als2b cDNA ORF clone, Danio rerio(zebrafish)

The following als2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the als2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa11483 XM_005165740.4
Latest version!
Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa11484 XM_002662296.6
Latest version!
Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa49117 XM_009302175.3
Latest version!
Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa49118 XM_009302176.3
Latest version!
Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa49119 XM_009302177.3
Latest version!
Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
View Old Accession Versions >>
ODa11483 XM_005165740.3 Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa49119 XM_009302177.2 Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa11484 XM_002662296.5 Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa49117 XM_009302175.2 Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa49118 XM_009302176.2 Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa11483
    Clone ID Related Accession (Same CDS sequence) XM_005165740.3 , XM_005165740.4
    Accession Version XM_005165740.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5097bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product alsin isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165740.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
    ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG
    CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC
    AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG
    GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC
    GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG
    TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG
    CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT
    TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA
    ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG
    GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG
    CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA
    GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG
    GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG
    AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC
    AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT
    CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT
    GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG
    CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG
    TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA
    ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC
    CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT
    GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC
    ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA
    GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG
    CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG ACAATGCAGA GTCTGAGGTG
    AAGGCGTCGT CATCCAACAC CACAACTGAA GTATCTCCTC GCCTCCTACG GAGAACAAGT
    CGCCCCCGTA TGCGGGCTGT CCCGCTCGCT TCAGGAGGTA TACCTGAGAC TGATGCCCAC
    CTGCCTTCCT TACAGACGGA AGTGTGGAGC TGGGGACAAG GCCAGGATGG ACAACTTGGA
    CATGGAGACC TCCTTCCCAG ACTGCAGCCT GATTGTGTAA AGAGTCTAAA TGGTAAGGAG
    GTGCTGAAAG TGGCTGCAGG GGCTCATCAC TCTCTCGCTC TGACAGCACA GTCACAGGTT
    TTCTCCTGGG GTTGTAACAC TTCTGGACAG CTGGGACACA TGGAATCGCC CACCACCTTC
    CCTCAATTGA CCAAGCTGTC AGATGGGATT CGTGTGTGGG ATATTGGGGC AGGTCTGCAG
    CACACTCTCT TATTGGCTGA CGGAGACTGT ATTCAGCCAA TCCTGTACTA CAGCGGACAG
    CAGGTGAAGG AAGTGCCGGA GCCAACAGAG GAAGAGGAGC AGACTGCAGA ATACACGCAA
    CAACCTGTGC TGCTGCCCTT CTGTATGAAC TTGGGCTATG TAAGCAGTGT GTATGCAGGT
    GGACGGACGT GTGCAGCGCT GACGGATTGT AATGTCATGG GTTTTATTGC TAGTCTGCAT
    GAGCTGGCGG CTGCAGAGAG AAAATATTAC TGCAAACTCA GCAATATCAA GAGTCTTCTG
    CTCCAGCCAT TGCTCAAGCT GGATTCTCTA AGCTCTGCCC TGGGTCAGTC ATCTGCAGGG
    CTGTTGCAGA ATCTGATTGG TCGAATGAGT CGACTGTCTC AGCTTACAGG TCAAAACTCG
    GCCTCTCTGA CCTCGTTCCT GCAGCGTTCA CGAGACATCC GAGGTCTTGT TATTCTTGAA
    GATGCACATC TGTTCCTGGA CACATATTCA GAGTACTCTT CTGCAGTTGG TGATCTTTTA
    GTCATGGGAG GATTTCAAGC CCTTGTCAAA CCTTGCCATG ATGTGTTTGG GAAAGGAGCA
    GAGGTTGTTC AGAGGTTGTC TGAATGTTCT GAAGAAGGTT TGACCTTGGC AGATGTTCTG
    GCGCATCTCT TTTACCTGCC CATAAAACAC CTGCATGAAT ATGGACGACT GCTGCTTAAA
    CTGGCTACCT GCTATGAGGT GAGTTCTGTG GACTATCAGA AGCTGCAGGA TGCCTGCTCA
    AAGTTCGAAT CCATGGCTCT TCATCTAAAG AGGAGGAGAA AGGAAGCTGA ATACACGCTC
    CACTTCTGGA AGAGTTTTCC TGGAAAGATG ACCGACTCCC TGCGTAAACC GTCTCGTCGG
    CTGATCTGTG AAAGCAGTAA TAAGGCTTTG ACTCTTCAAA ACGCAGGACG TTTTTCCGTC
    AACTGGTTCA TCCTCTTCAA CGACGCACTC GTTCATGCAC AGTTTTCCAC CCATCACATT
    TTTCCATTGG CTACGCTGTG GGTGGAGCCA ATTTCTGAGG AGAGCACAGG CGTATATGGC
    TTAAAAGTGA CTTCGCCTGA GGAAAGCTTC GCTCTGCTGG CTTCATCACC AGCTGAAAAA
    GCAAAGTGGC TTCGTTCTAT AAACCAAGCG GTAGAGCAGG CGCTCAGTGG ATTGGGGCAT
    GATGGGATAC CTCCTTCTAC AGGGATGACG CAGAGAGCAG ATCCACCCAT CTCCCGGACA
    GCATCCTACA CCTTCTACAA AGACAGTCGG CTAAAGGATG CCAAATATGA TGGCCGATGG
    GTCTCAGGGA AACCCCATGG GCGGGGCGTG GTCAAATGGC CAGATGGACG AATGTACACG
    GGGACATTCA AGAACGGTTT TGAAGATGGT TTTGGAGATT ACGTTGTGTC CAATAAAACC
    CTGAACAGCT GTGATCATTA TCAAGGCCAG TGGAAAGATG GCAAGATGCA CGGTTTTGGA
    ACATTCAGAT ATGCCTCTGG TGAAGTGTAC GAAGGCTCAT TTCAAGACAA CATGCGTCAT
    GGTCATGGGA TGCTGCGTAG TGGGAAGCTG AATTCCACCT CTCCCAGTGT CTTTATCGGC
    CAGTGGCAGT ACGACAAAAA GTCTGGCTAT GGAGTTTTTG ATGACATCAC ACGAGGAGAG
    AAGTATATGG GCATGTGGCT GGATGATCAA CGGCAGGGGA ACGGAGTTGT TGTTACACAA
    TTTGGCCTAT ACTATGAAGG TGCATTCAGC AACAACAAAA TGATGGGTAC TGGGGTGTTA
    CTCTCTGAAG ATGACACTAC ATTTGAAGGA GAGTTTTTAG AAGACTGGAC TCTTAATGGA
    AAGGGTGTGT TGACCATGCC GAATGGAGAC TATATTGAAG GCTCTTTTTG TGGAGTTTGG
    GGAACCGGGC TAAAGATGTC AGGTTCCTAC TACAAACCCA GCCTGTACGA CTCAGACAAG
    GAGAAGGGTC ATGCACTTAA ACTGGGCCGG TTAGCAGTTC ACTCTGAAGA GAAGTGGAAG
    GCTGTGTTTT TGGAGTGCTG GAACAGGCTC GGTTGTGATT CCCCTGTACA GGGGCAAACC
    ACGACAGCGT GGGACAACAT TGCCATTGCA CTCACTGCTA ACCTGAGACA GCAGAGAGAC
    AGTCCAGAGT TGCTGAGCCG CTCACATCAC AAAACACTAG AGAGTCTTGA GTGCATCCCT
    CAACATGTGG GTCCTGTAAC ATTAGAGGTG TACCACACGA TCCGTCGGTA CCTGGTGAAG
    GCATGTGATA CTCCTCTGCA TCCTCTGGGT CGGCTGGTGG AGACGCTGGT TTCGGTATAC
    CGGATGACGT ATGTTGGTGT TGGAGCAAAC CGCAGGTTAT TACCGCAGGC CGTCAATGAG
    ATCACGTCCT ATCTCAGCCG TATCTTCCAG CTCGTCAGAT TTCTCTTTCC CGAACTGCCA
    GAAGAAGGAG GTTCACTAGC CGACCCTCCA AAAGAGAAGG TCGGCACAGA TGCTGATGGA
    AAGCTGCTGG AGTCTCCTAA ACCTGGGTGT GTGGTCAGCA GCTCTGCTCT CCTCCTGCCG
    GTGCTGTTGC CACGTCTCTA TCCTCCTCTC TTCACGCTCT ACGCTCTAGA GAAAGAGAAA
    GAGGACGATG TGTACTGGGA GTGTGTGCTG CGACTCAACA AACAGCCAGA TCTGGCACTG
    CTGGCTTTCC TCGGGGTCCA ACAGAAGTTC TGGCCTGTAA CTGTTACAAT ACATGGAGAA
    AAACAACAGG TTCTCTCAAG CACTAAAGAC GCCTGTTTCG CCTCAGCAGT TGAGACTTTG
    CAGCAAATAA GCACAACGTT CACACCCTCA GACAAGCTTC AGGTGATCCA GCTGACCTTC
    GAGGAAATAA CCCAGGAAGT GCTCGCACTC CTGAAACAGG ATTTCTTGTG GTCTATGGAT
    GACCTGTTTC CTGTCTTCCT CTTTGTTGTT CTGCGTGCAC GGATAAGGAA TCTGGGGTCA
    GAGGTTAGTC TCATTGAGGA CCTAATGGAT CCATGTGTAC AACATGGAGA GCATGGCATC
    ATGTTCACCA CCCTCAAGGC GTGCTACTAT CAAATCCAGC ATGAGAAGGT CACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005165797.1
    CDS250..5346
    Translation

    Target ORF information:

    RefSeq Version XM_005165740.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005165740.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
    ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG
    CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC
    AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG
    GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC
    GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG
    TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG
    CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT
    TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA
    ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG
    GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG
    CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA
    GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG
    GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG
    AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC
    AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT
    CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT
    GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG
    CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG
    TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA
    ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC
    CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT
    GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC
    ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA
    GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG
    CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG ACAATGCAGA GTCTGAGGTG
    AAGGCGTCGT CATCCAACAC CACAACTGAA GTATCTCCTC GCCTCCTACG GAGAACAAGT
    CGCCCCCGTA TGCGGGCTGT CCCGCTCGCT TCAGGAGGTA TACCTGAGAC TGATGCCCAC
    CTGCCTTCCT TACAGACGGA AGTGTGGAGC TGGGGACAAG GCCAGGATGG ACAACTTGGA
    CATGGAGACC TCCTTCCCAG ACTGCAGCCT GATTGTGTAA AGAGTCTAAA TGGTAAGGAG
    GTGCTGAAAG TGGCTGCAGG GGCTCATCAC TCTCTCGCTC TGACAGCACA GTCACAGGTT
    TTCTCCTGGG GTTGTAACAC TTCTGGACAG CTGGGACACA TGGAATCGCC CACCACCTTC
    CCTCAATTGA CCAAGCTGTC AGATGGGATT CGTGTGTGGG ATATTGGGGC AGGTCTGCAG
    CACACTCTCT TATTGGCTGA CGGAGACTGT ATTCAGCCAA TCCTGTACTA CAGCGGACAG
    CAGGTGAAGG AAGTGCCGGA GCCAACAGAG GAAGAGGAGC AGACTGCAGA ATACACGCAA
    CAACCTGTGC TGCTGCCCTT CTGTATGAAC TTGGGCTATG TAAGCAGTGT GTATGCAGGT
    GGACGGACGT GTGCAGCGCT GACGGATTGT AATGTCATGG GTTTTATTGC TAGTCTGCAT
    GAGCTGGCGG CTGCAGAGAG AAAATATTAC TGCAAACTCA GCAATATCAA GAGTCTTCTG
    CTCCAGCCAT TGCTCAAGCT GGATTCTCTA AGCTCTGCCC TGGGTCAGTC ATCTGCAGGG
    CTGTTGCAGA ATCTGATTGG TCGAATGAGT CGACTGTCTC AGCTTACAGG TCAAAACTCG
    GCCTCTCTGA CCTCGTTCCT GCAGCGTTCA CGAGACATCC GAGGTCTTGT TATTCTTGAA
    GATGCACATC TGTTCCTGGA CACATATTCA GAGTACTCTT CTGCAGTTGG TGATCTTTTA
    GTCATGGGAG GATTTCAAGC CCTTGTCAAA CCTTGCCATG ATGTGTTTGG GAAAGGAGCA
    GAGGTTGTTC AGAGGTTGTC TGAATGTTCT GAAGAAGGTT TGACCTTGGC AGATGTTCTG
    GCGCATCTCT TTTACCTGCC CATAAAACAC CTGCATGAAT ATGGACGACT GCTGCTTAAA
    CTGGCTACCT GCTATGAGGT GAGTTCTGTG GACTATCAGA AGCTGCAGGA TGCCTGCTCA
    AAGTTCGAAT CCATGGCTCT TCATCTAAAG AGGAGGAGAA AGGAAGCTGA ATACACGCTC
    CACTTCTGGA AGAGTTTTCC TGGAAAGATG ACCGACTCCC TGCGTAAACC GTCTCGTCGG
    CTGATCTGTG AAAGCAGTAA TAAGGCTTTG ACTCTTCAAA ACGCAGGACG TTTTTCCGTC
    AACTGGTTCA TCCTCTTCAA CGACGCACTC GTTCATGCAC AGTTTTCCAC CCATCACATT
    TTTCCATTGG CTACGCTGTG GGTGGAGCCA ATTTCTGAGG AGAGCACAGG CGTATATGGC
    TTAAAAGTGA CTTCGCCTGA GGAAAGCTTC GCTCTGCTGG CTTCATCACC AGCTGAAAAA
    GCAAAGTGGC TTCGTTCTAT AAACCAAGCG GTAGAGCAGG CGCTCAGTGG ATTGGGGCAT
    GATGGGATAC CTCCTTCTAC AGGGATGACG CAGAGAGCAG ATCCACCCAT CTCCCGGACA
    GCATCCTACA CCTTCTACAA AGACAGTCGG CTAAAGGATG CCAAATATGA TGGCCGATGG
    GTCTCAGGGA AACCCCATGG GCGGGGCGTG GTCAAATGGC CAGATGGACG AATGTACACG
    GGGACATTCA AGAACGGTTT TGAAGATGGT TTTGGAGATT ACGTTGTGTC CAATAAAACC
    CTGAACAGCT GTGATCATTA TCAAGGCCAG TGGAAAGATG GCAAGATGCA CGGTTTTGGA
    ACATTCAGAT ATGCCTCTGG TGAAGTGTAC GAAGGCTCAT TTCAAGACAA CATGCGTCAT
    GGTCATGGGA TGCTGCGTAG TGGGAAGCTG AATTCCACCT CTCCCAGTGT CTTTATCGGC
    CAGTGGCAGT ACGACAAAAA GTCTGGCTAT GGAGTTTTTG ATGACATCAC ACGAGGAGAG
    AAGTATATGG GCATGTGGCT GGATGATCAA CGGCAGGGGA ACGGAGTTGT TGTTACACAA
    TTTGGCCTAT ACTATGAAGG TGCATTCAGC AACAACAAAA TGATGGGTAC TGGGGTGTTA
    CTCTCTGAAG ATGACACTAC ATTTGAAGGA GAGTTTTTAG AAGACTGGAC TCTTAATGGA
    AAGGGTGTGT TGACCATGCC GAATGGAGAC TATATTGAAG GCTCTTTTTG TGGAGTTTGG
    GGAACCGGGC TAAAGATGTC AGGTTCCTAC TACAAACCCA GCCTGTACGA CTCAGACAAG
    GAGAAGGGTC ATGCACTTAA ACTGGGCCGG TTAGCAGTTC ACTCTGAAGA GAAGTGGAAG
    GCTGTGTTTT TGGAGTGCTG GAACAGGCTC GGTTGTGATT CCCCTGTACA GGGGCAAACC
    ACGACAGCGT GGGACAACAT TGCCATTGCA CTCACTGCTA ACCTGAGACA GCAGAGAGAC
    AGTCCAGAGT TGCTGAGCCG CTCACATCAC AAAACACTAG AGAGTCTTGA GTGCATCCCT
    CAACATGTGG GTCCTGTAAC ATTAGAGGTG TACCACACGA TCCGTCGGTA CCTGGTGAAG
    GCATGTGATA CTCCTCTGCA TCCTCTGGGT CGGCTGGTGG AGACGCTGGT TTCGGTATAC
    CGGATGACGT ATGTTGGTGT TGGAGCAAAC CGCAGGTTAT TACCGCAGGC CGTCAATGAG
    ATCACGTCCT ATCTCAGCCG TATCTTCCAG CTCGTCAGAT TTCTCTTTCC CGAACTGCCA
    GAAGAAGGAG GTTCACTAGC CGACCCTCCA AAAGAGAAGG TCGGCACAGA TGCTGATGGA
    AAGCTGCTGG AGTCTCCTAA ACCTGGGTGT GTGGTCAGCA GCTCTGCTCT CCTCCTGCCG
    GTGCTGTTGC CACGTCTCTA TCCTCCTCTC TTCACGCTCT ACGCTCTAGA GAAAGAGAAA
    GAGGACGATG TGTACTGGGA GTGTGTGCTG CGACTCAACA AACAGCCAGA TCTGGCACTG
    CTGGCTTTCC TCGGGGTCCA ACAGAAGTTC TGGCCTGTAA CTGTTACAAT ACATGGAGAA
    AAACAACAGG TTCTCTCAAG CACTAAAGAC GCCTGTTTCG CCTCAGCAGT TGAGACTTTG
    CAGCAAATAA GCACAACGTT CACACCCTCA GACAAGCTTC AGGTGATCCA GCTGACCTTC
    GAGGAAATAA CCCAGGAAGT GCTCGCACTC CTGAAACAGG ATTTCTTGTG GTCTATGGAT
    GACCTGTTTC CTGTCTTCCT CTTTGTTGTT CTGCGTGCAC GGATAAGGAA TCTGGGGTCA
    GAGGTTAGTC TCATTGAGGA CCTAATGGAT CCATGTGTAC AACATGGAGA GCATGGCATC
    ATGTTCACCA CCCTCAAGGC GTGCTACTAT CAAATCCAGC ATGAGAAGGT CACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa11483
    Clone ID Related Accession (Same CDS sequence) XM_005165740.3 , XM_005165740.4
    Accession Version XM_005165740.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5097bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product alsin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165740.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
    ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG
    CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC
    AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG
    GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC
    GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG
    TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG
    CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT
    TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA
    ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG
    GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG
    CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA
    GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG
    GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG
    AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC
    AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT
    CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT
    GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG
    CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG
    TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA
    ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC
    CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT
    GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC
    ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA
    GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG
    CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG ACAATGCAGA GTCTGAGGTG
    AAGGCGTCGT CATCCAACAC CACAACTGAA GTATCTCCTC GCCTCCTACG GAGAACAAGT
    CGCCCCCGTA TGCGGGCTGT CCCGCTCGCT TCAGGAGGTA TACCTGAGAC TGATGCCCAC
    CTGCCTTCCT TACAGACGGA AGTGTGGAGC TGGGGACAAG GCCAGGATGG ACAACTTGGA
    CATGGAGACC TCCTTCCCAG ACTGCAGCCT GATTGTGTAA AGAGTCTAAA TGGTAAGGAG
    GTGCTGAAAG TGGCTGCAGG GGCTCATCAC TCTCTCGCTC TGACAGCACA GTCACAGGTT
    TTCTCCTGGG GTTGTAACAC TTCTGGACAG CTGGGACACA TGGAATCGCC CACCACCTTC
    CCTCAATTGA CCAAGCTGTC AGATGGGATT CGTGTGTGGG ATATTGGGGC AGGTCTGCAG
    CACACTCTCT TATTGGCTGA CGGAGACTGT ATTCAGCCAA TCCTGTACTA CAGCGGACAG
    CAGGTGAAGG AAGTGCCGGA GCCAACAGAG GAAGAGGAGC AGACTGCAGA ATACACGCAA
    CAACCTGTGC TGCTGCCCTT CTGTATGAAC TTGGGCTATG TAAGCAGTGT GTATGCAGGT
    GGACGGACGT GTGCAGCGCT GACGGATTGT AATGTCATGG GTTTTATTGC TAGTCTGCAT
    GAGCTGGCGG CTGCAGAGAG AAAATATTAC TGCAAACTCA GCAATATCAA GAGTCTTCTG
    CTCCAGCCAT TGCTCAAGCT GGATTCTCTA AGCTCTGCCC TGGGTCAGTC ATCTGCAGGG
    CTGTTGCAGA ATCTGATTGG TCGAATGAGT CGACTGTCTC AGCTTACAGG TCAAAACTCG
    GCCTCTCTGA CCTCGTTCCT GCAGCGTTCA CGAGACATCC GAGGTCTTGT TATTCTTGAA
    GATGCACATC TGTTCCTGGA CACATATTCA GAGTACTCTT CTGCAGTTGG TGATCTTTTA
    GTCATGGGAG GATTTCAAGC CCTTGTCAAA CCTTGCCATG ATGTGTTTGG GAAAGGAGCA
    GAGGTTGTTC AGAGGTTGTC TGAATGTTCT GAAGAAGGTT TGACCTTGGC AGATGTTCTG
    GCGCATCTCT TTTACCTGCC CATAAAACAC CTGCATGAAT ATGGACGACT GCTGCTTAAA
    CTGGCTACCT GCTATGAGGT GAGTTCTGTG GACTATCAGA AGCTGCAGGA TGCCTGCTCA
    AAGTTCGAAT CCATGGCTCT TCATCTAAAG AGGAGGAGAA AGGAAGCTGA ATACACGCTC
    CACTTCTGGA AGAGTTTTCC TGGAAAGATG ACCGACTCCC TGCGTAAACC GTCTCGTCGG
    CTGATCTGTG AAAGCAGTAA TAAGGCTTTG ACTCTTCAAA ACGCAGGACG TTTTTCCGTC
    AACTGGTTCA TCCTCTTCAA CGACGCACTC GTTCATGCAC AGTTTTCCAC CCATCACATT
    TTTCCATTGG CTACGCTGTG GGTGGAGCCA ATTTCTGAGG AGAGCACAGG CGTATATGGC
    TTAAAAGTGA CTTCGCCTGA GGAAAGCTTC GCTCTGCTGG CTTCATCACC AGCTGAAAAA
    GCAAAGTGGC TTCGTTCTAT AAACCAAGCG GTAGAGCAGG CGCTCAGTGG ATTGGGGCAT
    GATGGGATAC CTCCTTCTAC AGGGATGACG CAGAGAGCAG ATCCACCCAT CTCCCGGACA
    GCATCCTACA CCTTCTACAA AGACAGTCGG CTAAAGGATG CCAAATATGA TGGCCGATGG
    GTCTCAGGGA AACCCCATGG GCGGGGCGTG GTCAAATGGC CAGATGGACG AATGTACACG
    GGGACATTCA AGAACGGTTT TGAAGATGGT TTTGGAGATT ACGTTGTGTC CAATAAAACC
    CTGAACAGCT GTGATCATTA TCAAGGCCAG TGGAAAGATG GCAAGATGCA CGGTTTTGGA
    ACATTCAGAT ATGCCTCTGG TGAAGTGTAC GAAGGCTCAT TTCAAGACAA CATGCGTCAT
    GGTCATGGGA TGCTGCGTAG TGGGAAGCTG AATTCCACCT CTCCCAGTGT CTTTATCGGC
    CAGTGGCAGT ACGACAAAAA GTCTGGCTAT GGAGTTTTTG ATGACATCAC ACGAGGAGAG
    AAGTATATGG GCATGTGGCT GGATGATCAA CGGCAGGGGA ACGGAGTTGT TGTTACACAA
    TTTGGCCTAT ACTATGAAGG TGCATTCAGC AACAACAAAA TGATGGGTAC TGGGGTGTTA
    CTCTCTGAAG ATGACACTAC ATTTGAAGGA GAGTTTTTAG AAGACTGGAC TCTTAATGGA
    AAGGGTGTGT TGACCATGCC GAATGGAGAC TATATTGAAG GCTCTTTTTG TGGAGTTTGG
    GGAACCGGGC TAAAGATGTC AGGTTCCTAC TACAAACCCA GCCTGTACGA CTCAGACAAG
    GAGAAGGGTC ATGCACTTAA ACTGGGCCGG TTAGCAGTTC ACTCTGAAGA GAAGTGGAAG
    GCTGTGTTTT TGGAGTGCTG GAACAGGCTC GGTTGTGATT CCCCTGTACA GGGGCAAACC
    ACGACAGCGT GGGACAACAT TGCCATTGCA CTCACTGCTA ACCTGAGACA GCAGAGAGAC
    AGTCCAGAGT TGCTGAGCCG CTCACATCAC AAAACACTAG AGAGTCTTGA GTGCATCCCT
    CAACATGTGG GTCCTGTAAC ATTAGAGGTG TACCACACGA TCCGTCGGTA CCTGGTGAAG
    GCATGTGATA CTCCTCTGCA TCCTCTGGGT CGGCTGGTGG AGACGCTGGT TTCGGTATAC
    CGGATGACGT ATGTTGGTGT TGGAGCAAAC CGCAGGTTAT TACCGCAGGC CGTCAATGAG
    ATCACGTCCT ATCTCAGCCG TATCTTCCAG CTCGTCAGAT TTCTCTTTCC CGAACTGCCA
    GAAGAAGGAG GTTCACTAGC CGACCCTCCA AAAGAGAAGG TCGGCACAGA TGCTGATGGA
    AAGCTGCTGG AGTCTCCTAA ACCTGGGTGT GTGGTCAGCA GCTCTGCTCT CCTCCTGCCG
    GTGCTGTTGC CACGTCTCTA TCCTCCTCTC TTCACGCTCT ACGCTCTAGA GAAAGAGAAA
    GAGGACGATG TGTACTGGGA GTGTGTGCTG CGACTCAACA AACAGCCAGA TCTGGCACTG
    CTGGCTTTCC TCGGGGTCCA ACAGAAGTTC TGGCCTGTAA CTGTTACAAT ACATGGAGAA
    AAACAACAGG TTCTCTCAAG CACTAAAGAC GCCTGTTTCG CCTCAGCAGT TGAGACTTTG
    CAGCAAATAA GCACAACGTT CACACCCTCA GACAAGCTTC AGGTGATCCA GCTGACCTTC
    GAGGAAATAA CCCAGGAAGT GCTCGCACTC CTGAAACAGG ATTTCTTGTG GTCTATGGAT
    GACCTGTTTC CTGTCTTCCT CTTTGTTGTT CTGCGTGCAC GGATAAGGAA TCTGGGGTCA
    GAGGTTAGTC TCATTGAGGA CCTAATGGAT CCATGTGTAC AACATGGAGA GCATGGCATC
    ATGTTCACCA CCCTCAAGGC GTGCTACTAT CAAATCCAGC ATGAGAAGGT CACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005165797.1
    CDS270..5366
    Translation

    Target ORF information:

    RefSeq Version XM_005165740.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005165740.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
    ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG
    CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC
    AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG
    GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC
    GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG
    TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG
    CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT
    TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA
    ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG
    GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG
    CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA
    GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG
    GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG
    AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC
    AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT
    CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT
    GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG
    CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG
    TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA
    ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC
    CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT
    GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC
    ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA
    GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG
    CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG ACAATGCAGA GTCTGAGGTG
    AAGGCGTCGT CATCCAACAC CACAACTGAA GTATCTCCTC GCCTCCTACG GAGAACAAGT
    CGCCCCCGTA TGCGGGCTGT CCCGCTCGCT TCAGGAGGTA TACCTGAGAC TGATGCCCAC
    CTGCCTTCCT TACAGACGGA AGTGTGGAGC TGGGGACAAG GCCAGGATGG ACAACTTGGA
    CATGGAGACC TCCTTCCCAG ACTGCAGCCT GATTGTGTAA AGAGTCTAAA TGGTAAGGAG
    GTGCTGAAAG TGGCTGCAGG GGCTCATCAC TCTCTCGCTC TGACAGCACA GTCACAGGTT
    TTCTCCTGGG GTTGTAACAC TTCTGGACAG CTGGGACACA TGGAATCGCC CACCACCTTC
    CCTCAATTGA CCAAGCTGTC AGATGGGATT CGTGTGTGGG ATATTGGGGC AGGTCTGCAG
    CACACTCTCT TATTGGCTGA CGGAGACTGT ATTCAGCCAA TCCTGTACTA CAGCGGACAG
    CAGGTGAAGG AAGTGCCGGA GCCAACAGAG GAAGAGGAGC AGACTGCAGA ATACACGCAA
    CAACCTGTGC TGCTGCCCTT CTGTATGAAC TTGGGCTATG TAAGCAGTGT GTATGCAGGT
    GGACGGACGT GTGCAGCGCT GACGGATTGT AATGTCATGG GTTTTATTGC TAGTCTGCAT
    GAGCTGGCGG CTGCAGAGAG AAAATATTAC TGCAAACTCA GCAATATCAA GAGTCTTCTG
    CTCCAGCCAT TGCTCAAGCT GGATTCTCTA AGCTCTGCCC TGGGTCAGTC ATCTGCAGGG
    CTGTTGCAGA ATCTGATTGG TCGAATGAGT CGACTGTCTC AGCTTACAGG TCAAAACTCG
    GCCTCTCTGA CCTCGTTCCT GCAGCGTTCA CGAGACATCC GAGGTCTTGT TATTCTTGAA
    GATGCACATC TGTTCCTGGA CACATATTCA GAGTACTCTT CTGCAGTTGG TGATCTTTTA
    GTCATGGGAG GATTTCAAGC CCTTGTCAAA CCTTGCCATG ATGTGTTTGG GAAAGGAGCA
    GAGGTTGTTC AGAGGTTGTC TGAATGTTCT GAAGAAGGTT TGACCTTGGC AGATGTTCTG
    GCGCATCTCT TTTACCTGCC CATAAAACAC CTGCATGAAT ATGGACGACT GCTGCTTAAA
    CTGGCTACCT GCTATGAGGT GAGTTCTGTG GACTATCAGA AGCTGCAGGA TGCCTGCTCA
    AAGTTCGAAT CCATGGCTCT TCATCTAAAG AGGAGGAGAA AGGAAGCTGA ATACACGCTC
    CACTTCTGGA AGAGTTTTCC TGGAAAGATG ACCGACTCCC TGCGTAAACC GTCTCGTCGG
    CTGATCTGTG AAAGCAGTAA TAAGGCTTTG ACTCTTCAAA ACGCAGGACG TTTTTCCGTC
    AACTGGTTCA TCCTCTTCAA CGACGCACTC GTTCATGCAC AGTTTTCCAC CCATCACATT
    TTTCCATTGG CTACGCTGTG GGTGGAGCCA ATTTCTGAGG AGAGCACAGG CGTATATGGC
    TTAAAAGTGA CTTCGCCTGA GGAAAGCTTC GCTCTGCTGG CTTCATCACC AGCTGAAAAA
    GCAAAGTGGC TTCGTTCTAT AAACCAAGCG GTAGAGCAGG CGCTCAGTGG ATTGGGGCAT
    GATGGGATAC CTCCTTCTAC AGGGATGACG CAGAGAGCAG ATCCACCCAT CTCCCGGACA
    GCATCCTACA CCTTCTACAA AGACAGTCGG CTAAAGGATG CCAAATATGA TGGCCGATGG
    GTCTCAGGGA AACCCCATGG GCGGGGCGTG GTCAAATGGC CAGATGGACG AATGTACACG
    GGGACATTCA AGAACGGTTT TGAAGATGGT TTTGGAGATT ACGTTGTGTC CAATAAAACC
    CTGAACAGCT GTGATCATTA TCAAGGCCAG TGGAAAGATG GCAAGATGCA CGGTTTTGGA
    ACATTCAGAT ATGCCTCTGG TGAAGTGTAC GAAGGCTCAT TTCAAGACAA CATGCGTCAT
    GGTCATGGGA TGCTGCGTAG TGGGAAGCTG AATTCCACCT CTCCCAGTGT CTTTATCGGC
    CAGTGGCAGT ACGACAAAAA GTCTGGCTAT GGAGTTTTTG ATGACATCAC ACGAGGAGAG
    AAGTATATGG GCATGTGGCT GGATGATCAA CGGCAGGGGA ACGGAGTTGT TGTTACACAA
    TTTGGCCTAT ACTATGAAGG TGCATTCAGC AACAACAAAA TGATGGGTAC TGGGGTGTTA
    CTCTCTGAAG ATGACACTAC ATTTGAAGGA GAGTTTTTAG AAGACTGGAC TCTTAATGGA
    AAGGGTGTGT TGACCATGCC GAATGGAGAC TATATTGAAG GCTCTTTTTG TGGAGTTTGG
    GGAACCGGGC TAAAGATGTC AGGTTCCTAC TACAAACCCA GCCTGTACGA CTCAGACAAG
    GAGAAGGGTC ATGCACTTAA ACTGGGCCGG TTAGCAGTTC ACTCTGAAGA GAAGTGGAAG
    GCTGTGTTTT TGGAGTGCTG GAACAGGCTC GGTTGTGATT CCCCTGTACA GGGGCAAACC
    ACGACAGCGT GGGACAACAT TGCCATTGCA CTCACTGCTA ACCTGAGACA GCAGAGAGAC
    AGTCCAGAGT TGCTGAGCCG CTCACATCAC AAAACACTAG AGAGTCTTGA GTGCATCCCT
    CAACATGTGG GTCCTGTAAC ATTAGAGGTG TACCACACGA TCCGTCGGTA CCTGGTGAAG
    GCATGTGATA CTCCTCTGCA TCCTCTGGGT CGGCTGGTGG AGACGCTGGT TTCGGTATAC
    CGGATGACGT ATGTTGGTGT TGGAGCAAAC CGCAGGTTAT TACCGCAGGC CGTCAATGAG
    ATCACGTCCT ATCTCAGCCG TATCTTCCAG CTCGTCAGAT TTCTCTTTCC CGAACTGCCA
    GAAGAAGGAG GTTCACTAGC CGACCCTCCA AAAGAGAAGG TCGGCACAGA TGCTGATGGA
    AAGCTGCTGG AGTCTCCTAA ACCTGGGTGT GTGGTCAGCA GCTCTGCTCT CCTCCTGCCG
    GTGCTGTTGC CACGTCTCTA TCCTCCTCTC TTCACGCTCT ACGCTCTAGA GAAAGAGAAA
    GAGGACGATG TGTACTGGGA GTGTGTGCTG CGACTCAACA AACAGCCAGA TCTGGCACTG
    CTGGCTTTCC TCGGGGTCCA ACAGAAGTTC TGGCCTGTAA CTGTTACAAT ACATGGAGAA
    AAACAACAGG TTCTCTCAAG CACTAAAGAC GCCTGTTTCG CCTCAGCAGT TGAGACTTTG
    CAGCAAATAA GCACAACGTT CACACCCTCA GACAAGCTTC AGGTGATCCA GCTGACCTTC
    GAGGAAATAA CCCAGGAAGT GCTCGCACTC CTGAAACAGG ATTTCTTGTG GTCTATGGAT
    GACCTGTTTC CTGTCTTCCT CTTTGTTGTT CTGCGTGCAC GGATAAGGAA TCTGGGGTCA
    GAGGTTAGTC TCATTGAGGA CCTAATGGAT CCATGTGTAC AACATGGAGA GCATGGCATC
    ATGTTCACCA CCCTCAAGGC GTGCTACTAT CAAATCCAGC ATGAGAAGGT CACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa11484
    Clone ID Related Accession (Same CDS sequence) XM_002662296.5 , XM_002662296.6
    Accession Version XM_002662296.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5046bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product alsin isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002662296.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
    ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG
    CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC
    AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG
    GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC
    GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG
    TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG
    CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT
    TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA
    ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG
    GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG
    CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA
    GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG
    GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG
    AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC
    AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT
    CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT
    GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG
    CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG
    TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA
    ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC
    CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT
    GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC
    ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA
    GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG
    CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG TATCTCCTCG CCTCCTACGG
    AGAACAAGTC GCCCCCGTAT GCGGGCTGTC CCGCTCGCTT CAGGAGGTAT ACCTGAGACT
    GATGCCCACC TGCCTTCCTT ACAGACGGAA GTGTGGAGCT GGGGACAAGG CCAGGATGGA
    CAACTTGGAC ATGGAGACCT CCTTCCCAGA CTGCAGCCTG ATTGTGTAAA GAGTCTAAAT
    GGTAAGGAGG TGCTGAAAGT GGCTGCAGGG GCTCATCACT CTCTCGCTCT GACAGCACAG
    TCACAGGTTT TCTCCTGGGG TTGTAACACT TCTGGACAGC TGGGACACAT GGAATCGCCC
    ACCACCTTCC CTCAATTGAC CAAGCTGTCA GATGGGATTC GTGTGTGGGA TATTGGGGCA
    GGTCTGCAGC ACACTCTCTT ATTGGCTGAC GGAGACTGTA TTCAGCCAAT CCTGTACTAC
    AGCGGACAGC AGGTGAAGGA AGTGCCGGAG CCAACAGAGG AAGAGGAGCA GACTGCAGAA
    TACACGCAAC AACCTGTGCT GCTGCCCTTC TGTATGAACT TGGGCTATGT AAGCAGTGTG
    TATGCAGGTG GACGGACGTG TGCAGCGCTG ACGGATTGTA ATGTCATGGG TTTTATTGCT
    AGTCTGCATG AGCTGGCGGC TGCAGAGAGA AAATATTACT GCAAACTCAG CAATATCAAG
    AGTCTTCTGC TCCAGCCATT GCTCAAGCTG GATTCTCTAA GCTCTGCCCT GGGTCAGTCA
    TCTGCAGGGC TGTTGCAGAA TCTGATTGGT CGAATGAGTC GACTGTCTCA GCTTACAGGT
    CAAAACTCGG CCTCTCTGAC CTCGTTCCTG CAGCGTTCAC GAGACATCCG AGGTCTTGTT
    ATTCTTGAAG ATGCACATCT GTTCCTGGAC ACATATTCAG AGTACTCTTC TGCAGTTGGT
    GATCTTTTAG TCATGGGAGG ATTTCAAGCC CTTGTCAAAC CTTGCCATGA TGTGTTTGGG
    AAAGGAGCAG AGGTTGTTCA GAGGTTGTCT GAATGTTCTG AAGAAGGTTT GACCTTGGCA
    GATGTTCTGG CGCATCTCTT TTACCTGCCC ATAAAACACC TGCATGAATA TGGACGACTG
    CTGCTTAAAC TGGCTACCTG CTATGAGGTG AGTTCTGTGG ACTATCAGAA GCTGCAGGAT
    GCCTGCTCAA AGTTCGAATC CATGGCTCTT CATCTAAAGA GGAGGAGAAA GGAAGCTGAA
    TACACGCTCC ACTTCTGGAA GAGTTTTCCT GGAAAGATGA CCGACTCCCT GCGTAAACCG
    TCTCGTCGGC TGATCTGTGA AAGCAGTAAT AAGGCTTTGA CTCTTCAAAA CGCAGGACGT
    TTTTCCGTCA ACTGGTTCAT CCTCTTCAAC GACGCACTCG TTCATGCACA GTTTTCCACC
    CATCACATTT TTCCATTGGC TACGCTGTGG GTGGAGCCAA TTTCTGAGGA GAGCACAGGC
    GTATATGGCT TAAAAGTGAC TTCGCCTGAG GAAAGCTTCG CTCTGCTGGC TTCATCACCA
    GCTGAAAAAG CAAAGTGGCT TCGTTCTATA AACCAAGCGG TAGAGCAGGC GCTCAGTGGA
    TTGGGGCATG ATGGGATACC TCCTTCTACA GGGATGACGC AGAGAGCAGA TCCACCCATC
    TCCCGGACAG CATCCTACAC CTTCTACAAA GACAGTCGGC TAAAGGATGC CAAATATGAT
    GGCCGATGGG TCTCAGGGAA ACCCCATGGG CGGGGCGTGG TCAAATGGCC AGATGGACGA
    ATGTACACGG GGACATTCAA GAACGGTTTT GAAGATGGTT TTGGAGATTA CGTTGTGTCC
    AATAAAACCC TGAACAGCTG TGATCATTAT CAAGGCCAGT GGAAAGATGG CAAGATGCAC
    GGTTTTGGAA CATTCAGATA TGCCTCTGGT GAAGTGTACG AAGGCTCATT TCAAGACAAC
    ATGCGTCATG GTCATGGGAT GCTGCGTAGT GGGAAGCTGA ATTCCACCTC TCCCAGTGTC
    TTTATCGGCC AGTGGCAGTA CGACAAAAAG TCTGGCTATG GAGTTTTTGA TGACATCACA
    CGAGGAGAGA AGTATATGGG CATGTGGCTG GATGATCAAC GGCAGGGGAA CGGAGTTGTT
    GTTACACAAT TTGGCCTATA CTATGAAGGT GCATTCAGCA ACAACAAAAT GATGGGTACT
    GGGGTGTTAC TCTCTGAAGA TGACACTACA TTTGAAGGAG AGTTTTTAGA AGACTGGACT
    CTTAATGGAA AGGGTGTGTT GACCATGCCG AATGGAGACT ATATTGAAGG CTCTTTTTGT
    GGAGTTTGGG GAACCGGGCT AAAGATGTCA GGTTCCTACT ACAAACCCAG CCTGTACGAC
    TCAGACAAGG AGAAGGGTCA TGCACTTAAA CTGGGCCGGT TAGCAGTTCA CTCTGAAGAG
    AAGTGGAAGG CTGTGTTTTT GGAGTGCTGG AACAGGCTCG GTTGTGATTC CCCTGTACAG
    GGGCAAACCA CGACAGCGTG GGACAACATT GCCATTGCAC TCACTGCTAA CCTGAGACAG
    CAGAGAGACA GTCCAGAGTT GCTGAGCCGC TCACATCACA AAACACTAGA GAGTCTTGAG
    TGCATCCCTC AACATGTGGG TCCTGTAACA TTAGAGGTGT ACCACACGAT CCGTCGGTAC
    CTGGTGAAGG CATGTGATAC TCCTCTGCAT CCTCTGGGTC GGCTGGTGGA GACGCTGGTT
    TCGGTATACC GGATGACGTA TGTTGGTGTT GGAGCAAACC GCAGGTTATT ACCGCAGGCC
    GTCAATGAGA TCACGTCCTA TCTCAGCCGT ATCTTCCAGC TCGTCAGATT TCTCTTTCCC
    GAACTGCCAG AAGAAGGAGG TTCACTAGCC GACCCTCCAA AAGAGAAGGT CGGCACAGAT
    GCTGATGGAA AGCTGCTGGA GTCTCCTAAA CCTGGGTGTG TGGTCAGCAG CTCTGCTCTC
    CTCCTGCCGG TGCTGTTGCC ACGTCTCTAT CCTCCTCTCT TCACGCTCTA CGCTCTAGAG
    AAAGAGAAAG AGGACGATGT GTACTGGGAG TGTGTGCTGC GACTCAACAA ACAGCCAGAT
    CTGGCACTGC TGGCTTTCCT CGGGGTCCAA CAGAAGTTCT GGCCTGTAAC TGTTACAATA
    CATGGAGAAA AACAACAGGT TCTCTCAAGC ACTAAAGACG CCTGTTTCGC CTCAGCAGTT
    GAGACTTTGC AGCAAATAAG CACAACGTTC ACACCCTCAG ACAAGCTTCA GGTGATCCAG
    CTGACCTTCG AGGAAATAAC CCAGGAAGTG CTCGCACTCC TGAAACAGGA TTTCTTGTGG
    TCTATGGATG ACCTGTTTCC TGTCTTCCTC TTTGTTGTTC TGCGTGCACG GATAAGGAAT
    CTGGGGTCAG AGGTTAGTCT CATTGAGGAC CTAATGGATC CATGTGTACA ACATGGAGAG
    CATGGCATCA TGTTCACCAC CCTCAAGGCG TGCTACTATC AAATCCAGCA TGAGAAGGTC
    ACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002662342.2
    CDS250..5295
    Translation

    Target ORF information:

    RefSeq Version XM_002662296.5
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002662296.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
    ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG
    CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC
    AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG
    GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC
    GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG
    TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG
    CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT
    TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA
    ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG
    GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG
    CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA
    GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG
    GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG
    AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC
    AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT
    CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT
    GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG
    CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG
    TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA
    ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC
    CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT
    GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC
    ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA
    GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG
    CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG TATCTCCTCG CCTCCTACGG
    AGAACAAGTC GCCCCCGTAT GCGGGCTGTC CCGCTCGCTT CAGGAGGTAT ACCTGAGACT
    GATGCCCACC TGCCTTCCTT ACAGACGGAA GTGTGGAGCT GGGGACAAGG CCAGGATGGA
    CAACTTGGAC ATGGAGACCT CCTTCCCAGA CTGCAGCCTG ATTGTGTAAA GAGTCTAAAT
    GGTAAGGAGG TGCTGAAAGT GGCTGCAGGG GCTCATCACT CTCTCGCTCT GACAGCACAG
    TCACAGGTTT TCTCCTGGGG TTGTAACACT TCTGGACAGC TGGGACACAT GGAATCGCCC
    ACCACCTTCC CTCAATTGAC CAAGCTGTCA GATGGGATTC GTGTGTGGGA TATTGGGGCA
    GGTCTGCAGC ACACTCTCTT ATTGGCTGAC GGAGACTGTA TTCAGCCAAT CCTGTACTAC
    AGCGGACAGC AGGTGAAGGA AGTGCCGGAG CCAACAGAGG AAGAGGAGCA GACTGCAGAA
    TACACGCAAC AACCTGTGCT GCTGCCCTTC TGTATGAACT TGGGCTATGT AAGCAGTGTG
    TATGCAGGTG GACGGACGTG TGCAGCGCTG ACGGATTGTA ATGTCATGGG TTTTATTGCT
    AGTCTGCATG AGCTGGCGGC TGCAGAGAGA AAATATTACT GCAAACTCAG CAATATCAAG
    AGTCTTCTGC TCCAGCCATT GCTCAAGCTG GATTCTCTAA GCTCTGCCCT GGGTCAGTCA
    TCTGCAGGGC TGTTGCAGAA TCTGATTGGT CGAATGAGTC GACTGTCTCA GCTTACAGGT
    CAAAACTCGG CCTCTCTGAC CTCGTTCCTG CAGCGTTCAC GAGACATCCG AGGTCTTGTT
    ATTCTTGAAG ATGCACATCT GTTCCTGGAC ACATATTCAG AGTACTCTTC TGCAGTTGGT
    GATCTTTTAG TCATGGGAGG ATTTCAAGCC CTTGTCAAAC CTTGCCATGA TGTGTTTGGG
    AAAGGAGCAG AGGTTGTTCA GAGGTTGTCT GAATGTTCTG AAGAAGGTTT GACCTTGGCA
    GATGTTCTGG CGCATCTCTT TTACCTGCCC ATAAAACACC TGCATGAATA TGGACGACTG
    CTGCTTAAAC TGGCTACCTG CTATGAGGTG AGTTCTGTGG ACTATCAGAA GCTGCAGGAT
    GCCTGCTCAA AGTTCGAATC CATGGCTCTT CATCTAAAGA GGAGGAGAAA GGAAGCTGAA
    TACACGCTCC ACTTCTGGAA GAGTTTTCCT GGAAAGATGA CCGACTCCCT GCGTAAACCG
    TCTCGTCGGC TGATCTGTGA AAGCAGTAAT AAGGCTTTGA CTCTTCAAAA CGCAGGACGT
    TTTTCCGTCA ACTGGTTCAT CCTCTTCAAC GACGCACTCG TTCATGCACA GTTTTCCACC
    CATCACATTT TTCCATTGGC TACGCTGTGG GTGGAGCCAA TTTCTGAGGA GAGCACAGGC
    GTATATGGCT TAAAAGTGAC TTCGCCTGAG GAAAGCTTCG CTCTGCTGGC TTCATCACCA
    GCTGAAAAAG CAAAGTGGCT TCGTTCTATA AACCAAGCGG TAGAGCAGGC GCTCAGTGGA
    TTGGGGCATG ATGGGATACC TCCTTCTACA GGGATGACGC AGAGAGCAGA TCCACCCATC
    TCCCGGACAG CATCCTACAC CTTCTACAAA GACAGTCGGC TAAAGGATGC CAAATATGAT
    GGCCGATGGG TCTCAGGGAA ACCCCATGGG CGGGGCGTGG TCAAATGGCC AGATGGACGA
    ATGTACACGG GGACATTCAA GAACGGTTTT GAAGATGGTT TTGGAGATTA CGTTGTGTCC
    AATAAAACCC TGAACAGCTG TGATCATTAT CAAGGCCAGT GGAAAGATGG CAAGATGCAC
    GGTTTTGGAA CATTCAGATA TGCCTCTGGT GAAGTGTACG AAGGCTCATT TCAAGACAAC
    ATGCGTCATG GTCATGGGAT GCTGCGTAGT GGGAAGCTGA ATTCCACCTC TCCCAGTGTC
    TTTATCGGCC AGTGGCAGTA CGACAAAAAG TCTGGCTATG GAGTTTTTGA TGACATCACA
    CGAGGAGAGA AGTATATGGG CATGTGGCTG GATGATCAAC GGCAGGGGAA CGGAGTTGTT
    GTTACACAAT TTGGCCTATA CTATGAAGGT GCATTCAGCA ACAACAAAAT GATGGGTACT
    GGGGTGTTAC TCTCTGAAGA TGACACTACA TTTGAAGGAG AGTTTTTAGA AGACTGGACT
    CTTAATGGAA AGGGTGTGTT GACCATGCCG AATGGAGACT ATATTGAAGG CTCTTTTTGT
    GGAGTTTGGG GAACCGGGCT AAAGATGTCA GGTTCCTACT ACAAACCCAG CCTGTACGAC
    TCAGACAAGG AGAAGGGTCA TGCACTTAAA CTGGGCCGGT TAGCAGTTCA CTCTGAAGAG
    AAGTGGAAGG CTGTGTTTTT GGAGTGCTGG AACAGGCTCG GTTGTGATTC CCCTGTACAG
    GGGCAAACCA CGACAGCGTG GGACAACATT GCCATTGCAC TCACTGCTAA CCTGAGACAG
    CAGAGAGACA GTCCAGAGTT GCTGAGCCGC TCACATCACA AAACACTAGA GAGTCTTGAG
    TGCATCCCTC AACATGTGGG TCCTGTAACA TTAGAGGTGT ACCACACGAT CCGTCGGTAC
    CTGGTGAAGG CATGTGATAC TCCTCTGCAT CCTCTGGGTC GGCTGGTGGA GACGCTGGTT
    TCGGTATACC GGATGACGTA TGTTGGTGTT GGAGCAAACC GCAGGTTATT ACCGCAGGCC
    GTCAATGAGA TCACGTCCTA TCTCAGCCGT ATCTTCCAGC TCGTCAGATT TCTCTTTCCC
    GAACTGCCAG AAGAAGGAGG TTCACTAGCC GACCCTCCAA AAGAGAAGGT CGGCACAGAT
    GCTGATGGAA AGCTGCTGGA GTCTCCTAAA CCTGGGTGTG TGGTCAGCAG CTCTGCTCTC
    CTCCTGCCGG TGCTGTTGCC ACGTCTCTAT CCTCCTCTCT TCACGCTCTA CGCTCTAGAG
    AAAGAGAAAG AGGACGATGT GTACTGGGAG TGTGTGCTGC GACTCAACAA ACAGCCAGAT
    CTGGCACTGC TGGCTTTCCT CGGGGTCCAA CAGAAGTTCT GGCCTGTAAC TGTTACAATA
    CATGGAGAAA AACAACAGGT TCTCTCAAGC ACTAAAGACG CCTGTTTCGC CTCAGCAGTT
    GAGACTTTGC AGCAAATAAG CACAACGTTC ACACCCTCAG ACAAGCTTCA GGTGATCCAG
    CTGACCTTCG AGGAAATAAC CCAGGAAGTG CTCGCACTCC TGAAACAGGA TTTCTTGTGG
    TCTATGGATG ACCTGTTTCC TGTCTTCCTC TTTGTTGTTC TGCGTGCACG GATAAGGAAT
    CTGGGGTCAG AGGTTAGTCT CATTGAGGAC CTAATGGATC CATGTGTACA ACATGGAGAG
    CATGGCATCA TGTTCACCAC CCTCAAGGCG TGCTACTATC AAATCCAGCA TGAGAAGGTC
    ACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa11484
    Clone ID Related Accession (Same CDS sequence) XM_002662296.5 , XM_002662296.6
    Accession Version XM_002662296.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5046bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product alsin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002662296.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
    ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG
    CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC
    AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG
    GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC
    GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG
    TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG
    CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT
    TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA
    ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG
    GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG
    CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA
    GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG
    GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG
    AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC
    AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT
    CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT
    GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG
    CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG
    TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA
    ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC
    CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT
    GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC
    ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA
    GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG
    CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG TATCTCCTCG CCTCCTACGG
    AGAACAAGTC GCCCCCGTAT GCGGGCTGTC CCGCTCGCTT CAGGAGGTAT ACCTGAGACT
    GATGCCCACC TGCCTTCCTT ACAGACGGAA GTGTGGAGCT GGGGACAAGG CCAGGATGGA
    CAACTTGGAC ATGGAGACCT CCTTCCCAGA CTGCAGCCTG ATTGTGTAAA GAGTCTAAAT
    GGTAAGGAGG TGCTGAAAGT GGCTGCAGGG GCTCATCACT CTCTCGCTCT GACAGCACAG
    TCACAGGTTT TCTCCTGGGG TTGTAACACT TCTGGACAGC TGGGACACAT GGAATCGCCC
    ACCACCTTCC CTCAATTGAC CAAGCTGTCA GATGGGATTC GTGTGTGGGA TATTGGGGCA
    GGTCTGCAGC ACACTCTCTT ATTGGCTGAC GGAGACTGTA TTCAGCCAAT CCTGTACTAC
    AGCGGACAGC AGGTGAAGGA AGTGCCGGAG CCAACAGAGG AAGAGGAGCA GACTGCAGAA
    TACACGCAAC AACCTGTGCT GCTGCCCTTC TGTATGAACT TGGGCTATGT AAGCAGTGTG
    TATGCAGGTG GACGGACGTG TGCAGCGCTG ACGGATTGTA ATGTCATGGG TTTTATTGCT
    AGTCTGCATG AGCTGGCGGC TGCAGAGAGA AAATATTACT GCAAACTCAG CAATATCAAG
    AGTCTTCTGC TCCAGCCATT GCTCAAGCTG GATTCTCTAA GCTCTGCCCT GGGTCAGTCA
    TCTGCAGGGC TGTTGCAGAA TCTGATTGGT CGAATGAGTC GACTGTCTCA GCTTACAGGT
    CAAAACTCGG CCTCTCTGAC CTCGTTCCTG CAGCGTTCAC GAGACATCCG AGGTCTTGTT
    ATTCTTGAAG ATGCACATCT GTTCCTGGAC ACATATTCAG AGTACTCTTC TGCAGTTGGT
    GATCTTTTAG TCATGGGAGG ATTTCAAGCC CTTGTCAAAC CTTGCCATGA TGTGTTTGGG
    AAAGGAGCAG AGGTTGTTCA GAGGTTGTCT GAATGTTCTG AAGAAGGTTT GACCTTGGCA
    GATGTTCTGG CGCATCTCTT TTACCTGCCC ATAAAACACC TGCATGAATA TGGACGACTG
    CTGCTTAAAC TGGCTACCTG CTATGAGGTG AGTTCTGTGG ACTATCAGAA GCTGCAGGAT
    GCCTGCTCAA AGTTCGAATC CATGGCTCTT CATCTAAAGA GGAGGAGAAA GGAAGCTGAA
    TACACGCTCC ACTTCTGGAA GAGTTTTCCT GGAAAGATGA CCGACTCCCT GCGTAAACCG
    TCTCGTCGGC TGATCTGTGA AAGCAGTAAT AAGGCTTTGA CTCTTCAAAA CGCAGGACGT
    TTTTCCGTCA ACTGGTTCAT CCTCTTCAAC GACGCACTCG TTCATGCACA GTTTTCCACC
    CATCACATTT TTCCATTGGC TACGCTGTGG GTGGAGCCAA TTTCTGAGGA GAGCACAGGC
    GTATATGGCT TAAAAGTGAC TTCGCCTGAG GAAAGCTTCG CTCTGCTGGC TTCATCACCA
    GCTGAAAAAG CAAAGTGGCT TCGTTCTATA AACCAAGCGG TAGAGCAGGC GCTCAGTGGA
    TTGGGGCATG ATGGGATACC TCCTTCTACA GGGATGACGC AGAGAGCAGA TCCACCCATC
    TCCCGGACAG CATCCTACAC CTTCTACAAA GACAGTCGGC TAAAGGATGC CAAATATGAT
    GGCCGATGGG TCTCAGGGAA ACCCCATGGG CGGGGCGTGG TCAAATGGCC AGATGGACGA
    ATGTACACGG GGACATTCAA GAACGGTTTT GAAGATGGTT TTGGAGATTA CGTTGTGTCC
    AATAAAACCC TGAACAGCTG TGATCATTAT CAAGGCCAGT GGAAAGATGG CAAGATGCAC
    GGTTTTGGAA CATTCAGATA TGCCTCTGGT GAAGTGTACG AAGGCTCATT TCAAGACAAC
    ATGCGTCATG GTCATGGGAT GCTGCGTAGT GGGAAGCTGA ATTCCACCTC TCCCAGTGTC
    TTTATCGGCC AGTGGCAGTA CGACAAAAAG TCTGGCTATG GAGTTTTTGA TGACATCACA
    CGAGGAGAGA AGTATATGGG CATGTGGCTG GATGATCAAC GGCAGGGGAA CGGAGTTGTT
    GTTACACAAT TTGGCCTATA CTATGAAGGT GCATTCAGCA ACAACAAAAT GATGGGTACT
    GGGGTGTTAC TCTCTGAAGA TGACACTACA TTTGAAGGAG AGTTTTTAGA AGACTGGACT
    CTTAATGGAA AGGGTGTGTT GACCATGCCG AATGGAGACT ATATTGAAGG CTCTTTTTGT
    GGAGTTTGGG GAACCGGGCT AAAGATGTCA GGTTCCTACT ACAAACCCAG CCTGTACGAC
    TCAGACAAGG AGAAGGGTCA TGCACTTAAA CTGGGCCGGT TAGCAGTTCA CTCTGAAGAG
    AAGTGGAAGG CTGTGTTTTT GGAGTGCTGG AACAGGCTCG GTTGTGATTC CCCTGTACAG
    GGGCAAACCA CGACAGCGTG GGACAACATT GCCATTGCAC TCACTGCTAA CCTGAGACAG
    CAGAGAGACA GTCCAGAGTT GCTGAGCCGC TCACATCACA AAACACTAGA GAGTCTTGAG
    TGCATCCCTC AACATGTGGG TCCTGTAACA TTAGAGGTGT ACCACACGAT CCGTCGGTAC
    CTGGTGAAGG CATGTGATAC TCCTCTGCAT CCTCTGGGTC GGCTGGTGGA GACGCTGGTT
    TCGGTATACC GGATGACGTA TGTTGGTGTT GGAGCAAACC GCAGGTTATT ACCGCAGGCC
    GTCAATGAGA TCACGTCCTA TCTCAGCCGT ATCTTCCAGC TCGTCAGATT TCTCTTTCCC
    GAACTGCCAG AAGAAGGAGG TTCACTAGCC GACCCTCCAA AAGAGAAGGT CGGCACAGAT
    GCTGATGGAA AGCTGCTGGA GTCTCCTAAA CCTGGGTGTG TGGTCAGCAG CTCTGCTCTC
    CTCCTGCCGG TGCTGTTGCC ACGTCTCTAT CCTCCTCTCT TCACGCTCTA CGCTCTAGAG
    AAAGAGAAAG AGGACGATGT GTACTGGGAG TGTGTGCTGC GACTCAACAA ACAGCCAGAT
    CTGGCACTGC TGGCTTTCCT CGGGGTCCAA CAGAAGTTCT GGCCTGTAAC TGTTACAATA
    CATGGAGAAA AACAACAGGT TCTCTCAAGC ACTAAAGACG CCTGTTTCGC CTCAGCAGTT
    GAGACTTTGC AGCAAATAAG CACAACGTTC ACACCCTCAG ACAAGCTTCA GGTGATCCAG
    CTGACCTTCG AGGAAATAAC CCAGGAAGTG CTCGCACTCC TGAAACAGGA TTTCTTGTGG
    TCTATGGATG ACCTGTTTCC TGTCTTCCTC TTTGTTGTTC TGCGTGCACG GATAAGGAAT
    CTGGGGTCAG AGGTTAGTCT CATTGAGGAC CTAATGGATC CATGTGTACA ACATGGAGAG
    CATGGCATCA TGTTCACCAC CCTCAAGGCG TGCTACTATC AAATCCAGCA TGAGAAGGTC
    ACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002662342.2
    CDS270..5315
    Translation

    Target ORF information:

    RefSeq Version XM_002662296.6
    Organism Danio rerio(zebrafish)
    Definition Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002662296.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
    ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG
    CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC
    AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG
    GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC
    GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG
    TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG
    CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT
    TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA
    ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG
    GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG
    CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA
    GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG
    GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG
    AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC
    AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT
    CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT
    GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG
    CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG
    TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA
    ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC
    CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT
    GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC
    ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA
    GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG
    CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG TATCTCCTCG CCTCCTACGG
    AGAACAAGTC GCCCCCGTAT GCGGGCTGTC CCGCTCGCTT CAGGAGGTAT ACCTGAGACT
    GATGCCCACC TGCCTTCCTT ACAGACGGAA GTGTGGAGCT GGGGACAAGG CCAGGATGGA
    CAACTTGGAC ATGGAGACCT CCTTCCCAGA CTGCAGCCTG ATTGTGTAAA GAGTCTAAAT
    GGTAAGGAGG TGCTGAAAGT GGCTGCAGGG GCTCATCACT CTCTCGCTCT GACAGCACAG
    TCACAGGTTT TCTCCTGGGG TTGTAACACT TCTGGACAGC TGGGACACAT GGAATCGCCC
    ACCACCTTCC CTCAATTGAC CAAGCTGTCA GATGGGATTC GTGTGTGGGA TATTGGGGCA
    GGTCTGCAGC ACACTCTCTT ATTGGCTGAC GGAGACTGTA TTCAGCCAAT CCTGTACTAC
    AGCGGACAGC AGGTGAAGGA AGTGCCGGAG CCAACAGAGG AAGAGGAGCA GACTGCAGAA
    TACACGCAAC AACCTGTGCT GCTGCCCTTC TGTATGAACT TGGGCTATGT AAGCAGTGTG
    TATGCAGGTG GACGGACGTG TGCAGCGCTG ACGGATTGTA ATGTCATGGG TTTTATTGCT
    AGTCTGCATG AGCTGGCGGC TGCAGAGAGA AAATATTACT GCAAACTCAG CAATATCAAG
    AGTCTTCTGC TCCAGCCATT GCTCAAGCTG GATTCTCTAA GCTCTGCCCT GGGTCAGTCA
    TCTGCAGGGC TGTTGCAGAA TCTGATTGGT CGAATGAGTC GACTGTCTCA GCTTACAGGT
    CAAAACTCGG CCTCTCTGAC CTCGTTCCTG CAGCGTTCAC GAGACATCCG AGGTCTTGTT
    ATTCTTGAAG ATGCACATCT GTTCCTGGAC ACATATTCAG AGTACTCTTC TGCAGTTGGT
    GATCTTTTAG TCATGGGAGG ATTTCAAGCC CTTGTCAAAC CTTGCCATGA TGTGTTTGGG
    AAAGGAGCAG AGGTTGTTCA GAGGTTGTCT GAATGTTCTG AAGAAGGTTT GACCTTGGCA
    GATGTTCTGG CGCATCTCTT TTACCTGCCC ATAAAACACC TGCATGAATA TGGACGACTG
    CTGCTTAAAC TGGCTACCTG CTATGAGGTG AGTTCTGTGG ACTATCAGAA GCTGCAGGAT
    GCCTGCTCAA AGTTCGAATC CATGGCTCTT CATCTAAAGA GGAGGAGAAA GGAAGCTGAA
    TACACGCTCC ACTTCTGGAA GAGTTTTCCT GGAAAGATGA CCGACTCCCT GCGTAAACCG
    TCTCGTCGGC TGATCTGTGA AAGCAGTAAT AAGGCTTTGA CTCTTCAAAA CGCAGGACGT
    TTTTCCGTCA ACTGGTTCAT CCTCTTCAAC GACGCACTCG TTCATGCACA GTTTTCCACC
    CATCACATTT TTCCATTGGC TACGCTGTGG GTGGAGCCAA TTTCTGAGGA GAGCACAGGC
    GTATATGGCT TAAAAGTGAC TTCGCCTGAG GAAAGCTTCG CTCTGCTGGC TTCATCACCA
    GCTGAAAAAG CAAAGTGGCT TCGTTCTATA AACCAAGCGG TAGAGCAGGC GCTCAGTGGA
    TTGGGGCATG ATGGGATACC TCCTTCTACA GGGATGACGC AGAGAGCAGA TCCACCCATC
    TCCCGGACAG CATCCTACAC CTTCTACAAA GACAGTCGGC TAAAGGATGC CAAATATGAT
    GGCCGATGGG TCTCAGGGAA ACCCCATGGG CGGGGCGTGG TCAAATGGCC AGATGGACGA
    ATGTACACGG GGACATTCAA GAACGGTTTT GAAGATGGTT TTGGAGATTA CGTTGTGTCC
    AATAAAACCC TGAACAGCTG TGATCATTAT CAAGGCCAGT GGAAAGATGG CAAGATGCAC
    GGTTTTGGAA CATTCAGATA TGCCTCTGGT GAAGTGTACG AAGGCTCATT TCAAGACAAC
    ATGCGTCATG GTCATGGGAT GCTGCGTAGT GGGAAGCTGA ATTCCACCTC TCCCAGTGTC
    TTTATCGGCC AGTGGCAGTA CGACAAAAAG TCTGGCTATG GAGTTTTTGA TGACATCACA
    CGAGGAGAGA AGTATATGGG CATGTGGCTG GATGATCAAC GGCAGGGGAA CGGAGTTGTT
    GTTACACAAT TTGGCCTATA CTATGAAGGT GCATTCAGCA ACAACAAAAT GATGGGTACT
    GGGGTGTTAC TCTCTGAAGA TGACACTACA TTTGAAGGAG AGTTTTTAGA AGACTGGACT
    CTTAATGGAA AGGGTGTGTT GACCATGCCG AATGGAGACT ATATTGAAGG CTCTTTTTGT
    GGAGTTTGGG GAACCGGGCT AAAGATGTCA GGTTCCTACT ACAAACCCAG CCTGTACGAC
    TCAGACAAGG AGAAGGGTCA TGCACTTAAA CTGGGCCGGT TAGCAGTTCA CTCTGAAGAG
    AAGTGGAAGG CTGTGTTTTT GGAGTGCTGG AACAGGCTCG GTTGTGATTC CCCTGTACAG
    GGGCAAACCA CGACAGCGTG GGACAACATT GCCATTGCAC TCACTGCTAA CCTGAGACAG
    CAGAGAGACA GTCCAGAGTT GCTGAGCCGC TCACATCACA AAACACTAGA GAGTCTTGAG
    TGCATCCCTC AACATGTGGG TCCTGTAACA TTAGAGGTGT ACCACACGAT CCGTCGGTAC
    CTGGTGAAGG CATGTGATAC TCCTCTGCAT CCTCTGGGTC GGCTGGTGGA GACGCTGGTT
    TCGGTATACC GGATGACGTA TGTTGGTGTT GGAGCAAACC GCAGGTTATT ACCGCAGGCC
    GTCAATGAGA TCACGTCCTA TCTCAGCCGT ATCTTCCAGC TCGTCAGATT TCTCTTTCCC
    GAACTGCCAG AAGAAGGAGG TTCACTAGCC GACCCTCCAA AAGAGAAGGT CGGCACAGAT
    GCTGATGGAA AGCTGCTGGA GTCTCCTAAA CCTGGGTGTG TGGTCAGCAG CTCTGCTCTC
    CTCCTGCCGG TGCTGTTGCC ACGTCTCTAT CCTCCTCTCT TCACGCTCTA CGCTCTAGAG
    AAAGAGAAAG AGGACGATGT GTACTGGGAG TGTGTGCTGC GACTCAACAA ACAGCCAGAT
    CTGGCACTGC TGGCTTTCCT CGGGGTCCAA CAGAAGTTCT GGCCTGTAAC TGTTACAATA
    CATGGAGAAA AACAACAGGT TCTCTCAAGC ACTAAAGACG CCTGTTTCGC CTCAGCAGTT
    GAGACTTTGC AGCAAATAAG CACAACGTTC ACACCCTCAG ACAAGCTTCA GGTGATCCAG
    CTGACCTTCG AGGAAATAAC CCAGGAAGTG CTCGCACTCC TGAAACAGGA TTTCTTGTGG
    TCTATGGATG ACCTGTTTCC TGTCTTCCTC TTTGTTGTTC TGCGTGCACG GATAAGGAAT
    CTGGGGTCAG AGGTTAGTCT CATTGAGGAC CTAATGGATC CATGTGTACA ACATGGAGAG
    CATGGCATCA TGTTCACCAC CCTCAAGGCG TGCTACTATC AAATCCAGCA TGAGAAGGTC
    ACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49117
    Clone ID Related Accession (Same CDS sequence) XM_009302175.3 , XM_009302175.2
    Accession Version XM_009302175.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5121bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product alsin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009302175.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
    ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG
    CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC
    AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG
    GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC
    GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG
    TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG
    CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT
    TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA
    ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG
    GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG
    CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA
    GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG
    GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG
    AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC
    AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT
    CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT
    GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG
    CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG
    TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA
    ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC
    CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT
    GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC
    ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA
    GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG
    CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG ACAATGCAGA GTCTGAGGTG
    AAGGCGTCGT CATCCAACAC CACAACTGAA GTATCTCCTC GCCTCCTACG GAGAACAAGT
    CGCCCCCGTA TGCGGGCTGT CCCGCTCGCT TCAGGAGGTA TACCTGAGAC TGATGCCCAC
    CTGCCTTCCT TACAGACGGA AGTGTGGAGC TGGGGACAAG GCCAGGATGG ACAACTTGGA
    CATGGAGACC TCCTTCCCAG ACTGCAGCCT GATTGTGTAA AGAGTCTAAA TGGTAAGGAG
    GTGCTGAAAG TGGCTGCAGG GGCTCATCAC TCTCTCGCTC TGACAGCACA GTCACAGGTT
    TTCTCCTGGG GTTGTAACAC TTCTGGACAG CTGGGACACA TGGAATCGCC CACCACCTTC
    CCTCAATTGA CCAAGCTGTC AGATGGGATT CGTGTGTGGG ATATTGGGGC AGGTCTGCAG
    CACACTCTCT TATTGGCTGA CGGAGACTGT ATTCAGCCAA TCCTGTACTA CAGCGGACAG
    CAGGTGAAGG AAGTGCCGGA GCCAACAGAG GAAGAGGAGC AGACTGCAGA ATACACGCAA
    CAACCTGTGC TGCTGCCCTT CTGTATGAAC TTGGGCTATG TAAGCAGTGT GTATGCAGGT
    GGACGGACGT GTGCAGCGCT GACGGATTGT AATGTCATGG GTTTTATTGC TAGTCTGCAT
    GAGCTGGCGG CTGCAGAGAG AAAATATTAC TGCAAACTCA GCAATATCAA GAGTCTTCTG
    CTCCAGCCAT TGCTCAAGCT GGATTCTCTA AGCTCTGCCC TGGGTCAGTC ATCTGCAGGG
    CTGTTGCAGA ATCTGATTGG TCGAATGAGT CGACTGTCTC AGCTTACAGG TCAAAACTCG
    GCCTCTCTGA CCTCGTTCCT GCAGCGTTCA CGAGACATCC GAGGTCTTGT TATTCTTGAA
    GATGCACATC TGTTCCTGGA CACATATTCA GAGTACTCTT CTGCAGTTGG TGATCTTTTA
    GTCATGGGAG GATTTCAAGC CCTTGTCAAA CCTTGCCATG ATGTGTTTGG GAAAGGAGCA
    GAGGTTGTTC AGAGGTTGTC TGAATGTTCT GAAGAAGGTT TGACCTTGGC AGATGTTCTG
    GCGCATCTCT TTTACCTGCC CATAAAACAC CTGCATGAAT ATGGACGACT GCTGCTTAAA
    CTGGCTACCT GCTATGAGGT GAGTTCTGTG GACTATCAGA AGCTGCAGGA TGCCTGCTCA
    AAGTTCGAAT CCATGGCTCT TCATCTAAAG AGGAGGAGAA AGGAAGCTGA ATACACGCTC
    CACTTCTGGA AGAGTTTTCC TGGAAAGATG ACCGACTCCC TGCGTAAACC GTCTCGTCGG
    CTGATCTGTG AAAGCAGTAA TAAGGCTTTG ACTCTTCAAA ACGCAGGACG TTTTTCCGTC
    AACTGGTTCA TCCTCTTCAA CGACGCACTC GTTCATGCAC AGGGTATGGT CCCCTATAAG
    AGTCTTTTTT CCACCCATCA CATTTTTCCA TTGGCTACGC TGTGGGTGGA GCCAATTTCT
    GAGGAGAGCA CAGGCGTATA TGGCTTAAAA GTGACTTCGC CTGAGGAAAG CTTCGCTCTG
    CTGGCTTCAT CACCAGCTGA AAAAGCAAAG TGGCTTCGTT CTATAAACCA AGCGGTAGAG
    CAGGCGCTCA GTGGATTGGG GCATGATGGG ATACCTCCTT CTACAGGGAT GACGCAGAGA
    GCAGATCCAC CCATCTCCCG GACAGCATCC TACACCTTCT ACAAAGACAG TCGGCTAAAG
    GATGCCAAAT ATGATGGCCG ATGGGTCTCA GGGAAACCCC ATGGGCGGGG CGTGGTCAAA
    TGGCCAGATG GACGAATGTA CACGGGGACA TTCAAGAACG GTTTTGAAGA TGGTTTTGGA
    GATTACGTTG TGTCCAATAA AACCCTGAAC AGCTGTGATC ATTATCAAGG CCAGTGGAAA
    GATGGCAAGA TGCACGGTTT TGGAACATTC AGATATGCCT CTGGTGAAGT GTACGAAGGC
    TCATTTCAAG ACAACATGCG TCATGGTCAT GGGATGCTGC GTAGTGGGAA GCTGAATTCC
    ACCTCTCCCA GTGTCTTTAT CGGCCAGTGG CAGTACGACA AAAAGTCTGG CTATGGAGTT
    TTTGATGACA TCACACGAGG AGAGAAGTAT ATGGGCATGT GGCTGGATGA TCAACGGCAG
    GGGAACGGAG TTGTTGTTAC ACAATTTGGC CTATACTATG AAGGTGCATT CAGCAACAAC
    AAAATGATGG GTACTGGGGT GTTACTCTCT GAAGATGACA CTACATTTGA AGGAGAGTTT
    TTAGAAGACT GGACTCTTAA TGGAAAGGGT GTGTTGACCA TGCCGAATGG AGACTATATT
    GAAGGCTCTT TTTGTGGAGT TTGGGGAACC GGGCTAAAGA TGTCAGGTTC CTACTACAAA
    CCCAGCCTGT ACGACTCAGA CAAGGAGAAG GGTCATGCAC TTAAACTGGG CCGGTTAGCA
    GTTCACTCTG AAGAGAAGTG GAAGGCTGTG TTTTTGGAGT GCTGGAACAG GCTCGGTTGT
    GATTCCCCTG TACAGGGGCA AACCACGACA GCGTGGGACA ACATTGCCAT TGCACTCACT
    GCTAACCTGA GACAGCAGAG AGACAGTCCA GAGTTGCTGA GCCGCTCACA TCACAAAACA
    CTAGAGAGTC TTGAGTGCAT CCCTCAACAT GTGGGTCCTG TAACATTAGA GGTGTACCAC
    ACGATCCGTC GGTACCTGGT GAAGGCATGT GATACTCCTC TGCATCCTCT GGGTCGGCTG
    GTGGAGACGC TGGTTTCGGT ATACCGGATG ACGTATGTTG GTGTTGGAGC AAACCGCAGG
    TTATTACCGC AGGCCGTCAA TGAGATCACG TCCTATCTCA GCCGTATCTT CCAGCTCGTC
    AGATTTCTCT TTCCCGAACT GCCAGAAGAA GGAGGTTCAC TAGCCGACCC TCCAAAAGAG
    AAGGTCGGCA CAGATGCTGA TGGAAAGCTG CTGGAGTCTC CTAAACCTGG GTGTGTGGTC
    AGCAGCTCTG CTCTCCTCCT GCCGGTGCTG TTGCCACGTC TCTATCCTCC TCTCTTCACG
    CTCTACGCTC TAGAGAAAGA GAAAGAGGAC GATGTGTACT GGGAGTGTGT GCTGCGACTC
    AACAAACAGC CAGATCTGGC ACTGCTGGCT TTCCTCGGGG TCCAACAGAA GTTCTGGCCT
    GTAACTGTTA CAATACATGG AGAAAAACAA CAGGTTCTCT CAAGCACTAA AGACGCCTGT
    TTCGCCTCAG CAGTTGAGAC TTTGCAGCAA ATAAGCACAA CGTTCACACC CTCAGACAAG
    CTTCAGGTGA TCCAGCTGAC CTTCGAGGAA ATAACCCAGG AAGTGCTCGC ACTCCTGAAA
    CAGGATTTCT TGTGGTCTAT GGATGACCTG TTTCCTGTCT TCCTCTTTGT TGTTCTGCGT
    GCACGGATAA GGAATCTGGG GTCAGAGGTT AGTCTCATTG AGGACCTAAT GGATCCATGT
    GTACAACATG GAGAGCATGG CATCATGTTC ACCACCCTCA AGGCGTGCTA CTATCAAATC
    CAGCATGAGA AGGTCACGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009300450.1
    CDS270..5390
    Translation

    Target ORF information:

    RefSeq Version XM_009302175.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009302175.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
    ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG
    CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC
    AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG
    GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC
    GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG
    TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG
    CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT
    TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA
    ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG
    GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG
    CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA
    GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG
    GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG
    AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC
    AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT
    CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT
    GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG
    CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG
    TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA
    ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC
    CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT
    GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC
    ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA
    GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG
    CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG ACAATGCAGA GTCTGAGGTG
    AAGGCGTCGT CATCCAACAC CACAACTGAA GTATCTCCTC GCCTCCTACG GAGAACAAGT
    CGCCCCCGTA TGCGGGCTGT CCCGCTCGCT TCAGGAGGTA TACCTGAGAC TGATGCCCAC
    CTGCCTTCCT TACAGACGGA AGTGTGGAGC TGGGGACAAG GCCAGGATGG ACAACTTGGA
    CATGGAGACC TCCTTCCCAG ACTGCAGCCT GATTGTGTAA AGAGTCTAAA TGGTAAGGAG
    GTGCTGAAAG TGGCTGCAGG GGCTCATCAC TCTCTCGCTC TGACAGCACA GTCACAGGTT
    TTCTCCTGGG GTTGTAACAC TTCTGGACAG CTGGGACACA TGGAATCGCC CACCACCTTC
    CCTCAATTGA CCAAGCTGTC AGATGGGATT CGTGTGTGGG ATATTGGGGC AGGTCTGCAG
    CACACTCTCT TATTGGCTGA CGGAGACTGT ATTCAGCCAA TCCTGTACTA CAGCGGACAG
    CAGGTGAAGG AAGTGCCGGA GCCAACAGAG GAAGAGGAGC AGACTGCAGA ATACACGCAA
    CAACCTGTGC TGCTGCCCTT CTGTATGAAC TTGGGCTATG TAAGCAGTGT GTATGCAGGT
    GGACGGACGT GTGCAGCGCT GACGGATTGT AATGTCATGG GTTTTATTGC TAGTCTGCAT
    GAGCTGGCGG CTGCAGAGAG AAAATATTAC TGCAAACTCA GCAATATCAA GAGTCTTCTG
    CTCCAGCCAT TGCTCAAGCT GGATTCTCTA AGCTCTGCCC TGGGTCAGTC ATCTGCAGGG
    CTGTTGCAGA ATCTGATTGG TCGAATGAGT CGACTGTCTC AGCTTACAGG TCAAAACTCG
    GCCTCTCTGA CCTCGTTCCT GCAGCGTTCA CGAGACATCC GAGGTCTTGT TATTCTTGAA
    GATGCACATC TGTTCCTGGA CACATATTCA GAGTACTCTT CTGCAGTTGG TGATCTTTTA
    GTCATGGGAG GATTTCAAGC CCTTGTCAAA CCTTGCCATG ATGTGTTTGG GAAAGGAGCA
    GAGGTTGTTC AGAGGTTGTC TGAATGTTCT GAAGAAGGTT TGACCTTGGC AGATGTTCTG
    GCGCATCTCT TTTACCTGCC CATAAAACAC CTGCATGAAT ATGGACGACT GCTGCTTAAA
    CTGGCTACCT GCTATGAGGT GAGTTCTGTG GACTATCAGA AGCTGCAGGA TGCCTGCTCA
    AAGTTCGAAT CCATGGCTCT TCATCTAAAG AGGAGGAGAA AGGAAGCTGA ATACACGCTC
    CACTTCTGGA AGAGTTTTCC TGGAAAGATG ACCGACTCCC TGCGTAAACC GTCTCGTCGG
    CTGATCTGTG AAAGCAGTAA TAAGGCTTTG ACTCTTCAAA ACGCAGGACG TTTTTCCGTC
    AACTGGTTCA TCCTCTTCAA CGACGCACTC GTTCATGCAC AGGGTATGGT CCCCTATAAG
    AGTCTTTTTT CCACCCATCA CATTTTTCCA TTGGCTACGC TGTGGGTGGA GCCAATTTCT
    GAGGAGAGCA CAGGCGTATA TGGCTTAAAA GTGACTTCGC CTGAGGAAAG CTTCGCTCTG
    CTGGCTTCAT CACCAGCTGA AAAAGCAAAG TGGCTTCGTT CTATAAACCA AGCGGTAGAG
    CAGGCGCTCA GTGGATTGGG GCATGATGGG ATACCTCCTT CTACAGGGAT GACGCAGAGA
    GCAGATCCAC CCATCTCCCG GACAGCATCC TACACCTTCT ACAAAGACAG TCGGCTAAAG
    GATGCCAAAT ATGATGGCCG ATGGGTCTCA GGGAAACCCC ATGGGCGGGG CGTGGTCAAA
    TGGCCAGATG GACGAATGTA CACGGGGACA TTCAAGAACG GTTTTGAAGA TGGTTTTGGA
    GATTACGTTG TGTCCAATAA AACCCTGAAC AGCTGTGATC ATTATCAAGG CCAGTGGAAA
    GATGGCAAGA TGCACGGTTT TGGAACATTC AGATATGCCT CTGGTGAAGT GTACGAAGGC
    TCATTTCAAG ACAACATGCG TCATGGTCAT GGGATGCTGC GTAGTGGGAA GCTGAATTCC
    ACCTCTCCCA GTGTCTTTAT CGGCCAGTGG CAGTACGACA AAAAGTCTGG CTATGGAGTT
    TTTGATGACA TCACACGAGG AGAGAAGTAT ATGGGCATGT GGCTGGATGA TCAACGGCAG
    GGGAACGGAG TTGTTGTTAC ACAATTTGGC CTATACTATG AAGGTGCATT CAGCAACAAC
    AAAATGATGG GTACTGGGGT GTTACTCTCT GAAGATGACA CTACATTTGA AGGAGAGTTT
    TTAGAAGACT GGACTCTTAA TGGAAAGGGT GTGTTGACCA TGCCGAATGG AGACTATATT
    GAAGGCTCTT TTTGTGGAGT TTGGGGAACC GGGCTAAAGA TGTCAGGTTC CTACTACAAA
    CCCAGCCTGT ACGACTCAGA CAAGGAGAAG GGTCATGCAC TTAAACTGGG CCGGTTAGCA
    GTTCACTCTG AAGAGAAGTG GAAGGCTGTG TTTTTGGAGT GCTGGAACAG GCTCGGTTGT
    GATTCCCCTG TACAGGGGCA AACCACGACA GCGTGGGACA ACATTGCCAT TGCACTCACT
    GCTAACCTGA GACAGCAGAG AGACAGTCCA GAGTTGCTGA GCCGCTCACA TCACAAAACA
    CTAGAGAGTC TTGAGTGCAT CCCTCAACAT GTGGGTCCTG TAACATTAGA GGTGTACCAC
    ACGATCCGTC GGTACCTGGT GAAGGCATGT GATACTCCTC TGCATCCTCT GGGTCGGCTG
    GTGGAGACGC TGGTTTCGGT ATACCGGATG ACGTATGTTG GTGTTGGAGC AAACCGCAGG
    TTATTACCGC AGGCCGTCAA TGAGATCACG TCCTATCTCA GCCGTATCTT CCAGCTCGTC
    AGATTTCTCT TTCCCGAACT GCCAGAAGAA GGAGGTTCAC TAGCCGACCC TCCAAAAGAG
    AAGGTCGGCA CAGATGCTGA TGGAAAGCTG CTGGAGTCTC CTAAACCTGG GTGTGTGGTC
    AGCAGCTCTG CTCTCCTCCT GCCGGTGCTG TTGCCACGTC TCTATCCTCC TCTCTTCACG
    CTCTACGCTC TAGAGAAAGA GAAAGAGGAC GATGTGTACT GGGAGTGTGT GCTGCGACTC
    AACAAACAGC CAGATCTGGC ACTGCTGGCT TTCCTCGGGG TCCAACAGAA GTTCTGGCCT
    GTAACTGTTA CAATACATGG AGAAAAACAA CAGGTTCTCT CAAGCACTAA AGACGCCTGT
    TTCGCCTCAG CAGTTGAGAC TTTGCAGCAA ATAAGCACAA CGTTCACACC CTCAGACAAG
    CTTCAGGTGA TCCAGCTGAC CTTCGAGGAA ATAACCCAGG AAGTGCTCGC ACTCCTGAAA
    CAGGATTTCT TGTGGTCTAT GGATGACCTG TTTCCTGTCT TCCTCTTTGT TGTTCTGCGT
    GCACGGATAA GGAATCTGGG GTCAGAGGTT AGTCTCATTG AGGACCTAAT GGATCCATGT
    GTACAACATG GAGAGCATGG CATCATGTTC ACCACCCTCA AGGCGTGCTA CTATCAAATC
    CAGCATGAGA AGGTCACGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49117
    Clone ID Related Accession (Same CDS sequence) XM_009302175.3 , XM_009302175.2
    Accession Version XM_009302175.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5121bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product alsin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302175.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
    ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG
    CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC
    AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG
    GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC
    GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG
    TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG
    CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT
    TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA
    ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG
    GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG
    CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA
    GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG
    GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG
    AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC
    AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT
    CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT
    GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG
    CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG
    TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA
    ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC
    CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT
    GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC
    ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA
    GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG
    CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG ACAATGCAGA GTCTGAGGTG
    AAGGCGTCGT CATCCAACAC CACAACTGAA GTATCTCCTC GCCTCCTACG GAGAACAAGT
    CGCCCCCGTA TGCGGGCTGT CCCGCTCGCT TCAGGAGGTA TACCTGAGAC TGATGCCCAC
    CTGCCTTCCT TACAGACGGA AGTGTGGAGC TGGGGACAAG GCCAGGATGG ACAACTTGGA
    CATGGAGACC TCCTTCCCAG ACTGCAGCCT GATTGTGTAA AGAGTCTAAA TGGTAAGGAG
    GTGCTGAAAG TGGCTGCAGG GGCTCATCAC TCTCTCGCTC TGACAGCACA GTCACAGGTT
    TTCTCCTGGG GTTGTAACAC TTCTGGACAG CTGGGACACA TGGAATCGCC CACCACCTTC
    CCTCAATTGA CCAAGCTGTC AGATGGGATT CGTGTGTGGG ATATTGGGGC AGGTCTGCAG
    CACACTCTCT TATTGGCTGA CGGAGACTGT ATTCAGCCAA TCCTGTACTA CAGCGGACAG
    CAGGTGAAGG AAGTGCCGGA GCCAACAGAG GAAGAGGAGC AGACTGCAGA ATACACGCAA
    CAACCTGTGC TGCTGCCCTT CTGTATGAAC TTGGGCTATG TAAGCAGTGT GTATGCAGGT
    GGACGGACGT GTGCAGCGCT GACGGATTGT AATGTCATGG GTTTTATTGC TAGTCTGCAT
    GAGCTGGCGG CTGCAGAGAG AAAATATTAC TGCAAACTCA GCAATATCAA GAGTCTTCTG
    CTCCAGCCAT TGCTCAAGCT GGATTCTCTA AGCTCTGCCC TGGGTCAGTC ATCTGCAGGG
    CTGTTGCAGA ATCTGATTGG TCGAATGAGT CGACTGTCTC AGCTTACAGG TCAAAACTCG
    GCCTCTCTGA CCTCGTTCCT GCAGCGTTCA CGAGACATCC GAGGTCTTGT TATTCTTGAA
    GATGCACATC TGTTCCTGGA CACATATTCA GAGTACTCTT CTGCAGTTGG TGATCTTTTA
    GTCATGGGAG GATTTCAAGC CCTTGTCAAA CCTTGCCATG ATGTGTTTGG GAAAGGAGCA
    GAGGTTGTTC AGAGGTTGTC TGAATGTTCT GAAGAAGGTT TGACCTTGGC AGATGTTCTG
    GCGCATCTCT TTTACCTGCC CATAAAACAC CTGCATGAAT ATGGACGACT GCTGCTTAAA
    CTGGCTACCT GCTATGAGGT GAGTTCTGTG GACTATCAGA AGCTGCAGGA TGCCTGCTCA
    AAGTTCGAAT CCATGGCTCT TCATCTAAAG AGGAGGAGAA AGGAAGCTGA ATACACGCTC
    CACTTCTGGA AGAGTTTTCC TGGAAAGATG ACCGACTCCC TGCGTAAACC GTCTCGTCGG
    CTGATCTGTG AAAGCAGTAA TAAGGCTTTG ACTCTTCAAA ACGCAGGACG TTTTTCCGTC
    AACTGGTTCA TCCTCTTCAA CGACGCACTC GTTCATGCAC AGGGTATGGT CCCCTATAAG
    AGTCTTTTTT CCACCCATCA CATTTTTCCA TTGGCTACGC TGTGGGTGGA GCCAATTTCT
    GAGGAGAGCA CAGGCGTATA TGGCTTAAAA GTGACTTCGC CTGAGGAAAG CTTCGCTCTG
    CTGGCTTCAT CACCAGCTGA AAAAGCAAAG TGGCTTCGTT CTATAAACCA AGCGGTAGAG
    CAGGCGCTCA GTGGATTGGG GCATGATGGG ATACCTCCTT CTACAGGGAT GACGCAGAGA
    GCAGATCCAC CCATCTCCCG GACAGCATCC TACACCTTCT ACAAAGACAG TCGGCTAAAG
    GATGCCAAAT ATGATGGCCG ATGGGTCTCA GGGAAACCCC ATGGGCGGGG CGTGGTCAAA
    TGGCCAGATG GACGAATGTA CACGGGGACA TTCAAGAACG GTTTTGAAGA TGGTTTTGGA
    GATTACGTTG TGTCCAATAA AACCCTGAAC AGCTGTGATC ATTATCAAGG CCAGTGGAAA
    GATGGCAAGA TGCACGGTTT TGGAACATTC AGATATGCCT CTGGTGAAGT GTACGAAGGC
    TCATTTCAAG ACAACATGCG TCATGGTCAT GGGATGCTGC GTAGTGGGAA GCTGAATTCC
    ACCTCTCCCA GTGTCTTTAT CGGCCAGTGG CAGTACGACA AAAAGTCTGG CTATGGAGTT
    TTTGATGACA TCACACGAGG AGAGAAGTAT ATGGGCATGT GGCTGGATGA TCAACGGCAG
    GGGAACGGAG TTGTTGTTAC ACAATTTGGC CTATACTATG AAGGTGCATT CAGCAACAAC
    AAAATGATGG GTACTGGGGT GTTACTCTCT GAAGATGACA CTACATTTGA AGGAGAGTTT
    TTAGAAGACT GGACTCTTAA TGGAAAGGGT GTGTTGACCA TGCCGAATGG AGACTATATT
    GAAGGCTCTT TTTGTGGAGT TTGGGGAACC GGGCTAAAGA TGTCAGGTTC CTACTACAAA
    CCCAGCCTGT ACGACTCAGA CAAGGAGAAG GGTCATGCAC TTAAACTGGG CCGGTTAGCA
    GTTCACTCTG AAGAGAAGTG GAAGGCTGTG TTTTTGGAGT GCTGGAACAG GCTCGGTTGT
    GATTCCCCTG TACAGGGGCA AACCACGACA GCGTGGGACA ACATTGCCAT TGCACTCACT
    GCTAACCTGA GACAGCAGAG AGACAGTCCA GAGTTGCTGA GCCGCTCACA TCACAAAACA
    CTAGAGAGTC TTGAGTGCAT CCCTCAACAT GTGGGTCCTG TAACATTAGA GGTGTACCAC
    ACGATCCGTC GGTACCTGGT GAAGGCATGT GATACTCCTC TGCATCCTCT GGGTCGGCTG
    GTGGAGACGC TGGTTTCGGT ATACCGGATG ACGTATGTTG GTGTTGGAGC AAACCGCAGG
    TTATTACCGC AGGCCGTCAA TGAGATCACG TCCTATCTCA GCCGTATCTT CCAGCTCGTC
    AGATTTCTCT TTCCCGAACT GCCAGAAGAA GGAGGTTCAC TAGCCGACCC TCCAAAAGAG
    AAGGTCGGCA CAGATGCTGA TGGAAAGCTG CTGGAGTCTC CTAAACCTGG GTGTGTGGTC
    AGCAGCTCTG CTCTCCTCCT GCCGGTGCTG TTGCCACGTC TCTATCCTCC TCTCTTCACG
    CTCTACGCTC TAGAGAAAGA GAAAGAGGAC GATGTGTACT GGGAGTGTGT GCTGCGACTC
    AACAAACAGC CAGATCTGGC ACTGCTGGCT TTCCTCGGGG TCCAACAGAA GTTCTGGCCT
    GTAACTGTTA CAATACATGG AGAAAAACAA CAGGTTCTCT CAAGCACTAA AGACGCCTGT
    TTCGCCTCAG CAGTTGAGAC TTTGCAGCAA ATAAGCACAA CGTTCACACC CTCAGACAAG
    CTTCAGGTGA TCCAGCTGAC CTTCGAGGAA ATAACCCAGG AAGTGCTCGC ACTCCTGAAA
    CAGGATTTCT TGTGGTCTAT GGATGACCTG TTTCCTGTCT TCCTCTTTGT TGTTCTGCGT
    GCACGGATAA GGAATCTGGG GTCAGAGGTT AGTCTCATTG AGGACCTAAT GGATCCATGT
    GTACAACATG GAGAGCATGG CATCATGTTC ACCACCCTCA AGGCGTGCTA CTATCAAATC
    CAGCATGAGA AGGTCACGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009300450.1
    CDS250..5370
    Translation

    Target ORF information:

    RefSeq Version XM_009302175.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009302175.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
    ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG
    CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC
    AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG
    GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC
    GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG
    TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG
    CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT
    TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA
    ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG
    GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG
    CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA
    GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG
    GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG
    AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC
    AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT
    CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT
    GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG
    CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG
    TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA
    ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC
    CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT
    GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC
    ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA
    GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG
    CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG ACAATGCAGA GTCTGAGGTG
    AAGGCGTCGT CATCCAACAC CACAACTGAA GTATCTCCTC GCCTCCTACG GAGAACAAGT
    CGCCCCCGTA TGCGGGCTGT CCCGCTCGCT TCAGGAGGTA TACCTGAGAC TGATGCCCAC
    CTGCCTTCCT TACAGACGGA AGTGTGGAGC TGGGGACAAG GCCAGGATGG ACAACTTGGA
    CATGGAGACC TCCTTCCCAG ACTGCAGCCT GATTGTGTAA AGAGTCTAAA TGGTAAGGAG
    GTGCTGAAAG TGGCTGCAGG GGCTCATCAC TCTCTCGCTC TGACAGCACA GTCACAGGTT
    TTCTCCTGGG GTTGTAACAC TTCTGGACAG CTGGGACACA TGGAATCGCC CACCACCTTC
    CCTCAATTGA CCAAGCTGTC AGATGGGATT CGTGTGTGGG ATATTGGGGC AGGTCTGCAG
    CACACTCTCT TATTGGCTGA CGGAGACTGT ATTCAGCCAA TCCTGTACTA CAGCGGACAG
    CAGGTGAAGG AAGTGCCGGA GCCAACAGAG GAAGAGGAGC AGACTGCAGA ATACACGCAA
    CAACCTGTGC TGCTGCCCTT CTGTATGAAC TTGGGCTATG TAAGCAGTGT GTATGCAGGT
    GGACGGACGT GTGCAGCGCT GACGGATTGT AATGTCATGG GTTTTATTGC TAGTCTGCAT
    GAGCTGGCGG CTGCAGAGAG AAAATATTAC TGCAAACTCA GCAATATCAA GAGTCTTCTG
    CTCCAGCCAT TGCTCAAGCT GGATTCTCTA AGCTCTGCCC TGGGTCAGTC ATCTGCAGGG
    CTGTTGCAGA ATCTGATTGG TCGAATGAGT CGACTGTCTC AGCTTACAGG TCAAAACTCG
    GCCTCTCTGA CCTCGTTCCT GCAGCGTTCA CGAGACATCC GAGGTCTTGT TATTCTTGAA
    GATGCACATC TGTTCCTGGA CACATATTCA GAGTACTCTT CTGCAGTTGG TGATCTTTTA
    GTCATGGGAG GATTTCAAGC CCTTGTCAAA CCTTGCCATG ATGTGTTTGG GAAAGGAGCA
    GAGGTTGTTC AGAGGTTGTC TGAATGTTCT GAAGAAGGTT TGACCTTGGC AGATGTTCTG
    GCGCATCTCT TTTACCTGCC CATAAAACAC CTGCATGAAT ATGGACGACT GCTGCTTAAA
    CTGGCTACCT GCTATGAGGT GAGTTCTGTG GACTATCAGA AGCTGCAGGA TGCCTGCTCA
    AAGTTCGAAT CCATGGCTCT TCATCTAAAG AGGAGGAGAA AGGAAGCTGA ATACACGCTC
    CACTTCTGGA AGAGTTTTCC TGGAAAGATG ACCGACTCCC TGCGTAAACC GTCTCGTCGG
    CTGATCTGTG AAAGCAGTAA TAAGGCTTTG ACTCTTCAAA ACGCAGGACG TTTTTCCGTC
    AACTGGTTCA TCCTCTTCAA CGACGCACTC GTTCATGCAC AGGGTATGGT CCCCTATAAG
    AGTCTTTTTT CCACCCATCA CATTTTTCCA TTGGCTACGC TGTGGGTGGA GCCAATTTCT
    GAGGAGAGCA CAGGCGTATA TGGCTTAAAA GTGACTTCGC CTGAGGAAAG CTTCGCTCTG
    CTGGCTTCAT CACCAGCTGA AAAAGCAAAG TGGCTTCGTT CTATAAACCA AGCGGTAGAG
    CAGGCGCTCA GTGGATTGGG GCATGATGGG ATACCTCCTT CTACAGGGAT GACGCAGAGA
    GCAGATCCAC CCATCTCCCG GACAGCATCC TACACCTTCT ACAAAGACAG TCGGCTAAAG
    GATGCCAAAT ATGATGGCCG ATGGGTCTCA GGGAAACCCC ATGGGCGGGG CGTGGTCAAA
    TGGCCAGATG GACGAATGTA CACGGGGACA TTCAAGAACG GTTTTGAAGA TGGTTTTGGA
    GATTACGTTG TGTCCAATAA AACCCTGAAC AGCTGTGATC ATTATCAAGG CCAGTGGAAA
    GATGGCAAGA TGCACGGTTT TGGAACATTC AGATATGCCT CTGGTGAAGT GTACGAAGGC
    TCATTTCAAG ACAACATGCG TCATGGTCAT GGGATGCTGC GTAGTGGGAA GCTGAATTCC
    ACCTCTCCCA GTGTCTTTAT CGGCCAGTGG CAGTACGACA AAAAGTCTGG CTATGGAGTT
    TTTGATGACA TCACACGAGG AGAGAAGTAT ATGGGCATGT GGCTGGATGA TCAACGGCAG
    GGGAACGGAG TTGTTGTTAC ACAATTTGGC CTATACTATG AAGGTGCATT CAGCAACAAC
    AAAATGATGG GTACTGGGGT GTTACTCTCT GAAGATGACA CTACATTTGA AGGAGAGTTT
    TTAGAAGACT GGACTCTTAA TGGAAAGGGT GTGTTGACCA TGCCGAATGG AGACTATATT
    GAAGGCTCTT TTTGTGGAGT TTGGGGAACC GGGCTAAAGA TGTCAGGTTC CTACTACAAA
    CCCAGCCTGT ACGACTCAGA CAAGGAGAAG GGTCATGCAC TTAAACTGGG CCGGTTAGCA
    GTTCACTCTG AAGAGAAGTG GAAGGCTGTG TTTTTGGAGT GCTGGAACAG GCTCGGTTGT
    GATTCCCCTG TACAGGGGCA AACCACGACA GCGTGGGACA ACATTGCCAT TGCACTCACT
    GCTAACCTGA GACAGCAGAG AGACAGTCCA GAGTTGCTGA GCCGCTCACA TCACAAAACA
    CTAGAGAGTC TTGAGTGCAT CCCTCAACAT GTGGGTCCTG TAACATTAGA GGTGTACCAC
    ACGATCCGTC GGTACCTGGT GAAGGCATGT GATACTCCTC TGCATCCTCT GGGTCGGCTG
    GTGGAGACGC TGGTTTCGGT ATACCGGATG ACGTATGTTG GTGTTGGAGC AAACCGCAGG
    TTATTACCGC AGGCCGTCAA TGAGATCACG TCCTATCTCA GCCGTATCTT CCAGCTCGTC
    AGATTTCTCT TTCCCGAACT GCCAGAAGAA GGAGGTTCAC TAGCCGACCC TCCAAAAGAG
    AAGGTCGGCA CAGATGCTGA TGGAAAGCTG CTGGAGTCTC CTAAACCTGG GTGTGTGGTC
    AGCAGCTCTG CTCTCCTCCT GCCGGTGCTG TTGCCACGTC TCTATCCTCC TCTCTTCACG
    CTCTACGCTC TAGAGAAAGA GAAAGAGGAC GATGTGTACT GGGAGTGTGT GCTGCGACTC
    AACAAACAGC CAGATCTGGC ACTGCTGGCT TTCCTCGGGG TCCAACAGAA GTTCTGGCCT
    GTAACTGTTA CAATACATGG AGAAAAACAA CAGGTTCTCT CAAGCACTAA AGACGCCTGT
    TTCGCCTCAG CAGTTGAGAC TTTGCAGCAA ATAAGCACAA CGTTCACACC CTCAGACAAG
    CTTCAGGTGA TCCAGCTGAC CTTCGAGGAA ATAACCCAGG AAGTGCTCGC ACTCCTGAAA
    CAGGATTTCT TGTGGTCTAT GGATGACCTG TTTCCTGTCT TCCTCTTTGT TGTTCTGCGT
    GCACGGATAA GGAATCTGGG GTCAGAGGTT AGTCTCATTG AGGACCTAAT GGATCCATGT
    GTACAACATG GAGAGCATGG CATCATGTTC ACCACCCTCA AGGCGTGCTA CTATCAAATC
    CAGCATGAGA AGGTCACGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49118
    Clone ID Related Accession (Same CDS sequence) XM_009302176.2 , XM_009302176.3
    Accession Version XM_009302176.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product alsin isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302176.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
    ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG
    CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC
    AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG
    GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC
    GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG
    TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG
    CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT
    TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA
    ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG
    GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG
    CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA
    GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG
    GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG
    AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC
    AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT
    CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT
    GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG
    CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG
    TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA
    ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC
    CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT
    GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC
    ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA
    GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG
    CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG GTACACACGC TCTCCTTCTC
    TTACACCCAG TGTCCCGTTT CCTATCTCCT CGCCTCCTAC GGAGAACAAG TCGCCCCCGT
    ATGCGGGCTG TCCCGCTCGC TTCAGGAGGT ATACCTGAGA CTGATGCCCA CCTGCCTTCC
    TTACAGACGG AAGTGTGGAG CTGGGGACAA GGCCAGGATG GACAACTTGG ACATGGAGAC
    CTCCTTCCCA GACTGCAGCC TGATTGTGTA AAGAGTCTAA ATGGTAAGGA GGTGCTGAAA
    GTGGCTGCAG GGGCTCATCA CTCTCTCGCT CTGACAGCAC AGTCACAGGT TTTCTCCTGG
    GGTTGTAACA CTTCTGGACA GCTGGGACAC ATGGAATCGC CCACCACCTT CCCTCAATTG
    ACCAAGCTGT CAGATGGGAT TCGTGTGTGG GATATTGGGG CAGGTCTGCA GCACACTCTC
    TTATTGGCTG ACGGAGACTG TATTCAGCCA ATCCTGTACT ACAGCGGACA GCAGGTGAAG
    GAAGTGCCGG AGCCAACAGA GGAAGAGGAG CAGACTGCAG AATACACGCA ACAACCTGTG
    CTGCTGCCCT TCTGTATGAA CTTGGGCTAT GTAAGCAGTG TGTATGCAGG TGGACGGACG
    TGTGCAGCGC TGACGGATTG TAATGTCATG GGTTTTATTG CTAGTCTGCA TGAGCTGGCG
    GCTGCAGAGA GAAAATATTA CTGCAAACTC AGCAATATCA AGAGTCTTCT GCTCCAGCCA
    TTGCTCAAGC TGGATTCTCT AAGCTCTGCC CTGGGTCAGT CATCTGCAGG GCTGTTGCAG
    AATCTGATTG GTCGAATGAG TCGACTGTCT CAGCTTACAG GTCAAAACTC GGCCTCTCTG
    ACCTCGTTCC TGCAGCGTTC ACGAGACATC CGAGGTCTTG TTATTCTTGA AGATGCACAT
    CTGTTCCTGG ACACATATTC AGAGTACTCT TCTGCAGTTG GTGATCTTTT AGTCATGGGA
    GGATTTCAAG CCCTTGTCAA ACCTTGCCAT GATGTGTTTG GGAAAGGAGC AGAGGTTGTT
    CAGAGGTTGT CTGAATGTTC TGAAGAAGGT TTGACCTTGG CAGATGTTCT GGCGCATCTC
    TTTTACCTGC CCATAAAACA CCTGCATGAA TATGGACGAC TGCTGCTTAA ACTGGCTACC
    TGCTATGAGG TGAGTTCTGT GGACTATCAG AAGCTGCAGG ATGCCTGCTC AAAGTTCGAA
    TCCATGGCTC TTCATCTAAA GAGGAGGAGA AAGGAAGCTG AATACACGCT CCACTTCTGG
    AAGAGTTTTC CTGGAAAGAT GACCGACTCC CTGCGTAAAC CGTCTCGTCG GCTGATCTGT
    GAAAGCAGTA ATAAGGCTTT GACTCTTCAA AACGCAGGAC GTTTTTCCGT CAACTGGTTC
    ATCCTCTTCA ACGACGCACT CGTTCATGCA CAGGGTATGG TCCCCTATAA GAGTCTTTTT
    TCCACCCATC ACATTTTTCC ATTGGCTACG CTGTGGGTGG AGCCAATTTC TGAGGAGAGC
    ACAGGCGTAT ATGGCTTAAA AGTGACTTCG CCTGAGGAAA GCTTCGCTCT GCTGGCTTCA
    TCACCAGCTG AAAAAGCAAA GTGGCTTCGT TCTATAAACC AAGCGGTAGA GCAGGCGCTC
    AGTGGATTGG GGCATGATGG GATACCTCCT TCTACAGGGA TGACGCAGAG AGCAGATCCA
    CCCATCTCCC GGACAGCATC CTACACCTTC TACAAAGACA GTCGGCTAAA GGATGCCAAA
    TATGATGGCC GATGGGTCTC AGGGAAACCC CATGGGCGGG GCGTGGTCAA ATGGCCAGAT
    GGACGAATGT ACACGGGGAC ATTCAAGAAC GGTTTTGAAG ATGGTTTTGG AGATTACGTT
    GTGTCCAATA AAACCCTGAA CAGCTGTGAT CATTATCAAG GCCAGTGGAA AGATGGCAAG
    ATGCACGGTT TTGGAACATT CAGATATGCC TCTGGTGAAG TGTACGAAGG CTCATTTCAA
    GACAACATGC GTCATGGTCA TGGGATGCTG CGTAGTGGGA AGCTGAATTC CACCTCTCCC
    AGTGTCTTTA TCGGCCAGTG GCAGTACGAC AAAAAGTCTG GCTATGGAGT TTTTGATGAC
    ATCACACGAG GAGAGAAGTA TATGGGCATG TGGCTGGATG ATCAACGGCA GGGGAACGGA
    GTTGTTGTTA CACAATTTGG CCTATACTAT GAAGGTGCAT TCAGCAACAA CAAAATGATG
    GGTACTGGGG TGTTACTCTC TGAAGATGAC ACTACATTTG AAGGAGAGTT TTTAGAAGAC
    TGGACTCTTA ATGGAAAGGG TGTGTTGACC ATGCCGAATG GAGACTATAT TGAAGGCTCT
    TTTTGTGGAG TTTGGGGAAC CGGGCTAAAG ATGTCAGGTT CCTACTACAA ACCCAGCCTG
    TACGACTCAG ACAAGGAGAA GGGTCATGCA CTTAAACTGG GCCGGTTAGC AGTTCACTCT
    GAAGAGAAGT GGAAGGCTGT GTTTTTGGAG TGCTGGAACA GGCTCGGTTG TGATTCCCCT
    GTACAGGGGC AAACCACGAC AGCGTGGGAC AACATTGCCA TTGCACTCAC TGCTAACCTG
    AGACAGCAGA GAGACAGTCC AGAGTTGCTG AGCCGCTCAC ATCACAAAAC ACTAGAGAGT
    CTTGAGTGCA TCCCTCAACA TGTGGGTCCT GTAACATTAG AGGTGTACCA CACGATCCGT
    CGGTACCTGG TGAAGGCATG TGATACTCCT CTGCATCCTC TGGGTCGGCT GGTGGAGACG
    CTGGTTTCGG TATACCGGAT GACGTATGTT GGTGTTGGAG CAAACCGCAG GTTATTACCG
    CAGGCCGTCA ATGAGATCAC GTCCTATCTC AGCCGTATCT TCCAGCTCGT CAGATTTCTC
    TTTCCCGAAC TGCCAGAAGA AGGAGGTTCA CTAGCCGACC CTCCAAAAGA GAAGGTCGGC
    ACAGATGCTG ATGGAAAGCT GCTGGAGTCT CCTAAACCTG GGTGTGTGGT CAGCAGCTCT
    GCTCTCCTCC TGCCGGTGCT GTTGCCACGT CTCTATCCTC CTCTCTTCAC GCTCTACGCT
    CTAGAGAAAG AGAAAGAGGA CGATGTGTAC TGGGAGTGTG TGCTGCGACT CAACAAACAG
    CCAGATCTGG CACTGCTGGC TTTCCTCGGG GTCCAACAGA AGTTCTGGCC TGTAACTGTT
    ACAATACATG GAGAAAAACA ACAGGTTCTC TCAAGCACTA AAGACGCCTG TTTCGCCTCA
    GCAGTTGAGA CTTTGCAGCA AATAAGCACA ACGTTCACAC CCTCAGACAA GCTTCAGGTG
    ATCCAGCTGA CCTTCGAGGA AATAACCCAG GAAGTGCTCG CACTCCTGAA ACAGGATTTC
    TTGTGGTCTA TGGATGACCT GTTTCCTGTC TTCCTCTTTG TTGTTCTGCG TGCACGGATA
    AGGAATCTGG GGTCAGAGGT TAGTCTCATT GAGGACCTAA TGGATCCATG TGTACAACAT
    GGAGAGCATG GCATCATGTT CACCACCCTC AAGGCGTGCT ACTATCAAAT CCAGCATGAG
    AAGGTCACGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009300451.1
    CDS250..5361
    Translation

    Target ORF information:

    RefSeq Version XM_009302176.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009302176.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
    ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG
    CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC
    AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG
    GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC
    GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG
    TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG
    CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT
    TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA
    ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG
    GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG
    CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA
    GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG
    GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG
    AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC
    AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT
    CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT
    GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG
    CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG
    TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA
    ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC
    CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT
    GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC
    ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA
    GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG
    CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG GTACACACGC TCTCCTTCTC
    TTACACCCAG TGTCCCGTTT CCTATCTCCT CGCCTCCTAC GGAGAACAAG TCGCCCCCGT
    ATGCGGGCTG TCCCGCTCGC TTCAGGAGGT ATACCTGAGA CTGATGCCCA CCTGCCTTCC
    TTACAGACGG AAGTGTGGAG CTGGGGACAA GGCCAGGATG GACAACTTGG ACATGGAGAC
    CTCCTTCCCA GACTGCAGCC TGATTGTGTA AAGAGTCTAA ATGGTAAGGA GGTGCTGAAA
    GTGGCTGCAG GGGCTCATCA CTCTCTCGCT CTGACAGCAC AGTCACAGGT TTTCTCCTGG
    GGTTGTAACA CTTCTGGACA GCTGGGACAC ATGGAATCGC CCACCACCTT CCCTCAATTG
    ACCAAGCTGT CAGATGGGAT TCGTGTGTGG GATATTGGGG CAGGTCTGCA GCACACTCTC
    TTATTGGCTG ACGGAGACTG TATTCAGCCA ATCCTGTACT ACAGCGGACA GCAGGTGAAG
    GAAGTGCCGG AGCCAACAGA GGAAGAGGAG CAGACTGCAG AATACACGCA ACAACCTGTG
    CTGCTGCCCT TCTGTATGAA CTTGGGCTAT GTAAGCAGTG TGTATGCAGG TGGACGGACG
    TGTGCAGCGC TGACGGATTG TAATGTCATG GGTTTTATTG CTAGTCTGCA TGAGCTGGCG
    GCTGCAGAGA GAAAATATTA CTGCAAACTC AGCAATATCA AGAGTCTTCT GCTCCAGCCA
    TTGCTCAAGC TGGATTCTCT AAGCTCTGCC CTGGGTCAGT CATCTGCAGG GCTGTTGCAG
    AATCTGATTG GTCGAATGAG TCGACTGTCT CAGCTTACAG GTCAAAACTC GGCCTCTCTG
    ACCTCGTTCC TGCAGCGTTC ACGAGACATC CGAGGTCTTG TTATTCTTGA AGATGCACAT
    CTGTTCCTGG ACACATATTC AGAGTACTCT TCTGCAGTTG GTGATCTTTT AGTCATGGGA
    GGATTTCAAG CCCTTGTCAA ACCTTGCCAT GATGTGTTTG GGAAAGGAGC AGAGGTTGTT
    CAGAGGTTGT CTGAATGTTC TGAAGAAGGT TTGACCTTGG CAGATGTTCT GGCGCATCTC
    TTTTACCTGC CCATAAAACA CCTGCATGAA TATGGACGAC TGCTGCTTAA ACTGGCTACC
    TGCTATGAGG TGAGTTCTGT GGACTATCAG AAGCTGCAGG ATGCCTGCTC AAAGTTCGAA
    TCCATGGCTC TTCATCTAAA GAGGAGGAGA AAGGAAGCTG AATACACGCT CCACTTCTGG
    AAGAGTTTTC CTGGAAAGAT GACCGACTCC CTGCGTAAAC CGTCTCGTCG GCTGATCTGT
    GAAAGCAGTA ATAAGGCTTT GACTCTTCAA AACGCAGGAC GTTTTTCCGT CAACTGGTTC
    ATCCTCTTCA ACGACGCACT CGTTCATGCA CAGGGTATGG TCCCCTATAA GAGTCTTTTT
    TCCACCCATC ACATTTTTCC ATTGGCTACG CTGTGGGTGG AGCCAATTTC TGAGGAGAGC
    ACAGGCGTAT ATGGCTTAAA AGTGACTTCG CCTGAGGAAA GCTTCGCTCT GCTGGCTTCA
    TCACCAGCTG AAAAAGCAAA GTGGCTTCGT TCTATAAACC AAGCGGTAGA GCAGGCGCTC
    AGTGGATTGG GGCATGATGG GATACCTCCT TCTACAGGGA TGACGCAGAG AGCAGATCCA
    CCCATCTCCC GGACAGCATC CTACACCTTC TACAAAGACA GTCGGCTAAA GGATGCCAAA
    TATGATGGCC GATGGGTCTC AGGGAAACCC CATGGGCGGG GCGTGGTCAA ATGGCCAGAT
    GGACGAATGT ACACGGGGAC ATTCAAGAAC GGTTTTGAAG ATGGTTTTGG AGATTACGTT
    GTGTCCAATA AAACCCTGAA CAGCTGTGAT CATTATCAAG GCCAGTGGAA AGATGGCAAG
    ATGCACGGTT TTGGAACATT CAGATATGCC TCTGGTGAAG TGTACGAAGG CTCATTTCAA
    GACAACATGC GTCATGGTCA TGGGATGCTG CGTAGTGGGA AGCTGAATTC CACCTCTCCC
    AGTGTCTTTA TCGGCCAGTG GCAGTACGAC AAAAAGTCTG GCTATGGAGT TTTTGATGAC
    ATCACACGAG GAGAGAAGTA TATGGGCATG TGGCTGGATG ATCAACGGCA GGGGAACGGA
    GTTGTTGTTA CACAATTTGG CCTATACTAT GAAGGTGCAT TCAGCAACAA CAAAATGATG
    GGTACTGGGG TGTTACTCTC TGAAGATGAC ACTACATTTG AAGGAGAGTT TTTAGAAGAC
    TGGACTCTTA ATGGAAAGGG TGTGTTGACC ATGCCGAATG GAGACTATAT TGAAGGCTCT
    TTTTGTGGAG TTTGGGGAAC CGGGCTAAAG ATGTCAGGTT CCTACTACAA ACCCAGCCTG
    TACGACTCAG ACAAGGAGAA GGGTCATGCA CTTAAACTGG GCCGGTTAGC AGTTCACTCT
    GAAGAGAAGT GGAAGGCTGT GTTTTTGGAG TGCTGGAACA GGCTCGGTTG TGATTCCCCT
    GTACAGGGGC AAACCACGAC AGCGTGGGAC AACATTGCCA TTGCACTCAC TGCTAACCTG
    AGACAGCAGA GAGACAGTCC AGAGTTGCTG AGCCGCTCAC ATCACAAAAC ACTAGAGAGT
    CTTGAGTGCA TCCCTCAACA TGTGGGTCCT GTAACATTAG AGGTGTACCA CACGATCCGT
    CGGTACCTGG TGAAGGCATG TGATACTCCT CTGCATCCTC TGGGTCGGCT GGTGGAGACG
    CTGGTTTCGG TATACCGGAT GACGTATGTT GGTGTTGGAG CAAACCGCAG GTTATTACCG
    CAGGCCGTCA ATGAGATCAC GTCCTATCTC AGCCGTATCT TCCAGCTCGT CAGATTTCTC
    TTTCCCGAAC TGCCAGAAGA AGGAGGTTCA CTAGCCGACC CTCCAAAAGA GAAGGTCGGC
    ACAGATGCTG ATGGAAAGCT GCTGGAGTCT CCTAAACCTG GGTGTGTGGT CAGCAGCTCT
    GCTCTCCTCC TGCCGGTGCT GTTGCCACGT CTCTATCCTC CTCTCTTCAC GCTCTACGCT
    CTAGAGAAAG AGAAAGAGGA CGATGTGTAC TGGGAGTGTG TGCTGCGACT CAACAAACAG
    CCAGATCTGG CACTGCTGGC TTTCCTCGGG GTCCAACAGA AGTTCTGGCC TGTAACTGTT
    ACAATACATG GAGAAAAACA ACAGGTTCTC TCAAGCACTA AAGACGCCTG TTTCGCCTCA
    GCAGTTGAGA CTTTGCAGCA AATAAGCACA ACGTTCACAC CCTCAGACAA GCTTCAGGTG
    ATCCAGCTGA CCTTCGAGGA AATAACCCAG GAAGTGCTCG CACTCCTGAA ACAGGATTTC
    TTGTGGTCTA TGGATGACCT GTTTCCTGTC TTCCTCTTTG TTGTTCTGCG TGCACGGATA
    AGGAATCTGG GGTCAGAGGT TAGTCTCATT GAGGACCTAA TGGATCCATG TGTACAACAT
    GGAGAGCATG GCATCATGTT CACCACCCTC AAGGCGTGCT ACTATCAAAT CCAGCATGAG
    AAGGTCACGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49118
    Clone ID Related Accession (Same CDS sequence) XM_009302176.2 , XM_009302176.3
    Accession Version XM_009302176.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product alsin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009302176.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
    ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG
    CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC
    AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG
    GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC
    GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG
    TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG
    CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT
    TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA
    ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG
    GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG
    CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA
    GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG
    GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG
    AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC
    AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT
    CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT
    GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG
    CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG
    TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA
    ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC
    CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT
    GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC
    ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA
    GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG
    CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG GTACACACGC TCTCCTTCTC
    TTACACCCAG TGTCCCGTTT CCTATCTCCT CGCCTCCTAC GGAGAACAAG TCGCCCCCGT
    ATGCGGGCTG TCCCGCTCGC TTCAGGAGGT ATACCTGAGA CTGATGCCCA CCTGCCTTCC
    TTACAGACGG AAGTGTGGAG CTGGGGACAA GGCCAGGATG GACAACTTGG ACATGGAGAC
    CTCCTTCCCA GACTGCAGCC TGATTGTGTA AAGAGTCTAA ATGGTAAGGA GGTGCTGAAA
    GTGGCTGCAG GGGCTCATCA CTCTCTCGCT CTGACAGCAC AGTCACAGGT TTTCTCCTGG
    GGTTGTAACA CTTCTGGACA GCTGGGACAC ATGGAATCGC CCACCACCTT CCCTCAATTG
    ACCAAGCTGT CAGATGGGAT TCGTGTGTGG GATATTGGGG CAGGTCTGCA GCACACTCTC
    TTATTGGCTG ACGGAGACTG TATTCAGCCA ATCCTGTACT ACAGCGGACA GCAGGTGAAG
    GAAGTGCCGG AGCCAACAGA GGAAGAGGAG CAGACTGCAG AATACACGCA ACAACCTGTG
    CTGCTGCCCT TCTGTATGAA CTTGGGCTAT GTAAGCAGTG TGTATGCAGG TGGACGGACG
    TGTGCAGCGC TGACGGATTG TAATGTCATG GGTTTTATTG CTAGTCTGCA TGAGCTGGCG
    GCTGCAGAGA GAAAATATTA CTGCAAACTC AGCAATATCA AGAGTCTTCT GCTCCAGCCA
    TTGCTCAAGC TGGATTCTCT AAGCTCTGCC CTGGGTCAGT CATCTGCAGG GCTGTTGCAG
    AATCTGATTG GTCGAATGAG TCGACTGTCT CAGCTTACAG GTCAAAACTC GGCCTCTCTG
    ACCTCGTTCC TGCAGCGTTC ACGAGACATC CGAGGTCTTG TTATTCTTGA AGATGCACAT
    CTGTTCCTGG ACACATATTC AGAGTACTCT TCTGCAGTTG GTGATCTTTT AGTCATGGGA
    GGATTTCAAG CCCTTGTCAA ACCTTGCCAT GATGTGTTTG GGAAAGGAGC AGAGGTTGTT
    CAGAGGTTGT CTGAATGTTC TGAAGAAGGT TTGACCTTGG CAGATGTTCT GGCGCATCTC
    TTTTACCTGC CCATAAAACA CCTGCATGAA TATGGACGAC TGCTGCTTAA ACTGGCTACC
    TGCTATGAGG TGAGTTCTGT GGACTATCAG AAGCTGCAGG ATGCCTGCTC AAAGTTCGAA
    TCCATGGCTC TTCATCTAAA GAGGAGGAGA AAGGAAGCTG AATACACGCT CCACTTCTGG
    AAGAGTTTTC CTGGAAAGAT GACCGACTCC CTGCGTAAAC CGTCTCGTCG GCTGATCTGT
    GAAAGCAGTA ATAAGGCTTT GACTCTTCAA AACGCAGGAC GTTTTTCCGT CAACTGGTTC
    ATCCTCTTCA ACGACGCACT CGTTCATGCA CAGGGTATGG TCCCCTATAA GAGTCTTTTT
    TCCACCCATC ACATTTTTCC ATTGGCTACG CTGTGGGTGG AGCCAATTTC TGAGGAGAGC
    ACAGGCGTAT ATGGCTTAAA AGTGACTTCG CCTGAGGAAA GCTTCGCTCT GCTGGCTTCA
    TCACCAGCTG AAAAAGCAAA GTGGCTTCGT TCTATAAACC AAGCGGTAGA GCAGGCGCTC
    AGTGGATTGG GGCATGATGG GATACCTCCT TCTACAGGGA TGACGCAGAG AGCAGATCCA
    CCCATCTCCC GGACAGCATC CTACACCTTC TACAAAGACA GTCGGCTAAA GGATGCCAAA
    TATGATGGCC GATGGGTCTC AGGGAAACCC CATGGGCGGG GCGTGGTCAA ATGGCCAGAT
    GGACGAATGT ACACGGGGAC ATTCAAGAAC GGTTTTGAAG ATGGTTTTGG AGATTACGTT
    GTGTCCAATA AAACCCTGAA CAGCTGTGAT CATTATCAAG GCCAGTGGAA AGATGGCAAG
    ATGCACGGTT TTGGAACATT CAGATATGCC TCTGGTGAAG TGTACGAAGG CTCATTTCAA
    GACAACATGC GTCATGGTCA TGGGATGCTG CGTAGTGGGA AGCTGAATTC CACCTCTCCC
    AGTGTCTTTA TCGGCCAGTG GCAGTACGAC AAAAAGTCTG GCTATGGAGT TTTTGATGAC
    ATCACACGAG GAGAGAAGTA TATGGGCATG TGGCTGGATG ATCAACGGCA GGGGAACGGA
    GTTGTTGTTA CACAATTTGG CCTATACTAT GAAGGTGCAT TCAGCAACAA CAAAATGATG
    GGTACTGGGG TGTTACTCTC TGAAGATGAC ACTACATTTG AAGGAGAGTT TTTAGAAGAC
    TGGACTCTTA ATGGAAAGGG TGTGTTGACC ATGCCGAATG GAGACTATAT TGAAGGCTCT
    TTTTGTGGAG TTTGGGGAAC CGGGCTAAAG ATGTCAGGTT CCTACTACAA ACCCAGCCTG
    TACGACTCAG ACAAGGAGAA GGGTCATGCA CTTAAACTGG GCCGGTTAGC AGTTCACTCT
    GAAGAGAAGT GGAAGGCTGT GTTTTTGGAG TGCTGGAACA GGCTCGGTTG TGATTCCCCT
    GTACAGGGGC AAACCACGAC AGCGTGGGAC AACATTGCCA TTGCACTCAC TGCTAACCTG
    AGACAGCAGA GAGACAGTCC AGAGTTGCTG AGCCGCTCAC ATCACAAAAC ACTAGAGAGT
    CTTGAGTGCA TCCCTCAACA TGTGGGTCCT GTAACATTAG AGGTGTACCA CACGATCCGT
    CGGTACCTGG TGAAGGCATG TGATACTCCT CTGCATCCTC TGGGTCGGCT GGTGGAGACG
    CTGGTTTCGG TATACCGGAT GACGTATGTT GGTGTTGGAG CAAACCGCAG GTTATTACCG
    CAGGCCGTCA ATGAGATCAC GTCCTATCTC AGCCGTATCT TCCAGCTCGT CAGATTTCTC
    TTTCCCGAAC TGCCAGAAGA AGGAGGTTCA CTAGCCGACC CTCCAAAAGA GAAGGTCGGC
    ACAGATGCTG ATGGAAAGCT GCTGGAGTCT CCTAAACCTG GGTGTGTGGT CAGCAGCTCT
    GCTCTCCTCC TGCCGGTGCT GTTGCCACGT CTCTATCCTC CTCTCTTCAC GCTCTACGCT
    CTAGAGAAAG AGAAAGAGGA CGATGTGTAC TGGGAGTGTG TGCTGCGACT CAACAAACAG
    CCAGATCTGG CACTGCTGGC TTTCCTCGGG GTCCAACAGA AGTTCTGGCC TGTAACTGTT
    ACAATACATG GAGAAAAACA ACAGGTTCTC TCAAGCACTA AAGACGCCTG TTTCGCCTCA
    GCAGTTGAGA CTTTGCAGCA AATAAGCACA ACGTTCACAC CCTCAGACAA GCTTCAGGTG
    ATCCAGCTGA CCTTCGAGGA AATAACCCAG GAAGTGCTCG CACTCCTGAA ACAGGATTTC
    TTGTGGTCTA TGGATGACCT GTTTCCTGTC TTCCTCTTTG TTGTTCTGCG TGCACGGATA
    AGGAATCTGG GGTCAGAGGT TAGTCTCATT GAGGACCTAA TGGATCCATG TGTACAACAT
    GGAGAGCATG GCATCATGTT CACCACCCTC AAGGCGTGCT ACTATCAAAT CCAGCATGAG
    AAGGTCACGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009300451.1
    CDS270..5381
    Translation

    Target ORF information:

    RefSeq Version XM_009302176.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009302176.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
    ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG
    CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC
    AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG
    GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC
    GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG
    TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG
    CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT
    TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA
    ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG
    GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG
    CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA
    GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG
    GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG
    AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC
    AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT
    CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT
    GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG
    CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG
    TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA
    ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC
    CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT
    GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC
    ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA
    GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG
    CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG GTACACACGC TCTCCTTCTC
    TTACACCCAG TGTCCCGTTT CCTATCTCCT CGCCTCCTAC GGAGAACAAG TCGCCCCCGT
    ATGCGGGCTG TCCCGCTCGC TTCAGGAGGT ATACCTGAGA CTGATGCCCA CCTGCCTTCC
    TTACAGACGG AAGTGTGGAG CTGGGGACAA GGCCAGGATG GACAACTTGG ACATGGAGAC
    CTCCTTCCCA GACTGCAGCC TGATTGTGTA AAGAGTCTAA ATGGTAAGGA GGTGCTGAAA
    GTGGCTGCAG GGGCTCATCA CTCTCTCGCT CTGACAGCAC AGTCACAGGT TTTCTCCTGG
    GGTTGTAACA CTTCTGGACA GCTGGGACAC ATGGAATCGC CCACCACCTT CCCTCAATTG
    ACCAAGCTGT CAGATGGGAT TCGTGTGTGG GATATTGGGG CAGGTCTGCA GCACACTCTC
    TTATTGGCTG ACGGAGACTG TATTCAGCCA ATCCTGTACT ACAGCGGACA GCAGGTGAAG
    GAAGTGCCGG AGCCAACAGA GGAAGAGGAG CAGACTGCAG AATACACGCA ACAACCTGTG
    CTGCTGCCCT TCTGTATGAA CTTGGGCTAT GTAAGCAGTG TGTATGCAGG TGGACGGACG
    TGTGCAGCGC TGACGGATTG TAATGTCATG GGTTTTATTG CTAGTCTGCA TGAGCTGGCG
    GCTGCAGAGA GAAAATATTA CTGCAAACTC AGCAATATCA AGAGTCTTCT GCTCCAGCCA
    TTGCTCAAGC TGGATTCTCT AAGCTCTGCC CTGGGTCAGT CATCTGCAGG GCTGTTGCAG
    AATCTGATTG GTCGAATGAG TCGACTGTCT CAGCTTACAG GTCAAAACTC GGCCTCTCTG
    ACCTCGTTCC TGCAGCGTTC ACGAGACATC CGAGGTCTTG TTATTCTTGA AGATGCACAT
    CTGTTCCTGG ACACATATTC AGAGTACTCT TCTGCAGTTG GTGATCTTTT AGTCATGGGA
    GGATTTCAAG CCCTTGTCAA ACCTTGCCAT GATGTGTTTG GGAAAGGAGC AGAGGTTGTT
    CAGAGGTTGT CTGAATGTTC TGAAGAAGGT TTGACCTTGG CAGATGTTCT GGCGCATCTC
    TTTTACCTGC CCATAAAACA CCTGCATGAA TATGGACGAC TGCTGCTTAA ACTGGCTACC
    TGCTATGAGG TGAGTTCTGT GGACTATCAG AAGCTGCAGG ATGCCTGCTC AAAGTTCGAA
    TCCATGGCTC TTCATCTAAA GAGGAGGAGA AAGGAAGCTG AATACACGCT CCACTTCTGG
    AAGAGTTTTC CTGGAAAGAT GACCGACTCC CTGCGTAAAC CGTCTCGTCG GCTGATCTGT
    GAAAGCAGTA ATAAGGCTTT GACTCTTCAA AACGCAGGAC GTTTTTCCGT CAACTGGTTC
    ATCCTCTTCA ACGACGCACT CGTTCATGCA CAGGGTATGG TCCCCTATAA GAGTCTTTTT
    TCCACCCATC ACATTTTTCC ATTGGCTACG CTGTGGGTGG AGCCAATTTC TGAGGAGAGC
    ACAGGCGTAT ATGGCTTAAA AGTGACTTCG CCTGAGGAAA GCTTCGCTCT GCTGGCTTCA
    TCACCAGCTG AAAAAGCAAA GTGGCTTCGT TCTATAAACC AAGCGGTAGA GCAGGCGCTC
    AGTGGATTGG GGCATGATGG GATACCTCCT TCTACAGGGA TGACGCAGAG AGCAGATCCA
    CCCATCTCCC GGACAGCATC CTACACCTTC TACAAAGACA GTCGGCTAAA GGATGCCAAA
    TATGATGGCC GATGGGTCTC AGGGAAACCC CATGGGCGGG GCGTGGTCAA ATGGCCAGAT
    GGACGAATGT ACACGGGGAC ATTCAAGAAC GGTTTTGAAG ATGGTTTTGG AGATTACGTT
    GTGTCCAATA AAACCCTGAA CAGCTGTGAT CATTATCAAG GCCAGTGGAA AGATGGCAAG
    ATGCACGGTT TTGGAACATT CAGATATGCC TCTGGTGAAG TGTACGAAGG CTCATTTCAA
    GACAACATGC GTCATGGTCA TGGGATGCTG CGTAGTGGGA AGCTGAATTC CACCTCTCCC
    AGTGTCTTTA TCGGCCAGTG GCAGTACGAC AAAAAGTCTG GCTATGGAGT TTTTGATGAC
    ATCACACGAG GAGAGAAGTA TATGGGCATG TGGCTGGATG ATCAACGGCA GGGGAACGGA
    GTTGTTGTTA CACAATTTGG CCTATACTAT GAAGGTGCAT TCAGCAACAA CAAAATGATG
    GGTACTGGGG TGTTACTCTC TGAAGATGAC ACTACATTTG AAGGAGAGTT TTTAGAAGAC
    TGGACTCTTA ATGGAAAGGG TGTGTTGACC ATGCCGAATG GAGACTATAT TGAAGGCTCT
    TTTTGTGGAG TTTGGGGAAC CGGGCTAAAG ATGTCAGGTT CCTACTACAA ACCCAGCCTG
    TACGACTCAG ACAAGGAGAA GGGTCATGCA CTTAAACTGG GCCGGTTAGC AGTTCACTCT
    GAAGAGAAGT GGAAGGCTGT GTTTTTGGAG TGCTGGAACA GGCTCGGTTG TGATTCCCCT
    GTACAGGGGC AAACCACGAC AGCGTGGGAC AACATTGCCA TTGCACTCAC TGCTAACCTG
    AGACAGCAGA GAGACAGTCC AGAGTTGCTG AGCCGCTCAC ATCACAAAAC ACTAGAGAGT
    CTTGAGTGCA TCCCTCAACA TGTGGGTCCT GTAACATTAG AGGTGTACCA CACGATCCGT
    CGGTACCTGG TGAAGGCATG TGATACTCCT CTGCATCCTC TGGGTCGGCT GGTGGAGACG
    CTGGTTTCGG TATACCGGAT GACGTATGTT GGTGTTGGAG CAAACCGCAG GTTATTACCG
    CAGGCCGTCA ATGAGATCAC GTCCTATCTC AGCCGTATCT TCCAGCTCGT CAGATTTCTC
    TTTCCCGAAC TGCCAGAAGA AGGAGGTTCA CTAGCCGACC CTCCAAAAGA GAAGGTCGGC
    ACAGATGCTG ATGGAAAGCT GCTGGAGTCT CCTAAACCTG GGTGTGTGGT CAGCAGCTCT
    GCTCTCCTCC TGCCGGTGCT GTTGCCACGT CTCTATCCTC CTCTCTTCAC GCTCTACGCT
    CTAGAGAAAG AGAAAGAGGA CGATGTGTAC TGGGAGTGTG TGCTGCGACT CAACAAACAG
    CCAGATCTGG CACTGCTGGC TTTCCTCGGG GTCCAACAGA AGTTCTGGCC TGTAACTGTT
    ACAATACATG GAGAAAAACA ACAGGTTCTC TCAAGCACTA AAGACGCCTG TTTCGCCTCA
    GCAGTTGAGA CTTTGCAGCA AATAAGCACA ACGTTCACAC CCTCAGACAA GCTTCAGGTG
    ATCCAGCTGA CCTTCGAGGA AATAACCCAG GAAGTGCTCG CACTCCTGAA ACAGGATTTC
    TTGTGGTCTA TGGATGACCT GTTTCCTGTC TTCCTCTTTG TTGTTCTGCG TGCACGGATA
    AGGAATCTGG GGTCAGAGGT TAGTCTCATT GAGGACCTAA TGGATCCATG TGTACAACAT
    GGAGAGCATG GCATCATGTT CACCACCCTC AAGGCGTGCT ACTATCAAAT CCAGCATGAG
    AAGGTCACGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49119
    Clone ID Related Accession (Same CDS sequence) XM_009302177.2 , XM_009302177.3
    Accession Version XM_009302177.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product alsin isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302177.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
    ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG
    CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC
    AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG
    GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC
    GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG
    TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG
    CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT
    TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA
    ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG
    GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG
    CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA
    GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG
    GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG
    AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC
    AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT
    CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT
    GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG
    CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG
    TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA
    ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC
    CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT
    GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC
    ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA
    GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG
    CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG TATCTCCTCG CCTCCTACGG
    AGAACAAGTC GCCCCCGTAT GCGGGCTGTC CCGCTCGCTT CAGGAGGTAT ACCTGAGACT
    GATGCCCACC TGCCTTCCTT ACAGACGGAA GTGTGGAGCT GGGGACAAGG CCAGGATGGA
    CAACTTGGAC ATGGAGACCT CCTTCCCAGA CTGCAGCCTG ATTGTGTAAA GAGTCTAAAT
    GGTAAGGAGG TGCTGAAAGT GGCTGCAGGG GCTCATCACT CTCTCGCTCT GACAGCACAG
    TCACAGGTTT TCTCCTGGGG TTGTAACACT TCTGGACAGC TGGGACACAT GGAATCGCCC
    ACCACCTTCC CTCAATTGAC CAAGCTGTCA GATGGGATTC GTGTGTGGGA TATTGGGGCA
    GGTCTGCAGC ACACTCTCTT ATTGGCTGAC GGAGACTGTA TTCAGCCAAT CCTGTACTAC
    AGCGGACAGC AGGTGAAGGA AGTGCCGGAG CCAACAGAGG AAGAGGAGCA GACTGCAGAA
    TACACGCAAC AACCTGTGCT GCTGCCCTTC TGTATGAACT TGGGCTATGT AAGCAGTGTG
    TATGCAGGTG GACGGACGTG TGCAGCGCTG ACGGATTGTA ATGTCATGGG TTTTATTGCT
    AGTCTGCATG AGCTGGCGGC TGCAGAGAGA AAATATTACT GCAAACTCAG CAATATCAAG
    AGTCTTCTGC TCCAGCCATT GCTCAAGCTG GATTCTCTAA GCTCTGCCCT GGGTCAGTCA
    TCTGCAGGGC TGTTGCAGAA TCTGATTGGT CGAATGAGTC GACTGTCTCA GCTTACAGGT
    CAAAACTCGG CCTCTCTGAC CTCGTTCCTG CAGCGTTCAC GAGACATCCG AGGTCTTGTT
    ATTCTTGAAG ATGCACATCT GTTCCTGGAC ACATATTCAG AGTACTCTTC TGCAGTTGGT
    GATCTTTTAG TCATGGGAGG ATTTCAAGCC CTTGTCAAAC CTTGCCATGA TGTGTTTGGG
    AAAGGAGCAG AGGTTGTTCA GAGGTTGTCT GAATGTTCTG AAGAAGGTTT GACCTTGGCA
    GATGTTCTGG CGCATCTCTT TTACCTGCCC ATAAAACACC TGCATGAATA TGGACGACTG
    CTGCTTAAAC TGGCTACCTG CTATGAGGTG AGTTCTGTGG ACTATCAGAA GCTGCAGGAT
    GCCTGCTCAA AGTTCGAATC CATGGCTCTT CATCTAAAGA GGAGGAGAAA GGAAGCTGAA
    TACACGCTCC ACTTCTGGAA GAGTTTTCCT GGAAAGATGA CCGACTCCCT GCGTAAACCG
    TCTCGTCGGC TGATCTGTGA AAGCAGTAAT AAGGCTTTGA CTCTTCAAAA CGCAGGACGT
    TTTTCCGTCA ACTGGTTCAT CCTCTTCAAC GACGCACTCG TTCATGCACA GGGTATGGTC
    CCCTATAAGA GTCTTTTTTC CACCCATCAC ATTTTTCCAT TGGCTACGCT GTGGGTGGAG
    CCAATTTCTG AGGAGAGCAC AGGCGTATAT GGCTTAAAAG TGACTTCGCC TGAGGAAAGC
    TTCGCTCTGC TGGCTTCATC ACCAGCTGAA AAAGCAAAGT GGCTTCGTTC TATAAACCAA
    GCGGTAGAGC AGGCGCTCAG TGGATTGGGG CATGATGGGA TACCTCCTTC TACAGGGATG
    ACGCAGAGAG CAGATCCACC CATCTCCCGG ACAGCATCCT ACACCTTCTA CAAAGACAGT
    CGGCTAAAGG ATGCCAAATA TGATGGCCGA TGGGTCTCAG GGAAACCCCA TGGGCGGGGC
    GTGGTCAAAT GGCCAGATGG ACGAATGTAC ACGGGGACAT TCAAGAACGG TTTTGAAGAT
    GGTTTTGGAG ATTACGTTGT GTCCAATAAA ACCCTGAACA GCTGTGATCA TTATCAAGGC
    CAGTGGAAAG ATGGCAAGAT GCACGGTTTT GGAACATTCA GATATGCCTC TGGTGAAGTG
    TACGAAGGCT CATTTCAAGA CAACATGCGT CATGGTCATG GGATGCTGCG TAGTGGGAAG
    CTGAATTCCA CCTCTCCCAG TGTCTTTATC GGCCAGTGGC AGTACGACAA AAAGTCTGGC
    TATGGAGTTT TTGATGACAT CACACGAGGA GAGAAGTATA TGGGCATGTG GCTGGATGAT
    CAACGGCAGG GGAACGGAGT TGTTGTTACA CAATTTGGCC TATACTATGA AGGTGCATTC
    AGCAACAACA AAATGATGGG TACTGGGGTG TTACTCTCTG AAGATGACAC TACATTTGAA
    GGAGAGTTTT TAGAAGACTG GACTCTTAAT GGAAAGGGTG TGTTGACCAT GCCGAATGGA
    GACTATATTG AAGGCTCTTT TTGTGGAGTT TGGGGAACCG GGCTAAAGAT GTCAGGTTCC
    TACTACAAAC CCAGCCTGTA CGACTCAGAC AAGGAGAAGG GTCATGCACT TAAACTGGGC
    CGGTTAGCAG TTCACTCTGA AGAGAAGTGG AAGGCTGTGT TTTTGGAGTG CTGGAACAGG
    CTCGGTTGTG ATTCCCCTGT ACAGGGGCAA ACCACGACAG CGTGGGACAA CATTGCCATT
    GCACTCACTG CTAACCTGAG ACAGCAGAGA GACAGTCCAG AGTTGCTGAG CCGCTCACAT
    CACAAAACAC TAGAGAGTCT TGAGTGCATC CCTCAACATG TGGGTCCTGT AACATTAGAG
    GTGTACCACA CGATCCGTCG GTACCTGGTG AAGGCATGTG ATACTCCTCT GCATCCTCTG
    GGTCGGCTGG TGGAGACGCT GGTTTCGGTA TACCGGATGA CGTATGTTGG TGTTGGAGCA
    AACCGCAGGT TATTACCGCA GGCCGTCAAT GAGATCACGT CCTATCTCAG CCGTATCTTC
    CAGCTCGTCA GATTTCTCTT TCCCGAACTG CCAGAAGAAG GAGGTTCACT AGCCGACCCT
    CCAAAAGAGA AGGTCGGCAC AGATGCTGAT GGAAAGCTGC TGGAGTCTCC TAAACCTGGG
    TGTGTGGTCA GCAGCTCTGC TCTCCTCCTG CCGGTGCTGT TGCCACGTCT CTATCCTCCT
    CTCTTCACGC TCTACGCTCT AGAGAAAGAG AAAGAGGACG ATGTGTACTG GGAGTGTGTG
    CTGCGACTCA ACAAACAGCC AGATCTGGCA CTGCTGGCTT TCCTCGGGGT CCAACAGAAG
    TTCTGGCCTG TAACTGTTAC AATACATGGA GAAAAACAAC AGGTTCTCTC AAGCACTAAA
    GACGCCTGTT TCGCCTCAGC AGTTGAGACT TTGCAGCAAA TAAGCACAAC GTTCACACCC
    TCAGACAAGC TTCAGGTGAT CCAGCTGACC TTCGAGGAAA TAACCCAGGA AGTGCTCGCA
    CTCCTGAAAC AGGATTTCTT GTGGTCTATG GATGACCTGT TTCCTGTCTT CCTCTTTGTT
    GTTCTGCGTG CACGGATAAG GAATCTGGGG TCAGAGGTTA GTCTCATTGA GGACCTAATG
    GATCCATGTG TACAACATGG AGAGCATGGC ATCATGTTCA CCACCCTCAA GGCGTGCTAC
    TATCAAATCC AGCATGAGAA GGTCACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009300452.1
    CDS250..5319
    Translation

    Target ORF information:

    RefSeq Version XM_009302177.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009302177.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
    ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG
    CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC
    AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG
    GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC
    GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG
    TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG
    CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT
    TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA
    ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG
    GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG
    CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA
    GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG
    GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG
    AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC
    AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT
    CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT
    GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG
    CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG
    TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA
    ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC
    CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT
    GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC
    ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA
    GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG
    CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG TATCTCCTCG CCTCCTACGG
    AGAACAAGTC GCCCCCGTAT GCGGGCTGTC CCGCTCGCTT CAGGAGGTAT ACCTGAGACT
    GATGCCCACC TGCCTTCCTT ACAGACGGAA GTGTGGAGCT GGGGACAAGG CCAGGATGGA
    CAACTTGGAC ATGGAGACCT CCTTCCCAGA CTGCAGCCTG ATTGTGTAAA GAGTCTAAAT
    GGTAAGGAGG TGCTGAAAGT GGCTGCAGGG GCTCATCACT CTCTCGCTCT GACAGCACAG
    TCACAGGTTT TCTCCTGGGG TTGTAACACT TCTGGACAGC TGGGACACAT GGAATCGCCC
    ACCACCTTCC CTCAATTGAC CAAGCTGTCA GATGGGATTC GTGTGTGGGA TATTGGGGCA
    GGTCTGCAGC ACACTCTCTT ATTGGCTGAC GGAGACTGTA TTCAGCCAAT CCTGTACTAC
    AGCGGACAGC AGGTGAAGGA AGTGCCGGAG CCAACAGAGG AAGAGGAGCA GACTGCAGAA
    TACACGCAAC AACCTGTGCT GCTGCCCTTC TGTATGAACT TGGGCTATGT AAGCAGTGTG
    TATGCAGGTG GACGGACGTG TGCAGCGCTG ACGGATTGTA ATGTCATGGG TTTTATTGCT
    AGTCTGCATG AGCTGGCGGC TGCAGAGAGA AAATATTACT GCAAACTCAG CAATATCAAG
    AGTCTTCTGC TCCAGCCATT GCTCAAGCTG GATTCTCTAA GCTCTGCCCT GGGTCAGTCA
    TCTGCAGGGC TGTTGCAGAA TCTGATTGGT CGAATGAGTC GACTGTCTCA GCTTACAGGT
    CAAAACTCGG CCTCTCTGAC CTCGTTCCTG CAGCGTTCAC GAGACATCCG AGGTCTTGTT
    ATTCTTGAAG ATGCACATCT GTTCCTGGAC ACATATTCAG AGTACTCTTC TGCAGTTGGT
    GATCTTTTAG TCATGGGAGG ATTTCAAGCC CTTGTCAAAC CTTGCCATGA TGTGTTTGGG
    AAAGGAGCAG AGGTTGTTCA GAGGTTGTCT GAATGTTCTG AAGAAGGTTT GACCTTGGCA
    GATGTTCTGG CGCATCTCTT TTACCTGCCC ATAAAACACC TGCATGAATA TGGACGACTG
    CTGCTTAAAC TGGCTACCTG CTATGAGGTG AGTTCTGTGG ACTATCAGAA GCTGCAGGAT
    GCCTGCTCAA AGTTCGAATC CATGGCTCTT CATCTAAAGA GGAGGAGAAA GGAAGCTGAA
    TACACGCTCC ACTTCTGGAA GAGTTTTCCT GGAAAGATGA CCGACTCCCT GCGTAAACCG
    TCTCGTCGGC TGATCTGTGA AAGCAGTAAT AAGGCTTTGA CTCTTCAAAA CGCAGGACGT
    TTTTCCGTCA ACTGGTTCAT CCTCTTCAAC GACGCACTCG TTCATGCACA GGGTATGGTC
    CCCTATAAGA GTCTTTTTTC CACCCATCAC ATTTTTCCAT TGGCTACGCT GTGGGTGGAG
    CCAATTTCTG AGGAGAGCAC AGGCGTATAT GGCTTAAAAG TGACTTCGCC TGAGGAAAGC
    TTCGCTCTGC TGGCTTCATC ACCAGCTGAA AAAGCAAAGT GGCTTCGTTC TATAAACCAA
    GCGGTAGAGC AGGCGCTCAG TGGATTGGGG CATGATGGGA TACCTCCTTC TACAGGGATG
    ACGCAGAGAG CAGATCCACC CATCTCCCGG ACAGCATCCT ACACCTTCTA CAAAGACAGT
    CGGCTAAAGG ATGCCAAATA TGATGGCCGA TGGGTCTCAG GGAAACCCCA TGGGCGGGGC
    GTGGTCAAAT GGCCAGATGG ACGAATGTAC ACGGGGACAT TCAAGAACGG TTTTGAAGAT
    GGTTTTGGAG ATTACGTTGT GTCCAATAAA ACCCTGAACA GCTGTGATCA TTATCAAGGC
    CAGTGGAAAG ATGGCAAGAT GCACGGTTTT GGAACATTCA GATATGCCTC TGGTGAAGTG
    TACGAAGGCT CATTTCAAGA CAACATGCGT CATGGTCATG GGATGCTGCG TAGTGGGAAG
    CTGAATTCCA CCTCTCCCAG TGTCTTTATC GGCCAGTGGC AGTACGACAA AAAGTCTGGC
    TATGGAGTTT TTGATGACAT CACACGAGGA GAGAAGTATA TGGGCATGTG GCTGGATGAT
    CAACGGCAGG GGAACGGAGT TGTTGTTACA CAATTTGGCC TATACTATGA AGGTGCATTC
    AGCAACAACA AAATGATGGG TACTGGGGTG TTACTCTCTG AAGATGACAC TACATTTGAA
    GGAGAGTTTT TAGAAGACTG GACTCTTAAT GGAAAGGGTG TGTTGACCAT GCCGAATGGA
    GACTATATTG AAGGCTCTTT TTGTGGAGTT TGGGGAACCG GGCTAAAGAT GTCAGGTTCC
    TACTACAAAC CCAGCCTGTA CGACTCAGAC AAGGAGAAGG GTCATGCACT TAAACTGGGC
    CGGTTAGCAG TTCACTCTGA AGAGAAGTGG AAGGCTGTGT TTTTGGAGTG CTGGAACAGG
    CTCGGTTGTG ATTCCCCTGT ACAGGGGCAA ACCACGACAG CGTGGGACAA CATTGCCATT
    GCACTCACTG CTAACCTGAG ACAGCAGAGA GACAGTCCAG AGTTGCTGAG CCGCTCACAT
    CACAAAACAC TAGAGAGTCT TGAGTGCATC CCTCAACATG TGGGTCCTGT AACATTAGAG
    GTGTACCACA CGATCCGTCG GTACCTGGTG AAGGCATGTG ATACTCCTCT GCATCCTCTG
    GGTCGGCTGG TGGAGACGCT GGTTTCGGTA TACCGGATGA CGTATGTTGG TGTTGGAGCA
    AACCGCAGGT TATTACCGCA GGCCGTCAAT GAGATCACGT CCTATCTCAG CCGTATCTTC
    CAGCTCGTCA GATTTCTCTT TCCCGAACTG CCAGAAGAAG GAGGTTCACT AGCCGACCCT
    CCAAAAGAGA AGGTCGGCAC AGATGCTGAT GGAAAGCTGC TGGAGTCTCC TAAACCTGGG
    TGTGTGGTCA GCAGCTCTGC TCTCCTCCTG CCGGTGCTGT TGCCACGTCT CTATCCTCCT
    CTCTTCACGC TCTACGCTCT AGAGAAAGAG AAAGAGGACG ATGTGTACTG GGAGTGTGTG
    CTGCGACTCA ACAAACAGCC AGATCTGGCA CTGCTGGCTT TCCTCGGGGT CCAACAGAAG
    TTCTGGCCTG TAACTGTTAC AATACATGGA GAAAAACAAC AGGTTCTCTC AAGCACTAAA
    GACGCCTGTT TCGCCTCAGC AGTTGAGACT TTGCAGCAAA TAAGCACAAC GTTCACACCC
    TCAGACAAGC TTCAGGTGAT CCAGCTGACC TTCGAGGAAA TAACCCAGGA AGTGCTCGCA
    CTCCTGAAAC AGGATTTCTT GTGGTCTATG GATGACCTGT TTCCTGTCTT CCTCTTTGTT
    GTTCTGCGTG CACGGATAAG GAATCTGGGG TCAGAGGTTA GTCTCATTGA GGACCTAATG
    GATCCATGTG TACAACATGG AGAGCATGGC ATCATGTTCA CCACCCTCAA GGCGTGCTAC
    TATCAAATCC AGCATGAGAA GGTCACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49119
    Clone ID Related Accession (Same CDS sequence) XM_009302177.2 , XM_009302177.3
    Accession Version XM_009302177.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product alsin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009302177.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
    ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG
    CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC
    AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG
    GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC
    GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG
    TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG
    CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT
    TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA
    ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG
    GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG
    CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA
    GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG
    GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG
    AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC
    AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT
    CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT
    GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG
    CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG
    TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA
    ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC
    CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT
    GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC
    ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA
    GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG
    CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG TATCTCCTCG CCTCCTACGG
    AGAACAAGTC GCCCCCGTAT GCGGGCTGTC CCGCTCGCTT CAGGAGGTAT ACCTGAGACT
    GATGCCCACC TGCCTTCCTT ACAGACGGAA GTGTGGAGCT GGGGACAAGG CCAGGATGGA
    CAACTTGGAC ATGGAGACCT CCTTCCCAGA CTGCAGCCTG ATTGTGTAAA GAGTCTAAAT
    GGTAAGGAGG TGCTGAAAGT GGCTGCAGGG GCTCATCACT CTCTCGCTCT GACAGCACAG
    TCACAGGTTT TCTCCTGGGG TTGTAACACT TCTGGACAGC TGGGACACAT GGAATCGCCC
    ACCACCTTCC CTCAATTGAC CAAGCTGTCA GATGGGATTC GTGTGTGGGA TATTGGGGCA
    GGTCTGCAGC ACACTCTCTT ATTGGCTGAC GGAGACTGTA TTCAGCCAAT CCTGTACTAC
    AGCGGACAGC AGGTGAAGGA AGTGCCGGAG CCAACAGAGG AAGAGGAGCA GACTGCAGAA
    TACACGCAAC AACCTGTGCT GCTGCCCTTC TGTATGAACT TGGGCTATGT AAGCAGTGTG
    TATGCAGGTG GACGGACGTG TGCAGCGCTG ACGGATTGTA ATGTCATGGG TTTTATTGCT
    AGTCTGCATG AGCTGGCGGC TGCAGAGAGA AAATATTACT GCAAACTCAG CAATATCAAG
    AGTCTTCTGC TCCAGCCATT GCTCAAGCTG GATTCTCTAA GCTCTGCCCT GGGTCAGTCA
    TCTGCAGGGC TGTTGCAGAA TCTGATTGGT CGAATGAGTC GACTGTCTCA GCTTACAGGT
    CAAAACTCGG CCTCTCTGAC CTCGTTCCTG CAGCGTTCAC GAGACATCCG AGGTCTTGTT
    ATTCTTGAAG ATGCACATCT GTTCCTGGAC ACATATTCAG AGTACTCTTC TGCAGTTGGT
    GATCTTTTAG TCATGGGAGG ATTTCAAGCC CTTGTCAAAC CTTGCCATGA TGTGTTTGGG
    AAAGGAGCAG AGGTTGTTCA GAGGTTGTCT GAATGTTCTG AAGAAGGTTT GACCTTGGCA
    GATGTTCTGG CGCATCTCTT TTACCTGCCC ATAAAACACC TGCATGAATA TGGACGACTG
    CTGCTTAAAC TGGCTACCTG CTATGAGGTG AGTTCTGTGG ACTATCAGAA GCTGCAGGAT
    GCCTGCTCAA AGTTCGAATC CATGGCTCTT CATCTAAAGA GGAGGAGAAA GGAAGCTGAA
    TACACGCTCC ACTTCTGGAA GAGTTTTCCT GGAAAGATGA CCGACTCCCT GCGTAAACCG
    TCTCGTCGGC TGATCTGTGA AAGCAGTAAT AAGGCTTTGA CTCTTCAAAA CGCAGGACGT
    TTTTCCGTCA ACTGGTTCAT CCTCTTCAAC GACGCACTCG TTCATGCACA GGGTATGGTC
    CCCTATAAGA GTCTTTTTTC CACCCATCAC ATTTTTCCAT TGGCTACGCT GTGGGTGGAG
    CCAATTTCTG AGGAGAGCAC AGGCGTATAT GGCTTAAAAG TGACTTCGCC TGAGGAAAGC
    TTCGCTCTGC TGGCTTCATC ACCAGCTGAA AAAGCAAAGT GGCTTCGTTC TATAAACCAA
    GCGGTAGAGC AGGCGCTCAG TGGATTGGGG CATGATGGGA TACCTCCTTC TACAGGGATG
    ACGCAGAGAG CAGATCCACC CATCTCCCGG ACAGCATCCT ACACCTTCTA CAAAGACAGT
    CGGCTAAAGG ATGCCAAATA TGATGGCCGA TGGGTCTCAG GGAAACCCCA TGGGCGGGGC
    GTGGTCAAAT GGCCAGATGG ACGAATGTAC ACGGGGACAT TCAAGAACGG TTTTGAAGAT
    GGTTTTGGAG ATTACGTTGT GTCCAATAAA ACCCTGAACA GCTGTGATCA TTATCAAGGC
    CAGTGGAAAG ATGGCAAGAT GCACGGTTTT GGAACATTCA GATATGCCTC TGGTGAAGTG
    TACGAAGGCT CATTTCAAGA CAACATGCGT CATGGTCATG GGATGCTGCG TAGTGGGAAG
    CTGAATTCCA CCTCTCCCAG TGTCTTTATC GGCCAGTGGC AGTACGACAA AAAGTCTGGC
    TATGGAGTTT TTGATGACAT CACACGAGGA GAGAAGTATA TGGGCATGTG GCTGGATGAT
    CAACGGCAGG GGAACGGAGT TGTTGTTACA CAATTTGGCC TATACTATGA AGGTGCATTC
    AGCAACAACA AAATGATGGG TACTGGGGTG TTACTCTCTG AAGATGACAC TACATTTGAA
    GGAGAGTTTT TAGAAGACTG GACTCTTAAT GGAAAGGGTG TGTTGACCAT GCCGAATGGA
    GACTATATTG AAGGCTCTTT TTGTGGAGTT TGGGGAACCG GGCTAAAGAT GTCAGGTTCC
    TACTACAAAC CCAGCCTGTA CGACTCAGAC AAGGAGAAGG GTCATGCACT TAAACTGGGC
    CGGTTAGCAG TTCACTCTGA AGAGAAGTGG AAGGCTGTGT TTTTGGAGTG CTGGAACAGG
    CTCGGTTGTG ATTCCCCTGT ACAGGGGCAA ACCACGACAG CGTGGGACAA CATTGCCATT
    GCACTCACTG CTAACCTGAG ACAGCAGAGA GACAGTCCAG AGTTGCTGAG CCGCTCACAT
    CACAAAACAC TAGAGAGTCT TGAGTGCATC CCTCAACATG TGGGTCCTGT AACATTAGAG
    GTGTACCACA CGATCCGTCG GTACCTGGTG AAGGCATGTG ATACTCCTCT GCATCCTCTG
    GGTCGGCTGG TGGAGACGCT GGTTTCGGTA TACCGGATGA CGTATGTTGG TGTTGGAGCA
    AACCGCAGGT TATTACCGCA GGCCGTCAAT GAGATCACGT CCTATCTCAG CCGTATCTTC
    CAGCTCGTCA GATTTCTCTT TCCCGAACTG CCAGAAGAAG GAGGTTCACT AGCCGACCCT
    CCAAAAGAGA AGGTCGGCAC AGATGCTGAT GGAAAGCTGC TGGAGTCTCC TAAACCTGGG
    TGTGTGGTCA GCAGCTCTGC TCTCCTCCTG CCGGTGCTGT TGCCACGTCT CTATCCTCCT
    CTCTTCACGC TCTACGCTCT AGAGAAAGAG AAAGAGGACG ATGTGTACTG GGAGTGTGTG
    CTGCGACTCA ACAAACAGCC AGATCTGGCA CTGCTGGCTT TCCTCGGGGT CCAACAGAAG
    TTCTGGCCTG TAACTGTTAC AATACATGGA GAAAAACAAC AGGTTCTCTC AAGCACTAAA
    GACGCCTGTT TCGCCTCAGC AGTTGAGACT TTGCAGCAAA TAAGCACAAC GTTCACACCC
    TCAGACAAGC TTCAGGTGAT CCAGCTGACC TTCGAGGAAA TAACCCAGGA AGTGCTCGCA
    CTCCTGAAAC AGGATTTCTT GTGGTCTATG GATGACCTGT TTCCTGTCTT CCTCTTTGTT
    GTTCTGCGTG CACGGATAAG GAATCTGGGG TCAGAGGTTA GTCTCATTGA GGACCTAATG
    GATCCATGTG TACAACATGG AGAGCATGGC ATCATGTTCA CCACCCTCAA GGCGTGCTAC
    TATCAAATCC AGCATGAGAA GGTCACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009300452.1
    CDS270..5339
    Translation

    Target ORF information:

    RefSeq Version XM_009302177.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009302177.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
    ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG
    CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC
    AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG
    GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC
    GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG
    TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG
    CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT
    TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA
    ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG
    GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG
    CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA
    GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG
    GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG
    AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC
    AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT
    CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT
    GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG
    CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG
    TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA
    ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC
    CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT
    GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC
    ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA
    GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG
    CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG TATCTCCTCG CCTCCTACGG
    AGAACAAGTC GCCCCCGTAT GCGGGCTGTC CCGCTCGCTT CAGGAGGTAT ACCTGAGACT
    GATGCCCACC TGCCTTCCTT ACAGACGGAA GTGTGGAGCT GGGGACAAGG CCAGGATGGA
    CAACTTGGAC ATGGAGACCT CCTTCCCAGA CTGCAGCCTG ATTGTGTAAA GAGTCTAAAT
    GGTAAGGAGG TGCTGAAAGT GGCTGCAGGG GCTCATCACT CTCTCGCTCT GACAGCACAG
    TCACAGGTTT TCTCCTGGGG TTGTAACACT TCTGGACAGC TGGGACACAT GGAATCGCCC
    ACCACCTTCC CTCAATTGAC CAAGCTGTCA GATGGGATTC GTGTGTGGGA TATTGGGGCA
    GGTCTGCAGC ACACTCTCTT ATTGGCTGAC GGAGACTGTA TTCAGCCAAT CCTGTACTAC
    AGCGGACAGC AGGTGAAGGA AGTGCCGGAG CCAACAGAGG AAGAGGAGCA GACTGCAGAA
    TACACGCAAC AACCTGTGCT GCTGCCCTTC TGTATGAACT TGGGCTATGT AAGCAGTGTG
    TATGCAGGTG GACGGACGTG TGCAGCGCTG ACGGATTGTA ATGTCATGGG TTTTATTGCT
    AGTCTGCATG AGCTGGCGGC TGCAGAGAGA AAATATTACT GCAAACTCAG CAATATCAAG
    AGTCTTCTGC TCCAGCCATT GCTCAAGCTG GATTCTCTAA GCTCTGCCCT GGGTCAGTCA
    TCTGCAGGGC TGTTGCAGAA TCTGATTGGT CGAATGAGTC GACTGTCTCA GCTTACAGGT
    CAAAACTCGG CCTCTCTGAC CTCGTTCCTG CAGCGTTCAC GAGACATCCG AGGTCTTGTT
    ATTCTTGAAG ATGCACATCT GTTCCTGGAC ACATATTCAG AGTACTCTTC TGCAGTTGGT
    GATCTTTTAG TCATGGGAGG ATTTCAAGCC CTTGTCAAAC CTTGCCATGA TGTGTTTGGG
    AAAGGAGCAG AGGTTGTTCA GAGGTTGTCT GAATGTTCTG AAGAAGGTTT GACCTTGGCA
    GATGTTCTGG CGCATCTCTT TTACCTGCCC ATAAAACACC TGCATGAATA TGGACGACTG
    CTGCTTAAAC TGGCTACCTG CTATGAGGTG AGTTCTGTGG ACTATCAGAA GCTGCAGGAT
    GCCTGCTCAA AGTTCGAATC CATGGCTCTT CATCTAAAGA GGAGGAGAAA GGAAGCTGAA
    TACACGCTCC ACTTCTGGAA GAGTTTTCCT GGAAAGATGA CCGACTCCCT GCGTAAACCG
    TCTCGTCGGC TGATCTGTGA AAGCAGTAAT AAGGCTTTGA CTCTTCAAAA CGCAGGACGT
    TTTTCCGTCA ACTGGTTCAT CCTCTTCAAC GACGCACTCG TTCATGCACA GGGTATGGTC
    CCCTATAAGA GTCTTTTTTC CACCCATCAC ATTTTTCCAT TGGCTACGCT GTGGGTGGAG
    CCAATTTCTG AGGAGAGCAC AGGCGTATAT GGCTTAAAAG TGACTTCGCC TGAGGAAAGC
    TTCGCTCTGC TGGCTTCATC ACCAGCTGAA AAAGCAAAGT GGCTTCGTTC TATAAACCAA
    GCGGTAGAGC AGGCGCTCAG TGGATTGGGG CATGATGGGA TACCTCCTTC TACAGGGATG
    ACGCAGAGAG CAGATCCACC CATCTCCCGG ACAGCATCCT ACACCTTCTA CAAAGACAGT
    CGGCTAAAGG ATGCCAAATA TGATGGCCGA TGGGTCTCAG GGAAACCCCA TGGGCGGGGC
    GTGGTCAAAT GGCCAGATGG ACGAATGTAC ACGGGGACAT TCAAGAACGG TTTTGAAGAT
    GGTTTTGGAG ATTACGTTGT GTCCAATAAA ACCCTGAACA GCTGTGATCA TTATCAAGGC
    CAGTGGAAAG ATGGCAAGAT GCACGGTTTT GGAACATTCA GATATGCCTC TGGTGAAGTG
    TACGAAGGCT CATTTCAAGA CAACATGCGT CATGGTCATG GGATGCTGCG TAGTGGGAAG
    CTGAATTCCA CCTCTCCCAG TGTCTTTATC GGCCAGTGGC AGTACGACAA AAAGTCTGGC
    TATGGAGTTT TTGATGACAT CACACGAGGA GAGAAGTATA TGGGCATGTG GCTGGATGAT
    CAACGGCAGG GGAACGGAGT TGTTGTTACA CAATTTGGCC TATACTATGA AGGTGCATTC
    AGCAACAACA AAATGATGGG TACTGGGGTG TTACTCTCTG AAGATGACAC TACATTTGAA
    GGAGAGTTTT TAGAAGACTG GACTCTTAAT GGAAAGGGTG TGTTGACCAT GCCGAATGGA
    GACTATATTG AAGGCTCTTT TTGTGGAGTT TGGGGAACCG GGCTAAAGAT GTCAGGTTCC
    TACTACAAAC CCAGCCTGTA CGACTCAGAC AAGGAGAAGG GTCATGCACT TAAACTGGGC
    CGGTTAGCAG TTCACTCTGA AGAGAAGTGG AAGGCTGTGT TTTTGGAGTG CTGGAACAGG
    CTCGGTTGTG ATTCCCCTGT ACAGGGGCAA ACCACGACAG CGTGGGACAA CATTGCCATT
    GCACTCACTG CTAACCTGAG ACAGCAGAGA GACAGTCCAG AGTTGCTGAG CCGCTCACAT
    CACAAAACAC TAGAGAGTCT TGAGTGCATC CCTCAACATG TGGGTCCTGT AACATTAGAG
    GTGTACCACA CGATCCGTCG GTACCTGGTG AAGGCATGTG ATACTCCTCT GCATCCTCTG
    GGTCGGCTGG TGGAGACGCT GGTTTCGGTA TACCGGATGA CGTATGTTGG TGTTGGAGCA
    AACCGCAGGT TATTACCGCA GGCCGTCAAT GAGATCACGT CCTATCTCAG CCGTATCTTC
    CAGCTCGTCA GATTTCTCTT TCCCGAACTG CCAGAAGAAG GAGGTTCACT AGCCGACCCT
    CCAAAAGAGA AGGTCGGCAC AGATGCTGAT GGAAAGCTGC TGGAGTCTCC TAAACCTGGG
    TGTGTGGTCA GCAGCTCTGC TCTCCTCCTG CCGGTGCTGT TGCCACGTCT CTATCCTCCT
    CTCTTCACGC TCTACGCTCT AGAGAAAGAG AAAGAGGACG ATGTGTACTG GGAGTGTGTG
    CTGCGACTCA ACAAACAGCC AGATCTGGCA CTGCTGGCTT TCCTCGGGGT CCAACAGAAG
    TTCTGGCCTG TAACTGTTAC AATACATGGA GAAAAACAAC AGGTTCTCTC AAGCACTAAA
    GACGCCTGTT TCGCCTCAGC AGTTGAGACT TTGCAGCAAA TAAGCACAAC GTTCACACCC
    TCAGACAAGC TTCAGGTGAT CCAGCTGACC TTCGAGGAAA TAACCCAGGA AGTGCTCGCA
    CTCCTGAAAC AGGATTTCTT GTGGTCTATG GATGACCTGT TTCCTGTCTT CCTCTTTGTT
    GTTCTGCGTG CACGGATAAG GAATCTGGGG TCAGAGGTTA GTCTCATTGA GGACCTAATG
    GATCCATGTG TACAACATGG AGAGCATGGC ATCATGTTCA CCACCCTCAA GGCGTGCTAC
    TATCAAATCC AGCATGAGAA GGTCACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.