plxnd1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	plxnd1
Entrez Gene ID	402998
Full Name	plexin D1
Synonyms	zgc:77379
General protein information	Preferred Names plexin D1 Names plexin-D1 obd out of bounds
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_205697.2	NP_991260.2	plexin-D1 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Plxnd1	NP_080652.2
Gallus gallus (chicken)	PLXND1	XP_414455.3
Xenopus tropicalis (tropical clawed frog)	plxnd1	XP_002936302.2
Homo sapiens (human)	PLXND1	NP_055918.2
Canis lupus familiaris (dog)	PLXND1	XP_533732.2
Bos taurus (cattle)	PLXND1	XP_002697168.3
Rattus norvegicus (Norway rat)	Plxnd1	NP_001101351.1
Danio rerio (zebrafish)	plxnd1	NP_991260.2

Gene Ontology
Bibliography

Related articles in PubMed

Neuronal sFlt1 and Vegfaa determine venous sprouting and spinal cord vascularization.
Wild R, Klems A, Takamiya M, Hayashi Y, Str?hle U, Ando K, Mochizuki N, van Impel A, Schulte-Merker S, Krueger J, Preau L, le Noble F
Nature communications813991(2017 Jan)

Sema3d controls collective endothelial cell migration by distinct mechanisms via Nrp1 and PlxnD1.
Hamm MJ, Kirchmaier BC, Herzog W
The Journal of cell biology215(3)415-430(2016 Nov)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sema3d regulates collective endothelial cell migration in zebrafish through two separate mechanisms. Mesenchymal Sema3d guides outgrowth of the common cardinal vein via repulsion and signals through PlexinD1.
Title: Sema3d controls collective endothelial cell migration by distinct mechanisms via Nrp1 and PlxnD1.

Plxnd1 is a novel regulator of VAT growth, body fat distribution, and insulin sensitivity in both zebrafish and humans
Title: Plexin D1 determines body fat distribution by regulating the type V collagen microenvironment in visceral adipose tissue.

Somite expression of known vascular guidance cues, efnb2, sema3a2, and plexinD1 are disrupted, suggesting that the inter-somitic vessel vascular phenotype is due to disruption of these cues.
Title: Zebrafish notch signalling pathway mutants exhibit trunk vessel patterning anomalies that are secondary to somite misregulation.

Loss of plxnB2 results in delayed ISV sprouting identical to that seen in sema3e morphants, while loss of plexinD1 in out of bounds (obd) mutants results in precocious ISV sprouting.
Title: Antagonistic interactions among Plexins regulate the timing of intersegmental vessel formation.

We show that proper blood vessel pathfinding requires the endothelial receptor PlexinD1 and semaphorin signals, and we identify mutations in plexinD1 in the zebrafish vascular patterning mutant out of bounds
Title: Semaphorin-plexin signaling guides patterning of the developing vasculature.

The following plxnd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the plxnd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35709	NM_205697.2 Latest version!	Danio rerio plexin D1 (plxnd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

plxnd1 ( NM_205697.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_991260.2 Danio rerio plexin D1 (plxnd1), mRNA.

Price & Availability↑

CloneID	ODa35709
Clone ID Related Accession (Same CDS sequence)	NM_205697.2
Accession Version	NM_205697.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-02
Organism	Danio rerio(zebrafish)
Product	plexin-D1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY648302.1. On Sep 28, 2011 this sequence version replaced NM_205697.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY648302.1 [ECO:0000332] ##Evidence-Data-END##

ATGCCTCCGT CAGCAGCGGA CCGACGCTCG GCGCTCGCGC TGCTCTCCGC GCTGCTCTTA 
GCGGCTCTCC AGACGCGCTC GGCCGGCGCG CTGCACGTTC AACAGGCGTT CGCGACTCCA 
GGACGCACCA ATAACTTCGC GCTGGACGCG GCGTCAGGAC GCGTCTACCT GGCCGCCGTT 
AACAACCTGT ACCAGCTGAA CGCGACGCTG GCGCTGGAGG TGGAGATGCG CACGGGACCC 
GTGCTGGACA ACCCGCTGTG CCACGCGCCA CAGCTGCCGC AGGCGACCTG CGAGCACCAG 
AAGACGCTCA CCGACAACCA CAACAAGCTG CTGGCGCTGG ACCGCGCGCA GGACGTGCTG 
CTGGCGTGCG GATCCGTGTA CCAGGGCTTC TGCGAGCTGC GGCGGCTGGA GAACGTGTCC 
CGGCTGGCGG TGCAGTTCCC GCAGGACGGC GCCACCGTGT TCCCCAGCAT GCTCAACATC 
GCCGCCAACC ACGAGAACGC GAGCACCGTC GGCCTGGTGT TCCGCACTCA CGGCGGCTCC 
CCGCGGCTGC TGGTGGGCGC CACATACACC GGCATGGGCA CCGAGTACTT CCCCAAGAAC 
CACAGCAAAG AGGACCTGCG CTTCGAGAAC ACCCCGGAGA TCGCCATCCG CGCGCTGGAC 
ACGCGCGAGC TCGGCCGCCT CTTCACCTAC GACATCAACC CGTCCGAGGA CAACGTCTTC 
AAGATCAAGC AGGAGGTCAA GCAGAAGAAC AAGCTGAGCT TCGTGCACGC GTTCGCGCTC 
GGGAACTACT CCTACATCGC CTTCAACAAC GACGCGAACA GCGGGCTGAA GGAGAGCCAG 
CCGAACAGCG TGCTGGCGCG CATCTGCCTG GACACCGACG CGCCGCGCAG AGCCGCCGAG 
AGCCGCAAGC TAACCGAGTC GTACGCGCAG ATGGGGCTCC GGTGCGGCAC CTACACCCGC 
CTGCTGTCCG TCAGCCCGGC GGAGCTGCGC GCGGAGACGT TCCTGTTCGC GGTGTTCGGG 
CGCGCGGACG GGCGCGCGGC GGTGTGCGTG TTCCGCGTGG CCGAGGTGGA GGAGATGATC 
CGGCAGGGCC GCAGGAACTG CTCTCACGGG CCGAACAGCG ATGTGCAGGT GCTGGACAGC 
GTCATCCAGG GCTCCGGGGC CGAGTGCGAG GGCAAGGGCA GCATCATGCT GCAGCTGCAG 
ACGGATCAGC TGAACTGTGG AGCCGCTCAC CTGCAGCACC CGCTAGCCCT GCGGAGACCC 
CTGAGGGCCC GACCGCTGTA TGAGGCCCAG GGGCTGAGCT CTGTGGCGGT CGACAGCGCA 
CACAACCACA CACTCATGTT CCTGGGCAGC AGAGGACGAC TGCACAAGGT CTCTCTGCAC 
TCCAACTTCA GTGTCTCCCA GCAGTGGTCA CTGAGGCTGC CCGCTAATGA ACCCGTTCAC 
CACATCATGA CCTTTGACCC CAGTGACCGC ACCTACCTGT ATGTGATGAC CTCACACCAC 
CTGCTGCGGG TCCGTGTGTC CTCCTGTGAG CAGTACAGCT CGTGTGGAGA CTGTCTCAGT 
GCTGGAGATC CTCACTGCGG ATGGTGCACA CTGGAGAGAA GGTGTTCTGT TCAGCAGGAC 
TGCTCCAGTG TGTCTCTGAG CCGCTCGTGG ATCAGCATCA GTGAAGGAGT CCAGCAGTGT 
CCATCCATGA CCATCACTCC TGCTGAGATC AGCCGCTCCG CTGAAATGAG GGATGTGGGG 
ATCCTGGTTG CCGGCAGTGT TCCGGATCTC CAGGGTCTGC GGGTGGAGTG TGATTATGGG 
ATGGGGATCT CCACCAACGC CACTGTTCAC CTGGACTACG GCACCAGCCA TATCCAGACC 
TGCCCTCTGC CTCCAGCACA CACACTTCCC ACAATCCCCT CCGGCACAGA TCACGTCACT 
GTTCCTGTGT CCATCAACGC CAACGGTGTG TCTGTGGTGT CTGGAAGATT CATCATCTAC 
GACTGTGAGC GAACTGGACA GATACACTCC ACAACTGCGT GCACTAGTTG TCTGAGTTCA 
GCCTGGCGGT GTTTCTGGGA CCCGCAGCTC CATCAGTGTG TGTCCAGCAA GAACAACACA 
CAGCAGCTTC TGCAGAATTC CTCTTCCTGT CCCAGTATGG TGGCCTATGA GGTTCCTCCG 
CTGCCCACAG GAAGCTCTCC TGTTTTCTCC CTGGAGCTGA AGAACGTGGA GCATGGGACA 
GCTCTGGAGT GTGTCTACAG TGACGGGCAG CAGTACAGAG CCCAGTGGCT GGACGGGCCG 
TGGGTCAACT GCAGCGGAGC GACTCTGAAA ACTACACAGT GGAGCGAGAG CTTCAGTCTG 
AACCTGCGCA GAGCTGGAGA CGCCACATAC ATCGACAATC CACAGCGCAT GACAGTGGAG 
GTGTACAGCT GCTCGGCGGG CGTCTCTGAC TGTTCTCAGT GTTTGGGTCG TGCAGCGCTC 
GGTCAGGAGT GTGTGTGGTG TGAACACATC TGCCGCCAGC AGGACCAGTG CCCGACCACC 
AGCTCTGCCC GCTGCCCAGC GCCGCTCATA CACAAGATTG AGCCACTGTC GGGGCCTCTG 
ACCGGAGGAA CGCTCCTGAC GGTGACGGGC AAGAATCTGG GTCACCGTGC GGATCAGCTC 
AGCGTGTCCA TCGGCGGCGT AACCTGCCAC ACACTCCCAC AGCAATACAC CGTCTCCATC 
AGGCTGGTTT GCGAGACTGA TGCATCATTG GAGCAGATGA GTGGTCAAGT GACGGTGGGC 
GTGGCCGGGG ATGCAGTGGG CGTGTCTAAG GAGCAGTTCT CCTTTGTGGA GCCTCGACTG 
CTGCGGTTCA GCCCGGCTCA GGGGCCTCTC GCTGGAGGAA CCACACTCAC CATCCAGGGC 
CAGTTCCTGG ACGCCGGCTC CACCACTGAC ATCCAGATCA ACAACACACA CACCTGCTCC 
ATCCGCACGC GGTCAAGTGA GATAATCAGG TGTGTGATGC CGGCAGCTGC GAGCGCAGAG 
AATGTGTCGG TGTGTGTGGT GTTTGACGGC CGGCCGTGTG TGAGCGCTTC ACCAAACTTC 
ACATTCAGCT ACCAGAAAAA CCCCACCATC AGCCACATCT GGCCCAGCAG GAGCTACATC 
AGTGGTGGTC GCAGTATCTC CGTCTCTGGC GTGGGCTTTG ACCTGGTTCA GTCAGTGGTG 
ATGGAGGTTC CTGGAGTCGG GCAGACGAAC TGCTCGTGTG TGTCGTCCAC ACTGATCCTC 
TGTCAGTCTC CGGCGGCGGA TCAAGCGCAG CAGACCGCAG TGCTCTTCTC CTTGAATGGT 
GTGCAGTACC GAGACGCTGC CTCCGCAGGC CTGGAGGAGG GCGAGCAGCC GCACACACAG 
CGATACAGCT TCCAGTATGT GGAGGACCCG CAGTTCTACA CCGCTAACAA GGAGAAACTG 
ATCAAACACC ATCCAGGAGA ACCACTCATA CTCATCATCA ACAAAGGACC AAGTGATCTG 
GACCTGACAC TGGATGAATA CAGCGTGACG ATTGGCTCAG ACCTCTGTGA CATCACCTTC 
CATAACCAGC AGCTGTTCCA CTGCAGCATC AACAGATCAC TGAGTGCCAG CACTGGAGAG 
CTGCCCGTCA CTGTGCGCGT CGGTCACTTC CAGAAGGTGA TTGCGATGGT GCAGATGGGT 
GGAGGCAGTG AACTGGCCAT AGTGGTGTCC ATAGTGGTGT GCTGTGTACT GCTGCTGCTC 
TGCACTGTGG CACTGGTGGT GTACTGCACT AAGAGCCGCA GGGCTGAGCG CTACTGGCAG 
AAGACTCTGG TGCAGATGGA GGAGATGGAG TCACAGATCC GCGACGAGAT ACGCAAAGGT 
TTTGCGGAGC TGCAGACAGA TATGACGGAT CTGACCAAAG AGCTGAGCCG CACACAGGGA 
ATCCCCTTCC TGGAGTATAA GCAGTTCGTC ACGCGCACCT TCTTCCCCAA GATGTGCTGT 
GACTATGAGA GGCGTTTGGT TCAGCCGGTT TATGAGAACG ATCCTCTGGG TCCGAGAGCT 
CACTCTGAAA CACACCCACT GCTGCAGGAC TGGCAGCCGG CGGGCAGCGT TCGACCCAAT 
CTGGAGGAGG GCATCACACT GTTCTCCACA CTGCTCAACA ACAAACACTT CCTGGTCACC 
TTCGTCCACG CGCTCGAGCA GCAGAGAGAC TTCGCCGTCA GAGACCGGTG TAATCTGGCG 
TCTCTGCTCA CCATCGCCCT CCATGGTAAA CTGGAGTACT ACACCAGCAT CATGAAGGAT 
CTGCTGGTGG ATCTGATCGA CGCCTCGGCC ACCAAGAACC CCAAACTGAT GCTGCGGCGC 
ACCGAGAGCG TGGTCGAGAA GATGCTCACC AACTGGATGT CCATCTGCAT GTACAGCTAC 
CTGAAGGAGA CAGTGGGCGA GCCGTTCTTC CTGCTCCTGT GTGCCATCAA ACAGCAGATC 
AACAAGGGCT CCATCGACGT GCTGACGGGG AAAGCGCGAT ACACCCTCAA CGAGGAGTGG 
CTTCTGCGCG AAAACATCGA AGCCAAACCA CAGAATGTGA TGGTGTCGTT CCAAGGCCTG 
GGCATGGACT CGGTGTGTGT GCGCGTGATG AACAGCGACA CCATCTGCCA GGTGAAGGAG 
AAGATCCTGG AGGCTTTCTA CAAGAACCTG CCCTACTCAC AGTGGCCCCG AGAGGAGGAC 
GTGGAGCTGG AGTGGTTTCC TGAAGGACGC AGCAGCAGAA TCCTGCAGGA TCTGGACGAC 
TCGTCTGTCA TGGAGGACGG CAGGAAGAAA CTCAACACTG TCTTCCACTA TCAGATCCCT 
GATGGAGCAT CACTGGCCAT GAGCCTGAAG GACAAGAGAG AAAACACACT GGGCAGAGTG 
AAAGATCTGG ACACAGAGAA GTATGTTCAT CTGGTTCTTC CTCATGATGA GCTGATGGAG 
AGCAGGAAGA CGCACCGGCA GAGTCAGCGC AAGAAGGTGC TGCCGGAGAT TTACCTGACC 
CGCCTGCTGT CCACCAAGGG CACGCTGCAG AAGTTTCTGG ATGATCTGTT TCAGGCTATC 
CTCAGTGTGC CAGCAGAGAA TCCTCCGCTC GCCATCAAGT ACTTCTTCGA CTTCCTGGAG 
GAGCAGGCGG ACAAACGCGG CATCACCGAC CCCGACACAC TGCACATCTG GAAGACCAAC 
AGTCTGCCGC TGCGCTTCTG GGTGAACATC TTGAAGAATC CTCAGTTTGT GTTCGACATC 
GAGAAGACGG ACCACATGGA CGCCTGCCTG TCGGTCATCG CTCAGGCCTT CATCGACGCC 
TGCTCACTGT CTGACCTGCA GCTCGGCAAG GACTCTCCCA CTAATAAGCT GCTGTACGCT 
AAAGAGATCC CGGAGTACAA GAAGAGGGTG CAGAGCTTCT ACAGACAGAT CCAGGAGCTG 
CCGGCGCTCA GCGAACAGGA GATGAACACA CACCTGGCCG AGGAGTCCCG GAAACACAGG 
AACGAGTTCA ACACTACCCT GGCCCTCACT GAGGTCTACA AGTACGCCAA GAGATACCGT 
GCTCAGGTGG CGAGTGCGCT GGACTCGAAC CCGACCGCTC GACGCACGCA GCTGCAGCAC 
AAGTTTGAGC AGGTCATCGC TCTGGTGGAG GACAACATCT ATGAGTGCAG CAGCGAGGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_991260.2
CDS	102..5744
Translation	MPPSAADRRSALALLSALLLAALQTRSAGALHVQQAFATPGRTNNFALDAASGRVYLAAVNNLYQLNATLALEVEMRTGPVLDNPLCHAPQLPQATCEHQKTLTDNHNKLLALDRAQDVLLACGSVYQGFCELRRLENVSRLAVQFPQDGATVFPSMLNIAANHENASTVGLVFRTHGGSPRLLVGATYTGMGTEYFPKNHSKEDLRFENTPEIAIRALDTRELGRLFTYDINPSEDNVFKIKQEVKQKNKLSFVHAFALGNYSYIAFNNDANSGLKESQPNSVLARICLDTDAPRRAAESRKLTESYAQMGLRCGTYTRLLSVSPAELRAETFLFAVFGRADGRAAVCVFRVAEVEEMIRQGRRNCSHGPNSDVQVLDSVIQGSGAECEGKGSIMLQLQTDQLNCGAAHLQHPLALRRPLRARPLYEAQGLSSVAVDSAHNHTLMFLGSRGRLHKVSLHSNFSVSQQWSLRLPANEPVHHIMTFDPSDRTYLYVMTSHHLLRVRVSSCEQYSSCGDCLSAGDPHCGWCTLERRCSVQQDCSSVSLSRSWISISEGVQQCPSMTITPAEISRSAEMRDVGILVAGSVPDLQGLRVECDYGMGISTNATVHLDYGTSHIQTCPLPPAHTLPTIPSGTDHVTVPVSINANGVSVVSGRFIIYDCERTGQIHSTTACTSCLSSAWRCFWDPQLHQCVSSKNNTQQLLQNSSSCPSMVAYEVPPLPTGSSPVFSLELKNVEHGTALECVYSDGQQYRAQWLDGPWVNCSGATLKTTQWSESFSLNLRRAGDATYIDNPQRMTVEVYSCSAGVSDCSQCLGRAALGQECVWCEHICRQQDQCPTTSSARCPAPLIHKIEPLSGPLTGGTLLTVTGKNLGHRADQLSVSIGGVTCHTLPQQYTVSIRLVCETDASLEQMSGQVTVGVAGDAVGVSKEQFSFVEPRLLRFSPAQGPLAGGTTLTIQGQFLDAGSTTDIQINNTHTCSIRTRSSEIIRCVMPAAASAENVSVCVVFDGRPCVSASPNFTFSYQKNPTISHIWPSRSYISGGRSISVSGVGFDLVQSVVMEVPGVGQTNCSCVSSTLILCQSPAADQAQQTAVLFSLNGVQYRDAASAGLEEGEQPHTQRYSFQYVEDPQFYTANKEKLIKHHPGEPLILIINKGPSDLDLTLDEYSVTIGSDLCDITFHNQQLFHCSINRSLSASTGELPVTVRVGHFQKVIAMVQMGGGSELAIVVSIVVCCVLLLLCTVALVVYCTKSRRAERYWQKTLVQMEEMESQIRDEIRKGFAELQTDMTDLTKELSRTQGIPFLEYKQFVTRTFFPKMCCDYERRLVQPVYENDPLGPRAHSETHPLLQDWQPAGSVRPNLEEGITLFSTLLNNKHFLVTFVHALEQQRDFAVRDRCNLASLLTIALHGKLEYYTSIMKDLLVDLIDASATKNPKLMLRRTESVVEKMLTNWMSICMYSYLKETVGEPFFLLLCAIKQQINKGSIDVLTGKARYTLNEEWLLRENIEAKPQNVMVSFQGLGMDSVCVRVMNSDTICQVKEKILEAFYKNLPYSQWPREEDVELEWFPEGRSSRILQDLDDSSVMEDGRKKLNTVFHYQIPDGASLAMSLKDKRENTLGRVKDLDTEKYVHLVLPHDELMESRKTHRQSQRKKVLPEIYLTRLLSTKGTLQKFLDDLFQAILSVPAENPPLAIKYFFDFLEEQADKRGITDPDTLHIWKTNSLPLRFWVNILKNPQFVFDIEKTDHMDACLSVIAQAFIDACSLSDLQLGKDSPTNKLLYAKEIPEYKKRVQSFYRQIQELPALSEQEMNTHLAEESRKHRNEFNTTLALTEVYKYAKRYRAQVASALDSNPTARRTQLQHKFEQVIALVEDNIYECSSEA

Target ORF information:

RefSeq Version	NM_205697.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio plexin D1 (plxnd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205697.2

ORF Insert Sequence:

ATGCCTCCGT CAGCAGCGGA CCGACGCTCG GCGCTCGCGC TGCTCTCCGC GCTGCTCTTA 
GCGGCTCTCC AGACGCGCTC GGCCGGCGCG CTGCACGTTC AACAGGCGTT CGCGACTCCA 
GGACGCACCA ATAACTTCGC GCTGGACGCG GCGTCAGGAC GCGTCTACCT GGCCGCCGTT 
AACAACCTGT ACCAGCTGAA CGCGACGCTG GCGCTGGAGG TGGAGATGCG CACGGGACCC 
GTGCTGGACA ACCCGCTGTG CCACGCGCCA CAGCTGCCGC AGGCGACCTG CGAGCACCAG 
AAGACGCTCA CCGACAACCA CAACAAGCTG CTGGCGCTGG ACCGCGCGCA GGACGTGCTG 
CTGGCGTGCG GATCCGTGTA CCAGGGCTTC TGCGAGCTGC GGCGGCTGGA GAACGTGTCC 
CGGCTGGCGG TGCAGTTCCC GCAGGACGGC GCCACCGTGT TCCCCAGCAT GCTCAACATC 
GCCGCCAACC ACGAGAACGC GAGCACCGTC GGCCTGGTGT TCCGCACTCA CGGCGGCTCC 
CCGCGGCTGC TGGTGGGCGC CACATACACC GGCATGGGCA CCGAGTACTT CCCCAAGAAC 
CACAGCAAAG AGGACCTGCG CTTCGAGAAC ACCCCGGAGA TCGCCATCCG CGCGCTGGAC 
ACGCGCGAGC TCGGCCGCCT CTTCACCTAC GACATCAACC CGTCCGAGGA CAACGTCTTC 
AAGATCAAGC AGGAGGTCAA GCAGAAGAAC AAGCTGAGCT TCGTGCACGC GTTCGCGCTC 
GGGAACTACT CCTACATCGC CTTCAACAAC GACGCGAACA GCGGGCTGAA GGAGAGCCAG 
CCGAACAGCG TGCTGGCGCG CATCTGCCTG GACACCGACG CGCCGCGCAG AGCCGCCGAG 
AGCCGCAAGC TAACCGAGTC GTACGCGCAG ATGGGGCTCC GGTGCGGCAC CTACACCCGC 
CTGCTGTCCG TCAGCCCGGC GGAGCTGCGC GCGGAGACGT TCCTGTTCGC GGTGTTCGGG 
CGCGCGGACG GGCGCGCGGC GGTGTGCGTG TTCCGCGTGG CCGAGGTGGA GGAGATGATC 
CGGCAGGGCC GCAGGAACTG CTCTCACGGG CCGAACAGCG ATGTGCAGGT GCTGGACAGC 
GTCATCCAGG GCTCCGGGGC CGAGTGCGAG GGCAAGGGCA GCATCATGCT GCAGCTGCAG 
ACGGATCAGC TGAACTGTGG AGCCGCTCAC CTGCAGCACC CGCTAGCCCT GCGGAGACCC 
CTGAGGGCCC GACCGCTGTA TGAGGCCCAG GGGCTGAGCT CTGTGGCGGT CGACAGCGCA 
CACAACCACA CACTCATGTT CCTGGGCAGC AGAGGACGAC TGCACAAGGT CTCTCTGCAC 
TCCAACTTCA GTGTCTCCCA GCAGTGGTCA CTGAGGCTGC CCGCTAATGA ACCCGTTCAC 
CACATCATGA CCTTTGACCC CAGTGACCGC ACCTACCTGT ATGTGATGAC CTCACACCAC 
CTGCTGCGGG TCCGTGTGTC CTCCTGTGAG CAGTACAGCT CGTGTGGAGA CTGTCTCAGT 
GCTGGAGATC CTCACTGCGG ATGGTGCACA CTGGAGAGAA GGTGTTCTGT TCAGCAGGAC 
TGCTCCAGTG TGTCTCTGAG CCGCTCGTGG ATCAGCATCA GTGAAGGAGT CCAGCAGTGT 
CCATCCATGA CCATCACTCC TGCTGAGATC AGCCGCTCCG CTGAAATGAG GGATGTGGGG 
ATCCTGGTTG CCGGCAGTGT TCCGGATCTC CAGGGTCTGC GGGTGGAGTG TGATTATGGG 
ATGGGGATCT CCACCAACGC CACTGTTCAC CTGGACTACG GCACCAGCCA TATCCAGACC 
TGCCCTCTGC CTCCAGCACA CACACTTCCC ACAATCCCCT CCGGCACAGA TCACGTCACT 
GTTCCTGTGT CCATCAACGC CAACGGTGTG TCTGTGGTGT CTGGAAGATT CATCATCTAC 
GACTGTGAGC GAACTGGACA GATACACTCC ACAACTGCGT GCACTAGTTG TCTGAGTTCA 
GCCTGGCGGT GTTTCTGGGA CCCGCAGCTC CATCAGTGTG TGTCCAGCAA GAACAACACA 
CAGCAGCTTC TGCAGAATTC CTCTTCCTGT CCCAGTATGG TGGCCTATGA GGTTCCTCCG 
CTGCCCACAG GAAGCTCTCC TGTTTTCTCC CTGGAGCTGA AGAACGTGGA GCATGGGACA 
GCTCTGGAGT GTGTCTACAG TGACGGGCAG CAGTACAGAG CCCAGTGGCT GGACGGGCCG 
TGGGTCAACT GCAGCGGAGC GACTCTGAAA ACTACACAGT GGAGCGAGAG CTTCAGTCTG 
AACCTGCGCA GAGCTGGAGA CGCCACATAC ATCGACAATC CACAGCGCAT GACAGTGGAG 
GTGTACAGCT GCTCGGCGGG CGTCTCTGAC TGTTCTCAGT GTTTGGGTCG TGCAGCGCTC 
GGTCAGGAGT GTGTGTGGTG TGAACACATC TGCCGCCAGC AGGACCAGTG CCCGACCACC 
AGCTCTGCCC GCTGCCCAGC GCCGCTCATA CACAAGATTG AGCCACTGTC GGGGCCTCTG 
ACCGGAGGAA CGCTCCTGAC GGTGACGGGC AAGAATCTGG GTCACCGTGC GGATCAGCTC 
AGCGTGTCCA TCGGCGGCGT AACCTGCCAC ACACTCCCAC AGCAATACAC CGTCTCCATC 
AGGCTGGTTT GCGAGACTGA TGCATCATTG GAGCAGATGA GTGGTCAAGT GACGGTGGGC 
GTGGCCGGGG ATGCAGTGGG CGTGTCTAAG GAGCAGTTCT CCTTTGTGGA GCCTCGACTG 
CTGCGGTTCA GCCCGGCTCA GGGGCCTCTC GCTGGAGGAA CCACACTCAC CATCCAGGGC 
CAGTTCCTGG ACGCCGGCTC CACCACTGAC ATCCAGATCA ACAACACACA CACCTGCTCC 
ATCCGCACGC GGTCAAGTGA GATAATCAGG TGTGTGATGC CGGCAGCTGC GAGCGCAGAG 
AATGTGTCGG TGTGTGTGGT GTTTGACGGC CGGCCGTGTG TGAGCGCTTC ACCAAACTTC 
ACATTCAGCT ACCAGAAAAA CCCCACCATC AGCCACATCT GGCCCAGCAG GAGCTACATC 
AGTGGTGGTC GCAGTATCTC CGTCTCTGGC GTGGGCTTTG ACCTGGTTCA GTCAGTGGTG 
ATGGAGGTTC CTGGAGTCGG GCAGACGAAC TGCTCGTGTG TGTCGTCCAC ACTGATCCTC 
TGTCAGTCTC CGGCGGCGGA TCAAGCGCAG CAGACCGCAG TGCTCTTCTC CTTGAATGGT 
GTGCAGTACC GAGACGCTGC CTCCGCAGGC CTGGAGGAGG GCGAGCAGCC GCACACACAG 
CGATACAGCT TCCAGTATGT GGAGGACCCG CAGTTCTACA CCGCTAACAA GGAGAAACTG 
ATCAAACACC ATCCAGGAGA ACCACTCATA CTCATCATCA ACAAAGGACC AAGTGATCTG 
GACCTGACAC TGGATGAATA CAGCGTGACG ATTGGCTCAG ACCTCTGTGA CATCACCTTC 
CATAACCAGC AGCTGTTCCA CTGCAGCATC AACAGATCAC TGAGTGCCAG CACTGGAGAG 
CTGCCCGTCA CTGTGCGCGT CGGTCACTTC CAGAAGGTGA TTGCGATGGT GCAGATGGGT 
GGAGGCAGTG AACTGGCCAT AGTGGTGTCC ATAGTGGTGT GCTGTGTACT GCTGCTGCTC 
TGCACTGTGG CACTGGTGGT GTACTGCACT AAGAGCCGCA GGGCTGAGCG CTACTGGCAG 
AAGACTCTGG TGCAGATGGA GGAGATGGAG TCACAGATCC GCGACGAGAT ACGCAAAGGT 
TTTGCGGAGC TGCAGACAGA TATGACGGAT CTGACCAAAG AGCTGAGCCG CACACAGGGA 
ATCCCCTTCC TGGAGTATAA GCAGTTCGTC ACGCGCACCT TCTTCCCCAA GATGTGCTGT 
GACTATGAGA GGCGTTTGGT TCAGCCGGTT TATGAGAACG ATCCTCTGGG TCCGAGAGCT 
CACTCTGAAA CACACCCACT GCTGCAGGAC TGGCAGCCGG CGGGCAGCGT TCGACCCAAT 
CTGGAGGAGG GCATCACACT GTTCTCCACA CTGCTCAACA ACAAACACTT CCTGGTCACC 
TTCGTCCACG CGCTCGAGCA GCAGAGAGAC TTCGCCGTCA GAGACCGGTG TAATCTGGCG 
TCTCTGCTCA CCATCGCCCT CCATGGTAAA CTGGAGTACT ACACCAGCAT CATGAAGGAT 
CTGCTGGTGG ATCTGATCGA CGCCTCGGCC ACCAAGAACC CCAAACTGAT GCTGCGGCGC 
ACCGAGAGCG TGGTCGAGAA GATGCTCACC AACTGGATGT CCATCTGCAT GTACAGCTAC 
CTGAAGGAGA CAGTGGGCGA GCCGTTCTTC CTGCTCCTGT GTGCCATCAA ACAGCAGATC 
AACAAGGGCT CCATCGACGT GCTGACGGGG AAAGCGCGAT ACACCCTCAA CGAGGAGTGG 
CTTCTGCGCG AAAACATCGA AGCCAAACCA CAGAATGTGA TGGTGTCGTT CCAAGGCCTG 
GGCATGGACT CGGTGTGTGT GCGCGTGATG AACAGCGACA CCATCTGCCA GGTGAAGGAG 
AAGATCCTGG AGGCTTTCTA CAAGAACCTG CCCTACTCAC AGTGGCCCCG AGAGGAGGAC 
GTGGAGCTGG AGTGGTTTCC TGAAGGACGC AGCAGCAGAA TCCTGCAGGA TCTGGACGAC 
TCGTCTGTCA TGGAGGACGG CAGGAAGAAA CTCAACACTG TCTTCCACTA TCAGATCCCT 
GATGGAGCAT CACTGGCCAT GAGCCTGAAG GACAAGAGAG AAAACACACT GGGCAGAGTG 
AAAGATCTGG ACACAGAGAA GTATGTTCAT CTGGTTCTTC CTCATGATGA GCTGATGGAG 
AGCAGGAAGA CGCACCGGCA GAGTCAGCGC AAGAAGGTGC TGCCGGAGAT TTACCTGACC 
CGCCTGCTGT CCACCAAGGG CACGCTGCAG AAGTTTCTGG ATGATCTGTT TCAGGCTATC 
CTCAGTGTGC CAGCAGAGAA TCCTCCGCTC GCCATCAAGT ACTTCTTCGA CTTCCTGGAG 
GAGCAGGCGG ACAAACGCGG CATCACCGAC CCCGACACAC TGCACATCTG GAAGACCAAC 
AGTCTGCCGC TGCGCTTCTG GGTGAACATC TTGAAGAATC CTCAGTTTGT GTTCGACATC 
GAGAAGACGG ACCACATGGA CGCCTGCCTG TCGGTCATCG CTCAGGCCTT CATCGACGCC 
TGCTCACTGT CTGACCTGCA GCTCGGCAAG GACTCTCCCA CTAATAAGCT GCTGTACGCT 
AAAGAGATCC CGGAGTACAA GAAGAGGGTG CAGAGCTTCT ACAGACAGAT CCAGGAGCTG 
CCGGCGCTCA GCGAACAGGA GATGAACACA CACCTGGCCG AGGAGTCCCG GAAACACAGG 
AACGAGTTCA ACACTACCCT GGCCCTCACT GAGGTCTACA AGTACGCCAA GAGATACCGT 
GCTCAGGTGG CGAGTGCGCT GGACTCGAAC CCGACCGCTC GACGCACGCA GCTGCAGCAC 
AAGTTTGAGC AGGTCATCGC TCTGGTGGAG GACAACATCT ATGAGTGCAG CAGCGAGGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Neuronal sFlt1 and Vegfaa determine venous sprouting and spinal cord vascularization.
Nature communications813991(2017 Jan)
Wild R,Klems A,Takamiya M,Hayashi Y,Str?hle U,Ando K,Mochizuki N,van Impel A,Schulte-Merker S,Krueger J,Preau L,le Noble F

Sema3d controls collective endothelial cell migration by distinct mechanisms via Nrp1 and PlxnD1.
The Journal of cell biology215(3)415-430(2016 Nov)
Hamm MJ,Kirchmaier BC,Herzog W

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

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plxnd1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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