KDM6BA gene cDNA ORF clone, Danio rerio(Zebrafish)

mRNA	Protein	Name
XM_005172239.4	XP_005172296.1	lysine-specific demethylase 6B
XM_021477779.1	XP_021333454.1	lysine-specific demethylase 6B
XM_021477780.1	XP_021333455.1	lysine-specific demethylase 6B
XM_009303125.2	XP_009301400.1	lysine-specific demethylase 6B
XM_005172240.3	XP_005172297.1	lysine-specific demethylase 6B
XM_017357011.1	XP_017212500.1	lysine-specific demethylase 6B
XM_005172240.4	XP_005172297.1	lysine-specific demethylase 6B
XM_009303125.3	XP_009301400.1	lysine-specific demethylase 6B
XM_005172239.3	XP_005172296.1	lysine-specific demethylase 6B

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa12856	XM_021477779.1 Latest version!	Danio rerio lysine (K)-specific demethylase 6B, a (kdm6ba), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa12856	XM_005172240.4 Latest version!	Danio rerio lysine (K)-specific demethylase 6B, a (kdm6ba), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa12856	XM_017357011.1 Latest version!	Danio rerio lysine (K)-specific demethylase 6B, a (kdm6ba), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa12856	XM_021477780.1 Latest version!	Danio rerio lysine (K)-specific demethylase 6B, a (kdm6ba), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa12856	XM_005172239.4 Latest version!	Danio rerio lysine (K)-specific demethylase 6B, a (kdm6ba), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa12856	XM_009303125.3 Latest version!	Danio rerio lysine (K)-specific demethylase 6B, a (kdm6ba), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa12856	XM_005172240.3	Danio rerio lysine (K)-specific demethylase 6B, a (kdm6ba), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa12856	XM_009303125.2	Danio rerio lysine (K)-specific demethylase 6B, a (kdm6ba), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa12856	XM_005172239.3	Danio rerio lysine (K)-specific demethylase 6B, a (kdm6ba), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

KDM6BA ( XM_005172239.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005172296.1 Danio rerio lysine (K)-specific demethylase 6B, a (kdm6ba), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa12856
Clone ID Related Accession (Same CDS sequence)	XM_005172239.4 , XM_021477779.1 , XM_021477780.1 , XM_009303125.2 , XM_005172240.3 , XM_017357011.1 , XM_005172240.4 , XM_009303125.3 , XM_005172239.3
Accession Version	XM_005172239.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	lysine-specific demethylase 6B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172239.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCACA CAGCAGAGCA GTTCACCGGG TGCAGCACAC GGGACCCTTT TCCTCTGGAC 
GGACTCAACC GGGGACCATG GGCTCCCGTG GGCAGCCGTG CCTGGCCACC TCCTTCCAGG 
TGCTTAACTG GTGTCAATCA GCACCAGCTT CTTCCCCATA TGCCCAGTCA CATGACTGGG 
CTAAATCATC CTAGTAAATT CTTGAACAAT GGACCCATAC ATCGAGGGGA TAAGCAAGAC 
CAGTCTCAGG CTTTGATGCC GGCATTGCAA AGAATGCCCC CTGCTCCTCC CAGGCCTTGG 
GATCCAACCA GAGCTGGCCA AGGGTATGAG CCATTGCCTG GTGATAACCA CAACAGATTG 
CGCAATAGCT ACAATGCAGG GCCTCCTTCA CACCTTACAG CTCGAGCCAA TCAGCTACTG 
AAGTTTGGTG CTCCTCATCC TCAATTGGCA ACCCATGGAT CACGGCCCAT TCATCCATTA 
CCTGATTTAT GGACTCCACC TGAAAATCAG CAGCAACCCA GGGGAACTCA CTCTAATTCT 
GGACAATTAA AACGTCCTGG TCCCCCTCTC GGAGAACATT CTGTTATTCA GCATACACCT 
CCTCCCTCTC TACACCGGCC CATAGAAGAC CTCCCCAGTC CGAGCAAGAG GAAGAAGAGC 
TCTGGGTCTG AACAGATTGC GCATTCAGCC ATGCAACGTT TATCTGGACC AACTGTACAT 
TTGAATCAAC AGCCATCCTC CCACTATCCC CCACCTAAAC CTGGCTTTTG GAACCCTTTG 
CACAAAGTGG GAGCACCCTG GAACCCAACT GAACACAAAA GTGGACCCAT TCATTTCCAG 
GATTCAGCTA AGCCAGAAGT TGAAAGTATC CCCTATAAAG CACCTCCTAT AAGTCTATCA 
ACCTTATCTT CTCCCACCAT TAACACCACA AGTGGCTATG GACAAAGCAG GGCCTCTTCA 
TCACAGTCAC AAAAGGCCAC TCAATCACCA CAGTCTCTGG GACCACCTTT ACAGAGCCCT 
CACATCCACC ATTCAGCATA CTCTTATTCC AACAACAGAC CTTCAGACCA GTCCCAAGGA 
GAACCACATG GTACTACTCC ACAAAGAGGA CATCATTTGG ATACCCCACC TCCGCTAATA 
CCTAAATTCT CAGCATCACT GCGGGCAGAT AGGGAGCGTC CTGCACCCTC ACCAGCAAAC 
CACACCACTG TGCCTTACAG TCACCCCCAG TTCCAGCCTC ACCCAGGGCT AGTCCATTCC 
ACCAGCCCAC CAGCCAGCAA CCAGGCCAAG GCCACAGTAC CTCAGCACAA CCTCCACAAA 
CCCTGGAGGA ACCAGGAGTC ACATGACTGT GCAGGAACAG GAGTTTCTCA GATCGCAGCA 
CGCCACTCTC AGGCACATAA TAGTGTTGTG GGTAACCAGG GTCCAAGGAC CCCCCAACAT 
GTGAGCCCAC CTCAGGCTTC TGCTCGAAAA CCTGTCATTA CTCCAAACCT AGAAACTCCT 
CATCCACCTT GCTCCCTGAA ACCATATACT AGTGTTGGAA GCATCCCTAC GATGAGCTCG 
GCACCCCTTA CAACCTCCTC AATCCACAGC ACTCTAAGCA TTGGAAGACC AACGGATTCT 
TCAGTGCTGA TGTCAAACCC AAACCATCTA TCCATGATTG GTCAAAATCC ACCACATCTT 
GGTTATCAGG GTGTTCACCC TGGTGTTGGA ACCCTTCCAC ATCTACATGG AGTGCAATCT 
CAAATTCAGC AGTCCACTCT TACCCACACA GGACAAAGTA GACCTAACTA CACCCCAGTG 
TCCTCTACTC CTTCCACCCT AAACTCAGGG CTTCAGAGAT CAGGGGACAG CGTCATCACC 
AGCAAACCCT CTAACACTCT CCCACAACCG GTCAGTTCAC CTCTAAGTTG CCCTCACCCT 
CCAGCACAAA TAAAGGCCAA CTCAATCTTG CAGCCAATGT CCAGCAGGTC ATACAACTCT 
GTAACCACTG AATCTTCCTC CTCAATTTCT CAGGCAACTA CTACTGTTCC AACTCCAGTT 
TACCCAGGAT CCAGTTTGCA ACCTACTGTC CCATGCTCAA TTGCTGATGC TTTTAATAAA 
CTTGATGCTG AGTTGCAGGG CCACATGCAA GCAGAAGAGA GAAAGAGACA ACAGGAAGAA 
AAGAGAAAAC AGATTGTAGA GAAACAAGAA GTGAACACAA AACCACATTG TGAGTCTGCC 
ATTAGAAGTC TGGAACGTTT GTTGTCAGGT AATACAACTG AGCCTCCCCC TCCTCGTTTG 
TCTCCAGCCA ATGCACCCTC TTCCATCTCG CCTTCTAGCC AGAATTCACC ACCTTATCCC 
TGGCTGAGTA GAGGAGGAGT GCCCCCTCGT CCTTCTGGAA TTTCAGCAAC CATTATAGAA 
CAATCACAAC CACCTCCTCT TACTCCCCAG ACAGAATATG CCCGTGAGAA GCAAAGGCAG 
AGGGAGAAAT TGAAGAGTGG GACACCATCT CAGAATTCTA CTGGGATTTC CACATACCAC 
TCAGAATCTA CTCTCATCAG CACCTCCAAC CACAACATGC AACTGAAACC TGACCCATCC 
AAAGACTTCA TGTTGAAAAC CGCTCAAAAT ATTGGGATAA TTGGTGCTAT TCCTAACCCT 
CCACCCCTGC GAGAGCAACC CAAACTTTAT CAAGCTTTTC ACAAGGATGC TAACCCTTCA 
TGCACATTAA TTAGCATGAC GGAAAACAAC CTTCAAAAAC AAATTCCCAG TGCAGGACTT 
CAAGGTCTGG GCAGCAATGC TAGTAGCTGC AGTGGTGATT CAGATGGTGC CCAATTTGAG 
GAAGAAACTT CTGAGCTTTT GCCTGATGGA TTAGCTAACA TTATGAAGAT GCTGGATGAG 
TCAATCAAGA AAGAGGAGGA GTTGTATTCT GGTCAGAAGG GAGAACAAGC AGTGTCTGAA 
CTTCAATTTT CTACAAATGT GGCTCCTGTG AAGAGTTATT TGTGCGCACC AGACCTTGTG 
CCTGCCCTAA AGCAATCACC AGCTGAGGAT TATCAGCCAG ATGGCCATGC AAGCCCCCCT 
GTGTTAAGTC GACAAGGTTC ACTGGCATCT CCTTGCAGCC GCACATCCTC ACTTGAGGAA 
GAAGAAGAGG TTCTTGCTTC TTCACTCAAG GTCATTCCCA AGTCTGCAAA TTCCAATAGT 
GTTCTGTCCC AGGATGGCAG TCTTGCCACA ACAGGAACCA ACTATCGCCA TAGTGACCTG 
GCCAAGCTTT ATGGCATTTC AGAGGTGGTG AAAAGTGAGT GCGAGGATGA TGAAGATGAA 
GTGGATCGAG AAGATGAAAC TCCATCCTGT TCACCACCTC AGCGCCCCCA CCTTCATCAG 
ACAGGTGTGA ACAGCATGTT TAAAAACCTT GCATCTGTAC TTGAAAGTCA GAAGTACACA 
TACCGGGGTG GTCCCTTTGG ACGTCCTCCT CGTTCTGCTT TTGTGGGAGT GAAGTACTCC 
TCATCTCTTT CTTTGGGGCC TGATATATGC AGGCAGCAAC AAAGCACTTC TCCCACATCA 
GGTTCAGCCA GTCACCCAGG CTTTAGCAGT CCAACGCAAA CCTCTGCCAT TAGCAACTCA 
GGTCAGACCT GTCCCCTCTC ACCTACTAGT TGTGAATCAG AGAGTAAAAG AGGGGATAAG 
ATAAGCCCTT CAGATCTTCT GGGCAATGAT GATGAGAAGG ATATTTCAGA AGAACCCAAA 
GTTAAGGTGG ATTCTGGAAA CATGAAAGAC TTGTTGGAAA GACGACCAAA GCTGACCACC 
ATCTCAGAGT CTTCTCTAGC AGAGCTTGGT CAAAGCTTGG AAGTGAATAA ACACATAATC 
CCTTTTAAAG ATCACTCAAA AGCAGAGGCT CGTGAGATAT GCAAACCTGT TGAAGAAAAG 
GAACGACACA GAGACAGAGA ACGAGAAAGG AAACACAAAC ACAGCAGCAG TAGCAAAAAA 
CATGAGGACA AGAAAGAGAG AAAGAAAAAG CACAGAGAAA GACATGACGA TGCATCCCTT 
TCCTCCTCCT CTTCTTCCAG CTCCAGTCGA AGACACAGGG ACGGAAAGTC ACATAAGGAA 
AAGAAGAGCA GGCAAGTGCT GGGTAATCTG GACCTTCAGA GTAAGGAGCT TAGGGAGAAA 
GAACAAGAGC GAGATGGAGA CAAGAAGAGA AGGAAAGAAG AAAGTGGCCG ACCCCAGAAT 
GAAGGGGAAG CGTGGACTCA GATTAGAGAG AAGGGCAGCA GCTCTGGACA TTCTTCCGAT 
CTCCCATCAG GTTCATCGGC ATTATGTTCG GATGACTTTC AGAAACTGAA AGCACTGACA 
GATGGGCCAC CTAAAGAACT GAAGATTCGT CTCATAAAGG TGGAGAGTGG GGACAGGGAG 
ACATTTATTG CTTCAGAAGT AGAAGAGAAG AGAATTCCTC TGAAAGATAT AACCATAAAG 
AACTCTGCTA GTGAAATTAT CAGAGCCTGC AAGGCAGCTC GTGTGAAGGG GAAATTCAAG 
GAATCCTATC TTCTGGATGC CTTTTCTGTG AAACCTGTTG TGAGGACAGA GCTGCCCATT 
CCTCGTGAGA AACTCAACCC CCCTACACCA AGCATCTATT TGGAAAGTAA AAGAGATGCA 
TTCTCTCCAG TCCTGCTGCA GTTTTGTACA GATCCTAAAA ACCCCATTAC TGTCATCCGG 
GGACTAGCTG GCTCTCTCAG ATTAAATCTT GGTTTGTTTT CCACAAAGTC TTTGGTGGAG 
GCAAATTCAG AGCATGCAGT TGAGGTCCGA ACCCAGGTCC AGCAACCAGC AGATGAGAAC 
TGGGATGCCA GCGGCACCGG ACAGACCTGG CCCTGTGAGA GCAGCAGGTC ACATACAACA 
ATTGCCAAGT ATGCCCAGTA CCAGGCATCT AGCTTCCAAG AGAGCTTACA GGAAGAGAAA 
GGCAGTGACG CTGAGGAGGA TAATGATGAA AAGCCCACCA CTAAAGCGGA AAGTACAACC 
AGTAACTCTT CGGCACCCAA CTCCAGTTCA GAGCAGAAAA CTGTGGGGAA AATAATTAAA 
TTTGGCACAA ACATTGACCT GTCTGATCCC AAAAGATGGA AGCCTCAGCT GCAGGAACTG 
CAGAAGTTGC CAGCATTTAT GCGCGTATCC TCCAGTGGGA ATATGCTAAG CCATGTTGGC 
CACACCATTT TGGGAATGAA TACAGTACAA CTATATATGA AGATTCCTGG ATGCCGCACA 
CCAGGACACC AGGAGAACAA CAACTTCTGT TCTGTTAACA TCAACATTGG ACCTGGAGAC 
TGTGAGTGGT TTGCTGTCCA TGATGACTAC TGGGAGGCCA TCAGTGATTT TTGTCAGAAA 
CATGGAGTGG ACTATTTGAC GGGTTCATGG TGGCCAGTTC TGGATGACTT GTATCGCGCT 
AACATCCCTG TGTACCGTTT CATCCAAAGG CCAGGAGATC TGGTGTGGAT CAACGCAGGC 
ACTGTACACT GGGTTCAGGC CGTGGGCTGG TGCAACAACA TTGCCTGGAA TGTGGGACCC 
GTCAACGCTT ATCAGTACCA GGTGGCTTTA GAAAGATTTG AGTGGAACGA AGTGAAGAAA 
GTGAAATCCA TCGTTCCCAT GATTCATGTG TCCTGGAATG TGGCCCGCAC TGTTAAAGTC 
ACCGACCCAG ACGCTTACAA CATGATCAAA CACTGTCTCC TCCAGTCTAT TAAGCACATT 
CAGATTCTGA GGGATCAGCT GGTGGCAGCA GGAATGAAGA TTTCCTACCA GAGCAGAGTG 
AAGGATGAGC CGGCCTACTA CTGTAACGAG TGTGATGTGG AGGTGTTCAA CCTGCTGTTT 
GTGACTAGTG AGAACGGCAG TCGGAAGATG TACGTGGTGC ACTGTGAGGA CTGTGCACGT 
CAACGCAGCC CAAACCTCTC CAATGTAGTA GTGCTGGAGC AGTACCGCAT GGAGGAGCTC 
ATGAACATGT ACGACTCCTT CAGCCTGGCG GTCAGTCCCA GCTCTCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172296.1
CDS	547..6537
Translation	MHHTAEQFTGCSTRDPFPLDGLNRGPWAPVGSRAWPPPSRCLTGVNQHQLLPHMPSHMTGLNHPSKFLNNGPIHRGDKQDQSQALMPALQRMPPAPPRPWDPTRAGQGYEPLPGDNHNRLRNSYNAGPPSHLTARANQLLKFGAPHPQLATHGSRPIHPLPDLWTPPENQQQPRGTHSNSGQLKRPGPPLGEHSVIQHTPPPSLHRPIEDLPSPSKRKKSSGSEQIAHSAMQRLSGPTVHLNQQPSSHYPPPKPGFWNPLHKVGAPWNPTEHKSGPIHFQDSAKPEVESIPYKAPPISLSTLSSPTINTTSGYGQSRASSSQSQKATQSPQSLGPPLQSPHIHHSAYSYSNNRPSDQSQGEPHGTTPQRGHHLDTPPPLIPKFSASLRADRERPAPSPANHTTVPYSHPQFQPHPGLVHSTSPPASNQAKATVPQHNLHKPWRNQESHDCAGTGVSQIAARHSQAHNSVVGNQGPRTPQHVSPPQASARKPVITPNLETPHPPCSLKPYTSVGSIPTMSSAPLTTSSIHSTLSIGRPTDSSVLMSNPNHLSMIGQNPPHLGYQGVHPGVGTLPHLHGVQSQIQQSTLTHTGQSRPNYTPVSSTPSTLNSGLQRSGDSVITSKPSNTLPQPVSSPLSCPHPPAQIKANSILQPMSSRSYNSVTTESSSSISQATTTVPTPVYPGSSLQPTVPCSIADAFNKLDAELQGHMQAEERKRQQEEKRKQIVEKQEVNTKPHCESAIRSLERLLSGNTTEPPPPRLSPANAPSSISPSSQNSPPYPWLSRGGVPPRPSGISATIIEQSQPPPLTPQTEYAREKQRQREKLKSGTPSQNSTGISTYHSESTLISTSNHNMQLKPDPSKDFMLKTAQNIGIIGAIPNPPPLREQPKLYQAFHKDANPSCTLISMTENNLQKQIPSAGLQGLGSNASSCSGDSDGAQFEEETSELLPDGLANIMKMLDESIKKEEELYSGQKGEQAVSELQFSTNVAPVKSYLCAPDLVPALKQSPAEDYQPDGHASPPVLSRQGSLASPCSRTSSLEEEEEVLASSLKVIPKSANSNSVLSQDGSLATTGTNYRHSDLAKLYGISEVVKSECEDDEDEVDREDETPSCSPPQRPHLHQTGVNSMFKNLASVLESQKYTYRGGPFGRPPRSAFVGVKYSSSLSLGPDICRQQQSTSPTSGSASHPGFSSPTQTSAISNSGQTCPLSPTSCESESKRGDKISPSDLLGNDDEKDISEEPKVKVDSGNMKDLLERRPKLTTISESSLAELGQSLEVNKHIIPFKDHSKAEAREICKPVEEKERHRDRERERKHKHSSSSKKHEDKKERKKKHRERHDDASLSSSSSSSSSRRHRDGKSHKEKKSRQVLGNLDLQSKELREKEQERDGDKKRRKEESGRPQNEGEAWTQIREKGSSSGHSSDLPSGSSALCSDDFQKLKALTDGPPKELKIRLIKVESGDRETFIASEVEEKRIPLKDITIKNSASEIIRACKAARVKGKFKESYLLDAFSVKPVVRTELPIPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPKNPITVIRGLAGSLRLNLGLFSTKSLVEANSEHAVEVRTQVQQPADENWDASGTGQTWPCESSRSHTTIAKYAQYQASSFQESLQEEKGSDAEEDNDEKPTTKAESTTSNSSAPNSSSEQKTVGKIIKFGTNIDLSDPKRWKPQLQELQKLPAFMRVSSSGNMLSHVGHTILGMNTVQLYMKIPGCRTPGHQENNNFCSVNINIGPGDCEWFAVHDDYWEAISDFCQKHGVDYLTGSWWPVLDDLYRANIPVYRFIQRPGDLVWINAGTVHWVQAVGWCNNIAWNVGPVNAYQYQVALERFEWNEVKKVKSIVPMIHVSWNVARTVKVTDPDAYNMIKHCLLQSIKHIQILRDQLVAAGMKISYQSRVKDEPAYYCNECDVEVFNLLFVTSENGSRKMYVVHCEDCARQRSPNLSNVVVLEQYRMEELMNMYDSFSLAVSPSSR

Target ORF information:

RefSeq Version	XM_005172239.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 6B, a (kdm6ba), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172239.4

ORF Insert Sequence:

ATGCATCACA CAGCAGAGCA GTTCACCGGG TGCAGCACAC GGGACCCTTT TCCTCTGGAC 
GGACTCAACC GGGGACCATG GGCTCCCGTG GGCAGCCGTG CCTGGCCACC TCCTTCCAGG 
TGCTTAACTG GTGTCAATCA GCACCAGCTT CTTCCCCATA TGCCCAGTCA CATGACTGGG 
CTAAATCATC CTAGTAAATT CTTGAACAAT GGACCCATAC ATCGAGGGGA TAAGCAAGAC 
CAGTCTCAGG CTTTGATGCC GGCATTGCAA AGAATGCCCC CTGCTCCTCC CAGGCCTTGG 
GATCCAACCA GAGCTGGCCA AGGGTATGAG CCATTGCCTG GTGATAACCA CAACAGATTG 
CGCAATAGCT ACAATGCAGG GCCTCCTTCA CACCTTACAG CTCGAGCCAA TCAGCTACTG 
AAGTTTGGTG CTCCTCATCC TCAATTGGCA ACCCATGGAT CACGGCCCAT TCATCCATTA 
CCTGATTTAT GGACTCCACC TGAAAATCAG CAGCAACCCA GGGGAACTCA CTCTAATTCT 
GGACAATTAA AACGTCCTGG TCCCCCTCTC GGAGAACATT CTGTTATTCA GCATACACCT 
CCTCCCTCTC TACACCGGCC CATAGAAGAC CTCCCCAGTC CGAGCAAGAG GAAGAAGAGC 
TCTGGGTCTG AACAGATTGC GCATTCAGCC ATGCAACGTT TATCTGGACC AACTGTACAT 
TTGAATCAAC AGCCATCCTC CCACTATCCC CCACCTAAAC CTGGCTTTTG GAACCCTTTG 
CACAAAGTGG GAGCACCCTG GAACCCAACT GAACACAAAA GTGGACCCAT TCATTTCCAG 
GATTCAGCTA AGCCAGAAGT TGAAAGTATC CCCTATAAAG CACCTCCTAT AAGTCTATCA 
ACCTTATCTT CTCCCACCAT TAACACCACA AGTGGCTATG GACAAAGCAG GGCCTCTTCA 
TCACAGTCAC AAAAGGCCAC TCAATCACCA CAGTCTCTGG GACCACCTTT ACAGAGCCCT 
CACATCCACC ATTCAGCATA CTCTTATTCC AACAACAGAC CTTCAGACCA GTCCCAAGGA 
GAACCACATG GTACTACTCC ACAAAGAGGA CATCATTTGG ATACCCCACC TCCGCTAATA 
CCTAAATTCT CAGCATCACT GCGGGCAGAT AGGGAGCGTC CTGCACCCTC ACCAGCAAAC 
CACACCACTG TGCCTTACAG TCACCCCCAG TTCCAGCCTC ACCCAGGGCT AGTCCATTCC 
ACCAGCCCAC CAGCCAGCAA CCAGGCCAAG GCCACAGTAC CTCAGCACAA CCTCCACAAA 
CCCTGGAGGA ACCAGGAGTC ACATGACTGT GCAGGAACAG GAGTTTCTCA GATCGCAGCA 
CGCCACTCTC AGGCACATAA TAGTGTTGTG GGTAACCAGG GTCCAAGGAC CCCCCAACAT 
GTGAGCCCAC CTCAGGCTTC TGCTCGAAAA CCTGTCATTA CTCCAAACCT AGAAACTCCT 
CATCCACCTT GCTCCCTGAA ACCATATACT AGTGTTGGAA GCATCCCTAC GATGAGCTCG 
GCACCCCTTA CAACCTCCTC AATCCACAGC ACTCTAAGCA TTGGAAGACC AACGGATTCT 
TCAGTGCTGA TGTCAAACCC AAACCATCTA TCCATGATTG GTCAAAATCC ACCACATCTT 
GGTTATCAGG GTGTTCACCC TGGTGTTGGA ACCCTTCCAC ATCTACATGG AGTGCAATCT 
CAAATTCAGC AGTCCACTCT TACCCACACA GGACAAAGTA GACCTAACTA CACCCCAGTG 
TCCTCTACTC CTTCCACCCT AAACTCAGGG CTTCAGAGAT CAGGGGACAG CGTCATCACC 
AGCAAACCCT CTAACACTCT CCCACAACCG GTCAGTTCAC CTCTAAGTTG CCCTCACCCT 
CCAGCACAAA TAAAGGCCAA CTCAATCTTG CAGCCAATGT CCAGCAGGTC ATACAACTCT 
GTAACCACTG AATCTTCCTC CTCAATTTCT CAGGCAACTA CTACTGTTCC AACTCCAGTT 
TACCCAGGAT CCAGTTTGCA ACCTACTGTC CCATGCTCAA TTGCTGATGC TTTTAATAAA 
CTTGATGCTG AGTTGCAGGG CCACATGCAA GCAGAAGAGA GAAAGAGACA ACAGGAAGAA 
AAGAGAAAAC AGATTGTAGA GAAACAAGAA GTGAACACAA AACCACATTG TGAGTCTGCC 
ATTAGAAGTC TGGAACGTTT GTTGTCAGGT AATACAACTG AGCCTCCCCC TCCTCGTTTG 
TCTCCAGCCA ATGCACCCTC TTCCATCTCG CCTTCTAGCC AGAATTCACC ACCTTATCCC 
TGGCTGAGTA GAGGAGGAGT GCCCCCTCGT CCTTCTGGAA TTTCAGCAAC CATTATAGAA 
CAATCACAAC CACCTCCTCT TACTCCCCAG ACAGAATATG CCCGTGAGAA GCAAAGGCAG 
AGGGAGAAAT TGAAGAGTGG GACACCATCT CAGAATTCTA CTGGGATTTC CACATACCAC 
TCAGAATCTA CTCTCATCAG CACCTCCAAC CACAACATGC AACTGAAACC TGACCCATCC 
AAAGACTTCA TGTTGAAAAC CGCTCAAAAT ATTGGGATAA TTGGTGCTAT TCCTAACCCT 
CCACCCCTGC GAGAGCAACC CAAACTTTAT CAAGCTTTTC ACAAGGATGC TAACCCTTCA 
TGCACATTAA TTAGCATGAC GGAAAACAAC CTTCAAAAAC AAATTCCCAG TGCAGGACTT 
CAAGGTCTGG GCAGCAATGC TAGTAGCTGC AGTGGTGATT CAGATGGTGC CCAATTTGAG 
GAAGAAACTT CTGAGCTTTT GCCTGATGGA TTAGCTAACA TTATGAAGAT GCTGGATGAG 
TCAATCAAGA AAGAGGAGGA GTTGTATTCT GGTCAGAAGG GAGAACAAGC AGTGTCTGAA 
CTTCAATTTT CTACAAATGT GGCTCCTGTG AAGAGTTATT TGTGCGCACC AGACCTTGTG 
CCTGCCCTAA AGCAATCACC AGCTGAGGAT TATCAGCCAG ATGGCCATGC AAGCCCCCCT 
GTGTTAAGTC GACAAGGTTC ACTGGCATCT CCTTGCAGCC GCACATCCTC ACTTGAGGAA 
GAAGAAGAGG TTCTTGCTTC TTCACTCAAG GTCATTCCCA AGTCTGCAAA TTCCAATAGT 
GTTCTGTCCC AGGATGGCAG TCTTGCCACA ACAGGAACCA ACTATCGCCA TAGTGACCTG 
GCCAAGCTTT ATGGCATTTC AGAGGTGGTG AAAAGTGAGT GCGAGGATGA TGAAGATGAA 
GTGGATCGAG AAGATGAAAC TCCATCCTGT TCACCACCTC AGCGCCCCCA CCTTCATCAG 
ACAGGTGTGA ACAGCATGTT TAAAAACCTT GCATCTGTAC TTGAAAGTCA GAAGTACACA 
TACCGGGGTG GTCCCTTTGG ACGTCCTCCT CGTTCTGCTT TTGTGGGAGT GAAGTACTCC 
TCATCTCTTT CTTTGGGGCC TGATATATGC AGGCAGCAAC AAAGCACTTC TCCCACATCA 
GGTTCAGCCA GTCACCCAGG CTTTAGCAGT CCAACGCAAA CCTCTGCCAT TAGCAACTCA 
GGTCAGACCT GTCCCCTCTC ACCTACTAGT TGTGAATCAG AGAGTAAAAG AGGGGATAAG 
ATAAGCCCTT CAGATCTTCT GGGCAATGAT GATGAGAAGG ATATTTCAGA AGAACCCAAA 
GTTAAGGTGG ATTCTGGAAA CATGAAAGAC TTGTTGGAAA GACGACCAAA GCTGACCACC 
ATCTCAGAGT CTTCTCTAGC AGAGCTTGGT CAAAGCTTGG AAGTGAATAA ACACATAATC 
CCTTTTAAAG ATCACTCAAA AGCAGAGGCT CGTGAGATAT GCAAACCTGT TGAAGAAAAG 
GAACGACACA GAGACAGAGA ACGAGAAAGG AAACACAAAC ACAGCAGCAG TAGCAAAAAA 
CATGAGGACA AGAAAGAGAG AAAGAAAAAG CACAGAGAAA GACATGACGA TGCATCCCTT 
TCCTCCTCCT CTTCTTCCAG CTCCAGTCGA AGACACAGGG ACGGAAAGTC ACATAAGGAA 
AAGAAGAGCA GGCAAGTGCT GGGTAATCTG GACCTTCAGA GTAAGGAGCT TAGGGAGAAA 
GAACAAGAGC GAGATGGAGA CAAGAAGAGA AGGAAAGAAG AAAGTGGCCG ACCCCAGAAT 
GAAGGGGAAG CGTGGACTCA GATTAGAGAG AAGGGCAGCA GCTCTGGACA TTCTTCCGAT 
CTCCCATCAG GTTCATCGGC ATTATGTTCG GATGACTTTC AGAAACTGAA AGCACTGACA 
GATGGGCCAC CTAAAGAACT GAAGATTCGT CTCATAAAGG TGGAGAGTGG GGACAGGGAG 
ACATTTATTG CTTCAGAAGT AGAAGAGAAG AGAATTCCTC TGAAAGATAT AACCATAAAG 
AACTCTGCTA GTGAAATTAT CAGAGCCTGC AAGGCAGCTC GTGTGAAGGG GAAATTCAAG 
GAATCCTATC TTCTGGATGC CTTTTCTGTG AAACCTGTTG TGAGGACAGA GCTGCCCATT 
CCTCGTGAGA AACTCAACCC CCCTACACCA AGCATCTATT TGGAAAGTAA AAGAGATGCA 
TTCTCTCCAG TCCTGCTGCA GTTTTGTACA GATCCTAAAA ACCCCATTAC TGTCATCCGG 
GGACTAGCTG GCTCTCTCAG ATTAAATCTT GGTTTGTTTT CCACAAAGTC TTTGGTGGAG 
GCAAATTCAG AGCATGCAGT TGAGGTCCGA ACCCAGGTCC AGCAACCAGC AGATGAGAAC 
TGGGATGCCA GCGGCACCGG ACAGACCTGG CCCTGTGAGA GCAGCAGGTC ACATACAACA 
ATTGCCAAGT ATGCCCAGTA CCAGGCATCT AGCTTCCAAG AGAGCTTACA GGAAGAGAAA 
GGCAGTGACG CTGAGGAGGA TAATGATGAA AAGCCCACCA CTAAAGCGGA AAGTACAACC 
AGTAACTCTT CGGCACCCAA CTCCAGTTCA GAGCAGAAAA CTGTGGGGAA AATAATTAAA 
TTTGGCACAA ACATTGACCT GTCTGATCCC AAAAGATGGA AGCCTCAGCT GCAGGAACTG 
CAGAAGTTGC CAGCATTTAT GCGCGTATCC TCCAGTGGGA ATATGCTAAG CCATGTTGGC 
CACACCATTT TGGGAATGAA TACAGTACAA CTATATATGA AGATTCCTGG ATGCCGCACA 
CCAGGACACC AGGAGAACAA CAACTTCTGT TCTGTTAACA TCAACATTGG ACCTGGAGAC 
TGTGAGTGGT TTGCTGTCCA TGATGACTAC TGGGAGGCCA TCAGTGATTT TTGTCAGAAA 
CATGGAGTGG ACTATTTGAC GGGTTCATGG TGGCCAGTTC TGGATGACTT GTATCGCGCT 
AACATCCCTG TGTACCGTTT CATCCAAAGG CCAGGAGATC TGGTGTGGAT CAACGCAGGC 
ACTGTACACT GGGTTCAGGC CGTGGGCTGG TGCAACAACA TTGCCTGGAA TGTGGGACCC 
GTCAACGCTT ATCAGTACCA GGTGGCTTTA GAAAGATTTG AGTGGAACGA AGTGAAGAAA 
GTGAAATCCA TCGTTCCCAT GATTCATGTG TCCTGGAATG TGGCCCGCAC TGTTAAAGTC 
ACCGACCCAG ACGCTTACAA CATGATCAAA CACTGTCTCC TCCAGTCTAT TAAGCACATT 
CAGATTCTGA GGGATCAGCT GGTGGCAGCA GGAATGAAGA TTTCCTACCA GAGCAGAGTG 
AAGGATGAGC CGGCCTACTA CTGTAACGAG TGTGATGTGG AGGTGTTCAA CCTGCTGTTT 
GTGACTAGTG AGAACGGCAG TCGGAAGATG TACGTGGTGC ACTGTGAGGA CTGTGCACGT 
CAACGCAGCC CAAACCTCTC CAATGTAGTA GTGCTGGAGC AGTACCGCAT GGAGGAGCTC 
ATGAACATGT ACGACTCCTT CAGCCTGGCG GTCAGTCCCA GCTCTCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6BA ( XM_021477779.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021333454.1 Danio rerio lysine (K)-specific demethylase 6B, a (kdm6ba), transcript variant X4, mRNA.

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CloneID	ODa12856
Clone ID Related Accession (Same CDS sequence)	XM_005172239.4 , XM_021477779.1 , XM_021477780.1 , XM_009303125.2 , XM_005172240.3 , XM_017357011.1 , XM_005172240.4 , XM_009303125.3 , XM_005172239.3
Accession Version	XM_021477779.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	lysine-specific demethylase 6B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCACA CAGCAGAGCA GTTCACCGGG TGCAGCACAC GGGACCCTTT TCCTCTGGAC 
GGACTCAACC GGGGACCATG GGCTCCCGTG GGCAGCCGTG CCTGGCCACC TCCTTCCAGG 
TGCTTAACTG GTGTCAATCA GCACCAGCTT CTTCCCCATA TGCCCAGTCA CATGACTGGG 
CTAAATCATC CTAGTAAATT CTTGAACAAT GGACCCATAC ATCGAGGGGA TAAGCAAGAC 
CAGTCTCAGG CTTTGATGCC GGCATTGCAA AGAATGCCCC CTGCTCCTCC CAGGCCTTGG 
GATCCAACCA GAGCTGGCCA AGGGTATGAG CCATTGCCTG GTGATAACCA CAACAGATTG 
CGCAATAGCT ACAATGCAGG GCCTCCTTCA CACCTTACAG CTCGAGCCAA TCAGCTACTG 
AAGTTTGGTG CTCCTCATCC TCAATTGGCA ACCCATGGAT CACGGCCCAT TCATCCATTA 
CCTGATTTAT GGACTCCACC TGAAAATCAG CAGCAACCCA GGGGAACTCA CTCTAATTCT 
GGACAATTAA AACGTCCTGG TCCCCCTCTC GGAGAACATT CTGTTATTCA GCATACACCT 
CCTCCCTCTC TACACCGGCC CATAGAAGAC CTCCCCAGTC CGAGCAAGAG GAAGAAGAGC 
TCTGGGTCTG AACAGATTGC GCATTCAGCC ATGCAACGTT TATCTGGACC AACTGTACAT 
TTGAATCAAC AGCCATCCTC CCACTATCCC CCACCTAAAC CTGGCTTTTG GAACCCTTTG 
CACAAAGTGG GAGCACCCTG GAACCCAACT GAACACAAAA GTGGACCCAT TCATTTCCAG 
GATTCAGCTA AGCCAGAAGT TGAAAGTATC CCCTATAAAG CACCTCCTAT AAGTCTATCA 
ACCTTATCTT CTCCCACCAT TAACACCACA AGTGGCTATG GACAAAGCAG GGCCTCTTCA 
TCACAGTCAC AAAAGGCCAC TCAATCACCA CAGTCTCTGG GACCACCTTT ACAGAGCCCT 
CACATCCACC ATTCAGCATA CTCTTATTCC AACAACAGAC CTTCAGACCA GTCCCAAGGA 
GAACCACATG GTACTACTCC ACAAAGAGGA CATCATTTGG ATACCCCACC TCCGCTAATA 
CCTAAATTCT CAGCATCACT GCGGGCAGAT AGGGAGCGTC CTGCACCCTC ACCAGCAAAC 
CACACCACTG TGCCTTACAG TCACCCCCAG TTCCAGCCTC ACCCAGGGCT AGTCCATTCC 
ACCAGCCCAC CAGCCAGCAA CCAGGCCAAG GCCACAGTAC CTCAGCACAA CCTCCACAAA 
CCCTGGAGGA ACCAGGAGTC ACATGACTGT GCAGGAACAG GAGTTTCTCA GATCGCAGCA 
CGCCACTCTC AGGCACATAA TAGTGTTGTG GGTAACCAGG GTCCAAGGAC CCCCCAACAT 
GTGAGCCCAC CTCAGGCTTC TGCTCGAAAA CCTGTCATTA CTCCAAACCT AGAAACTCCT 
CATCCACCTT GCTCCCTGAA ACCATATACT AGTGTTGGAA GCATCCCTAC GATGAGCTCG 
GCACCCCTTA CAACCTCCTC AATCCACAGC ACTCTAAGCA TTGGAAGACC AACGGATTCT 
TCAGTGCTGA TGTCAAACCC AAACCATCTA TCCATGATTG GTCAAAATCC ACCACATCTT 
GGTTATCAGG GTGTTCACCC TGGTGTTGGA ACCCTTCCAC ATCTACATGG AGTGCAATCT 
CAAATTCAGC AGTCCACTCT TACCCACACA GGACAAAGTA GACCTAACTA CACCCCAGTG 
TCCTCTACTC CTTCCACCCT AAACTCAGGG CTTCAGAGAT CAGGGGACAG CGTCATCACC 
AGCAAACCCT CTAACACTCT CCCACAACCG GTCAGTTCAC CTCTAAGTTG CCCTCACCCT 
CCAGCACAAA TAAAGGCCAA CTCAATCTTG CAGCCAATGT CCAGCAGGTC ATACAACTCT 
GTAACCACTG AATCTTCCTC CTCAATTTCT CAGGCAACTA CTACTGTTCC AACTCCAGTT 
TACCCAGGAT CCAGTTTGCA ACCTACTGTC CCATGCTCAA TTGCTGATGC TTTTAATAAA 
CTTGATGCTG AGTTGCAGGG CCACATGCAA GCAGAAGAGA GAAAGAGACA ACAGGAAGAA 
AAGAGAAAAC AGATTGTAGA GAAACAAGAA GTGAACACAA AACCACATTG TGAGTCTGCC 
ATTAGAAGTC TGGAACGTTT GTTGTCAGGT AATACAACTG AGCCTCCCCC TCCTCGTTTG 
TCTCCAGCCA ATGCACCCTC TTCCATCTCG CCTTCTAGCC AGAATTCACC ACCTTATCCC 
TGGCTGAGTA GAGGAGGAGT GCCCCCTCGT CCTTCTGGAA TTTCAGCAAC CATTATAGAA 
CAATCACAAC CACCTCCTCT TACTCCCCAG ACAGAATATG CCCGTGAGAA GCAAAGGCAG 
AGGGAGAAAT TGAAGAGTGG GACACCATCT CAGAATTCTA CTGGGATTTC CACATACCAC 
TCAGAATCTA CTCTCATCAG CACCTCCAAC CACAACATGC AACTGAAACC TGACCCATCC 
AAAGACTTCA TGTTGAAAAC CGCTCAAAAT ATTGGGATAA TTGGTGCTAT TCCTAACCCT 
CCACCCCTGC GAGAGCAACC CAAACTTTAT CAAGCTTTTC ACAAGGATGC TAACCCTTCA 
TGCACATTAA TTAGCATGAC GGAAAACAAC CTTCAAAAAC AAATTCCCAG TGCAGGACTT 
CAAGGTCTGG GCAGCAATGC TAGTAGCTGC AGTGGTGATT CAGATGGTGC CCAATTTGAG 
GAAGAAACTT CTGAGCTTTT GCCTGATGGA TTAGCTAACA TTATGAAGAT GCTGGATGAG 
TCAATCAAGA AAGAGGAGGA GTTGTATTCT GGTCAGAAGG GAGAACAAGC AGTGTCTGAA 
CTTCAATTTT CTACAAATGT GGCTCCTGTG AAGAGTTATT TGTGCGCACC AGACCTTGTG 
CCTGCCCTAA AGCAATCACC AGCTGAGGAT TATCAGCCAG ATGGCCATGC AAGCCCCCCT 
GTGTTAAGTC GACAAGGTTC ACTGGCATCT CCTTGCAGCC GCACATCCTC ACTTGAGGAA 
GAAGAAGAGG TTCTTGCTTC TTCACTCAAG GTCATTCCCA AGTCTGCAAA TTCCAATAGT 
GTTCTGTCCC AGGATGGCAG TCTTGCCACA ACAGGAACCA ACTATCGCCA TAGTGACCTG 
GCCAAGCTTT ATGGCATTTC AGAGGTGGTG AAAAGTGAGT GCGAGGATGA TGAAGATGAA 
GTGGATCGAG AAGATGAAAC TCCATCCTGT TCACCACCTC AGCGCCCCCA CCTTCATCAG 
ACAGGTGTGA ACAGCATGTT TAAAAACCTT GCATCTGTAC TTGAAAGTCA GAAGTACACA 
TACCGGGGTG GTCCCTTTGG ACGTCCTCCT CGTTCTGCTT TTGTGGGAGT GAAGTACTCC 
TCATCTCTTT CTTTGGGGCC TGATATATGC AGGCAGCAAC AAAGCACTTC TCCCACATCA 
GGTTCAGCCA GTCACCCAGG CTTTAGCAGT CCAACGCAAA CCTCTGCCAT TAGCAACTCA 
GGTCAGACCT GTCCCCTCTC ACCTACTAGT TGTGAATCAG AGAGTAAAAG AGGGGATAAG 
ATAAGCCCTT CAGATCTTCT GGGCAATGAT GATGAGAAGG ATATTTCAGA AGAACCCAAA 
GTTAAGGTGG ATTCTGGAAA CATGAAAGAC TTGTTGGAAA GACGACCAAA GCTGACCACC 
ATCTCAGAGT CTTCTCTAGC AGAGCTTGGT CAAAGCTTGG AAGTGAATAA ACACATAATC 
CCTTTTAAAG ATCACTCAAA AGCAGAGGCT CGTGAGATAT GCAAACCTGT TGAAGAAAAG 
GAACGACACA GAGACAGAGA ACGAGAAAGG AAACACAAAC ACAGCAGCAG TAGCAAAAAA 
CATGAGGACA AGAAAGAGAG AAAGAAAAAG CACAGAGAAA GACATGACGA TGCATCCCTT 
TCCTCCTCCT CTTCTTCCAG CTCCAGTCGA AGACACAGGG ACGGAAAGTC ACATAAGGAA 
AAGAAGAGCA GGCAAGTGCT GGGTAATCTG GACCTTCAGA GTAAGGAGCT TAGGGAGAAA 
GAACAAGAGC GAGATGGAGA CAAGAAGAGA AGGAAAGAAG AAAGTGGCCG ACCCCAGAAT 
GAAGGGGAAG CGTGGACTCA GATTAGAGAG AAGGGCAGCA GCTCTGGACA TTCTTCCGAT 
CTCCCATCAG GTTCATCGGC ATTATGTTCG GATGACTTTC AGAAACTGAA AGCACTGACA 
GATGGGCCAC CTAAAGAACT GAAGATTCGT CTCATAAAGG TGGAGAGTGG GGACAGGGAG 
ACATTTATTG CTTCAGAAGT AGAAGAGAAG AGAATTCCTC TGAAAGATAT AACCATAAAG 
AACTCTGCTA GTGAAATTAT CAGAGCCTGC AAGGCAGCTC GTGTGAAGGG GAAATTCAAG 
GAATCCTATC TTCTGGATGC CTTTTCTGTG AAACCTGTTG TGAGGACAGA GCTGCCCATT 
CCTCGTGAGA AACTCAACCC CCCTACACCA AGCATCTATT TGGAAAGTAA AAGAGATGCA 
TTCTCTCCAG TCCTGCTGCA GTTTTGTACA GATCCTAAAA ACCCCATTAC TGTCATCCGG 
GGACTAGCTG GCTCTCTCAG ATTAAATCTT GGTTTGTTTT CCACAAAGTC TTTGGTGGAG 
GCAAATTCAG AGCATGCAGT TGAGGTCCGA ACCCAGGTCC AGCAACCAGC AGATGAGAAC 
TGGGATGCCA GCGGCACCGG ACAGACCTGG CCCTGTGAGA GCAGCAGGTC ACATACAACA 
ATTGCCAAGT ATGCCCAGTA CCAGGCATCT AGCTTCCAAG AGAGCTTACA GGAAGAGAAA 
GGCAGTGACG CTGAGGAGGA TAATGATGAA AAGCCCACCA CTAAAGCGGA AAGTACAACC 
AGTAACTCTT CGGCACCCAA CTCCAGTTCA GAGCAGAAAA CTGTGGGGAA AATAATTAAA 
TTTGGCACAA ACATTGACCT GTCTGATCCC AAAAGATGGA AGCCTCAGCT GCAGGAACTG 
CAGAAGTTGC CAGCATTTAT GCGCGTATCC TCCAGTGGGA ATATGCTAAG CCATGTTGGC 
CACACCATTT TGGGAATGAA TACAGTACAA CTATATATGA AGATTCCTGG ATGCCGCACA 
CCAGGACACC AGGAGAACAA CAACTTCTGT TCTGTTAACA TCAACATTGG ACCTGGAGAC 
TGTGAGTGGT TTGCTGTCCA TGATGACTAC TGGGAGGCCA TCAGTGATTT TTGTCAGAAA 
CATGGAGTGG ACTATTTGAC GGGTTCATGG TGGCCAGTTC TGGATGACTT GTATCGCGCT 
AACATCCCTG TGTACCGTTT CATCCAAAGG CCAGGAGATC TGGTGTGGAT CAACGCAGGC 
ACTGTACACT GGGTTCAGGC CGTGGGCTGG TGCAACAACA TTGCCTGGAA TGTGGGACCC 
GTCAACGCTT ATCAGTACCA GGTGGCTTTA GAAAGATTTG AGTGGAACGA AGTGAAGAAA 
GTGAAATCCA TCGTTCCCAT GATTCATGTG TCCTGGAATG TGGCCCGCAC TGTTAAAGTC 
ACCGACCCAG ACGCTTACAA CATGATCAAA CACTGTCTCC TCCAGTCTAT TAAGCACATT 
CAGATTCTGA GGGATCAGCT GGTGGCAGCA GGAATGAAGA TTTCCTACCA GAGCAGAGTG 
AAGGATGAGC CGGCCTACTA CTGTAACGAG TGTGATGTGG AGGTGTTCAA CCTGCTGTTT 
GTGACTAGTG AGAACGGCAG TCGGAAGATG TACGTGGTGC ACTGTGAGGA CTGTGCACGT 
CAACGCAGCC CAAACCTCTC CAATGTAGTA GTGCTGGAGC AGTACCGCAT GGAGGAGCTC 
ATGAACATGT ACGACTCCTT CAGCCTGGCG GTCAGTCCCA GCTCTCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333454.1
CDS	634..6624
Translation	MHHTAEQFTGCSTRDPFPLDGLNRGPWAPVGSRAWPPPSRCLTGVNQHQLLPHMPSHMTGLNHPSKFLNNGPIHRGDKQDQSQALMPALQRMPPAPPRPWDPTRAGQGYEPLPGDNHNRLRNSYNAGPPSHLTARANQLLKFGAPHPQLATHGSRPIHPLPDLWTPPENQQQPRGTHSNSGQLKRPGPPLGEHSVIQHTPPPSLHRPIEDLPSPSKRKKSSGSEQIAHSAMQRLSGPTVHLNQQPSSHYPPPKPGFWNPLHKVGAPWNPTEHKSGPIHFQDSAKPEVESIPYKAPPISLSTLSSPTINTTSGYGQSRASSSQSQKATQSPQSLGPPLQSPHIHHSAYSYSNNRPSDQSQGEPHGTTPQRGHHLDTPPPLIPKFSASLRADRERPAPSPANHTTVPYSHPQFQPHPGLVHSTSPPASNQAKATVPQHNLHKPWRNQESHDCAGTGVSQIAARHSQAHNSVVGNQGPRTPQHVSPPQASARKPVITPNLETPHPPCSLKPYTSVGSIPTMSSAPLTTSSIHSTLSIGRPTDSSVLMSNPNHLSMIGQNPPHLGYQGVHPGVGTLPHLHGVQSQIQQSTLTHTGQSRPNYTPVSSTPSTLNSGLQRSGDSVITSKPSNTLPQPVSSPLSCPHPPAQIKANSILQPMSSRSYNSVTTESSSSISQATTTVPTPVYPGSSLQPTVPCSIADAFNKLDAELQGHMQAEERKRQQEEKRKQIVEKQEVNTKPHCESAIRSLERLLSGNTTEPPPPRLSPANAPSSISPSSQNSPPYPWLSRGGVPPRPSGISATIIEQSQPPPLTPQTEYAREKQRQREKLKSGTPSQNSTGISTYHSESTLISTSNHNMQLKPDPSKDFMLKTAQNIGIIGAIPNPPPLREQPKLYQAFHKDANPSCTLISMTENNLQKQIPSAGLQGLGSNASSCSGDSDGAQFEEETSELLPDGLANIMKMLDESIKKEEELYSGQKGEQAVSELQFSTNVAPVKSYLCAPDLVPALKQSPAEDYQPDGHASPPVLSRQGSLASPCSRTSSLEEEEEVLASSLKVIPKSANSNSVLSQDGSLATTGTNYRHSDLAKLYGISEVVKSECEDDEDEVDREDETPSCSPPQRPHLHQTGVNSMFKNLASVLESQKYTYRGGPFGRPPRSAFVGVKYSSSLSLGPDICRQQQSTSPTSGSASHPGFSSPTQTSAISNSGQTCPLSPTSCESESKRGDKISPSDLLGNDDEKDISEEPKVKVDSGNMKDLLERRPKLTTISESSLAELGQSLEVNKHIIPFKDHSKAEAREICKPVEEKERHRDRERERKHKHSSSSKKHEDKKERKKKHRERHDDASLSSSSSSSSSRRHRDGKSHKEKKSRQVLGNLDLQSKELREKEQERDGDKKRRKEESGRPQNEGEAWTQIREKGSSSGHSSDLPSGSSALCSDDFQKLKALTDGPPKELKIRLIKVESGDRETFIASEVEEKRIPLKDITIKNSASEIIRACKAARVKGKFKESYLLDAFSVKPVVRTELPIPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPKNPITVIRGLAGSLRLNLGLFSTKSLVEANSEHAVEVRTQVQQPADENWDASGTGQTWPCESSRSHTTIAKYAQYQASSFQESLQEEKGSDAEEDNDEKPTTKAESTTSNSSAPNSSSEQKTVGKIIKFGTNIDLSDPKRWKPQLQELQKLPAFMRVSSSGNMLSHVGHTILGMNTVQLYMKIPGCRTPGHQENNNFCSVNINIGPGDCEWFAVHDDYWEAISDFCQKHGVDYLTGSWWPVLDDLYRANIPVYRFIQRPGDLVWINAGTVHWVQAVGWCNNIAWNVGPVNAYQYQVALERFEWNEVKKVKSIVPMIHVSWNVARTVKVTDPDAYNMIKHCLLQSIKHIQILRDQLVAAGMKISYQSRVKDEPAYYCNECDVEVFNLLFVTSENGSRKMYVVHCEDCARQRSPNLSNVVVLEQYRMEELMNMYDSFSLAVSPSSR

Target ORF information:

RefSeq Version	XM_021477779.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 6B, a (kdm6ba), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477779.1

ORF Insert Sequence:

ATGCATCACA CAGCAGAGCA GTTCACCGGG TGCAGCACAC GGGACCCTTT TCCTCTGGAC 
GGACTCAACC GGGGACCATG GGCTCCCGTG GGCAGCCGTG CCTGGCCACC TCCTTCCAGG 
TGCTTAACTG GTGTCAATCA GCACCAGCTT CTTCCCCATA TGCCCAGTCA CATGACTGGG 
CTAAATCATC CTAGTAAATT CTTGAACAAT GGACCCATAC ATCGAGGGGA TAAGCAAGAC 
CAGTCTCAGG CTTTGATGCC GGCATTGCAA AGAATGCCCC CTGCTCCTCC CAGGCCTTGG 
GATCCAACCA GAGCTGGCCA AGGGTATGAG CCATTGCCTG GTGATAACCA CAACAGATTG 
CGCAATAGCT ACAATGCAGG GCCTCCTTCA CACCTTACAG CTCGAGCCAA TCAGCTACTG 
AAGTTTGGTG CTCCTCATCC TCAATTGGCA ACCCATGGAT CACGGCCCAT TCATCCATTA 
CCTGATTTAT GGACTCCACC TGAAAATCAG CAGCAACCCA GGGGAACTCA CTCTAATTCT 
GGACAATTAA AACGTCCTGG TCCCCCTCTC GGAGAACATT CTGTTATTCA GCATACACCT 
CCTCCCTCTC TACACCGGCC CATAGAAGAC CTCCCCAGTC CGAGCAAGAG GAAGAAGAGC 
TCTGGGTCTG AACAGATTGC GCATTCAGCC ATGCAACGTT TATCTGGACC AACTGTACAT 
TTGAATCAAC AGCCATCCTC CCACTATCCC CCACCTAAAC CTGGCTTTTG GAACCCTTTG 
CACAAAGTGG GAGCACCCTG GAACCCAACT GAACACAAAA GTGGACCCAT TCATTTCCAG 
GATTCAGCTA AGCCAGAAGT TGAAAGTATC CCCTATAAAG CACCTCCTAT AAGTCTATCA 
ACCTTATCTT CTCCCACCAT TAACACCACA AGTGGCTATG GACAAAGCAG GGCCTCTTCA 
TCACAGTCAC AAAAGGCCAC TCAATCACCA CAGTCTCTGG GACCACCTTT ACAGAGCCCT 
CACATCCACC ATTCAGCATA CTCTTATTCC AACAACAGAC CTTCAGACCA GTCCCAAGGA 
GAACCACATG GTACTACTCC ACAAAGAGGA CATCATTTGG ATACCCCACC TCCGCTAATA 
CCTAAATTCT CAGCATCACT GCGGGCAGAT AGGGAGCGTC CTGCACCCTC ACCAGCAAAC 
CACACCACTG TGCCTTACAG TCACCCCCAG TTCCAGCCTC ACCCAGGGCT AGTCCATTCC 
ACCAGCCCAC CAGCCAGCAA CCAGGCCAAG GCCACAGTAC CTCAGCACAA CCTCCACAAA 
CCCTGGAGGA ACCAGGAGTC ACATGACTGT GCAGGAACAG GAGTTTCTCA GATCGCAGCA 
CGCCACTCTC AGGCACATAA TAGTGTTGTG GGTAACCAGG GTCCAAGGAC CCCCCAACAT 
GTGAGCCCAC CTCAGGCTTC TGCTCGAAAA CCTGTCATTA CTCCAAACCT AGAAACTCCT 
CATCCACCTT GCTCCCTGAA ACCATATACT AGTGTTGGAA GCATCCCTAC GATGAGCTCG 
GCACCCCTTA CAACCTCCTC AATCCACAGC ACTCTAAGCA TTGGAAGACC AACGGATTCT 
TCAGTGCTGA TGTCAAACCC AAACCATCTA TCCATGATTG GTCAAAATCC ACCACATCTT 
GGTTATCAGG GTGTTCACCC TGGTGTTGGA ACCCTTCCAC ATCTACATGG AGTGCAATCT 
CAAATTCAGC AGTCCACTCT TACCCACACA GGACAAAGTA GACCTAACTA CACCCCAGTG 
TCCTCTACTC CTTCCACCCT AAACTCAGGG CTTCAGAGAT CAGGGGACAG CGTCATCACC 
AGCAAACCCT CTAACACTCT CCCACAACCG GTCAGTTCAC CTCTAAGTTG CCCTCACCCT 
CCAGCACAAA TAAAGGCCAA CTCAATCTTG CAGCCAATGT CCAGCAGGTC ATACAACTCT 
GTAACCACTG AATCTTCCTC CTCAATTTCT CAGGCAACTA CTACTGTTCC AACTCCAGTT 
TACCCAGGAT CCAGTTTGCA ACCTACTGTC CCATGCTCAA TTGCTGATGC TTTTAATAAA 
CTTGATGCTG AGTTGCAGGG CCACATGCAA GCAGAAGAGA GAAAGAGACA ACAGGAAGAA 
AAGAGAAAAC AGATTGTAGA GAAACAAGAA GTGAACACAA AACCACATTG TGAGTCTGCC 
ATTAGAAGTC TGGAACGTTT GTTGTCAGGT AATACAACTG AGCCTCCCCC TCCTCGTTTG 
TCTCCAGCCA ATGCACCCTC TTCCATCTCG CCTTCTAGCC AGAATTCACC ACCTTATCCC 
TGGCTGAGTA GAGGAGGAGT GCCCCCTCGT CCTTCTGGAA TTTCAGCAAC CATTATAGAA 
CAATCACAAC CACCTCCTCT TACTCCCCAG ACAGAATATG CCCGTGAGAA GCAAAGGCAG 
AGGGAGAAAT TGAAGAGTGG GACACCATCT CAGAATTCTA CTGGGATTTC CACATACCAC 
TCAGAATCTA CTCTCATCAG CACCTCCAAC CACAACATGC AACTGAAACC TGACCCATCC 
AAAGACTTCA TGTTGAAAAC CGCTCAAAAT ATTGGGATAA TTGGTGCTAT TCCTAACCCT 
CCACCCCTGC GAGAGCAACC CAAACTTTAT CAAGCTTTTC ACAAGGATGC TAACCCTTCA 
TGCACATTAA TTAGCATGAC GGAAAACAAC CTTCAAAAAC AAATTCCCAG TGCAGGACTT 
CAAGGTCTGG GCAGCAATGC TAGTAGCTGC AGTGGTGATT CAGATGGTGC CCAATTTGAG 
GAAGAAACTT CTGAGCTTTT GCCTGATGGA TTAGCTAACA TTATGAAGAT GCTGGATGAG 
TCAATCAAGA AAGAGGAGGA GTTGTATTCT GGTCAGAAGG GAGAACAAGC AGTGTCTGAA 
CTTCAATTTT CTACAAATGT GGCTCCTGTG AAGAGTTATT TGTGCGCACC AGACCTTGTG 
CCTGCCCTAA AGCAATCACC AGCTGAGGAT TATCAGCCAG ATGGCCATGC AAGCCCCCCT 
GTGTTAAGTC GACAAGGTTC ACTGGCATCT CCTTGCAGCC GCACATCCTC ACTTGAGGAA 
GAAGAAGAGG TTCTTGCTTC TTCACTCAAG GTCATTCCCA AGTCTGCAAA TTCCAATAGT 
GTTCTGTCCC AGGATGGCAG TCTTGCCACA ACAGGAACCA ACTATCGCCA TAGTGACCTG 
GCCAAGCTTT ATGGCATTTC AGAGGTGGTG AAAAGTGAGT GCGAGGATGA TGAAGATGAA 
GTGGATCGAG AAGATGAAAC TCCATCCTGT TCACCACCTC AGCGCCCCCA CCTTCATCAG 
ACAGGTGTGA ACAGCATGTT TAAAAACCTT GCATCTGTAC TTGAAAGTCA GAAGTACACA 
TACCGGGGTG GTCCCTTTGG ACGTCCTCCT CGTTCTGCTT TTGTGGGAGT GAAGTACTCC 
TCATCTCTTT CTTTGGGGCC TGATATATGC AGGCAGCAAC AAAGCACTTC TCCCACATCA 
GGTTCAGCCA GTCACCCAGG CTTTAGCAGT CCAACGCAAA CCTCTGCCAT TAGCAACTCA 
GGTCAGACCT GTCCCCTCTC ACCTACTAGT TGTGAATCAG AGAGTAAAAG AGGGGATAAG 
ATAAGCCCTT CAGATCTTCT GGGCAATGAT GATGAGAAGG ATATTTCAGA AGAACCCAAA 
GTTAAGGTGG ATTCTGGAAA CATGAAAGAC TTGTTGGAAA GACGACCAAA GCTGACCACC 
ATCTCAGAGT CTTCTCTAGC AGAGCTTGGT CAAAGCTTGG AAGTGAATAA ACACATAATC 
CCTTTTAAAG ATCACTCAAA AGCAGAGGCT CGTGAGATAT GCAAACCTGT TGAAGAAAAG 
GAACGACACA GAGACAGAGA ACGAGAAAGG AAACACAAAC ACAGCAGCAG TAGCAAAAAA 
CATGAGGACA AGAAAGAGAG AAAGAAAAAG CACAGAGAAA GACATGACGA TGCATCCCTT 
TCCTCCTCCT CTTCTTCCAG CTCCAGTCGA AGACACAGGG ACGGAAAGTC ACATAAGGAA 
AAGAAGAGCA GGCAAGTGCT GGGTAATCTG GACCTTCAGA GTAAGGAGCT TAGGGAGAAA 
GAACAAGAGC GAGATGGAGA CAAGAAGAGA AGGAAAGAAG AAAGTGGCCG ACCCCAGAAT 
GAAGGGGAAG CGTGGACTCA GATTAGAGAG AAGGGCAGCA GCTCTGGACA TTCTTCCGAT 
CTCCCATCAG GTTCATCGGC ATTATGTTCG GATGACTTTC AGAAACTGAA AGCACTGACA 
GATGGGCCAC CTAAAGAACT GAAGATTCGT CTCATAAAGG TGGAGAGTGG GGACAGGGAG 
ACATTTATTG CTTCAGAAGT AGAAGAGAAG AGAATTCCTC TGAAAGATAT AACCATAAAG 
AACTCTGCTA GTGAAATTAT CAGAGCCTGC AAGGCAGCTC GTGTGAAGGG GAAATTCAAG 
GAATCCTATC TTCTGGATGC CTTTTCTGTG AAACCTGTTG TGAGGACAGA GCTGCCCATT 
CCTCGTGAGA AACTCAACCC CCCTACACCA AGCATCTATT TGGAAAGTAA AAGAGATGCA 
TTCTCTCCAG TCCTGCTGCA GTTTTGTACA GATCCTAAAA ACCCCATTAC TGTCATCCGG 
GGACTAGCTG GCTCTCTCAG ATTAAATCTT GGTTTGTTTT CCACAAAGTC TTTGGTGGAG 
GCAAATTCAG AGCATGCAGT TGAGGTCCGA ACCCAGGTCC AGCAACCAGC AGATGAGAAC 
TGGGATGCCA GCGGCACCGG ACAGACCTGG CCCTGTGAGA GCAGCAGGTC ACATACAACA 
ATTGCCAAGT ATGCCCAGTA CCAGGCATCT AGCTTCCAAG AGAGCTTACA GGAAGAGAAA 
GGCAGTGACG CTGAGGAGGA TAATGATGAA AAGCCCACCA CTAAAGCGGA AAGTACAACC 
AGTAACTCTT CGGCACCCAA CTCCAGTTCA GAGCAGAAAA CTGTGGGGAA AATAATTAAA 
TTTGGCACAA ACATTGACCT GTCTGATCCC AAAAGATGGA AGCCTCAGCT GCAGGAACTG 
CAGAAGTTGC CAGCATTTAT GCGCGTATCC TCCAGTGGGA ATATGCTAAG CCATGTTGGC 
CACACCATTT TGGGAATGAA TACAGTACAA CTATATATGA AGATTCCTGG ATGCCGCACA 
CCAGGACACC AGGAGAACAA CAACTTCTGT TCTGTTAACA TCAACATTGG ACCTGGAGAC 
TGTGAGTGGT TTGCTGTCCA TGATGACTAC TGGGAGGCCA TCAGTGATTT TTGTCAGAAA 
CATGGAGTGG ACTATTTGAC GGGTTCATGG TGGCCAGTTC TGGATGACTT GTATCGCGCT 
AACATCCCTG TGTACCGTTT CATCCAAAGG CCAGGAGATC TGGTGTGGAT CAACGCAGGC 
ACTGTACACT GGGTTCAGGC CGTGGGCTGG TGCAACAACA TTGCCTGGAA TGTGGGACCC 
GTCAACGCTT ATCAGTACCA GGTGGCTTTA GAAAGATTTG AGTGGAACGA AGTGAAGAAA 
GTGAAATCCA TCGTTCCCAT GATTCATGTG TCCTGGAATG TGGCCCGCAC TGTTAAAGTC 
ACCGACCCAG ACGCTTACAA CATGATCAAA CACTGTCTCC TCCAGTCTAT TAAGCACATT 
CAGATTCTGA GGGATCAGCT GGTGGCAGCA GGAATGAAGA TTTCCTACCA GAGCAGAGTG 
AAGGATGAGC CGGCCTACTA CTGTAACGAG TGTGATGTGG AGGTGTTCAA CCTGCTGTTT 
GTGACTAGTG AGAACGGCAG TCGGAAGATG TACGTGGTGC ACTGTGAGGA CTGTGCACGT 
CAACGCAGCC CAAACCTCTC CAATGTAGTA GTGCTGGAGC AGTACCGCAT GGAGGAGCTC 
ATGAACATGT ACGACTCCTT CAGCCTGGCG GTCAGTCCCA GCTCTCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6BA ( XM_021477780.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021333455.1 Danio rerio lysine (K)-specific demethylase 6B, a (kdm6ba), transcript variant X5, mRNA.

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CloneID	ODa12856
Clone ID Related Accession (Same CDS sequence)	XM_005172239.4 , XM_021477779.1 , XM_021477780.1 , XM_009303125.2 , XM_005172240.3 , XM_017357011.1 , XM_005172240.4 , XM_009303125.3 , XM_005172239.3
Accession Version	XM_021477780.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	lysine-specific demethylase 6B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCACA CAGCAGAGCA GTTCACCGGG TGCAGCACAC GGGACCCTTT TCCTCTGGAC 
GGACTCAACC GGGGACCATG GGCTCCCGTG GGCAGCCGTG CCTGGCCACC TCCTTCCAGG 
TGCTTAACTG GTGTCAATCA GCACCAGCTT CTTCCCCATA TGCCCAGTCA CATGACTGGG 
CTAAATCATC CTAGTAAATT CTTGAACAAT GGACCCATAC ATCGAGGGGA TAAGCAAGAC 
CAGTCTCAGG CTTTGATGCC GGCATTGCAA AGAATGCCCC CTGCTCCTCC CAGGCCTTGG 
GATCCAACCA GAGCTGGCCA AGGGTATGAG CCATTGCCTG GTGATAACCA CAACAGATTG 
CGCAATAGCT ACAATGCAGG GCCTCCTTCA CACCTTACAG CTCGAGCCAA TCAGCTACTG 
AAGTTTGGTG CTCCTCATCC TCAATTGGCA ACCCATGGAT CACGGCCCAT TCATCCATTA 
CCTGATTTAT GGACTCCACC TGAAAATCAG CAGCAACCCA GGGGAACTCA CTCTAATTCT 
GGACAATTAA AACGTCCTGG TCCCCCTCTC GGAGAACATT CTGTTATTCA GCATACACCT 
CCTCCCTCTC TACACCGGCC CATAGAAGAC CTCCCCAGTC CGAGCAAGAG GAAGAAGAGC 
TCTGGGTCTG AACAGATTGC GCATTCAGCC ATGCAACGTT TATCTGGACC AACTGTACAT 
TTGAATCAAC AGCCATCCTC CCACTATCCC CCACCTAAAC CTGGCTTTTG GAACCCTTTG 
CACAAAGTGG GAGCACCCTG GAACCCAACT GAACACAAAA GTGGACCCAT TCATTTCCAG 
GATTCAGCTA AGCCAGAAGT TGAAAGTATC CCCTATAAAG CACCTCCTAT AAGTCTATCA 
ACCTTATCTT CTCCCACCAT TAACACCACA AGTGGCTATG GACAAAGCAG GGCCTCTTCA 
TCACAGTCAC AAAAGGCCAC TCAATCACCA CAGTCTCTGG GACCACCTTT ACAGAGCCCT 
CACATCCACC ATTCAGCATA CTCTTATTCC AACAACAGAC CTTCAGACCA GTCCCAAGGA 
GAACCACATG GTACTACTCC ACAAAGAGGA CATCATTTGG ATACCCCACC TCCGCTAATA 
CCTAAATTCT CAGCATCACT GCGGGCAGAT AGGGAGCGTC CTGCACCCTC ACCAGCAAAC 
CACACCACTG TGCCTTACAG TCACCCCCAG TTCCAGCCTC ACCCAGGGCT AGTCCATTCC 
ACCAGCCCAC CAGCCAGCAA CCAGGCCAAG GCCACAGTAC CTCAGCACAA CCTCCACAAA 
CCCTGGAGGA ACCAGGAGTC ACATGACTGT GCAGGAACAG GAGTTTCTCA GATCGCAGCA 
CGCCACTCTC AGGCACATAA TAGTGTTGTG GGTAACCAGG GTCCAAGGAC CCCCCAACAT 
GTGAGCCCAC CTCAGGCTTC TGCTCGAAAA CCTGTCATTA CTCCAAACCT AGAAACTCCT 
CATCCACCTT GCTCCCTGAA ACCATATACT AGTGTTGGAA GCATCCCTAC GATGAGCTCG 
GCACCCCTTA CAACCTCCTC AATCCACAGC ACTCTAAGCA TTGGAAGACC AACGGATTCT 
TCAGTGCTGA TGTCAAACCC AAACCATCTA TCCATGATTG GTCAAAATCC ACCACATCTT 
GGTTATCAGG GTGTTCACCC TGGTGTTGGA ACCCTTCCAC ATCTACATGG AGTGCAATCT 
CAAATTCAGC AGTCCACTCT TACCCACACA GGACAAAGTA GACCTAACTA CACCCCAGTG 
TCCTCTACTC CTTCCACCCT AAACTCAGGG CTTCAGAGAT CAGGGGACAG CGTCATCACC 
AGCAAACCCT CTAACACTCT CCCACAACCG GTCAGTTCAC CTCTAAGTTG CCCTCACCCT 
CCAGCACAAA TAAAGGCCAA CTCAATCTTG CAGCCAATGT CCAGCAGGTC ATACAACTCT 
GTAACCACTG AATCTTCCTC CTCAATTTCT CAGGCAACTA CTACTGTTCC AACTCCAGTT 
TACCCAGGAT CCAGTTTGCA ACCTACTGTC CCATGCTCAA TTGCTGATGC TTTTAATAAA 
CTTGATGCTG AGTTGCAGGG CCACATGCAA GCAGAAGAGA GAAAGAGACA ACAGGAAGAA 
AAGAGAAAAC AGATTGTAGA GAAACAAGAA GTGAACACAA AACCACATTG TGAGTCTGCC 
ATTAGAAGTC TGGAACGTTT GTTGTCAGGT AATACAACTG AGCCTCCCCC TCCTCGTTTG 
TCTCCAGCCA ATGCACCCTC TTCCATCTCG CCTTCTAGCC AGAATTCACC ACCTTATCCC 
TGGCTGAGTA GAGGAGGAGT GCCCCCTCGT CCTTCTGGAA TTTCAGCAAC CATTATAGAA 
CAATCACAAC CACCTCCTCT TACTCCCCAG ACAGAATATG CCCGTGAGAA GCAAAGGCAG 
AGGGAGAAAT TGAAGAGTGG GACACCATCT CAGAATTCTA CTGGGATTTC CACATACCAC 
TCAGAATCTA CTCTCATCAG CACCTCCAAC CACAACATGC AACTGAAACC TGACCCATCC 
AAAGACTTCA TGTTGAAAAC CGCTCAAAAT ATTGGGATAA TTGGTGCTAT TCCTAACCCT 
CCACCCCTGC GAGAGCAACC CAAACTTTAT CAAGCTTTTC ACAAGGATGC TAACCCTTCA 
TGCACATTAA TTAGCATGAC GGAAAACAAC CTTCAAAAAC AAATTCCCAG TGCAGGACTT 
CAAGGTCTGG GCAGCAATGC TAGTAGCTGC AGTGGTGATT CAGATGGTGC CCAATTTGAG 
GAAGAAACTT CTGAGCTTTT GCCTGATGGA TTAGCTAACA TTATGAAGAT GCTGGATGAG 
TCAATCAAGA AAGAGGAGGA GTTGTATTCT GGTCAGAAGG GAGAACAAGC AGTGTCTGAA 
CTTCAATTTT CTACAAATGT GGCTCCTGTG AAGAGTTATT TGTGCGCACC AGACCTTGTG 
CCTGCCCTAA AGCAATCACC AGCTGAGGAT TATCAGCCAG ATGGCCATGC AAGCCCCCCT 
GTGTTAAGTC GACAAGGTTC ACTGGCATCT CCTTGCAGCC GCACATCCTC ACTTGAGGAA 
GAAGAAGAGG TTCTTGCTTC TTCACTCAAG GTCATTCCCA AGTCTGCAAA TTCCAATAGT 
GTTCTGTCCC AGGATGGCAG TCTTGCCACA ACAGGAACCA ACTATCGCCA TAGTGACCTG 
GCCAAGCTTT ATGGCATTTC AGAGGTGGTG AAAAGTGAGT GCGAGGATGA TGAAGATGAA 
GTGGATCGAG AAGATGAAAC TCCATCCTGT TCACCACCTC AGCGCCCCCA CCTTCATCAG 
ACAGGTGTGA ACAGCATGTT TAAAAACCTT GCATCTGTAC TTGAAAGTCA GAAGTACACA 
TACCGGGGTG GTCCCTTTGG ACGTCCTCCT CGTTCTGCTT TTGTGGGAGT GAAGTACTCC 
TCATCTCTTT CTTTGGGGCC TGATATATGC AGGCAGCAAC AAAGCACTTC TCCCACATCA 
GGTTCAGCCA GTCACCCAGG CTTTAGCAGT CCAACGCAAA CCTCTGCCAT TAGCAACTCA 
GGTCAGACCT GTCCCCTCTC ACCTACTAGT TGTGAATCAG AGAGTAAAAG AGGGGATAAG 
ATAAGCCCTT CAGATCTTCT GGGCAATGAT GATGAGAAGG ATATTTCAGA AGAACCCAAA 
GTTAAGGTGG ATTCTGGAAA CATGAAAGAC TTGTTGGAAA GACGACCAAA GCTGACCACC 
ATCTCAGAGT CTTCTCTAGC AGAGCTTGGT CAAAGCTTGG AAGTGAATAA ACACATAATC 
CCTTTTAAAG ATCACTCAAA AGCAGAGGCT CGTGAGATAT GCAAACCTGT TGAAGAAAAG 
GAACGACACA GAGACAGAGA ACGAGAAAGG AAACACAAAC ACAGCAGCAG TAGCAAAAAA 
CATGAGGACA AGAAAGAGAG AAAGAAAAAG CACAGAGAAA GACATGACGA TGCATCCCTT 
TCCTCCTCCT CTTCTTCCAG CTCCAGTCGA AGACACAGGG ACGGAAAGTC ACATAAGGAA 
AAGAAGAGCA GGCAAGTGCT GGGTAATCTG GACCTTCAGA GTAAGGAGCT TAGGGAGAAA 
GAACAAGAGC GAGATGGAGA CAAGAAGAGA AGGAAAGAAG AAAGTGGCCG ACCCCAGAAT 
GAAGGGGAAG CGTGGACTCA GATTAGAGAG AAGGGCAGCA GCTCTGGACA TTCTTCCGAT 
CTCCCATCAG GTTCATCGGC ATTATGTTCG GATGACTTTC AGAAACTGAA AGCACTGACA 
GATGGGCCAC CTAAAGAACT GAAGATTCGT CTCATAAAGG TGGAGAGTGG GGACAGGGAG 
ACATTTATTG CTTCAGAAGT AGAAGAGAAG AGAATTCCTC TGAAAGATAT AACCATAAAG 
AACTCTGCTA GTGAAATTAT CAGAGCCTGC AAGGCAGCTC GTGTGAAGGG GAAATTCAAG 
GAATCCTATC TTCTGGATGC CTTTTCTGTG AAACCTGTTG TGAGGACAGA GCTGCCCATT 
CCTCGTGAGA AACTCAACCC CCCTACACCA AGCATCTATT TGGAAAGTAA AAGAGATGCA 
TTCTCTCCAG TCCTGCTGCA GTTTTGTACA GATCCTAAAA ACCCCATTAC TGTCATCCGG 
GGACTAGCTG GCTCTCTCAG ATTAAATCTT GGTTTGTTTT CCACAAAGTC TTTGGTGGAG 
GCAAATTCAG AGCATGCAGT TGAGGTCCGA ACCCAGGTCC AGCAACCAGC AGATGAGAAC 
TGGGATGCCA GCGGCACCGG ACAGACCTGG CCCTGTGAGA GCAGCAGGTC ACATACAACA 
ATTGCCAAGT ATGCCCAGTA CCAGGCATCT AGCTTCCAAG AGAGCTTACA GGAAGAGAAA 
GGCAGTGACG CTGAGGAGGA TAATGATGAA AAGCCCACCA CTAAAGCGGA AAGTACAACC 
AGTAACTCTT CGGCACCCAA CTCCAGTTCA GAGCAGAAAA CTGTGGGGAA AATAATTAAA 
TTTGGCACAA ACATTGACCT GTCTGATCCC AAAAGATGGA AGCCTCAGCT GCAGGAACTG 
CAGAAGTTGC CAGCATTTAT GCGCGTATCC TCCAGTGGGA ATATGCTAAG CCATGTTGGC 
CACACCATTT TGGGAATGAA TACAGTACAA CTATATATGA AGATTCCTGG ATGCCGCACA 
CCAGGACACC AGGAGAACAA CAACTTCTGT TCTGTTAACA TCAACATTGG ACCTGGAGAC 
TGTGAGTGGT TTGCTGTCCA TGATGACTAC TGGGAGGCCA TCAGTGATTT TTGTCAGAAA 
CATGGAGTGG ACTATTTGAC GGGTTCATGG TGGCCAGTTC TGGATGACTT GTATCGCGCT 
AACATCCCTG TGTACCGTTT CATCCAAAGG CCAGGAGATC TGGTGTGGAT CAACGCAGGC 
ACTGTACACT GGGTTCAGGC CGTGGGCTGG TGCAACAACA TTGCCTGGAA TGTGGGACCC 
GTCAACGCTT ATCAGTACCA GGTGGCTTTA GAAAGATTTG AGTGGAACGA AGTGAAGAAA 
GTGAAATCCA TCGTTCCCAT GATTCATGTG TCCTGGAATG TGGCCCGCAC TGTTAAAGTC 
ACCGACCCAG ACGCTTACAA CATGATCAAA CACTGTCTCC TCCAGTCTAT TAAGCACATT 
CAGATTCTGA GGGATCAGCT GGTGGCAGCA GGAATGAAGA TTTCCTACCA GAGCAGAGTG 
AAGGATGAGC CGGCCTACTA CTGTAACGAG TGTGATGTGG AGGTGTTCAA CCTGCTGTTT 
GTGACTAGTG AGAACGGCAG TCGGAAGATG TACGTGGTGC ACTGTGAGGA CTGTGCACGT 
CAACGCAGCC CAAACCTCTC CAATGTAGTA GTGCTGGAGC AGTACCGCAT GGAGGAGCTC 
ATGAACATGT ACGACTCCTT CAGCCTGGCG GTCAGTCCCA GCTCTCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333455.1
CDS	642..6632
Translation	MHHTAEQFTGCSTRDPFPLDGLNRGPWAPVGSRAWPPPSRCLTGVNQHQLLPHMPSHMTGLNHPSKFLNNGPIHRGDKQDQSQALMPALQRMPPAPPRPWDPTRAGQGYEPLPGDNHNRLRNSYNAGPPSHLTARANQLLKFGAPHPQLATHGSRPIHPLPDLWTPPENQQQPRGTHSNSGQLKRPGPPLGEHSVIQHTPPPSLHRPIEDLPSPSKRKKSSGSEQIAHSAMQRLSGPTVHLNQQPSSHYPPPKPGFWNPLHKVGAPWNPTEHKSGPIHFQDSAKPEVESIPYKAPPISLSTLSSPTINTTSGYGQSRASSSQSQKATQSPQSLGPPLQSPHIHHSAYSYSNNRPSDQSQGEPHGTTPQRGHHLDTPPPLIPKFSASLRADRERPAPSPANHTTVPYSHPQFQPHPGLVHSTSPPASNQAKATVPQHNLHKPWRNQESHDCAGTGVSQIAARHSQAHNSVVGNQGPRTPQHVSPPQASARKPVITPNLETPHPPCSLKPYTSVGSIPTMSSAPLTTSSIHSTLSIGRPTDSSVLMSNPNHLSMIGQNPPHLGYQGVHPGVGTLPHLHGVQSQIQQSTLTHTGQSRPNYTPVSSTPSTLNSGLQRSGDSVITSKPSNTLPQPVSSPLSCPHPPAQIKANSILQPMSSRSYNSVTTESSSSISQATTTVPTPVYPGSSLQPTVPCSIADAFNKLDAELQGHMQAEERKRQQEEKRKQIVEKQEVNTKPHCESAIRSLERLLSGNTTEPPPPRLSPANAPSSISPSSQNSPPYPWLSRGGVPPRPSGISATIIEQSQPPPLTPQTEYAREKQRQREKLKSGTPSQNSTGISTYHSESTLISTSNHNMQLKPDPSKDFMLKTAQNIGIIGAIPNPPPLREQPKLYQAFHKDANPSCTLISMTENNLQKQIPSAGLQGLGSNASSCSGDSDGAQFEEETSELLPDGLANIMKMLDESIKKEEELYSGQKGEQAVSELQFSTNVAPVKSYLCAPDLVPALKQSPAEDYQPDGHASPPVLSRQGSLASPCSRTSSLEEEEEVLASSLKVIPKSANSNSVLSQDGSLATTGTNYRHSDLAKLYGISEVVKSECEDDEDEVDREDETPSCSPPQRPHLHQTGVNSMFKNLASVLESQKYTYRGGPFGRPPRSAFVGVKYSSSLSLGPDICRQQQSTSPTSGSASHPGFSSPTQTSAISNSGQTCPLSPTSCESESKRGDKISPSDLLGNDDEKDISEEPKVKVDSGNMKDLLERRPKLTTISESSLAELGQSLEVNKHIIPFKDHSKAEAREICKPVEEKERHRDRERERKHKHSSSSKKHEDKKERKKKHRERHDDASLSSSSSSSSSRRHRDGKSHKEKKSRQVLGNLDLQSKELREKEQERDGDKKRRKEESGRPQNEGEAWTQIREKGSSSGHSSDLPSGSSALCSDDFQKLKALTDGPPKELKIRLIKVESGDRETFIASEVEEKRIPLKDITIKNSASEIIRACKAARVKGKFKESYLLDAFSVKPVVRTELPIPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPKNPITVIRGLAGSLRLNLGLFSTKSLVEANSEHAVEVRTQVQQPADENWDASGTGQTWPCESSRSHTTIAKYAQYQASSFQESLQEEKGSDAEEDNDEKPTTKAESTTSNSSAPNSSSEQKTVGKIIKFGTNIDLSDPKRWKPQLQELQKLPAFMRVSSSGNMLSHVGHTILGMNTVQLYMKIPGCRTPGHQENNNFCSVNINIGPGDCEWFAVHDDYWEAISDFCQKHGVDYLTGSWWPVLDDLYRANIPVYRFIQRPGDLVWINAGTVHWVQAVGWCNNIAWNVGPVNAYQYQVALERFEWNEVKKVKSIVPMIHVSWNVARTVKVTDPDAYNMIKHCLLQSIKHIQILRDQLVAAGMKISYQSRVKDEPAYYCNECDVEVFNLLFVTSENGSRKMYVVHCEDCARQRSPNLSNVVVLEQYRMEELMNMYDSFSLAVSPSSR

Target ORF information:

RefSeq Version	XM_021477780.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 6B, a (kdm6ba), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477780.1

ORF Insert Sequence:

ATGCATCACA CAGCAGAGCA GTTCACCGGG TGCAGCACAC GGGACCCTTT TCCTCTGGAC 
GGACTCAACC GGGGACCATG GGCTCCCGTG GGCAGCCGTG CCTGGCCACC TCCTTCCAGG 
TGCTTAACTG GTGTCAATCA GCACCAGCTT CTTCCCCATA TGCCCAGTCA CATGACTGGG 
CTAAATCATC CTAGTAAATT CTTGAACAAT GGACCCATAC ATCGAGGGGA TAAGCAAGAC 
CAGTCTCAGG CTTTGATGCC GGCATTGCAA AGAATGCCCC CTGCTCCTCC CAGGCCTTGG 
GATCCAACCA GAGCTGGCCA AGGGTATGAG CCATTGCCTG GTGATAACCA CAACAGATTG 
CGCAATAGCT ACAATGCAGG GCCTCCTTCA CACCTTACAG CTCGAGCCAA TCAGCTACTG 
AAGTTTGGTG CTCCTCATCC TCAATTGGCA ACCCATGGAT CACGGCCCAT TCATCCATTA 
CCTGATTTAT GGACTCCACC TGAAAATCAG CAGCAACCCA GGGGAACTCA CTCTAATTCT 
GGACAATTAA AACGTCCTGG TCCCCCTCTC GGAGAACATT CTGTTATTCA GCATACACCT 
CCTCCCTCTC TACACCGGCC CATAGAAGAC CTCCCCAGTC CGAGCAAGAG GAAGAAGAGC 
TCTGGGTCTG AACAGATTGC GCATTCAGCC ATGCAACGTT TATCTGGACC AACTGTACAT 
TTGAATCAAC AGCCATCCTC CCACTATCCC CCACCTAAAC CTGGCTTTTG GAACCCTTTG 
CACAAAGTGG GAGCACCCTG GAACCCAACT GAACACAAAA GTGGACCCAT TCATTTCCAG 
GATTCAGCTA AGCCAGAAGT TGAAAGTATC CCCTATAAAG CACCTCCTAT AAGTCTATCA 
ACCTTATCTT CTCCCACCAT TAACACCACA AGTGGCTATG GACAAAGCAG GGCCTCTTCA 
TCACAGTCAC AAAAGGCCAC TCAATCACCA CAGTCTCTGG GACCACCTTT ACAGAGCCCT 
CACATCCACC ATTCAGCATA CTCTTATTCC AACAACAGAC CTTCAGACCA GTCCCAAGGA 
GAACCACATG GTACTACTCC ACAAAGAGGA CATCATTTGG ATACCCCACC TCCGCTAATA 
CCTAAATTCT CAGCATCACT GCGGGCAGAT AGGGAGCGTC CTGCACCCTC ACCAGCAAAC 
CACACCACTG TGCCTTACAG TCACCCCCAG TTCCAGCCTC ACCCAGGGCT AGTCCATTCC 
ACCAGCCCAC CAGCCAGCAA CCAGGCCAAG GCCACAGTAC CTCAGCACAA CCTCCACAAA 
CCCTGGAGGA ACCAGGAGTC ACATGACTGT GCAGGAACAG GAGTTTCTCA GATCGCAGCA 
CGCCACTCTC AGGCACATAA TAGTGTTGTG GGTAACCAGG GTCCAAGGAC CCCCCAACAT 
GTGAGCCCAC CTCAGGCTTC TGCTCGAAAA CCTGTCATTA CTCCAAACCT AGAAACTCCT 
CATCCACCTT GCTCCCTGAA ACCATATACT AGTGTTGGAA GCATCCCTAC GATGAGCTCG 
GCACCCCTTA CAACCTCCTC AATCCACAGC ACTCTAAGCA TTGGAAGACC AACGGATTCT 
TCAGTGCTGA TGTCAAACCC AAACCATCTA TCCATGATTG GTCAAAATCC ACCACATCTT 
GGTTATCAGG GTGTTCACCC TGGTGTTGGA ACCCTTCCAC ATCTACATGG AGTGCAATCT 
CAAATTCAGC AGTCCACTCT TACCCACACA GGACAAAGTA GACCTAACTA CACCCCAGTG 
TCCTCTACTC CTTCCACCCT AAACTCAGGG CTTCAGAGAT CAGGGGACAG CGTCATCACC 
AGCAAACCCT CTAACACTCT CCCACAACCG GTCAGTTCAC CTCTAAGTTG CCCTCACCCT 
CCAGCACAAA TAAAGGCCAA CTCAATCTTG CAGCCAATGT CCAGCAGGTC ATACAACTCT 
GTAACCACTG AATCTTCCTC CTCAATTTCT CAGGCAACTA CTACTGTTCC AACTCCAGTT 
TACCCAGGAT CCAGTTTGCA ACCTACTGTC CCATGCTCAA TTGCTGATGC TTTTAATAAA 
CTTGATGCTG AGTTGCAGGG CCACATGCAA GCAGAAGAGA GAAAGAGACA ACAGGAAGAA 
AAGAGAAAAC AGATTGTAGA GAAACAAGAA GTGAACACAA AACCACATTG TGAGTCTGCC 
ATTAGAAGTC TGGAACGTTT GTTGTCAGGT AATACAACTG AGCCTCCCCC TCCTCGTTTG 
TCTCCAGCCA ATGCACCCTC TTCCATCTCG CCTTCTAGCC AGAATTCACC ACCTTATCCC 
TGGCTGAGTA GAGGAGGAGT GCCCCCTCGT CCTTCTGGAA TTTCAGCAAC CATTATAGAA 
CAATCACAAC CACCTCCTCT TACTCCCCAG ACAGAATATG CCCGTGAGAA GCAAAGGCAG 
AGGGAGAAAT TGAAGAGTGG GACACCATCT CAGAATTCTA CTGGGATTTC CACATACCAC 
TCAGAATCTA CTCTCATCAG CACCTCCAAC CACAACATGC AACTGAAACC TGACCCATCC 
AAAGACTTCA TGTTGAAAAC CGCTCAAAAT ATTGGGATAA TTGGTGCTAT TCCTAACCCT 
CCACCCCTGC GAGAGCAACC CAAACTTTAT CAAGCTTTTC ACAAGGATGC TAACCCTTCA 
TGCACATTAA TTAGCATGAC GGAAAACAAC CTTCAAAAAC AAATTCCCAG TGCAGGACTT 
CAAGGTCTGG GCAGCAATGC TAGTAGCTGC AGTGGTGATT CAGATGGTGC CCAATTTGAG 
GAAGAAACTT CTGAGCTTTT GCCTGATGGA TTAGCTAACA TTATGAAGAT GCTGGATGAG 
TCAATCAAGA AAGAGGAGGA GTTGTATTCT GGTCAGAAGG GAGAACAAGC AGTGTCTGAA 
CTTCAATTTT CTACAAATGT GGCTCCTGTG AAGAGTTATT TGTGCGCACC AGACCTTGTG 
CCTGCCCTAA AGCAATCACC AGCTGAGGAT TATCAGCCAG ATGGCCATGC AAGCCCCCCT 
GTGTTAAGTC GACAAGGTTC ACTGGCATCT CCTTGCAGCC GCACATCCTC ACTTGAGGAA 
GAAGAAGAGG TTCTTGCTTC TTCACTCAAG GTCATTCCCA AGTCTGCAAA TTCCAATAGT 
GTTCTGTCCC AGGATGGCAG TCTTGCCACA ACAGGAACCA ACTATCGCCA TAGTGACCTG 
GCCAAGCTTT ATGGCATTTC AGAGGTGGTG AAAAGTGAGT GCGAGGATGA TGAAGATGAA 
GTGGATCGAG AAGATGAAAC TCCATCCTGT TCACCACCTC AGCGCCCCCA CCTTCATCAG 
ACAGGTGTGA ACAGCATGTT TAAAAACCTT GCATCTGTAC TTGAAAGTCA GAAGTACACA 
TACCGGGGTG GTCCCTTTGG ACGTCCTCCT CGTTCTGCTT TTGTGGGAGT GAAGTACTCC 
TCATCTCTTT CTTTGGGGCC TGATATATGC AGGCAGCAAC AAAGCACTTC TCCCACATCA 
GGTTCAGCCA GTCACCCAGG CTTTAGCAGT CCAACGCAAA CCTCTGCCAT TAGCAACTCA 
GGTCAGACCT GTCCCCTCTC ACCTACTAGT TGTGAATCAG AGAGTAAAAG AGGGGATAAG 
ATAAGCCCTT CAGATCTTCT GGGCAATGAT GATGAGAAGG ATATTTCAGA AGAACCCAAA 
GTTAAGGTGG ATTCTGGAAA CATGAAAGAC TTGTTGGAAA GACGACCAAA GCTGACCACC 
ATCTCAGAGT CTTCTCTAGC AGAGCTTGGT CAAAGCTTGG AAGTGAATAA ACACATAATC 
CCTTTTAAAG ATCACTCAAA AGCAGAGGCT CGTGAGATAT GCAAACCTGT TGAAGAAAAG 
GAACGACACA GAGACAGAGA ACGAGAAAGG AAACACAAAC ACAGCAGCAG TAGCAAAAAA 
CATGAGGACA AGAAAGAGAG AAAGAAAAAG CACAGAGAAA GACATGACGA TGCATCCCTT 
TCCTCCTCCT CTTCTTCCAG CTCCAGTCGA AGACACAGGG ACGGAAAGTC ACATAAGGAA 
AAGAAGAGCA GGCAAGTGCT GGGTAATCTG GACCTTCAGA GTAAGGAGCT TAGGGAGAAA 
GAACAAGAGC GAGATGGAGA CAAGAAGAGA AGGAAAGAAG AAAGTGGCCG ACCCCAGAAT 
GAAGGGGAAG CGTGGACTCA GATTAGAGAG AAGGGCAGCA GCTCTGGACA TTCTTCCGAT 
CTCCCATCAG GTTCATCGGC ATTATGTTCG GATGACTTTC AGAAACTGAA AGCACTGACA 
GATGGGCCAC CTAAAGAACT GAAGATTCGT CTCATAAAGG TGGAGAGTGG GGACAGGGAG 
ACATTTATTG CTTCAGAAGT AGAAGAGAAG AGAATTCCTC TGAAAGATAT AACCATAAAG 
AACTCTGCTA GTGAAATTAT CAGAGCCTGC AAGGCAGCTC GTGTGAAGGG GAAATTCAAG 
GAATCCTATC TTCTGGATGC CTTTTCTGTG AAACCTGTTG TGAGGACAGA GCTGCCCATT 
CCTCGTGAGA AACTCAACCC CCCTACACCA AGCATCTATT TGGAAAGTAA AAGAGATGCA 
TTCTCTCCAG TCCTGCTGCA GTTTTGTACA GATCCTAAAA ACCCCATTAC TGTCATCCGG 
GGACTAGCTG GCTCTCTCAG ATTAAATCTT GGTTTGTTTT CCACAAAGTC TTTGGTGGAG 
GCAAATTCAG AGCATGCAGT TGAGGTCCGA ACCCAGGTCC AGCAACCAGC AGATGAGAAC 
TGGGATGCCA GCGGCACCGG ACAGACCTGG CCCTGTGAGA GCAGCAGGTC ACATACAACA 
ATTGCCAAGT ATGCCCAGTA CCAGGCATCT AGCTTCCAAG AGAGCTTACA GGAAGAGAAA 
GGCAGTGACG CTGAGGAGGA TAATGATGAA AAGCCCACCA CTAAAGCGGA AAGTACAACC 
AGTAACTCTT CGGCACCCAA CTCCAGTTCA GAGCAGAAAA CTGTGGGGAA AATAATTAAA 
TTTGGCACAA ACATTGACCT GTCTGATCCC AAAAGATGGA AGCCTCAGCT GCAGGAACTG 
CAGAAGTTGC CAGCATTTAT GCGCGTATCC TCCAGTGGGA ATATGCTAAG CCATGTTGGC 
CACACCATTT TGGGAATGAA TACAGTACAA CTATATATGA AGATTCCTGG ATGCCGCACA 
CCAGGACACC AGGAGAACAA CAACTTCTGT TCTGTTAACA TCAACATTGG ACCTGGAGAC 
TGTGAGTGGT TTGCTGTCCA TGATGACTAC TGGGAGGCCA TCAGTGATTT TTGTCAGAAA 
CATGGAGTGG ACTATTTGAC GGGTTCATGG TGGCCAGTTC TGGATGACTT GTATCGCGCT 
AACATCCCTG TGTACCGTTT CATCCAAAGG CCAGGAGATC TGGTGTGGAT CAACGCAGGC 
ACTGTACACT GGGTTCAGGC CGTGGGCTGG TGCAACAACA TTGCCTGGAA TGTGGGACCC 
GTCAACGCTT ATCAGTACCA GGTGGCTTTA GAAAGATTTG AGTGGAACGA AGTGAAGAAA 
GTGAAATCCA TCGTTCCCAT GATTCATGTG TCCTGGAATG TGGCCCGCAC TGTTAAAGTC 
ACCGACCCAG ACGCTTACAA CATGATCAAA CACTGTCTCC TCCAGTCTAT TAAGCACATT 
CAGATTCTGA GGGATCAGCT GGTGGCAGCA GGAATGAAGA TTTCCTACCA GAGCAGAGTG 
AAGGATGAGC CGGCCTACTA CTGTAACGAG TGTGATGTGG AGGTGTTCAA CCTGCTGTTT 
GTGACTAGTG AGAACGGCAG TCGGAAGATG TACGTGGTGC ACTGTGAGGA CTGTGCACGT 
CAACGCAGCC CAAACCTCTC CAATGTAGTA GTGCTGGAGC AGTACCGCAT GGAGGAGCTC 
ATGAACATGT ACGACTCCTT CAGCCTGGCG GTCAGTCCCA GCTCTCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6BA ( XM_009303125.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009301400.1 Danio rerio lysine (K)-specific demethylase 6B, a (kdm6ba), transcript variant X1, mRNA.

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CloneID	ODa12856
Clone ID Related Accession (Same CDS sequence)	XM_005172239.4 , XM_021477779.1 , XM_021477780.1 , XM_009303125.2 , XM_005172240.3 , XM_017357011.1 , XM_005172240.4 , XM_009303125.3 , XM_005172239.3
Accession Version	XM_009303125.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	lysine-specific demethylase 6B
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303125.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCACA CAGCAGAGCA GTTCACCGGG TGCAGCACAC GGGACCCTTT TCCTCTGGAC 
GGACTCAACC GGGGACCATG GGCTCCCGTG GGCAGCCGTG CCTGGCCACC TCCTTCCAGG 
TGCTTAACTG GTGTCAATCA GCACCAGCTT CTTCCCCATA TGCCCAGTCA CATGACTGGG 
CTAAATCATC CTAGTAAATT CTTGAACAAT GGACCCATAC ATCGAGGGGA TAAGCAAGAC 
CAGTCTCAGG CTTTGATGCC GGCATTGCAA AGAATGCCCC CTGCTCCTCC CAGGCCTTGG 
GATCCAACCA GAGCTGGCCA AGGGTATGAG CCATTGCCTG GTGATAACCA CAACAGATTG 
CGCAATAGCT ACAATGCAGG GCCTCCTTCA CACCTTACAG CTCGAGCCAA TCAGCTACTG 
AAGTTTGGTG CTCCTCATCC TCAATTGGCA ACCCATGGAT CACGGCCCAT TCATCCATTA 
CCTGATTTAT GGACTCCACC TGAAAATCAG CAGCAACCCA GGGGAACTCA CTCTAATTCT 
GGACAATTAA AACGTCCTGG TCCCCCTCTC GGAGAACATT CTGTTATTCA GCATACACCT 
CCTCCCTCTC TACACCGGCC CATAGAAGAC CTCCCCAGTC CGAGCAAGAG GAAGAAGAGC 
TCTGGGTCTG AACAGATTGC GCATTCAGCC ATGCAACGTT TATCTGGACC AACTGTACAT 
TTGAATCAAC AGCCATCCTC CCACTATCCC CCACCTAAAC CTGGCTTTTG GAACCCTTTG 
CACAAAGTGG GAGCACCCTG GAACCCAACT GAACACAAAA GTGGACCCAT TCATTTCCAG 
GATTCAGCTA AGCCAGAAGT TGAAAGTATC CCCTATAAAG CACCTCCTAT AAGTCTATCA 
ACCTTATCTT CTCCCACCAT TAACACCACA AGTGGCTATG GACAAAGCAG GGCCTCTTCA 
TCACAGTCAC AAAAGGCCAC TCAATCACCA CAGTCTCTGG GACCACCTTT ACAGAGCCCT 
CACATCCACC ATTCAGCATA CTCTTATTCC AACAACAGAC CTTCAGACCA GTCCCAAGGA 
GAACCACATG GTACTACTCC ACAAAGAGGA CATCATTTGG ATACCCCACC TCCGCTAATA 
CCTAAATTCT CAGCATCACT GCGGGCAGAT AGGGAGCGTC CTGCACCCTC ACCAGCAAAC 
CACACCACTG TGCCTTACAG TCACCCCCAG TTCCAGCCTC ACCCAGGGCT AGTCCATTCC 
ACCAGCCCAC CAGCCAGCAA CCAGGCCAAG GCCACAGTAC CTCAGCACAA CCTCCACAAA 
CCCTGGAGGA ACCAGGAGTC ACATGACTGT GCAGGAACAG GAGTTTCTCA GATCGCAGCA 
CGCCACTCTC AGGCACATAA TAGTGTTGTG GGTAACCAGG GTCCAAGGAC CCCCCAACAT 
GTGAGCCCAC CTCAGGCTTC TGCTCGAAAA CCTGTCATTA CTCCAAACCT AGAAACTCCT 
CATCCACCTT GCTCCCTGAA ACCATATACT AGTGTTGGAA GCATCCCTAC GATGAGCTCG 
GCACCCCTTA CAACCTCCTC AATCCACAGC ACTCTAAGCA TTGGAAGACC AACGGATTCT 
TCAGTGCTGA TGTCAAACCC AAACCATCTA TCCATGATTG GTCAAAATCC ACCACATCTT 
GGTTATCAGG GTGTTCACCC TGGTGTTGGA ACCCTTCCAC ATCTACATGG AGTGCAATCT 
CAAATTCAGC AGTCCACTCT TACCCACACA GGACAAAGTA GACCTAACTA CACCCCAGTG 
TCCTCTACTC CTTCCACCCT AAACTCAGGG CTTCAGAGAT CAGGGGACAG CGTCATCACC 
AGCAAACCCT CTAACACTCT CCCACAACCG GTCAGTTCAC CTCTAAGTTG CCCTCACCCT 
CCAGCACAAA TAAAGGCCAA CTCAATCTTG CAGCCAATGT CCAGCAGGTC ATACAACTCT 
GTAACCACTG AATCTTCCTC CTCAATTTCT CAGGCAACTA CTACTGTTCC AACTCCAGTT 
TACCCAGGAT CCAGTTTGCA ACCTACTGTC CCATGCTCAA TTGCTGATGC TTTTAATAAA 
CTTGATGCTG AGTTGCAGGG CCACATGCAA GCAGAAGAGA GAAAGAGACA ACAGGAAGAA 
AAGAGAAAAC AGATTGTAGA GAAACAAGAA GTGAACACAA AACCACATTG TGAGTCTGCC 
ATTAGAAGTC TGGAACGTTT GTTGTCAGGT AATACAACTG AGCCTCCCCC TCCTCGTTTG 
TCTCCAGCCA ATGCACCCTC TTCCATCTCG CCTTCTAGCC AGAATTCACC ACCTTATCCC 
TGGCTGAGTA GAGGAGGAGT GCCCCCTCGT CCTTCTGGAA TTTCAGCAAC CATTATAGAA 
CAATCACAAC CACCTCCTCT TACTCCCCAG ACAGAATATG CCCGTGAGAA GCAAAGGCAG 
AGGGAGAAAT TGAAGAGTGG GACACCATCT CAGAATTCTA CTGGGATTTC CACATACCAC 
TCAGAATCTA CTCTCATCAG CACCTCCAAC CACAACATGC AACTGAAACC TGACCCATCC 
AAAGACTTCA TGTTGAAAAC CGCTCAAAAT ATTGGGATAA TTGGTGCTAT TCCTAACCCT 
CCACCCCTGC GAGAGCAACC CAAACTTTAT CAAGCTTTTC ACAAGGATGC TAACCCTTCA 
TGCACATTAA TTAGCATGAC GGAAAACAAC CTTCAAAAAC AAATTCCCAG TGCAGGACTT 
CAAGGTCTGG GCAGCAATGC TAGTAGCTGC AGTGGTGATT CAGATGGTGC CCAATTTGAG 
GAAGAAACTT CTGAGCTTTT GCCTGATGGA TTAGCTAACA TTATGAAGAT GCTGGATGAG 
TCAATCAAGA AAGAGGAGGA GTTGTATTCT GGTCAGAAGG GAGAACAAGC AGTGTCTGAA 
CTTCAATTTT CTACAAATGT GGCTCCTGTG AAGAGTTATT TGTGCGCACC AGACCTTGTG 
CCTGCCCTAA AGCAATCACC AGCTGAGGAT TATCAGCCAG ATGGCCATGC AAGCCCCCCT 
GTGTTAAGTC GACAAGGTTC ACTGGCATCT CCTTGCAGCC GCACATCCTC ACTTGAGGAA 
GAAGAAGAGG TTCTTGCTTC TTCACTCAAG GTCATTCCCA AGTCTGCAAA TTCCAATAGT 
GTTCTGTCCC AGGATGGCAG TCTTGCCACA ACAGGAACCA ACTATCGCCA TAGTGACCTG 
GCCAAGCTTT ATGGCATTTC AGAGGTGGTG AAAAGTGAGT GCGAGGATGA TGAAGATGAA 
GTGGATCGAG AAGATGAAAC TCCATCCTGT TCACCACCTC AGCGCCCCCA CCTTCATCAG 
ACAGGTGTGA ACAGCATGTT TAAAAACCTT GCATCTGTAC TTGAAAGTCA GAAGTACACA 
TACCGGGGTG GTCCCTTTGG ACGTCCTCCT CGTTCTGCTT TTGTGGGAGT GAAGTACTCC 
TCATCTCTTT CTTTGGGGCC TGATATATGC AGGCAGCAAC AAAGCACTTC TCCCACATCA 
GGTTCAGCCA GTCACCCAGG CTTTAGCAGT CCAACGCAAA CCTCTGCCAT TAGCAACTCA 
GGTCAGACCT GTCCCCTCTC ACCTACTAGT TGTGAATCAG AGAGTAAAAG AGGGGATAAG 
ATAAGCCCTT CAGATCTTCT GGGCAATGAT GATGAGAAGG ATATTTCAGA AGAACCCAAA 
GTTAAGGTGG ATTCTGGAAA CATGAAAGAC TTGTTGGAAA GACGACCAAA GCTGACCACC 
ATCTCAGAGT CTTCTCTAGC AGAGCTTGGT CAAAGCTTGG AAGTGAATAA ACACATAATC 
CCTTTTAAAG ATCACTCAAA AGCAGAGGCT CGTGAGATAT GCAAACCTGT TGAAGAAAAG 
GAACGACACA GAGACAGAGA ACGAGAAAGG AAACACAAAC ACAGCAGCAG TAGCAAAAAA 
CATGAGGACA AGAAAGAGAG AAAGAAAAAG CACAGAGAAA GACATGACGA TGCATCCCTT 
TCCTCCTCCT CTTCTTCCAG CTCCAGTCGA AGACACAGGG ACGGAAAGTC ACATAAGGAA 
AAGAAGAGCA GGCAAGTGCT GGGTAATCTG GACCTTCAGA GTAAGGAGCT TAGGGAGAAA 
GAACAAGAGC GAGATGGAGA CAAGAAGAGA AGGAAAGAAG AAAGTGGCCG ACCCCAGAAT 
GAAGGGGAAG CGTGGACTCA GATTAGAGAG AAGGGCAGCA GCTCTGGACA TTCTTCCGAT 
CTCCCATCAG GTTCATCGGC ATTATGTTCG GATGACTTTC AGAAACTGAA AGCACTGACA 
GATGGGCCAC CTAAAGAACT GAAGATTCGT CTCATAAAGG TGGAGAGTGG GGACAGGGAG 
ACATTTATTG CTTCAGAAGT AGAAGAGAAG AGAATTCCTC TGAAAGATAT AACCATAAAG 
AACTCTGCTA GTGAAATTAT CAGAGCCTGC AAGGCAGCTC GTGTGAAGGG GAAATTCAAG 
GAATCCTATC TTCTGGATGC CTTTTCTGTG AAACCTGTTG TGAGGACAGA GCTGCCCATT 
CCTCGTGAGA AACTCAACCC CCCTACACCA AGCATCTATT TGGAAAGTAA AAGAGATGCA 
TTCTCTCCAG TCCTGCTGCA GTTTTGTACA GATCCTAAAA ACCCCATTAC TGTCATCCGG 
GGACTAGCTG GCTCTCTCAG ATTAAATCTT GGTTTGTTTT CCACAAAGTC TTTGGTGGAG 
GCAAATTCAG AGCATGCAGT TGAGGTCCGA ACCCAGGTCC AGCAACCAGC AGATGAGAAC 
TGGGATGCCA GCGGCACCGG ACAGACCTGG CCCTGTGAGA GCAGCAGGTC ACATACAACA 
ATTGCCAAGT ATGCCCAGTA CCAGGCATCT AGCTTCCAAG AGAGCTTACA GGAAGAGAAA 
GGCAGTGACG CTGAGGAGGA TAATGATGAA AAGCCCACCA CTAAAGCGGA AAGTACAACC 
AGTAACTCTT CGGCACCCAA CTCCAGTTCA GAGCAGAAAA CTGTGGGGAA AATAATTAAA 
TTTGGCACAA ACATTGACCT GTCTGATCCC AAAAGATGGA AGCCTCAGCT GCAGGAACTG 
CAGAAGTTGC CAGCATTTAT GCGCGTATCC TCCAGTGGGA ATATGCTAAG CCATGTTGGC 
CACACCATTT TGGGAATGAA TACAGTACAA CTATATATGA AGATTCCTGG ATGCCGCACA 
CCAGGACACC AGGAGAACAA CAACTTCTGT TCTGTTAACA TCAACATTGG ACCTGGAGAC 
TGTGAGTGGT TTGCTGTCCA TGATGACTAC TGGGAGGCCA TCAGTGATTT TTGTCAGAAA 
CATGGAGTGG ACTATTTGAC GGGTTCATGG TGGCCAGTTC TGGATGACTT GTATCGCGCT 
AACATCCCTG TGTACCGTTT CATCCAAAGG CCAGGAGATC TGGTGTGGAT CAACGCAGGC 
ACTGTACACT GGGTTCAGGC CGTGGGCTGG TGCAACAACA TTGCCTGGAA TGTGGGACCC 
GTCAACGCTT ATCAGTACCA GGTGGCTTTA GAAAGATTTG AGTGGAACGA AGTGAAGAAA 
GTGAAATCCA TCGTTCCCAT GATTCATGTG TCCTGGAATG TGGCCCGCAC TGTTAAAGTC 
ACCGACCCAG ACGCTTACAA CATGATCAAA CACTGTCTCC TCCAGTCTAT TAAGCACATT 
CAGATTCTGA GGGATCAGCT GGTGGCAGCA GGAATGAAGA TTTCCTACCA GAGCAGAGTG 
AAGGATGAGC CGGCCTACTA CTGTAACGAG TGTGATGTGG AGGTGTTCAA CCTGCTGTTT 
GTGACTAGTG AGAACGGCAG TCGGAAGATG TACGTGGTGC ACTGTGAGGA CTGTGCACGT 
CAACGCAGCC CAAACCTCTC CAATGTAGTA GTGCTGGAGC AGTACCGCAT GGAGGAGCTC 
ATGAACATGT ACGACTCCTT CAGCCTGGCG GTCAGTCCCA GCTCTCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301400.1
CDS	1017..7007
Translation	MHHTAEQFTGCSTRDPFPLDGLNRGPWAPVGSRAWPPPSRCLTGVNQHQLLPHMPSHMTGLNHPSKFLNNGPIHRGDKQDQSQALMPALQRMPPAPPRPWDPTRAGQGYEPLPGDNHNRLRNSYNAGPPSHLTARANQLLKFGAPHPQLATHGSRPIHPLPDLWTPPENQQQPRGTHSNSGQLKRPGPPLGEHSVIQHTPPPSLHRPIEDLPSPSKRKKSSGSEQIAHSAMQRLSGPTVHLNQQPSSHYPPPKPGFWNPLHKVGAPWNPTEHKSGPIHFQDSAKPEVESIPYKAPPISLSTLSSPTINTTSGYGQSRASSSQSQKATQSPQSLGPPLQSPHIHHSAYSYSNNRPSDQSQGEPHGTTPQRGHHLDTPPPLIPKFSASLRADRERPAPSPANHTTVPYSHPQFQPHPGLVHSTSPPASNQAKATVPQHNLHKPWRNQESHDCAGTGVSQIAARHSQAHNSVVGNQGPRTPQHVSPPQASARKPVITPNLETPHPPCSLKPYTSVGSIPTMSSAPLTTSSIHSTLSIGRPTDSSVLMSNPNHLSMIGQNPPHLGYQGVHPGVGTLPHLHGVQSQIQQSTLTHTGQSRPNYTPVSSTPSTLNSGLQRSGDSVITSKPSNTLPQPVSSPLSCPHPPAQIKANSILQPMSSRSYNSVTTESSSSISQATTTVPTPVYPGSSLQPTVPCSIADAFNKLDAELQGHMQAEERKRQQEEKRKQIVEKQEVNTKPHCESAIRSLERLLSGNTTEPPPPRLSPANAPSSISPSSQNSPPYPWLSRGGVPPRPSGISATIIEQSQPPPLTPQTEYAREKQRQREKLKSGTPSQNSTGISTYHSESTLISTSNHNMQLKPDPSKDFMLKTAQNIGIIGAIPNPPPLREQPKLYQAFHKDANPSCTLISMTENNLQKQIPSAGLQGLGSNASSCSGDSDGAQFEEETSELLPDGLANIMKMLDESIKKEEELYSGQKGEQAVSELQFSTNVAPVKSYLCAPDLVPALKQSPAEDYQPDGHASPPVLSRQGSLASPCSRTSSLEEEEEVLASSLKVIPKSANSNSVLSQDGSLATTGTNYRHSDLAKLYGISEVVKSECEDDEDEVDREDETPSCSPPQRPHLHQTGVNSMFKNLASVLESQKYTYRGGPFGRPPRSAFVGVKYSSSLSLGPDICRQQQSTSPTSGSASHPGFSSPTQTSAISNSGQTCPLSPTSCESESKRGDKISPSDLLGNDDEKDISEEPKVKVDSGNMKDLLERRPKLTTISESSLAELGQSLEVNKHIIPFKDHSKAEAREICKPVEEKERHRDRERERKHKHSSSSKKHEDKKERKKKHRERHDDASLSSSSSSSSSRRHRDGKSHKEKKSRQVLGNLDLQSKELREKEQERDGDKKRRKEESGRPQNEGEAWTQIREKGSSSGHSSDLPSGSSALCSDDFQKLKALTDGPPKELKIRLIKVESGDRETFIASEVEEKRIPLKDITIKNSASEIIRACKAARVKGKFKESYLLDAFSVKPVVRTELPIPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPKNPITVIRGLAGSLRLNLGLFSTKSLVEANSEHAVEVRTQVQQPADENWDASGTGQTWPCESSRSHTTIAKYAQYQASSFQESLQEEKGSDAEEDNDEKPTTKAESTTSNSSAPNSSSEQKTVGKIIKFGTNIDLSDPKRWKPQLQELQKLPAFMRVSSSGNMLSHVGHTILGMNTVQLYMKIPGCRTPGHQENNNFCSVNINIGPGDCEWFAVHDDYWEAISDFCQKHGVDYLTGSWWPVLDDLYRANIPVYRFIQRPGDLVWINAGTVHWVQAVGWCNNIAWNVGPVNAYQYQVALERFEWNEVKKVKSIVPMIHVSWNVARTVKVTDPDAYNMIKHCLLQSIKHIQILRDQLVAAGMKISYQSRVKDEPAYYCNECDVEVFNLLFVTSENGSRKMYVVHCEDCARQRSPNLSNVVVLEQYRMEELMNMYDSFSLAVSPSSR

Target ORF information:

RefSeq Version	XM_009303125.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 6B, a (kdm6ba), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303125.2

ORF Insert Sequence:

ATGCATCACA CAGCAGAGCA GTTCACCGGG TGCAGCACAC GGGACCCTTT TCCTCTGGAC 
GGACTCAACC GGGGACCATG GGCTCCCGTG GGCAGCCGTG CCTGGCCACC TCCTTCCAGG 
TGCTTAACTG GTGTCAATCA GCACCAGCTT CTTCCCCATA TGCCCAGTCA CATGACTGGG 
CTAAATCATC CTAGTAAATT CTTGAACAAT GGACCCATAC ATCGAGGGGA TAAGCAAGAC 
CAGTCTCAGG CTTTGATGCC GGCATTGCAA AGAATGCCCC CTGCTCCTCC CAGGCCTTGG 
GATCCAACCA GAGCTGGCCA AGGGTATGAG CCATTGCCTG GTGATAACCA CAACAGATTG 
CGCAATAGCT ACAATGCAGG GCCTCCTTCA CACCTTACAG CTCGAGCCAA TCAGCTACTG 
AAGTTTGGTG CTCCTCATCC TCAATTGGCA ACCCATGGAT CACGGCCCAT TCATCCATTA 
CCTGATTTAT GGACTCCACC TGAAAATCAG CAGCAACCCA GGGGAACTCA CTCTAATTCT 
GGACAATTAA AACGTCCTGG TCCCCCTCTC GGAGAACATT CTGTTATTCA GCATACACCT 
CCTCCCTCTC TACACCGGCC CATAGAAGAC CTCCCCAGTC CGAGCAAGAG GAAGAAGAGC 
TCTGGGTCTG AACAGATTGC GCATTCAGCC ATGCAACGTT TATCTGGACC AACTGTACAT 
TTGAATCAAC AGCCATCCTC CCACTATCCC CCACCTAAAC CTGGCTTTTG GAACCCTTTG 
CACAAAGTGG GAGCACCCTG GAACCCAACT GAACACAAAA GTGGACCCAT TCATTTCCAG 
GATTCAGCTA AGCCAGAAGT TGAAAGTATC CCCTATAAAG CACCTCCTAT AAGTCTATCA 
ACCTTATCTT CTCCCACCAT TAACACCACA AGTGGCTATG GACAAAGCAG GGCCTCTTCA 
TCACAGTCAC AAAAGGCCAC TCAATCACCA CAGTCTCTGG GACCACCTTT ACAGAGCCCT 
CACATCCACC ATTCAGCATA CTCTTATTCC AACAACAGAC CTTCAGACCA GTCCCAAGGA 
GAACCACATG GTACTACTCC ACAAAGAGGA CATCATTTGG ATACCCCACC TCCGCTAATA 
CCTAAATTCT CAGCATCACT GCGGGCAGAT AGGGAGCGTC CTGCACCCTC ACCAGCAAAC 
CACACCACTG TGCCTTACAG TCACCCCCAG TTCCAGCCTC ACCCAGGGCT AGTCCATTCC 
ACCAGCCCAC CAGCCAGCAA CCAGGCCAAG GCCACAGTAC CTCAGCACAA CCTCCACAAA 
CCCTGGAGGA ACCAGGAGTC ACATGACTGT GCAGGAACAG GAGTTTCTCA GATCGCAGCA 
CGCCACTCTC AGGCACATAA TAGTGTTGTG GGTAACCAGG GTCCAAGGAC CCCCCAACAT 
GTGAGCCCAC CTCAGGCTTC TGCTCGAAAA CCTGTCATTA CTCCAAACCT AGAAACTCCT 
CATCCACCTT GCTCCCTGAA ACCATATACT AGTGTTGGAA GCATCCCTAC GATGAGCTCG 
GCACCCCTTA CAACCTCCTC AATCCACAGC ACTCTAAGCA TTGGAAGACC AACGGATTCT 
TCAGTGCTGA TGTCAAACCC AAACCATCTA TCCATGATTG GTCAAAATCC ACCACATCTT 
GGTTATCAGG GTGTTCACCC TGGTGTTGGA ACCCTTCCAC ATCTACATGG AGTGCAATCT 
CAAATTCAGC AGTCCACTCT TACCCACACA GGACAAAGTA GACCTAACTA CACCCCAGTG 
TCCTCTACTC CTTCCACCCT AAACTCAGGG CTTCAGAGAT CAGGGGACAG CGTCATCACC 
AGCAAACCCT CTAACACTCT CCCACAACCG GTCAGTTCAC CTCTAAGTTG CCCTCACCCT 
CCAGCACAAA TAAAGGCCAA CTCAATCTTG CAGCCAATGT CCAGCAGGTC ATACAACTCT 
GTAACCACTG AATCTTCCTC CTCAATTTCT CAGGCAACTA CTACTGTTCC AACTCCAGTT 
TACCCAGGAT CCAGTTTGCA ACCTACTGTC CCATGCTCAA TTGCTGATGC TTTTAATAAA 
CTTGATGCTG AGTTGCAGGG CCACATGCAA GCAGAAGAGA GAAAGAGACA ACAGGAAGAA 
AAGAGAAAAC AGATTGTAGA GAAACAAGAA GTGAACACAA AACCACATTG TGAGTCTGCC 
ATTAGAAGTC TGGAACGTTT GTTGTCAGGT AATACAACTG AGCCTCCCCC TCCTCGTTTG 
TCTCCAGCCA ATGCACCCTC TTCCATCTCG CCTTCTAGCC AGAATTCACC ACCTTATCCC 
TGGCTGAGTA GAGGAGGAGT GCCCCCTCGT CCTTCTGGAA TTTCAGCAAC CATTATAGAA 
CAATCACAAC CACCTCCTCT TACTCCCCAG ACAGAATATG CCCGTGAGAA GCAAAGGCAG 
AGGGAGAAAT TGAAGAGTGG GACACCATCT CAGAATTCTA CTGGGATTTC CACATACCAC 
TCAGAATCTA CTCTCATCAG CACCTCCAAC CACAACATGC AACTGAAACC TGACCCATCC 
AAAGACTTCA TGTTGAAAAC CGCTCAAAAT ATTGGGATAA TTGGTGCTAT TCCTAACCCT 
CCACCCCTGC GAGAGCAACC CAAACTTTAT CAAGCTTTTC ACAAGGATGC TAACCCTTCA 
TGCACATTAA TTAGCATGAC GGAAAACAAC CTTCAAAAAC AAATTCCCAG TGCAGGACTT 
CAAGGTCTGG GCAGCAATGC TAGTAGCTGC AGTGGTGATT CAGATGGTGC CCAATTTGAG 
GAAGAAACTT CTGAGCTTTT GCCTGATGGA TTAGCTAACA TTATGAAGAT GCTGGATGAG 
TCAATCAAGA AAGAGGAGGA GTTGTATTCT GGTCAGAAGG GAGAACAAGC AGTGTCTGAA 
CTTCAATTTT CTACAAATGT GGCTCCTGTG AAGAGTTATT TGTGCGCACC AGACCTTGTG 
CCTGCCCTAA AGCAATCACC AGCTGAGGAT TATCAGCCAG ATGGCCATGC AAGCCCCCCT 
GTGTTAAGTC GACAAGGTTC ACTGGCATCT CCTTGCAGCC GCACATCCTC ACTTGAGGAA 
GAAGAAGAGG TTCTTGCTTC TTCACTCAAG GTCATTCCCA AGTCTGCAAA TTCCAATAGT 
GTTCTGTCCC AGGATGGCAG TCTTGCCACA ACAGGAACCA ACTATCGCCA TAGTGACCTG 
GCCAAGCTTT ATGGCATTTC AGAGGTGGTG AAAAGTGAGT GCGAGGATGA TGAAGATGAA 
GTGGATCGAG AAGATGAAAC TCCATCCTGT TCACCACCTC AGCGCCCCCA CCTTCATCAG 
ACAGGTGTGA ACAGCATGTT TAAAAACCTT GCATCTGTAC TTGAAAGTCA GAAGTACACA 
TACCGGGGTG GTCCCTTTGG ACGTCCTCCT CGTTCTGCTT TTGTGGGAGT GAAGTACTCC 
TCATCTCTTT CTTTGGGGCC TGATATATGC AGGCAGCAAC AAAGCACTTC TCCCACATCA 
GGTTCAGCCA GTCACCCAGG CTTTAGCAGT CCAACGCAAA CCTCTGCCAT TAGCAACTCA 
GGTCAGACCT GTCCCCTCTC ACCTACTAGT TGTGAATCAG AGAGTAAAAG AGGGGATAAG 
ATAAGCCCTT CAGATCTTCT GGGCAATGAT GATGAGAAGG ATATTTCAGA AGAACCCAAA 
GTTAAGGTGG ATTCTGGAAA CATGAAAGAC TTGTTGGAAA GACGACCAAA GCTGACCACC 
ATCTCAGAGT CTTCTCTAGC AGAGCTTGGT CAAAGCTTGG AAGTGAATAA ACACATAATC 
CCTTTTAAAG ATCACTCAAA AGCAGAGGCT CGTGAGATAT GCAAACCTGT TGAAGAAAAG 
GAACGACACA GAGACAGAGA ACGAGAAAGG AAACACAAAC ACAGCAGCAG TAGCAAAAAA 
CATGAGGACA AGAAAGAGAG AAAGAAAAAG CACAGAGAAA GACATGACGA TGCATCCCTT 
TCCTCCTCCT CTTCTTCCAG CTCCAGTCGA AGACACAGGG ACGGAAAGTC ACATAAGGAA 
AAGAAGAGCA GGCAAGTGCT GGGTAATCTG GACCTTCAGA GTAAGGAGCT TAGGGAGAAA 
GAACAAGAGC GAGATGGAGA CAAGAAGAGA AGGAAAGAAG AAAGTGGCCG ACCCCAGAAT 
GAAGGGGAAG CGTGGACTCA GATTAGAGAG AAGGGCAGCA GCTCTGGACA TTCTTCCGAT 
CTCCCATCAG GTTCATCGGC ATTATGTTCG GATGACTTTC AGAAACTGAA AGCACTGACA 
GATGGGCCAC CTAAAGAACT GAAGATTCGT CTCATAAAGG TGGAGAGTGG GGACAGGGAG 
ACATTTATTG CTTCAGAAGT AGAAGAGAAG AGAATTCCTC TGAAAGATAT AACCATAAAG 
AACTCTGCTA GTGAAATTAT CAGAGCCTGC AAGGCAGCTC GTGTGAAGGG GAAATTCAAG 
GAATCCTATC TTCTGGATGC CTTTTCTGTG AAACCTGTTG TGAGGACAGA GCTGCCCATT 
CCTCGTGAGA AACTCAACCC CCCTACACCA AGCATCTATT TGGAAAGTAA AAGAGATGCA 
TTCTCTCCAG TCCTGCTGCA GTTTTGTACA GATCCTAAAA ACCCCATTAC TGTCATCCGG 
GGACTAGCTG GCTCTCTCAG ATTAAATCTT GGTTTGTTTT CCACAAAGTC TTTGGTGGAG 
GCAAATTCAG AGCATGCAGT TGAGGTCCGA ACCCAGGTCC AGCAACCAGC AGATGAGAAC 
TGGGATGCCA GCGGCACCGG ACAGACCTGG CCCTGTGAGA GCAGCAGGTC ACATACAACA 
ATTGCCAAGT ATGCCCAGTA CCAGGCATCT AGCTTCCAAG AGAGCTTACA GGAAGAGAAA 
GGCAGTGACG CTGAGGAGGA TAATGATGAA AAGCCCACCA CTAAAGCGGA AAGTACAACC 
AGTAACTCTT CGGCACCCAA CTCCAGTTCA GAGCAGAAAA CTGTGGGGAA AATAATTAAA 
TTTGGCACAA ACATTGACCT GTCTGATCCC AAAAGATGGA AGCCTCAGCT GCAGGAACTG 
CAGAAGTTGC CAGCATTTAT GCGCGTATCC TCCAGTGGGA ATATGCTAAG CCATGTTGGC 
CACACCATTT TGGGAATGAA TACAGTACAA CTATATATGA AGATTCCTGG ATGCCGCACA 
CCAGGACACC AGGAGAACAA CAACTTCTGT TCTGTTAACA TCAACATTGG ACCTGGAGAC 
TGTGAGTGGT TTGCTGTCCA TGATGACTAC TGGGAGGCCA TCAGTGATTT TTGTCAGAAA 
CATGGAGTGG ACTATTTGAC GGGTTCATGG TGGCCAGTTC TGGATGACTT GTATCGCGCT 
AACATCCCTG TGTACCGTTT CATCCAAAGG CCAGGAGATC TGGTGTGGAT CAACGCAGGC 
ACTGTACACT GGGTTCAGGC CGTGGGCTGG TGCAACAACA TTGCCTGGAA TGTGGGACCC 
GTCAACGCTT ATCAGTACCA GGTGGCTTTA GAAAGATTTG AGTGGAACGA AGTGAAGAAA 
GTGAAATCCA TCGTTCCCAT GATTCATGTG TCCTGGAATG TGGCCCGCAC TGTTAAAGTC 
ACCGACCCAG ACGCTTACAA CATGATCAAA CACTGTCTCC TCCAGTCTAT TAAGCACATT 
CAGATTCTGA GGGATCAGCT GGTGGCAGCA GGAATGAAGA TTTCCTACCA GAGCAGAGTG 
AAGGATGAGC CGGCCTACTA CTGTAACGAG TGTGATGTGG AGGTGTTCAA CCTGCTGTTT 
GTGACTAGTG AGAACGGCAG TCGGAAGATG TACGTGGTGC ACTGTGAGGA CTGTGCACGT 
CAACGCAGCC CAAACCTCTC CAATGTAGTA GTGCTGGAGC AGTACCGCAT GGAGGAGCTC 
ATGAACATGT ACGACTCCTT CAGCCTGGCG GTCAGTCCCA GCTCTCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6BA ( XM_005172240.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005172297.1 Danio rerio lysine (K)-specific demethylase 6B, a (kdm6ba), transcript variant X3, mRNA.

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CloneID	ODa12856
Clone ID Related Accession (Same CDS sequence)	XM_005172239.4 , XM_021477779.1 , XM_021477780.1 , XM_009303125.2 , XM_005172240.3 , XM_017357011.1 , XM_005172240.4 , XM_009303125.3 , XM_005172239.3
Accession Version	XM_005172240.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	lysine-specific demethylase 6B
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172240.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCACA CAGCAGAGCA GTTCACCGGG TGCAGCACAC GGGACCCTTT TCCTCTGGAC 
GGACTCAACC GGGGACCATG GGCTCCCGTG GGCAGCCGTG CCTGGCCACC TCCTTCCAGG 
TGCTTAACTG GTGTCAATCA GCACCAGCTT CTTCCCCATA TGCCCAGTCA CATGACTGGG 
CTAAATCATC CTAGTAAATT CTTGAACAAT GGACCCATAC ATCGAGGGGA TAAGCAAGAC 
CAGTCTCAGG CTTTGATGCC GGCATTGCAA AGAATGCCCC CTGCTCCTCC CAGGCCTTGG 
GATCCAACCA GAGCTGGCCA AGGGTATGAG CCATTGCCTG GTGATAACCA CAACAGATTG 
CGCAATAGCT ACAATGCAGG GCCTCCTTCA CACCTTACAG CTCGAGCCAA TCAGCTACTG 
AAGTTTGGTG CTCCTCATCC TCAATTGGCA ACCCATGGAT CACGGCCCAT TCATCCATTA 
CCTGATTTAT GGACTCCACC TGAAAATCAG CAGCAACCCA GGGGAACTCA CTCTAATTCT 
GGACAATTAA AACGTCCTGG TCCCCCTCTC GGAGAACATT CTGTTATTCA GCATACACCT 
CCTCCCTCTC TACACCGGCC CATAGAAGAC CTCCCCAGTC CGAGCAAGAG GAAGAAGAGC 
TCTGGGTCTG AACAGATTGC GCATTCAGCC ATGCAACGTT TATCTGGACC AACTGTACAT 
TTGAATCAAC AGCCATCCTC CCACTATCCC CCACCTAAAC CTGGCTTTTG GAACCCTTTG 
CACAAAGTGG GAGCACCCTG GAACCCAACT GAACACAAAA GTGGACCCAT TCATTTCCAG 
GATTCAGCTA AGCCAGAAGT TGAAAGTATC CCCTATAAAG CACCTCCTAT AAGTCTATCA 
ACCTTATCTT CTCCCACCAT TAACACCACA AGTGGCTATG GACAAAGCAG GGCCTCTTCA 
TCACAGTCAC AAAAGGCCAC TCAATCACCA CAGTCTCTGG GACCACCTTT ACAGAGCCCT 
CACATCCACC ATTCAGCATA CTCTTATTCC AACAACAGAC CTTCAGACCA GTCCCAAGGA 
GAACCACATG GTACTACTCC ACAAAGAGGA CATCATTTGG ATACCCCACC TCCGCTAATA 
CCTAAATTCT CAGCATCACT GCGGGCAGAT AGGGAGCGTC CTGCACCCTC ACCAGCAAAC 
CACACCACTG TGCCTTACAG TCACCCCCAG TTCCAGCCTC ACCCAGGGCT AGTCCATTCC 
ACCAGCCCAC CAGCCAGCAA CCAGGCCAAG GCCACAGTAC CTCAGCACAA CCTCCACAAA 
CCCTGGAGGA ACCAGGAGTC ACATGACTGT GCAGGAACAG GAGTTTCTCA GATCGCAGCA 
CGCCACTCTC AGGCACATAA TAGTGTTGTG GGTAACCAGG GTCCAAGGAC CCCCCAACAT 
GTGAGCCCAC CTCAGGCTTC TGCTCGAAAA CCTGTCATTA CTCCAAACCT AGAAACTCCT 
CATCCACCTT GCTCCCTGAA ACCATATACT AGTGTTGGAA GCATCCCTAC GATGAGCTCG 
GCACCCCTTA CAACCTCCTC AATCCACAGC ACTCTAAGCA TTGGAAGACC AACGGATTCT 
TCAGTGCTGA TGTCAAACCC AAACCATCTA TCCATGATTG GTCAAAATCC ACCACATCTT 
GGTTATCAGG GTGTTCACCC TGGTGTTGGA ACCCTTCCAC ATCTACATGG AGTGCAATCT 
CAAATTCAGC AGTCCACTCT TACCCACACA GGACAAAGTA GACCTAACTA CACCCCAGTG 
TCCTCTACTC CTTCCACCCT AAACTCAGGG CTTCAGAGAT CAGGGGACAG CGTCATCACC 
AGCAAACCCT CTAACACTCT CCCACAACCG GTCAGTTCAC CTCTAAGTTG CCCTCACCCT 
CCAGCACAAA TAAAGGCCAA CTCAATCTTG CAGCCAATGT CCAGCAGGTC ATACAACTCT 
GTAACCACTG AATCTTCCTC CTCAATTTCT CAGGCAACTA CTACTGTTCC AACTCCAGTT 
TACCCAGGAT CCAGTTTGCA ACCTACTGTC CCATGCTCAA TTGCTGATGC TTTTAATAAA 
CTTGATGCTG AGTTGCAGGG CCACATGCAA GCAGAAGAGA GAAAGAGACA ACAGGAAGAA 
AAGAGAAAAC AGATTGTAGA GAAACAAGAA GTGAACACAA AACCACATTG TGAGTCTGCC 
ATTAGAAGTC TGGAACGTTT GTTGTCAGGT AATACAACTG AGCCTCCCCC TCCTCGTTTG 
TCTCCAGCCA ATGCACCCTC TTCCATCTCG CCTTCTAGCC AGAATTCACC ACCTTATCCC 
TGGCTGAGTA GAGGAGGAGT GCCCCCTCGT CCTTCTGGAA TTTCAGCAAC CATTATAGAA 
CAATCACAAC CACCTCCTCT TACTCCCCAG ACAGAATATG CCCGTGAGAA GCAAAGGCAG 
AGGGAGAAAT TGAAGAGTGG GACACCATCT CAGAATTCTA CTGGGATTTC CACATACCAC 
TCAGAATCTA CTCTCATCAG CACCTCCAAC CACAACATGC AACTGAAACC TGACCCATCC 
AAAGACTTCA TGTTGAAAAC CGCTCAAAAT ATTGGGATAA TTGGTGCTAT TCCTAACCCT 
CCACCCCTGC GAGAGCAACC CAAACTTTAT CAAGCTTTTC ACAAGGATGC TAACCCTTCA 
TGCACATTAA TTAGCATGAC GGAAAACAAC CTTCAAAAAC AAATTCCCAG TGCAGGACTT 
CAAGGTCTGG GCAGCAATGC TAGTAGCTGC AGTGGTGATT CAGATGGTGC CCAATTTGAG 
GAAGAAACTT CTGAGCTTTT GCCTGATGGA TTAGCTAACA TTATGAAGAT GCTGGATGAG 
TCAATCAAGA AAGAGGAGGA GTTGTATTCT GGTCAGAAGG GAGAACAAGC AGTGTCTGAA 
CTTCAATTTT CTACAAATGT GGCTCCTGTG AAGAGTTATT TGTGCGCACC AGACCTTGTG 
CCTGCCCTAA AGCAATCACC AGCTGAGGAT TATCAGCCAG ATGGCCATGC AAGCCCCCCT 
GTGTTAAGTC GACAAGGTTC ACTGGCATCT CCTTGCAGCC GCACATCCTC ACTTGAGGAA 
GAAGAAGAGG TTCTTGCTTC TTCACTCAAG GTCATTCCCA AGTCTGCAAA TTCCAATAGT 
GTTCTGTCCC AGGATGGCAG TCTTGCCACA ACAGGAACCA ACTATCGCCA TAGTGACCTG 
GCCAAGCTTT ATGGCATTTC AGAGGTGGTG AAAAGTGAGT GCGAGGATGA TGAAGATGAA 
GTGGATCGAG AAGATGAAAC TCCATCCTGT TCACCACCTC AGCGCCCCCA CCTTCATCAG 
ACAGGTGTGA ACAGCATGTT TAAAAACCTT GCATCTGTAC TTGAAAGTCA GAAGTACACA 
TACCGGGGTG GTCCCTTTGG ACGTCCTCCT CGTTCTGCTT TTGTGGGAGT GAAGTACTCC 
TCATCTCTTT CTTTGGGGCC TGATATATGC AGGCAGCAAC AAAGCACTTC TCCCACATCA 
GGTTCAGCCA GTCACCCAGG CTTTAGCAGT CCAACGCAAA CCTCTGCCAT TAGCAACTCA 
GGTCAGACCT GTCCCCTCTC ACCTACTAGT TGTGAATCAG AGAGTAAAAG AGGGGATAAG 
ATAAGCCCTT CAGATCTTCT GGGCAATGAT GATGAGAAGG ATATTTCAGA AGAACCCAAA 
GTTAAGGTGG ATTCTGGAAA CATGAAAGAC TTGTTGGAAA GACGACCAAA GCTGACCACC 
ATCTCAGAGT CTTCTCTAGC AGAGCTTGGT CAAAGCTTGG AAGTGAATAA ACACATAATC 
CCTTTTAAAG ATCACTCAAA AGCAGAGGCT CGTGAGATAT GCAAACCTGT TGAAGAAAAG 
GAACGACACA GAGACAGAGA ACGAGAAAGG AAACACAAAC ACAGCAGCAG TAGCAAAAAA 
CATGAGGACA AGAAAGAGAG AAAGAAAAAG CACAGAGAAA GACATGACGA TGCATCCCTT 
TCCTCCTCCT CTTCTTCCAG CTCCAGTCGA AGACACAGGG ACGGAAAGTC ACATAAGGAA 
AAGAAGAGCA GGCAAGTGCT GGGTAATCTG GACCTTCAGA GTAAGGAGCT TAGGGAGAAA 
GAACAAGAGC GAGATGGAGA CAAGAAGAGA AGGAAAGAAG AAAGTGGCCG ACCCCAGAAT 
GAAGGGGAAG CGTGGACTCA GATTAGAGAG AAGGGCAGCA GCTCTGGACA TTCTTCCGAT 
CTCCCATCAG GTTCATCGGC ATTATGTTCG GATGACTTTC AGAAACTGAA AGCACTGACA 
GATGGGCCAC CTAAAGAACT GAAGATTCGT CTCATAAAGG TGGAGAGTGG GGACAGGGAG 
ACATTTATTG CTTCAGAAGT AGAAGAGAAG AGAATTCCTC TGAAAGATAT AACCATAAAG 
AACTCTGCTA GTGAAATTAT CAGAGCCTGC AAGGCAGCTC GTGTGAAGGG GAAATTCAAG 
GAATCCTATC TTCTGGATGC CTTTTCTGTG AAACCTGTTG TGAGGACAGA GCTGCCCATT 
CCTCGTGAGA AACTCAACCC CCCTACACCA AGCATCTATT TGGAAAGTAA AAGAGATGCA 
TTCTCTCCAG TCCTGCTGCA GTTTTGTACA GATCCTAAAA ACCCCATTAC TGTCATCCGG 
GGACTAGCTG GCTCTCTCAG ATTAAATCTT GGTTTGTTTT CCACAAAGTC TTTGGTGGAG 
GCAAATTCAG AGCATGCAGT TGAGGTCCGA ACCCAGGTCC AGCAACCAGC AGATGAGAAC 
TGGGATGCCA GCGGCACCGG ACAGACCTGG CCCTGTGAGA GCAGCAGGTC ACATACAACA 
ATTGCCAAGT ATGCCCAGTA CCAGGCATCT AGCTTCCAAG AGAGCTTACA GGAAGAGAAA 
GGCAGTGACG CTGAGGAGGA TAATGATGAA AAGCCCACCA CTAAAGCGGA AAGTACAACC 
AGTAACTCTT CGGCACCCAA CTCCAGTTCA GAGCAGAAAA CTGTGGGGAA AATAATTAAA 
TTTGGCACAA ACATTGACCT GTCTGATCCC AAAAGATGGA AGCCTCAGCT GCAGGAACTG 
CAGAAGTTGC CAGCATTTAT GCGCGTATCC TCCAGTGGGA ATATGCTAAG CCATGTTGGC 
CACACCATTT TGGGAATGAA TACAGTACAA CTATATATGA AGATTCCTGG ATGCCGCACA 
CCAGGACACC AGGAGAACAA CAACTTCTGT TCTGTTAACA TCAACATTGG ACCTGGAGAC 
TGTGAGTGGT TTGCTGTCCA TGATGACTAC TGGGAGGCCA TCAGTGATTT TTGTCAGAAA 
CATGGAGTGG ACTATTTGAC GGGTTCATGG TGGCCAGTTC TGGATGACTT GTATCGCGCT 
AACATCCCTG TGTACCGTTT CATCCAAAGG CCAGGAGATC TGGTGTGGAT CAACGCAGGC 
ACTGTACACT GGGTTCAGGC CGTGGGCTGG TGCAACAACA TTGCCTGGAA TGTGGGACCC 
GTCAACGCTT ATCAGTACCA GGTGGCTTTA GAAAGATTTG AGTGGAACGA AGTGAAGAAA 
GTGAAATCCA TCGTTCCCAT GATTCATGTG TCCTGGAATG TGGCCCGCAC TGTTAAAGTC 
ACCGACCCAG ACGCTTACAA CATGATCAAA CACTGTCTCC TCCAGTCTAT TAAGCACATT 
CAGATTCTGA GGGATCAGCT GGTGGCAGCA GGAATGAAGA TTTCCTACCA GAGCAGAGTG 
AAGGATGAGC CGGCCTACTA CTGTAACGAG TGTGATGTGG AGGTGTTCAA CCTGCTGTTT 
GTGACTAGTG AGAACGGCAG TCGGAAGATG TACGTGGTGC ACTGTGAGGA CTGTGCACGT 
CAACGCAGCC CAAACCTCTC CAATGTAGTA GTGCTGGAGC AGTACCGCAT GGAGGAGCTC 
ATGAACATGT ACGACTCCTT CAGCCTGGCG GTCAGTCCCA GCTCTCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172297.1
CDS	581..6571
Translation	MHHTAEQFTGCSTRDPFPLDGLNRGPWAPVGSRAWPPPSRCLTGVNQHQLLPHMPSHMTGLNHPSKFLNNGPIHRGDKQDQSQALMPALQRMPPAPPRPWDPTRAGQGYEPLPGDNHNRLRNSYNAGPPSHLTARANQLLKFGAPHPQLATHGSRPIHPLPDLWTPPENQQQPRGTHSNSGQLKRPGPPLGEHSVIQHTPPPSLHRPIEDLPSPSKRKKSSGSEQIAHSAMQRLSGPTVHLNQQPSSHYPPPKPGFWNPLHKVGAPWNPTEHKSGPIHFQDSAKPEVESIPYKAPPISLSTLSSPTINTTSGYGQSRASSSQSQKATQSPQSLGPPLQSPHIHHSAYSYSNNRPSDQSQGEPHGTTPQRGHHLDTPPPLIPKFSASLRADRERPAPSPANHTTVPYSHPQFQPHPGLVHSTSPPASNQAKATVPQHNLHKPWRNQESHDCAGTGVSQIAARHSQAHNSVVGNQGPRTPQHVSPPQASARKPVITPNLETPHPPCSLKPYTSVGSIPTMSSAPLTTSSIHSTLSIGRPTDSSVLMSNPNHLSMIGQNPPHLGYQGVHPGVGTLPHLHGVQSQIQQSTLTHTGQSRPNYTPVSSTPSTLNSGLQRSGDSVITSKPSNTLPQPVSSPLSCPHPPAQIKANSILQPMSSRSYNSVTTESSSSISQATTTVPTPVYPGSSLQPTVPCSIADAFNKLDAELQGHMQAEERKRQQEEKRKQIVEKQEVNTKPHCESAIRSLERLLSGNTTEPPPPRLSPANAPSSISPSSQNSPPYPWLSRGGVPPRPSGISATIIEQSQPPPLTPQTEYAREKQRQREKLKSGTPSQNSTGISTYHSESTLISTSNHNMQLKPDPSKDFMLKTAQNIGIIGAIPNPPPLREQPKLYQAFHKDANPSCTLISMTENNLQKQIPSAGLQGLGSNASSCSGDSDGAQFEEETSELLPDGLANIMKMLDESIKKEEELYSGQKGEQAVSELQFSTNVAPVKSYLCAPDLVPALKQSPAEDYQPDGHASPPVLSRQGSLASPCSRTSSLEEEEEVLASSLKVIPKSANSNSVLSQDGSLATTGTNYRHSDLAKLYGISEVVKSECEDDEDEVDREDETPSCSPPQRPHLHQTGVNSMFKNLASVLESQKYTYRGGPFGRPPRSAFVGVKYSSSLSLGPDICRQQQSTSPTSGSASHPGFSSPTQTSAISNSGQTCPLSPTSCESESKRGDKISPSDLLGNDDEKDISEEPKVKVDSGNMKDLLERRPKLTTISESSLAELGQSLEVNKHIIPFKDHSKAEAREICKPVEEKERHRDRERERKHKHSSSSKKHEDKKERKKKHRERHDDASLSSSSSSSSSRRHRDGKSHKEKKSRQVLGNLDLQSKELREKEQERDGDKKRRKEESGRPQNEGEAWTQIREKGSSSGHSSDLPSGSSALCSDDFQKLKALTDGPPKELKIRLIKVESGDRETFIASEVEEKRIPLKDITIKNSASEIIRACKAARVKGKFKESYLLDAFSVKPVVRTELPIPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPKNPITVIRGLAGSLRLNLGLFSTKSLVEANSEHAVEVRTQVQQPADENWDASGTGQTWPCESSRSHTTIAKYAQYQASSFQESLQEEKGSDAEEDNDEKPTTKAESTTSNSSAPNSSSEQKTVGKIIKFGTNIDLSDPKRWKPQLQELQKLPAFMRVSSSGNMLSHVGHTILGMNTVQLYMKIPGCRTPGHQENNNFCSVNINIGPGDCEWFAVHDDYWEAISDFCQKHGVDYLTGSWWPVLDDLYRANIPVYRFIQRPGDLVWINAGTVHWVQAVGWCNNIAWNVGPVNAYQYQVALERFEWNEVKKVKSIVPMIHVSWNVARTVKVTDPDAYNMIKHCLLQSIKHIQILRDQLVAAGMKISYQSRVKDEPAYYCNECDVEVFNLLFVTSENGSRKMYVVHCEDCARQRSPNLSNVVVLEQYRMEELMNMYDSFSLAVSPSSR

Target ORF information:

RefSeq Version	XM_005172240.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 6B, a (kdm6ba), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172240.3

ORF Insert Sequence:

ATGCATCACA CAGCAGAGCA GTTCACCGGG TGCAGCACAC GGGACCCTTT TCCTCTGGAC 
GGACTCAACC GGGGACCATG GGCTCCCGTG GGCAGCCGTG CCTGGCCACC TCCTTCCAGG 
TGCTTAACTG GTGTCAATCA GCACCAGCTT CTTCCCCATA TGCCCAGTCA CATGACTGGG 
CTAAATCATC CTAGTAAATT CTTGAACAAT GGACCCATAC ATCGAGGGGA TAAGCAAGAC 
CAGTCTCAGG CTTTGATGCC GGCATTGCAA AGAATGCCCC CTGCTCCTCC CAGGCCTTGG 
GATCCAACCA GAGCTGGCCA AGGGTATGAG CCATTGCCTG GTGATAACCA CAACAGATTG 
CGCAATAGCT ACAATGCAGG GCCTCCTTCA CACCTTACAG CTCGAGCCAA TCAGCTACTG 
AAGTTTGGTG CTCCTCATCC TCAATTGGCA ACCCATGGAT CACGGCCCAT TCATCCATTA 
CCTGATTTAT GGACTCCACC TGAAAATCAG CAGCAACCCA GGGGAACTCA CTCTAATTCT 
GGACAATTAA AACGTCCTGG TCCCCCTCTC GGAGAACATT CTGTTATTCA GCATACACCT 
CCTCCCTCTC TACACCGGCC CATAGAAGAC CTCCCCAGTC CGAGCAAGAG GAAGAAGAGC 
TCTGGGTCTG AACAGATTGC GCATTCAGCC ATGCAACGTT TATCTGGACC AACTGTACAT 
TTGAATCAAC AGCCATCCTC CCACTATCCC CCACCTAAAC CTGGCTTTTG GAACCCTTTG 
CACAAAGTGG GAGCACCCTG GAACCCAACT GAACACAAAA GTGGACCCAT TCATTTCCAG 
GATTCAGCTA AGCCAGAAGT TGAAAGTATC CCCTATAAAG CACCTCCTAT AAGTCTATCA 
ACCTTATCTT CTCCCACCAT TAACACCACA AGTGGCTATG GACAAAGCAG GGCCTCTTCA 
TCACAGTCAC AAAAGGCCAC TCAATCACCA CAGTCTCTGG GACCACCTTT ACAGAGCCCT 
CACATCCACC ATTCAGCATA CTCTTATTCC AACAACAGAC CTTCAGACCA GTCCCAAGGA 
GAACCACATG GTACTACTCC ACAAAGAGGA CATCATTTGG ATACCCCACC TCCGCTAATA 
CCTAAATTCT CAGCATCACT GCGGGCAGAT AGGGAGCGTC CTGCACCCTC ACCAGCAAAC 
CACACCACTG TGCCTTACAG TCACCCCCAG TTCCAGCCTC ACCCAGGGCT AGTCCATTCC 
ACCAGCCCAC CAGCCAGCAA CCAGGCCAAG GCCACAGTAC CTCAGCACAA CCTCCACAAA 
CCCTGGAGGA ACCAGGAGTC ACATGACTGT GCAGGAACAG GAGTTTCTCA GATCGCAGCA 
CGCCACTCTC AGGCACATAA TAGTGTTGTG GGTAACCAGG GTCCAAGGAC CCCCCAACAT 
GTGAGCCCAC CTCAGGCTTC TGCTCGAAAA CCTGTCATTA CTCCAAACCT AGAAACTCCT 
CATCCACCTT GCTCCCTGAA ACCATATACT AGTGTTGGAA GCATCCCTAC GATGAGCTCG 
GCACCCCTTA CAACCTCCTC AATCCACAGC ACTCTAAGCA TTGGAAGACC AACGGATTCT 
TCAGTGCTGA TGTCAAACCC AAACCATCTA TCCATGATTG GTCAAAATCC ACCACATCTT 
GGTTATCAGG GTGTTCACCC TGGTGTTGGA ACCCTTCCAC ATCTACATGG AGTGCAATCT 
CAAATTCAGC AGTCCACTCT TACCCACACA GGACAAAGTA GACCTAACTA CACCCCAGTG 
TCCTCTACTC CTTCCACCCT AAACTCAGGG CTTCAGAGAT CAGGGGACAG CGTCATCACC 
AGCAAACCCT CTAACACTCT CCCACAACCG GTCAGTTCAC CTCTAAGTTG CCCTCACCCT 
CCAGCACAAA TAAAGGCCAA CTCAATCTTG CAGCCAATGT CCAGCAGGTC ATACAACTCT 
GTAACCACTG AATCTTCCTC CTCAATTTCT CAGGCAACTA CTACTGTTCC AACTCCAGTT 
TACCCAGGAT CCAGTTTGCA ACCTACTGTC CCATGCTCAA TTGCTGATGC TTTTAATAAA 
CTTGATGCTG AGTTGCAGGG CCACATGCAA GCAGAAGAGA GAAAGAGACA ACAGGAAGAA 
AAGAGAAAAC AGATTGTAGA GAAACAAGAA GTGAACACAA AACCACATTG TGAGTCTGCC 
ATTAGAAGTC TGGAACGTTT GTTGTCAGGT AATACAACTG AGCCTCCCCC TCCTCGTTTG 
TCTCCAGCCA ATGCACCCTC TTCCATCTCG CCTTCTAGCC AGAATTCACC ACCTTATCCC 
TGGCTGAGTA GAGGAGGAGT GCCCCCTCGT CCTTCTGGAA TTTCAGCAAC CATTATAGAA 
CAATCACAAC CACCTCCTCT TACTCCCCAG ACAGAATATG CCCGTGAGAA GCAAAGGCAG 
AGGGAGAAAT TGAAGAGTGG GACACCATCT CAGAATTCTA CTGGGATTTC CACATACCAC 
TCAGAATCTA CTCTCATCAG CACCTCCAAC CACAACATGC AACTGAAACC TGACCCATCC 
AAAGACTTCA TGTTGAAAAC CGCTCAAAAT ATTGGGATAA TTGGTGCTAT TCCTAACCCT 
CCACCCCTGC GAGAGCAACC CAAACTTTAT CAAGCTTTTC ACAAGGATGC TAACCCTTCA 
TGCACATTAA TTAGCATGAC GGAAAACAAC CTTCAAAAAC AAATTCCCAG TGCAGGACTT 
CAAGGTCTGG GCAGCAATGC TAGTAGCTGC AGTGGTGATT CAGATGGTGC CCAATTTGAG 
GAAGAAACTT CTGAGCTTTT GCCTGATGGA TTAGCTAACA TTATGAAGAT GCTGGATGAG 
TCAATCAAGA AAGAGGAGGA GTTGTATTCT GGTCAGAAGG GAGAACAAGC AGTGTCTGAA 
CTTCAATTTT CTACAAATGT GGCTCCTGTG AAGAGTTATT TGTGCGCACC AGACCTTGTG 
CCTGCCCTAA AGCAATCACC AGCTGAGGAT TATCAGCCAG ATGGCCATGC AAGCCCCCCT 
GTGTTAAGTC GACAAGGTTC ACTGGCATCT CCTTGCAGCC GCACATCCTC ACTTGAGGAA 
GAAGAAGAGG TTCTTGCTTC TTCACTCAAG GTCATTCCCA AGTCTGCAAA TTCCAATAGT 
GTTCTGTCCC AGGATGGCAG TCTTGCCACA ACAGGAACCA ACTATCGCCA TAGTGACCTG 
GCCAAGCTTT ATGGCATTTC AGAGGTGGTG AAAAGTGAGT GCGAGGATGA TGAAGATGAA 
GTGGATCGAG AAGATGAAAC TCCATCCTGT TCACCACCTC AGCGCCCCCA CCTTCATCAG 
ACAGGTGTGA ACAGCATGTT TAAAAACCTT GCATCTGTAC TTGAAAGTCA GAAGTACACA 
TACCGGGGTG GTCCCTTTGG ACGTCCTCCT CGTTCTGCTT TTGTGGGAGT GAAGTACTCC 
TCATCTCTTT CTTTGGGGCC TGATATATGC AGGCAGCAAC AAAGCACTTC TCCCACATCA 
GGTTCAGCCA GTCACCCAGG CTTTAGCAGT CCAACGCAAA CCTCTGCCAT TAGCAACTCA 
GGTCAGACCT GTCCCCTCTC ACCTACTAGT TGTGAATCAG AGAGTAAAAG AGGGGATAAG 
ATAAGCCCTT CAGATCTTCT GGGCAATGAT GATGAGAAGG ATATTTCAGA AGAACCCAAA 
GTTAAGGTGG ATTCTGGAAA CATGAAAGAC TTGTTGGAAA GACGACCAAA GCTGACCACC 
ATCTCAGAGT CTTCTCTAGC AGAGCTTGGT CAAAGCTTGG AAGTGAATAA ACACATAATC 
CCTTTTAAAG ATCACTCAAA AGCAGAGGCT CGTGAGATAT GCAAACCTGT TGAAGAAAAG 
GAACGACACA GAGACAGAGA ACGAGAAAGG AAACACAAAC ACAGCAGCAG TAGCAAAAAA 
CATGAGGACA AGAAAGAGAG AAAGAAAAAG CACAGAGAAA GACATGACGA TGCATCCCTT 
TCCTCCTCCT CTTCTTCCAG CTCCAGTCGA AGACACAGGG ACGGAAAGTC ACATAAGGAA 
AAGAAGAGCA GGCAAGTGCT GGGTAATCTG GACCTTCAGA GTAAGGAGCT TAGGGAGAAA 
GAACAAGAGC GAGATGGAGA CAAGAAGAGA AGGAAAGAAG AAAGTGGCCG ACCCCAGAAT 
GAAGGGGAAG CGTGGACTCA GATTAGAGAG AAGGGCAGCA GCTCTGGACA TTCTTCCGAT 
CTCCCATCAG GTTCATCGGC ATTATGTTCG GATGACTTTC AGAAACTGAA AGCACTGACA 
GATGGGCCAC CTAAAGAACT GAAGATTCGT CTCATAAAGG TGGAGAGTGG GGACAGGGAG 
ACATTTATTG CTTCAGAAGT AGAAGAGAAG AGAATTCCTC TGAAAGATAT AACCATAAAG 
AACTCTGCTA GTGAAATTAT CAGAGCCTGC AAGGCAGCTC GTGTGAAGGG GAAATTCAAG 
GAATCCTATC TTCTGGATGC CTTTTCTGTG AAACCTGTTG TGAGGACAGA GCTGCCCATT 
CCTCGTGAGA AACTCAACCC CCCTACACCA AGCATCTATT TGGAAAGTAA AAGAGATGCA 
TTCTCTCCAG TCCTGCTGCA GTTTTGTACA GATCCTAAAA ACCCCATTAC TGTCATCCGG 
GGACTAGCTG GCTCTCTCAG ATTAAATCTT GGTTTGTTTT CCACAAAGTC TTTGGTGGAG 
GCAAATTCAG AGCATGCAGT TGAGGTCCGA ACCCAGGTCC AGCAACCAGC AGATGAGAAC 
TGGGATGCCA GCGGCACCGG ACAGACCTGG CCCTGTGAGA GCAGCAGGTC ACATACAACA 
ATTGCCAAGT ATGCCCAGTA CCAGGCATCT AGCTTCCAAG AGAGCTTACA GGAAGAGAAA 
GGCAGTGACG CTGAGGAGGA TAATGATGAA AAGCCCACCA CTAAAGCGGA AAGTACAACC 
AGTAACTCTT CGGCACCCAA CTCCAGTTCA GAGCAGAAAA CTGTGGGGAA AATAATTAAA 
TTTGGCACAA ACATTGACCT GTCTGATCCC AAAAGATGGA AGCCTCAGCT GCAGGAACTG 
CAGAAGTTGC CAGCATTTAT GCGCGTATCC TCCAGTGGGA ATATGCTAAG CCATGTTGGC 
CACACCATTT TGGGAATGAA TACAGTACAA CTATATATGA AGATTCCTGG ATGCCGCACA 
CCAGGACACC AGGAGAACAA CAACTTCTGT TCTGTTAACA TCAACATTGG ACCTGGAGAC 
TGTGAGTGGT TTGCTGTCCA TGATGACTAC TGGGAGGCCA TCAGTGATTT TTGTCAGAAA 
CATGGAGTGG ACTATTTGAC GGGTTCATGG TGGCCAGTTC TGGATGACTT GTATCGCGCT 
AACATCCCTG TGTACCGTTT CATCCAAAGG CCAGGAGATC TGGTGTGGAT CAACGCAGGC 
ACTGTACACT GGGTTCAGGC CGTGGGCTGG TGCAACAACA TTGCCTGGAA TGTGGGACCC 
GTCAACGCTT ATCAGTACCA GGTGGCTTTA GAAAGATTTG AGTGGAACGA AGTGAAGAAA 
GTGAAATCCA TCGTTCCCAT GATTCATGTG TCCTGGAATG TGGCCCGCAC TGTTAAAGTC 
ACCGACCCAG ACGCTTACAA CATGATCAAA CACTGTCTCC TCCAGTCTAT TAAGCACATT 
CAGATTCTGA GGGATCAGCT GGTGGCAGCA GGAATGAAGA TTTCCTACCA GAGCAGAGTG 
AAGGATGAGC CGGCCTACTA CTGTAACGAG TGTGATGTGG AGGTGTTCAA CCTGCTGTTT 
GTGACTAGTG AGAACGGCAG TCGGAAGATG TACGTGGTGC ACTGTGAGGA CTGTGCACGT 
CAACGCAGCC CAAACCTCTC CAATGTAGTA GTGCTGGAGC AGTACCGCAT GGAGGAGCTC 
ATGAACATGT ACGACTCCTT CAGCCTGGCG GTCAGTCCCA GCTCTCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6BA ( XM_017357011.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017212500.1 Danio rerio lysine (K)-specific demethylase 6B, a (kdm6ba), transcript variant X4, mRNA.

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CloneID	ODa12856
Clone ID Related Accession (Same CDS sequence)	XM_005172239.4 , XM_021477779.1 , XM_021477780.1 , XM_009303125.2 , XM_005172240.3 , XM_017357011.1 , XM_005172240.4 , XM_009303125.3 , XM_005172239.3
Accession Version	XM_017357011.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	lysine-specific demethylase 6B
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCACA CAGCAGAGCA GTTCACCGGG TGCAGCACAC GGGACCCTTT TCCTCTGGAC 
GGACTCAACC GGGGACCATG GGCTCCCGTG GGCAGCCGTG CCTGGCCACC TCCTTCCAGG 
TGCTTAACTG GTGTCAATCA GCACCAGCTT CTTCCCCATA TGCCCAGTCA CATGACTGGG 
CTAAATCATC CTAGTAAATT CTTGAACAAT GGACCCATAC ATCGAGGGGA TAAGCAAGAC 
CAGTCTCAGG CTTTGATGCC GGCATTGCAA AGAATGCCCC CTGCTCCTCC CAGGCCTTGG 
GATCCAACCA GAGCTGGCCA AGGGTATGAG CCATTGCCTG GTGATAACCA CAACAGATTG 
CGCAATAGCT ACAATGCAGG GCCTCCTTCA CACCTTACAG CTCGAGCCAA TCAGCTACTG 
AAGTTTGGTG CTCCTCATCC TCAATTGGCA ACCCATGGAT CACGGCCCAT TCATCCATTA 
CCTGATTTAT GGACTCCACC TGAAAATCAG CAGCAACCCA GGGGAACTCA CTCTAATTCT 
GGACAATTAA AACGTCCTGG TCCCCCTCTC GGAGAACATT CTGTTATTCA GCATACACCT 
CCTCCCTCTC TACACCGGCC CATAGAAGAC CTCCCCAGTC CGAGCAAGAG GAAGAAGAGC 
TCTGGGTCTG AACAGATTGC GCATTCAGCC ATGCAACGTT TATCTGGACC AACTGTACAT 
TTGAATCAAC AGCCATCCTC CCACTATCCC CCACCTAAAC CTGGCTTTTG GAACCCTTTG 
CACAAAGTGG GAGCACCCTG GAACCCAACT GAACACAAAA GTGGACCCAT TCATTTCCAG 
GATTCAGCTA AGCCAGAAGT TGAAAGTATC CCCTATAAAG CACCTCCTAT AAGTCTATCA 
ACCTTATCTT CTCCCACCAT TAACACCACA AGTGGCTATG GACAAAGCAG GGCCTCTTCA 
TCACAGTCAC AAAAGGCCAC TCAATCACCA CAGTCTCTGG GACCACCTTT ACAGAGCCCT 
CACATCCACC ATTCAGCATA CTCTTATTCC AACAACAGAC CTTCAGACCA GTCCCAAGGA 
GAACCACATG GTACTACTCC ACAAAGAGGA CATCATTTGG ATACCCCACC TCCGCTAATA 
CCTAAATTCT CAGCATCACT GCGGGCAGAT AGGGAGCGTC CTGCACCCTC ACCAGCAAAC 
CACACCACTG TGCCTTACAG TCACCCCCAG TTCCAGCCTC ACCCAGGGCT AGTCCATTCC 
ACCAGCCCAC CAGCCAGCAA CCAGGCCAAG GCCACAGTAC CTCAGCACAA CCTCCACAAA 
CCCTGGAGGA ACCAGGAGTC ACATGACTGT GCAGGAACAG GAGTTTCTCA GATCGCAGCA 
CGCCACTCTC AGGCACATAA TAGTGTTGTG GGTAACCAGG GTCCAAGGAC CCCCCAACAT 
GTGAGCCCAC CTCAGGCTTC TGCTCGAAAA CCTGTCATTA CTCCAAACCT AGAAACTCCT 
CATCCACCTT GCTCCCTGAA ACCATATACT AGTGTTGGAA GCATCCCTAC GATGAGCTCG 
GCACCCCTTA CAACCTCCTC AATCCACAGC ACTCTAAGCA TTGGAAGACC AACGGATTCT 
TCAGTGCTGA TGTCAAACCC AAACCATCTA TCCATGATTG GTCAAAATCC ACCACATCTT 
GGTTATCAGG GTGTTCACCC TGGTGTTGGA ACCCTTCCAC ATCTACATGG AGTGCAATCT 
CAAATTCAGC AGTCCACTCT TACCCACACA GGACAAAGTA GACCTAACTA CACCCCAGTG 
TCCTCTACTC CTTCCACCCT AAACTCAGGG CTTCAGAGAT CAGGGGACAG CGTCATCACC 
AGCAAACCCT CTAACACTCT CCCACAACCG GTCAGTTCAC CTCTAAGTTG CCCTCACCCT 
CCAGCACAAA TAAAGGCCAA CTCAATCTTG CAGCCAATGT CCAGCAGGTC ATACAACTCT 
GTAACCACTG AATCTTCCTC CTCAATTTCT CAGGCAACTA CTACTGTTCC AACTCCAGTT 
TACCCAGGAT CCAGTTTGCA ACCTACTGTC CCATGCTCAA TTGCTGATGC TTTTAATAAA 
CTTGATGCTG AGTTGCAGGG CCACATGCAA GCAGAAGAGA GAAAGAGACA ACAGGAAGAA 
AAGAGAAAAC AGATTGTAGA GAAACAAGAA GTGAACACAA AACCACATTG TGAGTCTGCC 
ATTAGAAGTC TGGAACGTTT GTTGTCAGGT AATACAACTG AGCCTCCCCC TCCTCGTTTG 
TCTCCAGCCA ATGCACCCTC TTCCATCTCG CCTTCTAGCC AGAATTCACC ACCTTATCCC 
TGGCTGAGTA GAGGAGGAGT GCCCCCTCGT CCTTCTGGAA TTTCAGCAAC CATTATAGAA 
CAATCACAAC CACCTCCTCT TACTCCCCAG ACAGAATATG CCCGTGAGAA GCAAAGGCAG 
AGGGAGAAAT TGAAGAGTGG GACACCATCT CAGAATTCTA CTGGGATTTC CACATACCAC 
TCAGAATCTA CTCTCATCAG CACCTCCAAC CACAACATGC AACTGAAACC TGACCCATCC 
AAAGACTTCA TGTTGAAAAC CGCTCAAAAT ATTGGGATAA TTGGTGCTAT TCCTAACCCT 
CCACCCCTGC GAGAGCAACC CAAACTTTAT CAAGCTTTTC ACAAGGATGC TAACCCTTCA 
TGCACATTAA TTAGCATGAC GGAAAACAAC CTTCAAAAAC AAATTCCCAG TGCAGGACTT 
CAAGGTCTGG GCAGCAATGC TAGTAGCTGC AGTGGTGATT CAGATGGTGC CCAATTTGAG 
GAAGAAACTT CTGAGCTTTT GCCTGATGGA TTAGCTAACA TTATGAAGAT GCTGGATGAG 
TCAATCAAGA AAGAGGAGGA GTTGTATTCT GGTCAGAAGG GAGAACAAGC AGTGTCTGAA 
CTTCAATTTT CTACAAATGT GGCTCCTGTG AAGAGTTATT TGTGCGCACC AGACCTTGTG 
CCTGCCCTAA AGCAATCACC AGCTGAGGAT TATCAGCCAG ATGGCCATGC AAGCCCCCCT 
GTGTTAAGTC GACAAGGTTC ACTGGCATCT CCTTGCAGCC GCACATCCTC ACTTGAGGAA 
GAAGAAGAGG TTCTTGCTTC TTCACTCAAG GTCATTCCCA AGTCTGCAAA TTCCAATAGT 
GTTCTGTCCC AGGATGGCAG TCTTGCCACA ACAGGAACCA ACTATCGCCA TAGTGACCTG 
GCCAAGCTTT ATGGCATTTC AGAGGTGGTG AAAAGTGAGT GCGAGGATGA TGAAGATGAA 
GTGGATCGAG AAGATGAAAC TCCATCCTGT TCACCACCTC AGCGCCCCCA CCTTCATCAG 
ACAGGTGTGA ACAGCATGTT TAAAAACCTT GCATCTGTAC TTGAAAGTCA GAAGTACACA 
TACCGGGGTG GTCCCTTTGG ACGTCCTCCT CGTTCTGCTT TTGTGGGAGT GAAGTACTCC 
TCATCTCTTT CTTTGGGGCC TGATATATGC AGGCAGCAAC AAAGCACTTC TCCCACATCA 
GGTTCAGCCA GTCACCCAGG CTTTAGCAGT CCAACGCAAA CCTCTGCCAT TAGCAACTCA 
GGTCAGACCT GTCCCCTCTC ACCTACTAGT TGTGAATCAG AGAGTAAAAG AGGGGATAAG 
ATAAGCCCTT CAGATCTTCT GGGCAATGAT GATGAGAAGG ATATTTCAGA AGAACCCAAA 
GTTAAGGTGG ATTCTGGAAA CATGAAAGAC TTGTTGGAAA GACGACCAAA GCTGACCACC 
ATCTCAGAGT CTTCTCTAGC AGAGCTTGGT CAAAGCTTGG AAGTGAATAA ACACATAATC 
CCTTTTAAAG ATCACTCAAA AGCAGAGGCT CGTGAGATAT GCAAACCTGT TGAAGAAAAG 
GAACGACACA GAGACAGAGA ACGAGAAAGG AAACACAAAC ACAGCAGCAG TAGCAAAAAA 
CATGAGGACA AGAAAGAGAG AAAGAAAAAG CACAGAGAAA GACATGACGA TGCATCCCTT 
TCCTCCTCCT CTTCTTCCAG CTCCAGTCGA AGACACAGGG ACGGAAAGTC ACATAAGGAA 
AAGAAGAGCA GGCAAGTGCT GGGTAATCTG GACCTTCAGA GTAAGGAGCT TAGGGAGAAA 
GAACAAGAGC GAGATGGAGA CAAGAAGAGA AGGAAAGAAG AAAGTGGCCG ACCCCAGAAT 
GAAGGGGAAG CGTGGACTCA GATTAGAGAG AAGGGCAGCA GCTCTGGACA TTCTTCCGAT 
CTCCCATCAG GTTCATCGGC ATTATGTTCG GATGACTTTC AGAAACTGAA AGCACTGACA 
GATGGGCCAC CTAAAGAACT GAAGATTCGT CTCATAAAGG TGGAGAGTGG GGACAGGGAG 
ACATTTATTG CTTCAGAAGT AGAAGAGAAG AGAATTCCTC TGAAAGATAT AACCATAAAG 
AACTCTGCTA GTGAAATTAT CAGAGCCTGC AAGGCAGCTC GTGTGAAGGG GAAATTCAAG 
GAATCCTATC TTCTGGATGC CTTTTCTGTG AAACCTGTTG TGAGGACAGA GCTGCCCATT 
CCTCGTGAGA AACTCAACCC CCCTACACCA AGCATCTATT TGGAAAGTAA AAGAGATGCA 
TTCTCTCCAG TCCTGCTGCA GTTTTGTACA GATCCTAAAA ACCCCATTAC TGTCATCCGG 
GGACTAGCTG GCTCTCTCAG ATTAAATCTT GGTTTGTTTT CCACAAAGTC TTTGGTGGAG 
GCAAATTCAG AGCATGCAGT TGAGGTCCGA ACCCAGGTCC AGCAACCAGC AGATGAGAAC 
TGGGATGCCA GCGGCACCGG ACAGACCTGG CCCTGTGAGA GCAGCAGGTC ACATACAACA 
ATTGCCAAGT ATGCCCAGTA CCAGGCATCT AGCTTCCAAG AGAGCTTACA GGAAGAGAAA 
GGCAGTGACG CTGAGGAGGA TAATGATGAA AAGCCCACCA CTAAAGCGGA AAGTACAACC 
AGTAACTCTT CGGCACCCAA CTCCAGTTCA GAGCAGAAAA CTGTGGGGAA AATAATTAAA 
TTTGGCACAA ACATTGACCT GTCTGATCCC AAAAGATGGA AGCCTCAGCT GCAGGAACTG 
CAGAAGTTGC CAGCATTTAT GCGCGTATCC TCCAGTGGGA ATATGCTAAG CCATGTTGGC 
CACACCATTT TGGGAATGAA TACAGTACAA CTATATATGA AGATTCCTGG ATGCCGCACA 
CCAGGACACC AGGAGAACAA CAACTTCTGT TCTGTTAACA TCAACATTGG ACCTGGAGAC 
TGTGAGTGGT TTGCTGTCCA TGATGACTAC TGGGAGGCCA TCAGTGATTT TTGTCAGAAA 
CATGGAGTGG ACTATTTGAC GGGTTCATGG TGGCCAGTTC TGGATGACTT GTATCGCGCT 
AACATCCCTG TGTACCGTTT CATCCAAAGG CCAGGAGATC TGGTGTGGAT CAACGCAGGC 
ACTGTACACT GGGTTCAGGC CGTGGGCTGG TGCAACAACA TTGCCTGGAA TGTGGGACCC 
GTCAACGCTT ATCAGTACCA GGTGGCTTTA GAAAGATTTG AGTGGAACGA AGTGAAGAAA 
GTGAAATCCA TCGTTCCCAT GATTCATGTG TCCTGGAATG TGGCCCGCAC TGTTAAAGTC 
ACCGACCCAG ACGCTTACAA CATGATCAAA CACTGTCTCC TCCAGTCTAT TAAGCACATT 
CAGATTCTGA GGGATCAGCT GGTGGCAGCA GGAATGAAGA TTTCCTACCA GAGCAGAGTG 
AAGGATGAGC CGGCCTACTA CTGTAACGAG TGTGATGTGG AGGTGTTCAA CCTGCTGTTT 
GTGACTAGTG AGAACGGCAG TCGGAAGATG TACGTGGTGC ACTGTGAGGA CTGTGCACGT 
CAACGCAGCC CAAACCTCTC CAATGTAGTA GTGCTGGAGC AGTACCGCAT GGAGGAGCTC 
ATGAACATGT ACGACTCCTT CAGCCTGGCG GTCAGTCCCA GCTCTCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212500.1
CDS	963..6953
Translation	MHHTAEQFTGCSTRDPFPLDGLNRGPWAPVGSRAWPPPSRCLTGVNQHQLLPHMPSHMTGLNHPSKFLNNGPIHRGDKQDQSQALMPALQRMPPAPPRPWDPTRAGQGYEPLPGDNHNRLRNSYNAGPPSHLTARANQLLKFGAPHPQLATHGSRPIHPLPDLWTPPENQQQPRGTHSNSGQLKRPGPPLGEHSVIQHTPPPSLHRPIEDLPSPSKRKKSSGSEQIAHSAMQRLSGPTVHLNQQPSSHYPPPKPGFWNPLHKVGAPWNPTEHKSGPIHFQDSAKPEVESIPYKAPPISLSTLSSPTINTTSGYGQSRASSSQSQKATQSPQSLGPPLQSPHIHHSAYSYSNNRPSDQSQGEPHGTTPQRGHHLDTPPPLIPKFSASLRADRERPAPSPANHTTVPYSHPQFQPHPGLVHSTSPPASNQAKATVPQHNLHKPWRNQESHDCAGTGVSQIAARHSQAHNSVVGNQGPRTPQHVSPPQASARKPVITPNLETPHPPCSLKPYTSVGSIPTMSSAPLTTSSIHSTLSIGRPTDSSVLMSNPNHLSMIGQNPPHLGYQGVHPGVGTLPHLHGVQSQIQQSTLTHTGQSRPNYTPVSSTPSTLNSGLQRSGDSVITSKPSNTLPQPVSSPLSCPHPPAQIKANSILQPMSSRSYNSVTTESSSSISQATTTVPTPVYPGSSLQPTVPCSIADAFNKLDAELQGHMQAEERKRQQEEKRKQIVEKQEVNTKPHCESAIRSLERLLSGNTTEPPPPRLSPANAPSSISPSSQNSPPYPWLSRGGVPPRPSGISATIIEQSQPPPLTPQTEYAREKQRQREKLKSGTPSQNSTGISTYHSESTLISTSNHNMQLKPDPSKDFMLKTAQNIGIIGAIPNPPPLREQPKLYQAFHKDANPSCTLISMTENNLQKQIPSAGLQGLGSNASSCSGDSDGAQFEEETSELLPDGLANIMKMLDESIKKEEELYSGQKGEQAVSELQFSTNVAPVKSYLCAPDLVPALKQSPAEDYQPDGHASPPVLSRQGSLASPCSRTSSLEEEEEVLASSLKVIPKSANSNSVLSQDGSLATTGTNYRHSDLAKLYGISEVVKSECEDDEDEVDREDETPSCSPPQRPHLHQTGVNSMFKNLASVLESQKYTYRGGPFGRPPRSAFVGVKYSSSLSLGPDICRQQQSTSPTSGSASHPGFSSPTQTSAISNSGQTCPLSPTSCESESKRGDKISPSDLLGNDDEKDISEEPKVKVDSGNMKDLLERRPKLTTISESSLAELGQSLEVNKHIIPFKDHSKAEAREICKPVEEKERHRDRERERKHKHSSSSKKHEDKKERKKKHRERHDDASLSSSSSSSSSRRHRDGKSHKEKKSRQVLGNLDLQSKELREKEQERDGDKKRRKEESGRPQNEGEAWTQIREKGSSSGHSSDLPSGSSALCSDDFQKLKALTDGPPKELKIRLIKVESGDRETFIASEVEEKRIPLKDITIKNSASEIIRACKAARVKGKFKESYLLDAFSVKPVVRTELPIPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPKNPITVIRGLAGSLRLNLGLFSTKSLVEANSEHAVEVRTQVQQPADENWDASGTGQTWPCESSRSHTTIAKYAQYQASSFQESLQEEKGSDAEEDNDEKPTTKAESTTSNSSAPNSSSEQKTVGKIIKFGTNIDLSDPKRWKPQLQELQKLPAFMRVSSSGNMLSHVGHTILGMNTVQLYMKIPGCRTPGHQENNNFCSVNINIGPGDCEWFAVHDDYWEAISDFCQKHGVDYLTGSWWPVLDDLYRANIPVYRFIQRPGDLVWINAGTVHWVQAVGWCNNIAWNVGPVNAYQYQVALERFEWNEVKKVKSIVPMIHVSWNVARTVKVTDPDAYNMIKHCLLQSIKHIQILRDQLVAAGMKISYQSRVKDEPAYYCNECDVEVFNLLFVTSENGSRKMYVVHCEDCARQRSPNLSNVVVLEQYRMEELMNMYDSFSLAVSPSSR

Target ORF information:

RefSeq Version	XM_017357011.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 6B, a (kdm6ba), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357011.1

ORF Insert Sequence:

ATGCATCACA CAGCAGAGCA GTTCACCGGG TGCAGCACAC GGGACCCTTT TCCTCTGGAC 
GGACTCAACC GGGGACCATG GGCTCCCGTG GGCAGCCGTG CCTGGCCACC TCCTTCCAGG 
TGCTTAACTG GTGTCAATCA GCACCAGCTT CTTCCCCATA TGCCCAGTCA CATGACTGGG 
CTAAATCATC CTAGTAAATT CTTGAACAAT GGACCCATAC ATCGAGGGGA TAAGCAAGAC 
CAGTCTCAGG CTTTGATGCC GGCATTGCAA AGAATGCCCC CTGCTCCTCC CAGGCCTTGG 
GATCCAACCA GAGCTGGCCA AGGGTATGAG CCATTGCCTG GTGATAACCA CAACAGATTG 
CGCAATAGCT ACAATGCAGG GCCTCCTTCA CACCTTACAG CTCGAGCCAA TCAGCTACTG 
AAGTTTGGTG CTCCTCATCC TCAATTGGCA ACCCATGGAT CACGGCCCAT TCATCCATTA 
CCTGATTTAT GGACTCCACC TGAAAATCAG CAGCAACCCA GGGGAACTCA CTCTAATTCT 
GGACAATTAA AACGTCCTGG TCCCCCTCTC GGAGAACATT CTGTTATTCA GCATACACCT 
CCTCCCTCTC TACACCGGCC CATAGAAGAC CTCCCCAGTC CGAGCAAGAG GAAGAAGAGC 
TCTGGGTCTG AACAGATTGC GCATTCAGCC ATGCAACGTT TATCTGGACC AACTGTACAT 
TTGAATCAAC AGCCATCCTC CCACTATCCC CCACCTAAAC CTGGCTTTTG GAACCCTTTG 
CACAAAGTGG GAGCACCCTG GAACCCAACT GAACACAAAA GTGGACCCAT TCATTTCCAG 
GATTCAGCTA AGCCAGAAGT TGAAAGTATC CCCTATAAAG CACCTCCTAT AAGTCTATCA 
ACCTTATCTT CTCCCACCAT TAACACCACA AGTGGCTATG GACAAAGCAG GGCCTCTTCA 
TCACAGTCAC AAAAGGCCAC TCAATCACCA CAGTCTCTGG GACCACCTTT ACAGAGCCCT 
CACATCCACC ATTCAGCATA CTCTTATTCC AACAACAGAC CTTCAGACCA GTCCCAAGGA 
GAACCACATG GTACTACTCC ACAAAGAGGA CATCATTTGG ATACCCCACC TCCGCTAATA 
CCTAAATTCT CAGCATCACT GCGGGCAGAT AGGGAGCGTC CTGCACCCTC ACCAGCAAAC 
CACACCACTG TGCCTTACAG TCACCCCCAG TTCCAGCCTC ACCCAGGGCT AGTCCATTCC 
ACCAGCCCAC CAGCCAGCAA CCAGGCCAAG GCCACAGTAC CTCAGCACAA CCTCCACAAA 
CCCTGGAGGA ACCAGGAGTC ACATGACTGT GCAGGAACAG GAGTTTCTCA GATCGCAGCA 
CGCCACTCTC AGGCACATAA TAGTGTTGTG GGTAACCAGG GTCCAAGGAC CCCCCAACAT 
GTGAGCCCAC CTCAGGCTTC TGCTCGAAAA CCTGTCATTA CTCCAAACCT AGAAACTCCT 
CATCCACCTT GCTCCCTGAA ACCATATACT AGTGTTGGAA GCATCCCTAC GATGAGCTCG 
GCACCCCTTA CAACCTCCTC AATCCACAGC ACTCTAAGCA TTGGAAGACC AACGGATTCT 
TCAGTGCTGA TGTCAAACCC AAACCATCTA TCCATGATTG GTCAAAATCC ACCACATCTT 
GGTTATCAGG GTGTTCACCC TGGTGTTGGA ACCCTTCCAC ATCTACATGG AGTGCAATCT 
CAAATTCAGC AGTCCACTCT TACCCACACA GGACAAAGTA GACCTAACTA CACCCCAGTG 
TCCTCTACTC CTTCCACCCT AAACTCAGGG CTTCAGAGAT CAGGGGACAG CGTCATCACC 
AGCAAACCCT CTAACACTCT CCCACAACCG GTCAGTTCAC CTCTAAGTTG CCCTCACCCT 
CCAGCACAAA TAAAGGCCAA CTCAATCTTG CAGCCAATGT CCAGCAGGTC ATACAACTCT 
GTAACCACTG AATCTTCCTC CTCAATTTCT CAGGCAACTA CTACTGTTCC AACTCCAGTT 
TACCCAGGAT CCAGTTTGCA ACCTACTGTC CCATGCTCAA TTGCTGATGC TTTTAATAAA 
CTTGATGCTG AGTTGCAGGG CCACATGCAA GCAGAAGAGA GAAAGAGACA ACAGGAAGAA 
AAGAGAAAAC AGATTGTAGA GAAACAAGAA GTGAACACAA AACCACATTG TGAGTCTGCC 
ATTAGAAGTC TGGAACGTTT GTTGTCAGGT AATACAACTG AGCCTCCCCC TCCTCGTTTG 
TCTCCAGCCA ATGCACCCTC TTCCATCTCG CCTTCTAGCC AGAATTCACC ACCTTATCCC 
TGGCTGAGTA GAGGAGGAGT GCCCCCTCGT CCTTCTGGAA TTTCAGCAAC CATTATAGAA 
CAATCACAAC CACCTCCTCT TACTCCCCAG ACAGAATATG CCCGTGAGAA GCAAAGGCAG 
AGGGAGAAAT TGAAGAGTGG GACACCATCT CAGAATTCTA CTGGGATTTC CACATACCAC 
TCAGAATCTA CTCTCATCAG CACCTCCAAC CACAACATGC AACTGAAACC TGACCCATCC 
AAAGACTTCA TGTTGAAAAC CGCTCAAAAT ATTGGGATAA TTGGTGCTAT TCCTAACCCT 
CCACCCCTGC GAGAGCAACC CAAACTTTAT CAAGCTTTTC ACAAGGATGC TAACCCTTCA 
TGCACATTAA TTAGCATGAC GGAAAACAAC CTTCAAAAAC AAATTCCCAG TGCAGGACTT 
CAAGGTCTGG GCAGCAATGC TAGTAGCTGC AGTGGTGATT CAGATGGTGC CCAATTTGAG 
GAAGAAACTT CTGAGCTTTT GCCTGATGGA TTAGCTAACA TTATGAAGAT GCTGGATGAG 
TCAATCAAGA AAGAGGAGGA GTTGTATTCT GGTCAGAAGG GAGAACAAGC AGTGTCTGAA 
CTTCAATTTT CTACAAATGT GGCTCCTGTG AAGAGTTATT TGTGCGCACC AGACCTTGTG 
CCTGCCCTAA AGCAATCACC AGCTGAGGAT TATCAGCCAG ATGGCCATGC AAGCCCCCCT 
GTGTTAAGTC GACAAGGTTC ACTGGCATCT CCTTGCAGCC GCACATCCTC ACTTGAGGAA 
GAAGAAGAGG TTCTTGCTTC TTCACTCAAG GTCATTCCCA AGTCTGCAAA TTCCAATAGT 
GTTCTGTCCC AGGATGGCAG TCTTGCCACA ACAGGAACCA ACTATCGCCA TAGTGACCTG 
GCCAAGCTTT ATGGCATTTC AGAGGTGGTG AAAAGTGAGT GCGAGGATGA TGAAGATGAA 
GTGGATCGAG AAGATGAAAC TCCATCCTGT TCACCACCTC AGCGCCCCCA CCTTCATCAG 
ACAGGTGTGA ACAGCATGTT TAAAAACCTT GCATCTGTAC TTGAAAGTCA GAAGTACACA 
TACCGGGGTG GTCCCTTTGG ACGTCCTCCT CGTTCTGCTT TTGTGGGAGT GAAGTACTCC 
TCATCTCTTT CTTTGGGGCC TGATATATGC AGGCAGCAAC AAAGCACTTC TCCCACATCA 
GGTTCAGCCA GTCACCCAGG CTTTAGCAGT CCAACGCAAA CCTCTGCCAT TAGCAACTCA 
GGTCAGACCT GTCCCCTCTC ACCTACTAGT TGTGAATCAG AGAGTAAAAG AGGGGATAAG 
ATAAGCCCTT CAGATCTTCT GGGCAATGAT GATGAGAAGG ATATTTCAGA AGAACCCAAA 
GTTAAGGTGG ATTCTGGAAA CATGAAAGAC TTGTTGGAAA GACGACCAAA GCTGACCACC 
ATCTCAGAGT CTTCTCTAGC AGAGCTTGGT CAAAGCTTGG AAGTGAATAA ACACATAATC 
CCTTTTAAAG ATCACTCAAA AGCAGAGGCT CGTGAGATAT GCAAACCTGT TGAAGAAAAG 
GAACGACACA GAGACAGAGA ACGAGAAAGG AAACACAAAC ACAGCAGCAG TAGCAAAAAA 
CATGAGGACA AGAAAGAGAG AAAGAAAAAG CACAGAGAAA GACATGACGA TGCATCCCTT 
TCCTCCTCCT CTTCTTCCAG CTCCAGTCGA AGACACAGGG ACGGAAAGTC ACATAAGGAA 
AAGAAGAGCA GGCAAGTGCT GGGTAATCTG GACCTTCAGA GTAAGGAGCT TAGGGAGAAA 
GAACAAGAGC GAGATGGAGA CAAGAAGAGA AGGAAAGAAG AAAGTGGCCG ACCCCAGAAT 
GAAGGGGAAG CGTGGACTCA GATTAGAGAG AAGGGCAGCA GCTCTGGACA TTCTTCCGAT 
CTCCCATCAG GTTCATCGGC ATTATGTTCG GATGACTTTC AGAAACTGAA AGCACTGACA 
GATGGGCCAC CTAAAGAACT GAAGATTCGT CTCATAAAGG TGGAGAGTGG GGACAGGGAG 
ACATTTATTG CTTCAGAAGT AGAAGAGAAG AGAATTCCTC TGAAAGATAT AACCATAAAG 
AACTCTGCTA GTGAAATTAT CAGAGCCTGC AAGGCAGCTC GTGTGAAGGG GAAATTCAAG 
GAATCCTATC TTCTGGATGC CTTTTCTGTG AAACCTGTTG TGAGGACAGA GCTGCCCATT 
CCTCGTGAGA AACTCAACCC CCCTACACCA AGCATCTATT TGGAAAGTAA AAGAGATGCA 
TTCTCTCCAG TCCTGCTGCA GTTTTGTACA GATCCTAAAA ACCCCATTAC TGTCATCCGG 
GGACTAGCTG GCTCTCTCAG ATTAAATCTT GGTTTGTTTT CCACAAAGTC TTTGGTGGAG 
GCAAATTCAG AGCATGCAGT TGAGGTCCGA ACCCAGGTCC AGCAACCAGC AGATGAGAAC 
TGGGATGCCA GCGGCACCGG ACAGACCTGG CCCTGTGAGA GCAGCAGGTC ACATACAACA 
ATTGCCAAGT ATGCCCAGTA CCAGGCATCT AGCTTCCAAG AGAGCTTACA GGAAGAGAAA 
GGCAGTGACG CTGAGGAGGA TAATGATGAA AAGCCCACCA CTAAAGCGGA AAGTACAACC 
AGTAACTCTT CGGCACCCAA CTCCAGTTCA GAGCAGAAAA CTGTGGGGAA AATAATTAAA 
TTTGGCACAA ACATTGACCT GTCTGATCCC AAAAGATGGA AGCCTCAGCT GCAGGAACTG 
CAGAAGTTGC CAGCATTTAT GCGCGTATCC TCCAGTGGGA ATATGCTAAG CCATGTTGGC 
CACACCATTT TGGGAATGAA TACAGTACAA CTATATATGA AGATTCCTGG ATGCCGCACA 
CCAGGACACC AGGAGAACAA CAACTTCTGT TCTGTTAACA TCAACATTGG ACCTGGAGAC 
TGTGAGTGGT TTGCTGTCCA TGATGACTAC TGGGAGGCCA TCAGTGATTT TTGTCAGAAA 
CATGGAGTGG ACTATTTGAC GGGTTCATGG TGGCCAGTTC TGGATGACTT GTATCGCGCT 
AACATCCCTG TGTACCGTTT CATCCAAAGG CCAGGAGATC TGGTGTGGAT CAACGCAGGC 
ACTGTACACT GGGTTCAGGC CGTGGGCTGG TGCAACAACA TTGCCTGGAA TGTGGGACCC 
GTCAACGCTT ATCAGTACCA GGTGGCTTTA GAAAGATTTG AGTGGAACGA AGTGAAGAAA 
GTGAAATCCA TCGTTCCCAT GATTCATGTG TCCTGGAATG TGGCCCGCAC TGTTAAAGTC 
ACCGACCCAG ACGCTTACAA CATGATCAAA CACTGTCTCC TCCAGTCTAT TAAGCACATT 
CAGATTCTGA GGGATCAGCT GGTGGCAGCA GGAATGAAGA TTTCCTACCA GAGCAGAGTG 
AAGGATGAGC CGGCCTACTA CTGTAACGAG TGTGATGTGG AGGTGTTCAA CCTGCTGTTT 
GTGACTAGTG AGAACGGCAG TCGGAAGATG TACGTGGTGC ACTGTGAGGA CTGTGCACGT 
CAACGCAGCC CAAACCTCTC CAATGTAGTA GTGCTGGAGC AGTACCGCAT GGAGGAGCTC 
ATGAACATGT ACGACTCCTT CAGCCTGGCG GTCAGTCCCA GCTCTCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6BA ( XM_005172240.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005172297.1 Danio rerio lysine (K)-specific demethylase 6B, a (kdm6ba), transcript variant X1, mRNA.

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CloneID	ODa12856
Clone ID Related Accession (Same CDS sequence)	XM_005172239.4 , XM_021477779.1 , XM_021477780.1 , XM_009303125.2 , XM_005172240.3 , XM_017357011.1 , XM_005172240.4 , XM_009303125.3 , XM_005172239.3
Accession Version	XM_005172240.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	lysine-specific demethylase 6B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172240.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCACA CAGCAGAGCA GTTCACCGGG TGCAGCACAC GGGACCCTTT TCCTCTGGAC 
GGACTCAACC GGGGACCATG GGCTCCCGTG GGCAGCCGTG CCTGGCCACC TCCTTCCAGG 
TGCTTAACTG GTGTCAATCA GCACCAGCTT CTTCCCCATA TGCCCAGTCA CATGACTGGG 
CTAAATCATC CTAGTAAATT CTTGAACAAT GGACCCATAC ATCGAGGGGA TAAGCAAGAC 
CAGTCTCAGG CTTTGATGCC GGCATTGCAA AGAATGCCCC CTGCTCCTCC CAGGCCTTGG 
GATCCAACCA GAGCTGGCCA AGGGTATGAG CCATTGCCTG GTGATAACCA CAACAGATTG 
CGCAATAGCT ACAATGCAGG GCCTCCTTCA CACCTTACAG CTCGAGCCAA TCAGCTACTG 
AAGTTTGGTG CTCCTCATCC TCAATTGGCA ACCCATGGAT CACGGCCCAT TCATCCATTA 
CCTGATTTAT GGACTCCACC TGAAAATCAG CAGCAACCCA GGGGAACTCA CTCTAATTCT 
GGACAATTAA AACGTCCTGG TCCCCCTCTC GGAGAACATT CTGTTATTCA GCATACACCT 
CCTCCCTCTC TACACCGGCC CATAGAAGAC CTCCCCAGTC CGAGCAAGAG GAAGAAGAGC 
TCTGGGTCTG AACAGATTGC GCATTCAGCC ATGCAACGTT TATCTGGACC AACTGTACAT 
TTGAATCAAC AGCCATCCTC CCACTATCCC CCACCTAAAC CTGGCTTTTG GAACCCTTTG 
CACAAAGTGG GAGCACCCTG GAACCCAACT GAACACAAAA GTGGACCCAT TCATTTCCAG 
GATTCAGCTA AGCCAGAAGT TGAAAGTATC CCCTATAAAG CACCTCCTAT AAGTCTATCA 
ACCTTATCTT CTCCCACCAT TAACACCACA AGTGGCTATG GACAAAGCAG GGCCTCTTCA 
TCACAGTCAC AAAAGGCCAC TCAATCACCA CAGTCTCTGG GACCACCTTT ACAGAGCCCT 
CACATCCACC ATTCAGCATA CTCTTATTCC AACAACAGAC CTTCAGACCA GTCCCAAGGA 
GAACCACATG GTACTACTCC ACAAAGAGGA CATCATTTGG ATACCCCACC TCCGCTAATA 
CCTAAATTCT CAGCATCACT GCGGGCAGAT AGGGAGCGTC CTGCACCCTC ACCAGCAAAC 
CACACCACTG TGCCTTACAG TCACCCCCAG TTCCAGCCTC ACCCAGGGCT AGTCCATTCC 
ACCAGCCCAC CAGCCAGCAA CCAGGCCAAG GCCACAGTAC CTCAGCACAA CCTCCACAAA 
CCCTGGAGGA ACCAGGAGTC ACATGACTGT GCAGGAACAG GAGTTTCTCA GATCGCAGCA 
CGCCACTCTC AGGCACATAA TAGTGTTGTG GGTAACCAGG GTCCAAGGAC CCCCCAACAT 
GTGAGCCCAC CTCAGGCTTC TGCTCGAAAA CCTGTCATTA CTCCAAACCT AGAAACTCCT 
CATCCACCTT GCTCCCTGAA ACCATATACT AGTGTTGGAA GCATCCCTAC GATGAGCTCG 
GCACCCCTTA CAACCTCCTC AATCCACAGC ACTCTAAGCA TTGGAAGACC AACGGATTCT 
TCAGTGCTGA TGTCAAACCC AAACCATCTA TCCATGATTG GTCAAAATCC ACCACATCTT 
GGTTATCAGG GTGTTCACCC TGGTGTTGGA ACCCTTCCAC ATCTACATGG AGTGCAATCT 
CAAATTCAGC AGTCCACTCT TACCCACACA GGACAAAGTA GACCTAACTA CACCCCAGTG 
TCCTCTACTC CTTCCACCCT AAACTCAGGG CTTCAGAGAT CAGGGGACAG CGTCATCACC 
AGCAAACCCT CTAACACTCT CCCACAACCG GTCAGTTCAC CTCTAAGTTG CCCTCACCCT 
CCAGCACAAA TAAAGGCCAA CTCAATCTTG CAGCCAATGT CCAGCAGGTC ATACAACTCT 
GTAACCACTG AATCTTCCTC CTCAATTTCT CAGGCAACTA CTACTGTTCC AACTCCAGTT 
TACCCAGGAT CCAGTTTGCA ACCTACTGTC CCATGCTCAA TTGCTGATGC TTTTAATAAA 
CTTGATGCTG AGTTGCAGGG CCACATGCAA GCAGAAGAGA GAAAGAGACA ACAGGAAGAA 
AAGAGAAAAC AGATTGTAGA GAAACAAGAA GTGAACACAA AACCACATTG TGAGTCTGCC 
ATTAGAAGTC TGGAACGTTT GTTGTCAGGT AATACAACTG AGCCTCCCCC TCCTCGTTTG 
TCTCCAGCCA ATGCACCCTC TTCCATCTCG CCTTCTAGCC AGAATTCACC ACCTTATCCC 
TGGCTGAGTA GAGGAGGAGT GCCCCCTCGT CCTTCTGGAA TTTCAGCAAC CATTATAGAA 
CAATCACAAC CACCTCCTCT TACTCCCCAG ACAGAATATG CCCGTGAGAA GCAAAGGCAG 
AGGGAGAAAT TGAAGAGTGG GACACCATCT CAGAATTCTA CTGGGATTTC CACATACCAC 
TCAGAATCTA CTCTCATCAG CACCTCCAAC CACAACATGC AACTGAAACC TGACCCATCC 
AAAGACTTCA TGTTGAAAAC CGCTCAAAAT ATTGGGATAA TTGGTGCTAT TCCTAACCCT 
CCACCCCTGC GAGAGCAACC CAAACTTTAT CAAGCTTTTC ACAAGGATGC TAACCCTTCA 
TGCACATTAA TTAGCATGAC GGAAAACAAC CTTCAAAAAC AAATTCCCAG TGCAGGACTT 
CAAGGTCTGG GCAGCAATGC TAGTAGCTGC AGTGGTGATT CAGATGGTGC CCAATTTGAG 
GAAGAAACTT CTGAGCTTTT GCCTGATGGA TTAGCTAACA TTATGAAGAT GCTGGATGAG 
TCAATCAAGA AAGAGGAGGA GTTGTATTCT GGTCAGAAGG GAGAACAAGC AGTGTCTGAA 
CTTCAATTTT CTACAAATGT GGCTCCTGTG AAGAGTTATT TGTGCGCACC AGACCTTGTG 
CCTGCCCTAA AGCAATCACC AGCTGAGGAT TATCAGCCAG ATGGCCATGC AAGCCCCCCT 
GTGTTAAGTC GACAAGGTTC ACTGGCATCT CCTTGCAGCC GCACATCCTC ACTTGAGGAA 
GAAGAAGAGG TTCTTGCTTC TTCACTCAAG GTCATTCCCA AGTCTGCAAA TTCCAATAGT 
GTTCTGTCCC AGGATGGCAG TCTTGCCACA ACAGGAACCA ACTATCGCCA TAGTGACCTG 
GCCAAGCTTT ATGGCATTTC AGAGGTGGTG AAAAGTGAGT GCGAGGATGA TGAAGATGAA 
GTGGATCGAG AAGATGAAAC TCCATCCTGT TCACCACCTC AGCGCCCCCA CCTTCATCAG 
ACAGGTGTGA ACAGCATGTT TAAAAACCTT GCATCTGTAC TTGAAAGTCA GAAGTACACA 
TACCGGGGTG GTCCCTTTGG ACGTCCTCCT CGTTCTGCTT TTGTGGGAGT GAAGTACTCC 
TCATCTCTTT CTTTGGGGCC TGATATATGC AGGCAGCAAC AAAGCACTTC TCCCACATCA 
GGTTCAGCCA GTCACCCAGG CTTTAGCAGT CCAACGCAAA CCTCTGCCAT TAGCAACTCA 
GGTCAGACCT GTCCCCTCTC ACCTACTAGT TGTGAATCAG AGAGTAAAAG AGGGGATAAG 
ATAAGCCCTT CAGATCTTCT GGGCAATGAT GATGAGAAGG ATATTTCAGA AGAACCCAAA 
GTTAAGGTGG ATTCTGGAAA CATGAAAGAC TTGTTGGAAA GACGACCAAA GCTGACCACC 
ATCTCAGAGT CTTCTCTAGC AGAGCTTGGT CAAAGCTTGG AAGTGAATAA ACACATAATC 
CCTTTTAAAG ATCACTCAAA AGCAGAGGCT CGTGAGATAT GCAAACCTGT TGAAGAAAAG 
GAACGACACA GAGACAGAGA ACGAGAAAGG AAACACAAAC ACAGCAGCAG TAGCAAAAAA 
CATGAGGACA AGAAAGAGAG AAAGAAAAAG CACAGAGAAA GACATGACGA TGCATCCCTT 
TCCTCCTCCT CTTCTTCCAG CTCCAGTCGA AGACACAGGG ACGGAAAGTC ACATAAGGAA 
AAGAAGAGCA GGCAAGTGCT GGGTAATCTG GACCTTCAGA GTAAGGAGCT TAGGGAGAAA 
GAACAAGAGC GAGATGGAGA CAAGAAGAGA AGGAAAGAAG AAAGTGGCCG ACCCCAGAAT 
GAAGGGGAAG CGTGGACTCA GATTAGAGAG AAGGGCAGCA GCTCTGGACA TTCTTCCGAT 
CTCCCATCAG GTTCATCGGC ATTATGTTCG GATGACTTTC AGAAACTGAA AGCACTGACA 
GATGGGCCAC CTAAAGAACT GAAGATTCGT CTCATAAAGG TGGAGAGTGG GGACAGGGAG 
ACATTTATTG CTTCAGAAGT AGAAGAGAAG AGAATTCCTC TGAAAGATAT AACCATAAAG 
AACTCTGCTA GTGAAATTAT CAGAGCCTGC AAGGCAGCTC GTGTGAAGGG GAAATTCAAG 
GAATCCTATC TTCTGGATGC CTTTTCTGTG AAACCTGTTG TGAGGACAGA GCTGCCCATT 
CCTCGTGAGA AACTCAACCC CCCTACACCA AGCATCTATT TGGAAAGTAA AAGAGATGCA 
TTCTCTCCAG TCCTGCTGCA GTTTTGTACA GATCCTAAAA ACCCCATTAC TGTCATCCGG 
GGACTAGCTG GCTCTCTCAG ATTAAATCTT GGTTTGTTTT CCACAAAGTC TTTGGTGGAG 
GCAAATTCAG AGCATGCAGT TGAGGTCCGA ACCCAGGTCC AGCAACCAGC AGATGAGAAC 
TGGGATGCCA GCGGCACCGG ACAGACCTGG CCCTGTGAGA GCAGCAGGTC ACATACAACA 
ATTGCCAAGT ATGCCCAGTA CCAGGCATCT AGCTTCCAAG AGAGCTTACA GGAAGAGAAA 
GGCAGTGACG CTGAGGAGGA TAATGATGAA AAGCCCACCA CTAAAGCGGA AAGTACAACC 
AGTAACTCTT CGGCACCCAA CTCCAGTTCA GAGCAGAAAA CTGTGGGGAA AATAATTAAA 
TTTGGCACAA ACATTGACCT GTCTGATCCC AAAAGATGGA AGCCTCAGCT GCAGGAACTG 
CAGAAGTTGC CAGCATTTAT GCGCGTATCC TCCAGTGGGA ATATGCTAAG CCATGTTGGC 
CACACCATTT TGGGAATGAA TACAGTACAA CTATATATGA AGATTCCTGG ATGCCGCACA 
CCAGGACACC AGGAGAACAA CAACTTCTGT TCTGTTAACA TCAACATTGG ACCTGGAGAC 
TGTGAGTGGT TTGCTGTCCA TGATGACTAC TGGGAGGCCA TCAGTGATTT TTGTCAGAAA 
CATGGAGTGG ACTATTTGAC GGGTTCATGG TGGCCAGTTC TGGATGACTT GTATCGCGCT 
AACATCCCTG TGTACCGTTT CATCCAAAGG CCAGGAGATC TGGTGTGGAT CAACGCAGGC 
ACTGTACACT GGGTTCAGGC CGTGGGCTGG TGCAACAACA TTGCCTGGAA TGTGGGACCC 
GTCAACGCTT ATCAGTACCA GGTGGCTTTA GAAAGATTTG AGTGGAACGA AGTGAAGAAA 
GTGAAATCCA TCGTTCCCAT GATTCATGTG TCCTGGAATG TGGCCCGCAC TGTTAAAGTC 
ACCGACCCAG ACGCTTACAA CATGATCAAA CACTGTCTCC TCCAGTCTAT TAAGCACATT 
CAGATTCTGA GGGATCAGCT GGTGGCAGCA GGAATGAAGA TTTCCTACCA GAGCAGAGTG 
AAGGATGAGC CGGCCTACTA CTGTAACGAG TGTGATGTGG AGGTGTTCAA CCTGCTGTTT 
GTGACTAGTG AGAACGGCAG TCGGAAGATG TACGTGGTGC ACTGTGAGGA CTGTGCACGT 
CAACGCAGCC CAAACCTCTC CAATGTAGTA GTGCTGGAGC AGTACCGCAT GGAGGAGCTC 
ATGAACATGT ACGACTCCTT CAGCCTGGCG GTCAGTCCCA GCTCTCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172297.1
CDS	696..6686
Translation	MHHTAEQFTGCSTRDPFPLDGLNRGPWAPVGSRAWPPPSRCLTGVNQHQLLPHMPSHMTGLNHPSKFLNNGPIHRGDKQDQSQALMPALQRMPPAPPRPWDPTRAGQGYEPLPGDNHNRLRNSYNAGPPSHLTARANQLLKFGAPHPQLATHGSRPIHPLPDLWTPPENQQQPRGTHSNSGQLKRPGPPLGEHSVIQHTPPPSLHRPIEDLPSPSKRKKSSGSEQIAHSAMQRLSGPTVHLNQQPSSHYPPPKPGFWNPLHKVGAPWNPTEHKSGPIHFQDSAKPEVESIPYKAPPISLSTLSSPTINTTSGYGQSRASSSQSQKATQSPQSLGPPLQSPHIHHSAYSYSNNRPSDQSQGEPHGTTPQRGHHLDTPPPLIPKFSASLRADRERPAPSPANHTTVPYSHPQFQPHPGLVHSTSPPASNQAKATVPQHNLHKPWRNQESHDCAGTGVSQIAARHSQAHNSVVGNQGPRTPQHVSPPQASARKPVITPNLETPHPPCSLKPYTSVGSIPTMSSAPLTTSSIHSTLSIGRPTDSSVLMSNPNHLSMIGQNPPHLGYQGVHPGVGTLPHLHGVQSQIQQSTLTHTGQSRPNYTPVSSTPSTLNSGLQRSGDSVITSKPSNTLPQPVSSPLSCPHPPAQIKANSILQPMSSRSYNSVTTESSSSISQATTTVPTPVYPGSSLQPTVPCSIADAFNKLDAELQGHMQAEERKRQQEEKRKQIVEKQEVNTKPHCESAIRSLERLLSGNTTEPPPPRLSPANAPSSISPSSQNSPPYPWLSRGGVPPRPSGISATIIEQSQPPPLTPQTEYAREKQRQREKLKSGTPSQNSTGISTYHSESTLISTSNHNMQLKPDPSKDFMLKTAQNIGIIGAIPNPPPLREQPKLYQAFHKDANPSCTLISMTENNLQKQIPSAGLQGLGSNASSCSGDSDGAQFEEETSELLPDGLANIMKMLDESIKKEEELYSGQKGEQAVSELQFSTNVAPVKSYLCAPDLVPALKQSPAEDYQPDGHASPPVLSRQGSLASPCSRTSSLEEEEEVLASSLKVIPKSANSNSVLSQDGSLATTGTNYRHSDLAKLYGISEVVKSECEDDEDEVDREDETPSCSPPQRPHLHQTGVNSMFKNLASVLESQKYTYRGGPFGRPPRSAFVGVKYSSSLSLGPDICRQQQSTSPTSGSASHPGFSSPTQTSAISNSGQTCPLSPTSCESESKRGDKISPSDLLGNDDEKDISEEPKVKVDSGNMKDLLERRPKLTTISESSLAELGQSLEVNKHIIPFKDHSKAEAREICKPVEEKERHRDRERERKHKHSSSSKKHEDKKERKKKHRERHDDASLSSSSSSSSSRRHRDGKSHKEKKSRQVLGNLDLQSKELREKEQERDGDKKRRKEESGRPQNEGEAWTQIREKGSSSGHSSDLPSGSSALCSDDFQKLKALTDGPPKELKIRLIKVESGDRETFIASEVEEKRIPLKDITIKNSASEIIRACKAARVKGKFKESYLLDAFSVKPVVRTELPIPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPKNPITVIRGLAGSLRLNLGLFSTKSLVEANSEHAVEVRTQVQQPADENWDASGTGQTWPCESSRSHTTIAKYAQYQASSFQESLQEEKGSDAEEDNDEKPTTKAESTTSNSSAPNSSSEQKTVGKIIKFGTNIDLSDPKRWKPQLQELQKLPAFMRVSSSGNMLSHVGHTILGMNTVQLYMKIPGCRTPGHQENNNFCSVNINIGPGDCEWFAVHDDYWEAISDFCQKHGVDYLTGSWWPVLDDLYRANIPVYRFIQRPGDLVWINAGTVHWVQAVGWCNNIAWNVGPVNAYQYQVALERFEWNEVKKVKSIVPMIHVSWNVARTVKVTDPDAYNMIKHCLLQSIKHIQILRDQLVAAGMKISYQSRVKDEPAYYCNECDVEVFNLLFVTSENGSRKMYVVHCEDCARQRSPNLSNVVVLEQYRMEELMNMYDSFSLAVSPSSR

Target ORF information:

RefSeq Version	XM_005172240.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 6B, a (kdm6ba), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172240.4

ORF Insert Sequence:

ATGCATCACA CAGCAGAGCA GTTCACCGGG TGCAGCACAC GGGACCCTTT TCCTCTGGAC 
GGACTCAACC GGGGACCATG GGCTCCCGTG GGCAGCCGTG CCTGGCCACC TCCTTCCAGG 
TGCTTAACTG GTGTCAATCA GCACCAGCTT CTTCCCCATA TGCCCAGTCA CATGACTGGG 
CTAAATCATC CTAGTAAATT CTTGAACAAT GGACCCATAC ATCGAGGGGA TAAGCAAGAC 
CAGTCTCAGG CTTTGATGCC GGCATTGCAA AGAATGCCCC CTGCTCCTCC CAGGCCTTGG 
GATCCAACCA GAGCTGGCCA AGGGTATGAG CCATTGCCTG GTGATAACCA CAACAGATTG 
CGCAATAGCT ACAATGCAGG GCCTCCTTCA CACCTTACAG CTCGAGCCAA TCAGCTACTG 
AAGTTTGGTG CTCCTCATCC TCAATTGGCA ACCCATGGAT CACGGCCCAT TCATCCATTA 
CCTGATTTAT GGACTCCACC TGAAAATCAG CAGCAACCCA GGGGAACTCA CTCTAATTCT 
GGACAATTAA AACGTCCTGG TCCCCCTCTC GGAGAACATT CTGTTATTCA GCATACACCT 
CCTCCCTCTC TACACCGGCC CATAGAAGAC CTCCCCAGTC CGAGCAAGAG GAAGAAGAGC 
TCTGGGTCTG AACAGATTGC GCATTCAGCC ATGCAACGTT TATCTGGACC AACTGTACAT 
TTGAATCAAC AGCCATCCTC CCACTATCCC CCACCTAAAC CTGGCTTTTG GAACCCTTTG 
CACAAAGTGG GAGCACCCTG GAACCCAACT GAACACAAAA GTGGACCCAT TCATTTCCAG 
GATTCAGCTA AGCCAGAAGT TGAAAGTATC CCCTATAAAG CACCTCCTAT AAGTCTATCA 
ACCTTATCTT CTCCCACCAT TAACACCACA AGTGGCTATG GACAAAGCAG GGCCTCTTCA 
TCACAGTCAC AAAAGGCCAC TCAATCACCA CAGTCTCTGG GACCACCTTT ACAGAGCCCT 
CACATCCACC ATTCAGCATA CTCTTATTCC AACAACAGAC CTTCAGACCA GTCCCAAGGA 
GAACCACATG GTACTACTCC ACAAAGAGGA CATCATTTGG ATACCCCACC TCCGCTAATA 
CCTAAATTCT CAGCATCACT GCGGGCAGAT AGGGAGCGTC CTGCACCCTC ACCAGCAAAC 
CACACCACTG TGCCTTACAG TCACCCCCAG TTCCAGCCTC ACCCAGGGCT AGTCCATTCC 
ACCAGCCCAC CAGCCAGCAA CCAGGCCAAG GCCACAGTAC CTCAGCACAA CCTCCACAAA 
CCCTGGAGGA ACCAGGAGTC ACATGACTGT GCAGGAACAG GAGTTTCTCA GATCGCAGCA 
CGCCACTCTC AGGCACATAA TAGTGTTGTG GGTAACCAGG GTCCAAGGAC CCCCCAACAT 
GTGAGCCCAC CTCAGGCTTC TGCTCGAAAA CCTGTCATTA CTCCAAACCT AGAAACTCCT 
CATCCACCTT GCTCCCTGAA ACCATATACT AGTGTTGGAA GCATCCCTAC GATGAGCTCG 
GCACCCCTTA CAACCTCCTC AATCCACAGC ACTCTAAGCA TTGGAAGACC AACGGATTCT 
TCAGTGCTGA TGTCAAACCC AAACCATCTA TCCATGATTG GTCAAAATCC ACCACATCTT 
GGTTATCAGG GTGTTCACCC TGGTGTTGGA ACCCTTCCAC ATCTACATGG AGTGCAATCT 
CAAATTCAGC AGTCCACTCT TACCCACACA GGACAAAGTA GACCTAACTA CACCCCAGTG 
TCCTCTACTC CTTCCACCCT AAACTCAGGG CTTCAGAGAT CAGGGGACAG CGTCATCACC 
AGCAAACCCT CTAACACTCT CCCACAACCG GTCAGTTCAC CTCTAAGTTG CCCTCACCCT 
CCAGCACAAA TAAAGGCCAA CTCAATCTTG CAGCCAATGT CCAGCAGGTC ATACAACTCT 
GTAACCACTG AATCTTCCTC CTCAATTTCT CAGGCAACTA CTACTGTTCC AACTCCAGTT 
TACCCAGGAT CCAGTTTGCA ACCTACTGTC CCATGCTCAA TTGCTGATGC TTTTAATAAA 
CTTGATGCTG AGTTGCAGGG CCACATGCAA GCAGAAGAGA GAAAGAGACA ACAGGAAGAA 
AAGAGAAAAC AGATTGTAGA GAAACAAGAA GTGAACACAA AACCACATTG TGAGTCTGCC 
ATTAGAAGTC TGGAACGTTT GTTGTCAGGT AATACAACTG AGCCTCCCCC TCCTCGTTTG 
TCTCCAGCCA ATGCACCCTC TTCCATCTCG CCTTCTAGCC AGAATTCACC ACCTTATCCC 
TGGCTGAGTA GAGGAGGAGT GCCCCCTCGT CCTTCTGGAA TTTCAGCAAC CATTATAGAA 
CAATCACAAC CACCTCCTCT TACTCCCCAG ACAGAATATG CCCGTGAGAA GCAAAGGCAG 
AGGGAGAAAT TGAAGAGTGG GACACCATCT CAGAATTCTA CTGGGATTTC CACATACCAC 
TCAGAATCTA CTCTCATCAG CACCTCCAAC CACAACATGC AACTGAAACC TGACCCATCC 
AAAGACTTCA TGTTGAAAAC CGCTCAAAAT ATTGGGATAA TTGGTGCTAT TCCTAACCCT 
CCACCCCTGC GAGAGCAACC CAAACTTTAT CAAGCTTTTC ACAAGGATGC TAACCCTTCA 
TGCACATTAA TTAGCATGAC GGAAAACAAC CTTCAAAAAC AAATTCCCAG TGCAGGACTT 
CAAGGTCTGG GCAGCAATGC TAGTAGCTGC AGTGGTGATT CAGATGGTGC CCAATTTGAG 
GAAGAAACTT CTGAGCTTTT GCCTGATGGA TTAGCTAACA TTATGAAGAT GCTGGATGAG 
TCAATCAAGA AAGAGGAGGA GTTGTATTCT GGTCAGAAGG GAGAACAAGC AGTGTCTGAA 
CTTCAATTTT CTACAAATGT GGCTCCTGTG AAGAGTTATT TGTGCGCACC AGACCTTGTG 
CCTGCCCTAA AGCAATCACC AGCTGAGGAT TATCAGCCAG ATGGCCATGC AAGCCCCCCT 
GTGTTAAGTC GACAAGGTTC ACTGGCATCT CCTTGCAGCC GCACATCCTC ACTTGAGGAA 
GAAGAAGAGG TTCTTGCTTC TTCACTCAAG GTCATTCCCA AGTCTGCAAA TTCCAATAGT 
GTTCTGTCCC AGGATGGCAG TCTTGCCACA ACAGGAACCA ACTATCGCCA TAGTGACCTG 
GCCAAGCTTT ATGGCATTTC AGAGGTGGTG AAAAGTGAGT GCGAGGATGA TGAAGATGAA 
GTGGATCGAG AAGATGAAAC TCCATCCTGT TCACCACCTC AGCGCCCCCA CCTTCATCAG 
ACAGGTGTGA ACAGCATGTT TAAAAACCTT GCATCTGTAC TTGAAAGTCA GAAGTACACA 
TACCGGGGTG GTCCCTTTGG ACGTCCTCCT CGTTCTGCTT TTGTGGGAGT GAAGTACTCC 
TCATCTCTTT CTTTGGGGCC TGATATATGC AGGCAGCAAC AAAGCACTTC TCCCACATCA 
GGTTCAGCCA GTCACCCAGG CTTTAGCAGT CCAACGCAAA CCTCTGCCAT TAGCAACTCA 
GGTCAGACCT GTCCCCTCTC ACCTACTAGT TGTGAATCAG AGAGTAAAAG AGGGGATAAG 
ATAAGCCCTT CAGATCTTCT GGGCAATGAT GATGAGAAGG ATATTTCAGA AGAACCCAAA 
GTTAAGGTGG ATTCTGGAAA CATGAAAGAC TTGTTGGAAA GACGACCAAA GCTGACCACC 
ATCTCAGAGT CTTCTCTAGC AGAGCTTGGT CAAAGCTTGG AAGTGAATAA ACACATAATC 
CCTTTTAAAG ATCACTCAAA AGCAGAGGCT CGTGAGATAT GCAAACCTGT TGAAGAAAAG 
GAACGACACA GAGACAGAGA ACGAGAAAGG AAACACAAAC ACAGCAGCAG TAGCAAAAAA 
CATGAGGACA AGAAAGAGAG AAAGAAAAAG CACAGAGAAA GACATGACGA TGCATCCCTT 
TCCTCCTCCT CTTCTTCCAG CTCCAGTCGA AGACACAGGG ACGGAAAGTC ACATAAGGAA 
AAGAAGAGCA GGCAAGTGCT GGGTAATCTG GACCTTCAGA GTAAGGAGCT TAGGGAGAAA 
GAACAAGAGC GAGATGGAGA CAAGAAGAGA AGGAAAGAAG AAAGTGGCCG ACCCCAGAAT 
GAAGGGGAAG CGTGGACTCA GATTAGAGAG AAGGGCAGCA GCTCTGGACA TTCTTCCGAT 
CTCCCATCAG GTTCATCGGC ATTATGTTCG GATGACTTTC AGAAACTGAA AGCACTGACA 
GATGGGCCAC CTAAAGAACT GAAGATTCGT CTCATAAAGG TGGAGAGTGG GGACAGGGAG 
ACATTTATTG CTTCAGAAGT AGAAGAGAAG AGAATTCCTC TGAAAGATAT AACCATAAAG 
AACTCTGCTA GTGAAATTAT CAGAGCCTGC AAGGCAGCTC GTGTGAAGGG GAAATTCAAG 
GAATCCTATC TTCTGGATGC CTTTTCTGTG AAACCTGTTG TGAGGACAGA GCTGCCCATT 
CCTCGTGAGA AACTCAACCC CCCTACACCA AGCATCTATT TGGAAAGTAA AAGAGATGCA 
TTCTCTCCAG TCCTGCTGCA GTTTTGTACA GATCCTAAAA ACCCCATTAC TGTCATCCGG 
GGACTAGCTG GCTCTCTCAG ATTAAATCTT GGTTTGTTTT CCACAAAGTC TTTGGTGGAG 
GCAAATTCAG AGCATGCAGT TGAGGTCCGA ACCCAGGTCC AGCAACCAGC AGATGAGAAC 
TGGGATGCCA GCGGCACCGG ACAGACCTGG CCCTGTGAGA GCAGCAGGTC ACATACAACA 
ATTGCCAAGT ATGCCCAGTA CCAGGCATCT AGCTTCCAAG AGAGCTTACA GGAAGAGAAA 
GGCAGTGACG CTGAGGAGGA TAATGATGAA AAGCCCACCA CTAAAGCGGA AAGTACAACC 
AGTAACTCTT CGGCACCCAA CTCCAGTTCA GAGCAGAAAA CTGTGGGGAA AATAATTAAA 
TTTGGCACAA ACATTGACCT GTCTGATCCC AAAAGATGGA AGCCTCAGCT GCAGGAACTG 
CAGAAGTTGC CAGCATTTAT GCGCGTATCC TCCAGTGGGA ATATGCTAAG CCATGTTGGC 
CACACCATTT TGGGAATGAA TACAGTACAA CTATATATGA AGATTCCTGG ATGCCGCACA 
CCAGGACACC AGGAGAACAA CAACTTCTGT TCTGTTAACA TCAACATTGG ACCTGGAGAC 
TGTGAGTGGT TTGCTGTCCA TGATGACTAC TGGGAGGCCA TCAGTGATTT TTGTCAGAAA 
CATGGAGTGG ACTATTTGAC GGGTTCATGG TGGCCAGTTC TGGATGACTT GTATCGCGCT 
AACATCCCTG TGTACCGTTT CATCCAAAGG CCAGGAGATC TGGTGTGGAT CAACGCAGGC 
ACTGTACACT GGGTTCAGGC CGTGGGCTGG TGCAACAACA TTGCCTGGAA TGTGGGACCC 
GTCAACGCTT ATCAGTACCA GGTGGCTTTA GAAAGATTTG AGTGGAACGA AGTGAAGAAA 
GTGAAATCCA TCGTTCCCAT GATTCATGTG TCCTGGAATG TGGCCCGCAC TGTTAAAGTC 
ACCGACCCAG ACGCTTACAA CATGATCAAA CACTGTCTCC TCCAGTCTAT TAAGCACATT 
CAGATTCTGA GGGATCAGCT GGTGGCAGCA GGAATGAAGA TTTCCTACCA GAGCAGAGTG 
AAGGATGAGC CGGCCTACTA CTGTAACGAG TGTGATGTGG AGGTGTTCAA CCTGCTGTTT 
GTGACTAGTG AGAACGGCAG TCGGAAGATG TACGTGGTGC ACTGTGAGGA CTGTGCACGT 
CAACGCAGCC CAAACCTCTC CAATGTAGTA GTGCTGGAGC AGTACCGCAT GGAGGAGCTC 
ATGAACATGT ACGACTCCTT CAGCCTGGCG GTCAGTCCCA GCTCTCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6BA ( XM_009303125.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009301400.1 Danio rerio lysine (K)-specific demethylase 6B, a (kdm6ba), transcript variant X2, mRNA.

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CloneID	ODa12856
Clone ID Related Accession (Same CDS sequence)	XM_005172239.4 , XM_021477779.1 , XM_021477780.1 , XM_009303125.2 , XM_005172240.3 , XM_017357011.1 , XM_005172240.4 , XM_009303125.3 , XM_005172239.3
Accession Version	XM_009303125.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	lysine-specific demethylase 6B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303125.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCACA CAGCAGAGCA GTTCACCGGG TGCAGCACAC GGGACCCTTT TCCTCTGGAC 
GGACTCAACC GGGGACCATG GGCTCCCGTG GGCAGCCGTG CCTGGCCACC TCCTTCCAGG 
TGCTTAACTG GTGTCAATCA GCACCAGCTT CTTCCCCATA TGCCCAGTCA CATGACTGGG 
CTAAATCATC CTAGTAAATT CTTGAACAAT GGACCCATAC ATCGAGGGGA TAAGCAAGAC 
CAGTCTCAGG CTTTGATGCC GGCATTGCAA AGAATGCCCC CTGCTCCTCC CAGGCCTTGG 
GATCCAACCA GAGCTGGCCA AGGGTATGAG CCATTGCCTG GTGATAACCA CAACAGATTG 
CGCAATAGCT ACAATGCAGG GCCTCCTTCA CACCTTACAG CTCGAGCCAA TCAGCTACTG 
AAGTTTGGTG CTCCTCATCC TCAATTGGCA ACCCATGGAT CACGGCCCAT TCATCCATTA 
CCTGATTTAT GGACTCCACC TGAAAATCAG CAGCAACCCA GGGGAACTCA CTCTAATTCT 
GGACAATTAA AACGTCCTGG TCCCCCTCTC GGAGAACATT CTGTTATTCA GCATACACCT 
CCTCCCTCTC TACACCGGCC CATAGAAGAC CTCCCCAGTC CGAGCAAGAG GAAGAAGAGC 
TCTGGGTCTG AACAGATTGC GCATTCAGCC ATGCAACGTT TATCTGGACC AACTGTACAT 
TTGAATCAAC AGCCATCCTC CCACTATCCC CCACCTAAAC CTGGCTTTTG GAACCCTTTG 
CACAAAGTGG GAGCACCCTG GAACCCAACT GAACACAAAA GTGGACCCAT TCATTTCCAG 
GATTCAGCTA AGCCAGAAGT TGAAAGTATC CCCTATAAAG CACCTCCTAT AAGTCTATCA 
ACCTTATCTT CTCCCACCAT TAACACCACA AGTGGCTATG GACAAAGCAG GGCCTCTTCA 
TCACAGTCAC AAAAGGCCAC TCAATCACCA CAGTCTCTGG GACCACCTTT ACAGAGCCCT 
CACATCCACC ATTCAGCATA CTCTTATTCC AACAACAGAC CTTCAGACCA GTCCCAAGGA 
GAACCACATG GTACTACTCC ACAAAGAGGA CATCATTTGG ATACCCCACC TCCGCTAATA 
CCTAAATTCT CAGCATCACT GCGGGCAGAT AGGGAGCGTC CTGCACCCTC ACCAGCAAAC 
CACACCACTG TGCCTTACAG TCACCCCCAG TTCCAGCCTC ACCCAGGGCT AGTCCATTCC 
ACCAGCCCAC CAGCCAGCAA CCAGGCCAAG GCCACAGTAC CTCAGCACAA CCTCCACAAA 
CCCTGGAGGA ACCAGGAGTC ACATGACTGT GCAGGAACAG GAGTTTCTCA GATCGCAGCA 
CGCCACTCTC AGGCACATAA TAGTGTTGTG GGTAACCAGG GTCCAAGGAC CCCCCAACAT 
GTGAGCCCAC CTCAGGCTTC TGCTCGAAAA CCTGTCATTA CTCCAAACCT AGAAACTCCT 
CATCCACCTT GCTCCCTGAA ACCATATACT AGTGTTGGAA GCATCCCTAC GATGAGCTCG 
GCACCCCTTA CAACCTCCTC AATCCACAGC ACTCTAAGCA TTGGAAGACC AACGGATTCT 
TCAGTGCTGA TGTCAAACCC AAACCATCTA TCCATGATTG GTCAAAATCC ACCACATCTT 
GGTTATCAGG GTGTTCACCC TGGTGTTGGA ACCCTTCCAC ATCTACATGG AGTGCAATCT 
CAAATTCAGC AGTCCACTCT TACCCACACA GGACAAAGTA GACCTAACTA CACCCCAGTG 
TCCTCTACTC CTTCCACCCT AAACTCAGGG CTTCAGAGAT CAGGGGACAG CGTCATCACC 
AGCAAACCCT CTAACACTCT CCCACAACCG GTCAGTTCAC CTCTAAGTTG CCCTCACCCT 
CCAGCACAAA TAAAGGCCAA CTCAATCTTG CAGCCAATGT CCAGCAGGTC ATACAACTCT 
GTAACCACTG AATCTTCCTC CTCAATTTCT CAGGCAACTA CTACTGTTCC AACTCCAGTT 
TACCCAGGAT CCAGTTTGCA ACCTACTGTC CCATGCTCAA TTGCTGATGC TTTTAATAAA 
CTTGATGCTG AGTTGCAGGG CCACATGCAA GCAGAAGAGA GAAAGAGACA ACAGGAAGAA 
AAGAGAAAAC AGATTGTAGA GAAACAAGAA GTGAACACAA AACCACATTG TGAGTCTGCC 
ATTAGAAGTC TGGAACGTTT GTTGTCAGGT AATACAACTG AGCCTCCCCC TCCTCGTTTG 
TCTCCAGCCA ATGCACCCTC TTCCATCTCG CCTTCTAGCC AGAATTCACC ACCTTATCCC 
TGGCTGAGTA GAGGAGGAGT GCCCCCTCGT CCTTCTGGAA TTTCAGCAAC CATTATAGAA 
CAATCACAAC CACCTCCTCT TACTCCCCAG ACAGAATATG CCCGTGAGAA GCAAAGGCAG 
AGGGAGAAAT TGAAGAGTGG GACACCATCT CAGAATTCTA CTGGGATTTC CACATACCAC 
TCAGAATCTA CTCTCATCAG CACCTCCAAC CACAACATGC AACTGAAACC TGACCCATCC 
AAAGACTTCA TGTTGAAAAC CGCTCAAAAT ATTGGGATAA TTGGTGCTAT TCCTAACCCT 
CCACCCCTGC GAGAGCAACC CAAACTTTAT CAAGCTTTTC ACAAGGATGC TAACCCTTCA 
TGCACATTAA TTAGCATGAC GGAAAACAAC CTTCAAAAAC AAATTCCCAG TGCAGGACTT 
CAAGGTCTGG GCAGCAATGC TAGTAGCTGC AGTGGTGATT CAGATGGTGC CCAATTTGAG 
GAAGAAACTT CTGAGCTTTT GCCTGATGGA TTAGCTAACA TTATGAAGAT GCTGGATGAG 
TCAATCAAGA AAGAGGAGGA GTTGTATTCT GGTCAGAAGG GAGAACAAGC AGTGTCTGAA 
CTTCAATTTT CTACAAATGT GGCTCCTGTG AAGAGTTATT TGTGCGCACC AGACCTTGTG 
CCTGCCCTAA AGCAATCACC AGCTGAGGAT TATCAGCCAG ATGGCCATGC AAGCCCCCCT 
GTGTTAAGTC GACAAGGTTC ACTGGCATCT CCTTGCAGCC GCACATCCTC ACTTGAGGAA 
GAAGAAGAGG TTCTTGCTTC TTCACTCAAG GTCATTCCCA AGTCTGCAAA TTCCAATAGT 
GTTCTGTCCC AGGATGGCAG TCTTGCCACA ACAGGAACCA ACTATCGCCA TAGTGACCTG 
GCCAAGCTTT ATGGCATTTC AGAGGTGGTG AAAAGTGAGT GCGAGGATGA TGAAGATGAA 
GTGGATCGAG AAGATGAAAC TCCATCCTGT TCACCACCTC AGCGCCCCCA CCTTCATCAG 
ACAGGTGTGA ACAGCATGTT TAAAAACCTT GCATCTGTAC TTGAAAGTCA GAAGTACACA 
TACCGGGGTG GTCCCTTTGG ACGTCCTCCT CGTTCTGCTT TTGTGGGAGT GAAGTACTCC 
TCATCTCTTT CTTTGGGGCC TGATATATGC AGGCAGCAAC AAAGCACTTC TCCCACATCA 
GGTTCAGCCA GTCACCCAGG CTTTAGCAGT CCAACGCAAA CCTCTGCCAT TAGCAACTCA 
GGTCAGACCT GTCCCCTCTC ACCTACTAGT TGTGAATCAG AGAGTAAAAG AGGGGATAAG 
ATAAGCCCTT CAGATCTTCT GGGCAATGAT GATGAGAAGG ATATTTCAGA AGAACCCAAA 
GTTAAGGTGG ATTCTGGAAA CATGAAAGAC TTGTTGGAAA GACGACCAAA GCTGACCACC 
ATCTCAGAGT CTTCTCTAGC AGAGCTTGGT CAAAGCTTGG AAGTGAATAA ACACATAATC 
CCTTTTAAAG ATCACTCAAA AGCAGAGGCT CGTGAGATAT GCAAACCTGT TGAAGAAAAG 
GAACGACACA GAGACAGAGA ACGAGAAAGG AAACACAAAC ACAGCAGCAG TAGCAAAAAA 
CATGAGGACA AGAAAGAGAG AAAGAAAAAG CACAGAGAAA GACATGACGA TGCATCCCTT 
TCCTCCTCCT CTTCTTCCAG CTCCAGTCGA AGACACAGGG ACGGAAAGTC ACATAAGGAA 
AAGAAGAGCA GGCAAGTGCT GGGTAATCTG GACCTTCAGA GTAAGGAGCT TAGGGAGAAA 
GAACAAGAGC GAGATGGAGA CAAGAAGAGA AGGAAAGAAG AAAGTGGCCG ACCCCAGAAT 
GAAGGGGAAG CGTGGACTCA GATTAGAGAG AAGGGCAGCA GCTCTGGACA TTCTTCCGAT 
CTCCCATCAG GTTCATCGGC ATTATGTTCG GATGACTTTC AGAAACTGAA AGCACTGACA 
GATGGGCCAC CTAAAGAACT GAAGATTCGT CTCATAAAGG TGGAGAGTGG GGACAGGGAG 
ACATTTATTG CTTCAGAAGT AGAAGAGAAG AGAATTCCTC TGAAAGATAT AACCATAAAG 
AACTCTGCTA GTGAAATTAT CAGAGCCTGC AAGGCAGCTC GTGTGAAGGG GAAATTCAAG 
GAATCCTATC TTCTGGATGC CTTTTCTGTG AAACCTGTTG TGAGGACAGA GCTGCCCATT 
CCTCGTGAGA AACTCAACCC CCCTACACCA AGCATCTATT TGGAAAGTAA AAGAGATGCA 
TTCTCTCCAG TCCTGCTGCA GTTTTGTACA GATCCTAAAA ACCCCATTAC TGTCATCCGG 
GGACTAGCTG GCTCTCTCAG ATTAAATCTT GGTTTGTTTT CCACAAAGTC TTTGGTGGAG 
GCAAATTCAG AGCATGCAGT TGAGGTCCGA ACCCAGGTCC AGCAACCAGC AGATGAGAAC 
TGGGATGCCA GCGGCACCGG ACAGACCTGG CCCTGTGAGA GCAGCAGGTC ACATACAACA 
ATTGCCAAGT ATGCCCAGTA CCAGGCATCT AGCTTCCAAG AGAGCTTACA GGAAGAGAAA 
GGCAGTGACG CTGAGGAGGA TAATGATGAA AAGCCCACCA CTAAAGCGGA AAGTACAACC 
AGTAACTCTT CGGCACCCAA CTCCAGTTCA GAGCAGAAAA CTGTGGGGAA AATAATTAAA 
TTTGGCACAA ACATTGACCT GTCTGATCCC AAAAGATGGA AGCCTCAGCT GCAGGAACTG 
CAGAAGTTGC CAGCATTTAT GCGCGTATCC TCCAGTGGGA ATATGCTAAG CCATGTTGGC 
CACACCATTT TGGGAATGAA TACAGTACAA CTATATATGA AGATTCCTGG ATGCCGCACA 
CCAGGACACC AGGAGAACAA CAACTTCTGT TCTGTTAACA TCAACATTGG ACCTGGAGAC 
TGTGAGTGGT TTGCTGTCCA TGATGACTAC TGGGAGGCCA TCAGTGATTT TTGTCAGAAA 
CATGGAGTGG ACTATTTGAC GGGTTCATGG TGGCCAGTTC TGGATGACTT GTATCGCGCT 
AACATCCCTG TGTACCGTTT CATCCAAAGG CCAGGAGATC TGGTGTGGAT CAACGCAGGC 
ACTGTACACT GGGTTCAGGC CGTGGGCTGG TGCAACAACA TTGCCTGGAA TGTGGGACCC 
GTCAACGCTT ATCAGTACCA GGTGGCTTTA GAAAGATTTG AGTGGAACGA AGTGAAGAAA 
GTGAAATCCA TCGTTCCCAT GATTCATGTG TCCTGGAATG TGGCCCGCAC TGTTAAAGTC 
ACCGACCCAG ACGCTTACAA CATGATCAAA CACTGTCTCC TCCAGTCTAT TAAGCACATT 
CAGATTCTGA GGGATCAGCT GGTGGCAGCA GGAATGAAGA TTTCCTACCA GAGCAGAGTG 
AAGGATGAGC CGGCCTACTA CTGTAACGAG TGTGATGTGG AGGTGTTCAA CCTGCTGTTT 
GTGACTAGTG AGAACGGCAG TCGGAAGATG TACGTGGTGC ACTGTGAGGA CTGTGCACGT 
CAACGCAGCC CAAACCTCTC CAATGTAGTA GTGCTGGAGC AGTACCGCAT GGAGGAGCTC 
ATGAACATGT ACGACTCCTT CAGCCTGGCG GTCAGTCCCA GCTCTCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301400.1
CDS	916..6906
Translation	MHHTAEQFTGCSTRDPFPLDGLNRGPWAPVGSRAWPPPSRCLTGVNQHQLLPHMPSHMTGLNHPSKFLNNGPIHRGDKQDQSQALMPALQRMPPAPPRPWDPTRAGQGYEPLPGDNHNRLRNSYNAGPPSHLTARANQLLKFGAPHPQLATHGSRPIHPLPDLWTPPENQQQPRGTHSNSGQLKRPGPPLGEHSVIQHTPPPSLHRPIEDLPSPSKRKKSSGSEQIAHSAMQRLSGPTVHLNQQPSSHYPPPKPGFWNPLHKVGAPWNPTEHKSGPIHFQDSAKPEVESIPYKAPPISLSTLSSPTINTTSGYGQSRASSSQSQKATQSPQSLGPPLQSPHIHHSAYSYSNNRPSDQSQGEPHGTTPQRGHHLDTPPPLIPKFSASLRADRERPAPSPANHTTVPYSHPQFQPHPGLVHSTSPPASNQAKATVPQHNLHKPWRNQESHDCAGTGVSQIAARHSQAHNSVVGNQGPRTPQHVSPPQASARKPVITPNLETPHPPCSLKPYTSVGSIPTMSSAPLTTSSIHSTLSIGRPTDSSVLMSNPNHLSMIGQNPPHLGYQGVHPGVGTLPHLHGVQSQIQQSTLTHTGQSRPNYTPVSSTPSTLNSGLQRSGDSVITSKPSNTLPQPVSSPLSCPHPPAQIKANSILQPMSSRSYNSVTTESSSSISQATTTVPTPVYPGSSLQPTVPCSIADAFNKLDAELQGHMQAEERKRQQEEKRKQIVEKQEVNTKPHCESAIRSLERLLSGNTTEPPPPRLSPANAPSSISPSSQNSPPYPWLSRGGVPPRPSGISATIIEQSQPPPLTPQTEYAREKQRQREKLKSGTPSQNSTGISTYHSESTLISTSNHNMQLKPDPSKDFMLKTAQNIGIIGAIPNPPPLREQPKLYQAFHKDANPSCTLISMTENNLQKQIPSAGLQGLGSNASSCSGDSDGAQFEEETSELLPDGLANIMKMLDESIKKEEELYSGQKGEQAVSELQFSTNVAPVKSYLCAPDLVPALKQSPAEDYQPDGHASPPVLSRQGSLASPCSRTSSLEEEEEVLASSLKVIPKSANSNSVLSQDGSLATTGTNYRHSDLAKLYGISEVVKSECEDDEDEVDREDETPSCSPPQRPHLHQTGVNSMFKNLASVLESQKYTYRGGPFGRPPRSAFVGVKYSSSLSLGPDICRQQQSTSPTSGSASHPGFSSPTQTSAISNSGQTCPLSPTSCESESKRGDKISPSDLLGNDDEKDISEEPKVKVDSGNMKDLLERRPKLTTISESSLAELGQSLEVNKHIIPFKDHSKAEAREICKPVEEKERHRDRERERKHKHSSSSKKHEDKKERKKKHRERHDDASLSSSSSSSSSRRHRDGKSHKEKKSRQVLGNLDLQSKELREKEQERDGDKKRRKEESGRPQNEGEAWTQIREKGSSSGHSSDLPSGSSALCSDDFQKLKALTDGPPKELKIRLIKVESGDRETFIASEVEEKRIPLKDITIKNSASEIIRACKAARVKGKFKESYLLDAFSVKPVVRTELPIPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPKNPITVIRGLAGSLRLNLGLFSTKSLVEANSEHAVEVRTQVQQPADENWDASGTGQTWPCESSRSHTTIAKYAQYQASSFQESLQEEKGSDAEEDNDEKPTTKAESTTSNSSAPNSSSEQKTVGKIIKFGTNIDLSDPKRWKPQLQELQKLPAFMRVSSSGNMLSHVGHTILGMNTVQLYMKIPGCRTPGHQENNNFCSVNINIGPGDCEWFAVHDDYWEAISDFCQKHGVDYLTGSWWPVLDDLYRANIPVYRFIQRPGDLVWINAGTVHWVQAVGWCNNIAWNVGPVNAYQYQVALERFEWNEVKKVKSIVPMIHVSWNVARTVKVTDPDAYNMIKHCLLQSIKHIQILRDQLVAAGMKISYQSRVKDEPAYYCNECDVEVFNLLFVTSENGSRKMYVVHCEDCARQRSPNLSNVVVLEQYRMEELMNMYDSFSLAVSPSSR

Target ORF information:

RefSeq Version	XM_009303125.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 6B, a (kdm6ba), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303125.3

ORF Insert Sequence:

ATGCATCACA CAGCAGAGCA GTTCACCGGG TGCAGCACAC GGGACCCTTT TCCTCTGGAC 
GGACTCAACC GGGGACCATG GGCTCCCGTG GGCAGCCGTG CCTGGCCACC TCCTTCCAGG 
TGCTTAACTG GTGTCAATCA GCACCAGCTT CTTCCCCATA TGCCCAGTCA CATGACTGGG 
CTAAATCATC CTAGTAAATT CTTGAACAAT GGACCCATAC ATCGAGGGGA TAAGCAAGAC 
CAGTCTCAGG CTTTGATGCC GGCATTGCAA AGAATGCCCC CTGCTCCTCC CAGGCCTTGG 
GATCCAACCA GAGCTGGCCA AGGGTATGAG CCATTGCCTG GTGATAACCA CAACAGATTG 
CGCAATAGCT ACAATGCAGG GCCTCCTTCA CACCTTACAG CTCGAGCCAA TCAGCTACTG 
AAGTTTGGTG CTCCTCATCC TCAATTGGCA ACCCATGGAT CACGGCCCAT TCATCCATTA 
CCTGATTTAT GGACTCCACC TGAAAATCAG CAGCAACCCA GGGGAACTCA CTCTAATTCT 
GGACAATTAA AACGTCCTGG TCCCCCTCTC GGAGAACATT CTGTTATTCA GCATACACCT 
CCTCCCTCTC TACACCGGCC CATAGAAGAC CTCCCCAGTC CGAGCAAGAG GAAGAAGAGC 
TCTGGGTCTG AACAGATTGC GCATTCAGCC ATGCAACGTT TATCTGGACC AACTGTACAT 
TTGAATCAAC AGCCATCCTC CCACTATCCC CCACCTAAAC CTGGCTTTTG GAACCCTTTG 
CACAAAGTGG GAGCACCCTG GAACCCAACT GAACACAAAA GTGGACCCAT TCATTTCCAG 
GATTCAGCTA AGCCAGAAGT TGAAAGTATC CCCTATAAAG CACCTCCTAT AAGTCTATCA 
ACCTTATCTT CTCCCACCAT TAACACCACA AGTGGCTATG GACAAAGCAG GGCCTCTTCA 
TCACAGTCAC AAAAGGCCAC TCAATCACCA CAGTCTCTGG GACCACCTTT ACAGAGCCCT 
CACATCCACC ATTCAGCATA CTCTTATTCC AACAACAGAC CTTCAGACCA GTCCCAAGGA 
GAACCACATG GTACTACTCC ACAAAGAGGA CATCATTTGG ATACCCCACC TCCGCTAATA 
CCTAAATTCT CAGCATCACT GCGGGCAGAT AGGGAGCGTC CTGCACCCTC ACCAGCAAAC 
CACACCACTG TGCCTTACAG TCACCCCCAG TTCCAGCCTC ACCCAGGGCT AGTCCATTCC 
ACCAGCCCAC CAGCCAGCAA CCAGGCCAAG GCCACAGTAC CTCAGCACAA CCTCCACAAA 
CCCTGGAGGA ACCAGGAGTC ACATGACTGT GCAGGAACAG GAGTTTCTCA GATCGCAGCA 
CGCCACTCTC AGGCACATAA TAGTGTTGTG GGTAACCAGG GTCCAAGGAC CCCCCAACAT 
GTGAGCCCAC CTCAGGCTTC TGCTCGAAAA CCTGTCATTA CTCCAAACCT AGAAACTCCT 
CATCCACCTT GCTCCCTGAA ACCATATACT AGTGTTGGAA GCATCCCTAC GATGAGCTCG 
GCACCCCTTA CAACCTCCTC AATCCACAGC ACTCTAAGCA TTGGAAGACC AACGGATTCT 
TCAGTGCTGA TGTCAAACCC AAACCATCTA TCCATGATTG GTCAAAATCC ACCACATCTT 
GGTTATCAGG GTGTTCACCC TGGTGTTGGA ACCCTTCCAC ATCTACATGG AGTGCAATCT 
CAAATTCAGC AGTCCACTCT TACCCACACA GGACAAAGTA GACCTAACTA CACCCCAGTG 
TCCTCTACTC CTTCCACCCT AAACTCAGGG CTTCAGAGAT CAGGGGACAG CGTCATCACC 
AGCAAACCCT CTAACACTCT CCCACAACCG GTCAGTTCAC CTCTAAGTTG CCCTCACCCT 
CCAGCACAAA TAAAGGCCAA CTCAATCTTG CAGCCAATGT CCAGCAGGTC ATACAACTCT 
GTAACCACTG AATCTTCCTC CTCAATTTCT CAGGCAACTA CTACTGTTCC AACTCCAGTT 
TACCCAGGAT CCAGTTTGCA ACCTACTGTC CCATGCTCAA TTGCTGATGC TTTTAATAAA 
CTTGATGCTG AGTTGCAGGG CCACATGCAA GCAGAAGAGA GAAAGAGACA ACAGGAAGAA 
AAGAGAAAAC AGATTGTAGA GAAACAAGAA GTGAACACAA AACCACATTG TGAGTCTGCC 
ATTAGAAGTC TGGAACGTTT GTTGTCAGGT AATACAACTG AGCCTCCCCC TCCTCGTTTG 
TCTCCAGCCA ATGCACCCTC TTCCATCTCG CCTTCTAGCC AGAATTCACC ACCTTATCCC 
TGGCTGAGTA GAGGAGGAGT GCCCCCTCGT CCTTCTGGAA TTTCAGCAAC CATTATAGAA 
CAATCACAAC CACCTCCTCT TACTCCCCAG ACAGAATATG CCCGTGAGAA GCAAAGGCAG 
AGGGAGAAAT TGAAGAGTGG GACACCATCT CAGAATTCTA CTGGGATTTC CACATACCAC 
TCAGAATCTA CTCTCATCAG CACCTCCAAC CACAACATGC AACTGAAACC TGACCCATCC 
AAAGACTTCA TGTTGAAAAC CGCTCAAAAT ATTGGGATAA TTGGTGCTAT TCCTAACCCT 
CCACCCCTGC GAGAGCAACC CAAACTTTAT CAAGCTTTTC ACAAGGATGC TAACCCTTCA 
TGCACATTAA TTAGCATGAC GGAAAACAAC CTTCAAAAAC AAATTCCCAG TGCAGGACTT 
CAAGGTCTGG GCAGCAATGC TAGTAGCTGC AGTGGTGATT CAGATGGTGC CCAATTTGAG 
GAAGAAACTT CTGAGCTTTT GCCTGATGGA TTAGCTAACA TTATGAAGAT GCTGGATGAG 
TCAATCAAGA AAGAGGAGGA GTTGTATTCT GGTCAGAAGG GAGAACAAGC AGTGTCTGAA 
CTTCAATTTT CTACAAATGT GGCTCCTGTG AAGAGTTATT TGTGCGCACC AGACCTTGTG 
CCTGCCCTAA AGCAATCACC AGCTGAGGAT TATCAGCCAG ATGGCCATGC AAGCCCCCCT 
GTGTTAAGTC GACAAGGTTC ACTGGCATCT CCTTGCAGCC GCACATCCTC ACTTGAGGAA 
GAAGAAGAGG TTCTTGCTTC TTCACTCAAG GTCATTCCCA AGTCTGCAAA TTCCAATAGT 
GTTCTGTCCC AGGATGGCAG TCTTGCCACA ACAGGAACCA ACTATCGCCA TAGTGACCTG 
GCCAAGCTTT ATGGCATTTC AGAGGTGGTG AAAAGTGAGT GCGAGGATGA TGAAGATGAA 
GTGGATCGAG AAGATGAAAC TCCATCCTGT TCACCACCTC AGCGCCCCCA CCTTCATCAG 
ACAGGTGTGA ACAGCATGTT TAAAAACCTT GCATCTGTAC TTGAAAGTCA GAAGTACACA 
TACCGGGGTG GTCCCTTTGG ACGTCCTCCT CGTTCTGCTT TTGTGGGAGT GAAGTACTCC 
TCATCTCTTT CTTTGGGGCC TGATATATGC AGGCAGCAAC AAAGCACTTC TCCCACATCA 
GGTTCAGCCA GTCACCCAGG CTTTAGCAGT CCAACGCAAA CCTCTGCCAT TAGCAACTCA 
GGTCAGACCT GTCCCCTCTC ACCTACTAGT TGTGAATCAG AGAGTAAAAG AGGGGATAAG 
ATAAGCCCTT CAGATCTTCT GGGCAATGAT GATGAGAAGG ATATTTCAGA AGAACCCAAA 
GTTAAGGTGG ATTCTGGAAA CATGAAAGAC TTGTTGGAAA GACGACCAAA GCTGACCACC 
ATCTCAGAGT CTTCTCTAGC AGAGCTTGGT CAAAGCTTGG AAGTGAATAA ACACATAATC 
CCTTTTAAAG ATCACTCAAA AGCAGAGGCT CGTGAGATAT GCAAACCTGT TGAAGAAAAG 
GAACGACACA GAGACAGAGA ACGAGAAAGG AAACACAAAC ACAGCAGCAG TAGCAAAAAA 
CATGAGGACA AGAAAGAGAG AAAGAAAAAG CACAGAGAAA GACATGACGA TGCATCCCTT 
TCCTCCTCCT CTTCTTCCAG CTCCAGTCGA AGACACAGGG ACGGAAAGTC ACATAAGGAA 
AAGAAGAGCA GGCAAGTGCT GGGTAATCTG GACCTTCAGA GTAAGGAGCT TAGGGAGAAA 
GAACAAGAGC GAGATGGAGA CAAGAAGAGA AGGAAAGAAG AAAGTGGCCG ACCCCAGAAT 
GAAGGGGAAG CGTGGACTCA GATTAGAGAG AAGGGCAGCA GCTCTGGACA TTCTTCCGAT 
CTCCCATCAG GTTCATCGGC ATTATGTTCG GATGACTTTC AGAAACTGAA AGCACTGACA 
GATGGGCCAC CTAAAGAACT GAAGATTCGT CTCATAAAGG TGGAGAGTGG GGACAGGGAG 
ACATTTATTG CTTCAGAAGT AGAAGAGAAG AGAATTCCTC TGAAAGATAT AACCATAAAG 
AACTCTGCTA GTGAAATTAT CAGAGCCTGC AAGGCAGCTC GTGTGAAGGG GAAATTCAAG 
GAATCCTATC TTCTGGATGC CTTTTCTGTG AAACCTGTTG TGAGGACAGA GCTGCCCATT 
CCTCGTGAGA AACTCAACCC CCCTACACCA AGCATCTATT TGGAAAGTAA AAGAGATGCA 
TTCTCTCCAG TCCTGCTGCA GTTTTGTACA GATCCTAAAA ACCCCATTAC TGTCATCCGG 
GGACTAGCTG GCTCTCTCAG ATTAAATCTT GGTTTGTTTT CCACAAAGTC TTTGGTGGAG 
GCAAATTCAG AGCATGCAGT TGAGGTCCGA ACCCAGGTCC AGCAACCAGC AGATGAGAAC 
TGGGATGCCA GCGGCACCGG ACAGACCTGG CCCTGTGAGA GCAGCAGGTC ACATACAACA 
ATTGCCAAGT ATGCCCAGTA CCAGGCATCT AGCTTCCAAG AGAGCTTACA GGAAGAGAAA 
GGCAGTGACG CTGAGGAGGA TAATGATGAA AAGCCCACCA CTAAAGCGGA AAGTACAACC 
AGTAACTCTT CGGCACCCAA CTCCAGTTCA GAGCAGAAAA CTGTGGGGAA AATAATTAAA 
TTTGGCACAA ACATTGACCT GTCTGATCCC AAAAGATGGA AGCCTCAGCT GCAGGAACTG 
CAGAAGTTGC CAGCATTTAT GCGCGTATCC TCCAGTGGGA ATATGCTAAG CCATGTTGGC 
CACACCATTT TGGGAATGAA TACAGTACAA CTATATATGA AGATTCCTGG ATGCCGCACA 
CCAGGACACC AGGAGAACAA CAACTTCTGT TCTGTTAACA TCAACATTGG ACCTGGAGAC 
TGTGAGTGGT TTGCTGTCCA TGATGACTAC TGGGAGGCCA TCAGTGATTT TTGTCAGAAA 
CATGGAGTGG ACTATTTGAC GGGTTCATGG TGGCCAGTTC TGGATGACTT GTATCGCGCT 
AACATCCCTG TGTACCGTTT CATCCAAAGG CCAGGAGATC TGGTGTGGAT CAACGCAGGC 
ACTGTACACT GGGTTCAGGC CGTGGGCTGG TGCAACAACA TTGCCTGGAA TGTGGGACCC 
GTCAACGCTT ATCAGTACCA GGTGGCTTTA GAAAGATTTG AGTGGAACGA AGTGAAGAAA 
GTGAAATCCA TCGTTCCCAT GATTCATGTG TCCTGGAATG TGGCCCGCAC TGTTAAAGTC 
ACCGACCCAG ACGCTTACAA CATGATCAAA CACTGTCTCC TCCAGTCTAT TAAGCACATT 
CAGATTCTGA GGGATCAGCT GGTGGCAGCA GGAATGAAGA TTTCCTACCA GAGCAGAGTG 
AAGGATGAGC CGGCCTACTA CTGTAACGAG TGTGATGTGG AGGTGTTCAA CCTGCTGTTT 
GTGACTAGTG AGAACGGCAG TCGGAAGATG TACGTGGTGC ACTGTGAGGA CTGTGCACGT 
CAACGCAGCC CAAACCTCTC CAATGTAGTA GTGCTGGAGC AGTACCGCAT GGAGGAGCTC 
ATGAACATGT ACGACTCCTT CAGCCTGGCG GTCAGTCCCA GCTCTCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6BA ( XM_005172239.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005172296.1 Danio rerio lysine (K)-specific demethylase 6B, a (kdm6ba), transcript variant X2, mRNA.

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CloneID	ODa12856
Clone ID Related Accession (Same CDS sequence)	XM_005172239.4 , XM_021477779.1 , XM_021477780.1 , XM_009303125.2 , XM_005172240.3 , XM_017357011.1 , XM_005172240.4 , XM_009303125.3 , XM_005172239.3
Accession Version	XM_005172239.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	lysine-specific demethylase 6B
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172239.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCACA CAGCAGAGCA GTTCACCGGG TGCAGCACAC GGGACCCTTT TCCTCTGGAC 
GGACTCAACC GGGGACCATG GGCTCCCGTG GGCAGCCGTG CCTGGCCACC TCCTTCCAGG 
TGCTTAACTG GTGTCAATCA GCACCAGCTT CTTCCCCATA TGCCCAGTCA CATGACTGGG 
CTAAATCATC CTAGTAAATT CTTGAACAAT GGACCCATAC ATCGAGGGGA TAAGCAAGAC 
CAGTCTCAGG CTTTGATGCC GGCATTGCAA AGAATGCCCC CTGCTCCTCC CAGGCCTTGG 
GATCCAACCA GAGCTGGCCA AGGGTATGAG CCATTGCCTG GTGATAACCA CAACAGATTG 
CGCAATAGCT ACAATGCAGG GCCTCCTTCA CACCTTACAG CTCGAGCCAA TCAGCTACTG 
AAGTTTGGTG CTCCTCATCC TCAATTGGCA ACCCATGGAT CACGGCCCAT TCATCCATTA 
CCTGATTTAT GGACTCCACC TGAAAATCAG CAGCAACCCA GGGGAACTCA CTCTAATTCT 
GGACAATTAA AACGTCCTGG TCCCCCTCTC GGAGAACATT CTGTTATTCA GCATACACCT 
CCTCCCTCTC TACACCGGCC CATAGAAGAC CTCCCCAGTC CGAGCAAGAG GAAGAAGAGC 
TCTGGGTCTG AACAGATTGC GCATTCAGCC ATGCAACGTT TATCTGGACC AACTGTACAT 
TTGAATCAAC AGCCATCCTC CCACTATCCC CCACCTAAAC CTGGCTTTTG GAACCCTTTG 
CACAAAGTGG GAGCACCCTG GAACCCAACT GAACACAAAA GTGGACCCAT TCATTTCCAG 
GATTCAGCTA AGCCAGAAGT TGAAAGTATC CCCTATAAAG CACCTCCTAT AAGTCTATCA 
ACCTTATCTT CTCCCACCAT TAACACCACA AGTGGCTATG GACAAAGCAG GGCCTCTTCA 
TCACAGTCAC AAAAGGCCAC TCAATCACCA CAGTCTCTGG GACCACCTTT ACAGAGCCCT 
CACATCCACC ATTCAGCATA CTCTTATTCC AACAACAGAC CTTCAGACCA GTCCCAAGGA 
GAACCACATG GTACTACTCC ACAAAGAGGA CATCATTTGG ATACCCCACC TCCGCTAATA 
CCTAAATTCT CAGCATCACT GCGGGCAGAT AGGGAGCGTC CTGCACCCTC ACCAGCAAAC 
CACACCACTG TGCCTTACAG TCACCCCCAG TTCCAGCCTC ACCCAGGGCT AGTCCATTCC 
ACCAGCCCAC CAGCCAGCAA CCAGGCCAAG GCCACAGTAC CTCAGCACAA CCTCCACAAA 
CCCTGGAGGA ACCAGGAGTC ACATGACTGT GCAGGAACAG GAGTTTCTCA GATCGCAGCA 
CGCCACTCTC AGGCACATAA TAGTGTTGTG GGTAACCAGG GTCCAAGGAC CCCCCAACAT 
GTGAGCCCAC CTCAGGCTTC TGCTCGAAAA CCTGTCATTA CTCCAAACCT AGAAACTCCT 
CATCCACCTT GCTCCCTGAA ACCATATACT AGTGTTGGAA GCATCCCTAC GATGAGCTCG 
GCACCCCTTA CAACCTCCTC AATCCACAGC ACTCTAAGCA TTGGAAGACC AACGGATTCT 
TCAGTGCTGA TGTCAAACCC AAACCATCTA TCCATGATTG GTCAAAATCC ACCACATCTT 
GGTTATCAGG GTGTTCACCC TGGTGTTGGA ACCCTTCCAC ATCTACATGG AGTGCAATCT 
CAAATTCAGC AGTCCACTCT TACCCACACA GGACAAAGTA GACCTAACTA CACCCCAGTG 
TCCTCTACTC CTTCCACCCT AAACTCAGGG CTTCAGAGAT CAGGGGACAG CGTCATCACC 
AGCAAACCCT CTAACACTCT CCCACAACCG GTCAGTTCAC CTCTAAGTTG CCCTCACCCT 
CCAGCACAAA TAAAGGCCAA CTCAATCTTG CAGCCAATGT CCAGCAGGTC ATACAACTCT 
GTAACCACTG AATCTTCCTC CTCAATTTCT CAGGCAACTA CTACTGTTCC AACTCCAGTT 
TACCCAGGAT CCAGTTTGCA ACCTACTGTC CCATGCTCAA TTGCTGATGC TTTTAATAAA 
CTTGATGCTG AGTTGCAGGG CCACATGCAA GCAGAAGAGA GAAAGAGACA ACAGGAAGAA 
AAGAGAAAAC AGATTGTAGA GAAACAAGAA GTGAACACAA AACCACATTG TGAGTCTGCC 
ATTAGAAGTC TGGAACGTTT GTTGTCAGGT AATACAACTG AGCCTCCCCC TCCTCGTTTG 
TCTCCAGCCA ATGCACCCTC TTCCATCTCG CCTTCTAGCC AGAATTCACC ACCTTATCCC 
TGGCTGAGTA GAGGAGGAGT GCCCCCTCGT CCTTCTGGAA TTTCAGCAAC CATTATAGAA 
CAATCACAAC CACCTCCTCT TACTCCCCAG ACAGAATATG CCCGTGAGAA GCAAAGGCAG 
AGGGAGAAAT TGAAGAGTGG GACACCATCT CAGAATTCTA CTGGGATTTC CACATACCAC 
TCAGAATCTA CTCTCATCAG CACCTCCAAC CACAACATGC AACTGAAACC TGACCCATCC 
AAAGACTTCA TGTTGAAAAC CGCTCAAAAT ATTGGGATAA TTGGTGCTAT TCCTAACCCT 
CCACCCCTGC GAGAGCAACC CAAACTTTAT CAAGCTTTTC ACAAGGATGC TAACCCTTCA 
TGCACATTAA TTAGCATGAC GGAAAACAAC CTTCAAAAAC AAATTCCCAG TGCAGGACTT 
CAAGGTCTGG GCAGCAATGC TAGTAGCTGC AGTGGTGATT CAGATGGTGC CCAATTTGAG 
GAAGAAACTT CTGAGCTTTT GCCTGATGGA TTAGCTAACA TTATGAAGAT GCTGGATGAG 
TCAATCAAGA AAGAGGAGGA GTTGTATTCT GGTCAGAAGG GAGAACAAGC AGTGTCTGAA 
CTTCAATTTT CTACAAATGT GGCTCCTGTG AAGAGTTATT TGTGCGCACC AGACCTTGTG 
CCTGCCCTAA AGCAATCACC AGCTGAGGAT TATCAGCCAG ATGGCCATGC AAGCCCCCCT 
GTGTTAAGTC GACAAGGTTC ACTGGCATCT CCTTGCAGCC GCACATCCTC ACTTGAGGAA 
GAAGAAGAGG TTCTTGCTTC TTCACTCAAG GTCATTCCCA AGTCTGCAAA TTCCAATAGT 
GTTCTGTCCC AGGATGGCAG TCTTGCCACA ACAGGAACCA ACTATCGCCA TAGTGACCTG 
GCCAAGCTTT ATGGCATTTC AGAGGTGGTG AAAAGTGAGT GCGAGGATGA TGAAGATGAA 
GTGGATCGAG AAGATGAAAC TCCATCCTGT TCACCACCTC AGCGCCCCCA CCTTCATCAG 
ACAGGTGTGA ACAGCATGTT TAAAAACCTT GCATCTGTAC TTGAAAGTCA GAAGTACACA 
TACCGGGGTG GTCCCTTTGG ACGTCCTCCT CGTTCTGCTT TTGTGGGAGT GAAGTACTCC 
TCATCTCTTT CTTTGGGGCC TGATATATGC AGGCAGCAAC AAAGCACTTC TCCCACATCA 
GGTTCAGCCA GTCACCCAGG CTTTAGCAGT CCAACGCAAA CCTCTGCCAT TAGCAACTCA 
GGTCAGACCT GTCCCCTCTC ACCTACTAGT TGTGAATCAG AGAGTAAAAG AGGGGATAAG 
ATAAGCCCTT CAGATCTTCT GGGCAATGAT GATGAGAAGG ATATTTCAGA AGAACCCAAA 
GTTAAGGTGG ATTCTGGAAA CATGAAAGAC TTGTTGGAAA GACGACCAAA GCTGACCACC 
ATCTCAGAGT CTTCTCTAGC AGAGCTTGGT CAAAGCTTGG AAGTGAATAA ACACATAATC 
CCTTTTAAAG ATCACTCAAA AGCAGAGGCT CGTGAGATAT GCAAACCTGT TGAAGAAAAG 
GAACGACACA GAGACAGAGA ACGAGAAAGG AAACACAAAC ACAGCAGCAG TAGCAAAAAA 
CATGAGGACA AGAAAGAGAG AAAGAAAAAG CACAGAGAAA GACATGACGA TGCATCCCTT 
TCCTCCTCCT CTTCTTCCAG CTCCAGTCGA AGACACAGGG ACGGAAAGTC ACATAAGGAA 
AAGAAGAGCA GGCAAGTGCT GGGTAATCTG GACCTTCAGA GTAAGGAGCT TAGGGAGAAA 
GAACAAGAGC GAGATGGAGA CAAGAAGAGA AGGAAAGAAG AAAGTGGCCG ACCCCAGAAT 
GAAGGGGAAG CGTGGACTCA GATTAGAGAG AAGGGCAGCA GCTCTGGACA TTCTTCCGAT 
CTCCCATCAG GTTCATCGGC ATTATGTTCG GATGACTTTC AGAAACTGAA AGCACTGACA 
GATGGGCCAC CTAAAGAACT GAAGATTCGT CTCATAAAGG TGGAGAGTGG GGACAGGGAG 
ACATTTATTG CTTCAGAAGT AGAAGAGAAG AGAATTCCTC TGAAAGATAT AACCATAAAG 
AACTCTGCTA GTGAAATTAT CAGAGCCTGC AAGGCAGCTC GTGTGAAGGG GAAATTCAAG 
GAATCCTATC TTCTGGATGC CTTTTCTGTG AAACCTGTTG TGAGGACAGA GCTGCCCATT 
CCTCGTGAGA AACTCAACCC CCCTACACCA AGCATCTATT TGGAAAGTAA AAGAGATGCA 
TTCTCTCCAG TCCTGCTGCA GTTTTGTACA GATCCTAAAA ACCCCATTAC TGTCATCCGG 
GGACTAGCTG GCTCTCTCAG ATTAAATCTT GGTTTGTTTT CCACAAAGTC TTTGGTGGAG 
GCAAATTCAG AGCATGCAGT TGAGGTCCGA ACCCAGGTCC AGCAACCAGC AGATGAGAAC 
TGGGATGCCA GCGGCACCGG ACAGACCTGG CCCTGTGAGA GCAGCAGGTC ACATACAACA 
ATTGCCAAGT ATGCCCAGTA CCAGGCATCT AGCTTCCAAG AGAGCTTACA GGAAGAGAAA 
GGCAGTGACG CTGAGGAGGA TAATGATGAA AAGCCCACCA CTAAAGCGGA AAGTACAACC 
AGTAACTCTT CGGCACCCAA CTCCAGTTCA GAGCAGAAAA CTGTGGGGAA AATAATTAAA 
TTTGGCACAA ACATTGACCT GTCTGATCCC AAAAGATGGA AGCCTCAGCT GCAGGAACTG 
CAGAAGTTGC CAGCATTTAT GCGCGTATCC TCCAGTGGGA ATATGCTAAG CCATGTTGGC 
CACACCATTT TGGGAATGAA TACAGTACAA CTATATATGA AGATTCCTGG ATGCCGCACA 
CCAGGACACC AGGAGAACAA CAACTTCTGT TCTGTTAACA TCAACATTGG ACCTGGAGAC 
TGTGAGTGGT TTGCTGTCCA TGATGACTAC TGGGAGGCCA TCAGTGATTT TTGTCAGAAA 
CATGGAGTGG ACTATTTGAC GGGTTCATGG TGGCCAGTTC TGGATGACTT GTATCGCGCT 
AACATCCCTG TGTACCGTTT CATCCAAAGG CCAGGAGATC TGGTGTGGAT CAACGCAGGC 
ACTGTACACT GGGTTCAGGC CGTGGGCTGG TGCAACAACA TTGCCTGGAA TGTGGGACCC 
GTCAACGCTT ATCAGTACCA GGTGGCTTTA GAAAGATTTG AGTGGAACGA AGTGAAGAAA 
GTGAAATCCA TCGTTCCCAT GATTCATGTG TCCTGGAATG TGGCCCGCAC TGTTAAAGTC 
ACCGACCCAG ACGCTTACAA CATGATCAAA CACTGTCTCC TCCAGTCTAT TAAGCACATT 
CAGATTCTGA GGGATCAGCT GGTGGCAGCA GGAATGAAGA TTTCCTACCA GAGCAGAGTG 
AAGGATGAGC CGGCCTACTA CTGTAACGAG TGTGATGTGG AGGTGTTCAA CCTGCTGTTT 
GTGACTAGTG AGAACGGCAG TCGGAAGATG TACGTGGTGC ACTGTGAGGA CTGTGCACGT 
CAACGCAGCC CAAACCTCTC CAATGTAGTA GTGCTGGAGC AGTACCGCAT GGAGGAGCTC 
ATGAACATGT ACGACTCCTT CAGCCTGGCG GTCAGTCCCA GCTCTCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172296.1
CDS	546..6536
Translation	MHHTAEQFTGCSTRDPFPLDGLNRGPWAPVGSRAWPPPSRCLTGVNQHQLLPHMPSHMTGLNHPSKFLNNGPIHRGDKQDQSQALMPALQRMPPAPPRPWDPTRAGQGYEPLPGDNHNRLRNSYNAGPPSHLTARANQLLKFGAPHPQLATHGSRPIHPLPDLWTPPENQQQPRGTHSNSGQLKRPGPPLGEHSVIQHTPPPSLHRPIEDLPSPSKRKKSSGSEQIAHSAMQRLSGPTVHLNQQPSSHYPPPKPGFWNPLHKVGAPWNPTEHKSGPIHFQDSAKPEVESIPYKAPPISLSTLSSPTINTTSGYGQSRASSSQSQKATQSPQSLGPPLQSPHIHHSAYSYSNNRPSDQSQGEPHGTTPQRGHHLDTPPPLIPKFSASLRADRERPAPSPANHTTVPYSHPQFQPHPGLVHSTSPPASNQAKATVPQHNLHKPWRNQESHDCAGTGVSQIAARHSQAHNSVVGNQGPRTPQHVSPPQASARKPVITPNLETPHPPCSLKPYTSVGSIPTMSSAPLTTSSIHSTLSIGRPTDSSVLMSNPNHLSMIGQNPPHLGYQGVHPGVGTLPHLHGVQSQIQQSTLTHTGQSRPNYTPVSSTPSTLNSGLQRSGDSVITSKPSNTLPQPVSSPLSCPHPPAQIKANSILQPMSSRSYNSVTTESSSSISQATTTVPTPVYPGSSLQPTVPCSIADAFNKLDAELQGHMQAEERKRQQEEKRKQIVEKQEVNTKPHCESAIRSLERLLSGNTTEPPPPRLSPANAPSSISPSSQNSPPYPWLSRGGVPPRPSGISATIIEQSQPPPLTPQTEYAREKQRQREKLKSGTPSQNSTGISTYHSESTLISTSNHNMQLKPDPSKDFMLKTAQNIGIIGAIPNPPPLREQPKLYQAFHKDANPSCTLISMTENNLQKQIPSAGLQGLGSNASSCSGDSDGAQFEEETSELLPDGLANIMKMLDESIKKEEELYSGQKGEQAVSELQFSTNVAPVKSYLCAPDLVPALKQSPAEDYQPDGHASPPVLSRQGSLASPCSRTSSLEEEEEVLASSLKVIPKSANSNSVLSQDGSLATTGTNYRHSDLAKLYGISEVVKSECEDDEDEVDREDETPSCSPPQRPHLHQTGVNSMFKNLASVLESQKYTYRGGPFGRPPRSAFVGVKYSSSLSLGPDICRQQQSTSPTSGSASHPGFSSPTQTSAISNSGQTCPLSPTSCESESKRGDKISPSDLLGNDDEKDISEEPKVKVDSGNMKDLLERRPKLTTISESSLAELGQSLEVNKHIIPFKDHSKAEAREICKPVEEKERHRDRERERKHKHSSSSKKHEDKKERKKKHRERHDDASLSSSSSSSSSRRHRDGKSHKEKKSRQVLGNLDLQSKELREKEQERDGDKKRRKEESGRPQNEGEAWTQIREKGSSSGHSSDLPSGSSALCSDDFQKLKALTDGPPKELKIRLIKVESGDRETFIASEVEEKRIPLKDITIKNSASEIIRACKAARVKGKFKESYLLDAFSVKPVVRTELPIPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPKNPITVIRGLAGSLRLNLGLFSTKSLVEANSEHAVEVRTQVQQPADENWDASGTGQTWPCESSRSHTTIAKYAQYQASSFQESLQEEKGSDAEEDNDEKPTTKAESTTSNSSAPNSSSEQKTVGKIIKFGTNIDLSDPKRWKPQLQELQKLPAFMRVSSSGNMLSHVGHTILGMNTVQLYMKIPGCRTPGHQENNNFCSVNINIGPGDCEWFAVHDDYWEAISDFCQKHGVDYLTGSWWPVLDDLYRANIPVYRFIQRPGDLVWINAGTVHWVQAVGWCNNIAWNVGPVNAYQYQVALERFEWNEVKKVKSIVPMIHVSWNVARTVKVTDPDAYNMIKHCLLQSIKHIQILRDQLVAAGMKISYQSRVKDEPAYYCNECDVEVFNLLFVTSENGSRKMYVVHCEDCARQRSPNLSNVVVLEQYRMEELMNMYDSFSLAVSPSSR

Target ORF information:

RefSeq Version	XM_005172239.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 6B, a (kdm6ba), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172239.3

ORF Insert Sequence:

ATGCATCACA CAGCAGAGCA GTTCACCGGG TGCAGCACAC GGGACCCTTT TCCTCTGGAC 
GGACTCAACC GGGGACCATG GGCTCCCGTG GGCAGCCGTG CCTGGCCACC TCCTTCCAGG 
TGCTTAACTG GTGTCAATCA GCACCAGCTT CTTCCCCATA TGCCCAGTCA CATGACTGGG 
CTAAATCATC CTAGTAAATT CTTGAACAAT GGACCCATAC ATCGAGGGGA TAAGCAAGAC 
CAGTCTCAGG CTTTGATGCC GGCATTGCAA AGAATGCCCC CTGCTCCTCC CAGGCCTTGG 
GATCCAACCA GAGCTGGCCA AGGGTATGAG CCATTGCCTG GTGATAACCA CAACAGATTG 
CGCAATAGCT ACAATGCAGG GCCTCCTTCA CACCTTACAG CTCGAGCCAA TCAGCTACTG 
AAGTTTGGTG CTCCTCATCC TCAATTGGCA ACCCATGGAT CACGGCCCAT TCATCCATTA 
CCTGATTTAT GGACTCCACC TGAAAATCAG CAGCAACCCA GGGGAACTCA CTCTAATTCT 
GGACAATTAA AACGTCCTGG TCCCCCTCTC GGAGAACATT CTGTTATTCA GCATACACCT 
CCTCCCTCTC TACACCGGCC CATAGAAGAC CTCCCCAGTC CGAGCAAGAG GAAGAAGAGC 
TCTGGGTCTG AACAGATTGC GCATTCAGCC ATGCAACGTT TATCTGGACC AACTGTACAT 
TTGAATCAAC AGCCATCCTC CCACTATCCC CCACCTAAAC CTGGCTTTTG GAACCCTTTG 
CACAAAGTGG GAGCACCCTG GAACCCAACT GAACACAAAA GTGGACCCAT TCATTTCCAG 
GATTCAGCTA AGCCAGAAGT TGAAAGTATC CCCTATAAAG CACCTCCTAT AAGTCTATCA 
ACCTTATCTT CTCCCACCAT TAACACCACA AGTGGCTATG GACAAAGCAG GGCCTCTTCA 
TCACAGTCAC AAAAGGCCAC TCAATCACCA CAGTCTCTGG GACCACCTTT ACAGAGCCCT 
CACATCCACC ATTCAGCATA CTCTTATTCC AACAACAGAC CTTCAGACCA GTCCCAAGGA 
GAACCACATG GTACTACTCC ACAAAGAGGA CATCATTTGG ATACCCCACC TCCGCTAATA 
CCTAAATTCT CAGCATCACT GCGGGCAGAT AGGGAGCGTC CTGCACCCTC ACCAGCAAAC 
CACACCACTG TGCCTTACAG TCACCCCCAG TTCCAGCCTC ACCCAGGGCT AGTCCATTCC 
ACCAGCCCAC CAGCCAGCAA CCAGGCCAAG GCCACAGTAC CTCAGCACAA CCTCCACAAA 
CCCTGGAGGA ACCAGGAGTC ACATGACTGT GCAGGAACAG GAGTTTCTCA GATCGCAGCA 
CGCCACTCTC AGGCACATAA TAGTGTTGTG GGTAACCAGG GTCCAAGGAC CCCCCAACAT 
GTGAGCCCAC CTCAGGCTTC TGCTCGAAAA CCTGTCATTA CTCCAAACCT AGAAACTCCT 
CATCCACCTT GCTCCCTGAA ACCATATACT AGTGTTGGAA GCATCCCTAC GATGAGCTCG 
GCACCCCTTA CAACCTCCTC AATCCACAGC ACTCTAAGCA TTGGAAGACC AACGGATTCT 
TCAGTGCTGA TGTCAAACCC AAACCATCTA TCCATGATTG GTCAAAATCC ACCACATCTT 
GGTTATCAGG GTGTTCACCC TGGTGTTGGA ACCCTTCCAC ATCTACATGG AGTGCAATCT 
CAAATTCAGC AGTCCACTCT TACCCACACA GGACAAAGTA GACCTAACTA CACCCCAGTG 
TCCTCTACTC CTTCCACCCT AAACTCAGGG CTTCAGAGAT CAGGGGACAG CGTCATCACC 
AGCAAACCCT CTAACACTCT CCCACAACCG GTCAGTTCAC CTCTAAGTTG CCCTCACCCT 
CCAGCACAAA TAAAGGCCAA CTCAATCTTG CAGCCAATGT CCAGCAGGTC ATACAACTCT 
GTAACCACTG AATCTTCCTC CTCAATTTCT CAGGCAACTA CTACTGTTCC AACTCCAGTT 
TACCCAGGAT CCAGTTTGCA ACCTACTGTC CCATGCTCAA TTGCTGATGC TTTTAATAAA 
CTTGATGCTG AGTTGCAGGG CCACATGCAA GCAGAAGAGA GAAAGAGACA ACAGGAAGAA 
AAGAGAAAAC AGATTGTAGA GAAACAAGAA GTGAACACAA AACCACATTG TGAGTCTGCC 
ATTAGAAGTC TGGAACGTTT GTTGTCAGGT AATACAACTG AGCCTCCCCC TCCTCGTTTG 
TCTCCAGCCA ATGCACCCTC TTCCATCTCG CCTTCTAGCC AGAATTCACC ACCTTATCCC 
TGGCTGAGTA GAGGAGGAGT GCCCCCTCGT CCTTCTGGAA TTTCAGCAAC CATTATAGAA 
CAATCACAAC CACCTCCTCT TACTCCCCAG ACAGAATATG CCCGTGAGAA GCAAAGGCAG 
AGGGAGAAAT TGAAGAGTGG GACACCATCT CAGAATTCTA CTGGGATTTC CACATACCAC 
TCAGAATCTA CTCTCATCAG CACCTCCAAC CACAACATGC AACTGAAACC TGACCCATCC 
AAAGACTTCA TGTTGAAAAC CGCTCAAAAT ATTGGGATAA TTGGTGCTAT TCCTAACCCT 
CCACCCCTGC GAGAGCAACC CAAACTTTAT CAAGCTTTTC ACAAGGATGC TAACCCTTCA 
TGCACATTAA TTAGCATGAC GGAAAACAAC CTTCAAAAAC AAATTCCCAG TGCAGGACTT 
CAAGGTCTGG GCAGCAATGC TAGTAGCTGC AGTGGTGATT CAGATGGTGC CCAATTTGAG 
GAAGAAACTT CTGAGCTTTT GCCTGATGGA TTAGCTAACA TTATGAAGAT GCTGGATGAG 
TCAATCAAGA AAGAGGAGGA GTTGTATTCT GGTCAGAAGG GAGAACAAGC AGTGTCTGAA 
CTTCAATTTT CTACAAATGT GGCTCCTGTG AAGAGTTATT TGTGCGCACC AGACCTTGTG 
CCTGCCCTAA AGCAATCACC AGCTGAGGAT TATCAGCCAG ATGGCCATGC AAGCCCCCCT 
GTGTTAAGTC GACAAGGTTC ACTGGCATCT CCTTGCAGCC GCACATCCTC ACTTGAGGAA 
GAAGAAGAGG TTCTTGCTTC TTCACTCAAG GTCATTCCCA AGTCTGCAAA TTCCAATAGT 
GTTCTGTCCC AGGATGGCAG TCTTGCCACA ACAGGAACCA ACTATCGCCA TAGTGACCTG 
GCCAAGCTTT ATGGCATTTC AGAGGTGGTG AAAAGTGAGT GCGAGGATGA TGAAGATGAA 
GTGGATCGAG AAGATGAAAC TCCATCCTGT TCACCACCTC AGCGCCCCCA CCTTCATCAG 
ACAGGTGTGA ACAGCATGTT TAAAAACCTT GCATCTGTAC TTGAAAGTCA GAAGTACACA 
TACCGGGGTG GTCCCTTTGG ACGTCCTCCT CGTTCTGCTT TTGTGGGAGT GAAGTACTCC 
TCATCTCTTT CTTTGGGGCC TGATATATGC AGGCAGCAAC AAAGCACTTC TCCCACATCA 
GGTTCAGCCA GTCACCCAGG CTTTAGCAGT CCAACGCAAA CCTCTGCCAT TAGCAACTCA 
GGTCAGACCT GTCCCCTCTC ACCTACTAGT TGTGAATCAG AGAGTAAAAG AGGGGATAAG 
ATAAGCCCTT CAGATCTTCT GGGCAATGAT GATGAGAAGG ATATTTCAGA AGAACCCAAA 
GTTAAGGTGG ATTCTGGAAA CATGAAAGAC TTGTTGGAAA GACGACCAAA GCTGACCACC 
ATCTCAGAGT CTTCTCTAGC AGAGCTTGGT CAAAGCTTGG AAGTGAATAA ACACATAATC 
CCTTTTAAAG ATCACTCAAA AGCAGAGGCT CGTGAGATAT GCAAACCTGT TGAAGAAAAG 
GAACGACACA GAGACAGAGA ACGAGAAAGG AAACACAAAC ACAGCAGCAG TAGCAAAAAA 
CATGAGGACA AGAAAGAGAG AAAGAAAAAG CACAGAGAAA GACATGACGA TGCATCCCTT 
TCCTCCTCCT CTTCTTCCAG CTCCAGTCGA AGACACAGGG ACGGAAAGTC ACATAAGGAA 
AAGAAGAGCA GGCAAGTGCT GGGTAATCTG GACCTTCAGA GTAAGGAGCT TAGGGAGAAA 
GAACAAGAGC GAGATGGAGA CAAGAAGAGA AGGAAAGAAG AAAGTGGCCG ACCCCAGAAT 
GAAGGGGAAG CGTGGACTCA GATTAGAGAG AAGGGCAGCA GCTCTGGACA TTCTTCCGAT 
CTCCCATCAG GTTCATCGGC ATTATGTTCG GATGACTTTC AGAAACTGAA AGCACTGACA 
GATGGGCCAC CTAAAGAACT GAAGATTCGT CTCATAAAGG TGGAGAGTGG GGACAGGGAG 
ACATTTATTG CTTCAGAAGT AGAAGAGAAG AGAATTCCTC TGAAAGATAT AACCATAAAG 
AACTCTGCTA GTGAAATTAT CAGAGCCTGC AAGGCAGCTC GTGTGAAGGG GAAATTCAAG 
GAATCCTATC TTCTGGATGC CTTTTCTGTG AAACCTGTTG TGAGGACAGA GCTGCCCATT 
CCTCGTGAGA AACTCAACCC CCCTACACCA AGCATCTATT TGGAAAGTAA AAGAGATGCA 
TTCTCTCCAG TCCTGCTGCA GTTTTGTACA GATCCTAAAA ACCCCATTAC TGTCATCCGG 
GGACTAGCTG GCTCTCTCAG ATTAAATCTT GGTTTGTTTT CCACAAAGTC TTTGGTGGAG 
GCAAATTCAG AGCATGCAGT TGAGGTCCGA ACCCAGGTCC AGCAACCAGC AGATGAGAAC 
TGGGATGCCA GCGGCACCGG ACAGACCTGG CCCTGTGAGA GCAGCAGGTC ACATACAACA 
ATTGCCAAGT ATGCCCAGTA CCAGGCATCT AGCTTCCAAG AGAGCTTACA GGAAGAGAAA 
GGCAGTGACG CTGAGGAGGA TAATGATGAA AAGCCCACCA CTAAAGCGGA AAGTACAACC 
AGTAACTCTT CGGCACCCAA CTCCAGTTCA GAGCAGAAAA CTGTGGGGAA AATAATTAAA 
TTTGGCACAA ACATTGACCT GTCTGATCCC AAAAGATGGA AGCCTCAGCT GCAGGAACTG 
CAGAAGTTGC CAGCATTTAT GCGCGTATCC TCCAGTGGGA ATATGCTAAG CCATGTTGGC 
CACACCATTT TGGGAATGAA TACAGTACAA CTATATATGA AGATTCCTGG ATGCCGCACA 
CCAGGACACC AGGAGAACAA CAACTTCTGT TCTGTTAACA TCAACATTGG ACCTGGAGAC 
TGTGAGTGGT TTGCTGTCCA TGATGACTAC TGGGAGGCCA TCAGTGATTT TTGTCAGAAA 
CATGGAGTGG ACTATTTGAC GGGTTCATGG TGGCCAGTTC TGGATGACTT GTATCGCGCT 
AACATCCCTG TGTACCGTTT CATCCAAAGG CCAGGAGATC TGGTGTGGAT CAACGCAGGC 
ACTGTACACT GGGTTCAGGC CGTGGGCTGG TGCAACAACA TTGCCTGGAA TGTGGGACCC 
GTCAACGCTT ATCAGTACCA GGTGGCTTTA GAAAGATTTG AGTGGAACGA AGTGAAGAAA 
GTGAAATCCA TCGTTCCCAT GATTCATGTG TCCTGGAATG TGGCCCGCAC TGTTAAAGTC 
ACCGACCCAG ACGCTTACAA CATGATCAAA CACTGTCTCC TCCAGTCTAT TAAGCACATT 
CAGATTCTGA GGGATCAGCT GGTGGCAGCA GGAATGAAGA TTTCCTACCA GAGCAGAGTG 
AAGGATGAGC CGGCCTACTA CTGTAACGAG TGTGATGTGG AGGTGTTCAA CCTGCTGTTT 
GTGACTAGTG AGAACGGCAG TCGGAAGATG TACGTGGTGC ACTGTGAGGA CTGTGCACGT 
CAACGCAGCC CAAACCTCTC CAATGTAGTA GTGCTGGAGC AGTACCGCAT GGAGGAGCTC 
ATGAACATGT ACGACTCCTT CAGCCTGGCG GTCAGTCCCA GCTCTCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	KDM6BA
Entrez Gene ID	556678
Full Name	lysine (K)-specific demethylase 6B, a
General protein information	Preferred Names lysine (K)-specific demethylase 6B, a Names lysine-specific demethylase 6B
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

Danio rerio (zebrafish)	kdm6bb	NP_001025349.1
Danio rerio (zebrafish)	kdm6ba	XP_005172298.1

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KDM6BA cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

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