spef2 cDNA ORF clone, Danio rerio(zebrafish)

The following spef2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spef2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa41928 XM_009301185.3
Latest version!
Danio rerio sperm flagellar 2 (spef2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa33525 NM_001128232.1
Latest version!
Danio rerio sperm flagellar 2 (spef2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa41924 XM_009301181.3
Latest version!
Danio rerio sperm flagellar 2 (spef2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa41925 XM_009301182.3
Latest version!
Danio rerio sperm flagellar 2 (spef2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa41926 XM_009301183.3
Latest version!
Danio rerio sperm flagellar 2 (spef2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa41927 XM_009301184.3
Latest version!
Danio rerio sperm flagellar 2 (spef2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
ODa41925 XM_009301182.2 Danio rerio sperm flagellar 2 (spef2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa41926 XM_009301183.2 Danio rerio sperm flagellar 2 (spef2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa41928 XM_009301185.2 Danio rerio sperm flagellar 2 (spef2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa41924 XM_009301181.2 Danio rerio sperm flagellar 2 (spef2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa41927 XM_009301184.2 Danio rerio sperm flagellar 2 (spef2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa41928
    Clone ID Related Accession (Same CDS sequence) XM_009301185.2 , XM_009301185.3
    Accession Version XM_009301185.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3324bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product sperm flagellar protein 2 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301185.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAATGATC CCAAACCTAT CCCATATTTT AACTCCAACC ATATAAAGAG CTTGGAGAAA 
    AATGTTTCTC AGACAGAACA TACTACAGAA CAGCTTCAGA AAGATGAGCA GGAGACATCC
    TCTCCTCTAG AATCAGGACC ACAGCCTCAT GAGGCCTCAT CTGGGCTTCA TGAAAAAGAG
    GACAAAGAAG AATCAAAGGC AATCCCAAAG TGGTCATCAC GAGCACAGCA CGGTGCAGCG
    GTAGAGAAAG TTCTGAGGAC AGGCCAAGCT GTTCCTGATC AGCTTCTGGT GGACATTATT
    GTGGATGCTA TCAGGAACAT TCCTGCAGAT AGTGGCTGGA TTCTTGATGG CTTCCCGGTG
    GACATCAGTC AGGCTCAGAT GCTTGAGAAA GCTCTAAACA TGACTGAACT TGATGGAACA
    AAAACACAAA ATAATTTGTC CACAAACAAA AACGGCCCTA AAGATGCGGC TCCTCCATCC
    CCTGCTCTAG ACCTAGTGGT GCTGTTAGAC GTTTCAGATG AGCAGGTTCT GGAGCGAGCC
    ACAACTCAGC CTCCTGAGGG AACACAGATA GTGAATGGAG AAAGCATAAA GCAGGAGAAA
    GGCAGCAGTG ATGCTGCGGA GGAAAACACA AGCATGCCAG ATCTGAACAC ACAAGATCCT
    GAATCCTTGT CTGACGACAA AAGCCTGGGG AGGACACAAA TACAGTTCAG AATCAGTGGC
    TTTCATCAAA CATGGCCAAA ACTGGAGAAG TGGTTTGGTG ATCAGCAGAA TATTCTGGTG
    AAAGTCACAG CAGGTGTGGA CAAGGACACT CTCTTCAGTA ACGTGGAGAC TATTCTGAGG
    GACACTATTG AGTCTGTTGA AAAAGGAGCA GCTCTGGCTG ACTCCAGGAG AGCTTCAGTG
    ACCTCCACAC CTGAACAGAC CCGTCCAGAA AGTGCCTGCT CTTCCAAATC TTCCCGAGCT
    ACCCCAAAAT CTGCTAGAAG AAGCTTTGCG GAGGAACCTT TACCCAAGGA GTTGGCAGAA
    TATCTTGTGC CATACTGGGA GAACTTCTGT AGTTCATATG TGACTAATGT CAAAACAGTG
    ATGCAGAATC TCCGTGGTGA ACGTGAGCTC ATCATTCATC ACCTTTACAA CATCCGGGAG
    AACTTCAGGC AGTATCTACA GAAGCCGGAT CTGAAGCAGG AGTTTGTTAG CTCATGGCAG
    CGAGATTATA ACAGTGTTCC TGATAACATC AGAGAGGACG AGGAGACCAA AGGAGAGCTT
    CATCAAAGGC TGGATGACTT GCGTGAGCGG CTATGGGACA TCAGTGATAA GCGTAAAGAC
    GAGGCAGAGC AGGAGAAAGC AGGCGTCATT CATGATGGCT GGTTAGAGGA TCACACCGCT
    GTACTAATCA ACAATTATTC TGCATTGATG CAGATAGAAG TGGGCCGATT TCAGGACTGT
    GTGTGTCTGC TGAAAGATTA TTATTCAACG ATGGGTAAAA CAGCCTTTGA ATCCAAATGT
    GACTTTGCTC GTGTCCCATT GCTTGACATC ACCGCTGATC AGCATGTCGA CGAGTCGAAA
    ATCTCTGAAT CTGCTCCGTC AGAGAAATCT GTAAAGAATG CTGAGGAAAA AGACTCTGAA
    ATTGTGGACA GGATGAAAAC TAAAGTGTTT CCACTGGTTT CCTGCAGATC TCCAACTTCT
    GAGATCCTCA AACAGCCTTT GCATCAGGCT GATGAGAACC TGCTGCAGGA GTTCTTCCAA
    GCAGCAATCG CCATCTTCAG GAGCATGGTG TCGGCAGAGA CGCGGCAGTG GGATGAAGAA
    GAAGTTGATG AAGACCAGAA GCAGATGGAG GCACAGAAAG CTCAAACTTT GACCTCTGCT
    ACAGACGTAA GGAAAGCAGG AAAACAAAAA GGTGCTCCAC CTCCTCCTCC TCCAGAGCCC
    AGTCCTCAAC CTGCTGCAAG GAACCCAGAG GAGCTCAGGA AGAGAGCGGA GAAGAAACGA
    TTCAAACAGG AGCTCTCAGC TGCACTTAAA CCTGAAGAAA ATGCAGTGAA GCAGCGTTTG
    GAGCTAGTGA AGGCTCATGG GCTGAGAACA GTCATCAGTC TGCAGCACAA AGCAGAACAG
    GTCTATAGGA ACATGGAGGA GTGGATGAAA GCCCGCTATC TGGCTGAAAT GAAAAGTATC
    GACCAGCTGA CAGATTTGGT GAGGCAGCAC ATTGAGACTG GAGTTCAGAT CCGTCATGAG
    CTGGTTCTGC AGAGCACTGA TTTCTGTGTG GATGGAGACA CCCGTGTGCT GTTGAGTCCA
    GAGCTTACTC CGCGCCGATC CCTGCTGGAG CAGCCTAAAA ACAGCACACT CACAGTACAA
    CAGCTGCACG TCCTCTGCAC ACAGTTGCGC AAGATCGCCC CTAAAGGTCT GATGTGTAGA
    AGTGAGCTGA CTGAAGCTCT ACACGAATTA ACCTCTGCTC ACATGGGTAC TGATGTCCTT
    CCTGAGGCCT GGATGCACAT CACTCCTTCA CAGGTTGAGG AGCTGGTGTG TATTCTAGCT
    CAGGACTCTG AGATGTTGGA CTGGCGTCAG TTTCTGCTCA GTGCTGCTCT GCCGTGGCCA
    TTTCCCACTC AGACTCAGCT GATAAAAACC CTCCAGCGCT ACAGAGACAT CGACACCGCG
    GAAACCGGAC TCATCACACA AGAACAATAT GAACAGGTTG AATTATGGTT TCCCAGTGAA
    CAAGATCCTT CTGTCCCTGA TGACCCAACT GAACCCGTGC CCTATGACAG ATTGGCCAAC
    CTTAAAAAGT TTTTCTTCAG TCTGTTTGCG AGCACACACT CCTCACCACT GCTGCTGGAC
    TATCTGATGA TGCTGCTTCA TTTCTGCTGC CATCCTGAAG CAGCTCAGGG CTTCACCAGA
    GCGCTCGGCC TCGTCACCGG ACACACACTG CACTACAAAC ACACCACCCC ACTCACACAG
    TCTGTGCCGT GTCTGGAGGG CGCTGGAGTT GTGGATGATG CTGGTGCTGA TAATGAAGAG
    GAGCAGCACA GAGGGGTGGA GTCTGAGGAA GTGGGCGTGT CTGTTGATGA TGTGCTCAGA
    GTTTTGAGTC ATGGAGAGGA TCTCGTCTTA CATCTAAACC GCTTCCAGCT TGTCCACAGG
    AGCAGAGATG AACTCCGAGA GGAGCTTCTG AAGGTGTTTA AGGACTTGGG TTTCAGTGGT
    GAAGAGAAGA TTCCTTTCTC CACACTATCT CAGCATCCCT TTCTGCAGGA CCTGATAGAA
    GGATCCGCCT ATTATCTGCT CCATGACATT CACAAAATTT TCCAGACAAA ACAAACTGAA
    GATTCGAGAA GTTTCTCAAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299460.1
    CDS38..3361
    Translation

    Target ORF information:

    RefSeq Version XM_009301185.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sperm flagellar 2 (spef2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301185.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAATGATC CCAAACCTAT CCCATATTTT AACTCCAACC ATATAAAGAG CTTGGAGAAA 
    AATGTTTCTC AGACAGAACA TACTACAGAA CAGCTTCAGA AAGATGAGCA GGAGACATCC
    TCTCCTCTAG AATCAGGACC ACAGCCTCAT GAGGCCTCAT CTGGGCTTCA TGAAAAAGAG
    GACAAAGAAG AATCAAAGGC AATCCCAAAG TGGTCATCAC GAGCACAGCA CGGTGCAGCG
    GTAGAGAAAG TTCTGAGGAC AGGCCAAGCT GTTCCTGATC AGCTTCTGGT GGACATTATT
    GTGGATGCTA TCAGGAACAT TCCTGCAGAT AGTGGCTGGA TTCTTGATGG CTTCCCGGTG
    GACATCAGTC AGGCTCAGAT GCTTGAGAAA GCTCTAAACA TGACTGAACT TGATGGAACA
    AAAACACAAA ATAATTTGTC CACAAACAAA AACGGCCCTA AAGATGCGGC TCCTCCATCC
    CCTGCTCTAG ACCTAGTGGT GCTGTTAGAC GTTTCAGATG AGCAGGTTCT GGAGCGAGCC
    ACAACTCAGC CTCCTGAGGG AACACAGATA GTGAATGGAG AAAGCATAAA GCAGGAGAAA
    GGCAGCAGTG ATGCTGCGGA GGAAAACACA AGCATGCCAG ATCTGAACAC ACAAGATCCT
    GAATCCTTGT CTGACGACAA AAGCCTGGGG AGGACACAAA TACAGTTCAG AATCAGTGGC
    TTTCATCAAA CATGGCCAAA ACTGGAGAAG TGGTTTGGTG ATCAGCAGAA TATTCTGGTG
    AAAGTCACAG CAGGTGTGGA CAAGGACACT CTCTTCAGTA ACGTGGAGAC TATTCTGAGG
    GACACTATTG AGTCTGTTGA AAAAGGAGCA GCTCTGGCTG ACTCCAGGAG AGCTTCAGTG
    ACCTCCACAC CTGAACAGAC CCGTCCAGAA AGTGCCTGCT CTTCCAAATC TTCCCGAGCT
    ACCCCAAAAT CTGCTAGAAG AAGCTTTGCG GAGGAACCTT TACCCAAGGA GTTGGCAGAA
    TATCTTGTGC CATACTGGGA GAACTTCTGT AGTTCATATG TGACTAATGT CAAAACAGTG
    ATGCAGAATC TCCGTGGTGA ACGTGAGCTC ATCATTCATC ACCTTTACAA CATCCGGGAG
    AACTTCAGGC AGTATCTACA GAAGCCGGAT CTGAAGCAGG AGTTTGTTAG CTCATGGCAG
    CGAGATTATA ACAGTGTTCC TGATAACATC AGAGAGGACG AGGAGACCAA AGGAGAGCTT
    CATCAAAGGC TGGATGACTT GCGTGAGCGG CTATGGGACA TCAGTGATAA GCGTAAAGAC
    GAGGCAGAGC AGGAGAAAGC AGGCGTCATT CATGATGGCT GGTTAGAGGA TCACACCGCT
    GTACTAATCA ACAATTATTC TGCATTGATG CAGATAGAAG TGGGCCGATT TCAGGACTGT
    GTGTGTCTGC TGAAAGATTA TTATTCAACG ATGGGTAAAA CAGCCTTTGA ATCCAAATGT
    GACTTTGCTC GTGTCCCATT GCTTGACATC ACCGCTGATC AGCATGTCGA CGAGTCGAAA
    ATCTCTGAAT CTGCTCCGTC AGAGAAATCT GTAAAGAATG CTGAGGAAAA AGACTCTGAA
    ATTGTGGACA GGATGAAAAC TAAAGTGTTT CCACTGGTTT CCTGCAGATC TCCAACTTCT
    GAGATCCTCA AACAGCCTTT GCATCAGGCT GATGAGAACC TGCTGCAGGA GTTCTTCCAA
    GCAGCAATCG CCATCTTCAG GAGCATGGTG TCGGCAGAGA CGCGGCAGTG GGATGAAGAA
    GAAGTTGATG AAGACCAGAA GCAGATGGAG GCACAGAAAG CTCAAACTTT GACCTCTGCT
    ACAGACGTAA GGAAAGCAGG AAAACAAAAA GGTGCTCCAC CTCCTCCTCC TCCAGAGCCC
    AGTCCTCAAC CTGCTGCAAG GAACCCAGAG GAGCTCAGGA AGAGAGCGGA GAAGAAACGA
    TTCAAACAGG AGCTCTCAGC TGCACTTAAA CCTGAAGAAA ATGCAGTGAA GCAGCGTTTG
    GAGCTAGTGA AGGCTCATGG GCTGAGAACA GTCATCAGTC TGCAGCACAA AGCAGAACAG
    GTCTATAGGA ACATGGAGGA GTGGATGAAA GCCCGCTATC TGGCTGAAAT GAAAAGTATC
    GACCAGCTGA CAGATTTGGT GAGGCAGCAC ATTGAGACTG GAGTTCAGAT CCGTCATGAG
    CTGGTTCTGC AGAGCACTGA TTTCTGTGTG GATGGAGACA CCCGTGTGCT GTTGAGTCCA
    GAGCTTACTC CGCGCCGATC CCTGCTGGAG CAGCCTAAAA ACAGCACACT CACAGTACAA
    CAGCTGCACG TCCTCTGCAC ACAGTTGCGC AAGATCGCCC CTAAAGGTCT GATGTGTAGA
    AGTGAGCTGA CTGAAGCTCT ACACGAATTA ACCTCTGCTC ACATGGGTAC TGATGTCCTT
    CCTGAGGCCT GGATGCACAT CACTCCTTCA CAGGTTGAGG AGCTGGTGTG TATTCTAGCT
    CAGGACTCTG AGATGTTGGA CTGGCGTCAG TTTCTGCTCA GTGCTGCTCT GCCGTGGCCA
    TTTCCCACTC AGACTCAGCT GATAAAAACC CTCCAGCGCT ACAGAGACAT CGACACCGCG
    GAAACCGGAC TCATCACACA AGAACAATAT GAACAGGTTG AATTATGGTT TCCCAGTGAA
    CAAGATCCTT CTGTCCCTGA TGACCCAACT GAACCCGTGC CCTATGACAG ATTGGCCAAC
    CTTAAAAAGT TTTTCTTCAG TCTGTTTGCG AGCACACACT CCTCACCACT GCTGCTGGAC
    TATCTGATGA TGCTGCTTCA TTTCTGCTGC CATCCTGAAG CAGCTCAGGG CTTCACCAGA
    GCGCTCGGCC TCGTCACCGG ACACACACTG CACTACAAAC ACACCACCCC ACTCACACAG
    TCTGTGCCGT GTCTGGAGGG CGCTGGAGTT GTGGATGATG CTGGTGCTGA TAATGAAGAG
    GAGCAGCACA GAGGGGTGGA GTCTGAGGAA GTGGGCGTGT CTGTTGATGA TGTGCTCAGA
    GTTTTGAGTC ATGGAGAGGA TCTCGTCTTA CATCTAAACC GCTTCCAGCT TGTCCACAGG
    AGCAGAGATG AACTCCGAGA GGAGCTTCTG AAGGTGTTTA AGGACTTGGG TTTCAGTGGT
    GAAGAGAAGA TTCCTTTCTC CACACTATCT CAGCATCCCT TTCTGCAGGA CCTGATAGAA
    GGATCCGCCT ATTATCTGCT CCATGACATT CACAAAATTT TCCAGACAAA ACAAACTGAA
    GATTCGAGAA GTTTCTCAAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41928
    Clone ID Related Accession (Same CDS sequence) XM_009301185.2 , XM_009301185.3
    Accession Version XM_009301185.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3324bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product sperm flagellar protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301185.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAATGATC CCAAACCTAT CCCATATTTT AACTCCAACC ATATAAAGAG CTTGGAGAAA 
    AATGTTTCTC AGACAGAACA TACTACAGAA CAGCTTCAGA AAGATGAGCA GGAGACATCC
    TCTCCTCTAG AATCAGGACC ACAGCCTCAT GAGGCCTCAT CTGGGCTTCA TGAAAAAGAG
    GACAAAGAAG AATCAAAGGC AATCCCAAAG TGGTCATCAC GAGCACAGCA CGGTGCAGCG
    GTAGAGAAAG TTCTGAGGAC AGGCCAAGCT GTTCCTGATC AGCTTCTGGT GGACATTATT
    GTGGATGCTA TCAGGAACAT TCCTGCAGAT AGTGGCTGGA TTCTTGATGG CTTCCCGGTG
    GACATCAGTC AGGCTCAGAT GCTTGAGAAA GCTCTAAACA TGACTGAACT TGATGGAACA
    AAAACACAAA ATAATTTGTC CACAAACAAA AACGGCCCTA AAGATGCGGC TCCTCCATCC
    CCTGCTCTAG ACCTAGTGGT GCTGTTAGAC GTTTCAGATG AGCAGGTTCT GGAGCGAGCC
    ACAACTCAGC CTCCTGAGGG AACACAGATA GTGAATGGAG AAAGCATAAA GCAGGAGAAA
    GGCAGCAGTG ATGCTGCGGA GGAAAACACA AGCATGCCAG ATCTGAACAC ACAAGATCCT
    GAATCCTTGT CTGACGACAA AAGCCTGGGG AGGACACAAA TACAGTTCAG AATCAGTGGC
    TTTCATCAAA CATGGCCAAA ACTGGAGAAG TGGTTTGGTG ATCAGCAGAA TATTCTGGTG
    AAAGTCACAG CAGGTGTGGA CAAGGACACT CTCTTCAGTA ACGTGGAGAC TATTCTGAGG
    GACACTATTG AGTCTGTTGA AAAAGGAGCA GCTCTGGCTG ACTCCAGGAG AGCTTCAGTG
    ACCTCCACAC CTGAACAGAC CCGTCCAGAA AGTGCCTGCT CTTCCAAATC TTCCCGAGCT
    ACCCCAAAAT CTGCTAGAAG AAGCTTTGCG GAGGAACCTT TACCCAAGGA GTTGGCAGAA
    TATCTTGTGC CATACTGGGA GAACTTCTGT AGTTCATATG TGACTAATGT CAAAACAGTG
    ATGCAGAATC TCCGTGGTGA ACGTGAGCTC ATCATTCATC ACCTTTACAA CATCCGGGAG
    AACTTCAGGC AGTATCTACA GAAGCCGGAT CTGAAGCAGG AGTTTGTTAG CTCATGGCAG
    CGAGATTATA ACAGTGTTCC TGATAACATC AGAGAGGACG AGGAGACCAA AGGAGAGCTT
    CATCAAAGGC TGGATGACTT GCGTGAGCGG CTATGGGACA TCAGTGATAA GCGTAAAGAC
    GAGGCAGAGC AGGAGAAAGC AGGCGTCATT CATGATGGCT GGTTAGAGGA TCACACCGCT
    GTACTAATCA ACAATTATTC TGCATTGATG CAGATAGAAG TGGGCCGATT TCAGGACTGT
    GTGTGTCTGC TGAAAGATTA TTATTCAACG ATGGGTAAAA CAGCCTTTGA ATCCAAATGT
    GACTTTGCTC GTGTCCCATT GCTTGACATC ACCGCTGATC AGCATGTCGA CGAGTCGAAA
    ATCTCTGAAT CTGCTCCGTC AGAGAAATCT GTAAAGAATG CTGAGGAAAA AGACTCTGAA
    ATTGTGGACA GGATGAAAAC TAAAGTGTTT CCACTGGTTT CCTGCAGATC TCCAACTTCT
    GAGATCCTCA AACAGCCTTT GCATCAGGCT GATGAGAACC TGCTGCAGGA GTTCTTCCAA
    GCAGCAATCG CCATCTTCAG GAGCATGGTG TCGGCAGAGA CGCGGCAGTG GGATGAAGAA
    GAAGTTGATG AAGACCAGAA GCAGATGGAG GCACAGAAAG CTCAAACTTT GACCTCTGCT
    ACAGACGTAA GGAAAGCAGG AAAACAAAAA GGTGCTCCAC CTCCTCCTCC TCCAGAGCCC
    AGTCCTCAAC CTGCTGCAAG GAACCCAGAG GAGCTCAGGA AGAGAGCGGA GAAGAAACGA
    TTCAAACAGG AGCTCTCAGC TGCACTTAAA CCTGAAGAAA ATGCAGTGAA GCAGCGTTTG
    GAGCTAGTGA AGGCTCATGG GCTGAGAACA GTCATCAGTC TGCAGCACAA AGCAGAACAG
    GTCTATAGGA ACATGGAGGA GTGGATGAAA GCCCGCTATC TGGCTGAAAT GAAAAGTATC
    GACCAGCTGA CAGATTTGGT GAGGCAGCAC ATTGAGACTG GAGTTCAGAT CCGTCATGAG
    CTGGTTCTGC AGAGCACTGA TTTCTGTGTG GATGGAGACA CCCGTGTGCT GTTGAGTCCA
    GAGCTTACTC CGCGCCGATC CCTGCTGGAG CAGCCTAAAA ACAGCACACT CACAGTACAA
    CAGCTGCACG TCCTCTGCAC ACAGTTGCGC AAGATCGCCC CTAAAGGTCT GATGTGTAGA
    AGTGAGCTGA CTGAAGCTCT ACACGAATTA ACCTCTGCTC ACATGGGTAC TGATGTCCTT
    CCTGAGGCCT GGATGCACAT CACTCCTTCA CAGGTTGAGG AGCTGGTGTG TATTCTAGCT
    CAGGACTCTG AGATGTTGGA CTGGCGTCAG TTTCTGCTCA GTGCTGCTCT GCCGTGGCCA
    TTTCCCACTC AGACTCAGCT GATAAAAACC CTCCAGCGCT ACAGAGACAT CGACACCGCG
    GAAACCGGAC TCATCACACA AGAACAATAT GAACAGGTTG AATTATGGTT TCCCAGTGAA
    CAAGATCCTT CTGTCCCTGA TGACCCAACT GAACCCGTGC CCTATGACAG ATTGGCCAAC
    CTTAAAAAGT TTTTCTTCAG TCTGTTTGCG AGCACACACT CCTCACCACT GCTGCTGGAC
    TATCTGATGA TGCTGCTTCA TTTCTGCTGC CATCCTGAAG CAGCTCAGGG CTTCACCAGA
    GCGCTCGGCC TCGTCACCGG ACACACACTG CACTACAAAC ACACCACCCC ACTCACACAG
    TCTGTGCCGT GTCTGGAGGG CGCTGGAGTT GTGGATGATG CTGGTGCTGA TAATGAAGAG
    GAGCAGCACA GAGGGGTGGA GTCTGAGGAA GTGGGCGTGT CTGTTGATGA TGTGCTCAGA
    GTTTTGAGTC ATGGAGAGGA TCTCGTCTTA CATCTAAACC GCTTCCAGCT TGTCCACAGG
    AGCAGAGATG AACTCCGAGA GGAGCTTCTG AAGGTGTTTA AGGACTTGGG TTTCAGTGGT
    GAAGAGAAGA TTCCTTTCTC CACACTATCT CAGCATCCCT TTCTGCAGGA CCTGATAGAA
    GGATCCGCCT ATTATCTGCT CCATGACATT CACAAAATTT TCCAGACAAA ACAAACTGAA
    GATTCGAGAA GTTTCTCAAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299460.1
    CDS36..3359
    Translation

    Target ORF information:

    RefSeq Version XM_009301185.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sperm flagellar 2 (spef2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301185.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAATGATC CCAAACCTAT CCCATATTTT AACTCCAACC ATATAAAGAG CTTGGAGAAA 
    AATGTTTCTC AGACAGAACA TACTACAGAA CAGCTTCAGA AAGATGAGCA GGAGACATCC
    TCTCCTCTAG AATCAGGACC ACAGCCTCAT GAGGCCTCAT CTGGGCTTCA TGAAAAAGAG
    GACAAAGAAG AATCAAAGGC AATCCCAAAG TGGTCATCAC GAGCACAGCA CGGTGCAGCG
    GTAGAGAAAG TTCTGAGGAC AGGCCAAGCT GTTCCTGATC AGCTTCTGGT GGACATTATT
    GTGGATGCTA TCAGGAACAT TCCTGCAGAT AGTGGCTGGA TTCTTGATGG CTTCCCGGTG
    GACATCAGTC AGGCTCAGAT GCTTGAGAAA GCTCTAAACA TGACTGAACT TGATGGAACA
    AAAACACAAA ATAATTTGTC CACAAACAAA AACGGCCCTA AAGATGCGGC TCCTCCATCC
    CCTGCTCTAG ACCTAGTGGT GCTGTTAGAC GTTTCAGATG AGCAGGTTCT GGAGCGAGCC
    ACAACTCAGC CTCCTGAGGG AACACAGATA GTGAATGGAG AAAGCATAAA GCAGGAGAAA
    GGCAGCAGTG ATGCTGCGGA GGAAAACACA AGCATGCCAG ATCTGAACAC ACAAGATCCT
    GAATCCTTGT CTGACGACAA AAGCCTGGGG AGGACACAAA TACAGTTCAG AATCAGTGGC
    TTTCATCAAA CATGGCCAAA ACTGGAGAAG TGGTTTGGTG ATCAGCAGAA TATTCTGGTG
    AAAGTCACAG CAGGTGTGGA CAAGGACACT CTCTTCAGTA ACGTGGAGAC TATTCTGAGG
    GACACTATTG AGTCTGTTGA AAAAGGAGCA GCTCTGGCTG ACTCCAGGAG AGCTTCAGTG
    ACCTCCACAC CTGAACAGAC CCGTCCAGAA AGTGCCTGCT CTTCCAAATC TTCCCGAGCT
    ACCCCAAAAT CTGCTAGAAG AAGCTTTGCG GAGGAACCTT TACCCAAGGA GTTGGCAGAA
    TATCTTGTGC CATACTGGGA GAACTTCTGT AGTTCATATG TGACTAATGT CAAAACAGTG
    ATGCAGAATC TCCGTGGTGA ACGTGAGCTC ATCATTCATC ACCTTTACAA CATCCGGGAG
    AACTTCAGGC AGTATCTACA GAAGCCGGAT CTGAAGCAGG AGTTTGTTAG CTCATGGCAG
    CGAGATTATA ACAGTGTTCC TGATAACATC AGAGAGGACG AGGAGACCAA AGGAGAGCTT
    CATCAAAGGC TGGATGACTT GCGTGAGCGG CTATGGGACA TCAGTGATAA GCGTAAAGAC
    GAGGCAGAGC AGGAGAAAGC AGGCGTCATT CATGATGGCT GGTTAGAGGA TCACACCGCT
    GTACTAATCA ACAATTATTC TGCATTGATG CAGATAGAAG TGGGCCGATT TCAGGACTGT
    GTGTGTCTGC TGAAAGATTA TTATTCAACG ATGGGTAAAA CAGCCTTTGA ATCCAAATGT
    GACTTTGCTC GTGTCCCATT GCTTGACATC ACCGCTGATC AGCATGTCGA CGAGTCGAAA
    ATCTCTGAAT CTGCTCCGTC AGAGAAATCT GTAAAGAATG CTGAGGAAAA AGACTCTGAA
    ATTGTGGACA GGATGAAAAC TAAAGTGTTT CCACTGGTTT CCTGCAGATC TCCAACTTCT
    GAGATCCTCA AACAGCCTTT GCATCAGGCT GATGAGAACC TGCTGCAGGA GTTCTTCCAA
    GCAGCAATCG CCATCTTCAG GAGCATGGTG TCGGCAGAGA CGCGGCAGTG GGATGAAGAA
    GAAGTTGATG AAGACCAGAA GCAGATGGAG GCACAGAAAG CTCAAACTTT GACCTCTGCT
    ACAGACGTAA GGAAAGCAGG AAAACAAAAA GGTGCTCCAC CTCCTCCTCC TCCAGAGCCC
    AGTCCTCAAC CTGCTGCAAG GAACCCAGAG GAGCTCAGGA AGAGAGCGGA GAAGAAACGA
    TTCAAACAGG AGCTCTCAGC TGCACTTAAA CCTGAAGAAA ATGCAGTGAA GCAGCGTTTG
    GAGCTAGTGA AGGCTCATGG GCTGAGAACA GTCATCAGTC TGCAGCACAA AGCAGAACAG
    GTCTATAGGA ACATGGAGGA GTGGATGAAA GCCCGCTATC TGGCTGAAAT GAAAAGTATC
    GACCAGCTGA CAGATTTGGT GAGGCAGCAC ATTGAGACTG GAGTTCAGAT CCGTCATGAG
    CTGGTTCTGC AGAGCACTGA TTTCTGTGTG GATGGAGACA CCCGTGTGCT GTTGAGTCCA
    GAGCTTACTC CGCGCCGATC CCTGCTGGAG CAGCCTAAAA ACAGCACACT CACAGTACAA
    CAGCTGCACG TCCTCTGCAC ACAGTTGCGC AAGATCGCCC CTAAAGGTCT GATGTGTAGA
    AGTGAGCTGA CTGAAGCTCT ACACGAATTA ACCTCTGCTC ACATGGGTAC TGATGTCCTT
    CCTGAGGCCT GGATGCACAT CACTCCTTCA CAGGTTGAGG AGCTGGTGTG TATTCTAGCT
    CAGGACTCTG AGATGTTGGA CTGGCGTCAG TTTCTGCTCA GTGCTGCTCT GCCGTGGCCA
    TTTCCCACTC AGACTCAGCT GATAAAAACC CTCCAGCGCT ACAGAGACAT CGACACCGCG
    GAAACCGGAC TCATCACACA AGAACAATAT GAACAGGTTG AATTATGGTT TCCCAGTGAA
    CAAGATCCTT CTGTCCCTGA TGACCCAACT GAACCCGTGC CCTATGACAG ATTGGCCAAC
    CTTAAAAAGT TTTTCTTCAG TCTGTTTGCG AGCACACACT CCTCACCACT GCTGCTGGAC
    TATCTGATGA TGCTGCTTCA TTTCTGCTGC CATCCTGAAG CAGCTCAGGG CTTCACCAGA
    GCGCTCGGCC TCGTCACCGG ACACACACTG CACTACAAAC ACACCACCCC ACTCACACAG
    TCTGTGCCGT GTCTGGAGGG CGCTGGAGTT GTGGATGATG CTGGTGCTGA TAATGAAGAG
    GAGCAGCACA GAGGGGTGGA GTCTGAGGAA GTGGGCGTGT CTGTTGATGA TGTGCTCAGA
    GTTTTGAGTC ATGGAGAGGA TCTCGTCTTA CATCTAAACC GCTTCCAGCT TGTCCACAGG
    AGCAGAGATG AACTCCGAGA GGAGCTTCTG AAGGTGTTTA AGGACTTGGG TTTCAGTGGT
    GAAGAGAAGA TTCCTTTCTC CACACTATCT CAGCATCCCT TTCTGCAGGA CCTGATAGAA
    GGATCCGCCT ATTATCTGCT CCATGACATT CACAAAATTT TCCAGACAAA ACAAACTGAA
    GATTCGAGAA GTTTCTCAAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa33525
    Clone ID Related Accession (Same CDS sequence) NM_001128232.1
    Accession Version NM_001128232.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4920bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-07-05
    Organism Danio rerio(zebrafish)
    Product sperm flagellar protein 2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR450726.6. On Jul 31, 2008 this sequence version replaced XM_694196.3. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCAGACA TTATCTGTCA GTGGCTGAAT TCAGAGCTCC GGCTGTCAAA AGTCGTCGAG 
    CCGCATTCAT TTGCCAGGGA TTTTTCAAAC GGTTATCTGA TAGGAGAGAT CCTCTACAGA
    TATGAGCTAC AAGACGATTT CCATCTGTTC TCAAAACAAA ACACATCGAA TGCCAAGCTA
    AATAACTTCA CTCGCATTGA GCCCACCCTG CAGCTGCTGA CCGTGCCCTT TGATCTGACC
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA AGAGCTTGGA GAAAAATGTT
    TCTCAGACAG AACATACTAC AGAACAGCTT CAGAAAGATG AGCAGGAGAC ATCCTCTCCT
    CTAGAATCAG GACCACAGCC TCATGAGGCC TCATCTGGGC TTCATGAAAA AGAGGACAAA
    GAAGAATCAA AGGCAATCCC AAAGTGGTCA TCACGAGCAC AGCACGGTGC AGCGGTAGAG
    AAAGTTCTGA GGACAGGCCA AGCTGTTCCT GATCAGCTTC TGGTGGACAT TATTGTGGAT
    GCTATCAGGA ACATTCCTGC AGATAGTGGC TGGATTCTTG ATGGCTTCCC GGTGGACATC
    AGTCAGGCTC AGATGCTTGA GAAAGCTCTA AACATGACTG AACTTGATGG AACAAAAACA
    CAAAATAATT TGTCCACAAA CAAAAACGGC CCTAAAGATG CGGCTCCTCC ATCCCCTGCT
    CTAGACCTAG TGGTGCTGTT AGACGTTTCA GATGAGCAGG TTCTGGAGCG AGCCACAACT
    CAGCCTCCTG AGGGAACACA GATAGTGAAT GGAGAAAGCA TAAAGCAGGA GAAAGGCAGC
    AGTGATGCTG CGGAGGAAAA CACAAGCATG CCAGATCTGA ACACACAAGA TCCTGAATCC
    TTGTCTGACG ACAAAAGCCT GGGGAGGACA CAAATACAGT TCAGAATCAG TGGCTTTCAT
    CAAACATGGC CAAAACTGGA GAAGTGGTTT GGTGATCAGC AGAATATTCT GGTGAAAGTC
    ACAGCAGGTG TGGACAAGGA CACTCTCTTC AGTAACGTGG AGACTATTCT GAGGGACACT
    ATTGAGTCTG TTGAAAAAGG AGCAGCTCTG GCTGACTCCA GGAGAGCTTC AGTGACCTCC
    ACACCTGAAC AGACCCGTCC AGAAATGATG CAGAATCTCC GTGGTGAACG TGAGCTCATC
    ATTCATCACC TTTACAACAT CCGGGAGAAC TTCAGGCAGT ATCTACAGAA GCCGGATCTG
    AAGCAGGAGT TTGTTAGCTC ATGGCAGCGA GATTATAACA GTGTTCCTGA TAACATCAGA
    GAGGACGAGG AGACCAAAGG AGAGCTTCAT CAAAGGCTGG ATGACTTGCG TGAGCGGCTA
    TGGGACATCA GTGATAAGCG TAAAGACGAG GCAGAGCAGG AGAAAGCAGG CGTCATTCAT
    GATGGCTGGT TAGAGGATCA CACCGCTGTA CTAATCAACA ATTATTCTGC ATTGATGCAG
    ATAGAAGTGG GCCGATTTCA GGACTGTGTG TGTCTGCTGA AAGATTATTA TTCAACGATG
    GGTAAAACAG CCTTTGAATC CAAATGTGAC TTTGCTCGTG TCCCATTGCT TGACATCACC
    GCTGATCAGC ATGTCGACGA GTCGAAAATC TCTGAATCTG CTCCGTCAGA GAAATCTGTA
    AAGAATGCTG AGGAAAAAGA CTCTGAAATT GTGGACAGGA TGAAAACTAA AGTATCTCCA
    ACTTCTGAGA TCCTCAAACA GCCTTTGCAT CAGGCTGATG AGAACCTGCT GCAGGAGTTC
    TTCCAAGCAG CAATCGCCAT CTTCAGGAGC ATGGTGTCGG CAGAGACGCG GCAGTGGGAT
    GAAGAAGAAG TTGATGAAGA CCAGAAGCAG ATGGAGGCAC AGAAAGCTCA AACTTTGACC
    TCTGCTACAG ACGTAAGGAA AGCAGGAAAA CAAAAAGGTG CTCCACCTCC TCCTCCTCCA
    GAGCCCAGTC CTCAACCTGC TGCAAGGAAC CCAGAGGAGC TCAGGAAGAG AGCGGAGAAG
    AAACGATTCA AACAGGAGCT CTCAGCTGCA CTTAAACCTG AAGTGAAGGC TCATGGGCTG
    AGAACAGTCA TCAGTCTGCA GCACAAAGCA GAACAGGTCT ATAGGAACAT GGAGGAGTGG
    ATGAAAGCCC GCTATCTGGC TGAAATGAAA AGTATCGACC AGCTGACAGA TTTGGTGAGG
    CAGCACATTG AGACTGGAGT TCAGATCCGT CATGAGCTGG TTCTGCAGAG CACTGATTTC
    TGTGTGGATG GAGACACCCG TGTGCTGTTG AGTCCAGAGC TTACTCCGCG CCGATCCCTG
    CTGGAGCAGC CTAAAAACAG CACACTCACA GTACAACAGC TGCACGTCCT CTGCACACAG
    TTGCGCAAGA TCGCCCCTAA AGGTCTGATG TGTAGAAGTG AGCTGACTGA AGCTCTACAC
    GAATTAACCT CTGCTCACAT GGGTACTGAT GTCCTTCCTG AGGCCTGGAT GCACATCACT
    CCTTCACAGG TTGAGGAGCT GGTGTGTATT CTAGCTCAGG ACTCTGAGAT GTTGGACTGG
    CGTCAGTTTC TGCTCAGTGC TGCTCTGCCG TGGCCATTTC CCACTCAGAC TCAGCTGATA
    AAAACCCTCC AGCGCTACAG AGACATCGAC ACCGCGGAAA CCGGACTCAT CACACAAGAA
    CAATATGAAC AGGTTGAATT ATGGTTTCCC AGTGAACAAG ATCCTTCTGT CCCTGATGAC
    CCAACTGAAC CCGTGCCCTA TGACAGATTG GCCAACCTTA AAAAGTTTTT CTTCAGTCTG
    TTTGCGAGCA CACACTCCTC ACCACTGCTG CTGGACTATC TGATGATGCT GCTTCATTTC
    TGCTGCCATC CTGAAGCAGC TCAGGGCTTC ACCAGAGCGC TCGGCCTCGT CACCGGACAC
    ACACTGCACT ACAAACACAC CACCCCACTC ACACAGTCTG TGCCGTGTCT GGAGGGCGCT
    GGAGTTGTGG ATGATGCTGG TGCTGATAAT GAAGAGGAGC AGCACAGAGG GGTGGAGTCT
    GAGGAAGTGG GCGTGTCTGT TGATGATGTG CTCAGAGTTT TGAGTCATGG AGAGGATCTC
    GTCTTACATC TAAACCGCTT CCAGCTTGTC CACAGGAGCA GAGATGAACT CCGAGAGGAG
    CTTCTGAAGG TGTTTAAGGA CTTGGGTTTC AGTGGTGAAG AGAAGATTCC TTTCTCCACA
    CTATCTCAGC ATCCCTTTCT GCAGGACCTG ATAGAAGGAT CCGCCTATTA TCTGCTCCAT
    GACATTCACA AAATTTTCCA GACAAAACAA ACTGAAGATT CGAGAAGTTT CTCAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001121704.1
    CDS112..5031
    Translation

    Target ORF information:

    RefSeq Version NM_001128232.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sperm flagellar 2 (spef2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001128232.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCAGACA TTATCTGTCA GTGGCTGAAT TCAGAGCTCC GGCTGTCAAA AGTCGTCGAG 
    CCGCATTCAT TTGCCAGGGA TTTTTCAAAC GGTTATCTGA TAGGAGAGAT CCTCTACAGA
    TATGAGCTAC AAGACGATTT CCATCTGTTC TCAAAACAAA ACACATCGAA TGCCAAGCTA
    AATAACTTCA CTCGCATTGA GCCCACCCTG CAGCTGCTGA CCGTGCCCTT TGATCTGACC
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA AGAGCTTGGA GAAAAATGTT
    TCTCAGACAG AACATACTAC AGAACAGCTT CAGAAAGATG AGCAGGAGAC ATCCTCTCCT
    CTAGAATCAG GACCACAGCC TCATGAGGCC TCATCTGGGC TTCATGAAAA AGAGGACAAA
    GAAGAATCAA AGGCAATCCC AAAGTGGTCA TCACGAGCAC AGCACGGTGC AGCGGTAGAG
    AAAGTTCTGA GGACAGGCCA AGCTGTTCCT GATCAGCTTC TGGTGGACAT TATTGTGGAT
    GCTATCAGGA ACATTCCTGC AGATAGTGGC TGGATTCTTG ATGGCTTCCC GGTGGACATC
    AGTCAGGCTC AGATGCTTGA GAAAGCTCTA AACATGACTG AACTTGATGG AACAAAAACA
    CAAAATAATT TGTCCACAAA CAAAAACGGC CCTAAAGATG CGGCTCCTCC ATCCCCTGCT
    CTAGACCTAG TGGTGCTGTT AGACGTTTCA GATGAGCAGG TTCTGGAGCG AGCCACAACT
    CAGCCTCCTG AGGGAACACA GATAGTGAAT GGAGAAAGCA TAAAGCAGGA GAAAGGCAGC
    AGTGATGCTG CGGAGGAAAA CACAAGCATG CCAGATCTGA ACACACAAGA TCCTGAATCC
    TTGTCTGACG ACAAAAGCCT GGGGAGGACA CAAATACAGT TCAGAATCAG TGGCTTTCAT
    CAAACATGGC CAAAACTGGA GAAGTGGTTT GGTGATCAGC AGAATATTCT GGTGAAAGTC
    ACAGCAGGTG TGGACAAGGA CACTCTCTTC AGTAACGTGG AGACTATTCT GAGGGACACT
    ATTGAGTCTG TTGAAAAAGG AGCAGCTCTG GCTGACTCCA GGAGAGCTTC AGTGACCTCC
    ACACCTGAAC AGACCCGTCC AGAAATGATG CAGAATCTCC GTGGTGAACG TGAGCTCATC
    ATTCATCACC TTTACAACAT CCGGGAGAAC TTCAGGCAGT ATCTACAGAA GCCGGATCTG
    AAGCAGGAGT TTGTTAGCTC ATGGCAGCGA GATTATAACA GTGTTCCTGA TAACATCAGA
    GAGGACGAGG AGACCAAAGG AGAGCTTCAT CAAAGGCTGG ATGACTTGCG TGAGCGGCTA
    TGGGACATCA GTGATAAGCG TAAAGACGAG GCAGAGCAGG AGAAAGCAGG CGTCATTCAT
    GATGGCTGGT TAGAGGATCA CACCGCTGTA CTAATCAACA ATTATTCTGC ATTGATGCAG
    ATAGAAGTGG GCCGATTTCA GGACTGTGTG TGTCTGCTGA AAGATTATTA TTCAACGATG
    GGTAAAACAG CCTTTGAATC CAAATGTGAC TTTGCTCGTG TCCCATTGCT TGACATCACC
    GCTGATCAGC ATGTCGACGA GTCGAAAATC TCTGAATCTG CTCCGTCAGA GAAATCTGTA
    AAGAATGCTG AGGAAAAAGA CTCTGAAATT GTGGACAGGA TGAAAACTAA AGTATCTCCA
    ACTTCTGAGA TCCTCAAACA GCCTTTGCAT CAGGCTGATG AGAACCTGCT GCAGGAGTTC
    TTCCAAGCAG CAATCGCCAT CTTCAGGAGC ATGGTGTCGG CAGAGACGCG GCAGTGGGAT
    GAAGAAGAAG TTGATGAAGA CCAGAAGCAG ATGGAGGCAC AGAAAGCTCA AACTTTGACC
    TCTGCTACAG ACGTAAGGAA AGCAGGAAAA CAAAAAGGTG CTCCACCTCC TCCTCCTCCA
    GAGCCCAGTC CTCAACCTGC TGCAAGGAAC CCAGAGGAGC TCAGGAAGAG AGCGGAGAAG
    AAACGATTCA AACAGGAGCT CTCAGCTGCA CTTAAACCTG AAGTGAAGGC TCATGGGCTG
    AGAACAGTCA TCAGTCTGCA GCACAAAGCA GAACAGGTCT ATAGGAACAT GGAGGAGTGG
    ATGAAAGCCC GCTATCTGGC TGAAATGAAA AGTATCGACC AGCTGACAGA TTTGGTGAGG
    CAGCACATTG AGACTGGAGT TCAGATCCGT CATGAGCTGG TTCTGCAGAG CACTGATTTC
    TGTGTGGATG GAGACACCCG TGTGCTGTTG AGTCCAGAGC TTACTCCGCG CCGATCCCTG
    CTGGAGCAGC CTAAAAACAG CACACTCACA GTACAACAGC TGCACGTCCT CTGCACACAG
    TTGCGCAAGA TCGCCCCTAA AGGTCTGATG TGTAGAAGTG AGCTGACTGA AGCTCTACAC
    GAATTAACCT CTGCTCACAT GGGTACTGAT GTCCTTCCTG AGGCCTGGAT GCACATCACT
    CCTTCACAGG TTGAGGAGCT GGTGTGTATT CTAGCTCAGG ACTCTGAGAT GTTGGACTGG
    CGTCAGTTTC TGCTCAGTGC TGCTCTGCCG TGGCCATTTC CCACTCAGAC TCAGCTGATA
    AAAACCCTCC AGCGCTACAG AGACATCGAC ACCGCGGAAA CCGGACTCAT CACACAAGAA
    CAATATGAAC AGGTTGAATT ATGGTTTCCC AGTGAACAAG ATCCTTCTGT CCCTGATGAC
    CCAACTGAAC CCGTGCCCTA TGACAGATTG GCCAACCTTA AAAAGTTTTT CTTCAGTCTG
    TTTGCGAGCA CACACTCCTC ACCACTGCTG CTGGACTATC TGATGATGCT GCTTCATTTC
    TGCTGCCATC CTGAAGCAGC TCAGGGCTTC ACCAGAGCGC TCGGCCTCGT CACCGGACAC
    ACACTGCACT ACAAACACAC CACCCCACTC ACACAGTCTG TGCCGTGTCT GGAGGGCGCT
    GGAGTTGTGG ATGATGCTGG TGCTGATAAT GAAGAGGAGC AGCACAGAGG GGTGGAGTCT
    GAGGAAGTGG GCGTGTCTGT TGATGATGTG CTCAGAGTTT TGAGTCATGG AGAGGATCTC
    GTCTTACATC TAAACCGCTT CCAGCTTGTC CACAGGAGCA GAGATGAACT CCGAGAGGAG
    CTTCTGAAGG TGTTTAAGGA CTTGGGTTTC AGTGGTGAAG AGAAGATTCC TTTCTCCACA
    CTATCTCAGC ATCCCTTTCT GCAGGACCTG ATAGAAGGAT CCGCCTATTA TCTGCTCCAT
    GACATTCACA AAATTTTCCA GACAAAACAA ACTGAAGATT CGAGAAGTTT CTCAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41924
    Clone ID Related Accession (Same CDS sequence) XM_009301181.3 , XM_009301181.2
    Accession Version XM_009301181.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5118bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product sperm flagellar protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301181.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGACA TTATCTGTCA GTGGCTGAAT TCAGAGCTCC GGCTGTCAAA AGTCGTCGAG 
    CCGCATTCAT TTGCCAGGGA TTTTTCAAAC GGTTATCTGA TAGGAGAGAT CCTCTACAGA
    TATGAGCTAC AAGACGATTT CCATCTGTTC TCAAAACAAA ACACATCGAA TGCCAAGCTA
    AATAACTTCA CTCGCATTGA GCCCACCCTG CAGCTGCTGA CCGTGCCCTT TGATCTGACC
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA AGAGCTTGGA GAAAAATGTT
    TCTCAGACAG AACATACTAC AGAACAGCTT CAGAAAGATG AGCAGGAGAC ATCCTCTCCT
    CTAGAATCAG GACCACAGCC TCATGAGGCC TCATCTGGGC TTCATGAAAA AGAGGACAAA
    GAAGAATCAA AGGCAATCCC AAAGTGGTCA TCACGAGCAC AGCACGGTGC AGCGGTAGAG
    AAAGTTCTGA GGACAGGCCA AGCTGTTCCT GATCAGCTTC TGGTGGACAT TATTGTGGAT
    GCTATCAGGA ACATTCCTGC AGATAGTGGC TGGATTCTTG ATGGCTTCCC GGTGGACATC
    AGTCAGGCTC AGATGCTTGA GAAAGCTCTA AACATGACTG AACTTGATGG AACAAAAACA
    CAAAATAATT TGTCCACAAA CAAAAACGGC CCTAAAGATG CGGCTCCTCC ATCCCCTGCT
    CTAGACCTAG TGGTGCTGTT AGACGTTTCA GATGAGCAGG TTCTGGAGCG AGCCACAACT
    CAGCCTCCTG AGGGAACACA GATAGTGAAT GGAGAAAGCA TAAAGCAGGA GAAAGGCAGC
    AGTGATGCTG CGGAGGAAAA CACAAGCATG CCAGATCTGA ACACACAAGA TCCTGAATCC
    TTGTCTGACG ACAAAAGCCT GGGGAGGACA CAAATACAGT TCAGAATCAG TGGCTTTCAT
    CAAACATGGC CAAAACTGGA GAAGTGGTTT GGTGATCAGC AGAATATTCT GGTGAAAGTC
    ACAGCAGGTG TGGACAAGGA CACTCTCTTC AGTAACGTGG AGACTATTCT GAGGGACACT
    ATTGAGTCTG TTGAAAAAGG AGCAGCTCTG GCTGACTCCA GGAGAGCTTC AGTGACCTCC
    ACACCTGAAC AGACCCGTCC AGAAAGTGCC TGCTCTTCCA AATCTTCCCG AGCTACCCCA
    AAATCTGCTA GAAGAAGCTT TGCGGAGGAA CCTTTACCCA AGGAGTTGGC AGAATATCTT
    GTGCCATACT GGGAGAACTT CTGTAGTTCA TATGTGACTA ATGTCAAAAC AGTGATGCAG
    AATCTCCGTG GTGAACGTGA GCTCATCATT CATCACCTTT ACAACATCCG GGAGAACTTC
    AGGCAGTATC TACAGAAGCC GGATCTGAAG CAGGAGTTTG TTAGCTCATG GCAGCGAGAT
    TATAACAGTG TTCCTGATAA CATCAGAGAG GACGAGGAGA CCAAAGGAGA GCTTCATCAA
    AGGCTGGATG ACTTGCGTGA GCGGCTATGG GACATCAGTG ATAAGCGTAA AGACGAGGCA
    GAGCAGGAGA AAGCAGGCGT CATTCATGAT GGCTGGTTAG AGGATCACAC CGCTGTACTA
    ATCAACAATT ATTCTGCATT GATGCAGATA GAAGTGGGCC GATTTCAGGA CTGTGTGTGT
    CTGCTGAAAG ATTATTATTC AACGATGGGT AAAACAGCCT TTGAATCCAA ATGTGACTTT
    GCTCGTGTCC CATTGCTTGA CATCACCGCT GATCAGCATG TCGACGAGTC GAAAATCTCT
    GAATCTGCTC CGTCAGAGAA ATCTGTAAAG AATGCTGAGG AAAAAGACTC TGAAATTGTG
    GACAGGATGA AAACTAAAGT GTTTCCACTG GTTTCCTGCA GATCTCCAAC TTCTGAGATC
    CTCAAACAGC CTTTGCATCA GGCTGATGAG AACCTGCTGC AGGAGTTCTT CCAAGCAGCA
    ATCGCCATCT TCAGGAGCAT GGTGTCGGCA GAGACGCGGC AGTGGGATGA AGAAGAAGTT
    GATGAAGACC AGAAGCAGAT GGAGGCACAG AAAGCTCAAA CTTTGACCTC TGCTACAGAC
    GTAAGGAAAG CAGGAAAACA AAAAGGTGCT CCACCTCCTC CTCCTCCAGA GCCCAGTCCT
    CAACCTGCTG CAAGGAACCC AGAGGAGCTC AGGAAGAGAG CGGAGAAGAA ACGATTCAAA
    CAGGAGCTCT CAGCTGCACT TAAACCTGAA GAAAATGCAG TGAAGCAGCG TTTGGAGCTA
    GTGAAGGCTC ATGGGCTGAG AACAGTCATC AGTCTGCAGC ACAAAGCAGA ACAGGTCTAT
    AGGAACATGG AGGAGTGGAT GAAAGCCCGC TATCTGGCTG AAATGAAAAG TATCGACCAG
    CTGACAGATT TGGTGAGGCA GCACATTGAG ACTGGAGTTC AGATCCGTCA TGAGCTGGTT
    CTGCAGAGCA CTGATTTCTG TGTGGATGGA GACACCCGTG TGCTGTTGAG TCCAGAGCTT
    ACTCCGCGCC GATCCCTGCT GGAGCAGCCT AAAAACAGCA CACTCACAGT ACAACAGCTG
    CACGTCCTCT GCACACAGTT GCGCAAGATC GCCCCTAAAG GTCTGATGTG TAGAAGTGAG
    CTGACTGAAG CTCTACACGA ATTAACCTCT GCTCACATGG GTACTGATGT CCTTCCTGAG
    GCCTGGATGC ACATCACTCC TTCACAGGTT GAGGAGCTGG TGTGTATTCT AGCTCAGGAC
    TCTGAGATGT TGGACTGGCG TCAGTTTCTG CTCAGTGCTG CTCTGCCGTG GCCATTTCCC
    ACTCAGACTC AGCTGATAAA AACCCTCCAG CGCTACAGAG ACATCGACAC CGCGGAAACC
    GGACTCATCA CACAAGAACA ATATGAACAG GTTGAATTAT GGTTTCCCAG TGAACAAGAT
    CCTTCTGTCC CTGATGACCC AACTGAACCC GTGCCCTATG ACAGATTGGC CAACCTTAAA
    AAGTTTTTCT TCAGTCTGTT TGCGAGCACA CACTCCTCAC CACTGCTGCT GGACTATCTG
    ATGATGCTGC TTCATTTCTG CTGCCATCCT GAAGCAGCTC AGGGCTTCAC CAGAGCGCTC
    GGCCTCGTCA CCGGACACAC ACTGCACTAC AAACACACCA CCCCACTCAC ACAGTCTGTG
    CCGTGTCTGG AGGGCGCTGG AGTTGTGGAT GATGCTGGTG CTGATAATGA AGAGGAGCAG
    CACAGAGGGG TGGAGTCTGA GGAAGTGGGC GTGTCTGTTG ATGATGTGCT CAGAGTTTTG
    AGTCATGGAG AGGATCTCGT CTTACATCTA AACCGCTTCC AGCTTGTCCA CAGGAGCAGA
    GATGAACTCC GAGAGGAGCT TCTGAAGGTG TTTAAGGACT TGGGTTTCAG TGGTGAAGAG
    AAGATTCCTT TCTCCACACT ATCTCAGCAT CCCTTTCTGC AGGACCTGAT AGAAGGATCC
    GCCTATTATC TGCTCCATGA CATTCACAAA ATTTTCCAGA CAAAACAAAC TGAAGATTCG
    AGAAGTTTCT CAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299456.1
    CDS125..5242
    Translation

    Target ORF information:

    RefSeq Version XM_009301181.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sperm flagellar 2 (spef2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301181.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGACA TTATCTGTCA GTGGCTGAAT TCAGAGCTCC GGCTGTCAAA AGTCGTCGAG 
    CCGCATTCAT TTGCCAGGGA TTTTTCAAAC GGTTATCTGA TAGGAGAGAT CCTCTACAGA
    TATGAGCTAC AAGACGATTT CCATCTGTTC TCAAAACAAA ACACATCGAA TGCCAAGCTA
    AATAACTTCA CTCGCATTGA GCCCACCCTG CAGCTGCTGA CCGTGCCCTT TGATCTGACC
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA AGAGCTTGGA GAAAAATGTT
    TCTCAGACAG AACATACTAC AGAACAGCTT CAGAAAGATG AGCAGGAGAC ATCCTCTCCT
    CTAGAATCAG GACCACAGCC TCATGAGGCC TCATCTGGGC TTCATGAAAA AGAGGACAAA
    GAAGAATCAA AGGCAATCCC AAAGTGGTCA TCACGAGCAC AGCACGGTGC AGCGGTAGAG
    AAAGTTCTGA GGACAGGCCA AGCTGTTCCT GATCAGCTTC TGGTGGACAT TATTGTGGAT
    GCTATCAGGA ACATTCCTGC AGATAGTGGC TGGATTCTTG ATGGCTTCCC GGTGGACATC
    AGTCAGGCTC AGATGCTTGA GAAAGCTCTA AACATGACTG AACTTGATGG AACAAAAACA
    CAAAATAATT TGTCCACAAA CAAAAACGGC CCTAAAGATG CGGCTCCTCC ATCCCCTGCT
    CTAGACCTAG TGGTGCTGTT AGACGTTTCA GATGAGCAGG TTCTGGAGCG AGCCACAACT
    CAGCCTCCTG AGGGAACACA GATAGTGAAT GGAGAAAGCA TAAAGCAGGA GAAAGGCAGC
    AGTGATGCTG CGGAGGAAAA CACAAGCATG CCAGATCTGA ACACACAAGA TCCTGAATCC
    TTGTCTGACG ACAAAAGCCT GGGGAGGACA CAAATACAGT TCAGAATCAG TGGCTTTCAT
    CAAACATGGC CAAAACTGGA GAAGTGGTTT GGTGATCAGC AGAATATTCT GGTGAAAGTC
    ACAGCAGGTG TGGACAAGGA CACTCTCTTC AGTAACGTGG AGACTATTCT GAGGGACACT
    ATTGAGTCTG TTGAAAAAGG AGCAGCTCTG GCTGACTCCA GGAGAGCTTC AGTGACCTCC
    ACACCTGAAC AGACCCGTCC AGAAAGTGCC TGCTCTTCCA AATCTTCCCG AGCTACCCCA
    AAATCTGCTA GAAGAAGCTT TGCGGAGGAA CCTTTACCCA AGGAGTTGGC AGAATATCTT
    GTGCCATACT GGGAGAACTT CTGTAGTTCA TATGTGACTA ATGTCAAAAC AGTGATGCAG
    AATCTCCGTG GTGAACGTGA GCTCATCATT CATCACCTTT ACAACATCCG GGAGAACTTC
    AGGCAGTATC TACAGAAGCC GGATCTGAAG CAGGAGTTTG TTAGCTCATG GCAGCGAGAT
    TATAACAGTG TTCCTGATAA CATCAGAGAG GACGAGGAGA CCAAAGGAGA GCTTCATCAA
    AGGCTGGATG ACTTGCGTGA GCGGCTATGG GACATCAGTG ATAAGCGTAA AGACGAGGCA
    GAGCAGGAGA AAGCAGGCGT CATTCATGAT GGCTGGTTAG AGGATCACAC CGCTGTACTA
    ATCAACAATT ATTCTGCATT GATGCAGATA GAAGTGGGCC GATTTCAGGA CTGTGTGTGT
    CTGCTGAAAG ATTATTATTC AACGATGGGT AAAACAGCCT TTGAATCCAA ATGTGACTTT
    GCTCGTGTCC CATTGCTTGA CATCACCGCT GATCAGCATG TCGACGAGTC GAAAATCTCT
    GAATCTGCTC CGTCAGAGAA ATCTGTAAAG AATGCTGAGG AAAAAGACTC TGAAATTGTG
    GACAGGATGA AAACTAAAGT GTTTCCACTG GTTTCCTGCA GATCTCCAAC TTCTGAGATC
    CTCAAACAGC CTTTGCATCA GGCTGATGAG AACCTGCTGC AGGAGTTCTT CCAAGCAGCA
    ATCGCCATCT TCAGGAGCAT GGTGTCGGCA GAGACGCGGC AGTGGGATGA AGAAGAAGTT
    GATGAAGACC AGAAGCAGAT GGAGGCACAG AAAGCTCAAA CTTTGACCTC TGCTACAGAC
    GTAAGGAAAG CAGGAAAACA AAAAGGTGCT CCACCTCCTC CTCCTCCAGA GCCCAGTCCT
    CAACCTGCTG CAAGGAACCC AGAGGAGCTC AGGAAGAGAG CGGAGAAGAA ACGATTCAAA
    CAGGAGCTCT CAGCTGCACT TAAACCTGAA GAAAATGCAG TGAAGCAGCG TTTGGAGCTA
    GTGAAGGCTC ATGGGCTGAG AACAGTCATC AGTCTGCAGC ACAAAGCAGA ACAGGTCTAT
    AGGAACATGG AGGAGTGGAT GAAAGCCCGC TATCTGGCTG AAATGAAAAG TATCGACCAG
    CTGACAGATT TGGTGAGGCA GCACATTGAG ACTGGAGTTC AGATCCGTCA TGAGCTGGTT
    CTGCAGAGCA CTGATTTCTG TGTGGATGGA GACACCCGTG TGCTGTTGAG TCCAGAGCTT
    ACTCCGCGCC GATCCCTGCT GGAGCAGCCT AAAAACAGCA CACTCACAGT ACAACAGCTG
    CACGTCCTCT GCACACAGTT GCGCAAGATC GCCCCTAAAG GTCTGATGTG TAGAAGTGAG
    CTGACTGAAG CTCTACACGA ATTAACCTCT GCTCACATGG GTACTGATGT CCTTCCTGAG
    GCCTGGATGC ACATCACTCC TTCACAGGTT GAGGAGCTGG TGTGTATTCT AGCTCAGGAC
    TCTGAGATGT TGGACTGGCG TCAGTTTCTG CTCAGTGCTG CTCTGCCGTG GCCATTTCCC
    ACTCAGACTC AGCTGATAAA AACCCTCCAG CGCTACAGAG ACATCGACAC CGCGGAAACC
    GGACTCATCA CACAAGAACA ATATGAACAG GTTGAATTAT GGTTTCCCAG TGAACAAGAT
    CCTTCTGTCC CTGATGACCC AACTGAACCC GTGCCCTATG ACAGATTGGC CAACCTTAAA
    AAGTTTTTCT TCAGTCTGTT TGCGAGCACA CACTCCTCAC CACTGCTGCT GGACTATCTG
    ATGATGCTGC TTCATTTCTG CTGCCATCCT GAAGCAGCTC AGGGCTTCAC CAGAGCGCTC
    GGCCTCGTCA CCGGACACAC ACTGCACTAC AAACACACCA CCCCACTCAC ACAGTCTGTG
    CCGTGTCTGG AGGGCGCTGG AGTTGTGGAT GATGCTGGTG CTGATAATGA AGAGGAGCAG
    CACAGAGGGG TGGAGTCTGA GGAAGTGGGC GTGTCTGTTG ATGATGTGCT CAGAGTTTTG
    AGTCATGGAG AGGATCTCGT CTTACATCTA AACCGCTTCC AGCTTGTCCA CAGGAGCAGA
    GATGAACTCC GAGAGGAGCT TCTGAAGGTG TTTAAGGACT TGGGTTTCAG TGGTGAAGAG
    AAGATTCCTT TCTCCACACT ATCTCAGCAT CCCTTTCTGC AGGACCTGAT AGAAGGATCC
    GCCTATTATC TGCTCCATGA CATTCACAAA ATTTTCCAGA CAAAACAAAC TGAAGATTCG
    AGAAGTTTCT CAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41924
    Clone ID Related Accession (Same CDS sequence) XM_009301181.3 , XM_009301181.2
    Accession Version XM_009301181.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5118bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product sperm flagellar protein 2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301181.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGACA TTATCTGTCA GTGGCTGAAT TCAGAGCTCC GGCTGTCAAA AGTCGTCGAG 
    CCGCATTCAT TTGCCAGGGA TTTTTCAAAC GGTTATCTGA TAGGAGAGAT CCTCTACAGA
    TATGAGCTAC AAGACGATTT CCATCTGTTC TCAAAACAAA ACACATCGAA TGCCAAGCTA
    AATAACTTCA CTCGCATTGA GCCCACCCTG CAGCTGCTGA CCGTGCCCTT TGATCTGACC
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA AGAGCTTGGA GAAAAATGTT
    TCTCAGACAG AACATACTAC AGAACAGCTT CAGAAAGATG AGCAGGAGAC ATCCTCTCCT
    CTAGAATCAG GACCACAGCC TCATGAGGCC TCATCTGGGC TTCATGAAAA AGAGGACAAA
    GAAGAATCAA AGGCAATCCC AAAGTGGTCA TCACGAGCAC AGCACGGTGC AGCGGTAGAG
    AAAGTTCTGA GGACAGGCCA AGCTGTTCCT GATCAGCTTC TGGTGGACAT TATTGTGGAT
    GCTATCAGGA ACATTCCTGC AGATAGTGGC TGGATTCTTG ATGGCTTCCC GGTGGACATC
    AGTCAGGCTC AGATGCTTGA GAAAGCTCTA AACATGACTG AACTTGATGG AACAAAAACA
    CAAAATAATT TGTCCACAAA CAAAAACGGC CCTAAAGATG CGGCTCCTCC ATCCCCTGCT
    CTAGACCTAG TGGTGCTGTT AGACGTTTCA GATGAGCAGG TTCTGGAGCG AGCCACAACT
    CAGCCTCCTG AGGGAACACA GATAGTGAAT GGAGAAAGCA TAAAGCAGGA GAAAGGCAGC
    AGTGATGCTG CGGAGGAAAA CACAAGCATG CCAGATCTGA ACACACAAGA TCCTGAATCC
    TTGTCTGACG ACAAAAGCCT GGGGAGGACA CAAATACAGT TCAGAATCAG TGGCTTTCAT
    CAAACATGGC CAAAACTGGA GAAGTGGTTT GGTGATCAGC AGAATATTCT GGTGAAAGTC
    ACAGCAGGTG TGGACAAGGA CACTCTCTTC AGTAACGTGG AGACTATTCT GAGGGACACT
    ATTGAGTCTG TTGAAAAAGG AGCAGCTCTG GCTGACTCCA GGAGAGCTTC AGTGACCTCC
    ACACCTGAAC AGACCCGTCC AGAAAGTGCC TGCTCTTCCA AATCTTCCCG AGCTACCCCA
    AAATCTGCTA GAAGAAGCTT TGCGGAGGAA CCTTTACCCA AGGAGTTGGC AGAATATCTT
    GTGCCATACT GGGAGAACTT CTGTAGTTCA TATGTGACTA ATGTCAAAAC AGTGATGCAG
    AATCTCCGTG GTGAACGTGA GCTCATCATT CATCACCTTT ACAACATCCG GGAGAACTTC
    AGGCAGTATC TACAGAAGCC GGATCTGAAG CAGGAGTTTG TTAGCTCATG GCAGCGAGAT
    TATAACAGTG TTCCTGATAA CATCAGAGAG GACGAGGAGA CCAAAGGAGA GCTTCATCAA
    AGGCTGGATG ACTTGCGTGA GCGGCTATGG GACATCAGTG ATAAGCGTAA AGACGAGGCA
    GAGCAGGAGA AAGCAGGCGT CATTCATGAT GGCTGGTTAG AGGATCACAC CGCTGTACTA
    ATCAACAATT ATTCTGCATT GATGCAGATA GAAGTGGGCC GATTTCAGGA CTGTGTGTGT
    CTGCTGAAAG ATTATTATTC AACGATGGGT AAAACAGCCT TTGAATCCAA ATGTGACTTT
    GCTCGTGTCC CATTGCTTGA CATCACCGCT GATCAGCATG TCGACGAGTC GAAAATCTCT
    GAATCTGCTC CGTCAGAGAA ATCTGTAAAG AATGCTGAGG AAAAAGACTC TGAAATTGTG
    GACAGGATGA AAACTAAAGT GTTTCCACTG GTTTCCTGCA GATCTCCAAC TTCTGAGATC
    CTCAAACAGC CTTTGCATCA GGCTGATGAG AACCTGCTGC AGGAGTTCTT CCAAGCAGCA
    ATCGCCATCT TCAGGAGCAT GGTGTCGGCA GAGACGCGGC AGTGGGATGA AGAAGAAGTT
    GATGAAGACC AGAAGCAGAT GGAGGCACAG AAAGCTCAAA CTTTGACCTC TGCTACAGAC
    GTAAGGAAAG CAGGAAAACA AAAAGGTGCT CCACCTCCTC CTCCTCCAGA GCCCAGTCCT
    CAACCTGCTG CAAGGAACCC AGAGGAGCTC AGGAAGAGAG CGGAGAAGAA ACGATTCAAA
    CAGGAGCTCT CAGCTGCACT TAAACCTGAA GAAAATGCAG TGAAGCAGCG TTTGGAGCTA
    GTGAAGGCTC ATGGGCTGAG AACAGTCATC AGTCTGCAGC ACAAAGCAGA ACAGGTCTAT
    AGGAACATGG AGGAGTGGAT GAAAGCCCGC TATCTGGCTG AAATGAAAAG TATCGACCAG
    CTGACAGATT TGGTGAGGCA GCACATTGAG ACTGGAGTTC AGATCCGTCA TGAGCTGGTT
    CTGCAGAGCA CTGATTTCTG TGTGGATGGA GACACCCGTG TGCTGTTGAG TCCAGAGCTT
    ACTCCGCGCC GATCCCTGCT GGAGCAGCCT AAAAACAGCA CACTCACAGT ACAACAGCTG
    CACGTCCTCT GCACACAGTT GCGCAAGATC GCCCCTAAAG GTCTGATGTG TAGAAGTGAG
    CTGACTGAAG CTCTACACGA ATTAACCTCT GCTCACATGG GTACTGATGT CCTTCCTGAG
    GCCTGGATGC ACATCACTCC TTCACAGGTT GAGGAGCTGG TGTGTATTCT AGCTCAGGAC
    TCTGAGATGT TGGACTGGCG TCAGTTTCTG CTCAGTGCTG CTCTGCCGTG GCCATTTCCC
    ACTCAGACTC AGCTGATAAA AACCCTCCAG CGCTACAGAG ACATCGACAC CGCGGAAACC
    GGACTCATCA CACAAGAACA ATATGAACAG GTTGAATTAT GGTTTCCCAG TGAACAAGAT
    CCTTCTGTCC CTGATGACCC AACTGAACCC GTGCCCTATG ACAGATTGGC CAACCTTAAA
    AAGTTTTTCT TCAGTCTGTT TGCGAGCACA CACTCCTCAC CACTGCTGCT GGACTATCTG
    ATGATGCTGC TTCATTTCTG CTGCCATCCT GAAGCAGCTC AGGGCTTCAC CAGAGCGCTC
    GGCCTCGTCA CCGGACACAC ACTGCACTAC AAACACACCA CCCCACTCAC ACAGTCTGTG
    CCGTGTCTGG AGGGCGCTGG AGTTGTGGAT GATGCTGGTG CTGATAATGA AGAGGAGCAG
    CACAGAGGGG TGGAGTCTGA GGAAGTGGGC GTGTCTGTTG ATGATGTGCT CAGAGTTTTG
    AGTCATGGAG AGGATCTCGT CTTACATCTA AACCGCTTCC AGCTTGTCCA CAGGAGCAGA
    GATGAACTCC GAGAGGAGCT TCTGAAGGTG TTTAAGGACT TGGGTTTCAG TGGTGAAGAG
    AAGATTCCTT TCTCCACACT ATCTCAGCAT CCCTTTCTGC AGGACCTGAT AGAAGGATCC
    GCCTATTATC TGCTCCATGA CATTCACAAA ATTTTCCAGA CAAAACAAAC TGAAGATTCG
    AGAAGTTTCT CAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299456.1
    CDS130..5247
    Translation

    Target ORF information:

    RefSeq Version XM_009301181.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sperm flagellar 2 (spef2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301181.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGACA TTATCTGTCA GTGGCTGAAT TCAGAGCTCC GGCTGTCAAA AGTCGTCGAG 
    CCGCATTCAT TTGCCAGGGA TTTTTCAAAC GGTTATCTGA TAGGAGAGAT CCTCTACAGA
    TATGAGCTAC AAGACGATTT CCATCTGTTC TCAAAACAAA ACACATCGAA TGCCAAGCTA
    AATAACTTCA CTCGCATTGA GCCCACCCTG CAGCTGCTGA CCGTGCCCTT TGATCTGACC
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA AGAGCTTGGA GAAAAATGTT
    TCTCAGACAG AACATACTAC AGAACAGCTT CAGAAAGATG AGCAGGAGAC ATCCTCTCCT
    CTAGAATCAG GACCACAGCC TCATGAGGCC TCATCTGGGC TTCATGAAAA AGAGGACAAA
    GAAGAATCAA AGGCAATCCC AAAGTGGTCA TCACGAGCAC AGCACGGTGC AGCGGTAGAG
    AAAGTTCTGA GGACAGGCCA AGCTGTTCCT GATCAGCTTC TGGTGGACAT TATTGTGGAT
    GCTATCAGGA ACATTCCTGC AGATAGTGGC TGGATTCTTG ATGGCTTCCC GGTGGACATC
    AGTCAGGCTC AGATGCTTGA GAAAGCTCTA AACATGACTG AACTTGATGG AACAAAAACA
    CAAAATAATT TGTCCACAAA CAAAAACGGC CCTAAAGATG CGGCTCCTCC ATCCCCTGCT
    CTAGACCTAG TGGTGCTGTT AGACGTTTCA GATGAGCAGG TTCTGGAGCG AGCCACAACT
    CAGCCTCCTG AGGGAACACA GATAGTGAAT GGAGAAAGCA TAAAGCAGGA GAAAGGCAGC
    AGTGATGCTG CGGAGGAAAA CACAAGCATG CCAGATCTGA ACACACAAGA TCCTGAATCC
    TTGTCTGACG ACAAAAGCCT GGGGAGGACA CAAATACAGT TCAGAATCAG TGGCTTTCAT
    CAAACATGGC CAAAACTGGA GAAGTGGTTT GGTGATCAGC AGAATATTCT GGTGAAAGTC
    ACAGCAGGTG TGGACAAGGA CACTCTCTTC AGTAACGTGG AGACTATTCT GAGGGACACT
    ATTGAGTCTG TTGAAAAAGG AGCAGCTCTG GCTGACTCCA GGAGAGCTTC AGTGACCTCC
    ACACCTGAAC AGACCCGTCC AGAAAGTGCC TGCTCTTCCA AATCTTCCCG AGCTACCCCA
    AAATCTGCTA GAAGAAGCTT TGCGGAGGAA CCTTTACCCA AGGAGTTGGC AGAATATCTT
    GTGCCATACT GGGAGAACTT CTGTAGTTCA TATGTGACTA ATGTCAAAAC AGTGATGCAG
    AATCTCCGTG GTGAACGTGA GCTCATCATT CATCACCTTT ACAACATCCG GGAGAACTTC
    AGGCAGTATC TACAGAAGCC GGATCTGAAG CAGGAGTTTG TTAGCTCATG GCAGCGAGAT
    TATAACAGTG TTCCTGATAA CATCAGAGAG GACGAGGAGA CCAAAGGAGA GCTTCATCAA
    AGGCTGGATG ACTTGCGTGA GCGGCTATGG GACATCAGTG ATAAGCGTAA AGACGAGGCA
    GAGCAGGAGA AAGCAGGCGT CATTCATGAT GGCTGGTTAG AGGATCACAC CGCTGTACTA
    ATCAACAATT ATTCTGCATT GATGCAGATA GAAGTGGGCC GATTTCAGGA CTGTGTGTGT
    CTGCTGAAAG ATTATTATTC AACGATGGGT AAAACAGCCT TTGAATCCAA ATGTGACTTT
    GCTCGTGTCC CATTGCTTGA CATCACCGCT GATCAGCATG TCGACGAGTC GAAAATCTCT
    GAATCTGCTC CGTCAGAGAA ATCTGTAAAG AATGCTGAGG AAAAAGACTC TGAAATTGTG
    GACAGGATGA AAACTAAAGT GTTTCCACTG GTTTCCTGCA GATCTCCAAC TTCTGAGATC
    CTCAAACAGC CTTTGCATCA GGCTGATGAG AACCTGCTGC AGGAGTTCTT CCAAGCAGCA
    ATCGCCATCT TCAGGAGCAT GGTGTCGGCA GAGACGCGGC AGTGGGATGA AGAAGAAGTT
    GATGAAGACC AGAAGCAGAT GGAGGCACAG AAAGCTCAAA CTTTGACCTC TGCTACAGAC
    GTAAGGAAAG CAGGAAAACA AAAAGGTGCT CCACCTCCTC CTCCTCCAGA GCCCAGTCCT
    CAACCTGCTG CAAGGAACCC AGAGGAGCTC AGGAAGAGAG CGGAGAAGAA ACGATTCAAA
    CAGGAGCTCT CAGCTGCACT TAAACCTGAA GAAAATGCAG TGAAGCAGCG TTTGGAGCTA
    GTGAAGGCTC ATGGGCTGAG AACAGTCATC AGTCTGCAGC ACAAAGCAGA ACAGGTCTAT
    AGGAACATGG AGGAGTGGAT GAAAGCCCGC TATCTGGCTG AAATGAAAAG TATCGACCAG
    CTGACAGATT TGGTGAGGCA GCACATTGAG ACTGGAGTTC AGATCCGTCA TGAGCTGGTT
    CTGCAGAGCA CTGATTTCTG TGTGGATGGA GACACCCGTG TGCTGTTGAG TCCAGAGCTT
    ACTCCGCGCC GATCCCTGCT GGAGCAGCCT AAAAACAGCA CACTCACAGT ACAACAGCTG
    CACGTCCTCT GCACACAGTT GCGCAAGATC GCCCCTAAAG GTCTGATGTG TAGAAGTGAG
    CTGACTGAAG CTCTACACGA ATTAACCTCT GCTCACATGG GTACTGATGT CCTTCCTGAG
    GCCTGGATGC ACATCACTCC TTCACAGGTT GAGGAGCTGG TGTGTATTCT AGCTCAGGAC
    TCTGAGATGT TGGACTGGCG TCAGTTTCTG CTCAGTGCTG CTCTGCCGTG GCCATTTCCC
    ACTCAGACTC AGCTGATAAA AACCCTCCAG CGCTACAGAG ACATCGACAC CGCGGAAACC
    GGACTCATCA CACAAGAACA ATATGAACAG GTTGAATTAT GGTTTCCCAG TGAACAAGAT
    CCTTCTGTCC CTGATGACCC AACTGAACCC GTGCCCTATG ACAGATTGGC CAACCTTAAA
    AAGTTTTTCT TCAGTCTGTT TGCGAGCACA CACTCCTCAC CACTGCTGCT GGACTATCTG
    ATGATGCTGC TTCATTTCTG CTGCCATCCT GAAGCAGCTC AGGGCTTCAC CAGAGCGCTC
    GGCCTCGTCA CCGGACACAC ACTGCACTAC AAACACACCA CCCCACTCAC ACAGTCTGTG
    CCGTGTCTGG AGGGCGCTGG AGTTGTGGAT GATGCTGGTG CTGATAATGA AGAGGAGCAG
    CACAGAGGGG TGGAGTCTGA GGAAGTGGGC GTGTCTGTTG ATGATGTGCT CAGAGTTTTG
    AGTCATGGAG AGGATCTCGT CTTACATCTA AACCGCTTCC AGCTTGTCCA CAGGAGCAGA
    GATGAACTCC GAGAGGAGCT TCTGAAGGTG TTTAAGGACT TGGGTTTCAG TGGTGAAGAG
    AAGATTCCTT TCTCCACACT ATCTCAGCAT CCCTTTCTGC AGGACCTGAT AGAAGGATCC
    GCCTATTATC TGCTCCATGA CATTCACAAA ATTTTCCAGA CAAAACAAAC TGAAGATTCG
    AGAAGTTTCT CAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41925
    Clone ID Related Accession (Same CDS sequence) XM_009301182.3 , XM_009301182.2
    Accession Version XM_009301182.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5115bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product sperm flagellar protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301182.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGACA TTATCTGTCA GTGGCTGAAT TCAGAGCTCC GGCTGTCAAA AGTCGTCGAG 
    CCGCATTCAT TTGCCAGGGA TTTTTCAAAC GGTTATCTGA TAGGAGAGAT CCTCTACAGA
    TATGAGCTAC AAGACGATTT CCATCTGTTC TCAAAACAAA ACACATCGAA TGCCAAGCTA
    AATAACTTCA CTCGCATTGA GCCCACCCTG CAGCTGCTGA CCGTGCCCTT TGATCTGACC
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA GCTTGGAGAA AAATGTTTCT
    CAGACAGAAC ATACTACAGA ACAGCTTCAG AAAGATGAGC AGGAGACATC CTCTCCTCTA
    GAATCAGGAC CACAGCCTCA TGAGGCCTCA TCTGGGCTTC ATGAAAAAGA GGACAAAGAA
    GAATCAAAGG CAATCCCAAA GTGGTCATCA CGAGCACAGC ACGGTGCAGC GGTAGAGAAA
    GTTCTGAGGA CAGGCCAAGC TGTTCCTGAT CAGCTTCTGG TGGACATTAT TGTGGATGCT
    ATCAGGAACA TTCCTGCAGA TAGTGGCTGG ATTCTTGATG GCTTCCCGGT GGACATCAGT
    CAGGCTCAGA TGCTTGAGAA AGCTCTAAAC ATGACTGAAC TTGATGGAAC AAAAACACAA
    AATAATTTGT CCACAAACAA AAACGGCCCT AAAGATGCGG CTCCTCCATC CCCTGCTCTA
    GACCTAGTGG TGCTGTTAGA CGTTTCAGAT GAGCAGGTTC TGGAGCGAGC CACAACTCAG
    CCTCCTGAGG GAACACAGAT AGTGAATGGA GAAAGCATAA AGCAGGAGAA AGGCAGCAGT
    GATGCTGCGG AGGAAAACAC AAGCATGCCA GATCTGAACA CACAAGATCC TGAATCCTTG
    TCTGACGACA AAAGCCTGGG GAGGACACAA ATACAGTTCA GAATCAGTGG CTTTCATCAA
    ACATGGCCAA AACTGGAGAA GTGGTTTGGT GATCAGCAGA ATATTCTGGT GAAAGTCACA
    GCAGGTGTGG ACAAGGACAC TCTCTTCAGT AACGTGGAGA CTATTCTGAG GGACACTATT
    GAGTCTGTTG AAAAAGGAGC AGCTCTGGCT GACTCCAGGA GAGCTTCAGT GACCTCCACA
    CCTGAACAGA CCCGTCCAGA AAGTGCCTGC TCTTCCAAAT CTTCCCGAGC TACCCCAAAA
    TCTGCTAGAA GAAGCTTTGC GGAGGAACCT TTACCCAAGG AGTTGGCAGA ATATCTTGTG
    CCATACTGGG AGAACTTCTG TAGTTCATAT GTGACTAATG TCAAAACAGT GATGCAGAAT
    CTCCGTGGTG AACGTGAGCT CATCATTCAT CACCTTTACA ACATCCGGGA GAACTTCAGG
    CAGTATCTAC AGAAGCCGGA TCTGAAGCAG GAGTTTGTTA GCTCATGGCA GCGAGATTAT
    AACAGTGTTC CTGATAACAT CAGAGAGGAC GAGGAGACCA AAGGAGAGCT TCATCAAAGG
    CTGGATGACT TGCGTGAGCG GCTATGGGAC ATCAGTGATA AGCGTAAAGA CGAGGCAGAG
    CAGGAGAAAG CAGGCGTCAT TCATGATGGC TGGTTAGAGG ATCACACCGC TGTACTAATC
    AACAATTATT CTGCATTGAT GCAGATAGAA GTGGGCCGAT TTCAGGACTG TGTGTGTCTG
    CTGAAAGATT ATTATTCAAC GATGGGTAAA ACAGCCTTTG AATCCAAATG TGACTTTGCT
    CGTGTCCCAT TGCTTGACAT CACCGCTGAT CAGCATGTCG ACGAGTCGAA AATCTCTGAA
    TCTGCTCCGT CAGAGAAATC TGTAAAGAAT GCTGAGGAAA AAGACTCTGA AATTGTGGAC
    AGGATGAAAA CTAAAGTGTT TCCACTGGTT TCCTGCAGAT CTCCAACTTC TGAGATCCTC
    AAACAGCCTT TGCATCAGGC TGATGAGAAC CTGCTGCAGG AGTTCTTCCA AGCAGCAATC
    GCCATCTTCA GGAGCATGGT GTCGGCAGAG ACGCGGCAGT GGGATGAAGA AGAAGTTGAT
    GAAGACCAGA AGCAGATGGA GGCACAGAAA GCTCAAACTT TGACCTCTGC TACAGACGTA
    AGGAAAGCAG GAAAACAAAA AGGTGCTCCA CCTCCTCCTC CTCCAGAGCC CAGTCCTCAA
    CCTGCTGCAA GGAACCCAGA GGAGCTCAGG AAGAGAGCGG AGAAGAAACG ATTCAAACAG
    GAGCTCTCAG CTGCACTTAA ACCTGAAGAA AATGCAGTGA AGCAGCGTTT GGAGCTAGTG
    AAGGCTCATG GGCTGAGAAC AGTCATCAGT CTGCAGCACA AAGCAGAACA GGTCTATAGG
    AACATGGAGG AGTGGATGAA AGCCCGCTAT CTGGCTGAAA TGAAAAGTAT CGACCAGCTG
    ACAGATTTGG TGAGGCAGCA CATTGAGACT GGAGTTCAGA TCCGTCATGA GCTGGTTCTG
    CAGAGCACTG ATTTCTGTGT GGATGGAGAC ACCCGTGTGC TGTTGAGTCC AGAGCTTACT
    CCGCGCCGAT CCCTGCTGGA GCAGCCTAAA AACAGCACAC TCACAGTACA ACAGCTGCAC
    GTCCTCTGCA CACAGTTGCG CAAGATCGCC CCTAAAGGTC TGATGTGTAG AAGTGAGCTG
    ACTGAAGCTC TACACGAATT AACCTCTGCT CACATGGGTA CTGATGTCCT TCCTGAGGCC
    TGGATGCACA TCACTCCTTC ACAGGTTGAG GAGCTGGTGT GTATTCTAGC TCAGGACTCT
    GAGATGTTGG ACTGGCGTCA GTTTCTGCTC AGTGCTGCTC TGCCGTGGCC ATTTCCCACT
    CAGACTCAGC TGATAAAAAC CCTCCAGCGC TACAGAGACA TCGACACCGC GGAAACCGGA
    CTCATCACAC AAGAACAATA TGAACAGGTT GAATTATGGT TTCCCAGTGA ACAAGATCCT
    TCTGTCCCTG ATGACCCAAC TGAACCCGTG CCCTATGACA GATTGGCCAA CCTTAAAAAG
    TTTTTCTTCA GTCTGTTTGC GAGCACACAC TCCTCACCAC TGCTGCTGGA CTATCTGATG
    ATGCTGCTTC ATTTCTGCTG CCATCCTGAA GCAGCTCAGG GCTTCACCAG AGCGCTCGGC
    CTCGTCACCG GACACACACT GCACTACAAA CACACCACCC CACTCACACA GTCTGTGCCG
    TGTCTGGAGG GCGCTGGAGT TGTGGATGAT GCTGGTGCTG ATAATGAAGA GGAGCAGCAC
    AGAGGGGTGG AGTCTGAGGA AGTGGGCGTG TCTGTTGATG ATGTGCTCAG AGTTTTGAGT
    CATGGAGAGG ATCTCGTCTT ACATCTAAAC CGCTTCCAGC TTGTCCACAG GAGCAGAGAT
    GAACTCCGAG AGGAGCTTCT GAAGGTGTTT AAGGACTTGG GTTTCAGTGG TGAAGAGAAG
    ATTCCTTTCT CCACACTATC TCAGCATCCC TTTCTGCAGG ACCTGATAGA AGGATCCGCC
    TATTATCTGC TCCATGACAT TCACAAAATT TTCCAGACAA AACAAACTGA AGATTCGAGA
    AGTTTCTCAA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299457.1
    CDS125..5239
    Translation

    Target ORF information:

    RefSeq Version XM_009301182.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sperm flagellar 2 (spef2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301182.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGACA TTATCTGTCA GTGGCTGAAT TCAGAGCTCC GGCTGTCAAA AGTCGTCGAG 
    CCGCATTCAT TTGCCAGGGA TTTTTCAAAC GGTTATCTGA TAGGAGAGAT CCTCTACAGA
    TATGAGCTAC AAGACGATTT CCATCTGTTC TCAAAACAAA ACACATCGAA TGCCAAGCTA
    AATAACTTCA CTCGCATTGA GCCCACCCTG CAGCTGCTGA CCGTGCCCTT TGATCTGACC
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA GCTTGGAGAA AAATGTTTCT
    CAGACAGAAC ATACTACAGA ACAGCTTCAG AAAGATGAGC AGGAGACATC CTCTCCTCTA
    GAATCAGGAC CACAGCCTCA TGAGGCCTCA TCTGGGCTTC ATGAAAAAGA GGACAAAGAA
    GAATCAAAGG CAATCCCAAA GTGGTCATCA CGAGCACAGC ACGGTGCAGC GGTAGAGAAA
    GTTCTGAGGA CAGGCCAAGC TGTTCCTGAT CAGCTTCTGG TGGACATTAT TGTGGATGCT
    ATCAGGAACA TTCCTGCAGA TAGTGGCTGG ATTCTTGATG GCTTCCCGGT GGACATCAGT
    CAGGCTCAGA TGCTTGAGAA AGCTCTAAAC ATGACTGAAC TTGATGGAAC AAAAACACAA
    AATAATTTGT CCACAAACAA AAACGGCCCT AAAGATGCGG CTCCTCCATC CCCTGCTCTA
    GACCTAGTGG TGCTGTTAGA CGTTTCAGAT GAGCAGGTTC TGGAGCGAGC CACAACTCAG
    CCTCCTGAGG GAACACAGAT AGTGAATGGA GAAAGCATAA AGCAGGAGAA AGGCAGCAGT
    GATGCTGCGG AGGAAAACAC AAGCATGCCA GATCTGAACA CACAAGATCC TGAATCCTTG
    TCTGACGACA AAAGCCTGGG GAGGACACAA ATACAGTTCA GAATCAGTGG CTTTCATCAA
    ACATGGCCAA AACTGGAGAA GTGGTTTGGT GATCAGCAGA ATATTCTGGT GAAAGTCACA
    GCAGGTGTGG ACAAGGACAC TCTCTTCAGT AACGTGGAGA CTATTCTGAG GGACACTATT
    GAGTCTGTTG AAAAAGGAGC AGCTCTGGCT GACTCCAGGA GAGCTTCAGT GACCTCCACA
    CCTGAACAGA CCCGTCCAGA AAGTGCCTGC TCTTCCAAAT CTTCCCGAGC TACCCCAAAA
    TCTGCTAGAA GAAGCTTTGC GGAGGAACCT TTACCCAAGG AGTTGGCAGA ATATCTTGTG
    CCATACTGGG AGAACTTCTG TAGTTCATAT GTGACTAATG TCAAAACAGT GATGCAGAAT
    CTCCGTGGTG AACGTGAGCT CATCATTCAT CACCTTTACA ACATCCGGGA GAACTTCAGG
    CAGTATCTAC AGAAGCCGGA TCTGAAGCAG GAGTTTGTTA GCTCATGGCA GCGAGATTAT
    AACAGTGTTC CTGATAACAT CAGAGAGGAC GAGGAGACCA AAGGAGAGCT TCATCAAAGG
    CTGGATGACT TGCGTGAGCG GCTATGGGAC ATCAGTGATA AGCGTAAAGA CGAGGCAGAG
    CAGGAGAAAG CAGGCGTCAT TCATGATGGC TGGTTAGAGG ATCACACCGC TGTACTAATC
    AACAATTATT CTGCATTGAT GCAGATAGAA GTGGGCCGAT TTCAGGACTG TGTGTGTCTG
    CTGAAAGATT ATTATTCAAC GATGGGTAAA ACAGCCTTTG AATCCAAATG TGACTTTGCT
    CGTGTCCCAT TGCTTGACAT CACCGCTGAT CAGCATGTCG ACGAGTCGAA AATCTCTGAA
    TCTGCTCCGT CAGAGAAATC TGTAAAGAAT GCTGAGGAAA AAGACTCTGA AATTGTGGAC
    AGGATGAAAA CTAAAGTGTT TCCACTGGTT TCCTGCAGAT CTCCAACTTC TGAGATCCTC
    AAACAGCCTT TGCATCAGGC TGATGAGAAC CTGCTGCAGG AGTTCTTCCA AGCAGCAATC
    GCCATCTTCA GGAGCATGGT GTCGGCAGAG ACGCGGCAGT GGGATGAAGA AGAAGTTGAT
    GAAGACCAGA AGCAGATGGA GGCACAGAAA GCTCAAACTT TGACCTCTGC TACAGACGTA
    AGGAAAGCAG GAAAACAAAA AGGTGCTCCA CCTCCTCCTC CTCCAGAGCC CAGTCCTCAA
    CCTGCTGCAA GGAACCCAGA GGAGCTCAGG AAGAGAGCGG AGAAGAAACG ATTCAAACAG
    GAGCTCTCAG CTGCACTTAA ACCTGAAGAA AATGCAGTGA AGCAGCGTTT GGAGCTAGTG
    AAGGCTCATG GGCTGAGAAC AGTCATCAGT CTGCAGCACA AAGCAGAACA GGTCTATAGG
    AACATGGAGG AGTGGATGAA AGCCCGCTAT CTGGCTGAAA TGAAAAGTAT CGACCAGCTG
    ACAGATTTGG TGAGGCAGCA CATTGAGACT GGAGTTCAGA TCCGTCATGA GCTGGTTCTG
    CAGAGCACTG ATTTCTGTGT GGATGGAGAC ACCCGTGTGC TGTTGAGTCC AGAGCTTACT
    CCGCGCCGAT CCCTGCTGGA GCAGCCTAAA AACAGCACAC TCACAGTACA ACAGCTGCAC
    GTCCTCTGCA CACAGTTGCG CAAGATCGCC CCTAAAGGTC TGATGTGTAG AAGTGAGCTG
    ACTGAAGCTC TACACGAATT AACCTCTGCT CACATGGGTA CTGATGTCCT TCCTGAGGCC
    TGGATGCACA TCACTCCTTC ACAGGTTGAG GAGCTGGTGT GTATTCTAGC TCAGGACTCT
    GAGATGTTGG ACTGGCGTCA GTTTCTGCTC AGTGCTGCTC TGCCGTGGCC ATTTCCCACT
    CAGACTCAGC TGATAAAAAC CCTCCAGCGC TACAGAGACA TCGACACCGC GGAAACCGGA
    CTCATCACAC AAGAACAATA TGAACAGGTT GAATTATGGT TTCCCAGTGA ACAAGATCCT
    TCTGTCCCTG ATGACCCAAC TGAACCCGTG CCCTATGACA GATTGGCCAA CCTTAAAAAG
    TTTTTCTTCA GTCTGTTTGC GAGCACACAC TCCTCACCAC TGCTGCTGGA CTATCTGATG
    ATGCTGCTTC ATTTCTGCTG CCATCCTGAA GCAGCTCAGG GCTTCACCAG AGCGCTCGGC
    CTCGTCACCG GACACACACT GCACTACAAA CACACCACCC CACTCACACA GTCTGTGCCG
    TGTCTGGAGG GCGCTGGAGT TGTGGATGAT GCTGGTGCTG ATAATGAAGA GGAGCAGCAC
    AGAGGGGTGG AGTCTGAGGA AGTGGGCGTG TCTGTTGATG ATGTGCTCAG AGTTTTGAGT
    CATGGAGAGG ATCTCGTCTT ACATCTAAAC CGCTTCCAGC TTGTCCACAG GAGCAGAGAT
    GAACTCCGAG AGGAGCTTCT GAAGGTGTTT AAGGACTTGG GTTTCAGTGG TGAAGAGAAG
    ATTCCTTTCT CCACACTATC TCAGCATCCC TTTCTGCAGG ACCTGATAGA AGGATCCGCC
    TATTATCTGC TCCATGACAT TCACAAAATT TTCCAGACAA AACAAACTGA AGATTCGAGA
    AGTTTCTCAA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41925
    Clone ID Related Accession (Same CDS sequence) XM_009301182.3 , XM_009301182.2
    Accession Version XM_009301182.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5115bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product sperm flagellar protein 2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301182.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGACA TTATCTGTCA GTGGCTGAAT TCAGAGCTCC GGCTGTCAAA AGTCGTCGAG 
    CCGCATTCAT TTGCCAGGGA TTTTTCAAAC GGTTATCTGA TAGGAGAGAT CCTCTACAGA
    TATGAGCTAC AAGACGATTT CCATCTGTTC TCAAAACAAA ACACATCGAA TGCCAAGCTA
    AATAACTTCA CTCGCATTGA GCCCACCCTG CAGCTGCTGA CCGTGCCCTT TGATCTGACC
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA GCTTGGAGAA AAATGTTTCT
    CAGACAGAAC ATACTACAGA ACAGCTTCAG AAAGATGAGC AGGAGACATC CTCTCCTCTA
    GAATCAGGAC CACAGCCTCA TGAGGCCTCA TCTGGGCTTC ATGAAAAAGA GGACAAAGAA
    GAATCAAAGG CAATCCCAAA GTGGTCATCA CGAGCACAGC ACGGTGCAGC GGTAGAGAAA
    GTTCTGAGGA CAGGCCAAGC TGTTCCTGAT CAGCTTCTGG TGGACATTAT TGTGGATGCT
    ATCAGGAACA TTCCTGCAGA TAGTGGCTGG ATTCTTGATG GCTTCCCGGT GGACATCAGT
    CAGGCTCAGA TGCTTGAGAA AGCTCTAAAC ATGACTGAAC TTGATGGAAC AAAAACACAA
    AATAATTTGT CCACAAACAA AAACGGCCCT AAAGATGCGG CTCCTCCATC CCCTGCTCTA
    GACCTAGTGG TGCTGTTAGA CGTTTCAGAT GAGCAGGTTC TGGAGCGAGC CACAACTCAG
    CCTCCTGAGG GAACACAGAT AGTGAATGGA GAAAGCATAA AGCAGGAGAA AGGCAGCAGT
    GATGCTGCGG AGGAAAACAC AAGCATGCCA GATCTGAACA CACAAGATCC TGAATCCTTG
    TCTGACGACA AAAGCCTGGG GAGGACACAA ATACAGTTCA GAATCAGTGG CTTTCATCAA
    ACATGGCCAA AACTGGAGAA GTGGTTTGGT GATCAGCAGA ATATTCTGGT GAAAGTCACA
    GCAGGTGTGG ACAAGGACAC TCTCTTCAGT AACGTGGAGA CTATTCTGAG GGACACTATT
    GAGTCTGTTG AAAAAGGAGC AGCTCTGGCT GACTCCAGGA GAGCTTCAGT GACCTCCACA
    CCTGAACAGA CCCGTCCAGA AAGTGCCTGC TCTTCCAAAT CTTCCCGAGC TACCCCAAAA
    TCTGCTAGAA GAAGCTTTGC GGAGGAACCT TTACCCAAGG AGTTGGCAGA ATATCTTGTG
    CCATACTGGG AGAACTTCTG TAGTTCATAT GTGACTAATG TCAAAACAGT GATGCAGAAT
    CTCCGTGGTG AACGTGAGCT CATCATTCAT CACCTTTACA ACATCCGGGA GAACTTCAGG
    CAGTATCTAC AGAAGCCGGA TCTGAAGCAG GAGTTTGTTA GCTCATGGCA GCGAGATTAT
    AACAGTGTTC CTGATAACAT CAGAGAGGAC GAGGAGACCA AAGGAGAGCT TCATCAAAGG
    CTGGATGACT TGCGTGAGCG GCTATGGGAC ATCAGTGATA AGCGTAAAGA CGAGGCAGAG
    CAGGAGAAAG CAGGCGTCAT TCATGATGGC TGGTTAGAGG ATCACACCGC TGTACTAATC
    AACAATTATT CTGCATTGAT GCAGATAGAA GTGGGCCGAT TTCAGGACTG TGTGTGTCTG
    CTGAAAGATT ATTATTCAAC GATGGGTAAA ACAGCCTTTG AATCCAAATG TGACTTTGCT
    CGTGTCCCAT TGCTTGACAT CACCGCTGAT CAGCATGTCG ACGAGTCGAA AATCTCTGAA
    TCTGCTCCGT CAGAGAAATC TGTAAAGAAT GCTGAGGAAA AAGACTCTGA AATTGTGGAC
    AGGATGAAAA CTAAAGTGTT TCCACTGGTT TCCTGCAGAT CTCCAACTTC TGAGATCCTC
    AAACAGCCTT TGCATCAGGC TGATGAGAAC CTGCTGCAGG AGTTCTTCCA AGCAGCAATC
    GCCATCTTCA GGAGCATGGT GTCGGCAGAG ACGCGGCAGT GGGATGAAGA AGAAGTTGAT
    GAAGACCAGA AGCAGATGGA GGCACAGAAA GCTCAAACTT TGACCTCTGC TACAGACGTA
    AGGAAAGCAG GAAAACAAAA AGGTGCTCCA CCTCCTCCTC CTCCAGAGCC CAGTCCTCAA
    CCTGCTGCAA GGAACCCAGA GGAGCTCAGG AAGAGAGCGG AGAAGAAACG ATTCAAACAG
    GAGCTCTCAG CTGCACTTAA ACCTGAAGAA AATGCAGTGA AGCAGCGTTT GGAGCTAGTG
    AAGGCTCATG GGCTGAGAAC AGTCATCAGT CTGCAGCACA AAGCAGAACA GGTCTATAGG
    AACATGGAGG AGTGGATGAA AGCCCGCTAT CTGGCTGAAA TGAAAAGTAT CGACCAGCTG
    ACAGATTTGG TGAGGCAGCA CATTGAGACT GGAGTTCAGA TCCGTCATGA GCTGGTTCTG
    CAGAGCACTG ATTTCTGTGT GGATGGAGAC ACCCGTGTGC TGTTGAGTCC AGAGCTTACT
    CCGCGCCGAT CCCTGCTGGA GCAGCCTAAA AACAGCACAC TCACAGTACA ACAGCTGCAC
    GTCCTCTGCA CACAGTTGCG CAAGATCGCC CCTAAAGGTC TGATGTGTAG AAGTGAGCTG
    ACTGAAGCTC TACACGAATT AACCTCTGCT CACATGGGTA CTGATGTCCT TCCTGAGGCC
    TGGATGCACA TCACTCCTTC ACAGGTTGAG GAGCTGGTGT GTATTCTAGC TCAGGACTCT
    GAGATGTTGG ACTGGCGTCA GTTTCTGCTC AGTGCTGCTC TGCCGTGGCC ATTTCCCACT
    CAGACTCAGC TGATAAAAAC CCTCCAGCGC TACAGAGACA TCGACACCGC GGAAACCGGA
    CTCATCACAC AAGAACAATA TGAACAGGTT GAATTATGGT TTCCCAGTGA ACAAGATCCT
    TCTGTCCCTG ATGACCCAAC TGAACCCGTG CCCTATGACA GATTGGCCAA CCTTAAAAAG
    TTTTTCTTCA GTCTGTTTGC GAGCACACAC TCCTCACCAC TGCTGCTGGA CTATCTGATG
    ATGCTGCTTC ATTTCTGCTG CCATCCTGAA GCAGCTCAGG GCTTCACCAG AGCGCTCGGC
    CTCGTCACCG GACACACACT GCACTACAAA CACACCACCC CACTCACACA GTCTGTGCCG
    TGTCTGGAGG GCGCTGGAGT TGTGGATGAT GCTGGTGCTG ATAATGAAGA GGAGCAGCAC
    AGAGGGGTGG AGTCTGAGGA AGTGGGCGTG TCTGTTGATG ATGTGCTCAG AGTTTTGAGT
    CATGGAGAGG ATCTCGTCTT ACATCTAAAC CGCTTCCAGC TTGTCCACAG GAGCAGAGAT
    GAACTCCGAG AGGAGCTTCT GAAGGTGTTT AAGGACTTGG GTTTCAGTGG TGAAGAGAAG
    ATTCCTTTCT CCACACTATC TCAGCATCCC TTTCTGCAGG ACCTGATAGA AGGATCCGCC
    TATTATCTGC TCCATGACAT TCACAAAATT TTCCAGACAA AACAAACTGA AGATTCGAGA
    AGTTTCTCAA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299457.1
    CDS130..5244
    Translation

    Target ORF information:

    RefSeq Version XM_009301182.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sperm flagellar 2 (spef2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301182.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGACA TTATCTGTCA GTGGCTGAAT TCAGAGCTCC GGCTGTCAAA AGTCGTCGAG 
    CCGCATTCAT TTGCCAGGGA TTTTTCAAAC GGTTATCTGA TAGGAGAGAT CCTCTACAGA
    TATGAGCTAC AAGACGATTT CCATCTGTTC TCAAAACAAA ACACATCGAA TGCCAAGCTA
    AATAACTTCA CTCGCATTGA GCCCACCCTG CAGCTGCTGA CCGTGCCCTT TGATCTGACC
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA GCTTGGAGAA AAATGTTTCT
    CAGACAGAAC ATACTACAGA ACAGCTTCAG AAAGATGAGC AGGAGACATC CTCTCCTCTA
    GAATCAGGAC CACAGCCTCA TGAGGCCTCA TCTGGGCTTC ATGAAAAAGA GGACAAAGAA
    GAATCAAAGG CAATCCCAAA GTGGTCATCA CGAGCACAGC ACGGTGCAGC GGTAGAGAAA
    GTTCTGAGGA CAGGCCAAGC TGTTCCTGAT CAGCTTCTGG TGGACATTAT TGTGGATGCT
    ATCAGGAACA TTCCTGCAGA TAGTGGCTGG ATTCTTGATG GCTTCCCGGT GGACATCAGT
    CAGGCTCAGA TGCTTGAGAA AGCTCTAAAC ATGACTGAAC TTGATGGAAC AAAAACACAA
    AATAATTTGT CCACAAACAA AAACGGCCCT AAAGATGCGG CTCCTCCATC CCCTGCTCTA
    GACCTAGTGG TGCTGTTAGA CGTTTCAGAT GAGCAGGTTC TGGAGCGAGC CACAACTCAG
    CCTCCTGAGG GAACACAGAT AGTGAATGGA GAAAGCATAA AGCAGGAGAA AGGCAGCAGT
    GATGCTGCGG AGGAAAACAC AAGCATGCCA GATCTGAACA CACAAGATCC TGAATCCTTG
    TCTGACGACA AAAGCCTGGG GAGGACACAA ATACAGTTCA GAATCAGTGG CTTTCATCAA
    ACATGGCCAA AACTGGAGAA GTGGTTTGGT GATCAGCAGA ATATTCTGGT GAAAGTCACA
    GCAGGTGTGG ACAAGGACAC TCTCTTCAGT AACGTGGAGA CTATTCTGAG GGACACTATT
    GAGTCTGTTG AAAAAGGAGC AGCTCTGGCT GACTCCAGGA GAGCTTCAGT GACCTCCACA
    CCTGAACAGA CCCGTCCAGA AAGTGCCTGC TCTTCCAAAT CTTCCCGAGC TACCCCAAAA
    TCTGCTAGAA GAAGCTTTGC GGAGGAACCT TTACCCAAGG AGTTGGCAGA ATATCTTGTG
    CCATACTGGG AGAACTTCTG TAGTTCATAT GTGACTAATG TCAAAACAGT GATGCAGAAT
    CTCCGTGGTG AACGTGAGCT CATCATTCAT CACCTTTACA ACATCCGGGA GAACTTCAGG
    CAGTATCTAC AGAAGCCGGA TCTGAAGCAG GAGTTTGTTA GCTCATGGCA GCGAGATTAT
    AACAGTGTTC CTGATAACAT CAGAGAGGAC GAGGAGACCA AAGGAGAGCT TCATCAAAGG
    CTGGATGACT TGCGTGAGCG GCTATGGGAC ATCAGTGATA AGCGTAAAGA CGAGGCAGAG
    CAGGAGAAAG CAGGCGTCAT TCATGATGGC TGGTTAGAGG ATCACACCGC TGTACTAATC
    AACAATTATT CTGCATTGAT GCAGATAGAA GTGGGCCGAT TTCAGGACTG TGTGTGTCTG
    CTGAAAGATT ATTATTCAAC GATGGGTAAA ACAGCCTTTG AATCCAAATG TGACTTTGCT
    CGTGTCCCAT TGCTTGACAT CACCGCTGAT CAGCATGTCG ACGAGTCGAA AATCTCTGAA
    TCTGCTCCGT CAGAGAAATC TGTAAAGAAT GCTGAGGAAA AAGACTCTGA AATTGTGGAC
    AGGATGAAAA CTAAAGTGTT TCCACTGGTT TCCTGCAGAT CTCCAACTTC TGAGATCCTC
    AAACAGCCTT TGCATCAGGC TGATGAGAAC CTGCTGCAGG AGTTCTTCCA AGCAGCAATC
    GCCATCTTCA GGAGCATGGT GTCGGCAGAG ACGCGGCAGT GGGATGAAGA AGAAGTTGAT
    GAAGACCAGA AGCAGATGGA GGCACAGAAA GCTCAAACTT TGACCTCTGC TACAGACGTA
    AGGAAAGCAG GAAAACAAAA AGGTGCTCCA CCTCCTCCTC CTCCAGAGCC CAGTCCTCAA
    CCTGCTGCAA GGAACCCAGA GGAGCTCAGG AAGAGAGCGG AGAAGAAACG ATTCAAACAG
    GAGCTCTCAG CTGCACTTAA ACCTGAAGAA AATGCAGTGA AGCAGCGTTT GGAGCTAGTG
    AAGGCTCATG GGCTGAGAAC AGTCATCAGT CTGCAGCACA AAGCAGAACA GGTCTATAGG
    AACATGGAGG AGTGGATGAA AGCCCGCTAT CTGGCTGAAA TGAAAAGTAT CGACCAGCTG
    ACAGATTTGG TGAGGCAGCA CATTGAGACT GGAGTTCAGA TCCGTCATGA GCTGGTTCTG
    CAGAGCACTG ATTTCTGTGT GGATGGAGAC ACCCGTGTGC TGTTGAGTCC AGAGCTTACT
    CCGCGCCGAT CCCTGCTGGA GCAGCCTAAA AACAGCACAC TCACAGTACA ACAGCTGCAC
    GTCCTCTGCA CACAGTTGCG CAAGATCGCC CCTAAAGGTC TGATGTGTAG AAGTGAGCTG
    ACTGAAGCTC TACACGAATT AACCTCTGCT CACATGGGTA CTGATGTCCT TCCTGAGGCC
    TGGATGCACA TCACTCCTTC ACAGGTTGAG GAGCTGGTGT GTATTCTAGC TCAGGACTCT
    GAGATGTTGG ACTGGCGTCA GTTTCTGCTC AGTGCTGCTC TGCCGTGGCC ATTTCCCACT
    CAGACTCAGC TGATAAAAAC CCTCCAGCGC TACAGAGACA TCGACACCGC GGAAACCGGA
    CTCATCACAC AAGAACAATA TGAACAGGTT GAATTATGGT TTCCCAGTGA ACAAGATCCT
    TCTGTCCCTG ATGACCCAAC TGAACCCGTG CCCTATGACA GATTGGCCAA CCTTAAAAAG
    TTTTTCTTCA GTCTGTTTGC GAGCACACAC TCCTCACCAC TGCTGCTGGA CTATCTGATG
    ATGCTGCTTC ATTTCTGCTG CCATCCTGAA GCAGCTCAGG GCTTCACCAG AGCGCTCGGC
    CTCGTCACCG GACACACACT GCACTACAAA CACACCACCC CACTCACACA GTCTGTGCCG
    TGTCTGGAGG GCGCTGGAGT TGTGGATGAT GCTGGTGCTG ATAATGAAGA GGAGCAGCAC
    AGAGGGGTGG AGTCTGAGGA AGTGGGCGTG TCTGTTGATG ATGTGCTCAG AGTTTTGAGT
    CATGGAGAGG ATCTCGTCTT ACATCTAAAC CGCTTCCAGC TTGTCCACAG GAGCAGAGAT
    GAACTCCGAG AGGAGCTTCT GAAGGTGTTT AAGGACTTGG GTTTCAGTGG TGAAGAGAAG
    ATTCCTTTCT CCACACTATC TCAGCATCCC TTTCTGCAGG ACCTGATAGA AGGATCCGCC
    TATTATCTGC TCCATGACAT TCACAAAATT TTCCAGACAA AACAAACTGA AGATTCGAGA
    AGTTTCTCAA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41926
    Clone ID Related Accession (Same CDS sequence) XM_009301183.2 , XM_009301183.3
    Accession Version XM_009301183.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product sperm flagellar protein 2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301183.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGACA TTATCTGTCA GTGGCTGAAT TCAGAGCTCC GGCTGTCAAA AGTCGTCGAG 
    CCGCATTCAT TTGCCAGGGA TTTTTCAAAC GGTTATCTGA TAGGAGAGAT CCTCTACAGA
    TATGAGCTAC AAGACGATTT CCATCTGTTC TCAAAACAAA ACACATCGAA TGCCAAGCTA
    AATAACTTCA CTCGCATTGA GCCCACCCTG CAGCTGCTGA CCGTGCCCTT TGATCTGACC
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA AGAGCTTGGA GAAAAATGTT
    TCTCAGACAG AACATACTAC AGAACAGCTT CAGAAAGATG AGCAGGAGAC ATCCTCTCCT
    CTAGAATCAG GACCACAGCC TCATGAGGCC TCATCTGGGC TTCATGAAAA AGAGGACAAA
    GAAGAATCAA AGGCAATCCC AAAGTGGTCA TCACGAGCAC AGCACGGTGC AGCGGTAGAG
    AAAGTTCTGA GGACAGGCCA AGCTGTTCCT GATCAGCTTC TGGTGGACAT TATTGTGGAT
    GCTATCAGGA ACATTCCTGC AGATAGTGGC TGGATTCTTG ATGGCTTCCC GGTGGACATC
    AGTCAGGCTC AGATGCTTGA GAAAGCTCTA AACATGACTG AACTTGATGG AACAAAAACA
    CAAAATAATT TGTCCACAAA CAAAAACGGC CCTAAAGATG CGGCTCCTCC ATCCCCTGCT
    CTAGACCTAG TGGTGCTGTT AGACGTTTCA GATGAGCAGG TTCTGGAGCG AGCCACAACT
    CAGCCTCCTG AGGGAACACA GATAGTGAAT GGAGAAAGCA TAAAGCAGGA GAAAGGCAGC
    AGTGATGCTG CGGAGGAAAA CACAAGCATG CCAGATCTGA ACACACAAGA TCCTGAATCC
    TTGTCTGACG ACAAAAGCCT GGGGAGGACA CAAATACAGT TCAGAATCAG TGGCTTTCAT
    CAAACATGGC CAAAACTGGA GAAGTGGTTT GGTGATCAGC AGAATATTCT GGTGAAAGTC
    ACAGCAGGTG TGGACAAGGA CACTCTCTTC AGTAACGTGG AGACTATTCT GAGGGACACT
    ATTGAGTCTG TTGAAAAAGC TCTGGCTGAC TCCAGGAGAG CTTCAGTGAC CTCCACACCT
    GAACAGACCC GTCCAGAAAG TGCCTGCTCT TCCAAATCTT CCCGAGCTAC CCCAAAATCT
    GCTAGAAGAA GCTTTGCGGA GGAACCTTTA CCCAAGGAGT TGGCAGAATA TCTTGTGCCA
    TACTGGGAGA ACTTCTGTAG TTCATATGTG ACTAATGTCA AAACAGTGAT GCAGAATCTC
    CGTGGTGAAC GTGAGCTCAT CATTCATCAC CTTTACAACA TCCGGGAGAA CTTCAGGCAG
    TATCTACAGA AGCCGGATCT GAAGCAGGAG TTTGTTAGCT CATGGCAGCG AGATTATAAC
    AGTGTTCCTG ATAACATCAG AGAGGACGAG GAGACCAAAG GAGAGCTTCA TCAAAGGCTG
    GATGACTTGC GTGAGCGGCT ATGGGACATC AGTGATAAGC GTAAAGACGA GGCAGAGCAG
    GAGAAAGCAG GCGTCATTCA TGATGGCTGG TTAGAGGATC ACACCGCTGT ACTAATCAAC
    AATTATTCTG CATTGATGCA GATAGAAGTG GGCCGATTTC AGGACTGTGT GTGTCTGCTG
    AAAGATTATT ATTCAACGAT GGGTAAAACA GCCTTTGAAT CCAAATGTGA CTTTGCTCGT
    GTCCCATTGC TTGACATCAC CGCTGATCAG CATGTCGACG AGTCGAAAAT CTCTGAATCT
    GCTCCGTCAG AGAAATCTGT AAAGAATGCT GAGGAAAAAG ACTCTGAAAT TGTGGACAGG
    ATGAAAACTA AAGTGTTTCC ACTGGTTTCC TGCAGATCTC CAACTTCTGA GATCCTCAAA
    CAGCCTTTGC ATCAGGCTGA TGAGAACCTG CTGCAGGAGT TCTTCCAAGC AGCAATCGCC
    ATCTTCAGGA GCATGGTGTC GGCAGAGACG CGGCAGTGGG ATGAAGAAGA AGTTGATGAA
    GACCAGAAGC AGATGGAGGC ACAGAAAGCT CAAACTTTGA CCTCTGCTAC AGACGTAAGG
    AAAGCAGGAA AACAAAAAGG TGCTCCACCT CCTCCTCCTC CAGAGCCCAG TCCTCAACCT
    GCTGCAAGGA ACCCAGAGGA GCTCAGGAAG AGAGCGGAGA AGAAACGATT CAAACAGGAG
    CTCTCAGCTG CACTTAAACC TGAAGAAAAT GCAGTGAAGC AGCGTTTGGA GCTAGTGAAG
    GCTCATGGGC TGAGAACAGT CATCAGTCTG CAGCACAAAG CAGAACAGGT CTATAGGAAC
    ATGGAGGAGT GGATGAAAGC CCGCTATCTG GCTGAAATGA AAAGTATCGA CCAGCTGACA
    GATTTGGTGA GGCAGCACAT TGAGACTGGA GTTCAGATCC GTCATGAGCT GGTTCTGCAG
    AGCACTGATT TCTGTGTGGA TGGAGACACC CGTGTGCTGT TGAGTCCAGA GCTTACTCCG
    CGCCGATCCC TGCTGGAGCA GCCTAAAAAC AGCACACTCA CAGTACAACA GCTGCACGTC
    CTCTGCACAC AGTTGCGCAA GATCGCCCCT AAAGGTCTGA TGTGTAGAAG TGAGCTGACT
    GAAGCTCTAC ACGAATTAAC CTCTGCTCAC ATGGGTACTG ATGTCCTTCC TGAGGCCTGG
    ATGCACATCA CTCCTTCACA GGTTGAGGAG CTGGTGTGTA TTCTAGCTCA GGACTCTGAG
    ATGTTGGACT GGCGTCAGTT TCTGCTCAGT GCTGCTCTGC CGTGGCCATT TCCCACTCAG
    ACTCAGCTGA TAAAAACCCT CCAGCGCTAC AGAGACATCG ACACCGCGGA AACCGGACTC
    ATCACACAAG AACAATATGA ACAGGTTGAA TTATGGTTTC CCAGTGAACA AGATCCTTCT
    GTCCCTGATG ACCCAACTGA ACCCGTGCCC TATGACAGAT TGGCCAACCT TAAAAAGTTT
    TTCTTCAGTC TGTTTGCGAG CACACACTCC TCACCACTGC TGCTGGACTA TCTGATGATG
    CTGCTTCATT TCTGCTGCCA TCCTGAAGCA GCTCAGGGCT TCACCAGAGC GCTCGGCCTC
    GTCACCGGAC ACACACTGCA CTACAAACAC ACCACCCCAC TCACACAGTC TGTGCCGTGT
    CTGGAGGGCG CTGGAGTTGT GGATGATGCT GGTGCTGATA ATGAAGAGGA GCAGCACAGA
    GGGGTGGAGT CTGAGGAAGT GGGCGTGTCT GTTGATGATG TGCTCAGAGT TTTGAGTCAT
    GGAGAGGATC TCGTCTTACA TCTAAACCGC TTCCAGCTTG TCCACAGGAG CAGAGATGAA
    CTCCGAGAGG AGCTTCTGAA GGTGTTTAAG GACTTGGGTT TCAGTGGTGA AGAGAAGATT
    CCTTTCTCCA CACTATCTCA GCATCCCTTT CTGCAGGACC TGATAGAAGG ATCCGCCTAT
    TATCTGCTCC ATGACATTCA CAAAATTTTC CAGACAAAAC AAACTGAAGA TTCGAGAAGT
    TTCTCAACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299458.1
    CDS130..5241
    Translation

    Target ORF information:

    RefSeq Version XM_009301183.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sperm flagellar 2 (spef2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301183.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGACA TTATCTGTCA GTGGCTGAAT TCAGAGCTCC GGCTGTCAAA AGTCGTCGAG 
    CCGCATTCAT TTGCCAGGGA TTTTTCAAAC GGTTATCTGA TAGGAGAGAT CCTCTACAGA
    TATGAGCTAC AAGACGATTT CCATCTGTTC TCAAAACAAA ACACATCGAA TGCCAAGCTA
    AATAACTTCA CTCGCATTGA GCCCACCCTG CAGCTGCTGA CCGTGCCCTT TGATCTGACC
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA AGAGCTTGGA GAAAAATGTT
    TCTCAGACAG AACATACTAC AGAACAGCTT CAGAAAGATG AGCAGGAGAC ATCCTCTCCT
    CTAGAATCAG GACCACAGCC TCATGAGGCC TCATCTGGGC TTCATGAAAA AGAGGACAAA
    GAAGAATCAA AGGCAATCCC AAAGTGGTCA TCACGAGCAC AGCACGGTGC AGCGGTAGAG
    AAAGTTCTGA GGACAGGCCA AGCTGTTCCT GATCAGCTTC TGGTGGACAT TATTGTGGAT
    GCTATCAGGA ACATTCCTGC AGATAGTGGC TGGATTCTTG ATGGCTTCCC GGTGGACATC
    AGTCAGGCTC AGATGCTTGA GAAAGCTCTA AACATGACTG AACTTGATGG AACAAAAACA
    CAAAATAATT TGTCCACAAA CAAAAACGGC CCTAAAGATG CGGCTCCTCC ATCCCCTGCT
    CTAGACCTAG TGGTGCTGTT AGACGTTTCA GATGAGCAGG TTCTGGAGCG AGCCACAACT
    CAGCCTCCTG AGGGAACACA GATAGTGAAT GGAGAAAGCA TAAAGCAGGA GAAAGGCAGC
    AGTGATGCTG CGGAGGAAAA CACAAGCATG CCAGATCTGA ACACACAAGA TCCTGAATCC
    TTGTCTGACG ACAAAAGCCT GGGGAGGACA CAAATACAGT TCAGAATCAG TGGCTTTCAT
    CAAACATGGC CAAAACTGGA GAAGTGGTTT GGTGATCAGC AGAATATTCT GGTGAAAGTC
    ACAGCAGGTG TGGACAAGGA CACTCTCTTC AGTAACGTGG AGACTATTCT GAGGGACACT
    ATTGAGTCTG TTGAAAAAGC TCTGGCTGAC TCCAGGAGAG CTTCAGTGAC CTCCACACCT
    GAACAGACCC GTCCAGAAAG TGCCTGCTCT TCCAAATCTT CCCGAGCTAC CCCAAAATCT
    GCTAGAAGAA GCTTTGCGGA GGAACCTTTA CCCAAGGAGT TGGCAGAATA TCTTGTGCCA
    TACTGGGAGA ACTTCTGTAG TTCATATGTG ACTAATGTCA AAACAGTGAT GCAGAATCTC
    CGTGGTGAAC GTGAGCTCAT CATTCATCAC CTTTACAACA TCCGGGAGAA CTTCAGGCAG
    TATCTACAGA AGCCGGATCT GAAGCAGGAG TTTGTTAGCT CATGGCAGCG AGATTATAAC
    AGTGTTCCTG ATAACATCAG AGAGGACGAG GAGACCAAAG GAGAGCTTCA TCAAAGGCTG
    GATGACTTGC GTGAGCGGCT ATGGGACATC AGTGATAAGC GTAAAGACGA GGCAGAGCAG
    GAGAAAGCAG GCGTCATTCA TGATGGCTGG TTAGAGGATC ACACCGCTGT ACTAATCAAC
    AATTATTCTG CATTGATGCA GATAGAAGTG GGCCGATTTC AGGACTGTGT GTGTCTGCTG
    AAAGATTATT ATTCAACGAT GGGTAAAACA GCCTTTGAAT CCAAATGTGA CTTTGCTCGT
    GTCCCATTGC TTGACATCAC CGCTGATCAG CATGTCGACG AGTCGAAAAT CTCTGAATCT
    GCTCCGTCAG AGAAATCTGT AAAGAATGCT GAGGAAAAAG ACTCTGAAAT TGTGGACAGG
    ATGAAAACTA AAGTGTTTCC ACTGGTTTCC TGCAGATCTC CAACTTCTGA GATCCTCAAA
    CAGCCTTTGC ATCAGGCTGA TGAGAACCTG CTGCAGGAGT TCTTCCAAGC AGCAATCGCC
    ATCTTCAGGA GCATGGTGTC GGCAGAGACG CGGCAGTGGG ATGAAGAAGA AGTTGATGAA
    GACCAGAAGC AGATGGAGGC ACAGAAAGCT CAAACTTTGA CCTCTGCTAC AGACGTAAGG
    AAAGCAGGAA AACAAAAAGG TGCTCCACCT CCTCCTCCTC CAGAGCCCAG TCCTCAACCT
    GCTGCAAGGA ACCCAGAGGA GCTCAGGAAG AGAGCGGAGA AGAAACGATT CAAACAGGAG
    CTCTCAGCTG CACTTAAACC TGAAGAAAAT GCAGTGAAGC AGCGTTTGGA GCTAGTGAAG
    GCTCATGGGC TGAGAACAGT CATCAGTCTG CAGCACAAAG CAGAACAGGT CTATAGGAAC
    ATGGAGGAGT GGATGAAAGC CCGCTATCTG GCTGAAATGA AAAGTATCGA CCAGCTGACA
    GATTTGGTGA GGCAGCACAT TGAGACTGGA GTTCAGATCC GTCATGAGCT GGTTCTGCAG
    AGCACTGATT TCTGTGTGGA TGGAGACACC CGTGTGCTGT TGAGTCCAGA GCTTACTCCG
    CGCCGATCCC TGCTGGAGCA GCCTAAAAAC AGCACACTCA CAGTACAACA GCTGCACGTC
    CTCTGCACAC AGTTGCGCAA GATCGCCCCT AAAGGTCTGA TGTGTAGAAG TGAGCTGACT
    GAAGCTCTAC ACGAATTAAC CTCTGCTCAC ATGGGTACTG ATGTCCTTCC TGAGGCCTGG
    ATGCACATCA CTCCTTCACA GGTTGAGGAG CTGGTGTGTA TTCTAGCTCA GGACTCTGAG
    ATGTTGGACT GGCGTCAGTT TCTGCTCAGT GCTGCTCTGC CGTGGCCATT TCCCACTCAG
    ACTCAGCTGA TAAAAACCCT CCAGCGCTAC AGAGACATCG ACACCGCGGA AACCGGACTC
    ATCACACAAG AACAATATGA ACAGGTTGAA TTATGGTTTC CCAGTGAACA AGATCCTTCT
    GTCCCTGATG ACCCAACTGA ACCCGTGCCC TATGACAGAT TGGCCAACCT TAAAAAGTTT
    TTCTTCAGTC TGTTTGCGAG CACACACTCC TCACCACTGC TGCTGGACTA TCTGATGATG
    CTGCTTCATT TCTGCTGCCA TCCTGAAGCA GCTCAGGGCT TCACCAGAGC GCTCGGCCTC
    GTCACCGGAC ACACACTGCA CTACAAACAC ACCACCCCAC TCACACAGTC TGTGCCGTGT
    CTGGAGGGCG CTGGAGTTGT GGATGATGCT GGTGCTGATA ATGAAGAGGA GCAGCACAGA
    GGGGTGGAGT CTGAGGAAGT GGGCGTGTCT GTTGATGATG TGCTCAGAGT TTTGAGTCAT
    GGAGAGGATC TCGTCTTACA TCTAAACCGC TTCCAGCTTG TCCACAGGAG CAGAGATGAA
    CTCCGAGAGG AGCTTCTGAA GGTGTTTAAG GACTTGGGTT TCAGTGGTGA AGAGAAGATT
    CCTTTCTCCA CACTATCTCA GCATCCCTTT CTGCAGGACC TGATAGAAGG ATCCGCCTAT
    TATCTGCTCC ATGACATTCA CAAAATTTTC CAGACAAAAC AAACTGAAGA TTCGAGAAGT
    TTCTCAACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41926
    Clone ID Related Accession (Same CDS sequence) XM_009301183.2 , XM_009301183.3
    Accession Version XM_009301183.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product sperm flagellar protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301183.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGACA TTATCTGTCA GTGGCTGAAT TCAGAGCTCC GGCTGTCAAA AGTCGTCGAG 
    CCGCATTCAT TTGCCAGGGA TTTTTCAAAC GGTTATCTGA TAGGAGAGAT CCTCTACAGA
    TATGAGCTAC AAGACGATTT CCATCTGTTC TCAAAACAAA ACACATCGAA TGCCAAGCTA
    AATAACTTCA CTCGCATTGA GCCCACCCTG CAGCTGCTGA CCGTGCCCTT TGATCTGACC
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA AGAGCTTGGA GAAAAATGTT
    TCTCAGACAG AACATACTAC AGAACAGCTT CAGAAAGATG AGCAGGAGAC ATCCTCTCCT
    CTAGAATCAG GACCACAGCC TCATGAGGCC TCATCTGGGC TTCATGAAAA AGAGGACAAA
    GAAGAATCAA AGGCAATCCC AAAGTGGTCA TCACGAGCAC AGCACGGTGC AGCGGTAGAG
    AAAGTTCTGA GGACAGGCCA AGCTGTTCCT GATCAGCTTC TGGTGGACAT TATTGTGGAT
    GCTATCAGGA ACATTCCTGC AGATAGTGGC TGGATTCTTG ATGGCTTCCC GGTGGACATC
    AGTCAGGCTC AGATGCTTGA GAAAGCTCTA AACATGACTG AACTTGATGG AACAAAAACA
    CAAAATAATT TGTCCACAAA CAAAAACGGC CCTAAAGATG CGGCTCCTCC ATCCCCTGCT
    CTAGACCTAG TGGTGCTGTT AGACGTTTCA GATGAGCAGG TTCTGGAGCG AGCCACAACT
    CAGCCTCCTG AGGGAACACA GATAGTGAAT GGAGAAAGCA TAAAGCAGGA GAAAGGCAGC
    AGTGATGCTG CGGAGGAAAA CACAAGCATG CCAGATCTGA ACACACAAGA TCCTGAATCC
    TTGTCTGACG ACAAAAGCCT GGGGAGGACA CAAATACAGT TCAGAATCAG TGGCTTTCAT
    CAAACATGGC CAAAACTGGA GAAGTGGTTT GGTGATCAGC AGAATATTCT GGTGAAAGTC
    ACAGCAGGTG TGGACAAGGA CACTCTCTTC AGTAACGTGG AGACTATTCT GAGGGACACT
    ATTGAGTCTG TTGAAAAAGC TCTGGCTGAC TCCAGGAGAG CTTCAGTGAC CTCCACACCT
    GAACAGACCC GTCCAGAAAG TGCCTGCTCT TCCAAATCTT CCCGAGCTAC CCCAAAATCT
    GCTAGAAGAA GCTTTGCGGA GGAACCTTTA CCCAAGGAGT TGGCAGAATA TCTTGTGCCA
    TACTGGGAGA ACTTCTGTAG TTCATATGTG ACTAATGTCA AAACAGTGAT GCAGAATCTC
    CGTGGTGAAC GTGAGCTCAT CATTCATCAC CTTTACAACA TCCGGGAGAA CTTCAGGCAG
    TATCTACAGA AGCCGGATCT GAAGCAGGAG TTTGTTAGCT CATGGCAGCG AGATTATAAC
    AGTGTTCCTG ATAACATCAG AGAGGACGAG GAGACCAAAG GAGAGCTTCA TCAAAGGCTG
    GATGACTTGC GTGAGCGGCT ATGGGACATC AGTGATAAGC GTAAAGACGA GGCAGAGCAG
    GAGAAAGCAG GCGTCATTCA TGATGGCTGG TTAGAGGATC ACACCGCTGT ACTAATCAAC
    AATTATTCTG CATTGATGCA GATAGAAGTG GGCCGATTTC AGGACTGTGT GTGTCTGCTG
    AAAGATTATT ATTCAACGAT GGGTAAAACA GCCTTTGAAT CCAAATGTGA CTTTGCTCGT
    GTCCCATTGC TTGACATCAC CGCTGATCAG CATGTCGACG AGTCGAAAAT CTCTGAATCT
    GCTCCGTCAG AGAAATCTGT AAAGAATGCT GAGGAAAAAG ACTCTGAAAT TGTGGACAGG
    ATGAAAACTA AAGTGTTTCC ACTGGTTTCC TGCAGATCTC CAACTTCTGA GATCCTCAAA
    CAGCCTTTGC ATCAGGCTGA TGAGAACCTG CTGCAGGAGT TCTTCCAAGC AGCAATCGCC
    ATCTTCAGGA GCATGGTGTC GGCAGAGACG CGGCAGTGGG ATGAAGAAGA AGTTGATGAA
    GACCAGAAGC AGATGGAGGC ACAGAAAGCT CAAACTTTGA CCTCTGCTAC AGACGTAAGG
    AAAGCAGGAA AACAAAAAGG TGCTCCACCT CCTCCTCCTC CAGAGCCCAG TCCTCAACCT
    GCTGCAAGGA ACCCAGAGGA GCTCAGGAAG AGAGCGGAGA AGAAACGATT CAAACAGGAG
    CTCTCAGCTG CACTTAAACC TGAAGAAAAT GCAGTGAAGC AGCGTTTGGA GCTAGTGAAG
    GCTCATGGGC TGAGAACAGT CATCAGTCTG CAGCACAAAG CAGAACAGGT CTATAGGAAC
    ATGGAGGAGT GGATGAAAGC CCGCTATCTG GCTGAAATGA AAAGTATCGA CCAGCTGACA
    GATTTGGTGA GGCAGCACAT TGAGACTGGA GTTCAGATCC GTCATGAGCT GGTTCTGCAG
    AGCACTGATT TCTGTGTGGA TGGAGACACC CGTGTGCTGT TGAGTCCAGA GCTTACTCCG
    CGCCGATCCC TGCTGGAGCA GCCTAAAAAC AGCACACTCA CAGTACAACA GCTGCACGTC
    CTCTGCACAC AGTTGCGCAA GATCGCCCCT AAAGGTCTGA TGTGTAGAAG TGAGCTGACT
    GAAGCTCTAC ACGAATTAAC CTCTGCTCAC ATGGGTACTG ATGTCCTTCC TGAGGCCTGG
    ATGCACATCA CTCCTTCACA GGTTGAGGAG CTGGTGTGTA TTCTAGCTCA GGACTCTGAG
    ATGTTGGACT GGCGTCAGTT TCTGCTCAGT GCTGCTCTGC CGTGGCCATT TCCCACTCAG
    ACTCAGCTGA TAAAAACCCT CCAGCGCTAC AGAGACATCG ACACCGCGGA AACCGGACTC
    ATCACACAAG AACAATATGA ACAGGTTGAA TTATGGTTTC CCAGTGAACA AGATCCTTCT
    GTCCCTGATG ACCCAACTGA ACCCGTGCCC TATGACAGAT TGGCCAACCT TAAAAAGTTT
    TTCTTCAGTC TGTTTGCGAG CACACACTCC TCACCACTGC TGCTGGACTA TCTGATGATG
    CTGCTTCATT TCTGCTGCCA TCCTGAAGCA GCTCAGGGCT TCACCAGAGC GCTCGGCCTC
    GTCACCGGAC ACACACTGCA CTACAAACAC ACCACCCCAC TCACACAGTC TGTGCCGTGT
    CTGGAGGGCG CTGGAGTTGT GGATGATGCT GGTGCTGATA ATGAAGAGGA GCAGCACAGA
    GGGGTGGAGT CTGAGGAAGT GGGCGTGTCT GTTGATGATG TGCTCAGAGT TTTGAGTCAT
    GGAGAGGATC TCGTCTTACA TCTAAACCGC TTCCAGCTTG TCCACAGGAG CAGAGATGAA
    CTCCGAGAGG AGCTTCTGAA GGTGTTTAAG GACTTGGGTT TCAGTGGTGA AGAGAAGATT
    CCTTTCTCCA CACTATCTCA GCATCCCTTT CTGCAGGACC TGATAGAAGG ATCCGCCTAT
    TATCTGCTCC ATGACATTCA CAAAATTTTC CAGACAAAAC AAACTGAAGA TTCGAGAAGT
    TTCTCAACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299458.1
    CDS125..5236
    Translation

    Target ORF information:

    RefSeq Version XM_009301183.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sperm flagellar 2 (spef2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301183.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGACA TTATCTGTCA GTGGCTGAAT TCAGAGCTCC GGCTGTCAAA AGTCGTCGAG 
    CCGCATTCAT TTGCCAGGGA TTTTTCAAAC GGTTATCTGA TAGGAGAGAT CCTCTACAGA
    TATGAGCTAC AAGACGATTT CCATCTGTTC TCAAAACAAA ACACATCGAA TGCCAAGCTA
    AATAACTTCA CTCGCATTGA GCCCACCCTG CAGCTGCTGA CCGTGCCCTT TGATCTGACC
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA AGAGCTTGGA GAAAAATGTT
    TCTCAGACAG AACATACTAC AGAACAGCTT CAGAAAGATG AGCAGGAGAC ATCCTCTCCT
    CTAGAATCAG GACCACAGCC TCATGAGGCC TCATCTGGGC TTCATGAAAA AGAGGACAAA
    GAAGAATCAA AGGCAATCCC AAAGTGGTCA TCACGAGCAC AGCACGGTGC AGCGGTAGAG
    AAAGTTCTGA GGACAGGCCA AGCTGTTCCT GATCAGCTTC TGGTGGACAT TATTGTGGAT
    GCTATCAGGA ACATTCCTGC AGATAGTGGC TGGATTCTTG ATGGCTTCCC GGTGGACATC
    AGTCAGGCTC AGATGCTTGA GAAAGCTCTA AACATGACTG AACTTGATGG AACAAAAACA
    CAAAATAATT TGTCCACAAA CAAAAACGGC CCTAAAGATG CGGCTCCTCC ATCCCCTGCT
    CTAGACCTAG TGGTGCTGTT AGACGTTTCA GATGAGCAGG TTCTGGAGCG AGCCACAACT
    CAGCCTCCTG AGGGAACACA GATAGTGAAT GGAGAAAGCA TAAAGCAGGA GAAAGGCAGC
    AGTGATGCTG CGGAGGAAAA CACAAGCATG CCAGATCTGA ACACACAAGA TCCTGAATCC
    TTGTCTGACG ACAAAAGCCT GGGGAGGACA CAAATACAGT TCAGAATCAG TGGCTTTCAT
    CAAACATGGC CAAAACTGGA GAAGTGGTTT GGTGATCAGC AGAATATTCT GGTGAAAGTC
    ACAGCAGGTG TGGACAAGGA CACTCTCTTC AGTAACGTGG AGACTATTCT GAGGGACACT
    ATTGAGTCTG TTGAAAAAGC TCTGGCTGAC TCCAGGAGAG CTTCAGTGAC CTCCACACCT
    GAACAGACCC GTCCAGAAAG TGCCTGCTCT TCCAAATCTT CCCGAGCTAC CCCAAAATCT
    GCTAGAAGAA GCTTTGCGGA GGAACCTTTA CCCAAGGAGT TGGCAGAATA TCTTGTGCCA
    TACTGGGAGA ACTTCTGTAG TTCATATGTG ACTAATGTCA AAACAGTGAT GCAGAATCTC
    CGTGGTGAAC GTGAGCTCAT CATTCATCAC CTTTACAACA TCCGGGAGAA CTTCAGGCAG
    TATCTACAGA AGCCGGATCT GAAGCAGGAG TTTGTTAGCT CATGGCAGCG AGATTATAAC
    AGTGTTCCTG ATAACATCAG AGAGGACGAG GAGACCAAAG GAGAGCTTCA TCAAAGGCTG
    GATGACTTGC GTGAGCGGCT ATGGGACATC AGTGATAAGC GTAAAGACGA GGCAGAGCAG
    GAGAAAGCAG GCGTCATTCA TGATGGCTGG TTAGAGGATC ACACCGCTGT ACTAATCAAC
    AATTATTCTG CATTGATGCA GATAGAAGTG GGCCGATTTC AGGACTGTGT GTGTCTGCTG
    AAAGATTATT ATTCAACGAT GGGTAAAACA GCCTTTGAAT CCAAATGTGA CTTTGCTCGT
    GTCCCATTGC TTGACATCAC CGCTGATCAG CATGTCGACG AGTCGAAAAT CTCTGAATCT
    GCTCCGTCAG AGAAATCTGT AAAGAATGCT GAGGAAAAAG ACTCTGAAAT TGTGGACAGG
    ATGAAAACTA AAGTGTTTCC ACTGGTTTCC TGCAGATCTC CAACTTCTGA GATCCTCAAA
    CAGCCTTTGC ATCAGGCTGA TGAGAACCTG CTGCAGGAGT TCTTCCAAGC AGCAATCGCC
    ATCTTCAGGA GCATGGTGTC GGCAGAGACG CGGCAGTGGG ATGAAGAAGA AGTTGATGAA
    GACCAGAAGC AGATGGAGGC ACAGAAAGCT CAAACTTTGA CCTCTGCTAC AGACGTAAGG
    AAAGCAGGAA AACAAAAAGG TGCTCCACCT CCTCCTCCTC CAGAGCCCAG TCCTCAACCT
    GCTGCAAGGA ACCCAGAGGA GCTCAGGAAG AGAGCGGAGA AGAAACGATT CAAACAGGAG
    CTCTCAGCTG CACTTAAACC TGAAGAAAAT GCAGTGAAGC AGCGTTTGGA GCTAGTGAAG
    GCTCATGGGC TGAGAACAGT CATCAGTCTG CAGCACAAAG CAGAACAGGT CTATAGGAAC
    ATGGAGGAGT GGATGAAAGC CCGCTATCTG GCTGAAATGA AAAGTATCGA CCAGCTGACA
    GATTTGGTGA GGCAGCACAT TGAGACTGGA GTTCAGATCC GTCATGAGCT GGTTCTGCAG
    AGCACTGATT TCTGTGTGGA TGGAGACACC CGTGTGCTGT TGAGTCCAGA GCTTACTCCG
    CGCCGATCCC TGCTGGAGCA GCCTAAAAAC AGCACACTCA CAGTACAACA GCTGCACGTC
    CTCTGCACAC AGTTGCGCAA GATCGCCCCT AAAGGTCTGA TGTGTAGAAG TGAGCTGACT
    GAAGCTCTAC ACGAATTAAC CTCTGCTCAC ATGGGTACTG ATGTCCTTCC TGAGGCCTGG
    ATGCACATCA CTCCTTCACA GGTTGAGGAG CTGGTGTGTA TTCTAGCTCA GGACTCTGAG
    ATGTTGGACT GGCGTCAGTT TCTGCTCAGT GCTGCTCTGC CGTGGCCATT TCCCACTCAG
    ACTCAGCTGA TAAAAACCCT CCAGCGCTAC AGAGACATCG ACACCGCGGA AACCGGACTC
    ATCACACAAG AACAATATGA ACAGGTTGAA TTATGGTTTC CCAGTGAACA AGATCCTTCT
    GTCCCTGATG ACCCAACTGA ACCCGTGCCC TATGACAGAT TGGCCAACCT TAAAAAGTTT
    TTCTTCAGTC TGTTTGCGAG CACACACTCC TCACCACTGC TGCTGGACTA TCTGATGATG
    CTGCTTCATT TCTGCTGCCA TCCTGAAGCA GCTCAGGGCT TCACCAGAGC GCTCGGCCTC
    GTCACCGGAC ACACACTGCA CTACAAACAC ACCACCCCAC TCACACAGTC TGTGCCGTGT
    CTGGAGGGCG CTGGAGTTGT GGATGATGCT GGTGCTGATA ATGAAGAGGA GCAGCACAGA
    GGGGTGGAGT CTGAGGAAGT GGGCGTGTCT GTTGATGATG TGCTCAGAGT TTTGAGTCAT
    GGAGAGGATC TCGTCTTACA TCTAAACCGC TTCCAGCTTG TCCACAGGAG CAGAGATGAA
    CTCCGAGAGG AGCTTCTGAA GGTGTTTAAG GACTTGGGTT TCAGTGGTGA AGAGAAGATT
    CCTTTCTCCA CACTATCTCA GCATCCCTTT CTGCAGGACC TGATAGAAGG ATCCGCCTAT
    TATCTGCTCC ATGACATTCA CAAAATTTTC CAGACAAAAC AAACTGAAGA TTCGAGAAGT
    TTCTCAACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41927
    Clone ID Related Accession (Same CDS sequence) XM_009301184.2 , XM_009301184.3
    Accession Version XM_009301184.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4878bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product sperm flagellar protein 2 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301184.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC 
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA AGAGCTTGGA GAAAAATGTT
    TCTCAGACAG AACATACTAC AGAACAGCTT CAGAAAGATG AGCAGGAGAC ATCCTCTCCT
    CTAGAATCAG GACCACAGCC TCATGAGGCC TCATCTGGGC TTCATGAAAA AGAGGACAAA
    GAAGAATCAA AGGCAATCCC AAAGTGGTCA TCACGAGCAC AGCACGGTGC AGCGGTAGAG
    AAAGTTCTGA GGACAGGCCA AGCTGTTCCT GATCAGCTTC TGGTGGACAT TATTGTGGAT
    GCTATCAGGA ACATTCCTGC AGATAGTGGC TGGATTCTTG ATGGCTTCCC GGTGGACATC
    AGTCAGGCTC AGATGCTTGA GAAAGCTCTA AACATGACTG AACTTGATGG AACAAAAACA
    CAAAATAATT TGTCCACAAA CAAAAACGGC CCTAAAGATG CGGCTCCTCC ATCCCCTGCT
    CTAGACCTAG TGGTGCTGTT AGACGTTTCA GATGAGCAGG TTCTGGAGCG AGCCACAACT
    CAGCCTCCTG AGGGAACACA GATAGTGAAT GGAGAAAGCA TAAAGCAGGA GAAAGGCAGC
    AGTGATGCTG CGGAGGAAAA CACAAGCATG CCAGATCTGA ACACACAAGA TCCTGAATCC
    TTGTCTGACG ACAAAAGCCT GGGGAGGACA CAAATACAGT TCAGAATCAG TGGCTTTCAT
    CAAACATGGC CAAAACTGGA GAAGTGGTTT GGTGATCAGC AGAATATTCT GGTGAAAGTC
    ACAGCAGGTG TGGACAAGGA CACTCTCTTC AGTAACGTGG AGACTATTCT GAGGGACACT
    ATTGAGTCTG TTGAAAAAGG AGCAGCTCTG GCTGACTCCA GGAGAGCTTC AGTGACCTCC
    ACACCTGAAC AGACCCGTCC AGAAAGTGCC TGCTCTTCCA AATCTTCCCG AGCTACCCCA
    AAATCTGCTA GAAGAAGCTT TGCGGAGGAA CCTTTACCCA AGGAGTTGGC AGAATATCTT
    GTGCCATACT GGGAGAACTT CTGTAGTTCA TATGTGACTA ATGTCAAAAC AGTGATGCAG
    AATCTCCGTG GTGAACGTGA GCTCATCATT CATCACCTTT ACAACATCCG GGAGAACTTC
    AGGCAGTATC TACAGAAGCC GGATCTGAAG CAGGAGTTTG TTAGCTCATG GCAGCGAGAT
    TATAACAGTG TTCCTGATAA CATCAGAGAG GACGAGGAGA CCAAAGGAGA GCTTCATCAA
    AGGCTGGATG ACTTGCGTGA GCGGCTATGG GACATCAGTG ATAAGCGTAA AGACGAGGCA
    GAGCAGGAGA AAGCAGGCGT CATTCATGAT GGCTGGTTAG AGGATCACAC CGCTGTACTA
    ATCAACAATT ATTCTGCATT GATGCAGATA GAAGTGGGCC GATTTCAGGA CTGTGTGTGT
    CTGCTGAAAG ATTATTATTC AACGATGGGT AAAACAGCCT TTGAATCCAA ATGTGACTTT
    GCTCGTGTCC CATTGCTTGA CATCACCGCT GATCAGCATG TCGACGAGTC GAAAATCTCT
    GAATCTGCTC CGTCAGAGAA ATCTGTAAAG AATGCTGAGG AAAAAGACTC TGAAATTGTG
    GACAGGATGA AAACTAAAGT GTTTCCACTG GTTTCCTGCA GATCTCCAAC TTCTGAGATC
    CTCAAACAGC CTTTGCATCA GGCTGATGAG AACCTGCTGC AGGAGTTCTT CCAAGCAGCA
    ATCGCCATCT TCAGGAGCAT GGTGTCGGCA GAGACGCGGC AGTGGGATGA AGAAGAAGTT
    GATGAAGACC AGAAGCAGAT GGAGGCACAG AAAGCTCAAA CTTTGACCTC TGCTACAGAC
    GTAAGGAAAG CAGGAAAACA AAAAGGTGCT CCACCTCCTC CTCCTCCAGA GCCCAGTCCT
    CAACCTGCTG CAAGGAACCC AGAGGAGCTC AGGAAGAGAG CGGAGAAGAA ACGATTCAAA
    CAGGAGCTCT CAGCTGCACT TAAACCTGAA GAAAATGCAG TGAAGCAGCG TTTGGAGCTA
    GTGAAGGCTC ATGGGCTGAG AACAGTCATC AGTCTGCAGC ACAAAGCAGA ACAGGTCTAT
    AGGAACATGG AGGAGTGGAT GAAAGCCCGC TATCTGGCTG AAATGAAAAG TATCGACCAG
    CTGACAGATT TGGTGAGGCA GCACATTGAG ACTGGAGTTC AGATCCGTCA TGAGCTGGTT
    CTGCAGAGCA CTGATTTCTG TGTGGATGGA GACACCCGTG TGCTGTTGAG TCCAGAGCTT
    ACTCCGCGCC GATCCCTGCT GGAGCAGCCT AAAAACAGCA CACTCACAGT ACAACAGCTG
    CACGTCCTCT GCACACAGTT GCGCAAGATC GCCCCTAAAG GTCTGATGTG TAGAAGTGAG
    CTGACTGAAG CTCTACACGA ATTAACCTCT GCTCACATGG GTACTGATGT CCTTCCTGAG
    GCCTGGATGC ACATCACTCC TTCACAGGTT GAGGAGCTGG TGTGTATTCT AGCTCAGGAC
    TCTGAGATGT TGGACTGGCG TCAGTTTCTG CTCAGTGCTG CTCTGCCGTG GCCATTTCCC
    ACTCAGACTC AGCTGATAAA AACCCTCCAG CGCTACAGAG ACATCGACAC CGCGGAAACC
    GGACTCATCA CACAAGAACA ATATGAACAG GTTGAATTAT GGTTTCCCAG TGAACAAGAT
    CCTTCTGTCC CTGATGACCC AACTGAACCC GTGCCCTATG ACAGATTGGC CAACCTTAAA
    AAGTTTTTCT TCAGTCTGTT TGCGAGCACA CACTCCTCAC CACTGCTGCT GGACTATCTG
    ATGATGCTGC TTCATTTCTG CTGCCATCCT GAAGCAGCTC AGGGCTTCAC CAGAGCGCTC
    GGCCTCGTCA CCGGACACAC ACTGCACTAC AAACACACCA CCCCACTCAC ACAGTCTGTG
    CCGTGTCTGG AGGGCGCTGG AGTTGTGGAT GATGCTGGTG CTGATAATGA AGAGGAGCAG
    CACAGAGGGG TGGAGTCTGA GGAAGTGGGC GTGTCTGTTG ATGATGTGCT CAGAGTTTTG
    AGTCATGGAG AGGATCTCGT CTTACATCTA AACCGCTTCC AGCTTGTCCA CAGGAGCAGA
    GATGAACTCC GAGAGGAGCT TCTGAAGGTG TTTAAGGACT TGGGTTTCAG TGGTGAAGAG
    AAGATTCCTT TCTCCACACT ATCTCAGCAT CCCTTTCTGC AGGACCTGAT AGAAGGATCC
    GCCTATTATC TGCTCCATGA CATTCACAAA ATTTTCCAGA CAAAACAAAC TGAAGATTCG
    AGAAGTTTCT CAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299459.1
    CDS171..5048
    Translation

    Target ORF information:

    RefSeq Version XM_009301184.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sperm flagellar 2 (spef2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301184.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC 
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA AGAGCTTGGA GAAAAATGTT
    TCTCAGACAG AACATACTAC AGAACAGCTT CAGAAAGATG AGCAGGAGAC ATCCTCTCCT
    CTAGAATCAG GACCACAGCC TCATGAGGCC TCATCTGGGC TTCATGAAAA AGAGGACAAA
    GAAGAATCAA AGGCAATCCC AAAGTGGTCA TCACGAGCAC AGCACGGTGC AGCGGTAGAG
    AAAGTTCTGA GGACAGGCCA AGCTGTTCCT GATCAGCTTC TGGTGGACAT TATTGTGGAT
    GCTATCAGGA ACATTCCTGC AGATAGTGGC TGGATTCTTG ATGGCTTCCC GGTGGACATC
    AGTCAGGCTC AGATGCTTGA GAAAGCTCTA AACATGACTG AACTTGATGG AACAAAAACA
    CAAAATAATT TGTCCACAAA CAAAAACGGC CCTAAAGATG CGGCTCCTCC ATCCCCTGCT
    CTAGACCTAG TGGTGCTGTT AGACGTTTCA GATGAGCAGG TTCTGGAGCG AGCCACAACT
    CAGCCTCCTG AGGGAACACA GATAGTGAAT GGAGAAAGCA TAAAGCAGGA GAAAGGCAGC
    AGTGATGCTG CGGAGGAAAA CACAAGCATG CCAGATCTGA ACACACAAGA TCCTGAATCC
    TTGTCTGACG ACAAAAGCCT GGGGAGGACA CAAATACAGT TCAGAATCAG TGGCTTTCAT
    CAAACATGGC CAAAACTGGA GAAGTGGTTT GGTGATCAGC AGAATATTCT GGTGAAAGTC
    ACAGCAGGTG TGGACAAGGA CACTCTCTTC AGTAACGTGG AGACTATTCT GAGGGACACT
    ATTGAGTCTG TTGAAAAAGG AGCAGCTCTG GCTGACTCCA GGAGAGCTTC AGTGACCTCC
    ACACCTGAAC AGACCCGTCC AGAAAGTGCC TGCTCTTCCA AATCTTCCCG AGCTACCCCA
    AAATCTGCTA GAAGAAGCTT TGCGGAGGAA CCTTTACCCA AGGAGTTGGC AGAATATCTT
    GTGCCATACT GGGAGAACTT CTGTAGTTCA TATGTGACTA ATGTCAAAAC AGTGATGCAG
    AATCTCCGTG GTGAACGTGA GCTCATCATT CATCACCTTT ACAACATCCG GGAGAACTTC
    AGGCAGTATC TACAGAAGCC GGATCTGAAG CAGGAGTTTG TTAGCTCATG GCAGCGAGAT
    TATAACAGTG TTCCTGATAA CATCAGAGAG GACGAGGAGA CCAAAGGAGA GCTTCATCAA
    AGGCTGGATG ACTTGCGTGA GCGGCTATGG GACATCAGTG ATAAGCGTAA AGACGAGGCA
    GAGCAGGAGA AAGCAGGCGT CATTCATGAT GGCTGGTTAG AGGATCACAC CGCTGTACTA
    ATCAACAATT ATTCTGCATT GATGCAGATA GAAGTGGGCC GATTTCAGGA CTGTGTGTGT
    CTGCTGAAAG ATTATTATTC AACGATGGGT AAAACAGCCT TTGAATCCAA ATGTGACTTT
    GCTCGTGTCC CATTGCTTGA CATCACCGCT GATCAGCATG TCGACGAGTC GAAAATCTCT
    GAATCTGCTC CGTCAGAGAA ATCTGTAAAG AATGCTGAGG AAAAAGACTC TGAAATTGTG
    GACAGGATGA AAACTAAAGT GTTTCCACTG GTTTCCTGCA GATCTCCAAC TTCTGAGATC
    CTCAAACAGC CTTTGCATCA GGCTGATGAG AACCTGCTGC AGGAGTTCTT CCAAGCAGCA
    ATCGCCATCT TCAGGAGCAT GGTGTCGGCA GAGACGCGGC AGTGGGATGA AGAAGAAGTT
    GATGAAGACC AGAAGCAGAT GGAGGCACAG AAAGCTCAAA CTTTGACCTC TGCTACAGAC
    GTAAGGAAAG CAGGAAAACA AAAAGGTGCT CCACCTCCTC CTCCTCCAGA GCCCAGTCCT
    CAACCTGCTG CAAGGAACCC AGAGGAGCTC AGGAAGAGAG CGGAGAAGAA ACGATTCAAA
    CAGGAGCTCT CAGCTGCACT TAAACCTGAA GAAAATGCAG TGAAGCAGCG TTTGGAGCTA
    GTGAAGGCTC ATGGGCTGAG AACAGTCATC AGTCTGCAGC ACAAAGCAGA ACAGGTCTAT
    AGGAACATGG AGGAGTGGAT GAAAGCCCGC TATCTGGCTG AAATGAAAAG TATCGACCAG
    CTGACAGATT TGGTGAGGCA GCACATTGAG ACTGGAGTTC AGATCCGTCA TGAGCTGGTT
    CTGCAGAGCA CTGATTTCTG TGTGGATGGA GACACCCGTG TGCTGTTGAG TCCAGAGCTT
    ACTCCGCGCC GATCCCTGCT GGAGCAGCCT AAAAACAGCA CACTCACAGT ACAACAGCTG
    CACGTCCTCT GCACACAGTT GCGCAAGATC GCCCCTAAAG GTCTGATGTG TAGAAGTGAG
    CTGACTGAAG CTCTACACGA ATTAACCTCT GCTCACATGG GTACTGATGT CCTTCCTGAG
    GCCTGGATGC ACATCACTCC TTCACAGGTT GAGGAGCTGG TGTGTATTCT AGCTCAGGAC
    TCTGAGATGT TGGACTGGCG TCAGTTTCTG CTCAGTGCTG CTCTGCCGTG GCCATTTCCC
    ACTCAGACTC AGCTGATAAA AACCCTCCAG CGCTACAGAG ACATCGACAC CGCGGAAACC
    GGACTCATCA CACAAGAACA ATATGAACAG GTTGAATTAT GGTTTCCCAG TGAACAAGAT
    CCTTCTGTCC CTGATGACCC AACTGAACCC GTGCCCTATG ACAGATTGGC CAACCTTAAA
    AAGTTTTTCT TCAGTCTGTT TGCGAGCACA CACTCCTCAC CACTGCTGCT GGACTATCTG
    ATGATGCTGC TTCATTTCTG CTGCCATCCT GAAGCAGCTC AGGGCTTCAC CAGAGCGCTC
    GGCCTCGTCA CCGGACACAC ACTGCACTAC AAACACACCA CCCCACTCAC ACAGTCTGTG
    CCGTGTCTGG AGGGCGCTGG AGTTGTGGAT GATGCTGGTG CTGATAATGA AGAGGAGCAG
    CACAGAGGGG TGGAGTCTGA GGAAGTGGGC GTGTCTGTTG ATGATGTGCT CAGAGTTTTG
    AGTCATGGAG AGGATCTCGT CTTACATCTA AACCGCTTCC AGCTTGTCCA CAGGAGCAGA
    GATGAACTCC GAGAGGAGCT TCTGAAGGTG TTTAAGGACT TGGGTTTCAG TGGTGAAGAG
    AAGATTCCTT TCTCCACACT ATCTCAGCAT CCCTTTCTGC AGGACCTGAT AGAAGGATCC
    GCCTATTATC TGCTCCATGA CATTCACAAA ATTTTCCAGA CAAAACAAAC TGAAGATTCG
    AGAAGTTTCT CAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41927
    Clone ID Related Accession (Same CDS sequence) XM_009301184.2 , XM_009301184.3
    Accession Version XM_009301184.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4878bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product sperm flagellar protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301184.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC 
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA AGAGCTTGGA GAAAAATGTT
    TCTCAGACAG AACATACTAC AGAACAGCTT CAGAAAGATG AGCAGGAGAC ATCCTCTCCT
    CTAGAATCAG GACCACAGCC TCATGAGGCC TCATCTGGGC TTCATGAAAA AGAGGACAAA
    GAAGAATCAA AGGCAATCCC AAAGTGGTCA TCACGAGCAC AGCACGGTGC AGCGGTAGAG
    AAAGTTCTGA GGACAGGCCA AGCTGTTCCT GATCAGCTTC TGGTGGACAT TATTGTGGAT
    GCTATCAGGA ACATTCCTGC AGATAGTGGC TGGATTCTTG ATGGCTTCCC GGTGGACATC
    AGTCAGGCTC AGATGCTTGA GAAAGCTCTA AACATGACTG AACTTGATGG AACAAAAACA
    CAAAATAATT TGTCCACAAA CAAAAACGGC CCTAAAGATG CGGCTCCTCC ATCCCCTGCT
    CTAGACCTAG TGGTGCTGTT AGACGTTTCA GATGAGCAGG TTCTGGAGCG AGCCACAACT
    CAGCCTCCTG AGGGAACACA GATAGTGAAT GGAGAAAGCA TAAAGCAGGA GAAAGGCAGC
    AGTGATGCTG CGGAGGAAAA CACAAGCATG CCAGATCTGA ACACACAAGA TCCTGAATCC
    TTGTCTGACG ACAAAAGCCT GGGGAGGACA CAAATACAGT TCAGAATCAG TGGCTTTCAT
    CAAACATGGC CAAAACTGGA GAAGTGGTTT GGTGATCAGC AGAATATTCT GGTGAAAGTC
    ACAGCAGGTG TGGACAAGGA CACTCTCTTC AGTAACGTGG AGACTATTCT GAGGGACACT
    ATTGAGTCTG TTGAAAAAGG AGCAGCTCTG GCTGACTCCA GGAGAGCTTC AGTGACCTCC
    ACACCTGAAC AGACCCGTCC AGAAAGTGCC TGCTCTTCCA AATCTTCCCG AGCTACCCCA
    AAATCTGCTA GAAGAAGCTT TGCGGAGGAA CCTTTACCCA AGGAGTTGGC AGAATATCTT
    GTGCCATACT GGGAGAACTT CTGTAGTTCA TATGTGACTA ATGTCAAAAC AGTGATGCAG
    AATCTCCGTG GTGAACGTGA GCTCATCATT CATCACCTTT ACAACATCCG GGAGAACTTC
    AGGCAGTATC TACAGAAGCC GGATCTGAAG CAGGAGTTTG TTAGCTCATG GCAGCGAGAT
    TATAACAGTG TTCCTGATAA CATCAGAGAG GACGAGGAGA CCAAAGGAGA GCTTCATCAA
    AGGCTGGATG ACTTGCGTGA GCGGCTATGG GACATCAGTG ATAAGCGTAA AGACGAGGCA
    GAGCAGGAGA AAGCAGGCGT CATTCATGAT GGCTGGTTAG AGGATCACAC CGCTGTACTA
    ATCAACAATT ATTCTGCATT GATGCAGATA GAAGTGGGCC GATTTCAGGA CTGTGTGTGT
    CTGCTGAAAG ATTATTATTC AACGATGGGT AAAACAGCCT TTGAATCCAA ATGTGACTTT
    GCTCGTGTCC CATTGCTTGA CATCACCGCT GATCAGCATG TCGACGAGTC GAAAATCTCT
    GAATCTGCTC CGTCAGAGAA ATCTGTAAAG AATGCTGAGG AAAAAGACTC TGAAATTGTG
    GACAGGATGA AAACTAAAGT GTTTCCACTG GTTTCCTGCA GATCTCCAAC TTCTGAGATC
    CTCAAACAGC CTTTGCATCA GGCTGATGAG AACCTGCTGC AGGAGTTCTT CCAAGCAGCA
    ATCGCCATCT TCAGGAGCAT GGTGTCGGCA GAGACGCGGC AGTGGGATGA AGAAGAAGTT
    GATGAAGACC AGAAGCAGAT GGAGGCACAG AAAGCTCAAA CTTTGACCTC TGCTACAGAC
    GTAAGGAAAG CAGGAAAACA AAAAGGTGCT CCACCTCCTC CTCCTCCAGA GCCCAGTCCT
    CAACCTGCTG CAAGGAACCC AGAGGAGCTC AGGAAGAGAG CGGAGAAGAA ACGATTCAAA
    CAGGAGCTCT CAGCTGCACT TAAACCTGAA GAAAATGCAG TGAAGCAGCG TTTGGAGCTA
    GTGAAGGCTC ATGGGCTGAG AACAGTCATC AGTCTGCAGC ACAAAGCAGA ACAGGTCTAT
    AGGAACATGG AGGAGTGGAT GAAAGCCCGC TATCTGGCTG AAATGAAAAG TATCGACCAG
    CTGACAGATT TGGTGAGGCA GCACATTGAG ACTGGAGTTC AGATCCGTCA TGAGCTGGTT
    CTGCAGAGCA CTGATTTCTG TGTGGATGGA GACACCCGTG TGCTGTTGAG TCCAGAGCTT
    ACTCCGCGCC GATCCCTGCT GGAGCAGCCT AAAAACAGCA CACTCACAGT ACAACAGCTG
    CACGTCCTCT GCACACAGTT GCGCAAGATC GCCCCTAAAG GTCTGATGTG TAGAAGTGAG
    CTGACTGAAG CTCTACACGA ATTAACCTCT GCTCACATGG GTACTGATGT CCTTCCTGAG
    GCCTGGATGC ACATCACTCC TTCACAGGTT GAGGAGCTGG TGTGTATTCT AGCTCAGGAC
    TCTGAGATGT TGGACTGGCG TCAGTTTCTG CTCAGTGCTG CTCTGCCGTG GCCATTTCCC
    ACTCAGACTC AGCTGATAAA AACCCTCCAG CGCTACAGAG ACATCGACAC CGCGGAAACC
    GGACTCATCA CACAAGAACA ATATGAACAG GTTGAATTAT GGTTTCCCAG TGAACAAGAT
    CCTTCTGTCC CTGATGACCC AACTGAACCC GTGCCCTATG ACAGATTGGC CAACCTTAAA
    AAGTTTTTCT TCAGTCTGTT TGCGAGCACA CACTCCTCAC CACTGCTGCT GGACTATCTG
    ATGATGCTGC TTCATTTCTG CTGCCATCCT GAAGCAGCTC AGGGCTTCAC CAGAGCGCTC
    GGCCTCGTCA CCGGACACAC ACTGCACTAC AAACACACCA CCCCACTCAC ACAGTCTGTG
    CCGTGTCTGG AGGGCGCTGG AGTTGTGGAT GATGCTGGTG CTGATAATGA AGAGGAGCAG
    CACAGAGGGG TGGAGTCTGA GGAAGTGGGC GTGTCTGTTG ATGATGTGCT CAGAGTTTTG
    AGTCATGGAG AGGATCTCGT CTTACATCTA AACCGCTTCC AGCTTGTCCA CAGGAGCAGA
    GATGAACTCC GAGAGGAGCT TCTGAAGGTG TTTAAGGACT TGGGTTTCAG TGGTGAAGAG
    AAGATTCCTT TCTCCACACT ATCTCAGCAT CCCTTTCTGC AGGACCTGAT AGAAGGATCC
    GCCTATTATC TGCTCCATGA CATTCACAAA ATTTTCCAGA CAAAACAAAC TGAAGATTCG
    AGAAGTTTCT CAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299459.1
    CDS202..5079
    Translation

    Target ORF information:

    RefSeq Version XM_009301184.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sperm flagellar 2 (spef2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301184.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC 
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA AGAGCTTGGA GAAAAATGTT
    TCTCAGACAG AACATACTAC AGAACAGCTT CAGAAAGATG AGCAGGAGAC ATCCTCTCCT
    CTAGAATCAG GACCACAGCC TCATGAGGCC TCATCTGGGC TTCATGAAAA AGAGGACAAA
    GAAGAATCAA AGGCAATCCC AAAGTGGTCA TCACGAGCAC AGCACGGTGC AGCGGTAGAG
    AAAGTTCTGA GGACAGGCCA AGCTGTTCCT GATCAGCTTC TGGTGGACAT TATTGTGGAT
    GCTATCAGGA ACATTCCTGC AGATAGTGGC TGGATTCTTG ATGGCTTCCC GGTGGACATC
    AGTCAGGCTC AGATGCTTGA GAAAGCTCTA AACATGACTG AACTTGATGG AACAAAAACA
    CAAAATAATT TGTCCACAAA CAAAAACGGC CCTAAAGATG CGGCTCCTCC ATCCCCTGCT
    CTAGACCTAG TGGTGCTGTT AGACGTTTCA GATGAGCAGG TTCTGGAGCG AGCCACAACT
    CAGCCTCCTG AGGGAACACA GATAGTGAAT GGAGAAAGCA TAAAGCAGGA GAAAGGCAGC
    AGTGATGCTG CGGAGGAAAA CACAAGCATG CCAGATCTGA ACACACAAGA TCCTGAATCC
    TTGTCTGACG ACAAAAGCCT GGGGAGGACA CAAATACAGT TCAGAATCAG TGGCTTTCAT
    CAAACATGGC CAAAACTGGA GAAGTGGTTT GGTGATCAGC AGAATATTCT GGTGAAAGTC
    ACAGCAGGTG TGGACAAGGA CACTCTCTTC AGTAACGTGG AGACTATTCT GAGGGACACT
    ATTGAGTCTG TTGAAAAAGG AGCAGCTCTG GCTGACTCCA GGAGAGCTTC AGTGACCTCC
    ACACCTGAAC AGACCCGTCC AGAAAGTGCC TGCTCTTCCA AATCTTCCCG AGCTACCCCA
    AAATCTGCTA GAAGAAGCTT TGCGGAGGAA CCTTTACCCA AGGAGTTGGC AGAATATCTT
    GTGCCATACT GGGAGAACTT CTGTAGTTCA TATGTGACTA ATGTCAAAAC AGTGATGCAG
    AATCTCCGTG GTGAACGTGA GCTCATCATT CATCACCTTT ACAACATCCG GGAGAACTTC
    AGGCAGTATC TACAGAAGCC GGATCTGAAG CAGGAGTTTG TTAGCTCATG GCAGCGAGAT
    TATAACAGTG TTCCTGATAA CATCAGAGAG GACGAGGAGA CCAAAGGAGA GCTTCATCAA
    AGGCTGGATG ACTTGCGTGA GCGGCTATGG GACATCAGTG ATAAGCGTAA AGACGAGGCA
    GAGCAGGAGA AAGCAGGCGT CATTCATGAT GGCTGGTTAG AGGATCACAC CGCTGTACTA
    ATCAACAATT ATTCTGCATT GATGCAGATA GAAGTGGGCC GATTTCAGGA CTGTGTGTGT
    CTGCTGAAAG ATTATTATTC AACGATGGGT AAAACAGCCT TTGAATCCAA ATGTGACTTT
    GCTCGTGTCC CATTGCTTGA CATCACCGCT GATCAGCATG TCGACGAGTC GAAAATCTCT
    GAATCTGCTC CGTCAGAGAA ATCTGTAAAG AATGCTGAGG AAAAAGACTC TGAAATTGTG
    GACAGGATGA AAACTAAAGT GTTTCCACTG GTTTCCTGCA GATCTCCAAC TTCTGAGATC
    CTCAAACAGC CTTTGCATCA GGCTGATGAG AACCTGCTGC AGGAGTTCTT CCAAGCAGCA
    ATCGCCATCT TCAGGAGCAT GGTGTCGGCA GAGACGCGGC AGTGGGATGA AGAAGAAGTT
    GATGAAGACC AGAAGCAGAT GGAGGCACAG AAAGCTCAAA CTTTGACCTC TGCTACAGAC
    GTAAGGAAAG CAGGAAAACA AAAAGGTGCT CCACCTCCTC CTCCTCCAGA GCCCAGTCCT
    CAACCTGCTG CAAGGAACCC AGAGGAGCTC AGGAAGAGAG CGGAGAAGAA ACGATTCAAA
    CAGGAGCTCT CAGCTGCACT TAAACCTGAA GAAAATGCAG TGAAGCAGCG TTTGGAGCTA
    GTGAAGGCTC ATGGGCTGAG AACAGTCATC AGTCTGCAGC ACAAAGCAGA ACAGGTCTAT
    AGGAACATGG AGGAGTGGAT GAAAGCCCGC TATCTGGCTG AAATGAAAAG TATCGACCAG
    CTGACAGATT TGGTGAGGCA GCACATTGAG ACTGGAGTTC AGATCCGTCA TGAGCTGGTT
    CTGCAGAGCA CTGATTTCTG TGTGGATGGA GACACCCGTG TGCTGTTGAG TCCAGAGCTT
    ACTCCGCGCC GATCCCTGCT GGAGCAGCCT AAAAACAGCA CACTCACAGT ACAACAGCTG
    CACGTCCTCT GCACACAGTT GCGCAAGATC GCCCCTAAAG GTCTGATGTG TAGAAGTGAG
    CTGACTGAAG CTCTACACGA ATTAACCTCT GCTCACATGG GTACTGATGT CCTTCCTGAG
    GCCTGGATGC ACATCACTCC TTCACAGGTT GAGGAGCTGG TGTGTATTCT AGCTCAGGAC
    TCTGAGATGT TGGACTGGCG TCAGTTTCTG CTCAGTGCTG CTCTGCCGTG GCCATTTCCC
    ACTCAGACTC AGCTGATAAA AACCCTCCAG CGCTACAGAG ACATCGACAC CGCGGAAACC
    GGACTCATCA CACAAGAACA ATATGAACAG GTTGAATTAT GGTTTCCCAG TGAACAAGAT
    CCTTCTGTCC CTGATGACCC AACTGAACCC GTGCCCTATG ACAGATTGGC CAACCTTAAA
    AAGTTTTTCT TCAGTCTGTT TGCGAGCACA CACTCCTCAC CACTGCTGCT GGACTATCTG
    ATGATGCTGC TTCATTTCTG CTGCCATCCT GAAGCAGCTC AGGGCTTCAC CAGAGCGCTC
    GGCCTCGTCA CCGGACACAC ACTGCACTAC AAACACACCA CCCCACTCAC ACAGTCTGTG
    CCGTGTCTGG AGGGCGCTGG AGTTGTGGAT GATGCTGGTG CTGATAATGA AGAGGAGCAG
    CACAGAGGGG TGGAGTCTGA GGAAGTGGGC GTGTCTGTTG ATGATGTGCT CAGAGTTTTG
    AGTCATGGAG AGGATCTCGT CTTACATCTA AACCGCTTCC AGCTTGTCCA CAGGAGCAGA
    GATGAACTCC GAGAGGAGCT TCTGAAGGTG TTTAAGGACT TGGGTTTCAG TGGTGAAGAG
    AAGATTCCTT TCTCCACACT ATCTCAGCAT CCCTTTCTGC AGGACCTGAT AGAAGGATCC
    GCCTATTATC TGCTCCATGA CATTCACAAA ATTTTCCAGA CAAAACAAAC TGAAGATTCG
    AGAAGTTTCT CAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.