACCTCC TCAACAGTAC
    CCTGTCCAGC CGACTGGACC CAGTACCTTC TATGCTGCAG CCAGTCCAGG GGAGTTTCCT
    GCACCTTATG CAGCAGGACC TCCCTACTAT CCTGGGCAGC CCGTGTACAC ACCATCTCCA
    CCCATTATAG TGCCTACACC ACAACAACCT CCACCTGCTA AGAGGGAAAA GAAAACAATC
    CGCATCCGAG ACCCTAACCA GGGTGGCAAG GATGTCACAG ACGAAATATT ATCAGGAGTG
    GGTTTGAGTC GAAACCCAAC CCCGCCTGTG GGACGGCCCT CTTCCACTCC TACACCTCCA
    CAGCAGCTGA ACAGCCAGGT AGCAGATCAT GGGCACATCA TGTATAATGT GGACAGCAGC
    CCGCACCTTC CAGCATCCTT CAACTTGAAA GCAGACGATA AGCCAAAACT AGAGTTTTCC
    TTGCAAAGAA CTGCATCTCC TGGTCTGCGG CAGCCCGACA CTCCTCTGGA GAGAAGAGAT
    CCCAGCAGCC CAGTCCAAAC ACCCTCCTCT CCCCCACACA AACCTGAGCT TCCCCCCTCC
    GACTCAGAGA CGGCCTCAAG TGTAGCTACA GCCCCGACTC CTTCCATACC TGCCTCAACA
    GAAGAGTCAG CTGATGCCCC GAGCCCATTA GCTGAGCCCA GTCTCACTAA AGCTATCACA
    CCTGAACCAG AGAGCTCTGA GCCAGAGAAA TCCTCCTCAC CACCACCTCA AAGTCTGTCT
    GGATCCCTTA CCCAGCACGA AAAAGCCGTC AATGGGCTCA CGGATGTTGA CGCTGCTCCA
    CTGAGTGAAG AATTGGAAAC CCAACCCAGG GAAGCCTCTC CATTGCTGCC TACTTCCAGT
    GTGCCCCAGT CAGAGCCCAG GCCAGTCACA CCGGTGCTGG AGGAAGAGTC TGATCCAATC
    AATATGAACA GTCCTTTGCC TCCTGTTGAG GACGATGCTG GATGTCCTGA CAACGTTTCC
    CCCTCCCTCT CCACCTCCAC GACTGCTGCT ATCTCCACCA CTCCTCCTGC TCCTCCTCCA
    GGACTCTCGC ACCCCAGTCA GGTTTCTGCA GCGTTGGACA GGAGACCCTC TAATGGAGCA
    GAGATCAAAG AAACAGGGAA GGAAAACGAG GCCTTGCCGG ACAAGAGAGG AGAGCCTTTC
    TTACAGTCCA GAAAAAGCTC AAATCAAGCT ACATCTAGTG CACCAAAGAC CTGGAAGAAG
    CCAAAAGAGG ACATGCCTGT AGGGCAAGAT CAGTGTCCAG ACAAAGAGGA GGACACTGAT
    CAGGCCAGTA TGGAACCTGC TCCTGAAAGC CCCAGTCCTC CTCCTGTGGA GGTGGAGAGG
    AGGCCATCAG TAGGAGCAGT GCTCGAGGAG AATGGAGAAC AGGAAGTGGA GCCTTTGAGA
    AACGGTGCTG AACACACTTC TGAAACTGAG AGCAGTGACA GCATCCACCC GCCTGCAGTC
    AAAGAGCCGT TGATCAAAGT ATTGCCACAG CCTGCAGAGA AGAATGGAAA GAAGCAGTAC
    AACAGAGACT TCCTGCTGGG CTTCCAGTTT ATGCCTGCAT GTGTGCAGAA ACCAGAAGGG
    CTTCCTCCCA TTTCTGATGT TGTTCTAGAT AAGATTAACC AAAATAAGCT GCCCATGCGG
    CCGGTAGACC CACGTGTGCT TTCCAGAGGC CCGGACTTTA CGCCAGCGTT TGCGGATTTC
    AGCCGACCCA TTTCAGGAGG CAGAGGGGCA CCGTTGCTGA ATGTTGGCAT GCGACGGCCA
    CCTGGACGCA AGATCATCAC AAACGTGTCT GTGATTGATG AGGTGCAGCT GAAGACAACA
    GAGAATGCCT GGAAGCCTGG CATGAAGCGG GAAGGTGCAG TTGAAGATCC AGATGCCCAG
    AGAACTCAGG AGCTTTTCCG AAAAGTGCGC AGTATTCTCA ATAAACTGAC ACCGCAGATG
    TTCAGCCAGC TGATGAAGCA GGTGACTGAC CTCACCATCG ACACAGAGGA GCGACTCAAG
    GGTGTCATAG ATCTGGTCTT TGAAAAAGCA ATCAATGAGC CCAGCTTCTC TGTGGCCTAC
    GGCAACATGT GCAGCTGTCT GGCCACGTTA AAAGTGCCCA TGACGGATAA GCCCAACAGC
    ACTGTGAATT TCCGGAAGCT GCTTCTGAAC CGCTGTCAGA AAGAGTTTGA GAAGGACAAG
    ATGGATGACG ATGCCTTTGA GAAGAAACAC AGGGAGCTGG AGGCAGCTAC TGCGTCCAGT
    GAGCGGGAGA GGCTTCAGGA GGAGCTGGAA GAGGCCAAAG ACAAGGCCCG AAGACGCTCC
    ATTGGCAACA TTAAGTTCAT CGGCGAGCTC TTCAAGCTGC GGATGCTGAC AGAGGCCATC
    ATGCATGACT GCGTGGTGAA GTTGCTGAAA AACCATGATG AGGAATCACT GGAGTGCCTG
    TGTAGACTGC TCACCACTAT TGGAAAGGAC CTGGACTTTG AGAAAGCTAA GCCACGAATG
    GATCAGTACT TTAATCAGAT GGAGAAGATA GTTAAGGAGA GGAAGACTTC TTCACGCATT
    CGCTTCATGC TGCAGGATGT TATTGATCTG CGATTGCACA ACTGGGTTTC TCGAAGAGCA
    GATCAGGGCC CCAAGACCAT CGAGCAGATC CATAAAGACG CAAAGCTGGA GGAGCAGGAG
    GAACAGAGGA AAGTCCACCA GCAACTGCTT TCCAAAGACA ACAAGAGGAG ACCAGTGGTG
    CAGAGAGAGG AGACCTGGAG CACTGTGCCT ATGACTAAGA ACAGCAGAAC AATAGACCCC
    GCCAAGATTC CCAAATTCTC CAAGCCAGCG ATAGATGAGA AGATTCAGCT GGGGCCGAGA
    GCTCAGGTGA ACTGGATGAA GGGCAGCAGT GGTGGAGCTG GAGCAAAGGC CAGTGAGTCA
    GATGCGTCTC GGCCGAGTGC CAGTTTGAAT CGTTACTCTC CTCTGCAACC CTCGGCACTC
    CAGACTTCTT CTTTACCCTC CACATCTCCA GATTTTGATT CCAGGAGAGT TCTCGGCAGT
    CGTGGAAGCT CAGGACGGGA GCGCAATGAC AAGCCATTGA GTGCGGGTCC TGCTCGGACA
    GGGCCTATTT CATTGAGCAG TAGCAATAAG GAGACCCCCG AAGAGCTGGT TCAGGAAGTG
    TCCCGCAGAG ACTCCAATGC TTCAGATACA CCCAAGCTGC TCGTCAGTAC TGCAGACAAG
    AGCAGACTGG AGAACAGCCA ACCCAGAGAG TCTGCTGTTA AACTTGAAGC ACTCTCAGGG
    CCCTCTCCAG ATAAACCTGC ATTATCAGAA GAGGAGATGG AGAGGCGGTC CAAGTCTATA
    ATTGATGAGT TTTTGCACAT TAATGATTAC AAGGAAGCTT TGCAGTGTGT TGAGGAACTA
    GAGCAGTCTG CCATGCTCTA TGTGTTTGTG CGGGTGGGTG TGGAGTCCAC GCTGGAAAGG
    AGTCAGATCA CGCGAGACCA TATGGGTCAG CTGCTCTTCC AGCTTCTGCA GGCTGGAGTC
    CTCCCTAAAC TTCAGTTCTT TAAAGGGTTT TCTGAAACTC TTGAATTAGC AGATGATATG
    GCCATTGATA TTCCCCATAT ATGGCTGTAC CTTGCGGAGC TGGTAACCCC TGTGCTTCGA
    GAGGGGGGAA TCTCTATGAG AGAATTATTT AGTGAATTTA GCAAACCATT ACTCCCTGTG
    GGAAGAGCTG GGATATTATT TTCTGAAATA TTGCACCTTC TATGCAAACA AATGAGCCAT
    AAGAAAGTGG GATCATTATG GAGGGATTCT GGGTTGAGCT GGGCAGATTT TATACCTGAA
    ACAGAGGATG TGCACAGCTT CATCACAGAA CATAAACTAG AGTTCACTCT GTCTGATGGC
    TCAAACCCGA CCGAGGCTGT CTCTAAGAGA ACACTCTCGC CTGAGGAACT GAACAAGCAA
    CTTGAAAAAC TTTTACTTGA GGACATGGTT GGCGATGAAC AAATCTTCGA CTGGGTTGAG
    GCCAATCTAG ATGAATCAGA AATGAGTTCA GCTCCTTTCG TCAGAGCTTT AATGACTGCT
    GTGTGTAAAG CAGCAGTAAA AACTGAGGGC TCCTCGTGTA AAGTGGACTT GTCCATAATT
    CAGACGAGAT TGCCTGTATT ACACAAGTAC CTTAACTCAG ACACAGAGAG ACAGTTGCAA
    GCACTTTATG CACTTCAAGC ATTGATAGTA AAACTGGACC AGCCTGCTAA TTTGCTCCGG
    ATGTTTTTTG ACTGTTTGTA TGATGAGGAC GTGATCTCTG AAGACGCTTT CTATAAATGG
    GAGGTCAGTA AAGACCCCGC TGAACAGCAA GGCAAAGGTG TTGCCCTGAA GTCCGTCACA
    GCCTTCTTCA CGTGGCTGCG CGAGGCCGAG GAAGAGTCCG AGGACAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45534
    Clone ID Related Accession (Same CDS sequence) XM_009296898.2
    Accession Version XM_009296898.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296898.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACTAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CAACAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTGCCCCT GGGCCTCGTC CTACCCATCA CCAGGGAGGA
    TTCAGATCCA TTCAGTTTTT CCAGAGGACG CAGATGCAAA CGGCCCGGCC CACCATTCCA
    AGCAACACCC CACCCATTCG GCCCACGTCT CAGACCCCCA CGGCAGCAGT ATATTCTCCC
    AATCAGCACA TCATGATGAC CATGGCTCAC ATGCCCTTTC ATTCCCCACA GACAGCGCAG
    TACTACATCC CTCAGTATCG TCACAGTGCT CCACAGTATG TGGGACCTCC TCAACAGTAC
    CCTGTCCAGC CGACTGGACC CAGTACCTTC TATGCTGCAG CCAGTCCAGG GGAGTTTCCT
    GCACCTTATG CAGGACCTCC CTACTATCCT GGGCAGCCCG TGTACACACC ATCTCCACCC
    ATTATAGTGC CTACACCACA ACAACCTCCA CCTGCTAAGA GGGAAAAGAA AACAATCCGC
    ATCCGAGACC CTAACCAGGG TGGCAAGGAT GTCACAGACG AAATATTATC AGGAGTGGGT
    TTGAGTCGAA ACCCAACCCC GCCTGTGGGA CGGCCCTCTT CCACTCCTAC ACCTCCACAG
    CAGCTGAACA GCCAGGTAGC AGATCATGGG CACATCATGT ATAATGTGGA CAGCAGCCCG
    CACCTTCCAG CATCCTTCAA CTTGAAAGCA GACGATAAGC CAAAACTAGA GTTTTCCTTG
    CAAAGAACTG CATCTCCTGG TCTGCGGCAG CCCGACACTC CTCTGGAGAG AAGAGATCCC
    AGCAGCCCAG TCCAAACACC CTCCTCTCCC CCACACAAAC CTGAGCTTCC CCCCTCCGAC
    TCAGAGACGG CCTCAAGTGT AGCTACAGCC CCGACTCCTT CCATACCTGC CTCAACAGAA
    GAGTCAGCTG ATGCCCCGAG CCCATTAGCT GAGCCCAGTC TCACTAAAGC TATCACACCT
    GAACCAGAGA GCTCTGAGCC AGAGAAATCC TCCTCACCAC CACCTCAAAG TCTGTCTGGA
    TCCCTTACCC AGCACGAAAA AGCCGTCAAT GGGCTCACGG ATGTTGACGC TGCTCCACTG
    AGTGAAGAAT TGGAAACCCA ACCCAGGGAA GCCTCTCCAT TGCTGCCTAC TTCCAGTGTG
    CCCCAGTCAG AGCCCAGGCC AGTCACACCG GTGCTGGAGG AAGAGTCTGA TCCAATCAAT
    ATGAACAGTC CTTTGCCTCC TGTTGAGGAC GATGCTGGAT GTCCTGACAA CGTTTCCCCC
    TCCCTCTCCA CCTCCACGAC TGCTGCTATC TCCACCACTC CTCCTGCTCC TCCTCCAGGA
    CTCTCGCACC CCAGTCAGGT TTCTGCAGCG TTGGACAGGA GACCCTCTAA TGGAGCAGAG
    ATCAAAGAAA CAGGGAAGGA AAACGAGGCC TTGCCGGACA AGAGAGGAGA GCCTTTCTTA
    CAGTCCAGAA AAAGCTCAAA TCAAGCTACA TCTAGTGCAC CAAAGACCTG GAAGAAGCCA
    AAAGAGGACA TGCCTGTAGG GCAAGATCAG TGTCCAGACA AAGAGGAGGA CACTGATCAG
    GCCAGTATGG AACCTGCTCC TGAAAGCCCC AGTCCTCCTC CTGTGGAGGT GGAGAGGAGG
    CCATCAGTAG GAGCAGTGCT CGAGGAGAAT GGAGAACAGG AAGTGGAGCC TTTGAGAAAC
    GGTGCTGAAC ACACTTCTGA AACTGAGAGC AGTGACAGCA TCCACCCGCC TGCAGTCAAA
    GAGCCGTTGA TCAAAGTATT GCCACAGCCT GCAGAGAAGA ATGGAAAGAA GCAGTACAAC
    AGAGACTTCC TGCTGGGCTT CCAGTTTATG CCTGCATGTG TGCAGAAACC AGAAGGGCTT
    CCTCCCATTT CTGATGTTGT TCTAGATAAG ATTAACCAAA ATAAGCTGCC CATGCGGCCG
    GTAGACCCAC GTGTGCTTTC CAGAGGCCCG GACTTTACGC CAGCGTTTGC GGATTTCAGC
    CGACCCATTT CAGGAGGCAG AGGGGCACCG TTGCTGAATG TTGGCATGCG ACGGCCACCT
    GGACGCAAGA TCATCACAAA CGTGTCTGTG ATTGATGAGG TGCAGCTGAA GACAACAGAG
    AATGCCTGGA AGCCTGGCAT GAAGCGGGAA GGTGCAGTTG AAGATCCAGA TGCCCAGAGA
    ACTCAGGAGC TTTTCCGAAA AGTGCGCAGT ATTCTCAATA AACTGACACC GCAGATGTTC
    AGCCAGCTGA TGAAGCAGGT GACTGACCTC ACCATCGACA CAGAGGAGCG ACTCAAGGGT
    GTCATAGATC TGGTCTTTGA AAAAGCAATC AATGAGCCCA GCTTCTCTGT GGCCTACGGC
    AACATGTGCA GCTGTCTGGC CACGTTAAAA GTGCCCATGA CGGATAAGCC CAACAGCACT
    GTGAATTTCC GGAAGCTGCT TCTGAACCGC TGTCAGAAAG AGTTTGAGAA GGACAAGATG
    GATGACGATG CCTTTGAGAA GAAACACAGG GAGCTGGAGG CAGCTACTGC GTCCAGTGAG
    CGGGAGAGGC TTCAGGAGGA GCTGGAAGAG GCCAAAGACA AGGCCCGAAG ACGCTCCATT
    GGCAACATTA AGTTCATCGG CGAGCTCTTC AAGCTGCGGA TGCTGACAGA GGCCATCATG
    CATGACTGCG TGGTGAAGTT GCTGAAAAAC CATGATGAGG AATCACTGGA GTGCCTGTGT
    AGACTGCTCA CCACTATTGG AAAGGACCTG GACTTTGAGA AAGCTAAGCC ACGAATGGAT
    CAGTACTTTA ATCAGATGGA GAAGATAGTT AAGGAGAGGA AGACTTCTTC ACGCATTCGC
    TTCATGCTGC AGGATGTTAT TGATCTGCGA TTGCACAACT GGGTTTCTCG AAGAGCAGAT
    CAGGGCCCCA AGACCATCGA GCAGATCCAT AAAGACGCAA AGCTGGAGGA GCAGGAGGAA
    CAGAGGAAAG TCCACCAGCA ACTGCTTTCC AAAGACAACA AGAGGAGACC AGTGGTGCAG
    AGAGAGGAGA CCTGGAGCAC TGTGCCTATG ACTAAGAACA GCAGAACAAT AGACCCCGCC
    AAGATTCCCA AATTCTCCAA GCCAGCGATA GATGAGAAGA TTCAGCTGGG GCCGAGAGCT
    CAGGTGAACT GGATGAAGGG CAGCAGTGGT GGAGCTGGAG CAAAGGCCAG TGAGTCAGAT
    GCGTCTCGGC CGAGTGCCAG TTTGAATCGT TACTCTCCTC TGCAACCCTC GGCACTCCAG
    ACTTCTTCTT TACCCTCCAC ATCTCCAGAT TTTGATTCCA GGAGAGTTCT CGGCAGTCGT
    GGAAGCTCAG GACGGGAGCG CAATGACAAG CCATTGAGTG CGGGTCCTGC TCGGACAGGG
    CCTATTTCAT TGAGCAGTAG CAATAAGGAG ACCCCCGAAG AGCTGGTTCA GGAAGTGTCC
    CGCAGAGACT CCAATGCTTC AGATACACCC AAGCTGCTCG TCAGTACTGC AGACAAGAGC
    AGACTGGAGA ACAGCCAACC CAGAGAGTCT GCTGTTAAAC TTGAAGCACT CTCAGGGCCC
    TCTCCAGATA AACCTGCATT ATCAGAAGAG GAGATGGAGA GGCGGTCCAA GTCTATAATT
    GATGAGTTTT TGCACATTAA TGATTACAAG GAAGCTTTGC AGTGTGTTGA GGAACTAGAG
    CAGTCTGCCA TGCTCTATGT GTTTGTGCGG GTGGGTGTGG AGTCCACGCT GGAAAGGAGT
    CAGATCACGC GAGACCATAT GGGTCAGCTG CTCTTCCAGC TTCTGCAGGC TGGAGTCCTC
    CCTAAACTTC AGTTCTTTAA AGGGTTTTCT GAAACTCTTG AATTAGCAGA TGATATGGCC
    ATTGATATTC CCCATATATG GCTGTACCTT GCGGAGCTGG TAACCCCTGT GCTTCGAGAG
    GGGGGAATCT CTATGAGAGA ATTATTTAGT GAATTTAGCA AACCATTACT CCCTGTGGGA
    AGAGCTGGGA TATTATTTTC TGAAATATTG CACCTTCTAT GCAAACAAAT GAGCCATAAG
    AAAGTGGGAT CATTATGGAG GGATTCTGGG TTGAGCTGGG CAGATTTTAT ACCTGAAACA
    GAGGATGTGC ACAGCTTCAT CACAGAACAT AAACTAGAGT TCACTCTGTC TGATGGCTCA
    AACCCGACCG AGGCTGTCTC TAAGAGAACA CTCTCGCCTG AGGAACTGAA CAAGCAACTT
    GAAAAACTTT TACTTGAGGA CATGGTTGGC GATGAACAAA TCTTCGACTG GGTTGAGGCC
    AATCTAGATG AATCAGAAAT GAGTTCAGCT CCTTTCGTCA GAGCTTTAAT GACTGCTGTG
    TGTAAAGCAG CAGTAAAAAC TGAGGGCTCC TCGTGTAAAG TGGACTTGTC CATAATTCAG
    ACGAGATTGC CTGTATTACA CAAGTACCTT AACTCAGACA CAGAGAGACA GTTGCAAGCA
    CTTTATGCAC TTCAAGCATT GATAGTAAAA CTGGACCAGC CTGCTAATTT GCTCCGGATG
    TTTTTTGACT GTTTGTATGA TGAGGACGTG ATCTCTGAAG ACGCTTTCTA TAAATGGGAG
    GTCAGTAAAG ACCCCGCTGA ACAGCAAGGC AAAGGTGTTG CCCTGAAGTC CGTCACAGCC
    TTCTTCACGT GGCTGCGCGA GGCCGAGGAA GAGTCCGAGG ACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295173.1
    CDS606..5753
    Translation

    Target ORF information:

    RefSeq Version XM_009296898.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296898.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACTAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CAACAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTGCCCCT GGGCCTCGTC CTACCCATCA CCAGGGAGGA
    TTCAGATCCA TTCAGTTTTT CCAGAGGACG CAGATGCAAA CGGCCCGGCC CACCATTCCA
    AGCAACACCC CACCCATTCG GCCCACGTCT CAGACCCCCA CGGCAGCAGT ATATTCTCCC
    AATCAGCACA TCATGATGAC CATGGCTCAC ATGCCCTTTC ATTCCCCACA GACAGCGCAG
    TACTACATCC CTCAGTATCG TCACAGTGCT CCACAGTATG TGGGACCTCC TCAACAGTAC
    CCTGTCCAGC CGACTGGACC CAGTACCTTC TATGCTGCAG CCAGTCCAGG GGAGTTTCCT
    GCACCTTATG CAGGACCTCC CTACTATCCT GGGCAGCCCG TGTACACACC ATCTCCACCC
    ATTATAGTGC CTACACCACA ACAACCTCCA CCTGCTAAGA GGGAAAAGAA AACAATCCGC
    ATCCGAGACC CTAACCAGGG TGGCAAGGAT GTCACAGACG AAATATTATC AGGAGTGGGT
    TTGAGTCGAA ACCCAACCCC GCCTGTGGGA CGGCCCTCTT CCACTCCTAC ACCTCCACAG
    CAGCTGAACA GCCAGGTAGC AGATCATGGG CACATCATGT ATAATGTGGA CAGCAGCCCG
    CACCTTCCAG CATCCTTCAA CTTGAAAGCA GACGATAAGC CAAAACTAGA GTTTTCCTTG
    CAAAGAACTG CATCTCCTGG TCTGCGGCAG CCCGACACTC CTCTGGAGAG AAGAGATCCC
    AGCAGCCCAG TCCAAACACC CTCCTCTCCC CCACACAAAC CTGAGCTTCC CCCCTCCGAC
    TCAGAGACGG CCTCAAGTGT AGCTACAGCC CCGACTCCTT CCATACCTGC CTCAACAGAA
    GAGTCAGCTG ATGCCCCGAG CCCATTAGCT GAGCCCAGTC TCACTAAAGC TATCACACCT
    GAACCAGAGA GCTCTGAGCC AGAGAAATCC TCCTCACCAC CACCTCAAAG TCTGTCTGGA
    TCCCTTACCC AGCACGAAAA AGCCGTCAAT GGGCTCACGG ATGTTGACGC TGCTCCACTG
    AGTGAAGAAT TGGAAACCCA ACCCAGGGAA GCCTCTCCAT TGCTGCCTAC TTCCAGTGTG
    CCCCAGTCAG AGCCCAGGCC AGTCACACCG GTGCTGGAGG AAGAGTCTGA TCCAATCAAT
    ATGAACAGTC CTTTGCCTCC TGTTGAGGAC GATGCTGGAT GTCCTGACAA CGTTTCCCCC
    TCCCTCTCCA CCTCCACGAC TGCTGCTATC TCCACCACTC CTCCTGCTCC TCCTCCAGGA
    CTCTCGCACC CCAGTCAGGT TTCTGCAGCG TTGGACAGGA GACCCTCTAA TGGAGCAGAG
    ATCAAAGAAA CAGGGAAGGA AAACGAGGCC TTGCCGGACA AGAGAGGAGA GCCTTTCTTA
    CAGTCCAGAA AAAGCTCAAA TCAAGCTACA TCTAGTGCAC CAAAGACCTG GAAGAAGCCA
    AAAGAGGACA TGCCTGTAGG GCAAGATCAG TGTCCAGACA AAGAGGAGGA CACTGATCAG
    GCCAGTATGG AACCTGCTCC TGAAAGCCCC AGTCCTCCTC CTGTGGAGGT GGAGAGGAGG
    CCATCAGTAG GAGCAGTGCT CGAGGAGAAT GGAGAACAGG AAGTGGAGCC TTTGAGAAAC
    GGTGCTGAAC ACACTTCTGA AACTGAGAGC AGTGACAGCA TCCACCCGCC TGCAGTCAAA
    GAGCCGTTGA TCAAAGTATT GCCACAGCCT GCAGAGAAGA ATGGAAAGAA GCAGTACAAC
    AGAGACTTCC TGCTGGGCTT CCAGTTTATG CCTGCATGTG TGCAGAAACC AGAAGGGCTT
    CCTCCCATTT CTGATGTTGT TCTAGATAAG ATTAACCAAA ATAAGCTGCC CATGCGGCCG
    GTAGACCCAC GTGTGCTTTC CAGAGGCCCG GACTTTACGC CAGCGTTTGC GGATTTCAGC
    CGACCCATTT CAGGAGGCAG AGGGGCACCG TTGCTGAATG TTGGCATGCG ACGGCCACCT
    GGACGCAAGA TCATCACAAA CGTGTCTGTG ATTGATGAGG TGCAGCTGAA GACAACAGAG
    AATGCCTGGA AGCCTGGCAT GAAGCGGGAA GGTGCAGTTG AAGATCCAGA TGCCCAGAGA
    ACTCAGGAGC TTTTCCGAAA AGTGCGCAGT ATTCTCAATA AACTGACACC GCAGATGTTC
    AGCCAGCTGA TGAAGCAGGT GACTGACCTC ACCATCGACA CAGAGGAGCG ACTCAAGGGT
    GTCATAGATC TGGTCTTTGA AAAAGCAATC AATGAGCCCA GCTTCTCTGT GGCCTACGGC
    AACATGTGCA GCTGTCTGGC CACGTTAAAA GTGCCCATGA CGGATAAGCC CAACAGCACT
    GTGAATTTCC GGAAGCTGCT TCTGAACCGC TGTCAGAAAG AGTTTGAGAA GGACAAGATG
    GATGACGATG CCTTTGAGAA GAAACACAGG GAGCTGGAGG CAGCTACTGC GTCCAGTGAG
    CGGGAGAGGC TTCAGGAGGA GCTGGAAGAG GCCAAAGACA AGGCCCGAAG ACGCTCCATT
    GGCAACATTA AGTTCATCGG CGAGCTCTTC AAGCTGCGGA TGCTGACAGA GGCCATCATG
    CATGACTGCG TGGTGAAGTT GCTGAAAAAC CATGATGAGG AATCACTGGA GTGCCTGTGT
    AGACTGCTCA CCACTATTGG AAAGGACCTG GACTTTGAGA AAGCTAAGCC ACGAATGGAT
    CAGTACTTTA ATCAGATGGA GAAGATAGTT AAGGAGAGGA AGACTTCTTC ACGCATTCGC
    TTCATGCTGC AGGATGTTAT TGATCTGCGA TTGCACAACT GGGTTTCTCG AAGAGCAGAT
    CAGGGCCCCA AGACCATCGA GCAGATCCAT AAAGACGCAA AGCTGGAGGA GCAGGAGGAA
    CAGAGGAAAG TCCACCAGCA ACTGCTTTCC AAAGACAACA AGAGGAGACC AGTGGTGCAG
    AGAGAGGAGA CCTGGAGCAC TGTGCCTATG ACTAAGAACA GCAGAACAAT AGACCCCGCC
    AAGATTCCCA AATTCTCCAA GCCAGCGATA GATGAGAAGA TTCAGCTGGG GCCGAGAGCT
    CAGGTGAACT GGATGAAGGG CAGCAGTGGT GGAGCTGGAG CAAAGGCCAG TGAGTCAGAT
    GCGTCTCGGC CGAGTGCCAG TTTGAATCGT TACTCTCCTC TGCAACCCTC GGCACTCCAG
    ACTTCTTCTT TACCCTCCAC ATCTCCAGAT TTTGATTCCA GGAGAGTTCT CGGCAGTCGT
    GGAAGCTCAG GACGGGAGCG CAATGACAAG CCATTGAGTG CGGGTCCTGC TCGGACAGGG
    CCTATTTCAT TGAGCAGTAG CAATAAGGAG ACCCCCGAAG AGCTGGTTCA GGAAGTGTCC
    CGCAGAGACT CCAATGCTTC AGATACACCC AAGCTGCTCG TCAGTACTGC AGACAAGAGC
    AGACTGGAGA ACAGCCAACC CAGAGAGTCT GCTGTTAAAC TTGAAGCACT CTCAGGGCCC
    TCTCCAGATA AACCTGCATT ATCAGAAGAG GAGATGGAGA GGCGGTCCAA GTCTATAATT
    GATGAGTTTT TGCACATTAA TGATTACAAG GAAGCTTTGC AGTGTGTTGA GGAACTAGAG
    CAGTCTGCCA TGCTCTATGT GTTTGTGCGG GTGGGTGTGG AGTCCACGCT GGAAAGGAGT
    CAGATCACGC GAGACCATAT GGGTCAGCTG CTCTTCCAGC TTCTGCAGGC TGGAGTCCTC
    CCTAAACTTC AGTTCTTTAA AGGGTTTTCT GAAACTCTTG AATTAGCAGA TGATATGGCC
    ATTGATATTC CCCATATATG GCTGTACCTT GCGGAGCTGG TAACCCCTGT GCTTCGAGAG
    GGGGGAATCT CTATGAGAGA ATTATTTAGT GAATTTAGCA AACCATTACT CCCTGTGGGA
    AGAGCTGGGA TATTATTTTC TGAAATATTG CACCTTCTAT GCAAACAAAT GAGCCATAAG
    AAAGTGGGAT CATTATGGAG GGATTCTGGG TTGAGCTGGG CAGATTTTAT ACCTGAAACA
    GAGGATGTGC ACAGCTTCAT CACAGAACAT AAACTAGAGT TCACTCTGTC TGATGGCTCA
    AACCCGACCG AGGCTGTCTC TAAGAGAACA CTCTCGCCTG AGGAACTGAA CAAGCAACTT
    GAAAAACTTT TACTTGAGGA CATGGTTGGC GATGAACAAA TCTTCGACTG GGTTGAGGCC
    AATCTAGATG AATCAGAAAT GAGTTCAGCT CCTTTCGTCA GAGCTTTAAT GACTGCTGTG
    TGTAAAGCAG CAGTAAAAAC TGAGGGCTCC TCGTGTAAAG TGGACTTGTC CATAATTCAG
    ACGAGATTGC CTGTATTACA CAAGTACCTT AACTCAGACA CAGAGAGACA GTTGCAAGCA
    CTTTATGCAC TTCAAGCATT GATAGTAAAA CTGGACCAGC CTGCTAATTT GCTCCGGATG
    TTTTTTGACT GTTTGTATGA TGAGGACGTG ATCTCTGAAG ACGCTTTCTA TAAATGGGAG
    GTCAGTAAAG ACCCCGCTGA ACAGCAAGGC AAAGGTGTTG CCCTGAAGTC CGTCACAGCC
    TTCTTCACGT GGCTGCGCGA GGCCGAGGAA GAGTCCGAGG ACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45535
    Clone ID Related Accession (Same CDS sequence) XM_009296899.2 , XM_009296899.3
    Accession Version XM_009296899.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296899.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACTAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CAACAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTGCCCCT GGGCCTCGTC CTACCCATCA CCAGGGAGGA
    TTCAGATCCA TTCAGTTTTT CCAGAGGACG CAGATGCAAA CGGCCCGGCC CACCATTCCA
    AGCAACACCC CACCCATTCG GCCCACGTCT CAGACCCCCA CGGCAGCAGT ATATTCTCCC
    AATCAGCACA TCATGATGAC CATGGCTCAC ATGCCCTTTC ATTCCCCACA GACAGCGCAG
    TACTACATCC CTCAGTATCG TCACAGTGCT CCACAGTATG TGGGACCTCC TCAACAGTAC
    CCTGTCCAGC CGACTGGACC CAGTACCTTC TATGCTGCAG CCAGTCCAGG GGAGTTTCCT
    GCACCTTATG CAGCAGGACC TCCCTACTAT CCTGGGCAGC CCGTGTACAC ACCATCTCCA
    CCCATTATAG TGCCTACACC ACAACAACCT CCACCTGCTA AGAGGGAAAA GAAAACAATC
    CGCATCCGAG ACCCTAACCA GGGTGGCAAG GATGTCACAG ACGAAATATT ATCAGGAGTG
    GGTTTGAGTC GAAACCCAAC CCCGCCTGTG GGACGGCCCT CTTCCACTCC TACACCTCCA
    CAGCTGAACA GCCAGGTAGC AGATCATGGG CACATCATGT ATAATGTGGA CAGCAGCCCG
    CACCTTCCAG CATCCTTCAA CTTGAAAGCA GACGATAAGC CAAAACTAGA GTTTTCCTTG
    CAAAGAACTG CATCTCCTGG TCTGCGGCAG CCCGACACTC CTCTGGAGAG AAGAGATCCC
    AGCAGCCCAG TCCAAACACC CTCCTCTCCC CCACACAAAC CTGAGCTTCC CCCCTCCGAC
    TCAGAGACGG CCTCAAGTGT AGCTACAGCC CCGACTCCTT CCATACCTGC CTCAACAGAA
    GAGTCAGCTG ATGCCCCGAG CCCATTAGCT GAGCCCAGTC TCACTAAAGC TATCACACCT
    GAACCAGAGA GCTCTGAGCC AGAGAAATCC TCCTCACCAC CACCTCAAAG TCTGTCTGGA
    TCCCTTACCC AGCACGAAAA AGCCGTCAAT GGGCTCACGG ATGTTGACGC TGCTCCACTG
    AGTGAAGAAT TGGAAACCCA ACCCAGGGAA GCCTCTCCAT TGCTGCCTAC TTCCAGTGTG
    CCCCAGTCAG AGCCCAGGCC AGTCACACCG GTGCTGGAGG AAGAGTCTGA TCCAATCAAT
    ATGAACAGTC CTTTGCCTCC TGTTGAGGAC GATGCTGGAT GTCCTGACAA CGTTTCCCCC
    TCCCTCTCCA CCTCCACGAC TGCTGCTATC TCCACCACTC CTCCTGCTCC TCCTCCAGGA
    CTCTCGCACC CCAGTCAGGT TTCTGCAGCG TTGGACAGGA GACCCTCTAA TGGAGCAGAG
    ATCAAAGAAA CAGGGAAGGA AAACGAGGCC TTGCCGGACA AGAGAGGAGA GCCTTTCTTA
    CAGTCCAGAA AAAGCTCAAA TCAAGCTACA TCTAGTGCAC CAAAGACCTG GAAGAAGCCA
    AAAGAGGACA TGCCTGTAGG GCAAGATCAG TGTCCAGACA AAGAGGAGGA CACTGATCAG
    GCCAGTATGG AACCTGCTCC TGAAAGCCCC AGTCCTCCTC CTGTGGAGGT GGAGAGGAGG
    CCATCAGTAG GAGCAGTGCT CGAGGAGAAT GGAGAACAGG AAGTGGAGCC TTTGAGAAAC
    GGTGCTGAAC ACACTTCTGA AACTGAGAGC AGTGACAGCA TCCACCCGCC TGCAGTCAAA
    GAGCCGTTGA TCAAAGTATT GCCACAGCCT GCAGAGAAGA ATGGAAAGAA GCAGTACAAC
    AGAGACTTCC TGCTGGGCTT CCAGTTTATG CCTGCATGTG TGCAGAAACC AGAAGGGCTT
    CCTCCCATTT CTGATGTTGT TCTAGATAAG ATTAACCAAA ATAAGCTGCC CATGCGGCCG
    GTAGACCCAC GTGTGCTTTC CAGAGGCCCG GACTTTACGC CAGCGTTTGC GGATTTCAGC
    CGACCCATTT CAGGAGGCAG AGGGGCACCG TTGCTGAATG TTGGCATGCG ACGGCCACCT
    GGACGCAAGA TCATCACAAA CGTGTCTGTG ATTGATGAGG TGCAGCTGAA GACAACAGAG
    AATGCCTGGA AGCCTGGCAT GAAGCGGGAA GGTGCAGTTG AAGATCCAGA TGCCCAGAGA
    ACTCAGGAGC TTTTCCGAAA AGTGCGCAGT ATTCTCAATA AACTGACACC GCAGATGTTC
    AGCCAGCTGA TGAAGCAGGT GACTGACCTC ACCATCGACA CAGAGGAGCG ACTCAAGGGT
    GTCATAGATC TGGTCTTTGA AAAAGCAATC AATGAGCCCA GCTTCTCTGT GGCCTACGGC
    AACATGTGCA GCTGTCTGGC CACGTTAAAA GTGCCCATGA CGGATAAGCC CAACAGCACT
    GTGAATTTCC GGAAGCTGCT TCTGAACCGC TGTCAGAAAG AGTTTGAGAA GGACAAGATG
    GATGACGATG CCTTTGAGAA GAAACACAGG GAGCTGGAGG CAGCTACTGC GTCCAGTGAG
    CGGGAGAGGC TTCAGGAGGA GCTGGAAGAG GCCAAAGACA AGGCCCGAAG ACGCTCCATT
    GGCAACATTA AGTTCATCGG CGAGCTCTTC AAGCTGCGGA TGCTGACAGA GGCCATCATG
    CATGACTGCG TGGTGAAGTT GCTGAAAAAC CATGATGAGG AATCACTGGA GTGCCTGTGT
    AGACTGCTCA CCACTATTGG AAAGGACCTG GACTTTGAGA AAGCTAAGCC ACGAATGGAT
    CAGTACTTTA ATCAGATGGA GAAGATAGTT AAGGAGAGGA AGACTTCTTC ACGCATTCGC
    TTCATGCTGC AGGATGTTAT TGATCTGCGA TTGCACAACT GGGTTTCTCG AAGAGCAGAT
    CAGGGCCCCA AGACCATCGA GCAGATCCAT AAAGACGCAA AGCTGGAGGA GCAGGAGGAA
    CAGAGGAAAG TCCACCAGCA ACTGCTTTCC AAAGACAACA AGAGGAGACC AGTGGTGCAG
    AGAGAGGAGA CCTGGAGCAC TGTGCCTATG ACTAAGAACA GCAGAACAAT AGACCCCGCC
    AAGATTCCCA AATTCTCCAA GCCAGCGATA GATGAGAAGA TTCAGCTGGG GCCGAGAGCT
    CAGGTGAACT GGATGAAGGG CAGCAGTGGT GGAGCTGGAG CAAAGGCCAG TGAGTCAGAT
    GCGTCTCGGC CGAGTGCCAG TTTGAATCGT TACTCTCCTC TGCAACCCTC GGCACTCCAG
    ACTTCTTCTT TACCCTCCAC ATCTCCAGAT TTTGATTCCA GGAGAGTTCT CGGCAGTCGT
    GGAAGCTCAG GACGGGAGCG CAATGACAAG CCATTGAGTG CGGGTCCTGC TCGGACAGGG
    CCTATTTCAT TGAGCAGTAG CAATAAGGAG ACCCCCGAAG AGCTGGTTCA GGAAGTGTCC
    CGCAGAGACT CCAATGCTTC AGATACACCC AAGCTGCTCG TCAGTACTGC AGACAAGAGC
    AGACTGGAGA ACAGCCAACC CAGAGAGTCT GCTGTTAAAC TTGAAGCACT CTCAGGGCCC
    TCTCCAGATA AACCTGCATT ATCAGAAGAG GAGATGGAGA GGCGGTCCAA GTCTATAATT
    GATGAGTTTT TGCACATTAA TGATTACAAG GAAGCTTTGC AGTGTGTTGA GGAACTAGAG
    CAGTCTGCCA TGCTCTATGT GTTTGTGCGG GTGGGTGTGG AGTCCACGCT GGAAAGGAGT
    CAGATCACGC GAGACCATAT GGGTCAGCTG CTCTTCCAGC TTCTGCAGGC TGGAGTCCTC
    CCTAAACTTC AGTTCTTTAA AGGGTTTTCT GAAACTCTTG AATTAGCAGA TGATATGGCC
    ATTGATATTC CCCATATATG GCTGTACCTT GCGGAGCTGG TAACCCCTGT GCTTCGAGAG
    GGGGGAATCT CTATGAGAGA ATTATTTAGT GAATTTAGCA AACCATTACT CCCTGTGGGA
    AGAGCTGGGA TATTATTTTC TGAAATATTG CACCTTCTAT GCAAACAAAT GAGCCATAAG
    AAAGTGGGAT CATTATGGAG GGATTCTGGG TTGAGCTGGG CAGATTTTAT ACCTGAAACA
    GAGGATGTGC ACAGCTTCAT CACAGAACAT AAACTAGAGT TCACTCTGTC TGATGGCTCA
    AACCCGACCG AGGCTGTCTC TAAGAGAACA CTCTCGCCTG AGGAACTGAA CAAGCAACTT
    GAAAAACTTT TACTTGAGGA CATGGTTGGC GATGAACAAA TCTTCGACTG GGTTGAGGCC
    AATCTAGATG AATCAGAAAT GAGTTCAGCT CCTTTCGTCA GAGCTTTAAT GACTGCTGTG
    TGTAAAGCAG CAGTAAAAAC TGAGGGCTCC TCGTGTAAAG TGGACTTGTC CATAATTCAG
    ACGAGATTGC CTGTATTACA CAAGTACCTT AACTCAGACA CAGAGAGACA GTTGCAAGCA
    CTTTATGCAC TTCAAGCATT GATAGTAAAA CTGGACCAGC CTGCTAATTT GCTCCGGATG
    TTTTTTGACT GTTTGTATGA TGAGGACGTG ATCTCTGAAG ACGCTTTCTA TAAATGGGAG
    GTCAGTAAAG ACCCCGCTGA ACAGCAAGGC AAAGGTGTTG CCCTGAAGTC CGTCACAGCC
    TTCTTCACGT GGCTGCGCGA GGCCGAGGAA GAGTCCGAGG ACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295174.1
    CDS606..5753
    Translation

    Target ORF information:

    RefSeq Version XM_009296899.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296899.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACTAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CAACAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTGCCCCT GGGCCTCGTC CTACCCATCA CCAGGGAGGA
    TTCAGATCCA TTCAGTTTTT CCAGAGGACG CAGATGCAAA CGGCCCGGCC CACCATTCCA
    AGCAACACCC CACCCATTCG GCCCACGTCT CAGACCCCCA CGGCAGCAGT ATATTCTCCC
    AATCAGCACA TCATGATGAC CATGGCTCAC ATGCCCTTTC ATTCCCCACA GACAGCGCAG
    TACTACATCC CTCAGTATCG TCACAGTGCT CCACAGTATG TGGGACCTCC TCAACAGTAC
    CCTGTCCAGC CGACTGGACC CAGTACCTTC TATGCTGCAG CCAGTCCAGG GGAGTTTCCT
    GCACCTTATG CAGCAGGACC TCCCTACTAT CCTGGGCAGC CCGTGTACAC ACCATCTCCA
    CCCATTATAG TGCCTACACC ACAACAACCT CCACCTGCTA AGAGGGAAAA GAAAACAATC
    CGCATCCGAG ACCCTAACCA GGGTGGCAAG GATGTCACAG ACGAAATATT ATCAGGAGTG
    GGTTTGAGTC GAAACCCAAC CCCGCCTGTG GGACGGCCCT CTTCCACTCC TACACCTCCA
    CAGCTGAACA GCCAGGTAGC AGATCATGGG CACATCATGT ATAATGTGGA CAGCAGCCCG
    CACCTTCCAG CATCCTTCAA CTTGAAAGCA GACGATAAGC CAAAACTAGA GTTTTCCTTG
    CAAAGAACTG CATCTCCTGG TCTGCGGCAG CCCGACACTC CTCTGGAGAG AAGAGATCCC
    AGCAGCCCAG TCCAAACACC CTCCTCTCCC CCACACAAAC CTGAGCTTCC CCCCTCCGAC
    TCAGAGACGG CCTCAAGTGT AGCTACAGCC CCGACTCCTT CCATACCTGC CTCAACAGAA
    GAGTCAGCTG ATGCCCCGAG CCCATTAGCT GAGCCCAGTC TCACTAAAGC TATCACACCT
    GAACCAGAGA GCTCTGAGCC AGAGAAATCC TCCTCACCAC CACCTCAAAG TCTGTCTGGA
    TCCCTTACCC AGCACGAAAA AGCCGTCAAT GGGCTCACGG ATGTTGACGC TGCTCCACTG
    AGTGAAGAAT TGGAAACCCA ACCCAGGGAA GCCTCTCCAT TGCTGCCTAC TTCCAGTGTG
    CCCCAGTCAG AGCCCAGGCC AGTCACACCG GTGCTGGAGG AAGAGTCTGA TCCAATCAAT
    ATGAACAGTC CTTTGCCTCC TGTTGAGGAC GATGCTGGAT GTCCTGACAA CGTTTCCCCC
    TCCCTCTCCA CCTCCACGAC TGCTGCTATC TCCACCACTC CTCCTGCTCC TCCTCCAGGA
    CTCTCGCACC CCAGTCAGGT TTCTGCAGCG TTGGACAGGA GACCCTCTAA TGGAGCAGAG
    ATCAAAGAAA CAGGGAAGGA AAACGAGGCC TTGCCGGACA AGAGAGGAGA GCCTTTCTTA
    CAGTCCAGAA AAAGCTCAAA TCAAGCTACA TCTAGTGCAC CAAAGACCTG GAAGAAGCCA
    AAAGAGGACA TGCCTGTAGG GCAAGATCAG TGTCCAGACA AAGAGGAGGA CACTGATCAG
    GCCAGTATGG AACCTGCTCC TGAAAGCCCC AGTCCTCCTC CTGTGGAGGT GGAGAGGAGG
    CCATCAGTAG GAGCAGTGCT CGAGGAGAAT GGAGAACAGG AAGTGGAGCC TTTGAGAAAC
    GGTGCTGAAC ACACTTCTGA AACTGAGAGC AGTGACAGCA TCCACCCGCC TGCAGTCAAA
    GAGCCGTTGA TCAAAGTATT GCCACAGCCT GCAGAGAAGA ATGGAAAGAA GCAGTACAAC
    AGAGACTTCC TGCTGGGCTT CCAGTTTATG CCTGCATGTG TGCAGAAACC AGAAGGGCTT
    CCTCCCATTT CTGATGTTGT TCTAGATAAG ATTAACCAAA ATAAGCTGCC CATGCGGCCG
    GTAGACCCAC GTGTGCTTTC CAGAGGCCCG GACTTTACGC CAGCGTTTGC GGATTTCAGC
    CGACCCATTT CAGGAGGCAG AGGGGCACCG TTGCTGAATG TTGGCATGCG ACGGCCACCT
    GGACGCAAGA TCATCACAAA CGTGTCTGTG ATTGATGAGG TGCAGCTGAA GACAACAGAG
    AATGCCTGGA AGCCTGGCAT GAAGCGGGAA GGTGCAGTTG AAGATCCAGA TGCCCAGAGA
    ACTCAGGAGC TTTTCCGAAA AGTGCGCAGT ATTCTCAATA AACTGACACC GCAGATGTTC
    AGCCAGCTGA TGAAGCAGGT GACTGACCTC ACCATCGACA CAGAGGAGCG ACTCAAGGGT
    GTCATAGATC TGGTCTTTGA AAAAGCAATC AATGAGCCCA GCTTCTCTGT GGCCTACGGC
    AACATGTGCA GCTGTCTGGC CACGTTAAAA GTGCCCATGA CGGATAAGCC CAACAGCACT
    GTGAATTTCC GGAAGCTGCT TCTGAACCGC TGTCAGAAAG AGTTTGAGAA GGACAAGATG
    GATGACGATG CCTTTGAGAA GAAACACAGG GAGCTGGAGG CAGCTACTGC GTCCAGTGAG
    CGGGAGAGGC TTCAGGAGGA GCTGGAAGAG GCCAAAGACA AGGCCCGAAG ACGCTCCATT
    GGCAACATTA AGTTCATCGG CGAGCTCTTC AAGCTGCGGA TGCTGACAGA GGCCATCATG
    CATGACTGCG TGGTGAAGTT GCTGAAAAAC CATGATGAGG AATCACTGGA GTGCCTGTGT
    AGACTGCTCA CCACTATTGG AAAGGACCTG GACTTTGAGA AAGCTAAGCC ACGAATGGAT
    CAGTACTTTA ATCAGATGGA GAAGATAGTT AAGGAGAGGA AGACTTCTTC ACGCATTCGC
    TTCATGCTGC AGGATGTTAT TGATCTGCGA TTGCACAACT GGGTTTCTCG AAGAGCAGAT
    CAGGGCCCCA AGACCATCGA GCAGATCCAT AAAGACGCAA AGCTGGAGGA GCAGGAGGAA
    CAGAGGAAAG TCCACCAGCA ACTGCTTTCC AAAGACAACA AGAGGAGACC AGTGGTGCAG
    AGAGAGGAGA CCTGGAGCAC TGTGCCTATG ACTAAGAACA GCAGAACAAT AGACCCCGCC
    AAGATTCCCA AATTCTCCAA GCCAGCGATA GATGAGAAGA TTCAGCTGGG GCCGAGAGCT
    CAGGTGAACT GGATGAAGGG CAGCAGTGGT GGAGCTGGAG CAAAGGCCAG TGAGTCAGAT
    GCGTCTCGGC CGAGTGCCAG TTTGAATCGT TACTCTCCTC TGCAACCCTC GGCACTCCAG
    ACTTCTTCTT TACCCTCCAC ATCTCCAGAT TTTGATTCCA GGAGAGTTCT CGGCAGTCGT
    GGAAGCTCAG GACGGGAGCG CAATGACAAG CCATTGAGTG CGGGTCCTGC TCGGACAGGG
    CCTATTTCAT TGAGCAGTAG CAATAAGGAG ACCCCCGAAG AGCTGGTTCA GGAAGTGTCC
    CGCAGAGACT CCAATGCTTC AGATACACCC AAGCTGCTCG TCAGTACTGC AGACAAGAGC
    AGACTGGAGA ACAGCCAACC CAGAGAGTCT GCTGTTAAAC TTGAAGCACT CTCAGGGCCC
    TCTCCAGATA AACCTGCATT ATCAGAAGAG GAGATGGAGA GGCGGTCCAA GTCTATAATT
    GATGAGTTTT TGCACATTAA TGATTACAAG GAAGCTTTGC AGTGTGTTGA GGAACTAGAG
    CAGTCTGCCA TGCTCTATGT GTTTGTGCGG GTGGGTGTGG AGTCCACGCT GGAAAGGAGT
    CAGATCACGC GAGACCATAT GGGTCAGCTG CTCTTCCAGC TTCTGCAGGC TGGAGTCCTC
    CCTAAACTTC AGTTCTTTAA AGGGTTTTCT GAAACTCTTG AATTAGCAGA TGATATGGCC
    ATTGATATTC CCCATATATG GCTGTACCTT GCGGAGCTGG TAACCCCTGT GCTTCGAGAG
    GGGGGAATCT CTATGAGAGA ATTATTTAGT GAATTTAGCA AACCATTACT CCCTGTGGGA
    AGAGCTGGGA TATTATTTTC TGAAATATTG CACCTTCTAT GCAAACAAAT GAGCCATAAG
    AAAGTGGGAT CATTATGGAG GGATTCTGGG TTGAGCTGGG CAGATTTTAT ACCTGAAACA
    GAGGATGTGC ACAGCTTCAT CACAGAACAT AAACTAGAGT TCACTCTGTC TGATGGCTCA
    AACCCGACCG AGGCTGTCTC TAAGAGAACA CTCTCGCCTG AGGAACTGAA CAAGCAACTT
    GAAAAACTTT TACTTGAGGA CATGGTTGGC GATGAACAAA TCTTCGACTG GGTTGAGGCC
    AATCTAGATG AATCAGAAAT GAGTTCAGCT CCTTTCGTCA GAGCTTTAAT GACTGCTGTG
    TGTAAAGCAG CAGTAAAAAC TGAGGGCTCC TCGTGTAAAG TGGACTTGTC CATAATTCAG
    ACGAGATTGC CTGTATTACA CAAGTACCTT AACTCAGACA CAGAGAGACA GTTGCAAGCA
    CTTTATGCAC TTCAAGCATT GATAGTAAAA CTGGACCAGC CTGCTAATTT GCTCCGGATG
    TTTTTTGACT GTTTGTATGA TGAGGACGTG ATCTCTGAAG ACGCTTTCTA TAAATGGGAG
    GTCAGTAAAG ACCCCGCTGA ACAGCAAGGC AAAGGTGTTG CCCTGAAGTC CGTCACAGCC
    TTCTTCACGT GGCTGCGCGA GGCCGAGGAA GAGTCCGAGG ACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45535
    Clone ID Related Accession (Same CDS sequence) XM_009296899.2 , XM_009296899.3
    Accession Version XM_009296899.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296899.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACTAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CAACAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTGCCCCT GGGCCTCGTC CTACCCATCA CCAGGGAGGA
    TTCAGATCCA TTCAGTTTTT CCAGAGGACG CAGATGCAAA CGGCCCGGCC CACCATTCCA
    AGCAACACCC CACCCATTCG GCCCACGTCT CAGACCCCCA CGGCAGCAGT ATATTCTCCC
    AATCAGCACA TCATGATGAC CATGGCTCAC ATGCCCTTTC ATTCCCCACA GACAGCGCAG
    TACTACATCC CTCAGTATCG TCACAGTGCT CCACAGTATG TGGGACCTCC TCAACAGTAC
    CCTGTCCAGC CGACTGGACC CAGTACCTTC TATGCTGCAG CCAGTCCAGG GGAGTTTCCT
    GCACCTTATG CAGCAGGACC TCCCTACTAT CCTGGGCAGC CCGTGTACAC ACCATCTCCA
    CCCATTATAG TGCCTACACC ACAACAACCT CCACCTGCTA AGAGGGAAAA GAAAACAATC
    CGCATCCGAG ACCCTAACCA GGGTGGCAAG GATGTCACAG ACGAAATATT ATCAGGAGTG
    GGTTTGAGTC GAAACCCAAC CCCGCCTGTG GGACGGCCCT CTTCCACTCC TACACCTCCA
    CAGCTGAACA GCCAGGTAGC AGATCATGGG CACATCATGT ATAATGTGGA CAGCAGCCCG
    CACCTTCCAG CATCCTTCAA CTTGAAAGCA GACGATAAGC CAAAACTAGA GTTTTCCTTG
    CAAAGAACTG CATCTCCTGG TCTGCGGCAG CCCGACACTC CTCTGGAGAG AAGAGATCCC
    AGCAGCCCAG TCCAAACACC CTCCTCTCCC CCACACAAAC CTGAGCTTCC CCCCTCCGAC
    TCAGAGACGG CCTCAAGTGT AGCTACAGCC CCGACTCCTT CCATACCTGC CTCAACAGAA
    GAGTCAGCTG ATGCCCCGAG CCCATTAGCT GAGCCCAGTC TCACTAAAGC TATCACACCT
    GAACCAGAGA GCTCTGAGCC AGAGAAATCC TCCTCACCAC CACCTCAAAG TCTGTCTGGA
    TCCCTTACCC AGCACGAAAA AGCCGTCAAT GGGCTCACGG ATGTTGACGC TGCTCCACTG
    AGTGAAGAAT TGGAAACCCA ACCCAGGGAA GCCTCTCCAT TGCTGCCTAC TTCCAGTGTG
    CCCCAGTCAG AGCCCAGGCC AGTCACACCG GTGCTGGAGG AAGAGTCTGA TCCAATCAAT
    ATGAACAGTC CTTTGCCTCC TGTTGAGGAC GATGCTGGAT GTCCTGACAA CGTTTCCCCC
    TCCCTCTCCA CCTCCACGAC TGCTGCTATC TCCACCACTC CTCCTGCTCC TCCTCCAGGA
    CTCTCGCACC CCAGTCAGGT TTCTGCAGCG TTGGACAGGA GACCCTCTAA TGGAGCAGAG
    ATCAAAGAAA CAGGGAAGGA AAACGAGGCC TTGCCGGACA AGAGAGGAGA GCCTTTCTTA
    CAGTCCAGAA AAAGCTCAAA TCAAGCTACA TCTAGTGCAC CAAAGACCTG GAAGAAGCCA
    AAAGAGGACA TGCCTGTAGG GCAAGATCAG TGTCCAGACA AAGAGGAGGA CACTGATCAG
    GCCAGTATGG AACCTGCTCC TGAAAGCCCC AGTCCTCCTC CTGTGGAGGT GGAGAGGAGG
    CCATCAGTAG GAGCAGTGCT CGAGGAGAAT GGAGAACAGG AAGTGGAGCC TTTGAGAAAC
    GGTGCTGAAC ACACTTCTGA AACTGAGAGC AGTGACAGCA TCCACCCGCC TGCAGTCAAA
    GAGCCGTTGA TCAAAGTATT GCCACAGCCT GCAGAGAAGA ATGGAAAGAA GCAGTACAAC
    AGAGACTTCC TGCTGGGCTT CCAGTTTATG CCTGCATGTG TGCAGAAACC AGAAGGGCTT
    CCTCCCATTT CTGATGTTGT TCTAGATAAG ATTAACCAAA ATAAGCTGCC CATGCGGCCG
    GTAGACCCAC GTGTGCTTTC CAGAGGCCCG GACTTTACGC CAGCGTTTGC GGATTTCAGC
    CGACCCATTT CAGGAGGCAG AGGGGCACCG TTGCTGAATG TTGGCATGCG ACGGCCACCT
    GGACGCAAGA TCATCACAAA CGTGTCTGTG ATTGATGAGG TGCAGCTGAA GACAACAGAG
    AATGCCTGGA AGCCTGGCAT GAAGCGGGAA GGTGCAGTTG AAGATCCAGA TGCCCAGAGA
    ACTCAGGAGC TTTTCCGAAA AGTGCGCAGT ATTCTCAATA AACTGACACC GCAGATGTTC
    AGCCAGCTGA TGAAGCAGGT GACTGACCTC ACCATCGACA CAGAGGAGCG ACTCAAGGGT
    GTCATAGATC TGGTCTTTGA AAAAGCAATC AATGAGCCCA GCTTCTCTGT GGCCTACGGC
    AACATGTGCA GCTGTCTGGC CACGTTAAAA GTGCCCATGA CGGATAAGCC CAACAGCACT
    GTGAATTTCC GGAAGCTGCT TCTGAACCGC TGTCAGAAAG AGTTTGAGAA GGACAAGATG
    GATGACGATG CCTTTGAGAA GAAACACAGG GAGCTGGAGG CAGCTACTGC GTCCAGTGAG
    CGGGAGAGGC TTCAGGAGGA GCTGGAAGAG GCCAAAGACA AGGCCCGAAG ACGCTCCATT
    GGCAACATTA AGTTCATCGG CGAGCTCTTC AAGCTGCGGA TGCTGACAGA GGCCATCATG
    CATGACTGCG TGGTGAAGTT GCTGAAAAAC CATGATGAGG AATCACTGGA GTGCCTGTGT
    AGACTGCTCA CCACTATTGG AAAGGACCTG GACTTTGAGA AAGCTAAGCC ACGAATGGAT
    CAGTACTTTA ATCAGATGGA GAAGATAGTT AAGGAGAGGA AGACTTCTTC ACGCATTCGC
    TTCATGCTGC AGGATGTTAT TGATCTGCGA TTGCACAACT GGGTTTCTCG AAGAGCAGAT
    CAGGGCCCCA AGACCATCGA GCAGATCCAT AAAGACGCAA AGCTGGAGGA GCAGGAGGAA
    CAGAGGAAAG TCCACCAGCA ACTGCTTTCC AAAGACAACA AGAGGAGACC AGTGGTGCAG
    AGAGAGGAGA CCTGGAGCAC TGTGCCTATG ACTAAGAACA GCAGAACAAT AGACCCCGCC
    AAGATTCCCA AATTCTCCAA GCCAGCGATA GATGAGAAGA TTCAGCTGGG GCCGAGAGCT
    CAGGTGAACT GGATGAAGGG CAGCAGTGGT GGAGCTGGAG CAAAGGCCAG TGAGTCAGAT
    GCGTCTCGGC CGAGTGCCAG TTTGAATCGT TACTCTCCTC TGCAACCCTC GGCACTCCAG
    ACTTCTTCTT TACCCTCCAC ATCTCCAGAT TTTGATTCCA GGAGAGTTCT CGGCAGTCGT
    GGAAGCTCAG GACGGGAGCG CAATGACAAG CCATTGAGTG CGGGTCCTGC TCGGACAGGG
    CCTATTTCAT TGAGCAGTAG CAATAAGGAG ACCCCCGAAG AGCTGGTTCA GGAAGTGTCC
    CGCAGAGACT CCAATGCTTC AGATACACCC AAGCTGCTCG TCAGTACTGC AGACAAGAGC
    AGACTGGAGA ACAGCCAACC CAGAGAGTCT GCTGTTAAAC TTGAAGCACT CTCAGGGCCC
    TCTCCAGATA AACCTGCATT ATCAGAAGAG GAGATGGAGA GGCGGTCCAA GTCTATAATT
    GATGAGTTTT TGCACATTAA TGATTACAAG GAAGCTTTGC AGTGTGTTGA GGAACTAGAG
    CAGTCTGCCA TGCTCTATGT GTTTGTGCGG GTGGGTGTGG AGTCCACGCT GGAAAGGAGT
    CAGATCACGC GAGACCATAT GGGTCAGCTG CTCTTCCAGC TTCTGCAGGC TGGAGTCCTC
    CCTAAACTTC AGTTCTTTAA AGGGTTTTCT GAAACTCTTG AATTAGCAGA TGATATGGCC
    ATTGATATTC CCCATATATG GCTGTACCTT GCGGAGCTGG TAACCCCTGT GCTTCGAGAG
    GGGGGAATCT CTATGAGAGA ATTATTTAGT GAATTTAGCA AACCATTACT CCCTGTGGGA
    AGAGCTGGGA TATTATTTTC TGAAATATTG CACCTTCTAT GCAAACAAAT GAGCCATAAG
    AAAGTGGGAT CATTATGGAG GGATTCTGGG TTGAGCTGGG CAGATTTTAT ACCTGAAACA
    GAGGATGTGC ACAGCTTCAT CACAGAACAT AAACTAGAGT TCACTCTGTC TGATGGCTCA
    AACCCGACCG AGGCTGTCTC TAAGAGAACA CTCTCGCCTG AGGAACTGAA CAAGCAACTT
    GAAAAACTTT TACTTGAGGA CATGGTTGGC GATGAACAAA TCTTCGACTG GGTTGAGGCC
    AATCTAGATG AATCAGAAAT GAGTTCAGCT CCTTTCGTCA GAGCTTTAAT GACTGCTGTG
    TGTAAAGCAG CAGTAAAAAC TGAGGGCTCC TCGTGTAAAG TGGACTTGTC CATAATTCAG
    ACGAGATTGC CTGTATTACA CAAGTACCTT AACTCAGACA CAGAGAGACA GTTGCAAGCA
    CTTTATGCAC TTCAAGCATT GATAGTAAAA CTGGACCAGC CTGCTAATTT GCTCCGGATG
    TTTTTTGACT GTTTGTATGA TGAGGACGTG ATCTCTGAAG ACGCTTTCTA TAAATGGGAG
    GTCAGTAAAG ACCCCGCTGA ACAGCAAGGC AAAGGTGTTG CCCTGAAGTC CGTCACAGCC
    TTCTTCACGT GGCTGCGCGA GGCCGAGGAA GAGTCCGAGG ACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295174.1
    CDS458..5605
    Translation

    Target ORF information:

    RefSeq Version XM_009296899.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296899.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACTAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CAACAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTGCCCCT GGGCCTCGTC CTACCCATCA CCAGGGAGGA
    TTCAGATCCA TTCAGTTTTT CCAGAGGACG CAGATGCAAA CGGCCCGGCC CACCATTCCA
    AGCAACACCC CACCCATTCG GCCCACGTCT CAGACCCCCA CGGCAGCAGT ATATTCTCCC
    AATCAGCACA TCATGATGAC CATGGCTCAC ATGCCCTTTC ATTCCCCACA GACAGCGCAG
    TACTACATCC CTCAGTATCG TCACAGTGCT CCACAGTATG TGGGACCTCC TCAACAGTAC
    CCTGTCCAGC CGACTGGACC CAGTACCTTC TATGCTGCAG CCAGTCCAGG GGAGTTTCCT
    GCACCTTATG CAGCAGGACC TCCCTACTAT CCTGGGCAGC CCGTGTACAC ACCATCTCCA
    CCCATTATAG TGCCTACACC ACAACAACCT CCACCTGCTA AGAGGGAAAA GAAAACAATC
    CGCATCCGAG ACCCTAACCA GGGTGGCAAG GATGTCACAG ACGAAATATT ATCAGGAGTG
    GGTTTGAGTC GAAACCCAAC CCCGCCTGTG GGACGGCCCT CTTCCACTCC TACACCTCCA
    CAGCTGAACA GCCAGGTAGC AGATCATGGG CACATCATGT ATAATGTGGA CAGCAGCCCG
    CACCTTCCAG CATCCTTCAA CTTGAAAGCA GACGATAAGC CAAAACTAGA GTTTTCCTTG
    CAAAGAACTG CATCTCCTGG TCTGCGGCAG CCCGACACTC CTCTGGAGAG AAGAGATCCC
    AGCAGCCCAG TCCAAACACC CTCCTCTCCC CCACACAAAC CTGAGCTTCC CCCCTCCGAC
    TCAGAGACGG CCTCAAGTGT AGCTACAGCC CCGACTCCTT CCATACCTGC CTCAACAGAA
    GAGTCAGCTG ATGCCCCGAG CCCATTAGCT GAGCCCAGTC TCACTAAAGC TATCACACCT
    GAACCAGAGA GCTCTGAGCC AGAGAAATCC TCCTCACCAC CACCTCAAAG TCTGTCTGGA
    TCCCTTACCC AGCACGAAAA AGCCGTCAAT GGGCTCACGG ATGTTGACGC TGCTCCACTG
    AGTGAAGAAT TGGAAACCCA ACCCAGGGAA GCCTCTCCAT TGCTGCCTAC TTCCAGTGTG
    CCCCAGTCAG AGCCCAGGCC AGTCACACCG GTGCTGGAGG AAGAGTCTGA TCCAATCAAT
    ATGAACAGTC CTTTGCCTCC TGTTGAGGAC GATGCTGGAT GTCCTGACAA CGTTTCCCCC
    TCCCTCTCCA CCTCCACGAC TGCTGCTATC TCCACCACTC CTCCTGCTCC TCCTCCAGGA
    CTCTCGCACC CCAGTCAGGT TTCTGCAGCG TTGGACAGGA GACCCTCTAA TGGAGCAGAG
    ATCAAAGAAA CAGGGAAGGA AAACGAGGCC TTGCCGGACA AGAGAGGAGA GCCTTTCTTA
    CAGTCCAGAA AAAGCTCAAA TCAAGCTACA TCTAGTGCAC CAAAGACCTG GAAGAAGCCA
    AAAGAGGACA TGCCTGTAGG GCAAGATCAG TGTCCAGACA AAGAGGAGGA CACTGATCAG
    GCCAGTATGG AACCTGCTCC TGAAAGCCCC AGTCCTCCTC CTGTGGAGGT GGAGAGGAGG
    CCATCAGTAG GAGCAGTGCT CGAGGAGAAT GGAGAACAGG AAGTGGAGCC TTTGAGAAAC
    GGTGCTGAAC ACACTTCTGA AACTGAGAGC AGTGACAGCA TCCACCCGCC TGCAGTCAAA
    GAGCCGTTGA TCAAAGTATT GCCACAGCCT GCAGAGAAGA ATGGAAAGAA GCAGTACAAC
    AGAGACTTCC TGCTGGGCTT CCAGTTTATG CCTGCATGTG TGCAGAAACC AGAAGGGCTT
    CCTCCCATTT CTGATGTTGT TCTAGATAAG ATTAACCAAA ATAAGCTGCC CATGCGGCCG
    GTAGACCCAC GTGTGCTTTC CAGAGGCCCG GACTTTACGC CAGCGTTTGC GGATTTCAGC
    CGACCCATTT CAGGAGGCAG AGGGGCACCG TTGCTGAATG TTGGCATGCG ACGGCCACCT
    GGACGCAAGA TCATCACAAA CGTGTCTGTG ATTGATGAGG TGCAGCTGAA GACAACAGAG
    AATGCCTGGA AGCCTGGCAT GAAGCGGGAA GGTGCAGTTG AAGATCCAGA TGCCCAGAGA
    ACTCAGGAGC TTTTCCGAAA AGTGCGCAGT ATTCTCAATA AACTGACACC GCAGATGTTC
    AGCCAGCTGA TGAAGCAGGT GACTGACCTC ACCATCGACA CAGAGGAGCG ACTCAAGGGT
    GTCATAGATC TGGTCTTTGA AAAAGCAATC AATGAGCCCA GCTTCTCTGT GGCCTACGGC
    AACATGTGCA GCTGTCTGGC CACGTTAAAA GTGCCCATGA CGGATAAGCC CAACAGCACT
    GTGAATTTCC GGAAGCTGCT TCTGAACCGC TGTCAGAAAG AGTTTGAGAA GGACAAGATG
    GATGACGATG CCTTTGAGAA GAAACACAGG GAGCTGGAGG CAGCTACTGC GTCCAGTGAG
    CGGGAGAGGC TTCAGGAGGA GCTGGAAGAG GCCAAAGACA AGGCCCGAAG ACGCTCCATT
    GGCAACATTA AGTTCATCGG CGAGCTCTTC AAGCTGCGGA TGCTGACAGA GGCCATCATG
    CATGACTGCG TGGTGAAGTT GCTGAAAAAC CATGATGAGG AATCACTGGA GTGCCTGTGT
    AGACTGCTCA CCACTATTGG AAAGGACCTG GACTTTGAGA AAGCTAAGCC ACGAATGGAT
    CAGTACTTTA ATCAGATGGA GAAGATAGTT AAGGAGAGGA AGACTTCTTC ACGCATTCGC
    TTCATGCTGC AGGATGTTAT TGATCTGCGA TTGCACAACT GGGTTTCTCG AAGAGCAGAT
    CAGGGCCCCA AGACCATCGA GCAGATCCAT AAAGACGCAA AGCTGGAGGA GCAGGAGGAA
    CAGAGGAAAG TCCACCAGCA ACTGCTTTCC AAAGACAACA AGAGGAGACC AGTGGTGCAG
    AGAGAGGAGA CCTGGAGCAC TGTGCCTATG ACTAAGAACA GCAGAACAAT AGACCCCGCC
    AAGATTCCCA AATTCTCCAA GCCAGCGATA GATGAGAAGA TTCAGCTGGG GCCGAGAGCT
    CAGGTGAACT GGATGAAGGG CAGCAGTGGT GGAGCTGGAG CAAAGGCCAG TGAGTCAGAT
    GCGTCTCGGC CGAGTGCCAG TTTGAATCGT TACTCTCCTC TGCAACCCTC GGCACTCCAG
    ACTTCTTCTT TACCCTCCAC ATCTCCAGAT TTTGATTCCA GGAGAGTTCT CGGCAGTCGT
    GGAAGCTCAG GACGGGAGCG CAATGACAAG CCATTGAGTG CGGGTCCTGC TCGGACAGGG
    CCTATTTCAT TGAGCAGTAG CAATAAGGAG ACCCCCGAAG AGCTGGTTCA GGAAGTGTCC
    CGCAGAGACT CCAATGCTTC AGATACACCC AAGCTGCTCG TCAGTACTGC AGACAAGAGC
    AGACTGGAGA ACAGCCAACC CAGAGAGTCT GCTGTTAAAC TTGAAGCACT CTCAGGGCCC
    TCTCCAGATA AACCTGCATT ATCAGAAGAG GAGATGGAGA GGCGGTCCAA GTCTATAATT
    GATGAGTTTT TGCACATTAA TGATTACAAG GAAGCTTTGC AGTGTGTTGA GGAACTAGAG
    CAGTCTGCCA TGCTCTATGT GTTTGTGCGG GTGGGTGTGG AGTCCACGCT GGAAAGGAGT
    CAGATCACGC GAGACCATAT GGGTCAGCTG CTCTTCCAGC TTCTGCAGGC TGGAGTCCTC
    CCTAAACTTC AGTTCTTTAA AGGGTTTTCT GAAACTCTTG AATTAGCAGA TGATATGGCC
    ATTGATATTC CCCATATATG GCTGTACCTT GCGGAGCTGG TAACCCCTGT GCTTCGAGAG
    GGGGGAATCT CTATGAGAGA ATTATTTAGT GAATTTAGCA AACCATTACT CCCTGTGGGA
    AGAGCTGGGA TATTATTTTC TGAAATATTG CACCTTCTAT GCAAACAAAT GAGCCATAAG
    AAAGTGGGAT CATTATGGAG GGATTCTGGG TTGAGCTGGG CAGATTTTAT ACCTGAAACA
    GAGGATGTGC ACAGCTTCAT CACAGAACAT AAACTAGAGT TCACTCTGTC TGATGGCTCA
    AACCCGACCG AGGCTGTCTC TAAGAGAACA CTCTCGCCTG AGGAACTGAA CAAGCAACTT
    GAAAAACTTT TACTTGAGGA CATGGTTGGC GATGAACAAA TCTTCGACTG GGTTGAGGCC
    AATCTAGATG AATCAGAAAT GAGTTCAGCT CCTTTCGTCA GAGCTTTAAT GACTGCTGTG
    TGTAAAGCAG CAGTAAAAAC TGAGGGCTCC TCGTGTAAAG TGGACTTGTC CATAATTCAG
    ACGAGATTGC CTGTATTACA CAAGTACCTT AACTCAGACA CAGAGAGACA GTTGCAAGCA
    CTTTATGCAC TTCAAGCATT GATAGTAAAA CTGGACCAGC CTGCTAATTT GCTCCGGATG
    TTTTTTGACT GTTTGTATGA TGAGGACGTG ATCTCTGAAG ACGCTTTCTA TAAATGGGAG
    GTCAGTAAAG ACCCCGCTGA ACAGCAAGGC AAAGGTGTTG CCCTGAAGTC CGTCACAGCC
    TTCTTCACGT GGCTGCGCGA GGCCGAGGAA GAGTCCGAGG ACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45536
    Clone ID Related Accession (Same CDS sequence) XM_009296900.2
    Accession Version XM_009296900.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5127bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296900.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACTAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAATA TCCACGAGTG CCGCCTCCCC TGGACGAGCG AATCTTCTCA
    ACACAGCCTG TCTCTGCTGT CTACAGTGTA CAGAGACCCC CTGGCCCCCC ATTTACTGCA
    CATGAAATCA ACAAGGGACA CCCTAACCTA GCGGCTACCC CGCCGGGTCA TGCCTCTTCC
    CCTGGACTAT CTCAGGTATC AGTTTCAACA GTATCCACTG CGCATCTCTA TGGTCATCCT
    AAAGGCTGGG AGCCAGGAGG AGGGTCTCCG TACACAACGG GACAGAATGC CGGCACGACC
    CCTCTGGTAT ACTCTCCAAC AACCCAGCCA ATGAACGCAC AGCCTCAGAG CCGCCCGTTT
    GCCCCTGGGC CTCGTCCTAC CCATCACCAG GGAGGATTCA GATCCATTCA GTTTTTCCAG
    AGGACGCAGA TGCAAACGGC CCGGCCCACC ATTCCAAGCA ACACCCCACC CATTCGGCCC
    ACGTCTCAGA CCCCCACGGC AGCAGTATAT TCTCCCAATC AGCACATCAT GATGACCATG
    GCTCACATGC CCTTTCATTC CCCACAGACA GCGCAGTACT ACATCCCTCA GTATCGTCAC
    AGTGCTCCAC AGTATGTGGG ACCTCCTCAA CAGTACCCTG TCCAGCCGAC TGGACCCAGT
    ACCTTCTATG CTGCAGCCAG TCCAGGGGAG TTTCCTGCAC CTTATGCAGC AGGACCTCCC
    TACTATCCTG GGCAGCCCGT GTACACACCA TCTCCACCCA TTATAGTGCC TACACCACAA
    CAACCTCCAC CTGCTAAGAG GGAAAAGAAA ACAATCCGCA TCCGAGACCC TAACCAGGGT
    GGCAAGGATG TCACAGACGA AATATTATCA GGAGTGGGTT TGAGTCGAAA CCCAACCCCG
    CCTGTGGGAC GGCCCTCTTC CACTCCTACA CCTCCACAGC AGCTGAACAG CCAGGTAGCA
    GATCATGGGC ACATCATGTA TAATGTGGAC AGCAGCCCGC ACCTTCCAGC ATCCTTCAAC
    TTGAAAGCAG ACGATAAGCC AAAACTAGAG TTTTCCTTGC AAAGAACTGC ATCTCCTGGT
    CTGCGGCAGC CCGACACTCC TCTGGAGAGA AGAGATCCCA GCAGCCCAGT CCAAACACCC
    TCCTCTCCCC CACACAAACC TGAGCTTCCC CCCTCCGACT CAGAGACGGC CTCAAGTGTA
    GCTACAGCCC CGACTCCTTC CATACCTGCC TCAACAGAAG AGTCAGCTGA TGCCCCGAGC
    CCATTAGCTG AGCCCAGTCT CACTAAAGCT ATCACACCTG AACCAGAGAG CTCTGAGCCA
    GAGAAATCCT CCTCACCACC ACCTCAAAGT CTGTCTGGAT CCCTTACCCA GCACGAAAAA
    GCCGTCAATG GGCTCACGGA TGTTGACGCT GCTCCACTGA GTGAAGAATT GGAAACCCAA
    CCCAGGGAAG CCTCTCCATT GCTGCCTACT TCCAGTGTGC CCCAGTCAGA GCCCAGGCCA
    GTCACACCGG TGCTGGAGGA AGAGTCTGAT CCAATCAATA TGAACAGTCC TTTGCCTCCT
    GTTGAGGACG ATGCTGGATG TCCTGACAAC GTTTCCCCCT CCCTCTCCAC CTCCACGACT
    GCTGCTATCT CCACCACTCC TCCTGCTCCT CCTCCAGGAC TCTCGCACCC CAGTCAGGTT
    TCTGCAGCGT TGGACAGGAG ACCCTCTAAT GGAGCAGAGA TCAAAGAAAC AGGGAAGGAA
    AACGAGGCCT TGCCGGACAA GAGAGGAGAG CCTTTCTTAC AGTCCAGAAA AAGCTCAAAT
    CAAGCTACAT CTAGTGCACC AAAGACCTGG AAGAAGCCAA AAGAGGACAT GCCTGTAGGG
    CAAGATCAGT GTCCAGACAA AGAGGAGGAC ACTGATCAGG CCAGTATGGA ACCTGCTCCT
    GAAAGCCCCA GTCCTCCTCC TGTGGAGGTG GAGAGGAGGC CATCAGTAGG AGCAGTGCTC
    GAGGAGAATG GAGAACAGGA AGTGGAGCCT TTGAGAAACG GTGCTGAACA CACTTCTGAA
    ACTGAGAGCA GTGACAGCAT CCACCCGCCT GCAGTCAAAG AGCCGTTGAT CAAAGTATTG
    CCACAGCCTG CAGAGAAGAA TGGAAAGAAG CAGTACAACA GAGACTTCCT GCTGGGCTTC
    CAGTTTATGC CTGCATGTGT GCAGAAACCA GAAGGGCTTC CTCCCATTTC TGATGTTGTT
    CTAGATAAGA TTAACCAAAA TAAGCTGCCC ATGCGGCCGG TAGACCCACG TGTGCTTTCC
    AGAGGCCCGG ACTTTACGCC AGCGTTTGCG GATTTCAGCC GACCCATTTC AGGAGGCAGA
    GGGGCACCGT TGCTGAATGT TGGCATGCGA CGGCCACCTG GACGCAAGAT CATCACAAAC
    GTGTCTGTGA TTGATGAGGT GCAGCTGAAG ACAACAGAGA ATGCCTGGAA GCCTGGCATG
    AAGCGGGAAG GTGCAGTTGA AGATCCAGAT GCCCAGAGAA CTCAGGAGCT TTTCCGAAAA
    GTGCGCAGTA TTCTCAATAA ACTGACACCG CAGATGTTCA GCCAGCTGAT GAAGCAGGTG
    ACTGACCTCA CCATCGACAC AGAGGAGCGA CTCAAGGGTG TCATAGATCT GGTCTTTGAA
    AAAGCAATCA ATGAGCCCAG CTTCTCTGTG GCCTACGGCA ACATGTGCAG CTGTCTGGCC
    ACGTTAAAAG TGCCCATGAC GGATAAGCCC AACAGCACTG TGAATTTCCG GAAGCTGCTT
    CTGAACCGCT GTCAGAAAGA GTTTGAGAAG GACAAGATGG ATGACGATGC CTTTGAGAAG
    AAACACAGGG AGCTGGAGGC AGCTACTGCG TCCAGTGAGC GGGAGAGGCT TCAGGAGGAG
    CTGGAAGAGG CCAAAGACAA GGCCCGAAGA CGCTCCATTG GCAACATTAA GTTCATCGGC
    GAGCTCTTCA AGCTGCGGAT GCTGACAGAG GCCATCATGC ATGACTGCGT GGTGAAGTTG
    CTGAAAAACC ATGATGAGGA ATCACTGGAG TGCCTGTGTA GACTGCTCAC CACTATTGGA
    AAGGACCTGG ACTTTGAGAA AGCTAAGCCA CGAATGGATC AGTACTTTAA TCAGATGGAG
    AAGATAGTTA AGGAGAGGAA GACTTCTTCA CGCATTCGCT TCATGCTGCA GGATGTTATT
    GATCTGCGAT TGCACAACTG GGTTTCTCGA AGAGCAGATC AGGGCCCCAA GACCATCGAG
    CAGATCCATA AAGACGCAAA GCTGGAGGAG CAGGAGGAAC AGAGGAAAGT CCACCAGCAA
    CTGCTTTCCA AAGACAACAA GAGGAGACCA GTGGTGCAGA GAGAGGAGAC CTGGAGCACT
    GTGCCTATGA CTAAGAACAG CAGAACAATA GACCCCGCCA AGATTCCCAA ATTCTCCAAG
    CCAGCGATAG ATGAGAAGAT TCAGCTGGGG CCGAGAGCTC AGGTGAACTG GATGAAGGGC
    AGCAGTGGTG GAGCTGGAGC AAAGGCCAGT GAGTCAGATG CGTCTCGGCC GAGTGCCAGT
    TTGAATCGTT ACTCTCCTCT GCAACCCTCG GCACTCCAGA CTTCTTCTTT ACCCTCCACA
    TCTCCAGATT TTGATTCCAG GAGAGTTCTC GGCAGTCGTG GAAGCTCAGG ACGGGAGCGC
    AATGACAAGC CATTGAGTGC GGGTCCTGCT CGGACAGGGC CTATTTCATT GAGCAGTAGC
    AATAAGGAGA CCCCCGAAGA GCTGGTTCAG GAAGTGTCCC GCAGAGACTC CAATGCTTCA
    GATACACCCA AGCTGCTCGT CAGTACTGCA GACAAGAGCA GACTGGAGAA CAGCCAACCC
    AGAGAGTCTG CTGTTAAACT TGAAGCACTC TCAGGGCCCT CTCCAGATAA ACCTGCATTA
    TCAGAAGAGG AGATGGAGAG GCGGTCCAAG TCTATAATTG ATGAGTTTTT GCACATTAAT
    GATTACAAGG AAGCTTTGCA GTGTGTTGAG GAACTAGAGC AGTCTGCCAT GCTCTATGTG
    TTTGTGCGGG TGGGTGTGGA GTCCACGCTG GAAAGGAGTC AGATCACGCG AGACCATATG
    GGTCAGCTGC TCTTCCAGCT TCTGCAGGCT GGAGTCCTCC CTAAACTTCA GTTCTTTAAA
    GGGTTTTCTG AAACTCTTGA ATTAGCAGAT GATATGGCCA TTGATATTCC CCATATATGG
    CTGTACCTTG CGGAGCTGGT AACCCCTGTG CTTCGAGAGG GGGGAATCTC TATGAGAGAA
    TTATTTAGTG AATTTAGCAA ACCATTACTC CCTGTGGGAA GAGCTGGGAT ATTATTTTCT
    GAAATATTGC ACCTTCTATG CAAACAAATG AGCCATAAGA AAGTGGGATC ATTATGGAGG
    GATTCTGGGT TGAGCTGGGC AGATTTTATA CCTGAAACAG AGGATGTGCA CAGCTTCATC
    ACAGAACATA AACTAGAGTT CACTCTGTCT GATGGCTCAA ACCCGACCGA GGCTGTCTCT
    AAGAGAACAC TCTCGCCTGA GGAACTGAAC AAGCAACTTG AAAAACTTTT ACTTGAGGAC
    ATGGTTGGCG ATGAACAAAT CTTCGACTGG GTTGAGGCCA ATCTAGATGA ATCAGAAATG
    AGTTCAGCTC CTTTCGTCAG AGCTTTAATG ACTGCTGTGT GTAAAGCAGC AGTAAAAACT
    GAGGGCTCCT CGTGTAAAGT GGACTTGTCC ATAATTCAGA CGAGATTGCC TGTATTACAC
    AAGTACCTTA ACTCAGACAC AGAGAGACAG TTGCAAGCAC TTTATGCACT TCAAGCATTG
    ATAGTAAAAC TGGACCAGCC TGCTAATTTG CTCCGGATGT TTTTTGACTG TTTGTATGAT
    GAGGACGTGA TCTCTGAAGA CGCTTTCTAT AAATGGGAGG TCAGTAAAGA CCCCGCTGAA
    CAGCAAGGCA AAGGTGTTGC CCTGAAGTCC GTCACAGCCT TCTTCACGTG GCTGCGCGAG
    GCCGAGGAAG AGTCCGAGGA CAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295175.1
    CDS605..5731
    Translation

    Target ORF information:

    RefSeq Version XM_009296900.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296900.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACTAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAATA TCCACGAGTG CCGCCTCCCC TGGACGAGCG AATCTTCTCA
    ACACAGCCTG TCTCTGCTGT CTACAGTGTA CAGAGACCCC CTGGCCCCCC ATTTACTGCA
    CATGAAATCA ACAAGGGACA CCCTAACCTA GCGGCTACCC CGCCGGGTCA TGCCTCTTCC
    CCTGGACTAT CTCAGGTATC AGTTTCAACA GTATCCACTG CGCATCTCTA TGGTCATCCT
    AAAGGCTGGG AGCCAGGAGG AGGGTCTCCG TACACAACGG GACAGAATGC CGGCACGACC
    CCTCTGGTAT ACTCTCCAAC AACCCAGCCA ATGAACGCAC AGCCTCAGAG CCGCCCGTTT
    GCCCCTGGGC CTCGTCCTAC CCATCACCAG GGAGGATTCA GATCCATTCA GTTTTTCCAG
    AGGACGCAGA TGCAAACGGC CCGGCCCACC ATTCCAAGCA ACACCCCACC CATTCGGCCC
    ACGTCTCAGA CCCCCACGGC AGCAGTATAT TCTCCCAATC AGCACATCAT GATGACCATG
    GCTCACATGC CCTTTCATTC CCCACAGACA GCGCAGTACT ACATCCCTCA GTATCGTCAC
    AGTGCTCCAC AGTATGTGGG ACCTCCTCAA CAGTACCCTG TCCAGCCGAC TGGACCCAGT
    ACCTTCTATG CTGCAGCCAG TCCAGGGGAG TTTCCTGCAC CTTATGCAGC AGGACCTCCC
    TACTATCCTG GGCAGCCCGT GTACACACCA TCTCCACCCA TTATAGTGCC TACACCACAA
    CAACCTCCAC CTGCTAAGAG GGAAAAGAAA ACAATCCGCA TCCGAGACCC TAACCAGGGT
    GGCAAGGATG TCACAGACGA AATATTATCA GGAGTGGGTT TGAGTCGAAA CCCAACCCCG
    CCTGTGGGAC GGCCCTCTTC CACTCCTACA CCTCCACAGC AGCTGAACAG CCAGGTAGCA
    GATCATGGGC ACATCATGTA TAATGTGGAC AGCAGCCCGC ACCTTCCAGC ATCCTTCAAC
    TTGAAAGCAG ACGATAAGCC AAAACTAGAG TTTTCCTTGC AAAGAACTGC ATCTCCTGGT
    CTGCGGCAGC CCGACACTCC TCTGGAGAGA AGAGATCCCA GCAGCCCAGT CCAAACACCC
    TCCTCTCCCC CACACAAACC TGAGCTTCCC CCCTCCGACT CAGAGACGGC CTCAAGTGTA
    GCTACAGCCC CGACTCCTTC CATACCTGCC TCAACAGAAG AGTCAGCTGA TGCCCCGAGC
    CCATTAGCTG AGCCCAGTCT CACTAAAGCT ATCACACCTG AACCAGAGAG CTCTGAGCCA
    GAGAAATCCT CCTCACCACC ACCTCAAAGT CTGTCTGGAT CCCTTACCCA GCACGAAAAA
    GCCGTCAATG GGCTCACGGA TGTTGACGCT GCTCCACTGA GTGAAGAATT GGAAACCCAA
    CCCAGGGAAG CCTCTCCATT GCTGCCTACT TCCAGTGTGC CCCAGTCAGA GCCCAGGCCA
    GTCACACCGG TGCTGGAGGA AGAGTCTGAT CCAATCAATA TGAACAGTCC TTTGCCTCCT
    GTTGAGGACG ATGCTGGATG TCCTGACAAC GTTTCCCCCT CCCTCTCCAC CTCCACGACT
    GCTGCTATCT CCACCACTCC TCCTGCTCCT CCTCCAGGAC TCTCGCACCC CAGTCAGGTT
    TCTGCAGCGT TGGACAGGAG ACCCTCTAAT GGAGCAGAGA TCAAAGAAAC AGGGAAGGAA
    AACGAGGCCT TGCCGGACAA GAGAGGAGAG CCTTTCTTAC AGTCCAGAAA AAGCTCAAAT
    CAAGCTACAT CTAGTGCACC AAAGACCTGG AAGAAGCCAA AAGAGGACAT GCCTGTAGGG
    CAAGATCAGT GTCCAGACAA AGAGGAGGAC ACTGATCAGG CCAGTATGGA ACCTGCTCCT
    GAAAGCCCCA GTCCTCCTCC TGTGGAGGTG GAGAGGAGGC CATCAGTAGG AGCAGTGCTC
    GAGGAGAATG GAGAACAGGA AGTGGAGCCT TTGAGAAACG GTGCTGAACA CACTTCTGAA
    ACTGAGAGCA GTGACAGCAT CCACCCGCCT GCAGTCAAAG AGCCGTTGAT CAAAGTATTG
    CCACAGCCTG CAGAGAAGAA TGGAAAGAAG CAGTACAACA GAGACTTCCT GCTGGGCTTC
    CAGTTTATGC CTGCATGTGT GCAGAAACCA GAAGGGCTTC CTCCCATTTC TGATGTTGTT
    CTAGATAAGA TTAACCAAAA TAAGCTGCCC ATGCGGCCGG TAGACCCACG TGTGCTTTCC
    AGAGGCCCGG ACTTTACGCC AGCGTTTGCG GATTTCAGCC GACCCATTTC AGGAGGCAGA
    GGGGCACCGT TGCTGAATGT TGGCATGCGA CGGCCACCTG GACGCAAGAT CATCACAAAC
    GTGTCTGTGA TTGATGAGGT GCAGCTGAAG ACAACAGAGA ATGCCTGGAA GCCTGGCATG
    AAGCGGGAAG GTGCAGTTGA AGATCCAGAT GCCCAGAGAA CTCAGGAGCT TTTCCGAAAA
    GTGCGCAGTA TTCTCAATAA ACTGACACCG CAGATGTTCA GCCAGCTGAT GAAGCAGGTG
    ACTGACCTCA CCATCGACAC AGAGGAGCGA CTCAAGGGTG TCATAGATCT GGTCTTTGAA
    AAAGCAATCA ATGAGCCCAG CTTCTCTGTG GCCTACGGCA ACATGTGCAG CTGTCTGGCC
    ACGTTAAAAG TGCCCATGAC GGATAAGCCC AACAGCACTG TGAATTTCCG GAAGCTGCTT
    CTGAACCGCT GTCAGAAAGA GTTTGAGAAG GACAAGATGG ATGACGATGC CTTTGAGAAG
    AAACACAGGG AGCTGGAGGC AGCTACTGCG TCCAGTGAGC GGGAGAGGCT TCAGGAGGAG
    CTGGAAGAGG CCAAAGACAA GGCCCGAAGA CGCTCCATTG GCAACATTAA GTTCATCGGC
    GAGCTCTTCA AGCTGCGGAT GCTGACAGAG GCCATCATGC ATGACTGCGT GGTGAAGTTG
    CTGAAAAACC ATGATGAGGA ATCACTGGAG TGCCTGTGTA GACTGCTCAC CACTATTGGA
    AAGGACCTGG ACTTTGAGAA AGCTAAGCCA CGAATGGATC AGTACTTTAA TCAGATGGAG
    AAGATAGTTA AGGAGAGGAA GACTTCTTCA CGCATTCGCT TCATGCTGCA GGATGTTATT
    GATCTGCGAT TGCACAACTG GGTTTCTCGA AGAGCAGATC AGGGCCCCAA GACCATCGAG
    CAGATCCATA AAGACGCAAA GCTGGAGGAG CAGGAGGAAC AGAGGAAAGT CCACCAGCAA
    CTGCTTTCCA AAGACAACAA GAGGAGACCA GTGGTGCAGA GAGAGGAGAC CTGGAGCACT
    GTGCCTATGA CTAAGAACAG CAGAACAATA GACCCCGCCA AGATTCCCAA ATTCTCCAAG
    CCAGCGATAG ATGAGAAGAT TCAGCTGGGG CCGAGAGCTC AGGTGAACTG GATGAAGGGC
    AGCAGTGGTG GAGCTGGAGC AAAGGCCAGT GAGTCAGATG CGTCTCGGCC GAGTGCCAGT
    TTGAATCGTT ACTCTCCTCT GCAACCCTCG GCACTCCAGA CTTCTTCTTT ACCCTCCACA
    TCTCCAGATT TTGATTCCAG GAGAGTTCTC GGCAGTCGTG GAAGCTCAGG ACGGGAGCGC
    AATGACAAGC CATTGAGTGC GGGTCCTGCT CGGACAGGGC CTATTTCATT GAGCAGTAGC
    AATAAGGAGA CCCCCGAAGA GCTGGTTCAG GAAGTGTCCC GCAGAGACTC CAATGCTTCA
    GATACACCCA AGCTGCTCGT CAGTACTGCA GACAAGAGCA GACTGGAGAA CAGCCAACCC
    AGAGAGTCTG CTGTTAAACT TGAAGCACTC TCAGGGCCCT CTCCAGATAA ACCTGCATTA
    TCAGAAGAGG AGATGGAGAG GCGGTCCAAG TCTATAATTG ATGAGTTTTT GCACATTAAT
    GATTACAAGG AAGCTTTGCA GTGTGTTGAG GAACTAGAGC AGTCTGCCAT GCTCTATGTG
    TTTGTGCGGG TGGGTGTGGA GTCCACGCTG GAAAGGAGTC AGATCACGCG AGACCATATG
    GGTCAGCTGC TCTTCCAGCT TCTGCAGGCT GGAGTCCTCC CTAAACTTCA GTTCTTTAAA
    GGGTTTTCTG AAACTCTTGA ATTAGCAGAT GATATGGCCA TTGATATTCC CCATATATGG
    CTGTACCTTG CGGAGCTGGT AACCCCTGTG CTTCGAGAGG GGGGAATCTC TATGAGAGAA
    TTATTTAGTG AATTTAGCAA ACCATTACTC CCTGTGGGAA GAGCTGGGAT ATTATTTTCT
    GAAATATTGC ACCTTCTATG CAAACAAATG AGCCATAAGA AAGTGGGATC ATTATGGAGG
    GATTCTGGGT TGAGCTGGGC AGATTTTATA CCTGAAACAG AGGATGTGCA CAGCTTCATC
    ACAGAACATA AACTAGAGTT CACTCTGTCT GATGGCTCAA ACCCGACCGA GGCTGTCTCT
    AAGAGAACAC TCTCGCCTGA GGAACTGAAC AAGCAACTTG AAAAACTTTT ACTTGAGGAC
    ATGGTTGGCG ATGAACAAAT CTTCGACTGG GTTGAGGCCA ATCTAGATGA ATCAGAAATG
    AGTTCAGCTC CTTTCGTCAG AGCTTTAATG ACTGCTGTGT GTAAAGCAGC AGTAAAAACT
    GAGGGCTCCT CGTGTAAAGT GGACTTGTCC ATAATTCAGA CGAGATTGCC TGTATTACAC
    AAGTACCTTA ACTCAGACAC AGAGAGACAG TTGCAAGCAC TTTATGCACT TCAAGCATTG
    ATAGTAAAAC TGGACCAGCC TGCTAATTTG CTCCGGATGT TTTTTGACTG TTTGTATGAT
    GAGGACGTGA TCTCTGAAGA CGCTTTCTAT AAATGGGAGG TCAGTAAAGA CCCCGCTGAA
    CAGCAAGGCA AAGGTGTTGC CCTGAAGTCC GTCACAGCCT TCTTCACGTG GCTGCGCGAG
    GCCGAGGAAG AGTCCGAGGA CAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45537
    Clone ID Related Accession (Same CDS sequence) XM_009296901.2
    Accession Version XM_009296901.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5097bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296901.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACTAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CAACAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTTTCCAG AGGACGCAGA TGCAAACGGC CCGGCCCACC
    ATTCCAAGCA ACACCCCACC CATTCGGCCC ACGTCTCAGA CCCCCACGGC AGCAGTATAT
    TCTCCCAATC AGCACATCAT GATGACCATG GCTCACATGC CCTTTCATTC CCCACAGACA
    GCGCAGTACT ACATCCCTCA GTATCGTCAC AGTGCTCCAC AGTATGTGGG ACCTCCTCAA
    CAGTACCCTG TCCAGCCGAC TGGACCCAGT ACCTTCTATG CTGCAGCCAG TCCAGGGGAG
    TTTCCTGCAC CTTATGCAGC AGGACCTCCC TACTATCCTG GGCAGCCCGT GTACACACCA
    TCTCCACCCA TTATAGTGCC TACACCACAA CAACCTCCAC CTGCTAAGAG GGAAAAGAAA
    ACAATCCGCA TCCGAGACCC TAACCAGGGT GGCAAGGATG TCACAGACGA AATATTATCA
    GGAGTGGGTT TGAGTCGAAA CCCAACCCCG CCTGTGGGAC GGCCCTCTTC CACTCCTACA
    CCTCCACAGC AGCTGAACAG CCAGGTAGCA GATCATGGGC ACATCATGTA TAATGTGGAC
    AGCAGCCCGC ACCTTCCAGC ATCCTTCAAC TTGAAAGCAG ACGATAAGCC AAAACTAGAG
    TTTTCCTTGC AAAGAACTGC ATCTCCTGGT CTGCGGCAGC CCGACACTCC TCTGGAGAGA
    AGAGATCCCA GCAGCCCAGT CCAAACACCC TCCTCTCCCC CACACAAACC TGAGCTTCCC
    CCCTCCGACT CAGAGACGGC CTCAAGTGTA GCTACAGCCC CGACTCCTTC CATACCTGCC
    TCAACAGAAG AGTCAGCTGA TGCCCCGAGC CCATTAGCTG AGCCCAGTCT CACTAAAGCT
    ATCACACCTG AACCAGAGAG CTCTGAGCCA GAGAAATCCT CCTCACCACC ACCTCAAAGT
    CTGTCTGGAT CCCTTACCCA GCACGAAAAA GCCGTCAATG GGCTCACGGA TGTTGACGCT
    GCTCCACTGA GTGAAGAATT GGAAACCCAA CCCAGGGAAG CCTCTCCATT GCTGCCTACT
    TCCAGTGTGC CCCAGTCAGA GCCCAGGCCA GTCACACCGG TGCTGGAGGA AGAGTCTGAT
    CCAATCAATA TGAACAGTCC TTTGCCTCCT GTTGAGGACG ATGCTGGATG TCCTGACAAC
    GTTTCCCCCT CCCTCTCCAC CTCCACGACT GCTGCTATCT CCACCACTCC TCCTGCTCCT
    CCTCCAGGAC TCTCGCACCC CAGTCAGGTT TCTGCAGCGT TGGACAGGAG ACCCTCTAAT
    GGAGCAGAGA TCAAAGAAAC AGGGAAGGAA AACGAGGCCT TGCCGGACAA GAGAGGAGAG
    CCTTTCTTAC AGTCCAGAAA AAGCTCAAAT CAAGCTACAT CTAGTGCACC AAAGACCTGG
    AAGAAGCCAA AAGAGGACAT GCCTGTAGGG CAAGATCAGT GTCCAGACAA AGAGGAGGAC
    ACTGATCAGG CCAGTATGGA ACCTGCTCCT GAAAGCCCCA GTCCTCCTCC TGTGGAGGTG
    GAGAGGAGGC CATCAGTAGG AGCAGTGCTC GAGGAGAATG GAGAACAGGA AGTGGAGCCT
    TTGAGAAACG GTGCTGAACA CACTTCTGAA ACTGAGAGCA GTGACAGCAT CCACCCGCCT
    GCAGTCAAAG AGCCGTTGAT CAAAGTATTG CCACAGCCTG CAGAGAAGAA TGGAAAGAAG
    CAGTACAACA GAGACTTCCT GCTGGGCTTC CAGTTTATGC CTGCATGTGT GCAGAAACCA
    GAAGGGCTTC CTCCCATTTC TGATGTTGTT CTAGATAAGA TTAACCAAAA TAAGCTGCCC
    ATGCGGCCGG TAGACCCACG TGTGCTTTCC AGAGGCCCGG ACTTTACGCC AGCGTTTGCG
    GATTTCAGCC GACCCATTTC AGGAGGCAGA GGGGCACCGT TGCTGAATGT TGGCATGCGA
    CGGCCACCTG GACGCAAGAT CATCACAAAC GTGTCTGTGA TTGATGAGGT GCAGCTGAAG
    ACAACAGAGA ATGCCTGGAA GCCTGGCATG AAGCGGGAAG GTGCAGTTGA AGATCCAGAT
    GCCCAGAGAA CTCAGGAGCT TTTCCGAAAA GTGCGCAGTA TTCTCAATAA ACTGACACCG
    CAGATGTTCA GCCAGCTGAT GAAGCAGGTG ACTGACCTCA CCATCGACAC AGAGGAGCGA
    CTCAAGGGTG TCATAGATCT GGTCTTTGAA AAAGCAATCA ATGAGCCCAG CTTCTCTGTG
    GCCTACGGCA ACATGTGCAG CTGTCTGGCC ACGTTAAAAG TGCCCATGAC GGATAAGCCC
    AACAGCACTG TGAATTTCCG GAAGCTGCTT CTGAACCGCT GTCAGAAAGA GTTTGAGAAG
    GACAAGATGG ATGACGATGC CTTTGAGAAG AAACACAGGG AGCTGGAGGC AGCTACTGCG
    TCCAGTGAGC GGGAGAGGCT TCAGGAGGAG CTGGAAGAGG CCAAAGACAA GGCCCGAAGA
    CGCTCCATTG GCAACATTAA GTTCATCGGC GAGCTCTTCA AGCTGCGGAT GCTGACAGAG
    GCCATCATGC ATGACTGCGT GGTGAAGTTG CTGAAAAACC ATGATGAGGA ATCACTGGAG
    TGCCTGTGTA GACTGCTCAC CACTATTGGA AAGGACCTGG ACTTTGAGAA AGCTAAGCCA
    CGAATGGATC AGTACTTTAA TCAGATGGAG AAGATAGTTA AGGAGAGGAA GACTTCTTCA
    CGCATTCGCT TCATGCTGCA GGATGTTATT GATCTGCGAT TGCACAACTG GGTTTCTCGA
    AGAGCAGATC AGGGCCCCAA GACCATCGAG CAGATCCATA AAGACGCAAA GCTGGAGGAG
    CAGGAGGAAC AGAGGAAAGT CCACCAGCAA CTGCTTTCCA AAGACAACAA GAGGAGACCA
    GTGGTGCAGA GAGAGGAGAC CTGGAGCACT GTGCCTATGA CTAAGAACAG CAGAACAATA
    GACCCCGCCA AGATTCCCAA ATTCTCCAAG CCAGCGATAG ATGAGAAGAT TCAGCTGGGG
    CCGAGAGCTC AGGTGAACTG GATGAAGGGC AGCAGTGGTG GAGCTGGAGC AAAGGCCAGT
    GAGTCAGATG CGTCTCGGCC GAGTGCCAGT TTGAATCGTT ACTCTCCTCT GCAACCCTCG
    GCACTCCAGA CTTCTTCTTT ACCCTCCACA TCTCCAGATT TTGATTCCAG GAGAGTTCTC
    GGCAGTCGTG GAAGCTCAGG ACGGGAGCGC AATGACAAGC CATTGAGTGC GGGTCCTGCT
    CGGACAGGGC CTATTTCATT GAGCAGTAGC AATAAGGAGA CCCCCGAAGA GCTGGTTCAG
    GAAGTGTCCC GCAGAGACTC CAATGCTTCA GATACACCCA AGCTGCTCGT CAGTACTGCA
    GACAAGAGCA GACTGGAGAA CAGCCAACCC AGAGAGTCTG CTGTTAAACT TGAAGCACTC
    TCAGGGCCCT CTCCAGATAA ACCTGCATTA TCAGAAGAGG AGATGGAGAG GCGGTCCAAG
    TCTATAATTG ATGAGTTTTT GCACATTAAT GATTACAAGG AAGCTTTGCA GTGTGTTGAG
    GAACTAGAGC AGTCTGCCAT GCTCTATGTG TTTGTGCGGG TGGGTGTGGA GTCCACGCTG
    GAAAGGAGTC AGATCACGCG AGACCATATG GGTCAGCTGC TCTTCCAGCT TCTGCAGGCT
    GGAGTCCTCC CTAAACTTCA GTTCTTTAAA GGGTTTTCTG AAACTCTTGA ATTAGCAGAT
    GATATGGCCA TTGATATTCC CCATATATGG CTGTACCTTG CGGAGCTGGT AACCCCTGTG
    CTTCGAGAGG GGGGAATCTC TATGAGAGAA TTATTTAGTG AATTTAGCAA ACCATTACTC
    CCTGTGGGAA GAGCTGGGAT ATTATTTTCT GAAATATTGC ACCTTCTATG CAAACAAATG
    AGCCATAAGA AAGTGGGATC ATTATGGAGG GATTCTGGGT TGAGCTGGGC AGATTTTATA
    CCTGAAACAG AGGATGTGCA CAGCTTCATC ACAGAACATA AACTAGAGTT CACTCTGTCT
    GATGGCTCAA ACCCGACCGA GGCTGTCTCT AAGAGAACAC TCTCGCCTGA GGAACTGAAC
    AAGCAACTTG AAAAACTTTT ACTTGAGGAC ATGGTTGGCG ATGAACAAAT CTTCGACTGG
    GTTGAGGCCA ATCTAGATGA ATCAGAAATG AGTTCAGCTC CTTTCGTCAG AGCTTTAATG
    ACTGCTGTGT GTAAAGCAGC AGTAAAAACT GAGGGCTCCT CGTGTAAAGT GGACTTGTCC
    ATAATTCAGA CGAGATTGCC TGTATTACAC AAGTACCTTA ACTCAGACAC AGAGAGACAG
    TTGCAAGCAC TTTATGCACT TCAAGCATTG ATAGTAAAAC TGGACCAGCC TGCTAATTTG
    CTCCGGATGT TTTTTGACTG TTTGTATGAT GAGGACGTGA TCTCTGAAGA CGCTTTCTAT
    AAATGGGAGG TCAGTAAAGA CCCCGCTGAA CAGCAAGGCA AAGGTGTTGC CCTGAAGTCC
    GTCACAGCCT TCTTCACGTG GCTGCGCGAG GCCGAGGAAG AGTCCGAGGA CAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295176.1
    CDS605..5701
    Translation

    Target ORF information:

    RefSeq Version XM_009296901.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296901.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACTAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CAACAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTTTCCAG AGGACGCAGA TGCAAACGGC CCGGCCCACC
    ATTCCAAGCA ACACCCCACC CATTCGGCCC ACGTCTCAGA CCCCCACGGC AGCAGTATAT
    TCTCCCAATC AGCACATCAT GATGACCATG GCTCACATGC CCTTTCATTC CCCACAGACA
    GCGCAGTACT ACATCCCTCA GTATCGTCAC AGTGCTCCAC AGTATGTGGG ACCTCCTCAA
    CAGTACCCTG TCCAGCCGAC TGGACCCAGT ACCTTCTATG CTGCAGCCAG TCCAGGGGAG
    TTTCCTGCAC CTTATGCAGC AGGACCTCCC TACTATCCTG GGCAGCCCGT GTACACACCA
    TCTCCACCCA TTATAGTGCC TACACCACAA CAACCTCCAC CTGCTAAGAG GGAAAAGAAA
    ACAATCCGCA TCCGAGACCC TAACCAGGGT GGCAAGGATG TCACAGACGA AATATTATCA
    GGAGTGGGTT TGAGTCGAAA CCCAACCCCG CCTGTGGGAC GGCCCTCTTC CACTCCTACA
    CCTCCACAGC AGCTGAACAG CCAGGTAGCA GATCATGGGC ACATCATGTA TAATGTGGAC
    AGCAGCCCGC ACCTTCCAGC ATCCTTCAAC TTGAAAGCAG ACGATAAGCC AAAACTAGAG
    TTTTCCTTGC AAAGAACTGC ATCTCCTGGT CTGCGGCAGC CCGACACTCC TCTGGAGAGA
    AGAGATCCCA GCAGCCCAGT CCAAACACCC TCCTCTCCCC CACACAAACC TGAGCTTCCC
    CCCTCCGACT CAGAGACGGC CTCAAGTGTA GCTACAGCCC CGACTCCTTC CATACCTGCC
    TCAACAGAAG AGTCAGCTGA TGCCCCGAGC CCATTAGCTG AGCCCAGTCT CACTAAAGCT
    ATCACACCTG AACCAGAGAG CTCTGAGCCA GAGAAATCCT CCTCACCACC ACCTCAAAGT
    CTGTCTGGAT CCCTTACCCA GCACGAAAAA GCCGTCAATG GGCTCACGGA TGTTGACGCT
    GCTCCACTGA GTGAAGAATT GGAAACCCAA CCCAGGGAAG CCTCTCCATT GCTGCCTACT
    TCCAGTGTGC CCCAGTCAGA GCCCAGGCCA GTCACACCGG TGCTGGAGGA AGAGTCTGAT
    CCAATCAATA TGAACAGTCC TTTGCCTCCT GTTGAGGACG ATGCTGGATG TCCTGACAAC
    GTTTCCCCCT CCCTCTCCAC CTCCACGACT GCTGCTATCT CCACCACTCC TCCTGCTCCT
    CCTCCAGGAC TCTCGCACCC CAGTCAGGTT TCTGCAGCGT TGGACAGGAG ACCCTCTAAT
    GGAGCAGAGA TCAAAGAAAC AGGGAAGGAA AACGAGGCCT TGCCGGACAA GAGAGGAGAG
    CCTTTCTTAC AGTCCAGAAA AAGCTCAAAT CAAGCTACAT CTAGTGCACC AAAGACCTGG
    AAGAAGCCAA AAGAGGACAT GCCTGTAGGG CAAGATCAGT GTCCAGACAA AGAGGAGGAC
    ACTGATCAGG CCAGTATGGA ACCTGCTCCT GAAAGCCCCA GTCCTCCTCC TGTGGAGGTG
    GAGAGGAGGC CATCAGTAGG AGCAGTGCTC GAGGAGAATG GAGAACAGGA AGTGGAGCCT
    TTGAGAAACG GTGCTGAACA CACTTCTGAA ACTGAGAGCA GTGACAGCAT CCACCCGCCT
    GCAGTCAAAG AGCCGTTGAT CAAAGTATTG CCACAGCCTG CAGAGAAGAA TGGAAAGAAG
    CAGTACAACA GAGACTTCCT GCTGGGCTTC CAGTTTATGC CTGCATGTGT GCAGAAACCA
    GAAGGGCTTC CTCCCATTTC TGATGTTGTT CTAGATAAGA TTAACCAAAA TAAGCTGCCC
    ATGCGGCCGG TAGACCCACG TGTGCTTTCC AGAGGCCCGG ACTTTACGCC AGCGTTTGCG
    GATTTCAGCC GACCCATTTC AGGAGGCAGA GGGGCACCGT TGCTGAATGT TGGCATGCGA
    CGGCCACCTG GACGCAAGAT CATCACAAAC GTGTCTGTGA TTGATGAGGT GCAGCTGAAG
    ACAACAGAGA ATGCCTGGAA GCCTGGCATG AAGCGGGAAG GTGCAGTTGA AGATCCAGAT
    GCCCAGAGAA CTCAGGAGCT TTTCCGAAAA GTGCGCAGTA TTCTCAATAA ACTGACACCG
    CAGATGTTCA GCCAGCTGAT GAAGCAGGTG ACTGACCTCA CCATCGACAC AGAGGAGCGA
    CTCAAGGGTG TCATAGATCT GGTCTTTGAA AAAGCAATCA ATGAGCCCAG CTTCTCTGTG
    GCCTACGGCA ACATGTGCAG CTGTCTGGCC ACGTTAAAAG TGCCCATGAC GGATAAGCCC
    AACAGCACTG TGAATTTCCG GAAGCTGCTT CTGAACCGCT GTCAGAAAGA GTTTGAGAAG
    GACAAGATGG ATGACGATGC CTTTGAGAAG AAACACAGGG AGCTGGAGGC AGCTACTGCG
    TCCAGTGAGC GGGAGAGGCT TCAGGAGGAG CTGGAAGAGG CCAAAGACAA GGCCCGAAGA
    CGCTCCATTG GCAACATTAA GTTCATCGGC GAGCTCTTCA AGCTGCGGAT GCTGACAGAG
    GCCATCATGC ATGACTGCGT GGTGAAGTTG CTGAAAAACC ATGATGAGGA ATCACTGGAG
    TGCCTGTGTA GACTGCTCAC CACTATTGGA AAGGACCTGG ACTTTGAGAA AGCTAAGCCA
    CGAATGGATC AGTACTTTAA TCAGATGGAG AAGATAGTTA AGGAGAGGAA GACTTCTTCA
    CGCATTCGCT TCATGCTGCA GGATGTTATT GATCTGCGAT TGCACAACTG GGTTTCTCGA
    AGAGCAGATC AGGGCCCCAA GACCATCGAG CAGATCCATA AAGACGCAAA GCTGGAGGAG
    CAGGAGGAAC AGAGGAAAGT CCACCAGCAA CTGCTTTCCA AAGACAACAA GAGGAGACCA
    GTGGTGCAGA GAGAGGAGAC CTGGAGCACT GTGCCTATGA CTAAGAACAG CAGAACAATA
    GACCCCGCCA AGATTCCCAA ATTCTCCAAG CCAGCGATAG ATGAGAAGAT TCAGCTGGGG
    CCGAGAGCTC AGGTGAACTG GATGAAGGGC AGCAGTGGTG GAGCTGGAGC AAAGGCCAGT
    GAGTCAGATG CGTCTCGGCC GAGTGCCAGT TTGAATCGTT ACTCTCCTCT GCAACCCTCG
    GCACTCCAGA CTTCTTCTTT ACCCTCCACA TCTCCAGATT TTGATTCCAG GAGAGTTCTC
    GGCAGTCGTG GAAGCTCAGG ACGGGAGCGC AATGACAAGC CATTGAGTGC GGGTCCTGCT
    CGGACAGGGC CTATTTCATT GAGCAGTAGC AATAAGGAGA CCCCCGAAGA GCTGGTTCAG
    GAAGTGTCCC GCAGAGACTC CAATGCTTCA GATACACCCA AGCTGCTCGT CAGTACTGCA
    GACAAGAGCA GACTGGAGAA CAGCCAACCC AGAGAGTCTG CTGTTAAACT TGAAGCACTC
    TCAGGGCCCT CTCCAGATAA ACCTGCATTA TCAGAAGAGG AGATGGAGAG GCGGTCCAAG
    TCTATAATTG ATGAGTTTTT GCACATTAAT GATTACAAGG AAGCTTTGCA GTGTGTTGAG
    GAACTAGAGC AGTCTGCCAT GCTCTATGTG TTTGTGCGGG TGGGTGTGGA GTCCACGCTG
    GAAAGGAGTC AGATCACGCG AGACCATATG GGTCAGCTGC TCTTCCAGCT TCTGCAGGCT
    GGAGTCCTCC CTAAACTTCA GTTCTTTAAA GGGTTTTCTG AAACTCTTGA ATTAGCAGAT
    GATATGGCCA TTGATATTCC CCATATATGG CTGTACCTTG CGGAGCTGGT AACCCCTGTG
    CTTCGAGAGG GGGGAATCTC TATGAGAGAA TTATTTAGTG AATTTAGCAA ACCATTACTC
    CCTGTGGGAA GAGCTGGGAT ATTATTTTCT GAAATATTGC ACCTTCTATG CAAACAAATG
    AGCCATAAGA AAGTGGGATC ATTATGGAGG GATTCTGGGT TGAGCTGGGC AGATTTTATA
    CCTGAAACAG AGGATGTGCA CAGCTTCATC ACAGAACATA AACTAGAGTT CACTCTGTCT
    GATGGCTCAA ACCCGACCGA GGCTGTCTCT AAGAGAACAC TCTCGCCTGA GGAACTGAAC
    AAGCAACTTG AAAAACTTTT ACTTGAGGAC ATGGTTGGCG ATGAACAAAT CTTCGACTGG
    GTTGAGGCCA ATCTAGATGA ATCAGAAATG AGTTCAGCTC CTTTCGTCAG AGCTTTAATG
    ACTGCTGTGT GTAAAGCAGC AGTAAAAACT GAGGGCTCCT CGTGTAAAGT GGACTTGTCC
    ATAATTCAGA CGAGATTGCC TGTATTACAC AAGTACCTTA ACTCAGACAC AGAGAGACAG
    TTGCAAGCAC TTTATGCACT TCAAGCATTG ATAGTAAAAC TGGACCAGCC TGCTAATTTG
    CTCCGGATGT TTTTTGACTG TTTGTATGAT GAGGACGTGA TCTCTGAAGA CGCTTTCTAT
    AAATGGGAGG TCAGTAAAGA CCCCGCTGAA CAGCAAGGCA AAGGTGTTGC CCTGAAGTCC
    GTCACAGCCT TCTTCACGTG GCTGCGCGAG GCCGAGGAAG AGTCCGAGGA CAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146816
    Clone ID Related Accession (Same CDS sequence) XM_021474541.1
    Accession Version XM_021474541.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5202bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395362.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACCAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CACCAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTGCCCCT GGGCCTCGTC CTACCCATCA CCAGGGAGGA
    TTCAGATCCA TTCAGTTTTT CCAGAGGACG CAGATGCAAA CGGCCCGGCC CACCATTCCA
    AGCAACACCC CACCCATTCG GCCCACGTCT CAGACCCCCA CGGCAGCAGT ATATTCTCCC
    AATCAGCACA TCATGATGAC CATGGCTCAC ATGCCCTTTC ATTCCCCACA GACAGCGCAG
    TACTACATCC CTCAGTATCG TCACAGTGCT CCACAGTATG TGGGACCTCC TCAACAGTAC
    CCTGTCCAGC CCACTGGACC CAGTACCTTC TATGCTGCAG CCAGTCCAGG GGAGTTTCCT
    GCACCTTATG CAGCAGGACC TCCCTACTAT CCTGGGCAGC CCGTGTACAC ACCATCTCCA
    CCCATTATAG TGCCTACACC ACAACAACCT CCACCTGCTA AGAGGGAAAA GAAAACAATC
    CGCATCCGAG ACCCTAACCA GGGTGGCAAG GATGTCACAG ACGAAATATT ATCAGGAGTG
    GGTTTGAGTC GAAACCCAAC CCCGCCTGTG GGACGGCCCT CTTCCACTCC TACACCTCCA
    CAGTTTTTAT GTCCACACCC TCATTATCCT CACATTTTCT ACCTCAAATC CCAGCAGCTG
    AACAGCCAGG TAGCAGATCA TGGGCACATC ATGTATAATG TGGACAGCAG CCCGCACCTT
    CCTGCACCCT TCAACTTGAA AGCAGATGAT AAGCCAAAAC TAGAGTTTTC CTTGCAAAGA
    ACTGCATCTC CTGGTCTGCG GCAGCCCGAC ACTCCTCTGG AGAGAAGAGA TCCCAGCAGC
    CCAGTCCAAA CACCCTCCTC TCCCCCACAC AAACCTGAGC TTCCCCCCTC CGACTCAGAG
    ACGGCCTCAA GTGTAGCTAC AGCCCCGACT CCTTCCATAC CTGCCTCAAC AGAAGAGTCA
    GCTGATGCCC CGAGCCCATT AGCTGAGCCC AGTCTCACTA AAGCTATCAC ACCTGAACCA
    GAGAGCTCTG AGCCAGAGAA ATCCTCCTCA CCACCACCTC AAAGTCTGTC TGGATCCCTT
    ACCCAGCACG AAAAAGCCGT CAATGGGCTC ACGGACGTTG ACGCTGCTCC ACTGAGTGAA
    GAATTGGAAA CCCAACCCAG GGAAGCCTCT CCATTGCTGC CTACTTCCAG TGTGCCCCAG
    TCAGAGCCCA GGCCAGTCAC ACCGGTGCTG GAGGAAGAGT CTGATCCAAT CAATATGGAC
    AGTCCTTTGC CTCCTGTTGA AGACGATGCT GGATGTCCTG ACAACGTTTC CCCCTCCCTC
    TCCACCTCCA CGACTGCTGC TATCTCCACC ACTCCTCCTG CTCCTCCTCC AGGACTCTCG
    CACCCCAGTC AGGTTTCTGC AGCGTTGGAC AGGAGACCCT CTAATGGAGC AGAGATCAAA
    GAAACAGGGA AGGAAAACGA GGCCTTGCCG GACAAGAGAG GAGAGCCTTT CTTACAGTCC
    AGAAAAAGCT CAAATCAAGC TACATCTAGT GCACCAAAGA CCTGGAAGAA GCCAAAAGAG
    GACATGCCTG TAGGGCAAGA TCAGTGTCCA GATAAAGAGG AGGACATTGA TCAGACCAGT
    ATGGAACCTG CTCCTGAAAG CCCCAGTCCT CCTCCTGTGG AGGTGGAGAG GAGGCCATCA
    GTAGGAGCAG TGCTCGAGGA GAATGGAGAA CAGGAAGTGG AGCCTTTGAG AAACGGTGCT
    GAACACACTT CTGAAACTGA GAGCAGTGAC AGCATCCACC CGCCTGCAGT CAAAGAGCCG
    TTGATCAAAG TATTGCCACA GCCTGCAGAG AAGAATGGAA AGAAGCAGTA CAACAGAGAC
    TTCCTGCTGG GCTTCCAGTT TATGCCTGCA TGTGTGCAGA AACCAGAAGG GCTTCCTCCC
    ATTTCTGATG TTGTTCTAGA TAAGATTAAC CAAAATAAGC TGCCCATGCG GCCGGTAGAC
    CCACGTGTGC TTTCCAGAGG CCCGGATTTT ACGCCAGCAT TTGCGGATTT CAGCCGACCC
    ATTTCAGGAG GCAGAGGGGC ACCGTTGCTG AATGTTGGCA TGCGACGGCC ACCTGGACGC
    AAGATCATCA CAAACGTGTC TGTGATTGAT GAGGTGCAGC TGAAGACAAC AGAGAATGCC
    TGGAAGCCTG GCATGAAGCG GGAAGGTGCA GTTGAAGATC CAGATGCCCA GAGAACTCAG
    GAGCTTTTCC GAAAAGTGCG CAGTATTCTC AATAAACTGA CACCGCAGAT GTTCAGCCAG
    CTGATGAAGC AGGTGACTGA CCTCACCATC GACACAGAGG AGCGACTCAA GGGTGTCATA
    GATCTGGTCT TTGAAAAAGC AATCAATGAG CCCAGCTTCT CTGTGGCCTA CGGCAACATG
    TGCAGCTGTC TGGCCACGTT AAAAGTGCCC ATGACGGATA AGCCCAACAG CACTGTGAAT
    TTCCGGAAGC TGCTTCTGAA CCGCTGTCAG AAAGAGTTTG AGAAGGACAA GATGGATGAC
    GATGCCTTCG AGAAGAAACA CAGGGAGCTG GAGGCAGCTA CTGCGTCCAG TGAGCGGGAG
    AGGCTTCAGG AGGAGCTGGA AGAGGCCAAA GACAAGGCCC GAAGACGCTC CATTGGCAAC
    ATTAAGTTCA TCGGCGAGCT CTTCAAGCTG CGGATGCTGA CAGAGGCCAT CATGCATGAC
    TGCGTGGTGA AGTTGCTGAA AAACCATGAT GAGGAATCAC TGGAGTGCCT GTGTAGACTG
    CTCACCACTA TTGGAAAGGA CCTGGACTTT GAGAAAGCTA AGCCACGAAT GGATCAGTAC
    TTTAATCAGA TGGAGAAGAT AGTTAAGGAG AGGAAGACTT CTTCACGCAT TCGCTTCATG
    CTGCAGGATG TTATTGATCT GCGATTGCAC AACTGGGTTT CTCGAAGAGC AGATCAGGGC
    CCCAAGACCA TCGAGCAGAT CCATAAAGAC GCAAAGCTGG AGGAGCAGGA GGAACAGAGG
    AAAGTCCACC AGCAACTGCT TTCCAAAGAC AACAAGAGGA GACCAGTGGT GCAGAGAGAG
    GAGACCTGGA GCACTGTGCC TATGACTAAG AACAGCAGAA CAATAGACCC CGCCAAGATT
    CCCAAATTCT CCAAGTCAGC GATAGATGAG AAGATTCAGC TGGGGCCGAG AGCTCAGGTG
    AACTGGATGA AGGGCAGCAG TGGTGGAGCT GGAGCAAAGG CCAGTGAGTC AGATGCGTCT
    CGGCCGAGTG CCAGTTTGAA TCGTTACTCT CCTCTGCAAC CCTCGGCACT CCAGACTTCT
    TCTTTACCCT CCACATCTCC AGATTTTGAT TCCAGGAGAG TTCTCGGCAG TCGTGGAAGC
    TCAGGACGGG AGCGCAATGA CAAGCCATTG AGTGCGGGTC CTGCTCGGAC AGGGCCTATT
    TCATTGAGCA GTAGCAATAA GGAGACCCCC GAAGAGCTGG TTCAGGAAGT GTCCCGCAGA
    GACTCCAATG CTTCAGATAC ACCCAAGCTG CTCGTCAGTA CTGCAGACAA GAGCAGACTG
    GAGAACAGCC AACCCAGAGA GTCTGCTGTT AAACTTGAAG CACTCTCAGG ACCCTCTCCA
    GATAAACCTG CATTATCAGA AGAGGAGATG GAGAGGCGGT CCAAGTCTAT AATTGATGAG
    TTTTTGCACA TTAATGATTA CAAGGAAGCT TTGCAGTGTG TTGAGGAACT AGAGCAGTCT
    GCCATGCTCT ATGTGTTTGT GCGGGTGGGT GTGGAGTCCA CGCTGGAAAG GAGTCAGATC
    ACGCGAGACC ATATGGGTCA GCTGCTCTTC CAGCTTCTGC AGGCTGGAGT CCTCCTTAAA
    CTTCAGTTCT TTAAAGGGTT TTCTGAAACT CTTGAATTAG CAGATGATAT GGCCATTGAT
    ATTCCCCATA TATGGCTGTA CCTTGCGGAG CTGGTAACCC CTGTGCTTCG AGAGGGGGGA
    ATCTCTATGA GAGAATTATT TAGTGAATTT AGCAAACCAT TACTCCCTGT GGGAAGAGCT
    GGGATATTAT TTTCTGAAAT ATTGCACCTT CTATGCAAAC AAATGAGCCA TAAGAAAGTG
    GGATCATTAT GGAGGGATTC TGGTTTGAGC TGGGCAGATT TTATACCTGA AACAGAGGAT
    GTGCACAGCT TCATCACAGA ACATAAACTA GAGTTCACTC TGTCTGATGG CTCAAACCCG
    ACCGAGGCTG TCTCTAAGAG AACACTCTCG CCTGAGGAAC TGAACAAGCA ACTTGAAAAA
    CTTTTACTTG AGGACATGGT TGGCGATGAA CAAATCTTTG ACTGGGTTGA GGCCAATCTA
    GATGAATCAG AAATGAGTTC AGCTCCTTTC GTCAGAGCTT TAATGACTGC TGTGTGTAAA
    GCAGCAGTAA AAACTGAGGG CTCCTCGTGT AAAGTGGACT TGTCCATAAT TCAGACGAGA
    TTGCCTGTAT TACACAAGTA CCTTAACTCA GACACAGAGA GACAGTTGCA AGCACTTTAT
    GCACTTCAAG CATTGATAGT AAAACTGGAC CAGCCTGCTA ATTTGCTCCG GATGTTTTTT
    GACTGTTTGT ATGATGAGGA CGTGATCTCT GAAGACGCTT TCTATAAATG GGAGGTCAGT
    AAAGACCCCG CTGAACAGCA AGGCAAAGGT GTTGCCCTGA AGTCCGTCAC AGCCTTCTTC
    ACGTGGCTGC GCGAGGCCGA GGAAGAGTCC GAGGACAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330216.1
    CDS528..5729
    Translation

    Target ORF information:

    RefSeq Version XM_021474541.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474541.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACCAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CACCAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTGCCCCT GGGCCTCGTC CTACCCATCA CCAGGGAGGA
    TTCAGATCCA TTCAGTTTTT CCAGAGGACG CAGATGCAAA CGGCCCGGCC CACCATTCCA
    AGCAACACCC CACCCATTCG GCCCACGTCT CAGACCCCCA CGGCAGCAGT ATATTCTCCC
    AATCAGCACA TCATGATGAC CATGGCTCAC ATGCCCTTTC ATTCCCCACA GACAGCGCAG
    TACTACATCC CTCAGTATCG TCACAGTGCT CCACAGTATG TGGGACCTCC TCAACAGTAC
    CCTGTCCAGC CCACTGGACC CAGTACCTTC TATGCTGCAG CCAGTCCAGG GGAGTTTCCT
    GCACCTTATG CAGCAGGACC TCCCTACTAT CCTGGGCAGC CCGTGTACAC ACCATCTCCA
    CCCATTATAG TGCCTACACC ACAACAACCT CCACCTGCTA AGAGGGAAAA GAAAACAATC
    CGCATCCGAG ACCCTAACCA GGGTGGCAAG GATGTCACAG ACGAAATATT ATCAGGAGTG
    GGTTTGAGTC GAAACCCAAC CCCGCCTGTG GGACGGCCCT CTTCCACTCC TACACCTCCA
    CAGTTTTTAT GTCCACACCC TCATTATCCT CACATTTTCT ACCTCAAATC CCAGCAGCTG
    AACAGCCAGG TAGCAGATCA TGGGCACATC ATGTATAATG TGGACAGCAG CCCGCACCTT
    CCTGCACCCT TCAACTTGAA AGCAGATGAT AAGCCAAAAC TAGAGTTTTC CTTGCAAAGA
    ACTGCATCTC CTGGTCTGCG GCAGCCCGAC ACTCCTCTGG AGAGAAGAGA TCCCAGCAGC
    CCAGTCCAAA CACCCTCCTC TCCCCCACAC AAACCTGAGC TTCCCCCCTC CGACTCAGAG
    ACGGCCTCAA GTGTAGCTAC AGCCCCGACT CCTTCCATAC CTGCCTCAAC AGAAGAGTCA
    GCTGATGCCC CGAGCCCATT AGCTGAGCCC AGTCTCACTA AAGCTATCAC ACCTGAACCA
    GAGAGCTCTG AGCCAGAGAA ATCCTCCTCA CCACCACCTC AAAGTCTGTC TGGATCCCTT
    ACCCAGCACG AAAAAGCCGT CAATGGGCTC ACGGACGTTG ACGCTGCTCC ACTGAGTGAA
    GAATTGGAAA CCCAACCCAG GGAAGCCTCT CCATTGCTGC CTACTTCCAG TGTGCCCCAG
    TCAGAGCCCA GGCCAGTCAC ACCGGTGCTG GAGGAAGAGT CTGATCCAAT CAATATGGAC
    AGTCCTTTGC CTCCTGTTGA AGACGATGCT GGATGTCCTG ACAACGTTTC CCCCTCCCTC
    TCCACCTCCA CGACTGCTGC TATCTCCACC ACTCCTCCTG CTCCTCCTCC AGGACTCTCG
    CACCCCAGTC AGGTTTCTGC AGCGTTGGAC AGGAGACCCT CTAATGGAGC AGAGATCAAA
    GAAACAGGGA AGGAAAACGA GGCCTTGCCG GACAAGAGAG GAGAGCCTTT CTTACAGTCC
    AGAAAAAGCT CAAATCAAGC TACATCTAGT GCACCAAAGA CCTGGAAGAA GCCAAAAGAG
    GACATGCCTG TAGGGCAAGA TCAGTGTCCA GATAAAGAGG AGGACATTGA TCAGACCAGT
    ATGGAACCTG CTCCTGAAAG CCCCAGTCCT CCTCCTGTGG AGGTGGAGAG GAGGCCATCA
    GTAGGAGCAG TGCTCGAGGA GAATGGAGAA CAGGAAGTGG AGCCTTTGAG AAACGGTGCT
    GAACACACTT CTGAAACTGA GAGCAGTGAC AGCATCCACC CGCCTGCAGT CAAAGAGCCG
    TTGATCAAAG TATTGCCACA GCCTGCAGAG AAGAATGGAA AGAAGCAGTA CAACAGAGAC
    TTCCTGCTGG GCTTCCAGTT TATGCCTGCA TGTGTGCAGA AACCAGAAGG GCTTCCTCCC
    ATTTCTGATG TTGTTCTAGA TAAGATTAAC CAAAATAAGC TGCCCATGCG GCCGGTAGAC
    CCACGTGTGC TTTCCAGAGG CCCGGATTTT ACGCCAGCAT TTGCGGATTT CAGCCGACCC
    ATTTCAGGAG GCAGAGGGGC ACCGTTGCTG AATGTTGGCA TGCGACGGCC ACCTGGACGC
    AAGATCATCA CAAACGTGTC TGTGATTGAT GAGGTGCAGC TGAAGACAAC AGAGAATGCC
    TGGAAGCCTG GCATGAAGCG GGAAGGTGCA GTTGAAGATC CAGATGCCCA GAGAACTCAG
    GAGCTTTTCC GAAAAGTGCG CAGTATTCTC AATAAACTGA CACCGCAGAT GTTCAGCCAG
    CTGATGAAGC AGGTGACTGA CCTCACCATC GACACAGAGG AGCGACTCAA GGGTGTCATA
    GATCTGGTCT TTGAAAAAGC AATCAATGAG CCCAGCTTCT CTGTGGCCTA CGGCAACATG
    TGCAGCTGTC TGGCCACGTT AAAAGTGCCC ATGACGGATA AGCCCAACAG CACTGTGAAT
    TTCCGGAAGC TGCTTCTGAA CCGCTGTCAG AAAGAGTTTG AGAAGGACAA GATGGATGAC
    GATGCCTTCG AGAAGAAACA CAGGGAGCTG GAGGCAGCTA CTGCGTCCAG TGAGCGGGAG
    AGGCTTCAGG AGGAGCTGGA AGAGGCCAAA GACAAGGCCC GAAGACGCTC CATTGGCAAC
    ATTAAGTTCA TCGGCGAGCT CTTCAAGCTG CGGATGCTGA CAGAGGCCAT CATGCATGAC
    TGCGTGGTGA AGTTGCTGAA AAACCATGAT GAGGAATCAC TGGAGTGCCT GTGTAGACTG
    CTCACCACTA TTGGAAAGGA CCTGGACTTT GAGAAAGCTA AGCCACGAAT GGATCAGTAC
    TTTAATCAGA TGGAGAAGAT AGTTAAGGAG AGGAAGACTT CTTCACGCAT TCGCTTCATG
    CTGCAGGATG TTATTGATCT GCGATTGCAC AACTGGGTTT CTCGAAGAGC AGATCAGGGC
    CCCAAGACCA TCGAGCAGAT CCATAAAGAC GCAAAGCTGG AGGAGCAGGA GGAACAGAGG
    AAAGTCCACC AGCAACTGCT TTCCAAAGAC AACAAGAGGA GACCAGTGGT GCAGAGAGAG
    GAGACCTGGA GCACTGTGCC TATGACTAAG AACAGCAGAA CAATAGACCC CGCCAAGATT
    CCCAAATTCT CCAAGTCAGC GATAGATGAG AAGATTCAGC TGGGGCCGAG AGCTCAGGTG
    AACTGGATGA AGGGCAGCAG TGGTGGAGCT GGAGCAAAGG CCAGTGAGTC AGATGCGTCT
    CGGCCGAGTG CCAGTTTGAA TCGTTACTCT CCTCTGCAAC CCTCGGCACT CCAGACTTCT
    TCTTTACCCT CCACATCTCC AGATTTTGAT TCCAGGAGAG TTCTCGGCAG TCGTGGAAGC
    TCAGGACGGG AGCGCAATGA CAAGCCATTG AGTGCGGGTC CTGCTCGGAC AGGGCCTATT
    TCATTGAGCA GTAGCAATAA GGAGACCCCC GAAGAGCTGG TTCAGGAAGT GTCCCGCAGA
    GACTCCAATG CTTCAGATAC ACCCAAGCTG CTCGTCAGTA CTGCAGACAA GAGCAGACTG
    GAGAACAGCC AACCCAGAGA GTCTGCTGTT AAACTTGAAG CACTCTCAGG ACCCTCTCCA
    GATAAACCTG CATTATCAGA AGAGGAGATG GAGAGGCGGT CCAAGTCTAT AATTGATGAG
    TTTTTGCACA TTAATGATTA CAAGGAAGCT TTGCAGTGTG TTGAGGAACT AGAGCAGTCT
    GCCATGCTCT ATGTGTTTGT GCGGGTGGGT GTGGAGTCCA CGCTGGAAAG GAGTCAGATC
    ACGCGAGACC ATATGGGTCA GCTGCTCTTC CAGCTTCTGC AGGCTGGAGT CCTCCTTAAA
    CTTCAGTTCT TTAAAGGGTT TTCTGAAACT CTTGAATTAG CAGATGATAT GGCCATTGAT
    ATTCCCCATA TATGGCTGTA CCTTGCGGAG CTGGTAACCC CTGTGCTTCG AGAGGGGGGA
    ATCTCTATGA GAGAATTATT TAGTGAATTT AGCAAACCAT TACTCCCTGT GGGAAGAGCT
    GGGATATTAT TTTCTGAAAT ATTGCACCTT CTATGCAAAC AAATGAGCCA TAAGAAAGTG
    GGATCATTAT GGAGGGATTC TGGTTTGAGC TGGGCAGATT TTATACCTGA AACAGAGGAT
    GTGCACAGCT TCATCACAGA ACATAAACTA GAGTTCACTC TGTCTGATGG CTCAAACCCG
    ACCGAGGCTG TCTCTAAGAG AACACTCTCG CCTGAGGAAC TGAACAAGCA ACTTGAAAAA
    CTTTTACTTG AGGACATGGT TGGCGATGAA CAAATCTTTG ACTGGGTTGA GGCCAATCTA
    GATGAATCAG AAATGAGTTC AGCTCCTTTC GTCAGAGCTT TAATGACTGC TGTGTGTAAA
    GCAGCAGTAA AAACTGAGGG CTCCTCGTGT AAAGTGGACT TGTCCATAAT TCAGACGAGA
    TTGCCTGTAT TACACAAGTA CCTTAACTCA GACACAGAGA GACAGTTGCA AGCACTTTAT
    GCACTTCAAG CATTGATAGT AAAACTGGAC CAGCCTGCTA ATTTGCTCCG GATGTTTTTT
    GACTGTTTGT ATGATGAGGA CGTGATCTCT GAAGACGCTT TCTATAAATG GGAGGTCAGT
    AAAGACCCCG CTGAACAGCA AGGCAAAGGT GTTGCCCTGA AGTCCGTCAC AGCCTTCTTC
    ACGTGGCTGC GCGAGGCCGA GGAAGAGTCC GAGGACAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146817
    Clone ID Related Accession (Same CDS sequence) XM_021474542.1
    Accession Version XM_021474542.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5199bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395362.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACCAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CACCAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTGCCCCT GGGCCTCGTC CTACCCATCA CCAGGGAGGA
    TTCAGATCCA TTCAGTTTTT CCAGAGGACG CAGATGCAAA CGGCCCGGCC CACCATTCCA
    AGCAACACCC CACCCATTCG GCCCACGTCT CAGACCCCCA CGGCAGCAGT ATATTCTCCC
    AATCAGCACA TCATGATGAC CATGGCTCAC ATGCCCTTTC ATTCCCCACA GACAGCGCAG
    TACTACATCC CTCAGTATCG TCACAGTGCT CCACAGTATG TGGGACCTCC TCAACAGTAC
    CCTGTCCAGC CCACTGGACC CAGTACCTTC TATGCTGCAG CCAGTCCAGG GGAGTTTCCT
    GCACCTTATG CAGGACCTCC CTACTATCCT GGGCAGCCCG TGTACACACC ATCTCCACCC
    ATTATAGTGC CTACACCACA ACAACCTCCA CCTGCTAAGA GGGAAAAGAA AACAATCCGC
    ATCCGAGACC CTAACCAGGG TGGCAAGGAT GTCACAGACG AAATATTATC AGGAGTGGGT
    TTGAGTCGAA ACCCAACCCC GCCTGTGGGA CGGCCCTCTT CCACTCCTAC ACCTCCACAG
    TTTTTATGTC CACACCCTCA TTATCCTCAC ATTTTCTACC TCAAATCCCA GCAGCTGAAC
    AGCCAGGTAG CAGATCATGG GCACATCATG TATAATGTGG ACAGCAGCCC GCACCTTCCT
    GCACCCTTCA ACTTGAAAGC AGATGATAAG CCAAAACTAG AGTTTTCCTT GCAAAGAACT
    GCATCTCCTG GTCTGCGGCA GCCCGACACT CCTCTGGAGA GAAGAGATCC CAGCAGCCCA
    GTCCAAACAC CCTCCTCTCC CCCACACAAA CCTGAGCTTC CCCCCTCCGA CTCAGAGACG
    GCCTCAAGTG TAGCTACAGC CCCGACTCCT TCCATACCTG CCTCAACAGA AGAGTCAGCT
    GATGCCCCGA GCCCATTAGC TGAGCCCAGT CTCACTAAAG CTATCACACC TGAACCAGAG
    AGCTCTGAGC CAGAGAAATC CTCCTCACCA CCACCTCAAA GTCTGTCTGG ATCCCTTACC
    CAGCACGAAA AAGCCGTCAA TGGGCTCACG GACGTTGACG CTGCTCCACT GAGTGAAGAA
    TTGGAAACCC AACCCAGGGA AGCCTCTCCA TTGCTGCCTA CTTCCAGTGT GCCCCAGTCA
    GAGCCCAGGC CAGTCACACC GGTGCTGGAG GAAGAGTCTG ATCCAATCAA TATGGACAGT
    CCTTTGCCTC CTGTTGAAGA CGATGCTGGA TGTCCTGACA ACGTTTCCCC CTCCCTCTCC
    ACCTCCACGA CTGCTGCTAT CTCCACCACT CCTCCTGCTC CTCCTCCAGG ACTCTCGCAC
    CCCAGTCAGG TTTCTGCAGC GTTGGACAGG AGACCCTCTA ATGGAGCAGA GATCAAAGAA
    ACAGGGAAGG AAAACGAGGC CTTGCCGGAC AAGAGAGGAG AGCCTTTCTT ACAGTCCAGA
    AAAAGCTCAA ATCAAGCTAC ATCTAGTGCA CCAAAGACCT GGAAGAAGCC AAAAGAGGAC
    ATGCCTGTAG GGCAAGATCA GTGTCCAGAT AAAGAGGAGG ACATTGATCA GACCAGTATG
    GAACCTGCTC CTGAAAGCCC CAGTCCTCCT CCTGTGGAGG TGGAGAGGAG GCCATCAGTA
    GGAGCAGTGC TCGAGGAGAA TGGAGAACAG GAAGTGGAGC CTTTGAGAAA CGGTGCTGAA
    CACACTTCTG AAACTGAGAG CAGTGACAGC ATCCACCCGC CTGCAGTCAA AGAGCCGTTG
    ATCAAAGTAT TGCCACAGCC TGCAGAGAAG AATGGAAAGA AGCAGTACAA CAGAGACTTC
    CTGCTGGGCT TCCAGTTTAT GCCTGCATGT GTGCAGAAAC CAGAAGGGCT TCCTCCCATT
    TCTGATGTTG TTCTAGATAA GATTAACCAA AATAAGCTGC CCATGCGGCC GGTAGACCCA
    CGTGTGCTTT CCAGAGGCCC GGATTTTACG CCAGCATTTG CGGATTTCAG CCGACCCATT
    TCAGGAGGCA GAGGGGCACC GTTGCTGAAT GTTGGCATGC GACGGCCACC TGGACGCAAG
    ATCATCACAA ACGTGTCTGT GATTGATGAG GTGCAGCTGA AGACAACAGA GAATGCCTGG
    AAGCCTGGCA TGAAGCGGGA AGGTGCAGTT GAAGATCCAG ATGCCCAGAG AACTCAGGAG
    CTTTTCCGAA AAGTGCGCAG TATTCTCAAT AAACTGACAC CGCAGATGTT CAGCCAGCTG
    ATGAAGCAGG TGACTGACCT CACCATCGAC ACAGAGGAGC GACTCAAGGG TGTCATAGAT
    CTGGTCTTTG AAAAAGCAAT CAATGAGCCC AGCTTCTCTG TGGCCTACGG CAACATGTGC
    AGCTGTCTGG CCACGTTAAA AGTGCCCATG ACGGATAAGC CCAACAGCAC TGTGAATTTC
    CGGAAGCTGC TTCTGAACCG CTGTCAGAAA GAGTTTGAGA AGGACAAGAT GGATGACGAT
    GCCTTCGAGA AGAAACACAG GGAGCTGGAG GCAGCTACTG CGTCCAGTGA GCGGGAGAGG
    CTTCAGGAGG AGCTGGAAGA GGCCAAAGAC AAGGCCCGAA GACGCTCCAT TGGCAACATT
    AAGTTCATCG GCGAGCTCTT CAAGCTGCGG ATGCTGACAG AGGCCATCAT GCATGACTGC
    GTGGTGAAGT TGCTGAAAAA CCATGATGAG GAATCACTGG AGTGCCTGTG TAGACTGCTC
    ACCACTATTG GAAAGGACCT GGACTTTGAG AAAGCTAAGC CACGAATGGA TCAGTACTTT
    AATCAGATGG AGAAGATAGT TAAGGAGAGG AAGACTTCTT CACGCATTCG CTTCATGCTG
    CAGGATGTTA TTGATCTGCG ATTGCACAAC TGGGTTTCTC GAAGAGCAGA TCAGGGCCCC
    AAGACCATCG AGCAGATCCA TAAAGACGCA AAGCTGGAGG AGCAGGAGGA ACAGAGGAAA
    GTCCACCAGC AACTGCTTTC CAAAGACAAC AAGAGGAGAC CAGTGGTGCA GAGAGAGGAG
    ACCTGGAGCA CTGTGCCTAT GACTAAGAAC AGCAGAACAA TAGACCCCGC CAAGATTCCC
    AAATTCTCCA AGTCAGCGAT AGATGAGAAG ATTCAGCTGG GGCCGAGAGC TCAGGTGAAC
    TGGATGAAGG GCAGCAGTGG TGGAGCTGGA GCAAAGGCCA GTGAGTCAGA TGCGTCTCGG
    CCGAGTGCCA GTTTGAATCG TTACTCTCCT CTGCAACCCT CGGCACTCCA GACTTCTTCT
    TTACCCTCCA CATCTCCAGA TTTTGATTCC AGGAGAGTTC TCGGCAGTCG TGGAAGCTCA
    GGACGGGAGC GCAATGACAA GCCATTGAGT GCGGGTCCTG CTCGGACAGG GCCTATTTCA
    TTGAGCAGTA GCAATAAGGA GACCCCCGAA GAGCTGGTTC AGGAAGTGTC CCGCAGAGAC
    TCCAATGCTT CAGATACACC CAAGCTGCTC GTCAGTACTG CAGACAAGAG CAGACTGGAG
    AACAGCCAAC CCAGAGAGTC TGCTGTTAAA CTTGAAGCAC TCTCAGGACC CTCTCCAGAT
    AAACCTGCAT TATCAGAAGA GGAGATGGAG AGGCGGTCCA AGTCTATAAT TGATGAGTTT
    TTGCACATTA ATGATTACAA GGAAGCTTTG CAGTGTGTTG AGGAACTAGA GCAGTCTGCC
    ATGCTCTATG TGTTTGTGCG GGTGGGTGTG GAGTCCACGC TGGAAAGGAG TCAGATCACG
    CGAGACCATA TGGGTCAGCT GCTCTTCCAG CTTCTGCAGG CTGGAGTCCT CCTTAAACTT
    CAGTTCTTTA AAGGGTTTTC TGAAACTCTT GAATTAGCAG ATGATATGGC CATTGATATT
    CCCCATATAT GGCTGTACCT TGCGGAGCTG GTAACCCCTG TGCTTCGAGA GGGGGGAATC
    TCTATGAGAG AATTATTTAG TGAATTTAGC AAACCATTAC TCCCTGTGGG AAGAGCTGGG
    ATATTATTTT CTGAAATATT GCACCTTCTA TGCAAACAAA TGAGCCATAA GAAAGTGGGA
    TCATTATGGA GGGATTCTGG TTTGAGCTGG GCAGATTTTA TACCTGAAAC AGAGGATGTG
    CACAGCTTCA TCACAGAACA TAAACTAGAG TTCACTCTGT CTGATGGCTC AAACCCGACC
    GAGGCTGTCT CTAAGAGAAC ACTCTCGCCT GAGGAACTGA ACAAGCAACT TGAAAAACTT
    TTACTTGAGG ACATGGTTGG CGATGAACAA ATCTTTGACT GGGTTGAGGC CAATCTAGAT
    GAATCAGAAA TGAGTTCAGC TCCTTTCGTC AGAGCTTTAA TGACTGCTGT GTGTAAAGCA
    GCAGTAAAAA CTGAGGGCTC CTCGTGTAAA GTGGACTTGT CCATAATTCA GACGAGATTG
    CCTGTATTAC ACAAGTACCT TAACTCAGAC ACAGAGAGAC AGTTGCAAGC ACTTTATGCA
    CTTCAAGCAT TGATAGTAAA ACTGGACCAG CCTGCTAATT TGCTCCGGAT GTTTTTTGAC
    TGTTTGTATG ATGAGGACGT GATCTCTGAA GACGCTTTCT ATAAATGGGA GGTCAGTAAA
    GACCCCGCTG AACAGCAAGG CAAAGGTGTT GCCCTGAAGT CCGTCACAGC CTTCTTCACG
    TGGCTGCGCG AGGCCGAGGA AGAGTCCGAG GACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330217.1
    CDS528..5726
    Translation

    Target ORF information:

    RefSeq Version XM_021474542.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474542.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACCAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CACCAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTGCCCCT GGGCCTCGTC CTACCCATCA CCAGGGAGGA
    TTCAGATCCA TTCAGTTTTT CCAGAGGACG CAGATGCAAA CGGCCCGGCC CACCATTCCA
    AGCAACACCC CACCCATTCG GCCCACGTCT CAGACCCCCA CGGCAGCAGT ATATTCTCCC
    AATCAGCACA TCATGATGAC CATGGCTCAC ATGCCCTTTC ATTCCCCACA GACAGCGCAG
    TACTACATCC CTCAGTATCG TCACAGTGCT CCACAGTATG TGGGACCTCC TCAACAGTAC
    CCTGTCCAGC CCACTGGACC CAGTACCTTC TATGCTGCAG CCAGTCCAGG GGAGTTTCCT
    GCACCTTATG CAGGACCTCC CTACTATCCT GGGCAGCCCG TGTACACACC ATCTCCACCC
    ATTATAGTGC CTACACCACA ACAACCTCCA CCTGCTAAGA GGGAAAAGAA AACAATCCGC
    ATCCGAGACC CTAACCAGGG TGGCAAGGAT GTCACAGACG AAATATTATC AGGAGTGGGT
    TTGAGTCGAA ACCCAACCCC GCCTGTGGGA CGGCCCTCTT CCACTCCTAC ACCTCCACAG
    TTTTTATGTC CACACCCTCA TTATCCTCAC ATTTTCTACC TCAAATCCCA GCAGCTGAAC
    AGCCAGGTAG CAGATCATGG GCACATCATG TATAATGTGG ACAGCAGCCC GCACCTTCCT
    GCACCCTTCA ACTTGAAAGC AGATGATAAG CCAAAACTAG AGTTTTCCTT GCAAAGAACT
    GCATCTCCTG GTCTGCGGCA GCCCGACACT CCTCTGGAGA GAAGAGATCC CAGCAGCCCA
    GTCCAAACAC CCTCCTCTCC CCCACACAAA CCTGAGCTTC CCCCCTCCGA CTCAGAGACG
    GCCTCAAGTG TAGCTACAGC CCCGACTCCT TCCATACCTG CCTCAACAGA AGAGTCAGCT
    GATGCCCCGA GCCCATTAGC TGAGCCCAGT CTCACTAAAG CTATCACACC TGAACCAGAG
    AGCTCTGAGC CAGAGAAATC CTCCTCACCA CCACCTCAAA GTCTGTCTGG ATCCCTTACC
    CAGCACGAAA AAGCCGTCAA TGGGCTCACG GACGTTGACG CTGCTCCACT GAGTGAAGAA
    TTGGAAACCC AACCCAGGGA AGCCTCTCCA TTGCTGCCTA CTTCCAGTGT GCCCCAGTCA
    GAGCCCAGGC CAGTCACACC GGTGCTGGAG GAAGAGTCTG ATCCAATCAA TATGGACAGT
    CCTTTGCCTC CTGTTGAAGA CGATGCTGGA TGTCCTGACA ACGTTTCCCC CTCCCTCTCC
    ACCTCCACGA CTGCTGCTAT CTCCACCACT CCTCCTGCTC CTCCTCCAGG ACTCTCGCAC
    CCCAGTCAGG TTTCTGCAGC GTTGGACAGG AGACCCTCTA ATGGAGCAGA GATCAAAGAA
    ACAGGGAAGG AAAACGAGGC CTTGCCGGAC AAGAGAGGAG AGCCTTTCTT ACAGTCCAGA
    AAAAGCTCAA ATCAAGCTAC ATCTAGTGCA CCAAAGACCT GGAAGAAGCC AAAAGAGGAC
    ATGCCTGTAG GGCAAGATCA GTGTCCAGAT AAAGAGGAGG ACATTGATCA GACCAGTATG
    GAACCTGCTC CTGAAAGCCC CAGTCCTCCT CCTGTGGAGG TGGAGAGGAG GCCATCAGTA
    GGAGCAGTGC TCGAGGAGAA TGGAGAACAG GAAGTGGAGC CTTTGAGAAA CGGTGCTGAA
    CACACTTCTG AAACTGAGAG CAGTGACAGC ATCCACCCGC CTGCAGTCAA AGAGCCGTTG
    ATCAAAGTAT TGCCACAGCC TGCAGAGAAG AATGGAAAGA AGCAGTACAA CAGAGACTTC
    CTGCTGGGCT TCCAGTTTAT GCCTGCATGT GTGCAGAAAC CAGAAGGGCT TCCTCCCATT
    TCTGATGTTG TTCTAGATAA GATTAACCAA AATAAGCTGC CCATGCGGCC GGTAGACCCA
    CGTGTGCTTT CCAGAGGCCC GGATTTTACG CCAGCATTTG CGGATTTCAG CCGACCCATT
    TCAGGAGGCA GAGGGGCACC GTTGCTGAAT GTTGGCATGC GACGGCCACC TGGACGCAAG
    ATCATCACAA ACGTGTCTGT GATTGATGAG GTGCAGCTGA AGACAACAGA GAATGCCTGG
    AAGCCTGGCA TGAAGCGGGA AGGTGCAGTT GAAGATCCAG ATGCCCAGAG AACTCAGGAG
    CTTTTCCGAA AAGTGCGCAG TATTCTCAAT AAACTGACAC CGCAGATGTT CAGCCAGCTG
    ATGAAGCAGG TGACTGACCT CACCATCGAC ACAGAGGAGC GACTCAAGGG TGTCATAGAT
    CTGGTCTTTG AAAAAGCAAT CAATGAGCCC AGCTTCTCTG TGGCCTACGG CAACATGTGC
    AGCTGTCTGG CCACGTTAAA AGTGCCCATG ACGGATAAGC CCAACAGCAC TGTGAATTTC
    CGGAAGCTGC TTCTGAACCG CTGTCAGAAA GAGTTTGAGA AGGACAAGAT GGATGACGAT
    GCCTTCGAGA AGAAACACAG GGAGCTGGAG GCAGCTACTG CGTCCAGTGA GCGGGAGAGG
    CTTCAGGAGG AGCTGGAAGA GGCCAAAGAC AAGGCCCGAA GACGCTCCAT TGGCAACATT
    AAGTTCATCG GCGAGCTCTT CAAGCTGCGG ATGCTGACAG AGGCCATCAT GCATGACTGC
    GTGGTGAAGT TGCTGAAAAA CCATGATGAG GAATCACTGG AGTGCCTGTG TAGACTGCTC
    ACCACTATTG GAAAGGACCT GGACTTTGAG AAAGCTAAGC CACGAATGGA TCAGTACTTT
    AATCAGATGG AGAAGATAGT TAAGGAGAGG AAGACTTCTT CACGCATTCG CTTCATGCTG
    CAGGATGTTA TTGATCTGCG ATTGCACAAC TGGGTTTCTC GAAGAGCAGA TCAGGGCCCC
    AAGACCATCG AGCAGATCCA TAAAGACGCA AAGCTGGAGG AGCAGGAGGA ACAGAGGAAA
    GTCCACCAGC AACTGCTTTC CAAAGACAAC AAGAGGAGAC CAGTGGTGCA GAGAGAGGAG
    ACCTGGAGCA CTGTGCCTAT GACTAAGAAC AGCAGAACAA TAGACCCCGC CAAGATTCCC
    AAATTCTCCA AGTCAGCGAT AGATGAGAAG ATTCAGCTGG GGCCGAGAGC TCAGGTGAAC
    TGGATGAAGG GCAGCAGTGG TGGAGCTGGA GCAAAGGCCA GTGAGTCAGA TGCGTCTCGG
    CCGAGTGCCA GTTTGAATCG TTACTCTCCT CTGCAACCCT CGGCACTCCA GACTTCTTCT
    TTACCCTCCA CATCTCCAGA TTTTGATTCC AGGAGAGTTC TCGGCAGTCG TGGAAGCTCA
    GGACGGGAGC GCAATGACAA GCCATTGAGT GCGGGTCCTG CTCGGACAGG GCCTATTTCA
    TTGAGCAGTA GCAATAAGGA GACCCCCGAA GAGCTGGTTC AGGAAGTGTC CCGCAGAGAC
    TCCAATGCTT CAGATACACC CAAGCTGCTC GTCAGTACTG CAGACAAGAG CAGACTGGAG
    AACAGCCAAC CCAGAGAGTC TGCTGTTAAA CTTGAAGCAC TCTCAGGACC CTCTCCAGAT
    AAACCTGCAT TATCAGAAGA GGAGATGGAG AGGCGGTCCA AGTCTATAAT TGATGAGTTT
    TTGCACATTA ATGATTACAA GGAAGCTTTG CAGTGTGTTG AGGAACTAGA GCAGTCTGCC
    ATGCTCTATG TGTTTGTGCG GGTGGGTGTG GAGTCCACGC TGGAAAGGAG TCAGATCACG
    CGAGACCATA TGGGTCAGCT GCTCTTCCAG CTTCTGCAGG CTGGAGTCCT CCTTAAACTT
    CAGTTCTTTA AAGGGTTTTC TGAAACTCTT GAATTAGCAG ATGATATGGC CATTGATATT
    CCCCATATAT GGCTGTACCT TGCGGAGCTG GTAACCCCTG TGCTTCGAGA GGGGGGAATC
    TCTATGAGAG AATTATTTAG TGAATTTAGC AAACCATTAC TCCCTGTGGG AAGAGCTGGG
    ATATTATTTT CTGAAATATT GCACCTTCTA TGCAAACAAA TGAGCCATAA GAAAGTGGGA
    TCATTATGGA GGGATTCTGG TTTGAGCTGG GCAGATTTTA TACCTGAAAC AGAGGATGTG
    CACAGCTTCA TCACAGAACA TAAACTAGAG TTCACTCTGT CTGATGGCTC AAACCCGACC
    GAGGCTGTCT CTAAGAGAAC ACTCTCGCCT GAGGAACTGA ACAAGCAACT TGAAAAACTT
    TTACTTGAGG ACATGGTTGG CGATGAACAA ATCTTTGACT GGGTTGAGGC CAATCTAGAT
    GAATCAGAAA TGAGTTCAGC TCCTTTCGTC AGAGCTTTAA TGACTGCTGT GTGTAAAGCA
    GCAGTAAAAA CTGAGGGCTC CTCGTGTAAA GTGGACTTGT CCATAATTCA GACGAGATTG
    CCTGTATTAC ACAAGTACCT TAACTCAGAC ACAGAGAGAC AGTTGCAAGC ACTTTATGCA
    CTTCAAGCAT TGATAGTAAA ACTGGACCAG CCTGCTAATT TGCTCCGGAT GTTTTTTGAC
    TGTTTGTATG ATGAGGACGT GATCTCTGAA GACGCTTTCT ATAAATGGGA GGTCAGTAAA
    GACCCCGCTG AACAGCAAGG CAAAGGTGTT GCCCTGAAGT CCGTCACAGC CTTCTTCACG
    TGGCTGCGCG AGGCCGAGGA AGAGTCCGAG GACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146818
    Clone ID Related Accession (Same CDS sequence) XM_021474543.1
    Accession Version XM_021474543.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5181bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395362.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACCAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CACCAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTGCCCCT GGGCCTCGTC CTACCCATCA CCAGTTTTTC
    CAGAGGACGC AGATGCAAAC GGCCCGGCCC ACCATTCCAA GCAACACCCC ACCCATTCGG
    CCCACGTCTC AGACCCCCAC GGCAGCAGTA TATTCTCCCA ATCAGCACAT CATGATGACC
    ATGGCTCACA TGCCCTTTCA TTCCCCACAG ACAGCGCAGT ACTACATCCC TCAGTATCGT
    CACAGTGCTC CACAGTATGT GGGACCTCCT CAACAGTACC CTGTCCAGCC CACTGGACCC
    AGTACCTTCT ATGCTGCAGC CAGTCCAGGG GAGTTTCCTG CACCTTATGC AGCAGGACCT
    CCCTACTATC CTGGGCAGCC CGTGTACACA CCATCTCCAC CCATTATAGT GCCTACACCA
    CAACAACCTC CACCTGCTAA GAGGGAAAAG AAAACAATCC GCATCCGAGA CCCTAACCAG
    GGTGGCAAGG ATGTCACAGA CGAAATATTA TCAGGAGTGG GTTTGAGTCG AAACCCAACC
    CCGCCTGTGG GACGGCCCTC TTCCACTCCT ACACCTCCAC AGTTTTTATG TCCACACCCT
    CATTATCCTC ACATTTTCTA CCTCAAATCC CAGCAGCTGA ACAGCCAGGT AGCAGATCAT
    GGGCACATCA TGTATAATGT GGACAGCAGC CCGCACCTTC CTGCACCCTT CAACTTGAAA
    GCAGATGATA AGCCAAAACT AGAGTTTTCC TTGCAAAGAA CTGCATCTCC TGGTCTGCGG
    CAGCCCGACA CTCCTCTGGA GAGAAGAGAT CCCAGCAGCC CAGTCCAAAC ACCCTCCTCT
    CCCCCACACA AACCTGAGCT TCCCCCCTCC GACTCAGAGA CGGCCTCAAG TGTAGCTACA
    GCCCCGACTC CTTCCATACC TGCCTCAACA GAAGAGTCAG CTGATGCCCC GAGCCCATTA
    GCTGAGCCCA GTCTCACTAA AGCTATCACA CCTGAACCAG AGAGCTCTGA GCCAGAGAAA
    TCCTCCTCAC CACCACCTCA AAGTCTGTCT GGATCCCTTA CCCAGCACGA AAAAGCCGTC
    AATGGGCTCA CGGACGTTGA CGCTGCTCCA CTGAGTGAAG AATTGGAAAC CCAACCCAGG
    GAAGCCTCTC CATTGCTGCC TACTTCCAGT GTGCCCCAGT CAGAGCCCAG GCCAGTCACA
    CCGGTGCTGG AGGAAGAGTC TGATCCAATC AATATGGACA GTCCTTTGCC TCCTGTTGAA
    GACGATGCTG GATGTCCTGA CAACGTTTCC CCCTCCCTCT CCACCTCCAC GACTGCTGCT
    ATCTCCACCA CTCCTCCTGC TCCTCCTCCA GGACTCTCGC ACCCCAGTCA GGTTTCTGCA
    GCGTTGGACA GGAGACCCTC TAATGGAGCA GAGATCAAAG AAACAGGGAA GGAAAACGAG
    GCCTTGCCGG ACAAGAGAGG AGAGCCTTTC TTACAGTCCA GAAAAAGCTC AAATCAAGCT
    ACATCTAGTG CACCAAAGAC CTGGAAGAAG CCAAAAGAGG ACATGCCTGT AGGGCAAGAT
    CAGTGTCCAG ATAAAGAGGA GGACATTGAT CAGACCAGTA TGGAACCTGC TCCTGAAAGC
    CCCAGTCCTC CTCCTGTGGA GGTGGAGAGG AGGCCATCAG TAGGAGCAGT GCTCGAGGAG
    AATGGAGAAC AGGAAGTGGA GCCTTTGAGA AACGGTGCTG AACACACTTC TGAAACTGAG
    AGCAGTGACA GCATCCACCC GCCTGCAGTC AAAGAGCCGT TGATCAAAGT ATTGCCACAG
    CCTGCAGAGA AGAATGGAAA GAAGCAGTAC AACAGAGACT TCCTGCTGGG CTTCCAGTTT
    ATGCCTGCAT GTGTGCAGAA ACCAGAAGGG CTTCCTCCCA TTTCTGATGT TGTTCTAGAT
    AAGATTAACC AAAATAAGCT GCCCATGCGG CCGGTAGACC CACGTGTGCT TTCCAGAGGC
    CCGGATTTTA CGCCAGCATT TGCGGATTTC AGCCGACCCA TTTCAGGAGG CAGAGGGGCA
    CCGTTGCTGA ATGTTGGCAT GCGACGGCCA CCTGGACGCA AGATCATCAC AAACGTGTCT
    GTGATTGATG AGGTGCAGCT GAAGACAACA GAGAATGCCT GGAAGCCTGG CATGAAGCGG
    GAAGGTGCAG TTGAAGATCC AGATGCCCAG AGAACTCAGG AGCTTTTCCG AAAAGTGCGC
    AGTATTCTCA ATAAACTGAC ACCGCAGATG TTCAGCCAGC TGATGAAGCA GGTGACTGAC
    CTCACCATCG ACACAGAGGA GCGACTCAAG GGTGTCATAG ATCTGGTCTT TGAAAAAGCA
    ATCAATGAGC CCAGCTTCTC TGTGGCCTAC GGCAACATGT GCAGCTGTCT GGCCACGTTA
    AAAGTGCCCA TGACGGATAA GCCCAACAGC ACTGTGAATT TCCGGAAGCT GCTTCTGAAC
    CGCTGTCAGA AAGAGTTTGA GAAGGACAAG ATGGATGACG ATGCCTTCGA GAAGAAACAC
    AGGGAGCTGG AGGCAGCTAC TGCGTCCAGT GAGCGGGAGA GGCTTCAGGA GGAGCTGGAA
    GAGGCCAAAG ACAAGGCCCG AAGACGCTCC ATTGGCAACA TTAAGTTCAT CGGCGAGCTC
    TTCAAGCTGC GGATGCTGAC AGAGGCCATC ATGCATGACT GCGTGGTGAA GTTGCTGAAA
    AACCATGATG AGGAATCACT GGAGTGCCTG TGTAGACTGC TCACCACTAT TGGAAAGGAC
    CTGGACTTTG AGAAAGCTAA GCCACGAATG GATCAGTACT TTAATCAGAT GGAGAAGATA
    GTTAAGGAGA GGAAGACTTC TTCACGCATT CGCTTCATGC TGCAGGATGT TATTGATCTG
    CGATTGCACA ACTGGGTTTC TCGAAGAGCA GATCAGGGCC CCAAGACCAT CGAGCAGATC
    CATAAAGACG CAAAGCTGGA GGAGCAGGAG GAACAGAGGA AAGTCCACCA GCAACTGCTT
    TCCAAAGACA ACAAGAGGAG ACCAGTGGTG CAGAGAGAGG AGACCTGGAG CACTGTGCCT
    ATGACTAAGA ACAGCAGAAC AATAGACCCC GCCAAGATTC CCAAATTCTC CAAGTCAGCG
    ATAGATGAGA AGATTCAGCT GGGGCCGAGA GCTCAGGTGA ACTGGATGAA GGGCAGCAGT
    GGTGGAGCTG GAGCAAAGGC CAGTGAGTCA GATGCGTCTC GGCCGAGTGC CAGTTTGAAT
    CGTTACTCTC CTCTGCAACC CTCGGCACTC CAGACTTCTT CTTTACCCTC CACATCTCCA
    GATTTTGATT CCAGGAGAGT TCTCGGCAGT CGTGGAAGCT CAGGACGGGA GCGCAATGAC
    AAGCCATTGA GTGCGGGTCC TGCTCGGACA GGGCCTATTT CATTGAGCAG TAGCAATAAG
    GAGACCCCCG AAGAGCTGGT TCAGGAAGTG TCCCGCAGAG ACTCCAATGC TTCAGATACA
    CCCAAGCTGC TCGTCAGTAC TGCAGACAAG AGCAGACTGG AGAACAGCCA ACCCAGAGAG
    TCTGCTGTTA AACTTGAAGC ACTCTCAGGA CCCTCTCCAG ATAAACCTGC ATTATCAGAA
    GAGGAGATGG AGAGGCGGTC CAAGTCTATA ATTGATGAGT TTTTGCACAT TAATGATTAC
    AAGGAAGCTT TGCAGTGTGT TGAGGAACTA GAGCAGTCTG CCATGCTCTA TGTGTTTGTG
    CGGGTGGGTG TGGAGTCCAC GCTGGAAAGG AGTCAGATCA CGCGAGACCA TATGGGTCAG
    CTGCTCTTCC AGCTTCTGCA GGCTGGAGTC CTCCTTAAAC TTCAGTTCTT TAAAGGGTTT
    TCTGAAACTC TTGAATTAGC AGATGATATG GCCATTGATA TTCCCCATAT ATGGCTGTAC
    CTTGCGGAGC TGGTAACCCC TGTGCTTCGA GAGGGGGGAA TCTCTATGAG AGAATTATTT
    AGTGAATTTA GCAAACCATT ACTCCCTGTG GGAAGAGCTG GGATATTATT TTCTGAAATA
    TTGCACCTTC TATGCAAACA AATGAGCCAT AAGAAAGTGG GATCATTATG GAGGGATTCT
    GGTTTGAGCT GGGCAGATTT TATACCTGAA ACAGAGGATG TGCACAGCTT CATCACAGAA
    CATAAACTAG AGTTCACTCT GTCTGATGGC TCAAACCCGA CCGAGGCTGT CTCTAAGAGA
    ACACTCTCGC CTGAGGAACT GAACAAGCAA CTTGAAAAAC TTTTACTTGA GGACATGGTT
    GGCGATGAAC AAATCTTTGA CTGGGTTGAG GCCAATCTAG ATGAATCAGA AATGAGTTCA
    GCTCCTTTCG TCAGAGCTTT AATGACTGCT GTGTGTAAAG CAGCAGTAAA AACTGAGGGC
    TCCTCGTGTA AAGTGGACTT GTCCATAATT CAGACGAGAT TGCCTGTATT ACACAAGTAC
    CTTAACTCAG ACACAGAGAG ACAGTTGCAA GCACTTTATG CACTTCAAGC ATTGATAGTA
    AAACTGGACC AGCCTGCTAA TTTGCTCCGG ATGTTTTTTG ACTGTTTGTA TGATGAGGAC
    GTGATCTCTG AAGACGCTTT CTATAAATGG GAGGTCAGTA AAGACCCCGC TGAACAGCAA
    GGCAAAGGTG TTGCCCTGAA GTCCGTCACA GCCTTCTTCA CGTGGCTGCG CGAGGCCGAG
    GAAGAGTCCG AGGACAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330218.1
    CDS528..5708
    Translation

    Target ORF information:

    RefSeq Version XM_021474543.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474543.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACCAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CACCAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTGCCCCT GGGCCTCGTC CTACCCATCA CCAGTTTTTC
    CAGAGGACGC AGATGCAAAC GGCCCGGCCC ACCATTCCAA GCAACACCCC ACCCATTCGG
    CCCACGTCTC AGACCCCCAC GGCAGCAGTA TATTCTCCCA ATCAGCACAT CATGATGACC
    ATGGCTCACA TGCCCTTTCA TTCCCCACAG ACAGCGCAGT ACTACATCCC TCAGTATCGT
    CACAGTGCTC CACAGTATGT GGGACCTCCT CAACAGTACC CTGTCCAGCC CACTGGACCC
    AGTACCTTCT ATGCTGCAGC CAGTCCAGGG GAGTTTCCTG CACCTTATGC AGCAGGACCT
    CCCTACTATC CTGGGCAGCC CGTGTACACA CCATCTCCAC CCATTATAGT GCCTACACCA
    CAACAACCTC CACCTGCTAA GAGGGAAAAG AAAACAATCC GCATCCGAGA CCCTAACCAG
    GGTGGCAAGG ATGTCACAGA CGAAATATTA TCAGGAGTGG GTTTGAGTCG AAACCCAACC
    CCGCCTGTGG GACGGCCCTC TTCCACTCCT ACACCTCCAC AGTTTTTATG TCCACACCCT
    CATTATCCTC ACATTTTCTA CCTCAAATCC CAGCAGCTGA ACAGCCAGGT AGCAGATCAT
    GGGCACATCA TGTATAATGT GGACAGCAGC CCGCACCTTC CTGCACCCTT CAACTTGAAA
    GCAGATGATA AGCCAAAACT AGAGTTTTCC TTGCAAAGAA CTGCATCTCC TGGTCTGCGG
    CAGCCCGACA CTCCTCTGGA GAGAAGAGAT CCCAGCAGCC CAGTCCAAAC ACCCTCCTCT
    CCCCCACACA AACCTGAGCT TCCCCCCTCC GACTCAGAGA CGGCCTCAAG TGTAGCTACA
    GCCCCGACTC CTTCCATACC TGCCTCAACA GAAGAGTCAG CTGATGCCCC GAGCCCATTA
    GCTGAGCCCA GTCTCACTAA AGCTATCACA CCTGAACCAG AGAGCTCTGA GCCAGAGAAA
    TCCTCCTCAC CACCACCTCA AAGTCTGTCT GGATCCCTTA CCCAGCACGA AAAAGCCGTC
    AATGGGCTCA CGGACGTTGA CGCTGCTCCA CTGAGTGAAG AATTGGAAAC CCAACCCAGG
    GAAGCCTCTC CATTGCTGCC TACTTCCAGT GTGCCCCAGT CAGAGCCCAG GCCAGTCACA
    CCGGTGCTGG AGGAAGAGTC TGATCCAATC AATATGGACA GTCCTTTGCC TCCTGTTGAA
    GACGATGCTG GATGTCCTGA CAACGTTTCC CCCTCCCTCT CCACCTCCAC GACTGCTGCT
    ATCTCCACCA CTCCTCCTGC TCCTCCTCCA GGACTCTCGC ACCCCAGTCA GGTTTCTGCA
    GCGTTGGACA GGAGACCCTC TAATGGAGCA GAGATCAAAG AAACAGGGAA GGAAAACGAG
    GCCTTGCCGG ACAAGAGAGG AGAGCCTTTC TTACAGTCCA GAAAAAGCTC AAATCAAGCT
    ACATCTAGTG CACCAAAGAC CTGGAAGAAG CCAAAAGAGG ACATGCCTGT AGGGCAAGAT
    CAGTGTCCAG ATAAAGAGGA GGACATTGAT CAGACCAGTA TGGAACCTGC TCCTGAAAGC
    CCCAGTCCTC CTCCTGTGGA GGTGGAGAGG AGGCCATCAG TAGGAGCAGT GCTCGAGGAG
    AATGGAGAAC AGGAAGTGGA GCCTTTGAGA AACGGTGCTG AACACACTTC TGAAACTGAG
    AGCAGTGACA GCATCCACCC GCCTGCAGTC AAAGAGCCGT TGATCAAAGT ATTGCCACAG
    CCTGCAGAGA AGAATGGAAA GAAGCAGTAC AACAGAGACT TCCTGCTGGG CTTCCAGTTT
    ATGCCTGCAT GTGTGCAGAA ACCAGAAGGG CTTCCTCCCA TTTCTGATGT TGTTCTAGAT
    AAGATTAACC AAAATAAGCT GCCCATGCGG CCGGTAGACC CACGTGTGCT TTCCAGAGGC
    CCGGATTTTA CGCCAGCATT TGCGGATTTC AGCCGACCCA TTTCAGGAGG CAGAGGGGCA
    CCGTTGCTGA ATGTTGGCAT GCGACGGCCA CCTGGACGCA AGATCATCAC AAACGTGTCT
    GTGATTGATG AGGTGCAGCT GAAGACAACA GAGAATGCCT GGAAGCCTGG CATGAAGCGG
    GAAGGTGCAG TTGAAGATCC AGATGCCCAG AGAACTCAGG AGCTTTTCCG AAAAGTGCGC
    AGTATTCTCA ATAAACTGAC ACCGCAGATG TTCAGCCAGC TGATGAAGCA GGTGACTGAC
    CTCACCATCG ACACAGAGGA GCGACTCAAG GGTGTCATAG ATCTGGTCTT TGAAAAAGCA
    ATCAATGAGC CCAGCTTCTC TGTGGCCTAC GGCAACATGT GCAGCTGTCT GGCCACGTTA
    AAAGTGCCCA TGACGGATAA GCCCAACAGC ACTGTGAATT TCCGGAAGCT GCTTCTGAAC
    CGCTGTCAGA AAGAGTTTGA GAAGGACAAG ATGGATGACG ATGCCTTCGA GAAGAAACAC
    AGGGAGCTGG AGGCAGCTAC TGCGTCCAGT GAGCGGGAGA GGCTTCAGGA GGAGCTGGAA
    GAGGCCAAAG ACAAGGCCCG AAGACGCTCC ATTGGCAACA TTAAGTTCAT CGGCGAGCTC
    TTCAAGCTGC GGATGCTGAC AGAGGCCATC ATGCATGACT GCGTGGTGAA GTTGCTGAAA
    AACCATGATG AGGAATCACT GGAGTGCCTG TGTAGACTGC TCACCACTAT TGGAAAGGAC
    CTGGACTTTG AGAAAGCTAA GCCACGAATG GATCAGTACT TTAATCAGAT GGAGAAGATA
    GTTAAGGAGA GGAAGACTTC TTCACGCATT CGCTTCATGC TGCAGGATGT TATTGATCTG
    CGATTGCACA ACTGGGTTTC TCGAAGAGCA GATCAGGGCC CCAAGACCAT CGAGCAGATC
    CATAAAGACG CAAAGCTGGA GGAGCAGGAG GAACAGAGGA AAGTCCACCA GCAACTGCTT
    TCCAAAGACA ACAAGAGGAG ACCAGTGGTG CAGAGAGAGG AGACCTGGAG CACTGTGCCT
    ATGACTAAGA ACAGCAGAAC AATAGACCCC GCCAAGATTC CCAAATTCTC CAAGTCAGCG
    ATAGATGAGA AGATTCAGCT GGGGCCGAGA GCTCAGGTGA ACTGGATGAA GGGCAGCAGT
    GGTGGAGCTG GAGCAAAGGC CAGTGAGTCA GATGCGTCTC GGCCGAGTGC CAGTTTGAAT
    CGTTACTCTC CTCTGCAACC CTCGGCACTC CAGACTTCTT CTTTACCCTC CACATCTCCA
    GATTTTGATT CCAGGAGAGT TCTCGGCAGT CGTGGAAGCT CAGGACGGGA GCGCAATGAC
    AAGCCATTGA GTGCGGGTCC TGCTCGGACA GGGCCTATTT CATTGAGCAG TAGCAATAAG
    GAGACCCCCG AAGAGCTGGT TCAGGAAGTG TCCCGCAGAG ACTCCAATGC TTCAGATACA
    CCCAAGCTGC TCGTCAGTAC TGCAGACAAG AGCAGACTGG AGAACAGCCA ACCCAGAGAG
    TCTGCTGTTA AACTTGAAGC ACTCTCAGGA CCCTCTCCAG ATAAACCTGC ATTATCAGAA
    GAGGAGATGG AGAGGCGGTC CAAGTCTATA ATTGATGAGT TTTTGCACAT TAATGATTAC
    AAGGAAGCTT TGCAGTGTGT TGAGGAACTA GAGCAGTCTG CCATGCTCTA TGTGTTTGTG
    CGGGTGGGTG TGGAGTCCAC GCTGGAAAGG AGTCAGATCA CGCGAGACCA TATGGGTCAG
    CTGCTCTTCC AGCTTCTGCA GGCTGGAGTC CTCCTTAAAC TTCAGTTCTT TAAAGGGTTT
    TCTGAAACTC TTGAATTAGC AGATGATATG GCCATTGATA TTCCCCATAT ATGGCTGTAC
    CTTGCGGAGC TGGTAACCCC TGTGCTTCGA GAGGGGGGAA TCTCTATGAG AGAATTATTT
    AGTGAATTTA GCAAACCATT ACTCCCTGTG GGAAGAGCTG GGATATTATT TTCTGAAATA
    TTGCACCTTC TATGCAAACA AATGAGCCAT AAGAAAGTGG GATCATTATG GAGGGATTCT
    GGTTTGAGCT GGGCAGATTT TATACCTGAA ACAGAGGATG TGCACAGCTT CATCACAGAA
    CATAAACTAG AGTTCACTCT GTCTGATGGC TCAAACCCGA CCGAGGCTGT CTCTAAGAGA
    ACACTCTCGC CTGAGGAACT GAACAAGCAA CTTGAAAAAC TTTTACTTGA GGACATGGTT
    GGCGATGAAC AAATCTTTGA CTGGGTTGAG GCCAATCTAG ATGAATCAGA AATGAGTTCA
    GCTCCTTTCG TCAGAGCTTT AATGACTGCT GTGTGTAAAG CAGCAGTAAA AACTGAGGGC
    TCCTCGTGTA AAGTGGACTT GTCCATAATT CAGACGAGAT TGCCTGTATT ACACAAGTAC
    CTTAACTCAG ACACAGAGAG ACAGTTGCAA GCACTTTATG CACTTCAAGC ATTGATAGTA
    AAACTGGACC AGCCTGCTAA TTTGCTCCGG ATGTTTTTTG ACTGTTTGTA TGATGAGGAC
    GTGATCTCTG AAGACGCTTT CTATAAATGG GAGGTCAGTA AAGACCCCGC TGAACAGCAA
    GGCAAAGGTG TTGCCCTGAA GTCCGTCACA GCCTTCTTCA CGTGGCTGCG CGAGGCCGAG
    GAAGAGTCCG AGGACAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146819
    Clone ID Related Accession (Same CDS sequence) XM_021474544.1
    Accession Version XM_021474544.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395362.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACCAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAATA TCCACGAGTG CCGCCTCCCC TGGACGAGCG AATCTTCTCA
    ACACAGCCTG TCTCTGCTGT CTACAGTGTA CAGAGACCCC CTGGCCCCCC ATTTACTGCA
    CATGAAATCA ACAAGGGACA CCCTAACCTA GCGGCTACCC CGCCGGGTCA TGCCTCTTCC
    CCTGGACTAT CTCAGGTATC AGTTTCAACA GTATCCACTG CGCATCTCTA TGGTCATCCT
    AAAGGCTGGG AGCCAGGAGG AGGGTCTCCG TACACAACGG GACAGAATGC CGGCACGACC
    CCTCTGGTAT ACTCTCCACC AACCCAGCCA ATGAACGCAC AGCCTCAGAG CCGCCCGTTT
    GCCCCTGGGC CTCGTCCTAC CCATCACCAG GGAGGATTCA GATCCATTCA GTTTTTCCAG
    AGGACGCAGA TGCAAACGGC CCGGCCCACC ATTCCAAGCA ACACCCCACC CATTCGGCCC
    ACGTCTCAGA CCCCCACGGC AGCAGTATAT TCTCCCAATC AGCACATCAT GATGACCATG
    GCTCACATGC CCTTTCATTC CCCACAGACA GCGCAGTACT ACATCCCTCA GTATCGTCAC
    AGTGCTCCAC AGTATGTGGG ACCTCCTCAA CAGTACCCTG TCCAGCCCAC TGGACCCAGT
    ACCTTCTATG CTGCAGCCAG TCCAGGGGAG TTTCCTGCAC CTTATGCAGC AGGACCTCCC
    TACTATCCTG GGCAGCCCGT GTACACACCA TCTCCACCCA TTATAGTGCC TACACCACAA
    CAACCTCCAC CTGCTAAGAG GGAAAAGAAA ACAATCCGCA TCCGAGACCC TAACCAGGGT
    GGCAAGGATG TCACAGACGA AATATTATCA GGAGTGGGTT TGAGTCGAAA CCCAACCCCG
    CCTGTGGGAC GGCCCTCTTC CACTCCTACA CCTCCACAGT TTTTATGTCC ACACCCTCAT
    TATCCTCACA TTTTCTACCT CAAATCCCAG CAGCTGAACA GCCAGGTAGC AGATCATGGG
    CACATCATGT ATAATGTGGA CAGCAGCCCG CACCTTCCTG CACCCTTCAA CTTGAAAGCA
    GATGATAAGC CAAAACTAGA GTTTTCCTTG CAAAGAACTG CATCTCCTGG TCTGCGGCAG
    CCCGACACTC CTCTGGAGAG AAGAGATCCC AGCAGCCCAG TCCAAACACC CTCCTCTCCC
    CCACACAAAC CTGAGCTTCC CCCCTCCGAC TCAGAGACGG CCTCAAGTGT AGCTACAGCC
    CCGACTCCTT CCATACCTGC CTCAACAGAA GAGTCAGCTG ATGCCCCGAG CCCATTAGCT
    GAGCCCAGTC TCACTAAAGC TATCACACCT GAACCAGAGA GCTCTGAGCC AGAGAAATCC
    TCCTCACCAC CACCTCAAAG TCTGTCTGGA TCCCTTACCC AGCACGAAAA AGCCGTCAAT
    GGGCTCACGG ACGTTGACGC TGCTCCACTG AGTGAAGAAT TGGAAACCCA ACCCAGGGAA
    GCCTCTCCAT TGCTGCCTAC TTCCAGTGTG CCCCAGTCAG AGCCCAGGCC AGTCACACCG
    GTGCTGGAGG AAGAGTCTGA TCCAATCAAT ATGGACAGTC CTTTGCCTCC TGTTGAAGAC
    GATGCTGGAT GTCCTGACAA CGTTTCCCCC TCCCTCTCCA CCTCCACGAC TGCTGCTATC
    TCCACCACTC CTCCTGCTCC TCCTCCAGGA CTCTCGCACC CCAGTCAGGT TTCTGCAGCG
    TTGGACAGGA GACCCTCTAA TGGAGCAGAG ATCAAAGAAA CAGGGAAGGA AAACGAGGCC
    TTGCCGGACA AGAGAGGAGA GCCTTTCTTA CAGTCCAGAA AAAGCTCAAA TCAAGCTACA
    TCTAGTGCAC CAAAGACCTG GAAGAAGCCA AAAGAGGACA TGCCTGTAGG GCAAGATCAG
    TGTCCAGATA AAGAGGAGGA CATTGATCAG ACCAGTATGG AACCTGCTCC TGAAAGCCCC
    AGTCCTCCTC CTGTGGAGGT GGAGAGGAGG CCATCAGTAG GAGCAGTGCT CGAGGAGAAT
    GGAGAACAGG AAGTGGAGCC TTTGAGAAAC GGTGCTGAAC ACACTTCTGA AACTGAGAGC
    AGTGACAGCA TCCACCCGCC TGCAGTCAAA GAGCCGTTGA TCAAAGTATT GCCACAGCCT
    GCAGAGAAGA ATGGAAAGAA GCAGTACAAC AGAGACTTCC TGCTGGGCTT CCAGTTTATG
    CCTGCATGTG TGCAGAAACC AGAAGGGCTT CCTCCCATTT CTGATGTTGT TCTAGATAAG
    ATTAACCAAA ATAAGCTGCC CATGCGGCCG GTAGACCCAC GTGTGCTTTC CAGAGGCCCG
    GATTTTACGC CAGCATTTGC GGATTTCAGC CGACCCATTT CAGGAGGCAG AGGGGCACCG
    TTGCTGAATG TTGGCATGCG ACGGCCACCT GGACGCAAGA TCATCACAAA CGTGTCTGTG
    ATTGATGAGG TGCAGCTGAA GACAACAGAG AATGCCTGGA AGCCTGGCAT GAAGCGGGAA
    GGTGCAGTTG AAGATCCAGA TGCCCAGAGA ACTCAGGAGC TTTTCCGAAA AGTGCGCAGT
    ATTCTCAATA AACTGACACC GCAGATGTTC AGCCAGCTGA TGAAGCAGGT GACTGACCTC
    ACCATCGACA CAGAGGAGCG ACTCAAGGGT GTCATAGATC TGGTCTTTGA AAAAGCAATC
    AATGAGCCCA GCTTCTCTGT GGCCTACGGC AACATGTGCA GCTGTCTGGC CACGTTAAAA
    GTGCCCATGA CGGATAAGCC CAACAGCACT GTGAATTTCC GGAAGCTGCT TCTGAACCGC
    TGTCAGAAAG AGTTTGAGAA GGACAAGATG GATGACGATG CCTTCGAGAA GAAACACAGG
    GAGCTGGAGG CAGCTACTGC GTCCAGTGAG CGGGAGAGGC TTCAGGAGGA GCTGGAAGAG
    GCCAAAGACA AGGCCCGAAG ACGCTCCATT GGCAACATTA AGTTCATCGG CGAGCTCTTC
    AAGCTGCGGA TGCTGACAGA GGCCATCATG CATGACTGCG TGGTGAAGTT GCTGAAAAAC
    CATGATGAGG AATCACTGGA GTGCCTGTGT AGACTGCTCA CCACTATTGG AAAGGACCTG
    GACTTTGAGA AAGCTAAGCC ACGAATGGAT CAGTACTTTA ATCAGATGGA GAAGATAGTT
    AAGGAGAGGA AGACTTCTTC ACGCATTCGC TTCATGCTGC AGGATGTTAT TGATCTGCGA
    TTGCACAACT GGGTTTCTCG AAGAGCAGAT CAGGGCCCCA AGACCATCGA GCAGATCCAT
    AAAGACGCAA AGCTGGAGGA GCAGGAGGAA CAGAGGAAAG TCCACCAGCA ACTGCTTTCC
    AAAGACAACA AGAGGAGACC AGTGGTGCAG AGAGAGGAGA CCTGGAGCAC TGTGCCTATG
    ACTAAGAACA GCAGAACAAT AGACCCCGCC AAGATTCCCA AATTCTCCAA GTCAGCGATA
    GATGAGAAGA TTCAGCTGGG GCCGAGAGCT CAGGTGAACT GGATGAAGGG CAGCAGTGGT
    GGAGCTGGAG CAAAGGCCAG TGAGTCAGAT GCGTCTCGGC CGAGTGCCAG TTTGAATCGT
    TACTCTCCTC TGCAACCCTC GGCACTCCAG ACTTCTTCTT TACCCTCCAC ATCTCCAGAT
    TTTGATTCCA GGAGAGTTCT CGGCAGTCGT GGAAGCTCAG GACGGGAGCG CAATGACAAG
    CCATTGAGTG CGGGTCCTGC TCGGACAGGG CCTATTTCAT TGAGCAGTAG CAATAAGGAG
    ACCCCCGAAG AGCTGGTTCA GGAAGTGTCC CGCAGAGACT CCAATGCTTC AGATACACCC
    AAGCTGCTCG TCAGTACTGC AGACAAGAGC AGACTGGAGA ACAGCCAACC CAGAGAGTCT
    GCTGTTAAAC TTGAAGCACT CTCAGGACCC TCTCCAGATA AACCTGCATT ATCAGAAGAG
    GAGATGGAGA GGCGGTCCAA GTCTATAATT GATGAGTTTT TGCACATTAA TGATTACAAG
    GAAGCTTTGC AGTGTGTTGA GGAACTAGAG CAGTCTGCCA TGCTCTATGT GTTTGTGCGG
    GTGGGTGTGG AGTCCACGCT GGAAAGGAGT CAGATCACGC GAGACCATAT GGGTCAGCTG
    CTCTTCCAGC TTCTGCAGGC TGGAGTCCTC CTTAAACTTC AGTTCTTTAA AGGGTTTTCT
    GAAACTCTTG AATTAGCAGA TGATATGGCC ATTGATATTC CCCATATATG GCTGTACCTT
    GCGGAGCTGG TAACCCCTGT GCTTCGAGAG GGGGGAATCT CTATGAGAGA ATTATTTAGT
    GAATTTAGCA AACCATTACT CCCTGTGGGA AGAGCTGGGA TATTATTTTC TGAAATATTG
    CACCTTCTAT GCAAACAAAT GAGCCATAAG AAAGTGGGAT CATTATGGAG GGATTCTGGT
    TTGAGCTGGG CAGATTTTAT ACCTGAAACA GAGGATGTGC ACAGCTTCAT CACAGAACAT
    AAACTAGAGT TCACTCTGTC TGATGGCTCA AACCCGACCG AGGCTGTCTC TAAGAGAACA
    CTCTCGCCTG AGGAACTGAA CAAGCAACTT GAAAAACTTT TACTTGAGGA CATGGTTGGC
    GATGAACAAA TCTTTGACTG GGTTGAGGCC AATCTAGATG AATCAGAAAT GAGTTCAGCT
    CCTTTCGTCA GAGCTTTAAT GACTGCTGTG TGTAAAGCAG CAGTAAAAAC TGAGGGCTCC
    TCGTGTAAAG TGGACTTGTC CATAATTCAG ACGAGATTGC CTGTATTACA CAAGTACCTT
    AACTCAGACA CAGAGAGACA GTTGCAAGCA CTTTATGCAC TTCAAGCATT GATAGTAAAA
    CTGGACCAGC CTGCTAATTT GCTCCGGATG TTTTTTGACT GTTTGTATGA TGAGGACGTG
    ATCTCTGAAG ACGCTTTCTA TAAATGGGAG GTCAGTAAAG ACCCCGCTGA ACAGCAAGGC
    AAAGGTGTTG CCCTGAAGTC CGTCACAGCC TTCTTCACGT GGCTGCGCGA GGCCGAGGAA
    GAGTCCGAGG ACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330219.1
    CDS528..5705
    Translation

    Target ORF information:

    RefSeq Version XM_021474544.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474544.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACCAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAATA TCCACGAGTG CCGCCTCCCC TGGACGAGCG AATCTTCTCA
    ACACAGCCTG TCTCTGCTGT CTACAGTGTA CAGAGACCCC CTGGCCCCCC ATTTACTGCA
    CATGAAATCA ACAAGGGACA CCCTAACCTA GCGGCTACCC CGCCGGGTCA TGCCTCTTCC
    CCTGGACTAT CTCAGGTATC AGTTTCAACA GTATCCACTG CGCATCTCTA TGGTCATCCT
    AAAGGCTGGG AGCCAGGAGG AGGGTCTCCG TACACAACGG GACAGAATGC CGGCACGACC
    CCTCTGGTAT ACTCTCCACC AACCCAGCCA ATGAACGCAC AGCCTCAGAG CCGCCCGTTT
    GCCCCTGGGC CTCGTCCTAC CCATCACCAG GGAGGATTCA GATCCATTCA GTTTTTCCAG
    AGGACGCAGA TGCAAACGGC CCGGCCCACC ATTCCAAGCA ACACCCCACC CATTCGGCCC
    ACGTCTCAGA CCCCCACGGC AGCAGTATAT TCTCCCAATC AGCACATCAT GATGACCATG
    GCTCACATGC CCTTTCATTC CCCACAGACA GCGCAGTACT ACATCCCTCA GTATCGTCAC
    AGTGCTCCAC AGTATGTGGG ACCTCCTCAA CAGTACCCTG TCCAGCCCAC TGGACCCAGT
    ACCTTCTATG CTGCAGCCAG TCCAGGGGAG TTTCCTGCAC CTTATGCAGC AGGACCTCCC
    TACTATCCTG GGCAGCCCGT GTACACACCA TCTCCACCCA TTATAGTGCC TACACCACAA
    CAACCTCCAC CTGCTAAGAG GGAAAAGAAA ACAATCCGCA TCCGAGACCC TAACCAGGGT
    GGCAAGGATG TCACAGACGA AATATTATCA GGAGTGGGTT TGAGTCGAAA CCCAACCCCG
    CCTGTGGGAC GGCCCTCTTC CACTCCTACA CCTCCACAGT TTTTATGTCC ACACCCTCAT
    TATCCTCACA TTTTCTACCT CAAATCCCAG CAGCTGAACA GCCAGGTAGC AGATCATGGG
    CACATCATGT ATAATGTGGA CAGCAGCCCG CACCTTCCTG CACCCTTCAA CTTGAAAGCA
    GATGATAAGC CAAAACTAGA GTTTTCCTTG CAAAGAACTG CATCTCCTGG TCTGCGGCAG
    CCCGACACTC CTCTGGAGAG AAGAGATCCC AGCAGCCCAG TCCAAACACC CTCCTCTCCC
    CCACACAAAC CTGAGCTTCC CCCCTCCGAC TCAGAGACGG CCTCAAGTGT AGCTACAGCC
    CCGACTCCTT CCATACCTGC CTCAACAGAA GAGTCAGCTG ATGCCCCGAG CCCATTAGCT
    GAGCCCAGTC TCACTAAAGC TATCACACCT GAACCAGAGA GCTCTGAGCC AGAGAAATCC
    TCCTCACCAC CACCTCAAAG TCTGTCTGGA TCCCTTACCC AGCACGAAAA AGCCGTCAAT
    GGGCTCACGG ACGTTGACGC TGCTCCACTG AGTGAAGAAT TGGAAACCCA ACCCAGGGAA
    GCCTCTCCAT TGCTGCCTAC TTCCAGTGTG CCCCAGTCAG AGCCCAGGCC AGTCACACCG
    GTGCTGGAGG AAGAGTCTGA TCCAATCAAT ATGGACAGTC CTTTGCCTCC TGTTGAAGAC
    GATGCTGGAT GTCCTGACAA CGTTTCCCCC TCCCTCTCCA CCTCCACGAC TGCTGCTATC
    TCCACCACTC CTCCTGCTCC TCCTCCAGGA CTCTCGCACC CCAGTCAGGT TTCTGCAGCG
    TTGGACAGGA GACCCTCTAA TGGAGCAGAG ATCAAAGAAA CAGGGAAGGA AAACGAGGCC
    TTGCCGGACA AGAGAGGAGA GCCTTTCTTA CAGTCCAGAA AAAGCTCAAA TCAAGCTACA
    TCTAGTGCAC CAAAGACCTG GAAGAAGCCA AAAGAGGACA TGCCTGTAGG GCAAGATCAG
    TGTCCAGATA AAGAGGAGGA CATTGATCAG ACCAGTATGG AACCTGCTCC TGAAAGCCCC
    AGTCCTCCTC CTGTGGAGGT GGAGAGGAGG CCATCAGTAG GAGCAGTGCT CGAGGAGAAT
    GGAGAACAGG AAGTGGAGCC TTTGAGAAAC GGTGCTGAAC ACACTTCTGA AACTGAGAGC
    AGTGACAGCA TCCACCCGCC TGCAGTCAAA GAGCCGTTGA TCAAAGTATT GCCACAGCCT
    GCAGAGAAGA ATGGAAAGAA GCAGTACAAC AGAGACTTCC TGCTGGGCTT CCAGTTTATG
    CCTGCATGTG TGCAGAAACC AGAAGGGCTT CCTCCCATTT CTGATGTTGT TCTAGATAAG
    ATTAACCAAA ATAAGCTGCC CATGCGGCCG GTAGACCCAC GTGTGCTTTC CAGAGGCCCG
    GATTTTACGC CAGCATTTGC GGATTTCAGC CGACCCATTT CAGGAGGCAG AGGGGCACCG
    TTGCTGAATG TTGGCATGCG ACGGCCACCT GGACGCAAGA TCATCACAAA CGTGTCTGTG
    ATTGATGAGG TGCAGCTGAA GACAACAGAG AATGCCTGGA AGCCTGGCAT GAAGCGGGAA
    GGTGCAGTTG AAGATCCAGA TGCCCAGAGA ACTCAGGAGC TTTTCCGAAA AGTGCGCAGT
    ATTCTCAATA AACTGACACC GCAGATGTTC AGCCAGCTGA TGAAGCAGGT GACTGACCTC
    ACCATCGACA CAGAGGAGCG ACTCAAGGGT GTCATAGATC TGGTCTTTGA AAAAGCAATC
    AATGAGCCCA GCTTCTCTGT GGCCTACGGC AACATGTGCA GCTGTCTGGC CACGTTAAAA
    GTGCCCATGA CGGATAAGCC CAACAGCACT GTGAATTTCC GGAAGCTGCT TCTGAACCGC
    TGTCAGAAAG AGTTTGAGAA GGACAAGATG GATGACGATG CCTTCGAGAA GAAACACAGG
    GAGCTGGAGG CAGCTACTGC GTCCAGTGAG CGGGAGAGGC TTCAGGAGGA GCTGGAAGAG
    GCCAAAGACA AGGCCCGAAG ACGCTCCATT GGCAACATTA AGTTCATCGG CGAGCTCTTC
    AAGCTGCGGA TGCTGACAGA GGCCATCATG CATGACTGCG TGGTGAAGTT GCTGAAAAAC
    CATGATGAGG AATCACTGGA GTGCCTGTGT AGACTGCTCA CCACTATTGG AAAGGACCTG
    GACTTTGAGA AAGCTAAGCC ACGAATGGAT CAGTACTTTA ATCAGATGGA GAAGATAGTT
    AAGGAGAGGA AGACTTCTTC ACGCATTCGC TTCATGCTGC AGGATGTTAT TGATCTGCGA
    TTGCACAACT GGGTTTCTCG AAGAGCAGAT CAGGGCCCCA AGACCATCGA GCAGATCCAT
    AAAGACGCAA AGCTGGAGGA GCAGGAGGAA CAGAGGAAAG TCCACCAGCA ACTGCTTTCC
    AAAGACAACA AGAGGAGACC AGTGGTGCAG AGAGAGGAGA CCTGGAGCAC TGTGCCTATG
    ACTAAGAACA GCAGAACAAT AGACCCCGCC AAGATTCCCA AATTCTCCAA GTCAGCGATA
    GATGAGAAGA TTCAGCTGGG GCCGAGAGCT CAGGTGAACT GGATGAAGGG CAGCAGTGGT
    GGAGCTGGAG CAAAGGCCAG TGAGTCAGAT GCGTCTCGGC CGAGTGCCAG TTTGAATCGT
    TACTCTCCTC TGCAACCCTC GGCACTCCAG ACTTCTTCTT TACCCTCCAC ATCTCCAGAT
    TTTGATTCCA GGAGAGTTCT CGGCAGTCGT GGAAGCTCAG GACGGGAGCG CAATGACAAG
    CCATTGAGTG CGGGTCCTGC TCGGACAGGG CCTATTTCAT TGAGCAGTAG CAATAAGGAG
    ACCCCCGAAG AGCTGGTTCA GGAAGTGTCC CGCAGAGACT CCAATGCTTC AGATACACCC
    AAGCTGCTCG TCAGTACTGC AGACAAGAGC AGACTGGAGA ACAGCCAACC CAGAGAGTCT
    GCTGTTAAAC TTGAAGCACT CTCAGGACCC TCTCCAGATA AACCTGCATT ATCAGAAGAG
    GAGATGGAGA GGCGGTCCAA GTCTATAATT GATGAGTTTT TGCACATTAA TGATTACAAG
    GAAGCTTTGC AGTGTGTTGA GGAACTAGAG CAGTCTGCCA TGCTCTATGT GTTTGTGCGG
    GTGGGTGTGG AGTCCACGCT GGAAAGGAGT CAGATCACGC GAGACCATAT GGGTCAGCTG
    CTCTTCCAGC TTCTGCAGGC TGGAGTCCTC CTTAAACTTC AGTTCTTTAA AGGGTTTTCT
    GAAACTCTTG AATTAGCAGA TGATATGGCC ATTGATATTC CCCATATATG GCTGTACCTT
    GCGGAGCTGG TAACCCCTGT GCTTCGAGAG GGGGGAATCT CTATGAGAGA ATTATTTAGT
    GAATTTAGCA AACCATTACT CCCTGTGGGA AGAGCTGGGA TATTATTTTC TGAAATATTG
    CACCTTCTAT GCAAACAAAT GAGCCATAAG AAAGTGGGAT CATTATGGAG GGATTCTGGT
    TTGAGCTGGG CAGATTTTAT ACCTGAAACA GAGGATGTGC ACAGCTTCAT CACAGAACAT
    AAACTAGAGT TCACTCTGTC TGATGGCTCA AACCCGACCG AGGCTGTCTC TAAGAGAACA
    CTCTCGCCTG AGGAACTGAA CAAGCAACTT GAAAAACTTT TACTTGAGGA CATGGTTGGC
    GATGAACAAA TCTTTGACTG GGTTGAGGCC AATCTAGATG AATCAGAAAT GAGTTCAGCT
    CCTTTCGTCA GAGCTTTAAT GACTGCTGTG TGTAAAGCAG CAGTAAAAAC TGAGGGCTCC
    TCGTGTAAAG TGGACTTGTC CATAATTCAG ACGAGATTGC CTGTATTACA CAAGTACCTT
    AACTCAGACA CAGAGAGACA GTTGCAAGCA CTTTATGCAC TTCAAGCATT GATAGTAAAA
    CTGGACCAGC CTGCTAATTT GCTCCGGATG TTTTTTGACT GTTTGTATGA TGAGGACGTG
    ATCTCTGAAG ACGCTTTCTA TAAATGGGAG GTCAGTAAAG ACCCCGCTGA ACAGCAAGGC
    AAAGGTGTTG CCCTGAAGTC CGTCACAGCC TTCTTCACGT GGCTGCGCGA GGCCGAGGAA
    GAGTCCGAGG ACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146820
    Clone ID Related Accession (Same CDS sequence) XM_021474545.1
    Accession Version XM_021474545.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5151bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395362.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACCAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CACCAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTGCCCCT GGGCCTCGTC CTACCCATCA CCAGGGAGGA
    TTCAGATCCA TTCAGTTTTT CCAGAGGACG CAGATGCAAA CGGCCCGGCC CACCATTCCA
    AGCAACACCC CACCCATTCG GCCCACGTCT CAGACCCCCA CGGCAGCAGT ATATTCTCCC
    AATCAGCACA TCATGATGAC CATGGCTCAC ATGCCCTTTC ATTCCCCACA GACAGCGCAG
    TACTACATCC CTCAGTATCG TCACAGTGCT CCACAGTATG TGGGACCTCC TCAACAGTAC
    CCTGTCCAGC CCACTGGACC CAGTACCTTC TATGCTGCAG CCAGTCCAGG GGAGTTTCCT
    GCACCTTATG CAGCAGGACC TCCCTACTAT CCTGGGCAGC CCGTGTACAC ACCATCTCCA
    CCCATTATAG TGCCTACACC ACAACAACCT CCACCTGCTA AGAGGGAAAA GAAAACAATC
    CGCATCCGAG ACCCTAACCA GGGTGGCAAG GATGTCACAG ACGAAATATT ATCAGGAGTG
    GGTTTGAGTC GAAACCCAAC CCCGCCTGTG GGACGGCCCT CTTCCACTCC TACACCTCCA
    CAGCAGCTGA ACAGCCAGGT AGCAGATCAT GGGCACATCA TGTATAATGT GGACAGCAGC
    CCGCACCTTC CTGCACCCTT CAACTTGAAA GCAGATGATA AGCCAAAACT AGAGTTTTCC
    TTGCAAAGAA CTGCATCTCC TGGTCTGCGG CAGCCCGACA CTCCTCTGGA GAGAAGAGAT
    CCCAGCAGCC CAGTCCAAAC ACCCTCCTCT CCCCCACACA AACCTGAGCT TCCCCCCTCC
    GACTCAGAGA CGGCCTCAAG TGTAGCTACA GCCCCGACTC CTTCCATACC TGCCTCAACA
    GAAGAGTCAG CTGATGCCCC GAGCCCATTA GCTGAGCCCA GTCTCACTAA AGCTATCACA
    CCTGAACCAG AGAGCTCTGA GCCAGAGAAA TCCTCCTCAC CACCACCTCA AAGTCTGTCT
    GGATCCCTTA CCCAGCACGA AAAAGCCGTC AATGGGCTCA CGGACGTTGA CGCTGCTCCA
    CTGAGTGAAG AATTGGAAAC CCAACCCAGG GAAGCCTCTC CATTGCTGCC TACTTCCAGT
    GTGCCCCAGT CAGAGCCCAG GCCAGTCACA CCGGTGCTGG AGGAAGAGTC TGATCCAATC
    AATATGGACA GTCCTTTGCC TCCTGTTGAA GACGATGCTG GATGTCCTGA CAACGTTTCC
    CCCTCCCTCT CCACCTCCAC GACTGCTGCT ATCTCCACCA CTCCTCCTGC TCCTCCTCCA
    GGACTCTCGC ACCCCAGTCA GGTTTCTGCA GCGTTGGACA GGAGACCCTC TAATGGAGCA
    GAGATCAAAG AAACAGGGAA GGAAAACGAG GCCTTGCCGG ACAAGAGAGG AGAGCCTTTC
    TTACAGTCCA GAAAAAGCTC AAATCAAGCT ACATCTAGTG CACCAAAGAC CTGGAAGAAG
    CCAAAAGAGG ACATGCCTGT AGGGCAAGAT CAGTGTCCAG ATAAAGAGGA GGACATTGAT
    CAGACCAGTA TGGAACCTGC TCCTGAAAGC CCCAGTCCTC CTCCTGTGGA GGTGGAGAGG
    AGGCCATCAG TAGGAGCAGT GCTCGAGGAG AATGGAGAAC AGGAAGTGGA GCCTTTGAGA
    AACGGTGCTG AACACACTTC TGAAACTGAG AGCAGTGACA GCATCCACCC GCCTGCAGTC
    AAAGAGCCGT TGATCAAAGT ATTGCCACAG CCTGCAGAGA AGAATGGAAA GAAGCAGTAC
    AACAGAGACT TCCTGCTGGG CTTCCAGTTT ATGCCTGCAT GTGTGCAGAA ACCAGAAGGG
    CTTCCTCCCA TTTCTGATGT TGTTCTAGAT AAGATTAACC AAAATAAGCT GCCCATGCGG
    CCGGTAGACC CACGTGTGCT TTCCAGAGGC CCGGATTTTA CGCCAGCATT TGCGGATTTC
    AGCCGACCCA TTTCAGGAGG CAGAGGGGCA CCGTTGCTGA ATGTTGGCAT GCGACGGCCA
    CCTGGACGCA AGATCATCAC AAACGTGTCT GTGATTGATG AGGTGCAGCT GAAGACAACA
    GAGAATGCCT GGAAGCCTGG CATGAAGCGG GAAGGTGCAG TTGAAGATCC AGATGCCCAG
    AGAACTCAGG AGCTTTTCCG AAAAGTGCGC AGTATTCTCA ATAAACTGAC ACCGCAGATG
    TTCAGCCAGC TGATGAAGCA GGTGACTGAC CTCACCATCG ACACAGAGGA GCGACTCAAG
    GGTGTCATAG ATCTGGTCTT TGAAAAAGCA ATCAATGAGC CCAGCTTCTC TGTGGCCTAC
    GGCAACATGT GCAGCTGTCT GGCCACGTTA AAAGTGCCCA TGACGGATAA GCCCAACAGC
    ACTGTGAATT TCCGGAAGCT GCTTCTGAAC CGCTGTCAGA AAGAGTTTGA GAAGGACAAG
    ATGGATGACG ATGCCTTCGA GAAGAAACAC AGGGAGCTGG AGGCAGCTAC TGCGTCCAGT
    GAGCGGGAGA GGCTTCAGGA GGAGCTGGAA GAGGCCAAAG ACAAGGCCCG AAGACGCTCC
    ATTGGCAACA TTAAGTTCAT CGGCGAGCTC TTCAAGCTGC GGATGCTGAC AGAGGCCATC
    ATGCATGACT GCGTGGTGAA GTTGCTGAAA AACCATGATG AGGAATCACT GGAGTGCCTG
    TGTAGACTGC TCACCACTAT TGGAAAGGAC CTGGACTTTG AGAAAGCTAA GCCACGAATG
    GATCAGTACT TTAATCAGAT GGAGAAGATA GTTAAGGAGA GGAAGACTTC TTCACGCATT
    CGCTTCATGC TGCAGGATGT TATTGATCTG CGATTGCACA ACTGGGTTTC TCGAAGAGCA
    GATCAGGGCC CCAAGACCAT CGAGCAGATC CATAAAGACG CAAAGCTGGA GGAGCAGGAG
    GAACAGAGGA AAGTCCACCA GCAACTGCTT TCCAAAGACA ACAAGAGGAG ACCAGTGGTG
    CAGAGAGAGG AGACCTGGAG CACTGTGCCT ATGACTAAGA ACAGCAGAAC AATAGACCCC
    GCCAAGATTC CCAAATTCTC CAAGTCAGCG ATAGATGAGA AGATTCAGCT GGGGCCGAGA
    GCTCAGGTGA ACTGGATGAA GGGCAGCAGT GGTGGAGCTG GAGCAAAGGC CAGTGAGTCA
    GATGCGTCTC GGCCGAGTGC CAGTTTGAAT CGTTACTCTC CTCTGCAACC CTCGGCACTC
    CAGACTTCTT CTTTACCCTC CACATCTCCA GATTTTGATT CCAGGAGAGT TCTCGGCAGT
    CGTGGAAGCT CAGGACGGGA GCGCAATGAC AAGCCATTGA GTGCGGGTCC TGCTCGGACA
    GGGCCTATTT CATTGAGCAG TAGCAATAAG GAGACCCCCG AAGAGCTGGT TCAGGAAGTG
    TCCCGCAGAG ACTCCAATGC TTCAGATACA CCCAAGCTGC TCGTCAGTAC TGCAGACAAG
    AGCAGACTGG AGAACAGCCA ACCCAGAGAG TCTGCTGTTA AACTTGAAGC ACTCTCAGGA
    CCCTCTCCAG ATAAACCTGC ATTATCAGAA GAGGAGATGG AGAGGCGGTC CAAGTCTATA
    ATTGATGAGT TTTTGCACAT TAATGATTAC AAGGAAGCTT TGCAGTGTGT TGAGGAACTA
    GAGCAGTCTG CCATGCTCTA TGTGTTTGTG CGGGTGGGTG TGGAGTCCAC GCTGGAAAGG
    AGTCAGATCA CGCGAGACCA TATGGGTCAG CTGCTCTTCC AGCTTCTGCA GGCTGGAGTC
    CTCCTTAAAC TTCAGTTCTT TAAAGGGTTT TCTGAAACTC TTGAATTAGC AGATGATATG
    GCCATTGATA TTCCCCATAT ATGGCTGTAC CTTGCGGAGC TGGTAACCCC TGTGCTTCGA
    GAGGGGGGAA TCTCTATGAG AGAATTATTT AGTGAATTTA GCAAACCATT ACTCCCTGTG
    GGAAGAGCTG GGATATTATT TTCTGAAATA TTGCACCTTC TATGCAAACA AATGAGCCAT
    AAGAAAGTGG GATCATTATG GAGGGATTCT GGTTTGAGCT GGGCAGATTT TATACCTGAA
    ACAGAGGATG TGCACAGCTT CATCACAGAA CATAAACTAG AGTTCACTCT GTCTGATGGC
    TCAAACCCGA CCGAGGCTGT CTCTAAGAGA ACACTCTCGC CTGAGGAACT GAACAAGCAA
    CTTGAAAAAC TTTTACTTGA GGACATGGTT GGCGATGAAC AAATCTTTGA CTGGGTTGAG
    GCCAATCTAG ATGAATCAGA AATGAGTTCA GCTCCTTTCG TCAGAGCTTT AATGACTGCT
    GTGTGTAAAG CAGCAGTAAA AACTGAGGGC TCCTCGTGTA AAGTGGACTT GTCCATAATT
    CAGACGAGAT TGCCTGTATT ACACAAGTAC CTTAACTCAG ACACAGAGAG ACAGTTGCAA
    GCACTTTATG CACTTCAAGC ATTGATAGTA AAACTGGACC AGCCTGCTAA TTTGCTCCGG
    ATGTTTTTTG ACTGTTTGTA TGATGAGGAC GTGATCTCTG AAGACGCTTT CTATAAATGG
    GAGGTCAGTA AAGACCCCGC TGAACAGCAA GGCAAAGGTG TTGCCCTGAA GTCCGTCACA
    GCCTTCTTCA CGTGGCTGCG CGAGGCCGAG GAAGAGTCCG AGGACAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330220.1
    CDS528..5678
    Translation

    Target ORF information:

    RefSeq Version XM_021474545.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474545.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACCAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CACCAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTGCCCCT GGGCCTCGTC CTACCCATCA CCAGGGAGGA
    TTCAGATCCA TTCAGTTTTT CCAGAGGACG CAGATGCAAA CGGCCCGGCC CACCATTCCA
    AGCAACACCC CACCCATTCG GCCCACGTCT CAGACCCCCA CGGCAGCAGT ATATTCTCCC
    AATCAGCACA TCATGATGAC CATGGCTCAC ATGCCCTTTC ATTCCCCACA GACAGCGCAG
    TACTACATCC CTCAGTATCG TCACAGTGCT CCACAGTATG TGGGACCTCC TCAACAGTAC
    CCTGTCCAGC CCACTGGACC CAGTACCTTC TATGCTGCAG CCAGTCCAGG GGAGTTTCCT
    GCACCTTATG CAGCAGGACC TCCCTACTAT CCTGGGCAGC CCGTGTACAC ACCATCTCCA
    CCCATTATAG TGCCTACACC ACAACAACCT CCACCTGCTA AGAGGGAAAA GAAAACAATC
    CGCATCCGAG ACCCTAACCA GGGTGGCAAG GATGTCACAG ACGAAATATT ATCAGGAGTG
    GGTTTGAGTC GAAACCCAAC CCCGCCTGTG GGACGGCCCT CTTCCACTCC TACACCTCCA
    CAGCAGCTGA ACAGCCAGGT AGCAGATCAT GGGCACATCA TGTATAATGT GGACAGCAGC
    CCGCACCTTC CTGCACCCTT CAACTTGAAA GCAGATGATA AGCCAAAACT AGAGTTTTCC
    TTGCAAAGAA CTGCATCTCC TGGTCTGCGG CAGCCCGACA CTCCTCTGGA GAGAAGAGAT
    CCCAGCAGCC CAGTCCAAAC ACCCTCCTCT CCCCCACACA AACCTGAGCT TCCCCCCTCC
    GACTCAGAGA CGGCCTCAAG TGTAGCTACA GCCCCGACTC CTTCCATACC TGCCTCAACA
    GAAGAGTCAG CTGATGCCCC GAGCCCATTA GCTGAGCCCA GTCTCACTAA AGCTATCACA
    CCTGAACCAG AGAGCTCTGA GCCAGAGAAA TCCTCCTCAC CACCACCTCA AAGTCTGTCT
    GGATCCCTTA CCCAGCACGA AAAAGCCGTC AATGGGCTCA CGGACGTTGA CGCTGCTCCA
    CTGAGTGAAG AATTGGAAAC CCAACCCAGG GAAGCCTCTC CATTGCTGCC TACTTCCAGT
    GTGCCCCAGT CAGAGCCCAG GCCAGTCACA CCGGTGCTGG AGGAAGAGTC TGATCCAATC
    AATATGGACA GTCCTTTGCC TCCTGTTGAA GACGATGCTG GATGTCCTGA CAACGTTTCC
    CCCTCCCTCT CCACCTCCAC GACTGCTGCT ATCTCCACCA CTCCTCCTGC TCCTCCTCCA
    GGACTCTCGC ACCCCAGTCA GGTTTCTGCA GCGTTGGACA GGAGACCCTC TAATGGAGCA
    GAGATCAAAG AAACAGGGAA GGAAAACGAG GCCTTGCCGG ACAAGAGAGG AGAGCCTTTC
    TTACAGTCCA GAAAAAGCTC AAATCAAGCT ACATCTAGTG CACCAAAGAC CTGGAAGAAG
    CCAAAAGAGG ACATGCCTGT AGGGCAAGAT CAGTGTCCAG ATAAAGAGGA GGACATTGAT
    CAGACCAGTA TGGAACCTGC TCCTGAAAGC CCCAGTCCTC CTCCTGTGGA GGTGGAGAGG
    AGGCCATCAG TAGGAGCAGT GCTCGAGGAG AATGGAGAAC AGGAAGTGGA GCCTTTGAGA
    AACGGTGCTG AACACACTTC TGAAACTGAG AGCAGTGACA GCATCCACCC GCCTGCAGTC
    AAAGAGCCGT TGATCAAAGT ATTGCCACAG CCTGCAGAGA AGAATGGAAA GAAGCAGTAC
    AACAGAGACT TCCTGCTGGG CTTCCAGTTT ATGCCTGCAT GTGTGCAGAA ACCAGAAGGG
    CTTCCTCCCA TTTCTGATGT TGTTCTAGAT AAGATTAACC AAAATAAGCT GCCCATGCGG
    CCGGTAGACC CACGTGTGCT TTCCAGAGGC CCGGATTTTA CGCCAGCATT TGCGGATTTC
    AGCCGACCCA TTTCAGGAGG CAGAGGGGCA CCGTTGCTGA ATGTTGGCAT GCGACGGCCA
    CCTGGACGCA AGATCATCAC AAACGTGTCT GTGATTGATG AGGTGCAGCT GAAGACAACA
    GAGAATGCCT GGAAGCCTGG CATGAAGCGG GAAGGTGCAG TTGAAGATCC AGATGCCCAG
    AGAACTCAGG AGCTTTTCCG AAAAGTGCGC AGTATTCTCA ATAAACTGAC ACCGCAGATG
    TTCAGCCAGC TGATGAAGCA GGTGACTGAC CTCACCATCG ACACAGAGGA GCGACTCAAG
    GGTGTCATAG ATCTGGTCTT TGAAAAAGCA ATCAATGAGC CCAGCTTCTC TGTGGCCTAC
    GGCAACATGT GCAGCTGTCT GGCCACGTTA AAAGTGCCCA TGACGGATAA GCCCAACAGC
    ACTGTGAATT TCCGGAAGCT GCTTCTGAAC CGCTGTCAGA AAGAGTTTGA GAAGGACAAG
    ATGGATGACG ATGCCTTCGA GAAGAAACAC AGGGAGCTGG AGGCAGCTAC TGCGTCCAGT
    GAGCGGGAGA GGCTTCAGGA GGAGCTGGAA GAGGCCAAAG ACAAGGCCCG AAGACGCTCC
    ATTGGCAACA TTAAGTTCAT CGGCGAGCTC TTCAAGCTGC GGATGCTGAC AGAGGCCATC
    ATGCATGACT GCGTGGTGAA GTTGCTGAAA AACCATGATG AGGAATCACT GGAGTGCCTG
    TGTAGACTGC TCACCACTAT TGGAAAGGAC CTGGACTTTG AGAAAGCTAA GCCACGAATG
    GATCAGTACT TTAATCAGAT GGAGAAGATA GTTAAGGAGA GGAAGACTTC TTCACGCATT
    CGCTTCATGC TGCAGGATGT TATTGATCTG CGATTGCACA ACTGGGTTTC TCGAAGAGCA
    GATCAGGGCC CCAAGACCAT CGAGCAGATC CATAAAGACG CAAAGCTGGA GGAGCAGGAG
    GAACAGAGGA AAGTCCACCA GCAACTGCTT TCCAAAGACA ACAAGAGGAG ACCAGTGGTG
    CAGAGAGAGG AGACCTGGAG CACTGTGCCT ATGACTAAGA ACAGCAGAAC AATAGACCCC
    GCCAAGATTC CCAAATTCTC CAAGTCAGCG ATAGATGAGA AGATTCAGCT GGGGCCGAGA
    GCTCAGGTGA ACTGGATGAA GGGCAGCAGT GGTGGAGCTG GAGCAAAGGC CAGTGAGTCA
    GATGCGTCTC GGCCGAGTGC CAGTTTGAAT CGTTACTCTC CTCTGCAACC CTCGGCACTC
    CAGACTTCTT CTTTACCCTC CACATCTCCA GATTTTGATT CCAGGAGAGT TCTCGGCAGT
    CGTGGAAGCT CAGGACGGGA GCGCAATGAC AAGCCATTGA GTGCGGGTCC TGCTCGGACA
    GGGCCTATTT CATTGAGCAG TAGCAATAAG GAGACCCCCG AAGAGCTGGT TCAGGAAGTG
    TCCCGCAGAG ACTCCAATGC TTCAGATACA CCCAAGCTGC TCGTCAGTAC TGCAGACAAG
    AGCAGACTGG AGAACAGCCA ACCCAGAGAG TCTGCTGTTA AACTTGAAGC ACTCTCAGGA
    CCCTCTCCAG ATAAACCTGC ATTATCAGAA GAGGAGATGG AGAGGCGGTC CAAGTCTATA
    ATTGATGAGT TTTTGCACAT TAATGATTAC AAGGAAGCTT TGCAGTGTGT TGAGGAACTA
    GAGCAGTCTG CCATGCTCTA TGTGTTTGTG CGGGTGGGTG TGGAGTCCAC GCTGGAAAGG
    AGTCAGATCA CGCGAGACCA TATGGGTCAG CTGCTCTTCC AGCTTCTGCA GGCTGGAGTC
    CTCCTTAAAC TTCAGTTCTT TAAAGGGTTT TCTGAAACTC TTGAATTAGC AGATGATATG
    GCCATTGATA TTCCCCATAT ATGGCTGTAC CTTGCGGAGC TGGTAACCCC TGTGCTTCGA
    GAGGGGGGAA TCTCTATGAG AGAATTATTT AGTGAATTTA GCAAACCATT ACTCCCTGTG
    GGAAGAGCTG GGATATTATT TTCTGAAATA TTGCACCTTC TATGCAAACA AATGAGCCAT
    AAGAAAGTGG GATCATTATG GAGGGATTCT GGTTTGAGCT GGGCAGATTT TATACCTGAA
    ACAGAGGATG TGCACAGCTT CATCACAGAA CATAAACTAG AGTTCACTCT GTCTGATGGC
    TCAAACCCGA CCGAGGCTGT CTCTAAGAGA ACACTCTCGC CTGAGGAACT GAACAAGCAA
    CTTGAAAAAC TTTTACTTGA GGACATGGTT GGCGATGAAC AAATCTTTGA CTGGGTTGAG
    GCCAATCTAG ATGAATCAGA AATGAGTTCA GCTCCTTTCG TCAGAGCTTT AATGACTGCT
    GTGTGTAAAG CAGCAGTAAA AACTGAGGGC TCCTCGTGTA AAGTGGACTT GTCCATAATT
    CAGACGAGAT TGCCTGTATT ACACAAGTAC CTTAACTCAG ACACAGAGAG ACAGTTGCAA
    GCACTTTATG CACTTCAAGC ATTGATAGTA AAACTGGACC AGCCTGCTAA TTTGCTCCGG
    ATGTTTTTTG ACTGTTTGTA TGATGAGGAC GTGATCTCTG AAGACGCTTT CTATAAATGG
    GAGGTCAGTA AAGACCCCGC TGAACAGCAA GGCAAAGGTG TTGCCCTGAA GTCCGTCACA
    GCCTTCTTCA CGTGGCTGCG CGAGGCCGAG GAAGAGTCCG AGGACAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146821
    Clone ID Related Accession (Same CDS sequence) XM_021474546.1
    Accession Version XM_021474546.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395362.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACCAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CACCAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTGCCCCT GGGCCTCGTC CTACCCATCA CCAGGGAGGA
    TTCAGATCCA TTCAGTTTTT CCAGAGGACG CAGATGCAAA CGGCCCGGCC CACCATTCCA
    AGCAACACCC CACCCATTCG GCCCACGTCT CAGACCCCCA CGGCAGCAGT ATATTCTCCC
    AATCAGCACA TCATGATGAC CATGGCTCAC ATGCCCTTTC ATTCCCCACA GACAGCGCAG
    TACTACATCC CTCAGTATCG TCACAGTGCT CCACAGTATG TGGGACCTCC TCAACAGTAC
    CCTGTCCAGC CCACTGGACC CAGTACCTTC TATGCTGCAG CCAGTCCAGG GGAGTTTCCT
    GCACCTTATG CAGCAGGACC TCCCTACTAT CCTGGGCAGC CCGTGTACAC ACCATCTCCA
    CCCATTATAG TGCCTACACC ACAACAACCT CCACCTGCTA AGAGGGAAAA GAAAACAATC
    CGCATCCGAG ACCCTAACCA GGGTGGCAAG GATGTCACAG ACGAAATATT ATCAGGAGTG
    GGTTTGAGTC GAAACCCAAC CCCGCCTGTG GGACGGCCCT CTTCCACTCC TACACCTCCA
    CAGCTGAACA GCCAGGTAGC AGATCATGGG CACATCATGT ATAATGTGGA CAGCAGCCCG
    CACCTTCCTG CACCCTTCAA CTTGAAAGCA GATGATAAGC CAAAACTAGA GTTTTCCTTG
    CAAAGAACTG CATCTCCTGG TCTGCGGCAG CCCGACACTC CTCTGGAGAG AAGAGATCCC
    AGCAGCCCAG TCCAAACACC CTCCTCTCCC CCACACAAAC CTGAGCTTCC CCCCTCCGAC
    TCAGAGACGG CCTCAAGTGT AGCTACAGCC CCGACTCCTT CCATACCTGC CTCAACAGAA
    GAGTCAGCTG ATGCCCCGAG CCCATTAGCT GAGCCCAGTC TCACTAAAGC TATCACACCT
    GAACCAGAGA GCTCTGAGCC AGAGAAATCC TCCTCACCAC CACCTCAAAG TCTGTCTGGA
    TCCCTTACCC AGCACGAAAA AGCCGTCAAT GGGCTCACGG ACGTTGACGC TGCTCCACTG
    AGTGAAGAAT TGGAAACCCA ACCCAGGGAA GCCTCTCCAT TGCTGCCTAC TTCCAGTGTG
    CCCCAGTCAG AGCCCAGGCC AGTCACACCG GTGCTGGAGG AAGAGTCTGA TCCAATCAAT
    ATGGACAGTC CTTTGCCTCC TGTTGAAGAC GATGCTGGAT GTCCTGACAA CGTTTCCCCC
    TCCCTCTCCA CCTCCACGAC TGCTGCTATC TCCACCACTC CTCCTGCTCC TCCTCCAGGA
    CTCTCGCACC CCAGTCAGGT TTCTGCAGCG TTGGACAGGA GACCCTCTAA TGGAGCAGAG
    ATCAAAGAAA CAGGGAAGGA AAACGAGGCC TTGCCGGACA AGAGAGGAGA GCCTTTCTTA
    CAGTCCAGAA AAAGCTCAAA TCAAGCTACA TCTAGTGCAC CAAAGACCTG GAAGAAGCCA
    AAAGAGGACA TGCCTGTAGG GCAAGATCAG TGTCCAGATA AAGAGGAGGA CATTGATCAG
    ACCAGTATGG AACCTGCTCC TGAAAGCCCC AGTCCTCCTC CTGTGGAGGT GGAGAGGAGG
    CCATCAGTAG GAGCAGTGCT CGAGGAGAAT GGAGAACAGG AAGTGGAGCC TTTGAGAAAC
    GGTGCTGAAC ACACTTCTGA AACTGAGAGC AGTGACAGCA TCCACCCGCC TGCAGTCAAA
    GAGCCGTTGA TCAAAGTATT GCCACAGCCT GCAGAGAAGA ATGGAAAGAA GCAGTACAAC
    AGAGACTTCC TGCTGGGCTT CCAGTTTATG CCTGCATGTG TGCAGAAACC AGAAGGGCTT
    CCTCCCATTT CTGATGTTGT TCTAGATAAG ATTAACCAAA ATAAGCTGCC CATGCGGCCG
    GTAGACCCAC GTGTGCTTTC CAGAGGCCCG GATTTTACGC CAGCATTTGC GGATTTCAGC
    CGACCCATTT CAGGAGGCAG AGGGGCACCG TTGCTGAATG TTGGCATGCG ACGGCCACCT
    GGACGCAAGA TCATCACAAA CGTGTCTGTG ATTGATGAGG TGCAGCTGAA GACAACAGAG
    AATGCCTGGA AGCCTGGCAT GAAGCGGGAA GGTGCAGTTG AAGATCCAGA TGCCCAGAGA
    ACTCAGGAGC TTTTCCGAAA AGTGCGCAGT ATTCTCAATA AACTGACACC GCAGATGTTC
    AGCCAGCTGA TGAAGCAGGT GACTGACCTC ACCATCGACA CAGAGGAGCG ACTCAAGGGT
    GTCATAGATC TGGTCTTTGA AAAAGCAATC AATGAGCCCA GCTTCTCTGT GGCCTACGGC
    AACATGTGCA GCTGTCTGGC CACGTTAAAA GTGCCCATGA CGGATAAGCC CAACAGCACT
    GTGAATTTCC GGAAGCTGCT TCTGAACCGC TGTCAGAAAG AGTTTGAGAA GGACAAGATG
    GATGACGATG CCTTCGAGAA GAAACACAGG GAGCTGGAGG CAGCTACTGC GTCCAGTGAG
    CGGGAGAGGC TTCAGGAGGA GCTGGAAGAG GCCAAAGACA AGGCCCGAAG ACGCTCCATT
    GGCAACATTA AGTTCATCGG CGAGCTCTTC AAGCTGCGGA TGCTGACAGA GGCCATCATG
    CATGACTGCG TGGTGAAGTT GCTGAAAAAC CATGATGAGG AATCACTGGA GTGCCTGTGT
    AGACTGCTCA CCACTATTGG AAAGGACCTG GACTTTGAGA AAGCTAAGCC ACGAATGGAT
    CAGTACTTTA ATCAGATGGA GAAGATAGTT AAGGAGAGGA AGACTTCTTC ACGCATTCGC
    TTCATGCTGC AGGATGTTAT TGATCTGCGA TTGCACAACT GGGTTTCTCG AAGAGCAGAT
    CAGGGCCCCA AGACCATCGA GCAGATCCAT AAAGACGCAA AGCTGGAGGA GCAGGAGGAA
    CAGAGGAAAG TCCACCAGCA ACTGCTTTCC AAAGACAACA AGAGGAGACC AGTGGTGCAG
    AGAGAGGAGA CCTGGAGCAC TGTGCCTATG ACTAAGAACA GCAGAACAAT AGACCCCGCC
    AAGATTCCCA AATTCTCCAA GTCAGCGATA GATGAGAAGA TTCAGCTGGG GCCGAGAGCT
    CAGGTGAACT GGATGAAGGG CAGCAGTGGT GGAGCTGGAG CAAAGGCCAG TGAGTCAGAT
    GCGTCTCGGC CGAGTGCCAG TTTGAATCGT TACTCTCCTC TGCAACCCTC GGCACTCCAG
    ACTTCTTCTT TACCCTCCAC ATCTCCAGAT TTTGATTCCA GGAGAGTTCT CGGCAGTCGT
    GGAAGCTCAG GACGGGAGCG CAATGACAAG CCATTGAGTG CGGGTCCTGC TCGGACAGGG
    CCTATTTCAT TGAGCAGTAG CAATAAGGAG ACCCCCGAAG AGCTGGTTCA GGAAGTGTCC
    CGCAGAGACT CCAATGCTTC AGATACACCC AAGCTGCTCG TCAGTACTGC AGACAAGAGC
    AGACTGGAGA ACAGCCAACC CAGAGAGTCT GCTGTTAAAC TTGAAGCACT CTCAGGACCC
    TCTCCAGATA AACCTGCATT ATCAGAAGAG GAGATGGAGA GGCGGTCCAA GTCTATAATT
    GATGAGTTTT TGCACATTAA TGATTACAAG GAAGCTTTGC AGTGTGTTGA GGAACTAGAG
    CAGTCTGCCA TGCTCTATGT GTTTGTGCGG GTGGGTGTGG AGTCCACGCT GGAAAGGAGT
    CAGATCACGC GAGACCATAT GGGTCAGCTG CTCTTCCAGC TTCTGCAGGC TGGAGTCCTC
    CTTAAACTTC AGTTCTTTAA AGGGTTTTCT GAAACTCTTG AATTAGCAGA TGATATGGCC
    ATTGATATTC CCCATATATG GCTGTACCTT GCGGAGCTGG TAACCCCTGT GCTTCGAGAG
    GGGGGAATCT CTATGAGAGA ATTATTTAGT GAATTTAGCA AACCATTACT CCCTGTGGGA
    AGAGCTGGGA TATTATTTTC TGAAATATTG CACCTTCTAT GCAAACAAAT GAGCCATAAG
    AAAGTGGGAT CATTATGGAG GGATTCTGGT TTGAGCTGGG CAGATTTTAT ACCTGAAACA
    GAGGATGTGC ACAGCTTCAT CACAGAACAT AAACTAGAGT TCACTCTGTC TGATGGCTCA
    AACCCGACCG AGGCTGTCTC TAAGAGAACA CTCTCGCCTG AGGAACTGAA CAAGCAACTT
    GAAAAACTTT TACTTGAGGA CATGGTTGGC GATGAACAAA TCTTTGACTG GGTTGAGGCC
    AATCTAGATG AATCAGAAAT GAGTTCAGCT CCTTTCGTCA GAGCTTTAAT GACTGCTGTG
    TGTAAAGCAG CAGTAAAAAC TGAGGGCTCC TCGTGTAAAG TGGACTTGTC CATAATTCAG
    ACGAGATTGC CTGTATTACA CAAGTACCTT AACTCAGACA CAGAGAGACA GTTGCAAGCA
    CTTTATGCAC TTCAAGCATT GATAGTAAAA CTGGACCAGC CTGCTAATTT GCTCCGGATG
    TTTTTTGACT GTTTGTATGA TGAGGACGTG ATCTCTGAAG ACGCTTTCTA TAAATGGGAG
    GTCAGTAAAG ACCCCGCTGA ACAGCAAGGC AAAGGTGTTG CCCTGAAGTC CGTCACAGCC
    TTCTTCACGT GGCTGCGCGA GGCCGAGGAA GAGTCCGAGG ACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330221.1
    CDS528..5675
    Translation

    Target ORF information:

    RefSeq Version XM_021474546.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474546.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACCAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CACCAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTGCCCCT GGGCCTCGTC CTACCCATCA CCAGGGAGGA
    TTCAGATCCA TTCAGTTTTT CCAGAGGACG CAGATGCAAA CGGCCCGGCC CACCATTCCA
    AGCAACACCC CACCCATTCG GCCCACGTCT CAGACCCCCA CGGCAGCAGT ATATTCTCCC
    AATCAGCACA TCATGATGAC CATGGCTCAC ATGCCCTTTC ATTCCCCACA GACAGCGCAG
    TACTACATCC CTCAGTATCG TCACAGTGCT CCACAGTATG TGGGACCTCC TCAACAGTAC
    CCTGTCCAGC CCACTGGACC CAGTACCTTC TATGCTGCAG CCAGTCCAGG GGAGTTTCCT
    GCACCTTATG CAGCAGGACC TCCCTACTAT CCTGGGCAGC CCGTGTACAC ACCATCTCCA
    CCCATTATAG TGCCTACACC ACAACAACCT CCACCTGCTA AGAGGGAAAA GAAAACAATC
    CGCATCCGAG ACCCTAACCA GGGTGGCAAG GATGTCACAG ACGAAATATT ATCAGGAGTG
    GGTTTGAGTC GAAACCCAAC CCCGCCTGTG GGACGGCCCT CTTCCACTCC TACACCTCCA
    CAGCTGAACA GCCAGGTAGC AGATCATGGG CACATCATGT ATAATGTGGA CAGCAGCCCG
    CACCTTCCTG CACCCTTCAA CTTGAAAGCA GATGATAAGC CAAAACTAGA GTTTTCCTTG
    CAAAGAACTG CATCTCCTGG TCTGCGGCAG CCCGACACTC CTCTGGAGAG AAGAGATCCC
    AGCAGCCCAG TCCAAACACC CTCCTCTCCC CCACACAAAC CTGAGCTTCC CCCCTCCGAC
    TCAGAGACGG CCTCAAGTGT AGCTACAGCC CCGACTCCTT CCATACCTGC CTCAACAGAA
    GAGTCAGCTG ATGCCCCGAG CCCATTAGCT GAGCCCAGTC TCACTAAAGC TATCACACCT
    GAACCAGAGA GCTCTGAGCC AGAGAAATCC TCCTCACCAC CACCTCAAAG TCTGTCTGGA
    TCCCTTACCC AGCACGAAAA AGCCGTCAAT GGGCTCACGG ACGTTGACGC TGCTCCACTG
    AGTGAAGAAT TGGAAACCCA ACCCAGGGAA GCCTCTCCAT TGCTGCCTAC TTCCAGTGTG
    CCCCAGTCAG AGCCCAGGCC AGTCACACCG GTGCTGGAGG AAGAGTCTGA TCCAATCAAT
    ATGGACAGTC CTTTGCCTCC TGTTGAAGAC GATGCTGGAT GTCCTGACAA CGTTTCCCCC
    TCCCTCTCCA CCTCCACGAC TGCTGCTATC TCCACCACTC CTCCTGCTCC TCCTCCAGGA
    CTCTCGCACC CCAGTCAGGT TTCTGCAGCG TTGGACAGGA GACCCTCTAA TGGAGCAGAG
    ATCAAAGAAA CAGGGAAGGA AAACGAGGCC TTGCCGGACA AGAGAGGAGA GCCTTTCTTA
    CAGTCCAGAA AAAGCTCAAA TCAAGCTACA TCTAGTGCAC CAAAGACCTG GAAGAAGCCA
    AAAGAGGACA TGCCTGTAGG GCAAGATCAG TGTCCAGATA AAGAGGAGGA CATTGATCAG
    ACCAGTATGG AACCTGCTCC TGAAAGCCCC AGTCCTCCTC CTGTGGAGGT GGAGAGGAGG
    CCATCAGTAG GAGCAGTGCT CGAGGAGAAT GGAGAACAGG AAGTGGAGCC TTTGAGAAAC
    GGTGCTGAAC ACACTTCTGA AACTGAGAGC AGTGACAGCA TCCACCCGCC TGCAGTCAAA
    GAGCCGTTGA TCAAAGTATT GCCACAGCCT GCAGAGAAGA ATGGAAAGAA GCAGTACAAC
    AGAGACTTCC TGCTGGGCTT CCAGTTTATG CCTGCATGTG TGCAGAAACC AGAAGGGCTT
    CCTCCCATTT CTGATGTTGT TCTAGATAAG ATTAACCAAA ATAAGCTGCC CATGCGGCCG
    GTAGACCCAC GTGTGCTTTC CAGAGGCCCG GATTTTACGC CAGCATTTGC GGATTTCAGC
    CGACCCATTT CAGGAGGCAG AGGGGCACCG TTGCTGAATG TTGGCATGCG ACGGCCACCT
    GGACGCAAGA TCATCACAAA CGTGTCTGTG ATTGATGAGG TGCAGCTGAA GACAACAGAG
    AATGCCTGGA AGCCTGGCAT GAAGCGGGAA GGTGCAGTTG AAGATCCAGA TGCCCAGAGA
    ACTCAGGAGC TTTTCCGAAA AGTGCGCAGT ATTCTCAATA AACTGACACC GCAGATGTTC
    AGCCAGCTGA TGAAGCAGGT GACTGACCTC ACCATCGACA CAGAGGAGCG ACTCAAGGGT
    GTCATAGATC TGGTCTTTGA AAAAGCAATC AATGAGCCCA GCTTCTCTGT GGCCTACGGC
    AACATGTGCA GCTGTCTGGC CACGTTAAAA GTGCCCATGA CGGATAAGCC CAACAGCACT
    GTGAATTTCC GGAAGCTGCT TCTGAACCGC TGTCAGAAAG AGTTTGAGAA GGACAAGATG
    GATGACGATG CCTTCGAGAA GAAACACAGG GAGCTGGAGG CAGCTACTGC GTCCAGTGAG
    CGGGAGAGGC TTCAGGAGGA GCTGGAAGAG GCCAAAGACA AGGCCCGAAG ACGCTCCATT
    GGCAACATTA AGTTCATCGG CGAGCTCTTC AAGCTGCGGA TGCTGACAGA GGCCATCATG
    CATGACTGCG TGGTGAAGTT GCTGAAAAAC CATGATGAGG AATCACTGGA GTGCCTGTGT
    AGACTGCTCA CCACTATTGG AAAGGACCTG GACTTTGAGA AAGCTAAGCC ACGAATGGAT
    CAGTACTTTA ATCAGATGGA GAAGATAGTT AAGGAGAGGA AGACTTCTTC ACGCATTCGC
    TTCATGCTGC AGGATGTTAT TGATCTGCGA TTGCACAACT GGGTTTCTCG AAGAGCAGAT
    CAGGGCCCCA AGACCATCGA GCAGATCCAT AAAGACGCAA AGCTGGAGGA GCAGGAGGAA
    CAGAGGAAAG TCCACCAGCA ACTGCTTTCC AAAGACAACA AGAGGAGACC AGTGGTGCAG
    AGAGAGGAGA CCTGGAGCAC TGTGCCTATG ACTAAGAACA GCAGAACAAT AGACCCCGCC
    AAGATTCCCA AATTCTCCAA GTCAGCGATA GATGAGAAGA TTCAGCTGGG GCCGAGAGCT
    CAGGTGAACT GGATGAAGGG CAGCAGTGGT GGAGCTGGAG CAAAGGCCAG TGAGTCAGAT
    GCGTCTCGGC CGAGTGCCAG TTTGAATCGT TACTCTCCTC TGCAACCCTC GGCACTCCAG
    ACTTCTTCTT TACCCTCCAC ATCTCCAGAT TTTGATTCCA GGAGAGTTCT CGGCAGTCGT
    GGAAGCTCAG GACGGGAGCG CAATGACAAG CCATTGAGTG CGGGTCCTGC TCGGACAGGG
    CCTATTTCAT TGAGCAGTAG CAATAAGGAG ACCCCCGAAG AGCTGGTTCA GGAAGTGTCC
    CGCAGAGACT CCAATGCTTC AGATACACCC AAGCTGCTCG TCAGTACTGC AGACAAGAGC
    AGACTGGAGA ACAGCCAACC CAGAGAGTCT GCTGTTAAAC TTGAAGCACT CTCAGGACCC
    TCTCCAGATA AACCTGCATT ATCAGAAGAG GAGATGGAGA GGCGGTCCAA GTCTATAATT
    GATGAGTTTT TGCACATTAA TGATTACAAG GAAGCTTTGC AGTGTGTTGA GGAACTAGAG
    CAGTCTGCCA TGCTCTATGT GTTTGTGCGG GTGGGTGTGG AGTCCACGCT GGAAAGGAGT
    CAGATCACGC GAGACCATAT GGGTCAGCTG CTCTTCCAGC TTCTGCAGGC TGGAGTCCTC
    CTTAAACTTC AGTTCTTTAA AGGGTTTTCT GAAACTCTTG AATTAGCAGA TGATATGGCC
    ATTGATATTC CCCATATATG GCTGTACCTT GCGGAGCTGG TAACCCCTGT GCTTCGAGAG
    GGGGGAATCT CTATGAGAGA ATTATTTAGT GAATTTAGCA AACCATTACT CCCTGTGGGA
    AGAGCTGGGA TATTATTTTC TGAAATATTG CACCTTCTAT GCAAACAAAT GAGCCATAAG
    AAAGTGGGAT CATTATGGAG GGATTCTGGT TTGAGCTGGG CAGATTTTAT ACCTGAAACA
    GAGGATGTGC ACAGCTTCAT CACAGAACAT AAACTAGAGT TCACTCTGTC TGATGGCTCA
    AACCCGACCG AGGCTGTCTC TAAGAGAACA CTCTCGCCTG AGGAACTGAA CAAGCAACTT
    GAAAAACTTT TACTTGAGGA CATGGTTGGC GATGAACAAA TCTTTGACTG GGTTGAGGCC
    AATCTAGATG AATCAGAAAT GAGTTCAGCT CCTTTCGTCA GAGCTTTAAT GACTGCTGTG
    TGTAAAGCAG CAGTAAAAAC TGAGGGCTCC TCGTGTAAAG TGGACTTGTC CATAATTCAG
    ACGAGATTGC CTGTATTACA CAAGTACCTT AACTCAGACA CAGAGAGACA GTTGCAAGCA
    CTTTATGCAC TTCAAGCATT GATAGTAAAA CTGGACCAGC CTGCTAATTT GCTCCGGATG
    TTTTTTGACT GTTTGTATGA TGAGGACGTG ATCTCTGAAG ACGCTTTCTA TAAATGGGAG
    GTCAGTAAAG ACCCCGCTGA ACAGCAAGGC AAAGGTGTTG CCCTGAAGTC CGTCACAGCC
    TTCTTCACGT GGCTGCGCGA GGCCGAGGAA GAGTCCGAGG ACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146822
    Clone ID Related Accession (Same CDS sequence) XM_021474547.1
    Accession Version XM_021474547.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395362.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACCAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CACCAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTTTCCAG AGGACGCAGA TGCAAACGGC CCGGCCCACC
    ATTCCAAGCA ACACCCCACC CATTCGGCCC ACGTCTCAGA CCCCCACGGC AGCAGTATAT
    TCTCCCAATC AGCACATCAT GATGACCATG GCTCACATGC CCTTTCATTC CCCACAGACA
    GCGCAGTACT ACATCCCTCA GTATCGTCAC AGTGCTCCAC AGTATGTGGG ACCTCCTCAA
    CAGTACCCTG TCCAGCCCAC TGGACCCAGT ACCTTCTATG CTGCAGCCAG TCCAGGGGAG
    TTTCCTGCAC CTTATGCAGC AGGACCTCCC TACTATCCTG GGCAGCCCGT GTACACACCA
    TCTCCACCCA TTATAGTGCC TACACCACAA CAACCTCCAC CTGCTAAGAG GGAAAAGAAA
    ACAATCCGCA TCCGAGACCC TAACCAGGGT GGCAAGGATG TCACAGACGA AATATTATCA
    GGAGTGGGTT TGAGTCGAAA CCCAACCCCG CCTGTGGGAC GGCCCTCTTC CACTCCTACA
    CCTCCACAGT TTTTATGTCC ACACCCTCAT TATCCTCACA TTTTCTACCT CAAATCCCAG
    CAGCTGAACA GCCAGGTAGC AGATCATGGG CACATCATGT ATAATGTGGA CAGCAGCCCG
    CACCTTCCTG CACCCTTCAA CTTGAAAGCA GATGATAAGC CAAAACTAGA GTTTTCCTTG
    CAAAGAACTG CATCTCCTGG TCTGCGGCAG CCCGACACTC CTCTGGAGAG AAGAGATCCC
    AGCAGCCCAG TCCAAACACC CTCCTCTCCC CCACACAAAC CTGAGCTTCC CCCCTCCGAC
    TCAGAGACGG CCTCAAGTGT AGCTACAGCC CCGACTCCTT CCATACCTGC CTCAACAGAA
    GAGTCAGCTG ATGCCCCGAG CCCATTAGCT GAGCCCAGTC TCACTAAAGC TATCACACCT
    GAACCAGAGA GCTCTGAGCC AGAGAAATCC TCCTCACCAC CACCTCAAAG TCTGTCTGGA
    TCCCTTACCC AGCACGAAAA AGCCGTCAAT GGGCTCACGG ACGTTGACGC TGCTCCACTG
    AGTGAAGAAT TGGAAACCCA ACCCAGGGAA GCCTCTCCAT TGCTGCCTAC TTCCAGTGTG
    CCCCAGTCAG AGCCCAGGCC AGTCACACCG GTGCTGGAGG AAGAGTCTGA TCCAATCAAT
    ATGGACAGTC CTTTGCCTCC TGTTGAAGAC GATGCTGGAT GTCCTGACAA CGTTTCCCCC
    TCCCTCTCCA CCTCCACGAC TGCTGCTATC TCCACCACTC CTCCTGCTCC TCCTCCAGGA
    CTCTCGCACC CCAGTCAGGT TTCTGCAGCG TTGGACAGGA GACCCTCTAA TGGAGCAGAG
    ATCAAAGAAA CAGGGAAGGA AAACGAGGCC TTGCCGGACA AGAGAGGAGA GCCTTTCTTA
    CAGTCCAGAA AAAGCTCAAA TCAAGCTACA TCTAGTGCAC CAAAGACCTG GAAGAAGCCA
    AAAGAGGACA TGCCTGTAGG GCAAGATCAG TGTCCAGATA AAGAGGAGGA CATTGATCAG
    ACCAGTATGG AACCTGCTCC TGAAAGCCCC AGTCCTCCTC CTGTGGAGGT GGAGAGGAGG
    CCATCAGTAG GAGCAGTGCT CGAGGAGAAT GGAGAACAGG AAGTGGAGCC TTTGAGAAAC
    GGTGCTGAAC ACACTTCTGA AACTGAGAGC AGTGACAGCA TCCACCCGCC TGCAGTCAAA
    GAGCCGTTGA TCAAAGTATT GCCACAGCCT GCAGAGAAGA ATGGAAAGAA GCAGTACAAC
    AGAGACTTCC TGCTGGGCTT CCAGTTTATG CCTGCATGTG TGCAGAAACC AGAAGGGCTT
    CCTCCCATTT CTGATGTTGT TCTAGATAAG ATTAACCAAA ATAAGCTGCC CATGCGGCCG
    GTAGACCCAC GTGTGCTTTC CAGAGGCCCG GATTTTACGC CAGCATTTGC GGATTTCAGC
    CGACCCATTT CAGGAGGCAG AGGGGCACCG TTGCTGAATG TTGGCATGCG ACGGCCACCT
    GGACGCAAGA TCATCACAAA CGTGTCTGTG ATTGATGAGG TGCAGCTGAA GACAACAGAG
    AATGCCTGGA AGCCTGGCAT GAAGCGGGAA GGTGCAGTTG AAGATCCAGA TGCCCAGAGA
    ACTCAGGAGC TTTTCCGAAA AGTGCGCAGT ATTCTCAATA AACTGACACC GCAGATGTTC
    AGCCAGCTGA TGAAGCAGGT GACTGACCTC ACCATCGACA CAGAGGAGCG ACTCAAGGGT
    GTCATAGATC TGGTCTTTGA AAAAGCAATC AATGAGCCCA GCTTCTCTGT GGCCTACGGC
    AACATGTGCA GCTGTCTGGC CACGTTAAAA GTGCCCATGA CGGATAAGCC CAACAGCACT
    GTGAATTTCC GGAAGCTGCT TCTGAACCGC TGTCAGAAAG AGTTTGAGAA GGACAAGATG
    GATGACGATG CCTTCGAGAA GAAACACAGG GAGCTGGAGG CAGCTACTGC GTCCAGTGAG
    CGGGAGAGGC TTCAGGAGGA GCTGGAAGAG GCCAAAGACA AGGCCCGAAG ACGCTCCATT
    GGCAACATTA AGTTCATCGG CGAGCTCTTC AAGCTGCGGA TGCTGACAGA GGCCATCATG
    CATGACTGCG TGGTGAAGTT GCTGAAAAAC CATGATGAGG AATCACTGGA GTGCCTGTGT
    AGACTGCTCA CCACTATTGG AAAGGACCTG GACTTTGAGA AAGCTAAGCC ACGAATGGAT
    CAGTACTTTA ATCAGATGGA GAAGATAGTT AAGGAGAGGA AGACTTCTTC ACGCATTCGC
    TTCATGCTGC AGGATGTTAT TGATCTGCGA TTGCACAACT GGGTTTCTCG AAGAGCAGAT
    CAGGGCCCCA AGACCATCGA GCAGATCCAT AAAGACGCAA AGCTGGAGGA GCAGGAGGAA
    CAGAGGAAAG TCCACCAGCA ACTGCTTTCC AAAGACAACA AGAGGAGACC AGTGGTGCAG
    AGAGAGGAGA CCTGGAGCAC TGTGCCTATG ACTAAGAACA GCAGAACAAT AGACCCCGCC
    AAGATTCCCA AATTCTCCAA GTCAGCGATA GATGAGAAGA TTCAGCTGGG GCCGAGAGCT
    CAGGTGAACT GGATGAAGGG CAGCAGTGGT GGAGCTGGAG CAAAGGCCAG TGAGTCAGAT
    GCGTCTCGGC CGAGTGCCAG TTTGAATCGT TACTCTCCTC TGCAACCCTC GGCACTCCAG
    ACTTCTTCTT TACCCTCCAC ATCTCCAGAT TTTGATTCCA GGAGAGTTCT CGGCAGTCGT
    GGAAGCTCAG GACGGGAGCG CAATGACAAG CCATTGAGTG CGGGTCCTGC TCGGACAGGG
    CCTATTTCAT TGAGCAGTAG CAATAAGGAG ACCCCCGAAG AGCTGGTTCA GGAAGTGTCC
    CGCAGAGACT CCAATGCTTC AGATACACCC AAGCTGCTCG TCAGTACTGC AGACAAGAGC
    AGACTGGAGA ACAGCCAACC CAGAGAGTCT GCTGTTAAAC TTGAAGCACT CTCAGGACCC
    TCTCCAGATA AACCTGCATT ATCAGAAGAG GAGATGGAGA GGCGGTCCAA GTCTATAATT
    GATGAGTTTT TGCACATTAA TGATTACAAG GAAGCTTTGC AGTGTGTTGA GGAACTAGAG
    CAGTCTGCCA TGCTCTATGT GTTTGTGCGG GTGGGTGTGG AGTCCACGCT GGAAAGGAGT
    CAGATCACGC GAGACCATAT GGGTCAGCTG CTCTTCCAGC TTCTGCAGGC TGGAGTCCTC
    CTTAAACTTC AGTTCTTTAA AGGGTTTTCT GAAACTCTTG AATTAGCAGA TGATATGGCC
    ATTGATATTC CCCATATATG GCTGTACCTT GCGGAGCTGG TAACCCCTGT GCTTCGAGAG
    GGGGGAATCT CTATGAGAGA ATTATTTAGT GAATTTAGCA AACCATTACT CCCTGTGGGA
    AGAGCTGGGA TATTATTTTC TGAAATATTG CACCTTCTAT GCAAACAAAT GAGCCATAAG
    AAAGTGGGAT CATTATGGAG GGATTCTGGT TTGAGCTGGG CAGATTTTAT ACCTGAAACA
    GAGGATGTGC ACAGCTTCAT CACAGAACAT AAACTAGAGT TCACTCTGTC TGATGGCTCA
    AACCCGACCG AGGCTGTCTC TAAGAGAACA CTCTCGCCTG AGGAACTGAA CAAGCAACTT
    GAAAAACTTT TACTTGAGGA CATGGTTGGC GATGAACAAA TCTTTGACTG GGTTGAGGCC
    AATCTAGATG AATCAGAAAT GAGTTCAGCT CCTTTCGTCA GAGCTTTAAT GACTGCTGTG
    TGTAAAGCAG CAGTAAAAAC TGAGGGCTCC TCGTGTAAAG TGGACTTGTC CATAATTCAG
    ACGAGATTGC CTGTATTACA CAAGTACCTT AACTCAGACA CAGAGAGACA GTTGCAAGCA
    CTTTATGCAC TTCAAGCATT GATAGTAAAA CTGGACCAGC CTGCTAATTT GCTCCGGATG
    TTTTTTGACT GTTTGTATGA TGAGGACGTG ATCTCTGAAG ACGCTTTCTA TAAATGGGAG
    GTCAGTAAAG ACCCCGCTGA ACAGCAAGGC AAAGGTGTTG CCCTGAAGTC CGTCACAGCC
    TTCTTCACGT GGCTGCGCGA GGCCGAGGAA GAGTCCGAGG ACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330222.1
    CDS528..5675
    Translation

    Target ORF information:

    RefSeq Version XM_021474547.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474547.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACCAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CACCAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTTTCCAG AGGACGCAGA TGCAAACGGC CCGGCCCACC
    ATTCCAAGCA ACACCCCACC CATTCGGCCC ACGTCTCAGA CCCCCACGGC AGCAGTATAT
    TCTCCCAATC AGCACATCAT GATGACCATG GCTCACATGC CCTTTCATTC CCCACAGACA
    GCGCAGTACT ACATCCCTCA GTATCGTCAC AGTGCTCCAC AGTATGTGGG ACCTCCTCAA
    CAGTACCCTG TCCAGCCCAC TGGACCCAGT ACCTTCTATG CTGCAGCCAG TCCAGGGGAG
    TTTCCTGCAC CTTATGCAGC AGGACCTCCC TACTATCCTG GGCAGCCCGT GTACACACCA
    TCTCCACCCA TTATAGTGCC TACACCACAA CAACCTCCAC CTGCTAAGAG GGAAAAGAAA
    ACAATCCGCA TCCGAGACCC TAACCAGGGT GGCAAGGATG TCACAGACGA AATATTATCA
    GGAGTGGGTT TGAGTCGAAA CCCAACCCCG CCTGTGGGAC GGCCCTCTTC CACTCCTACA
    CCTCCACAGT TTTTATGTCC ACACCCTCAT TATCCTCACA TTTTCTACCT CAAATCCCAG
    CAGCTGAACA GCCAGGTAGC AGATCATGGG CACATCATGT ATAATGTGGA CAGCAGCCCG
    CACCTTCCTG CACCCTTCAA CTTGAAAGCA GATGATAAGC CAAAACTAGA GTTTTCCTTG
    CAAAGAACTG CATCTCCTGG TCTGCGGCAG CCCGACACTC CTCTGGAGAG AAGAGATCCC
    AGCAGCCCAG TCCAAACACC CTCCTCTCCC CCACACAAAC CTGAGCTTCC CCCCTCCGAC
    TCAGAGACGG CCTCAAGTGT AGCTACAGCC CCGACTCCTT CCATACCTGC CTCAACAGAA
    GAGTCAGCTG ATGCCCCGAG CCCATTAGCT GAGCCCAGTC TCACTAAAGC TATCACACCT
    GAACCAGAGA GCTCTGAGCC AGAGAAATCC TCCTCACCAC CACCTCAAAG TCTGTCTGGA
    TCCCTTACCC AGCACGAAAA AGCCGTCAAT GGGCTCACGG ACGTTGACGC TGCTCCACTG
    AGTGAAGAAT TGGAAACCCA ACCCAGGGAA GCCTCTCCAT TGCTGCCTAC TTCCAGTGTG
    CCCCAGTCAG AGCCCAGGCC AGTCACACCG GTGCTGGAGG AAGAGTCTGA TCCAATCAAT
    ATGGACAGTC CTTTGCCTCC TGTTGAAGAC GATGCTGGAT GTCCTGACAA CGTTTCCCCC
    TCCCTCTCCA CCTCCACGAC TGCTGCTATC TCCACCACTC CTCCTGCTCC TCCTCCAGGA
    CTCTCGCACC CCAGTCAGGT TTCTGCAGCG TTGGACAGGA GACCCTCTAA TGGAGCAGAG
    ATCAAAGAAA CAGGGAAGGA AAACGAGGCC TTGCCGGACA AGAGAGGAGA GCCTTTCTTA
    CAGTCCAGAA AAAGCTCAAA TCAAGCTACA TCTAGTGCAC CAAAGACCTG GAAGAAGCCA
    AAAGAGGACA TGCCTGTAGG GCAAGATCAG TGTCCAGATA AAGAGGAGGA CATTGATCAG
    ACCAGTATGG AACCTGCTCC TGAAAGCCCC AGTCCTCCTC CTGTGGAGGT GGAGAGGAGG
    CCATCAGTAG GAGCAGTGCT CGAGGAGAAT GGAGAACAGG AAGTGGAGCC TTTGAGAAAC
    GGTGCTGAAC ACACTTCTGA AACTGAGAGC AGTGACAGCA TCCACCCGCC TGCAGTCAAA
    GAGCCGTTGA TCAAAGTATT GCCACAGCCT GCAGAGAAGA ATGGAAAGAA GCAGTACAAC
    AGAGACTTCC TGCTGGGCTT CCAGTTTATG CCTGCATGTG TGCAGAAACC AGAAGGGCTT
    CCTCCCATTT CTGATGTTGT TCTAGATAAG ATTAACCAAA ATAAGCTGCC CATGCGGCCG
    GTAGACCCAC GTGTGCTTTC CAGAGGCCCG GATTTTACGC CAGCATTTGC GGATTTCAGC
    CGACCCATTT CAGGAGGCAG AGGGGCACCG TTGCTGAATG TTGGCATGCG ACGGCCACCT
    GGACGCAAGA TCATCACAAA CGTGTCTGTG ATTGATGAGG TGCAGCTGAA GACAACAGAG
    AATGCCTGGA AGCCTGGCAT GAAGCGGGAA GGTGCAGTTG AAGATCCAGA TGCCCAGAGA
    ACTCAGGAGC TTTTCCGAAA AGTGCGCAGT ATTCTCAATA AACTGACACC GCAGATGTTC
    AGCCAGCTGA TGAAGCAGGT GACTGACCTC ACCATCGACA CAGAGGAGCG ACTCAAGGGT
    GTCATAGATC TGGTCTTTGA AAAAGCAATC AATGAGCCCA GCTTCTCTGT GGCCTACGGC
    AACATGTGCA GCTGTCTGGC CACGTTAAAA GTGCCCATGA CGGATAAGCC CAACAGCACT
    GTGAATTTCC GGAAGCTGCT TCTGAACCGC TGTCAGAAAG AGTTTGAGAA GGACAAGATG
    GATGACGATG CCTTCGAGAA GAAACACAGG GAGCTGGAGG CAGCTACTGC GTCCAGTGAG
    CGGGAGAGGC TTCAGGAGGA GCTGGAAGAG GCCAAAGACA AGGCCCGAAG ACGCTCCATT
    GGCAACATTA AGTTCATCGG CGAGCTCTTC AAGCTGCGGA TGCTGACAGA GGCCATCATG
    CATGACTGCG TGGTGAAGTT GCTGAAAAAC CATGATGAGG AATCACTGGA GTGCCTGTGT
    AGACTGCTCA CCACTATTGG AAAGGACCTG GACTTTGAGA AAGCTAAGCC ACGAATGGAT
    CAGTACTTTA ATCAGATGGA GAAGATAGTT AAGGAGAGGA AGACTTCTTC ACGCATTCGC
    TTCATGCTGC AGGATGTTAT TGATCTGCGA TTGCACAACT GGGTTTCTCG AAGAGCAGAT
    CAGGGCCCCA AGACCATCGA GCAGATCCAT AAAGACGCAA AGCTGGAGGA GCAGGAGGAA
    CAGAGGAAAG TCCACCAGCA ACTGCTTTCC AAAGACAACA AGAGGAGACC AGTGGTGCAG
    AGAGAGGAGA CCTGGAGCAC TGTGCCTATG ACTAAGAACA GCAGAACAAT AGACCCCGCC
    AAGATTCCCA AATTCTCCAA GTCAGCGATA GATGAGAAGA TTCAGCTGGG GCCGAGAGCT
    CAGGTGAACT GGATGAAGGG CAGCAGTGGT GGAGCTGGAG CAAAGGCCAG TGAGTCAGAT
    GCGTCTCGGC CGAGTGCCAG TTTGAATCGT TACTCTCCTC TGCAACCCTC GGCACTCCAG
    ACTTCTTCTT TACCCTCCAC ATCTCCAGAT TTTGATTCCA GGAGAGTTCT CGGCAGTCGT
    GGAAGCTCAG GACGGGAGCG CAATGACAAG CCATTGAGTG CGGGTCCTGC TCGGACAGGG
    CCTATTTCAT TGAGCAGTAG CAATAAGGAG ACCCCCGAAG AGCTGGTTCA GGAAGTGTCC
    CGCAGAGACT CCAATGCTTC AGATACACCC AAGCTGCTCG TCAGTACTGC AGACAAGAGC
    AGACTGGAGA ACAGCCAACC CAGAGAGTCT GCTGTTAAAC TTGAAGCACT CTCAGGACCC
    TCTCCAGATA AACCTGCATT ATCAGAAGAG GAGATGGAGA GGCGGTCCAA GTCTATAATT
    GATGAGTTTT TGCACATTAA TGATTACAAG GAAGCTTTGC AGTGTGTTGA GGAACTAGAG
    CAGTCTGCCA TGCTCTATGT GTTTGTGCGG GTGGGTGTGG AGTCCACGCT GGAAAGGAGT
    CAGATCACGC GAGACCATAT GGGTCAGCTG CTCTTCCAGC TTCTGCAGGC TGGAGTCCTC
    CTTAAACTTC AGTTCTTTAA AGGGTTTTCT GAAACTCTTG AATTAGCAGA TGATATGGCC
    ATTGATATTC CCCATATATG GCTGTACCTT GCGGAGCTGG TAACCCCTGT GCTTCGAGAG
    GGGGGAATCT CTATGAGAGA ATTATTTAGT GAATTTAGCA AACCATTACT CCCTGTGGGA
    AGAGCTGGGA TATTATTTTC TGAAATATTG CACCTTCTAT GCAAACAAAT GAGCCATAAG
    AAAGTGGGAT CATTATGGAG GGATTCTGGT TTGAGCTGGG CAGATTTTAT ACCTGAAACA
    GAGGATGTGC ACAGCTTCAT CACAGAACAT AAACTAGAGT TCACTCTGTC TGATGGCTCA
    AACCCGACCG AGGCTGTCTC TAAGAGAACA CTCTCGCCTG AGGAACTGAA CAAGCAACTT
    GAAAAACTTT TACTTGAGGA CATGGTTGGC GATGAACAAA TCTTTGACTG GGTTGAGGCC
    AATCTAGATG AATCAGAAAT GAGTTCAGCT CCTTTCGTCA GAGCTTTAAT GACTGCTGTG
    TGTAAAGCAG CAGTAAAAAC TGAGGGCTCC TCGTGTAAAG TGGACTTGTC CATAATTCAG
    ACGAGATTGC CTGTATTACA CAAGTACCTT AACTCAGACA CAGAGAGACA GTTGCAAGCA
    CTTTATGCAC TTCAAGCATT GATAGTAAAA CTGGACCAGC CTGCTAATTT GCTCCGGATG
    TTTTTTGACT GTTTGTATGA TGAGGACGTG ATCTCTGAAG ACGCTTTCTA TAAATGGGAG
    GTCAGTAAAG ACCCCGCTGA ACAGCAAGGC AAAGGTGTTG CCCTGAAGTC CGTCACAGCC
    TTCTTCACGT GGCTGCGCGA GGCCGAGGAA GAGTCCGAGG ACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa151556
    Clone ID Related Accession (Same CDS sequence) XM_021469953.1
    Accession Version XM_021469953.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5202bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACTAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CAACAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTGCCCCT GGGCCTCGTC CTACCCATCA CCAGGGAGGA
    TTCAGATCCA TTCAGTTTTT CCAGAGGACG CAGATGCAAA CGGCCCGGCC CACCATTCCA
    AGCAACACCC CACCCATTCG GCCCACGTCT CAGACCCCCA CGGCAGCAGT ATATTCTCCC
    AATCAGCACA TCATGATGAC CATGGCTCAC ATGCCCTTTC ATTCCCCACA GACAGCGCAG
    TACTACATCC CTCAGTATCG TCACAGTGCT CCACAGTATG TGGGACCTCC TCAACAGTAC
    CCTGTCCAGC CGACTGGACC CAGTACCTTC TATGCTGCAG CCAGTCCAGG GGAGTTTCCT
    GCACCTTATG CAGCAGGACC TCCCTACTAT CCTGGGCAGC CCGTGTACAC ACCATCTCCA
    CCCATTATAG TGCCTACACC ACAACAACCT CCACCTGCTA AGAGGGAAAA GAAAACAATC
    CGCATCCGAG ACCCTAACCA GGGTGGCAAG GATGTCACAG ACGAAATATT ATCAGGAGTG
    GGTTTGAGTC GAAACCCAAC CCCGCCTGTG GGACGGCCCT CTTCCACTCC TACACCTCCA
    CAGTTTTTAT GTCCACACCC TCATTATCCT CACATTTTCT ACCTCAAATC CCAGCAGCTG
    AACAGCCAGG TAGCAGATCA TGGGCACATC ATGTATAATG TGGACAGCAG CCCGCACCTT
    CCAGCATCCT TCAACTTGAA AGCAGACGAT AAGCCAAAAC TAGAGTTTTC CTTGCAAAGA
    ACTGCATCTC CTGGTCTGCG GCAGCCCGAC ACTCCTCTGG AGAGAAGAGA TCCCAGCAGC
    CCAGTCCAAA CACCCTCCTC TCCCCCACAC AAACCTGAGC TTCCCCCCTC CGACTCAGAG
    ACGGCCTCAA GTGTAGCTAC AGCCCCGACT CCTTCCATAC CTGCCTCAAC AGAAGAGTCA
    GCTGATGCCC CGAGCCCATT AGCTGAGCCC AGTCTCACTA AAGCTATCAC ACCTGAACCA
    GAGAGCTCTG AGCCAGAGAA ATCCTCCTCA CCACCACCTC AAAGTCTGTC TGGATCCCTT
    ACCCAGCACG AAAAAGCCGT CAATGGGCTC ACGGATGTTG ACGCTGCTCC ACTGAGTGAA
    GAATTGGAAA CCCAACCCAG GGAAGCCTCT CCATTGCTGC CTACTTCCAG TGTGCCCCAG
    TCAGAGCCCA GGCCAGTCAC ACCGGTGCTG GAGGAAGAGT CTGATCCAAT CAATATGAAC
    AGTCCTTTGC CTCCTGTTGA GGACGATGCT GGATGTCCTG ACAACGTTTC CCCCTCCCTC
    TCCACCTCCA CGACTGCTGC TATCTCCACC ACTCCTCCTG CTCCTCCTCC AGGACTCTCG
    CACCCCAGTC AGGTTTCTGC AGCGTTGGAC AGGAGACCCT CTAATGGAGC AGAGATCAAA
    GAAACAGGGA AGGAAAACGA GGCCTTGCCG GACAAGAGAG GAGAGCCTTT CTTACAGTCC
    AGAAAAAGCT CAAATCAAGC TACATCTAGT GCACCAAAGA CCTGGAAGAA GCCAAAAGAG
    GACATGCCTG TAGGGCAAGA TCAGTGTCCA GACAAAGAGG AGGACACTGA TCAGGCCAGT
    ATGGAACCTG CTCCTGAAAG CCCCAGTCCT CCTCCTGTGG AGGTGGAGAG GAGGCCATCA
    GTAGGAGCAG TGCTCGAGGA GAATGGAGAA CAGGAAGTGG AGCCTTTGAG AAACGGTGCT
    GAACACACTT CTGAAACTGA GAGCAGTGAC AGCATCCACC CGCCTGCAGT CAAAGAGCCG
    TTGATCAAAG TATTGCCACA GCCTGCAGAG AAGAATGGAA AGAAGCAGTA CAACAGAGAC
    TTCCTGCTGG GCTTCCAGTT TATGCCTGCA TGTGTGCAGA AACCAGAAGG GCTTCCTCCC
    ATTTCTGATG TTGTTCTAGA TAAGATTAAC CAAAATAAGC TGCCCATGCG GCCGGTAGAC
    CCACGTGTGC TTTCCAGAGG CCCGGACTTT ACGCCAGCGT TTGCGGATTT CAGCCGACCC
    ATTTCAGGAG GCAGAGGGGC ACCGTTGCTG AATGTTGGCA TGCGACGGCC ACCTGGACGC
    AAGATCATCA CAAACGTGTC TGTGATTGAT GAGGTGCAGC TGAAGACAAC AGAGAATGCC
    TGGAAGCCTG GCATGAAGCG GGAAGGTGCA GTTGAAGATC CAGATGCCCA GAGAACTCAG
    GAGCTTTTCC GAAAAGTGCG CAGTATTCTC AATAAACTGA CACCGCAGAT GTTCAGCCAG
    CTGATGAAGC AGGTGACTGA CCTCACCATC GACACAGAGG AGCGACTCAA GGGTGTCATA
    GATCTGGTCT TTGAAAAAGC AATCAATGAG CCCAGCTTCT CTGTGGCCTA CGGCAACATG
    TGCAGCTGTC TGGCCACGTT AAAAGTGCCC ATGACGGATA AGCCCAACAG CACTGTGAAT
    TTCCGGAAGC TGCTTCTGAA CCGCTGTCAG AAAGAGTTTG AGAAGGACAA GATGGATGAC
    GATGCCTTTG AGAAGAAACA CAGGGAGCTG GAGGCAGCTA CTGCGTCCAG TGAGCGGGAG
    AGGCTTCAGG AGGAGCTGGA AGAGGCCAAA GACAAGGCCC GAAGACGCTC CATTGGCAAC
    ATTAAGTTCA TCGGCGAGCT CTTCAAGCTG CGGATGCTGA CAGAGGCCAT CATGCATGAC
    TGCGTGGTGA AGTTGCTGAA AAACCATGAT GAGGAATCAC TGGAGTGCCT GTGTAGACTG
    CTCACCACTA TTGGAAAGGA CCTGGACTTT GAGAAAGCTA AGCCACGAAT GGATCAGTAC
    TTTAATCAGA TGGAGAAGAT AGTTAAGGAG AGGAAGACTT CTTCACGCAT TCGCTTCATG
    CTGCAGGATG TTATTGATCT GCGATTGCAC AACTGGGTTT CTCGAAGAGC AGATCAGGGC
    CCCAAGACCA TCGAGCAGAT CCATAAAGAC GCAAAGCTGG AGGAGCAGGA GGAACAGAGG
    AAAGTCCACC AGCAACTGCT TTCCAAAGAC AACAAGAGGA GACCAGTGGT GCAGAGAGAG
    GAGACCTGGA GCACTGTGCC TATGACTAAG AACAGCAGAA CAATAGACCC CGCCAAGATT
    CCCAAATTCT CCAAGCCAGC GATAGATGAG AAGATTCAGC TGGGGCCGAG AGCTCAGGTG
    AACTGGATGA AGGGCAGCAG TGGTGGAGCT GGAGCAAAGG CCAGTGAGTC AGATGCGTCT
    CGGCCGAGTG CCAGTTTGAA TCGTTACTCT CCTCTGCAAC CCTCGGCACT CCAGACTTCT
    TCTTTACCCT CCACATCTCC AGATTTTGAT TCCAGGAGAG TTCTCGGCAG TCGTGGAAGC
    TCAGGACGGG AGCGCAATGA CAAGCCATTG AGTGCGGGTC CTGCTCGGAC AGGGCCTATT
    TCATTGAGCA GTAGCAATAA GGAGACCCCC GAAGAGCTGG TTCAGGAAGT GTCCCGCAGA
    GACTCCAATG CTTCAGATAC ACCCAAGCTG CTCGTCAGTA CTGCAGACAA GAGCAGACTG
    GAGAACAGCC AACCCAGAGA GTCTGCTGTT AAACTTGAAG CACTCTCAGG GCCCTCTCCA
    GATAAACCTG CATTATCAGA AGAGGAGATG GAGAGGCGGT CCAAGTCTAT AATTGATGAG
    TTTTTGCACA TTAATGATTA CAAGGAAGCT TTGCAGTGTG TTGAGGAACT AGAGCAGTCT
    GCCATGCTCT ATGTGTTTGT GCGGGTGGGT GTGGAGTCCA CGCTGGAAAG GAGTCAGATC
    ACGCGAGACC ATATGGGTCA GCTGCTCTTC CAGCTTCTGC AGGCTGGAGT CCTCCCTAAA
    CTTCAGTTCT TTAAAGGGTT TTCTGAAACT CTTGAATTAG CAGATGATAT GGCCATTGAT
    ATTCCCCATA TATGGCTGTA CCTTGCGGAG CTGGTAACCC CTGTGCTTCG AGAGGGGGGA
    ATCTCTATGA GAGAATTATT TAGTGAATTT AGCAAACCAT TACTCCCTGT GGGAAGAGCT
    GGGATATTAT TTTCTGAAAT ATTGCACCTT CTATGCAAAC AAATGAGCCA TAAGAAAGTG
    GGATCATTAT GGAGGGATTC TGGGTTGAGC TGGGCAGATT TTATACCTGA AACAGAGGAT
    GTGCACAGCT TCATCACAGA ACATAAACTA GAGTTCACTC TGTCTGATGG CTCAAACCCG
    ACCGAGGCTG TCTCTAAGAG AACACTCTCG CCTGAGGAAC TGAACAAGCA ACTTGAAAAA
    CTTTTACTTG AGGACATGGT TGGCGATGAA CAAATCTTCG ACTGGGTTGA GGCCAATCTA
    GATGAATCAG AAATGAGTTC AGCTCCTTTC GTCAGAGCTT TAATGACTGC TGTGTGTAAA
    GCAGCAGTAA AAACTGAGGG CTCCTCGTGT AAAGTGGACT TGTCCATAAT TCAGACGAGA
    TTGCCTGTAT TACACAAGTA CCTTAACTCA GACACAGAGA GACAGTTGCA AGCACTTTAT
    GCACTTCAAG CATTGATAGT AAAACTGGAC CAGCCTGCTA ATTTGCTCCG GATGTTTTTT
    GACTGTTTGT ATGATGAGGA CGTGATCTCT GAAGACGCTT TCTATAAATG GGAGGTCAGT
    AAAGACCCCG CTGAACAGCA AGGCAAAGGT GTTGCCCTGA AGTCCGTCAC AGCCTTCTTC
    ACGTGGCTGC GCGAGGCCGA GGAAGAGTCC GAGGACAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325628.1
    CDS458..5659
    Translation

    Target ORF information:

    RefSeq Version XM_021469953.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469953.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACTAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CAACAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTGCCCCT GGGCCTCGTC CTACCCATCA CCAGGGAGGA
    TTCAGATCCA TTCAGTTTTT CCAGAGGACG CAGATGCAAA CGGCCCGGCC CACCATTCCA
    AGCAACACCC CACCCATTCG GCCCACGTCT CAGACCCCCA CGGCAGCAGT ATATTCTCCC
    AATCAGCACA TCATGATGAC CATGGCTCAC ATGCCCTTTC ATTCCCCACA GACAGCGCAG
    TACTACATCC CTCAGTATCG TCACAGTGCT CCACAGTATG TGGGACCTCC TCAACAGTAC
    CCTGTCCAGC CGACTGGACC CAGTACCTTC TATGCTGCAG CCAGTCCAGG GGAGTTTCCT
    GCACCTTATG CAGCAGGACC TCCCTACTAT CCTGGGCAGC CCGTGTACAC ACCATCTCCA
    CCCATTATAG TGCCTACACC ACAACAACCT CCACCTGCTA AGAGGGAAAA GAAAACAATC
    CGCATCCGAG ACCCTAACCA GGGTGGCAAG GATGTCACAG ACGAAATATT ATCAGGAGTG
    GGTTTGAGTC GAAACCCAAC CCCGCCTGTG GGACGGCCCT CTTCCACTCC TACACCTCCA
    CAGTTTTTAT GTCCACACCC TCATTATCCT CACATTTTCT ACCTCAAATC CCAGCAGCTG
    AACAGCCAGG TAGCAGATCA TGGGCACATC ATGTATAATG TGGACAGCAG CCCGCACCTT
    CCAGCATCCT TCAACTTGAA AGCAGACGAT AAGCCAAAAC TAGAGTTTTC CTTGCAAAGA
    ACTGCATCTC CTGGTCTGCG GCAGCCCGAC ACTCCTCTGG AGAGAAGAGA TCCCAGCAGC
    CCAGTCCAAA CACCCTCCTC TCCCCCACAC AAACCTGAGC TTCCCCCCTC CGACTCAGAG
    ACGGCCTCAA GTGTAGCTAC AGCCCCGACT CCTTCCATAC CTGCCTCAAC AGAAGAGTCA
    GCTGATGCCC CGAGCCCATT AGCTGAGCCC AGTCTCACTA AAGCTATCAC ACCTGAACCA
    GAGAGCTCTG AGCCAGAGAA ATCCTCCTCA CCACCACCTC AAAGTCTGTC TGGATCCCTT
    ACCCAGCACG AAAAAGCCGT CAATGGGCTC ACGGATGTTG ACGCTGCTCC ACTGAGTGAA
    GAATTGGAAA CCCAACCCAG GGAAGCCTCT CCATTGCTGC CTACTTCCAG TGTGCCCCAG
    TCAGAGCCCA GGCCAGTCAC ACCGGTGCTG GAGGAAGAGT CTGATCCAAT CAATATGAAC
    AGTCCTTTGC CTCCTGTTGA GGACGATGCT GGATGTCCTG ACAACGTTTC CCCCTCCCTC
    TCCACCTCCA CGACTGCTGC TATCTCCACC ACTCCTCCTG CTCCTCCTCC AGGACTCTCG
    CACCCCAGTC AGGTTTCTGC AGCGTTGGAC AGGAGACCCT CTAATGGAGC AGAGATCAAA
    GAAACAGGGA AGGAAAACGA GGCCTTGCCG GACAAGAGAG GAGAGCCTTT CTTACAGTCC
    AGAAAAAGCT CAAATCAAGC TACATCTAGT GCACCAAAGA CCTGGAAGAA GCCAAAAGAG
    GACATGCCTG TAGGGCAAGA TCAGTGTCCA GACAAAGAGG AGGACACTGA TCAGGCCAGT
    ATGGAACCTG CTCCTGAAAG CCCCAGTCCT CCTCCTGTGG AGGTGGAGAG GAGGCCATCA
    GTAGGAGCAG TGCTCGAGGA GAATGGAGAA CAGGAAGTGG AGCCTTTGAG AAACGGTGCT
    GAACACACTT CTGAAACTGA GAGCAGTGAC AGCATCCACC CGCCTGCAGT CAAAGAGCCG
    TTGATCAAAG TATTGCCACA GCCTGCAGAG AAGAATGGAA AGAAGCAGTA CAACAGAGAC
    TTCCTGCTGG GCTTCCAGTT TATGCCTGCA TGTGTGCAGA AACCAGAAGG GCTTCCTCCC
    ATTTCTGATG TTGTTCTAGA TAAGATTAAC CAAAATAAGC TGCCCATGCG GCCGGTAGAC
    CCACGTGTGC TTTCCAGAGG CCCGGACTTT ACGCCAGCGT TTGCGGATTT CAGCCGACCC
    ATTTCAGGAG GCAGAGGGGC ACCGTTGCTG AATGTTGGCA TGCGACGGCC ACCTGGACGC
    AAGATCATCA CAAACGTGTC TGTGATTGAT GAGGTGCAGC TGAAGACAAC AGAGAATGCC
    TGGAAGCCTG GCATGAAGCG GGAAGGTGCA GTTGAAGATC CAGATGCCCA GAGAACTCAG
    GAGCTTTTCC GAAAAGTGCG CAGTATTCTC AATAAACTGA CACCGCAGAT GTTCAGCCAG
    CTGATGAAGC AGGTGACTGA CCTCACCATC GACACAGAGG AGCGACTCAA GGGTGTCATA
    GATCTGGTCT TTGAAAAAGC AATCAATGAG CCCAGCTTCT CTGTGGCCTA CGGCAACATG
    TGCAGCTGTC TGGCCACGTT AAAAGTGCCC ATGACGGATA AGCCCAACAG CACTGTGAAT
    TTCCGGAAGC TGCTTCTGAA CCGCTGTCAG AAAGAGTTTG AGAAGGACAA GATGGATGAC
    GATGCCTTTG AGAAGAAACA CAGGGAGCTG GAGGCAGCTA CTGCGTCCAG TGAGCGGGAG
    AGGCTTCAGG AGGAGCTGGA AGAGGCCAAA GACAAGGCCC GAAGACGCTC CATTGGCAAC
    ATTAAGTTCA TCGGCGAGCT CTTCAAGCTG CGGATGCTGA CAGAGGCCAT CATGCATGAC
    TGCGTGGTGA AGTTGCTGAA AAACCATGAT GAGGAATCAC TGGAGTGCCT GTGTAGACTG
    CTCACCACTA TTGGAAAGGA CCTGGACTTT GAGAAAGCTA AGCCACGAAT GGATCAGTAC
    TTTAATCAGA TGGAGAAGAT AGTTAAGGAG AGGAAGACTT CTTCACGCAT TCGCTTCATG
    CTGCAGGATG TTATTGATCT GCGATTGCAC AACTGGGTTT CTCGAAGAGC AGATCAGGGC
    CCCAAGACCA TCGAGCAGAT CCATAAAGAC GCAAAGCTGG AGGAGCAGGA GGAACAGAGG
    AAAGTCCACC AGCAACTGCT TTCCAAAGAC AACAAGAGGA GACCAGTGGT GCAGAGAGAG
    GAGACCTGGA GCACTGTGCC TATGACTAAG AACAGCAGAA CAATAGACCC CGCCAAGATT
    CCCAAATTCT CCAAGCCAGC GATAGATGAG AAGATTCAGC TGGGGCCGAG AGCTCAGGTG
    AACTGGATGA AGGGCAGCAG TGGTGGAGCT GGAGCAAAGG CCAGTGAGTC AGATGCGTCT
    CGGCCGAGTG CCAGTTTGAA TCGTTACTCT CCTCTGCAAC CCTCGGCACT CCAGACTTCT
    TCTTTACCCT CCACATCTCC AGATTTTGAT TCCAGGAGAG TTCTCGGCAG TCGTGGAAGC
    TCAGGACGGG AGCGCAATGA CAAGCCATTG AGTGCGGGTC CTGCTCGGAC AGGGCCTATT
    TCATTGAGCA GTAGCAATAA GGAGACCCCC GAAGAGCTGG TTCAGGAAGT GTCCCGCAGA
    GACTCCAATG CTTCAGATAC ACCCAAGCTG CTCGTCAGTA CTGCAGACAA GAGCAGACTG
    GAGAACAGCC AACCCAGAGA GTCTGCTGTT AAACTTGAAG CACTCTCAGG GCCCTCTCCA
    GATAAACCTG CATTATCAGA AGAGGAGATG GAGAGGCGGT CCAAGTCTAT AATTGATGAG
    TTTTTGCACA TTAATGATTA CAAGGAAGCT TTGCAGTGTG TTGAGGAACT AGAGCAGTCT
    GCCATGCTCT ATGTGTTTGT GCGGGTGGGT GTGGAGTCCA CGCTGGAAAG GAGTCAGATC
    ACGCGAGACC ATATGGGTCA GCTGCTCTTC CAGCTTCTGC AGGCTGGAGT CCTCCCTAAA
    CTTCAGTTCT TTAAAGGGTT TTCTGAAACT CTTGAATTAG CAGATGATAT GGCCATTGAT
    ATTCCCCATA TATGGCTGTA CCTTGCGGAG CTGGTAACCC CTGTGCTTCG AGAGGGGGGA
    ATCTCTATGA GAGAATTATT TAGTGAATTT AGCAAACCAT TACTCCCTGT GGGAAGAGCT
    GGGATATTAT TTTCTGAAAT ATTGCACCTT CTATGCAAAC AAATGAGCCA TAAGAAAGTG
    GGATCATTAT GGAGGGATTC TGGGTTGAGC TGGGCAGATT TTATACCTGA AACAGAGGAT
    GTGCACAGCT TCATCACAGA ACATAAACTA GAGTTCACTC TGTCTGATGG CTCAAACCCG
    ACCGAGGCTG TCTCTAAGAG AACACTCTCG CCTGAGGAAC TGAACAAGCA ACTTGAAAAA
    CTTTTACTTG AGGACATGGT TGGCGATGAA CAAATCTTCG ACTGGGTTGA GGCCAATCTA
    GATGAATCAG AAATGAGTTC AGCTCCTTTC GTCAGAGCTT TAATGACTGC TGTGTGTAAA
    GCAGCAGTAA AAACTGAGGG CTCCTCGTGT AAAGTGGACT TGTCCATAAT TCAGACGAGA
    TTGCCTGTAT TACACAAGTA CCTTAACTCA GACACAGAGA GACAGTTGCA AGCACTTTAT
    GCACTTCAAG CATTGATAGT AAAACTGGAC CAGCCTGCTA ATTTGCTCCG GATGTTTTTT
    GACTGTTTGT ATGATGAGGA CGTGATCTCT GAAGACGCTT TCTATAAATG GGAGGTCAGT
    AAAGACCCCG CTGAACAGCA AGGCAAAGGT GTTGCCCTGA AGTCCGTCAC AGCCTTCTTC
    ACGTGGCTGC GCGAGGCCGA GGAAGAGTCC GAGGACAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa151557
    Clone ID Related Accession (Same CDS sequence) XM_021469954.1
    Accession Version XM_021469954.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5199bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACTAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CAACAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTGCCCCT GGGCCTCGTC CTACCCATCA CCAGGGAGGA
    TTCAGATCCA TTCAGTTTTT CCAGAGGACG CAGATGCAAA CGGCCCGGCC CACCATTCCA
    AGCAACACCC CACCCATTCG GCCCACGTCT CAGACCCCCA CGGCAGCAGT ATATTCTCCC
    AATCAGCACA TCATGATGAC CATGGCTCAC ATGCCCTTTC ATTCCCCACA GACAGCGCAG
    TACTACATCC CTCAGTATCG TCACAGTGCT CCACAGTATG TGGGACCTCC TCAACAGTAC
    CCTGTCCAGC CGACTGGACC CAGTACCTTC TATGCTGCAG CCAGTCCAGG GGAGTTTCCT
    GCACCTTATG CAGGACCTCC CTACTATCCT GGGCAGCCCG TGTACACACC ATCTCCACCC
    ATTATAGTGC CTACACCACA ACAACCTCCA CCTGCTAAGA GGGAAAAGAA AACAATCCGC
    ATCCGAGACC CTAACCAGGG TGGCAAGGAT GTCACAGACG AAATATTATC AGGAGTGGGT
    TTGAGTCGAA ACCCAACCCC GCCTGTGGGA CGGCCCTCTT CCACTCCTAC ACCTCCACAG
    TTTTTATGTC CACACCCTCA TTATCCTCAC ATTTTCTACC TCAAATCCCA GCAGCTGAAC
    AGCCAGGTAG CAGATCATGG GCACATCATG TATAATGTGG ACAGCAGCCC GCACCTTCCA
    GCATCCTTCA ACTTGAAAGC AGACGATAAG CCAAAACTAG AGTTTTCCTT GCAAAGAACT
    GCATCTCCTG GTCTGCGGCA GCCCGACACT CCTCTGGAGA GAAGAGATCC CAGCAGCCCA
    GTCCAAACAC CCTCCTCTCC CCCACACAAA CCTGAGCTTC CCCCCTCCGA CTCAGAGACG
    GCCTCAAGTG TAGCTACAGC CCCGACTCCT TCCATACCTG CCTCAACAGA AGAGTCAGCT
    GATGCCCCGA GCCCATTAGC TGAGCCCAGT CTCACTAAAG CTATCACACC TGAACCAGAG
    AGCTCTGAGC CAGAGAAATC CTCCTCACCA CCACCTCAAA GTCTGTCTGG ATCCCTTACC
    CAGCACGAAA AAGCCGTCAA TGGGCTCACG GATGTTGACG CTGCTCCACT GAGTGAAGAA
    TTGGAAACCC AACCCAGGGA AGCCTCTCCA TTGCTGCCTA CTTCCAGTGT GCCCCAGTCA
    GAGCCCAGGC CAGTCACACC GGTGCTGGAG GAAGAGTCTG ATCCAATCAA TATGAACAGT
    CCTTTGCCTC CTGTTGAGGA CGATGCTGGA TGTCCTGACA ACGTTTCCCC CTCCCTCTCC
    ACCTCCACGA CTGCTGCTAT CTCCACCACT CCTCCTGCTC CTCCTCCAGG ACTCTCGCAC
    CCCAGTCAGG TTTCTGCAGC GTTGGACAGG AGACCCTCTA ATGGAGCAGA GATCAAAGAA
    ACAGGGAAGG AAAACGAGGC CTTGCCGGAC AAGAGAGGAG AGCCTTTCTT ACAGTCCAGA
    AAAAGCTCAA ATCAAGCTAC ATCTAGTGCA CCAAAGACCT GGAAGAAGCC AAAAGAGGAC
    ATGCCTGTAG GGCAAGATCA GTGTCCAGAC AAAGAGGAGG ACACTGATCA GGCCAGTATG
    GAACCTGCTC CTGAAAGCCC CAGTCCTCCT CCTGTGGAGG TGGAGAGGAG GCCATCAGTA
    GGAGCAGTGC TCGAGGAGAA TGGAGAACAG GAAGTGGAGC CTTTGAGAAA CGGTGCTGAA
    CACACTTCTG AAACTGAGAG CAGTGACAGC ATCCACCCGC CTGCAGTCAA AGAGCCGTTG
    ATCAAAGTAT TGCCACAGCC TGCAGAGAAG AATGGAAAGA AGCAGTACAA CAGAGACTTC
    CTGCTGGGCT TCCAGTTTAT GCCTGCATGT GTGCAGAAAC CAGAAGGGCT TCCTCCCATT
    TCTGATGTTG TTCTAGATAA GATTAACCAA AATAAGCTGC CCATGCGGCC GGTAGACCCA
    CGTGTGCTTT CCAGAGGCCC GGACTTTACG CCAGCGTTTG CGGATTTCAG CCGACCCATT
    TCAGGAGGCA GAGGGGCACC GTTGCTGAAT GTTGGCATGC GACGGCCACC TGGACGCAAG
    ATCATCACAA ACGTGTCTGT GATTGATGAG GTGCAGCTGA AGACAACAGA GAATGCCTGG
    AAGCCTGGCA TGAAGCGGGA AGGTGCAGTT GAAGATCCAG ATGCCCAGAG AACTCAGGAG
    CTTTTCCGAA AAGTGCGCAG TATTCTCAAT AAACTGACAC CGCAGATGTT CAGCCAGCTG
    ATGAAGCAGG TGACTGACCT CACCATCGAC ACAGAGGAGC GACTCAAGGG TGTCATAGAT
    CTGGTCTTTG AAAAAGCAAT CAATGAGCCC AGCTTCTCTG TGGCCTACGG CAACATGTGC
    AGCTGTCTGG CCACGTTAAA AGTGCCCATG ACGGATAAGC CCAACAGCAC TGTGAATTTC
    CGGAAGCTGC TTCTGAACCG CTGTCAGAAA GAGTTTGAGA AGGACAAGAT GGATGACGAT
    GCCTTTGAGA AGAAACACAG GGAGCTGGAG GCAGCTACTG CGTCCAGTGA GCGGGAGAGG
    CTTCAGGAGG AGCTGGAAGA GGCCAAAGAC AAGGCCCGAA GACGCTCCAT TGGCAACATT
    AAGTTCATCG GCGAGCTCTT CAAGCTGCGG ATGCTGACAG AGGCCATCAT GCATGACTGC
    GTGGTGAAGT TGCTGAAAAA CCATGATGAG GAATCACTGG AGTGCCTGTG TAGACTGCTC
    ACCACTATTG GAAAGGACCT GGACTTTGAG AAAGCTAAGC CACGAATGGA TCAGTACTTT
    AATCAGATGG AGAAGATAGT TAAGGAGAGG AAGACTTCTT CACGCATTCG CTTCATGCTG
    CAGGATGTTA TTGATCTGCG ATTGCACAAC TGGGTTTCTC GAAGAGCAGA TCAGGGCCCC
    AAGACCATCG AGCAGATCCA TAAAGACGCA AAGCTGGAGG AGCAGGAGGA ACAGAGGAAA
    GTCCACCAGC AACTGCTTTC CAAAGACAAC AAGAGGAGAC CAGTGGTGCA GAGAGAGGAG
    ACCTGGAGCA CTGTGCCTAT GACTAAGAAC AGCAGAACAA TAGACCCCGC CAAGATTCCC
    AAATTCTCCA AGCCAGCGAT AGATGAGAAG ATTCAGCTGG GGCCGAGAGC TCAGGTGAAC
    TGGATGAAGG GCAGCAGTGG TGGAGCTGGA GCAAAGGCCA GTGAGTCAGA TGCGTCTCGG
    CCGAGTGCCA GTTTGAATCG TTACTCTCCT CTGCAACCCT CGGCACTCCA GACTTCTTCT
    TTACCCTCCA CATCTCCAGA TTTTGATTCC AGGAGAGTTC TCGGCAGTCG TGGAAGCTCA
    GGACGGGAGC GCAATGACAA GCCATTGAGT GCGGGTCCTG CTCGGACAGG GCCTATTTCA
    TTGAGCAGTA GCAATAAGGA GACCCCCGAA GAGCTGGTTC AGGAAGTGTC CCGCAGAGAC
    TCCAATGCTT CAGATACACC CAAGCTGCTC GTCAGTACTG CAGACAAGAG CAGACTGGAG
    AACAGCCAAC CCAGAGAGTC TGCTGTTAAA CTTGAAGCAC TCTCAGGGCC CTCTCCAGAT
    AAACCTGCAT TATCAGAAGA GGAGATGGAG AGGCGGTCCA AGTCTATAAT TGATGAGTTT
    TTGCACATTA ATGATTACAA GGAAGCTTTG CAGTGTGTTG AGGAACTAGA GCAGTCTGCC
    ATGCTCTATG TGTTTGTGCG GGTGGGTGTG GAGTCCACGC TGGAAAGGAG TCAGATCACG
    CGAGACCATA TGGGTCAGCT GCTCTTCCAG CTTCTGCAGG CTGGAGTCCT CCCTAAACTT
    CAGTTCTTTA AAGGGTTTTC TGAAACTCTT GAATTAGCAG ATGATATGGC CATTGATATT
    CCCCATATAT GGCTGTACCT TGCGGAGCTG GTAACCCCTG TGCTTCGAGA GGGGGGAATC
    TCTATGAGAG AATTATTTAG TGAATTTAGC AAACCATTAC TCCCTGTGGG AAGAGCTGGG
    ATATTATTTT CTGAAATATT GCACCTTCTA TGCAAACAAA TGAGCCATAA GAAAGTGGGA
    TCATTATGGA GGGATTCTGG GTTGAGCTGG GCAGATTTTA TACCTGAAAC AGAGGATGTG
    CACAGCTTCA TCACAGAACA TAAACTAGAG TTCACTCTGT CTGATGGCTC AAACCCGACC
    GAGGCTGTCT CTAAGAGAAC ACTCTCGCCT GAGGAACTGA ACAAGCAACT TGAAAAACTT
    TTACTTGAGG ACATGGTTGG CGATGAACAA ATCTTCGACT GGGTTGAGGC CAATCTAGAT
    GAATCAGAAA TGAGTTCAGC TCCTTTCGTC AGAGCTTTAA TGACTGCTGT GTGTAAAGCA
    GCAGTAAAAA CTGAGGGCTC CTCGTGTAAA GTGGACTTGT CCATAATTCA GACGAGATTG
    CCTGTATTAC ACAAGTACCT TAACTCAGAC ACAGAGAGAC AGTTGCAAGC ACTTTATGCA
    CTTCAAGCAT TGATAGTAAA ACTGGACCAG CCTGCTAATT TGCTCCGGAT GTTTTTTGAC
    TGTTTGTATG ATGAGGACGT GATCTCTGAA GACGCTTTCT ATAAATGGGA GGTCAGTAAA
    GACCCCGCTG AACAGCAAGG CAAAGGTGTT GCCCTGAAGT CCGTCACAGC CTTCTTCACG
    TGGCTGCGCG AGGCCGAGGA AGAGTCCGAG GACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325629.1
    CDS458..5656
    Translation

    Target ORF information:

    RefSeq Version XM_021469954.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469954.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACTAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CAACAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTGCCCCT GGGCCTCGTC CTACCCATCA CCAGGGAGGA
    TTCAGATCCA TTCAGTTTTT CCAGAGGACG CAGATGCAAA CGGCCCGGCC CACCATTCCA
    AGCAACACCC CACCCATTCG GCCCACGTCT CAGACCCCCA CGGCAGCAGT ATATTCTCCC
    AATCAGCACA TCATGATGAC CATGGCTCAC ATGCCCTTTC ATTCCCCACA GACAGCGCAG
    TACTACATCC CTCAGTATCG TCACAGTGCT CCACAGTATG TGGGACCTCC TCAACAGTAC
    CCTGTCCAGC CGACTGGACC CAGTACCTTC TATGCTGCAG CCAGTCCAGG GGAGTTTCCT
    GCACCTTATG CAGGACCTCC CTACTATCCT GGGCAGCCCG TGTACACACC ATCTCCACCC
    ATTATAGTGC CTACACCACA ACAACCTCCA CCTGCTAAGA GGGAAAAGAA AACAATCCGC
    ATCCGAGACC CTAACCAGGG TGGCAAGGAT GTCACAGACG AAATATTATC AGGAGTGGGT
    TTGAGTCGAA ACCCAACCCC GCCTGTGGGA CGGCCCTCTT CCACTCCTAC ACCTCCACAG
    TTTTTATGTC CACACCCTCA TTATCCTCAC ATTTTCTACC TCAAATCCCA GCAGCTGAAC
    AGCCAGGTAG CAGATCATGG GCACATCATG TATAATGTGG ACAGCAGCCC GCACCTTCCA
    GCATCCTTCA ACTTGAAAGC AGACGATAAG CCAAAACTAG AGTTTTCCTT GCAAAGAACT
    GCATCTCCTG GTCTGCGGCA GCCCGACACT CCTCTGGAGA GAAGAGATCC CAGCAGCCCA
    GTCCAAACAC CCTCCTCTCC CCCACACAAA CCTGAGCTTC CCCCCTCCGA CTCAGAGACG
    GCCTCAAGTG TAGCTACAGC CCCGACTCCT TCCATACCTG CCTCAACAGA AGAGTCAGCT
    GATGCCCCGA GCCCATTAGC TGAGCCCAGT CTCACTAAAG CTATCACACC TGAACCAGAG
    AGCTCTGAGC CAGAGAAATC CTCCTCACCA CCACCTCAAA GTCTGTCTGG ATCCCTTACC
    CAGCACGAAA AAGCCGTCAA TGGGCTCACG GATGTTGACG CTGCTCCACT GAGTGAAGAA
    TTGGAAACCC AACCCAGGGA AGCCTCTCCA TTGCTGCCTA CTTCCAGTGT GCCCCAGTCA
    GAGCCCAGGC CAGTCACACC GGTGCTGGAG GAAGAGTCTG ATCCAATCAA TATGAACAGT
    CCTTTGCCTC CTGTTGAGGA CGATGCTGGA TGTCCTGACA ACGTTTCCCC CTCCCTCTCC
    ACCTCCACGA CTGCTGCTAT CTCCACCACT CCTCCTGCTC CTCCTCCAGG ACTCTCGCAC
    CCCAGTCAGG TTTCTGCAGC GTTGGACAGG AGACCCTCTA ATGGAGCAGA GATCAAAGAA
    ACAGGGAAGG AAAACGAGGC CTTGCCGGAC AAGAGAGGAG AGCCTTTCTT ACAGTCCAGA
    AAAAGCTCAA ATCAAGCTAC ATCTAGTGCA CCAAAGACCT GGAAGAAGCC AAAAGAGGAC
    ATGCCTGTAG GGCAAGATCA GTGTCCAGAC AAAGAGGAGG ACACTGATCA GGCCAGTATG
    GAACCTGCTC CTGAAAGCCC CAGTCCTCCT CCTGTGGAGG TGGAGAGGAG GCCATCAGTA
    GGAGCAGTGC TCGAGGAGAA TGGAGAACAG GAAGTGGAGC CTTTGAGAAA CGGTGCTGAA
    CACACTTCTG AAACTGAGAG CAGTGACAGC ATCCACCCGC CTGCAGTCAA AGAGCCGTTG
    ATCAAAGTAT TGCCACAGCC TGCAGAGAAG AATGGAAAGA AGCAGTACAA CAGAGACTTC
    CTGCTGGGCT TCCAGTTTAT GCCTGCATGT GTGCAGAAAC CAGAAGGGCT TCCTCCCATT
    TCTGATGTTG TTCTAGATAA GATTAACCAA AATAAGCTGC CCATGCGGCC GGTAGACCCA
    CGTGTGCTTT CCAGAGGCCC GGACTTTACG CCAGCGTTTG CGGATTTCAG CCGACCCATT
    TCAGGAGGCA GAGGGGCACC GTTGCTGAAT GTTGGCATGC GACGGCCACC TGGACGCAAG
    ATCATCACAA ACGTGTCTGT GATTGATGAG GTGCAGCTGA AGACAACAGA GAATGCCTGG
    AAGCCTGGCA TGAAGCGGGA AGGTGCAGTT GAAGATCCAG ATGCCCAGAG AACTCAGGAG
    CTTTTCCGAA AAGTGCGCAG TATTCTCAAT AAACTGACAC CGCAGATGTT CAGCCAGCTG
    ATGAAGCAGG TGACTGACCT CACCATCGAC ACAGAGGAGC GACTCAAGGG TGTCATAGAT
    CTGGTCTTTG AAAAAGCAAT CAATGAGCCC AGCTTCTCTG TGGCCTACGG CAACATGTGC
    AGCTGTCTGG CCACGTTAAA AGTGCCCATG ACGGATAAGC CCAACAGCAC TGTGAATTTC
    CGGAAGCTGC TTCTGAACCG CTGTCAGAAA GAGTTTGAGA AGGACAAGAT GGATGACGAT
    GCCTTTGAGA AGAAACACAG GGAGCTGGAG GCAGCTACTG CGTCCAGTGA GCGGGAGAGG
    CTTCAGGAGG AGCTGGAAGA GGCCAAAGAC AAGGCCCGAA GACGCTCCAT TGGCAACATT
    AAGTTCATCG GCGAGCTCTT CAAGCTGCGG ATGCTGACAG AGGCCATCAT GCATGACTGC
    GTGGTGAAGT TGCTGAAAAA CCATGATGAG GAATCACTGG AGTGCCTGTG TAGACTGCTC
    ACCACTATTG GAAAGGACCT GGACTTTGAG AAAGCTAAGC CACGAATGGA TCAGTACTTT
    AATCAGATGG AGAAGATAGT TAAGGAGAGG AAGACTTCTT CACGCATTCG CTTCATGCTG
    CAGGATGTTA TTGATCTGCG ATTGCACAAC TGGGTTTCTC GAAGAGCAGA TCAGGGCCCC
    AAGACCATCG AGCAGATCCA TAAAGACGCA AAGCTGGAGG AGCAGGAGGA ACAGAGGAAA
    GTCCACCAGC AACTGCTTTC CAAAGACAAC AAGAGGAGAC CAGTGGTGCA GAGAGAGGAG
    ACCTGGAGCA CTGTGCCTAT GACTAAGAAC AGCAGAACAA TAGACCCCGC CAAGATTCCC
    AAATTCTCCA AGCCAGCGAT AGATGAGAAG ATTCAGCTGG GGCCGAGAGC TCAGGTGAAC
    TGGATGAAGG GCAGCAGTGG TGGAGCTGGA GCAAAGGCCA GTGAGTCAGA TGCGTCTCGG
    CCGAGTGCCA GTTTGAATCG TTACTCTCCT CTGCAACCCT CGGCACTCCA GACTTCTTCT
    TTACCCTCCA CATCTCCAGA TTTTGATTCC AGGAGAGTTC TCGGCAGTCG TGGAAGCTCA
    GGACGGGAGC GCAATGACAA GCCATTGAGT GCGGGTCCTG CTCGGACAGG GCCTATTTCA
    TTGAGCAGTA GCAATAAGGA GACCCCCGAA GAGCTGGTTC AGGAAGTGTC CCGCAGAGAC
    TCCAATGCTT CAGATACACC CAAGCTGCTC GTCAGTACTG CAGACAAGAG CAGACTGGAG
    AACAGCCAAC CCAGAGAGTC TGCTGTTAAA CTTGAAGCAC TCTCAGGGCC CTCTCCAGAT
    AAACCTGCAT TATCAGAAGA GGAGATGGAG AGGCGGTCCA AGTCTATAAT TGATGAGTTT
    TTGCACATTA ATGATTACAA GGAAGCTTTG CAGTGTGTTG AGGAACTAGA GCAGTCTGCC
    ATGCTCTATG TGTTTGTGCG GGTGGGTGTG GAGTCCACGC TGGAAAGGAG TCAGATCACG
    CGAGACCATA TGGGTCAGCT GCTCTTCCAG CTTCTGCAGG CTGGAGTCCT CCCTAAACTT
    CAGTTCTTTA AAGGGTTTTC TGAAACTCTT GAATTAGCAG ATGATATGGC CATTGATATT
    CCCCATATAT GGCTGTACCT TGCGGAGCTG GTAACCCCTG TGCTTCGAGA GGGGGGAATC
    TCTATGAGAG AATTATTTAG TGAATTTAGC AAACCATTAC TCCCTGTGGG AAGAGCTGGG
    ATATTATTTT CTGAAATATT GCACCTTCTA TGCAAACAAA TGAGCCATAA GAAAGTGGGA
    TCATTATGGA GGGATTCTGG GTTGAGCTGG GCAGATTTTA TACCTGAAAC AGAGGATGTG
    CACAGCTTCA TCACAGAACA TAAACTAGAG TTCACTCTGT CTGATGGCTC AAACCCGACC
    GAGGCTGTCT CTAAGAGAAC ACTCTCGCCT GAGGAACTGA ACAAGCAACT TGAAAAACTT
    TTACTTGAGG ACATGGTTGG CGATGAACAA ATCTTCGACT GGGTTGAGGC CAATCTAGAT
    GAATCAGAAA TGAGTTCAGC TCCTTTCGTC AGAGCTTTAA TGACTGCTGT GTGTAAAGCA
    GCAGTAAAAA CTGAGGGCTC CTCGTGTAAA GTGGACTTGT CCATAATTCA GACGAGATTG
    CCTGTATTAC ACAAGTACCT TAACTCAGAC ACAGAGAGAC AGTTGCAAGC ACTTTATGCA
    CTTCAAGCAT TGATAGTAAA ACTGGACCAG CCTGCTAATT TGCTCCGGAT GTTTTTTGAC
    TGTTTGTATG ATGAGGACGT GATCTCTGAA GACGCTTTCT ATAAATGGGA GGTCAGTAAA
    GACCCCGCTG AACAGCAAGG CAAAGGTGTT GCCCTGAAGT CCGTCACAGC CTTCTTCACG
    TGGCTGCGCG AGGCCGAGGA AGAGTCCGAG GACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa151558
    Clone ID Related Accession (Same CDS sequence) XM_021469955.1
    Accession Version XM_021469955.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5181bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACTAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CAACAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTGCCCCT GGGCCTCGTC CTACCCATCA CCAGTTTTTC
    CAGAGGACGC AGATGCAAAC GGCCCGGCCC ACCATTCCAA GCAACACCCC ACCCATTCGG
    CCCACGTCTC AGACCCCCAC GGCAGCAGTA TATTCTCCCA ATCAGCACAT CATGATGACC
    ATGGCTCACA TGCCCTTTCA TTCCCCACAG ACAGCGCAGT ACTACATCCC TCAGTATCGT
    CACAGTGCTC CACAGTATGT GGGACCTCCT CAACAGTACC CTGTCCAGCC GACTGGACCC
    AGTACCTTCT ATGCTGCAGC CAGTCCAGGG GAGTTTCCTG CACCTTATGC AGCAGGACCT
    CCCTACTATC CTGGGCAGCC CGTGTACACA CCATCTCCAC CCATTATAGT GCCTACACCA
    CAACAACCTC CACCTGCTAA GAGGGAAAAG AAAACAATCC GCATCCGAGA CCCTAACCAG
    GGTGGCAAGG ATGTCACAGA CGAAATATTA TCAGGAGTGG GTTTGAGTCG AAACCCAACC
    CCGCCTGTGG GACGGCCCTC TTCCACTCCT ACACCTCCAC AGTTTTTATG TCCACACCCT
    CATTATCCTC ACATTTTCTA CCTCAAATCC CAGCAGCTGA ACAGCCAGGT AGCAGATCAT
    GGGCACATCA TGTATAATGT GGACAGCAGC CCGCACCTTC CAGCATCCTT CAACTTGAAA
    GCAGACGATA AGCCAAAACT AGAGTTTTCC TTGCAAAGAA CTGCATCTCC TGGTCTGCGG
    CAGCCCGACA CTCCTCTGGA GAGAAGAGAT CCCAGCAGCC CAGTCCAAAC ACCCTCCTCT
    CCCCCACACA AACCTGAGCT TCCCCCCTCC GACTCAGAGA CGGCCTCAAG TGTAGCTACA
    GCCCCGACTC CTTCCATACC TGCCTCAACA GAAGAGTCAG CTGATGCCCC GAGCCCATTA
    GCTGAGCCCA GTCTCACTAA AGCTATCACA CCTGAACCAG AGAGCTCTGA GCCAGAGAAA
    TCCTCCTCAC CACCACCTCA AAGTCTGTCT GGATCCCTTA CCCAGCACGA AAAAGCCGTC
    AATGGGCTCA CGGATGTTGA CGCTGCTCCA CTGAGTGAAG AATTGGAAAC CCAACCCAGG
    GAAGCCTCTC CATTGCTGCC TACTTCCAGT GTGCCCCAGT CAGAGCCCAG GCCAGTCACA
    CCGGTGCTGG AGGAAGAGTC TGATCCAATC AATATGAACA GTCCTTTGCC TCCTGTTGAG
    GACGATGCTG GATGTCCTGA CAACGTTTCC CCCTCCCTCT CCACCTCCAC GACTGCTGCT
    ATCTCCACCA CTCCTCCTGC TCCTCCTCCA GGACTCTCGC ACCCCAGTCA GGTTTCTGCA
    GCGTTGGACA GGAGACCCTC TAATGGAGCA GAGATCAAAG AAACAGGGAA GGAAAACGAG
    GCCTTGCCGG ACAAGAGAGG AGAGCCTTTC TTACAGTCCA GAAAAAGCTC AAATCAAGCT
    ACATCTAGTG CACCAAAGAC CTGGAAGAAG CCAAAAGAGG ACATGCCTGT AGGGCAAGAT
    CAGTGTCCAG ACAAAGAGGA GGACACTGAT CAGGCCAGTA TGGAACCTGC TCCTGAAAGC
    CCCAGTCCTC CTCCTGTGGA GGTGGAGAGG AGGCCATCAG TAGGAGCAGT GCTCGAGGAG
    AATGGAGAAC AGGAAGTGGA GCCTTTGAGA AACGGTGCTG AACACACTTC TGAAACTGAG
    AGCAGTGACA GCATCCACCC GCCTGCAGTC AAAGAGCCGT TGATCAAAGT ATTGCCACAG
    CCTGCAGAGA AGAATGGAAA GAAGCAGTAC AACAGAGACT TCCTGCTGGG CTTCCAGTTT
    ATGCCTGCAT GTGTGCAGAA ACCAGAAGGG CTTCCTCCCA TTTCTGATGT TGTTCTAGAT
    AAGATTAACC AAAATAAGCT GCCCATGCGG CCGGTAGACC CACGTGTGCT TTCCAGAGGC
    CCGGACTTTA CGCCAGCGTT TGCGGATTTC AGCCGACCCA TTTCAGGAGG CAGAGGGGCA
    CCGTTGCTGA ATGTTGGCAT GCGACGGCCA CCTGGACGCA AGATCATCAC AAACGTGTCT
    GTGATTGATG AGGTGCAGCT GAAGACAACA GAGAATGCCT GGAAGCCTGG CATGAAGCGG
    GAAGGTGCAG TTGAAGATCC AGATGCCCAG AGAACTCAGG AGCTTTTCCG AAAAGTGCGC
    AGTATTCTCA ATAAACTGAC ACCGCAGATG TTCAGCCAGC TGATGAAGCA GGTGACTGAC
    CTCACCATCG ACACAGAGGA GCGACTCAAG GGTGTCATAG ATCTGGTCTT TGAAAAAGCA
    ATCAATGAGC CCAGCTTCTC TGTGGCCTAC GGCAACATGT GCAGCTGTCT GGCCACGTTA
    AAAGTGCCCA TGACGGATAA GCCCAACAGC ACTGTGAATT TCCGGAAGCT GCTTCTGAAC
    CGCTGTCAGA AAGAGTTTGA GAAGGACAAG ATGGATGACG ATGCCTTTGA GAAGAAACAC
    AGGGAGCTGG AGGCAGCTAC TGCGTCCAGT GAGCGGGAGA GGCTTCAGGA GGAGCTGGAA
    GAGGCCAAAG ACAAGGCCCG AAGACGCTCC ATTGGCAACA TTAAGTTCAT CGGCGAGCTC
    TTCAAGCTGC GGATGCTGAC AGAGGCCATC ATGCATGACT GCGTGGTGAA GTTGCTGAAA
    AACCATGATG AGGAATCACT GGAGTGCCTG TGTAGACTGC TCACCACTAT TGGAAAGGAC
    CTGGACTTTG AGAAAGCTAA GCCACGAATG GATCAGTACT TTAATCAGAT GGAGAAGATA
    GTTAAGGAGA GGAAGACTTC TTCACGCATT CGCTTCATGC TGCAGGATGT TATTGATCTG
    CGATTGCACA ACTGGGTTTC TCGAAGAGCA GATCAGGGCC CCAAGACCAT CGAGCAGATC
    CATAAAGACG CAAAGCTGGA GGAGCAGGAG GAACAGAGGA AAGTCCACCA GCAACTGCTT
    TCCAAAGACA ACAAGAGGAG ACCAGTGGTG CAGAGAGAGG AGACCTGGAG CACTGTGCCT
    ATGACTAAGA ACAGCAGAAC AATAGACCCC GCCAAGATTC CCAAATTCTC CAAGCCAGCG
    ATAGATGAGA AGATTCAGCT GGGGCCGAGA GCTCAGGTGA ACTGGATGAA GGGCAGCAGT
    GGTGGAGCTG GAGCAAAGGC CAGTGAGTCA GATGCGTCTC GGCCGAGTGC CAGTTTGAAT
    CGTTACTCTC CTCTGCAACC CTCGGCACTC CAGACTTCTT CTTTACCCTC CACATCTCCA
    GATTTTGATT CCAGGAGAGT TCTCGGCAGT CGTGGAAGCT CAGGACGGGA GCGCAATGAC
    AAGCCATTGA GTGCGGGTCC TGCTCGGACA GGGCCTATTT CATTGAGCAG TAGCAATAAG
    GAGACCCCCG AAGAGCTGGT TCAGGAAGTG TCCCGCAGAG ACTCCAATGC TTCAGATACA
    CCCAAGCTGC TCGTCAGTAC TGCAGACAAG AGCAGACTGG AGAACAGCCA ACCCAGAGAG
    TCTGCTGTTA AACTTGAAGC ACTCTCAGGG CCCTCTCCAG ATAAACCTGC ATTATCAGAA
    GAGGAGATGG AGAGGCGGTC CAAGTCTATA ATTGATGAGT TTTTGCACAT TAATGATTAC
    AAGGAAGCTT TGCAGTGTGT TGAGGAACTA GAGCAGTCTG CCATGCTCTA TGTGTTTGTG
    CGGGTGGGTG TGGAGTCCAC GCTGGAAAGG AGTCAGATCA CGCGAGACCA TATGGGTCAG
    CTGCTCTTCC AGCTTCTGCA GGCTGGAGTC CTCCCTAAAC TTCAGTTCTT TAAAGGGTTT
    TCTGAAACTC TTGAATTAGC AGATGATATG GCCATTGATA TTCCCCATAT ATGGCTGTAC
    CTTGCGGAGC TGGTAACCCC TGTGCTTCGA GAGGGGGGAA TCTCTATGAG AGAATTATTT
    AGTGAATTTA GCAAACCATT ACTCCCTGTG GGAAGAGCTG GGATATTATT TTCTGAAATA
    TTGCACCTTC TATGCAAACA AATGAGCCAT AAGAAAGTGG GATCATTATG GAGGGATTCT
    GGGTTGAGCT GGGCAGATTT TATACCTGAA ACAGAGGATG TGCACAGCTT CATCACAGAA
    CATAAACTAG AGTTCACTCT GTCTGATGGC TCAAACCCGA CCGAGGCTGT CTCTAAGAGA
    ACACTCTCGC CTGAGGAACT GAACAAGCAA CTTGAAAAAC TTTTACTTGA GGACATGGTT
    GGCGATGAAC AAATCTTCGA CTGGGTTGAG GCCAATCTAG ATGAATCAGA AATGAGTTCA
    GCTCCTTTCG TCAGAGCTTT AATGACTGCT GTGTGTAAAG CAGCAGTAAA AACTGAGGGC
    TCCTCGTGTA AAGTGGACTT GTCCATAATT CAGACGAGAT TGCCTGTATT ACACAAGTAC
    CTTAACTCAG ACACAGAGAG ACAGTTGCAA GCACTTTATG CACTTCAAGC ATTGATAGTA
    AAACTGGACC AGCCTGCTAA TTTGCTCCGG ATGTTTTTTG ACTGTTTGTA TGATGAGGAC
    GTGATCTCTG AAGACGCTTT CTATAAATGG GAGGTCAGTA AAGACCCCGC TGAACAGCAA
    GGCAAAGGTG TTGCCCTGAA GTCCGTCACA GCCTTCTTCA CGTGGCTGCG CGAGGCCGAG
    GAAGAGTCCG AGGACAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325630.1
    CDS458..5638
    Translation

    Target ORF information:

    RefSeq Version XM_021469955.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469955.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACTAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CAACAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTGCCCCT GGGCCTCGTC CTACCCATCA CCAGTTTTTC
    CAGAGGACGC AGATGCAAAC GGCCCGGCCC ACCATTCCAA GCAACACCCC ACCCATTCGG
    CCCACGTCTC AGACCCCCAC GGCAGCAGTA TATTCTCCCA ATCAGCACAT CATGATGACC
    ATGGCTCACA TGCCCTTTCA TTCCCCACAG ACAGCGCAGT ACTACATCCC TCAGTATCGT
    CACAGTGCTC CACAGTATGT GGGACCTCCT CAACAGTACC CTGTCCAGCC GACTGGACCC
    AGTACCTTCT ATGCTGCAGC CAGTCCAGGG GAGTTTCCTG CACCTTATGC AGCAGGACCT
    CCCTACTATC CTGGGCAGCC CGTGTACACA CCATCTCCAC CCATTATAGT GCCTACACCA
    CAACAACCTC CACCTGCTAA GAGGGAAAAG AAAACAATCC GCATCCGAGA CCCTAACCAG
    GGTGGCAAGG ATGTCACAGA CGAAATATTA TCAGGAGTGG GTTTGAGTCG AAACCCAACC
    CCGCCTGTGG GACGGCCCTC TTCCACTCCT ACACCTCCAC AGTTTTTATG TCCACACCCT
    CATTATCCTC ACATTTTCTA CCTCAAATCC CAGCAGCTGA ACAGCCAGGT AGCAGATCAT
    GGGCACATCA TGTATAATGT GGACAGCAGC CCGCACCTTC CAGCATCCTT CAACTTGAAA
    GCAGACGATA AGCCAAAACT AGAGTTTTCC TTGCAAAGAA CTGCATCTCC TGGTCTGCGG
    CAGCCCGACA CTCCTCTGGA GAGAAGAGAT CCCAGCAGCC CAGTCCAAAC ACCCTCCTCT
    CCCCCACACA AACCTGAGCT TCCCCCCTCC GACTCAGAGA CGGCCTCAAG TGTAGCTACA
    GCCCCGACTC CTTCCATACC TGCCTCAACA GAAGAGTCAG CTGATGCCCC GAGCCCATTA
    GCTGAGCCCA GTCTCACTAA AGCTATCACA CCTGAACCAG AGAGCTCTGA GCCAGAGAAA
    TCCTCCTCAC CACCACCTCA AAGTCTGTCT GGATCCCTTA CCCAGCACGA AAAAGCCGTC
    AATGGGCTCA CGGATGTTGA CGCTGCTCCA CTGAGTGAAG AATTGGAAAC CCAACCCAGG
    GAAGCCTCTC CATTGCTGCC TACTTCCAGT GTGCCCCAGT CAGAGCCCAG GCCAGTCACA
    CCGGTGCTGG AGGAAGAGTC TGATCCAATC AATATGAACA GTCCTTTGCC TCCTGTTGAG
    GACGATGCTG GATGTCCTGA CAACGTTTCC CCCTCCCTCT CCACCTCCAC GACTGCTGCT
    ATCTCCACCA CTCCTCCTGC TCCTCCTCCA GGACTCTCGC ACCCCAGTCA GGTTTCTGCA
    GCGTTGGACA GGAGACCCTC TAATGGAGCA GAGATCAAAG AAACAGGGAA GGAAAACGAG
    GCCTTGCCGG ACAAGAGAGG AGAGCCTTTC TTACAGTCCA GAAAAAGCTC AAATCAAGCT
    ACATCTAGTG CACCAAAGAC CTGGAAGAAG CCAAAAGAGG ACATGCCTGT AGGGCAAGAT
    CAGTGTCCAG ACAAAGAGGA GGACACTGAT CAGGCCAGTA TGGAACCTGC TCCTGAAAGC
    CCCAGTCCTC CTCCTGTGGA GGTGGAGAGG AGGCCATCAG TAGGAGCAGT GCTCGAGGAG
    AATGGAGAAC AGGAAGTGGA GCCTTTGAGA AACGGTGCTG AACACACTTC TGAAACTGAG
    AGCAGTGACA GCATCCACCC GCCTGCAGTC AAAGAGCCGT TGATCAAAGT ATTGCCACAG
    CCTGCAGAGA AGAATGGAAA GAAGCAGTAC AACAGAGACT TCCTGCTGGG CTTCCAGTTT
    ATGCCTGCAT GTGTGCAGAA ACCAGAAGGG CTTCCTCCCA TTTCTGATGT TGTTCTAGAT
    AAGATTAACC AAAATAAGCT GCCCATGCGG CCGGTAGACC CACGTGTGCT TTCCAGAGGC
    CCGGACTTTA CGCCAGCGTT TGCGGATTTC AGCCGACCCA TTTCAGGAGG CAGAGGGGCA
    CCGTTGCTGA ATGTTGGCAT GCGACGGCCA CCTGGACGCA AGATCATCAC AAACGTGTCT
    GTGATTGATG AGGTGCAGCT GAAGACAACA GAGAATGCCT GGAAGCCTGG CATGAAGCGG
    GAAGGTGCAG TTGAAGATCC AGATGCCCAG AGAACTCAGG AGCTTTTCCG AAAAGTGCGC
    AGTATTCTCA ATAAACTGAC ACCGCAGATG TTCAGCCAGC TGATGAAGCA GGTGACTGAC
    CTCACCATCG ACACAGAGGA GCGACTCAAG GGTGTCATAG ATCTGGTCTT TGAAAAAGCA
    ATCAATGAGC CCAGCTTCTC TGTGGCCTAC GGCAACATGT GCAGCTGTCT GGCCACGTTA
    AAAGTGCCCA TGACGGATAA GCCCAACAGC ACTGTGAATT TCCGGAAGCT GCTTCTGAAC
    CGCTGTCAGA AAGAGTTTGA GAAGGACAAG ATGGATGACG ATGCCTTTGA GAAGAAACAC
    AGGGAGCTGG AGGCAGCTAC TGCGTCCAGT GAGCGGGAGA GGCTTCAGGA GGAGCTGGAA
    GAGGCCAAAG ACAAGGCCCG AAGACGCTCC ATTGGCAACA TTAAGTTCAT CGGCGAGCTC
    TTCAAGCTGC GGATGCTGAC AGAGGCCATC ATGCATGACT GCGTGGTGAA GTTGCTGAAA
    AACCATGATG AGGAATCACT GGAGTGCCTG TGTAGACTGC TCACCACTAT TGGAAAGGAC
    CTGGACTTTG AGAAAGCTAA GCCACGAATG GATCAGTACT TTAATCAGAT GGAGAAGATA
    GTTAAGGAGA GGAAGACTTC TTCACGCATT CGCTTCATGC TGCAGGATGT TATTGATCTG
    CGATTGCACA ACTGGGTTTC TCGAAGAGCA GATCAGGGCC CCAAGACCAT CGAGCAGATC
    CATAAAGACG CAAAGCTGGA GGAGCAGGAG GAACAGAGGA AAGTCCACCA GCAACTGCTT
    TCCAAAGACA ACAAGAGGAG ACCAGTGGTG CAGAGAGAGG AGACCTGGAG CACTGTGCCT
    ATGACTAAGA ACAGCAGAAC AATAGACCCC GCCAAGATTC CCAAATTCTC CAAGCCAGCG
    ATAGATGAGA AGATTCAGCT GGGGCCGAGA GCTCAGGTGA ACTGGATGAA GGGCAGCAGT
    GGTGGAGCTG GAGCAAAGGC CAGTGAGTCA GATGCGTCTC GGCCGAGTGC CAGTTTGAAT
    CGTTACTCTC CTCTGCAACC CTCGGCACTC CAGACTTCTT CTTTACCCTC CACATCTCCA
    GATTTTGATT CCAGGAGAGT TCTCGGCAGT CGTGGAAGCT CAGGACGGGA GCGCAATGAC
    AAGCCATTGA GTGCGGGTCC TGCTCGGACA GGGCCTATTT CATTGAGCAG TAGCAATAAG
    GAGACCCCCG AAGAGCTGGT TCAGGAAGTG TCCCGCAGAG ACTCCAATGC TTCAGATACA
    CCCAAGCTGC TCGTCAGTAC TGCAGACAAG AGCAGACTGG AGAACAGCCA ACCCAGAGAG
    TCTGCTGTTA AACTTGAAGC ACTCTCAGGG CCCTCTCCAG ATAAACCTGC ATTATCAGAA
    GAGGAGATGG AGAGGCGGTC CAAGTCTATA ATTGATGAGT TTTTGCACAT TAATGATTAC
    AAGGAAGCTT TGCAGTGTGT TGAGGAACTA GAGCAGTCTG CCATGCTCTA TGTGTTTGTG
    CGGGTGGGTG TGGAGTCCAC GCTGGAAAGG AGTCAGATCA CGCGAGACCA TATGGGTCAG
    CTGCTCTTCC AGCTTCTGCA GGCTGGAGTC CTCCCTAAAC TTCAGTTCTT TAAAGGGTTT
    TCTGAAACTC TTGAATTAGC AGATGATATG GCCATTGATA TTCCCCATAT ATGGCTGTAC
    CTTGCGGAGC TGGTAACCCC TGTGCTTCGA GAGGGGGGAA TCTCTATGAG AGAATTATTT
    AGTGAATTTA GCAAACCATT ACTCCCTGTG GGAAGAGCTG GGATATTATT TTCTGAAATA
    TTGCACCTTC TATGCAAACA AATGAGCCAT AAGAAAGTGG GATCATTATG GAGGGATTCT
    GGGTTGAGCT GGGCAGATTT TATACCTGAA ACAGAGGATG TGCACAGCTT CATCACAGAA
    CATAAACTAG AGTTCACTCT GTCTGATGGC TCAAACCCGA CCGAGGCTGT CTCTAAGAGA
    ACACTCTCGC CTGAGGAACT GAACAAGCAA CTTGAAAAAC TTTTACTTGA GGACATGGTT
    GGCGATGAAC AAATCTTCGA CTGGGTTGAG GCCAATCTAG ATGAATCAGA AATGAGTTCA
    GCTCCTTTCG TCAGAGCTTT AATGACTGCT GTGTGTAAAG CAGCAGTAAA AACTGAGGGC
    TCCTCGTGTA AAGTGGACTT GTCCATAATT CAGACGAGAT TGCCTGTATT ACACAAGTAC
    CTTAACTCAG ACACAGAGAG ACAGTTGCAA GCACTTTATG CACTTCAAGC ATTGATAGTA
    AAACTGGACC AGCCTGCTAA TTTGCTCCGG ATGTTTTTTG ACTGTTTGTA TGATGAGGAC
    GTGATCTCTG AAGACGCTTT CTATAAATGG GAGGTCAGTA AAGACCCCGC TGAACAGCAA
    GGCAAAGGTG TTGCCCTGAA GTCCGTCACA GCCTTCTTCA CGTGGCTGCG CGAGGCCGAG
    GAAGAGTCCG AGGACAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa151559
    Clone ID Related Accession (Same CDS sequence) XM_021469956.1
    Accession Version XM_021469956.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACTAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAATA TCCACGAGTG CCGCCTCCCC TGGACGAGCG AATCTTCTCA
    ACACAGCCTG TCTCTGCTGT CTACAGTGTA CAGAGACCCC CTGGCCCCCC ATTTACTGCA
    CATGAAATCA ACAAGGGACA CCCTAACCTA GCGGCTACCC CGCCGGGTCA TGCCTCTTCC
    CCTGGACTAT CTCAGGTATC AGTTTCAACA GTATCCACTG CGCATCTCTA TGGTCATCCT
    AAAGGCTGGG AGCCAGGAGG AGGGTCTCCG TACACAACGG GACAGAATGC CGGCACGACC
    CCTCTGGTAT ACTCTCCAAC AACCCAGCCA ATGAACGCAC AGCCTCAGAG CCGCCCGTTT
    GCCCCTGGGC CTCGTCCTAC CCATCACCAG GGAGGATTCA GATCCATTCA GTTTTTCCAG
    AGGACGCAGA TGCAAACGGC CCGGCCCACC ATTCCAAGCA ACACCCCACC CATTCGGCCC
    ACGTCTCAGA CCCCCACGGC AGCAGTATAT TCTCCCAATC AGCACATCAT GATGACCATG
    GCTCACATGC CCTTTCATTC CCCACAGACA GCGCAGTACT ACATCCCTCA GTATCGTCAC
    AGTGCTCCAC AGTATGTGGG ACCTCCTCAA CAGTACCCTG TCCAGCCGAC TGGACCCAGT
    ACCTTCTATG CTGCAGCCAG TCCAGGGGAG TTTCCTGCAC CTTATGCAGC AGGACCTCCC
    TACTATCCTG GGCAGCCCGT GTACACACCA TCTCCACCCA TTATAGTGCC TACACCACAA
    CAACCTCCAC CTGCTAAGAG GGAAAAGAAA ACAATCCGCA TCCGAGACCC TAACCAGGGT
    GGCAAGGATG TCACAGACGA AATATTATCA GGAGTGGGTT TGAGTCGAAA CCCAACCCCG
    CCTGTGGGAC GGCCCTCTTC CACTCCTACA CCTCCACAGT TTTTATGTCC ACACCCTCAT
    TATCCTCACA TTTTCTACCT CAAATCCCAG CAGCTGAACA GCCAGGTAGC AGATCATGGG
    CACATCATGT ATAATGTGGA CAGCAGCCCG CACCTTCCAG CATCCTTCAA CTTGAAAGCA
    GACGATAAGC CAAAACTAGA GTTTTCCTTG CAAAGAACTG CATCTCCTGG TCTGCGGCAG
    CCCGACACTC CTCTGGAGAG AAGAGATCCC AGCAGCCCAG TCCAAACACC CTCCTCTCCC
    CCACACAAAC CTGAGCTTCC CCCCTCCGAC TCAGAGACGG CCTCAAGTGT AGCTACAGCC
    CCGACTCCTT CCATACCTGC CTCAACAGAA GAGTCAGCTG ATGCCCCGAG CCCATTAGCT
    GAGCCCAGTC TCACTAAAGC TATCACACCT GAACCAGAGA GCTCTGAGCC AGAGAAATCC
    TCCTCACCAC CACCTCAAAG TCTGTCTGGA TCCCTTACCC AGCACGAAAA AGCCGTCAAT
    GGGCTCACGG ATGTTGACGC TGCTCCACTG AGTGAAGAAT TGGAAACCCA ACCCAGGGAA
    GCCTCTCCAT TGCTGCCTAC TTCCAGTGTG CCCCAGTCAG AGCCCAGGCC AGTCACACCG
    GTGCTGGAGG AAGAGTCTGA TCCAATCAAT ATGAACAGTC CTTTGCCTCC TGTTGAGGAC
    GATGCTGGAT GTCCTGACAA CGTTTCCCCC TCCCTCTCCA CCTCCACGAC TGCTGCTATC
    TCCACCACTC CTCCTGCTCC TCCTCCAGGA CTCTCGCACC CCAGTCAGGT TTCTGCAGCG
    TTGGACAGGA GACCCTCTAA TGGAGCAGAG ATCAAAGAAA CAGGGAAGGA AAACGAGGCC
    TTGCCGGACA AGAGAGGAGA GCCTTTCTTA CAGTCCAGAA AAAGCTCAAA TCAAGCTACA
    TCTAGTGCAC CAAAGACCTG GAAGAAGCCA AAAGAGGACA TGCCTGTAGG GCAAGATCAG
    TGTCCAGACA AAGAGGAGGA CACTGATCAG GCCAGTATGG AACCTGCTCC TGAAAGCCCC
    AGTCCTCCTC CTGTGGAGGT GGAGAGGAGG CCATCAGTAG GAGCAGTGCT CGAGGAGAAT
    GGAGAACAGG AAGTGGAGCC TTTGAGAAAC GGTGCTGAAC ACACTTCTGA AACTGAGAGC
    AGTGACAGCA TCCACCCGCC TGCAGTCAAA GAGCCGTTGA TCAAAGTATT GCCACAGCCT
    GCAGAGAAGA ATGGAAAGAA GCAGTACAAC AGAGACTTCC TGCTGGGCTT CCAGTTTATG
    CCTGCATGTG TGCAGAAACC AGAAGGGCTT CCTCCCATTT CTGATGTTGT TCTAGATAAG
    ATTAACCAAA ATAAGCTGCC CATGCGGCCG GTAGACCCAC GTGTGCTTTC CAGAGGCCCG
    GACTTTACGC CAGCGTTTGC GGATTTCAGC CGACCCATTT CAGGAGGCAG AGGGGCACCG
    TTGCTGAATG TTGGCATGCG ACGGCCACCT GGACGCAAGA TCATCACAAA CGTGTCTGTG
    ATTGATGAGG TGCAGCTGAA GACAACAGAG AATGCCTGGA AGCCTGGCAT GAAGCGGGAA
    GGTGCAGTTG AAGATCCAGA TGCCCAGAGA ACTCAGGAGC TTTTCCGAAA AGTGCGCAGT
    ATTCTCAATA AACTGACACC GCAGATGTTC AGCCAGCTGA TGAAGCAGGT GACTGACCTC
    ACCATCGACA CAGAGGAGCG ACTCAAGGGT GTCATAGATC TGGTCTTTGA AAAAGCAATC
    AATGAGCCCA GCTTCTCTGT GGCCTACGGC AACATGTGCA GCTGTCTGGC CACGTTAAAA
    GTGCCCATGA CGGATAAGCC CAACAGCACT GTGAATTTCC GGAAGCTGCT TCTGAACCGC
    TGTCAGAAAG AGTTTGAGAA GGACAAGATG GATGACGATG CCTTTGAGAA GAAACACAGG
    GAGCTGGAGG CAGCTACTGC GTCCAGTGAG CGGGAGAGGC TTCAGGAGGA GCTGGAAGAG
    GCCAAAGACA AGGCCCGAAG ACGCTCCATT GGCAACATTA AGTTCATCGG CGAGCTCTTC
    AAGCTGCGGA TGCTGACAGA GGCCATCATG CATGACTGCG TGGTGAAGTT GCTGAAAAAC
    CATGATGAGG AATCACTGGA GTGCCTGTGT AGACTGCTCA CCACTATTGG AAAGGACCTG
    GACTTTGAGA AAGCTAAGCC ACGAATGGAT CAGTACTTTA ATCAGATGGA GAAGATAGTT
    AAGGAGAGGA AGACTTCTTC ACGCATTCGC TTCATGCTGC AGGATGTTAT TGATCTGCGA
    TTGCACAACT GGGTTTCTCG AAGAGCAGAT CAGGGCCCCA AGACCATCGA GCAGATCCAT
    AAAGACGCAA AGCTGGAGGA GCAGGAGGAA CAGAGGAAAG TCCACCAGCA ACTGCTTTCC
    AAAGACAACA AGAGGAGACC AGTGGTGCAG AGAGAGGAGA CCTGGAGCAC TGTGCCTATG
    ACTAAGAACA GCAGAACAAT AGACCCCGCC AAGATTCCCA AATTCTCCAA GCCAGCGATA
    GATGAGAAGA TTCAGCTGGG GCCGAGAGCT CAGGTGAACT GGATGAAGGG CAGCAGTGGT
    GGAGCTGGAG CAAAGGCCAG TGAGTCAGAT GCGTCTCGGC CGAGTGCCAG TTTGAATCGT
    TACTCTCCTC TGCAACCCTC GGCACTCCAG ACTTCTTCTT TACCCTCCAC ATCTCCAGAT
    TTTGATTCCA GGAGAGTTCT CGGCAGTCGT GGAAGCTCAG GACGGGAGCG CAATGACAAG
    CCATTGAGTG CGGGTCCTGC TCGGACAGGG CCTATTTCAT TGAGCAGTAG CAATAAGGAG
    ACCCCCGAAG AGCTGGTTCA GGAAGTGTCC CGCAGAGACT CCAATGCTTC AGATACACCC
    AAGCTGCTCG TCAGTACTGC AGACAAGAGC AGACTGGAGA ACAGCCAACC CAGAGAGTCT
    GCTGTTAAAC TTGAAGCACT CTCAGGGCCC TCTCCAGATA AACCTGCATT ATCAGAAGAG
    GAGATGGAGA GGCGGTCCAA GTCTATAATT GATGAGTTTT TGCACATTAA TGATTACAAG
    GAAGCTTTGC AGTGTGTTGA GGAACTAGAG CAGTCTGCCA TGCTCTATGT GTTTGTGCGG
    GTGGGTGTGG AGTCCACGCT GGAAAGGAGT CAGATCACGC GAGACCATAT GGGTCAGCTG
    CTCTTCCAGC TTCTGCAGGC TGGAGTCCTC CCTAAACTTC AGTTCTTTAA AGGGTTTTCT
    GAAACTCTTG AATTAGCAGA TGATATGGCC ATTGATATTC CCCATATATG GCTGTACCTT
    GCGGAGCTGG TAACCCCTGT GCTTCGAGAG GGGGGAATCT CTATGAGAGA ATTATTTAGT
    GAATTTAGCA AACCATTACT CCCTGTGGGA AGAGCTGGGA TATTATTTTC TGAAATATTG
    CACCTTCTAT GCAAACAAAT GAGCCATAAG AAAGTGGGAT CATTATGGAG GGATTCTGGG
    TTGAGCTGGG CAGATTTTAT ACCTGAAACA GAGGATGTGC ACAGCTTCAT CACAGAACAT
    AAACTAGAGT TCACTCTGTC TGATGGCTCA AACCCGACCG AGGCTGTCTC TAAGAGAACA
    CTCTCGCCTG AGGAACTGAA CAAGCAACTT GAAAAACTTT TACTTGAGGA CATGGTTGGC
    GATGAACAAA TCTTCGACTG GGTTGAGGCC AATCTAGATG AATCAGAAAT GAGTTCAGCT
    CCTTTCGTCA GAGCTTTAAT GACTGCTGTG TGTAAAGCAG CAGTAAAAAC TGAGGGCTCC
    TCGTGTAAAG TGGACTTGTC CATAATTCAG ACGAGATTGC CTGTATTACA CAAGTACCTT
    AACTCAGACA CAGAGAGACA GTTGCAAGCA CTTTATGCAC TTCAAGCATT GATAGTAAAA
    CTGGACCAGC CTGCTAATTT GCTCCGGATG TTTTTTGACT GTTTGTATGA TGAGGACGTG
    ATCTCTGAAG ACGCTTTCTA TAAATGGGAG GTCAGTAAAG ACCCCGCTGA ACAGCAAGGC
    AAAGGTGTTG CCCTGAAGTC CGTCACAGCC TTCTTCACGT GGCTGCGCGA GGCCGAGGAA
    GAGTCCGAGG ACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325631.1
    CDS458..5635
    Translation

    Target ORF information:

    RefSeq Version XM_021469956.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469956.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACTAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAATA TCCACGAGTG CCGCCTCCCC TGGACGAGCG AATCTTCTCA
    ACACAGCCTG TCTCTGCTGT CTACAGTGTA CAGAGACCCC CTGGCCCCCC ATTTACTGCA
    CATGAAATCA ACAAGGGACA CCCTAACCTA GCGGCTACCC CGCCGGGTCA TGCCTCTTCC
    CCTGGACTAT CTCAGGTATC AGTTTCAACA GTATCCACTG CGCATCTCTA TGGTCATCCT
    AAAGGCTGGG AGCCAGGAGG AGGGTCTCCG TACACAACGG GACAGAATGC CGGCACGACC
    CCTCTGGTAT ACTCTCCAAC AACCCAGCCA ATGAACGCAC AGCCTCAGAG CCGCCCGTTT
    GCCCCTGGGC CTCGTCCTAC CCATCACCAG GGAGGATTCA GATCCATTCA GTTTTTCCAG
    AGGACGCAGA TGCAAACGGC CCGGCCCACC ATTCCAAGCA ACACCCCACC CATTCGGCCC
    ACGTCTCAGA CCCCCACGGC AGCAGTATAT TCTCCCAATC AGCACATCAT GATGACCATG
    GCTCACATGC CCTTTCATTC CCCACAGACA GCGCAGTACT ACATCCCTCA GTATCGTCAC
    AGTGCTCCAC AGTATGTGGG ACCTCCTCAA CAGTACCCTG TCCAGCCGAC TGGACCCAGT
    ACCTTCTATG CTGCAGCCAG TCCAGGGGAG TTTCCTGCAC CTTATGCAGC AGGACCTCCC
    TACTATCCTG GGCAGCCCGT GTACACACCA TCTCCACCCA TTATAGTGCC TACACCACAA
    CAACCTCCAC CTGCTAAGAG GGAAAAGAAA ACAATCCGCA TCCGAGACCC TAACCAGGGT
    GGCAAGGATG TCACAGACGA AATATTATCA GGAGTGGGTT TGAGTCGAAA CCCAACCCCG
    CCTGTGGGAC GGCCCTCTTC CACTCCTACA CCTCCACAGT TTTTATGTCC ACACCCTCAT
    TATCCTCACA TTTTCTACCT CAAATCCCAG CAGCTGAACA GCCAGGTAGC AGATCATGGG
    CACATCATGT ATAATGTGGA CAGCAGCCCG CACCTTCCAG CATCCTTCAA CTTGAAAGCA
    GACGATAAGC CAAAACTAGA GTTTTCCTTG CAAAGAACTG CATCTCCTGG TCTGCGGCAG
    CCCGACACTC CTCTGGAGAG AAGAGATCCC AGCAGCCCAG TCCAAACACC CTCCTCTCCC
    CCACACAAAC CTGAGCTTCC CCCCTCCGAC TCAGAGACGG CCTCAAGTGT AGCTACAGCC
    CCGACTCCTT CCATACCTGC CTCAACAGAA GAGTCAGCTG ATGCCCCGAG CCCATTAGCT
    GAGCCCAGTC TCACTAAAGC TATCACACCT GAACCAGAGA GCTCTGAGCC AGAGAAATCC
    TCCTCACCAC CACCTCAAAG TCTGTCTGGA TCCCTTACCC AGCACGAAAA AGCCGTCAAT
    GGGCTCACGG ATGTTGACGC TGCTCCACTG AGTGAAGAAT TGGAAACCCA ACCCAGGGAA
    GCCTCTCCAT TGCTGCCTAC TTCCAGTGTG CCCCAGTCAG AGCCCAGGCC AGTCACACCG
    GTGCTGGAGG AAGAGTCTGA TCCAATCAAT ATGAACAGTC CTTTGCCTCC TGTTGAGGAC
    GATGCTGGAT GTCCTGACAA CGTTTCCCCC TCCCTCTCCA CCTCCACGAC TGCTGCTATC
    TCCACCACTC CTCCTGCTCC TCCTCCAGGA CTCTCGCACC CCAGTCAGGT TTCTGCAGCG
    TTGGACAGGA GACCCTCTAA TGGAGCAGAG ATCAAAGAAA CAGGGAAGGA AAACGAGGCC
    TTGCCGGACA AGAGAGGAGA GCCTTTCTTA CAGTCCAGAA AAAGCTCAAA TCAAGCTACA
    TCTAGTGCAC CAAAGACCTG GAAGAAGCCA AAAGAGGACA TGCCTGTAGG GCAAGATCAG
    TGTCCAGACA AAGAGGAGGA CACTGATCAG GCCAGTATGG AACCTGCTCC TGAAAGCCCC
    AGTCCTCCTC CTGTGGAGGT GGAGAGGAGG CCATCAGTAG GAGCAGTGCT CGAGGAGAAT
    GGAGAACAGG AAGTGGAGCC TTTGAGAAAC GGTGCTGAAC ACACTTCTGA AACTGAGAGC
    AGTGACAGCA TCCACCCGCC TGCAGTCAAA GAGCCGTTGA TCAAAGTATT GCCACAGCCT
    GCAGAGAAGA ATGGAAAGAA GCAGTACAAC AGAGACTTCC TGCTGGGCTT CCAGTTTATG
    CCTGCATGTG TGCAGAAACC AGAAGGGCTT CCTCCCATTT CTGATGTTGT TCTAGATAAG
    ATTAACCAAA ATAAGCTGCC CATGCGGCCG GTAGACCCAC GTGTGCTTTC CAGAGGCCCG
    GACTTTACGC CAGCGTTTGC GGATTTCAGC CGACCCATTT CAGGAGGCAG AGGGGCACCG
    TTGCTGAATG TTGGCATGCG ACGGCCACCT GGACGCAAGA TCATCACAAA CGTGTCTGTG
    ATTGATGAGG TGCAGCTGAA GACAACAGAG AATGCCTGGA AGCCTGGCAT GAAGCGGGAA
    GGTGCAGTTG AAGATCCAGA TGCCCAGAGA ACTCAGGAGC TTTTCCGAAA AGTGCGCAGT
    ATTCTCAATA AACTGACACC GCAGATGTTC AGCCAGCTGA TGAAGCAGGT GACTGACCTC
    ACCATCGACA CAGAGGAGCG ACTCAAGGGT GTCATAGATC TGGTCTTTGA AAAAGCAATC
    AATGAGCCCA GCTTCTCTGT GGCCTACGGC AACATGTGCA GCTGTCTGGC CACGTTAAAA
    GTGCCCATGA CGGATAAGCC CAACAGCACT GTGAATTTCC GGAAGCTGCT TCTGAACCGC
    TGTCAGAAAG AGTTTGAGAA GGACAAGATG GATGACGATG CCTTTGAGAA GAAACACAGG
    GAGCTGGAGG CAGCTACTGC GTCCAGTGAG CGGGAGAGGC TTCAGGAGGA GCTGGAAGAG
    GCCAAAGACA AGGCCCGAAG ACGCTCCATT GGCAACATTA AGTTCATCGG CGAGCTCTTC
    AAGCTGCGGA TGCTGACAGA GGCCATCATG CATGACTGCG TGGTGAAGTT GCTGAAAAAC
    CATGATGAGG AATCACTGGA GTGCCTGTGT AGACTGCTCA CCACTATTGG AAAGGACCTG
    GACTTTGAGA AAGCTAAGCC ACGAATGGAT CAGTACTTTA ATCAGATGGA GAAGATAGTT
    AAGGAGAGGA AGACTTCTTC ACGCATTCGC TTCATGCTGC AGGATGTTAT TGATCTGCGA
    TTGCACAACT GGGTTTCTCG AAGAGCAGAT CAGGGCCCCA AGACCATCGA GCAGATCCAT
    AAAGACGCAA AGCTGGAGGA GCAGGAGGAA CAGAGGAAAG TCCACCAGCA ACTGCTTTCC
    AAAGACAACA AGAGGAGACC AGTGGTGCAG AGAGAGGAGA CCTGGAGCAC TGTGCCTATG
    ACTAAGAACA GCAGAACAAT AGACCCCGCC AAGATTCCCA AATTCTCCAA GCCAGCGATA
    GATGAGAAGA TTCAGCTGGG GCCGAGAGCT CAGGTGAACT GGATGAAGGG CAGCAGTGGT
    GGAGCTGGAG CAAAGGCCAG TGAGTCAGAT GCGTCTCGGC CGAGTGCCAG TTTGAATCGT
    TACTCTCCTC TGCAACCCTC GGCACTCCAG ACTTCTTCTT TACCCTCCAC ATCTCCAGAT
    TTTGATTCCA GGAGAGTTCT CGGCAGTCGT GGAAGCTCAG GACGGGAGCG CAATGACAAG
    CCATTGAGTG CGGGTCCTGC TCGGACAGGG CCTATTTCAT TGAGCAGTAG CAATAAGGAG
    ACCCCCGAAG AGCTGGTTCA GGAAGTGTCC CGCAGAGACT CCAATGCTTC AGATACACCC
    AAGCTGCTCG TCAGTACTGC AGACAAGAGC AGACTGGAGA ACAGCCAACC CAGAGAGTCT
    GCTGTTAAAC TTGAAGCACT CTCAGGGCCC TCTCCAGATA AACCTGCATT ATCAGAAGAG
    GAGATGGAGA GGCGGTCCAA GTCTATAATT GATGAGTTTT TGCACATTAA TGATTACAAG
    GAAGCTTTGC AGTGTGTTGA GGAACTAGAG CAGTCTGCCA TGCTCTATGT GTTTGTGCGG
    GTGGGTGTGG AGTCCACGCT GGAAAGGAGT CAGATCACGC GAGACCATAT GGGTCAGCTG
    CTCTTCCAGC TTCTGCAGGC TGGAGTCCTC CCTAAACTTC AGTTCTTTAA AGGGTTTTCT
    GAAACTCTTG AATTAGCAGA TGATATGGCC ATTGATATTC CCCATATATG GCTGTACCTT
    GCGGAGCTGG TAACCCCTGT GCTTCGAGAG GGGGGAATCT CTATGAGAGA ATTATTTAGT
    GAATTTAGCA AACCATTACT CCCTGTGGGA AGAGCTGGGA TATTATTTTC TGAAATATTG
    CACCTTCTAT GCAAACAAAT GAGCCATAAG AAAGTGGGAT CATTATGGAG GGATTCTGGG
    TTGAGCTGGG CAGATTTTAT ACCTGAAACA GAGGATGTGC ACAGCTTCAT CACAGAACAT
    AAACTAGAGT TCACTCTGTC TGATGGCTCA AACCCGACCG AGGCTGTCTC TAAGAGAACA
    CTCTCGCCTG AGGAACTGAA CAAGCAACTT GAAAAACTTT TACTTGAGGA CATGGTTGGC
    GATGAACAAA TCTTCGACTG GGTTGAGGCC AATCTAGATG AATCAGAAAT GAGTTCAGCT
    CCTTTCGTCA GAGCTTTAAT GACTGCTGTG TGTAAAGCAG CAGTAAAAAC TGAGGGCTCC
    TCGTGTAAAG TGGACTTGTC CATAATTCAG ACGAGATTGC CTGTATTACA CAAGTACCTT
    AACTCAGACA CAGAGAGACA GTTGCAAGCA CTTTATGCAC TTCAAGCATT GATAGTAAAA
    CTGGACCAGC CTGCTAATTT GCTCCGGATG TTTTTTGACT GTTTGTATGA TGAGGACGTG
    ATCTCTGAAG ACGCTTTCTA TAAATGGGAG GTCAGTAAAG ACCCCGCTGA ACAGCAAGGC
    AAAGGTGTTG CCCTGAAGTC CGTCACAGCC TTCTTCACGT GGCTGCGCGA GGCCGAGGAA
    GAGTCCGAGG ACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa151560
    Clone ID Related Accession (Same CDS sequence) XM_021469957.1
    Accession Version XM_021469957.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACTAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CAACAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTTTCCAG AGGACGCAGA TGCAAACGGC CCGGCCCACC
    ATTCCAAGCA ACACCCCACC CATTCGGCCC ACGTCTCAGA CCCCCACGGC AGCAGTATAT
    TCTCCCAATC AGCACATCAT GATGACCATG GCTCACATGC CCTTTCATTC CCCACAGACA
    GCGCAGTACT ACATCCCTCA GTATCGTCAC AGTGCTCCAC AGTATGTGGG ACCTCCTCAA
    CAGTACCCTG TCCAGCCGAC TGGACCCAGT ACCTTCTATG CTGCAGCCAG TCCAGGGGAG
    TTTCCTGCAC CTTATGCAGC AGGACCTCCC TACTATCCTG GGCAGCCCGT GTACACACCA
    TCTCCACCCA TTATAGTGCC TACACCACAA CAACCTCCAC CTGCTAAGAG GGAAAAGAAA
    ACAATCCGCA TCCGAGACCC TAACCAGGGT GGCAAGGATG TCACAGACGA AATATTATCA
    GGAGTGGGTT TGAGTCGAAA CCCAACCCCG CCTGTGGGAC GGCCCTCTTC CACTCCTACA
    CCTCCACAGT TTTTATGTCC ACACCCTCAT TATCCTCACA TTTTCTACCT CAAATCCCAG
    CAGCTGAACA GCCAGGTAGC AGATCATGGG CACATCATGT ATAATGTGGA CAGCAGCCCG
    CACCTTCCAG CATCCTTCAA CTTGAAAGCA GACGATAAGC CAAAACTAGA GTTTTCCTTG
    CAAAGAACTG CATCTCCTGG TCTGCGGCAG CCCGACACTC CTCTGGAGAG AAGAGATCCC
    AGCAGCCCAG TCCAAACACC CTCCTCTCCC CCACACAAAC CTGAGCTTCC CCCCTCCGAC
    TCAGAGACGG CCTCAAGTGT AGCTACAGCC CCGACTCCTT CCATACCTGC CTCAACAGAA
    GAGTCAGCTG ATGCCCCGAG CCCATTAGCT GAGCCCAGTC TCACTAAAGC TATCACACCT
    GAACCAGAGA GCTCTGAGCC AGAGAAATCC TCCTCACCAC CACCTCAAAG TCTGTCTGGA
    TCCCTTACCC AGCACGAAAA AGCCGTCAAT GGGCTCACGG ATGTTGACGC TGCTCCACTG
    AGTGAAGAAT TGGAAACCCA ACCCAGGGAA GCCTCTCCAT TGCTGCCTAC TTCCAGTGTG
    CCCCAGTCAG AGCCCAGGCC AGTCACACCG GTGCTGGAGG AAGAGTCTGA TCCAATCAAT
    ATGAACAGTC CTTTGCCTCC TGTTGAGGAC GATGCTGGAT GTCCTGACAA CGTTTCCCCC
    TCCCTCTCCA CCTCCACGAC TGCTGCTATC TCCACCACTC CTCCTGCTCC TCCTCCAGGA
    CTCTCGCACC CCAGTCAGGT TTCTGCAGCG TTGGACAGGA GACCCTCTAA TGGAGCAGAG
    ATCAAAGAAA CAGGGAAGGA AAACGAGGCC TTGCCGGACA AGAGAGGAGA GCCTTTCTTA
    CAGTCCAGAA AAAGCTCAAA TCAAGCTACA TCTAGTGCAC CAAAGACCTG GAAGAAGCCA
    AAAGAGGACA TGCCTGTAGG GCAAGATCAG TGTCCAGACA AAGAGGAGGA CACTGATCAG
    GCCAGTATGG AACCTGCTCC TGAAAGCCCC AGTCCTCCTC CTGTGGAGGT GGAGAGGAGG
    CCATCAGTAG GAGCAGTGCT CGAGGAGAAT GGAGAACAGG AAGTGGAGCC TTTGAGAAAC
    GGTGCTGAAC ACACTTCTGA AACTGAGAGC AGTGACAGCA TCCACCCGCC TGCAGTCAAA
    GAGCCGTTGA TCAAAGTATT GCCACAGCCT GCAGAGAAGA ATGGAAAGAA GCAGTACAAC
    AGAGACTTCC TGCTGGGCTT CCAGTTTATG CCTGCATGTG TGCAGAAACC AGAAGGGCTT
    CCTCCCATTT CTGATGTTGT TCTAGATAAG ATTAACCAAA ATAAGCTGCC CATGCGGCCG
    GTAGACCCAC GTGTGCTTTC CAGAGGCCCG GACTTTACGC CAGCGTTTGC GGATTTCAGC
    CGACCCATTT CAGGAGGCAG AGGGGCACCG TTGCTGAATG TTGGCATGCG ACGGCCACCT
    GGACGCAAGA TCATCACAAA CGTGTCTGTG ATTGATGAGG TGCAGCTGAA GACAACAGAG
    AATGCCTGGA AGCCTGGCAT GAAGCGGGAA GGTGCAGTTG AAGATCCAGA TGCCCAGAGA
    ACTCAGGAGC TTTTCCGAAA AGTGCGCAGT ATTCTCAATA AACTGACACC GCAGATGTTC
    AGCCAGCTGA TGAAGCAGGT GACTGACCTC ACCATCGACA CAGAGGAGCG ACTCAAGGGT
    GTCATAGATC TGGTCTTTGA AAAAGCAATC AATGAGCCCA GCTTCTCTGT GGCCTACGGC
    AACATGTGCA GCTGTCTGGC CACGTTAAAA GTGCCCATGA CGGATAAGCC CAACAGCACT
    GTGAATTTCC GGAAGCTGCT TCTGAACCGC TGTCAGAAAG AGTTTGAGAA GGACAAGATG
    GATGACGATG CCTTTGAGAA GAAACACAGG GAGCTGGAGG CAGCTACTGC GTCCAGTGAG
    CGGGAGAGGC TTCAGGAGGA GCTGGAAGAG GCCAAAGACA AGGCCCGAAG ACGCTCCATT
    GGCAACATTA AGTTCATCGG CGAGCTCTTC AAGCTGCGGA TGCTGACAGA GGCCATCATG
    CATGACTGCG TGGTGAAGTT GCTGAAAAAC CATGATGAGG AATCACTGGA GTGCCTGTGT
    AGACTGCTCA CCACTATTGG AAAGGACCTG GACTTTGAGA AAGCTAAGCC ACGAATGGAT
    CAGTACTTTA ATCAGATGGA GAAGATAGTT AAGGAGAGGA AGACTTCTTC ACGCATTCGC
    TTCATGCTGC AGGATGTTAT TGATCTGCGA TTGCACAACT GGGTTTCTCG AAGAGCAGAT
    CAGGGCCCCA AGACCATCGA GCAGATCCAT AAAGACGCAA AGCTGGAGGA GCAGGAGGAA
    CAGAGGAAAG TCCACCAGCA ACTGCTTTCC AAAGACAACA AGAGGAGACC AGTGGTGCAG
    AGAGAGGAGA CCTGGAGCAC TGTGCCTATG ACTAAGAACA GCAGAACAAT AGACCCCGCC
    AAGATTCCCA AATTCTCCAA GCCAGCGATA GATGAGAAGA TTCAGCTGGG GCCGAGAGCT
    CAGGTGAACT GGATGAAGGG CAGCAGTGGT GGAGCTGGAG CAAAGGCCAG TGAGTCAGAT
    GCGTCTCGGC CGAGTGCCAG TTTGAATCGT TACTCTCCTC TGCAACCCTC GGCACTCCAG
    ACTTCTTCTT TACCCTCCAC ATCTCCAGAT TTTGATTCCA GGAGAGTTCT CGGCAGTCGT
    GGAAGCTCAG GACGGGAGCG CAATGACAAG CCATTGAGTG CGGGTCCTGC TCGGACAGGG
    CCTATTTCAT TGAGCAGTAG CAATAAGGAG ACCCCCGAAG AGCTGGTTCA GGAAGTGTCC
    CGCAGAGACT CCAATGCTTC AGATACACCC AAGCTGCTCG TCAGTACTGC AGACAAGAGC
    AGACTGGAGA ACAGCCAACC CAGAGAGTCT GCTGTTAAAC TTGAAGCACT CTCAGGGCCC
    TCTCCAGATA AACCTGCATT ATCAGAAGAG GAGATGGAGA GGCGGTCCAA GTCTATAATT
    GATGAGTTTT TGCACATTAA TGATTACAAG GAAGCTTTGC AGTGTGTTGA GGAACTAGAG
    CAGTCTGCCA TGCTCTATGT GTTTGTGCGG GTGGGTGTGG AGTCCACGCT GGAAAGGAGT
    CAGATCACGC GAGACCATAT GGGTCAGCTG CTCTTCCAGC TTCTGCAGGC TGGAGTCCTC
    CCTAAACTTC AGTTCTTTAA AGGGTTTTCT GAAACTCTTG AATTAGCAGA TGATATGGCC
    ATTGATATTC CCCATATATG GCTGTACCTT GCGGAGCTGG TAACCCCTGT GCTTCGAGAG
    GGGGGAATCT CTATGAGAGA ATTATTTAGT GAATTTAGCA AACCATTACT CCCTGTGGGA
    AGAGCTGGGA TATTATTTTC TGAAATATTG CACCTTCTAT GCAAACAAAT GAGCCATAAG
    AAAGTGGGAT CATTATGGAG GGATTCTGGG TTGAGCTGGG CAGATTTTAT ACCTGAAACA
    GAGGATGTGC ACAGCTTCAT CACAGAACAT AAACTAGAGT TCACTCTGTC TGATGGCTCA
    AACCCGACCG AGGCTGTCTC TAAGAGAACA CTCTCGCCTG AGGAACTGAA CAAGCAACTT
    GAAAAACTTT TACTTGAGGA CATGGTTGGC GATGAACAAA TCTTCGACTG GGTTGAGGCC
    AATCTAGATG AATCAGAAAT GAGTTCAGCT CCTTTCGTCA GAGCTTTAAT GACTGCTGTG
    TGTAAAGCAG CAGTAAAAAC TGAGGGCTCC TCGTGTAAAG TGGACTTGTC CATAATTCAG
    ACGAGATTGC CTGTATTACA CAAGTACCTT AACTCAGACA CAGAGAGACA GTTGCAAGCA
    CTTTATGCAC TTCAAGCATT GATAGTAAAA CTGGACCAGC CTGCTAATTT GCTCCGGATG
    TTTTTTGACT GTTTGTATGA TGAGGACGTG ATCTCTGAAG ACGCTTTCTA TAAATGGGAG
    GTCAGTAAAG ACCCCGCTGA ACAGCAAGGC AAAGGTGTTG CCCTGAAGTC CGTCACAGCC
    TTCTTCACGT GGCTGCGCGA GGCCGAGGAA GAGTCCGAGG ACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325632.1
    CDS458..5605
    Translation

    Target ORF information:

    RefSeq Version XM_021469957.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469957.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACTAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CAACAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTTTCCAG AGGACGCAGA TGCAAACGGC CCGGCCCACC
    ATTCCAAGCA ACACCCCACC CATTCGGCCC ACGTCTCAGA CCCCCACGGC AGCAGTATAT
    TCTCCCAATC AGCACATCAT GATGACCATG GCTCACATGC CCTTTCATTC CCCACAGACA
    GCGCAGTACT ACATCCCTCA GTATCGTCAC AGTGCTCCAC AGTATGTGGG ACCTCCTCAA
    CAGTACCCTG TCCAGCCGAC TGGACCCAGT ACCTTCTATG CTGCAGCCAG TCCAGGGGAG
    TTTCCTGCAC CTTATGCAGC AGGACCTCCC TACTATCCTG GGCAGCCCGT GTACACACCA
    TCTCCACCCA TTATAGTGCC TACACCACAA CAACCTCCAC CTGCTAAGAG GGAAAAGAAA
    ACAATCCGCA TCCGAGACCC TAACCAGGGT GGCAAGGATG TCACAGACGA AATATTATCA
    GGAGTGGGTT TGAGTCGAAA CCCAACCCCG CCTGTGGGAC GGCCCTCTTC CACTCCTACA
    CCTCCACAGT TTTTATGTCC ACACCCTCAT TATCCTCACA TTTTCTACCT CAAATCCCAG
    CAGCTGAACA GCCAGGTAGC AGATCATGGG CACATCATGT ATAATGTGGA CAGCAGCCCG
    CACCTTCCAG CATCCTTCAA CTTGAAAGCA GACGATAAGC CAAAACTAGA GTTTTCCTTG
    CAAAGAACTG CATCTCCTGG TCTGCGGCAG CCCGACACTC CTCTGGAGAG AAGAGATCCC
    AGCAGCCCAG TCCAAACACC CTCCTCTCCC CCACACAAAC CTGAGCTTCC CCCCTCCGAC
    TCAGAGACGG CCTCAAGTGT AGCTACAGCC CCGACTCCTT CCATACCTGC CTCAACAGAA
    GAGTCAGCTG ATGCCCCGAG CCCATTAGCT GAGCCCAGTC TCACTAAAGC TATCACACCT
    GAACCAGAGA GCTCTGAGCC AGAGAAATCC TCCTCACCAC CACCTCAAAG TCTGTCTGGA
    TCCCTTACCC AGCACGAAAA AGCCGTCAAT GGGCTCACGG ATGTTGACGC TGCTCCACTG
    AGTGAAGAAT TGGAAACCCA ACCCAGGGAA GCCTCTCCAT TGCTGCCTAC TTCCAGTGTG
    CCCCAGTCAG AGCCCAGGCC AGTCACACCG GTGCTGGAGG AAGAGTCTGA TCCAATCAAT
    ATGAACAGTC CTTTGCCTCC TGTTGAGGAC GATGCTGGAT GTCCTGACAA CGTTTCCCCC
    TCCCTCTCCA CCTCCACGAC TGCTGCTATC TCCACCACTC CTCCTGCTCC TCCTCCAGGA
    CTCTCGCACC CCAGTCAGGT TTCTGCAGCG TTGGACAGGA GACCCTCTAA TGGAGCAGAG
    ATCAAAGAAA CAGGGAAGGA AAACGAGGCC TTGCCGGACA AGAGAGGAGA GCCTTTCTTA
    CAGTCCAGAA AAAGCTCAAA TCAAGCTACA TCTAGTGCAC CAAAGACCTG GAAGAAGCCA
    AAAGAGGACA TGCCTGTAGG GCAAGATCAG TGTCCAGACA AAGAGGAGGA CACTGATCAG
    GCCAGTATGG AACCTGCTCC TGAAAGCCCC AGTCCTCCTC CTGTGGAGGT GGAGAGGAGG
    CCATCAGTAG GAGCAGTGCT CGAGGAGAAT GGAGAACAGG AAGTGGAGCC TTTGAGAAAC
    GGTGCTGAAC ACACTTCTGA AACTGAGAGC AGTGACAGCA TCCACCCGCC TGCAGTCAAA
    GAGCCGTTGA TCAAAGTATT GCCACAGCCT GCAGAGAAGA ATGGAAAGAA GCAGTACAAC
    AGAGACTTCC TGCTGGGCTT CCAGTTTATG CCTGCATGTG TGCAGAAACC AGAAGGGCTT
    CCTCCCATTT CTGATGTTGT TCTAGATAAG ATTAACCAAA ATAAGCTGCC CATGCGGCCG
    GTAGACCCAC GTGTGCTTTC CAGAGGCCCG GACTTTACGC CAGCGTTTGC GGATTTCAGC
    CGACCCATTT CAGGAGGCAG AGGGGCACCG TTGCTGAATG TTGGCATGCG ACGGCCACCT
    GGACGCAAGA TCATCACAAA CGTGTCTGTG ATTGATGAGG TGCAGCTGAA GACAACAGAG
    AATGCCTGGA AGCCTGGCAT GAAGCGGGAA GGTGCAGTTG AAGATCCAGA TGCCCAGAGA
    ACTCAGGAGC TTTTCCGAAA AGTGCGCAGT ATTCTCAATA AACTGACACC GCAGATGTTC
    AGCCAGCTGA TGAAGCAGGT GACTGACCTC ACCATCGACA CAGAGGAGCG ACTCAAGGGT
    GTCATAGATC TGGTCTTTGA AAAAGCAATC AATGAGCCCA GCTTCTCTGT GGCCTACGGC
    AACATGTGCA GCTGTCTGGC CACGTTAAAA GTGCCCATGA CGGATAAGCC CAACAGCACT
    GTGAATTTCC GGAAGCTGCT TCTGAACCGC TGTCAGAAAG AGTTTGAGAA GGACAAGATG
    GATGACGATG CCTTTGAGAA GAAACACAGG GAGCTGGAGG CAGCTACTGC GTCCAGTGAG
    CGGGAGAGGC TTCAGGAGGA GCTGGAAGAG GCCAAAGACA AGGCCCGAAG ACGCTCCATT
    GGCAACATTA AGTTCATCGG CGAGCTCTTC AAGCTGCGGA TGCTGACAGA GGCCATCATG
    CATGACTGCG TGGTGAAGTT GCTGAAAAAC CATGATGAGG AATCACTGGA GTGCCTGTGT
    AGACTGCTCA CCACTATTGG AAAGGACCTG GACTTTGAGA AAGCTAAGCC ACGAATGGAT
    CAGTACTTTA ATCAGATGGA GAAGATAGTT AAGGAGAGGA AGACTTCTTC ACGCATTCGC
    TTCATGCTGC AGGATGTTAT TGATCTGCGA TTGCACAACT GGGTTTCTCG AAGAGCAGAT
    CAGGGCCCCA AGACCATCGA GCAGATCCAT AAAGACGCAA AGCTGGAGGA GCAGGAGGAA
    CAGAGGAAAG TCCACCAGCA ACTGCTTTCC AAAGACAACA AGAGGAGACC AGTGGTGCAG
    AGAGAGGAGA CCTGGAGCAC TGTGCCTATG ACTAAGAACA GCAGAACAAT AGACCCCGCC
    AAGATTCCCA AATTCTCCAA GCCAGCGATA GATGAGAAGA TTCAGCTGGG GCCGAGAGCT
    CAGGTGAACT GGATGAAGGG CAGCAGTGGT GGAGCTGGAG CAAAGGCCAG TGAGTCAGAT
    GCGTCTCGGC CGAGTGCCAG TTTGAATCGT TACTCTCCTC TGCAACCCTC GGCACTCCAG
    ACTTCTTCTT TACCCTCCAC ATCTCCAGAT TTTGATTCCA GGAGAGTTCT CGGCAGTCGT
    GGAAGCTCAG GACGGGAGCG CAATGACAAG CCATTGAGTG CGGGTCCTGC TCGGACAGGG
    CCTATTTCAT TGAGCAGTAG CAATAAGGAG ACCCCCGAAG AGCTGGTTCA GGAAGTGTCC
    CGCAGAGACT CCAATGCTTC AGATACACCC AAGCTGCTCG TCAGTACTGC AGACAAGAGC
    AGACTGGAGA ACAGCCAACC CAGAGAGTCT GCTGTTAAAC TTGAAGCACT CTCAGGGCCC
    TCTCCAGATA AACCTGCATT ATCAGAAGAG GAGATGGAGA GGCGGTCCAA GTCTATAATT
    GATGAGTTTT TGCACATTAA TGATTACAAG GAAGCTTTGC AGTGTGTTGA GGAACTAGAG
    CAGTCTGCCA TGCTCTATGT GTTTGTGCGG GTGGGTGTGG AGTCCACGCT GGAAAGGAGT
    CAGATCACGC GAGACCATAT GGGTCAGCTG CTCTTCCAGC TTCTGCAGGC TGGAGTCCTC
    CCTAAACTTC AGTTCTTTAA AGGGTTTTCT GAAACTCTTG AATTAGCAGA TGATATGGCC
    ATTGATATTC CCCATATATG GCTGTACCTT GCGGAGCTGG TAACCCCTGT GCTTCGAGAG
    GGGGGAATCT CTATGAGAGA ATTATTTAGT GAATTTAGCA AACCATTACT CCCTGTGGGA
    AGAGCTGGGA TATTATTTTC TGAAATATTG CACCTTCTAT GCAAACAAAT GAGCCATAAG
    AAAGTGGGAT CATTATGGAG GGATTCTGGG TTGAGCTGGG CAGATTTTAT ACCTGAAACA
    GAGGATGTGC ACAGCTTCAT CACAGAACAT AAACTAGAGT TCACTCTGTC TGATGGCTCA
    AACCCGACCG AGGCTGTCTC TAAGAGAACA CTCTCGCCTG AGGAACTGAA CAAGCAACTT
    GAAAAACTTT TACTTGAGGA CATGGTTGGC GATGAACAAA TCTTCGACTG GGTTGAGGCC
    AATCTAGATG AATCAGAAAT GAGTTCAGCT CCTTTCGTCA GAGCTTTAAT GACTGCTGTG
    TGTAAAGCAG CAGTAAAAAC TGAGGGCTCC TCGTGTAAAG TGGACTTGTC CATAATTCAG
    ACGAGATTGC CTGTATTACA CAAGTACCTT AACTCAGACA CAGAGAGACA GTTGCAAGCA
    CTTTATGCAC TTCAAGCATT GATAGTAAAA CTGGACCAGC CTGCTAATTT GCTCCGGATG
    TTTTTTGACT GTTTGTATGA TGAGGACGTG ATCTCTGAAG ACGCTTTCTA TAAATGGGAG
    GTCAGTAAAG ACCCCGCTGA ACAGCAAGGC AAAGGTGTTG CCCTGAAGTC CGTCACAGCC
    TTCTTCACGT GGCTGCGCGA GGCCGAGGAA GAGTCCGAGG ACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27748
    Clone ID Related Accession (Same CDS sequence) XM_001920046.5
    Accession Version XM_001920046.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5130bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001920046.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACTAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CAACAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTGCCCCT GGGCCTCGTC CTACCCATCA CCAGTTTTTC
    CAGAGGACGC AGATGCAAAC GGCCCGGCCC ACCATTCCAA GCAACACCCC ACCCATTCGG
    CCCACGTCTC AGACCCCCAC GGCAGCAGTA TATTCTCCCA ATCAGCACAT CATGATGACC
    ATGGCTCACA TGCCCTTTCA TTCCCCACAG ACAGCGCAGT ACTACATCCC TCAGTATCGT
    CACAGTGCTC CACAGTATGT GGGACCTCCT CAACAGTACC CTGTCCAGCC GACTGGACCC
    AGTACCTTCT ATGCTGCAGC CAGTCCAGGG GAGTTTCCTG CACCTTATGC AGCAGGACCT
    CCCTACTATC CTGGGCAGCC CGTGTACACA CCATCTCCAC CCATTATAGT GCCTACACCA
    CAACAACCTC CACCTGCTAA GAGGGAAAAG AAAACAATCC GCATCCGAGA CCCTAACCAG
    GGTGGCAAGG ATGTCACAGA CGAAATATTA TCAGGAGTGG GTTTGAGTCG AAACCCAACC
    CCGCCTGTGG GACGGCCCTC TTCCACTCCT ACACCTCCAC AGCAGCTGAA CAGCCAGGTA
    GCAGATCATG GGCACATCAT GTATAATGTG GACAGCAGCC CGCACCTTCC AGCATCCTTC
    AACTTGAAAG CAGACGATAA GCCAAAACTA GAGTTTTCCT TGCAAAGAAC TGCATCTCCT
    GGTCTGCGGC AGCCCGACAC TCCTCTGGAG AGAAGAGATC CCAGCAGCCC AGTCCAAACA
    CCCTCCTCTC CCCCACACAA ACCTGAGCTT CCCCCCTCCG ACTCAGAGAC GGCCTCAAGT
    GTAGCTACAG CCCCGACTCC TTCCATACCT GCCTCAACAG AAGAGTCAGC TGATGCCCCG
    AGCCCATTAG CTGAGCCCAG TCTCACTAAA GCTATCACAC CTGAACCAGA GAGCTCTGAG
    CCAGAGAAAT CCTCCTCACC ACCACCTCAA AGTCTGTCTG GATCCCTTAC CCAGCACGAA
    AAAGCCGTCA ATGGGCTCAC GGATGTTGAC GCTGCTCCAC TGAGTGAAGA ATTGGAAACC
    CAACCCAGGG AAGCCTCTCC ATTGCTGCCT ACTTCCAGTG TGCCCCAGTC AGAGCCCAGG
    CCAGTCACAC CGGTGCTGGA GGAAGAGTCT GATCCAATCA ATATGAACAG TCCTTTGCCT
    CCTGTTGAGG ACGATGCTGG ATGTCCTGAC AACGTTTCCC CCTCCCTCTC CACCTCCACG
    ACTGCTGCTA TCTCCACCAC TCCTCCTGCT CCTCCTCCAG GACTCTCGCA CCCCAGTCAG
    GTTTCTGCAG CGTTGGACAG GAGACCCTCT AATGGAGCAG AGATCAAAGA AACAGGGAAG
    GAAAACGAGG CCTTGCCGGA CAAGAGAGGA GAGCCTTTCT TACAGTCCAG AAAAAGCTCA
    AATCAAGCTA CATCTAGTGC ACCAAAGACC TGGAAGAAGC CAAAAGAGGA CATGCCTGTA
    GGGCAAGATC AGTGTCCAGA CAAAGAGGAG GACACTGATC AGGCCAGTAT GGAACCTGCT
    CCTGAAAGCC CCAGTCCTCC TCCTGTGGAG GTGGAGAGGA GGCCATCAGT AGGAGCAGTG
    CTCGAGGAGA ATGGAGAACA GGAAGTGGAG CCTTTGAGAA ACGGTGCTGA ACACACTTCT
    GAAACTGAGA GCAGTGACAG CATCCACCCG CCTGCAGTCA AAGAGCCGTT GATCAAAGTA
    TTGCCACAGC CTGCAGAGAA GAATGGAAAG AAGCAGTACA ACAGAGACTT CCTGCTGGGC
    TTCCAGTTTA TGCCTGCATG TGTGCAGAAA CCAGAAGGGC TTCCTCCCAT TTCTGATGTT
    GTTCTAGATA AGATTAACCA AAATAAGCTG CCCATGCGGC CGGTAGACCC ACGTGTGCTT
    TCCAGAGGCC CGGACTTTAC GCCAGCGTTT GCGGATTTCA GCCGACCCAT TTCAGGAGGC
    AGAGGGGCAC CGTTGCTGAA TGTTGGCATG CGACGGCCAC CTGGACGCAA GATCATCACA
    AACGTGTCTG TGATTGATGA GGTGCAGCTG AAGACAACAG AGAATGCCTG GAAGCCTGGC
    ATGAAGCGGG AAGGTGCAGT TGAAGATCCA GATGCCCAGA GAACTCAGGA GCTTTTCCGA
    AAAGTGCGCA GTATTCTCAA TAAACTGACA CCGCAGATGT TCAGCCAGCT GATGAAGCAG
    GTGACTGACC TCACCATCGA CACAGAGGAG CGACTCAAGG GTGTCATAGA TCTGGTCTTT
    GAAAAAGCAA TCAATGAGCC CAGCTTCTCT GTGGCCTACG GCAACATGTG CAGCTGTCTG
    GCCACGTTAA AAGTGCCCAT GACGGATAAG CCCAACAGCA CTGTGAATTT CCGGAAGCTG
    CTTCTGAACC GCTGTCAGAA AGAGTTTGAG AAGGACAAGA TGGATGACGA TGCCTTTGAG
    AAGAAACACA GGGAGCTGGA GGCAGCTACT GCGTCCAGTG AGCGGGAGAG GCTTCAGGAG
    GAGCTGGAAG AGGCCAAAGA CAAGGCCCGA AGACGCTCCA TTGGCAACAT TAAGTTCATC
    GGCGAGCTCT TCAAGCTGCG GATGCTGACA GAGGCCATCA TGCATGACTG CGTGGTGAAG
    TTGCTGAAAA ACCATGATGA GGAATCACTG GAGTGCCTGT GTAGACTGCT CACCACTATT
    GGAAAGGACC TGGACTTTGA GAAAGCTAAG CCACGAATGG ATCAGTACTT TAATCAGATG
    GAGAAGATAG TTAAGGAGAG GAAGACTTCT TCACGCATTC GCTTCATGCT GCAGGATGTT
    ATTGATCTGC GATTGCACAA CTGGGTTTCT CGAAGAGCAG ATCAGGGCCC CAAGACCATC
    GAGCAGATCC ATAAAGACGC AAAGCTGGAG GAGCAGGAGG AACAGAGGAA AGTCCACCAG
    CAACTGCTTT CCAAAGACAA CAAGAGGAGA CCAGTGGTGC AGAGAGAGGA GACCTGGAGC
    ACTGTGCCTA TGACTAAGAA CAGCAGAACA ATAGACCCCG CCAAGATTCC CAAATTCTCC
    AAGCCAGCGA TAGATGAGAA GATTCAGCTG GGGCCGAGAG CTCAGGTGAA CTGGATGAAG
    GGCAGCAGTG GTGGAGCTGG AGCAAAGGCC AGTGAGTCAG ATGCGTCTCG GCCGAGTGCC
    AGTTTGAATC GTTACTCTCC TCTGCAACCC TCGGCACTCC AGACTTCTTC TTTACCCTCC
    ACATCTCCAG ATTTTGATTC CAGGAGAGTT CTCGGCAGTC GTGGAAGCTC AGGACGGGAG
    CGCAATGACA AGCCATTGAG TGCGGGTCCT GCTCGGACAG GGCCTATTTC ATTGAGCAGT
    AGCAATAAGG AGACCCCCGA AGAGCTGGTT CAGGAAGTGT CCCGCAGAGA CTCCAATGCT
    TCAGATACAC CCAAGCTGCT CGTCAGTACT GCAGACAAGA GCAGACTGGA GAACAGCCAA
    CCCAGAGAGT CTGCTGTTAA ACTTGAAGCA CTCTCAGGGC CCTCTCCAGA TAAACCTGCA
    TTATCAGAAG AGGAGATGGA GAGGCGGTCC AAGTCTATAA TTGATGAGTT TTTGCACATT
    AATGATTACA AGGAAGCTTT GCAGTGTGTT GAGGAACTAG AGCAGTCTGC CATGCTCTAT
    GTGTTTGTGC GGGTGGGTGT GGAGTCCACG CTGGAAAGGA GTCAGATCAC GCGAGACCAT
    ATGGGTCAGC TGCTCTTCCA GCTTCTGCAG GCTGGAGTCC TCCCTAAACT TCAGTTCTTT
    AAAGGGTTTT CTGAAACTCT TGAATTAGCA GATGATATGG CCATTGATAT TCCCCATATA
    TGGCTGTACC TTGCGGAGCT GGTAACCCCT GTGCTTCGAG AGGGGGGAAT CTCTATGAGA
    GAATTATTTA GTGAATTTAG CAAACCATTA CTCCCTGTGG GAAGAGCTGG GATATTATTT
    TCTGAAATAT TGCACCTTCT ATGCAAACAA ATGAGCCATA AGAAAGTGGG ATCATTATGG
    AGGGATTCTG GGTTGAGCTG GGCAGATTTT ATACCTGAAA CAGAGGATGT GCACAGCTTC
    ATCACAGAAC ATAAACTAGA GTTCACTCTG TCTGATGGCT CAAACCCGAC CGAGGCTGTC
    TCTAAGAGAA CACTCTCGCC TGAGGAACTG AACAAGCAAC TTGAAAAACT TTTACTTGAG
    GACATGGTTG GCGATGAACA AATCTTCGAC TGGGTTGAGG CCAATCTAGA TGAATCAGAA
    ATGAGTTCAG CTCCTTTCGT CAGAGCTTTA ATGACTGCTG TGTGTAAAGC AGCAGTAAAA
    ACTGAGGGCT CCTCGTGTAA AGTGGACTTG TCCATAATTC AGACGAGATT GCCTGTATTA
    CACAAGTACC TTAACTCAGA CACAGAGAGA CAGTTGCAAG CACTTTATGC ACTTCAAGCA
    TTGATAGTAA AACTGGACCA GCCTGCTAAT TTGCTCCGGA TGTTTTTTGA CTGTTTGTAT
    GATGAGGACG TGATCTCTGA AGACGCTTTC TATAAATGGG AGGTCAGTAA AGACCCCGCT
    GAACAGCAAG GCAAAGGTGT TGCCCTGAAG TCCGTCACAG CCTTCTTCAC GTGGCTGCGC
    GAGGCCGAGG AAGAGTCCGA GGACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001920081.2
    CDS605..5734
    Translation

    Target ORF information:

    RefSeq Version XM_001920046.5
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio eukaryotic translation initiation factor 4 gamma, 3b (eif4g3b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001920046.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTTTGC CGCCGAAAGC GGTGCCGAAA CCAGCCGCTG CCGCCGCTGC TGGAACTGGA 
    CCTGGAACCG GGCAGTTATC CGCGAGCCAA CTGCGGACCA CCCTCACTAC AGTCCCGCTA
    CAGTCGGTGG CTCAACAAGT TTTCCTTAAT TTCCCCTCAA GATATCCACG AGTGCCGCCT
    CCCCTGGACG AGCGAATCTT CTCAACACAG CCTGTCTCTG CTGTCTACAG TGTACAGAGA
    CCCCCTGGCC CCCCATTTAC TGCACATGAA ATCAACAAGG GACACCCTAA CCTAGCGGCT
    ACCCCGCCGG GTCATGCCTC TTCCCCTGGA CTATCTCAGG TATCAGTTTC AACAGTATCC
    ACTGCGCATC TCTATGGTCA TCCTAAAGGC TGGGAGCCAG GAGGAGGGTC TCCGTACACA
    ACGGGACAGA ATGCCGGCAC GACCCCTCTG GTATACTCTC CAACAACCCA GCCAATGAAC
    GCACAGCCTC AGAGCCGCCC GTTTGCCCCT GGGCCTCGTC CTACCCATCA CCAGTTTTTC
    CAGAGGACGC AGATGCAAAC GGCCCGGCCC ACCATTCCAA GCAACACCCC ACCCATTCGG
    CCCACGTCTC AGACCCCCAC GGCAGCAGTA TATTCTCCCA ATCAGCACAT CATGATGACC
    ATGGCTCACA TGCCCTTTCA TTCCCCACAG ACAGCGCAGT ACTACATCCC TCAGTATCGT
    CACAGTGCTC CACAGTATGT GGGACCTCCT CAACAGTACC CTGTCCAGCC GACTGGACCC
    AGTACCTTCT ATGCTGCAGC CAGTCCAGGG GAGTTTCCTG CACCTTATGC AGCAGGACCT
    CCCTACTATC CTGGGCAGCC CGTGTACACA CCATCTCCAC CCATTATAGT GCCTACACCA
    CAACAACCTC CACCTGCTAA GAGGGAAAAG AAAACAATCC GCATCCGAGA CCCTAACCAG
    GGTGGCAAGG ATGTCACAGA CGAAATATTA TCAGGAGTGG GTTTGAGTCG AAACCCAACC
    CCGCCTGTGG GACGGCCCTC TTCCACTCCT ACACCTCCAC AGCAGCTGAA CAGCCAGGTA
    GCAGATCATG GGCACATCAT GTATAATGTG GACAGCAGCC CGCACCTTCC AGCATCCTTC
    AACTTGAAAG CAGACGATAA GCCAAAACTA GAGTTTTCCT TGCAAAGAAC TGCATCTCCT
    GGTCTGCGGC AGCCCGACAC TCCTCTGGAG AGAAGAGATC CCAGCAGCCC AGTCCAAACA
    CCCTCCTCTC CCCCACACAA ACCTGAGCTT CCCCCCTCCG ACTCAGAGAC GGCCTCAAGT
    GTAGCTACAG CCCCGACTCC TTCCATACCT GCCTCAACAG AAGAGTCAGC TGATGCCCCG
    AGCCCATTAG CTGAGCCCAG TCTCACTAAA GCTATCACAC CTGAACCAGA GAGCTCTGAG
    CCAGAGAAAT CCTCCTCACC ACCACCTCAA AGTCTGTCTG GATCCCTTAC CCAGCACGAA
    AAAGCCGTCA ATGGGCTCAC GGATGTTGAC GCTGCTCCAC TGAGTGAAGA ATTGGAAACC
    CAACCCAGGG AAGCCTCTCC ATTGCTGCCT ACTTCCAGTG TGCCCCAGTC AGAGCCCAGG
    CCAGTCACAC CGGTGCTGGA GGAAGAGTCT GATCCAATCA ATATGAACAG TCCTTTGCCT
    CCTGTTGAGG ACGATGCTGG ATGTCCTGAC AACGTTTCCC CCTCCCTCTC CACCTCCACG
    ACTGCTGCTA TCTCCACCAC TCCTCCTGCT CCTCCTCCAG GACTCTCGCA CCCCAGTCAG
    GTTTCTGCAG CGTTGGACAG GAGACCCTCT AATGGAGCAG AGATCAAAGA AACAGGGAAG
    GAAAACGAGG CCTTGCCGGA CAAGAGAGGA GAGCCTTTCT TACAGTCCAG AAAAAGCTCA
    AATCAAGCTA CATCTAGTGC ACCAAAGACC TGGAAGAAGC CAAAAGAGGA CATGCCTGTA
    GGGCAAGATC AGTGTCCAGA CAAAGAGGAG GACACTGATC AGGCCAGTAT GGAACCTGCT
    CCTGAAAGCC CCAGTCCTCC TCCTGTGGAG GTGGAGAGGA GGCCATCAGT AGGAGCAGTG
    CTCGAGGAGA ATGGAGAACA GGAAGTGGAG CCTTTGAGAA ACGGTGCTGA ACACACTTCT
    GAAACTGAGA GCAGTGACAG CATCCACCCG CCTGCAGTCA AAGAGCCGTT GATCAAAGTA
    TTGCCACAGC CTGCAGAGAA GAATGGAAAG AAGCAGTACA ACAGAGACTT CCTGCTGGGC
    TTCCAGTTTA TGCCTGCATG TGTGCAGAAA CCAGAAGGGC TTCCTCCCAT TTCTGATGTT
    GTTCTAGATA AGATTAACCA AAATAAGCTG CCCATGCGGC CGGTAGACCC ACGTGTGCTT
    TCCAGAGGCC CGGACTTTAC GCCAGCGTTT GCGGATTTCA GCCGACCCAT TTCAGGAGGC
    AGAGGGGCAC CGTTGCTGAA TGTTGGCATG CGACGGCCAC CTGGACGCAA GATCATCACA
    AACGTGTCTG TGATTGATGA GGTGCAGCTG AAGACAACAG AGAATGCCTG GAAGCCTGGC
    ATGAAGCGGG AAGGTGCAGT TGAAGATCCA GATGCCCAGA GAACTCAGGA GCTTTTCCGA
    AAAGTGCGCA GTATTCTCAA TAAACTGACA CCGCAGATGT TCAGCCAGCT GATGAAGCAG
    GTGACTGACC TCACCATCGA CACAGAGGAG CGACTCAAGG GTGTCATAGA TCTGGTCTTT
    GAAAAAGCAA TCAATGAGCC CAGCTTCTCT GTGGCCTACG GCAACATGTG CAGCTGTCTG
    GCCACGTTAA AAGTGCCCAT GACGGATAAG CCCAACAGCA CTGTGAATTT CCGGAAGCTG
    CTTCTGAACC GCTGTCAGAA AGAGTTTGAG AAGGACAAGA TGGATGACGA TGCCTTTGAG
    AAGAAACACA GGGAGCTGGA GGCAGCTACT GCGTCCAGTG AGCGGGAGAG GCTTCAGGAG
    GAGCTGGAAG AGGCCAAAGA CAAGGCCCGA AGACGCTCCA TTGGCAACAT TAAGTTCATC
    GGCGAGCTCT TCAAGCTGCG GATGCTGACA GAGGCCATCA TGCATGACTG CGTGGTGAAG
    TTGCTGAAAA ACCATGATGA GGAATCACTG GAGTGCCTGT GTAGACTGCT CACCACTATT
    GGAAAGGACC TGGACTTTGA GAAAGCTAAG CCACGAATGG ATCAGTACTT TAATCAGATG
    GAGAAGATAG TTAAGGAGAG GAAGACTTCT TCACGCATTC GCTTCATGCT GCAGGATGTT
    ATTGATCTGC GATTGCACAA CTGGGTTTCT CGAAGAGCAG ATCAGGGCCC CAAGACCATC
    GAGCAGATCC ATAAAGACGC AAAGCTGGAG GAGCAGGAGG AACAGAGGAA AGTCCACCAG
    CAACTGCTTT CCAAAGACAA CAAGAGGAGA CCAGTGGTGC AGAGAGAGGA GACCTGGAGC
    ACTGTGCCTA TGACTAAGAA CAGCAGAACA ATAGACCCCG CCAAGATTCC CAAATTCTCC
    AAGCCAGCGA TAGATGAGAA GATTCAGCTG GGGCCGAGAG CTCAGGTGAA CTGGATGAAG
    GGCAGCAGTG GTGGAGCTGG AGCAAAGGCC AGTGAGTCAG ATGCGTCTCG GCCGAGTGCC
    AGTTTGAATC GTTACTCTCC TCTGCAACCC TCGGCACTCC AGACTTCTTC TTTACCCTCC
    ACATCTCCAG ATTTTGATTC CAGGAGAGTT CTCGGCAGTC GTGGAAGCTC AGGACGGGAG
    CGCAATGACA AGCCATTGAG TGCGGGTCCT GCTCGGACAG GGCCTATTTC ATTGAGCAGT
    AGCAATAAGG AGACCCCCGA AGAGCTGGTT CAGGAAGTGT CCCGCAGAGA CTCCAATGCT
    TCAGATACAC CCAAGCTGCT CGTCAGTACT GCAGACAAGA GCAGACTGGA GAACAGCCAA
    CCCAGAGAGT CTGCTGTTAA ACTTGAAGCA CTCTCAGGGC CCTCTCCAGA TAAACCTGCA
    TTATCAGAAG AGGAGATGGA GAGGCGGTCC AAGTCTATAA TTGATGAGTT TTTGCACATT
    AATGATTACA AGGAAGCTTT GCAGTGTGTT GAGGAACTAG AGCAGTCTGC CATGCTCTAT
    GTGTTTGTGC GGGTGGGTGT GGAGTCCACG CTGGAAAGGA GTCAGATCAC GCGAGACCAT
    ATGGGTCAGC TGCTCTTCCA GCTTCTGCAG GCTGGAGTCC TCCCTAAACT TCAGTTCTTT
    AAAGGGTTTT CTGAAACTCT TGAATTAGCA GATGATATGG CCATTGATAT TCCCCATATA
    TGGCTGTACC TTGCGGAGCT GGTAACCCCT GTGCTTCGAG AGGGGGGAAT CTCTATGAGA
    GAATTATTTA GTGAATTTAG CAAACCATTA CTCCCTGTGG GAAGAGCTGG GATATTATTT
    TCTGAAATAT TGCACCTTCT ATGCAAACAA ATGAGCCATA AGAAAGTGGG ATCATTATGG
    AGGGATTCTG GGTTGAGCTG GGCAGATTTT ATACCTGAAA CAGAGGATGT GCACAGCTTC
    ATCACAGAAC ATAAACTAGA GTTCACTCTG TCTGATGGCT CAAACCCGAC CGAGGCTGTC
    TCTAAGAGAA CACTCTCGCC TGAGGAACTG AACAAGCAAC TTGAAAAACT TTTACTTGAG
    GACATGGTTG GCGATGAACA AATCTTCGAC TGGGTTGAGG CCAATCTAGA TGAATCAGAA
    ATGAGTTCAG CTCCTTTCGT CAGAGCTTTA ATGACTGCTG TGTGTAAAGC AGCAGTAAAA
    ACTGAGGGCT CCTCGTGTAA AGTGGACTTG TCCATAATTC AGACGAGATT GCCTGTATTA
    CACAAGTACC TTAACTCAGA CACAGAGAGA CAGTTGCAAG CACTTTATGC ACTTCAAGCA
    TTGATAGTAA AACTGGACCA GCCTGCTAAT TTGCTCCGGA TGTTTTTTGA CTGTTTGTAT
    GATGAGGACG TGATCTCTGA AGACGCTTTC TATAAATGGG AGGTCAGTAA AGACCCCGCT
    GAACAGCAAG GCAAAGGTGT TGCCCTGAAG TCCGTCACAG CCTTCTTCAC GTGGCTGCGC
    GAGGCCGAGG AAGAGTCCGA GGACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.