ALS2A cDNA ORF clone, Danio rerio(Zebrafish)

The following ALS2A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALS2A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa14960 XM_679972.8
Latest version!
Danio rerio amyotrophic lateral sclerosis 2a (juvenile) (als2a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa14960 XM_021478854.1
Latest version!
Danio rerio amyotrophic lateral sclerosis 2a (juvenile) (als2a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
ODa14960 XM_679972.7 Danio rerio amyotrophic lateral sclerosis 2a (juvenile) (als2a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa14960
    Clone ID Related Accession (Same CDS sequence) XM_679972.8 , XM_021478854.1 , XM_679972.7
    Accession Version XM_679972.8 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4989bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product alsin
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394743.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_679972.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTGATGG AGGAACAGGG CAGGTCTGGA GAACAGGATG GCTCTAGAGA GAAAGGTGTG 
    CTCTACAGAT GGCAGGGCTA TTTCTGCAGT GTGAGCCCGG AGAAGCTGCT CCTGTCGAGG
    CCAGTACTCC ATGCTGCATT GGGGTCTTTA CATGGACTTC TGCTGATAGA AGGTGGCCAA
    GTATATAGTT TTGGTGAGCA GCCGTGGAAG CCAGTTGAAC CTCCGCCTGC CAGTCTTGTT
    TTAGAGAGCA CCCTGAGTGG TCAGCATGTG ATCTCTGTGT CTGCGGGCAG CTATCACTGC
    TCTGCTGTAA CAGAGGATGG TCTGGTCCTC ATGTGGGGCG AGAACTCATA CGGCCAGTGT
    GGAGTCTCTG GAACAGACCG GGTCCCCAGT CCCACACCCG TTACTGTGGT GGATGACGAG
    ACTCATCCTC CACAACTGGT CCGAGTCCTA AATGTGGCTT GCGGAGCACA GCACACTCTG
    GCTCTGTCAA ACAAGCATGA GGTGTGGGCC TGGGGCAGCG GTTGCCAGCT AGGACTGGTC
    ACCAATGTAT TCCCAGTCTG GAAACCCCAA AAGGTGGAGC ATCTAGTGGG AAGATATGTA
    GTTCAGATTG CCTGTGGAGC GTTTCACAGC CTGGCTTTGG TTCAGTTTCT GCCTTCCCCA
    GATGCAAGCC AGCAAGCTCA GGATAAATGT GGGCAGTGCA ATCAGATCCG TTACACCATG
    ACTGACAAGG AAGACCATGT TGTTATTTCC GATGAGCACT ACTGCCCACT TGGAGTGGAA
    CTGGCAGAAT CTAAAGAAGG TCGAAAGTCA GGACAGAGTT CTCCAGCTCA AACACCACAA
    AGCAAAAACT CATCGCCGAA TTACCAAGTG TCACCTCTAA CCTCAACGGA CACCTTTTCA
    TCTTCTTTTC AGCAAAATAC CACAAACCAG GAGACACAAC CAGACCCTTC TCTATCTGAG
    ACTGGACCTG CATGTCTTCA GCGATCCAAA AGCACTCCCT ACCCTGATGA GCAGGCTCTG
    AAAGACTACC TCAAAAAAAT TTTAGACCAG ACTCTTGCTG AGCAGGCAAC ATCAGCAGGA
    GGTTCCCATC CTCCGAGCCG TCAGTCATCA CTAACATGTC CTTTCGGATC AGAGCAGAGT
    GATGAACTTA CACTACGTCC CTCTTCACCA GCACTGAGCA GGACTGACAG CATCACATCC
    TCTGCCAGAA AACACATCAG TGCTTCAGAT ACTGTTACAG AGCAGAAAAA CCAACATATA
    ATACCAACGC TGGACTCCAC TTCTGATGAT TCTTCTCAGC ACTCCAGTGT GGATGGAGGC
    ATTTATCTCA GTGACCTCGT GATTAGCAAT GGGTGTTTGT TTGAATCTTG TGAGGAGGGT
    GGAGATACTG CTGGGACTGA CTTCTGTCCA GCTGATCTGC TTCAAAGCAA GAAAAGCATC
    AGTCTGACGG ATGTTACCTT GGAGGACACT GATCGCAATA GCTTCTCTCC AGTCTCGCCT
    TCTGACACTC ACCGAACCAG CAATTTGAGA GCAGTGAGAG CTTCTACCTC AGAGTCCAGA
    AATCTCCTTC CTTCCCTCTT CACGGAGGTG TGGAGTTGGG GGAAAGGTCA CGAAGGTCAG
    CTTGGTCATG GAGACTATTT GCCCAGGCTA CAGCCTCTCT GCATCAAGAG CTTAAGCAAA
    AAGGAAGTTG TCAAGATCAC AGCTGGAGCA AATCACTCCT TGGCGCTGAC AGCTCAATGT
    CAGGTTTATT CATGGGGAAG TGAAAAATTT GGCCAGCTGG GTCGTATGAA TTCTCCCAGC
    ACAGTTCCTA ATCTCACCAC GGTGTCAGAT GGTATTCGCG TCTGGGATGT GGCAGCAGGA
    CAAACGCACA CTTTGCTATT GGCCGATGGA GACTGTTATC AGCCCATACT GTACTACAGC
    GGAGAGCAGG TGCTGGAGGA CGCAGACAGC AGGACCTGCA CTCAGACACC CACCCTTCTG
    CCTTTCTGCA TGAATATTGG CTACGTGAAC CAGGTTTTTG GGGGAGGACT GAATTGTTTG
    GCTTTGGCCG ATCAGAATGC AATGGGTTTT ATATCAACAA TCCATGAGCT TGCATCGGCA
    GAGAGGAAAT TCTACTGCAG CCTCAGTAAA CTGAAGAACC AGATCCTGAG CCCTCTGTTA
    GCCCGTGCAA GTTTGGCCTT GTCTCTGGGT GAGTCGTCCA TGTTGCTCTT TCAAGAAATG
    GCTGGTAGTT TCAGCAAGCT TTGCCATCTG ACAGGGCAAC ACTCAGTGTC CCTCACCAAC
    TTCCTGCAGC AGGGCAAAGT GATCGAAACG CTGGTCTTGA TCAAACACAC AGCCATTTTT
    ATGGATACCT ATAAAGAGTA CAGTGGTCCG GTTGGAAACT TTCTTGTTAT GGGAGGCTTC
    CAGTCATTGC TGAAACCCTC ACAAGAATGC TTTGGGAAGA AACTGGAGTT GATACAGGCT
    CTACTAGAAT CCAAGGAGAA GAACCCGTCG CTCTATGATC TGTTAGTAAA CTTGTTTTAC
    ATGCCCTTGC AACACTTGAA TGGGTATAAC AGGTTACTTC TGAAACTAGC CAACTGTTTT
    GAAGTGAACA CTGATGAATA CCAGAGGATT CAAAATGGCT GCTCCAAATA TGAGGCTTTA
    GCTCGCCTTG TCAGCAAGCA GAGAAAAGAT GCTGAGGCCA CTTTCCATTT CTGGAAGAAC
    TTCAAAGGAA AGAATTTGGA AGCTCTGCGT AAGCCGTCCC GTCGTCTGGT ATGTGAGAGC
    AGTAATAAAG GCCTAACGTT ACAGAACGCT GGAAGATTTT CAGCCAGCTG GTTCATTCTC
    TTCAATGACG TGCTGGTTCA TGCACAGGGA AGCATTCCAT CAAAGAAGCT TTTCTCATCT
    CATTATGTCT ATCCACTTGC TACACTCTGG GTGGAGCCCA TCTCAGATGA AAGCTTGGGA
    TTACTTGGCC TTAAGCTGAC CACACCTGAA GATAGCTTTG TTGTGCTGGC AACGTCCCCT
    CTGGAAAAGG GCAAGTGGCT GAGAGCCATT AACCAGGCCA TTGATGAAGT GCTGGTGTCA
    GGCATGAACG GGAGGACGCG TGTTTCTAGA AGCAGCCAGA AAGGTGAACC TCCTGTGTCC
    CGTACTTCCA ACTACACCTT CAGCAAAGAG GGCCGACTTA AAGATGCCAA ATATGAAGGC
    CGCTGGCTGT CAGGCAAACC TCACGGCAAA GGGAATTTGA AGTGGCCTGA TGGGACAATG
    TACTGTGGCA CATTCAAGAG TGGACTGGAA GATGGGTTTG GAGATTTTAT GACGCCCAGT
    ACCACCTTCA ACAAGTTTGA GCGTTACCAA GGTCACTGGA AGGAAGGAAA AATGCATGGC
    TTTGGCACTT TCTGGTATGC TTCAGGTGAA GTCTATGAGG GCTCTTTCAG AGAGAACATG
    CGTCATGGTC ACGGCATGTT ACGCAGTGGG AAAGTGGCCT CTCCCTCGTC CAGTAGTGTG
    TTTGTGGGTC AGTGGGTTCA AGGGAAGAGG ACAGGCTATG GGGTGTGTGA TGACTTCAGC
    AGAGGAGAGA AGTACATGGG GATATGGCAT GATGACCAGC GTCACGGAGA CGGGGTTGTT
    ATCACACAAT TCGGATTGTA TTATGAAGGA AACTTCTGTA ATAACAGAAT GATGGGCAAT
    GGGACTCTTC TTTGTGATGA CGACACAGTC TTTGAGGGTG ACTTTCAGGA TGACTGGACA
    CTGTGTGGAA AGGGAATTCT GTTCATGCCT AATGGAGACT CTTTTGATGG TGTCTTTGAC
    GGCCACTGGG GGTCTGGACT GAGAGTGGCT GGAACTTTTA CAAAACCCTT TGAAATAGCA
    AGGAGAGATA TCAAGATGCC TGTGGCTGGT CAACATGCAG TTCCTGCAGA AGAAAAGTGG
    AAAGCCGTCT TTGAGGAATG CTGGAGCCGA CTGGGCTGTG AAACCGCTGG TCAGGGGGCA
    TTGGAGAAAG CCTGGGAGAA CATCGCCATC TCCCTCACTG CCAAACGCAG ACAACAGAGA
    GATCGTTCAG AAGAGTTGAG CCGATCACAA ACTAAGATGC TGGAGTGTCT TGAGGTGATT
    CCTCTGCATG AGGGCCCTGT TACTGCAAAT TCCTACAACA ACATCCGTCG GTATCTTGTA
    AAGGCTTGTG ATACCCCTCT GCACCCACTG GGCTGGCTGC TGGAGACCCT TGTCACCGTG
    TACAGGATGA CTTATGTTGG AGTGGGCTCT AGCAGACGCC TCCTGCAGCA GGCAGTGGAG
    GAGATCCAGT CATACCTAAC ACGCATTTTC CACATAGTCA GGTTTCTGTT TCCTGGTCTC
    CCTGAGGATG GAGGTTTTAT TGTGGACTCG TCTTGTTCCT CAGATGAGGG CACAGACTCT
    CTGAACAGCA GTACTCATTC AGAAGCTCCA CAGCAGGGTT GGACCGTGAG CAGCTCAAGT
    CTCCTCCTGC CGGTGCTACT GCCGAGGCTC TACCCTCCAC TCTTCACCCT CTATACTTTG
    GAGAAAGAGA GAGAGGAGGA GCTGTACTGG AACTGTGTGT TAAGACTCAA CAAACAGCCT
    GATCTAGCCC TTCTTGCATT CCTTGGGGTG CAACAAAAAT TCTGGCCGGT TTCTATCTCT
    GTATTGGGGG AAATAAAGAA GGTTCTGCCT AGTACTAAAG ACGCCTGCTT TTCCACTGCT
    GTTGAAACCC TGCAACAGAT CAGCACAGCA TTCACTCCTT CAGACAAGTT GCAGGTGATA
    CAGCTGACTT TTGAGGAGGT CACCCAGGAC GTGCAGGCTA TTCTTTGCCA GGACTTTTTG
    TGGTGCATGG ACGACCTCTT TCCAATTTTC CTCTATGTGG TGCTGCGTGC ACGGATCAGA
    AACCTGGGCT CTGAAGTGAG TCTGATAGAA GACCTCACAG ACAGCAATCT ACAGCTGGGA
    CAACTGGGAT TCATGCTGAC AACTCTCAAA GCCTGTTACA ACCAGATTCA GCTTGAGAAG
    ACTACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_685064.6
    CDS135..5123
    Translation

    Target ORF information:

    RefSeq Version XM_679972.8
    Organism Danio rerio(zebrafish)
    Definition Danio rerio amyotrophic lateral sclerosis 2a (juvenile) (als2a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_679972.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTGATGG AGGAACAGGG CAGGTCTGGA GAACAGGATG GCTCTAGAGA GAAAGGTGTG 
    CTCTACAGAT GGCAGGGCTA TTTCTGCAGT GTGAGCCCGG AGAAGCTGCT CCTGTCGAGG
    CCAGTACTCC ATGCTGCATT GGGGTCTTTA CATGGACTTC TGCTGATAGA AGGTGGCCAA
    GTATATAGTT TTGGTGAGCA GCCGTGGAAG CCAGTTGAAC CTCCGCCTGC CAGTCTTGTT
    TTAGAGAGCA CCCTGAGTGG TCAGCATGTG ATCTCTGTGT CTGCGGGCAG CTATCACTGC
    TCTGCTGTAA CAGAGGATGG TCTGGTCCTC ATGTGGGGCG AGAACTCATA CGGCCAGTGT
    GGAGTCTCTG GAACAGACCG GGTCCCCAGT CCCACACCCG TTACTGTGGT GGATGACGAG
    ACTCATCCTC CACAACTGGT CCGAGTCCTA AATGTGGCTT GCGGAGCACA GCACACTCTG
    GCTCTGTCAA ACAAGCATGA GGTGTGGGCC TGGGGCAGCG GTTGCCAGCT AGGACTGGTC
    ACCAATGTAT TCCCAGTCTG GAAACCCCAA AAGGTGGAGC ATCTAGTGGG AAGATATGTA
    GTTCAGATTG CCTGTGGAGC GTTTCACAGC CTGGCTTTGG TTCAGTTTCT GCCTTCCCCA
    GATGCAAGCC AGCAAGCTCA GGATAAATGT GGGCAGTGCA ATCAGATCCG TTACACCATG
    ACTGACAAGG AAGACCATGT TGTTATTTCC GATGAGCACT ACTGCCCACT TGGAGTGGAA
    CTGGCAGAAT CTAAAGAAGG TCGAAAGTCA GGACAGAGTT CTCCAGCTCA AACACCACAA
    AGCAAAAACT CATCGCCGAA TTACCAAGTG TCACCTCTAA CCTCAACGGA CACCTTTTCA
    TCTTCTTTTC AGCAAAATAC CACAAACCAG GAGACACAAC CAGACCCTTC TCTATCTGAG
    ACTGGACCTG CATGTCTTCA GCGATCCAAA AGCACTCCCT ACCCTGATGA GCAGGCTCTG
    AAAGACTACC TCAAAAAAAT TTTAGACCAG ACTCTTGCTG AGCAGGCAAC ATCAGCAGGA
    GGTTCCCATC CTCCGAGCCG TCAGTCATCA CTAACATGTC CTTTCGGATC AGAGCAGAGT
    GATGAACTTA CACTACGTCC CTCTTCACCA GCACTGAGCA GGACTGACAG CATCACATCC
    TCTGCCAGAA AACACATCAG TGCTTCAGAT ACTGTTACAG AGCAGAAAAA CCAACATATA
    ATACCAACGC TGGACTCCAC TTCTGATGAT TCTTCTCAGC ACTCCAGTGT GGATGGAGGC
    ATTTATCTCA GTGACCTCGT GATTAGCAAT GGGTGTTTGT TTGAATCTTG TGAGGAGGGT
    GGAGATACTG CTGGGACTGA CTTCTGTCCA GCTGATCTGC TTCAAAGCAA GAAAAGCATC
    AGTCTGACGG ATGTTACCTT GGAGGACACT GATCGCAATA GCTTCTCTCC AGTCTCGCCT
    TCTGACACTC ACCGAACCAG CAATTTGAGA GCAGTGAGAG CTTCTACCTC AGAGTCCAGA
    AATCTCCTTC CTTCCCTCTT CACGGAGGTG TGGAGTTGGG GGAAAGGTCA CGAAGGTCAG
    CTTGGTCATG GAGACTATTT GCCCAGGCTA CAGCCTCTCT GCATCAAGAG CTTAAGCAAA
    AAGGAAGTTG TCAAGATCAC AGCTGGAGCA AATCACTCCT TGGCGCTGAC AGCTCAATGT
    CAGGTTTATT CATGGGGAAG TGAAAAATTT GGCCAGCTGG GTCGTATGAA TTCTCCCAGC
    ACAGTTCCTA ATCTCACCAC GGTGTCAGAT GGTATTCGCG TCTGGGATGT GGCAGCAGGA
    CAAACGCACA CTTTGCTATT GGCCGATGGA GACTGTTATC AGCCCATACT GTACTACAGC
    GGAGAGCAGG TGCTGGAGGA CGCAGACAGC AGGACCTGCA CTCAGACACC CACCCTTCTG
    CCTTTCTGCA TGAATATTGG CTACGTGAAC CAGGTTTTTG GGGGAGGACT GAATTGTTTG
    GCTTTGGCCG ATCAGAATGC AATGGGTTTT ATATCAACAA TCCATGAGCT TGCATCGGCA
    GAGAGGAAAT TCTACTGCAG CCTCAGTAAA CTGAAGAACC AGATCCTGAG CCCTCTGTTA
    GCCCGTGCAA GTTTGGCCTT GTCTCTGGGT GAGTCGTCCA TGTTGCTCTT TCAAGAAATG
    GCTGGTAGTT TCAGCAAGCT TTGCCATCTG ACAGGGCAAC ACTCAGTGTC CCTCACCAAC
    TTCCTGCAGC AGGGCAAAGT GATCGAAACG CTGGTCTTGA TCAAACACAC AGCCATTTTT
    ATGGATACCT ATAAAGAGTA CAGTGGTCCG GTTGGAAACT TTCTTGTTAT GGGAGGCTTC
    CAGTCATTGC TGAAACCCTC ACAAGAATGC TTTGGGAAGA AACTGGAGTT GATACAGGCT
    CTACTAGAAT CCAAGGAGAA GAACCCGTCG CTCTATGATC TGTTAGTAAA CTTGTTTTAC
    ATGCCCTTGC AACACTTGAA TGGGTATAAC AGGTTACTTC TGAAACTAGC CAACTGTTTT
    GAAGTGAACA CTGATGAATA CCAGAGGATT CAAAATGGCT GCTCCAAATA TGAGGCTTTA
    GCTCGCCTTG TCAGCAAGCA GAGAAAAGAT GCTGAGGCCA CTTTCCATTT CTGGAAGAAC
    TTCAAAGGAA AGAATTTGGA AGCTCTGCGT AAGCCGTCCC GTCGTCTGGT ATGTGAGAGC
    AGTAATAAAG GCCTAACGTT ACAGAACGCT GGAAGATTTT CAGCCAGCTG GTTCATTCTC
    TTCAATGACG TGCTGGTTCA TGCACAGGGA AGCATTCCAT CAAAGAAGCT TTTCTCATCT
    CATTATGTCT ATCCACTTGC TACACTCTGG GTGGAGCCCA TCTCAGATGA AAGCTTGGGA
    TTACTTGGCC TTAAGCTGAC CACACCTGAA GATAGCTTTG TTGTGCTGGC AACGTCCCCT
    CTGGAAAAGG GCAAGTGGCT GAGAGCCATT AACCAGGCCA TTGATGAAGT GCTGGTGTCA
    GGCATGAACG GGAGGACGCG TGTTTCTAGA AGCAGCCAGA AAGGTGAACC TCCTGTGTCC
    CGTACTTCCA ACTACACCTT CAGCAAAGAG GGCCGACTTA AAGATGCCAA ATATGAAGGC
    CGCTGGCTGT CAGGCAAACC TCACGGCAAA GGGAATTTGA AGTGGCCTGA TGGGACAATG
    TACTGTGGCA CATTCAAGAG TGGACTGGAA GATGGGTTTG GAGATTTTAT GACGCCCAGT
    ACCACCTTCA ACAAGTTTGA GCGTTACCAA GGTCACTGGA AGGAAGGAAA AATGCATGGC
    TTTGGCACTT TCTGGTATGC TTCAGGTGAA GTCTATGAGG GCTCTTTCAG AGAGAACATG
    CGTCATGGTC ACGGCATGTT ACGCAGTGGG AAAGTGGCCT CTCCCTCGTC CAGTAGTGTG
    TTTGTGGGTC AGTGGGTTCA AGGGAAGAGG ACAGGCTATG GGGTGTGTGA TGACTTCAGC
    AGAGGAGAGA AGTACATGGG GATATGGCAT GATGACCAGC GTCACGGAGA CGGGGTTGTT
    ATCACACAAT TCGGATTGTA TTATGAAGGA AACTTCTGTA ATAACAGAAT GATGGGCAAT
    GGGACTCTTC TTTGTGATGA CGACACAGTC TTTGAGGGTG ACTTTCAGGA TGACTGGACA
    CTGTGTGGAA AGGGAATTCT GTTCATGCCT AATGGAGACT CTTTTGATGG TGTCTTTGAC
    GGCCACTGGG GGTCTGGACT GAGAGTGGCT GGAACTTTTA CAAAACCCTT TGAAATAGCA
    AGGAGAGATA TCAAGATGCC TGTGGCTGGT CAACATGCAG TTCCTGCAGA AGAAAAGTGG
    AAAGCCGTCT TTGAGGAATG CTGGAGCCGA CTGGGCTGTG AAACCGCTGG TCAGGGGGCA
    TTGGAGAAAG CCTGGGAGAA CATCGCCATC TCCCTCACTG CCAAACGCAG ACAACAGAGA
    GATCGTTCAG AAGAGTTGAG CCGATCACAA ACTAAGATGC TGGAGTGTCT TGAGGTGATT
    CCTCTGCATG AGGGCCCTGT TACTGCAAAT TCCTACAACA ACATCCGTCG GTATCTTGTA
    AAGGCTTGTG ATACCCCTCT GCACCCACTG GGCTGGCTGC TGGAGACCCT TGTCACCGTG
    TACAGGATGA CTTATGTTGG AGTGGGCTCT AGCAGACGCC TCCTGCAGCA GGCAGTGGAG
    GAGATCCAGT CATACCTAAC ACGCATTTTC CACATAGTCA GGTTTCTGTT TCCTGGTCTC
    CCTGAGGATG GAGGTTTTAT TGTGGACTCG TCTTGTTCCT CAGATGAGGG CACAGACTCT
    CTGAACAGCA GTACTCATTC AGAAGCTCCA CAGCAGGGTT GGACCGTGAG CAGCTCAAGT
    CTCCTCCTGC CGGTGCTACT GCCGAGGCTC TACCCTCCAC TCTTCACCCT CTATACTTTG
    GAGAAAGAGA GAGAGGAGGA GCTGTACTGG AACTGTGTGT TAAGACTCAA CAAACAGCCT
    GATCTAGCCC TTCTTGCATT CCTTGGGGTG CAACAAAAAT TCTGGCCGGT TTCTATCTCT
    GTATTGGGGG AAATAAAGAA GGTTCTGCCT AGTACTAAAG ACGCCTGCTT TTCCACTGCT
    GTTGAAACCC TGCAACAGAT CAGCACAGCA TTCACTCCTT CAGACAAGTT GCAGGTGATA
    CAGCTGACTT TTGAGGAGGT CACCCAGGAC GTGCAGGCTA TTCTTTGCCA GGACTTTTTG
    TGGTGCATGG ACGACCTCTT TCCAATTTTC CTCTATGTGG TGCTGCGTGC ACGGATCAGA
    AACCTGGGCT CTGAAGTGAG TCTGATAGAA GACCTCACAG ACAGCAATCT ACAGCTGGGA
    CAACTGGGAT TCATGCTGAC AACTCTCAAA GCCTGTTACA ACCAGATTCA GCTTGAGAAG
    ACTACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa14960
    Clone ID Related Accession (Same CDS sequence) XM_679972.8 , XM_021478854.1 , XM_679972.7
    Accession Version XM_021478854.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4989bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product alsin
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTGATGG AGGAACAGGG CAGGTCTGGA GAACAGGATG GCTCTAGAGA GAAAGGTGTG 
    CTCTACAGAT GGCAGGGCTA TTTCTGCAGT GTGAGCCCGG AGAAGCTGCT CCTGTCGAGG
    CCAGTACTCC ATGCTGCATT GGGGTCTTTA CATGGACTTC TGCTGATAGA AGGTGGCCAA
    GTATATAGTT TTGGTGAGCA GCCGTGGAAG CCAGTTGAAC CTCCGCCTGC CAGTCTTGTT
    TTAGAGAGCA CCCTGAGTGG TCAGCATGTG ATCTCTGTGT CTGCGGGCAG CTATCACTGC
    TCTGCTGTAA CAGAGGATGG TCTGGTCCTC ATGTGGGGCG AGAACTCATA CGGCCAGTGT
    GGAGTCTCTG GAACAGACCG GGTCCCCAGT CCCACACCCG TTACTGTGGT GGATGACGAG
    ACTCATCCTC CACAACTGGT CCGAGTCCTA AATGTGGCTT GCGGAGCACA GCACACTCTG
    GCTCTGTCAA ACAAGCATGA GGTGTGGGCC TGGGGCAGCG GTTGCCAGCT AGGACTGGTC
    ACCAATGTAT TCCCAGTCTG GAAACCCCAA AAGGTGGAGC ATCTAGTGGG AAGATATGTA
    GTTCAGATTG CCTGTGGAGC GTTTCACAGC CTGGCTTTGG TTCAGTTTCT GCCTTCCCCA
    GATGCAAGCC AGCAAGCTCA GGATAAATGT GGGCAGTGCA ATCAGATCCG TTACACCATG
    ACTGACAAGG AAGACCATGT TGTTATTTCC GATGAGCACT ACTGCCCACT TGGAGTGGAA
    CTGGCAGAAT CTAAAGAAGG TCGAAAGTCA GGACAGAGTT CTCCAGCTCA AACACCACAA
    AGCAAAAACT CATCGCCGAA TTACCAAGTG TCACCTCTAA CCTCAACGGA CACCTTTTCA
    TCTTCTTTTC AGCAAAATAC CACAAACCAG GAGACACAAC CAGACCCTTC TCTATCTGAG
    ACTGGACCTG CATGTCTTCA GCGATCCAAA AGCACTCCCT ACCCTGATGA GCAGGCTCTG
    AAAGACTACC TCAAAAAAAT TTTAGACCAG ACTCTTGCTG AGCAGGCAAC ATCAGCAGGA
    GGTTCCCATC CTCCGAGCCG TCAGTCATCA CTAACATGTC CTTTCGGATC AGAGCAGAGT
    GATGAACTTA CACTACGTCC CTCTTCACCA GCACTGAGCA GGACTGACAG CATCACATCC
    TCTGCCAGAA AACACATCAG TGCTTCAGAT ACTGTTACAG AGCAGAAAAA CCAACATATA
    ATACCAACGC TGGACTCCAC TTCTGATGAT TCTTCTCAGC ACTCCAGTGT GGATGGAGGC
    ATTTATCTCA GTGACCTCGT GATTAGCAAT GGGTGTTTGT TTGAATCTTG TGAGGAGGGT
    GGAGATACTG CTGGGACTGA CTTCTGTCCA GCTGATCTGC TTCAAAGCAA GAAAAGCATC
    AGTCTGACGG ATGTTACCTT GGAGGACACT GATCGCAATA GCTTCTCTCC AGTCTCGCCT
    TCTGACACTC ACCGAACCAG CAATTTGAGA GCAGTGAGAG CTTCTACCTC AGAGTCCAGA
    AATCTCCTTC CTTCCCTCTT CACGGAGGTG TGGAGTTGGG GGAAAGGTCA CGAAGGTCAG
    CTTGGTCATG GAGACTATTT GCCCAGGCTA CAGCCTCTCT GCATCAAGAG CTTAAGCAAA
    AAGGAAGTTG TCAAGATCAC AGCTGGAGCA AATCACTCCT TGGCGCTGAC AGCTCAATGT
    CAGGTTTATT CATGGGGAAG TGAAAAATTT GGCCAGCTGG GTCGTATGAA TTCTCCCAGC
    ACAGTTCCTA ATCTCACCAC GGTGTCAGAT GGTATTCGCG TCTGGGATGT GGCAGCAGGA
    CAAACGCACA CTTTGCTATT GGCCGATGGA GACTGTTATC AGCCCATACT GTACTACAGC
    GGAGAGCAGG TGCTGGAGGA CGCAGACAGC AGGACCTGCA CTCAGACACC CACCCTTCTG
    CCTTTCTGCA TGAATATTGG CTACGTGAAC CAGGTTTTTG GGGGAGGACT GAATTGTTTG
    GCTTTGGCCG ATCAGAATGC AATGGGTTTT ATATCAACAA TCCATGAGCT TGCATCGGCA
    GAGAGGAAAT TCTACTGCAG CCTCAGTAAA CTGAAGAACC AGATCCTGAG CCCTCTGTTA
    GCCCGTGCAA GTTTGGCCTT GTCTCTGGGT GAGTCGTCCA TGTTGCTCTT TCAAGAAATG
    GCTGGTAGTT TCAGCAAGCT TTGCCATCTG ACAGGGCAAC ACTCAGTGTC CCTCACCAAC
    TTCCTGCAGC AGGGCAAAGT GATCGAAACG CTGGTCTTGA TCAAACACAC AGCCATTTTT
    ATGGATACCT ATAAAGAGTA CAGTGGTCCG GTTGGAAACT TTCTTGTTAT GGGAGGCTTC
    CAGTCATTGC TGAAACCCTC ACAAGAATGC TTTGGGAAGA AACTGGAGTT GATACAGGCT
    CTACTAGAAT CCAAGGAGAA GAACCCGTCG CTCTATGATC TGTTAGTAAA CTTGTTTTAC
    ATGCCCTTGC AACACTTGAA TGGGTATAAC AGGTTACTTC TGAAACTAGC CAACTGTTTT
    GAAGTGAACA CTGATGAATA CCAGAGGATT CAAAATGGCT GCTCCAAATA TGAGGCTTTA
    GCTCGCCTTG TCAGCAAGCA GAGAAAAGAT GCTGAGGCCA CTTTCCATTT CTGGAAGAAC
    TTCAAAGGAA AGAATTTGGA AGCTCTGCGT AAGCCGTCCC GTCGTCTGGT ATGTGAGAGC
    AGTAATAAAG GCCTAACGTT ACAGAACGCT GGAAGATTTT CAGCCAGCTG GTTCATTCTC
    TTCAATGACG TGCTGGTTCA TGCACAGGGA AGCATTCCAT CAAAGAAGCT TTTCTCATCT
    CATTATGTCT ATCCACTTGC TACACTCTGG GTGGAGCCCA TCTCAGATGA AAGCTTGGGA
    TTACTTGGCC TTAAGCTGAC CACACCTGAA GATAGCTTTG TTGTGCTGGC AACGTCCCCT
    CTGGAAAAGG GCAAGTGGCT GAGAGCCATT AACCAGGCCA TTGATGAAGT GCTGGTGTCA
    GGCATGAACG GGAGGACGCG TGTTTCTAGA AGCAGCCAGA AAGGTGAACC TCCTGTGTCC
    CGTACTTCCA ACTACACCTT CAGCAAAGAG GGCCGACTTA AAGATGCCAA ATATGAAGGC
    CGCTGGCTGT CAGGCAAACC TCACGGCAAA GGGAATTTGA AGTGGCCTGA TGGGACAATG
    TACTGTGGCA CATTCAAGAG TGGACTGGAA GATGGGTTTG GAGATTTTAT GACGCCCAGT
    ACCACCTTCA ACAAGTTTGA GCGTTACCAA GGTCACTGGA AGGAAGGAAA AATGCATGGC
    TTTGGCACTT TCTGGTATGC TTCAGGTGAA GTCTATGAGG GCTCTTTCAG AGAGAACATG
    CGTCATGGTC ACGGCATGTT ACGCAGTGGG AAAGTGGCCT CTCCCTCGTC CAGTAGTGTG
    TTTGTGGGTC AGTGGGTTCA AGGGAAGAGG ACAGGCTATG GGGTGTGTGA TGACTTCAGC
    AGAGGAGAGA AGTACATGGG GATATGGCAT GATGACCAGC GTCACGGAGA CGGGGTTGTT
    ATCACACAAT TCGGATTGTA TTATGAAGGA AACTTCTGTA ATAACAGAAT GATGGGCAAT
    GGGACTCTTC TTTGTGATGA CGACACAGTC TTTGAGGGTG ACTTTCAGGA TGACTGGACA
    CTGTGTGGAA AGGGAATTCT GTTCATGCCT AATGGAGACT CTTTTGATGG TGTCTTTGAC
    GGCCACTGGG GGTCTGGACT GAGAGTGGCT GGAACTTTTA CAAAACCCTT TGAAATAGCA
    AGGAGAGATA TCAAGATGCC TGTGGCTGGT CAACATGCAG TTCCTGCAGA AGAAAAGTGG
    AAAGCCGTCT TTGAGGAATG CTGGAGCCGA CTGGGCTGTG AAACCGCTGG TCAGGGGGCA
    TTGGAGAAAG CCTGGGAGAA CATCGCCATC TCCCTCACTG CCAAACGCAG ACAACAGAGA
    GATCGTTCAG AAGAGTTGAG CCGATCACAA ACTAAGATGC TGGAGTGTCT TGAGGTGATT
    CCTCTGCATG AGGGCCCTGT TACTGCAAAT TCCTACAACA ACATCCGTCG GTATCTTGTA
    AAGGCTTGTG ATACCCCTCT GCACCCACTG GGCTGGCTGC TGGAGACCCT TGTCACCGTG
    TACAGGATGA CTTATGTTGG AGTGGGCTCT AGCAGACGCC TCCTGCAGCA GGCAGTGGAG
    GAGATCCAGT CATACCTAAC ACGCATTTTC CACATAGTCA GGTTTCTGTT TCCTGGTCTC
    CCTGAGGATG GAGGTTTTAT TGTGGACTCG TCTTGTTCCT CAGATGAGGG CACAGACTCT
    CTGAACAGCA GTACTCATTC AGAAGCTCCA CAGCAGGGTT GGACCGTGAG CAGCTCAAGT
    CTCCTCCTGC CGGTGCTACT GCCGAGGCTC TACCCTCCAC TCTTCACCCT CTATACTTTG
    GAGAAAGAGA GAGAGGAGGA GCTGTACTGG AACTGTGTGT TAAGACTCAA CAAACAGCCT
    GATCTAGCCC TTCTTGCATT CCTTGGGGTG CAACAAAAAT TCTGGCCGGT TTCTATCTCT
    GTATTGGGGG AAATAAAGAA GGTTCTGCCT AGTACTAAAG ACGCCTGCTT TTCCACTGCT
    GTTGAAACCC TGCAACAGAT CAGCACAGCA TTCACTCCTT CAGACAAGTT GCAGGTGATA
    CAGCTGACTT TTGAGGAGGT CACCCAGGAC GTGCAGGCTA TTCTTTGCCA GGACTTTTTG
    TGGTGCATGG ACGACCTCTT TCCAATTTTC CTCTATGTGG TGCTGCGTGC ACGGATCAGA
    AACCTGGGCT CTGAAGTGAG TCTGATAGAA GACCTCACAG ACAGCAATCT ACAGCTGGGA
    CAACTGGGAT TCATGCTGAC AACTCTCAAA GCCTGTTACA ACCAGATTCA GCTTGAGAAG
    ACTACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021334529.1
    CDS135..5123
    Translation

    Target ORF information:

    RefSeq Version XM_021478854.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio amyotrophic lateral sclerosis 2a (juvenile) (als2a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021478854.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTGATGG AGGAACAGGG CAGGTCTGGA GAACAGGATG GCTCTAGAGA GAAAGGTGTG 
    CTCTACAGAT GGCAGGGCTA TTTCTGCAGT GTGAGCCCGG AGAAGCTGCT CCTGTCGAGG
    CCAGTACTCC ATGCTGCATT GGGGTCTTTA CATGGACTTC TGCTGATAGA AGGTGGCCAA
    GTATATAGTT TTGGTGAGCA GCCGTGGAAG CCAGTTGAAC CTCCGCCTGC CAGTCTTGTT
    TTAGAGAGCA CCCTGAGTGG TCAGCATGTG ATCTCTGTGT CTGCGGGCAG CTATCACTGC
    TCTGCTGTAA CAGAGGATGG TCTGGTCCTC ATGTGGGGCG AGAACTCATA CGGCCAGTGT
    GGAGTCTCTG GAACAGACCG GGTCCCCAGT CCCACACCCG TTACTGTGGT GGATGACGAG
    ACTCATCCTC CACAACTGGT CCGAGTCCTA AATGTGGCTT GCGGAGCACA GCACACTCTG
    GCTCTGTCAA ACAAGCATGA GGTGTGGGCC TGGGGCAGCG GTTGCCAGCT AGGACTGGTC
    ACCAATGTAT TCCCAGTCTG GAAACCCCAA AAGGTGGAGC ATCTAGTGGG AAGATATGTA
    GTTCAGATTG CCTGTGGAGC GTTTCACAGC CTGGCTTTGG TTCAGTTTCT GCCTTCCCCA
    GATGCAAGCC AGCAAGCTCA GGATAAATGT GGGCAGTGCA ATCAGATCCG TTACACCATG
    ACTGACAAGG AAGACCATGT TGTTATTTCC GATGAGCACT ACTGCCCACT TGGAGTGGAA
    CTGGCAGAAT CTAAAGAAGG TCGAAAGTCA GGACAGAGTT CTCCAGCTCA AACACCACAA
    AGCAAAAACT CATCGCCGAA TTACCAAGTG TCACCTCTAA CCTCAACGGA CACCTTTTCA
    TCTTCTTTTC AGCAAAATAC CACAAACCAG GAGACACAAC CAGACCCTTC TCTATCTGAG
    ACTGGACCTG CATGTCTTCA GCGATCCAAA AGCACTCCCT ACCCTGATGA GCAGGCTCTG
    AAAGACTACC TCAAAAAAAT TTTAGACCAG ACTCTTGCTG AGCAGGCAAC ATCAGCAGGA
    GGTTCCCATC CTCCGAGCCG TCAGTCATCA CTAACATGTC CTTTCGGATC AGAGCAGAGT
    GATGAACTTA CACTACGTCC CTCTTCACCA GCACTGAGCA GGACTGACAG CATCACATCC
    TCTGCCAGAA AACACATCAG TGCTTCAGAT ACTGTTACAG AGCAGAAAAA CCAACATATA
    ATACCAACGC TGGACTCCAC TTCTGATGAT TCTTCTCAGC ACTCCAGTGT GGATGGAGGC
    ATTTATCTCA GTGACCTCGT GATTAGCAAT GGGTGTTTGT TTGAATCTTG TGAGGAGGGT
    GGAGATACTG CTGGGACTGA CTTCTGTCCA GCTGATCTGC TTCAAAGCAA GAAAAGCATC
    AGTCTGACGG ATGTTACCTT GGAGGACACT GATCGCAATA GCTTCTCTCC AGTCTCGCCT
    TCTGACACTC ACCGAACCAG CAATTTGAGA GCAGTGAGAG CTTCTACCTC AGAGTCCAGA
    AATCTCCTTC CTTCCCTCTT CACGGAGGTG TGGAGTTGGG GGAAAGGTCA CGAAGGTCAG
    CTTGGTCATG GAGACTATTT GCCCAGGCTA CAGCCTCTCT GCATCAAGAG CTTAAGCAAA
    AAGGAAGTTG TCAAGATCAC AGCTGGAGCA AATCACTCCT TGGCGCTGAC AGCTCAATGT
    CAGGTTTATT CATGGGGAAG TGAAAAATTT GGCCAGCTGG GTCGTATGAA TTCTCCCAGC
    ACAGTTCCTA ATCTCACCAC GGTGTCAGAT GGTATTCGCG TCTGGGATGT GGCAGCAGGA
    CAAACGCACA CTTTGCTATT GGCCGATGGA GACTGTTATC AGCCCATACT GTACTACAGC
    GGAGAGCAGG TGCTGGAGGA CGCAGACAGC AGGACCTGCA CTCAGACACC CACCCTTCTG
    CCTTTCTGCA TGAATATTGG CTACGTGAAC CAGGTTTTTG GGGGAGGACT GAATTGTTTG
    GCTTTGGCCG ATCAGAATGC AATGGGTTTT ATATCAACAA TCCATGAGCT TGCATCGGCA
    GAGAGGAAAT TCTACTGCAG CCTCAGTAAA CTGAAGAACC AGATCCTGAG CCCTCTGTTA
    GCCCGTGCAA GTTTGGCCTT GTCTCTGGGT GAGTCGTCCA TGTTGCTCTT TCAAGAAATG
    GCTGGTAGTT TCAGCAAGCT TTGCCATCTG ACAGGGCAAC ACTCAGTGTC CCTCACCAAC
    TTCCTGCAGC AGGGCAAAGT GATCGAAACG CTGGTCTTGA TCAAACACAC AGCCATTTTT
    ATGGATACCT ATAAAGAGTA CAGTGGTCCG GTTGGAAACT TTCTTGTTAT GGGAGGCTTC
    CAGTCATTGC TGAAACCCTC ACAAGAATGC TTTGGGAAGA AACTGGAGTT GATACAGGCT
    CTACTAGAAT CCAAGGAGAA GAACCCGTCG CTCTATGATC TGTTAGTAAA CTTGTTTTAC
    ATGCCCTTGC AACACTTGAA TGGGTATAAC AGGTTACTTC TGAAACTAGC CAACTGTTTT
    GAAGTGAACA CTGATGAATA CCAGAGGATT CAAAATGGCT GCTCCAAATA TGAGGCTTTA
    GCTCGCCTTG TCAGCAAGCA GAGAAAAGAT GCTGAGGCCA CTTTCCATTT CTGGAAGAAC
    TTCAAAGGAA AGAATTTGGA AGCTCTGCGT AAGCCGTCCC GTCGTCTGGT ATGTGAGAGC
    AGTAATAAAG GCCTAACGTT ACAGAACGCT GGAAGATTTT CAGCCAGCTG GTTCATTCTC
    TTCAATGACG TGCTGGTTCA TGCACAGGGA AGCATTCCAT CAAAGAAGCT TTTCTCATCT
    CATTATGTCT ATCCACTTGC TACACTCTGG GTGGAGCCCA TCTCAGATGA AAGCTTGGGA
    TTACTTGGCC TTAAGCTGAC CACACCTGAA GATAGCTTTG TTGTGCTGGC AACGTCCCCT
    CTGGAAAAGG GCAAGTGGCT GAGAGCCATT AACCAGGCCA TTGATGAAGT GCTGGTGTCA
    GGCATGAACG GGAGGACGCG TGTTTCTAGA AGCAGCCAGA AAGGTGAACC TCCTGTGTCC
    CGTACTTCCA ACTACACCTT CAGCAAAGAG GGCCGACTTA AAGATGCCAA ATATGAAGGC
    CGCTGGCTGT CAGGCAAACC TCACGGCAAA GGGAATTTGA AGTGGCCTGA TGGGACAATG
    TACTGTGGCA CATTCAAGAG TGGACTGGAA GATGGGTTTG GAGATTTTAT GACGCCCAGT
    ACCACCTTCA ACAAGTTTGA GCGTTACCAA GGTCACTGGA AGGAAGGAAA AATGCATGGC
    TTTGGCACTT TCTGGTATGC TTCAGGTGAA GTCTATGAGG GCTCTTTCAG AGAGAACATG
    CGTCATGGTC ACGGCATGTT ACGCAGTGGG AAAGTGGCCT CTCCCTCGTC CAGTAGTGTG
    TTTGTGGGTC AGTGGGTTCA AGGGAAGAGG ACAGGCTATG GGGTGTGTGA TGACTTCAGC
    AGAGGAGAGA AGTACATGGG GATATGGCAT GATGACCAGC GTCACGGAGA CGGGGTTGTT
    ATCACACAAT TCGGATTGTA TTATGAAGGA AACTTCTGTA ATAACAGAAT GATGGGCAAT
    GGGACTCTTC TTTGTGATGA CGACACAGTC TTTGAGGGTG ACTTTCAGGA TGACTGGACA
    CTGTGTGGAA AGGGAATTCT GTTCATGCCT AATGGAGACT CTTTTGATGG TGTCTTTGAC
    GGCCACTGGG GGTCTGGACT GAGAGTGGCT GGAACTTTTA CAAAACCCTT TGAAATAGCA
    AGGAGAGATA TCAAGATGCC TGTGGCTGGT CAACATGCAG TTCCTGCAGA AGAAAAGTGG
    AAAGCCGTCT TTGAGGAATG CTGGAGCCGA CTGGGCTGTG AAACCGCTGG TCAGGGGGCA
    TTGGAGAAAG CCTGGGAGAA CATCGCCATC TCCCTCACTG CCAAACGCAG ACAACAGAGA
    GATCGTTCAG AAGAGTTGAG CCGATCACAA ACTAAGATGC TGGAGTGTCT TGAGGTGATT
    CCTCTGCATG AGGGCCCTGT TACTGCAAAT TCCTACAACA ACATCCGTCG GTATCTTGTA
    AAGGCTTGTG ATACCCCTCT GCACCCACTG GGCTGGCTGC TGGAGACCCT TGTCACCGTG
    TACAGGATGA CTTATGTTGG AGTGGGCTCT AGCAGACGCC TCCTGCAGCA GGCAGTGGAG
    GAGATCCAGT CATACCTAAC ACGCATTTTC CACATAGTCA GGTTTCTGTT TCCTGGTCTC
    CCTGAGGATG GAGGTTTTAT TGTGGACTCG TCTTGTTCCT CAGATGAGGG CACAGACTCT
    CTGAACAGCA GTACTCATTC AGAAGCTCCA CAGCAGGGTT GGACCGTGAG CAGCTCAAGT
    CTCCTCCTGC CGGTGCTACT GCCGAGGCTC TACCCTCCAC TCTTCACCCT CTATACTTTG
    GAGAAAGAGA GAGAGGAGGA GCTGTACTGG AACTGTGTGT TAAGACTCAA CAAACAGCCT
    GATCTAGCCC TTCTTGCATT CCTTGGGGTG CAACAAAAAT TCTGGCCGGT TTCTATCTCT
    GTATTGGGGG AAATAAAGAA GGTTCTGCCT AGTACTAAAG ACGCCTGCTT TTCCACTGCT
    GTTGAAACCC TGCAACAGAT CAGCACAGCA TTCACTCCTT CAGACAAGTT GCAGGTGATA
    CAGCTGACTT TTGAGGAGGT CACCCAGGAC GTGCAGGCTA TTCTTTGCCA GGACTTTTTG
    TGGTGCATGG ACGACCTCTT TCCAATTTTC CTCTATGTGG TGCTGCGTGC ACGGATCAGA
    AACCTGGGCT CTGAAGTGAG TCTGATAGAA GACCTCACAG ACAGCAATCT ACAGCTGGGA
    CAACTGGGAT TCATGCTGAC AACTCTCAAA GCCTGTTACA ACCAGATTCA GCTTGAGAAG
    ACTACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa14960
    Clone ID Related Accession (Same CDS sequence) XM_679972.8 , XM_021478854.1 , XM_679972.7
    Accession Version XM_679972.7 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4989bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product alsin
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_679972.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTGATGG AGGAACAGGG CAGGTCTGGA GAACAGGATG GCTCTAGAGA GAAAGGTGTG 
    CTCTACAGAT GGCAGGGCTA TTTCTGCAGT GTGAGCCCGG AGAAGCTGCT CCTGTCGAGG
    CCAGTACTCC ATGCTGCATT GGGGTCTTTA CATGGACTTC TGCTGATAGA AGGTGGCCAA
    GTATATAGTT TTGGTGAGCA GCCGTGGAAG CCAGTTGAAC CTCCGCCTGC CAGTCTTGTT
    TTAGAGAGCA CCCTGAGTGG TCAGCATGTG ATCTCTGTGT CTGCGGGCAG CTATCACTGC
    TCTGCTGTAA CAGAGGATGG TCTGGTCCTC ATGTGGGGCG AGAACTCATA CGGCCAGTGT
    GGAGTCTCTG GAACAGACCG GGTCCCCAGT CCCACACCCG TTACTGTGGT GGATGACGAG
    ACTCATCCTC CACAACTGGT CCGAGTCCTA AATGTGGCTT GCGGAGCACA GCACACTCTG
    GCTCTGTCAA ACAAGCATGA GGTGTGGGCC TGGGGCAGCG GTTGCCAGCT AGGACTGGTC
    ACCAATGTAT TCCCAGTCTG GAAACCCCAA AAGGTGGAGC ATCTAGTGGG AAGATATGTA
    GTTCAGATTG CCTGTGGAGC GTTTCACAGC CTGGCTTTGG TTCAGTTTCT GCCTTCCCCA
    GATGCAAGCC AGCAAGCTCA GGATAAATGT GGGCAGTGCA ATCAGATCCG TTACACCATG
    ACTGACAAGG AAGACCATGT TGTTATTTCC GATGAGCACT ACTGCCCACT TGGAGTGGAA
    CTGGCAGAAT CTAAAGAAGG TCGAAAGTCA GGACAGAGTT CTCCAGCTCA AACACCACAA
    AGCAAAAACT CATCGCCGAA TTACCAAGTG TCACCTCTAA CCTCAACGGA CACCTTTTCA
    TCTTCTTTTC AGCAAAATAC CACAAACCAG GAGACACAAC CAGACCCTTC TCTATCTGAG
    ACTGGACCTG CATGTCTTCA GCGATCCAAA AGCACTCCCT ACCCTGATGA GCAGGCTCTG
    AAAGACTACC TCAAAAAAAT TTTAGACCAG ACTCTTGCTG AGCAGGCAAC ATCAGCAGGA
    GGTTCCCATC CTCCGAGCCG TCAGTCATCA CTAACATGTC CTTTCGGATC AGAGCAGAGT
    GATGAACTTA CACTACGTCC CTCTTCACCA GCACTGAGCA GGACTGACAG CATCACATCC
    TCTGCCAGAA AACACATCAG TGCTTCAGAT ACTGTTACAG AGCAGAAAAA CCAACATATA
    ATACCAACGC TGGACTCCAC TTCTGATGAT TCTTCTCAGC ACTCCAGTGT GGATGGAGGC
    ATTTATCTCA GTGACCTCGT GATTAGCAAT GGGTGTTTGT TTGAATCTTG TGAGGAGGGT
    GGAGATACTG CTGGGACTGA CTTCTGTCCA GCTGATCTGC TTCAAAGCAA GAAAAGCATC
    AGTCTGACGG ATGTTACCTT GGAGGACACT GATCGCAATA GCTTCTCTCC AGTCTCGCCT
    TCTGACACTC ACCGAACCAG CAATTTGAGA GCAGTGAGAG CTTCTACCTC AGAGTCCAGA
    AATCTCCTTC CTTCCCTCTT CACGGAGGTG TGGAGTTGGG GGAAAGGTCA CGAAGGTCAG
    CTTGGTCATG GAGACTATTT GCCCAGGCTA CAGCCTCTCT GCATCAAGAG CTTAAGCAAA
    AAGGAAGTTG TCAAGATCAC AGCTGGAGCA AATCACTCCT TGGCGCTGAC AGCTCAATGT
    CAGGTTTATT CATGGGGAAG TGAAAAATTT GGCCAGCTGG GTCGTATGAA TTCTCCCAGC
    ACAGTTCCTA ATCTCACCAC GGTGTCAGAT GGTATTCGCG TCTGGGATGT GGCAGCAGGA
    CAAACGCACA CTTTGCTATT GGCCGATGGA GACTGTTATC AGCCCATACT GTACTACAGC
    GGAGAGCAGG TGCTGGAGGA CGCAGACAGC AGGACCTGCA CTCAGACACC CACCCTTCTG
    CCTTTCTGCA TGAATATTGG CTACGTGAAC CAGGTTTTTG GGGGAGGACT GAATTGTTTG
    GCTTTGGCCG ATCAGAATGC AATGGGTTTT ATATCAACAA TCCATGAGCT TGCATCGGCA
    GAGAGGAAAT TCTACTGCAG CCTCAGTAAA CTGAAGAACC AGATCCTGAG CCCTCTGTTA
    GCCCGTGCAA GTTTGGCCTT GTCTCTGGGT GAGTCGTCCA TGTTGCTCTT TCAAGAAATG
    GCTGGTAGTT TCAGCAAGCT TTGCCATCTG ACAGGGCAAC ACTCAGTGTC CCTCACCAAC
    TTCCTGCAGC AGGGCAAAGT GATCGAAACG CTGGTCTTGA TCAAACACAC AGCCATTTTT
    ATGGATACCT ATAAAGAGTA CAGTGGTCCG GTTGGAAACT TTCTTGTTAT GGGAGGCTTC
    CAGTCATTGC TGAAACCCTC ACAAGAATGC TTTGGGAAGA AACTGGAGTT GATACAGGCT
    CTACTAGAAT CCAAGGAGAA GAACCCGTCG CTCTATGATC TGTTAGTAAA CTTGTTTTAC
    ATGCCCTTGC AACACTTGAA TGGGTATAAC AGGTTACTTC TGAAACTAGC CAACTGTTTT
    GAAGTGAACA CTGATGAATA CCAGAGGATT CAAAATGGCT GCTCCAAATA TGAGGCTTTA
    GCTCGCCTTG TCAGCAAGCA GAGAAAAGAT GCTGAGGCCA CTTTCCATTT CTGGAAGAAC
    TTCAAAGGAA AGAATTTGGA AGCTCTGCGT AAGCCGTCCC GTCGTCTGGT ATGTGAGAGC
    AGTAATAAAG GCCTAACGTT ACAGAACGCT GGAAGATTTT CAGCCAGCTG GTTCATTCTC
    TTCAATGACG TGCTGGTTCA TGCACAGGGA AGCATTCCAT CAAAGAAGCT TTTCTCATCT
    CATTATGTCT ATCCACTTGC TACACTCTGG GTGGAGCCCA TCTCAGATGA AAGCTTGGGA
    TTACTTGGCC TTAAGCTGAC CACACCTGAA GATAGCTTTG TTGTGCTGGC AACGTCCCCT
    CTGGAAAAGG GCAAGTGGCT GAGAGCCATT AACCAGGCCA TTGATGAAGT GCTGGTGTCA
    GGCATGAACG GGAGGACGCG TGTTTCTAGA AGCAGCCAGA AAGGTGAACC TCCTGTGTCC
    CGTACTTCCA ACTACACCTT CAGCAAAGAG GGCCGACTTA AAGATGCCAA ATATGAAGGC
    CGCTGGCTGT CAGGCAAACC TCACGGCAAA GGGAATTTGA AGTGGCCTGA TGGGACAATG
    TACTGTGGCA CATTCAAGAG TGGACTGGAA GATGGGTTTG GAGATTTTAT GACGCCCAGT
    ACCACCTTCA ACAAGTTTGA GCGTTACCAA GGTCACTGGA AGGAAGGAAA AATGCATGGC
    TTTGGCACTT TCTGGTATGC TTCAGGTGAA GTCTATGAGG GCTCTTTCAG AGAGAACATG
    CGTCATGGTC ACGGCATGTT ACGCAGTGGG AAAGTGGCCT CTCCCTCGTC CAGTAGTGTG
    TTTGTGGGTC AGTGGGTTCA AGGGAAGAGG ACAGGCTATG GGGTGTGTGA TGACTTCAGC
    AGAGGAGAGA AGTACATGGG GATATGGCAT GATGACCAGC GTCACGGAGA CGGGGTTGTT
    ATCACACAAT TCGGATTGTA TTATGAAGGA AACTTCTGTA ATAACAGAAT GATGGGCAAT
    GGGACTCTTC TTTGTGATGA CGACACAGTC TTTGAGGGTG ACTTTCAGGA TGACTGGACA
    CTGTGTGGAA AGGGAATTCT GTTCATGCCT AATGGAGACT CTTTTGATGG TGTCTTTGAC
    GGCCACTGGG GGTCTGGACT GAGAGTGGCT GGAACTTTTA CAAAACCCTT TGAAATAGCA
    AGGAGAGATA TCAAGATGCC TGTGGCTGGT CAACATGCAG TTCCTGCAGA AGAAAAGTGG
    AAAGCCGTCT TTGAGGAATG CTGGAGCCGA CTGGGCTGTG AAACCGCTGG TCAGGGGGCA
    TTGGAGAAAG CCTGGGAGAA CATCGCCATC TCCCTCACTG CCAAACGCAG ACAACAGAGA
    GATCGTTCAG AAGAGTTGAG CCGATCACAA ACTAAGATGC TGGAGTGTCT TGAGGTGATT
    CCTCTGCATG AGGGCCCTGT TACTGCAAAT TCCTACAACA ACATCCGTCG GTATCTTGTA
    AAGGCTTGTG ATACCCCTCT GCACCCACTG GGCTGGCTGC TGGAGACCCT TGTCACCGTG
    TACAGGATGA CTTATGTTGG AGTGGGCTCT AGCAGACGCC TCCTGCAGCA GGCAGTGGAG
    GAGATCCAGT CATACCTAAC ACGCATTTTC CACATAGTCA GGTTTCTGTT TCCTGGTCTC
    CCTGAGGATG GAGGTTTTAT TGTGGACTCG TCTTGTTCCT CAGATGAGGG CACAGACTCT
    CTGAACAGCA GTACTCATTC AGAAGCTCCA CAGCAGGGTT GGACCGTGAG CAGCTCAAGT
    CTCCTCCTGC CGGTGCTACT GCCGAGGCTC TACCCTCCAC TCTTCACCCT CTATACTTTG
    GAGAAAGAGA GAGAGGAGGA GCTGTACTGG AACTGTGTGT TAAGACTCAA CAAACAGCCT
    GATCTAGCCC TTCTTGCATT CCTTGGGGTG CAACAAAAAT TCTGGCCGGT TTCTATCTCT
    GTATTGGGGG AAATAAAGAA GGTTCTGCCT AGTACTAAAG ACGCCTGCTT TTCCACTGCT
    GTTGAAACCC TGCAACAGAT CAGCACAGCA TTCACTCCTT CAGACAAGTT GCAGGTGATA
    CAGCTGACTT TTGAGGAGGT CACCCAGGAC GTGCAGGCTA TTCTTTGCCA GGACTTTTTG
    TGGTGCATGG ACGACCTCTT TCCAATTTTC CTCTATGTGG TGCTGCGTGC ACGGATCAGA
    AACCTGGGCT CTGAAGTGAG TCTGATAGAA GACCTCACAG ACAGCAATCT ACAGCTGGGA
    CAACTGGGAT TCATGCTGAC AACTCTCAAA GCCTGTTACA ACCAGATTCA GCTTGAGAAG
    ACTACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_685064.6
    CDS129..5117
    Translation

    Target ORF information:

    RefSeq Version XM_679972.7
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio amyotrophic lateral sclerosis 2a (juvenile) (als2a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_679972.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTGATGG AGGAACAGGG CAGGTCTGGA GAACAGGATG GCTCTAGAGA GAAAGGTGTG 
    CTCTACAGAT GGCAGGGCTA TTTCTGCAGT GTGAGCCCGG AGAAGCTGCT CCTGTCGAGG
    CCAGTACTCC ATGCTGCATT GGGGTCTTTA CATGGACTTC TGCTGATAGA AGGTGGCCAA
    GTATATAGTT TTGGTGAGCA GCCGTGGAAG CCAGTTGAAC CTCCGCCTGC CAGTCTTGTT
    TTAGAGAGCA CCCTGAGTGG TCAGCATGTG ATCTCTGTGT CTGCGGGCAG CTATCACTGC
    TCTGCTGTAA CAGAGGATGG TCTGGTCCTC ATGTGGGGCG AGAACTCATA CGGCCAGTGT
    GGAGTCTCTG GAACAGACCG GGTCCCCAGT CCCACACCCG TTACTGTGGT GGATGACGAG
    ACTCATCCTC CACAACTGGT CCGAGTCCTA AATGTGGCTT GCGGAGCACA GCACACTCTG
    GCTCTGTCAA ACAAGCATGA GGTGTGGGCC TGGGGCAGCG GTTGCCAGCT AGGACTGGTC
    ACCAATGTAT TCCCAGTCTG GAAACCCCAA AAGGTGGAGC ATCTAGTGGG AAGATATGTA
    GTTCAGATTG CCTGTGGAGC GTTTCACAGC CTGGCTTTGG TTCAGTTTCT GCCTTCCCCA
    GATGCAAGCC AGCAAGCTCA GGATAAATGT GGGCAGTGCA ATCAGATCCG TTACACCATG
    ACTGACAAGG AAGACCATGT TGTTATTTCC GATGAGCACT ACTGCCCACT TGGAGTGGAA
    CTGGCAGAAT CTAAAGAAGG TCGAAAGTCA GGACAGAGTT CTCCAGCTCA AACACCACAA
    AGCAAAAACT CATCGCCGAA TTACCAAGTG TCACCTCTAA CCTCAACGGA CACCTTTTCA
    TCTTCTTTTC AGCAAAATAC CACAAACCAG GAGACACAAC CAGACCCTTC TCTATCTGAG
    ACTGGACCTG CATGTCTTCA GCGATCCAAA AGCACTCCCT ACCCTGATGA GCAGGCTCTG
    AAAGACTACC TCAAAAAAAT TTTAGACCAG ACTCTTGCTG AGCAGGCAAC ATCAGCAGGA
    GGTTCCCATC CTCCGAGCCG TCAGTCATCA CTAACATGTC CTTTCGGATC AGAGCAGAGT
    GATGAACTTA CACTACGTCC CTCTTCACCA GCACTGAGCA GGACTGACAG CATCACATCC
    TCTGCCAGAA AACACATCAG TGCTTCAGAT ACTGTTACAG AGCAGAAAAA CCAACATATA
    ATACCAACGC TGGACTCCAC TTCTGATGAT TCTTCTCAGC ACTCCAGTGT GGATGGAGGC
    ATTTATCTCA GTGACCTCGT GATTAGCAAT GGGTGTTTGT TTGAATCTTG TGAGGAGGGT
    GGAGATACTG CTGGGACTGA CTTCTGTCCA GCTGATCTGC TTCAAAGCAA GAAAAGCATC
    AGTCTGACGG ATGTTACCTT GGAGGACACT GATCGCAATA GCTTCTCTCC AGTCTCGCCT
    TCTGACACTC ACCGAACCAG CAATTTGAGA GCAGTGAGAG CTTCTACCTC AGAGTCCAGA
    AATCTCCTTC CTTCCCTCTT CACGGAGGTG TGGAGTTGGG GGAAAGGTCA CGAAGGTCAG
    CTTGGTCATG GAGACTATTT GCCCAGGCTA CAGCCTCTCT GCATCAAGAG CTTAAGCAAA
    AAGGAAGTTG TCAAGATCAC AGCTGGAGCA AATCACTCCT TGGCGCTGAC AGCTCAATGT
    CAGGTTTATT CATGGGGAAG TGAAAAATTT GGCCAGCTGG GTCGTATGAA TTCTCCCAGC
    ACAGTTCCTA ATCTCACCAC GGTGTCAGAT GGTATTCGCG TCTGGGATGT GGCAGCAGGA
    CAAACGCACA CTTTGCTATT GGCCGATGGA GACTGTTATC AGCCCATACT GTACTACAGC
    GGAGAGCAGG TGCTGGAGGA CGCAGACAGC AGGACCTGCA CTCAGACACC CACCCTTCTG
    CCTTTCTGCA TGAATATTGG CTACGTGAAC CAGGTTTTTG GGGGAGGACT GAATTGTTTG
    GCTTTGGCCG ATCAGAATGC AATGGGTTTT ATATCAACAA TCCATGAGCT TGCATCGGCA
    GAGAGGAAAT TCTACTGCAG CCTCAGTAAA CTGAAGAACC AGATCCTGAG CCCTCTGTTA
    GCCCGTGCAA GTTTGGCCTT GTCTCTGGGT GAGTCGTCCA TGTTGCTCTT TCAAGAAATG
    GCTGGTAGTT TCAGCAAGCT TTGCCATCTG ACAGGGCAAC ACTCAGTGTC CCTCACCAAC
    TTCCTGCAGC AGGGCAAAGT GATCGAAACG CTGGTCTTGA TCAAACACAC AGCCATTTTT
    ATGGATACCT ATAAAGAGTA CAGTGGTCCG GTTGGAAACT TTCTTGTTAT GGGAGGCTTC
    CAGTCATTGC TGAAACCCTC ACAAGAATGC TTTGGGAAGA AACTGGAGTT GATACAGGCT
    CTACTAGAAT CCAAGGAGAA GAACCCGTCG CTCTATGATC TGTTAGTAAA CTTGTTTTAC
    ATGCCCTTGC AACACTTGAA TGGGTATAAC AGGTTACTTC TGAAACTAGC CAACTGTTTT
    GAAGTGAACA CTGATGAATA CCAGAGGATT CAAAATGGCT GCTCCAAATA TGAGGCTTTA
    GCTCGCCTTG TCAGCAAGCA GAGAAAAGAT GCTGAGGCCA CTTTCCATTT CTGGAAGAAC
    TTCAAAGGAA AGAATTTGGA AGCTCTGCGT AAGCCGTCCC GTCGTCTGGT ATGTGAGAGC
    AGTAATAAAG GCCTAACGTT ACAGAACGCT GGAAGATTTT CAGCCAGCTG GTTCATTCTC
    TTCAATGACG TGCTGGTTCA TGCACAGGGA AGCATTCCAT CAAAGAAGCT TTTCTCATCT
    CATTATGTCT ATCCACTTGC TACACTCTGG GTGGAGCCCA TCTCAGATGA AAGCTTGGGA
    TTACTTGGCC TTAAGCTGAC CACACCTGAA GATAGCTTTG TTGTGCTGGC AACGTCCCCT
    CTGGAAAAGG GCAAGTGGCT GAGAGCCATT AACCAGGCCA TTGATGAAGT GCTGGTGTCA
    GGCATGAACG GGAGGACGCG TGTTTCTAGA AGCAGCCAGA AAGGTGAACC TCCTGTGTCC
    CGTACTTCCA ACTACACCTT CAGCAAAGAG GGCCGACTTA AAGATGCCAA ATATGAAGGC
    CGCTGGCTGT CAGGCAAACC TCACGGCAAA GGGAATTTGA AGTGGCCTGA TGGGACAATG
    TACTGTGGCA CATTCAAGAG TGGACTGGAA GATGGGTTTG GAGATTTTAT GACGCCCAGT
    ACCACCTTCA ACAAGTTTGA GCGTTACCAA GGTCACTGGA AGGAAGGAAA AATGCATGGC
    TTTGGCACTT TCTGGTATGC TTCAGGTGAA GTCTATGAGG GCTCTTTCAG AGAGAACATG
    CGTCATGGTC ACGGCATGTT ACGCAGTGGG AAAGTGGCCT CTCCCTCGTC CAGTAGTGTG
    TTTGTGGGTC AGTGGGTTCA AGGGAAGAGG ACAGGCTATG GGGTGTGTGA TGACTTCAGC
    AGAGGAGAGA AGTACATGGG GATATGGCAT GATGACCAGC GTCACGGAGA CGGGGTTGTT
    ATCACACAAT TCGGATTGTA TTATGAAGGA AACTTCTGTA ATAACAGAAT GATGGGCAAT
    GGGACTCTTC TTTGTGATGA CGACACAGTC TTTGAGGGTG ACTTTCAGGA TGACTGGACA
    CTGTGTGGAA AGGGAATTCT GTTCATGCCT AATGGAGACT CTTTTGATGG TGTCTTTGAC
    GGCCACTGGG GGTCTGGACT GAGAGTGGCT GGAACTTTTA CAAAACCCTT TGAAATAGCA
    AGGAGAGATA TCAAGATGCC TGTGGCTGGT CAACATGCAG TTCCTGCAGA AGAAAAGTGG
    AAAGCCGTCT TTGAGGAATG CTGGAGCCGA CTGGGCTGTG AAACCGCTGG TCAGGGGGCA
    TTGGAGAAAG CCTGGGAGAA CATCGCCATC TCCCTCACTG CCAAACGCAG ACAACAGAGA
    GATCGTTCAG AAGAGTTGAG CCGATCACAA ACTAAGATGC TGGAGTGTCT TGAGGTGATT
    CCTCTGCATG AGGGCCCTGT TACTGCAAAT TCCTACAACA ACATCCGTCG GTATCTTGTA
    AAGGCTTGTG ATACCCCTCT GCACCCACTG GGCTGGCTGC TGGAGACCCT TGTCACCGTG
    TACAGGATGA CTTATGTTGG AGTGGGCTCT AGCAGACGCC TCCTGCAGCA GGCAGTGGAG
    GAGATCCAGT CATACCTAAC ACGCATTTTC CACATAGTCA GGTTTCTGTT TCCTGGTCTC
    CCTGAGGATG GAGGTTTTAT TGTGGACTCG TCTTGTTCCT CAGATGAGGG CACAGACTCT
    CTGAACAGCA GTACTCATTC AGAAGCTCCA CAGCAGGGTT GGACCGTGAG CAGCTCAAGT
    CTCCTCCTGC CGGTGCTACT GCCGAGGCTC TACCCTCCAC TCTTCACCCT CTATACTTTG
    GAGAAAGAGA GAGAGGAGGA GCTGTACTGG AACTGTGTGT TAAGACTCAA CAAACAGCCT
    GATCTAGCCC TTCTTGCATT CCTTGGGGTG CAACAAAAAT TCTGGCCGGT TTCTATCTCT
    GTATTGGGGG AAATAAAGAA GGTTCTGCCT AGTACTAAAG ACGCCTGCTT TTCCACTGCT
    GTTGAAACCC TGCAACAGAT CAGCACAGCA TTCACTCCTT CAGACAAGTT GCAGGTGATA
    CAGCTGACTT TTGAGGAGGT CACCCAGGAC GTGCAGGCTA TTCTTTGCCA GGACTTTTTG
    TGGTGCATGG ACGACCTCTT TCCAATTTTC CTCTATGTGG TGCTGCGTGC ACGGATCAGA
    AACCTGGGCT CTGAAGTGAG TCTGATAGAA GACCTCACAG ACAGCAATCT ACAGCTGGGA
    CAACTGGGAT TCATGCTGAC AACTCTCAAA GCCTGTTACA ACCAGATTCA GCTTGAGAAG
    ACTACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.