kmt2e gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_005164910.3	XP_005164967.1	histone-lysine N-methyltransferase 2E isoform X4
XM_687873.7	XP_692965.5	histone-lysine N-methyltransferase 2E isoform X2
XM_009300440.2	XP_009298715.1	histone-lysine N-methyltransferase 2E isoform X1
XM_005164909.4	XP_005164966.1	histone-lysine N-methyltransferase 2E isoform X3
XM_009300439.2	XP_009298714.1	histone-lysine N-methyltransferase 2E isoform X1
XM_009300441.2	XP_009298716.1	histone-lysine N-methyltransferase 2E isoform X1
XM_005164909.3	XP_005164966.1	histone-lysine N-methyltransferase 2E isoform X3
XM_009300439.3	XP_009298714.1	histone-lysine N-methyltransferase 2E isoform X1
XM_021475266.1	XP_021330941.1	histone-lysine N-methyltransferase 2E isoform X1
XM_687873.8	XP_692965.5	histone-lysine N-methyltransferase 2E isoform X2
XM_005164910.4	XP_005164967.1	histone-lysine N-methyltransferase 2E isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa09483	XM_687873.8 Latest version!	Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa09484	XM_005164909.4 Latest version!	Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa09485	XM_005164910.4 Latest version!	Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa39844	XM_021475266.1 Latest version!	Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa39844	XM_009300440.2 Latest version!	Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa39844	XM_009300439.3 Latest version!	Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa39844	XM_009300441.2 Latest version!	Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
ODa09484	XM_005164909.3	Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa39844	XM_009300439.2	Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa09483	XM_687873.7	Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa09485	XM_005164910.3	Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

kmt2e ( XM_687873.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_692965.5 Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa09483
Clone ID Related Accession (Same CDS sequence)	XM_687873.7 , XM_687873.8
Accession Version	XM_687873.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	histone-lysine N-methyltransferase 2E isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_687873.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACGGCCAACA CCTCGTATCT GGACATGGCT 
GCAGGCTCAG AACCAGAATC TGTGGAGGCG AGCTCTGTGG TAGTTGAGAA GTCCACCTAC 
CCTCACCAGA TTTACAGCAG CAGCTCCCAC CACTCCCACG GATACATCGG TCTGCCTTAC 
GCCGACCATA ATTATGGGGC GCGACCCCCA CCCACCCCGC CGGCGTCCCC ACCTCCCTCG 
GTGCTGATCC GGCCGGGCGA GAGCTTGTTT GTGTCCGGAA GCCGGCCTGG TGAGAACTTG 
TTTGTGCCGG GCGGGCAGGA CGAGGCGTCG CGTGGCACCA CGCTCAGCAC CTCGGAGGAC 
GGCAGCTACG GTGCTGACAT CACACGCTGC ATCTGTGGCT TCACCCACGA CGATGGCTAC 
ATGATCTGCT GCGACAAGTG CAGTGTGTGG CAGCACATAG ACTGCATGGG GATCGACAGG 
CAGCACATTC CCGAAACCTA CCTGTGTGAG CGCTGTCAGC CACGCACCCT GGACAGAGAG 
CACGCCATCC TCCTGCAAAC CAGGAAGAGA GAGAACATGT CTGATGGGGA CACCAGTGCT 
ACAGAAAGCG GAGATGAGGT TCCACTAGAG CTGTACACAG CGTTTCAGCA CACCCCTACC 
AGCATCACAC TCACAACAGC ACGTCTGGGT AACAAACAGG CTGATAAGAA ACGAAAGAAG 
AGCGGAGAGA AGGAGCCGCC GGCTACAGCC AGGGCCAAGA AGTCATTCCG TGAAGGCTCT 
AGAAAATCAT CTAGAGTAAA GGGCTCCGCT CCCGAGTGTG AGCCGATTGA TCCTCCCTCT 
CTATGGGAGA ATAAGATGAA GTCGTGGATG GAGCGCTACG AGGAGGCCAG CAGTAACCAG 
TACAGTGAAG AAGTGCGGCT ACTGCTGAGA GTCAAAGAAG CCCGAGATGG AAAAACCCTG 
GCCTACAATA CACACACCGC AGCCTTCAAG CCACCTGTAG AGAGTCAAGT ACAAAAAAAC 
AAGCGAATTC TGAAGGCTGT GCGGGATTTG GCTGCTGATT CATTAATAAT CGAGTACAGG 
GGGAAGGTCA TGCTAAGACA GCAGTTTGAG GCTAATGGCT ACTTCTTTAA GAGGCCATAC 
CCTTTTGTAT TGTTCTACTC TAAGTTTGAC GGTTTGGAAA TGTGCGTAGA CGCACGGAGC 
TTTGGGAATG AGGCCCGATT CATTCGGCGC TCCTGCACCC CTAACGCTGA AGTGCGGCAC 
GTGATTGAAG AGGGCATGCT GCATTTATAC ATTTACTCTC TGAGGTCCAT CTCCAAAGGG 
AGCGAGATCA CCATTGGCTT TGATTACGAC TACGGCAGCT GTAAATATAA GGTGGACTGT 
GCTTGTGTAA AAGGCAATCC GGAGTGTCCT GTGCTCAAGC ATAACCTGGA GCCCACAGAG 
AATCTAGGCT CTAGCACACG ACGGCGAGGC CGTAAGGACA AAGAACCATC GCGGGACGAG 
AGCGGACAAA ATCAGAACCT CACTATGGAC TGTGACGGGT CCAAGGGGAA AACGCTTAAT 
GACGCCAAAC AGAGAAAACT CTCTCCTCTC AGGCTCTCAA TATCTAACAA TCAGGATCCT 
GAGTTAATAG AGGATCTAGA AGAGAAAACC TCCGTTAGCA ATGAAGTAGA GATGGAATCA 
GAGGAGCAGA TTGCAGAAAG GAGGAGGAAG ATGACACGGG AAGAGAGGAA GATGGAGGCC 
ATTCTGCAGG CGTTTGCTCG TATGGAGAAG AGGGAAAAGC GGAGGGAGCA AGCGTTGGAG 
AGGATTGGCA CCAAAGGGGA GGTTGGTGGA CGCAGCGATA TAAAGGAGGA GGCACCTGCA 
ACCCCTGAGG CTGAATCTCC TGCCGTGATA CAGCCGCTGC TAGAGGTAGT AAAGGAGGAG 
CCTGGTCTGA AACCGGCGAA GGTGAGCCGT AACAAACAGA GAAAAAGCTT CTCCCGGAAC 
CGGACGCACA TAGGACAGCA GAGGCGGCGA GCACGAACTG TCAGCACCTG CTCCGACTTG 
CCCCCCAGTT CCCCTGGTGA CACTTTAGAG CCCTTAATGG CAGAGACTCA AGATATAGAG 
ATGCCTGTGG CCCCTGAAGC CGATGCTCCG CCAACGCAAC CCCCTGAAAC AAGCCCACCC 
CACAGTGGCT CACCAGCCCC AACATGCCGC AGTGGACAGA AGTACCCCAA AACTAAAAAG 
CACTTAGTGA GTGAGTGGAT GGGTGTTGAC AAACAGGAGC GGGTTGCGTC ACGCACTCCG 
GAGCCTCCCC CTGAAAGGCC GTTGCGGATC AGTAGTGATC CTGAAGTCTT AGCCACACAA 
CTGAACTCTT TACCGGGCAT GTCCTGCAGC TCACACGTCT ACAGCACGCC AAAACACTAC 
GTCCGCTTTT CTTCTCCTTT CCTGGCCAAC CGTAGTCCCA GTACTCCTGG AGTGCCCACG 
GGACGACGGC GCTCGAGAGA GATGCCTGAA ACGCCAACTT CCACTGGCTC CTGCAAGAAG 
CGCTGGCTAA AGCAGGCTTT AGAAGAAGAA GGTTCGACCA GCCCAGGTGG AGGACGACCT 
TTTCTGCTCA TACCCAGTGA GAGTCCTCTC AGTCCCTCTA TAAACGGCGA GTCCTGCAGC 
CCTCTACCCC TCAATGGAAG TTGCTCATTA CCAGAGTTGC CTACACCGTT GAAGAAGAGG 
CGCTTGTGTT CACTCGATCC CTGCATGTCG GAGACTTCCA CCCCATATGG GTCTCCATGT 
GCCACGCCAA CCCGAACAGA GTCAACAGAA GCACCGGGCA CACCTTCGCT GCTAGCAACG 
CCTCCTCGAC CTCGACCAGA GGAGCCCAGT ACCGAGCCCT CGACACCCTT GCAGATGCCC 
AACCACCCTC TGTTACAGGA GAGTGAATCG TCAATGGACA GTTCTCCAGA TGGCAGCCGG 
AGGCCCAGTA CACAAGATGT TGAGCGACCT CCTTCGCTGC TGGCATCTCC CAGCGTGAGA 
AATGTGAGCT CTGATACGGC TCCACAGGAG TCTAGTAAAT CTATGGGGTC TCTGAGTCCT 
CAGCCCTCCC ACACGGAGCC CCAGGATGCC GCAGTGGATG AAGGCATGGA GGTTGATGCT 
GCTGCTGCTG TTACTTCTGC TGCTGGTGGT GGTGGTGGTT CAGAAGCATC AACATCAGCT 
CCTGAAACGC CAGTCTCCTC TTACCCTCCA TGGATGAAGA GTCCAGAGCG GGGCGGCATC 
TCTTTTTCCC CAGTGAATTC AAATCTGAGA GACCTCACTC CTTCACACAC CCTGGAGATG 
GGGGCGTACA GGCCGGATTC AACCTCCAGT GGCCCTTTTA GTGAAGCCGC ACCCTTCTTT 
TCTTGCAATG AGGAGAGCAG TGGTGTGACC TTTACCCGCT CGCTAAGTGG AGATGGCACA 
GGGGAGGGAA GCACTGCAAA AAACCCACAG AAGAAAAAGG TCTCTCTGTT GGAGTACAGA 
AAGCGTCAGC GTGAGGCACG GCGGAGCGGC TCCAAAGGCG AGTGTAGCTC CCCTGTTTCC 
ACAGCACCAC CAGTAGACGT GTTCCCTGTG GCGGTGGAAA TGGCTGTTGA TCCTCCTGCT 
CCTGCAGCAA CACCGACCCC CAGGACCCCG CAGCCTAGTG AGGATCCAGA CGCCCCTCAG 
GGGGAGAGAG ATGGAGAAGG CCAGTGGACA TCTTCCACAT CAGTAGAGCA GGCCAGGGAG 
CGGGGCTACC ACAGGGCCTT ATTGCTGAGT GATCATCGCA AAGATACAGA TGGTGGAGAG 
TCAGAAGGTG GTGATTCCCA AGTGAAGGAC TGTTCTTCTC CCAAGAGCTG CAAGAGCCCC 
TCGACACATG CACCTTGTTC TCCAGCAGCC CAGGCTGTGT CTCGGCCATC AAAGGATGAA 
GATGAAGCAC AGACTCGTGG TCCTGCTCAG CCTCAGGCAG TGTCCGTCCA GCCGTCCAGC 
GCTAAGACTC CCAGCTCAAA ACCAGCTGCT CTGACGCCCA GCAAGCTCCA CTGTGGGCCC 
TCAGGCGCCT CTCAGAGTCA CTACGCGGGA CCTTCGCTCA TGCACTCTCC CAAAGCGCAA 
CCCCAAGGAT CACCTTTCCG TGGCCAGCGA GCCTTTCTGA CAGCTCAGCC TCAGAACCAG 
CCTCAACCAC AAGCTACATC TGGCCCTGCA ACTTTCCCTC AGTATAACCC TCAAAATGCA 
CCTCCACCTC CGCCCCCTCC TCCCCCAGCT CCACCTACAT CTGCTCCTTA CTTCCCCACC 
CAGCCTACTG CTGCTGCTGC TCCCTTTCCT GCATTTAAGC CCACCGTAGC CTCGCCCTTT 
CCTCCTGGTT CCCAGCCTCT ACTTCAGACC CATCATGCAC TGCATTACCA AAGCAGTGCT 
GCTCCTCCTC CTCCACCACC TCCTCCCCCT CCCCACCCGC AGCCTGGCCC CACCTTACTG 
CATGTCAATC TGCAACCTCC TTCACTGCAG CAGCATCAGC TCCTGCTGAG CTCCGCCCCA 
CCTCCGCCTC CTCCTCCACC TCCTCAGACA CAGAGTTCCC AGCAGCCCCC GCCTAACTCC 
GGCACGCTCC TGTCAATCAA ACAAGGACCA CACCCACCCC TTCCACCCCC TCCTCCAGCT 
CCCTCCACTA ACGCACCACC GCACCCGTTC CAAAACATCA GTGGCTTTCA AACCACTCTA 
CTCCACCAGT CTGCACCAGC CAACCCCTCA GTAACCGCAT CTACTTATCA ACAAACTGTA 
TTACCCCCTC CTCCTCCACC TCCCCCCCAA CAAACTCCTC CCACACAGAC CCCGCCCAAT 
CCGAGCATCC CTCAGATCGC AGGTGGCAAT CGAGGACCCG CTCCTTCCTC CGCCTCTTTC 
CACACCACCG GATACTTGGG CACAGGATGG CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_692965.5
CDS	1143..6038
Translation	MSIVIPVGVDTANTSYLDMAAGSEPESVEASSVVVEKSTYPHQIYSSSSHHSHGYIGLPYADHNYGARPPPTPPASPPPSVLIRPGESLFVSGSRPGENLFVPGGQDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQHIPETYLCERCQPRTLDREHAILLQTRKRENMSDGDTSATESGDEVPLELYTAFQHTPTSITLTTARLGNKQADKKRKKSGEKEPPATARAKKSFREGSRKSSRVKGSAPECEPIDPPSLWENKMKSWMERYEEASSNQYSEEVRLLLRVKEARDGKTLAYNTHTAAFKPPVESQVQKNKRILKAVRDLAADSLIIEYRGKVMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARSFGNEARFIRRSCTPNAEVRHVIEEGMLHLYIYSLRSISKGSEITIGFDYDYGSCKYKVDCACVKGNPECPVLKHNLEPTENLGSSTRRRGRKDKEPSRDESGQNQNLTMDCDGSKGKTLNDAKQRKLSPLRLSISNNQDPELIEDLEEKTSVSNEVEMESEEQIAERRRKMTREERKMEAILQAFARMEKREKRREQALERIGTKGEVGGRSDIKEEAPATPEAESPAVIQPLLEVVKEEPGLKPAKVSRNKQRKSFSRNRTHIGQQRRRARTVSTCSDLPPSSPGDTLEPLMAETQDIEMPVAPEADAPPTQPPETSPPHSGSPAPTCRSGQKYPKTKKHLVSEWMGVDKQERVASRTPEPPPERPLRISSDPEVLATQLNSLPGMSCSSHVYSTPKHYVRFSSPFLANRSPSTPGVPTGRRRSREMPETPTSTGSCKKRWLKQALEEEGSTSPGGGRPFLLIPSESPLSPSINGESCSPLPLNGSCSLPELPTPLKKRRLCSLDPCMSETSTPYGSPCATPTRTESTEAPGTPSLLATPPRPRPEEPSTEPSTPLQMPNHPLLQESESSMDSSPDGSRRPSTQDVERPPSLLASPSVRNVSSDTAPQESSKSMGSLSPQPSHTEPQDAAVDEGMEVDAAAAVTSAAGGGGGSEASTSAPETPVSSYPPWMKSPERGGISFSPVNSNLRDLTPSHTLEMGAYRPDSTSSGPFSEAAPFFSCNEESSGVTFTRSLSGDGTGEGSTAKNPQKKKVSLLEYRKRQREARRSGSKGECSSPVSTAPPVDVFPVAVEMAVDPPAPAATPTPRTPQPSEDPDAPQGERDGEGQWTSSTSVEQARERGYHRALLLSDHRKDTDGGESEGGDSQVKDCSSPKSCKSPSTHAPCSPAAQAVSRPSKDEDEAQTRGPAQPQAVSVQPSSAKTPSSKPAALTPSKLHCGPSGASQSHYAGPSLMHSPKAQPQGSPFRGQRAFLTAQPQNQPQPQATSGPATFPQYNPQNAPPPPPPPPPAPPTSAPYFPTQPTAAAAPFPAFKPTVASPFPPGSQPLLQTHHALHYQSSAAPPPPPPPPPPHPQPGPTLLHVNLQPPSLQQHQLLLSSAPPPPPPPPPQTQSSQQPPPNSGTLLSIKQGPHPPLPPPPPAPSTNAPPHPFQNISGFQTTLLHQSAPANPSVTASTYQQTVLPPPPPPPPQQTPPTQTPPNPSIPQIAGGNRGPAPSSASFHTTGYLGTGWH

Target ORF information:

RefSeq Version	XM_687873.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_687873.7

ORF Insert Sequence:

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACGGCCAACA CCTCGTATCT GGACATGGCT 
GCAGGCTCAG AACCAGAATC TGTGGAGGCG AGCTCTGTGG TAGTTGAGAA GTCCACCTAC 
CCTCACCAGA TTTACAGCAG CAGCTCCCAC CACTCCCACG GATACATCGG TCTGCCTTAC 
GCCGACCATA ATTATGGGGC GCGACCCCCA CCCACCCCGC CGGCGTCCCC ACCTCCCTCG 
GTGCTGATCC GGCCGGGCGA GAGCTTGTTT GTGTCCGGAA GCCGGCCTGG TGAGAACTTG 
TTTGTGCCGG GCGGGCAGGA CGAGGCGTCG CGTGGCACCA CGCTCAGCAC CTCGGAGGAC 
GGCAGCTACG GTGCTGACAT CACACGCTGC ATCTGTGGCT TCACCCACGA CGATGGCTAC 
ATGATCTGCT GCGACAAGTG CAGTGTGTGG CAGCACATAG ACTGCATGGG GATCGACAGG 
CAGCACATTC CCGAAACCTA CCTGTGTGAG CGCTGTCAGC CACGCACCCT GGACAGAGAG 
CACGCCATCC TCCTGCAAAC CAGGAAGAGA GAGAACATGT CTGATGGGGA CACCAGTGCT 
ACAGAAAGCG GAGATGAGGT TCCACTAGAG CTGTACACAG CGTTTCAGCA CACCCCTACC 
AGCATCACAC TCACAACAGC ACGTCTGGGT AACAAACAGG CTGATAAGAA ACGAAAGAAG 
AGCGGAGAGA AGGAGCCGCC GGCTACAGCC AGGGCCAAGA AGTCATTCCG TGAAGGCTCT 
AGAAAATCAT CTAGAGTAAA GGGCTCCGCT CCCGAGTGTG AGCCGATTGA TCCTCCCTCT 
CTATGGGAGA ATAAGATGAA GTCGTGGATG GAGCGCTACG AGGAGGCCAG CAGTAACCAG 
TACAGTGAAG AAGTGCGGCT ACTGCTGAGA GTCAAAGAAG CCCGAGATGG AAAAACCCTG 
GCCTACAATA CACACACCGC AGCCTTCAAG CCACCTGTAG AGAGTCAAGT ACAAAAAAAC 
AAGCGAATTC TGAAGGCTGT GCGGGATTTG GCTGCTGATT CATTAATAAT CGAGTACAGG 
GGGAAGGTCA TGCTAAGACA GCAGTTTGAG GCTAATGGCT ACTTCTTTAA GAGGCCATAC 
CCTTTTGTAT TGTTCTACTC TAAGTTTGAC GGTTTGGAAA TGTGCGTAGA CGCACGGAGC 
TTTGGGAATG AGGCCCGATT CATTCGGCGC TCCTGCACCC CTAACGCTGA AGTGCGGCAC 
GTGATTGAAG AGGGCATGCT GCATTTATAC ATTTACTCTC TGAGGTCCAT CTCCAAAGGG 
AGCGAGATCA CCATTGGCTT TGATTACGAC TACGGCAGCT GTAAATATAA GGTGGACTGT 
GCTTGTGTAA AAGGCAATCC GGAGTGTCCT GTGCTCAAGC ATAACCTGGA GCCCACAGAG 
AATCTAGGCT CTAGCACACG ACGGCGAGGC CGTAAGGACA AAGAACCATC GCGGGACGAG 
AGCGGACAAA ATCAGAACCT CACTATGGAC TGTGACGGGT CCAAGGGGAA AACGCTTAAT 
GACGCCAAAC AGAGAAAACT CTCTCCTCTC AGGCTCTCAA TATCTAACAA TCAGGATCCT 
GAGTTAATAG AGGATCTAGA AGAGAAAACC TCCGTTAGCA ATGAAGTAGA GATGGAATCA 
GAGGAGCAGA TTGCAGAAAG GAGGAGGAAG ATGACACGGG AAGAGAGGAA GATGGAGGCC 
ATTCTGCAGG CGTTTGCTCG TATGGAGAAG AGGGAAAAGC GGAGGGAGCA AGCGTTGGAG 
AGGATTGGCA CCAAAGGGGA GGTTGGTGGA CGCAGCGATA TAAAGGAGGA GGCACCTGCA 
ACCCCTGAGG CTGAATCTCC TGCCGTGATA CAGCCGCTGC TAGAGGTAGT AAAGGAGGAG 
CCTGGTCTGA AACCGGCGAA GGTGAGCCGT AACAAACAGA GAAAAAGCTT CTCCCGGAAC 
CGGACGCACA TAGGACAGCA GAGGCGGCGA GCACGAACTG TCAGCACCTG CTCCGACTTG 
CCCCCCAGTT CCCCTGGTGA CACTTTAGAG CCCTTAATGG CAGAGACTCA AGATATAGAG 
ATGCCTGTGG CCCCTGAAGC CGATGCTCCG CCAACGCAAC CCCCTGAAAC AAGCCCACCC 
CACAGTGGCT CACCAGCCCC AACATGCCGC AGTGGACAGA AGTACCCCAA AACTAAAAAG 
CACTTAGTGA GTGAGTGGAT GGGTGTTGAC AAACAGGAGC GGGTTGCGTC ACGCACTCCG 
GAGCCTCCCC CTGAAAGGCC GTTGCGGATC AGTAGTGATC CTGAAGTCTT AGCCACACAA 
CTGAACTCTT TACCGGGCAT GTCCTGCAGC TCACACGTCT ACAGCACGCC AAAACACTAC 
GTCCGCTTTT CTTCTCCTTT CCTGGCCAAC CGTAGTCCCA GTACTCCTGG AGTGCCCACG 
GGACGACGGC GCTCGAGAGA GATGCCTGAA ACGCCAACTT CCACTGGCTC CTGCAAGAAG 
CGCTGGCTAA AGCAGGCTTT AGAAGAAGAA GGTTCGACCA GCCCAGGTGG AGGACGACCT 
TTTCTGCTCA TACCCAGTGA GAGTCCTCTC AGTCCCTCTA TAAACGGCGA GTCCTGCAGC 
CCTCTACCCC TCAATGGAAG TTGCTCATTA CCAGAGTTGC CTACACCGTT GAAGAAGAGG 
CGCTTGTGTT CACTCGATCC CTGCATGTCG GAGACTTCCA CCCCATATGG GTCTCCATGT 
GCCACGCCAA CCCGAACAGA GTCAACAGAA GCACCGGGCA CACCTTCGCT GCTAGCAACG 
CCTCCTCGAC CTCGACCAGA GGAGCCCAGT ACCGAGCCCT CGACACCCTT GCAGATGCCC 
AACCACCCTC TGTTACAGGA GAGTGAATCG TCAATGGACA GTTCTCCAGA TGGCAGCCGG 
AGGCCCAGTA CACAAGATGT TGAGCGACCT CCTTCGCTGC TGGCATCTCC CAGCGTGAGA 
AATGTGAGCT CTGATACGGC TCCACAGGAG TCTAGTAAAT CTATGGGGTC TCTGAGTCCT 
CAGCCCTCCC ACACGGAGCC CCAGGATGCC GCAGTGGATG AAGGCATGGA GGTTGATGCT 
GCTGCTGCTG TTACTTCTGC TGCTGGTGGT GGTGGTGGTT CAGAAGCATC AACATCAGCT 
CCTGAAACGC CAGTCTCCTC TTACCCTCCA TGGATGAAGA GTCCAGAGCG GGGCGGCATC 
TCTTTTTCCC CAGTGAATTC AAATCTGAGA GACCTCACTC CTTCACACAC CCTGGAGATG 
GGGGCGTACA GGCCGGATTC AACCTCCAGT GGCCCTTTTA GTGAAGCCGC ACCCTTCTTT 
TCTTGCAATG AGGAGAGCAG TGGTGTGACC TTTACCCGCT CGCTAAGTGG AGATGGCACA 
GGGGAGGGAA GCACTGCAAA AAACCCACAG AAGAAAAAGG TCTCTCTGTT GGAGTACAGA 
AAGCGTCAGC GTGAGGCACG GCGGAGCGGC TCCAAAGGCG AGTGTAGCTC CCCTGTTTCC 
ACAGCACCAC CAGTAGACGT GTTCCCTGTG GCGGTGGAAA TGGCTGTTGA TCCTCCTGCT 
CCTGCAGCAA CACCGACCCC CAGGACCCCG CAGCCTAGTG AGGATCCAGA CGCCCCTCAG 
GGGGAGAGAG ATGGAGAAGG CCAGTGGACA TCTTCCACAT CAGTAGAGCA GGCCAGGGAG 
CGGGGCTACC ACAGGGCCTT ATTGCTGAGT GATCATCGCA AAGATACAGA TGGTGGAGAG 
TCAGAAGGTG GTGATTCCCA AGTGAAGGAC TGTTCTTCTC CCAAGAGCTG CAAGAGCCCC 
TCGACACATG CACCTTGTTC TCCAGCAGCC CAGGCTGTGT CTCGGCCATC AAAGGATGAA 
GATGAAGCAC AGACTCGTGG TCCTGCTCAG CCTCAGGCAG TGTCCGTCCA GCCGTCCAGC 
GCTAAGACTC CCAGCTCAAA ACCAGCTGCT CTGACGCCCA GCAAGCTCCA CTGTGGGCCC 
TCAGGCGCCT CTCAGAGTCA CTACGCGGGA CCTTCGCTCA TGCACTCTCC CAAAGCGCAA 
CCCCAAGGAT CACCTTTCCG TGGCCAGCGA GCCTTTCTGA CAGCTCAGCC TCAGAACCAG 
CCTCAACCAC AAGCTACATC TGGCCCTGCA ACTTTCCCTC AGTATAACCC TCAAAATGCA 
CCTCCACCTC CGCCCCCTCC TCCCCCAGCT CCACCTACAT CTGCTCCTTA CTTCCCCACC 
CAGCCTACTG CTGCTGCTGC TCCCTTTCCT GCATTTAAGC CCACCGTAGC CTCGCCCTTT 
CCTCCTGGTT CCCAGCCTCT ACTTCAGACC CATCATGCAC TGCATTACCA AAGCAGTGCT 
GCTCCTCCTC CTCCACCACC TCCTCCCCCT CCCCACCCGC AGCCTGGCCC CACCTTACTG 
CATGTCAATC TGCAACCTCC TTCACTGCAG CAGCATCAGC TCCTGCTGAG CTCCGCCCCA 
CCTCCGCCTC CTCCTCCACC TCCTCAGACA CAGAGTTCCC AGCAGCCCCC GCCTAACTCC 
GGCACGCTCC TGTCAATCAA ACAAGGACCA CACCCACCCC TTCCACCCCC TCCTCCAGCT 
CCCTCCACTA ACGCACCACC GCACCCGTTC CAAAACATCA GTGGCTTTCA AACCACTCTA 
CTCCACCAGT CTGCACCAGC CAACCCCTCA GTAACCGCAT CTACTTATCA ACAAACTGTA 
TTACCCCCTC CTCCTCCACC TCCCCCCCAA CAAACTCCTC CCACACAGAC CCCGCCCAAT 
CCGAGCATCC CTCAGATCGC AGGTGGCAAT CGAGGACCCG CTCCTTCCTC CGCCTCTTTC 
CACACCACCG GATACTTGGG CACAGGATGG CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_687873.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_692965.5 Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa09483
Clone ID Related Accession (Same CDS sequence)	XM_687873.7 , XM_687873.8
Accession Version	XM_687873.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	histone-lysine N-methyltransferase 2E isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_687873.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACGGCCAACA CCTCGTATCT GGACATGGCT 
GCAGGCTCAG AACCAGAATC TGTGGAGGCG AGCTCTGTGG TAGTTGAGAA GTCCACCTAC 
CCTCACCAGA TTTACAGCAG CAGCTCCCAC CACTCCCACG GATACATCGG TCTGCCTTAC 
GCCGACCATA ATTATGGGGC GCGACCCCCA CCCACCCCGC CGGCGTCCCC ACCTCCCTCG 
GTGCTGATCC GGCCGGGCGA GAGCTTGTTT GTGTCCGGAA GCCGGCCTGG TGAGAACTTG 
TTTGTGCCGG GCGGGCAGGA CGAGGCGTCG CGTGGCACCA CGCTCAGCAC CTCGGAGGAC 
GGCAGCTACG GTGCTGACAT CACACGCTGC ATCTGTGGCT TCACCCACGA CGATGGCTAC 
ATGATCTGCT GCGACAAGTG CAGTGTGTGG CAGCACATAG ACTGCATGGG GATCGACAGG 
CAGCACATTC CCGAAACCTA CCTGTGTGAG CGCTGTCAGC CACGCACCCT GGACAGAGAG 
CACGCCATCC TCCTGCAAAC CAGGAAGAGA GAGAACATGT CTGATGGGGA CACCAGTGCT 
ACAGAAAGCG GAGATGAGGT TCCACTAGAG CTGTACACAG CGTTTCAGCA CACCCCTACC 
AGCATCACAC TCACAACAGC ACGTCTGGGT AACAAACAGG CTGATAAGAA ACGAAAGAAG 
AGCGGAGAGA AGGAGCCGCC GGCTACAGCC AGGGCCAAGA AGTCATTCCG TGAAGGCTCT 
AGAAAATCAT CTAGAGTAAA GGGCTCCGCT CCCGAGTGTG AGCCGATTGA TCCTCCCTCT 
CTATGGGAGA ATAAGATGAA GTCGTGGATG GAGCGCTACG AGGAGGCCAG CAGTAACCAG 
TACAGTGAAG AAGTGCGGCT ACTGCTGAGA GTCAAAGAAG CCCGAGATGG AAAAACCCTG 
GCCTACAATA CACACACCGC AGCCTTCAAG CCACCTGTAG AGAGTCAAGT ACAAAAAAAC 
AAGCGAATTC TGAAGGCTGT GCGGGATTTG GCTGCTGATT CATTAATAAT CGAGTACAGG 
GGGAAGGTCA TGCTAAGACA GCAGTTTGAG GCTAATGGCT ACTTCTTTAA GAGGCCATAC 
CCTTTTGTAT TGTTCTACTC TAAGTTTGAC GGTTTGGAAA TGTGCGTAGA CGCACGGAGC 
TTTGGGAATG AGGCCCGATT CATTCGGCGC TCCTGCACCC CTAACGCTGA AGTGCGGCAC 
GTGATTGAAG AGGGCATGCT GCATTTATAC ATTTACTCTC TGAGGTCCAT CTCCAAAGGG 
AGCGAGATCA CCATTGGCTT TGATTACGAC TACGGCAGCT GTAAATATAA GGTGGACTGT 
GCTTGTGTAA AAGGCAATCC GGAGTGTCCT GTGCTCAAGC ATAACCTGGA GCCCACAGAG 
AATCTAGGCT CTAGCACACG ACGGCGAGGC CGTAAGGACA AAGAACCATC GCGGGACGAG 
AGCGGACAAA ATCAGAACCT CACTATGGAC TGTGACGGGT CCAAGGGGAA AACGCTTAAT 
GACGCCAAAC AGAGAAAACT CTCTCCTCTC AGGCTCTCAA TATCTAACAA TCAGGATCCT 
GAGTTAATAG AGGATCTAGA AGAGAAAACC TCCGTTAGCA ATGAAGTAGA GATGGAATCA 
GAGGAGCAGA TTGCAGAAAG GAGGAGGAAG ATGACACGGG AAGAGAGGAA GATGGAGGCC 
ATTCTGCAGG CGTTTGCTCG TATGGAGAAG AGGGAAAAGC GGAGGGAGCA AGCGTTGGAG 
AGGATTGGCA CCAAAGGGGA GGTTGGTGGA CGCAGCGATA TAAAGGAGGA GGCACCTGCA 
ACCCCTGAGG CTGAATCTCC TGCCGTGATA CAGCCGCTGC TAGAGGTAGT AAAGGAGGAG 
CCTGGTCTGA AACCGGCGAA GGTGAGCCGT AACAAACAGA GAAAAAGCTT CTCCCGGAAC 
CGGACGCACA TAGGACAGCA GAGGCGGCGA GCACGAACTG TCAGCACCTG CTCCGACTTG 
CCCCCCAGTT CCCCTGGTGA CACTTTAGAG CCCTTAATGG CAGAGACTCA AGATATAGAG 
ATGCCTGTGG CCCCTGAAGC CGATGCTCCG CCAACGCAAC CCCCTGAAAC AAGCCCACCC 
CACAGTGGCT CACCAGCCCC AACATGCCGC AGTGGACAGA AGTACCCCAA AACTAAAAAG 
CACTTAGTGA GTGAGTGGAT GGGTGTTGAC AAACAGGAGC GGGTTGCGTC ACGCACTCCG 
GAGCCTCCCC CTGAAAGGCC GTTGCGGATC AGTAGTGATC CTGAAGTCTT AGCCACACAA 
CTGAACTCTT TACCGGGCAT GTCCTGCAGC TCACACGTCT ACAGCACGCC AAAACACTAC 
GTCCGCTTTT CTTCTCCTTT CCTGGCCAAC CGTAGTCCCA GTACTCCTGG AGTGCCCACG 
GGACGACGGC GCTCGAGAGA GATGCCTGAA ACGCCAACTT CCACTGGCTC CTGCAAGAAG 
CGCTGGCTAA AGCAGGCTTT AGAAGAAGAA GGTTCGACCA GCCCAGGTGG AGGACGACCT 
TTTCTGCTCA TACCCAGTGA GAGTCCTCTC AGTCCCTCTA TAAACGGCGA GTCCTGCAGC 
CCTCTACCCC TCAATGGAAG TTGCTCATTA CCAGAGTTGC CTACACCGTT GAAGAAGAGG 
CGCTTGTGTT CACTCGATCC CTGCATGTCG GAGACTTCCA CCCCATATGG GTCTCCATGT 
GCCACGCCAA CCCGAACAGA GTCAACAGAA GCACCGGGCA CACCTTCGCT GCTAGCAACG 
CCTCCTCGAC CTCGACCAGA GGAGCCCAGT ACCGAGCCCT CGACACCCTT GCAGATGCCC 
AACCACCCTC TGTTACAGGA GAGTGAATCG TCAATGGACA GTTCTCCAGA TGGCAGCCGG 
AGGCCCAGTA CACAAGATGT TGAGCGACCT CCTTCGCTGC TGGCATCTCC CAGCGTGAGA 
AATGTGAGCT CTGATACGGC TCCACAGGAG TCTAGTAAAT CTATGGGGTC TCTGAGTCCT 
CAGCCCTCCC ACACGGAGCC CCAGGATGCC GCAGTGGATG AAGGCATGGA GGTTGATGCT 
GCTGCTGCTG TTACTTCTGC TGCTGGTGGT GGTGGTGGTT CAGAAGCATC AACATCAGCT 
CCTGAAACGC CAGTCTCCTC TTACCCTCCA TGGATGAAGA GTCCAGAGCG GGGCGGCATC 
TCTTTTTCCC CAGTGAATTC AAATCTGAGA GACCTCACTC CTTCACACAC CCTGGAGATG 
GGGGCGTACA GGCCGGATTC AACCTCCAGT GGCCCTTTTA GTGAAGCCGC ACCCTTCTTT 
TCTTGCAATG AGGAGAGCAG TGGTGTGACC TTTACCCGCT CGCTAAGTGG AGATGGCACA 
GGGGAGGGAA GCACTGCAAA AAACCCACAG AAGAAAAAGG TCTCTCTGTT GGAGTACAGA 
AAGCGTCAGC GTGAGGCACG GCGGAGCGGC TCCAAAGGCG AGTGTAGCTC CCCTGTTTCC 
ACAGCACCAC CAGTAGACGT GTTCCCTGTG GCGGTGGAAA TGGCTGTTGA TCCTCCTGCT 
CCTGCAGCAA CACCGACCCC CAGGACCCCG CAGCCTAGTG AGGATCCAGA CGCCCCTCAG 
GGGGAGAGAG ATGGAGAAGG CCAGTGGACA TCTTCCACAT CAGTAGAGCA GGCCAGGGAG 
CGGGGCTACC ACAGGGCCTT ATTGCTGAGT GATCATCGCA AAGATACAGA TGGTGGAGAG 
TCAGAAGGTG GTGATTCCCA AGTGAAGGAC TGTTCTTCTC CCAAGAGCTG CAAGAGCCCC 
TCGACACATG CACCTTGTTC TCCAGCAGCC CAGGCTGTGT CTCGGCCATC AAAGGATGAA 
GATGAAGCAC AGACTCGTGG TCCTGCTCAG CCTCAGGCAG TGTCCGTCCA GCCGTCCAGC 
GCTAAGACTC CCAGCTCAAA ACCAGCTGCT CTGACGCCCA GCAAGCTCCA CTGTGGGCCC 
TCAGGCGCCT CTCAGAGTCA CTACGCGGGA CCTTCGCTCA TGCACTCTCC CAAAGCGCAA 
CCCCAAGGAT CACCTTTCCG TGGCCAGCGA GCCTTTCTGA CAGCTCAGCC TCAGAACCAG 
CCTCAACCAC AAGCTACATC TGGCCCTGCA ACTTTCCCTC AGTATAACCC TCAAAATGCA 
CCTCCACCTC CGCCCCCTCC TCCCCCAGCT CCACCTACAT CTGCTCCTTA CTTCCCCACC 
CAGCCTACTG CTGCTGCTGC TCCCTTTCCT GCATTTAAGC CCACCGTAGC CTCGCCCTTT 
CCTCCTGGTT CCCAGCCTCT ACTTCAGACC CATCATGCAC TGCATTACCA AAGCAGTGCT 
GCTCCTCCTC CTCCACCACC TCCTCCCCCT CCCCACCCGC AGCCTGGCCC CACCTTACTG 
CATGTCAATC TGCAACCTCC TTCACTGCAG CAGCATCAGC TCCTGCTGAG CTCCGCCCCA 
CCTCCGCCTC CTCCTCCACC TCCTCAGACA CAGAGTTCCC AGCAGCCCCC GCCTAACTCC 
GGCACGCTCC TGTCAATCAA ACAAGGACCA CACCCACCCC TTCCACCCCC TCCTCCAGCT 
CCCTCCACTA ACGCACCACC GCACCCGTTC CAAAACATCA GTGGCTTTCA AACCACTCTA 
CTCCACCAGT CTGCACCAGC CAACCCCTCA GTAACCGCAT CTACTTATCA ACAAACTGTA 
TTACCCCCTC CTCCTCCACC TCCCCCCCAA CAAACTCCTC CCACACAGAC CCCGCCCAAT 
CCGAGCATCC CTCAGATCGC AGGTGGCAAT CGAGGACCCG CTCCTTCCTC CGCCTCTTTC 
CACACCACCG GATACTTGGG CACAGGATGG CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_692965.5
CDS	1082..5977
Translation	MSIVIPVGVDTANTSYLDMAAGSEPESVEASSVVVEKSTYPHQIYSSSSHHSHGYIGLPYADHNYGARPPPTPPASPPPSVLIRPGESLFVSGSRPGENLFVPGGQDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQHIPETYLCERCQPRTLDREHAILLQTRKRENMSDGDTSATESGDEVPLELYTAFQHTPTSITLTTARLGNKQADKKRKKSGEKEPPATARAKKSFREGSRKSSRVKGSAPECEPIDPPSLWENKMKSWMERYEEASSNQYSEEVRLLLRVKEARDGKTLAYNTHTAAFKPPVESQVQKNKRILKAVRDLAADSLIIEYRGKVMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARSFGNEARFIRRSCTPNAEVRHVIEEGMLHLYIYSLRSISKGSEITIGFDYDYGSCKYKVDCACVKGNPECPVLKHNLEPTENLGSSTRRRGRKDKEPSRDESGQNQNLTMDCDGSKGKTLNDAKQRKLSPLRLSISNNQDPELIEDLEEKTSVSNEVEMESEEQIAERRRKMTREERKMEAILQAFARMEKREKRREQALERIGTKGEVGGRSDIKEEAPATPEAESPAVIQPLLEVVKEEPGLKPAKVSRNKQRKSFSRNRTHIGQQRRRARTVSTCSDLPPSSPGDTLEPLMAETQDIEMPVAPEADAPPTQPPETSPPHSGSPAPTCRSGQKYPKTKKHLVSEWMGVDKQERVASRTPEPPPERPLRISSDPEVLATQLNSLPGMSCSSHVYSTPKHYVRFSSPFLANRSPSTPGVPTGRRRSREMPETPTSTGSCKKRWLKQALEEEGSTSPGGGRPFLLIPSESPLSPSINGESCSPLPLNGSCSLPELPTPLKKRRLCSLDPCMSETSTPYGSPCATPTRTESTEAPGTPSLLATPPRPRPEEPSTEPSTPLQMPNHPLLQESESSMDSSPDGSRRPSTQDVERPPSLLASPSVRNVSSDTAPQESSKSMGSLSPQPSHTEPQDAAVDEGMEVDAAAAVTSAAGGGGGSEASTSAPETPVSSYPPWMKSPERGGISFSPVNSNLRDLTPSHTLEMGAYRPDSTSSGPFSEAAPFFSCNEESSGVTFTRSLSGDGTGEGSTAKNPQKKKVSLLEYRKRQREARRSGSKGECSSPVSTAPPVDVFPVAVEMAVDPPAPAATPTPRTPQPSEDPDAPQGERDGEGQWTSSTSVEQARERGYHRALLLSDHRKDTDGGESEGGDSQVKDCSSPKSCKSPSTHAPCSPAAQAVSRPSKDEDEAQTRGPAQPQAVSVQPSSAKTPSSKPAALTPSKLHCGPSGASQSHYAGPSLMHSPKAQPQGSPFRGQRAFLTAQPQNQPQPQATSGPATFPQYNPQNAPPPPPPPPPAPPTSAPYFPTQPTAAAAPFPAFKPTVASPFPPGSQPLLQTHHALHYQSSAAPPPPPPPPPPHPQPGPTLLHVNLQPPSLQQHQLLLSSAPPPPPPPPPQTQSSQQPPPNSGTLLSIKQGPHPPLPPPPPAPSTNAPPHPFQNISGFQTTLLHQSAPANPSVTASTYQQTVLPPPPPPPPQQTPPTQTPPNPSIPQIAGGNRGPAPSSASFHTTGYLGTGWH

Target ORF information:

RefSeq Version	XM_687873.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_687873.8

ORF Insert Sequence:

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACGGCCAACA CCTCGTATCT GGACATGGCT 
GCAGGCTCAG AACCAGAATC TGTGGAGGCG AGCTCTGTGG TAGTTGAGAA GTCCACCTAC 
CCTCACCAGA TTTACAGCAG CAGCTCCCAC CACTCCCACG GATACATCGG TCTGCCTTAC 
GCCGACCATA ATTATGGGGC GCGACCCCCA CCCACCCCGC CGGCGTCCCC ACCTCCCTCG 
GTGCTGATCC GGCCGGGCGA GAGCTTGTTT GTGTCCGGAA GCCGGCCTGG TGAGAACTTG 
TTTGTGCCGG GCGGGCAGGA CGAGGCGTCG CGTGGCACCA CGCTCAGCAC CTCGGAGGAC 
GGCAGCTACG GTGCTGACAT CACACGCTGC ATCTGTGGCT TCACCCACGA CGATGGCTAC 
ATGATCTGCT GCGACAAGTG CAGTGTGTGG CAGCACATAG ACTGCATGGG GATCGACAGG 
CAGCACATTC CCGAAACCTA CCTGTGTGAG CGCTGTCAGC CACGCACCCT GGACAGAGAG 
CACGCCATCC TCCTGCAAAC CAGGAAGAGA GAGAACATGT CTGATGGGGA CACCAGTGCT 
ACAGAAAGCG GAGATGAGGT TCCACTAGAG CTGTACACAG CGTTTCAGCA CACCCCTACC 
AGCATCACAC TCACAACAGC ACGTCTGGGT AACAAACAGG CTGATAAGAA ACGAAAGAAG 
AGCGGAGAGA AGGAGCCGCC GGCTACAGCC AGGGCCAAGA AGTCATTCCG TGAAGGCTCT 
AGAAAATCAT CTAGAGTAAA GGGCTCCGCT CCCGAGTGTG AGCCGATTGA TCCTCCCTCT 
CTATGGGAGA ATAAGATGAA GTCGTGGATG GAGCGCTACG AGGAGGCCAG CAGTAACCAG 
TACAGTGAAG AAGTGCGGCT ACTGCTGAGA GTCAAAGAAG CCCGAGATGG AAAAACCCTG 
GCCTACAATA CACACACCGC AGCCTTCAAG CCACCTGTAG AGAGTCAAGT ACAAAAAAAC 
AAGCGAATTC TGAAGGCTGT GCGGGATTTG GCTGCTGATT CATTAATAAT CGAGTACAGG 
GGGAAGGTCA TGCTAAGACA GCAGTTTGAG GCTAATGGCT ACTTCTTTAA GAGGCCATAC 
CCTTTTGTAT TGTTCTACTC TAAGTTTGAC GGTTTGGAAA TGTGCGTAGA CGCACGGAGC 
TTTGGGAATG AGGCCCGATT CATTCGGCGC TCCTGCACCC CTAACGCTGA AGTGCGGCAC 
GTGATTGAAG AGGGCATGCT GCATTTATAC ATTTACTCTC TGAGGTCCAT CTCCAAAGGG 
AGCGAGATCA CCATTGGCTT TGATTACGAC TACGGCAGCT GTAAATATAA GGTGGACTGT 
GCTTGTGTAA AAGGCAATCC GGAGTGTCCT GTGCTCAAGC ATAACCTGGA GCCCACAGAG 
AATCTAGGCT CTAGCACACG ACGGCGAGGC CGTAAGGACA AAGAACCATC GCGGGACGAG 
AGCGGACAAA ATCAGAACCT CACTATGGAC TGTGACGGGT CCAAGGGGAA AACGCTTAAT 
GACGCCAAAC AGAGAAAACT CTCTCCTCTC AGGCTCTCAA TATCTAACAA TCAGGATCCT 
GAGTTAATAG AGGATCTAGA AGAGAAAACC TCCGTTAGCA ATGAAGTAGA GATGGAATCA 
GAGGAGCAGA TTGCAGAAAG GAGGAGGAAG ATGACACGGG AAGAGAGGAA GATGGAGGCC 
ATTCTGCAGG CGTTTGCTCG TATGGAGAAG AGGGAAAAGC GGAGGGAGCA AGCGTTGGAG 
AGGATTGGCA CCAAAGGGGA GGTTGGTGGA CGCAGCGATA TAAAGGAGGA GGCACCTGCA 
ACCCCTGAGG CTGAATCTCC TGCCGTGATA CAGCCGCTGC TAGAGGTAGT AAAGGAGGAG 
CCTGGTCTGA AACCGGCGAA GGTGAGCCGT AACAAACAGA GAAAAAGCTT CTCCCGGAAC 
CGGACGCACA TAGGACAGCA GAGGCGGCGA GCACGAACTG TCAGCACCTG CTCCGACTTG 
CCCCCCAGTT CCCCTGGTGA CACTTTAGAG CCCTTAATGG CAGAGACTCA AGATATAGAG 
ATGCCTGTGG CCCCTGAAGC CGATGCTCCG CCAACGCAAC CCCCTGAAAC AAGCCCACCC 
CACAGTGGCT CACCAGCCCC AACATGCCGC AGTGGACAGA AGTACCCCAA AACTAAAAAG 
CACTTAGTGA GTGAGTGGAT GGGTGTTGAC AAACAGGAGC GGGTTGCGTC ACGCACTCCG 
GAGCCTCCCC CTGAAAGGCC GTTGCGGATC AGTAGTGATC CTGAAGTCTT AGCCACACAA 
CTGAACTCTT TACCGGGCAT GTCCTGCAGC TCACACGTCT ACAGCACGCC AAAACACTAC 
GTCCGCTTTT CTTCTCCTTT CCTGGCCAAC CGTAGTCCCA GTACTCCTGG AGTGCCCACG 
GGACGACGGC GCTCGAGAGA GATGCCTGAA ACGCCAACTT CCACTGGCTC CTGCAAGAAG 
CGCTGGCTAA AGCAGGCTTT AGAAGAAGAA GGTTCGACCA GCCCAGGTGG AGGACGACCT 
TTTCTGCTCA TACCCAGTGA GAGTCCTCTC AGTCCCTCTA TAAACGGCGA GTCCTGCAGC 
CCTCTACCCC TCAATGGAAG TTGCTCATTA CCAGAGTTGC CTACACCGTT GAAGAAGAGG 
CGCTTGTGTT CACTCGATCC CTGCATGTCG GAGACTTCCA CCCCATATGG GTCTCCATGT 
GCCACGCCAA CCCGAACAGA GTCAACAGAA GCACCGGGCA CACCTTCGCT GCTAGCAACG 
CCTCCTCGAC CTCGACCAGA GGAGCCCAGT ACCGAGCCCT CGACACCCTT GCAGATGCCC 
AACCACCCTC TGTTACAGGA GAGTGAATCG TCAATGGACA GTTCTCCAGA TGGCAGCCGG 
AGGCCCAGTA CACAAGATGT TGAGCGACCT CCTTCGCTGC TGGCATCTCC CAGCGTGAGA 
AATGTGAGCT CTGATACGGC TCCACAGGAG TCTAGTAAAT CTATGGGGTC TCTGAGTCCT 
CAGCCCTCCC ACACGGAGCC CCAGGATGCC GCAGTGGATG AAGGCATGGA GGTTGATGCT 
GCTGCTGCTG TTACTTCTGC TGCTGGTGGT GGTGGTGGTT CAGAAGCATC AACATCAGCT 
CCTGAAACGC CAGTCTCCTC TTACCCTCCA TGGATGAAGA GTCCAGAGCG GGGCGGCATC 
TCTTTTTCCC CAGTGAATTC AAATCTGAGA GACCTCACTC CTTCACACAC CCTGGAGATG 
GGGGCGTACA GGCCGGATTC AACCTCCAGT GGCCCTTTTA GTGAAGCCGC ACCCTTCTTT 
TCTTGCAATG AGGAGAGCAG TGGTGTGACC TTTACCCGCT CGCTAAGTGG AGATGGCACA 
GGGGAGGGAA GCACTGCAAA AAACCCACAG AAGAAAAAGG TCTCTCTGTT GGAGTACAGA 
AAGCGTCAGC GTGAGGCACG GCGGAGCGGC TCCAAAGGCG AGTGTAGCTC CCCTGTTTCC 
ACAGCACCAC CAGTAGACGT GTTCCCTGTG GCGGTGGAAA TGGCTGTTGA TCCTCCTGCT 
CCTGCAGCAA CACCGACCCC CAGGACCCCG CAGCCTAGTG AGGATCCAGA CGCCCCTCAG 
GGGGAGAGAG ATGGAGAAGG CCAGTGGACA TCTTCCACAT CAGTAGAGCA GGCCAGGGAG 
CGGGGCTACC ACAGGGCCTT ATTGCTGAGT GATCATCGCA AAGATACAGA TGGTGGAGAG 
TCAGAAGGTG GTGATTCCCA AGTGAAGGAC TGTTCTTCTC CCAAGAGCTG CAAGAGCCCC 
TCGACACATG CACCTTGTTC TCCAGCAGCC CAGGCTGTGT CTCGGCCATC AAAGGATGAA 
GATGAAGCAC AGACTCGTGG TCCTGCTCAG CCTCAGGCAG TGTCCGTCCA GCCGTCCAGC 
GCTAAGACTC CCAGCTCAAA ACCAGCTGCT CTGACGCCCA GCAAGCTCCA CTGTGGGCCC 
TCAGGCGCCT CTCAGAGTCA CTACGCGGGA CCTTCGCTCA TGCACTCTCC CAAAGCGCAA 
CCCCAAGGAT CACCTTTCCG TGGCCAGCGA GCCTTTCTGA CAGCTCAGCC TCAGAACCAG 
CCTCAACCAC AAGCTACATC TGGCCCTGCA ACTTTCCCTC AGTATAACCC TCAAAATGCA 
CCTCCACCTC CGCCCCCTCC TCCCCCAGCT CCACCTACAT CTGCTCCTTA CTTCCCCACC 
CAGCCTACTG CTGCTGCTGC TCCCTTTCCT GCATTTAAGC CCACCGTAGC CTCGCCCTTT 
CCTCCTGGTT CCCAGCCTCT ACTTCAGACC CATCATGCAC TGCATTACCA AAGCAGTGCT 
GCTCCTCCTC CTCCACCACC TCCTCCCCCT CCCCACCCGC AGCCTGGCCC CACCTTACTG 
CATGTCAATC TGCAACCTCC TTCACTGCAG CAGCATCAGC TCCTGCTGAG CTCCGCCCCA 
CCTCCGCCTC CTCCTCCACC TCCTCAGACA CAGAGTTCCC AGCAGCCCCC GCCTAACTCC 
GGCACGCTCC TGTCAATCAA ACAAGGACCA CACCCACCCC TTCCACCCCC TCCTCCAGCT 
CCCTCCACTA ACGCACCACC GCACCCGTTC CAAAACATCA GTGGCTTTCA AACCACTCTA 
CTCCACCAGT CTGCACCAGC CAACCCCTCA GTAACCGCAT CTACTTATCA ACAAACTGTA 
TTACCCCCTC CTCCTCCACC TCCCCCCCAA CAAACTCCTC CCACACAGAC CCCGCCCAAT 
CCGAGCATCC CTCAGATCGC AGGTGGCAAT CGAGGACCCG CTCCTTCCTC CGCCTCTTTC 
CACACCACCG GATACTTGGG CACAGGATGG CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_005164909.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164966.1 Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X4, mRNA.

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CloneID	ODa09484
Clone ID Related Accession (Same CDS sequence)	XM_005164909.4 , XM_005164909.3
Accession Version	XM_005164909.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	histone-lysine N-methyltransferase 2E isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164909.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACGGCCAACA CCTCGTATCT GGACATGGCT 
GCAGGCTCAG AACCAGAATC TGTGGAGGCG AGCTCTGTGG TAGTTGAGAA GTCCACCTAC 
CCTCACCAGA TTTACAGCAG CAGCTCCCAC CACTCCCACG GATACATCGG TCTGCCTTAC 
GCCGACCATA ATTATGGGGC GCGACCCCCA CCCACCCCGC CGGCGTCCCC ACCTCCCTCG 
GTGCTGATCC GGCCGGGCGA GAGCTTGTTT GTGTCCGGAA GCCGGCCTGG TGAGAACTTG 
TTTGTGCCGG GCGGGCAGGA CGAGGCGTCG CGTGGCACCA CGCTCAGCAC CTCGGAGGAC 
GGCAGCTACG GTGCTGACAT CACACGCTGC ATCTGTGGCT TCACCCACGA CGATGGCTAC 
ATGATCTGCT GCGACAAGTG CAGTGTGTGG CAGCACATAG ACTGCATGGG GATCGACAGG 
CAGCACATTC CCGAAACCTA CCTGTGTGAG CGCTGTCAGC CACGCACCCT GGACAGAGAG 
CACGCCATCC TCCTGCAAAC CAGGAAGAGA GAGAACATGT CTGATGGGGA CACCAGTGCT 
ACAGAAAGCG GAGATGAGGT TCCACTAGAG CTGTACACAG CGTTTCAGCA CACCCCTACC 
AGCATCACAC TCACAACAGC ACGTCTGGGT AACAAACAGG CTGATAAGAA ACGAAAGAAG 
AGCGGAGAGA AGGAGCCGCC GGCTACAGCC AGGGCCAAGA AGTCATTCCG TGAAGGCTCT 
AGAAAATCAT CTAGAGTAAA GGGCTCCGCT CCCGAGTGTG AGCCGATTGA TCCTCCCTCT 
CTATGGGAGA ATAAGATGAA GTCGTGGATG GAGCGCTACG AGGAGGCCAG CAGTAACCAG 
TACAGTGAAG AAGTGCGGCT ACTGCTGAGA GTCAAAGAAG CCCGAGATGG AAAAACCCTG 
GCCTACAATA CACACACCGC AGCCTTCAAG CCACCTGTAG AGAGTCAAGT ACAAAAAAAC 
AAGCGAATTC TGAAGGCTGT GCGGGATTTG GCTGCTGATT CATTAATAAT CGAGTACAGG 
GGGAAGGTCA TGCTAAGACA GCAGTTTGAG GCTAATGGCT ACTTCTTTAA GAGGCCATAC 
CCTTTTGTAT TGTTCTACTC TAAGTTTGAC GGTTTGGAAA TGTGCGTAGA CGCACGGAGC 
TTTGGGAATG AGGCCCGATT CATTCGGCGC TCCTGCACCC CTAACGCTGA AGTGCGGCAC 
GTGATTGAAG AGGGCATGCT GCATTTATAC ATTTACTCTC TGAGGTCCAT CTCCAAAGGG 
AGCGAGATCA CCATTGGCTT TGATTACGAC TACGGCAGCT GTAAATATAA GGTGGACTGT 
GCTTGTGTAA AAGGCAATCC GGAGTGTCCT GTGCTCAAGC ATAACCTGGA GCCCACAGAG 
AATCTAGGCT CTAGCACACG ACGGCGAGGC CGTAAGGACA AAGAACCATC GCGGGACGAG 
AGCGGACAAA ATCAGAACCT CACTATGGAC TGTGACGGGT CCAAGGGGAA AACGCTTAAT 
GACGCCAAAC AGAGAAAACT CTCTCCTCTC AGGCTCTCAA TATCTAACAA TCAGGGCAGC 
CCGGCAGAGG AGTCCCACCG TCACGACGCA GGGGCTTCCT GCTGCAGAGC TCTGAGAAAC 
AAGGAGACAC GGGAAGAGAG GAAGATGGAG GCCATTCTGC AGGCGTTTGC TCGTATGGAG 
AAGAGGGAAA AGCGGAGGGA GCAAGCGTTG GAGAGGATTG GCACCAAAGG GGAGGTTGGT 
GGACGCAGCG ATATAAAGGA GGAGGCACCT GCAACCCCTG AGGCTGAATC TCCTGCCGTG 
ATACAGCCGC TGCTAGAGGT AGTAAAGGAG GAGCCTGGTC TGAAACCGGC GAAGGTGAGC 
CGTAACAAAC AGAGAAAAAG CTTCTCCCGG AACCGGACGC ACATAGGACA GCAGAGGCGG 
CGAGCACGAA CTGTCAGCAC CTGCTCCGAC TTGCCCCCCA GTTCCCCTGG TGACACTTTA 
GAGCCCTTAA TGGCAGAGAC TCAAGATATA GAGATGCCTG TGGCCCCTGA AGCCGATGCT 
CCGCCAACGC AACCCCCTGA AACAAGCCCA CCCCACAGTG GCTCACCAGC CCCAACATGC 
CGCAGTGGAC AGAAGTACCC CAAAACTAAA AAGCACTTAG TGAGTGAGTG GATGGGTGTT 
GACAAACAGG AGCGGGTTGC GTCACGCACT CCGGAGCCTC CCCCTGAAAG GCCGTTGCGG 
ATCAGTAGTG ATCCTGAAGT CTTAGCCACA CAACTGAACT CTTTACCGGG CATGTCCTGC 
AGCTCACACG TCTACAGCAC GCCAAAACAC TACGTCCGCT TTTCTTCTCC TTTCCTGGCC 
AACCGTAGTC CCAGTACTCC TGGAGTGCCC ACGGGACGAC GGCGCTCGAG AGAGATGCCT 
GAAACGCCAA CTTCCACTGG CTCCTGCAAG AAGCGCTGGC TAAAGCAGGC TTTAGAAGAA 
GAAGGTTCGA CCAGCCCAGG TGGAGGACGA CCTTTTCTGC TCATACCCAG TGAGAGTCCT 
CTCAGTCCCT CTATAAACGG CGAGTCCTGC AGCCCTCTAC CCCTCAATGG AAGTTGCTCA 
TTACCAGAGT TGCCTACACC GTTGAAGAAG AGGCGCTTGT GTTCACTCGA TCCCTGCATG 
TCGGAGACTT CCACCCCATA TGGGTCTCCA TGTGCCACGC CAACCCGAAC AGAGTCAACA 
GAAGCACCGG GCACACCTTC GCTGCTAGCA ACGCCTCCTC GACCTCGACC AGAGGAGCCC 
AGTACCGAGC CCTCGACACC CTTGCAGATG CCCAACCACC CTCTGTTACA GGAGAGTGAA 
TCGTCAATGG ACAGTTCTCC AGATGGCAGC CGGAGGCCCA GTACACAAGA TGTTGAGCGA 
CCTCCTTCGC TGCTGGCATC TCCCAGCGTG AGAAATGTGA GCTCTGATAC GGCTCCACAG 
GAGTCTAGTA AATCTATGGG GTCTCTGAGT CCTCAGCCCT CCCACACGGA GCCCCAGGAT 
GCCGCAGTGG ATGAAGGCAT GGAGGTTGAT GCTGCTGCTG CTGTTACTTC TGCTGCTGGT 
GGTGGTGGTG GTTCAGAAGC ATCAACATCA GCTCCTGAAA CGCCAGTCTC CTCTTACCCT 
CCATGGATGA AGAGTCCAGA GCGGGGCGGC ATCTCTTTTT CCCCAGTGAA TTCAAATCTG 
AGAGACCTCA CTCCTTCACA CACCCTGGAG ATGGGGGCGT ACAGGCCGGA TTCAACCTCC 
AGTGGCCCTT TTAGTGAAGC CGCACCCTTC TTTTCTTGCA ATGAGGAGAG CAGTGGTGTG 
ACCTTTACCC GCTCGCTAAG TGGAGATGGC ACAGGGGAGG GAAGCACTGC AAAAAACCCA 
CAGAAGAAAA AGGTCTCTCT GTTGGAGTAC AGAAAGCGTC AGCGTGAGGC ACGGCGGAGC 
GGCTCCAAAG GCGAGTGTAG CTCCCCTGTT TCCACAGCAC CACCAGTAGA CGTGTTCCCT 
GTGGCGGTGG AAATGGCTGT TGATCCTCCT GCTCCTGCAG CAACACCGAC CCCCAGGACC 
CCGCAGCCTA GTGAGGATCC AGACGCCCCT CAGGGGGAGA GAGATGGAGA AGGCCAGTGG 
ACATCTTCCA CATCAGTAGA GCAGGCCAGG GAGCGGGGCT ACCACAGGGC CTTATTGCTG 
AGTGATCATC GCAAAGATAC AGATGGTGGA GAGTCAGAAG GTGGTGATTC CCAAGTGAAG 
GACTGTTCTT CTCCCAAGAG CTGCAAGAGC CCCTCGACAC ATGCACCTTG TTCTCCAGCA 
GCCCAGGCTG TGTCTCGGCC ATCAAAGGAT GAAGATGAAG CACAGACTCG TGGTCCTGCT 
CAGCCTCAGG CAGTGTCCGT CCAGCCGTCC AGCGCTAAGA CTCCCAGCTC AAAACCAGCT 
GCTCTGACGC CCAGCAAGCT CCACTGTGGG CCCTCAGGCG CCTCTCAGAG TCACTACGCG 
GGACCTTCGC TCATGCACTC TCCCAAAGCG CAACCCCAAG GATCACCTTT CCGTGGCCAG 
CGAGCCTTTC TGACAGCTCA GCCTCAGAAC CAGCCTCAAC CACAAGCTAC ATCTGGCCCT 
GCAACTTTCC CTCAGTATAA CCCTCAAAAT GCACCTCCAC CTCCGCCCCC TCCTCCCCCA 
GCTCCACCTA CATCTGCTCC TTACTTCCCC ACCCAGCCTA CTGCTGCTGC TGCTCCCTTT 
CCTGCATTTA AGCCCACCGT AGCCTCGCCC TTTCCTCCTG GTTCCCAGCC TCTACTTCAG 
ACCCATCATG CACTGCATTA CCAAAGCAGT GCTGCTCCTC CTCCTCCACC ACCTCCTCCC 
CCTCCCCACC CGCAGCCTGG CCCCACCTTA CTGCATGTCA ATCTGCAACC TCCTTCACTG 
CAGCAGCATC AGCTCCTGCT GAGCTCCGCC CCACCTCCGC CTCCTCCTCC ACCTCCTCAG 
ACACAGAGTT CCCAGCAGCC CCCGCCTAAC TCCGGCACGC TCCTGTCAAT CAAACAAGGA 
CCACACCCAC CCCTTCCACC CCCTCCTCCA GCTCCCTCCA CTAACGCACC ACCGCACCCG 
TTCCAAAACA TCAGTGGCTT TCAAACCACT CTACTCCACC AGTCTGCACC AGCCAACCCC 
TCAGTAACCG CATCTACTTA TCAACAAACT GTATTACCCC CTCCTCCTCC ACCTCCCCCC 
CAACAAACTC CTCCCACACA GACCCCGCCC AATCCGAGCA TCCCTCAGAT CGCAGGTGGC 
AATCGAGGAC CCGCTCCTTC CTCCGCCTCT TTCCACACCA CCGGATACTT GGGCACAGGA 
TGGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164966.1
CDS	1082..5950
Translation	MSIVIPVGVDTANTSYLDMAAGSEPESVEASSVVVEKSTYPHQIYSSSSHHSHGYIGLPYADHNYGARPPPTPPASPPPSVLIRPGESLFVSGSRPGENLFVPGGQDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQHIPETYLCERCQPRTLDREHAILLQTRKRENMSDGDTSATESGDEVPLELYTAFQHTPTSITLTTARLGNKQADKKRKKSGEKEPPATARAKKSFREGSRKSSRVKGSAPECEPIDPPSLWENKMKSWMERYEEASSNQYSEEVRLLLRVKEARDGKTLAYNTHTAAFKPPVESQVQKNKRILKAVRDLAADSLIIEYRGKVMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARSFGNEARFIRRSCTPNAEVRHVIEEGMLHLYIYSLRSISKGSEITIGFDYDYGSCKYKVDCACVKGNPECPVLKHNLEPTENLGSSTRRRGRKDKEPSRDESGQNQNLTMDCDGSKGKTLNDAKQRKLSPLRLSISNNQGSPAEESHRHDAGASCCRALRNKETREERKMEAILQAFARMEKREKRREQALERIGTKGEVGGRSDIKEEAPATPEAESPAVIQPLLEVVKEEPGLKPAKVSRNKQRKSFSRNRTHIGQQRRRARTVSTCSDLPPSSPGDTLEPLMAETQDIEMPVAPEADAPPTQPPETSPPHSGSPAPTCRSGQKYPKTKKHLVSEWMGVDKQERVASRTPEPPPERPLRISSDPEVLATQLNSLPGMSCSSHVYSTPKHYVRFSSPFLANRSPSTPGVPTGRRRSREMPETPTSTGSCKKRWLKQALEEEGSTSPGGGRPFLLIPSESPLSPSINGESCSPLPLNGSCSLPELPTPLKKRRLCSLDPCMSETSTPYGSPCATPTRTESTEAPGTPSLLATPPRPRPEEPSTEPSTPLQMPNHPLLQESESSMDSSPDGSRRPSTQDVERPPSLLASPSVRNVSSDTAPQESSKSMGSLSPQPSHTEPQDAAVDEGMEVDAAAAVTSAAGGGGGSEASTSAPETPVSSYPPWMKSPERGGISFSPVNSNLRDLTPSHTLEMGAYRPDSTSSGPFSEAAPFFSCNEESSGVTFTRSLSGDGTGEGSTAKNPQKKKVSLLEYRKRQREARRSGSKGECSSPVSTAPPVDVFPVAVEMAVDPPAPAATPTPRTPQPSEDPDAPQGERDGEGQWTSSTSVEQARERGYHRALLLSDHRKDTDGGESEGGDSQVKDCSSPKSCKSPSTHAPCSPAAQAVSRPSKDEDEAQTRGPAQPQAVSVQPSSAKTPSSKPAALTPSKLHCGPSGASQSHYAGPSLMHSPKAQPQGSPFRGQRAFLTAQPQNQPQPQATSGPATFPQYNPQNAPPPPPPPPPAPPTSAPYFPTQPTAAAAPFPAFKPTVASPFPPGSQPLLQTHHALHYQSSAAPPPPPPPPPPHPQPGPTLLHVNLQPPSLQQHQLLLSSAPPPPPPPPPQTQSSQQPPPNSGTLLSIKQGPHPPLPPPPPAPSTNAPPHPFQNISGFQTTLLHQSAPANPSVTASTYQQTVLPPPPPPPPQQTPPTQTPPNPSIPQIAGGNRGPAPSSASFHTTGYLGTGWH

Target ORF information:

RefSeq Version	XM_005164909.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164909.4

ORF Insert Sequence:

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACGGCCAACA CCTCGTATCT GGACATGGCT 
GCAGGCTCAG AACCAGAATC TGTGGAGGCG AGCTCTGTGG TAGTTGAGAA GTCCACCTAC 
CCTCACCAGA TTTACAGCAG CAGCTCCCAC CACTCCCACG GATACATCGG TCTGCCTTAC 
GCCGACCATA ATTATGGGGC GCGACCCCCA CCCACCCCGC CGGCGTCCCC ACCTCCCTCG 
GTGCTGATCC GGCCGGGCGA GAGCTTGTTT GTGTCCGGAA GCCGGCCTGG TGAGAACTTG 
TTTGTGCCGG GCGGGCAGGA CGAGGCGTCG CGTGGCACCA CGCTCAGCAC CTCGGAGGAC 
GGCAGCTACG GTGCTGACAT CACACGCTGC ATCTGTGGCT TCACCCACGA CGATGGCTAC 
ATGATCTGCT GCGACAAGTG CAGTGTGTGG CAGCACATAG ACTGCATGGG GATCGACAGG 
CAGCACATTC CCGAAACCTA CCTGTGTGAG CGCTGTCAGC CACGCACCCT GGACAGAGAG 
CACGCCATCC TCCTGCAAAC CAGGAAGAGA GAGAACATGT CTGATGGGGA CACCAGTGCT 
ACAGAAAGCG GAGATGAGGT TCCACTAGAG CTGTACACAG CGTTTCAGCA CACCCCTACC 
AGCATCACAC TCACAACAGC ACGTCTGGGT AACAAACAGG CTGATAAGAA ACGAAAGAAG 
AGCGGAGAGA AGGAGCCGCC GGCTACAGCC AGGGCCAAGA AGTCATTCCG TGAAGGCTCT 
AGAAAATCAT CTAGAGTAAA GGGCTCCGCT CCCGAGTGTG AGCCGATTGA TCCTCCCTCT 
CTATGGGAGA ATAAGATGAA GTCGTGGATG GAGCGCTACG AGGAGGCCAG CAGTAACCAG 
TACAGTGAAG AAGTGCGGCT ACTGCTGAGA GTCAAAGAAG CCCGAGATGG AAAAACCCTG 
GCCTACAATA CACACACCGC AGCCTTCAAG CCACCTGTAG AGAGTCAAGT ACAAAAAAAC 
AAGCGAATTC TGAAGGCTGT GCGGGATTTG GCTGCTGATT CATTAATAAT CGAGTACAGG 
GGGAAGGTCA TGCTAAGACA GCAGTTTGAG GCTAATGGCT ACTTCTTTAA GAGGCCATAC 
CCTTTTGTAT TGTTCTACTC TAAGTTTGAC GGTTTGGAAA TGTGCGTAGA CGCACGGAGC 
TTTGGGAATG AGGCCCGATT CATTCGGCGC TCCTGCACCC CTAACGCTGA AGTGCGGCAC 
GTGATTGAAG AGGGCATGCT GCATTTATAC ATTTACTCTC TGAGGTCCAT CTCCAAAGGG 
AGCGAGATCA CCATTGGCTT TGATTACGAC TACGGCAGCT GTAAATATAA GGTGGACTGT 
GCTTGTGTAA AAGGCAATCC GGAGTGTCCT GTGCTCAAGC ATAACCTGGA GCCCACAGAG 
AATCTAGGCT CTAGCACACG ACGGCGAGGC CGTAAGGACA AAGAACCATC GCGGGACGAG 
AGCGGACAAA ATCAGAACCT CACTATGGAC TGTGACGGGT CCAAGGGGAA AACGCTTAAT 
GACGCCAAAC AGAGAAAACT CTCTCCTCTC AGGCTCTCAA TATCTAACAA TCAGGGCAGC 
CCGGCAGAGG AGTCCCACCG TCACGACGCA GGGGCTTCCT GCTGCAGAGC TCTGAGAAAC 
AAGGAGACAC GGGAAGAGAG GAAGATGGAG GCCATTCTGC AGGCGTTTGC TCGTATGGAG 
AAGAGGGAAA AGCGGAGGGA GCAAGCGTTG GAGAGGATTG GCACCAAAGG GGAGGTTGGT 
GGACGCAGCG ATATAAAGGA GGAGGCACCT GCAACCCCTG AGGCTGAATC TCCTGCCGTG 
ATACAGCCGC TGCTAGAGGT AGTAAAGGAG GAGCCTGGTC TGAAACCGGC GAAGGTGAGC 
CGTAACAAAC AGAGAAAAAG CTTCTCCCGG AACCGGACGC ACATAGGACA GCAGAGGCGG 
CGAGCACGAA CTGTCAGCAC CTGCTCCGAC TTGCCCCCCA GTTCCCCTGG TGACACTTTA 
GAGCCCTTAA TGGCAGAGAC TCAAGATATA GAGATGCCTG TGGCCCCTGA AGCCGATGCT 
CCGCCAACGC AACCCCCTGA AACAAGCCCA CCCCACAGTG GCTCACCAGC CCCAACATGC 
CGCAGTGGAC AGAAGTACCC CAAAACTAAA AAGCACTTAG TGAGTGAGTG GATGGGTGTT 
GACAAACAGG AGCGGGTTGC GTCACGCACT CCGGAGCCTC CCCCTGAAAG GCCGTTGCGG 
ATCAGTAGTG ATCCTGAAGT CTTAGCCACA CAACTGAACT CTTTACCGGG CATGTCCTGC 
AGCTCACACG TCTACAGCAC GCCAAAACAC TACGTCCGCT TTTCTTCTCC TTTCCTGGCC 
AACCGTAGTC CCAGTACTCC TGGAGTGCCC ACGGGACGAC GGCGCTCGAG AGAGATGCCT 
GAAACGCCAA CTTCCACTGG CTCCTGCAAG AAGCGCTGGC TAAAGCAGGC TTTAGAAGAA 
GAAGGTTCGA CCAGCCCAGG TGGAGGACGA CCTTTTCTGC TCATACCCAG TGAGAGTCCT 
CTCAGTCCCT CTATAAACGG CGAGTCCTGC AGCCCTCTAC CCCTCAATGG AAGTTGCTCA 
TTACCAGAGT TGCCTACACC GTTGAAGAAG AGGCGCTTGT GTTCACTCGA TCCCTGCATG 
TCGGAGACTT CCACCCCATA TGGGTCTCCA TGTGCCACGC CAACCCGAAC AGAGTCAACA 
GAAGCACCGG GCACACCTTC GCTGCTAGCA ACGCCTCCTC GACCTCGACC AGAGGAGCCC 
AGTACCGAGC CCTCGACACC CTTGCAGATG CCCAACCACC CTCTGTTACA GGAGAGTGAA 
TCGTCAATGG ACAGTTCTCC AGATGGCAGC CGGAGGCCCA GTACACAAGA TGTTGAGCGA 
CCTCCTTCGC TGCTGGCATC TCCCAGCGTG AGAAATGTGA GCTCTGATAC GGCTCCACAG 
GAGTCTAGTA AATCTATGGG GTCTCTGAGT CCTCAGCCCT CCCACACGGA GCCCCAGGAT 
GCCGCAGTGG ATGAAGGCAT GGAGGTTGAT GCTGCTGCTG CTGTTACTTC TGCTGCTGGT 
GGTGGTGGTG GTTCAGAAGC ATCAACATCA GCTCCTGAAA CGCCAGTCTC CTCTTACCCT 
CCATGGATGA AGAGTCCAGA GCGGGGCGGC ATCTCTTTTT CCCCAGTGAA TTCAAATCTG 
AGAGACCTCA CTCCTTCACA CACCCTGGAG ATGGGGGCGT ACAGGCCGGA TTCAACCTCC 
AGTGGCCCTT TTAGTGAAGC CGCACCCTTC TTTTCTTGCA ATGAGGAGAG CAGTGGTGTG 
ACCTTTACCC GCTCGCTAAG TGGAGATGGC ACAGGGGAGG GAAGCACTGC AAAAAACCCA 
CAGAAGAAAA AGGTCTCTCT GTTGGAGTAC AGAAAGCGTC AGCGTGAGGC ACGGCGGAGC 
GGCTCCAAAG GCGAGTGTAG CTCCCCTGTT TCCACAGCAC CACCAGTAGA CGTGTTCCCT 
GTGGCGGTGG AAATGGCTGT TGATCCTCCT GCTCCTGCAG CAACACCGAC CCCCAGGACC 
CCGCAGCCTA GTGAGGATCC AGACGCCCCT CAGGGGGAGA GAGATGGAGA AGGCCAGTGG 
ACATCTTCCA CATCAGTAGA GCAGGCCAGG GAGCGGGGCT ACCACAGGGC CTTATTGCTG 
AGTGATCATC GCAAAGATAC AGATGGTGGA GAGTCAGAAG GTGGTGATTC CCAAGTGAAG 
GACTGTTCTT CTCCCAAGAG CTGCAAGAGC CCCTCGACAC ATGCACCTTG TTCTCCAGCA 
GCCCAGGCTG TGTCTCGGCC ATCAAAGGAT GAAGATGAAG CACAGACTCG TGGTCCTGCT 
CAGCCTCAGG CAGTGTCCGT CCAGCCGTCC AGCGCTAAGA CTCCCAGCTC AAAACCAGCT 
GCTCTGACGC CCAGCAAGCT CCACTGTGGG CCCTCAGGCG CCTCTCAGAG TCACTACGCG 
GGACCTTCGC TCATGCACTC TCCCAAAGCG CAACCCCAAG GATCACCTTT CCGTGGCCAG 
CGAGCCTTTC TGACAGCTCA GCCTCAGAAC CAGCCTCAAC CACAAGCTAC ATCTGGCCCT 
GCAACTTTCC CTCAGTATAA CCCTCAAAAT GCACCTCCAC CTCCGCCCCC TCCTCCCCCA 
GCTCCACCTA CATCTGCTCC TTACTTCCCC ACCCAGCCTA CTGCTGCTGC TGCTCCCTTT 
CCTGCATTTA AGCCCACCGT AGCCTCGCCC TTTCCTCCTG GTTCCCAGCC TCTACTTCAG 
ACCCATCATG CACTGCATTA CCAAAGCAGT GCTGCTCCTC CTCCTCCACC ACCTCCTCCC 
CCTCCCCACC CGCAGCCTGG CCCCACCTTA CTGCATGTCA ATCTGCAACC TCCTTCACTG 
CAGCAGCATC AGCTCCTGCT GAGCTCCGCC CCACCTCCGC CTCCTCCTCC ACCTCCTCAG 
ACACAGAGTT CCCAGCAGCC CCCGCCTAAC TCCGGCACGC TCCTGTCAAT CAAACAAGGA 
CCACACCCAC CCCTTCCACC CCCTCCTCCA GCTCCCTCCA CTAACGCACC ACCGCACCCG 
TTCCAAAACA TCAGTGGCTT TCAAACCACT CTACTCCACC AGTCTGCACC AGCCAACCCC 
TCAGTAACCG CATCTACTTA TCAACAAACT GTATTACCCC CTCCTCCTCC ACCTCCCCCC 
CAACAAACTC CTCCCACACA GACCCCGCCC AATCCGAGCA TCCCTCAGAT CGCAGGTGGC 
AATCGAGGAC CCGCTCCTTC CTCCGCCTCT TTCCACACCA CCGGATACTT GGGCACAGGA 
TGGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_005164909.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164966.1 Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X5, mRNA.

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CloneID	ODa09484
Clone ID Related Accession (Same CDS sequence)	XM_005164909.4 , XM_005164909.3
Accession Version	XM_005164909.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	histone-lysine N-methyltransferase 2E isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164909.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACGGCCAACA CCTCGTATCT GGACATGGCT 
GCAGGCTCAG AACCAGAATC TGTGGAGGCG AGCTCTGTGG TAGTTGAGAA GTCCACCTAC 
CCTCACCAGA TTTACAGCAG CAGCTCCCAC CACTCCCACG GATACATCGG TCTGCCTTAC 
GCCGACCATA ATTATGGGGC GCGACCCCCA CCCACCCCGC CGGCGTCCCC ACCTCCCTCG 
GTGCTGATCC GGCCGGGCGA GAGCTTGTTT GTGTCCGGAA GCCGGCCTGG TGAGAACTTG 
TTTGTGCCGG GCGGGCAGGA CGAGGCGTCG CGTGGCACCA CGCTCAGCAC CTCGGAGGAC 
GGCAGCTACG GTGCTGACAT CACACGCTGC ATCTGTGGCT TCACCCACGA CGATGGCTAC 
ATGATCTGCT GCGACAAGTG CAGTGTGTGG CAGCACATAG ACTGCATGGG GATCGACAGG 
CAGCACATTC CCGAAACCTA CCTGTGTGAG CGCTGTCAGC CACGCACCCT GGACAGAGAG 
CACGCCATCC TCCTGCAAAC CAGGAAGAGA GAGAACATGT CTGATGGGGA CACCAGTGCT 
ACAGAAAGCG GAGATGAGGT TCCACTAGAG CTGTACACAG CGTTTCAGCA CACCCCTACC 
AGCATCACAC TCACAACAGC ACGTCTGGGT AACAAACAGG CTGATAAGAA ACGAAAGAAG 
AGCGGAGAGA AGGAGCCGCC GGCTACAGCC AGGGCCAAGA AGTCATTCCG TGAAGGCTCT 
AGAAAATCAT CTAGAGTAAA GGGCTCCGCT CCCGAGTGTG AGCCGATTGA TCCTCCCTCT 
CTATGGGAGA ATAAGATGAA GTCGTGGATG GAGCGCTACG AGGAGGCCAG CAGTAACCAG 
TACAGTGAAG AAGTGCGGCT ACTGCTGAGA GTCAAAGAAG CCCGAGATGG AAAAACCCTG 
GCCTACAATA CACACACCGC AGCCTTCAAG CCACCTGTAG AGAGTCAAGT ACAAAAAAAC 
AAGCGAATTC TGAAGGCTGT GCGGGATTTG GCTGCTGATT CATTAATAAT CGAGTACAGG 
GGGAAGGTCA TGCTAAGACA GCAGTTTGAG GCTAATGGCT ACTTCTTTAA GAGGCCATAC 
CCTTTTGTAT TGTTCTACTC TAAGTTTGAC GGTTTGGAAA TGTGCGTAGA CGCACGGAGC 
TTTGGGAATG AGGCCCGATT CATTCGGCGC TCCTGCACCC CTAACGCTGA AGTGCGGCAC 
GTGATTGAAG AGGGCATGCT GCATTTATAC ATTTACTCTC TGAGGTCCAT CTCCAAAGGG 
AGCGAGATCA CCATTGGCTT TGATTACGAC TACGGCAGCT GTAAATATAA GGTGGACTGT 
GCTTGTGTAA AAGGCAATCC GGAGTGTCCT GTGCTCAAGC ATAACCTGGA GCCCACAGAG 
AATCTAGGCT CTAGCACACG ACGGCGAGGC CGTAAGGACA AAGAACCATC GCGGGACGAG 
AGCGGACAAA ATCAGAACCT CACTATGGAC TGTGACGGGT CCAAGGGGAA AACGCTTAAT 
GACGCCAAAC AGAGAAAACT CTCTCCTCTC AGGCTCTCAA TATCTAACAA TCAGGGCAGC 
CCGGCAGAGG AGTCCCACCG TCACGACGCA GGGGCTTCCT GCTGCAGAGC TCTGAGAAAC 
AAGGAGACAC GGGAAGAGAG GAAGATGGAG GCCATTCTGC AGGCGTTTGC TCGTATGGAG 
AAGAGGGAAA AGCGGAGGGA GCAAGCGTTG GAGAGGATTG GCACCAAAGG GGAGGTTGGT 
GGACGCAGCG ATATAAAGGA GGAGGCACCT GCAACCCCTG AGGCTGAATC TCCTGCCGTG 
ATACAGCCGC TGCTAGAGGT AGTAAAGGAG GAGCCTGGTC TGAAACCGGC GAAGGTGAGC 
CGTAACAAAC AGAGAAAAAG CTTCTCCCGG AACCGGACGC ACATAGGACA GCAGAGGCGG 
CGAGCACGAA CTGTCAGCAC CTGCTCCGAC TTGCCCCCCA GTTCCCCTGG TGACACTTTA 
GAGCCCTTAA TGGCAGAGAC TCAAGATATA GAGATGCCTG TGGCCCCTGA AGCCGATGCT 
CCGCCAACGC AACCCCCTGA AACAAGCCCA CCCCACAGTG GCTCACCAGC CCCAACATGC 
CGCAGTGGAC AGAAGTACCC CAAAACTAAA AAGCACTTAG TGAGTGAGTG GATGGGTGTT 
GACAAACAGG AGCGGGTTGC GTCACGCACT CCGGAGCCTC CCCCTGAAAG GCCGTTGCGG 
ATCAGTAGTG ATCCTGAAGT CTTAGCCACA CAACTGAACT CTTTACCGGG CATGTCCTGC 
AGCTCACACG TCTACAGCAC GCCAAAACAC TACGTCCGCT TTTCTTCTCC TTTCCTGGCC 
AACCGTAGTC CCAGTACTCC TGGAGTGCCC ACGGGACGAC GGCGCTCGAG AGAGATGCCT 
GAAACGCCAA CTTCCACTGG CTCCTGCAAG AAGCGCTGGC TAAAGCAGGC TTTAGAAGAA 
GAAGGTTCGA CCAGCCCAGG TGGAGGACGA CCTTTTCTGC TCATACCCAG TGAGAGTCCT 
CTCAGTCCCT CTATAAACGG CGAGTCCTGC AGCCCTCTAC CCCTCAATGG AAGTTGCTCA 
TTACCAGAGT TGCCTACACC GTTGAAGAAG AGGCGCTTGT GTTCACTCGA TCCCTGCATG 
TCGGAGACTT CCACCCCATA TGGGTCTCCA TGTGCCACGC CAACCCGAAC AGAGTCAACA 
GAAGCACCGG GCACACCTTC GCTGCTAGCA ACGCCTCCTC GACCTCGACC AGAGGAGCCC 
AGTACCGAGC CCTCGACACC CTTGCAGATG CCCAACCACC CTCTGTTACA GGAGAGTGAA 
TCGTCAATGG ACAGTTCTCC AGATGGCAGC CGGAGGCCCA GTACACAAGA TGTTGAGCGA 
CCTCCTTCGC TGCTGGCATC TCCCAGCGTG AGAAATGTGA GCTCTGATAC GGCTCCACAG 
GAGTCTAGTA AATCTATGGG GTCTCTGAGT CCTCAGCCCT CCCACACGGA GCCCCAGGAT 
GCCGCAGTGG ATGAAGGCAT GGAGGTTGAT GCTGCTGCTG CTGTTACTTC TGCTGCTGGT 
GGTGGTGGTG GTTCAGAAGC ATCAACATCA GCTCCTGAAA CGCCAGTCTC CTCTTACCCT 
CCATGGATGA AGAGTCCAGA GCGGGGCGGC ATCTCTTTTT CCCCAGTGAA TTCAAATCTG 
AGAGACCTCA CTCCTTCACA CACCCTGGAG ATGGGGGCGT ACAGGCCGGA TTCAACCTCC 
AGTGGCCCTT TTAGTGAAGC CGCACCCTTC TTTTCTTGCA ATGAGGAGAG CAGTGGTGTG 
ACCTTTACCC GCTCGCTAAG TGGAGATGGC ACAGGGGAGG GAAGCACTGC AAAAAACCCA 
CAGAAGAAAA AGGTCTCTCT GTTGGAGTAC AGAAAGCGTC AGCGTGAGGC ACGGCGGAGC 
GGCTCCAAAG GCGAGTGTAG CTCCCCTGTT TCCACAGCAC CACCAGTAGA CGTGTTCCCT 
GTGGCGGTGG AAATGGCTGT TGATCCTCCT GCTCCTGCAG CAACACCGAC CCCCAGGACC 
CCGCAGCCTA GTGAGGATCC AGACGCCCCT CAGGGGGAGA GAGATGGAGA AGGCCAGTGG 
ACATCTTCCA CATCAGTAGA GCAGGCCAGG GAGCGGGGCT ACCACAGGGC CTTATTGCTG 
AGTGATCATC GCAAAGATAC AGATGGTGGA GAGTCAGAAG GTGGTGATTC CCAAGTGAAG 
GACTGTTCTT CTCCCAAGAG CTGCAAGAGC CCCTCGACAC ATGCACCTTG TTCTCCAGCA 
GCCCAGGCTG TGTCTCGGCC ATCAAAGGAT GAAGATGAAG CACAGACTCG TGGTCCTGCT 
CAGCCTCAGG CAGTGTCCGT CCAGCCGTCC AGCGCTAAGA CTCCCAGCTC AAAACCAGCT 
GCTCTGACGC CCAGCAAGCT CCACTGTGGG CCCTCAGGCG CCTCTCAGAG TCACTACGCG 
GGACCTTCGC TCATGCACTC TCCCAAAGCG CAACCCCAAG GATCACCTTT CCGTGGCCAG 
CGAGCCTTTC TGACAGCTCA GCCTCAGAAC CAGCCTCAAC CACAAGCTAC ATCTGGCCCT 
GCAACTTTCC CTCAGTATAA CCCTCAAAAT GCACCTCCAC CTCCGCCCCC TCCTCCCCCA 
GCTCCACCTA CATCTGCTCC TTACTTCCCC ACCCAGCCTA CTGCTGCTGC TGCTCCCTTT 
CCTGCATTTA AGCCCACCGT AGCCTCGCCC TTTCCTCCTG GTTCCCAGCC TCTACTTCAG 
ACCCATCATG CACTGCATTA CCAAAGCAGT GCTGCTCCTC CTCCTCCACC ACCTCCTCCC 
CCTCCCCACC CGCAGCCTGG CCCCACCTTA CTGCATGTCA ATCTGCAACC TCCTTCACTG 
CAGCAGCATC AGCTCCTGCT GAGCTCCGCC CCACCTCCGC CTCCTCCTCC ACCTCCTCAG 
ACACAGAGTT CCCAGCAGCC CCCGCCTAAC TCCGGCACGC TCCTGTCAAT CAAACAAGGA 
CCACACCCAC CCCTTCCACC CCCTCCTCCA GCTCCCTCCA CTAACGCACC ACCGCACCCG 
TTCCAAAACA TCAGTGGCTT TCAAACCACT CTACTCCACC AGTCTGCACC AGCCAACCCC 
TCAGTAACCG CATCTACTTA TCAACAAACT GTATTACCCC CTCCTCCTCC ACCTCCCCCC 
CAACAAACTC CTCCCACACA GACCCCGCCC AATCCGAGCA TCCCTCAGAT CGCAGGTGGC 
AATCGAGGAC CCGCTCCTTC CTCCGCCTCT TTCCACACCA CCGGATACTT GGGCACAGGA 
TGGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164966.1
CDS	1144..6012
Translation	MSIVIPVGVDTANTSYLDMAAGSEPESVEASSVVVEKSTYPHQIYSSSSHHSHGYIGLPYADHNYGARPPPTPPASPPPSVLIRPGESLFVSGSRPGENLFVPGGQDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQHIPETYLCERCQPRTLDREHAILLQTRKRENMSDGDTSATESGDEVPLELYTAFQHTPTSITLTTARLGNKQADKKRKKSGEKEPPATARAKKSFREGSRKSSRVKGSAPECEPIDPPSLWENKMKSWMERYEEASSNQYSEEVRLLLRVKEARDGKTLAYNTHTAAFKPPVESQVQKNKRILKAVRDLAADSLIIEYRGKVMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARSFGNEARFIRRSCTPNAEVRHVIEEGMLHLYIYSLRSISKGSEITIGFDYDYGSCKYKVDCACVKGNPECPVLKHNLEPTENLGSSTRRRGRKDKEPSRDESGQNQNLTMDCDGSKGKTLNDAKQRKLSPLRLSISNNQGSPAEESHRHDAGASCCRALRNKETREERKMEAILQAFARMEKREKRREQALERIGTKGEVGGRSDIKEEAPATPEAESPAVIQPLLEVVKEEPGLKPAKVSRNKQRKSFSRNRTHIGQQRRRARTVSTCSDLPPSSPGDTLEPLMAETQDIEMPVAPEADAPPTQPPETSPPHSGSPAPTCRSGQKYPKTKKHLVSEWMGVDKQERVASRTPEPPPERPLRISSDPEVLATQLNSLPGMSCSSHVYSTPKHYVRFSSPFLANRSPSTPGVPTGRRRSREMPETPTSTGSCKKRWLKQALEEEGSTSPGGGRPFLLIPSESPLSPSINGESCSPLPLNGSCSLPELPTPLKKRRLCSLDPCMSETSTPYGSPCATPTRTESTEAPGTPSLLATPPRPRPEEPSTEPSTPLQMPNHPLLQESESSMDSSPDGSRRPSTQDVERPPSLLASPSVRNVSSDTAPQESSKSMGSLSPQPSHTEPQDAAVDEGMEVDAAAAVTSAAGGGGGSEASTSAPETPVSSYPPWMKSPERGGISFSPVNSNLRDLTPSHTLEMGAYRPDSTSSGPFSEAAPFFSCNEESSGVTFTRSLSGDGTGEGSTAKNPQKKKVSLLEYRKRQREARRSGSKGECSSPVSTAPPVDVFPVAVEMAVDPPAPAATPTPRTPQPSEDPDAPQGERDGEGQWTSSTSVEQARERGYHRALLLSDHRKDTDGGESEGGDSQVKDCSSPKSCKSPSTHAPCSPAAQAVSRPSKDEDEAQTRGPAQPQAVSVQPSSAKTPSSKPAALTPSKLHCGPSGASQSHYAGPSLMHSPKAQPQGSPFRGQRAFLTAQPQNQPQPQATSGPATFPQYNPQNAPPPPPPPPPAPPTSAPYFPTQPTAAAAPFPAFKPTVASPFPPGSQPLLQTHHALHYQSSAAPPPPPPPPPPHPQPGPTLLHVNLQPPSLQQHQLLLSSAPPPPPPPPPQTQSSQQPPPNSGTLLSIKQGPHPPLPPPPPAPSTNAPPHPFQNISGFQTTLLHQSAPANPSVTASTYQQTVLPPPPPPPPQQTPPTQTPPNPSIPQIAGGNRGPAPSSASFHTTGYLGTGWH

Target ORF information:

RefSeq Version	XM_005164909.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164909.3

ORF Insert Sequence:

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACGGCCAACA CCTCGTATCT GGACATGGCT 
GCAGGCTCAG AACCAGAATC TGTGGAGGCG AGCTCTGTGG TAGTTGAGAA GTCCACCTAC 
CCTCACCAGA TTTACAGCAG CAGCTCCCAC CACTCCCACG GATACATCGG TCTGCCTTAC 
GCCGACCATA ATTATGGGGC GCGACCCCCA CCCACCCCGC CGGCGTCCCC ACCTCCCTCG 
GTGCTGATCC GGCCGGGCGA GAGCTTGTTT GTGTCCGGAA GCCGGCCTGG TGAGAACTTG 
TTTGTGCCGG GCGGGCAGGA CGAGGCGTCG CGTGGCACCA CGCTCAGCAC CTCGGAGGAC 
GGCAGCTACG GTGCTGACAT CACACGCTGC ATCTGTGGCT TCACCCACGA CGATGGCTAC 
ATGATCTGCT GCGACAAGTG CAGTGTGTGG CAGCACATAG ACTGCATGGG GATCGACAGG 
CAGCACATTC CCGAAACCTA CCTGTGTGAG CGCTGTCAGC CACGCACCCT GGACAGAGAG 
CACGCCATCC TCCTGCAAAC CAGGAAGAGA GAGAACATGT CTGATGGGGA CACCAGTGCT 
ACAGAAAGCG GAGATGAGGT TCCACTAGAG CTGTACACAG CGTTTCAGCA CACCCCTACC 
AGCATCACAC TCACAACAGC ACGTCTGGGT AACAAACAGG CTGATAAGAA ACGAAAGAAG 
AGCGGAGAGA AGGAGCCGCC GGCTACAGCC AGGGCCAAGA AGTCATTCCG TGAAGGCTCT 
AGAAAATCAT CTAGAGTAAA GGGCTCCGCT CCCGAGTGTG AGCCGATTGA TCCTCCCTCT 
CTATGGGAGA ATAAGATGAA GTCGTGGATG GAGCGCTACG AGGAGGCCAG CAGTAACCAG 
TACAGTGAAG AAGTGCGGCT ACTGCTGAGA GTCAAAGAAG CCCGAGATGG AAAAACCCTG 
GCCTACAATA CACACACCGC AGCCTTCAAG CCACCTGTAG AGAGTCAAGT ACAAAAAAAC 
AAGCGAATTC TGAAGGCTGT GCGGGATTTG GCTGCTGATT CATTAATAAT CGAGTACAGG 
GGGAAGGTCA TGCTAAGACA GCAGTTTGAG GCTAATGGCT ACTTCTTTAA GAGGCCATAC 
CCTTTTGTAT TGTTCTACTC TAAGTTTGAC GGTTTGGAAA TGTGCGTAGA CGCACGGAGC 
TTTGGGAATG AGGCCCGATT CATTCGGCGC TCCTGCACCC CTAACGCTGA AGTGCGGCAC 
GTGATTGAAG AGGGCATGCT GCATTTATAC ATTTACTCTC TGAGGTCCAT CTCCAAAGGG 
AGCGAGATCA CCATTGGCTT TGATTACGAC TACGGCAGCT GTAAATATAA GGTGGACTGT 
GCTTGTGTAA AAGGCAATCC GGAGTGTCCT GTGCTCAAGC ATAACCTGGA GCCCACAGAG 
AATCTAGGCT CTAGCACACG ACGGCGAGGC CGTAAGGACA AAGAACCATC GCGGGACGAG 
AGCGGACAAA ATCAGAACCT CACTATGGAC TGTGACGGGT CCAAGGGGAA AACGCTTAAT 
GACGCCAAAC AGAGAAAACT CTCTCCTCTC AGGCTCTCAA TATCTAACAA TCAGGGCAGC 
CCGGCAGAGG AGTCCCACCG TCACGACGCA GGGGCTTCCT GCTGCAGAGC TCTGAGAAAC 
AAGGAGACAC GGGAAGAGAG GAAGATGGAG GCCATTCTGC AGGCGTTTGC TCGTATGGAG 
AAGAGGGAAA AGCGGAGGGA GCAAGCGTTG GAGAGGATTG GCACCAAAGG GGAGGTTGGT 
GGACGCAGCG ATATAAAGGA GGAGGCACCT GCAACCCCTG AGGCTGAATC TCCTGCCGTG 
ATACAGCCGC TGCTAGAGGT AGTAAAGGAG GAGCCTGGTC TGAAACCGGC GAAGGTGAGC 
CGTAACAAAC AGAGAAAAAG CTTCTCCCGG AACCGGACGC ACATAGGACA GCAGAGGCGG 
CGAGCACGAA CTGTCAGCAC CTGCTCCGAC TTGCCCCCCA GTTCCCCTGG TGACACTTTA 
GAGCCCTTAA TGGCAGAGAC TCAAGATATA GAGATGCCTG TGGCCCCTGA AGCCGATGCT 
CCGCCAACGC AACCCCCTGA AACAAGCCCA CCCCACAGTG GCTCACCAGC CCCAACATGC 
CGCAGTGGAC AGAAGTACCC CAAAACTAAA AAGCACTTAG TGAGTGAGTG GATGGGTGTT 
GACAAACAGG AGCGGGTTGC GTCACGCACT CCGGAGCCTC CCCCTGAAAG GCCGTTGCGG 
ATCAGTAGTG ATCCTGAAGT CTTAGCCACA CAACTGAACT CTTTACCGGG CATGTCCTGC 
AGCTCACACG TCTACAGCAC GCCAAAACAC TACGTCCGCT TTTCTTCTCC TTTCCTGGCC 
AACCGTAGTC CCAGTACTCC TGGAGTGCCC ACGGGACGAC GGCGCTCGAG AGAGATGCCT 
GAAACGCCAA CTTCCACTGG CTCCTGCAAG AAGCGCTGGC TAAAGCAGGC TTTAGAAGAA 
GAAGGTTCGA CCAGCCCAGG TGGAGGACGA CCTTTTCTGC TCATACCCAG TGAGAGTCCT 
CTCAGTCCCT CTATAAACGG CGAGTCCTGC AGCCCTCTAC CCCTCAATGG AAGTTGCTCA 
TTACCAGAGT TGCCTACACC GTTGAAGAAG AGGCGCTTGT GTTCACTCGA TCCCTGCATG 
TCGGAGACTT CCACCCCATA TGGGTCTCCA TGTGCCACGC CAACCCGAAC AGAGTCAACA 
GAAGCACCGG GCACACCTTC GCTGCTAGCA ACGCCTCCTC GACCTCGACC AGAGGAGCCC 
AGTACCGAGC CCTCGACACC CTTGCAGATG CCCAACCACC CTCTGTTACA GGAGAGTGAA 
TCGTCAATGG ACAGTTCTCC AGATGGCAGC CGGAGGCCCA GTACACAAGA TGTTGAGCGA 
CCTCCTTCGC TGCTGGCATC TCCCAGCGTG AGAAATGTGA GCTCTGATAC GGCTCCACAG 
GAGTCTAGTA AATCTATGGG GTCTCTGAGT CCTCAGCCCT CCCACACGGA GCCCCAGGAT 
GCCGCAGTGG ATGAAGGCAT GGAGGTTGAT GCTGCTGCTG CTGTTACTTC TGCTGCTGGT 
GGTGGTGGTG GTTCAGAAGC ATCAACATCA GCTCCTGAAA CGCCAGTCTC CTCTTACCCT 
CCATGGATGA AGAGTCCAGA GCGGGGCGGC ATCTCTTTTT CCCCAGTGAA TTCAAATCTG 
AGAGACCTCA CTCCTTCACA CACCCTGGAG ATGGGGGCGT ACAGGCCGGA TTCAACCTCC 
AGTGGCCCTT TTAGTGAAGC CGCACCCTTC TTTTCTTGCA ATGAGGAGAG CAGTGGTGTG 
ACCTTTACCC GCTCGCTAAG TGGAGATGGC ACAGGGGAGG GAAGCACTGC AAAAAACCCA 
CAGAAGAAAA AGGTCTCTCT GTTGGAGTAC AGAAAGCGTC AGCGTGAGGC ACGGCGGAGC 
GGCTCCAAAG GCGAGTGTAG CTCCCCTGTT TCCACAGCAC CACCAGTAGA CGTGTTCCCT 
GTGGCGGTGG AAATGGCTGT TGATCCTCCT GCTCCTGCAG CAACACCGAC CCCCAGGACC 
CCGCAGCCTA GTGAGGATCC AGACGCCCCT CAGGGGGAGA GAGATGGAGA AGGCCAGTGG 
ACATCTTCCA CATCAGTAGA GCAGGCCAGG GAGCGGGGCT ACCACAGGGC CTTATTGCTG 
AGTGATCATC GCAAAGATAC AGATGGTGGA GAGTCAGAAG GTGGTGATTC CCAAGTGAAG 
GACTGTTCTT CTCCCAAGAG CTGCAAGAGC CCCTCGACAC ATGCACCTTG TTCTCCAGCA 
GCCCAGGCTG TGTCTCGGCC ATCAAAGGAT GAAGATGAAG CACAGACTCG TGGTCCTGCT 
CAGCCTCAGG CAGTGTCCGT CCAGCCGTCC AGCGCTAAGA CTCCCAGCTC AAAACCAGCT 
GCTCTGACGC CCAGCAAGCT CCACTGTGGG CCCTCAGGCG CCTCTCAGAG TCACTACGCG 
GGACCTTCGC TCATGCACTC TCCCAAAGCG CAACCCCAAG GATCACCTTT CCGTGGCCAG 
CGAGCCTTTC TGACAGCTCA GCCTCAGAAC CAGCCTCAAC CACAAGCTAC ATCTGGCCCT 
GCAACTTTCC CTCAGTATAA CCCTCAAAAT GCACCTCCAC CTCCGCCCCC TCCTCCCCCA 
GCTCCACCTA CATCTGCTCC TTACTTCCCC ACCCAGCCTA CTGCTGCTGC TGCTCCCTTT 
CCTGCATTTA AGCCCACCGT AGCCTCGCCC TTTCCTCCTG GTTCCCAGCC TCTACTTCAG 
ACCCATCATG CACTGCATTA CCAAAGCAGT GCTGCTCCTC CTCCTCCACC ACCTCCTCCC 
CCTCCCCACC CGCAGCCTGG CCCCACCTTA CTGCATGTCA ATCTGCAACC TCCTTCACTG 
CAGCAGCATC AGCTCCTGCT GAGCTCCGCC CCACCTCCGC CTCCTCCTCC ACCTCCTCAG 
ACACAGAGTT CCCAGCAGCC CCCGCCTAAC TCCGGCACGC TCCTGTCAAT CAAACAAGGA 
CCACACCCAC CCCTTCCACC CCCTCCTCCA GCTCCCTCCA CTAACGCACC ACCGCACCCG 
TTCCAAAACA TCAGTGGCTT TCAAACCACT CTACTCCACC AGTCTGCACC AGCCAACCCC 
TCAGTAACCG CATCTACTTA TCAACAAACT GTATTACCCC CTCCTCCTCC ACCTCCCCCC 
CAACAAACTC CTCCCACACA GACCCCGCCC AATCCGAGCA TCCCTCAGAT CGCAGGTGGC 
AATCGAGGAC CCGCTCCTTC CTCCGCCTCT TTCCACACCA CCGGATACTT GGGCACAGGA 
TGGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_005164910.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164967.1 Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X6, mRNA.

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CloneID	ODa09485
Clone ID Related Accession (Same CDS sequence)	XM_005164910.3 , XM_005164910.4
Accession Version	XM_005164910.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	histone-lysine N-methyltransferase 2E isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164910.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACGGCCAACA CCTCGTATCT GGACATGGCT 
GCAGGCTCAG AACCAGAATC TGTGGAGGCG AGCTCTGTGG TAGTTGAGAA GTCCACCTAC 
CCTCACCAGA TTTACAGCAG CAGCTCCCAC CACTCCCACG GATACATCGG TCTGCCTTAC 
GCCGACCATA ATTATGGGGC GCGACCCCCA CCCACCCCGC CGGCGTCCCC ACCTCCCTCG 
GTGCTGATCC GGCCGGGCGA GAGCTTGTTT GTGTCCGGAA GCCGGCCTGG TGAGAACTTG 
TTTGTGCCGG GCGGGCAGGA CGAGGCGTCG CGTGGCACCA CGCTCAGCAC CTCGGAGGAC 
GGCAGCTACG GTGCTGACAT CACACGCTGC ATCTGTGGCT TCACCCACGA CGATGGCTAC 
ATGATCTGCT GCGACAAGTG CAGTGTGTGG CAGCACATAG ACTGCATGGG GATCGACAGG 
CAGCACATTC CCGAAACCTA CCTGTGTGAG CGCTGTCAGC CACGCACCCT GGACAGAGAG 
CACGCCATCC TCCTGCAAAC CAGGAAGAGA GAGAACATGT CTGATGGGGA CACCAGTGCT 
ACAGAAAGCG GAGATGAGGT TCCACTAGAG CTGTACACAG CGTTTCAGCA CACCCCTACC 
AGCATCACAC TCACAACAGC ACGTCTGGGT AACAAACAGG CTGATAAGAA ACGAAAGAAG 
AGCGGAGAGA AGGAGCCGCC GGCTACAGCC AGGGCCAAGA AGTCATTCCG TGAAGGCTCT 
AGAAAATCAT CTAGAGTAAA GGGCTCCGCT CCCGAGTGTG AGCCGATTGA TCCTCCCTCT 
CTATGGGAGA ATAAGATGAA GTCGTGGATG GAGCGCTACG AGGAGGCCAG CAGTAACCAG 
TACAGTGAAG AAGTGCGGCT ACTGCTGAGA GTCAAAGAAG CCCGAGATGG AAAAACCCTG 
GCCTACAATA CACACACCGC AGCCTTCAAG CCACCTGTAG AGAGTCAAGT ACAAAAAAAC 
AAGCGAATTC TGAAGGCTGT GCGGGATTTG GCTGCTGATT CATTAATAAT CGAGTACAGG 
GGGAAGGTCA TGCTAAGACA GCAGTTTGAG GCTAATGGCT ACTTCTTTAA GAGGCCATAC 
CCTTTTGTAT TGTTCTACTC TAAGTTTGAC GGTTTGGAAA TGTGCGTAGA CGCACGGAGC 
TTTGGGAATG AGGCCCGATT CATTCGGCGC TCCTGCACCC CTAACGCTGA AGTGCGGCAC 
GTGATTGAAG AGGGCATGCT GCATTTATAC ATTTACTCTC TGAGGTCCAT CTCCAAAGGG 
AGCGAGATCA CCATTGGCTT TGATTACGAC TACGGCAGCT GTAAATATAA GGTGGACTGT 
GCTTGTGTAA AAGGCAATCC GGAGTGTCCT GTGCTCAAGC ATAACCTGGA GCCCACAGAG 
AATCTAGGCT CTAGCACACG ACGGCGAGGC CGTAAGGACA AAGAACCATC GCGGGACGAG 
AGCGGACAAA ATCAGAACCT CACTATGGAC TGTGACGGGT CCAAGGGGAA AACGCTTAAT 
GACGCCAAAC AGAGAAAACT CTCTCCTCTC AGGCTCTCAA TATCTAACAA TCAGACACGG 
GAAGAGAGGA AGATGGAGGC CATTCTGCAG GCGTTTGCTC GTATGGAGAA GAGGGAAAAG 
CGGAGGGAGC AAGCGTTGGA GAGGATTGGC ACCAAAGGGG AGGTTGGTGG ACGCAGCGAT 
ATAAAGGAGG AGGCACCTGC AACCCCTGAG GCTGAATCTC CTGCCGTGAT ACAGCCGCTG 
CTAGAGGTAG TAAAGGAGGA GCCTGGTCTG AAACCGGCGA AGGTGAGCCG TAACAAACAG 
AGAAAAAGCT TCTCCCGGAA CCGGACGCAC ATAGGACAGC AGAGGCGGCG AGCACGAACT 
GTCAGCACCT GCTCCGACTT GCCCCCCAGT TCCCCTGGTG ACACTTTAGA GCCCTTAATG 
GCAGAGACTC AAGATATAGA GATGCCTGTG GCCCCTGAAG CCGATGCTCC GCCAACGCAA 
CCCCCTGAAA CAAGCCCACC CCACAGTGGC TCACCAGCCC CAACATGCCG CAGTGGACAG 
AAGTACCCCA AAACTAAAAA GCACTTAGTG AGTGAGTGGA TGGGTGTTGA CAAACAGGAG 
CGGGTTGCGT CACGCACTCC GGAGCCTCCC CCTGAAAGGC CGTTGCGGAT CAGTAGTGAT 
CCTGAAGTCT TAGCCACACA ACTGAACTCT TTACCGGGCA TGTCCTGCAG CTCACACGTC 
TACAGCACGC CAAAACACTA CGTCCGCTTT TCTTCTCCTT TCCTGGCCAA CCGTAGTCCC 
AGTACTCCTG GAGTGCCCAC GGGACGACGG CGCTCGAGAG AGATGCCTGA AACGCCAACT 
TCCACTGGCT CCTGCAAGAA GCGCTGGCTA AAGCAGGCTT TAGAAGAAGA AGGTTCGACC 
AGCCCAGGTG GAGGACGACC TTTTCTGCTC ATACCCAGTG AGAGTCCTCT CAGTCCCTCT 
ATAAACGGCG AGTCCTGCAG CCCTCTACCC CTCAATGGAA GTTGCTCATT ACCAGAGTTG 
CCTACACCGT TGAAGAAGAG GCGCTTGTGT TCACTCGATC CCTGCATGTC GGAGACTTCC 
ACCCCATATG GGTCTCCATG TGCCACGCCA ACCCGAACAG AGTCAACAGA AGCACCGGGC 
ACACCTTCGC TGCTAGCAAC GCCTCCTCGA CCTCGACCAG AGGAGCCCAG TACCGAGCCC 
TCGACACCCT TGCAGATGCC CAACCACCCT CTGTTACAGG AGAGTGAATC GTCAATGGAC 
AGTTCTCCAG ATGGCAGCCG GAGGCCCAGT ACACAAGATG TTGAGCGACC TCCTTCGCTG 
CTGGCATCTC CCAGCGTGAG AAATGTGAGC TCTGATACGG CTCCACAGGA GTCTAGTAAA 
TCTATGGGGT CTCTGAGTCC TCAGCCCTCC CACACGGAGC CCCAGGATGC CGCAGTGGAT 
GAAGGCATGG AGGTTGATGC TGCTGCTGCT GTTACTTCTG CTGCTGGTGG TGGTGGTGGT 
TCAGAAGCAT CAACATCAGC TCCTGAAACG CCAGTCTCCT CTTACCCTCC ATGGATGAAG 
AGTCCAGAGC GGGGCGGCAT CTCTTTTTCC CCAGTGAATT CAAATCTGAG AGACCTCACT 
CCTTCACACA CCCTGGAGAT GGGGGCGTAC AGGCCGGATT CAACCTCCAG TGGCCCTTTT 
AGTGAAGCCG CACCCTTCTT TTCTTGCAAT GAGGAGAGCA GTGGTGTGAC CTTTACCCGC 
TCGCTAAGTG GAGATGGCAC AGGGGAGGGA AGCACTGCAA AAAACCCACA GAAGAAAAAG 
GTCTCTCTGT TGGAGTACAG AAAGCGTCAG CGTGAGGCAC GGCGGAGCGG CTCCAAAGGC 
GAGTGTAGCT CCCCTGTTTC CACAGCACCA CCAGTAGACG TGTTCCCTGT GGCGGTGGAA 
ATGGCTGTTG ATCCTCCTGC TCCTGCAGCA ACACCGACCC CCAGGACCCC GCAGCCTAGT 
GAGGATCCAG ACGCCCCTCA GGGGGAGAGA GATGGAGAAG GCCAGTGGAC ATCTTCCACA 
TCAGTAGAGC AGGCCAGGGA GCGGGGCTAC CACAGGGCCT TATTGCTGAG TGATCATCGC 
AAAGATACAG ATGGTGGAGA GTCAGAAGGT GGTGATTCCC AAGTGAAGGA CTGTTCTTCT 
CCCAAGAGCT GCAAGAGCCC CTCGACACAT GCACCTTGTT CTCCAGCAGC CCAGGCTGTG 
TCTCGGCCAT CAAAGGATGA AGATGAAGCA CAGACTCGTG GTCCTGCTCA GCCTCAGGCA 
GTGTCCGTCC AGCCGTCCAG CGCTAAGACT CCCAGCTCAA AACCAGCTGC TCTGACGCCC 
AGCAAGCTCC ACTGTGGGCC CTCAGGCGCC TCTCAGAGTC ACTACGCGGG ACCTTCGCTC 
ATGCACTCTC CCAAAGCGCA ACCCCAAGGA TCACCTTTCC GTGGCCAGCG AGCCTTTCTG 
ACAGCTCAGC CTCAGAACCA GCCTCAACCA CAAGCTACAT CTGGCCCTGC AACTTTCCCT 
CAGTATAACC CTCAAAATGC ACCTCCACCT CCGCCCCCTC CTCCCCCAGC TCCACCTACA 
TCTGCTCCTT ACTTCCCCAC CCAGCCTACT GCTGCTGCTG CTCCCTTTCC TGCATTTAAG 
CCCACCGTAG CCTCGCCCTT TCCTCCTGGT TCCCAGCCTC TACTTCAGAC CCATCATGCA 
CTGCATTACC AAAGCAGTGC TGCTCCTCCT CCTCCACCAC CTCCTCCCCC TCCCCACCCG 
CAGCCTGGCC CCACCTTACT GCATGTCAAT CTGCAACCTC CTTCACTGCA GCAGCATCAG 
CTCCTGCTGA GCTCCGCCCC ACCTCCGCCT CCTCCTCCAC CTCCTCAGAC ACAGAGTTCC 
CAGCAGCCCC CGCCTAACTC CGGCACGCTC CTGTCAATCA AACAAGGACC ACACCCACCC 
CTTCCACCCC CTCCTCCAGC TCCCTCCACT AACGCACCAC CGCACCCGTT CCAAAACATC 
AGTGGCTTTC AAACCACTCT ACTCCACCAG TCTGCACCAG CCAACCCCTC AGTAACCGCA 
TCTACTTATC AACAAACTGT ATTACCCCCT CCTCCTCCAC CTCCCCCCCA ACAAACTCCT 
CCCACACAGA CCCCGCCCAA TCCGAGCATC CCTCAGATCG CAGGTGGCAA TCGAGGACCC 
GCTCCTTCCT CCGCCTCTTT CCACACCACC GGATACTTGG GCACAGGATG GCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164967.1
CDS	1143..5939
Translation	MSIVIPVGVDTANTSYLDMAAGSEPESVEASSVVVEKSTYPHQIYSSSSHHSHGYIGLPYADHNYGARPPPTPPASPPPSVLIRPGESLFVSGSRPGENLFVPGGQDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQHIPETYLCERCQPRTLDREHAILLQTRKRENMSDGDTSATESGDEVPLELYTAFQHTPTSITLTTARLGNKQADKKRKKSGEKEPPATARAKKSFREGSRKSSRVKGSAPECEPIDPPSLWENKMKSWMERYEEASSNQYSEEVRLLLRVKEARDGKTLAYNTHTAAFKPPVESQVQKNKRILKAVRDLAADSLIIEYRGKVMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARSFGNEARFIRRSCTPNAEVRHVIEEGMLHLYIYSLRSISKGSEITIGFDYDYGSCKYKVDCACVKGNPECPVLKHNLEPTENLGSSTRRRGRKDKEPSRDESGQNQNLTMDCDGSKGKTLNDAKQRKLSPLRLSISNNQTREERKMEAILQAFARMEKREKRREQALERIGTKGEVGGRSDIKEEAPATPEAESPAVIQPLLEVVKEEPGLKPAKVSRNKQRKSFSRNRTHIGQQRRRARTVSTCSDLPPSSPGDTLEPLMAETQDIEMPVAPEADAPPTQPPETSPPHSGSPAPTCRSGQKYPKTKKHLVSEWMGVDKQERVASRTPEPPPERPLRISSDPEVLATQLNSLPGMSCSSHVYSTPKHYVRFSSPFLANRSPSTPGVPTGRRRSREMPETPTSTGSCKKRWLKQALEEEGSTSPGGGRPFLLIPSESPLSPSINGESCSPLPLNGSCSLPELPTPLKKRRLCSLDPCMSETSTPYGSPCATPTRTESTEAPGTPSLLATPPRPRPEEPSTEPSTPLQMPNHPLLQESESSMDSSPDGSRRPSTQDVERPPSLLASPSVRNVSSDTAPQESSKSMGSLSPQPSHTEPQDAAVDEGMEVDAAAAVTSAAGGGGGSEASTSAPETPVSSYPPWMKSPERGGISFSPVNSNLRDLTPSHTLEMGAYRPDSTSSGPFSEAAPFFSCNEESSGVTFTRSLSGDGTGEGSTAKNPQKKKVSLLEYRKRQREARRSGSKGECSSPVSTAPPVDVFPVAVEMAVDPPAPAATPTPRTPQPSEDPDAPQGERDGEGQWTSSTSVEQARERGYHRALLLSDHRKDTDGGESEGGDSQVKDCSSPKSCKSPSTHAPCSPAAQAVSRPSKDEDEAQTRGPAQPQAVSVQPSSAKTPSSKPAALTPSKLHCGPSGASQSHYAGPSLMHSPKAQPQGSPFRGQRAFLTAQPQNQPQPQATSGPATFPQYNPQNAPPPPPPPPPAPPTSAPYFPTQPTAAAAPFPAFKPTVASPFPPGSQPLLQTHHALHYQSSAAPPPPPPPPPPHPQPGPTLLHVNLQPPSLQQHQLLLSSAPPPPPPPPPQTQSSQQPPPNSGTLLSIKQGPHPPLPPPPPAPSTNAPPHPFQNISGFQTTLLHQSAPANPSVTASTYQQTVLPPPPPPPPQQTPPTQTPPNPSIPQIAGGNRGPAPSSASFHTTGYLGTGWH

Target ORF information:

RefSeq Version	XM_005164910.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164910.3

ORF Insert Sequence:

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACGGCCAACA CCTCGTATCT GGACATGGCT 
GCAGGCTCAG AACCAGAATC TGTGGAGGCG AGCTCTGTGG TAGTTGAGAA GTCCACCTAC 
CCTCACCAGA TTTACAGCAG CAGCTCCCAC CACTCCCACG GATACATCGG TCTGCCTTAC 
GCCGACCATA ATTATGGGGC GCGACCCCCA CCCACCCCGC CGGCGTCCCC ACCTCCCTCG 
GTGCTGATCC GGCCGGGCGA GAGCTTGTTT GTGTCCGGAA GCCGGCCTGG TGAGAACTTG 
TTTGTGCCGG GCGGGCAGGA CGAGGCGTCG CGTGGCACCA CGCTCAGCAC CTCGGAGGAC 
GGCAGCTACG GTGCTGACAT CACACGCTGC ATCTGTGGCT TCACCCACGA CGATGGCTAC 
ATGATCTGCT GCGACAAGTG CAGTGTGTGG CAGCACATAG ACTGCATGGG GATCGACAGG 
CAGCACATTC CCGAAACCTA CCTGTGTGAG CGCTGTCAGC CACGCACCCT GGACAGAGAG 
CACGCCATCC TCCTGCAAAC CAGGAAGAGA GAGAACATGT CTGATGGGGA CACCAGTGCT 
ACAGAAAGCG GAGATGAGGT TCCACTAGAG CTGTACACAG CGTTTCAGCA CACCCCTACC 
AGCATCACAC TCACAACAGC ACGTCTGGGT AACAAACAGG CTGATAAGAA ACGAAAGAAG 
AGCGGAGAGA AGGAGCCGCC GGCTACAGCC AGGGCCAAGA AGTCATTCCG TGAAGGCTCT 
AGAAAATCAT CTAGAGTAAA GGGCTCCGCT CCCGAGTGTG AGCCGATTGA TCCTCCCTCT 
CTATGGGAGA ATAAGATGAA GTCGTGGATG GAGCGCTACG AGGAGGCCAG CAGTAACCAG 
TACAGTGAAG AAGTGCGGCT ACTGCTGAGA GTCAAAGAAG CCCGAGATGG AAAAACCCTG 
GCCTACAATA CACACACCGC AGCCTTCAAG CCACCTGTAG AGAGTCAAGT ACAAAAAAAC 
AAGCGAATTC TGAAGGCTGT GCGGGATTTG GCTGCTGATT CATTAATAAT CGAGTACAGG 
GGGAAGGTCA TGCTAAGACA GCAGTTTGAG GCTAATGGCT ACTTCTTTAA GAGGCCATAC 
CCTTTTGTAT TGTTCTACTC TAAGTTTGAC GGTTTGGAAA TGTGCGTAGA CGCACGGAGC 
TTTGGGAATG AGGCCCGATT CATTCGGCGC TCCTGCACCC CTAACGCTGA AGTGCGGCAC 
GTGATTGAAG AGGGCATGCT GCATTTATAC ATTTACTCTC TGAGGTCCAT CTCCAAAGGG 
AGCGAGATCA CCATTGGCTT TGATTACGAC TACGGCAGCT GTAAATATAA GGTGGACTGT 
GCTTGTGTAA AAGGCAATCC GGAGTGTCCT GTGCTCAAGC ATAACCTGGA GCCCACAGAG 
AATCTAGGCT CTAGCACACG ACGGCGAGGC CGTAAGGACA AAGAACCATC GCGGGACGAG 
AGCGGACAAA ATCAGAACCT CACTATGGAC TGTGACGGGT CCAAGGGGAA AACGCTTAAT 
GACGCCAAAC AGAGAAAACT CTCTCCTCTC AGGCTCTCAA TATCTAACAA TCAGACACGG 
GAAGAGAGGA AGATGGAGGC CATTCTGCAG GCGTTTGCTC GTATGGAGAA GAGGGAAAAG 
CGGAGGGAGC AAGCGTTGGA GAGGATTGGC ACCAAAGGGG AGGTTGGTGG ACGCAGCGAT 
ATAAAGGAGG AGGCACCTGC AACCCCTGAG GCTGAATCTC CTGCCGTGAT ACAGCCGCTG 
CTAGAGGTAG TAAAGGAGGA GCCTGGTCTG AAACCGGCGA AGGTGAGCCG TAACAAACAG 
AGAAAAAGCT TCTCCCGGAA CCGGACGCAC ATAGGACAGC AGAGGCGGCG AGCACGAACT 
GTCAGCACCT GCTCCGACTT GCCCCCCAGT TCCCCTGGTG ACACTTTAGA GCCCTTAATG 
GCAGAGACTC AAGATATAGA GATGCCTGTG GCCCCTGAAG CCGATGCTCC GCCAACGCAA 
CCCCCTGAAA CAAGCCCACC CCACAGTGGC TCACCAGCCC CAACATGCCG CAGTGGACAG 
AAGTACCCCA AAACTAAAAA GCACTTAGTG AGTGAGTGGA TGGGTGTTGA CAAACAGGAG 
CGGGTTGCGT CACGCACTCC GGAGCCTCCC CCTGAAAGGC CGTTGCGGAT CAGTAGTGAT 
CCTGAAGTCT TAGCCACACA ACTGAACTCT TTACCGGGCA TGTCCTGCAG CTCACACGTC 
TACAGCACGC CAAAACACTA CGTCCGCTTT TCTTCTCCTT TCCTGGCCAA CCGTAGTCCC 
AGTACTCCTG GAGTGCCCAC GGGACGACGG CGCTCGAGAG AGATGCCTGA AACGCCAACT 
TCCACTGGCT CCTGCAAGAA GCGCTGGCTA AAGCAGGCTT TAGAAGAAGA AGGTTCGACC 
AGCCCAGGTG GAGGACGACC TTTTCTGCTC ATACCCAGTG AGAGTCCTCT CAGTCCCTCT 
ATAAACGGCG AGTCCTGCAG CCCTCTACCC CTCAATGGAA GTTGCTCATT ACCAGAGTTG 
CCTACACCGT TGAAGAAGAG GCGCTTGTGT TCACTCGATC CCTGCATGTC GGAGACTTCC 
ACCCCATATG GGTCTCCATG TGCCACGCCA ACCCGAACAG AGTCAACAGA AGCACCGGGC 
ACACCTTCGC TGCTAGCAAC GCCTCCTCGA CCTCGACCAG AGGAGCCCAG TACCGAGCCC 
TCGACACCCT TGCAGATGCC CAACCACCCT CTGTTACAGG AGAGTGAATC GTCAATGGAC 
AGTTCTCCAG ATGGCAGCCG GAGGCCCAGT ACACAAGATG TTGAGCGACC TCCTTCGCTG 
CTGGCATCTC CCAGCGTGAG AAATGTGAGC TCTGATACGG CTCCACAGGA GTCTAGTAAA 
TCTATGGGGT CTCTGAGTCC TCAGCCCTCC CACACGGAGC CCCAGGATGC CGCAGTGGAT 
GAAGGCATGG AGGTTGATGC TGCTGCTGCT GTTACTTCTG CTGCTGGTGG TGGTGGTGGT 
TCAGAAGCAT CAACATCAGC TCCTGAAACG CCAGTCTCCT CTTACCCTCC ATGGATGAAG 
AGTCCAGAGC GGGGCGGCAT CTCTTTTTCC CCAGTGAATT CAAATCTGAG AGACCTCACT 
CCTTCACACA CCCTGGAGAT GGGGGCGTAC AGGCCGGATT CAACCTCCAG TGGCCCTTTT 
AGTGAAGCCG CACCCTTCTT TTCTTGCAAT GAGGAGAGCA GTGGTGTGAC CTTTACCCGC 
TCGCTAAGTG GAGATGGCAC AGGGGAGGGA AGCACTGCAA AAAACCCACA GAAGAAAAAG 
GTCTCTCTGT TGGAGTACAG AAAGCGTCAG CGTGAGGCAC GGCGGAGCGG CTCCAAAGGC 
GAGTGTAGCT CCCCTGTTTC CACAGCACCA CCAGTAGACG TGTTCCCTGT GGCGGTGGAA 
ATGGCTGTTG ATCCTCCTGC TCCTGCAGCA ACACCGACCC CCAGGACCCC GCAGCCTAGT 
GAGGATCCAG ACGCCCCTCA GGGGGAGAGA GATGGAGAAG GCCAGTGGAC ATCTTCCACA 
TCAGTAGAGC AGGCCAGGGA GCGGGGCTAC CACAGGGCCT TATTGCTGAG TGATCATCGC 
AAAGATACAG ATGGTGGAGA GTCAGAAGGT GGTGATTCCC AAGTGAAGGA CTGTTCTTCT 
CCCAAGAGCT GCAAGAGCCC CTCGACACAT GCACCTTGTT CTCCAGCAGC CCAGGCTGTG 
TCTCGGCCAT CAAAGGATGA AGATGAAGCA CAGACTCGTG GTCCTGCTCA GCCTCAGGCA 
GTGTCCGTCC AGCCGTCCAG CGCTAAGACT CCCAGCTCAA AACCAGCTGC TCTGACGCCC 
AGCAAGCTCC ACTGTGGGCC CTCAGGCGCC TCTCAGAGTC ACTACGCGGG ACCTTCGCTC 
ATGCACTCTC CCAAAGCGCA ACCCCAAGGA TCACCTTTCC GTGGCCAGCG AGCCTTTCTG 
ACAGCTCAGC CTCAGAACCA GCCTCAACCA CAAGCTACAT CTGGCCCTGC AACTTTCCCT 
CAGTATAACC CTCAAAATGC ACCTCCACCT CCGCCCCCTC CTCCCCCAGC TCCACCTACA 
TCTGCTCCTT ACTTCCCCAC CCAGCCTACT GCTGCTGCTG CTCCCTTTCC TGCATTTAAG 
CCCACCGTAG CCTCGCCCTT TCCTCCTGGT TCCCAGCCTC TACTTCAGAC CCATCATGCA 
CTGCATTACC AAAGCAGTGC TGCTCCTCCT CCTCCACCAC CTCCTCCCCC TCCCCACCCG 
CAGCCTGGCC CCACCTTACT GCATGTCAAT CTGCAACCTC CTTCACTGCA GCAGCATCAG 
CTCCTGCTGA GCTCCGCCCC ACCTCCGCCT CCTCCTCCAC CTCCTCAGAC ACAGAGTTCC 
CAGCAGCCCC CGCCTAACTC CGGCACGCTC CTGTCAATCA AACAAGGACC ACACCCACCC 
CTTCCACCCC CTCCTCCAGC TCCCTCCACT AACGCACCAC CGCACCCGTT CCAAAACATC 
AGTGGCTTTC AAACCACTCT ACTCCACCAG TCTGCACCAG CCAACCCCTC AGTAACCGCA 
TCTACTTATC AACAAACTGT ATTACCCCCT CCTCCTCCAC CTCCCCCCCA ACAAACTCCT 
CCCACACAGA CCCCGCCCAA TCCGAGCATC CCTCAGATCG CAGGTGGCAA TCGAGGACCC 
GCTCCTTCCT CCGCCTCTTT CCACACCACC GGATACTTGG GCACAGGATG GCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_005164910.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164967.1 Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X5, mRNA.

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CloneID	ODa09485
Clone ID Related Accession (Same CDS sequence)	XM_005164910.3 , XM_005164910.4
Accession Version	XM_005164910.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	histone-lysine N-methyltransferase 2E isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164910.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACGGCCAACA CCTCGTATCT GGACATGGCT 
GCAGGCTCAG AACCAGAATC TGTGGAGGCG AGCTCTGTGG TAGTTGAGAA GTCCACCTAC 
CCTCACCAGA TTTACAGCAG CAGCTCCCAC CACTCCCACG GATACATCGG TCTGCCTTAC 
GCCGACCATA ATTATGGGGC GCGACCCCCA CCCACCCCGC CGGCGTCCCC ACCTCCCTCG 
GTGCTGATCC GGCCGGGCGA GAGCTTGTTT GTGTCCGGAA GCCGGCCTGG TGAGAACTTG 
TTTGTGCCGG GCGGGCAGGA CGAGGCGTCG CGTGGCACCA CGCTCAGCAC CTCGGAGGAC 
GGCAGCTACG GTGCTGACAT CACACGCTGC ATCTGTGGCT TCACCCACGA CGATGGCTAC 
ATGATCTGCT GCGACAAGTG CAGTGTGTGG CAGCACATAG ACTGCATGGG GATCGACAGG 
CAGCACATTC CCGAAACCTA CCTGTGTGAG CGCTGTCAGC CACGCACCCT GGACAGAGAG 
CACGCCATCC TCCTGCAAAC CAGGAAGAGA GAGAACATGT CTGATGGGGA CACCAGTGCT 
ACAGAAAGCG GAGATGAGGT TCCACTAGAG CTGTACACAG CGTTTCAGCA CACCCCTACC 
AGCATCACAC TCACAACAGC ACGTCTGGGT AACAAACAGG CTGATAAGAA ACGAAAGAAG 
AGCGGAGAGA AGGAGCCGCC GGCTACAGCC AGGGCCAAGA AGTCATTCCG TGAAGGCTCT 
AGAAAATCAT CTAGAGTAAA GGGCTCCGCT CCCGAGTGTG AGCCGATTGA TCCTCCCTCT 
CTATGGGAGA ATAAGATGAA GTCGTGGATG GAGCGCTACG AGGAGGCCAG CAGTAACCAG 
TACAGTGAAG AAGTGCGGCT ACTGCTGAGA GTCAAAGAAG CCCGAGATGG AAAAACCCTG 
GCCTACAATA CACACACCGC AGCCTTCAAG CCACCTGTAG AGAGTCAAGT ACAAAAAAAC 
AAGCGAATTC TGAAGGCTGT GCGGGATTTG GCTGCTGATT CATTAATAAT CGAGTACAGG 
GGGAAGGTCA TGCTAAGACA GCAGTTTGAG GCTAATGGCT ACTTCTTTAA GAGGCCATAC 
CCTTTTGTAT TGTTCTACTC TAAGTTTGAC GGTTTGGAAA TGTGCGTAGA CGCACGGAGC 
TTTGGGAATG AGGCCCGATT CATTCGGCGC TCCTGCACCC CTAACGCTGA AGTGCGGCAC 
GTGATTGAAG AGGGCATGCT GCATTTATAC ATTTACTCTC TGAGGTCCAT CTCCAAAGGG 
AGCGAGATCA CCATTGGCTT TGATTACGAC TACGGCAGCT GTAAATATAA GGTGGACTGT 
GCTTGTGTAA AAGGCAATCC GGAGTGTCCT GTGCTCAAGC ATAACCTGGA GCCCACAGAG 
AATCTAGGCT CTAGCACACG ACGGCGAGGC CGTAAGGACA AAGAACCATC GCGGGACGAG 
AGCGGACAAA ATCAGAACCT CACTATGGAC TGTGACGGGT CCAAGGGGAA AACGCTTAAT 
GACGCCAAAC AGAGAAAACT CTCTCCTCTC AGGCTCTCAA TATCTAACAA TCAGACACGG 
GAAGAGAGGA AGATGGAGGC CATTCTGCAG GCGTTTGCTC GTATGGAGAA GAGGGAAAAG 
CGGAGGGAGC AAGCGTTGGA GAGGATTGGC ACCAAAGGGG AGGTTGGTGG ACGCAGCGAT 
ATAAAGGAGG AGGCACCTGC AACCCCTGAG GCTGAATCTC CTGCCGTGAT ACAGCCGCTG 
CTAGAGGTAG TAAAGGAGGA GCCTGGTCTG AAACCGGCGA AGGTGAGCCG TAACAAACAG 
AGAAAAAGCT TCTCCCGGAA CCGGACGCAC ATAGGACAGC AGAGGCGGCG AGCACGAACT 
GTCAGCACCT GCTCCGACTT GCCCCCCAGT TCCCCTGGTG ACACTTTAGA GCCCTTAATG 
GCAGAGACTC AAGATATAGA GATGCCTGTG GCCCCTGAAG CCGATGCTCC GCCAACGCAA 
CCCCCTGAAA CAAGCCCACC CCACAGTGGC TCACCAGCCC CAACATGCCG CAGTGGACAG 
AAGTACCCCA AAACTAAAAA GCACTTAGTG AGTGAGTGGA TGGGTGTTGA CAAACAGGAG 
CGGGTTGCGT CACGCACTCC GGAGCCTCCC CCTGAAAGGC CGTTGCGGAT CAGTAGTGAT 
CCTGAAGTCT TAGCCACACA ACTGAACTCT TTACCGGGCA TGTCCTGCAG CTCACACGTC 
TACAGCACGC CAAAACACTA CGTCCGCTTT TCTTCTCCTT TCCTGGCCAA CCGTAGTCCC 
AGTACTCCTG GAGTGCCCAC GGGACGACGG CGCTCGAGAG AGATGCCTGA AACGCCAACT 
TCCACTGGCT CCTGCAAGAA GCGCTGGCTA AAGCAGGCTT TAGAAGAAGA AGGTTCGACC 
AGCCCAGGTG GAGGACGACC TTTTCTGCTC ATACCCAGTG AGAGTCCTCT CAGTCCCTCT 
ATAAACGGCG AGTCCTGCAG CCCTCTACCC CTCAATGGAA GTTGCTCATT ACCAGAGTTG 
CCTACACCGT TGAAGAAGAG GCGCTTGTGT TCACTCGATC CCTGCATGTC GGAGACTTCC 
ACCCCATATG GGTCTCCATG TGCCACGCCA ACCCGAACAG AGTCAACAGA AGCACCGGGC 
ACACCTTCGC TGCTAGCAAC GCCTCCTCGA CCTCGACCAG AGGAGCCCAG TACCGAGCCC 
TCGACACCCT TGCAGATGCC CAACCACCCT CTGTTACAGG AGAGTGAATC GTCAATGGAC 
AGTTCTCCAG ATGGCAGCCG GAGGCCCAGT ACACAAGATG TTGAGCGACC TCCTTCGCTG 
CTGGCATCTC CCAGCGTGAG AAATGTGAGC TCTGATACGG CTCCACAGGA GTCTAGTAAA 
TCTATGGGGT CTCTGAGTCC TCAGCCCTCC CACACGGAGC CCCAGGATGC CGCAGTGGAT 
GAAGGCATGG AGGTTGATGC TGCTGCTGCT GTTACTTCTG CTGCTGGTGG TGGTGGTGGT 
TCAGAAGCAT CAACATCAGC TCCTGAAACG CCAGTCTCCT CTTACCCTCC ATGGATGAAG 
AGTCCAGAGC GGGGCGGCAT CTCTTTTTCC CCAGTGAATT CAAATCTGAG AGACCTCACT 
CCTTCACACA CCCTGGAGAT GGGGGCGTAC AGGCCGGATT CAACCTCCAG TGGCCCTTTT 
AGTGAAGCCG CACCCTTCTT TTCTTGCAAT GAGGAGAGCA GTGGTGTGAC CTTTACCCGC 
TCGCTAAGTG GAGATGGCAC AGGGGAGGGA AGCACTGCAA AAAACCCACA GAAGAAAAAG 
GTCTCTCTGT TGGAGTACAG AAAGCGTCAG CGTGAGGCAC GGCGGAGCGG CTCCAAAGGC 
GAGTGTAGCT CCCCTGTTTC CACAGCACCA CCAGTAGACG TGTTCCCTGT GGCGGTGGAA 
ATGGCTGTTG ATCCTCCTGC TCCTGCAGCA ACACCGACCC CCAGGACCCC GCAGCCTAGT 
GAGGATCCAG ACGCCCCTCA GGGGGAGAGA GATGGAGAAG GCCAGTGGAC ATCTTCCACA 
TCAGTAGAGC AGGCCAGGGA GCGGGGCTAC CACAGGGCCT TATTGCTGAG TGATCATCGC 
AAAGATACAG ATGGTGGAGA GTCAGAAGGT GGTGATTCCC AAGTGAAGGA CTGTTCTTCT 
CCCAAGAGCT GCAAGAGCCC CTCGACACAT GCACCTTGTT CTCCAGCAGC CCAGGCTGTG 
TCTCGGCCAT CAAAGGATGA AGATGAAGCA CAGACTCGTG GTCCTGCTCA GCCTCAGGCA 
GTGTCCGTCC AGCCGTCCAG CGCTAAGACT CCCAGCTCAA AACCAGCTGC TCTGACGCCC 
AGCAAGCTCC ACTGTGGGCC CTCAGGCGCC TCTCAGAGTC ACTACGCGGG ACCTTCGCTC 
ATGCACTCTC CCAAAGCGCA ACCCCAAGGA TCACCTTTCC GTGGCCAGCG AGCCTTTCTG 
ACAGCTCAGC CTCAGAACCA GCCTCAACCA CAAGCTACAT CTGGCCCTGC AACTTTCCCT 
CAGTATAACC CTCAAAATGC ACCTCCACCT CCGCCCCCTC CTCCCCCAGC TCCACCTACA 
TCTGCTCCTT ACTTCCCCAC CCAGCCTACT GCTGCTGCTG CTCCCTTTCC TGCATTTAAG 
CCCACCGTAG CCTCGCCCTT TCCTCCTGGT TCCCAGCCTC TACTTCAGAC CCATCATGCA 
CTGCATTACC AAAGCAGTGC TGCTCCTCCT CCTCCACCAC CTCCTCCCCC TCCCCACCCG 
CAGCCTGGCC CCACCTTACT GCATGTCAAT CTGCAACCTC CTTCACTGCA GCAGCATCAG 
CTCCTGCTGA GCTCCGCCCC ACCTCCGCCT CCTCCTCCAC CTCCTCAGAC ACAGAGTTCC 
CAGCAGCCCC CGCCTAACTC CGGCACGCTC CTGTCAATCA AACAAGGACC ACACCCACCC 
CTTCCACCCC CTCCTCCAGC TCCCTCCACT AACGCACCAC CGCACCCGTT CCAAAACATC 
AGTGGCTTTC AAACCACTCT ACTCCACCAG TCTGCACCAG CCAACCCCTC AGTAACCGCA 
TCTACTTATC AACAAACTGT ATTACCCCCT CCTCCTCCAC CTCCCCCCCA ACAAACTCCT 
CCCACACAGA CCCCGCCCAA TCCGAGCATC CCTCAGATCG CAGGTGGCAA TCGAGGACCC 
GCTCCTTCCT CCGCCTCTTT CCACACCACC GGATACTTGG GCACAGGATG GCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164967.1
CDS	1079..5875
Translation	MSIVIPVGVDTANTSYLDMAAGSEPESVEASSVVVEKSTYPHQIYSSSSHHSHGYIGLPYADHNYGARPPPTPPASPPPSVLIRPGESLFVSGSRPGENLFVPGGQDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQHIPETYLCERCQPRTLDREHAILLQTRKRENMSDGDTSATESGDEVPLELYTAFQHTPTSITLTTARLGNKQADKKRKKSGEKEPPATARAKKSFREGSRKSSRVKGSAPECEPIDPPSLWENKMKSWMERYEEASSNQYSEEVRLLLRVKEARDGKTLAYNTHTAAFKPPVESQVQKNKRILKAVRDLAADSLIIEYRGKVMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARSFGNEARFIRRSCTPNAEVRHVIEEGMLHLYIYSLRSISKGSEITIGFDYDYGSCKYKVDCACVKGNPECPVLKHNLEPTENLGSSTRRRGRKDKEPSRDESGQNQNLTMDCDGSKGKTLNDAKQRKLSPLRLSISNNQTREERKMEAILQAFARMEKREKRREQALERIGTKGEVGGRSDIKEEAPATPEAESPAVIQPLLEVVKEEPGLKPAKVSRNKQRKSFSRNRTHIGQQRRRARTVSTCSDLPPSSPGDTLEPLMAETQDIEMPVAPEADAPPTQPPETSPPHSGSPAPTCRSGQKYPKTKKHLVSEWMGVDKQERVASRTPEPPPERPLRISSDPEVLATQLNSLPGMSCSSHVYSTPKHYVRFSSPFLANRSPSTPGVPTGRRRSREMPETPTSTGSCKKRWLKQALEEEGSTSPGGGRPFLLIPSESPLSPSINGESCSPLPLNGSCSLPELPTPLKKRRLCSLDPCMSETSTPYGSPCATPTRTESTEAPGTPSLLATPPRPRPEEPSTEPSTPLQMPNHPLLQESESSMDSSPDGSRRPSTQDVERPPSLLASPSVRNVSSDTAPQESSKSMGSLSPQPSHTEPQDAAVDEGMEVDAAAAVTSAAGGGGGSEASTSAPETPVSSYPPWMKSPERGGISFSPVNSNLRDLTPSHTLEMGAYRPDSTSSGPFSEAAPFFSCNEESSGVTFTRSLSGDGTGEGSTAKNPQKKKVSLLEYRKRQREARRSGSKGECSSPVSTAPPVDVFPVAVEMAVDPPAPAATPTPRTPQPSEDPDAPQGERDGEGQWTSSTSVEQARERGYHRALLLSDHRKDTDGGESEGGDSQVKDCSSPKSCKSPSTHAPCSPAAQAVSRPSKDEDEAQTRGPAQPQAVSVQPSSAKTPSSKPAALTPSKLHCGPSGASQSHYAGPSLMHSPKAQPQGSPFRGQRAFLTAQPQNQPQPQATSGPATFPQYNPQNAPPPPPPPPPAPPTSAPYFPTQPTAAAAPFPAFKPTVASPFPPGSQPLLQTHHALHYQSSAAPPPPPPPPPPHPQPGPTLLHVNLQPPSLQQHQLLLSSAPPPPPPPPPQTQSSQQPPPNSGTLLSIKQGPHPPLPPPPPAPSTNAPPHPFQNISGFQTTLLHQSAPANPSVTASTYQQTVLPPPPPPPPQQTPPTQTPPNPSIPQIAGGNRGPAPSSASFHTTGYLGTGWH

Target ORF information:

RefSeq Version	XM_005164910.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164910.4

ORF Insert Sequence:

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACGGCCAACA CCTCGTATCT GGACATGGCT 
GCAGGCTCAG AACCAGAATC TGTGGAGGCG AGCTCTGTGG TAGTTGAGAA GTCCACCTAC 
CCTCACCAGA TTTACAGCAG CAGCTCCCAC CACTCCCACG GATACATCGG TCTGCCTTAC 
GCCGACCATA ATTATGGGGC GCGACCCCCA CCCACCCCGC CGGCGTCCCC ACCTCCCTCG 
GTGCTGATCC GGCCGGGCGA GAGCTTGTTT GTGTCCGGAA GCCGGCCTGG TGAGAACTTG 
TTTGTGCCGG GCGGGCAGGA CGAGGCGTCG CGTGGCACCA CGCTCAGCAC CTCGGAGGAC 
GGCAGCTACG GTGCTGACAT CACACGCTGC ATCTGTGGCT TCACCCACGA CGATGGCTAC 
ATGATCTGCT GCGACAAGTG CAGTGTGTGG CAGCACATAG ACTGCATGGG GATCGACAGG 
CAGCACATTC CCGAAACCTA CCTGTGTGAG CGCTGTCAGC CACGCACCCT GGACAGAGAG 
CACGCCATCC TCCTGCAAAC CAGGAAGAGA GAGAACATGT CTGATGGGGA CACCAGTGCT 
ACAGAAAGCG GAGATGAGGT TCCACTAGAG CTGTACACAG CGTTTCAGCA CACCCCTACC 
AGCATCACAC TCACAACAGC ACGTCTGGGT AACAAACAGG CTGATAAGAA ACGAAAGAAG 
AGCGGAGAGA AGGAGCCGCC GGCTACAGCC AGGGCCAAGA AGTCATTCCG TGAAGGCTCT 
AGAAAATCAT CTAGAGTAAA GGGCTCCGCT CCCGAGTGTG AGCCGATTGA TCCTCCCTCT 
CTATGGGAGA ATAAGATGAA GTCGTGGATG GAGCGCTACG AGGAGGCCAG CAGTAACCAG 
TACAGTGAAG AAGTGCGGCT ACTGCTGAGA GTCAAAGAAG CCCGAGATGG AAAAACCCTG 
GCCTACAATA CACACACCGC AGCCTTCAAG CCACCTGTAG AGAGTCAAGT ACAAAAAAAC 
AAGCGAATTC TGAAGGCTGT GCGGGATTTG GCTGCTGATT CATTAATAAT CGAGTACAGG 
GGGAAGGTCA TGCTAAGACA GCAGTTTGAG GCTAATGGCT ACTTCTTTAA GAGGCCATAC 
CCTTTTGTAT TGTTCTACTC TAAGTTTGAC GGTTTGGAAA TGTGCGTAGA CGCACGGAGC 
TTTGGGAATG AGGCCCGATT CATTCGGCGC TCCTGCACCC CTAACGCTGA AGTGCGGCAC 
GTGATTGAAG AGGGCATGCT GCATTTATAC ATTTACTCTC TGAGGTCCAT CTCCAAAGGG 
AGCGAGATCA CCATTGGCTT TGATTACGAC TACGGCAGCT GTAAATATAA GGTGGACTGT 
GCTTGTGTAA AAGGCAATCC GGAGTGTCCT GTGCTCAAGC ATAACCTGGA GCCCACAGAG 
AATCTAGGCT CTAGCACACG ACGGCGAGGC CGTAAGGACA AAGAACCATC GCGGGACGAG 
AGCGGACAAA ATCAGAACCT CACTATGGAC TGTGACGGGT CCAAGGGGAA AACGCTTAAT 
GACGCCAAAC AGAGAAAACT CTCTCCTCTC AGGCTCTCAA TATCTAACAA TCAGACACGG 
GAAGAGAGGA AGATGGAGGC CATTCTGCAG GCGTTTGCTC GTATGGAGAA GAGGGAAAAG 
CGGAGGGAGC AAGCGTTGGA GAGGATTGGC ACCAAAGGGG AGGTTGGTGG ACGCAGCGAT 
ATAAAGGAGG AGGCACCTGC AACCCCTGAG GCTGAATCTC CTGCCGTGAT ACAGCCGCTG 
CTAGAGGTAG TAAAGGAGGA GCCTGGTCTG AAACCGGCGA AGGTGAGCCG TAACAAACAG 
AGAAAAAGCT TCTCCCGGAA CCGGACGCAC ATAGGACAGC AGAGGCGGCG AGCACGAACT 
GTCAGCACCT GCTCCGACTT GCCCCCCAGT TCCCCTGGTG ACACTTTAGA GCCCTTAATG 
GCAGAGACTC AAGATATAGA GATGCCTGTG GCCCCTGAAG CCGATGCTCC GCCAACGCAA 
CCCCCTGAAA CAAGCCCACC CCACAGTGGC TCACCAGCCC CAACATGCCG CAGTGGACAG 
AAGTACCCCA AAACTAAAAA GCACTTAGTG AGTGAGTGGA TGGGTGTTGA CAAACAGGAG 
CGGGTTGCGT CACGCACTCC GGAGCCTCCC CCTGAAAGGC CGTTGCGGAT CAGTAGTGAT 
CCTGAAGTCT TAGCCACACA ACTGAACTCT TTACCGGGCA TGTCCTGCAG CTCACACGTC 
TACAGCACGC CAAAACACTA CGTCCGCTTT TCTTCTCCTT TCCTGGCCAA CCGTAGTCCC 
AGTACTCCTG GAGTGCCCAC GGGACGACGG CGCTCGAGAG AGATGCCTGA AACGCCAACT 
TCCACTGGCT CCTGCAAGAA GCGCTGGCTA AAGCAGGCTT TAGAAGAAGA AGGTTCGACC 
AGCCCAGGTG GAGGACGACC TTTTCTGCTC ATACCCAGTG AGAGTCCTCT CAGTCCCTCT 
ATAAACGGCG AGTCCTGCAG CCCTCTACCC CTCAATGGAA GTTGCTCATT ACCAGAGTTG 
CCTACACCGT TGAAGAAGAG GCGCTTGTGT TCACTCGATC CCTGCATGTC GGAGACTTCC 
ACCCCATATG GGTCTCCATG TGCCACGCCA ACCCGAACAG AGTCAACAGA AGCACCGGGC 
ACACCTTCGC TGCTAGCAAC GCCTCCTCGA CCTCGACCAG AGGAGCCCAG TACCGAGCCC 
TCGACACCCT TGCAGATGCC CAACCACCCT CTGTTACAGG AGAGTGAATC GTCAATGGAC 
AGTTCTCCAG ATGGCAGCCG GAGGCCCAGT ACACAAGATG TTGAGCGACC TCCTTCGCTG 
CTGGCATCTC CCAGCGTGAG AAATGTGAGC TCTGATACGG CTCCACAGGA GTCTAGTAAA 
TCTATGGGGT CTCTGAGTCC TCAGCCCTCC CACACGGAGC CCCAGGATGC CGCAGTGGAT 
GAAGGCATGG AGGTTGATGC TGCTGCTGCT GTTACTTCTG CTGCTGGTGG TGGTGGTGGT 
TCAGAAGCAT CAACATCAGC TCCTGAAACG CCAGTCTCCT CTTACCCTCC ATGGATGAAG 
AGTCCAGAGC GGGGCGGCAT CTCTTTTTCC CCAGTGAATT CAAATCTGAG AGACCTCACT 
CCTTCACACA CCCTGGAGAT GGGGGCGTAC AGGCCGGATT CAACCTCCAG TGGCCCTTTT 
AGTGAAGCCG CACCCTTCTT TTCTTGCAAT GAGGAGAGCA GTGGTGTGAC CTTTACCCGC 
TCGCTAAGTG GAGATGGCAC AGGGGAGGGA AGCACTGCAA AAAACCCACA GAAGAAAAAG 
GTCTCTCTGT TGGAGTACAG AAAGCGTCAG CGTGAGGCAC GGCGGAGCGG CTCCAAAGGC 
GAGTGTAGCT CCCCTGTTTC CACAGCACCA CCAGTAGACG TGTTCCCTGT GGCGGTGGAA 
ATGGCTGTTG ATCCTCCTGC TCCTGCAGCA ACACCGACCC CCAGGACCCC GCAGCCTAGT 
GAGGATCCAG ACGCCCCTCA GGGGGAGAGA GATGGAGAAG GCCAGTGGAC ATCTTCCACA 
TCAGTAGAGC AGGCCAGGGA GCGGGGCTAC CACAGGGCCT TATTGCTGAG TGATCATCGC 
AAAGATACAG ATGGTGGAGA GTCAGAAGGT GGTGATTCCC AAGTGAAGGA CTGTTCTTCT 
CCCAAGAGCT GCAAGAGCCC CTCGACACAT GCACCTTGTT CTCCAGCAGC CCAGGCTGTG 
TCTCGGCCAT CAAAGGATGA AGATGAAGCA CAGACTCGTG GTCCTGCTCA GCCTCAGGCA 
GTGTCCGTCC AGCCGTCCAG CGCTAAGACT CCCAGCTCAA AACCAGCTGC TCTGACGCCC 
AGCAAGCTCC ACTGTGGGCC CTCAGGCGCC TCTCAGAGTC ACTACGCGGG ACCTTCGCTC 
ATGCACTCTC CCAAAGCGCA ACCCCAAGGA TCACCTTTCC GTGGCCAGCG AGCCTTTCTG 
ACAGCTCAGC CTCAGAACCA GCCTCAACCA CAAGCTACAT CTGGCCCTGC AACTTTCCCT 
CAGTATAACC CTCAAAATGC ACCTCCACCT CCGCCCCCTC CTCCCCCAGC TCCACCTACA 
TCTGCTCCTT ACTTCCCCAC CCAGCCTACT GCTGCTGCTG CTCCCTTTCC TGCATTTAAG 
CCCACCGTAG CCTCGCCCTT TCCTCCTGGT TCCCAGCCTC TACTTCAGAC CCATCATGCA 
CTGCATTACC AAAGCAGTGC TGCTCCTCCT CCTCCACCAC CTCCTCCCCC TCCCCACCCG 
CAGCCTGGCC CCACCTTACT GCATGTCAAT CTGCAACCTC CTTCACTGCA GCAGCATCAG 
CTCCTGCTGA GCTCCGCCCC ACCTCCGCCT CCTCCTCCAC CTCCTCAGAC ACAGAGTTCC 
CAGCAGCCCC CGCCTAACTC CGGCACGCTC CTGTCAATCA AACAAGGACC ACACCCACCC 
CTTCCACCCC CTCCTCCAGC TCCCTCCACT AACGCACCAC CGCACCCGTT CCAAAACATC 
AGTGGCTTTC AAACCACTCT ACTCCACCAG TCTGCACCAG CCAACCCCTC AGTAACCGCA 
TCTACTTATC AACAAACTGT ATTACCCCCT CCTCCTCCAC CTCCCCCCCA ACAAACTCCT 
CCCACACAGA CCCCGCCCAA TCCGAGCATC CCTCAGATCG CAGGTGGCAA TCGAGGACCC 
GCTCCTTCCT CCGCCTCTTT CCACACCACC GGATACTTGG GCACAGGATG GCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_009300440.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298715.1 Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X2, mRNA.

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CloneID	ODa39844
Clone ID Related Accession (Same CDS sequence)	XM_009300440.2 , XM_009300439.2 , XM_009300441.2 , XM_009300439.3 , XM_021475266.1
Accession Version	XM_009300440.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	histone-lysine N-methyltransferase 2E isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300440.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACGGCCAACA CCTCGTATCT GGACATGGCT 
GCAGGCTCAG AACCAGAATC TGTGGAGGCG AGCTCTGTGG TAGTTGAGAA GTCCACCTAC 
CCTCACCAGA TTTACAGCAG CAGCTCCCAC CACTCCCACG GATACATCGG TCTGCCTTAC 
GCCGACCATA ATTATGGGGC GCGACCCCCA CCCACCCCGC CGGCGTCCCC ACCTCCCTCG 
GTGCTGATCC GGCCGGGCGA GAGCTTGTTT GTGTCCGGAA GCCGGCCTGG TGAGAACTTG 
TTTGTGCCGG GCGGGCAGGA CGAGGCGTCG CGTGGCACCA CGCTCAGCAC CTCGGAGGAC 
GGCAGCTACG GTGCTGACAT CACACGCTGC ATCTGTGGCT TCACCCACGA CGATGGCTAC 
ATGATCTGCT GCGACAAGTG CAGTGTGTGG CAGCACATAG ACTGCATGGG GATCGACAGG 
CAGCACATTC CCGAAACCTA CCTGTGTGAG CGCTGTCAGC CACGCACCCT GGACAGAGAG 
CACGCCATCC TCCTGCAAAC CAGGAAGAGA GAGAACATGT CTGATGGGGA CACCAGTGCT 
ACAGAAAGCG GAGATGAGGT TCCACTAGAG CTGTACACAG CGTTTCAGCA CACCCCTACC 
AGCATCACAC TCACAACAGC ACGTCTGGGT AACAAACAGG CTGATAAGAA ACGAAAGAAG 
AGCGGAGAGA AGGAGCCGCC GGCTACAGCC AGGGCCAAGA AGTCATTCCG TGAAGGCTCT 
AGAAAATCAT CTAGAGTAAA GGGCTCCGCT CCCGAGTGTG AGCCGATTGA TCCTCCCTCT 
CTATGGGAGA ATAAGATGAA GTCGTGGATG GAGCGCTACG AGGAGGCCAG CAGTAACCAG 
TACAGTGAAG AAGTGCGGCT ACTGCTGAGA GTCAAAGAAG CCCGAGATGG AAAAACCCTG 
GCCTACAATA CACACACCGC AGCCTTCAAG CCACCTGTAG AGAGTCAAGT ACAAAAAAAC 
AAGCGAATTC TGAAGGCTGT GCGGGATTTG GCTGCTGATT CATTAATAAT CGAGTACAGG 
GGGAAGGTCA TGCTAAGACA GCAGTTTGAG GCTAATGGCT ACTTCTTTAA GAGGCCATAC 
CCTTTTGTAT TGTTCTACTC TAAGTTTGAC GGTTTGGAAA TGTGCGTAGA CGCACGGAGC 
TTTGGGAATG AGGCCCGATT CATTCGGCGC TCCTGCACCC CTAACGCTGA AGTGCGGCAC 
GTGATTGAAG AGGGCATGCT GCATTTATAC ATTTACTCTC TGAGGTCCAT CTCCAAAGGG 
AGCGAGATCA CCATTGGCTT TGATTACGAC TACGGCAGCT GTAAATATAA GGTGGACTGT 
GCTTGTGTAA AAGGCAATCC GGAGTGTCCT GTGCTCAAGC ATAACCTGGA GCCCACAGAG 
AATCTAGGCT CTAGCACACG ACGGCGAGGC CGTAAGGACA AAGAACCATC GCGGGACGAG 
AGCGGACAAA ATCAGAACCT CACTATGGAC TGTGACGGGT CCAAGGGGAA AACGCTTAAT 
GACGCCAAAC AGAGAAAACT CTCTCCTCTC AGGCTCTCAA TATCTAACAA TCAGGATCCT 
GAGTTAATAG AGGATCTAGA AGAGAAAACC TCCGTTAGCA ATGAAGTAGA GATGGAATCA 
GAGGAGCAGA TTGCAGAAAG GAGGAGGAAG ATGGGCAGCC CGGCAGAGGA GTCCCACCGT 
CACGACGCAG GGGCTTCCTG CTGCAGAGCT CTGAGAAACA AGGAGACACG GGAAGAGAGG 
AAGATGGAGG CCATTCTGCA GGCGTTTGCT CGTATGGAGA AGAGGGAAAA GCGGAGGGAG 
CAAGCGTTGG AGAGGATTGG CACCAAAGGG GAGGTTGGTG GACGCAGCGA TATAAAGGAG 
GAGGCACCTG CAACCCCTGA GGCTGAATCT CCTGCCGTGA TACAGCCGCT GCTAGAGGTA 
GTAAAGGAGG AGCCTGGTCT GAAACCGGCG AAGGTGAGCC GTAACAAACA GAGAAAAAGC 
TTCTCCCGGA ACCGGACGCA CATAGGACAG CAGAGGCGGC GAGCACGAAC TGTCAGCACC 
TGCTCCGACT TGCCCCCCAG TTCCCCTGGT GACACTTTAG AGCCCTTAAT GGCAGAGACT 
CAAGATATAG AGATGCCTGT GGCCCCTGAA GCCGATGCTC CGCCAACGCA ACCCCCTGAA 
ACAAGCCCAC CCCACAGTGG CTCACCAGCC CCAACATGCC GCAGTGGACA GAAGTACCCC 
AAAACTAAAA AGCACTTAGT GAGTGAGTGG ATGGGTGTTG ACAAACAGGA GCGGGTTGCG 
TCACGCACTC CGGAGCCTCC CCCTGAAAGG CCGTTGCGGA TCAGTAGTGA TCCTGAAGTC 
TTAGCCACAC AACTGAACTC TTTACCGGGC ATGTCCTGCA GCTCACACGT CTACAGCACG 
CCAAAACACT ACGTCCGCTT TTCTTCTCCT TTCCTGGCCA ACCGTAGTCC CAGTACTCCT 
GGAGTGCCCA CGGGACGACG GCGCTCGAGA GAGATGCCTG AAACGCCAAC TTCCACTGGC 
TCCTGCAAGA AGCGCTGGCT AAAGCAGGCT TTAGAAGAAG AAGGTTCGAC CAGCCCAGGT 
GGAGGACGAC CTTTTCTGCT CATACCCAGT GAGAGTCCTC TCAGTCCCTC TATAAACGGC 
GAGTCCTGCA GCCCTCTACC CCTCAATGGA AGTTGCTCAT TACCAGAGTT GCCTACACCG 
TTGAAGAAGA GGCGCTTGTG TTCACTCGAT CCCTGCATGT CGGAGACTTC CACCCCATAT 
GGGTCTCCAT GTGCCACGCC AACCCGAACA GAGTCAACAG AAGCACCGGG CACACCTTCG 
CTGCTAGCAA CGCCTCCTCG ACCTCGACCA GAGGAGCCCA GTACCGAGCC CTCGACACCC 
TTGCAGATGC CCAACCACCC TCTGTTACAG GAGAGTGAAT CGTCAATGGA CAGTTCTCCA 
GATGGCAGCC GGAGGCCCAG TACACAAGAT GTTGAGCGAC CTCCTTCGCT GCTGGCATCT 
CCCAGCGTGA GAAATGTGAG CTCTGATACG GCTCCACAGG AGTCTAGTAA ATCTATGGGG 
TCTCTGAGTC CTCAGCCCTC CCACACGGAG CCCCAGGATG CCGCAGTGGA TGAAGGCATG 
GAGGTTGATG CTGCTGCTGC TGTTACTTCT GCTGCTGGTG GTGGTGGTGG TTCAGAAGCA 
TCAACATCAG CTCCTGAAAC GCCAGTCTCC TCTTACCCTC CATGGATGAA GAGTCCAGAG 
CGGGGCGGCA TCTCTTTTTC CCCAGTGAAT TCAAATCTGA GAGACCTCAC TCCTTCACAC 
ACCCTGGAGA TGGGGGCGTA CAGGCCGGAT TCAACCTCCA GTGGCCCTTT TAGTGAAGCC 
GCACCCTTCT TTTCTTGCAA TGAGGAGAGC AGTGGTGTGA CCTTTACCCG CTCGCTAAGT 
GGAGATGGCA CAGGGGAGGG AAGCACTGCA AAAAACCCAC AGAAGAAAAA GGTCTCTCTG 
TTGGAGTACA GAAAGCGTCA GCGTGAGGCA CGGCGGAGCG GCTCCAAAGG CGAGTGTAGC 
TCCCCTGTTT CCACAGCACC ACCAGTAGAC GTGTTCCCTG TGGCGGTGGA AATGGCTGTT 
GATCCTCCTG CTCCTGCAGC AACACCGACC CCCAGGACCC CGCAGCCTAG TGAGGATCCA 
GACGCCCCTC AGGGGGAGAG AGATGGAGAA GGCCAGTGGA CATCTTCCAC ATCAGTAGAG 
CAGGCCAGGG AGCGGGGCTA CCACAGGGCC TTATTGCTGA GTGATCATCG CAAAGATACA 
GATGGTGGAG AGTCAGAAGG TGGTGATTCC CAAGTGAAGG ACTGTTCTTC TCCCAAGAGC 
TGCAAGAGCC CCTCGACACA TGCACCTTGT TCTCCAGCAG CCCAGGCTGT GTCTCGGCCA 
TCAAAGGATG AAGATGAAGC ACAGACTCGT GGTCCTGCTC AGCCTCAGGC AGTGTCCGTC 
CAGCCGTCCA GCGCTAAGAC TCCCAGCTCA AAACCAGCTG CTCTGACGCC CAGCAAGCTC 
CACTGTGGGC CCTCAGGCGC CTCTCAGAGT CACTACGCGG GACCTTCGCT CATGCACTCT 
CCCAAAGCGC AACCCCAAGG ATCACCTTTC CGTGGCCAGC GAGCCTTTCT GACAGCTCAG 
CCTCAGAACC AGCCTCAACC ACAAGCTACA TCTGGCCCTG CAACTTTCCC TCAGTATAAC 
CCTCAAAATG CACCTCCACC TCCGCCCCCT CCTCCCCCAG CTCCACCTAC ATCTGCTCCT 
TACTTCCCCA CCCAGCCTAC TGCTGCTGCT GCTCCCTTTC CTGCATTTAA GCCCACCGTA 
GCCTCGCCCT TTCCTCCTGG TTCCCAGCCT CTACTTCAGA CCCATCATGC ACTGCATTAC 
CAAAGCAGTG CTGCTCCTCC TCCTCCACCA CCTCCTCCCC CTCCCCACCC GCAGCCTGGC 
CCCACCTTAC TGCATGTCAA TCTGCAACCT CCTTCACTGC AGCAGCATCA GCTCCTGCTG 
AGCTCCGCCC CACCTCCGCC TCCTCCTCCA CCTCCTCAGA CACAGAGTTC CCAGCAGCCC 
CCGCCTAACT CCGGCACGCT CCTGTCAATC AAACAAGGAC CACACCCACC CCTTCCACCC 
CCTCCTCCAG CTCCCTCCAC TAACGCACCA CCGCACCCGT TCCAAAACAT CAGTGGCTTT 
CAAACCACTC TACTCCACCA GTCTGCACCA GCCAACCCCT CAGTAACCGC ATCTACTTAT 
CAACAAACTG TATTACCCCC TCCTCCTCCA CCTCCCCCCC AACAAACTCC TCCCACACAG 
ACCCCGCCCA ATCCGAGCAT CCCTCAGATC GCAGGTGGCA ATCGAGGACC CGCTCCTTCC 
TCCGCCTCTT TCCACACCAC CGGATACTTG GGCACAGGAT GGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298715.1
CDS	211..5178
Translation	MSIVIPVGVDTANTSYLDMAAGSEPESVEASSVVVEKSTYPHQIYSSSSHHSHGYIGLPYADHNYGARPPPTPPASPPPSVLIRPGESLFVSGSRPGENLFVPGGQDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQHIPETYLCERCQPRTLDREHAILLQTRKRENMSDGDTSATESGDEVPLELYTAFQHTPTSITLTTARLGNKQADKKRKKSGEKEPPATARAKKSFREGSRKSSRVKGSAPECEPIDPPSLWENKMKSWMERYEEASSNQYSEEVRLLLRVKEARDGKTLAYNTHTAAFKPPVESQVQKNKRILKAVRDLAADSLIIEYRGKVMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARSFGNEARFIRRSCTPNAEVRHVIEEGMLHLYIYSLRSISKGSEITIGFDYDYGSCKYKVDCACVKGNPECPVLKHNLEPTENLGSSTRRRGRKDKEPSRDESGQNQNLTMDCDGSKGKTLNDAKQRKLSPLRLSISNNQDPELIEDLEEKTSVSNEVEMESEEQIAERRRKMGSPAEESHRHDAGASCCRALRNKETREERKMEAILQAFARMEKREKRREQALERIGTKGEVGGRSDIKEEAPATPEAESPAVIQPLLEVVKEEPGLKPAKVSRNKQRKSFSRNRTHIGQQRRRARTVSTCSDLPPSSPGDTLEPLMAETQDIEMPVAPEADAPPTQPPETSPPHSGSPAPTCRSGQKYPKTKKHLVSEWMGVDKQERVASRTPEPPPERPLRISSDPEVLATQLNSLPGMSCSSHVYSTPKHYVRFSSPFLANRSPSTPGVPTGRRRSREMPETPTSTGSCKKRWLKQALEEEGSTSPGGGRPFLLIPSESPLSPSINGESCSPLPLNGSCSLPELPTPLKKRRLCSLDPCMSETSTPYGSPCATPTRTESTEAPGTPSLLATPPRPRPEEPSTEPSTPLQMPNHPLLQESESSMDSSPDGSRRPSTQDVERPPSLLASPSVRNVSSDTAPQESSKSMGSLSPQPSHTEPQDAAVDEGMEVDAAAAVTSAAGGGGGSEASTSAPETPVSSYPPWMKSPERGGISFSPVNSNLRDLTPSHTLEMGAYRPDSTSSGPFSEAAPFFSCNEESSGVTFTRSLSGDGTGEGSTAKNPQKKKVSLLEYRKRQREARRSGSKGECSSPVSTAPPVDVFPVAVEMAVDPPAPAATPTPRTPQPSEDPDAPQGERDGEGQWTSSTSVEQARERGYHRALLLSDHRKDTDGGESEGGDSQVKDCSSPKSCKSPSTHAPCSPAAQAVSRPSKDEDEAQTRGPAQPQAVSVQPSSAKTPSSKPAALTPSKLHCGPSGASQSHYAGPSLMHSPKAQPQGSPFRGQRAFLTAQPQNQPQPQATSGPATFPQYNPQNAPPPPPPPPPAPPTSAPYFPTQPTAAAAPFPAFKPTVASPFPPGSQPLLQTHHALHYQSSAAPPPPPPPPPPHPQPGPTLLHVNLQPPSLQQHQLLLSSAPPPPPPPPPQTQSSQQPPPNSGTLLSIKQGPHPPLPPPPPAPSTNAPPHPFQNISGFQTTLLHQSAPANPSVTASTYQQTVLPPPPPPPPQQTPPTQTPPNPSIPQIAGGNRGPAPSSASFHTTGYLGTGWH

Target ORF information:

RefSeq Version	XM_009300440.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300440.2

ORF Insert Sequence:

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACGGCCAACA CCTCGTATCT GGACATGGCT 
GCAGGCTCAG AACCAGAATC TGTGGAGGCG AGCTCTGTGG TAGTTGAGAA GTCCACCTAC 
CCTCACCAGA TTTACAGCAG CAGCTCCCAC CACTCCCACG GATACATCGG TCTGCCTTAC 
GCCGACCATA ATTATGGGGC GCGACCCCCA CCCACCCCGC CGGCGTCCCC ACCTCCCTCG 
GTGCTGATCC GGCCGGGCGA GAGCTTGTTT GTGTCCGGAA GCCGGCCTGG TGAGAACTTG 
TTTGTGCCGG GCGGGCAGGA CGAGGCGTCG CGTGGCACCA CGCTCAGCAC CTCGGAGGAC 
GGCAGCTACG GTGCTGACAT CACACGCTGC ATCTGTGGCT TCACCCACGA CGATGGCTAC 
ATGATCTGCT GCGACAAGTG CAGTGTGTGG CAGCACATAG ACTGCATGGG GATCGACAGG 
CAGCACATTC CCGAAACCTA CCTGTGTGAG CGCTGTCAGC CACGCACCCT GGACAGAGAG 
CACGCCATCC TCCTGCAAAC CAGGAAGAGA GAGAACATGT CTGATGGGGA CACCAGTGCT 
ACAGAAAGCG GAGATGAGGT TCCACTAGAG CTGTACACAG CGTTTCAGCA CACCCCTACC 
AGCATCACAC TCACAACAGC ACGTCTGGGT AACAAACAGG CTGATAAGAA ACGAAAGAAG 
AGCGGAGAGA AGGAGCCGCC GGCTACAGCC AGGGCCAAGA AGTCATTCCG TGAAGGCTCT 
AGAAAATCAT CTAGAGTAAA GGGCTCCGCT CCCGAGTGTG AGCCGATTGA TCCTCCCTCT 
CTATGGGAGA ATAAGATGAA GTCGTGGATG GAGCGCTACG AGGAGGCCAG CAGTAACCAG 
TACAGTGAAG AAGTGCGGCT ACTGCTGAGA GTCAAAGAAG CCCGAGATGG AAAAACCCTG 
GCCTACAATA CACACACCGC AGCCTTCAAG CCACCTGTAG AGAGTCAAGT ACAAAAAAAC 
AAGCGAATTC TGAAGGCTGT GCGGGATTTG GCTGCTGATT CATTAATAAT CGAGTACAGG 
GGGAAGGTCA TGCTAAGACA GCAGTTTGAG GCTAATGGCT ACTTCTTTAA GAGGCCATAC 
CCTTTTGTAT TGTTCTACTC TAAGTTTGAC GGTTTGGAAA TGTGCGTAGA CGCACGGAGC 
TTTGGGAATG AGGCCCGATT CATTCGGCGC TCCTGCACCC CTAACGCTGA AGTGCGGCAC 
GTGATTGAAG AGGGCATGCT GCATTTATAC ATTTACTCTC TGAGGTCCAT CTCCAAAGGG 
AGCGAGATCA CCATTGGCTT TGATTACGAC TACGGCAGCT GTAAATATAA GGTGGACTGT 
GCTTGTGTAA AAGGCAATCC GGAGTGTCCT GTGCTCAAGC ATAACCTGGA GCCCACAGAG 
AATCTAGGCT CTAGCACACG ACGGCGAGGC CGTAAGGACA AAGAACCATC GCGGGACGAG 
AGCGGACAAA ATCAGAACCT CACTATGGAC TGTGACGGGT CCAAGGGGAA AACGCTTAAT 
GACGCCAAAC AGAGAAAACT CTCTCCTCTC AGGCTCTCAA TATCTAACAA TCAGGATCCT 
GAGTTAATAG AGGATCTAGA AGAGAAAACC TCCGTTAGCA ATGAAGTAGA GATGGAATCA 
GAGGAGCAGA TTGCAGAAAG GAGGAGGAAG ATGGGCAGCC CGGCAGAGGA GTCCCACCGT 
CACGACGCAG GGGCTTCCTG CTGCAGAGCT CTGAGAAACA AGGAGACACG GGAAGAGAGG 
AAGATGGAGG CCATTCTGCA GGCGTTTGCT CGTATGGAGA AGAGGGAAAA GCGGAGGGAG 
CAAGCGTTGG AGAGGATTGG CACCAAAGGG GAGGTTGGTG GACGCAGCGA TATAAAGGAG 
GAGGCACCTG CAACCCCTGA GGCTGAATCT CCTGCCGTGA TACAGCCGCT GCTAGAGGTA 
GTAAAGGAGG AGCCTGGTCT GAAACCGGCG AAGGTGAGCC GTAACAAACA GAGAAAAAGC 
TTCTCCCGGA ACCGGACGCA CATAGGACAG CAGAGGCGGC GAGCACGAAC TGTCAGCACC 
TGCTCCGACT TGCCCCCCAG TTCCCCTGGT GACACTTTAG AGCCCTTAAT GGCAGAGACT 
CAAGATATAG AGATGCCTGT GGCCCCTGAA GCCGATGCTC CGCCAACGCA ACCCCCTGAA 
ACAAGCCCAC CCCACAGTGG CTCACCAGCC CCAACATGCC GCAGTGGACA GAAGTACCCC 
AAAACTAAAA AGCACTTAGT GAGTGAGTGG ATGGGTGTTG ACAAACAGGA GCGGGTTGCG 
TCACGCACTC CGGAGCCTCC CCCTGAAAGG CCGTTGCGGA TCAGTAGTGA TCCTGAAGTC 
TTAGCCACAC AACTGAACTC TTTACCGGGC ATGTCCTGCA GCTCACACGT CTACAGCACG 
CCAAAACACT ACGTCCGCTT TTCTTCTCCT TTCCTGGCCA ACCGTAGTCC CAGTACTCCT 
GGAGTGCCCA CGGGACGACG GCGCTCGAGA GAGATGCCTG AAACGCCAAC TTCCACTGGC 
TCCTGCAAGA AGCGCTGGCT AAAGCAGGCT TTAGAAGAAG AAGGTTCGAC CAGCCCAGGT 
GGAGGACGAC CTTTTCTGCT CATACCCAGT GAGAGTCCTC TCAGTCCCTC TATAAACGGC 
GAGTCCTGCA GCCCTCTACC CCTCAATGGA AGTTGCTCAT TACCAGAGTT GCCTACACCG 
TTGAAGAAGA GGCGCTTGTG TTCACTCGAT CCCTGCATGT CGGAGACTTC CACCCCATAT 
GGGTCTCCAT GTGCCACGCC AACCCGAACA GAGTCAACAG AAGCACCGGG CACACCTTCG 
CTGCTAGCAA CGCCTCCTCG ACCTCGACCA GAGGAGCCCA GTACCGAGCC CTCGACACCC 
TTGCAGATGC CCAACCACCC TCTGTTACAG GAGAGTGAAT CGTCAATGGA CAGTTCTCCA 
GATGGCAGCC GGAGGCCCAG TACACAAGAT GTTGAGCGAC CTCCTTCGCT GCTGGCATCT 
CCCAGCGTGA GAAATGTGAG CTCTGATACG GCTCCACAGG AGTCTAGTAA ATCTATGGGG 
TCTCTGAGTC CTCAGCCCTC CCACACGGAG CCCCAGGATG CCGCAGTGGA TGAAGGCATG 
GAGGTTGATG CTGCTGCTGC TGTTACTTCT GCTGCTGGTG GTGGTGGTGG TTCAGAAGCA 
TCAACATCAG CTCCTGAAAC GCCAGTCTCC TCTTACCCTC CATGGATGAA GAGTCCAGAG 
CGGGGCGGCA TCTCTTTTTC CCCAGTGAAT TCAAATCTGA GAGACCTCAC TCCTTCACAC 
ACCCTGGAGA TGGGGGCGTA CAGGCCGGAT TCAACCTCCA GTGGCCCTTT TAGTGAAGCC 
GCACCCTTCT TTTCTTGCAA TGAGGAGAGC AGTGGTGTGA CCTTTACCCG CTCGCTAAGT 
GGAGATGGCA CAGGGGAGGG AAGCACTGCA AAAAACCCAC AGAAGAAAAA GGTCTCTCTG 
TTGGAGTACA GAAAGCGTCA GCGTGAGGCA CGGCGGAGCG GCTCCAAAGG CGAGTGTAGC 
TCCCCTGTTT CCACAGCACC ACCAGTAGAC GTGTTCCCTG TGGCGGTGGA AATGGCTGTT 
GATCCTCCTG CTCCTGCAGC AACACCGACC CCCAGGACCC CGCAGCCTAG TGAGGATCCA 
GACGCCCCTC AGGGGGAGAG AGATGGAGAA GGCCAGTGGA CATCTTCCAC ATCAGTAGAG 
CAGGCCAGGG AGCGGGGCTA CCACAGGGCC TTATTGCTGA GTGATCATCG CAAAGATACA 
GATGGTGGAG AGTCAGAAGG TGGTGATTCC CAAGTGAAGG ACTGTTCTTC TCCCAAGAGC 
TGCAAGAGCC CCTCGACACA TGCACCTTGT TCTCCAGCAG CCCAGGCTGT GTCTCGGCCA 
TCAAAGGATG AAGATGAAGC ACAGACTCGT GGTCCTGCTC AGCCTCAGGC AGTGTCCGTC 
CAGCCGTCCA GCGCTAAGAC TCCCAGCTCA AAACCAGCTG CTCTGACGCC CAGCAAGCTC 
CACTGTGGGC CCTCAGGCGC CTCTCAGAGT CACTACGCGG GACCTTCGCT CATGCACTCT 
CCCAAAGCGC AACCCCAAGG ATCACCTTTC CGTGGCCAGC GAGCCTTTCT GACAGCTCAG 
CCTCAGAACC AGCCTCAACC ACAAGCTACA TCTGGCCCTG CAACTTTCCC TCAGTATAAC 
CCTCAAAATG CACCTCCACC TCCGCCCCCT CCTCCCCCAG CTCCACCTAC ATCTGCTCCT 
TACTTCCCCA CCCAGCCTAC TGCTGCTGCT GCTCCCTTTC CTGCATTTAA GCCCACCGTA 
GCCTCGCCCT TTCCTCCTGG TTCCCAGCCT CTACTTCAGA CCCATCATGC ACTGCATTAC 
CAAAGCAGTG CTGCTCCTCC TCCTCCACCA CCTCCTCCCC CTCCCCACCC GCAGCCTGGC 
CCCACCTTAC TGCATGTCAA TCTGCAACCT CCTTCACTGC AGCAGCATCA GCTCCTGCTG 
AGCTCCGCCC CACCTCCGCC TCCTCCTCCA CCTCCTCAGA CACAGAGTTC CCAGCAGCCC 
CCGCCTAACT CCGGCACGCT CCTGTCAATC AAACAAGGAC CACACCCACC CCTTCCACCC 
CCTCCTCCAG CTCCCTCCAC TAACGCACCA CCGCACCCGT TCCAAAACAT CAGTGGCTTT 
CAAACCACTC TACTCCACCA GTCTGCACCA GCCAACCCCT CAGTAACCGC ATCTACTTAT 
CAACAAACTG TATTACCCCC TCCTCCTCCA CCTCCCCCCC AACAAACTCC TCCCACACAG 
ACCCCGCCCA ATCCGAGCAT CCCTCAGATC GCAGGTGGCA ATCGAGGACC CGCTCCTTCC 
TCCGCCTCTT TCCACACCAC CGGATACTTG GGCACAGGAT GGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_009300439.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298714.1 Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa39844
Clone ID Related Accession (Same CDS sequence)	XM_009300440.2 , XM_009300439.2 , XM_009300441.2 , XM_009300439.3 , XM_021475266.1
Accession Version	XM_009300439.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	histone-lysine N-methyltransferase 2E isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300439.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACGGCCAACA CCTCGTATCT GGACATGGCT 
GCAGGCTCAG AACCAGAATC TGTGGAGGCG AGCTCTGTGG TAGTTGAGAA GTCCACCTAC 
CCTCACCAGA TTTACAGCAG CAGCTCCCAC CACTCCCACG GATACATCGG TCTGCCTTAC 
GCCGACCATA ATTATGGGGC GCGACCCCCA CCCACCCCGC CGGCGTCCCC ACCTCCCTCG 
GTGCTGATCC GGCCGGGCGA GAGCTTGTTT GTGTCCGGAA GCCGGCCTGG TGAGAACTTG 
TTTGTGCCGG GCGGGCAGGA CGAGGCGTCG CGTGGCACCA CGCTCAGCAC CTCGGAGGAC 
GGCAGCTACG GTGCTGACAT CACACGCTGC ATCTGTGGCT TCACCCACGA CGATGGCTAC 
ATGATCTGCT GCGACAAGTG CAGTGTGTGG CAGCACATAG ACTGCATGGG GATCGACAGG 
CAGCACATTC CCGAAACCTA CCTGTGTGAG CGCTGTCAGC CACGCACCCT GGACAGAGAG 
CACGCCATCC TCCTGCAAAC CAGGAAGAGA GAGAACATGT CTGATGGGGA CACCAGTGCT 
ACAGAAAGCG GAGATGAGGT TCCACTAGAG CTGTACACAG CGTTTCAGCA CACCCCTACC 
AGCATCACAC TCACAACAGC ACGTCTGGGT AACAAACAGG CTGATAAGAA ACGAAAGAAG 
AGCGGAGAGA AGGAGCCGCC GGCTACAGCC AGGGCCAAGA AGTCATTCCG TGAAGGCTCT 
AGAAAATCAT CTAGAGTAAA GGGCTCCGCT CCCGAGTGTG AGCCGATTGA TCCTCCCTCT 
CTATGGGAGA ATAAGATGAA GTCGTGGATG GAGCGCTACG AGGAGGCCAG CAGTAACCAG 
TACAGTGAAG AAGTGCGGCT ACTGCTGAGA GTCAAAGAAG CCCGAGATGG AAAAACCCTG 
GCCTACAATA CACACACCGC AGCCTTCAAG CCACCTGTAG AGAGTCAAGT ACAAAAAAAC 
AAGCGAATTC TGAAGGCTGT GCGGGATTTG GCTGCTGATT CATTAATAAT CGAGTACAGG 
GGGAAGGTCA TGCTAAGACA GCAGTTTGAG GCTAATGGCT ACTTCTTTAA GAGGCCATAC 
CCTTTTGTAT TGTTCTACTC TAAGTTTGAC GGTTTGGAAA TGTGCGTAGA CGCACGGAGC 
TTTGGGAATG AGGCCCGATT CATTCGGCGC TCCTGCACCC CTAACGCTGA AGTGCGGCAC 
GTGATTGAAG AGGGCATGCT GCATTTATAC ATTTACTCTC TGAGGTCCAT CTCCAAAGGG 
AGCGAGATCA CCATTGGCTT TGATTACGAC TACGGCAGCT GTAAATATAA GGTGGACTGT 
GCTTGTGTAA AAGGCAATCC GGAGTGTCCT GTGCTCAAGC ATAACCTGGA GCCCACAGAG 
AATCTAGGCT CTAGCACACG ACGGCGAGGC CGTAAGGACA AAGAACCATC GCGGGACGAG 
AGCGGACAAA ATCAGAACCT CACTATGGAC TGTGACGGGT CCAAGGGGAA AACGCTTAAT 
GACGCCAAAC AGAGAAAACT CTCTCCTCTC AGGCTCTCAA TATCTAACAA TCAGGATCCT 
GAGTTAATAG AGGATCTAGA AGAGAAAACC TCCGTTAGCA ATGAAGTAGA GATGGAATCA 
GAGGAGCAGA TTGCAGAAAG GAGGAGGAAG ATGGGCAGCC CGGCAGAGGA GTCCCACCGT 
CACGACGCAG GGGCTTCCTG CTGCAGAGCT CTGAGAAACA AGGAGACACG GGAAGAGAGG 
AAGATGGAGG CCATTCTGCA GGCGTTTGCT CGTATGGAGA AGAGGGAAAA GCGGAGGGAG 
CAAGCGTTGG AGAGGATTGG CACCAAAGGG GAGGTTGGTG GACGCAGCGA TATAAAGGAG 
GAGGCACCTG CAACCCCTGA GGCTGAATCT CCTGCCGTGA TACAGCCGCT GCTAGAGGTA 
GTAAAGGAGG AGCCTGGTCT GAAACCGGCG AAGGTGAGCC GTAACAAACA GAGAAAAAGC 
TTCTCCCGGA ACCGGACGCA CATAGGACAG CAGAGGCGGC GAGCACGAAC TGTCAGCACC 
TGCTCCGACT TGCCCCCCAG TTCCCCTGGT GACACTTTAG AGCCCTTAAT GGCAGAGACT 
CAAGATATAG AGATGCCTGT GGCCCCTGAA GCCGATGCTC CGCCAACGCA ACCCCCTGAA 
ACAAGCCCAC CCCACAGTGG CTCACCAGCC CCAACATGCC GCAGTGGACA GAAGTACCCC 
AAAACTAAAA AGCACTTAGT GAGTGAGTGG ATGGGTGTTG ACAAACAGGA GCGGGTTGCG 
TCACGCACTC CGGAGCCTCC CCCTGAAAGG CCGTTGCGGA TCAGTAGTGA TCCTGAAGTC 
TTAGCCACAC AACTGAACTC TTTACCGGGC ATGTCCTGCA GCTCACACGT CTACAGCACG 
CCAAAACACT ACGTCCGCTT TTCTTCTCCT TTCCTGGCCA ACCGTAGTCC CAGTACTCCT 
GGAGTGCCCA CGGGACGACG GCGCTCGAGA GAGATGCCTG AAACGCCAAC TTCCACTGGC 
TCCTGCAAGA AGCGCTGGCT AAAGCAGGCT TTAGAAGAAG AAGGTTCGAC CAGCCCAGGT 
GGAGGACGAC CTTTTCTGCT CATACCCAGT GAGAGTCCTC TCAGTCCCTC TATAAACGGC 
GAGTCCTGCA GCCCTCTACC CCTCAATGGA AGTTGCTCAT TACCAGAGTT GCCTACACCG 
TTGAAGAAGA GGCGCTTGTG TTCACTCGAT CCCTGCATGT CGGAGACTTC CACCCCATAT 
GGGTCTCCAT GTGCCACGCC AACCCGAACA GAGTCAACAG AAGCACCGGG CACACCTTCG 
CTGCTAGCAA CGCCTCCTCG ACCTCGACCA GAGGAGCCCA GTACCGAGCC CTCGACACCC 
TTGCAGATGC CCAACCACCC TCTGTTACAG GAGAGTGAAT CGTCAATGGA CAGTTCTCCA 
GATGGCAGCC GGAGGCCCAG TACACAAGAT GTTGAGCGAC CTCCTTCGCT GCTGGCATCT 
CCCAGCGTGA GAAATGTGAG CTCTGATACG GCTCCACAGG AGTCTAGTAA ATCTATGGGG 
TCTCTGAGTC CTCAGCCCTC CCACACGGAG CCCCAGGATG CCGCAGTGGA TGAAGGCATG 
GAGGTTGATG CTGCTGCTGC TGTTACTTCT GCTGCTGGTG GTGGTGGTGG TTCAGAAGCA 
TCAACATCAG CTCCTGAAAC GCCAGTCTCC TCTTACCCTC CATGGATGAA GAGTCCAGAG 
CGGGGCGGCA TCTCTTTTTC CCCAGTGAAT TCAAATCTGA GAGACCTCAC TCCTTCACAC 
ACCCTGGAGA TGGGGGCGTA CAGGCCGGAT TCAACCTCCA GTGGCCCTTT TAGTGAAGCC 
GCACCCTTCT TTTCTTGCAA TGAGGAGAGC AGTGGTGTGA CCTTTACCCG CTCGCTAAGT 
GGAGATGGCA CAGGGGAGGG AAGCACTGCA AAAAACCCAC AGAAGAAAAA GGTCTCTCTG 
TTGGAGTACA GAAAGCGTCA GCGTGAGGCA CGGCGGAGCG GCTCCAAAGG CGAGTGTAGC 
TCCCCTGTTT CCACAGCACC ACCAGTAGAC GTGTTCCCTG TGGCGGTGGA AATGGCTGTT 
GATCCTCCTG CTCCTGCAGC AACACCGACC CCCAGGACCC CGCAGCCTAG TGAGGATCCA 
GACGCCCCTC AGGGGGAGAG AGATGGAGAA GGCCAGTGGA CATCTTCCAC ATCAGTAGAG 
CAGGCCAGGG AGCGGGGCTA CCACAGGGCC TTATTGCTGA GTGATCATCG CAAAGATACA 
GATGGTGGAG AGTCAGAAGG TGGTGATTCC CAAGTGAAGG ACTGTTCTTC TCCCAAGAGC 
TGCAAGAGCC CCTCGACACA TGCACCTTGT TCTCCAGCAG CCCAGGCTGT GTCTCGGCCA 
TCAAAGGATG AAGATGAAGC ACAGACTCGT GGTCCTGCTC AGCCTCAGGC AGTGTCCGTC 
CAGCCGTCCA GCGCTAAGAC TCCCAGCTCA AAACCAGCTG CTCTGACGCC CAGCAAGCTC 
CACTGTGGGC CCTCAGGCGC CTCTCAGAGT CACTACGCGG GACCTTCGCT CATGCACTCT 
CCCAAAGCGC AACCCCAAGG ATCACCTTTC CGTGGCCAGC GAGCCTTTCT GACAGCTCAG 
CCTCAGAACC AGCCTCAACC ACAAGCTACA TCTGGCCCTG CAACTTTCCC TCAGTATAAC 
CCTCAAAATG CACCTCCACC TCCGCCCCCT CCTCCCCCAG CTCCACCTAC ATCTGCTCCT 
TACTTCCCCA CCCAGCCTAC TGCTGCTGCT GCTCCCTTTC CTGCATTTAA GCCCACCGTA 
GCCTCGCCCT TTCCTCCTGG TTCCCAGCCT CTACTTCAGA CCCATCATGC ACTGCATTAC 
CAAAGCAGTG CTGCTCCTCC TCCTCCACCA CCTCCTCCCC CTCCCCACCC GCAGCCTGGC 
CCCACCTTAC TGCATGTCAA TCTGCAACCT CCTTCACTGC AGCAGCATCA GCTCCTGCTG 
AGCTCCGCCC CACCTCCGCC TCCTCCTCCA CCTCCTCAGA CACAGAGTTC CCAGCAGCCC 
CCGCCTAACT CCGGCACGCT CCTGTCAATC AAACAAGGAC CACACCCACC CCTTCCACCC 
CCTCCTCCAG CTCCCTCCAC TAACGCACCA CCGCACCCGT TCCAAAACAT CAGTGGCTTT 
CAAACCACTC TACTCCACCA GTCTGCACCA GCCAACCCCT CAGTAACCGC ATCTACTTAT 
CAACAAACTG TATTACCCCC TCCTCCTCCA CCTCCCCCCC AACAAACTCC TCCCACACAG 
ACCCCGCCCA ATCCGAGCAT CCCTCAGATC GCAGGTGGCA ATCGAGGACC CGCTCCTTCC 
TCCGCCTCTT TCCACACCAC CGGATACTTG GGCACAGGAT GGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298714.1
CDS	1144..6111
Translation	MSIVIPVGVDTANTSYLDMAAGSEPESVEASSVVVEKSTYPHQIYSSSSHHSHGYIGLPYADHNYGARPPPTPPASPPPSVLIRPGESLFVSGSRPGENLFVPGGQDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQHIPETYLCERCQPRTLDREHAILLQTRKRENMSDGDTSATESGDEVPLELYTAFQHTPTSITLTTARLGNKQADKKRKKSGEKEPPATARAKKSFREGSRKSSRVKGSAPECEPIDPPSLWENKMKSWMERYEEASSNQYSEEVRLLLRVKEARDGKTLAYNTHTAAFKPPVESQVQKNKRILKAVRDLAADSLIIEYRGKVMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARSFGNEARFIRRSCTPNAEVRHVIEEGMLHLYIYSLRSISKGSEITIGFDYDYGSCKYKVDCACVKGNPECPVLKHNLEPTENLGSSTRRRGRKDKEPSRDESGQNQNLTMDCDGSKGKTLNDAKQRKLSPLRLSISNNQDPELIEDLEEKTSVSNEVEMESEEQIAERRRKMGSPAEESHRHDAGASCCRALRNKETREERKMEAILQAFARMEKREKRREQALERIGTKGEVGGRSDIKEEAPATPEAESPAVIQPLLEVVKEEPGLKPAKVSRNKQRKSFSRNRTHIGQQRRRARTVSTCSDLPPSSPGDTLEPLMAETQDIEMPVAPEADAPPTQPPETSPPHSGSPAPTCRSGQKYPKTKKHLVSEWMGVDKQERVASRTPEPPPERPLRISSDPEVLATQLNSLPGMSCSSHVYSTPKHYVRFSSPFLANRSPSTPGVPTGRRRSREMPETPTSTGSCKKRWLKQALEEEGSTSPGGGRPFLLIPSESPLSPSINGESCSPLPLNGSCSLPELPTPLKKRRLCSLDPCMSETSTPYGSPCATPTRTESTEAPGTPSLLATPPRPRPEEPSTEPSTPLQMPNHPLLQESESSMDSSPDGSRRPSTQDVERPPSLLASPSVRNVSSDTAPQESSKSMGSLSPQPSHTEPQDAAVDEGMEVDAAAAVTSAAGGGGGSEASTSAPETPVSSYPPWMKSPERGGISFSPVNSNLRDLTPSHTLEMGAYRPDSTSSGPFSEAAPFFSCNEESSGVTFTRSLSGDGTGEGSTAKNPQKKKVSLLEYRKRQREARRSGSKGECSSPVSTAPPVDVFPVAVEMAVDPPAPAATPTPRTPQPSEDPDAPQGERDGEGQWTSSTSVEQARERGYHRALLLSDHRKDTDGGESEGGDSQVKDCSSPKSCKSPSTHAPCSPAAQAVSRPSKDEDEAQTRGPAQPQAVSVQPSSAKTPSSKPAALTPSKLHCGPSGASQSHYAGPSLMHSPKAQPQGSPFRGQRAFLTAQPQNQPQPQATSGPATFPQYNPQNAPPPPPPPPPAPPTSAPYFPTQPTAAAAPFPAFKPTVASPFPPGSQPLLQTHHALHYQSSAAPPPPPPPPPPHPQPGPTLLHVNLQPPSLQQHQLLLSSAPPPPPPPPPQTQSSQQPPPNSGTLLSIKQGPHPPLPPPPPAPSTNAPPHPFQNISGFQTTLLHQSAPANPSVTASTYQQTVLPPPPPPPPQQTPPTQTPPNPSIPQIAGGNRGPAPSSASFHTTGYLGTGWH

Target ORF information:

RefSeq Version	XM_009300439.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300439.2

ORF Insert Sequence:

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACGGCCAACA CCTCGTATCT GGACATGGCT 
GCAGGCTCAG AACCAGAATC TGTGGAGGCG AGCTCTGTGG TAGTTGAGAA GTCCACCTAC 
CCTCACCAGA TTTACAGCAG CAGCTCCCAC CACTCCCACG GATACATCGG TCTGCCTTAC 
GCCGACCATA ATTATGGGGC GCGACCCCCA CCCACCCCGC CGGCGTCCCC ACCTCCCTCG 
GTGCTGATCC GGCCGGGCGA GAGCTTGTTT GTGTCCGGAA GCCGGCCTGG TGAGAACTTG 
TTTGTGCCGG GCGGGCAGGA CGAGGCGTCG CGTGGCACCA CGCTCAGCAC CTCGGAGGAC 
GGCAGCTACG GTGCTGACAT CACACGCTGC ATCTGTGGCT TCACCCACGA CGATGGCTAC 
ATGATCTGCT GCGACAAGTG CAGTGTGTGG CAGCACATAG ACTGCATGGG GATCGACAGG 
CAGCACATTC CCGAAACCTA CCTGTGTGAG CGCTGTCAGC CACGCACCCT GGACAGAGAG 
CACGCCATCC TCCTGCAAAC CAGGAAGAGA GAGAACATGT CTGATGGGGA CACCAGTGCT 
ACAGAAAGCG GAGATGAGGT TCCACTAGAG CTGTACACAG CGTTTCAGCA CACCCCTACC 
AGCATCACAC TCACAACAGC ACGTCTGGGT AACAAACAGG CTGATAAGAA ACGAAAGAAG 
AGCGGAGAGA AGGAGCCGCC GGCTACAGCC AGGGCCAAGA AGTCATTCCG TGAAGGCTCT 
AGAAAATCAT CTAGAGTAAA GGGCTCCGCT CCCGAGTGTG AGCCGATTGA TCCTCCCTCT 
CTATGGGAGA ATAAGATGAA GTCGTGGATG GAGCGCTACG AGGAGGCCAG CAGTAACCAG 
TACAGTGAAG AAGTGCGGCT ACTGCTGAGA GTCAAAGAAG CCCGAGATGG AAAAACCCTG 
GCCTACAATA CACACACCGC AGCCTTCAAG CCACCTGTAG AGAGTCAAGT ACAAAAAAAC 
AAGCGAATTC TGAAGGCTGT GCGGGATTTG GCTGCTGATT CATTAATAAT CGAGTACAGG 
GGGAAGGTCA TGCTAAGACA GCAGTTTGAG GCTAATGGCT ACTTCTTTAA GAGGCCATAC 
CCTTTTGTAT TGTTCTACTC TAAGTTTGAC GGTTTGGAAA TGTGCGTAGA CGCACGGAGC 
TTTGGGAATG AGGCCCGATT CATTCGGCGC TCCTGCACCC CTAACGCTGA AGTGCGGCAC 
GTGATTGAAG AGGGCATGCT GCATTTATAC ATTTACTCTC TGAGGTCCAT CTCCAAAGGG 
AGCGAGATCA CCATTGGCTT TGATTACGAC TACGGCAGCT GTAAATATAA GGTGGACTGT 
GCTTGTGTAA AAGGCAATCC GGAGTGTCCT GTGCTCAAGC ATAACCTGGA GCCCACAGAG 
AATCTAGGCT CTAGCACACG ACGGCGAGGC CGTAAGGACA AAGAACCATC GCGGGACGAG 
AGCGGACAAA ATCAGAACCT CACTATGGAC TGTGACGGGT CCAAGGGGAA AACGCTTAAT 
GACGCCAAAC AGAGAAAACT CTCTCCTCTC AGGCTCTCAA TATCTAACAA TCAGGATCCT 
GAGTTAATAG AGGATCTAGA AGAGAAAACC TCCGTTAGCA ATGAAGTAGA GATGGAATCA 
GAGGAGCAGA TTGCAGAAAG GAGGAGGAAG ATGGGCAGCC CGGCAGAGGA GTCCCACCGT 
CACGACGCAG GGGCTTCCTG CTGCAGAGCT CTGAGAAACA AGGAGACACG GGAAGAGAGG 
AAGATGGAGG CCATTCTGCA GGCGTTTGCT CGTATGGAGA AGAGGGAAAA GCGGAGGGAG 
CAAGCGTTGG AGAGGATTGG CACCAAAGGG GAGGTTGGTG GACGCAGCGA TATAAAGGAG 
GAGGCACCTG CAACCCCTGA GGCTGAATCT CCTGCCGTGA TACAGCCGCT GCTAGAGGTA 
GTAAAGGAGG AGCCTGGTCT GAAACCGGCG AAGGTGAGCC GTAACAAACA GAGAAAAAGC 
TTCTCCCGGA ACCGGACGCA CATAGGACAG CAGAGGCGGC GAGCACGAAC TGTCAGCACC 
TGCTCCGACT TGCCCCCCAG TTCCCCTGGT GACACTTTAG AGCCCTTAAT GGCAGAGACT 
CAAGATATAG AGATGCCTGT GGCCCCTGAA GCCGATGCTC CGCCAACGCA ACCCCCTGAA 
ACAAGCCCAC CCCACAGTGG CTCACCAGCC CCAACATGCC GCAGTGGACA GAAGTACCCC 
AAAACTAAAA AGCACTTAGT GAGTGAGTGG ATGGGTGTTG ACAAACAGGA GCGGGTTGCG 
TCACGCACTC CGGAGCCTCC CCCTGAAAGG CCGTTGCGGA TCAGTAGTGA TCCTGAAGTC 
TTAGCCACAC AACTGAACTC TTTACCGGGC ATGTCCTGCA GCTCACACGT CTACAGCACG 
CCAAAACACT ACGTCCGCTT TTCTTCTCCT TTCCTGGCCA ACCGTAGTCC CAGTACTCCT 
GGAGTGCCCA CGGGACGACG GCGCTCGAGA GAGATGCCTG AAACGCCAAC TTCCACTGGC 
TCCTGCAAGA AGCGCTGGCT AAAGCAGGCT TTAGAAGAAG AAGGTTCGAC CAGCCCAGGT 
GGAGGACGAC CTTTTCTGCT CATACCCAGT GAGAGTCCTC TCAGTCCCTC TATAAACGGC 
GAGTCCTGCA GCCCTCTACC CCTCAATGGA AGTTGCTCAT TACCAGAGTT GCCTACACCG 
TTGAAGAAGA GGCGCTTGTG TTCACTCGAT CCCTGCATGT CGGAGACTTC CACCCCATAT 
GGGTCTCCAT GTGCCACGCC AACCCGAACA GAGTCAACAG AAGCACCGGG CACACCTTCG 
CTGCTAGCAA CGCCTCCTCG ACCTCGACCA GAGGAGCCCA GTACCGAGCC CTCGACACCC 
TTGCAGATGC CCAACCACCC TCTGTTACAG GAGAGTGAAT CGTCAATGGA CAGTTCTCCA 
GATGGCAGCC GGAGGCCCAG TACACAAGAT GTTGAGCGAC CTCCTTCGCT GCTGGCATCT 
CCCAGCGTGA GAAATGTGAG CTCTGATACG GCTCCACAGG AGTCTAGTAA ATCTATGGGG 
TCTCTGAGTC CTCAGCCCTC CCACACGGAG CCCCAGGATG CCGCAGTGGA TGAAGGCATG 
GAGGTTGATG CTGCTGCTGC TGTTACTTCT GCTGCTGGTG GTGGTGGTGG TTCAGAAGCA 
TCAACATCAG CTCCTGAAAC GCCAGTCTCC TCTTACCCTC CATGGATGAA GAGTCCAGAG 
CGGGGCGGCA TCTCTTTTTC CCCAGTGAAT TCAAATCTGA GAGACCTCAC TCCTTCACAC 
ACCCTGGAGA TGGGGGCGTA CAGGCCGGAT TCAACCTCCA GTGGCCCTTT TAGTGAAGCC 
GCACCCTTCT TTTCTTGCAA TGAGGAGAGC AGTGGTGTGA CCTTTACCCG CTCGCTAAGT 
GGAGATGGCA CAGGGGAGGG AAGCACTGCA AAAAACCCAC AGAAGAAAAA GGTCTCTCTG 
TTGGAGTACA GAAAGCGTCA GCGTGAGGCA CGGCGGAGCG GCTCCAAAGG CGAGTGTAGC 
TCCCCTGTTT CCACAGCACC ACCAGTAGAC GTGTTCCCTG TGGCGGTGGA AATGGCTGTT 
GATCCTCCTG CTCCTGCAGC AACACCGACC CCCAGGACCC CGCAGCCTAG TGAGGATCCA 
GACGCCCCTC AGGGGGAGAG AGATGGAGAA GGCCAGTGGA CATCTTCCAC ATCAGTAGAG 
CAGGCCAGGG AGCGGGGCTA CCACAGGGCC TTATTGCTGA GTGATCATCG CAAAGATACA 
GATGGTGGAG AGTCAGAAGG TGGTGATTCC CAAGTGAAGG ACTGTTCTTC TCCCAAGAGC 
TGCAAGAGCC CCTCGACACA TGCACCTTGT TCTCCAGCAG CCCAGGCTGT GTCTCGGCCA 
TCAAAGGATG AAGATGAAGC ACAGACTCGT GGTCCTGCTC AGCCTCAGGC AGTGTCCGTC 
CAGCCGTCCA GCGCTAAGAC TCCCAGCTCA AAACCAGCTG CTCTGACGCC CAGCAAGCTC 
CACTGTGGGC CCTCAGGCGC CTCTCAGAGT CACTACGCGG GACCTTCGCT CATGCACTCT 
CCCAAAGCGC AACCCCAAGG ATCACCTTTC CGTGGCCAGC GAGCCTTTCT GACAGCTCAG 
CCTCAGAACC AGCCTCAACC ACAAGCTACA TCTGGCCCTG CAACTTTCCC TCAGTATAAC 
CCTCAAAATG CACCTCCACC TCCGCCCCCT CCTCCCCCAG CTCCACCTAC ATCTGCTCCT 
TACTTCCCCA CCCAGCCTAC TGCTGCTGCT GCTCCCTTTC CTGCATTTAA GCCCACCGTA 
GCCTCGCCCT TTCCTCCTGG TTCCCAGCCT CTACTTCAGA CCCATCATGC ACTGCATTAC 
CAAAGCAGTG CTGCTCCTCC TCCTCCACCA CCTCCTCCCC CTCCCCACCC GCAGCCTGGC 
CCCACCTTAC TGCATGTCAA TCTGCAACCT CCTTCACTGC AGCAGCATCA GCTCCTGCTG 
AGCTCCGCCC CACCTCCGCC TCCTCCTCCA CCTCCTCAGA CACAGAGTTC CCAGCAGCCC 
CCGCCTAACT CCGGCACGCT CCTGTCAATC AAACAAGGAC CACACCCACC CCTTCCACCC 
CCTCCTCCAG CTCCCTCCAC TAACGCACCA CCGCACCCGT TCCAAAACAT CAGTGGCTTT 
CAAACCACTC TACTCCACCA GTCTGCACCA GCCAACCCCT CAGTAACCGC ATCTACTTAT 
CAACAAACTG TATTACCCCC TCCTCCTCCA CCTCCCCCCC AACAAACTCC TCCCACACAG 
ACCCCGCCCA ATCCGAGCAT CCCTCAGATC GCAGGTGGCA ATCGAGGACC CGCTCCTTCC 
TCCGCCTCTT TCCACACCAC CGGATACTTG GGCACAGGAT GGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_009300441.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298716.1 Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X3, mRNA.

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CloneID	ODa39844
Clone ID Related Accession (Same CDS sequence)	XM_009300440.2 , XM_009300439.2 , XM_009300441.2 , XM_009300439.3 , XM_021475266.1
Accession Version	XM_009300441.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	histone-lysine N-methyltransferase 2E isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300441.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACGGCCAACA CCTCGTATCT GGACATGGCT 
GCAGGCTCAG AACCAGAATC TGTGGAGGCG AGCTCTGTGG TAGTTGAGAA GTCCACCTAC 
CCTCACCAGA TTTACAGCAG CAGCTCCCAC CACTCCCACG GATACATCGG TCTGCCTTAC 
GCCGACCATA ATTATGGGGC GCGACCCCCA CCCACCCCGC CGGCGTCCCC ACCTCCCTCG 
GTGCTGATCC GGCCGGGCGA GAGCTTGTTT GTGTCCGGAA GCCGGCCTGG TGAGAACTTG 
TTTGTGCCGG GCGGGCAGGA CGAGGCGTCG CGTGGCACCA CGCTCAGCAC CTCGGAGGAC 
GGCAGCTACG GTGCTGACAT CACACGCTGC ATCTGTGGCT TCACCCACGA CGATGGCTAC 
ATGATCTGCT GCGACAAGTG CAGTGTGTGG CAGCACATAG ACTGCATGGG GATCGACAGG 
CAGCACATTC CCGAAACCTA CCTGTGTGAG CGCTGTCAGC CACGCACCCT GGACAGAGAG 
CACGCCATCC TCCTGCAAAC CAGGAAGAGA GAGAACATGT CTGATGGGGA CACCAGTGCT 
ACAGAAAGCG GAGATGAGGT TCCACTAGAG CTGTACACAG CGTTTCAGCA CACCCCTACC 
AGCATCACAC TCACAACAGC ACGTCTGGGT AACAAACAGG CTGATAAGAA ACGAAAGAAG 
AGCGGAGAGA AGGAGCCGCC GGCTACAGCC AGGGCCAAGA AGTCATTCCG TGAAGGCTCT 
AGAAAATCAT CTAGAGTAAA GGGCTCCGCT CCCGAGTGTG AGCCGATTGA TCCTCCCTCT 
CTATGGGAGA ATAAGATGAA GTCGTGGATG GAGCGCTACG AGGAGGCCAG CAGTAACCAG 
TACAGTGAAG AAGTGCGGCT ACTGCTGAGA GTCAAAGAAG CCCGAGATGG AAAAACCCTG 
GCCTACAATA CACACACCGC AGCCTTCAAG CCACCTGTAG AGAGTCAAGT ACAAAAAAAC 
AAGCGAATTC TGAAGGCTGT GCGGGATTTG GCTGCTGATT CATTAATAAT CGAGTACAGG 
GGGAAGGTCA TGCTAAGACA GCAGTTTGAG GCTAATGGCT ACTTCTTTAA GAGGCCATAC 
CCTTTTGTAT TGTTCTACTC TAAGTTTGAC GGTTTGGAAA TGTGCGTAGA CGCACGGAGC 
TTTGGGAATG AGGCCCGATT CATTCGGCGC TCCTGCACCC CTAACGCTGA AGTGCGGCAC 
GTGATTGAAG AGGGCATGCT GCATTTATAC ATTTACTCTC TGAGGTCCAT CTCCAAAGGG 
AGCGAGATCA CCATTGGCTT TGATTACGAC TACGGCAGCT GTAAATATAA GGTGGACTGT 
GCTTGTGTAA AAGGCAATCC GGAGTGTCCT GTGCTCAAGC ATAACCTGGA GCCCACAGAG 
AATCTAGGCT CTAGCACACG ACGGCGAGGC CGTAAGGACA AAGAACCATC GCGGGACGAG 
AGCGGACAAA ATCAGAACCT CACTATGGAC TGTGACGGGT CCAAGGGGAA AACGCTTAAT 
GACGCCAAAC AGAGAAAACT CTCTCCTCTC AGGCTCTCAA TATCTAACAA TCAGGATCCT 
GAGTTAATAG AGGATCTAGA AGAGAAAACC TCCGTTAGCA ATGAAGTAGA GATGGAATCA 
GAGGAGCAGA TTGCAGAAAG GAGGAGGAAG ATGGGCAGCC CGGCAGAGGA GTCCCACCGT 
CACGACGCAG GGGCTTCCTG CTGCAGAGCT CTGAGAAACA AGGAGACACG GGAAGAGAGG 
AAGATGGAGG CCATTCTGCA GGCGTTTGCT CGTATGGAGA AGAGGGAAAA GCGGAGGGAG 
CAAGCGTTGG AGAGGATTGG CACCAAAGGG GAGGTTGGTG GACGCAGCGA TATAAAGGAG 
GAGGCACCTG CAACCCCTGA GGCTGAATCT CCTGCCGTGA TACAGCCGCT GCTAGAGGTA 
GTAAAGGAGG AGCCTGGTCT GAAACCGGCG AAGGTGAGCC GTAACAAACA GAGAAAAAGC 
TTCTCCCGGA ACCGGACGCA CATAGGACAG CAGAGGCGGC GAGCACGAAC TGTCAGCACC 
TGCTCCGACT TGCCCCCCAG TTCCCCTGGT GACACTTTAG AGCCCTTAAT GGCAGAGACT 
CAAGATATAG AGATGCCTGT GGCCCCTGAA GCCGATGCTC CGCCAACGCA ACCCCCTGAA 
ACAAGCCCAC CCCACAGTGG CTCACCAGCC CCAACATGCC GCAGTGGACA GAAGTACCCC 
AAAACTAAAA AGCACTTAGT GAGTGAGTGG ATGGGTGTTG ACAAACAGGA GCGGGTTGCG 
TCACGCACTC CGGAGCCTCC CCCTGAAAGG CCGTTGCGGA TCAGTAGTGA TCCTGAAGTC 
TTAGCCACAC AACTGAACTC TTTACCGGGC ATGTCCTGCA GCTCACACGT CTACAGCACG 
CCAAAACACT ACGTCCGCTT TTCTTCTCCT TTCCTGGCCA ACCGTAGTCC CAGTACTCCT 
GGAGTGCCCA CGGGACGACG GCGCTCGAGA GAGATGCCTG AAACGCCAAC TTCCACTGGC 
TCCTGCAAGA AGCGCTGGCT AAAGCAGGCT TTAGAAGAAG AAGGTTCGAC CAGCCCAGGT 
GGAGGACGAC CTTTTCTGCT CATACCCAGT GAGAGTCCTC TCAGTCCCTC TATAAACGGC 
GAGTCCTGCA GCCCTCTACC CCTCAATGGA AGTTGCTCAT TACCAGAGTT GCCTACACCG 
TTGAAGAAGA GGCGCTTGTG TTCACTCGAT CCCTGCATGT CGGAGACTTC CACCCCATAT 
GGGTCTCCAT GTGCCACGCC AACCCGAACA GAGTCAACAG AAGCACCGGG CACACCTTCG 
CTGCTAGCAA CGCCTCCTCG ACCTCGACCA GAGGAGCCCA GTACCGAGCC CTCGACACCC 
TTGCAGATGC CCAACCACCC TCTGTTACAG GAGAGTGAAT CGTCAATGGA CAGTTCTCCA 
GATGGCAGCC GGAGGCCCAG TACACAAGAT GTTGAGCGAC CTCCTTCGCT GCTGGCATCT 
CCCAGCGTGA GAAATGTGAG CTCTGATACG GCTCCACAGG AGTCTAGTAA ATCTATGGGG 
TCTCTGAGTC CTCAGCCCTC CCACACGGAG CCCCAGGATG CCGCAGTGGA TGAAGGCATG 
GAGGTTGATG CTGCTGCTGC TGTTACTTCT GCTGCTGGTG GTGGTGGTGG TTCAGAAGCA 
TCAACATCAG CTCCTGAAAC GCCAGTCTCC TCTTACCCTC CATGGATGAA GAGTCCAGAG 
CGGGGCGGCA TCTCTTTTTC CCCAGTGAAT TCAAATCTGA GAGACCTCAC TCCTTCACAC 
ACCCTGGAGA TGGGGGCGTA CAGGCCGGAT TCAACCTCCA GTGGCCCTTT TAGTGAAGCC 
GCACCCTTCT TTTCTTGCAA TGAGGAGAGC AGTGGTGTGA CCTTTACCCG CTCGCTAAGT 
GGAGATGGCA CAGGGGAGGG AAGCACTGCA AAAAACCCAC AGAAGAAAAA GGTCTCTCTG 
TTGGAGTACA GAAAGCGTCA GCGTGAGGCA CGGCGGAGCG GCTCCAAAGG CGAGTGTAGC 
TCCCCTGTTT CCACAGCACC ACCAGTAGAC GTGTTCCCTG TGGCGGTGGA AATGGCTGTT 
GATCCTCCTG CTCCTGCAGC AACACCGACC CCCAGGACCC CGCAGCCTAG TGAGGATCCA 
GACGCCCCTC AGGGGGAGAG AGATGGAGAA GGCCAGTGGA CATCTTCCAC ATCAGTAGAG 
CAGGCCAGGG AGCGGGGCTA CCACAGGGCC TTATTGCTGA GTGATCATCG CAAAGATACA 
GATGGTGGAG AGTCAGAAGG TGGTGATTCC CAAGTGAAGG ACTGTTCTTC TCCCAAGAGC 
TGCAAGAGCC CCTCGACACA TGCACCTTGT TCTCCAGCAG CCCAGGCTGT GTCTCGGCCA 
TCAAAGGATG AAGATGAAGC ACAGACTCGT GGTCCTGCTC AGCCTCAGGC AGTGTCCGTC 
CAGCCGTCCA GCGCTAAGAC TCCCAGCTCA AAACCAGCTG CTCTGACGCC CAGCAAGCTC 
CACTGTGGGC CCTCAGGCGC CTCTCAGAGT CACTACGCGG GACCTTCGCT CATGCACTCT 
CCCAAAGCGC AACCCCAAGG ATCACCTTTC CGTGGCCAGC GAGCCTTTCT GACAGCTCAG 
CCTCAGAACC AGCCTCAACC ACAAGCTACA TCTGGCCCTG CAACTTTCCC TCAGTATAAC 
CCTCAAAATG CACCTCCACC TCCGCCCCCT CCTCCCCCAG CTCCACCTAC ATCTGCTCCT 
TACTTCCCCA CCCAGCCTAC TGCTGCTGCT GCTCCCTTTC CTGCATTTAA GCCCACCGTA 
GCCTCGCCCT TTCCTCCTGG TTCCCAGCCT CTACTTCAGA CCCATCATGC ACTGCATTAC 
CAAAGCAGTG CTGCTCCTCC TCCTCCACCA CCTCCTCCCC CTCCCCACCC GCAGCCTGGC 
CCCACCTTAC TGCATGTCAA TCTGCAACCT CCTTCACTGC AGCAGCATCA GCTCCTGCTG 
AGCTCCGCCC CACCTCCGCC TCCTCCTCCA CCTCCTCAGA CACAGAGTTC CCAGCAGCCC 
CCGCCTAACT CCGGCACGCT CCTGTCAATC AAACAAGGAC CACACCCACC CCTTCCACCC 
CCTCCTCCAG CTCCCTCCAC TAACGCACCA CCGCACCCGT TCCAAAACAT CAGTGGCTTT 
CAAACCACTC TACTCCACCA GTCTGCACCA GCCAACCCCT CAGTAACCGC ATCTACTTAT 
CAACAAACTG TATTACCCCC TCCTCCTCCA CCTCCCCCCC AACAAACTCC TCCCACACAG 
ACCCCGCCCA ATCCGAGCAT CCCTCAGATC GCAGGTGGCA ATCGAGGACC CGCTCCTTCC 
TCCGCCTCTT TCCACACCAC CGGATACTTG GGCACAGGAT GGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298716.1
CDS	134..5101
Translation	MSIVIPVGVDTANTSYLDMAAGSEPESVEASSVVVEKSTYPHQIYSSSSHHSHGYIGLPYADHNYGARPPPTPPASPPPSVLIRPGESLFVSGSRPGENLFVPGGQDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQHIPETYLCERCQPRTLDREHAILLQTRKRENMSDGDTSATESGDEVPLELYTAFQHTPTSITLTTARLGNKQADKKRKKSGEKEPPATARAKKSFREGSRKSSRVKGSAPECEPIDPPSLWENKMKSWMERYEEASSNQYSEEVRLLLRVKEARDGKTLAYNTHTAAFKPPVESQVQKNKRILKAVRDLAADSLIIEYRGKVMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARSFGNEARFIRRSCTPNAEVRHVIEEGMLHLYIYSLRSISKGSEITIGFDYDYGSCKYKVDCACVKGNPECPVLKHNLEPTENLGSSTRRRGRKDKEPSRDESGQNQNLTMDCDGSKGKTLNDAKQRKLSPLRLSISNNQDPELIEDLEEKTSVSNEVEMESEEQIAERRRKMGSPAEESHRHDAGASCCRALRNKETREERKMEAILQAFARMEKREKRREQALERIGTKGEVGGRSDIKEEAPATPEAESPAVIQPLLEVVKEEPGLKPAKVSRNKQRKSFSRNRTHIGQQRRRARTVSTCSDLPPSSPGDTLEPLMAETQDIEMPVAPEADAPPTQPPETSPPHSGSPAPTCRSGQKYPKTKKHLVSEWMGVDKQERVASRTPEPPPERPLRISSDPEVLATQLNSLPGMSCSSHVYSTPKHYVRFSSPFLANRSPSTPGVPTGRRRSREMPETPTSTGSCKKRWLKQALEEEGSTSPGGGRPFLLIPSESPLSPSINGESCSPLPLNGSCSLPELPTPLKKRRLCSLDPCMSETSTPYGSPCATPTRTESTEAPGTPSLLATPPRPRPEEPSTEPSTPLQMPNHPLLQESESSMDSSPDGSRRPSTQDVERPPSLLASPSVRNVSSDTAPQESSKSMGSLSPQPSHTEPQDAAVDEGMEVDAAAAVTSAAGGGGGSEASTSAPETPVSSYPPWMKSPERGGISFSPVNSNLRDLTPSHTLEMGAYRPDSTSSGPFSEAAPFFSCNEESSGVTFTRSLSGDGTGEGSTAKNPQKKKVSLLEYRKRQREARRSGSKGECSSPVSTAPPVDVFPVAVEMAVDPPAPAATPTPRTPQPSEDPDAPQGERDGEGQWTSSTSVEQARERGYHRALLLSDHRKDTDGGESEGGDSQVKDCSSPKSCKSPSTHAPCSPAAQAVSRPSKDEDEAQTRGPAQPQAVSVQPSSAKTPSSKPAALTPSKLHCGPSGASQSHYAGPSLMHSPKAQPQGSPFRGQRAFLTAQPQNQPQPQATSGPATFPQYNPQNAPPPPPPPPPAPPTSAPYFPTQPTAAAAPFPAFKPTVASPFPPGSQPLLQTHHALHYQSSAAPPPPPPPPPPHPQPGPTLLHVNLQPPSLQQHQLLLSSAPPPPPPPPPQTQSSQQPPPNSGTLLSIKQGPHPPLPPPPPAPSTNAPPHPFQNISGFQTTLLHQSAPANPSVTASTYQQTVLPPPPPPPPQQTPPTQTPPNPSIPQIAGGNRGPAPSSASFHTTGYLGTGWH

Target ORF information:

RefSeq Version	XM_009300441.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300441.2

ORF Insert Sequence:

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACGGCCAACA CCTCGTATCT GGACATGGCT 
GCAGGCTCAG AACCAGAATC TGTGGAGGCG AGCTCTGTGG TAGTTGAGAA GTCCACCTAC 
CCTCACCAGA TTTACAGCAG CAGCTCCCAC CACTCCCACG GATACATCGG TCTGCCTTAC 
GCCGACCATA ATTATGGGGC GCGACCCCCA CCCACCCCGC CGGCGTCCCC ACCTCCCTCG 
GTGCTGATCC GGCCGGGCGA GAGCTTGTTT GTGTCCGGAA GCCGGCCTGG TGAGAACTTG 
TTTGTGCCGG GCGGGCAGGA CGAGGCGTCG CGTGGCACCA CGCTCAGCAC CTCGGAGGAC 
GGCAGCTACG GTGCTGACAT CACACGCTGC ATCTGTGGCT TCACCCACGA CGATGGCTAC 
ATGATCTGCT GCGACAAGTG CAGTGTGTGG CAGCACATAG ACTGCATGGG GATCGACAGG 
CAGCACATTC CCGAAACCTA CCTGTGTGAG CGCTGTCAGC CACGCACCCT GGACAGAGAG 
CACGCCATCC TCCTGCAAAC CAGGAAGAGA GAGAACATGT CTGATGGGGA CACCAGTGCT 
ACAGAAAGCG GAGATGAGGT TCCACTAGAG CTGTACACAG CGTTTCAGCA CACCCCTACC 
AGCATCACAC TCACAACAGC ACGTCTGGGT AACAAACAGG CTGATAAGAA ACGAAAGAAG 
AGCGGAGAGA AGGAGCCGCC GGCTACAGCC AGGGCCAAGA AGTCATTCCG TGAAGGCTCT 
AGAAAATCAT CTAGAGTAAA GGGCTCCGCT CCCGAGTGTG AGCCGATTGA TCCTCCCTCT 
CTATGGGAGA ATAAGATGAA GTCGTGGATG GAGCGCTACG AGGAGGCCAG CAGTAACCAG 
TACAGTGAAG AAGTGCGGCT ACTGCTGAGA GTCAAAGAAG CCCGAGATGG AAAAACCCTG 
GCCTACAATA CACACACCGC AGCCTTCAAG CCACCTGTAG AGAGTCAAGT ACAAAAAAAC 
AAGCGAATTC TGAAGGCTGT GCGGGATTTG GCTGCTGATT CATTAATAAT CGAGTACAGG 
GGGAAGGTCA TGCTAAGACA GCAGTTTGAG GCTAATGGCT ACTTCTTTAA GAGGCCATAC 
CCTTTTGTAT TGTTCTACTC TAAGTTTGAC GGTTTGGAAA TGTGCGTAGA CGCACGGAGC 
TTTGGGAATG AGGCCCGATT CATTCGGCGC TCCTGCACCC CTAACGCTGA AGTGCGGCAC 
GTGATTGAAG AGGGCATGCT GCATTTATAC ATTTACTCTC TGAGGTCCAT CTCCAAAGGG 
AGCGAGATCA CCATTGGCTT TGATTACGAC TACGGCAGCT GTAAATATAA GGTGGACTGT 
GCTTGTGTAA AAGGCAATCC GGAGTGTCCT GTGCTCAAGC ATAACCTGGA GCCCACAGAG 
AATCTAGGCT CTAGCACACG ACGGCGAGGC CGTAAGGACA AAGAACCATC GCGGGACGAG 
AGCGGACAAA ATCAGAACCT CACTATGGAC TGTGACGGGT CCAAGGGGAA AACGCTTAAT 
GACGCCAAAC AGAGAAAACT CTCTCCTCTC AGGCTCTCAA TATCTAACAA TCAGGATCCT 
GAGTTAATAG AGGATCTAGA AGAGAAAACC TCCGTTAGCA ATGAAGTAGA GATGGAATCA 
GAGGAGCAGA TTGCAGAAAG GAGGAGGAAG ATGGGCAGCC CGGCAGAGGA GTCCCACCGT 
CACGACGCAG GGGCTTCCTG CTGCAGAGCT CTGAGAAACA AGGAGACACG GGAAGAGAGG 
AAGATGGAGG CCATTCTGCA GGCGTTTGCT CGTATGGAGA AGAGGGAAAA GCGGAGGGAG 
CAAGCGTTGG AGAGGATTGG CACCAAAGGG GAGGTTGGTG GACGCAGCGA TATAAAGGAG 
GAGGCACCTG CAACCCCTGA GGCTGAATCT CCTGCCGTGA TACAGCCGCT GCTAGAGGTA 
GTAAAGGAGG AGCCTGGTCT GAAACCGGCG AAGGTGAGCC GTAACAAACA GAGAAAAAGC 
TTCTCCCGGA ACCGGACGCA CATAGGACAG CAGAGGCGGC GAGCACGAAC TGTCAGCACC 
TGCTCCGACT TGCCCCCCAG TTCCCCTGGT GACACTTTAG AGCCCTTAAT GGCAGAGACT 
CAAGATATAG AGATGCCTGT GGCCCCTGAA GCCGATGCTC CGCCAACGCA ACCCCCTGAA 
ACAAGCCCAC CCCACAGTGG CTCACCAGCC CCAACATGCC GCAGTGGACA GAAGTACCCC 
AAAACTAAAA AGCACTTAGT GAGTGAGTGG ATGGGTGTTG ACAAACAGGA GCGGGTTGCG 
TCACGCACTC CGGAGCCTCC CCCTGAAAGG CCGTTGCGGA TCAGTAGTGA TCCTGAAGTC 
TTAGCCACAC AACTGAACTC TTTACCGGGC ATGTCCTGCA GCTCACACGT CTACAGCACG 
CCAAAACACT ACGTCCGCTT TTCTTCTCCT TTCCTGGCCA ACCGTAGTCC CAGTACTCCT 
GGAGTGCCCA CGGGACGACG GCGCTCGAGA GAGATGCCTG AAACGCCAAC TTCCACTGGC 
TCCTGCAAGA AGCGCTGGCT AAAGCAGGCT TTAGAAGAAG AAGGTTCGAC CAGCCCAGGT 
GGAGGACGAC CTTTTCTGCT CATACCCAGT GAGAGTCCTC TCAGTCCCTC TATAAACGGC 
GAGTCCTGCA GCCCTCTACC CCTCAATGGA AGTTGCTCAT TACCAGAGTT GCCTACACCG 
TTGAAGAAGA GGCGCTTGTG TTCACTCGAT CCCTGCATGT CGGAGACTTC CACCCCATAT 
GGGTCTCCAT GTGCCACGCC AACCCGAACA GAGTCAACAG AAGCACCGGG CACACCTTCG 
CTGCTAGCAA CGCCTCCTCG ACCTCGACCA GAGGAGCCCA GTACCGAGCC CTCGACACCC 
TTGCAGATGC CCAACCACCC TCTGTTACAG GAGAGTGAAT CGTCAATGGA CAGTTCTCCA 
GATGGCAGCC GGAGGCCCAG TACACAAGAT GTTGAGCGAC CTCCTTCGCT GCTGGCATCT 
CCCAGCGTGA GAAATGTGAG CTCTGATACG GCTCCACAGG AGTCTAGTAA ATCTATGGGG 
TCTCTGAGTC CTCAGCCCTC CCACACGGAG CCCCAGGATG CCGCAGTGGA TGAAGGCATG 
GAGGTTGATG CTGCTGCTGC TGTTACTTCT GCTGCTGGTG GTGGTGGTGG TTCAGAAGCA 
TCAACATCAG CTCCTGAAAC GCCAGTCTCC TCTTACCCTC CATGGATGAA GAGTCCAGAG 
CGGGGCGGCA TCTCTTTTTC CCCAGTGAAT TCAAATCTGA GAGACCTCAC TCCTTCACAC 
ACCCTGGAGA TGGGGGCGTA CAGGCCGGAT TCAACCTCCA GTGGCCCTTT TAGTGAAGCC 
GCACCCTTCT TTTCTTGCAA TGAGGAGAGC AGTGGTGTGA CCTTTACCCG CTCGCTAAGT 
GGAGATGGCA CAGGGGAGGG AAGCACTGCA AAAAACCCAC AGAAGAAAAA GGTCTCTCTG 
TTGGAGTACA GAAAGCGTCA GCGTGAGGCA CGGCGGAGCG GCTCCAAAGG CGAGTGTAGC 
TCCCCTGTTT CCACAGCACC ACCAGTAGAC GTGTTCCCTG TGGCGGTGGA AATGGCTGTT 
GATCCTCCTG CTCCTGCAGC AACACCGACC CCCAGGACCC CGCAGCCTAG TGAGGATCCA 
GACGCCCCTC AGGGGGAGAG AGATGGAGAA GGCCAGTGGA CATCTTCCAC ATCAGTAGAG 
CAGGCCAGGG AGCGGGGCTA CCACAGGGCC TTATTGCTGA GTGATCATCG CAAAGATACA 
GATGGTGGAG AGTCAGAAGG TGGTGATTCC CAAGTGAAGG ACTGTTCTTC TCCCAAGAGC 
TGCAAGAGCC CCTCGACACA TGCACCTTGT TCTCCAGCAG CCCAGGCTGT GTCTCGGCCA 
TCAAAGGATG AAGATGAAGC ACAGACTCGT GGTCCTGCTC AGCCTCAGGC AGTGTCCGTC 
CAGCCGTCCA GCGCTAAGAC TCCCAGCTCA AAACCAGCTG CTCTGACGCC CAGCAAGCTC 
CACTGTGGGC CCTCAGGCGC CTCTCAGAGT CACTACGCGG GACCTTCGCT CATGCACTCT 
CCCAAAGCGC AACCCCAAGG ATCACCTTTC CGTGGCCAGC GAGCCTTTCT GACAGCTCAG 
CCTCAGAACC AGCCTCAACC ACAAGCTACA TCTGGCCCTG CAACTTTCCC TCAGTATAAC 
CCTCAAAATG CACCTCCACC TCCGCCCCCT CCTCCCCCAG CTCCACCTAC ATCTGCTCCT 
TACTTCCCCA CCCAGCCTAC TGCTGCTGCT GCTCCCTTTC CTGCATTTAA GCCCACCGTA 
GCCTCGCCCT TTCCTCCTGG TTCCCAGCCT CTACTTCAGA CCCATCATGC ACTGCATTAC 
CAAAGCAGTG CTGCTCCTCC TCCTCCACCA CCTCCTCCCC CTCCCCACCC GCAGCCTGGC 
CCCACCTTAC TGCATGTCAA TCTGCAACCT CCTTCACTGC AGCAGCATCA GCTCCTGCTG 
AGCTCCGCCC CACCTCCGCC TCCTCCTCCA CCTCCTCAGA CACAGAGTTC CCAGCAGCCC 
CCGCCTAACT CCGGCACGCT CCTGTCAATC AAACAAGGAC CACACCCACC CCTTCCACCC 
CCTCCTCCAG CTCCCTCCAC TAACGCACCA CCGCACCCGT TCCAAAACAT CAGTGGCTTT 
CAAACCACTC TACTCCACCA GTCTGCACCA GCCAACCCCT CAGTAACCGC ATCTACTTAT 
CAACAAACTG TATTACCCCC TCCTCCTCCA CCTCCCCCCC AACAAACTCC TCCCACACAG 
ACCCCGCCCA ATCCGAGCAT CCCTCAGATC GCAGGTGGCA ATCGAGGACC CGCTCCTTCC 
TCCGCCTCTT TCCACACCAC CGGATACTTG GGCACAGGAT GGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_009300439.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298714.1 Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X1, mRNA.

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CloneID	ODa39844
Clone ID Related Accession (Same CDS sequence)	XM_009300440.2 , XM_009300439.2 , XM_009300441.2 , XM_009300439.3 , XM_021475266.1
Accession Version	XM_009300439.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	histone-lysine N-methyltransferase 2E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300439.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACGGCCAACA CCTCGTATCT GGACATGGCT 
GCAGGCTCAG AACCAGAATC TGTGGAGGCG AGCTCTGTGG TAGTTGAGAA GTCCACCTAC 
CCTCACCAGA TTTACAGCAG CAGCTCCCAC CACTCCCACG GATACATCGG TCTGCCTTAC 
GCCGACCATA ATTATGGGGC GCGACCCCCA CCCACCCCGC CGGCGTCCCC ACCTCCCTCG 
GTGCTGATCC GGCCGGGCGA GAGCTTGTTT GTGTCCGGAA GCCGGCCTGG TGAGAACTTG 
TTTGTGCCGG GCGGGCAGGA CGAGGCGTCG CGTGGCACCA CGCTCAGCAC CTCGGAGGAC 
GGCAGCTACG GTGCTGACAT CACACGCTGC ATCTGTGGCT TCACCCACGA CGATGGCTAC 
ATGATCTGCT GCGACAAGTG CAGTGTGTGG CAGCACATAG ACTGCATGGG GATCGACAGG 
CAGCACATTC CCGAAACCTA CCTGTGTGAG CGCTGTCAGC CACGCACCCT GGACAGAGAG 
CACGCCATCC TCCTGCAAAC CAGGAAGAGA GAGAACATGT CTGATGGGGA CACCAGTGCT 
ACAGAAAGCG GAGATGAGGT TCCACTAGAG CTGTACACAG CGTTTCAGCA CACCCCTACC 
AGCATCACAC TCACAACAGC ACGTCTGGGT AACAAACAGG CTGATAAGAA ACGAAAGAAG 
AGCGGAGAGA AGGAGCCGCC GGCTACAGCC AGGGCCAAGA AGTCATTCCG TGAAGGCTCT 
AGAAAATCAT CTAGAGTAAA GGGCTCCGCT CCCGAGTGTG AGCCGATTGA TCCTCCCTCT 
CTATGGGAGA ATAAGATGAA GTCGTGGATG GAGCGCTACG AGGAGGCCAG CAGTAACCAG 
TACAGTGAAG AAGTGCGGCT ACTGCTGAGA GTCAAAGAAG CCCGAGATGG AAAAACCCTG 
GCCTACAATA CACACACCGC AGCCTTCAAG CCACCTGTAG AGAGTCAAGT ACAAAAAAAC 
AAGCGAATTC TGAAGGCTGT GCGGGATTTG GCTGCTGATT CATTAATAAT CGAGTACAGG 
GGGAAGGTCA TGCTAAGACA GCAGTTTGAG GCTAATGGCT ACTTCTTTAA GAGGCCATAC 
CCTTTTGTAT TGTTCTACTC TAAGTTTGAC GGTTTGGAAA TGTGCGTAGA CGCACGGAGC 
TTTGGGAATG AGGCCCGATT CATTCGGCGC TCCTGCACCC CTAACGCTGA AGTGCGGCAC 
GTGATTGAAG AGGGCATGCT GCATTTATAC ATTTACTCTC TGAGGTCCAT CTCCAAAGGG 
AGCGAGATCA CCATTGGCTT TGATTACGAC TACGGCAGCT GTAAATATAA GGTGGACTGT 
GCTTGTGTAA AAGGCAATCC GGAGTGTCCT GTGCTCAAGC ATAACCTGGA GCCCACAGAG 
AATCTAGGCT CTAGCACACG ACGGCGAGGC CGTAAGGACA AAGAACCATC GCGGGACGAG 
AGCGGACAAA ATCAGAACCT CACTATGGAC TGTGACGGGT CCAAGGGGAA AACGCTTAAT 
GACGCCAAAC AGAGAAAACT CTCTCCTCTC AGGCTCTCAA TATCTAACAA TCAGGATCCT 
GAGTTAATAG AGGATCTAGA AGAGAAAACC TCCGTTAGCA ATGAAGTAGA GATGGAATCA 
GAGGAGCAGA TTGCAGAAAG GAGGAGGAAG ATGGGCAGCC CGGCAGAGGA GTCCCACCGT 
CACGACGCAG GGGCTTCCTG CTGCAGAGCT CTGAGAAACA AGGAGACACG GGAAGAGAGG 
AAGATGGAGG CCATTCTGCA GGCGTTTGCT CGTATGGAGA AGAGGGAAAA GCGGAGGGAG 
CAAGCGTTGG AGAGGATTGG CACCAAAGGG GAGGTTGGTG GACGCAGCGA TATAAAGGAG 
GAGGCACCTG CAACCCCTGA GGCTGAATCT CCTGCCGTGA TACAGCCGCT GCTAGAGGTA 
GTAAAGGAGG AGCCTGGTCT GAAACCGGCG AAGGTGAGCC GTAACAAACA GAGAAAAAGC 
TTCTCCCGGA ACCGGACGCA CATAGGACAG CAGAGGCGGC GAGCACGAAC TGTCAGCACC 
TGCTCCGACT TGCCCCCCAG TTCCCCTGGT GACACTTTAG AGCCCTTAAT GGCAGAGACT 
CAAGATATAG AGATGCCTGT GGCCCCTGAA GCCGATGCTC CGCCAACGCA ACCCCCTGAA 
ACAAGCCCAC CCCACAGTGG CTCACCAGCC CCAACATGCC GCAGTGGACA GAAGTACCCC 
AAAACTAAAA AGCACTTAGT GAGTGAGTGG ATGGGTGTTG ACAAACAGGA GCGGGTTGCG 
TCACGCACTC CGGAGCCTCC CCCTGAAAGG CCGTTGCGGA TCAGTAGTGA TCCTGAAGTC 
TTAGCCACAC AACTGAACTC TTTACCGGGC ATGTCCTGCA GCTCACACGT CTACAGCACG 
CCAAAACACT ACGTCCGCTT TTCTTCTCCT TTCCTGGCCA ACCGTAGTCC CAGTACTCCT 
GGAGTGCCCA CGGGACGACG GCGCTCGAGA GAGATGCCTG AAACGCCAAC TTCCACTGGC 
TCCTGCAAGA AGCGCTGGCT AAAGCAGGCT TTAGAAGAAG AAGGTTCGAC CAGCCCAGGT 
GGAGGACGAC CTTTTCTGCT CATACCCAGT GAGAGTCCTC TCAGTCCCTC TATAAACGGC 
GAGTCCTGCA GCCCTCTACC CCTCAATGGA AGTTGCTCAT TACCAGAGTT GCCTACACCG 
TTGAAGAAGA GGCGCTTGTG TTCACTCGAT CCCTGCATGT CGGAGACTTC CACCCCATAT 
GGGTCTCCAT GTGCCACGCC AACCCGAACA GAGTCAACAG AAGCACCGGG CACACCTTCG 
CTGCTAGCAA CGCCTCCTCG ACCTCGACCA GAGGAGCCCA GTACCGAGCC CTCGACACCC 
TTGCAGATGC CCAACCACCC TCTGTTACAG GAGAGTGAAT CGTCAATGGA CAGTTCTCCA 
GATGGCAGCC GGAGGCCCAG TACACAAGAT GTTGAGCGAC CTCCTTCGCT GCTGGCATCT 
CCCAGCGTGA GAAATGTGAG CTCTGATACG GCTCCACAGG AGTCTAGTAA ATCTATGGGG 
TCTCTGAGTC CTCAGCCCTC CCACACGGAG CCCCAGGATG CCGCAGTGGA TGAAGGCATG 
GAGGTTGATG CTGCTGCTGC TGTTACTTCT GCTGCTGGTG GTGGTGGTGG TTCAGAAGCA 
TCAACATCAG CTCCTGAAAC GCCAGTCTCC TCTTACCCTC CATGGATGAA GAGTCCAGAG 
CGGGGCGGCA TCTCTTTTTC CCCAGTGAAT TCAAATCTGA GAGACCTCAC TCCTTCACAC 
ACCCTGGAGA TGGGGGCGTA CAGGCCGGAT TCAACCTCCA GTGGCCCTTT TAGTGAAGCC 
GCACCCTTCT TTTCTTGCAA TGAGGAGAGC AGTGGTGTGA CCTTTACCCG CTCGCTAAGT 
GGAGATGGCA CAGGGGAGGG AAGCACTGCA AAAAACCCAC AGAAGAAAAA GGTCTCTCTG 
TTGGAGTACA GAAAGCGTCA GCGTGAGGCA CGGCGGAGCG GCTCCAAAGG CGAGTGTAGC 
TCCCCTGTTT CCACAGCACC ACCAGTAGAC GTGTTCCCTG TGGCGGTGGA AATGGCTGTT 
GATCCTCCTG CTCCTGCAGC AACACCGACC CCCAGGACCC CGCAGCCTAG TGAGGATCCA 
GACGCCCCTC AGGGGGAGAG AGATGGAGAA GGCCAGTGGA CATCTTCCAC ATCAGTAGAG 
CAGGCCAGGG AGCGGGGCTA CCACAGGGCC TTATTGCTGA GTGATCATCG CAAAGATACA 
GATGGTGGAG AGTCAGAAGG TGGTGATTCC CAAGTGAAGG ACTGTTCTTC TCCCAAGAGC 
TGCAAGAGCC CCTCGACACA TGCACCTTGT TCTCCAGCAG CCCAGGCTGT GTCTCGGCCA 
TCAAAGGATG AAGATGAAGC ACAGACTCGT GGTCCTGCTC AGCCTCAGGC AGTGTCCGTC 
CAGCCGTCCA GCGCTAAGAC TCCCAGCTCA AAACCAGCTG CTCTGACGCC CAGCAAGCTC 
CACTGTGGGC CCTCAGGCGC CTCTCAGAGT CACTACGCGG GACCTTCGCT CATGCACTCT 
CCCAAAGCGC AACCCCAAGG ATCACCTTTC CGTGGCCAGC GAGCCTTTCT GACAGCTCAG 
CCTCAGAACC AGCCTCAACC ACAAGCTACA TCTGGCCCTG CAACTTTCCC TCAGTATAAC 
CCTCAAAATG CACCTCCACC TCCGCCCCCT CCTCCCCCAG CTCCACCTAC ATCTGCTCCT 
TACTTCCCCA CCCAGCCTAC TGCTGCTGCT GCTCCCTTTC CTGCATTTAA GCCCACCGTA 
GCCTCGCCCT TTCCTCCTGG TTCCCAGCCT CTACTTCAGA CCCATCATGC ACTGCATTAC 
CAAAGCAGTG CTGCTCCTCC TCCTCCACCA CCTCCTCCCC CTCCCCACCC GCAGCCTGGC 
CCCACCTTAC TGCATGTCAA TCTGCAACCT CCTTCACTGC AGCAGCATCA GCTCCTGCTG 
AGCTCCGCCC CACCTCCGCC TCCTCCTCCA CCTCCTCAGA CACAGAGTTC CCAGCAGCCC 
CCGCCTAACT CCGGCACGCT CCTGTCAATC AAACAAGGAC CACACCCACC CCTTCCACCC 
CCTCCTCCAG CTCCCTCCAC TAACGCACCA CCGCACCCGT TCCAAAACAT CAGTGGCTTT 
CAAACCACTC TACTCCACCA GTCTGCACCA GCCAACCCCT CAGTAACCGC ATCTACTTAT 
CAACAAACTG TATTACCCCC TCCTCCTCCA CCTCCCCCCC AACAAACTCC TCCCACACAG 
ACCCCGCCCA ATCCGAGCAT CCCTCAGATC GCAGGTGGCA ATCGAGGACC CGCTCCTTCC 
TCCGCCTCTT TCCACACCAC CGGATACTTG GGCACAGGAT GGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298714.1
CDS	1084..6051
Translation	MSIVIPVGVDTANTSYLDMAAGSEPESVEASSVVVEKSTYPHQIYSSSSHHSHGYIGLPYADHNYGARPPPTPPASPPPSVLIRPGESLFVSGSRPGENLFVPGGQDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQHIPETYLCERCQPRTLDREHAILLQTRKRENMSDGDTSATESGDEVPLELYTAFQHTPTSITLTTARLGNKQADKKRKKSGEKEPPATARAKKSFREGSRKSSRVKGSAPECEPIDPPSLWENKMKSWMERYEEASSNQYSEEVRLLLRVKEARDGKTLAYNTHTAAFKPPVESQVQKNKRILKAVRDLAADSLIIEYRGKVMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARSFGNEARFIRRSCTPNAEVRHVIEEGMLHLYIYSLRSISKGSEITIGFDYDYGSCKYKVDCACVKGNPECPVLKHNLEPTENLGSSTRRRGRKDKEPSRDESGQNQNLTMDCDGSKGKTLNDAKQRKLSPLRLSISNNQDPELIEDLEEKTSVSNEVEMESEEQIAERRRKMGSPAEESHRHDAGASCCRALRNKETREERKMEAILQAFARMEKREKRREQALERIGTKGEVGGRSDIKEEAPATPEAESPAVIQPLLEVVKEEPGLKPAKVSRNKQRKSFSRNRTHIGQQRRRARTVSTCSDLPPSSPGDTLEPLMAETQDIEMPVAPEADAPPTQPPETSPPHSGSPAPTCRSGQKYPKTKKHLVSEWMGVDKQERVASRTPEPPPERPLRISSDPEVLATQLNSLPGMSCSSHVYSTPKHYVRFSSPFLANRSPSTPGVPTGRRRSREMPETPTSTGSCKKRWLKQALEEEGSTSPGGGRPFLLIPSESPLSPSINGESCSPLPLNGSCSLPELPTPLKKRRLCSLDPCMSETSTPYGSPCATPTRTESTEAPGTPSLLATPPRPRPEEPSTEPSTPLQMPNHPLLQESESSMDSSPDGSRRPSTQDVERPPSLLASPSVRNVSSDTAPQESSKSMGSLSPQPSHTEPQDAAVDEGMEVDAAAAVTSAAGGGGGSEASTSAPETPVSSYPPWMKSPERGGISFSPVNSNLRDLTPSHTLEMGAYRPDSTSSGPFSEAAPFFSCNEESSGVTFTRSLSGDGTGEGSTAKNPQKKKVSLLEYRKRQREARRSGSKGECSSPVSTAPPVDVFPVAVEMAVDPPAPAATPTPRTPQPSEDPDAPQGERDGEGQWTSSTSVEQARERGYHRALLLSDHRKDTDGGESEGGDSQVKDCSSPKSCKSPSTHAPCSPAAQAVSRPSKDEDEAQTRGPAQPQAVSVQPSSAKTPSSKPAALTPSKLHCGPSGASQSHYAGPSLMHSPKAQPQGSPFRGQRAFLTAQPQNQPQPQATSGPATFPQYNPQNAPPPPPPPPPAPPTSAPYFPTQPTAAAAPFPAFKPTVASPFPPGSQPLLQTHHALHYQSSAAPPPPPPPPPPHPQPGPTLLHVNLQPPSLQQHQLLLSSAPPPPPPPPPQTQSSQQPPPNSGTLLSIKQGPHPPLPPPPPAPSTNAPPHPFQNISGFQTTLLHQSAPANPSVTASTYQQTVLPPPPPPPPQQTPPTQTPPNPSIPQIAGGNRGPAPSSASFHTTGYLGTGWH

Target ORF information:

RefSeq Version	XM_009300439.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300439.3

ORF Insert Sequence:

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACGGCCAACA CCTCGTATCT GGACATGGCT 
GCAGGCTCAG AACCAGAATC TGTGGAGGCG AGCTCTGTGG TAGTTGAGAA GTCCACCTAC 
CCTCACCAGA TTTACAGCAG CAGCTCCCAC CACTCCCACG GATACATCGG TCTGCCTTAC 
GCCGACCATA ATTATGGGGC GCGACCCCCA CCCACCCCGC CGGCGTCCCC ACCTCCCTCG 
GTGCTGATCC GGCCGGGCGA GAGCTTGTTT GTGTCCGGAA GCCGGCCTGG TGAGAACTTG 
TTTGTGCCGG GCGGGCAGGA CGAGGCGTCG CGTGGCACCA CGCTCAGCAC CTCGGAGGAC 
GGCAGCTACG GTGCTGACAT CACACGCTGC ATCTGTGGCT TCACCCACGA CGATGGCTAC 
ATGATCTGCT GCGACAAGTG CAGTGTGTGG CAGCACATAG ACTGCATGGG GATCGACAGG 
CAGCACATTC CCGAAACCTA CCTGTGTGAG CGCTGTCAGC CACGCACCCT GGACAGAGAG 
CACGCCATCC TCCTGCAAAC CAGGAAGAGA GAGAACATGT CTGATGGGGA CACCAGTGCT 
ACAGAAAGCG GAGATGAGGT TCCACTAGAG CTGTACACAG CGTTTCAGCA CACCCCTACC 
AGCATCACAC TCACAACAGC ACGTCTGGGT AACAAACAGG CTGATAAGAA ACGAAAGAAG 
AGCGGAGAGA AGGAGCCGCC GGCTACAGCC AGGGCCAAGA AGTCATTCCG TGAAGGCTCT 
AGAAAATCAT CTAGAGTAAA GGGCTCCGCT CCCGAGTGTG AGCCGATTGA TCCTCCCTCT 
CTATGGGAGA ATAAGATGAA GTCGTGGATG GAGCGCTACG AGGAGGCCAG CAGTAACCAG 
TACAGTGAAG AAGTGCGGCT ACTGCTGAGA GTCAAAGAAG CCCGAGATGG AAAAACCCTG 
GCCTACAATA CACACACCGC AGCCTTCAAG CCACCTGTAG AGAGTCAAGT ACAAAAAAAC 
AAGCGAATTC TGAAGGCTGT GCGGGATTTG GCTGCTGATT CATTAATAAT CGAGTACAGG 
GGGAAGGTCA TGCTAAGACA GCAGTTTGAG GCTAATGGCT ACTTCTTTAA GAGGCCATAC 
CCTTTTGTAT TGTTCTACTC TAAGTTTGAC GGTTTGGAAA TGTGCGTAGA CGCACGGAGC 
TTTGGGAATG AGGCCCGATT CATTCGGCGC TCCTGCACCC CTAACGCTGA AGTGCGGCAC 
GTGATTGAAG AGGGCATGCT GCATTTATAC ATTTACTCTC TGAGGTCCAT CTCCAAAGGG 
AGCGAGATCA CCATTGGCTT TGATTACGAC TACGGCAGCT GTAAATATAA GGTGGACTGT 
GCTTGTGTAA AAGGCAATCC GGAGTGTCCT GTGCTCAAGC ATAACCTGGA GCCCACAGAG 
AATCTAGGCT CTAGCACACG ACGGCGAGGC CGTAAGGACA AAGAACCATC GCGGGACGAG 
AGCGGACAAA ATCAGAACCT CACTATGGAC TGTGACGGGT CCAAGGGGAA AACGCTTAAT 
GACGCCAAAC AGAGAAAACT CTCTCCTCTC AGGCTCTCAA TATCTAACAA TCAGGATCCT 
GAGTTAATAG AGGATCTAGA AGAGAAAACC TCCGTTAGCA ATGAAGTAGA GATGGAATCA 
GAGGAGCAGA TTGCAGAAAG GAGGAGGAAG ATGGGCAGCC CGGCAGAGGA GTCCCACCGT 
CACGACGCAG GGGCTTCCTG CTGCAGAGCT CTGAGAAACA AGGAGACACG GGAAGAGAGG 
AAGATGGAGG CCATTCTGCA GGCGTTTGCT CGTATGGAGA AGAGGGAAAA GCGGAGGGAG 
CAAGCGTTGG AGAGGATTGG CACCAAAGGG GAGGTTGGTG GACGCAGCGA TATAAAGGAG 
GAGGCACCTG CAACCCCTGA GGCTGAATCT CCTGCCGTGA TACAGCCGCT GCTAGAGGTA 
GTAAAGGAGG AGCCTGGTCT GAAACCGGCG AAGGTGAGCC GTAACAAACA GAGAAAAAGC 
TTCTCCCGGA ACCGGACGCA CATAGGACAG CAGAGGCGGC GAGCACGAAC TGTCAGCACC 
TGCTCCGACT TGCCCCCCAG TTCCCCTGGT GACACTTTAG AGCCCTTAAT GGCAGAGACT 
CAAGATATAG AGATGCCTGT GGCCCCTGAA GCCGATGCTC CGCCAACGCA ACCCCCTGAA 
ACAAGCCCAC CCCACAGTGG CTCACCAGCC CCAACATGCC GCAGTGGACA GAAGTACCCC 
AAAACTAAAA AGCACTTAGT GAGTGAGTGG ATGGGTGTTG ACAAACAGGA GCGGGTTGCG 
TCACGCACTC CGGAGCCTCC CCCTGAAAGG CCGTTGCGGA TCAGTAGTGA TCCTGAAGTC 
TTAGCCACAC AACTGAACTC TTTACCGGGC ATGTCCTGCA GCTCACACGT CTACAGCACG 
CCAAAACACT ACGTCCGCTT TTCTTCTCCT TTCCTGGCCA ACCGTAGTCC CAGTACTCCT 
GGAGTGCCCA CGGGACGACG GCGCTCGAGA GAGATGCCTG AAACGCCAAC TTCCACTGGC 
TCCTGCAAGA AGCGCTGGCT AAAGCAGGCT TTAGAAGAAG AAGGTTCGAC CAGCCCAGGT 
GGAGGACGAC CTTTTCTGCT CATACCCAGT GAGAGTCCTC TCAGTCCCTC TATAAACGGC 
GAGTCCTGCA GCCCTCTACC CCTCAATGGA AGTTGCTCAT TACCAGAGTT GCCTACACCG 
TTGAAGAAGA GGCGCTTGTG TTCACTCGAT CCCTGCATGT CGGAGACTTC CACCCCATAT 
GGGTCTCCAT GTGCCACGCC AACCCGAACA GAGTCAACAG AAGCACCGGG CACACCTTCG 
CTGCTAGCAA CGCCTCCTCG ACCTCGACCA GAGGAGCCCA GTACCGAGCC CTCGACACCC 
TTGCAGATGC CCAACCACCC TCTGTTACAG GAGAGTGAAT CGTCAATGGA CAGTTCTCCA 
GATGGCAGCC GGAGGCCCAG TACACAAGAT GTTGAGCGAC CTCCTTCGCT GCTGGCATCT 
CCCAGCGTGA GAAATGTGAG CTCTGATACG GCTCCACAGG AGTCTAGTAA ATCTATGGGG 
TCTCTGAGTC CTCAGCCCTC CCACACGGAG CCCCAGGATG CCGCAGTGGA TGAAGGCATG 
GAGGTTGATG CTGCTGCTGC TGTTACTTCT GCTGCTGGTG GTGGTGGTGG TTCAGAAGCA 
TCAACATCAG CTCCTGAAAC GCCAGTCTCC TCTTACCCTC CATGGATGAA GAGTCCAGAG 
CGGGGCGGCA TCTCTTTTTC CCCAGTGAAT TCAAATCTGA GAGACCTCAC TCCTTCACAC 
ACCCTGGAGA TGGGGGCGTA CAGGCCGGAT TCAACCTCCA GTGGCCCTTT TAGTGAAGCC 
GCACCCTTCT TTTCTTGCAA TGAGGAGAGC AGTGGTGTGA CCTTTACCCG CTCGCTAAGT 
GGAGATGGCA CAGGGGAGGG AAGCACTGCA AAAAACCCAC AGAAGAAAAA GGTCTCTCTG 
TTGGAGTACA GAAAGCGTCA GCGTGAGGCA CGGCGGAGCG GCTCCAAAGG CGAGTGTAGC 
TCCCCTGTTT CCACAGCACC ACCAGTAGAC GTGTTCCCTG TGGCGGTGGA AATGGCTGTT 
GATCCTCCTG CTCCTGCAGC AACACCGACC CCCAGGACCC CGCAGCCTAG TGAGGATCCA 
GACGCCCCTC AGGGGGAGAG AGATGGAGAA GGCCAGTGGA CATCTTCCAC ATCAGTAGAG 
CAGGCCAGGG AGCGGGGCTA CCACAGGGCC TTATTGCTGA GTGATCATCG CAAAGATACA 
GATGGTGGAG AGTCAGAAGG TGGTGATTCC CAAGTGAAGG ACTGTTCTTC TCCCAAGAGC 
TGCAAGAGCC CCTCGACACA TGCACCTTGT TCTCCAGCAG CCCAGGCTGT GTCTCGGCCA 
TCAAAGGATG AAGATGAAGC ACAGACTCGT GGTCCTGCTC AGCCTCAGGC AGTGTCCGTC 
CAGCCGTCCA GCGCTAAGAC TCCCAGCTCA AAACCAGCTG CTCTGACGCC CAGCAAGCTC 
CACTGTGGGC CCTCAGGCGC CTCTCAGAGT CACTACGCGG GACCTTCGCT CATGCACTCT 
CCCAAAGCGC AACCCCAAGG ATCACCTTTC CGTGGCCAGC GAGCCTTTCT GACAGCTCAG 
CCTCAGAACC AGCCTCAACC ACAAGCTACA TCTGGCCCTG CAACTTTCCC TCAGTATAAC 
CCTCAAAATG CACCTCCACC TCCGCCCCCT CCTCCCCCAG CTCCACCTAC ATCTGCTCCT 
TACTTCCCCA CCCAGCCTAC TGCTGCTGCT GCTCCCTTTC CTGCATTTAA GCCCACCGTA 
GCCTCGCCCT TTCCTCCTGG TTCCCAGCCT CTACTTCAGA CCCATCATGC ACTGCATTAC 
CAAAGCAGTG CTGCTCCTCC TCCTCCACCA CCTCCTCCCC CTCCCCACCC GCAGCCTGGC 
CCCACCTTAC TGCATGTCAA TCTGCAACCT CCTTCACTGC AGCAGCATCA GCTCCTGCTG 
AGCTCCGCCC CACCTCCGCC TCCTCCTCCA CCTCCTCAGA CACAGAGTTC CCAGCAGCCC 
CCGCCTAACT CCGGCACGCT CCTGTCAATC AAACAAGGAC CACACCCACC CCTTCCACCC 
CCTCCTCCAG CTCCCTCCAC TAACGCACCA CCGCACCCGT TCCAAAACAT CAGTGGCTTT 
CAAACCACTC TACTCCACCA GTCTGCACCA GCCAACCCCT CAGTAACCGC ATCTACTTAT 
CAACAAACTG TATTACCCCC TCCTCCTCCA CCTCCCCCCC AACAAACTCC TCCCACACAG 
ACCCCGCCCA ATCCGAGCAT CCCTCAGATC GCAGGTGGCA ATCGAGGACC CGCTCCTTCC 
TCCGCCTCTT TCCACACCAC CGGATACTTG GGCACAGGAT GGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_021475266.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330941.1 Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X2, mRNA.

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CloneID	ODa39844
Clone ID Related Accession (Same CDS sequence)	XM_009300440.2 , XM_009300439.2 , XM_009300441.2 , XM_009300439.3 , XM_021475266.1
Accession Version	XM_021475266.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	histone-lysine N-methyltransferase 2E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACGGCCAACA CCTCGTATCT GGACATGGCT 
GCAGGCTCAG AACCAGAATC TGTGGAGGCG AGCTCTGTGG TAGTTGAGAA GTCCACCTAC 
CCTCACCAGA TTTACAGCAG CAGCTCCCAC CACTCCCACG GATACATCGG TCTGCCTTAC 
GCCGACCATA ATTATGGGGC GCGACCCCCA CCCACCCCGC CGGCGTCCCC ACCTCCCTCG 
GTGCTGATCC GGCCGGGCGA GAGCTTGTTT GTGTCCGGAA GCCGGCCTGG TGAGAACTTG 
TTTGTGCCGG GCGGGCAGGA CGAGGCGTCG CGTGGCACCA CGCTCAGCAC CTCGGAGGAC 
GGCAGCTACG GTGCTGACAT CACACGCTGC ATCTGTGGCT TCACCCACGA CGATGGCTAC 
ATGATCTGCT GCGACAAGTG CAGTGTGTGG CAGCACATAG ACTGCATGGG GATCGACAGG 
CAGCACATTC CCGAAACCTA CCTGTGTGAG CGCTGTCAGC CACGCACCCT GGACAGAGAG 
CACGCCATCC TCCTGCAAAC CAGGAAGAGA GAGAACATGT CTGATGGGGA CACCAGTGCT 
ACAGAAAGCG GAGATGAGGT TCCACTAGAG CTGTACACAG CGTTTCAGCA CACCCCTACC 
AGCATCACAC TCACAACAGC ACGTCTGGGT AACAAACAGG CTGATAAGAA ACGAAAGAAG 
AGCGGAGAGA AGGAGCCGCC GGCTACAGCC AGGGCCAAGA AGTCATTCCG TGAAGGCTCT 
AGAAAATCAT CTAGAGTAAA GGGCTCCGCT CCCGAGTGTG AGCCGATTGA TCCTCCCTCT 
CTATGGGAGA ATAAGATGAA GTCGTGGATG GAGCGCTACG AGGAGGCCAG CAGTAACCAG 
TACAGTGAAG AAGTGCGGCT ACTGCTGAGA GTCAAAGAAG CCCGAGATGG AAAAACCCTG 
GCCTACAATA CACACACCGC AGCCTTCAAG CCACCTGTAG AGAGTCAAGT ACAAAAAAAC 
AAGCGAATTC TGAAGGCTGT GCGGGATTTG GCTGCTGATT CATTAATAAT CGAGTACAGG 
GGGAAGGTCA TGCTAAGACA GCAGTTTGAG GCTAATGGCT ACTTCTTTAA GAGGCCATAC 
CCTTTTGTAT TGTTCTACTC TAAGTTTGAC GGTTTGGAAA TGTGCGTAGA CGCACGGAGC 
TTTGGGAATG AGGCCCGATT CATTCGGCGC TCCTGCACCC CTAACGCTGA AGTGCGGCAC 
GTGATTGAAG AGGGCATGCT GCATTTATAC ATTTACTCTC TGAGGTCCAT CTCCAAAGGG 
AGCGAGATCA CCATTGGCTT TGATTACGAC TACGGCAGCT GTAAATATAA GGTGGACTGT 
GCTTGTGTAA AAGGCAATCC GGAGTGTCCT GTGCTCAAGC ATAACCTGGA GCCCACAGAG 
AATCTAGGCT CTAGCACACG ACGGCGAGGC CGTAAGGACA AAGAACCATC GCGGGACGAG 
AGCGGACAAA ATCAGAACCT CACTATGGAC TGTGACGGGT CCAAGGGGAA AACGCTTAAT 
GACGCCAAAC AGAGAAAACT CTCTCCTCTC AGGCTCTCAA TATCTAACAA TCAGGATCCT 
GAGTTAATAG AGGATCTAGA AGAGAAAACC TCCGTTAGCA ATGAAGTAGA GATGGAATCA 
GAGGAGCAGA TTGCAGAAAG GAGGAGGAAG ATGGGCAGCC CGGCAGAGGA GTCCCACCGT 
CACGACGCAG GGGCTTCCTG CTGCAGAGCT CTGAGAAACA AGGAGACACG GGAAGAGAGG 
AAGATGGAGG CCATTCTGCA GGCGTTTGCT CGTATGGAGA AGAGGGAAAA GCGGAGGGAG 
CAAGCGTTGG AGAGGATTGG CACCAAAGGG GAGGTTGGTG GACGCAGCGA TATAAAGGAG 
GAGGCACCTG CAACCCCTGA GGCTGAATCT CCTGCCGTGA TACAGCCGCT GCTAGAGGTA 
GTAAAGGAGG AGCCTGGTCT GAAACCGGCG AAGGTGAGCC GTAACAAACA GAGAAAAAGC 
TTCTCCCGGA ACCGGACGCA CATAGGACAG CAGAGGCGGC GAGCACGAAC TGTCAGCACC 
TGCTCCGACT TGCCCCCCAG TTCCCCTGGT GACACTTTAG AGCCCTTAAT GGCAGAGACT 
CAAGATATAG AGATGCCTGT GGCCCCTGAA GCCGATGCTC CGCCAACGCA ACCCCCTGAA 
ACAAGCCCAC CCCACAGTGG CTCACCAGCC CCAACATGCC GCAGTGGACA GAAGTACCCC 
AAAACTAAAA AGCACTTAGT GAGTGAGTGG ATGGGTGTTG ACAAACAGGA GCGGGTTGCG 
TCACGCACTC CGGAGCCTCC CCCTGAAAGG CCGTTGCGGA TCAGTAGTGA TCCTGAAGTC 
TTAGCCACAC AACTGAACTC TTTACCGGGC ATGTCCTGCA GCTCACACGT CTACAGCACG 
CCAAAACACT ACGTCCGCTT TTCTTCTCCT TTCCTGGCCA ACCGTAGTCC CAGTACTCCT 
GGAGTGCCCA CGGGACGACG GCGCTCGAGA GAGATGCCTG AAACGCCAAC TTCCACTGGC 
TCCTGCAAGA AGCGCTGGCT AAAGCAGGCT TTAGAAGAAG AAGGTTCGAC CAGCCCAGGT 
GGAGGACGAC CTTTTCTGCT CATACCCAGT GAGAGTCCTC TCAGTCCCTC TATAAACGGC 
GAGTCCTGCA GCCCTCTACC CCTCAATGGA AGTTGCTCAT TACCAGAGTT GCCTACACCG 
TTGAAGAAGA GGCGCTTGTG TTCACTCGAT CCCTGCATGT CGGAGACTTC CACCCCATAT 
GGGTCTCCAT GTGCCACGCC AACCCGAACA GAGTCAACAG AAGCACCGGG CACACCTTCG 
CTGCTAGCAA CGCCTCCTCG ACCTCGACCA GAGGAGCCCA GTACCGAGCC CTCGACACCC 
TTGCAGATGC CCAACCACCC TCTGTTACAG GAGAGTGAAT CGTCAATGGA CAGTTCTCCA 
GATGGCAGCC GGAGGCCCAG TACACAAGAT GTTGAGCGAC CTCCTTCGCT GCTGGCATCT 
CCCAGCGTGA GAAATGTGAG CTCTGATACG GCTCCACAGG AGTCTAGTAA ATCTATGGGG 
TCTCTGAGTC CTCAGCCCTC CCACACGGAG CCCCAGGATG CCGCAGTGGA TGAAGGCATG 
GAGGTTGATG CTGCTGCTGC TGTTACTTCT GCTGCTGGTG GTGGTGGTGG TTCAGAAGCA 
TCAACATCAG CTCCTGAAAC GCCAGTCTCC TCTTACCCTC CATGGATGAA GAGTCCAGAG 
CGGGGCGGCA TCTCTTTTTC CCCAGTGAAT TCAAATCTGA GAGACCTCAC TCCTTCACAC 
ACCCTGGAGA TGGGGGCGTA CAGGCCGGAT TCAACCTCCA GTGGCCCTTT TAGTGAAGCC 
GCACCCTTCT TTTCTTGCAA TGAGGAGAGC AGTGGTGTGA CCTTTACCCG CTCGCTAAGT 
GGAGATGGCA CAGGGGAGGG AAGCACTGCA AAAAACCCAC AGAAGAAAAA GGTCTCTCTG 
TTGGAGTACA GAAAGCGTCA GCGTGAGGCA CGGCGGAGCG GCTCCAAAGG CGAGTGTAGC 
TCCCCTGTTT CCACAGCACC ACCAGTAGAC GTGTTCCCTG TGGCGGTGGA AATGGCTGTT 
GATCCTCCTG CTCCTGCAGC AACACCGACC CCCAGGACCC CGCAGCCTAG TGAGGATCCA 
GACGCCCCTC AGGGGGAGAG AGATGGAGAA GGCCAGTGGA CATCTTCCAC ATCAGTAGAG 
CAGGCCAGGG AGCGGGGCTA CCACAGGGCC TTATTGCTGA GTGATCATCG CAAAGATACA 
GATGGTGGAG AGTCAGAAGG TGGTGATTCC CAAGTGAAGG ACTGTTCTTC TCCCAAGAGC 
TGCAAGAGCC CCTCGACACA TGCACCTTGT TCTCCAGCAG CCCAGGCTGT GTCTCGGCCA 
TCAAAGGATG AAGATGAAGC ACAGACTCGT GGTCCTGCTC AGCCTCAGGC AGTGTCCGTC 
CAGCCGTCCA GCGCTAAGAC TCCCAGCTCA AAACCAGCTG CTCTGACGCC CAGCAAGCTC 
CACTGTGGGC CCTCAGGCGC CTCTCAGAGT CACTACGCGG GACCTTCGCT CATGCACTCT 
CCCAAAGCGC AACCCCAAGG ATCACCTTTC CGTGGCCAGC GAGCCTTTCT GACAGCTCAG 
CCTCAGAACC AGCCTCAACC ACAAGCTACA TCTGGCCCTG CAACTTTCCC TCAGTATAAC 
CCTCAAAATG CACCTCCACC TCCGCCCCCT CCTCCCCCAG CTCCACCTAC ATCTGCTCCT 
TACTTCCCCA CCCAGCCTAC TGCTGCTGCT GCTCCCTTTC CTGCATTTAA GCCCACCGTA 
GCCTCGCCCT TTCCTCCTGG TTCCCAGCCT CTACTTCAGA CCCATCATGC ACTGCATTAC 
CAAAGCAGTG CTGCTCCTCC TCCTCCACCA CCTCCTCCCC CTCCCCACCC GCAGCCTGGC 
CCCACCTTAC TGCATGTCAA TCTGCAACCT CCTTCACTGC AGCAGCATCA GCTCCTGCTG 
AGCTCCGCCC CACCTCCGCC TCCTCCTCCA CCTCCTCAGA CACAGAGTTC CCAGCAGCCC 
CCGCCTAACT CCGGCACGCT CCTGTCAATC AAACAAGGAC CACACCCACC CCTTCCACCC 
CCTCCTCCAG CTCCCTCCAC TAACGCACCA CCGCACCCGT TCCAAAACAT CAGTGGCTTT 
CAAACCACTC TACTCCACCA GTCTGCACCA GCCAACCCCT CAGTAACCGC ATCTACTTAT 
CAACAAACTG TATTACCCCC TCCTCCTCCA CCTCCCCCCC AACAAACTCC TCCCACACAG 
ACCCCGCCCA ATCCGAGCAT CCCTCAGATC GCAGGTGGCA ATCGAGGACC CGCTCCTTCC 
TCCGCCTCTT TCCACACCAC CGGATACTTG GGCACAGGAT GGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330941.1
CDS	1171..6138
Translation	MSIVIPVGVDTANTSYLDMAAGSEPESVEASSVVVEKSTYPHQIYSSSSHHSHGYIGLPYADHNYGARPPPTPPASPPPSVLIRPGESLFVSGSRPGENLFVPGGQDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQHIPETYLCERCQPRTLDREHAILLQTRKRENMSDGDTSATESGDEVPLELYTAFQHTPTSITLTTARLGNKQADKKRKKSGEKEPPATARAKKSFREGSRKSSRVKGSAPECEPIDPPSLWENKMKSWMERYEEASSNQYSEEVRLLLRVKEARDGKTLAYNTHTAAFKPPVESQVQKNKRILKAVRDLAADSLIIEYRGKVMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARSFGNEARFIRRSCTPNAEVRHVIEEGMLHLYIYSLRSISKGSEITIGFDYDYGSCKYKVDCACVKGNPECPVLKHNLEPTENLGSSTRRRGRKDKEPSRDESGQNQNLTMDCDGSKGKTLNDAKQRKLSPLRLSISNNQDPELIEDLEEKTSVSNEVEMESEEQIAERRRKMGSPAEESHRHDAGASCCRALRNKETREERKMEAILQAFARMEKREKRREQALERIGTKGEVGGRSDIKEEAPATPEAESPAVIQPLLEVVKEEPGLKPAKVSRNKQRKSFSRNRTHIGQQRRRARTVSTCSDLPPSSPGDTLEPLMAETQDIEMPVAPEADAPPTQPPETSPPHSGSPAPTCRSGQKYPKTKKHLVSEWMGVDKQERVASRTPEPPPERPLRISSDPEVLATQLNSLPGMSCSSHVYSTPKHYVRFSSPFLANRSPSTPGVPTGRRRSREMPETPTSTGSCKKRWLKQALEEEGSTSPGGGRPFLLIPSESPLSPSINGESCSPLPLNGSCSLPELPTPLKKRRLCSLDPCMSETSTPYGSPCATPTRTESTEAPGTPSLLATPPRPRPEEPSTEPSTPLQMPNHPLLQESESSMDSSPDGSRRPSTQDVERPPSLLASPSVRNVSSDTAPQESSKSMGSLSPQPSHTEPQDAAVDEGMEVDAAAAVTSAAGGGGGSEASTSAPETPVSSYPPWMKSPERGGISFSPVNSNLRDLTPSHTLEMGAYRPDSTSSGPFSEAAPFFSCNEESSGVTFTRSLSGDGTGEGSTAKNPQKKKVSLLEYRKRQREARRSGSKGECSSPVSTAPPVDVFPVAVEMAVDPPAPAATPTPRTPQPSEDPDAPQGERDGEGQWTSSTSVEQARERGYHRALLLSDHRKDTDGGESEGGDSQVKDCSSPKSCKSPSTHAPCSPAAQAVSRPSKDEDEAQTRGPAQPQAVSVQPSSAKTPSSKPAALTPSKLHCGPSGASQSHYAGPSLMHSPKAQPQGSPFRGQRAFLTAQPQNQPQPQATSGPATFPQYNPQNAPPPPPPPPPAPPTSAPYFPTQPTAAAAPFPAFKPTVASPFPPGSQPLLQTHHALHYQSSAAPPPPPPPPPPHPQPGPTLLHVNLQPPSLQQHQLLLSSAPPPPPPPPPQTQSSQQPPPNSGTLLSIKQGPHPPLPPPPPAPSTNAPPHPFQNISGFQTTLLHQSAPANPSVTASTYQQTVLPPPPPPPPQQTPPTQTPPNPSIPQIAGGNRGPAPSSASFHTTGYLGTGWH

Target ORF information:

RefSeq Version	XM_021475266.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475266.1

ORF Insert Sequence:

ATGAGCATAG TCATCCCAGT AGGGGTGGAC ACGGCCAACA CCTCGTATCT GGACATGGCT 
GCAGGCTCAG AACCAGAATC TGTGGAGGCG AGCTCTGTGG TAGTTGAGAA GTCCACCTAC 
CCTCACCAGA TTTACAGCAG CAGCTCCCAC CACTCCCACG GATACATCGG TCTGCCTTAC 
GCCGACCATA ATTATGGGGC GCGACCCCCA CCCACCCCGC CGGCGTCCCC ACCTCCCTCG 
GTGCTGATCC GGCCGGGCGA GAGCTTGTTT GTGTCCGGAA GCCGGCCTGG TGAGAACTTG 
TTTGTGCCGG GCGGGCAGGA CGAGGCGTCG CGTGGCACCA CGCTCAGCAC CTCGGAGGAC 
GGCAGCTACG GTGCTGACAT CACACGCTGC ATCTGTGGCT TCACCCACGA CGATGGCTAC 
ATGATCTGCT GCGACAAGTG CAGTGTGTGG CAGCACATAG ACTGCATGGG GATCGACAGG 
CAGCACATTC CCGAAACCTA CCTGTGTGAG CGCTGTCAGC CACGCACCCT GGACAGAGAG 
CACGCCATCC TCCTGCAAAC CAGGAAGAGA GAGAACATGT CTGATGGGGA CACCAGTGCT 
ACAGAAAGCG GAGATGAGGT TCCACTAGAG CTGTACACAG CGTTTCAGCA CACCCCTACC 
AGCATCACAC TCACAACAGC ACGTCTGGGT AACAAACAGG CTGATAAGAA ACGAAAGAAG 
AGCGGAGAGA AGGAGCCGCC GGCTACAGCC AGGGCCAAGA AGTCATTCCG TGAAGGCTCT 
AGAAAATCAT CTAGAGTAAA GGGCTCCGCT CCCGAGTGTG AGCCGATTGA TCCTCCCTCT 
CTATGGGAGA ATAAGATGAA GTCGTGGATG GAGCGCTACG AGGAGGCCAG CAGTAACCAG 
TACAGTGAAG AAGTGCGGCT ACTGCTGAGA GTCAAAGAAG CCCGAGATGG AAAAACCCTG 
GCCTACAATA CACACACCGC AGCCTTCAAG CCACCTGTAG AGAGTCAAGT ACAAAAAAAC 
AAGCGAATTC TGAAGGCTGT GCGGGATTTG GCTGCTGATT CATTAATAAT CGAGTACAGG 
GGGAAGGTCA TGCTAAGACA GCAGTTTGAG GCTAATGGCT ACTTCTTTAA GAGGCCATAC 
CCTTTTGTAT TGTTCTACTC TAAGTTTGAC GGTTTGGAAA TGTGCGTAGA CGCACGGAGC 
TTTGGGAATG AGGCCCGATT CATTCGGCGC TCCTGCACCC CTAACGCTGA AGTGCGGCAC 
GTGATTGAAG AGGGCATGCT GCATTTATAC ATTTACTCTC TGAGGTCCAT CTCCAAAGGG 
AGCGAGATCA CCATTGGCTT TGATTACGAC TACGGCAGCT GTAAATATAA GGTGGACTGT 
GCTTGTGTAA AAGGCAATCC GGAGTGTCCT GTGCTCAAGC ATAACCTGGA GCCCACAGAG 
AATCTAGGCT CTAGCACACG ACGGCGAGGC CGTAAGGACA AAGAACCATC GCGGGACGAG 
AGCGGACAAA ATCAGAACCT CACTATGGAC TGTGACGGGT CCAAGGGGAA AACGCTTAAT 
GACGCCAAAC AGAGAAAACT CTCTCCTCTC AGGCTCTCAA TATCTAACAA TCAGGATCCT 
GAGTTAATAG AGGATCTAGA AGAGAAAACC TCCGTTAGCA ATGAAGTAGA GATGGAATCA 
GAGGAGCAGA TTGCAGAAAG GAGGAGGAAG ATGGGCAGCC CGGCAGAGGA GTCCCACCGT 
CACGACGCAG GGGCTTCCTG CTGCAGAGCT CTGAGAAACA AGGAGACACG GGAAGAGAGG 
AAGATGGAGG CCATTCTGCA GGCGTTTGCT CGTATGGAGA AGAGGGAAAA GCGGAGGGAG 
CAAGCGTTGG AGAGGATTGG CACCAAAGGG GAGGTTGGTG GACGCAGCGA TATAAAGGAG 
GAGGCACCTG CAACCCCTGA GGCTGAATCT CCTGCCGTGA TACAGCCGCT GCTAGAGGTA 
GTAAAGGAGG AGCCTGGTCT GAAACCGGCG AAGGTGAGCC GTAACAAACA GAGAAAAAGC 
TTCTCCCGGA ACCGGACGCA CATAGGACAG CAGAGGCGGC GAGCACGAAC TGTCAGCACC 
TGCTCCGACT TGCCCCCCAG TTCCCCTGGT GACACTTTAG AGCCCTTAAT GGCAGAGACT 
CAAGATATAG AGATGCCTGT GGCCCCTGAA GCCGATGCTC CGCCAACGCA ACCCCCTGAA 
ACAAGCCCAC CCCACAGTGG CTCACCAGCC CCAACATGCC GCAGTGGACA GAAGTACCCC 
AAAACTAAAA AGCACTTAGT GAGTGAGTGG ATGGGTGTTG ACAAACAGGA GCGGGTTGCG 
TCACGCACTC CGGAGCCTCC CCCTGAAAGG CCGTTGCGGA TCAGTAGTGA TCCTGAAGTC 
TTAGCCACAC AACTGAACTC TTTACCGGGC ATGTCCTGCA GCTCACACGT CTACAGCACG 
CCAAAACACT ACGTCCGCTT TTCTTCTCCT TTCCTGGCCA ACCGTAGTCC CAGTACTCCT 
GGAGTGCCCA CGGGACGACG GCGCTCGAGA GAGATGCCTG AAACGCCAAC TTCCACTGGC 
TCCTGCAAGA AGCGCTGGCT AAAGCAGGCT TTAGAAGAAG AAGGTTCGAC CAGCCCAGGT 
GGAGGACGAC CTTTTCTGCT CATACCCAGT GAGAGTCCTC TCAGTCCCTC TATAAACGGC 
GAGTCCTGCA GCCCTCTACC CCTCAATGGA AGTTGCTCAT TACCAGAGTT GCCTACACCG 
TTGAAGAAGA GGCGCTTGTG TTCACTCGAT CCCTGCATGT CGGAGACTTC CACCCCATAT 
GGGTCTCCAT GTGCCACGCC AACCCGAACA GAGTCAACAG AAGCACCGGG CACACCTTCG 
CTGCTAGCAA CGCCTCCTCG ACCTCGACCA GAGGAGCCCA GTACCGAGCC CTCGACACCC 
TTGCAGATGC CCAACCACCC TCTGTTACAG GAGAGTGAAT CGTCAATGGA CAGTTCTCCA 
GATGGCAGCC GGAGGCCCAG TACACAAGAT GTTGAGCGAC CTCCTTCGCT GCTGGCATCT 
CCCAGCGTGA GAAATGTGAG CTCTGATACG GCTCCACAGG AGTCTAGTAA ATCTATGGGG 
TCTCTGAGTC CTCAGCCCTC CCACACGGAG CCCCAGGATG CCGCAGTGGA TGAAGGCATG 
GAGGTTGATG CTGCTGCTGC TGTTACTTCT GCTGCTGGTG GTGGTGGTGG TTCAGAAGCA 
TCAACATCAG CTCCTGAAAC GCCAGTCTCC TCTTACCCTC CATGGATGAA GAGTCCAGAG 
CGGGGCGGCA TCTCTTTTTC CCCAGTGAAT TCAAATCTGA GAGACCTCAC TCCTTCACAC 
ACCCTGGAGA TGGGGGCGTA CAGGCCGGAT TCAACCTCCA GTGGCCCTTT TAGTGAAGCC 
GCACCCTTCT TTTCTTGCAA TGAGGAGAGC AGTGGTGTGA CCTTTACCCG CTCGCTAAGT 
GGAGATGGCA CAGGGGAGGG AAGCACTGCA AAAAACCCAC AGAAGAAAAA GGTCTCTCTG 
TTGGAGTACA GAAAGCGTCA GCGTGAGGCA CGGCGGAGCG GCTCCAAAGG CGAGTGTAGC 
TCCCCTGTTT CCACAGCACC ACCAGTAGAC GTGTTCCCTG TGGCGGTGGA AATGGCTGTT 
GATCCTCCTG CTCCTGCAGC AACACCGACC CCCAGGACCC CGCAGCCTAG TGAGGATCCA 
GACGCCCCTC AGGGGGAGAG AGATGGAGAA GGCCAGTGGA CATCTTCCAC ATCAGTAGAG 
CAGGCCAGGG AGCGGGGCTA CCACAGGGCC TTATTGCTGA GTGATCATCG CAAAGATACA 
GATGGTGGAG AGTCAGAAGG TGGTGATTCC CAAGTGAAGG ACTGTTCTTC TCCCAAGAGC 
TGCAAGAGCC CCTCGACACA TGCACCTTGT TCTCCAGCAG CCCAGGCTGT GTCTCGGCCA 
TCAAAGGATG AAGATGAAGC ACAGACTCGT GGTCCTGCTC AGCCTCAGGC AGTGTCCGTC 
CAGCCGTCCA GCGCTAAGAC TCCCAGCTCA AAACCAGCTG CTCTGACGCC CAGCAAGCTC 
CACTGTGGGC CCTCAGGCGC CTCTCAGAGT CACTACGCGG GACCTTCGCT CATGCACTCT 
CCCAAAGCGC AACCCCAAGG ATCACCTTTC CGTGGCCAGC GAGCCTTTCT GACAGCTCAG 
CCTCAGAACC AGCCTCAACC ACAAGCTACA TCTGGCCCTG CAACTTTCCC TCAGTATAAC 
CCTCAAAATG CACCTCCACC TCCGCCCCCT CCTCCCCCAG CTCCACCTAC ATCTGCTCCT 
TACTTCCCCA CCCAGCCTAC TGCTGCTGCT GCTCCCTTTC CTGCATTTAA GCCCACCGTA 
GCCTCGCCCT TTCCTCCTGG TTCCCAGCCT CTACTTCAGA CCCATCATGC ACTGCATTAC 
CAAAGCAGTG CTGCTCCTCC TCCTCCACCA CCTCCTCCCC CTCCCCACCC GCAGCCTGGC 
CCCACCTTAC TGCATGTCAA TCTGCAACCT CCTTCACTGC AGCAGCATCA GCTCCTGCTG 
AGCTCCGCCC CACCTCCGCC TCCTCCTCCA CCTCCTCAGA CACAGAGTTC CCAGCAGCCC 
CCGCCTAACT CCGGCACGCT CCTGTCAATC AAACAAGGAC CACACCCACC CCTTCCACCC 
CCTCCTCCAG CTCCCTCCAC TAACGCACCA CCGCACCCGT TCCAAAACAT CAGTGGCTTT 
CAAACCACTC TACTCCACCA GTCTGCACCA GCCAACCCCT CAGTAACCGC ATCTACTTAT 
CAACAAACTG TATTACCCCC TCCTCCTCCA CCTCCCCCCC AACAAACTCC TCCCACACAG 
ACCCCGCCCA ATCCGAGCAT CCCTCAGATC GCAGGTGGCA ATCGAGGACC CGCTCCTTCC 
TCCGCCTCTT TCCACACCAC CGGATACTTG GGCACAGGAT GGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	kmt2e
Entrez Gene ID	325395
Full Name	lysine (K)-specific methyltransferase 2E
Synonyms	mll5,wu:fc74b02,zgc:64223
General protein information	Preferred Names lysine (K)-specific methyltransferase 2E Names histone-lysine N-methyltransferase MLL5 histone-lysine N-methyltransferase 2E DKEY-105O6.4 myeloid/lymphoid or mixed-lineage leukemia 5 (trithorax homolog, Drosophila)
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Bos taurus (cattle)	MLL5	NP_001075920.2
Mus musculus (house mouse)	Kmt2e	NP_081260.1
Rattus norvegicus (Norway rat)	Kmt2e	NP_001094321.1
Pan troglodytes (chimpanzee)	MLL5	XP_003318779.1
Gallus gallus (chicken)	MLL5	XP_003640389.1
Danio rerio (zebrafish)	kmt2e	XP_692965.5
Xenopus tropicalis (tropical clawed frog)	kmt2e	XP_004913074.1
Homo sapiens (human)	KMT2E	NP_891847.1
	LOC697233	XP_002808408.1
Canis lupus familiaris (dog)	KMT2E	XP_533102.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

kmt2e cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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