col17a1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	col17a1a
Entrez Gene ID	557257
Full Name	collagen, type XVII, alpha 1a
Synonyms	si:ch211-281g13.3
General protein information	Preferred Names collagen, type XVII, alpha 1a Names collagen, type XVII, alpha 1a col17a1 col17a1b novel collagen protein
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005160111.4	XP_005160168.1	collagen, type XVII, alpha 1a isoform X1
XM_017354917.1	XP_017210406.1	collagen, type XVII, alpha 1a isoform X2
XM_005160111.3	XP_005160168.1	collagen, type XVII, alpha 1a isoform X1
XM_005160112.3	XP_005160169.1	collagen, type XVII, alpha 1a isoform X1
XM_005160112.4	XP_005160169.1	collagen, type XVII, alpha 1a isoform X1
XM_017354917.2	XP_017210406.1	collagen, type XVII, alpha 1a isoform X2
XM_021474972.1	XP_021330647.1	collagen, type XVII, alpha 1a isoform X3
NM_001145565.1	NP_001139037.1	collagen, type XVII, alpha 1a

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

A COL17A1 Splice-Altering Mutation Is Prevalent in Inherited Recurrent Corneal Erosions.
Oliver VF, van Bysterveldt KA, Cadzow M, Steger B, Romano V, Markie D, Hewitt AW, Mackey DA, Willoughby CE, Sherwin T, Crosier PS, McGhee CN, Vincent AL
Ophthalmology123(4)709-22(2016 Apr)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Zebrafish: a model system to study heritable skin diseases.
Li Q, Frank M, Thisse CI, Thisse BV, Uitto J
The Journal of investigative dermatology131(3)565-71(2011 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following col17a1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col17a1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa34303	NM_001145565.1 Latest version!	Danio rerio collagen, type XVII, alpha 1a (col17a1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa06151	XM_005160112.4 Latest version!	Danio rerio collagen, type XVII, alpha 1a (col17a1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa06151	XM_005160111.4 Latest version!	Danio rerio collagen, type XVII, alpha 1a (col17a1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa06152	XM_017354917.2 Latest version!	Danio rerio collagen, type XVII, alpha 1a (col17a1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa147251	XM_021474972.1 Latest version!	Danio rerio collagen, type XVII, alpha 1a (col17a1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
View Old Accession Versions >>
ODa06151	XM_005160111.3	Danio rerio collagen, type XVII, alpha 1a (col17a1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa06151	XM_005160112.3	Danio rerio collagen, type XVII, alpha 1a (col17a1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa06152	XM_017354917.1	Danio rerio collagen, type XVII, alpha 1a (col17a1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

col17a1a ( NM_001145565.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001139037.1 Danio rerio collagen, type XVII, alpha 1a (col17a1a), mRNA.

Price & Availability↑

CloneID	ODa34303
Clone ID Related Accession (Same CDS sequence)	NM_001145565.1
Accession Version	NM_001145565.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-23
Organism	Danio rerio(zebrafish)
Product	collagen, type XVII, alpha 1a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX321880.9. On Mar 5, 2009 this sequence version replaced XM_680295.3. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA3505370, SAMEA3505371 [ECO:0000350] ##Evidence-Data-END##

ATGGACAAGC TAACAACAAC CAAGATTGTT AAAGTTGAGG GGGGATCTGG AGGATCTGGA 
AAAGAAATCC AGAAACATAC AGAAACTGTG ACGACTACAA GACTGACATC TCTACCGCCA 
AAAGCCCACA GCGGCCAGCA GATACTGACC TCCATCGGGA ACGGCTCAGG ATCTGCAGGA 
GGAGCTGTTT TGCTGGAGAA GAAGTTCATC ACTCACAGCA GCGCTGAAGG AGGAGCTTCC 
AGGAGTTATG TCATATCATC CTCCTCATCT GGAAGTTCAG CCAGCAGCAG CTCAGGAGCA 
GGATCTGTGA TCAGTGGATT TGACGGTATA TCTGGCGAAA TCAGTGTAAC CGGACCGATC 
ACTAGCTCTA CGGTTAAAAG CTCTACATCT GCAGTTAAAA GCTCTAGCGT GGTCTCTACC 
TCCATCACAT CCTCGTCAGC CGCAGGCGGA GAGCTTTCCT CTGGATACGG CTCCATCTCC 
TCATCCTCCT CCGAGTTCGT GTCTGGTTCC TCAGGAGGAT CCATCAGTAC TTCTGAAGGC 
AGCATGAGTA AATCTAGAAG CGGTGGAGGA TCATCTGCAG TCATGTCTGG CGAAGGAACA 
TCCAGCTCCA AGACTGTGTC CAAATTCAGC TCCTCGTCCC CTAGTGGAAC AAGGAAAGGT 
TTGAGTTACA GCACAGTTCC TACTGAGAGG AAGACCATTA TGAACATTAC CACAGGAGGA 
TATGAGGGGA GCTCCAGTGG CAATTCCTCC CCTGAGTACA TAAAGAAAGA CAGTGCTGCC 
ACCAGAGGGC GCACTCAAAC ACGAGAGAGT GAGATCAGAG CCAGACTGCA GAGCGCCTCG 
CCCTCCGCTA GATGGACAGA GTTGGATGAT GTGAAGAGAT TACTGAAGGG AAGTCGCTCT 
GGGAGCATCA GTCCACCACG TTCTCCCACC AGCACTCTCC CTATCCCCAA AAAAGCCAGC 
ATTGACGTAC GCCACGAGAG CCAGTCAGGC TTTGATGGCA CAGTTTTGGA TGCTGGATTC 
AGTGGATATT ACACCAATCC CAACAATCTC AGTTACACCA CATTGCAACA ACCATCATCA 
CCCACAGGCG GCATGCAAAA CAATCTGACC CTCAGTTCAT CTGCCCTTGA TACTACAGTG 
TATGGGATGC AGAATAACCT GTCTACATCT GGCGGTGCTA TGATGGCTAA TGGAAGGTGC 
ACTGGCTATG TGTATGGGGT CCCGAAAAAT GTTGGCAGCG CTGGATTGGC CACTACTATT 
TCTGCTATTC CAAGCAGTCC TATCACCGCT GATGACGTGT TTATGAAAGA TGGCAAGTTA 
ATTGCGATTG GGAAGGAAAA TGTAGCGGCT AAGACAGAGA CCGAGAGGCT GATCATGTCC 
AAAAACACTG GCAAACAGTT CGTCCCTTCA TCCTTCAATG CTGGAGCAGA CACGTTTTCT 
GAAGATTCAC TCAAGAGGGA GAAAAAGATG ATCTCGAGCG CCACAGATCC ACAGACCACC 
ACCATTACTG GTTCCAAATC CTCAACAAAA AACAAAGCAA CATATGCAGA TATTCGTAAA 
GATGATTCCG ACGGCGCTGG ATGTGGATTC TGGACGTGTT GCTCGTGGTG GAAATGGCTT 
CTGGCTTTGT TATTTGCTCT TCTCCTTCTG CTGGGACTCC TCTGTGGACT GATCGCACTA 
GGTGAGCAGC TGAGGAGGCT GAAGGCTCGT GTGGATGCTA TCGATGGTGG AGGAACCTCT 
CCTCGAACCA GCGGTTTGAA AACTGACGAC ATTCCTGGGG TCCAATCTGG TAACACGATA 
AACCTGGGTT ATGAACCTGC TGGGCAAGAT TCGTTGATCC TGCAGAGAGC TATCCTGAGA 
GAGCTGCAGT CGGACACCAT CAGAGCATCG CTGGCAACTT CGCTCAGGGG AGAGAGAGGA 
GAACCTGGAC CTAAAGGAGA CCAAGGGCCT CCTGGAGTTA AAGGTGATTC AGGATTTCCC 
GGGATTCCAG GTCCTCCTGG TCTTATTGGT CATCCTGGAC AGGAAGGCCC TCGAGGACCC 
AAAGGGAGCG CAGGTGAATC AGGAAGTGAT GGTTTACCTG GACCGAGGGG TCGTGAAGGT 
CCAGCGGGTC CCAGAGGAGA ACCAGGACCC CCAGGAATTG GGGAGAAAGG AGAGAAAGGC 
TCATTTGGTG ATGTTGGCGC TCCTGGGATA GCAGGACCAC CAGGACCCGT AGGGCCCAAA 
GGTGATGCAG GTGCACAAGG AGTACCGGGA CTTACAGGAG CACCTGGACC CCAAGGCTTC 
CGCGGAGATT CTGGTGATCC CGGACCCAAG GGTGATAAAG GACCTGCTGG GCCACCTGGG 
GTCAAAGGTG ATCAGGGAGA GAGAGGACCA CGTGGCGCCA CTGGTGAGCC GGGTCAGCCT 
GGACCAGCAG GTCCTCCTGG TGAGAAAGGT CTTAAGGGAT CAACAGGTGC ACCAGGACCA 
GATGGTGTTA AAGGACTTAG GGGTGACCAA GGACCCGTTG GGTTGCCTGG TGCTCGAGGA 
CCATCAGGAC CTCCCGGAGA TTCAGGAATA CCAGGAACAC CAGGACTCCA AGGACCACCA 
GGACTGCCAG GTACACCAGG CCAACCAGGA GCTAAAGGTG AGGCCGGTGA GCCTGGAAGA 
GTCATTTCTG CAGGCGCTAA TGCTAACACG GTGGCTGTAT CTGGACCACC AGGACCTCCT 
GGAGCTCCAG GTCCTGCTGG TCCACCTGGA CTATCAGGTC CTATTGGTCC TGCTGGTCTT 
CCTGGTTCAC CTGGTGCAAA GGGAGATAAA GGAGACAAAG GAGAGGATGG GAAACCTGGA 
GTCACTGAGA GTGCAGGGAA GAAATCAGAA GCAGGGGAAC CCGGTCCTCC AGGACCACCA 
GGACCTCCAG GACCACCCGG GGATGATGGA AAGCCAGGTG TTGGAGAACC TGGTCCAGCT 
GGTCCTCCTG GTGAAAAAGG TGAACCTGGA AGCTTTGTAC CTACTTCAGA AACATTCTTT 
GCTGGACCAC CTGGGCCTCC TGGACCTATT GGACCAAAGG GATCACAAGG TGATGTTGGC 
CCTCAAGGTT TCCCTGGTCA ACCTGGATTA CCTGGAGAAC CTGGAATTGG TATTCCAGGA 
CAGCCGGGAT CACCAGGACC ACGTGGAGAG CAAGGTCTTG ATGGTCCACT TGGACCACAG 
GGACCTCAAG GGCCAATGGG TCCCATGGGC CCAGAAGGTC CAAAAGGTGA TGCTGGAGTC 
CCAGGTGCCC CTGGTATTCC AGGTTATTCT TACGGTGGAG GATCCAGAAG TGCTCCGGGA 
CCACCAGGTC CACCTGGACC CCCAGGGCCT CCTGGAGAAC CAGGTTCTCA AGGTGACGGG 
TCACCGAATG CTGTTCAACA GATTTCAGAC TACCTCAGAA GTGAGCCCAT TAGACAATAC 
CTAGCTGGAC CTCCAGGCCC GCCTGGACCA CCAGGTCCCT CTGGATCAGC CTCTGATGAT 
GCTCTGGCTA GCAGAGTCAT TGAATATCTT CAACGAGATG AGGTGAGACA GTACCTGATT 
GGTCCTCCAG GACCCCCGGG ACCACCAGGT GTGCCAGGTG GATATGGCTT TAACACTCAG 
GAAGTGGCAG GACGAGTGAT GAGCCTTATT AACGATGCTG GTTTTAGAGG TTTACCAGGA 
CCTCCCGGCC CACCAGGACC TCAAGGACCT CCTGGAAACT CTGGAGGACT GGTGTCTTAC 
GCTGCTAACG AAAACCAGCA GCACATCCGC GCAGAGCCGC AGGAGTACAT CAAGAGCCAT 
CCAAGGGCTG ATGAGAATTT GGATTACACA GCCATCGCTA AACAGGTTTC AGATTACATT 
AAGGCTCATG GCCTGTTAAA TGGAAATGCT GGCTTTCCAG GTGCACCAGG TCGTCAAGGA 
TATCCTGGAC CACCAGGGTT TCCAGGAGAG CCCGGAACAC CAGGACAGCC TGGACCACCA 
GGACAGGCTG GACCACCAGG ACTGCCTGGA CCACCAGGAC TCGACACTGT ACACAATTAC 
TCCAACATGA CTGCTGTCTT GGACTACATC AAAGAAAATC ATATGAAAGG ACAAAAAGGC 
GACCTGGGAT TTCCAGGAAT TCCAGGATCC CCAGGTATTG ATGGTAAACA AGGCATAGTA 
GGCTTACCAG GCATAAAGGG ACAAAAAGGA GAAAGAGGTG AAGCAACATA CGTCAACCGA 
AGGAGGAGGA GGAGCACAGT AATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139037.1
CDS	177..4403
Translation	MDKLTTTKIVKVEGGSGGSGKEIQKHTETVTTTRLTSLPPKAHSGQQILTSIGNGSGSAGGAVLLEKKFITHSSAEGGASRSYVISSSSSGSSASSSSGAGSVISGFDGISGEISVTGPITSSTVKSSTSAVKSSSVVSTSITSSSAAGGELSSGYGSISSSSSEFVSGSSGGSISTSEGSMSKSRSGGGSSAVMSGEGTSSSKTVSKFSSSSPSGTRKGLSYSTVPTERKTIMNITTGGYEGSSSGNSSPEYIKKDSAATRGRTQTRESEIRARLQSASPSARWTELDDVKRLLKGSRSGSISPPRSPTSTLPIPKKASIDVRHESQSGFDGTVLDAGFSGYYTNPNNLSYTTLQQPSSPTGGMQNNLTLSSSALDTTVYGMQNNLSTSGGAMMANGRCTGYVYGVPKNVGSAGLATTISAIPSSPITADDVFMKDGKLIAIGKENVAAKTETERLIMSKNTGKQFVPSSFNAGADTFSEDSLKREKKMISSATDPQTTTITGSKSSTKNKATYADIRKDDSDGAGCGFWTCCSWWKWLLALLFALLLLLGLLCGLIALGEQLRRLKARVDAIDGGGTSPRTSGLKTDDIPGVQSGNTINLGYEPAGQDSLILQRAILRELQSDTIRASLATSLRGERGEPGPKGDQGPPGVKGDSGFPGIPGPPGLIGHPGQEGPRGPKGSAGESGSDGLPGPRGREGPAGPRGEPGPPGIGEKGEKGSFGDVGAPGIAGPPGPVGPKGDAGAQGVPGLTGAPGPQGFRGDSGDPGPKGDKGPAGPPGVKGDQGERGPRGATGEPGQPGPAGPPGEKGLKGSTGAPGPDGVKGLRGDQGPVGLPGARGPSGPPGDSGIPGTPGLQGPPGLPGTPGQPGAKGEAGEPGRVISAGANANTVAVSGPPGPPGAPGPAGPPGLSGPIGPAGLPGSPGAKGDKGDKGEDGKPGVTESAGKKSEAGEPGPPGPPGPPGPPGDDGKPGVGEPGPAGPPGEKGEPGSFVPTSETFFAGPPGPPGPIGPKGSQGDVGPQGFPGQPGLPGEPGIGIPGQPGSPGPRGEQGLDGPLGPQGPQGPMGPMGPEGPKGDAGVPGAPGIPGYSYGGGSRSAPGPPGPPGPPGPPGEPGSQGDGSPNAVQQISDYLRSEPIRQYLAGPPGPPGPPGPSGSASDDALASRVIEYLQRDEVRQYLIGPPGPPGPPGVPGGYGFNTQEVAGRVMSLINDAGFRGLPGPPGPPGPQGPPGNSGGLVSYAANENQQHIRAEPQEYIKSHPRADENLDYTAIAKQVSDYIKAHGLLNGNAGFPGAPGRQGYPGPPGFPGEPGTPGQPGPPGQAGPPGLPGPPGLDTVHNYSNMTAVLDYIKENHMKGQKGDLGFPGIPGSPGIDGKQGIVGLPGIKGQKGERGEATYVNRRRRRSTVI

Target ORF information:

RefSeq Version	NM_001145565.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XVII, alpha 1a (col17a1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145565.1

ORF Insert Sequence:

ATGGACAAGC TAACAACAAC CAAGATTGTT AAAGTTGAGG GGGGATCTGG AGGATCTGGA 
AAAGAAATCC AGAAACATAC AGAAACTGTG ACGACTACAA GACTGACATC TCTACCGCCA 
AAAGCCCACA GCGGCCAGCA GATACTGACC TCCATCGGGA ACGGCTCAGG ATCTGCAGGA 
GGAGCTGTTT TGCTGGAGAA GAAGTTCATC ACTCACAGCA GCGCTGAAGG AGGAGCTTCC 
AGGAGTTATG TCATATCATC CTCCTCATCT GGAAGTTCAG CCAGCAGCAG CTCAGGAGCA 
GGATCTGTGA TCAGTGGATT TGACGGTATA TCTGGCGAAA TCAGTGTAAC CGGACCGATC 
ACTAGCTCTA CGGTTAAAAG CTCTACATCT GCAGTTAAAA GCTCTAGCGT GGTCTCTACC 
TCCATCACAT CCTCGTCAGC CGCAGGCGGA GAGCTTTCCT CTGGATACGG CTCCATCTCC 
TCATCCTCCT CCGAGTTCGT GTCTGGTTCC TCAGGAGGAT CCATCAGTAC TTCTGAAGGC 
AGCATGAGTA AATCTAGAAG CGGTGGAGGA TCATCTGCAG TCATGTCTGG CGAAGGAACA 
TCCAGCTCCA AGACTGTGTC CAAATTCAGC TCCTCGTCCC CTAGTGGAAC AAGGAAAGGT 
TTGAGTTACA GCACAGTTCC TACTGAGAGG AAGACCATTA TGAACATTAC CACAGGAGGA 
TATGAGGGGA GCTCCAGTGG CAATTCCTCC CCTGAGTACA TAAAGAAAGA CAGTGCTGCC 
ACCAGAGGGC GCACTCAAAC ACGAGAGAGT GAGATCAGAG CCAGACTGCA GAGCGCCTCG 
CCCTCCGCTA GATGGACAGA GTTGGATGAT GTGAAGAGAT TACTGAAGGG AAGTCGCTCT 
GGGAGCATCA GTCCACCACG TTCTCCCACC AGCACTCTCC CTATCCCCAA AAAAGCCAGC 
ATTGACGTAC GCCACGAGAG CCAGTCAGGC TTTGATGGCA CAGTTTTGGA TGCTGGATTC 
AGTGGATATT ACACCAATCC CAACAATCTC AGTTACACCA CATTGCAACA ACCATCATCA 
CCCACAGGCG GCATGCAAAA CAATCTGACC CTCAGTTCAT CTGCCCTTGA TACTACAGTG 
TATGGGATGC AGAATAACCT GTCTACATCT GGCGGTGCTA TGATGGCTAA TGGAAGGTGC 
ACTGGCTATG TGTATGGGGT CCCGAAAAAT GTTGGCAGCG CTGGATTGGC CACTACTATT 
TCTGCTATTC CAAGCAGTCC TATCACCGCT GATGACGTGT TTATGAAAGA TGGCAAGTTA 
ATTGCGATTG GGAAGGAAAA TGTAGCGGCT AAGACAGAGA CCGAGAGGCT GATCATGTCC 
AAAAACACTG GCAAACAGTT CGTCCCTTCA TCCTTCAATG CTGGAGCAGA CACGTTTTCT 
GAAGATTCAC TCAAGAGGGA GAAAAAGATG ATCTCGAGCG CCACAGATCC ACAGACCACC 
ACCATTACTG GTTCCAAATC CTCAACAAAA AACAAAGCAA CATATGCAGA TATTCGTAAA 
GATGATTCCG ACGGCGCTGG ATGTGGATTC TGGACGTGTT GCTCGTGGTG GAAATGGCTT 
CTGGCTTTGT TATTTGCTCT TCTCCTTCTG CTGGGACTCC TCTGTGGACT GATCGCACTA 
GGTGAGCAGC TGAGGAGGCT GAAGGCTCGT GTGGATGCTA TCGATGGTGG AGGAACCTCT 
CCTCGAACCA GCGGTTTGAA AACTGACGAC ATTCCTGGGG TCCAATCTGG TAACACGATA 
AACCTGGGTT ATGAACCTGC TGGGCAAGAT TCGTTGATCC TGCAGAGAGC TATCCTGAGA 
GAGCTGCAGT CGGACACCAT CAGAGCATCG CTGGCAACTT CGCTCAGGGG AGAGAGAGGA 
GAACCTGGAC CTAAAGGAGA CCAAGGGCCT CCTGGAGTTA AAGGTGATTC AGGATTTCCC 
GGGATTCCAG GTCCTCCTGG TCTTATTGGT CATCCTGGAC AGGAAGGCCC TCGAGGACCC 
AAAGGGAGCG CAGGTGAATC AGGAAGTGAT GGTTTACCTG GACCGAGGGG TCGTGAAGGT 
CCAGCGGGTC CCAGAGGAGA ACCAGGACCC CCAGGAATTG GGGAGAAAGG AGAGAAAGGC 
TCATTTGGTG ATGTTGGCGC TCCTGGGATA GCAGGACCAC CAGGACCCGT AGGGCCCAAA 
GGTGATGCAG GTGCACAAGG AGTACCGGGA CTTACAGGAG CACCTGGACC CCAAGGCTTC 
CGCGGAGATT CTGGTGATCC CGGACCCAAG GGTGATAAAG GACCTGCTGG GCCACCTGGG 
GTCAAAGGTG ATCAGGGAGA GAGAGGACCA CGTGGCGCCA CTGGTGAGCC GGGTCAGCCT 
GGACCAGCAG GTCCTCCTGG TGAGAAAGGT CTTAAGGGAT CAACAGGTGC ACCAGGACCA 
GATGGTGTTA AAGGACTTAG GGGTGACCAA GGACCCGTTG GGTTGCCTGG TGCTCGAGGA 
CCATCAGGAC CTCCCGGAGA TTCAGGAATA CCAGGAACAC CAGGACTCCA AGGACCACCA 
GGACTGCCAG GTACACCAGG CCAACCAGGA GCTAAAGGTG AGGCCGGTGA GCCTGGAAGA 
GTCATTTCTG CAGGCGCTAA TGCTAACACG GTGGCTGTAT CTGGACCACC AGGACCTCCT 
GGAGCTCCAG GTCCTGCTGG TCCACCTGGA CTATCAGGTC CTATTGGTCC TGCTGGTCTT 
CCTGGTTCAC CTGGTGCAAA GGGAGATAAA GGAGACAAAG GAGAGGATGG GAAACCTGGA 
GTCACTGAGA GTGCAGGGAA GAAATCAGAA GCAGGGGAAC CCGGTCCTCC AGGACCACCA 
GGACCTCCAG GACCACCCGG GGATGATGGA AAGCCAGGTG TTGGAGAACC TGGTCCAGCT 
GGTCCTCCTG GTGAAAAAGG TGAACCTGGA AGCTTTGTAC CTACTTCAGA AACATTCTTT 
GCTGGACCAC CTGGGCCTCC TGGACCTATT GGACCAAAGG GATCACAAGG TGATGTTGGC 
CCTCAAGGTT TCCCTGGTCA ACCTGGATTA CCTGGAGAAC CTGGAATTGG TATTCCAGGA 
CAGCCGGGAT CACCAGGACC ACGTGGAGAG CAAGGTCTTG ATGGTCCACT TGGACCACAG 
GGACCTCAAG GGCCAATGGG TCCCATGGGC CCAGAAGGTC CAAAAGGTGA TGCTGGAGTC 
CCAGGTGCCC CTGGTATTCC AGGTTATTCT TACGGTGGAG GATCCAGAAG TGCTCCGGGA 
CCACCAGGTC CACCTGGACC CCCAGGGCCT CCTGGAGAAC CAGGTTCTCA AGGTGACGGG 
TCACCGAATG CTGTTCAACA GATTTCAGAC TACCTCAGAA GTGAGCCCAT TAGACAATAC 
CTAGCTGGAC CTCCAGGCCC GCCTGGACCA CCAGGTCCCT CTGGATCAGC CTCTGATGAT 
GCTCTGGCTA GCAGAGTCAT TGAATATCTT CAACGAGATG AGGTGAGACA GTACCTGATT 
GGTCCTCCAG GACCCCCGGG ACCACCAGGT GTGCCAGGTG GATATGGCTT TAACACTCAG 
GAAGTGGCAG GACGAGTGAT GAGCCTTATT AACGATGCTG GTTTTAGAGG TTTACCAGGA 
CCTCCCGGCC CACCAGGACC TCAAGGACCT CCTGGAAACT CTGGAGGACT GGTGTCTTAC 
GCTGCTAACG AAAACCAGCA GCACATCCGC GCAGAGCCGC AGGAGTACAT CAAGAGCCAT 
CCAAGGGCTG ATGAGAATTT GGATTACACA GCCATCGCTA AACAGGTTTC AGATTACATT 
AAGGCTCATG GCCTGTTAAA TGGAAATGCT GGCTTTCCAG GTGCACCAGG TCGTCAAGGA 
TATCCTGGAC CACCAGGGTT TCCAGGAGAG CCCGGAACAC CAGGACAGCC TGGACCACCA 
GGACAGGCTG GACCACCAGG ACTGCCTGGA CCACCAGGAC TCGACACTGT ACACAATTAC 
TCCAACATGA CTGCTGTCTT GGACTACATC AAAGAAAATC ATATGAAAGG ACAAAAAGGC 
GACCTGGGAT TTCCAGGAAT TCCAGGATCC CCAGGTATTG ATGGTAAACA AGGCATAGTA 
GGCTTACCAG GCATAAAGGG ACAAAAAGGA GAAAGAGGTG AAGCAACATA CGTCAACCGA 
AGGAGGAGGA GGAGCACAGT AATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col17a1a ( XM_005160111.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160168.1 Danio rerio collagen, type XVII, alpha 1a (col17a1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa06151
Clone ID Related Accession (Same CDS sequence)	XM_005160111.4 , XM_005160111.3 , XM_005160112.3 , XM_005160112.4
Accession Version	XM_005160111.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen, type XVII, alpha 1a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160111.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAAGC TAACAACAAC CAAGATTGTT AAAGTTGAGG GGGGATCTGG AGGATCTGGA 
AAAGAAATCC AGAAACATAC AGAAACTGTG ACGACTACAA GACTGACATC TCTACCGCCA 
AAAGCCCACA GCGGCCAGCA GATACTGACC TCCATCGGGA ACGGCTCAGG ATCTGCAGGA 
GGAGCTGTTT TGCTGGAGAA GAAGTTCATC ACTCACAGCA GCGCTGAAGG AGGAGCTTCC 
AGGAGTTATG TCATATCATC CTCCTCATCT GGAAGTTCAG CCAGCAGCAG CTCAGGAGCA 
GGATCTGTGA TCAGTGGATT TGACGGTATA TCTGGCGAAA TCAGTGTAAC CGGACCGATC 
ACTAGCTCTA CGGTTAAAAG CTCTACATCT GCAGTTAAAA GCTCTAGCGT GGTCTCTACC 
TCCATCACAT CCTCGTCAGC CGCAGGCGGA GAGCTTTCCT CTGGATACGG CTCCATCTCC 
TCATCCTCCT CCGAGTTCGT GTCTGGTTCC TCAGGAGGAT CCATCAGTAC TTCTGAAGGC 
AGCATGAGTA AATCTAGAAG CGGTGGAGGA TCATCTGCAG TCATGTCTGG CGAAGGAACA 
TCCAGCTCCA AGACTGTGTC CAAATTCAGC TCCTCGTCCC CTAGTGGAAC AAGGAAAGGT 
TTGAGTTACA GCACAGTTCC TACTGAGAGG AAGACCATTA TGAACATTAC CACAGGAGGA 
TATGAGGGGA GCTCCAGTGG CAATTCCTCC CCTGAGTACA TAAAGAAAGA GTACGTTTCC 
ACTAGCAGTG CTGCCACCAG AGGGCGCACT CAAACACGAG AGAGTGAGAT CAGAGCCAGA 
CTGCAGAGCG CCTCGCCCTC CGCTAGATGG ACAGAGTTGG ATGATGTGAA GAGATTACTG 
AAGGGAAGTC GCTCTGGGAG CATCAGTCCA CCACGTTCTC CCACCAGCAC TCTCCCTATC 
CCCAAAAAAG CCAGCATTGA CGTACGCCAC GAGAGCCAGT CAGGCTTTGA TGGCACAGTT 
TTGGATGCTG GATTCAGTGG ATATTACACC AATCCCAACA ATCTCAGTTA CACCACATTG 
CAACAACCAT CATCACCCAC AGGCGGCATG CAAAACAATC TGACCCTCAG TTCATCTGCC 
CTTGATACTA CAGTGTATGG GATGCAGAAT AACCTGTCTA CATCTGGCGG TGCTATGATG 
GCTAATGGAA GGTGCACTGG CTATGTGTAT GGGGTCCCGA AAAATGTTGG CAGCGCTGGA 
TTGGCCACTA CTATTTCTGC TATTCCAAGC AGTCCTATCA CCGCTGATGA CGTGTTTATG 
AAAGATGGCA AGTTAATTGC GATTGGGAAG GAAAATGTAG CGGCTAAGAC AGAGACCGAG 
AGGCTGATCA TGTCCAAAAA CACTGGCAAA CAGTTCGTCC CTTCATCCTT CAATGCTGGA 
GCAGACACGT TTTCTGAAGA TTCACTCAAG AGGGAGAAAA AGATGATCTC GAGCGCCACA 
GATCCACAGA CCACCACCAT TACTGGTTCC AAATCCTCAA CAAAAAACAA AGCAACATAT 
GCAGATATTC GTAAAGATGA TTCCGACGGC GCTGGATGTG GATTCTGGAC GTGTTGCTCG 
TGGTGGAAAT GGCTTCTGGC TTTGTTATTT GCTCTTCTCC TTCTGCTGGG ACTCCTCTGT 
GGACTGATCG CACTAGGTGA GCAGCTGAGG AGGCTGAAGG CTCGTGTGGA TGCTATCGAT 
GGTGGAGGAA CCTCTCCTCG AACCAGCGGT TTGAAAACTG ACGACATTCC TGGGGTCCAA 
TCTGGTAACA CGATAAACCT GGGTTATGAA CCTGCTGGGC AAGATTCGTT GATCCTGCAG 
AGAGCTATCC TGAGAGAGCT GCAGTCGGAC ACCATCAGAG CATCGCTGGC AACTTCGCTC 
AGGGGAGAGA GAGGAGAACC TGGACCTAAA GGAGACCAAG GGCCTCCTGG AGTTAAAGGT 
GATTCAGGAT TTCCCGGGAT TCCAGGTCCT CCTGGTCTTA TTGGTCATCC TGGACAGGAA 
GGCCCTCGAG GACCCAAAGG GAGCGCAGGT GAATCAGGAA GTGATGGTTT ACCTGGACCG 
AGGGGTCGTG AAGGTCCAGC GGGTCCCAGA GGAGAACCAG GACCCCCAGG AATTGGGGAG 
AAAGGAGAGA AAGGCTCATT TGGTGATGTT GGCGCTCCTG GGATAGCAGG ACCACCAGGA 
CCCGTAGGGC CCAAAGGTGA TGCAGGTGCA CAAGGAGTAC CGGGACTTAC AGGAGCACCT 
GGACCCCAAG GCTTCCGCGG AGATTCTGGT GATCCCGGAC CCAAGGGTGA TAAAGGACCT 
GCTGGGCCAC CTGGGGTCAA AGGTGATCAG GGAGAGAGAG GACCACGTGG CGCCACTGGT 
GAGCCGGGTC AGCCTGGACC AGCAGGTCCT CCTGGTGAGA AAGGTCTTAA GGGATCAACA 
GGTGCACCAG GACCAGATGG TGTTAAAGGA CTTAGGGGTG ACCAAGGACC CGTTGGGTTG 
CCTGGTGCTC GAGGACCATC AGGACCTCCC GGAGATTCAG GAATACCAGG AACACCAGGA 
CTCCAAGGAC CACCAGGACT GCCAGGTACA CCAGGCCAAC CAGGAGCTAA AGGTGAGGCC 
GGTGAGCCTG GAAGAGTCAT TTCTGCAGGC GCTAATGCTA ACACGGTGGC TGTATCTGGA 
CCACCAGGAC CTCCTGGAGC TCCAGGTCCT GCTGGTCCAC CTGGACTATC AGGTCCTATT 
GGTCCTGCTG GTCTTCCTGG TTCACCTGGT GCAAAGGGAG ATAAAGGAGA CAAAGGAGAG 
GATGGGAAAC CTGGAGTCAC TGAGAGTGCA GGGACAGAGA AATCAGAAGC AGGGGAACCC 
GGTCCTCCAG GACCACCAGG ACCTCCAGGA CCACCCGGGG ATGATGGAAA GCCAGGTGTT 
GGAGAACCTG GTCCAGCTGG TCCTCCTGGT GAAAAAGGTG AACCTGGAAG CTTTGTACCT 
ACTTCAGAAA CATTCTTTGC TGGACCACCT GGGCCTCCTG GACCTATTGG ACCAAAGGGA 
TCACAAGGTG ATGTTGGCCC TCAAGGTTTC CCTGGTCAAC CTGGATTACC TGGAGAACCT 
GGAATTGGTA TTCCAGGACA GCCGGGATCA CCAGGACCAC GTGGAGAGCA AGGTCTTGAT 
GGTCCACTTG GACCACAGGG ACCTCAAGGG CCAATGGGTC CCATGGGCCC AGAAGGTCCA 
AAAGGTGATG CTGGAGTCCC AGGTGCCCCT GGTATTCCAG GTTATTCTTA CGGTGGAGGA 
TCCAGAAGTG CTCCGGGACC ACCAGGTCCA CCTGGACCCC CAGGGCCTCC TGGAGAACCA 
GGTTCTCAAG GTGACGGGTC ACCGAATGCT GTTCAACAGA TTTCAGACTA CCTCAGAAGT 
GAGCCCATTA GACAATACCT AGCTGGACCT CCAGGCCCGC CTGGACCACC AGGTCCCTCT 
GGATCAGCCT CTGATGATGC TCTGGCTAGC AGAGTCATTG AATATCTTCA ACGAGATGAG 
GTGAGACAGT ACCTGATTGG TCCTCCAGGA CCCCCGGGAC CACCAGGTGT GCCAGGTGGA 
TATGGCTTTA ACACTCAGGA AGTGGCAGGA CGAGTGATGA GCCTTATTAA CGATGCTGGT 
TTTAGAGGTT TACCAGGACC TCCCGGCCCA CCAGGACCTC AAGGACCTCC TGGAAACTCT 
GGAGGACTGG TGTCTTACGC TGCTAACGAA AACCAGCAGC ACATCCGCGC AGAGCCGCAG 
GAGTACATCA AGAGCCATCC AAGGGCTGAT GAGAATTTGG ATTACACAGC CATCGCTAAA 
CAGGTTTCAG ATTACATTAA GGCTCATGGC CTGTTAAATG GAAATGCTGG CTTTCCAGGT 
GCACCAGGTC GTCAAGGATA TCCTGGACCA CCAGGGTTTC CAGGAGAGCC CGGAACACCA 
GGACAGCCTG GACCACCAGG ACAGGCTGGA CCACCAGGAC TGCCTGGACC ACCAGGACTC 
GACACTGTAC ACAATTACTC CAACATGACT GCTGTCTTGG ACTACATCAA AGAAAATCAT 
ATGAAAGGAC AAAAAGGCGA CCTGGGATTT CCAGGAATTC CAGGATCCCC AGGTATTGAT 
GGTAAACAAG GCATAGTAGG CTTACCAGGC ATAAAGGGAC AAAAAGGAGA AAGAGGTGAA 
GCAACATACG TCAACCGAAG GAGGAGGAGG AGCACAGTAA TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160168.1
CDS	186..4430
Translation	MDKLTTTKIVKVEGGSGGSGKEIQKHTETVTTTRLTSLPPKAHSGQQILTSIGNGSGSAGGAVLLEKKFITHSSAEGGASRSYVISSSSSGSSASSSSGAGSVISGFDGISGEISVTGPITSSTVKSSTSAVKSSSVVSTSITSSSAAGGELSSGYGSISSSSSEFVSGSSGGSISTSEGSMSKSRSGGGSSAVMSGEGTSSSKTVSKFSSSSPSGTRKGLSYSTVPTERKTIMNITTGGYEGSSSGNSSPEYIKKEYVSTSSAATRGRTQTRESEIRARLQSASPSARWTELDDVKRLLKGSRSGSISPPRSPTSTLPIPKKASIDVRHESQSGFDGTVLDAGFSGYYTNPNNLSYTTLQQPSSPTGGMQNNLTLSSSALDTTVYGMQNNLSTSGGAMMANGRCTGYVYGVPKNVGSAGLATTISAIPSSPITADDVFMKDGKLIAIGKENVAAKTETERLIMSKNTGKQFVPSSFNAGADTFSEDSLKREKKMISSATDPQTTTITGSKSSTKNKATYADIRKDDSDGAGCGFWTCCSWWKWLLALLFALLLLLGLLCGLIALGEQLRRLKARVDAIDGGGTSPRTSGLKTDDIPGVQSGNTINLGYEPAGQDSLILQRAILRELQSDTIRASLATSLRGERGEPGPKGDQGPPGVKGDSGFPGIPGPPGLIGHPGQEGPRGPKGSAGESGSDGLPGPRGREGPAGPRGEPGPPGIGEKGEKGSFGDVGAPGIAGPPGPVGPKGDAGAQGVPGLTGAPGPQGFRGDSGDPGPKGDKGPAGPPGVKGDQGERGPRGATGEPGQPGPAGPPGEKGLKGSTGAPGPDGVKGLRGDQGPVGLPGARGPSGPPGDSGIPGTPGLQGPPGLPGTPGQPGAKGEAGEPGRVISAGANANTVAVSGPPGPPGAPGPAGPPGLSGPIGPAGLPGSPGAKGDKGDKGEDGKPGVTESAGTEKSEAGEPGPPGPPGPPGPPGDDGKPGVGEPGPAGPPGEKGEPGSFVPTSETFFAGPPGPPGPIGPKGSQGDVGPQGFPGQPGLPGEPGIGIPGQPGSPGPRGEQGLDGPLGPQGPQGPMGPMGPEGPKGDAGVPGAPGIPGYSYGGGSRSAPGPPGPPGPPGPPGEPGSQGDGSPNAVQQISDYLRSEPIRQYLAGPPGPPGPPGPSGSASDDALASRVIEYLQRDEVRQYLIGPPGPPGPPGVPGGYGFNTQEVAGRVMSLINDAGFRGLPGPPGPPGPQGPPGNSGGLVSYAANENQQHIRAEPQEYIKSHPRADENLDYTAIAKQVSDYIKAHGLLNGNAGFPGAPGRQGYPGPPGFPGEPGTPGQPGPPGQAGPPGLPGPPGLDTVHNYSNMTAVLDYIKENHMKGQKGDLGFPGIPGSPGIDGKQGIVGLPGIKGQKGERGEATYVNRRRRRSTVI

Target ORF information:

RefSeq Version	XM_005160111.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XVII, alpha 1a (col17a1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160111.4

ORF Insert Sequence:

ATGGACAAGC TAACAACAAC CAAGATTGTT AAAGTTGAGG GGGGATCTGG AGGATCTGGA 
AAAGAAATCC AGAAACATAC AGAAACTGTG ACGACTACAA GACTGACATC TCTACCGCCA 
AAAGCCCACA GCGGCCAGCA GATACTGACC TCCATCGGGA ACGGCTCAGG ATCTGCAGGA 
GGAGCTGTTT TGCTGGAGAA GAAGTTCATC ACTCACAGCA GCGCTGAAGG AGGAGCTTCC 
AGGAGTTATG TCATATCATC CTCCTCATCT GGAAGTTCAG CCAGCAGCAG CTCAGGAGCA 
GGATCTGTGA TCAGTGGATT TGACGGTATA TCTGGCGAAA TCAGTGTAAC CGGACCGATC 
ACTAGCTCTA CGGTTAAAAG CTCTACATCT GCAGTTAAAA GCTCTAGCGT GGTCTCTACC 
TCCATCACAT CCTCGTCAGC CGCAGGCGGA GAGCTTTCCT CTGGATACGG CTCCATCTCC 
TCATCCTCCT CCGAGTTCGT GTCTGGTTCC TCAGGAGGAT CCATCAGTAC TTCTGAAGGC 
AGCATGAGTA AATCTAGAAG CGGTGGAGGA TCATCTGCAG TCATGTCTGG CGAAGGAACA 
TCCAGCTCCA AGACTGTGTC CAAATTCAGC TCCTCGTCCC CTAGTGGAAC AAGGAAAGGT 
TTGAGTTACA GCACAGTTCC TACTGAGAGG AAGACCATTA TGAACATTAC CACAGGAGGA 
TATGAGGGGA GCTCCAGTGG CAATTCCTCC CCTGAGTACA TAAAGAAAGA GTACGTTTCC 
ACTAGCAGTG CTGCCACCAG AGGGCGCACT CAAACACGAG AGAGTGAGAT CAGAGCCAGA 
CTGCAGAGCG CCTCGCCCTC CGCTAGATGG ACAGAGTTGG ATGATGTGAA GAGATTACTG 
AAGGGAAGTC GCTCTGGGAG CATCAGTCCA CCACGTTCTC CCACCAGCAC TCTCCCTATC 
CCCAAAAAAG CCAGCATTGA CGTACGCCAC GAGAGCCAGT CAGGCTTTGA TGGCACAGTT 
TTGGATGCTG GATTCAGTGG ATATTACACC AATCCCAACA ATCTCAGTTA CACCACATTG 
CAACAACCAT CATCACCCAC AGGCGGCATG CAAAACAATC TGACCCTCAG TTCATCTGCC 
CTTGATACTA CAGTGTATGG GATGCAGAAT AACCTGTCTA CATCTGGCGG TGCTATGATG 
GCTAATGGAA GGTGCACTGG CTATGTGTAT GGGGTCCCGA AAAATGTTGG CAGCGCTGGA 
TTGGCCACTA CTATTTCTGC TATTCCAAGC AGTCCTATCA CCGCTGATGA CGTGTTTATG 
AAAGATGGCA AGTTAATTGC GATTGGGAAG GAAAATGTAG CGGCTAAGAC AGAGACCGAG 
AGGCTGATCA TGTCCAAAAA CACTGGCAAA CAGTTCGTCC CTTCATCCTT CAATGCTGGA 
GCAGACACGT TTTCTGAAGA TTCACTCAAG AGGGAGAAAA AGATGATCTC GAGCGCCACA 
GATCCACAGA CCACCACCAT TACTGGTTCC AAATCCTCAA CAAAAAACAA AGCAACATAT 
GCAGATATTC GTAAAGATGA TTCCGACGGC GCTGGATGTG GATTCTGGAC GTGTTGCTCG 
TGGTGGAAAT GGCTTCTGGC TTTGTTATTT GCTCTTCTCC TTCTGCTGGG ACTCCTCTGT 
GGACTGATCG CACTAGGTGA GCAGCTGAGG AGGCTGAAGG CTCGTGTGGA TGCTATCGAT 
GGTGGAGGAA CCTCTCCTCG AACCAGCGGT TTGAAAACTG ACGACATTCC TGGGGTCCAA 
TCTGGTAACA CGATAAACCT GGGTTATGAA CCTGCTGGGC AAGATTCGTT GATCCTGCAG 
AGAGCTATCC TGAGAGAGCT GCAGTCGGAC ACCATCAGAG CATCGCTGGC AACTTCGCTC 
AGGGGAGAGA GAGGAGAACC TGGACCTAAA GGAGACCAAG GGCCTCCTGG AGTTAAAGGT 
GATTCAGGAT TTCCCGGGAT TCCAGGTCCT CCTGGTCTTA TTGGTCATCC TGGACAGGAA 
GGCCCTCGAG GACCCAAAGG GAGCGCAGGT GAATCAGGAA GTGATGGTTT ACCTGGACCG 
AGGGGTCGTG AAGGTCCAGC GGGTCCCAGA GGAGAACCAG GACCCCCAGG AATTGGGGAG 
AAAGGAGAGA AAGGCTCATT TGGTGATGTT GGCGCTCCTG GGATAGCAGG ACCACCAGGA 
CCCGTAGGGC CCAAAGGTGA TGCAGGTGCA CAAGGAGTAC CGGGACTTAC AGGAGCACCT 
GGACCCCAAG GCTTCCGCGG AGATTCTGGT GATCCCGGAC CCAAGGGTGA TAAAGGACCT 
GCTGGGCCAC CTGGGGTCAA AGGTGATCAG GGAGAGAGAG GACCACGTGG CGCCACTGGT 
GAGCCGGGTC AGCCTGGACC AGCAGGTCCT CCTGGTGAGA AAGGTCTTAA GGGATCAACA 
GGTGCACCAG GACCAGATGG TGTTAAAGGA CTTAGGGGTG ACCAAGGACC CGTTGGGTTG 
CCTGGTGCTC GAGGACCATC AGGACCTCCC GGAGATTCAG GAATACCAGG AACACCAGGA 
CTCCAAGGAC CACCAGGACT GCCAGGTACA CCAGGCCAAC CAGGAGCTAA AGGTGAGGCC 
GGTGAGCCTG GAAGAGTCAT TTCTGCAGGC GCTAATGCTA ACACGGTGGC TGTATCTGGA 
CCACCAGGAC CTCCTGGAGC TCCAGGTCCT GCTGGTCCAC CTGGACTATC AGGTCCTATT 
GGTCCTGCTG GTCTTCCTGG TTCACCTGGT GCAAAGGGAG ATAAAGGAGA CAAAGGAGAG 
GATGGGAAAC CTGGAGTCAC TGAGAGTGCA GGGACAGAGA AATCAGAAGC AGGGGAACCC 
GGTCCTCCAG GACCACCAGG ACCTCCAGGA CCACCCGGGG ATGATGGAAA GCCAGGTGTT 
GGAGAACCTG GTCCAGCTGG TCCTCCTGGT GAAAAAGGTG AACCTGGAAG CTTTGTACCT 
ACTTCAGAAA CATTCTTTGC TGGACCACCT GGGCCTCCTG GACCTATTGG ACCAAAGGGA 
TCACAAGGTG ATGTTGGCCC TCAAGGTTTC CCTGGTCAAC CTGGATTACC TGGAGAACCT 
GGAATTGGTA TTCCAGGACA GCCGGGATCA CCAGGACCAC GTGGAGAGCA AGGTCTTGAT 
GGTCCACTTG GACCACAGGG ACCTCAAGGG CCAATGGGTC CCATGGGCCC AGAAGGTCCA 
AAAGGTGATG CTGGAGTCCC AGGTGCCCCT GGTATTCCAG GTTATTCTTA CGGTGGAGGA 
TCCAGAAGTG CTCCGGGACC ACCAGGTCCA CCTGGACCCC CAGGGCCTCC TGGAGAACCA 
GGTTCTCAAG GTGACGGGTC ACCGAATGCT GTTCAACAGA TTTCAGACTA CCTCAGAAGT 
GAGCCCATTA GACAATACCT AGCTGGACCT CCAGGCCCGC CTGGACCACC AGGTCCCTCT 
GGATCAGCCT CTGATGATGC TCTGGCTAGC AGAGTCATTG AATATCTTCA ACGAGATGAG 
GTGAGACAGT ACCTGATTGG TCCTCCAGGA CCCCCGGGAC CACCAGGTGT GCCAGGTGGA 
TATGGCTTTA ACACTCAGGA AGTGGCAGGA CGAGTGATGA GCCTTATTAA CGATGCTGGT 
TTTAGAGGTT TACCAGGACC TCCCGGCCCA CCAGGACCTC AAGGACCTCC TGGAAACTCT 
GGAGGACTGG TGTCTTACGC TGCTAACGAA AACCAGCAGC ACATCCGCGC AGAGCCGCAG 
GAGTACATCA AGAGCCATCC AAGGGCTGAT GAGAATTTGG ATTACACAGC CATCGCTAAA 
CAGGTTTCAG ATTACATTAA GGCTCATGGC CTGTTAAATG GAAATGCTGG CTTTCCAGGT 
GCACCAGGTC GTCAAGGATA TCCTGGACCA CCAGGGTTTC CAGGAGAGCC CGGAACACCA 
GGACAGCCTG GACCACCAGG ACAGGCTGGA CCACCAGGAC TGCCTGGACC ACCAGGACTC 
GACACTGTAC ACAATTACTC CAACATGACT GCTGTCTTGG ACTACATCAA AGAAAATCAT 
ATGAAAGGAC AAAAAGGCGA CCTGGGATTT CCAGGAATTC CAGGATCCCC AGGTATTGAT 
GGTAAACAAG GCATAGTAGG CTTACCAGGC ATAAAGGGAC AAAAAGGAGA AAGAGGTGAA 
GCAACATACG TCAACCGAAG GAGGAGGAGG AGCACAGTAA TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col17a1a ( XM_005160111.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160168.1 Danio rerio collagen, type XVII, alpha 1a (col17a1a), transcript variant X1, mRNA.

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CloneID	ODa06151
Clone ID Related Accession (Same CDS sequence)	XM_005160111.4 , XM_005160111.3 , XM_005160112.3 , XM_005160112.4
Accession Version	XM_005160111.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	collagen, type XVII, alpha 1a isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160111.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAAGC TAACAACAAC CAAGATTGTT AAAGTTGAGG GGGGATCTGG AGGATCTGGA 
AAAGAAATCC AGAAACATAC AGAAACTGTG ACGACTACAA GACTGACATC TCTACCGCCA 
AAAGCCCACA GCGGCCAGCA GATACTGACC TCCATCGGGA ACGGCTCAGG ATCTGCAGGA 
GGAGCTGTTT TGCTGGAGAA GAAGTTCATC ACTCACAGCA GCGCTGAAGG AGGAGCTTCC 
AGGAGTTATG TCATATCATC CTCCTCATCT GGAAGTTCAG CCAGCAGCAG CTCAGGAGCA 
GGATCTGTGA TCAGTGGATT TGACGGTATA TCTGGCGAAA TCAGTGTAAC CGGACCGATC 
ACTAGCTCTA CGGTTAAAAG CTCTACATCT GCAGTTAAAA GCTCTAGCGT GGTCTCTACC 
TCCATCACAT CCTCGTCAGC CGCAGGCGGA GAGCTTTCCT CTGGATACGG CTCCATCTCC 
TCATCCTCCT CCGAGTTCGT GTCTGGTTCC TCAGGAGGAT CCATCAGTAC TTCTGAAGGC 
AGCATGAGTA AATCTAGAAG CGGTGGAGGA TCATCTGCAG TCATGTCTGG CGAAGGAACA 
TCCAGCTCCA AGACTGTGTC CAAATTCAGC TCCTCGTCCC CTAGTGGAAC AAGGAAAGGT 
TTGAGTTACA GCACAGTTCC TACTGAGAGG AAGACCATTA TGAACATTAC CACAGGAGGA 
TATGAGGGGA GCTCCAGTGG CAATTCCTCC CCTGAGTACA TAAAGAAAGA GTACGTTTCC 
ACTAGCAGTG CTGCCACCAG AGGGCGCACT CAAACACGAG AGAGTGAGAT CAGAGCCAGA 
CTGCAGAGCG CCTCGCCCTC CGCTAGATGG ACAGAGTTGG ATGATGTGAA GAGATTACTG 
AAGGGAAGTC GCTCTGGGAG CATCAGTCCA CCACGTTCTC CCACCAGCAC TCTCCCTATC 
CCCAAAAAAG CCAGCATTGA CGTACGCCAC GAGAGCCAGT CAGGCTTTGA TGGCACAGTT 
TTGGATGCTG GATTCAGTGG ATATTACACC AATCCCAACA ATCTCAGTTA CACCACATTG 
CAACAACCAT CATCACCCAC AGGCGGCATG CAAAACAATC TGACCCTCAG TTCATCTGCC 
CTTGATACTA CAGTGTATGG GATGCAGAAT AACCTGTCTA CATCTGGCGG TGCTATGATG 
GCTAATGGAA GGTGCACTGG CTATGTGTAT GGGGTCCCGA AAAATGTTGG CAGCGCTGGA 
TTGGCCACTA CTATTTCTGC TATTCCAAGC AGTCCTATCA CCGCTGATGA CGTGTTTATG 
AAAGATGGCA AGTTAATTGC GATTGGGAAG GAAAATGTAG CGGCTAAGAC AGAGACCGAG 
AGGCTGATCA TGTCCAAAAA CACTGGCAAA CAGTTCGTCC CTTCATCCTT CAATGCTGGA 
GCAGACACGT TTTCTGAAGA TTCACTCAAG AGGGAGAAAA AGATGATCTC GAGCGCCACA 
GATCCACAGA CCACCACCAT TACTGGTTCC AAATCCTCAA CAAAAAACAA AGCAACATAT 
GCAGATATTC GTAAAGATGA TTCCGACGGC GCTGGATGTG GATTCTGGAC GTGTTGCTCG 
TGGTGGAAAT GGCTTCTGGC TTTGTTATTT GCTCTTCTCC TTCTGCTGGG ACTCCTCTGT 
GGACTGATCG CACTAGGTGA GCAGCTGAGG AGGCTGAAGG CTCGTGTGGA TGCTATCGAT 
GGTGGAGGAA CCTCTCCTCG AACCAGCGGT TTGAAAACTG ACGACATTCC TGGGGTCCAA 
TCTGGTAACA CGATAAACCT GGGTTATGAA CCTGCTGGGC AAGATTCGTT GATCCTGCAG 
AGAGCTATCC TGAGAGAGCT GCAGTCGGAC ACCATCAGAG CATCGCTGGC AACTTCGCTC 
AGGGGAGAGA GAGGAGAACC TGGACCTAAA GGAGACCAAG GGCCTCCTGG AGTTAAAGGT 
GATTCAGGAT TTCCCGGGAT TCCAGGTCCT CCTGGTCTTA TTGGTCATCC TGGACAGGAA 
GGCCCTCGAG GACCCAAAGG GAGCGCAGGT GAATCAGGAA GTGATGGTTT ACCTGGACCG 
AGGGGTCGTG AAGGTCCAGC GGGTCCCAGA GGAGAACCAG GACCCCCAGG AATTGGGGAG 
AAAGGAGAGA AAGGCTCATT TGGTGATGTT GGCGCTCCTG GGATAGCAGG ACCACCAGGA 
CCCGTAGGGC CCAAAGGTGA TGCAGGTGCA CAAGGAGTAC CGGGACTTAC AGGAGCACCT 
GGACCCCAAG GCTTCCGCGG AGATTCTGGT GATCCCGGAC CCAAGGGTGA TAAAGGACCT 
GCTGGGCCAC CTGGGGTCAA AGGTGATCAG GGAGAGAGAG GACCACGTGG CGCCACTGGT 
GAGCCGGGTC AGCCTGGACC AGCAGGTCCT CCTGGTGAGA AAGGTCTTAA GGGATCAACA 
GGTGCACCAG GACCAGATGG TGTTAAAGGA CTTAGGGGTG ACCAAGGACC CGTTGGGTTG 
CCTGGTGCTC GAGGACCATC AGGACCTCCC GGAGATTCAG GAATACCAGG AACACCAGGA 
CTCCAAGGAC CACCAGGACT GCCAGGTACA CCAGGCCAAC CAGGAGCTAA AGGTGAGGCC 
GGTGAGCCTG GAAGAGTCAT TTCTGCAGGC GCTAATGCTA ACACGGTGGC TGTATCTGGA 
CCACCAGGAC CTCCTGGAGC TCCAGGTCCT GCTGGTCCAC CTGGACTATC AGGTCCTATT 
GGTCCTGCTG GTCTTCCTGG TTCACCTGGT GCAAAGGGAG ATAAAGGAGA CAAAGGAGAG 
GATGGGAAAC CTGGAGTCAC TGAGAGTGCA GGGACAGAGA AATCAGAAGC AGGGGAACCC 
GGTCCTCCAG GACCACCAGG ACCTCCAGGA CCACCCGGGG ATGATGGAAA GCCAGGTGTT 
GGAGAACCTG GTCCAGCTGG TCCTCCTGGT GAAAAAGGTG AACCTGGAAG CTTTGTACCT 
ACTTCAGAAA CATTCTTTGC TGGACCACCT GGGCCTCCTG GACCTATTGG ACCAAAGGGA 
TCACAAGGTG ATGTTGGCCC TCAAGGTTTC CCTGGTCAAC CTGGATTACC TGGAGAACCT 
GGAATTGGTA TTCCAGGACA GCCGGGATCA CCAGGACCAC GTGGAGAGCA AGGTCTTGAT 
GGTCCACTTG GACCACAGGG ACCTCAAGGG CCAATGGGTC CCATGGGCCC AGAAGGTCCA 
AAAGGTGATG CTGGAGTCCC AGGTGCCCCT GGTATTCCAG GTTATTCTTA CGGTGGAGGA 
TCCAGAAGTG CTCCGGGACC ACCAGGTCCA CCTGGACCCC CAGGGCCTCC TGGAGAACCA 
GGTTCTCAAG GTGACGGGTC ACCGAATGCT GTTCAACAGA TTTCAGACTA CCTCAGAAGT 
GAGCCCATTA GACAATACCT AGCTGGACCT CCAGGCCCGC CTGGACCACC AGGTCCCTCT 
GGATCAGCCT CTGATGATGC TCTGGCTAGC AGAGTCATTG AATATCTTCA ACGAGATGAG 
GTGAGACAGT ACCTGATTGG TCCTCCAGGA CCCCCGGGAC CACCAGGTGT GCCAGGTGGA 
TATGGCTTTA ACACTCAGGA AGTGGCAGGA CGAGTGATGA GCCTTATTAA CGATGCTGGT 
TTTAGAGGTT TACCAGGACC TCCCGGCCCA CCAGGACCTC AAGGACCTCC TGGAAACTCT 
GGAGGACTGG TGTCTTACGC TGCTAACGAA AACCAGCAGC ACATCCGCGC AGAGCCGCAG 
GAGTACATCA AGAGCCATCC AAGGGCTGAT GAGAATTTGG ATTACACAGC CATCGCTAAA 
CAGGTTTCAG ATTACATTAA GGCTCATGGC CTGTTAAATG GAAATGCTGG CTTTCCAGGT 
GCACCAGGTC GTCAAGGATA TCCTGGACCA CCAGGGTTTC CAGGAGAGCC CGGAACACCA 
GGACAGCCTG GACCACCAGG ACAGGCTGGA CCACCAGGAC TGCCTGGACC ACCAGGACTC 
GACACTGTAC ACAATTACTC CAACATGACT GCTGTCTTGG ACTACATCAA AGAAAATCAT 
ATGAAAGGAC AAAAAGGCGA CCTGGGATTT CCAGGAATTC CAGGATCCCC AGGTATTGAT 
GGTAAACAAG GCATAGTAGG CTTACCAGGC ATAAAGGGAC AAAAAGGAGA AAGAGGTGAA 
GCAACATACG TCAACCGAAG GAGGAGGAGG AGCACAGTAA TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160168.1
CDS	181..4425
Translation	MDKLTTTKIVKVEGGSGGSGKEIQKHTETVTTTRLTSLPPKAHSGQQILTSIGNGSGSAGGAVLLEKKFITHSSAEGGASRSYVISSSSSGSSASSSSGAGSVISGFDGISGEISVTGPITSSTVKSSTSAVKSSSVVSTSITSSSAAGGELSSGYGSISSSSSEFVSGSSGGSISTSEGSMSKSRSGGGSSAVMSGEGTSSSKTVSKFSSSSPSGTRKGLSYSTVPTERKTIMNITTGGYEGSSSGNSSPEYIKKEYVSTSSAATRGRTQTRESEIRARLQSASPSARWTELDDVKRLLKGSRSGSISPPRSPTSTLPIPKKASIDVRHESQSGFDGTVLDAGFSGYYTNPNNLSYTTLQQPSSPTGGMQNNLTLSSSALDTTVYGMQNNLSTSGGAMMANGRCTGYVYGVPKNVGSAGLATTISAIPSSPITADDVFMKDGKLIAIGKENVAAKTETERLIMSKNTGKQFVPSSFNAGADTFSEDSLKREKKMISSATDPQTTTITGSKSSTKNKATYADIRKDDSDGAGCGFWTCCSWWKWLLALLFALLLLLGLLCGLIALGEQLRRLKARVDAIDGGGTSPRTSGLKTDDIPGVQSGNTINLGYEPAGQDSLILQRAILRELQSDTIRASLATSLRGERGEPGPKGDQGPPGVKGDSGFPGIPGPPGLIGHPGQEGPRGPKGSAGESGSDGLPGPRGREGPAGPRGEPGPPGIGEKGEKGSFGDVGAPGIAGPPGPVGPKGDAGAQGVPGLTGAPGPQGFRGDSGDPGPKGDKGPAGPPGVKGDQGERGPRGATGEPGQPGPAGPPGEKGLKGSTGAPGPDGVKGLRGDQGPVGLPGARGPSGPPGDSGIPGTPGLQGPPGLPGTPGQPGAKGEAGEPGRVISAGANANTVAVSGPPGPPGAPGPAGPPGLSGPIGPAGLPGSPGAKGDKGDKGEDGKPGVTESAGTEKSEAGEPGPPGPPGPPGPPGDDGKPGVGEPGPAGPPGEKGEPGSFVPTSETFFAGPPGPPGPIGPKGSQGDVGPQGFPGQPGLPGEPGIGIPGQPGSPGPRGEQGLDGPLGPQGPQGPMGPMGPEGPKGDAGVPGAPGIPGYSYGGGSRSAPGPPGPPGPPGPPGEPGSQGDGSPNAVQQISDYLRSEPIRQYLAGPPGPPGPPGPSGSASDDALASRVIEYLQRDEVRQYLIGPPGPPGPPGVPGGYGFNTQEVAGRVMSLINDAGFRGLPGPPGPPGPQGPPGNSGGLVSYAANENQQHIRAEPQEYIKSHPRADENLDYTAIAKQVSDYIKAHGLLNGNAGFPGAPGRQGYPGPPGFPGEPGTPGQPGPPGQAGPPGLPGPPGLDTVHNYSNMTAVLDYIKENHMKGQKGDLGFPGIPGSPGIDGKQGIVGLPGIKGQKGERGEATYVNRRRRRSTVI

Target ORF information:

RefSeq Version	XM_005160111.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type XVII, alpha 1a (col17a1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160111.3

ORF Insert Sequence:

ATGGACAAGC TAACAACAAC CAAGATTGTT AAAGTTGAGG GGGGATCTGG AGGATCTGGA 
AAAGAAATCC AGAAACATAC AGAAACTGTG ACGACTACAA GACTGACATC TCTACCGCCA 
AAAGCCCACA GCGGCCAGCA GATACTGACC TCCATCGGGA ACGGCTCAGG ATCTGCAGGA 
GGAGCTGTTT TGCTGGAGAA GAAGTTCATC ACTCACAGCA GCGCTGAAGG AGGAGCTTCC 
AGGAGTTATG TCATATCATC CTCCTCATCT GGAAGTTCAG CCAGCAGCAG CTCAGGAGCA 
GGATCTGTGA TCAGTGGATT TGACGGTATA TCTGGCGAAA TCAGTGTAAC CGGACCGATC 
ACTAGCTCTA CGGTTAAAAG CTCTACATCT GCAGTTAAAA GCTCTAGCGT GGTCTCTACC 
TCCATCACAT CCTCGTCAGC CGCAGGCGGA GAGCTTTCCT CTGGATACGG CTCCATCTCC 
TCATCCTCCT CCGAGTTCGT GTCTGGTTCC TCAGGAGGAT CCATCAGTAC TTCTGAAGGC 
AGCATGAGTA AATCTAGAAG CGGTGGAGGA TCATCTGCAG TCATGTCTGG CGAAGGAACA 
TCCAGCTCCA AGACTGTGTC CAAATTCAGC TCCTCGTCCC CTAGTGGAAC AAGGAAAGGT 
TTGAGTTACA GCACAGTTCC TACTGAGAGG AAGACCATTA TGAACATTAC CACAGGAGGA 
TATGAGGGGA GCTCCAGTGG CAATTCCTCC CCTGAGTACA TAAAGAAAGA GTACGTTTCC 
ACTAGCAGTG CTGCCACCAG AGGGCGCACT CAAACACGAG AGAGTGAGAT CAGAGCCAGA 
CTGCAGAGCG CCTCGCCCTC CGCTAGATGG ACAGAGTTGG ATGATGTGAA GAGATTACTG 
AAGGGAAGTC GCTCTGGGAG CATCAGTCCA CCACGTTCTC CCACCAGCAC TCTCCCTATC 
CCCAAAAAAG CCAGCATTGA CGTACGCCAC GAGAGCCAGT CAGGCTTTGA TGGCACAGTT 
TTGGATGCTG GATTCAGTGG ATATTACACC AATCCCAACA ATCTCAGTTA CACCACATTG 
CAACAACCAT CATCACCCAC AGGCGGCATG CAAAACAATC TGACCCTCAG TTCATCTGCC 
CTTGATACTA CAGTGTATGG GATGCAGAAT AACCTGTCTA CATCTGGCGG TGCTATGATG 
GCTAATGGAA GGTGCACTGG CTATGTGTAT GGGGTCCCGA AAAATGTTGG CAGCGCTGGA 
TTGGCCACTA CTATTTCTGC TATTCCAAGC AGTCCTATCA CCGCTGATGA CGTGTTTATG 
AAAGATGGCA AGTTAATTGC GATTGGGAAG GAAAATGTAG CGGCTAAGAC AGAGACCGAG 
AGGCTGATCA TGTCCAAAAA CACTGGCAAA CAGTTCGTCC CTTCATCCTT CAATGCTGGA 
GCAGACACGT TTTCTGAAGA TTCACTCAAG AGGGAGAAAA AGATGATCTC GAGCGCCACA 
GATCCACAGA CCACCACCAT TACTGGTTCC AAATCCTCAA CAAAAAACAA AGCAACATAT 
GCAGATATTC GTAAAGATGA TTCCGACGGC GCTGGATGTG GATTCTGGAC GTGTTGCTCG 
TGGTGGAAAT GGCTTCTGGC TTTGTTATTT GCTCTTCTCC TTCTGCTGGG ACTCCTCTGT 
GGACTGATCG CACTAGGTGA GCAGCTGAGG AGGCTGAAGG CTCGTGTGGA TGCTATCGAT 
GGTGGAGGAA CCTCTCCTCG AACCAGCGGT TTGAAAACTG ACGACATTCC TGGGGTCCAA 
TCTGGTAACA CGATAAACCT GGGTTATGAA CCTGCTGGGC AAGATTCGTT GATCCTGCAG 
AGAGCTATCC TGAGAGAGCT GCAGTCGGAC ACCATCAGAG CATCGCTGGC AACTTCGCTC 
AGGGGAGAGA GAGGAGAACC TGGACCTAAA GGAGACCAAG GGCCTCCTGG AGTTAAAGGT 
GATTCAGGAT TTCCCGGGAT TCCAGGTCCT CCTGGTCTTA TTGGTCATCC TGGACAGGAA 
GGCCCTCGAG GACCCAAAGG GAGCGCAGGT GAATCAGGAA GTGATGGTTT ACCTGGACCG 
AGGGGTCGTG AAGGTCCAGC GGGTCCCAGA GGAGAACCAG GACCCCCAGG AATTGGGGAG 
AAAGGAGAGA AAGGCTCATT TGGTGATGTT GGCGCTCCTG GGATAGCAGG ACCACCAGGA 
CCCGTAGGGC CCAAAGGTGA TGCAGGTGCA CAAGGAGTAC CGGGACTTAC AGGAGCACCT 
GGACCCCAAG GCTTCCGCGG AGATTCTGGT GATCCCGGAC CCAAGGGTGA TAAAGGACCT 
GCTGGGCCAC CTGGGGTCAA AGGTGATCAG GGAGAGAGAG GACCACGTGG CGCCACTGGT 
GAGCCGGGTC AGCCTGGACC AGCAGGTCCT CCTGGTGAGA AAGGTCTTAA GGGATCAACA 
GGTGCACCAG GACCAGATGG TGTTAAAGGA CTTAGGGGTG ACCAAGGACC CGTTGGGTTG 
CCTGGTGCTC GAGGACCATC AGGACCTCCC GGAGATTCAG GAATACCAGG AACACCAGGA 
CTCCAAGGAC CACCAGGACT GCCAGGTACA CCAGGCCAAC CAGGAGCTAA AGGTGAGGCC 
GGTGAGCCTG GAAGAGTCAT TTCTGCAGGC GCTAATGCTA ACACGGTGGC TGTATCTGGA 
CCACCAGGAC CTCCTGGAGC TCCAGGTCCT GCTGGTCCAC CTGGACTATC AGGTCCTATT 
GGTCCTGCTG GTCTTCCTGG TTCACCTGGT GCAAAGGGAG ATAAAGGAGA CAAAGGAGAG 
GATGGGAAAC CTGGAGTCAC TGAGAGTGCA GGGACAGAGA AATCAGAAGC AGGGGAACCC 
GGTCCTCCAG GACCACCAGG ACCTCCAGGA CCACCCGGGG ATGATGGAAA GCCAGGTGTT 
GGAGAACCTG GTCCAGCTGG TCCTCCTGGT GAAAAAGGTG AACCTGGAAG CTTTGTACCT 
ACTTCAGAAA CATTCTTTGC TGGACCACCT GGGCCTCCTG GACCTATTGG ACCAAAGGGA 
TCACAAGGTG ATGTTGGCCC TCAAGGTTTC CCTGGTCAAC CTGGATTACC TGGAGAACCT 
GGAATTGGTA TTCCAGGACA GCCGGGATCA CCAGGACCAC GTGGAGAGCA AGGTCTTGAT 
GGTCCACTTG GACCACAGGG ACCTCAAGGG CCAATGGGTC CCATGGGCCC AGAAGGTCCA 
AAAGGTGATG CTGGAGTCCC AGGTGCCCCT GGTATTCCAG GTTATTCTTA CGGTGGAGGA 
TCCAGAAGTG CTCCGGGACC ACCAGGTCCA CCTGGACCCC CAGGGCCTCC TGGAGAACCA 
GGTTCTCAAG GTGACGGGTC ACCGAATGCT GTTCAACAGA TTTCAGACTA CCTCAGAAGT 
GAGCCCATTA GACAATACCT AGCTGGACCT CCAGGCCCGC CTGGACCACC AGGTCCCTCT 
GGATCAGCCT CTGATGATGC TCTGGCTAGC AGAGTCATTG AATATCTTCA ACGAGATGAG 
GTGAGACAGT ACCTGATTGG TCCTCCAGGA CCCCCGGGAC CACCAGGTGT GCCAGGTGGA 
TATGGCTTTA ACACTCAGGA AGTGGCAGGA CGAGTGATGA GCCTTATTAA CGATGCTGGT 
TTTAGAGGTT TACCAGGACC TCCCGGCCCA CCAGGACCTC AAGGACCTCC TGGAAACTCT 
GGAGGACTGG TGTCTTACGC TGCTAACGAA AACCAGCAGC ACATCCGCGC AGAGCCGCAG 
GAGTACATCA AGAGCCATCC AAGGGCTGAT GAGAATTTGG ATTACACAGC CATCGCTAAA 
CAGGTTTCAG ATTACATTAA GGCTCATGGC CTGTTAAATG GAAATGCTGG CTTTCCAGGT 
GCACCAGGTC GTCAAGGATA TCCTGGACCA CCAGGGTTTC CAGGAGAGCC CGGAACACCA 
GGACAGCCTG GACCACCAGG ACAGGCTGGA CCACCAGGAC TGCCTGGACC ACCAGGACTC 
GACACTGTAC ACAATTACTC CAACATGACT GCTGTCTTGG ACTACATCAA AGAAAATCAT 
ATGAAAGGAC AAAAAGGCGA CCTGGGATTT CCAGGAATTC CAGGATCCCC AGGTATTGAT 
GGTAAACAAG GCATAGTAGG CTTACCAGGC ATAAAGGGAC AAAAAGGAGA AAGAGGTGAA 
GCAACATACG TCAACCGAAG GAGGAGGAGG AGCACAGTAA TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col17a1a ( XM_005160112.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160169.1 Danio rerio collagen, type XVII, alpha 1a (col17a1a), transcript variant X2, mRNA.

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CloneID	ODa06151
Clone ID Related Accession (Same CDS sequence)	XM_005160111.4 , XM_005160111.3 , XM_005160112.3 , XM_005160112.4
Accession Version	XM_005160112.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	collagen, type XVII, alpha 1a isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160112.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAAGC TAACAACAAC CAAGATTGTT AAAGTTGAGG GGGGATCTGG AGGATCTGGA 
AAAGAAATCC AGAAACATAC AGAAACTGTG ACGACTACAA GACTGACATC TCTACCGCCA 
AAAGCCCACA GCGGCCAGCA GATACTGACC TCCATCGGGA ACGGCTCAGG ATCTGCAGGA 
GGAGCTGTTT TGCTGGAGAA GAAGTTCATC ACTCACAGCA GCGCTGAAGG AGGAGCTTCC 
AGGAGTTATG TCATATCATC CTCCTCATCT GGAAGTTCAG CCAGCAGCAG CTCAGGAGCA 
GGATCTGTGA TCAGTGGATT TGACGGTATA TCTGGCGAAA TCAGTGTAAC CGGACCGATC 
ACTAGCTCTA CGGTTAAAAG CTCTACATCT GCAGTTAAAA GCTCTAGCGT GGTCTCTACC 
TCCATCACAT CCTCGTCAGC CGCAGGCGGA GAGCTTTCCT CTGGATACGG CTCCATCTCC 
TCATCCTCCT CCGAGTTCGT GTCTGGTTCC TCAGGAGGAT CCATCAGTAC TTCTGAAGGC 
AGCATGAGTA AATCTAGAAG CGGTGGAGGA TCATCTGCAG TCATGTCTGG CGAAGGAACA 
TCCAGCTCCA AGACTGTGTC CAAATTCAGC TCCTCGTCCC CTAGTGGAAC AAGGAAAGGT 
TTGAGTTACA GCACAGTTCC TACTGAGAGG AAGACCATTA TGAACATTAC CACAGGAGGA 
TATGAGGGGA GCTCCAGTGG CAATTCCTCC CCTGAGTACA TAAAGAAAGA GTACGTTTCC 
ACTAGCAGTG CTGCCACCAG AGGGCGCACT CAAACACGAG AGAGTGAGAT CAGAGCCAGA 
CTGCAGAGCG CCTCGCCCTC CGCTAGATGG ACAGAGTTGG ATGATGTGAA GAGATTACTG 
AAGGGAAGTC GCTCTGGGAG CATCAGTCCA CCACGTTCTC CCACCAGCAC TCTCCCTATC 
CCCAAAAAAG CCAGCATTGA CGTACGCCAC GAGAGCCAGT CAGGCTTTGA TGGCACAGTT 
TTGGATGCTG GATTCAGTGG ATATTACACC AATCCCAACA ATCTCAGTTA CACCACATTG 
CAACAACCAT CATCACCCAC AGGCGGCATG CAAAACAATC TGACCCTCAG TTCATCTGCC 
CTTGATACTA CAGTGTATGG GATGCAGAAT AACCTGTCTA CATCTGGCGG TGCTATGATG 
GCTAATGGAA GGTGCACTGG CTATGTGTAT GGGGTCCCGA AAAATGTTGG CAGCGCTGGA 
TTGGCCACTA CTATTTCTGC TATTCCAAGC AGTCCTATCA CCGCTGATGA CGTGTTTATG 
AAAGATGGCA AGTTAATTGC GATTGGGAAG GAAAATGTAG CGGCTAAGAC AGAGACCGAG 
AGGCTGATCA TGTCCAAAAA CACTGGCAAA CAGTTCGTCC CTTCATCCTT CAATGCTGGA 
GCAGACACGT TTTCTGAAGA TTCACTCAAG AGGGAGAAAA AGATGATCTC GAGCGCCACA 
GATCCACAGA CCACCACCAT TACTGGTTCC AAATCCTCAA CAAAAAACAA AGCAACATAT 
GCAGATATTC GTAAAGATGA TTCCGACGGC GCTGGATGTG GATTCTGGAC GTGTTGCTCG 
TGGTGGAAAT GGCTTCTGGC TTTGTTATTT GCTCTTCTCC TTCTGCTGGG ACTCCTCTGT 
GGACTGATCG CACTAGGTGA GCAGCTGAGG AGGCTGAAGG CTCGTGTGGA TGCTATCGAT 
GGTGGAGGAA CCTCTCCTCG AACCAGCGGT TTGAAAACTG ACGACATTCC TGGGGTCCAA 
TCTGGTAACA CGATAAACCT GGGTTATGAA CCTGCTGGGC AAGATTCGTT GATCCTGCAG 
AGAGCTATCC TGAGAGAGCT GCAGTCGGAC ACCATCAGAG CATCGCTGGC AACTTCGCTC 
AGGGGAGAGA GAGGAGAACC TGGACCTAAA GGAGACCAAG GGCCTCCTGG AGTTAAAGGT 
GATTCAGGAT TTCCCGGGAT TCCAGGTCCT CCTGGTCTTA TTGGTCATCC TGGACAGGAA 
GGCCCTCGAG GACCCAAAGG GAGCGCAGGT GAATCAGGAA GTGATGGTTT ACCTGGACCG 
AGGGGTCGTG AAGGTCCAGC GGGTCCCAGA GGAGAACCAG GACCCCCAGG AATTGGGGAG 
AAAGGAGAGA AAGGCTCATT TGGTGATGTT GGCGCTCCTG GGATAGCAGG ACCACCAGGA 
CCCGTAGGGC CCAAAGGTGA TGCAGGTGCA CAAGGAGTAC CGGGACTTAC AGGAGCACCT 
GGACCCCAAG GCTTCCGCGG AGATTCTGGT GATCCCGGAC CCAAGGGTGA TAAAGGACCT 
GCTGGGCCAC CTGGGGTCAA AGGTGATCAG GGAGAGAGAG GACCACGTGG CGCCACTGGT 
GAGCCGGGTC AGCCTGGACC AGCAGGTCCT CCTGGTGAGA AAGGTCTTAA GGGATCAACA 
GGTGCACCAG GACCAGATGG TGTTAAAGGA CTTAGGGGTG ACCAAGGACC CGTTGGGTTG 
CCTGGTGCTC GAGGACCATC AGGACCTCCC GGAGATTCAG GAATACCAGG AACACCAGGA 
CTCCAAGGAC CACCAGGACT GCCAGGTACA CCAGGCCAAC CAGGAGCTAA AGGTGAGGCC 
GGTGAGCCTG GAAGAGTCAT TTCTGCAGGC GCTAATGCTA ACACGGTGGC TGTATCTGGA 
CCACCAGGAC CTCCTGGAGC TCCAGGTCCT GCTGGTCCAC CTGGACTATC AGGTCCTATT 
GGTCCTGCTG GTCTTCCTGG TTCACCTGGT GCAAAGGGAG ATAAAGGAGA CAAAGGAGAG 
GATGGGAAAC CTGGAGTCAC TGAGAGTGCA GGGACAGAGA AATCAGAAGC AGGGGAACCC 
GGTCCTCCAG GACCACCAGG ACCTCCAGGA CCACCCGGGG ATGATGGAAA GCCAGGTGTT 
GGAGAACCTG GTCCAGCTGG TCCTCCTGGT GAAAAAGGTG AACCTGGAAG CTTTGTACCT 
ACTTCAGAAA CATTCTTTGC TGGACCACCT GGGCCTCCTG GACCTATTGG ACCAAAGGGA 
TCACAAGGTG ATGTTGGCCC TCAAGGTTTC CCTGGTCAAC CTGGATTACC TGGAGAACCT 
GGAATTGGTA TTCCAGGACA GCCGGGATCA CCAGGACCAC GTGGAGAGCA AGGTCTTGAT 
GGTCCACTTG GACCACAGGG ACCTCAAGGG CCAATGGGTC CCATGGGCCC AGAAGGTCCA 
AAAGGTGATG CTGGAGTCCC AGGTGCCCCT GGTATTCCAG GTTATTCTTA CGGTGGAGGA 
TCCAGAAGTG CTCCGGGACC ACCAGGTCCA CCTGGACCCC CAGGGCCTCC TGGAGAACCA 
GGTTCTCAAG GTGACGGGTC ACCGAATGCT GTTCAACAGA TTTCAGACTA CCTCAGAAGT 
GAGCCCATTA GACAATACCT AGCTGGACCT CCAGGCCCGC CTGGACCACC AGGTCCCTCT 
GGATCAGCCT CTGATGATGC TCTGGCTAGC AGAGTCATTG AATATCTTCA ACGAGATGAG 
GTGAGACAGT ACCTGATTGG TCCTCCAGGA CCCCCGGGAC CACCAGGTGT GCCAGGTGGA 
TATGGCTTTA ACACTCAGGA AGTGGCAGGA CGAGTGATGA GCCTTATTAA CGATGCTGGT 
TTTAGAGGTT TACCAGGACC TCCCGGCCCA CCAGGACCTC AAGGACCTCC TGGAAACTCT 
GGAGGACTGG TGTCTTACGC TGCTAACGAA AACCAGCAGC ACATCCGCGC AGAGCCGCAG 
GAGTACATCA AGAGCCATCC AAGGGCTGAT GAGAATTTGG ATTACACAGC CATCGCTAAA 
CAGGTTTCAG ATTACATTAA GGCTCATGGC CTGTTAAATG GAAATGCTGG CTTTCCAGGT 
GCACCAGGTC GTCAAGGATA TCCTGGACCA CCAGGGTTTC CAGGAGAGCC CGGAACACCA 
GGACAGCCTG GACCACCAGG ACAGGCTGGA CCACCAGGAC TGCCTGGACC ACCAGGACTC 
GACACTGTAC ACAATTACTC CAACATGACT GCTGTCTTGG ACTACATCAA AGAAAATCAT 
ATGAAAGGAC AAAAAGGCGA CCTGGGATTT CCAGGAATTC CAGGATCCCC AGGTATTGAT 
GGTAAACAAG GCATAGTAGG CTTACCAGGC ATAAAGGGAC AAAAAGGAGA AAGAGGTGAA 
GCAACATACG TCAACCGAAG GAGGAGGAGG AGCACAGTAA TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160169.1
CDS	140..4384
Translation	MDKLTTTKIVKVEGGSGGSGKEIQKHTETVTTTRLTSLPPKAHSGQQILTSIGNGSGSAGGAVLLEKKFITHSSAEGGASRSYVISSSSSGSSASSSSGAGSVISGFDGISGEISVTGPITSSTVKSSTSAVKSSSVVSTSITSSSAAGGELSSGYGSISSSSSEFVSGSSGGSISTSEGSMSKSRSGGGSSAVMSGEGTSSSKTVSKFSSSSPSGTRKGLSYSTVPTERKTIMNITTGGYEGSSSGNSSPEYIKKEYVSTSSAATRGRTQTRESEIRARLQSASPSARWTELDDVKRLLKGSRSGSISPPRSPTSTLPIPKKASIDVRHESQSGFDGTVLDAGFSGYYTNPNNLSYTTLQQPSSPTGGMQNNLTLSSSALDTTVYGMQNNLSTSGGAMMANGRCTGYVYGVPKNVGSAGLATTISAIPSSPITADDVFMKDGKLIAIGKENVAAKTETERLIMSKNTGKQFVPSSFNAGADTFSEDSLKREKKMISSATDPQTTTITGSKSSTKNKATYADIRKDDSDGAGCGFWTCCSWWKWLLALLFALLLLLGLLCGLIALGEQLRRLKARVDAIDGGGTSPRTSGLKTDDIPGVQSGNTINLGYEPAGQDSLILQRAILRELQSDTIRASLATSLRGERGEPGPKGDQGPPGVKGDSGFPGIPGPPGLIGHPGQEGPRGPKGSAGESGSDGLPGPRGREGPAGPRGEPGPPGIGEKGEKGSFGDVGAPGIAGPPGPVGPKGDAGAQGVPGLTGAPGPQGFRGDSGDPGPKGDKGPAGPPGVKGDQGERGPRGATGEPGQPGPAGPPGEKGLKGSTGAPGPDGVKGLRGDQGPVGLPGARGPSGPPGDSGIPGTPGLQGPPGLPGTPGQPGAKGEAGEPGRVISAGANANTVAVSGPPGPPGAPGPAGPPGLSGPIGPAGLPGSPGAKGDKGDKGEDGKPGVTESAGTEKSEAGEPGPPGPPGPPGPPGDDGKPGVGEPGPAGPPGEKGEPGSFVPTSETFFAGPPGPPGPIGPKGSQGDVGPQGFPGQPGLPGEPGIGIPGQPGSPGPRGEQGLDGPLGPQGPQGPMGPMGPEGPKGDAGVPGAPGIPGYSYGGGSRSAPGPPGPPGPPGPPGEPGSQGDGSPNAVQQISDYLRSEPIRQYLAGPPGPPGPPGPSGSASDDALASRVIEYLQRDEVRQYLIGPPGPPGPPGVPGGYGFNTQEVAGRVMSLINDAGFRGLPGPPGPPGPQGPPGNSGGLVSYAANENQQHIRAEPQEYIKSHPRADENLDYTAIAKQVSDYIKAHGLLNGNAGFPGAPGRQGYPGPPGFPGEPGTPGQPGPPGQAGPPGLPGPPGLDTVHNYSNMTAVLDYIKENHMKGQKGDLGFPGIPGSPGIDGKQGIVGLPGIKGQKGERGEATYVNRRRRRSTVI

Target ORF information:

RefSeq Version	XM_005160112.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type XVII, alpha 1a (col17a1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160112.3

ORF Insert Sequence:

ATGGACAAGC TAACAACAAC CAAGATTGTT AAAGTTGAGG GGGGATCTGG AGGATCTGGA 
AAAGAAATCC AGAAACATAC AGAAACTGTG ACGACTACAA GACTGACATC TCTACCGCCA 
AAAGCCCACA GCGGCCAGCA GATACTGACC TCCATCGGGA ACGGCTCAGG ATCTGCAGGA 
GGAGCTGTTT TGCTGGAGAA GAAGTTCATC ACTCACAGCA GCGCTGAAGG AGGAGCTTCC 
AGGAGTTATG TCATATCATC CTCCTCATCT GGAAGTTCAG CCAGCAGCAG CTCAGGAGCA 
GGATCTGTGA TCAGTGGATT TGACGGTATA TCTGGCGAAA TCAGTGTAAC CGGACCGATC 
ACTAGCTCTA CGGTTAAAAG CTCTACATCT GCAGTTAAAA GCTCTAGCGT GGTCTCTACC 
TCCATCACAT CCTCGTCAGC CGCAGGCGGA GAGCTTTCCT CTGGATACGG CTCCATCTCC 
TCATCCTCCT CCGAGTTCGT GTCTGGTTCC TCAGGAGGAT CCATCAGTAC TTCTGAAGGC 
AGCATGAGTA AATCTAGAAG CGGTGGAGGA TCATCTGCAG TCATGTCTGG CGAAGGAACA 
TCCAGCTCCA AGACTGTGTC CAAATTCAGC TCCTCGTCCC CTAGTGGAAC AAGGAAAGGT 
TTGAGTTACA GCACAGTTCC TACTGAGAGG AAGACCATTA TGAACATTAC CACAGGAGGA 
TATGAGGGGA GCTCCAGTGG CAATTCCTCC CCTGAGTACA TAAAGAAAGA GTACGTTTCC 
ACTAGCAGTG CTGCCACCAG AGGGCGCACT CAAACACGAG AGAGTGAGAT CAGAGCCAGA 
CTGCAGAGCG CCTCGCCCTC CGCTAGATGG ACAGAGTTGG ATGATGTGAA GAGATTACTG 
AAGGGAAGTC GCTCTGGGAG CATCAGTCCA CCACGTTCTC CCACCAGCAC TCTCCCTATC 
CCCAAAAAAG CCAGCATTGA CGTACGCCAC GAGAGCCAGT CAGGCTTTGA TGGCACAGTT 
TTGGATGCTG GATTCAGTGG ATATTACACC AATCCCAACA ATCTCAGTTA CACCACATTG 
CAACAACCAT CATCACCCAC AGGCGGCATG CAAAACAATC TGACCCTCAG TTCATCTGCC 
CTTGATACTA CAGTGTATGG GATGCAGAAT AACCTGTCTA CATCTGGCGG TGCTATGATG 
GCTAATGGAA GGTGCACTGG CTATGTGTAT GGGGTCCCGA AAAATGTTGG CAGCGCTGGA 
TTGGCCACTA CTATTTCTGC TATTCCAAGC AGTCCTATCA CCGCTGATGA CGTGTTTATG 
AAAGATGGCA AGTTAATTGC GATTGGGAAG GAAAATGTAG CGGCTAAGAC AGAGACCGAG 
AGGCTGATCA TGTCCAAAAA CACTGGCAAA CAGTTCGTCC CTTCATCCTT CAATGCTGGA 
GCAGACACGT TTTCTGAAGA TTCACTCAAG AGGGAGAAAA AGATGATCTC GAGCGCCACA 
GATCCACAGA CCACCACCAT TACTGGTTCC AAATCCTCAA CAAAAAACAA AGCAACATAT 
GCAGATATTC GTAAAGATGA TTCCGACGGC GCTGGATGTG GATTCTGGAC GTGTTGCTCG 
TGGTGGAAAT GGCTTCTGGC TTTGTTATTT GCTCTTCTCC TTCTGCTGGG ACTCCTCTGT 
GGACTGATCG CACTAGGTGA GCAGCTGAGG AGGCTGAAGG CTCGTGTGGA TGCTATCGAT 
GGTGGAGGAA CCTCTCCTCG AACCAGCGGT TTGAAAACTG ACGACATTCC TGGGGTCCAA 
TCTGGTAACA CGATAAACCT GGGTTATGAA CCTGCTGGGC AAGATTCGTT GATCCTGCAG 
AGAGCTATCC TGAGAGAGCT GCAGTCGGAC ACCATCAGAG CATCGCTGGC AACTTCGCTC 
AGGGGAGAGA GAGGAGAACC TGGACCTAAA GGAGACCAAG GGCCTCCTGG AGTTAAAGGT 
GATTCAGGAT TTCCCGGGAT TCCAGGTCCT CCTGGTCTTA TTGGTCATCC TGGACAGGAA 
GGCCCTCGAG GACCCAAAGG GAGCGCAGGT GAATCAGGAA GTGATGGTTT ACCTGGACCG 
AGGGGTCGTG AAGGTCCAGC GGGTCCCAGA GGAGAACCAG GACCCCCAGG AATTGGGGAG 
AAAGGAGAGA AAGGCTCATT TGGTGATGTT GGCGCTCCTG GGATAGCAGG ACCACCAGGA 
CCCGTAGGGC CCAAAGGTGA TGCAGGTGCA CAAGGAGTAC CGGGACTTAC AGGAGCACCT 
GGACCCCAAG GCTTCCGCGG AGATTCTGGT GATCCCGGAC CCAAGGGTGA TAAAGGACCT 
GCTGGGCCAC CTGGGGTCAA AGGTGATCAG GGAGAGAGAG GACCACGTGG CGCCACTGGT 
GAGCCGGGTC AGCCTGGACC AGCAGGTCCT CCTGGTGAGA AAGGTCTTAA GGGATCAACA 
GGTGCACCAG GACCAGATGG TGTTAAAGGA CTTAGGGGTG ACCAAGGACC CGTTGGGTTG 
CCTGGTGCTC GAGGACCATC AGGACCTCCC GGAGATTCAG GAATACCAGG AACACCAGGA 
CTCCAAGGAC CACCAGGACT GCCAGGTACA CCAGGCCAAC CAGGAGCTAA AGGTGAGGCC 
GGTGAGCCTG GAAGAGTCAT TTCTGCAGGC GCTAATGCTA ACACGGTGGC TGTATCTGGA 
CCACCAGGAC CTCCTGGAGC TCCAGGTCCT GCTGGTCCAC CTGGACTATC AGGTCCTATT 
GGTCCTGCTG GTCTTCCTGG TTCACCTGGT GCAAAGGGAG ATAAAGGAGA CAAAGGAGAG 
GATGGGAAAC CTGGAGTCAC TGAGAGTGCA GGGACAGAGA AATCAGAAGC AGGGGAACCC 
GGTCCTCCAG GACCACCAGG ACCTCCAGGA CCACCCGGGG ATGATGGAAA GCCAGGTGTT 
GGAGAACCTG GTCCAGCTGG TCCTCCTGGT GAAAAAGGTG AACCTGGAAG CTTTGTACCT 
ACTTCAGAAA CATTCTTTGC TGGACCACCT GGGCCTCCTG GACCTATTGG ACCAAAGGGA 
TCACAAGGTG ATGTTGGCCC TCAAGGTTTC CCTGGTCAAC CTGGATTACC TGGAGAACCT 
GGAATTGGTA TTCCAGGACA GCCGGGATCA CCAGGACCAC GTGGAGAGCA AGGTCTTGAT 
GGTCCACTTG GACCACAGGG ACCTCAAGGG CCAATGGGTC CCATGGGCCC AGAAGGTCCA 
AAAGGTGATG CTGGAGTCCC AGGTGCCCCT GGTATTCCAG GTTATTCTTA CGGTGGAGGA 
TCCAGAAGTG CTCCGGGACC ACCAGGTCCA CCTGGACCCC CAGGGCCTCC TGGAGAACCA 
GGTTCTCAAG GTGACGGGTC ACCGAATGCT GTTCAACAGA TTTCAGACTA CCTCAGAAGT 
GAGCCCATTA GACAATACCT AGCTGGACCT CCAGGCCCGC CTGGACCACC AGGTCCCTCT 
GGATCAGCCT CTGATGATGC TCTGGCTAGC AGAGTCATTG AATATCTTCA ACGAGATGAG 
GTGAGACAGT ACCTGATTGG TCCTCCAGGA CCCCCGGGAC CACCAGGTGT GCCAGGTGGA 
TATGGCTTTA ACACTCAGGA AGTGGCAGGA CGAGTGATGA GCCTTATTAA CGATGCTGGT 
TTTAGAGGTT TACCAGGACC TCCCGGCCCA CCAGGACCTC AAGGACCTCC TGGAAACTCT 
GGAGGACTGG TGTCTTACGC TGCTAACGAA AACCAGCAGC ACATCCGCGC AGAGCCGCAG 
GAGTACATCA AGAGCCATCC AAGGGCTGAT GAGAATTTGG ATTACACAGC CATCGCTAAA 
CAGGTTTCAG ATTACATTAA GGCTCATGGC CTGTTAAATG GAAATGCTGG CTTTCCAGGT 
GCACCAGGTC GTCAAGGATA TCCTGGACCA CCAGGGTTTC CAGGAGAGCC CGGAACACCA 
GGACAGCCTG GACCACCAGG ACAGGCTGGA CCACCAGGAC TGCCTGGACC ACCAGGACTC 
GACACTGTAC ACAATTACTC CAACATGACT GCTGTCTTGG ACTACATCAA AGAAAATCAT 
ATGAAAGGAC AAAAAGGCGA CCTGGGATTT CCAGGAATTC CAGGATCCCC AGGTATTGAT 
GGTAAACAAG GCATAGTAGG CTTACCAGGC ATAAAGGGAC AAAAAGGAGA AAGAGGTGAA 
GCAACATACG TCAACCGAAG GAGGAGGAGG AGCACAGTAA TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col17a1a ( XM_005160112.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160169.1 Danio rerio collagen, type XVII, alpha 1a (col17a1a), transcript variant X2, mRNA.

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CloneID	ODa06151
Clone ID Related Accession (Same CDS sequence)	XM_005160111.4 , XM_005160111.3 , XM_005160112.3 , XM_005160112.4
Accession Version	XM_005160112.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen, type XVII, alpha 1a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160112.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAAGC TAACAACAAC CAAGATTGTT AAAGTTGAGG GGGGATCTGG AGGATCTGGA 
AAAGAAATCC AGAAACATAC AGAAACTGTG ACGACTACAA GACTGACATC TCTACCGCCA 
AAAGCCCACA GCGGCCAGCA GATACTGACC TCCATCGGGA ACGGCTCAGG ATCTGCAGGA 
GGAGCTGTTT TGCTGGAGAA GAAGTTCATC ACTCACAGCA GCGCTGAAGG AGGAGCTTCC 
AGGAGTTATG TCATATCATC CTCCTCATCT GGAAGTTCAG CCAGCAGCAG CTCAGGAGCA 
GGATCTGTGA TCAGTGGATT TGACGGTATA TCTGGCGAAA TCAGTGTAAC CGGACCGATC 
ACTAGCTCTA CGGTTAAAAG CTCTACATCT GCAGTTAAAA GCTCTAGCGT GGTCTCTACC 
TCCATCACAT CCTCGTCAGC CGCAGGCGGA GAGCTTTCCT CTGGATACGG CTCCATCTCC 
TCATCCTCCT CCGAGTTCGT GTCTGGTTCC TCAGGAGGAT CCATCAGTAC TTCTGAAGGC 
AGCATGAGTA AATCTAGAAG CGGTGGAGGA TCATCTGCAG TCATGTCTGG CGAAGGAACA 
TCCAGCTCCA AGACTGTGTC CAAATTCAGC TCCTCGTCCC CTAGTGGAAC AAGGAAAGGT 
TTGAGTTACA GCACAGTTCC TACTGAGAGG AAGACCATTA TGAACATTAC CACAGGAGGA 
TATGAGGGGA GCTCCAGTGG CAATTCCTCC CCTGAGTACA TAAAGAAAGA GTACGTTTCC 
ACTAGCAGTG CTGCCACCAG AGGGCGCACT CAAACACGAG AGAGTGAGAT CAGAGCCAGA 
CTGCAGAGCG CCTCGCCCTC CGCTAGATGG ACAGAGTTGG ATGATGTGAA GAGATTACTG 
AAGGGAAGTC GCTCTGGGAG CATCAGTCCA CCACGTTCTC CCACCAGCAC TCTCCCTATC 
CCCAAAAAAG CCAGCATTGA CGTACGCCAC GAGAGCCAGT CAGGCTTTGA TGGCACAGTT 
TTGGATGCTG GATTCAGTGG ATATTACACC AATCCCAACA ATCTCAGTTA CACCACATTG 
CAACAACCAT CATCACCCAC AGGCGGCATG CAAAACAATC TGACCCTCAG TTCATCTGCC 
CTTGATACTA CAGTGTATGG GATGCAGAAT AACCTGTCTA CATCTGGCGG TGCTATGATG 
GCTAATGGAA GGTGCACTGG CTATGTGTAT GGGGTCCCGA AAAATGTTGG CAGCGCTGGA 
TTGGCCACTA CTATTTCTGC TATTCCAAGC AGTCCTATCA CCGCTGATGA CGTGTTTATG 
AAAGATGGCA AGTTAATTGC GATTGGGAAG GAAAATGTAG CGGCTAAGAC AGAGACCGAG 
AGGCTGATCA TGTCCAAAAA CACTGGCAAA CAGTTCGTCC CTTCATCCTT CAATGCTGGA 
GCAGACACGT TTTCTGAAGA TTCACTCAAG AGGGAGAAAA AGATGATCTC GAGCGCCACA 
GATCCACAGA CCACCACCAT TACTGGTTCC AAATCCTCAA CAAAAAACAA AGCAACATAT 
GCAGATATTC GTAAAGATGA TTCCGACGGC GCTGGATGTG GATTCTGGAC GTGTTGCTCG 
TGGTGGAAAT GGCTTCTGGC TTTGTTATTT GCTCTTCTCC TTCTGCTGGG ACTCCTCTGT 
GGACTGATCG CACTAGGTGA GCAGCTGAGG AGGCTGAAGG CTCGTGTGGA TGCTATCGAT 
GGTGGAGGAA CCTCTCCTCG AACCAGCGGT TTGAAAACTG ACGACATTCC TGGGGTCCAA 
TCTGGTAACA CGATAAACCT GGGTTATGAA CCTGCTGGGC AAGATTCGTT GATCCTGCAG 
AGAGCTATCC TGAGAGAGCT GCAGTCGGAC ACCATCAGAG CATCGCTGGC AACTTCGCTC 
AGGGGAGAGA GAGGAGAACC TGGACCTAAA GGAGACCAAG GGCCTCCTGG AGTTAAAGGT 
GATTCAGGAT TTCCCGGGAT TCCAGGTCCT CCTGGTCTTA TTGGTCATCC TGGACAGGAA 
GGCCCTCGAG GACCCAAAGG GAGCGCAGGT GAATCAGGAA GTGATGGTTT ACCTGGACCG 
AGGGGTCGTG AAGGTCCAGC GGGTCCCAGA GGAGAACCAG GACCCCCAGG AATTGGGGAG 
AAAGGAGAGA AAGGCTCATT TGGTGATGTT GGCGCTCCTG GGATAGCAGG ACCACCAGGA 
CCCGTAGGGC CCAAAGGTGA TGCAGGTGCA CAAGGAGTAC CGGGACTTAC AGGAGCACCT 
GGACCCCAAG GCTTCCGCGG AGATTCTGGT GATCCCGGAC CCAAGGGTGA TAAAGGACCT 
GCTGGGCCAC CTGGGGTCAA AGGTGATCAG GGAGAGAGAG GACCACGTGG CGCCACTGGT 
GAGCCGGGTC AGCCTGGACC AGCAGGTCCT CCTGGTGAGA AAGGTCTTAA GGGATCAACA 
GGTGCACCAG GACCAGATGG TGTTAAAGGA CTTAGGGGTG ACCAAGGACC CGTTGGGTTG 
CCTGGTGCTC GAGGACCATC AGGACCTCCC GGAGATTCAG GAATACCAGG AACACCAGGA 
CTCCAAGGAC CACCAGGACT GCCAGGTACA CCAGGCCAAC CAGGAGCTAA AGGTGAGGCC 
GGTGAGCCTG GAAGAGTCAT TTCTGCAGGC GCTAATGCTA ACACGGTGGC TGTATCTGGA 
CCACCAGGAC CTCCTGGAGC TCCAGGTCCT GCTGGTCCAC CTGGACTATC AGGTCCTATT 
GGTCCTGCTG GTCTTCCTGG TTCACCTGGT GCAAAGGGAG ATAAAGGAGA CAAAGGAGAG 
GATGGGAAAC CTGGAGTCAC TGAGAGTGCA GGGACAGAGA AATCAGAAGC AGGGGAACCC 
GGTCCTCCAG GACCACCAGG ACCTCCAGGA CCACCCGGGG ATGATGGAAA GCCAGGTGTT 
GGAGAACCTG GTCCAGCTGG TCCTCCTGGT GAAAAAGGTG AACCTGGAAG CTTTGTACCT 
ACTTCAGAAA CATTCTTTGC TGGACCACCT GGGCCTCCTG GACCTATTGG ACCAAAGGGA 
TCACAAGGTG ATGTTGGCCC TCAAGGTTTC CCTGGTCAAC CTGGATTACC TGGAGAACCT 
GGAATTGGTA TTCCAGGACA GCCGGGATCA CCAGGACCAC GTGGAGAGCA AGGTCTTGAT 
GGTCCACTTG GACCACAGGG ACCTCAAGGG CCAATGGGTC CCATGGGCCC AGAAGGTCCA 
AAAGGTGATG CTGGAGTCCC AGGTGCCCCT GGTATTCCAG GTTATTCTTA CGGTGGAGGA 
TCCAGAAGTG CTCCGGGACC ACCAGGTCCA CCTGGACCCC CAGGGCCTCC TGGAGAACCA 
GGTTCTCAAG GTGACGGGTC ACCGAATGCT GTTCAACAGA TTTCAGACTA CCTCAGAAGT 
GAGCCCATTA GACAATACCT AGCTGGACCT CCAGGCCCGC CTGGACCACC AGGTCCCTCT 
GGATCAGCCT CTGATGATGC TCTGGCTAGC AGAGTCATTG AATATCTTCA ACGAGATGAG 
GTGAGACAGT ACCTGATTGG TCCTCCAGGA CCCCCGGGAC CACCAGGTGT GCCAGGTGGA 
TATGGCTTTA ACACTCAGGA AGTGGCAGGA CGAGTGATGA GCCTTATTAA CGATGCTGGT 
TTTAGAGGTT TACCAGGACC TCCCGGCCCA CCAGGACCTC AAGGACCTCC TGGAAACTCT 
GGAGGACTGG TGTCTTACGC TGCTAACGAA AACCAGCAGC ACATCCGCGC AGAGCCGCAG 
GAGTACATCA AGAGCCATCC AAGGGCTGAT GAGAATTTGG ATTACACAGC CATCGCTAAA 
CAGGTTTCAG ATTACATTAA GGCTCATGGC CTGTTAAATG GAAATGCTGG CTTTCCAGGT 
GCACCAGGTC GTCAAGGATA TCCTGGACCA CCAGGGTTTC CAGGAGAGCC CGGAACACCA 
GGACAGCCTG GACCACCAGG ACAGGCTGGA CCACCAGGAC TGCCTGGACC ACCAGGACTC 
GACACTGTAC ACAATTACTC CAACATGACT GCTGTCTTGG ACTACATCAA AGAAAATCAT 
ATGAAAGGAC AAAAAGGCGA CCTGGGATTT CCAGGAATTC CAGGATCCCC AGGTATTGAT 
GGTAAACAAG GCATAGTAGG CTTACCAGGC ATAAAGGGAC AAAAAGGAGA AAGAGGTGAA 
GCAACATACG TCAACCGAAG GAGGAGGAGG AGCACAGTAA TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160169.1
CDS	157..4401
Translation	MDKLTTTKIVKVEGGSGGSGKEIQKHTETVTTTRLTSLPPKAHSGQQILTSIGNGSGSAGGAVLLEKKFITHSSAEGGASRSYVISSSSSGSSASSSSGAGSVISGFDGISGEISVTGPITSSTVKSSTSAVKSSSVVSTSITSSSAAGGELSSGYGSISSSSSEFVSGSSGGSISTSEGSMSKSRSGGGSSAVMSGEGTSSSKTVSKFSSSSPSGTRKGLSYSTVPTERKTIMNITTGGYEGSSSGNSSPEYIKKEYVSTSSAATRGRTQTRESEIRARLQSASPSARWTELDDVKRLLKGSRSGSISPPRSPTSTLPIPKKASIDVRHESQSGFDGTVLDAGFSGYYTNPNNLSYTTLQQPSSPTGGMQNNLTLSSSALDTTVYGMQNNLSTSGGAMMANGRCTGYVYGVPKNVGSAGLATTISAIPSSPITADDVFMKDGKLIAIGKENVAAKTETERLIMSKNTGKQFVPSSFNAGADTFSEDSLKREKKMISSATDPQTTTITGSKSSTKNKATYADIRKDDSDGAGCGFWTCCSWWKWLLALLFALLLLLGLLCGLIALGEQLRRLKARVDAIDGGGTSPRTSGLKTDDIPGVQSGNTINLGYEPAGQDSLILQRAILRELQSDTIRASLATSLRGERGEPGPKGDQGPPGVKGDSGFPGIPGPPGLIGHPGQEGPRGPKGSAGESGSDGLPGPRGREGPAGPRGEPGPPGIGEKGEKGSFGDVGAPGIAGPPGPVGPKGDAGAQGVPGLTGAPGPQGFRGDSGDPGPKGDKGPAGPPGVKGDQGERGPRGATGEPGQPGPAGPPGEKGLKGSTGAPGPDGVKGLRGDQGPVGLPGARGPSGPPGDSGIPGTPGLQGPPGLPGTPGQPGAKGEAGEPGRVISAGANANTVAVSGPPGPPGAPGPAGPPGLSGPIGPAGLPGSPGAKGDKGDKGEDGKPGVTESAGTEKSEAGEPGPPGPPGPPGPPGDDGKPGVGEPGPAGPPGEKGEPGSFVPTSETFFAGPPGPPGPIGPKGSQGDVGPQGFPGQPGLPGEPGIGIPGQPGSPGPRGEQGLDGPLGPQGPQGPMGPMGPEGPKGDAGVPGAPGIPGYSYGGGSRSAPGPPGPPGPPGPPGEPGSQGDGSPNAVQQISDYLRSEPIRQYLAGPPGPPGPPGPSGSASDDALASRVIEYLQRDEVRQYLIGPPGPPGPPGVPGGYGFNTQEVAGRVMSLINDAGFRGLPGPPGPPGPQGPPGNSGGLVSYAANENQQHIRAEPQEYIKSHPRADENLDYTAIAKQVSDYIKAHGLLNGNAGFPGAPGRQGYPGPPGFPGEPGTPGQPGPPGQAGPPGLPGPPGLDTVHNYSNMTAVLDYIKENHMKGQKGDLGFPGIPGSPGIDGKQGIVGLPGIKGQKGERGEATYVNRRRRRSTVI

Target ORF information:

RefSeq Version	XM_005160112.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XVII, alpha 1a (col17a1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160112.4

ORF Insert Sequence:

ATGGACAAGC TAACAACAAC CAAGATTGTT AAAGTTGAGG GGGGATCTGG AGGATCTGGA 
AAAGAAATCC AGAAACATAC AGAAACTGTG ACGACTACAA GACTGACATC TCTACCGCCA 
AAAGCCCACA GCGGCCAGCA GATACTGACC TCCATCGGGA ACGGCTCAGG ATCTGCAGGA 
GGAGCTGTTT TGCTGGAGAA GAAGTTCATC ACTCACAGCA GCGCTGAAGG AGGAGCTTCC 
AGGAGTTATG TCATATCATC CTCCTCATCT GGAAGTTCAG CCAGCAGCAG CTCAGGAGCA 
GGATCTGTGA TCAGTGGATT TGACGGTATA TCTGGCGAAA TCAGTGTAAC CGGACCGATC 
ACTAGCTCTA CGGTTAAAAG CTCTACATCT GCAGTTAAAA GCTCTAGCGT GGTCTCTACC 
TCCATCACAT CCTCGTCAGC CGCAGGCGGA GAGCTTTCCT CTGGATACGG CTCCATCTCC 
TCATCCTCCT CCGAGTTCGT GTCTGGTTCC TCAGGAGGAT CCATCAGTAC TTCTGAAGGC 
AGCATGAGTA AATCTAGAAG CGGTGGAGGA TCATCTGCAG TCATGTCTGG CGAAGGAACA 
TCCAGCTCCA AGACTGTGTC CAAATTCAGC TCCTCGTCCC CTAGTGGAAC AAGGAAAGGT 
TTGAGTTACA GCACAGTTCC TACTGAGAGG AAGACCATTA TGAACATTAC CACAGGAGGA 
TATGAGGGGA GCTCCAGTGG CAATTCCTCC CCTGAGTACA TAAAGAAAGA GTACGTTTCC 
ACTAGCAGTG CTGCCACCAG AGGGCGCACT CAAACACGAG AGAGTGAGAT CAGAGCCAGA 
CTGCAGAGCG CCTCGCCCTC CGCTAGATGG ACAGAGTTGG ATGATGTGAA GAGATTACTG 
AAGGGAAGTC GCTCTGGGAG CATCAGTCCA CCACGTTCTC CCACCAGCAC TCTCCCTATC 
CCCAAAAAAG CCAGCATTGA CGTACGCCAC GAGAGCCAGT CAGGCTTTGA TGGCACAGTT 
TTGGATGCTG GATTCAGTGG ATATTACACC AATCCCAACA ATCTCAGTTA CACCACATTG 
CAACAACCAT CATCACCCAC AGGCGGCATG CAAAACAATC TGACCCTCAG TTCATCTGCC 
CTTGATACTA CAGTGTATGG GATGCAGAAT AACCTGTCTA CATCTGGCGG TGCTATGATG 
GCTAATGGAA GGTGCACTGG CTATGTGTAT GGGGTCCCGA AAAATGTTGG CAGCGCTGGA 
TTGGCCACTA CTATTTCTGC TATTCCAAGC AGTCCTATCA CCGCTGATGA CGTGTTTATG 
AAAGATGGCA AGTTAATTGC GATTGGGAAG GAAAATGTAG CGGCTAAGAC AGAGACCGAG 
AGGCTGATCA TGTCCAAAAA CACTGGCAAA CAGTTCGTCC CTTCATCCTT CAATGCTGGA 
GCAGACACGT TTTCTGAAGA TTCACTCAAG AGGGAGAAAA AGATGATCTC GAGCGCCACA 
GATCCACAGA CCACCACCAT TACTGGTTCC AAATCCTCAA CAAAAAACAA AGCAACATAT 
GCAGATATTC GTAAAGATGA TTCCGACGGC GCTGGATGTG GATTCTGGAC GTGTTGCTCG 
TGGTGGAAAT GGCTTCTGGC TTTGTTATTT GCTCTTCTCC TTCTGCTGGG ACTCCTCTGT 
GGACTGATCG CACTAGGTGA GCAGCTGAGG AGGCTGAAGG CTCGTGTGGA TGCTATCGAT 
GGTGGAGGAA CCTCTCCTCG AACCAGCGGT TTGAAAACTG ACGACATTCC TGGGGTCCAA 
TCTGGTAACA CGATAAACCT GGGTTATGAA CCTGCTGGGC AAGATTCGTT GATCCTGCAG 
AGAGCTATCC TGAGAGAGCT GCAGTCGGAC ACCATCAGAG CATCGCTGGC AACTTCGCTC 
AGGGGAGAGA GAGGAGAACC TGGACCTAAA GGAGACCAAG GGCCTCCTGG AGTTAAAGGT 
GATTCAGGAT TTCCCGGGAT TCCAGGTCCT CCTGGTCTTA TTGGTCATCC TGGACAGGAA 
GGCCCTCGAG GACCCAAAGG GAGCGCAGGT GAATCAGGAA GTGATGGTTT ACCTGGACCG 
AGGGGTCGTG AAGGTCCAGC GGGTCCCAGA GGAGAACCAG GACCCCCAGG AATTGGGGAG 
AAAGGAGAGA AAGGCTCATT TGGTGATGTT GGCGCTCCTG GGATAGCAGG ACCACCAGGA 
CCCGTAGGGC CCAAAGGTGA TGCAGGTGCA CAAGGAGTAC CGGGACTTAC AGGAGCACCT 
GGACCCCAAG GCTTCCGCGG AGATTCTGGT GATCCCGGAC CCAAGGGTGA TAAAGGACCT 
GCTGGGCCAC CTGGGGTCAA AGGTGATCAG GGAGAGAGAG GACCACGTGG CGCCACTGGT 
GAGCCGGGTC AGCCTGGACC AGCAGGTCCT CCTGGTGAGA AAGGTCTTAA GGGATCAACA 
GGTGCACCAG GACCAGATGG TGTTAAAGGA CTTAGGGGTG ACCAAGGACC CGTTGGGTTG 
CCTGGTGCTC GAGGACCATC AGGACCTCCC GGAGATTCAG GAATACCAGG AACACCAGGA 
CTCCAAGGAC CACCAGGACT GCCAGGTACA CCAGGCCAAC CAGGAGCTAA AGGTGAGGCC 
GGTGAGCCTG GAAGAGTCAT TTCTGCAGGC GCTAATGCTA ACACGGTGGC TGTATCTGGA 
CCACCAGGAC CTCCTGGAGC TCCAGGTCCT GCTGGTCCAC CTGGACTATC AGGTCCTATT 
GGTCCTGCTG GTCTTCCTGG TTCACCTGGT GCAAAGGGAG ATAAAGGAGA CAAAGGAGAG 
GATGGGAAAC CTGGAGTCAC TGAGAGTGCA GGGACAGAGA AATCAGAAGC AGGGGAACCC 
GGTCCTCCAG GACCACCAGG ACCTCCAGGA CCACCCGGGG ATGATGGAAA GCCAGGTGTT 
GGAGAACCTG GTCCAGCTGG TCCTCCTGGT GAAAAAGGTG AACCTGGAAG CTTTGTACCT 
ACTTCAGAAA CATTCTTTGC TGGACCACCT GGGCCTCCTG GACCTATTGG ACCAAAGGGA 
TCACAAGGTG ATGTTGGCCC TCAAGGTTTC CCTGGTCAAC CTGGATTACC TGGAGAACCT 
GGAATTGGTA TTCCAGGACA GCCGGGATCA CCAGGACCAC GTGGAGAGCA AGGTCTTGAT 
GGTCCACTTG GACCACAGGG ACCTCAAGGG CCAATGGGTC CCATGGGCCC AGAAGGTCCA 
AAAGGTGATG CTGGAGTCCC AGGTGCCCCT GGTATTCCAG GTTATTCTTA CGGTGGAGGA 
TCCAGAAGTG CTCCGGGACC ACCAGGTCCA CCTGGACCCC CAGGGCCTCC TGGAGAACCA 
GGTTCTCAAG GTGACGGGTC ACCGAATGCT GTTCAACAGA TTTCAGACTA CCTCAGAAGT 
GAGCCCATTA GACAATACCT AGCTGGACCT CCAGGCCCGC CTGGACCACC AGGTCCCTCT 
GGATCAGCCT CTGATGATGC TCTGGCTAGC AGAGTCATTG AATATCTTCA ACGAGATGAG 
GTGAGACAGT ACCTGATTGG TCCTCCAGGA CCCCCGGGAC CACCAGGTGT GCCAGGTGGA 
TATGGCTTTA ACACTCAGGA AGTGGCAGGA CGAGTGATGA GCCTTATTAA CGATGCTGGT 
TTTAGAGGTT TACCAGGACC TCCCGGCCCA CCAGGACCTC AAGGACCTCC TGGAAACTCT 
GGAGGACTGG TGTCTTACGC TGCTAACGAA AACCAGCAGC ACATCCGCGC AGAGCCGCAG 
GAGTACATCA AGAGCCATCC AAGGGCTGAT GAGAATTTGG ATTACACAGC CATCGCTAAA 
CAGGTTTCAG ATTACATTAA GGCTCATGGC CTGTTAAATG GAAATGCTGG CTTTCCAGGT 
GCACCAGGTC GTCAAGGATA TCCTGGACCA CCAGGGTTTC CAGGAGAGCC CGGAACACCA 
GGACAGCCTG GACCACCAGG ACAGGCTGGA CCACCAGGAC TGCCTGGACC ACCAGGACTC 
GACACTGTAC ACAATTACTC CAACATGACT GCTGTCTTGG ACTACATCAA AGAAAATCAT 
ATGAAAGGAC AAAAAGGCGA CCTGGGATTT CCAGGAATTC CAGGATCCCC AGGTATTGAT 
GGTAAACAAG GCATAGTAGG CTTACCAGGC ATAAAGGGAC AAAAAGGAGA AAGAGGTGAA 
GCAACATACG TCAACCGAAG GAGGAGGAGG AGCACAGTAA TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col17a1a ( XM_017354917.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210406.1 Danio rerio collagen, type XVII, alpha 1a (col17a1a), transcript variant X3, mRNA.

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CloneID	ODa06152
Clone ID Related Accession (Same CDS sequence)	XM_017354917.1 , XM_017354917.2
Accession Version	XM_017354917.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	collagen, type XVII, alpha 1a isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAAGC TAACAACAAC CAAGATTGTT AAAGTTGAGG GGGGATCTGG AGGATCTGGA 
AAAGAAATCC AGAAACATAC AGAAACTGTG ACGACTACAA GACTGACATC TCTACCGCCA 
AAAGCCCACA GCGGCCAGCA GATACTGACC TCCATCGGGA ACGGCTCAGG ATCTGCAGGA 
GGAGCTGTTT TGCTGGAGAA GAAGTTCATC ACTCACAGCA GCGCTGAAGG AGGAGCTTCC 
AGGAGTTATG TCATATCATC CTCCTCATCT GGAAGTTCAG CCAGCAGCAG CTCAGGAGCA 
GGATCTGTGA TCAGTGGATT TGACGGTATA TCTGGCGAAA TCAGTGTAAC CGGACCGATC 
ACTAGCTCTA CGGTTAAAAG CTCTACATCT GCAGTTAAAA GCTCTAGCGT GGTCTCTACC 
TCCATCACAT CCTCGTCAGC CGCAGGCGGA GAGCTTTCCT CTGGATACGG CTCCATCTCC 
TCATCCTCCT CCGAGTTCGT GTCTGGTTCC TCAGGAGGAT CCATCAGTAC TTCTGAAGGC 
AGCATGAGTA AATCTAGAAG CGGTGGAGGA TCATCTGCAG TCATGTCTGG CGAAGGAACA 
TCCAGCTCCA AGACTGTGTC CAAATTCAGC TCCTCGTCCC CTAGTGGAAC AAGGAAAGGT 
TTGAGTTACA GCACAGTTCC TACTGAGAGG AAGACCATTA TGAACATTAC CACAGGAGGA 
TATGAGGGGA GCTCCAGTGG CAATTCCTCC CCTGAGTACA TAAAGAAAGA GTACGTTTCC 
ACTAGCAGTG CTGCCACCAG AGGGCGCACT CAAACACGAG AGAGTGAGAT CAGAGCCAGA 
CTGCAGAGCG CCTCGCCCTC CGCTAGATGG ACAGAGTTGG ATGATGTGAA GAGATTACTG 
AAGGGAAGTC GCTCTGGGAG CATCAGTCCA CCACGTTCTC CCACCAGCAC TCTCCCTATC 
CCCAAAAAAG CCAGCATTGA CGTACGCCAC GAGAGCCAGT CAGGCTTTGA TGGCACAGTT 
TTGGATGCTG GATTCAGTGG ATATTACACC AATCCCAACA ATCTCAGTTA CACCACATTG 
CAACAACCAT CATCACCCAC AGGCGGCATG CAAAACAATC TGACCCTCAG TTCATCTGCC 
CTTGATACTA CAGTGTATGG GATGCAGAAT AACCTGTCTA CATCTGGCGG TGCTATGATG 
GCTAATGGAA GGTGCACTGG CTATGTGTAT GGGGTCCCGA AAAATGTTGG CAGCGCTGGA 
TTGGCCACTA CTATTTCTGC TATTCCAAGC AGTCCTATCA CCGCTGATGA CGTGTTTATG 
AAAGATGGCA AGTTAATTGC GATTGGGAAG GAAAATGTAG CGGCTAAGAC AGAGACCGAG 
AGGCTGATCA TGTCCAAAAA CACTGGCAAA CAGTTCGTCC CTTCATCCTT CAATGCTGGA 
GCAGACACGT TTTCTGAAGA TTCACTCAAG AGGGAGAAAA AGATGATCTC GAGCGCCACA 
GATCCACAGA CCACCACCAT TACTGGTTCC AAATCCTCAA CAAAAAACAA AGCAACATAT 
GCAGATATTC GTAAAGATGA TTCCGACGGC GCTGGATGTG GATTCTGGAC GTGTTGCTCG 
TGGTGGAAAT GGCTTCTGGC TTTGTTATTT GCTCTTCTCC TTCTGCTGGG ACTCCTCTGT 
GGACTGATCG CACTAGGTGA GCAGCTGAGG AGGCTGAAGG CTCGTGTGGA TGCTATCGAT 
GGTGGAGGAA CCTCTCCTCG AACCAGCGGT TTGAAAACTG ACGACATTCC TGGGGTCCAA 
TCTGGTAACA CGATAAACCT GGGTTATGAA CCTGCTGGGC AAGATTCGTT GATCCTGCAG 
AGAGCTATCC TGAGAGAGCT GCAGTCGGAC ACCATCAGAG CATCGCTGGC AACTTCGCTC 
AGGGGAGAGA GAGGAGAACC TGGACCTAAA GGAGACCAAG GGCCTCCTGG AGTTAAAGGT 
GATTCAGGAT TTCCCGGGAT TCCAGGTCCT CCTGGTCTTA TTGGTCATCC TGGACAGGAA 
GGCCCTCGAG GACCCAAAGG GAGCGCAGGT GAATCAGGAA GTGATGGTTT ACCTGGACCG 
AGGGGTCGTG AAGGTCCAGC GGGTCCCAGA GGAGAACCAG GACCCCCAGG AATTGGGGAG 
AAAGGAGAGA AAGGCTCATT TGGTGATGTT GGCGCTCCTG GGATAGCAGG ACCACCAGGA 
CCCGTAGGGC CCAAAGGTGA TGCAGGTGCA CAAGGAGTAC CGGGACTTAC AGGAGCACCT 
GGACCCCAAG GCTTCCGCGG AGATTCTGGT GATCCCGGAC CCAAGGGTGA TAAAGGACCT 
GCTGGGCCAC CTGGGGTCAA AGGTGATCAG GGAGAGAGAG GACCACGTGG CGCCACTGGT 
GAGCCGGGTC AGCCTGGACC AGCAGGTCCT CCTGGTGAGA AAGGTCTTAA GGGATCAACA 
GGTGCACCAG GACCAGATGG TGTTAAAGGA CTTAGGGGTG ACCAAGGACC CGTTGGGTTG 
CCTGGTGCTC GAGGACCATC AGGACCTCCC GGAGATTCAG GAATACCAGG AACACCAGGA 
CTCCAAGGAC CACCAGGACT GCCAGGTACA CCAGGCCAAC CAGGAGCTAA AGGTGAGGCC 
GGTGAGCCTG GAAGAGTCAT TTCTGCAGGC GCTAATGCTA ACACGGTGGC TGTATCTGGA 
CCACCAGGAC CTCCTGGAGC TCCAGGTCCT GCTGGTCCAC CTGGACTATC AGGTCCTATT 
GGTCCTGCTG GTCTTCCTGG TTCACCTGGT GCAAAGGGAG ATAAAGGAGA CAAAGGAGAG 
GATGGGAAAC CTGGAGTCAC TGAGAGTGCA GGGAAGAAAT CAGAAGCAGG GGAACCCGGT 
CCTCCAGGAC CACCAGGACC TCCAGGACCA CCCGGGGATG ATGGAAAGCC AGGTGTTGGA 
GAACCTGGTC CAGCTGGTCC TCCTGGTGAA AAAGGTGAAC CTGGAAGCTT TGTACCTACT 
TCAGAAACAT TCTTTGCTGG ACCACCTGGG CCTCCTGGAC CTATTGGACC AAAGGGATCA 
CAAGGTGATG TTGGCCCTCA AGGTTTCCCT GGTCAACCTG GATTACCTGG AGAACCTGGA 
ATTGGTATTC CAGGACAGCC GGGATCACCA GGACCACGTG GAGAGCAAGG TCTTGATGGT 
CCACTTGGAC CACAGGGACC TCAAGGGCCA ATGGGTCCCA TGGGCCCAGA AGGTCCAAAA 
GGTGATGCTG GAGTCCCAGG TGCCCCTGGT ATTCCAGGTT ATTCTTACGG TGGAGGATCC 
AGAAGTGCTC CGGGACCACC AGGTCCACCT GGACCCCCAG GGCCTCCTGG AGAACCAGGT 
TCTCAAGGTG ACGGGTCACC GAATGCTGTT CAACAGATTT CAGACTACCT CAGAAGTGAG 
CCCATTAGAC AATACCTAGC TGGACCTCCA GGCCCGCCTG GACCACCAGG TCCCTCTGGA 
TCAGCCTCTG ATGATGCTCT GGCTAGCAGA GTCATTGAAT ATCTTCAACG AGATGAGGTG 
AGACAGTACC TGATTGGTCC TCCAGGACCC CCGGGACCAC CAGGTGTGCC AGGTGGATAT 
GGCTTTAACA CTCAGGAAGT GGCAGGACGA GTGATGAGCC TTATTAACGA TGCTGGTTTT 
AGAGGTTTAC CAGGACCTCC CGGCCCACCA GGACCTCAAG GACCTCCTGG AAACTCTGGA 
GGACTGGTGT CTTACGCTGC TAACGAAAAC CAGCAGCACA TCCGCGCAGA GCCGCAGGAG 
TACATCAAGA GCCATCCAAG GGCTGATGAG AATTTGGATT ACACAGCCAT CGCTAAACAG 
GTTTCAGATT ACATTAAGGC TCATGGCCTG TTAAATGGAA ATGCTGGCTT TCCAGGTGCA 
CCAGGTCGTC AAGGATATCC TGGACCACCA GGGTTTCCAG GAGAGCCCGG AACACCAGGA 
CAGCCTGGAC CACCAGGACA GGCTGGACCA CCAGGACTGC CTGGACCACC AGGACTCGAC 
ACTGTACACA ATTACTCCAA CATGACTGCT GTCTTGGACT ACATCAAAGA AAATCATATG 
AAAGGACAAA AAGGCGACCT GGGATTTCCA GGAATTCCAG GATCCCCAGG TATTGATGGT 
AAACAAGGCA TAGTAGGCTT ACCAGGCATA AAGGGACAAA AAGGAGAAAG AGGTGAAGCA 
ACATACGTCA ACCGAAGGAG GAGGAGGAGC ACAGTAATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210406.1
CDS	181..4422
Translation	MDKLTTTKIVKVEGGSGGSGKEIQKHTETVTTTRLTSLPPKAHSGQQILTSIGNGSGSAGGAVLLEKKFITHSSAEGGASRSYVISSSSSGSSASSSSGAGSVISGFDGISGEISVTGPITSSTVKSSTSAVKSSSVVSTSITSSSAAGGELSSGYGSISSSSSEFVSGSSGGSISTSEGSMSKSRSGGGSSAVMSGEGTSSSKTVSKFSSSSPSGTRKGLSYSTVPTERKTIMNITTGGYEGSSSGNSSPEYIKKEYVSTSSAATRGRTQTRESEIRARLQSASPSARWTELDDVKRLLKGSRSGSISPPRSPTSTLPIPKKASIDVRHESQSGFDGTVLDAGFSGYYTNPNNLSYTTLQQPSSPTGGMQNNLTLSSSALDTTVYGMQNNLSTSGGAMMANGRCTGYVYGVPKNVGSAGLATTISAIPSSPITADDVFMKDGKLIAIGKENVAAKTETERLIMSKNTGKQFVPSSFNAGADTFSEDSLKREKKMISSATDPQTTTITGSKSSTKNKATYADIRKDDSDGAGCGFWTCCSWWKWLLALLFALLLLLGLLCGLIALGEQLRRLKARVDAIDGGGTSPRTSGLKTDDIPGVQSGNTINLGYEPAGQDSLILQRAILRELQSDTIRASLATSLRGERGEPGPKGDQGPPGVKGDSGFPGIPGPPGLIGHPGQEGPRGPKGSAGESGSDGLPGPRGREGPAGPRGEPGPPGIGEKGEKGSFGDVGAPGIAGPPGPVGPKGDAGAQGVPGLTGAPGPQGFRGDSGDPGPKGDKGPAGPPGVKGDQGERGPRGATGEPGQPGPAGPPGEKGLKGSTGAPGPDGVKGLRGDQGPVGLPGARGPSGPPGDSGIPGTPGLQGPPGLPGTPGQPGAKGEAGEPGRVISAGANANTVAVSGPPGPPGAPGPAGPPGLSGPIGPAGLPGSPGAKGDKGDKGEDGKPGVTESAGKKSEAGEPGPPGPPGPPGPPGDDGKPGVGEPGPAGPPGEKGEPGSFVPTSETFFAGPPGPPGPIGPKGSQGDVGPQGFPGQPGLPGEPGIGIPGQPGSPGPRGEQGLDGPLGPQGPQGPMGPMGPEGPKGDAGVPGAPGIPGYSYGGGSRSAPGPPGPPGPPGPPGEPGSQGDGSPNAVQQISDYLRSEPIRQYLAGPPGPPGPPGPSGSASDDALASRVIEYLQRDEVRQYLIGPPGPPGPPGVPGGYGFNTQEVAGRVMSLINDAGFRGLPGPPGPPGPQGPPGNSGGLVSYAANENQQHIRAEPQEYIKSHPRADENLDYTAIAKQVSDYIKAHGLLNGNAGFPGAPGRQGYPGPPGFPGEPGTPGQPGPPGQAGPPGLPGPPGLDTVHNYSNMTAVLDYIKENHMKGQKGDLGFPGIPGSPGIDGKQGIVGLPGIKGQKGERGEATYVNRRRRRSTVI

Target ORF information:

RefSeq Version	XM_017354917.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type XVII, alpha 1a (col17a1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354917.1

ORF Insert Sequence:

ATGGACAAGC TAACAACAAC CAAGATTGTT AAAGTTGAGG GGGGATCTGG AGGATCTGGA 
AAAGAAATCC AGAAACATAC AGAAACTGTG ACGACTACAA GACTGACATC TCTACCGCCA 
AAAGCCCACA GCGGCCAGCA GATACTGACC TCCATCGGGA ACGGCTCAGG ATCTGCAGGA 
GGAGCTGTTT TGCTGGAGAA GAAGTTCATC ACTCACAGCA GCGCTGAAGG AGGAGCTTCC 
AGGAGTTATG TCATATCATC CTCCTCATCT GGAAGTTCAG CCAGCAGCAG CTCAGGAGCA 
GGATCTGTGA TCAGTGGATT TGACGGTATA TCTGGCGAAA TCAGTGTAAC CGGACCGATC 
ACTAGCTCTA CGGTTAAAAG CTCTACATCT GCAGTTAAAA GCTCTAGCGT GGTCTCTACC 
TCCATCACAT CCTCGTCAGC CGCAGGCGGA GAGCTTTCCT CTGGATACGG CTCCATCTCC 
TCATCCTCCT CCGAGTTCGT GTCTGGTTCC TCAGGAGGAT CCATCAGTAC TTCTGAAGGC 
AGCATGAGTA AATCTAGAAG CGGTGGAGGA TCATCTGCAG TCATGTCTGG CGAAGGAACA 
TCCAGCTCCA AGACTGTGTC CAAATTCAGC TCCTCGTCCC CTAGTGGAAC AAGGAAAGGT 
TTGAGTTACA GCACAGTTCC TACTGAGAGG AAGACCATTA TGAACATTAC CACAGGAGGA 
TATGAGGGGA GCTCCAGTGG CAATTCCTCC CCTGAGTACA TAAAGAAAGA GTACGTTTCC 
ACTAGCAGTG CTGCCACCAG AGGGCGCACT CAAACACGAG AGAGTGAGAT CAGAGCCAGA 
CTGCAGAGCG CCTCGCCCTC CGCTAGATGG ACAGAGTTGG ATGATGTGAA GAGATTACTG 
AAGGGAAGTC GCTCTGGGAG CATCAGTCCA CCACGTTCTC CCACCAGCAC TCTCCCTATC 
CCCAAAAAAG CCAGCATTGA CGTACGCCAC GAGAGCCAGT CAGGCTTTGA TGGCACAGTT 
TTGGATGCTG GATTCAGTGG ATATTACACC AATCCCAACA ATCTCAGTTA CACCACATTG 
CAACAACCAT CATCACCCAC AGGCGGCATG CAAAACAATC TGACCCTCAG TTCATCTGCC 
CTTGATACTA CAGTGTATGG GATGCAGAAT AACCTGTCTA CATCTGGCGG TGCTATGATG 
GCTAATGGAA GGTGCACTGG CTATGTGTAT GGGGTCCCGA AAAATGTTGG CAGCGCTGGA 
TTGGCCACTA CTATTTCTGC TATTCCAAGC AGTCCTATCA CCGCTGATGA CGTGTTTATG 
AAAGATGGCA AGTTAATTGC GATTGGGAAG GAAAATGTAG CGGCTAAGAC AGAGACCGAG 
AGGCTGATCA TGTCCAAAAA CACTGGCAAA CAGTTCGTCC CTTCATCCTT CAATGCTGGA 
GCAGACACGT TTTCTGAAGA TTCACTCAAG AGGGAGAAAA AGATGATCTC GAGCGCCACA 
GATCCACAGA CCACCACCAT TACTGGTTCC AAATCCTCAA CAAAAAACAA AGCAACATAT 
GCAGATATTC GTAAAGATGA TTCCGACGGC GCTGGATGTG GATTCTGGAC GTGTTGCTCG 
TGGTGGAAAT GGCTTCTGGC TTTGTTATTT GCTCTTCTCC TTCTGCTGGG ACTCCTCTGT 
GGACTGATCG CACTAGGTGA GCAGCTGAGG AGGCTGAAGG CTCGTGTGGA TGCTATCGAT 
GGTGGAGGAA CCTCTCCTCG AACCAGCGGT TTGAAAACTG ACGACATTCC TGGGGTCCAA 
TCTGGTAACA CGATAAACCT GGGTTATGAA CCTGCTGGGC AAGATTCGTT GATCCTGCAG 
AGAGCTATCC TGAGAGAGCT GCAGTCGGAC ACCATCAGAG CATCGCTGGC AACTTCGCTC 
AGGGGAGAGA GAGGAGAACC TGGACCTAAA GGAGACCAAG GGCCTCCTGG AGTTAAAGGT 
GATTCAGGAT TTCCCGGGAT TCCAGGTCCT CCTGGTCTTA TTGGTCATCC TGGACAGGAA 
GGCCCTCGAG GACCCAAAGG GAGCGCAGGT GAATCAGGAA GTGATGGTTT ACCTGGACCG 
AGGGGTCGTG AAGGTCCAGC GGGTCCCAGA GGAGAACCAG GACCCCCAGG AATTGGGGAG 
AAAGGAGAGA AAGGCTCATT TGGTGATGTT GGCGCTCCTG GGATAGCAGG ACCACCAGGA 
CCCGTAGGGC CCAAAGGTGA TGCAGGTGCA CAAGGAGTAC CGGGACTTAC AGGAGCACCT 
GGACCCCAAG GCTTCCGCGG AGATTCTGGT GATCCCGGAC CCAAGGGTGA TAAAGGACCT 
GCTGGGCCAC CTGGGGTCAA AGGTGATCAG GGAGAGAGAG GACCACGTGG CGCCACTGGT 
GAGCCGGGTC AGCCTGGACC AGCAGGTCCT CCTGGTGAGA AAGGTCTTAA GGGATCAACA 
GGTGCACCAG GACCAGATGG TGTTAAAGGA CTTAGGGGTG ACCAAGGACC CGTTGGGTTG 
CCTGGTGCTC GAGGACCATC AGGACCTCCC GGAGATTCAG GAATACCAGG AACACCAGGA 
CTCCAAGGAC CACCAGGACT GCCAGGTACA CCAGGCCAAC CAGGAGCTAA AGGTGAGGCC 
GGTGAGCCTG GAAGAGTCAT TTCTGCAGGC GCTAATGCTA ACACGGTGGC TGTATCTGGA 
CCACCAGGAC CTCCTGGAGC TCCAGGTCCT GCTGGTCCAC CTGGACTATC AGGTCCTATT 
GGTCCTGCTG GTCTTCCTGG TTCACCTGGT GCAAAGGGAG ATAAAGGAGA CAAAGGAGAG 
GATGGGAAAC CTGGAGTCAC TGAGAGTGCA GGGAAGAAAT CAGAAGCAGG GGAACCCGGT 
CCTCCAGGAC CACCAGGACC TCCAGGACCA CCCGGGGATG ATGGAAAGCC AGGTGTTGGA 
GAACCTGGTC CAGCTGGTCC TCCTGGTGAA AAAGGTGAAC CTGGAAGCTT TGTACCTACT 
TCAGAAACAT TCTTTGCTGG ACCACCTGGG CCTCCTGGAC CTATTGGACC AAAGGGATCA 
CAAGGTGATG TTGGCCCTCA AGGTTTCCCT GGTCAACCTG GATTACCTGG AGAACCTGGA 
ATTGGTATTC CAGGACAGCC GGGATCACCA GGACCACGTG GAGAGCAAGG TCTTGATGGT 
CCACTTGGAC CACAGGGACC TCAAGGGCCA ATGGGTCCCA TGGGCCCAGA AGGTCCAAAA 
GGTGATGCTG GAGTCCCAGG TGCCCCTGGT ATTCCAGGTT ATTCTTACGG TGGAGGATCC 
AGAAGTGCTC CGGGACCACC AGGTCCACCT GGACCCCCAG GGCCTCCTGG AGAACCAGGT 
TCTCAAGGTG ACGGGTCACC GAATGCTGTT CAACAGATTT CAGACTACCT CAGAAGTGAG 
CCCATTAGAC AATACCTAGC TGGACCTCCA GGCCCGCCTG GACCACCAGG TCCCTCTGGA 
TCAGCCTCTG ATGATGCTCT GGCTAGCAGA GTCATTGAAT ATCTTCAACG AGATGAGGTG 
AGACAGTACC TGATTGGTCC TCCAGGACCC CCGGGACCAC CAGGTGTGCC AGGTGGATAT 
GGCTTTAACA CTCAGGAAGT GGCAGGACGA GTGATGAGCC TTATTAACGA TGCTGGTTTT 
AGAGGTTTAC CAGGACCTCC CGGCCCACCA GGACCTCAAG GACCTCCTGG AAACTCTGGA 
GGACTGGTGT CTTACGCTGC TAACGAAAAC CAGCAGCACA TCCGCGCAGA GCCGCAGGAG 
TACATCAAGA GCCATCCAAG GGCTGATGAG AATTTGGATT ACACAGCCAT CGCTAAACAG 
GTTTCAGATT ACATTAAGGC TCATGGCCTG TTAAATGGAA ATGCTGGCTT TCCAGGTGCA 
CCAGGTCGTC AAGGATATCC TGGACCACCA GGGTTTCCAG GAGAGCCCGG AACACCAGGA 
CAGCCTGGAC CACCAGGACA GGCTGGACCA CCAGGACTGC CTGGACCACC AGGACTCGAC 
ACTGTACACA ATTACTCCAA CATGACTGCT GTCTTGGACT ACATCAAAGA AAATCATATG 
AAAGGACAAA AAGGCGACCT GGGATTTCCA GGAATTCCAG GATCCCCAGG TATTGATGGT 
AAACAAGGCA TAGTAGGCTT ACCAGGCATA AAGGGACAAA AAGGAGAAAG AGGTGAAGCA 
ACATACGTCA ACCGAAGGAG GAGGAGGAGC ACAGTAATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col17a1a ( XM_017354917.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210406.1 Danio rerio collagen, type XVII, alpha 1a (col17a1a), transcript variant X3, mRNA.

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CloneID	ODa06152
Clone ID Related Accession (Same CDS sequence)	XM_017354917.1 , XM_017354917.2
Accession Version	XM_017354917.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen, type XVII, alpha 1a isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017354917.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAAGC TAACAACAAC CAAGATTGTT AAAGTTGAGG GGGGATCTGG AGGATCTGGA 
AAAGAAATCC AGAAACATAC AGAAACTGTG ACGACTACAA GACTGACATC TCTACCGCCA 
AAAGCCCACA GCGGCCAGCA GATACTGACC TCCATCGGGA ACGGCTCAGG ATCTGCAGGA 
GGAGCTGTTT TGCTGGAGAA GAAGTTCATC ACTCACAGCA GCGCTGAAGG AGGAGCTTCC 
AGGAGTTATG TCATATCATC CTCCTCATCT GGAAGTTCAG CCAGCAGCAG CTCAGGAGCA 
GGATCTGTGA TCAGTGGATT TGACGGTATA TCTGGCGAAA TCAGTGTAAC CGGACCGATC 
ACTAGCTCTA CGGTTAAAAG CTCTACATCT GCAGTTAAAA GCTCTAGCGT GGTCTCTACC 
TCCATCACAT CCTCGTCAGC CGCAGGCGGA GAGCTTTCCT CTGGATACGG CTCCATCTCC 
TCATCCTCCT CCGAGTTCGT GTCTGGTTCC TCAGGAGGAT CCATCAGTAC TTCTGAAGGC 
AGCATGAGTA AATCTAGAAG CGGTGGAGGA TCATCTGCAG TCATGTCTGG CGAAGGAACA 
TCCAGCTCCA AGACTGTGTC CAAATTCAGC TCCTCGTCCC CTAGTGGAAC AAGGAAAGGT 
TTGAGTTACA GCACAGTTCC TACTGAGAGG AAGACCATTA TGAACATTAC CACAGGAGGA 
TATGAGGGGA GCTCCAGTGG CAATTCCTCC CCTGAGTACA TAAAGAAAGA GTACGTTTCC 
ACTAGCAGTG CTGCCACCAG AGGGCGCACT CAAACACGAG AGAGTGAGAT CAGAGCCAGA 
CTGCAGAGCG CCTCGCCCTC CGCTAGATGG ACAGAGTTGG ATGATGTGAA GAGATTACTG 
AAGGGAAGTC GCTCTGGGAG CATCAGTCCA CCACGTTCTC CCACCAGCAC TCTCCCTATC 
CCCAAAAAAG CCAGCATTGA CGTACGCCAC GAGAGCCAGT CAGGCTTTGA TGGCACAGTT 
TTGGATGCTG GATTCAGTGG ATATTACACC AATCCCAACA ATCTCAGTTA CACCACATTG 
CAACAACCAT CATCACCCAC AGGCGGCATG CAAAACAATC TGACCCTCAG TTCATCTGCC 
CTTGATACTA CAGTGTATGG GATGCAGAAT AACCTGTCTA CATCTGGCGG TGCTATGATG 
GCTAATGGAA GGTGCACTGG CTATGTGTAT GGGGTCCCGA AAAATGTTGG CAGCGCTGGA 
TTGGCCACTA CTATTTCTGC TATTCCAAGC AGTCCTATCA CCGCTGATGA CGTGTTTATG 
AAAGATGGCA AGTTAATTGC GATTGGGAAG GAAAATGTAG CGGCTAAGAC AGAGACCGAG 
AGGCTGATCA TGTCCAAAAA CACTGGCAAA CAGTTCGTCC CTTCATCCTT CAATGCTGGA 
GCAGACACGT TTTCTGAAGA TTCACTCAAG AGGGAGAAAA AGATGATCTC GAGCGCCACA 
GATCCACAGA CCACCACCAT TACTGGTTCC AAATCCTCAA CAAAAAACAA AGCAACATAT 
GCAGATATTC GTAAAGATGA TTCCGACGGC GCTGGATGTG GATTCTGGAC GTGTTGCTCG 
TGGTGGAAAT GGCTTCTGGC TTTGTTATTT GCTCTTCTCC TTCTGCTGGG ACTCCTCTGT 
GGACTGATCG CACTAGGTGA GCAGCTGAGG AGGCTGAAGG CTCGTGTGGA TGCTATCGAT 
GGTGGAGGAA CCTCTCCTCG AACCAGCGGT TTGAAAACTG ACGACATTCC TGGGGTCCAA 
TCTGGTAACA CGATAAACCT GGGTTATGAA CCTGCTGGGC AAGATTCGTT GATCCTGCAG 
AGAGCTATCC TGAGAGAGCT GCAGTCGGAC ACCATCAGAG CATCGCTGGC AACTTCGCTC 
AGGGGAGAGA GAGGAGAACC TGGACCTAAA GGAGACCAAG GGCCTCCTGG AGTTAAAGGT 
GATTCAGGAT TTCCCGGGAT TCCAGGTCCT CCTGGTCTTA TTGGTCATCC TGGACAGGAA 
GGCCCTCGAG GACCCAAAGG GAGCGCAGGT GAATCAGGAA GTGATGGTTT ACCTGGACCG 
AGGGGTCGTG AAGGTCCAGC GGGTCCCAGA GGAGAACCAG GACCCCCAGG AATTGGGGAG 
AAAGGAGAGA AAGGCTCATT TGGTGATGTT GGCGCTCCTG GGATAGCAGG ACCACCAGGA 
CCCGTAGGGC CCAAAGGTGA TGCAGGTGCA CAAGGAGTAC CGGGACTTAC AGGAGCACCT 
GGACCCCAAG GCTTCCGCGG AGATTCTGGT GATCCCGGAC CCAAGGGTGA TAAAGGACCT 
GCTGGGCCAC CTGGGGTCAA AGGTGATCAG GGAGAGAGAG GACCACGTGG CGCCACTGGT 
GAGCCGGGTC AGCCTGGACC AGCAGGTCCT CCTGGTGAGA AAGGTCTTAA GGGATCAACA 
GGTGCACCAG GACCAGATGG TGTTAAAGGA CTTAGGGGTG ACCAAGGACC CGTTGGGTTG 
CCTGGTGCTC GAGGACCATC AGGACCTCCC GGAGATTCAG GAATACCAGG AACACCAGGA 
CTCCAAGGAC CACCAGGACT GCCAGGTACA CCAGGCCAAC CAGGAGCTAA AGGTGAGGCC 
GGTGAGCCTG GAAGAGTCAT TTCTGCAGGC GCTAATGCTA ACACGGTGGC TGTATCTGGA 
CCACCAGGAC CTCCTGGAGC TCCAGGTCCT GCTGGTCCAC CTGGACTATC AGGTCCTATT 
GGTCCTGCTG GTCTTCCTGG TTCACCTGGT GCAAAGGGAG ATAAAGGAGA CAAAGGAGAG 
GATGGGAAAC CTGGAGTCAC TGAGAGTGCA GGGAAGAAAT CAGAAGCAGG GGAACCCGGT 
CCTCCAGGAC CACCAGGACC TCCAGGACCA CCCGGGGATG ATGGAAAGCC AGGTGTTGGA 
GAACCTGGTC CAGCTGGTCC TCCTGGTGAA AAAGGTGAAC CTGGAAGCTT TGTACCTACT 
TCAGAAACAT TCTTTGCTGG ACCACCTGGG CCTCCTGGAC CTATTGGACC AAAGGGATCA 
CAAGGTGATG TTGGCCCTCA AGGTTTCCCT GGTCAACCTG GATTACCTGG AGAACCTGGA 
ATTGGTATTC CAGGACAGCC GGGATCACCA GGACCACGTG GAGAGCAAGG TCTTGATGGT 
CCACTTGGAC CACAGGGACC TCAAGGGCCA ATGGGTCCCA TGGGCCCAGA AGGTCCAAAA 
GGTGATGCTG GAGTCCCAGG TGCCCCTGGT ATTCCAGGTT ATTCTTACGG TGGAGGATCC 
AGAAGTGCTC CGGGACCACC AGGTCCACCT GGACCCCCAG GGCCTCCTGG AGAACCAGGT 
TCTCAAGGTG ACGGGTCACC GAATGCTGTT CAACAGATTT CAGACTACCT CAGAAGTGAG 
CCCATTAGAC AATACCTAGC TGGACCTCCA GGCCCGCCTG GACCACCAGG TCCCTCTGGA 
TCAGCCTCTG ATGATGCTCT GGCTAGCAGA GTCATTGAAT ATCTTCAACG AGATGAGGTG 
AGACAGTACC TGATTGGTCC TCCAGGACCC CCGGGACCAC CAGGTGTGCC AGGTGGATAT 
GGCTTTAACA CTCAGGAAGT GGCAGGACGA GTGATGAGCC TTATTAACGA TGCTGGTTTT 
AGAGGTTTAC CAGGACCTCC CGGCCCACCA GGACCTCAAG GACCTCCTGG AAACTCTGGA 
GGACTGGTGT CTTACGCTGC TAACGAAAAC CAGCAGCACA TCCGCGCAGA GCCGCAGGAG 
TACATCAAGA GCCATCCAAG GGCTGATGAG AATTTGGATT ACACAGCCAT CGCTAAACAG 
GTTTCAGATT ACATTAAGGC TCATGGCCTG TTAAATGGAA ATGCTGGCTT TCCAGGTGCA 
CCAGGTCGTC AAGGATATCC TGGACCACCA GGGTTTCCAG GAGAGCCCGG AACACCAGGA 
CAGCCTGGAC CACCAGGACA GGCTGGACCA CCAGGACTGC CTGGACCACC AGGACTCGAC 
ACTGTACACA ATTACTCCAA CATGACTGCT GTCTTGGACT ACATCAAAGA AAATCATATG 
AAAGGACAAA AAGGCGACCT GGGATTTCCA GGAATTCCAG GATCCCCAGG TATTGATGGT 
AAACAAGGCA TAGTAGGCTT ACCAGGCATA AAGGGACAAA AAGGAGAAAG AGGTGAAGCA 
ACATACGTCA ACCGAAGGAG GAGGAGGAGC ACAGTAATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210406.1
CDS	185..4426
Translation	MDKLTTTKIVKVEGGSGGSGKEIQKHTETVTTTRLTSLPPKAHSGQQILTSIGNGSGSAGGAVLLEKKFITHSSAEGGASRSYVISSSSSGSSASSSSGAGSVISGFDGISGEISVTGPITSSTVKSSTSAVKSSSVVSTSITSSSAAGGELSSGYGSISSSSSEFVSGSSGGSISTSEGSMSKSRSGGGSSAVMSGEGTSSSKTVSKFSSSSPSGTRKGLSYSTVPTERKTIMNITTGGYEGSSSGNSSPEYIKKEYVSTSSAATRGRTQTRESEIRARLQSASPSARWTELDDVKRLLKGSRSGSISPPRSPTSTLPIPKKASIDVRHESQSGFDGTVLDAGFSGYYTNPNNLSYTTLQQPSSPTGGMQNNLTLSSSALDTTVYGMQNNLSTSGGAMMANGRCTGYVYGVPKNVGSAGLATTISAIPSSPITADDVFMKDGKLIAIGKENVAAKTETERLIMSKNTGKQFVPSSFNAGADTFSEDSLKREKKMISSATDPQTTTITGSKSSTKNKATYADIRKDDSDGAGCGFWTCCSWWKWLLALLFALLLLLGLLCGLIALGEQLRRLKARVDAIDGGGTSPRTSGLKTDDIPGVQSGNTINLGYEPAGQDSLILQRAILRELQSDTIRASLATSLRGERGEPGPKGDQGPPGVKGDSGFPGIPGPPGLIGHPGQEGPRGPKGSAGESGSDGLPGPRGREGPAGPRGEPGPPGIGEKGEKGSFGDVGAPGIAGPPGPVGPKGDAGAQGVPGLTGAPGPQGFRGDSGDPGPKGDKGPAGPPGVKGDQGERGPRGATGEPGQPGPAGPPGEKGLKGSTGAPGPDGVKGLRGDQGPVGLPGARGPSGPPGDSGIPGTPGLQGPPGLPGTPGQPGAKGEAGEPGRVISAGANANTVAVSGPPGPPGAPGPAGPPGLSGPIGPAGLPGSPGAKGDKGDKGEDGKPGVTESAGKKSEAGEPGPPGPPGPPGPPGDDGKPGVGEPGPAGPPGEKGEPGSFVPTSETFFAGPPGPPGPIGPKGSQGDVGPQGFPGQPGLPGEPGIGIPGQPGSPGPRGEQGLDGPLGPQGPQGPMGPMGPEGPKGDAGVPGAPGIPGYSYGGGSRSAPGPPGPPGPPGPPGEPGSQGDGSPNAVQQISDYLRSEPIRQYLAGPPGPPGPPGPSGSASDDALASRVIEYLQRDEVRQYLIGPPGPPGPPGVPGGYGFNTQEVAGRVMSLINDAGFRGLPGPPGPPGPQGPPGNSGGLVSYAANENQQHIRAEPQEYIKSHPRADENLDYTAIAKQVSDYIKAHGLLNGNAGFPGAPGRQGYPGPPGFPGEPGTPGQPGPPGQAGPPGLPGPPGLDTVHNYSNMTAVLDYIKENHMKGQKGDLGFPGIPGSPGIDGKQGIVGLPGIKGQKGERGEATYVNRRRRRSTVI

Target ORF information:

RefSeq Version	XM_017354917.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XVII, alpha 1a (col17a1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354917.2

ORF Insert Sequence:

ATGGACAAGC TAACAACAAC CAAGATTGTT AAAGTTGAGG GGGGATCTGG AGGATCTGGA 
AAAGAAATCC AGAAACATAC AGAAACTGTG ACGACTACAA GACTGACATC TCTACCGCCA 
AAAGCCCACA GCGGCCAGCA GATACTGACC TCCATCGGGA ACGGCTCAGG ATCTGCAGGA 
GGAGCTGTTT TGCTGGAGAA GAAGTTCATC ACTCACAGCA GCGCTGAAGG AGGAGCTTCC 
AGGAGTTATG TCATATCATC CTCCTCATCT GGAAGTTCAG CCAGCAGCAG CTCAGGAGCA 
GGATCTGTGA TCAGTGGATT TGACGGTATA TCTGGCGAAA TCAGTGTAAC CGGACCGATC 
ACTAGCTCTA CGGTTAAAAG CTCTACATCT GCAGTTAAAA GCTCTAGCGT GGTCTCTACC 
TCCATCACAT CCTCGTCAGC CGCAGGCGGA GAGCTTTCCT CTGGATACGG CTCCATCTCC 
TCATCCTCCT CCGAGTTCGT GTCTGGTTCC TCAGGAGGAT CCATCAGTAC TTCTGAAGGC 
AGCATGAGTA AATCTAGAAG CGGTGGAGGA TCATCTGCAG TCATGTCTGG CGAAGGAACA 
TCCAGCTCCA AGACTGTGTC CAAATTCAGC TCCTCGTCCC CTAGTGGAAC AAGGAAAGGT 
TTGAGTTACA GCACAGTTCC TACTGAGAGG AAGACCATTA TGAACATTAC CACAGGAGGA 
TATGAGGGGA GCTCCAGTGG CAATTCCTCC CCTGAGTACA TAAAGAAAGA GTACGTTTCC 
ACTAGCAGTG CTGCCACCAG AGGGCGCACT CAAACACGAG AGAGTGAGAT CAGAGCCAGA 
CTGCAGAGCG CCTCGCCCTC CGCTAGATGG ACAGAGTTGG ATGATGTGAA GAGATTACTG 
AAGGGAAGTC GCTCTGGGAG CATCAGTCCA CCACGTTCTC CCACCAGCAC TCTCCCTATC 
CCCAAAAAAG CCAGCATTGA CGTACGCCAC GAGAGCCAGT CAGGCTTTGA TGGCACAGTT 
TTGGATGCTG GATTCAGTGG ATATTACACC AATCCCAACA ATCTCAGTTA CACCACATTG 
CAACAACCAT CATCACCCAC AGGCGGCATG CAAAACAATC TGACCCTCAG TTCATCTGCC 
CTTGATACTA CAGTGTATGG GATGCAGAAT AACCTGTCTA CATCTGGCGG TGCTATGATG 
GCTAATGGAA GGTGCACTGG CTATGTGTAT GGGGTCCCGA AAAATGTTGG CAGCGCTGGA 
TTGGCCACTA CTATTTCTGC TATTCCAAGC AGTCCTATCA CCGCTGATGA CGTGTTTATG 
AAAGATGGCA AGTTAATTGC GATTGGGAAG GAAAATGTAG CGGCTAAGAC AGAGACCGAG 
AGGCTGATCA TGTCCAAAAA CACTGGCAAA CAGTTCGTCC CTTCATCCTT CAATGCTGGA 
GCAGACACGT TTTCTGAAGA TTCACTCAAG AGGGAGAAAA AGATGATCTC GAGCGCCACA 
GATCCACAGA CCACCACCAT TACTGGTTCC AAATCCTCAA CAAAAAACAA AGCAACATAT 
GCAGATATTC GTAAAGATGA TTCCGACGGC GCTGGATGTG GATTCTGGAC GTGTTGCTCG 
TGGTGGAAAT GGCTTCTGGC TTTGTTATTT GCTCTTCTCC TTCTGCTGGG ACTCCTCTGT 
GGACTGATCG CACTAGGTGA GCAGCTGAGG AGGCTGAAGG CTCGTGTGGA TGCTATCGAT 
GGTGGAGGAA CCTCTCCTCG AACCAGCGGT TTGAAAACTG ACGACATTCC TGGGGTCCAA 
TCTGGTAACA CGATAAACCT GGGTTATGAA CCTGCTGGGC AAGATTCGTT GATCCTGCAG 
AGAGCTATCC TGAGAGAGCT GCAGTCGGAC ACCATCAGAG CATCGCTGGC AACTTCGCTC 
AGGGGAGAGA GAGGAGAACC TGGACCTAAA GGAGACCAAG GGCCTCCTGG AGTTAAAGGT 
GATTCAGGAT TTCCCGGGAT TCCAGGTCCT CCTGGTCTTA TTGGTCATCC TGGACAGGAA 
GGCCCTCGAG GACCCAAAGG GAGCGCAGGT GAATCAGGAA GTGATGGTTT ACCTGGACCG 
AGGGGTCGTG AAGGTCCAGC GGGTCCCAGA GGAGAACCAG GACCCCCAGG AATTGGGGAG 
AAAGGAGAGA AAGGCTCATT TGGTGATGTT GGCGCTCCTG GGATAGCAGG ACCACCAGGA 
CCCGTAGGGC CCAAAGGTGA TGCAGGTGCA CAAGGAGTAC CGGGACTTAC AGGAGCACCT 
GGACCCCAAG GCTTCCGCGG AGATTCTGGT GATCCCGGAC CCAAGGGTGA TAAAGGACCT 
GCTGGGCCAC CTGGGGTCAA AGGTGATCAG GGAGAGAGAG GACCACGTGG CGCCACTGGT 
GAGCCGGGTC AGCCTGGACC AGCAGGTCCT CCTGGTGAGA AAGGTCTTAA GGGATCAACA 
GGTGCACCAG GACCAGATGG TGTTAAAGGA CTTAGGGGTG ACCAAGGACC CGTTGGGTTG 
CCTGGTGCTC GAGGACCATC AGGACCTCCC GGAGATTCAG GAATACCAGG AACACCAGGA 
CTCCAAGGAC CACCAGGACT GCCAGGTACA CCAGGCCAAC CAGGAGCTAA AGGTGAGGCC 
GGTGAGCCTG GAAGAGTCAT TTCTGCAGGC GCTAATGCTA ACACGGTGGC TGTATCTGGA 
CCACCAGGAC CTCCTGGAGC TCCAGGTCCT GCTGGTCCAC CTGGACTATC AGGTCCTATT 
GGTCCTGCTG GTCTTCCTGG TTCACCTGGT GCAAAGGGAG ATAAAGGAGA CAAAGGAGAG 
GATGGGAAAC CTGGAGTCAC TGAGAGTGCA GGGAAGAAAT CAGAAGCAGG GGAACCCGGT 
CCTCCAGGAC CACCAGGACC TCCAGGACCA CCCGGGGATG ATGGAAAGCC AGGTGTTGGA 
GAACCTGGTC CAGCTGGTCC TCCTGGTGAA AAAGGTGAAC CTGGAAGCTT TGTACCTACT 
TCAGAAACAT TCTTTGCTGG ACCACCTGGG CCTCCTGGAC CTATTGGACC AAAGGGATCA 
CAAGGTGATG TTGGCCCTCA AGGTTTCCCT GGTCAACCTG GATTACCTGG AGAACCTGGA 
ATTGGTATTC CAGGACAGCC GGGATCACCA GGACCACGTG GAGAGCAAGG TCTTGATGGT 
CCACTTGGAC CACAGGGACC TCAAGGGCCA ATGGGTCCCA TGGGCCCAGA AGGTCCAAAA 
GGTGATGCTG GAGTCCCAGG TGCCCCTGGT ATTCCAGGTT ATTCTTACGG TGGAGGATCC 
AGAAGTGCTC CGGGACCACC AGGTCCACCT GGACCCCCAG GGCCTCCTGG AGAACCAGGT 
TCTCAAGGTG ACGGGTCACC GAATGCTGTT CAACAGATTT CAGACTACCT CAGAAGTGAG 
CCCATTAGAC AATACCTAGC TGGACCTCCA GGCCCGCCTG GACCACCAGG TCCCTCTGGA 
TCAGCCTCTG ATGATGCTCT GGCTAGCAGA GTCATTGAAT ATCTTCAACG AGATGAGGTG 
AGACAGTACC TGATTGGTCC TCCAGGACCC CCGGGACCAC CAGGTGTGCC AGGTGGATAT 
GGCTTTAACA CTCAGGAAGT GGCAGGACGA GTGATGAGCC TTATTAACGA TGCTGGTTTT 
AGAGGTTTAC CAGGACCTCC CGGCCCACCA GGACCTCAAG GACCTCCTGG AAACTCTGGA 
GGACTGGTGT CTTACGCTGC TAACGAAAAC CAGCAGCACA TCCGCGCAGA GCCGCAGGAG 
TACATCAAGA GCCATCCAAG GGCTGATGAG AATTTGGATT ACACAGCCAT CGCTAAACAG 
GTTTCAGATT ACATTAAGGC TCATGGCCTG TTAAATGGAA ATGCTGGCTT TCCAGGTGCA 
CCAGGTCGTC AAGGATATCC TGGACCACCA GGGTTTCCAG GAGAGCCCGG AACACCAGGA 
CAGCCTGGAC CACCAGGACA GGCTGGACCA CCAGGACTGC CTGGACCACC AGGACTCGAC 
ACTGTACACA ATTACTCCAA CATGACTGCT GTCTTGGACT ACATCAAAGA AAATCATATG 
AAAGGACAAA AAGGCGACCT GGGATTTCCA GGAATTCCAG GATCCCCAGG TATTGATGGT 
AAACAAGGCA TAGTAGGCTT ACCAGGCATA AAGGGACAAA AAGGAGAAAG AGGTGAAGCA 
ACATACGTCA ACCGAAGGAG GAGGAGGAGC ACAGTAATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col17a1a ( XM_021474972.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330647.1 Danio rerio collagen, type XVII, alpha 1a (col17a1a), transcript variant X4, mRNA.

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CloneID	ODa147251
Clone ID Related Accession (Same CDS sequence)	XM_021474972.1
Accession Version	XM_021474972.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen, type XVII, alpha 1a isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAAGC TAACAACAAC CAAGATTGTT AAAGTTGAGG GGGGATCTGG AGGATCTGGA 
AAAGAAATCC AGAAACATAC AGAAACTGTG ACGACTACAA GACTGACATC TCTACCGCCA 
AAAGCCCACA GCGGCCAGCA GATACTGACC TCCATCGGGA ACGGCTCAGG ATCTGCAGGA 
GGAGCTGTTT TGCTGGAGAA GAAGTTCATC ACTCACAGCA GCGCTGAAGG AGGAGCTTCC 
AGGAGTTATG TCATATCATC CTCCTCATCT GGAAGTTCAG CCAGCAGCAG CTCAGGAGCA 
GGATCTGTGA TCAGTGGATT TGACGGTATA TCTGGCGAAA TCAGTGTAAC CGGACCGATC 
ACTAGCTCTA CGGTTAAAAG CTCTACATCT GCAGTTAAAA GCTCTAGCGT GGTCTCTACC 
TCCATCACAT CCTCGTCAGC CGCAGGCGGA GAGCTTTCCT CTGGATACGG CTCCATCTCC 
TCATCCTCCT CCGAGTTCGT GTCTGGTTCC TCAGGAGGAT CCATCAGTAC TTCTGAAGGC 
AGCATGAGTA AATCTAGAAG CGGTGGAGGA TCATCTGCAG TCATGTCTGG CGAAGGAACA 
TCCAGCTCCA AGACTGTGTC CAAATTCAGC TCCTCGTCCC CTAGTGGAAC AAGGAAAGGT 
TTGAGTTACA GCACAGTTCC TACTGAGAGG AAGACCATTA TGAACATTAC CACAGGAGGA 
TATGAGGGGA GCTCCAGTGG CAATTCCTCC CCTGAGTACA TAAAGAAAGA GTACGTTTCC 
ACTAGCAGTG CTGCCACCAG AGGGCGCACT CAAACACGAG AGAGTGAGAT CAGAGCCAGA 
CTGCAGAGCG CCTCGCCCTC CGCTAGATGG ACAGAGTTGG ATGATGTGAA GAGATTACTG 
AAGGGAAGTC GCTCTGGGAG CATCAGTCCA CCACGTTCTC CCACCAGCAC TCTCCCTATC 
CCCAAAAAAG CCAGCATTGA CGTACGCCAC GAGAGCCAGT CAGGCTTTGA TGGCACAGTT 
TTGGATGCTG GATTCAGTGG ATATTACACC AATCCCAACA ATCTCAGTTA CACCACATTG 
CAACAACCAT CATCACCCAC AGTGTATGGG ATGCAGAATA ACCTGTCTAC ATCTGGCGGT 
GCTATGATGG CTAATGGAAG GTGCACTGGC TATGTGTATG GGGTCCCGAA AAATGTTGGC 
AGCGCTGGAT TGGCCACTAC TATTTCTGCT ATTCCAAGCA GTCCTATCAC CGCTGATGAC 
GTGTTTATGA AAGATGGCAA GTTAATTGCG ATTGGGAAGG AAAATGTAGC GGCTAAGACA 
GAGACCGAGA GGCTGATCAT GTCCAAAAAC ACTGGCAAAC AGTTCGTCCC TTCATCCTTC 
AATGCTGGAG CAGACACGTT TTCTGAAGAT TCACTCAAGA GGGAGAAAAA GATGATCTCG 
AGCGCCACAG ATCCACAGAC CACCACCATT ACTGGTTCCA AATCCTCAAC AAAAAACAAA 
GCAACATATG CAGATATTCG TAAAGATGAT TCCGACGGCG CTGGATGTGG ATTCTGGACG 
TGTTGCTCGT GGTGGAAATG GCTTCTGGCT TTGTTATTTG CTCTTCTCCT TCTGCTGGGA 
CTCCTCTGTG GACTGATCGC ACTAGGTGAG CAGCTGAGGA GGCTGAAGGC TCGTGTGGAT 
GCTATCGATG GTGGAGGAAC CTCTCCTCGA ACCAGCGGTT TGAAAACTGA CGACATTCCT 
GGGGTCCAAT CTGGTAACAC GATAAACCTG GGTTATGAAC CTGCTGGGCA AGATTCGTTG 
ATCCTGCAGA GAGCTATCCT GAGAGAGCTG CAGTCGGACA CCATCAGAGC ATCGCTGGCA 
ACTTCGCTCA GGGGAGAGAG AGGAGAACCT GGACCTAAAG GAGACCAAGG GCCTCCTGGA 
GTTAAAGGTG ATTCAGGATT TCCCGGGATT CCAGGTCCTC CTGGTCTTAT TGGTCATCCT 
GGACAGGAAG GCCCTCGAGG ACCCAAAGGG AGCGCAGGTG AATCAGGAAG TGATGGTTTA 
CCTGGACCGA GGGGTCGTGA AGGTCCAGCG GGTCCCAGAG GAGAACCAGG ACCCCCAGGA 
ATTGGGGAGA AAGGAGAGAA AGGCTCATTT GGTGATGTTG GCGCTCCTGG GATAGCAGGA 
CCACCAGGAC CCGTAGGGCC CAAAGGTGAT GCAGGTGCAC AAGGAGTACC GGGACTTACA 
GGAGCACCTG GACCCCAAGG CTTCCGCGGA GATTCTGGTG ATCCCGGACC CAAGGGTGAT 
AAAGGACCTG CTGGGCCACC TGGGGTCAAA GGTGATCAGG GAGAGAGAGG ACCACGTGGC 
GCCACTGGTG AGCCGGGTCA GCCTGGACCA GCAGGTCCTC CTGGTGAGAA AGGTCTTAAG 
GGATCAACAG GTGCACCAGG ACCAGATGGT GTTAAAGGAC TTAGGGGTGA CCAAGGACCC 
GTTGGGTTGC CTGGTGCTCG AGGACCATCA GGACCTCCCG GAGATTCAGG AATACCAGGA 
ACACCAGGAC TCCAAGGACC ACCAGGACTG CCAGGTACAC CAGGCCAACC AGGAGCTAAA 
GGTGAGGCCG GTGAGCCTGG AAGAGTCATT TCTGCAGGCG CTAATGCTAA CACGGTGGCT 
GTATCTGGAC CACCAGGACC TCCTGGAGCT CCAGGTCCTG CTGGTCCACC TGGACTATCA 
GGTCCTATTG GTCCTGCTGG TCTTCCTGGT TCACCTGGTG CAAAGGGAGA TAAAGGAGAC 
AAAGGAGAGG ATGGGAAACC TGGAGTCACT GAGAGTGCAG GGACAGAGAA ATCAGAAGCA 
GGGGAACCCG GTCCTCCAGG ACCACCAGGA CCTCCAGGAC CACCCGGGGA TGATGGAAAG 
CCAGGTGTTG GAGAACCTGG TCCAGCTGGT CCTCCTGGTG AAAAAGGTGA ACCTGGAAGC 
TTTGTACCTA CTTCAGAAAC ATTCTTTGCT GGACCACCTG GGCCTCCTGG ACCTATTGGA 
CCAAAGGGAT CACAAGGTGA TGTTGGCCCT CAAGGTTTCC CTGGTCAACC TGGATTACCT 
GGAGAACCTG GAATTGGTAT TCCAGGACAG CCGGGATCAC CAGGACCACG TGGAGAGCAA 
GGTCTTGATG GTCCACTTGG ACCACAGGGA CCTCAAGGGC CAATGGGTCC CATGGGCCCA 
GAAGGTCCAA AAGGTGATGC TGGAGTCCCA GGTGCCCCTG GTATTCCAGG TTATTCTTAC 
GGTGGAGGAT CCAGAAGTGC TCCGGGACCA CCAGGTCCAC CTGGACCCCC AGGGCCTCCT 
GGAGAACCAG GTTCTCAAGG TGACGGGTCA CCGAATGCTG TTCAACAGAT TTCAGACTAC 
CTCAGAAGTG AGCCCATTAG ACAATACCTA GCTGGACCTC CAGGCCCGCC TGGACCACCA 
GGTCCCTCTG GATCAGCCTC TGATGATGCT CTGGCTAGCA GAGTCATTGA ATATCTTCAA 
CGAGATGAGG TGAGACAGTA CCTGATTGGT CCTCCAGGAC CCCCGGGACC ACCAGGTGTG 
CCAGGTGGAT ATGGCTTTAA CACTCAGGAA GTGGCAGGAC GAGTGATGAG CCTTATTAAC 
GATGCTGGTT TTAGAGGTTT ACCAGGACCT CCCGGCCCAC CAGGACCTCA AGGACCTCCT 
GGAAACTCTG GAGGACTGGT GTCTTACGCT GCTAACGAAA ACCAGCAGCA CATCCGCGCA 
GAGCCGCAGG AGTACATCAA GAGCCATCCA AGGGCTGATG AGAATTTGGA TTACACAGCC 
ATCGCTAAAC AGGTTTCAGA TTACATTAAG GCTCATGGCC TGTTAAATGG AAATGCTGGC 
TTTCCAGGTG CACCAGGTCG TCAAGGATAT CCTGGACCAC CAGGGTTTCC AGGAGAGCCC 
GGAACACCAG GACAGCCTGG ACCACCAGGA CAGGCTGGAC CACCAGGACT GCCTGGACCA 
CCAGGACTCG ACACTGTACA CAATTACTCC AACATGACTG CTGTCTTGGA CTACATCAAA 
GAAAATCATA TGAAAGGACA AAAAGGCGAC CTGGGATTTC CAGGAATTCC AGGATCCCCA 
GGTATTGATG GTAAACAAGG CATAGTAGGC TTACCAGGCA TAAAGGGACA AAAAGGAGAA 
AGAGGTGAAG CAACATACGT CAACCGAAGG AGGAGGAGGA GCACAGTAAT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330647.1
CDS	186..4379
Translation	MDKLTTTKIVKVEGGSGGSGKEIQKHTETVTTTRLTSLPPKAHSGQQILTSIGNGSGSAGGAVLLEKKFITHSSAEGGASRSYVISSSSSGSSASSSSGAGSVISGFDGISGEISVTGPITSSTVKSSTSAVKSSSVVSTSITSSSAAGGELSSGYGSISSSSSEFVSGSSGGSISTSEGSMSKSRSGGGSSAVMSGEGTSSSKTVSKFSSSSPSGTRKGLSYSTVPTERKTIMNITTGGYEGSSSGNSSPEYIKKEYVSTSSAATRGRTQTRESEIRARLQSASPSARWTELDDVKRLLKGSRSGSISPPRSPTSTLPIPKKASIDVRHESQSGFDGTVLDAGFSGYYTNPNNLSYTTLQQPSSPTVYGMQNNLSTSGGAMMANGRCTGYVYGVPKNVGSAGLATTISAIPSSPITADDVFMKDGKLIAIGKENVAAKTETERLIMSKNTGKQFVPSSFNAGADTFSEDSLKREKKMISSATDPQTTTITGSKSSTKNKATYADIRKDDSDGAGCGFWTCCSWWKWLLALLFALLLLLGLLCGLIALGEQLRRLKARVDAIDGGGTSPRTSGLKTDDIPGVQSGNTINLGYEPAGQDSLILQRAILRELQSDTIRASLATSLRGERGEPGPKGDQGPPGVKGDSGFPGIPGPPGLIGHPGQEGPRGPKGSAGESGSDGLPGPRGREGPAGPRGEPGPPGIGEKGEKGSFGDVGAPGIAGPPGPVGPKGDAGAQGVPGLTGAPGPQGFRGDSGDPGPKGDKGPAGPPGVKGDQGERGPRGATGEPGQPGPAGPPGEKGLKGSTGAPGPDGVKGLRGDQGPVGLPGARGPSGPPGDSGIPGTPGLQGPPGLPGTPGQPGAKGEAGEPGRVISAGANANTVAVSGPPGPPGAPGPAGPPGLSGPIGPAGLPGSPGAKGDKGDKGEDGKPGVTESAGTEKSEAGEPGPPGPPGPPGPPGDDGKPGVGEPGPAGPPGEKGEPGSFVPTSETFFAGPPGPPGPIGPKGSQGDVGPQGFPGQPGLPGEPGIGIPGQPGSPGPRGEQGLDGPLGPQGPQGPMGPMGPEGPKGDAGVPGAPGIPGYSYGGGSRSAPGPPGPPGPPGPPGEPGSQGDGSPNAVQQISDYLRSEPIRQYLAGPPGPPGPPGPSGSASDDALASRVIEYLQRDEVRQYLIGPPGPPGPPGVPGGYGFNTQEVAGRVMSLINDAGFRGLPGPPGPPGPQGPPGNSGGLVSYAANENQQHIRAEPQEYIKSHPRADENLDYTAIAKQVSDYIKAHGLLNGNAGFPGAPGRQGYPGPPGFPGEPGTPGQPGPPGQAGPPGLPGPPGLDTVHNYSNMTAVLDYIKENHMKGQKGDLGFPGIPGSPGIDGKQGIVGLPGIKGQKGERGEATYVNRRRRRSTVI

Target ORF information:

RefSeq Version	XM_021474972.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XVII, alpha 1a (col17a1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474972.1

ORF Insert Sequence:

ATGGACAAGC TAACAACAAC CAAGATTGTT AAAGTTGAGG GGGGATCTGG AGGATCTGGA 
AAAGAAATCC AGAAACATAC AGAAACTGTG ACGACTACAA GACTGACATC TCTACCGCCA 
AAAGCCCACA GCGGCCAGCA GATACTGACC TCCATCGGGA ACGGCTCAGG ATCTGCAGGA 
GGAGCTGTTT TGCTGGAGAA GAAGTTCATC ACTCACAGCA GCGCTGAAGG AGGAGCTTCC 
AGGAGTTATG TCATATCATC CTCCTCATCT GGAAGTTCAG CCAGCAGCAG CTCAGGAGCA 
GGATCTGTGA TCAGTGGATT TGACGGTATA TCTGGCGAAA TCAGTGTAAC CGGACCGATC 
ACTAGCTCTA CGGTTAAAAG CTCTACATCT GCAGTTAAAA GCTCTAGCGT GGTCTCTACC 
TCCATCACAT CCTCGTCAGC CGCAGGCGGA GAGCTTTCCT CTGGATACGG CTCCATCTCC 
TCATCCTCCT CCGAGTTCGT GTCTGGTTCC TCAGGAGGAT CCATCAGTAC TTCTGAAGGC 
AGCATGAGTA AATCTAGAAG CGGTGGAGGA TCATCTGCAG TCATGTCTGG CGAAGGAACA 
TCCAGCTCCA AGACTGTGTC CAAATTCAGC TCCTCGTCCC CTAGTGGAAC AAGGAAAGGT 
TTGAGTTACA GCACAGTTCC TACTGAGAGG AAGACCATTA TGAACATTAC CACAGGAGGA 
TATGAGGGGA GCTCCAGTGG CAATTCCTCC CCTGAGTACA TAAAGAAAGA GTACGTTTCC 
ACTAGCAGTG CTGCCACCAG AGGGCGCACT CAAACACGAG AGAGTGAGAT CAGAGCCAGA 
CTGCAGAGCG CCTCGCCCTC CGCTAGATGG ACAGAGTTGG ATGATGTGAA GAGATTACTG 
AAGGGAAGTC GCTCTGGGAG CATCAGTCCA CCACGTTCTC CCACCAGCAC TCTCCCTATC 
CCCAAAAAAG CCAGCATTGA CGTACGCCAC GAGAGCCAGT CAGGCTTTGA TGGCACAGTT 
TTGGATGCTG GATTCAGTGG ATATTACACC AATCCCAACA ATCTCAGTTA CACCACATTG 
CAACAACCAT CATCACCCAC AGTGTATGGG ATGCAGAATA ACCTGTCTAC ATCTGGCGGT 
GCTATGATGG CTAATGGAAG GTGCACTGGC TATGTGTATG GGGTCCCGAA AAATGTTGGC 
AGCGCTGGAT TGGCCACTAC TATTTCTGCT ATTCCAAGCA GTCCTATCAC CGCTGATGAC 
GTGTTTATGA AAGATGGCAA GTTAATTGCG ATTGGGAAGG AAAATGTAGC GGCTAAGACA 
GAGACCGAGA GGCTGATCAT GTCCAAAAAC ACTGGCAAAC AGTTCGTCCC TTCATCCTTC 
AATGCTGGAG CAGACACGTT TTCTGAAGAT TCACTCAAGA GGGAGAAAAA GATGATCTCG 
AGCGCCACAG ATCCACAGAC CACCACCATT ACTGGTTCCA AATCCTCAAC AAAAAACAAA 
GCAACATATG CAGATATTCG TAAAGATGAT TCCGACGGCG CTGGATGTGG ATTCTGGACG 
TGTTGCTCGT GGTGGAAATG GCTTCTGGCT TTGTTATTTG CTCTTCTCCT TCTGCTGGGA 
CTCCTCTGTG GACTGATCGC ACTAGGTGAG CAGCTGAGGA GGCTGAAGGC TCGTGTGGAT 
GCTATCGATG GTGGAGGAAC CTCTCCTCGA ACCAGCGGTT TGAAAACTGA CGACATTCCT 
GGGGTCCAAT CTGGTAACAC GATAAACCTG GGTTATGAAC CTGCTGGGCA AGATTCGTTG 
ATCCTGCAGA GAGCTATCCT GAGAGAGCTG CAGTCGGACA CCATCAGAGC ATCGCTGGCA 
ACTTCGCTCA GGGGAGAGAG AGGAGAACCT GGACCTAAAG GAGACCAAGG GCCTCCTGGA 
GTTAAAGGTG ATTCAGGATT TCCCGGGATT CCAGGTCCTC CTGGTCTTAT TGGTCATCCT 
GGACAGGAAG GCCCTCGAGG ACCCAAAGGG AGCGCAGGTG AATCAGGAAG TGATGGTTTA 
CCTGGACCGA GGGGTCGTGA AGGTCCAGCG GGTCCCAGAG GAGAACCAGG ACCCCCAGGA 
ATTGGGGAGA AAGGAGAGAA AGGCTCATTT GGTGATGTTG GCGCTCCTGG GATAGCAGGA 
CCACCAGGAC CCGTAGGGCC CAAAGGTGAT GCAGGTGCAC AAGGAGTACC GGGACTTACA 
GGAGCACCTG GACCCCAAGG CTTCCGCGGA GATTCTGGTG ATCCCGGACC CAAGGGTGAT 
AAAGGACCTG CTGGGCCACC TGGGGTCAAA GGTGATCAGG GAGAGAGAGG ACCACGTGGC 
GCCACTGGTG AGCCGGGTCA GCCTGGACCA GCAGGTCCTC CTGGTGAGAA AGGTCTTAAG 
GGATCAACAG GTGCACCAGG ACCAGATGGT GTTAAAGGAC TTAGGGGTGA CCAAGGACCC 
GTTGGGTTGC CTGGTGCTCG AGGACCATCA GGACCTCCCG GAGATTCAGG AATACCAGGA 
ACACCAGGAC TCCAAGGACC ACCAGGACTG CCAGGTACAC CAGGCCAACC AGGAGCTAAA 
GGTGAGGCCG GTGAGCCTGG AAGAGTCATT TCTGCAGGCG CTAATGCTAA CACGGTGGCT 
GTATCTGGAC CACCAGGACC TCCTGGAGCT CCAGGTCCTG CTGGTCCACC TGGACTATCA 
GGTCCTATTG GTCCTGCTGG TCTTCCTGGT TCACCTGGTG CAAAGGGAGA TAAAGGAGAC 
AAAGGAGAGG ATGGGAAACC TGGAGTCACT GAGAGTGCAG GGACAGAGAA ATCAGAAGCA 
GGGGAACCCG GTCCTCCAGG ACCACCAGGA CCTCCAGGAC CACCCGGGGA TGATGGAAAG 
CCAGGTGTTG GAGAACCTGG TCCAGCTGGT CCTCCTGGTG AAAAAGGTGA ACCTGGAAGC 
TTTGTACCTA CTTCAGAAAC ATTCTTTGCT GGACCACCTG GGCCTCCTGG ACCTATTGGA 
CCAAAGGGAT CACAAGGTGA TGTTGGCCCT CAAGGTTTCC CTGGTCAACC TGGATTACCT 
GGAGAACCTG GAATTGGTAT TCCAGGACAG CCGGGATCAC CAGGACCACG TGGAGAGCAA 
GGTCTTGATG GTCCACTTGG ACCACAGGGA CCTCAAGGGC CAATGGGTCC CATGGGCCCA 
GAAGGTCCAA AAGGTGATGC TGGAGTCCCA GGTGCCCCTG GTATTCCAGG TTATTCTTAC 
GGTGGAGGAT CCAGAAGTGC TCCGGGACCA CCAGGTCCAC CTGGACCCCC AGGGCCTCCT 
GGAGAACCAG GTTCTCAAGG TGACGGGTCA CCGAATGCTG TTCAACAGAT TTCAGACTAC 
CTCAGAAGTG AGCCCATTAG ACAATACCTA GCTGGACCTC CAGGCCCGCC TGGACCACCA 
GGTCCCTCTG GATCAGCCTC TGATGATGCT CTGGCTAGCA GAGTCATTGA ATATCTTCAA 
CGAGATGAGG TGAGACAGTA CCTGATTGGT CCTCCAGGAC CCCCGGGACC ACCAGGTGTG 
CCAGGTGGAT ATGGCTTTAA CACTCAGGAA GTGGCAGGAC GAGTGATGAG CCTTATTAAC 
GATGCTGGTT TTAGAGGTTT ACCAGGACCT CCCGGCCCAC CAGGACCTCA AGGACCTCCT 
GGAAACTCTG GAGGACTGGT GTCTTACGCT GCTAACGAAA ACCAGCAGCA CATCCGCGCA 
GAGCCGCAGG AGTACATCAA GAGCCATCCA AGGGCTGATG AGAATTTGGA TTACACAGCC 
ATCGCTAAAC AGGTTTCAGA TTACATTAAG GCTCATGGCC TGTTAAATGG AAATGCTGGC 
TTTCCAGGTG CACCAGGTCG TCAAGGATAT CCTGGACCAC CAGGGTTTCC AGGAGAGCCC 
GGAACACCAG GACAGCCTGG ACCACCAGGA CAGGCTGGAC CACCAGGACT GCCTGGACCA 
CCAGGACTCG ACACTGTACA CAATTACTCC AACATGACTG CTGTCTTGGA CTACATCAAA 
GAAAATCATA TGAAAGGACA AAAAGGCGAC CTGGGATTTC CAGGAATTCC AGGATCCCCA 
GGTATTGATG GTAAACAAGG CATAGTAGGC TTACCAGGCA TAAAGGGACA AAAAGGAGAA 
AGAGGTGAAG CAACATACGT CAACCGAAGG AGGAGGAGGA GCACAGTAAT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

A COL17A1 Splice-Altering Mutation Is Prevalent in Inherited Recurrent Corneal Erosions.
Ophthalmology123(4)709-22(2016 Apr)
Oliver VF,van Bysterveldt KA,Cadzow M,Steger B,Romano V,Markie D,Hewitt AW,Mackey DA,Willoughby CE,Sherwin T,Crosier PS,McGhee CN,Vincent AL

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Zebrafish: a model system to study heritable skin diseases.
The Journal of investigative dermatology131(3)565-71(2011 Mar)
Li Q,Frank M,Thisse CI,Thisse BV,Uitto J

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col17a1a cDNA ORF clone, Danio rerio(zebrafish)

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ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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