LOC100148510 gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_021476448.1	XP_021332123.1	uncharacterized protein LOC100148510 isoform X1
XM_021476449.1	XP_021332124.1	uncharacterized protein LOC100148510 isoform X2
XM_021476450.1	XP_021332125.1	uncharacterized protein LOC100148510 isoform X2
XM_021476452.1	XP_021332127.1	uncharacterized protein LOC100148510 isoform X4
XM_005165213.3	XP_005165270.1	uncharacterized protein LOC100148510 isoform X1
XM_001921861.6	XP_001921896.3	uncharacterized protein LOC100148510 isoform X4
XM_001921861.7	XP_001921896.3	uncharacterized protein LOC100148510 isoform X1
XM_005165214.3	XP_005165271.1	uncharacterized protein LOC100148510 isoform X3
XM_009301400.2	XP_009299675.1	uncharacterized protein LOC100148510 isoform X5
XM_009301401.2	XP_009299676.1	uncharacterized protein LOC100148510 isoform X5
XM_009301399.2	XP_009299674.1	uncharacterized protein LOC100148510 isoform X2
XM_009301400.3	XP_009299675.1	uncharacterized protein LOC100148510 isoform X2
XM_009301401.3	XP_009299676.1	uncharacterized protein LOC100148510 isoform X2
XM_021471584.1	XP_021327259.1	uncharacterized protein LOC100148510 isoform X4
XM_021476451.1	XP_021332126.1	uncharacterized protein LOC100148510 isoform X3
XM_021471583.1	XP_021327258.1	uncharacterized protein LOC100148510 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa10350	XM_005165213.3 Latest version!	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
ODa10351	XM_005165214.3 Latest version!	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa10352	XM_001921861.7 Latest version!	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa10352	XM_021476448.1 Latest version!	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa10353	XM_009301400.3 Latest version!	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa10353	XM_021476449.1 Latest version!	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa10353	XM_009301401.3 Latest version!	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa10353	XM_021476450.1 Latest version!	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa142778	XM_021471583.1 Latest version!	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa142778	XM_021476451.1 Latest version!	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa142779	XM_021476452.1 Latest version!	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa142779	XM_021471584.1 Latest version!	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa48648	XM_009301399.2 Latest version!	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
View Old Accession Versions >>
ODa10352	XM_001921861.6	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa10353	XM_009301400.2	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa10353	XM_009301401.2	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
Hide Old Accession Versions >>

LOC100148510 ( XM_005165213.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165270.1 Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10350
Clone ID Related Accession (Same CDS sequence)	XM_005165213.3
Accession Version	XM_005165213.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein LOC100148510 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165213.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCATGC ACAACTCAAA AATAAGCAAA TATGACGCAT CACAATGTAT GAAAAAACAA 
CAATTAAAAA AAATCTTGCC ATCGCCACTT ATAAATGCTT GGATTTACGT GCTTGAGGTT 
ACCATAGAAA TTATGGACTT GGGAGTGGGA GTTAACATTG TTGAAACAGC AGCTCTGGGG 
AGACCCTTCA GGCTGGGGAT GCTGTACGAC TGCAGAAAAG ATGCATTTGT ACCAGGAATC 
ACACTGTGGG ATGAAGAGCA ACTGCAGCAG AGCATAAAAA TCCATCCTCA AATTAAAACA 
GATTTCTGTG TTACAAATTC AGACTTTATT GAGGCAAAAT GCCAATTACT GAACATTAAT 
GATTGTCTAA AACTGAGTTT TTTAAGTGGA CTCATCAATG TTAGTGGAGC AGCCAAGTAT 
CTCAGTGACA CCAAGAAATC CTTCAAACAG CAGAGACTGA CCCTACATTA TCATTCAACC 
ACCAAACGTG AAGAACTGAC TGTGAACCAC TTGCCATCTG AAAATATAAC CCACTATGAA 
GTGTTTGAGA ATGATGCAGC GACACATGTG GTGACAGCTG TGCTGTATGG GGCCAATGCT 
TGCTTTGTTT TTGACAGAGA AGCTTCATTA GTTGAAGACC AAAGCACTGT AGACAGAGAC 
GTAAAGGCTG CCTTAGAAAA ACTAAAAGGG ATTTCAGAAA GTACACAGAT TGATCTGAGC 
TTGGATGACA ATCAAAAGAC TGTTTTTGAG AAATTCAGCT GCACATTTTA TGGTGATTTC 
CAGTTAAAAT CTAATCCAGC CTCTTTTGAA GAAGCTCTGA GGGTTTTTGC TGATCTTCCT 
AAACTGCTGG GAGAAACCAA GGAGTGTGCA GTTCCACTGA GAGTGTGGCT TTATCCACTG 
GACAAACTGC ATTCACATTA TGTGAAACTT CAACAAGAAA TCAGCATAGA TCTAATCAGA 
AATGTTGAAT CAGTATTTGA GAGCTTAAGG ACCACTGAAA TGAAATGCAG TGATCTTCTG 
AATGATACAC CATCTTTGGC TTTTGCAGGA TTTCATGATA AAATAATGAT TATGAAGCAA 
AACTGCTGCA ACTATAAGCT GAGCTTCATG AAGAAACTTG GATCGCTATT GCCAAAAATC 
CGTAGTGATA CAGAAAATGA GACAGCCCTG ATTGAGCTCC TCCATGATCA TGAAAAATGT 
CCATTCAGAA AAAGGGATCT TGAGAAATGG GTGAAAGAAA AAGAGAATGA ATCCGTCATT 
ATTAAAACTC TTCTCAGACA ATTGACTGAT TCTGGAGCAA CAGTGGAAAA TAATCTAGAT 
GGAATTTTAA TGGATCTTAA TGTTGAAAAT GTAGTAAGCT ACACTTTCAC ATCACTTGAG 
TGTCCAGATG TGCTCCTTAC TAAACAAAAG GCTTTCCTGA ATCCATCAAC AATAGAAAAC 
AACAGTGAGG ATGCTTCTGT TTTAAGTAAA AAATCTGGGT TCACATCTGA CATCAAAACC 
ACAATGAGGA GCAACTTGAT CATCTTCAAA AACTTGATGG AATCAAAAAT ATGTAAACCA 
GCCAAATATA TTGTTGCATC GAAGGAAATA CCAAATCCAG GTTCCTGCAT TCTCATTTAC 
GAAAATGGAT CTGATGAAGC GACATGCTTT ACTCCGCCAA TAAAACCAGC TCATCCAGTC 
ACTGAACAGA TCAGTGGGGA AAGAGTGGTT TTAAAGGTGA CACCTGTGTG TCCTGCAACA 
GAAGAGCTCA GGTTACTGTA CAAAACAAAA GAAGAGAAAG ACTGGAAATC TCAGTCTGTG 
AAAAAGAGTC AAGATACAGT TATTCTGACT GATCTCAGAC CAGACACTGA GTATGAGATG 
AAATTTGCAG CCGTGGGGAA GCTGAACTAC ACCATAGACA GTGATGTGAT TCACCTCAGA 
GTTATTGACA AAAACCTCAC TGATGCCACA GAGTCCATTT TAGAAAAGCT GAGCTTGATA 
GAAGACAAGT GCAGTAAACT GATGGAGGGC AAGAGTGCAG TCACATTTAG AGCAATTCGC 
AACAAGTTTG AAGACATGAA GAGACACTGT CAGACTTACA AACAAGACAG AATTAAATCC 
ACAATCCAGT CAGTTCAAGC CTGTGAAAAA GACATCAGTG CTCTGACAGA CCTTCTACAA 
GCTCATCATG AGTCTCCATT TAACATAAGT GATATTGTGG AGTGGATCAG AGTGAAAGAA 
ACCGAATCAA AAGCAGTTGA GAAGATTCTT CAACAGCTGC TGGAAACTGG AGCTGAAGTG 
AACAATAGTT TAGATGAATA TTTGTTGGAT GTTAGTTTTG AGAATGTGGT GTGTTACACA 
TTCACTTCTC TTGATCTGCC AGACGACCTG CTTTCTGATC AAGAAGATTT CCTGAAACCT 
GAAATGATAA GGAGAAATTC TGATAAGAAA CCCAATGCTG TGTCTGAAAC TTGGCTCACA 
GGAAGTACCA GAGAGAAATT GAAAAAACAA TTAGAAATGT TTAAAGAGCT CAAATGTTCA 
TATGGCAGTC ACTCAACAAA GTTTTTGGTT ATATCAGAAG ATCAGACAAA CCATCCAGGT 
TCCTGTATTC TCCTTTATGA AAATGGATCT TATAAAGCGA AATGCTTTAC TCCGCCAATA 
AAACCAGCTG AGCCCATCAT TGAACAGGTC ATCGGGGAAA GAGTGGTTTT GAAGGTGACA 
TCTGTGTGTC CTGCAACAGA AGAGCTCAGG TTACTGTACA AAACAAAAGA AGAGAACGAC 
TGGAAATCTC AGTCTGTGAA AAAGAGTCAA GATACAGTTA TTCTGACTGA TCTCAGACCA 
GACACTGAGT ATGAGATGAA ATTTGCAGCT GTGGGGAAGC TGAACTACAC CATAGACAGT 
GATGTGATTC ACCTTAGAGT TATTGACAAG AAACTCACTG ATGCCACAGA GTCCATTTTA 
GAAAAGCTGA GCTTGATAGA AGACAAGTGC AGTAAACTGA TGGAGGACAA GAGTGCAGTC 
ACTTTTAGAG CAATTCACAA CAAGTTTGAA GACATGAGAA GACACTGTCA GATCTACAAA 
CAAGACTTAG AAATTAAAAT TACATCCACA ATGCAGTCAG TTCAAACCTG TGAAAAAGAC 
ATCAGTGCTC TGACAGACCT TCTACAAGCT CATCATGAGT CTCCATTTAA TGAAAGTGAT 
CTTATAGAGT GGATCAGAGT GAAAGAAACC GAATCAAATG CAGTTGAGAA GATTCTTCAA 
CAGCTGCTGG ACTCTGGAAC TGAGGTGAAC AATAGTTTAG ATGAATATTT GTTTGATGTT 
AGTTTTGAGA ATGTGGTGTG TTACACATTC ACTTCTCTTG ATCTGCCAGA CAACCTGCTT 
TCTAAACAAG GAGATTTCCT GAAACCTGAA ATAATGAGGA GAAATTCTAA TAAGAACCCC 
AATTCTGTGT CTGAAACTTG GCTCACAGGA AGTATCAGAG AGACTATGAA GAAGCATTTG 
GAAATATTTA ATGAGCTCAA ATGTTCATAT GGCAGTCACT CATCAACATT TTTGGTTGTA 
TATAAAAGTC ACACAAACCA TCCAGGTTCC TGCATTCTTA TTTATGAAAA TGGATCTGAT 
AAAGCGACAT GCTTCACTCC TCCAATAAAA CCAGCTAGTC CAGTCATTGA ACAGATTGGT 
GAGGATATAG TGGTTTTGAA GGCAACACCA TTATGTCCTG CAACACAAGA GCTCAGGTTA 
CTGTACAAAA AAAAAGAAGA GAATGATTGG AAATCTCAGT CTGTGAAAAC GAGTGGAGAT 
GAAGTTTTTC TGACTGGTCT CAGACCTGAC ACTGAGTATA AGATGAAATA TGTGGCCTTG 
GGGAAGCTGA ACTACACCAC AGACAGTGAT GTCATCGGAG TTACTACACG TGTGGTCAGT 
GAGAAACAGT GTGCCTTGCC TTATTCATGC AATACTAATT TCACATATTT TAACAGTGAA 
TTATTGAACA GTAACTCTAT GCAGCTAAAT GAGACTGGCA AATATCTGCA GGATAAATCT 
GAACAAAAAC TATCGATGAC AAAGGAGCCA AGGAACAACC AACCAATGTC ATTTGATTCT 
TCAGAGGAGG TCAAAGTGTG CACTGTTTTG GCTGGAAAGA CCAATAACTG TCAAACACAT 
TTTATTGCAT CACTGCGAGC CCGAATTGAG CATCTGACGG AAGTTCGTTC AGTAGACGAG 
AGTGACATTG TTTTAATTTT CTGTCCAATT GTTTCTCGAG CTGGAACTGA CATTGATGCA 
GCCATTCACA GATGCTATAC AAATGCCTCT AAGCTGACTG TTCTAGTGGT GCTTCACCAC 
ACGTTTGACC CAGAGAAAAC TGTATCAGAC AGCAGCAAAA TTGTCAACAG GGAGAATCTG 
CTCACAGTGG ACTGTCTGTT CTATGAGGAT GCAGGATTAC TGCAGTGTCA GAGGAATTCA 
GATGCATATG ACAAATCTGT CAACTGGTTA ATAGCGCAGG GCAAAAAAAC AGGTCTTAAA 
ATCTACCCAA AGAAATCACC TGATAGTCAA TCCAATGGTC GGAAATTCTG GAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165270.1
CDS	617..5173
Translation	MSMHNSKISKYDASQCMKKQQLKKILPSPLINAWIYVLEVTIEIMDLGVGVNIVETAALGRPFRLGMLYDCRKDAFVPGITLWDEEQLQQSIKIHPQIKTDFCVTNSDFIEAKCQLLNINDCLKLSFLSGLINVSGAAKYLSDTKKSFKQQRLTLHYHSTTKREELTVNHLPSENITHYEVFENDAATHVVTAVLYGANACFVFDREASLVEDQSTVDRDVKAALEKLKGISESTQIDLSLDDNQKTVFEKFSCTFYGDFQLKSNPASFEEALRVFADLPKLLGETKECAVPLRVWLYPLDKLHSHYVKLQQEISIDLIRNVESVFESLRTTEMKCSDLLNDTPSLAFAGFHDKIMIMKQNCCNYKLSFMKKLGSLLPKIRSDTENETALIELLHDHEKCPFRKRDLEKWVKEKENESVIIKTLLRQLTDSGATVENNLDGILMDLNVENVVSYTFTSLECPDVLLTKQKAFLNPSTIENNSEDASVLSKKSGFTSDIKTTMRSNLIIFKNLMESKICKPAKYIVASKEIPNPGSCILIYENGSDEATCFTPPIKPAHPVTEQISGERVVLKVTPVCPATEELRLLYKTKEEKDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKNLTDATESILEKLSLIEDKCSKLMEGKSAVTFRAIRNKFEDMKRHCQTYKQDRIKSTIQSVQACEKDISALTDLLQAHHESPFNISDIVEWIRVKETESKAVEKILQQLLETGAEVNNSLDEYLLDVSFENVVCYTFTSLDLPDDLLSDQEDFLKPEMIRRNSDKKPNAVSETWLTGSTREKLKKQLEMFKELKCSYGSHSTKFLVISEDQTNHPGSCILLYENGSYKAKCFTPPIKPAEPIIEQVIGERVVLKVTSVCPATEELRLLYKTKEENDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKKLTDATESILEKLSLIEDKCSKLMEDKSAVTFRAIHNKFEDMRRHCQIYKQDLEIKITSTMQSVQTCEKDISALTDLLQAHHESPFNESDLIEWIRVKETESNAVEKILQQLLDSGTEVNNSLDEYLFDVSFENVVCYTFTSLDLPDNLLSKQGDFLKPEIMRRNSNKNPNSVSETWLTGSIRETMKKHLEIFNELKCSYGSHSSTFLVVYKSHTNHPGSCILIYENGSDKATCFTPPIKPASPVIEQIGEDIVVLKATPLCPATQELRLLYKKKEENDWKSQSVKTSGDEVFLTGLRPDTEYKMKYVALGKLNYTTDSDVIGVTTRVVSEKQCALPYSCNTNFTYFNSELLNSNSMQLNETGKYLQDKSEQKLSMTKEPRNNQPMSFDSSEEVKVCTVLAGKTNNCQTHFIASLRARIEHLTEVRSVDESDIVLIFCPIVSRAGTDIDAAIHRCYTNASKLTVLVVLHHTFDPEKTVSDSSKIVNRENLLTVDCLFYEDAGLLQCQRNSDAYDKSVNWLIAQGKKTGLKIYPKKSPDSQSNGRKFWR

Target ORF information:

RefSeq Version	XM_005165213.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165213.3

ORF Insert Sequence:

ATGTCCATGC ACAACTCAAA AATAAGCAAA TATGACGCAT CACAATGTAT GAAAAAACAA 
CAATTAAAAA AAATCTTGCC ATCGCCACTT ATAAATGCTT GGATTTACGT GCTTGAGGTT 
ACCATAGAAA TTATGGACTT GGGAGTGGGA GTTAACATTG TTGAAACAGC AGCTCTGGGG 
AGACCCTTCA GGCTGGGGAT GCTGTACGAC TGCAGAAAAG ATGCATTTGT ACCAGGAATC 
ACACTGTGGG ATGAAGAGCA ACTGCAGCAG AGCATAAAAA TCCATCCTCA AATTAAAACA 
GATTTCTGTG TTACAAATTC AGACTTTATT GAGGCAAAAT GCCAATTACT GAACATTAAT 
GATTGTCTAA AACTGAGTTT TTTAAGTGGA CTCATCAATG TTAGTGGAGC AGCCAAGTAT 
CTCAGTGACA CCAAGAAATC CTTCAAACAG CAGAGACTGA CCCTACATTA TCATTCAACC 
ACCAAACGTG AAGAACTGAC TGTGAACCAC TTGCCATCTG AAAATATAAC CCACTATGAA 
GTGTTTGAGA ATGATGCAGC GACACATGTG GTGACAGCTG TGCTGTATGG GGCCAATGCT 
TGCTTTGTTT TTGACAGAGA AGCTTCATTA GTTGAAGACC AAAGCACTGT AGACAGAGAC 
GTAAAGGCTG CCTTAGAAAA ACTAAAAGGG ATTTCAGAAA GTACACAGAT TGATCTGAGC 
TTGGATGACA ATCAAAAGAC TGTTTTTGAG AAATTCAGCT GCACATTTTA TGGTGATTTC 
CAGTTAAAAT CTAATCCAGC CTCTTTTGAA GAAGCTCTGA GGGTTTTTGC TGATCTTCCT 
AAACTGCTGG GAGAAACCAA GGAGTGTGCA GTTCCACTGA GAGTGTGGCT TTATCCACTG 
GACAAACTGC ATTCACATTA TGTGAAACTT CAACAAGAAA TCAGCATAGA TCTAATCAGA 
AATGTTGAAT CAGTATTTGA GAGCTTAAGG ACCACTGAAA TGAAATGCAG TGATCTTCTG 
AATGATACAC CATCTTTGGC TTTTGCAGGA TTTCATGATA AAATAATGAT TATGAAGCAA 
AACTGCTGCA ACTATAAGCT GAGCTTCATG AAGAAACTTG GATCGCTATT GCCAAAAATC 
CGTAGTGATA CAGAAAATGA GACAGCCCTG ATTGAGCTCC TCCATGATCA TGAAAAATGT 
CCATTCAGAA AAAGGGATCT TGAGAAATGG GTGAAAGAAA AAGAGAATGA ATCCGTCATT 
ATTAAAACTC TTCTCAGACA ATTGACTGAT TCTGGAGCAA CAGTGGAAAA TAATCTAGAT 
GGAATTTTAA TGGATCTTAA TGTTGAAAAT GTAGTAAGCT ACACTTTCAC ATCACTTGAG 
TGTCCAGATG TGCTCCTTAC TAAACAAAAG GCTTTCCTGA ATCCATCAAC AATAGAAAAC 
AACAGTGAGG ATGCTTCTGT TTTAAGTAAA AAATCTGGGT TCACATCTGA CATCAAAACC 
ACAATGAGGA GCAACTTGAT CATCTTCAAA AACTTGATGG AATCAAAAAT ATGTAAACCA 
GCCAAATATA TTGTTGCATC GAAGGAAATA CCAAATCCAG GTTCCTGCAT TCTCATTTAC 
GAAAATGGAT CTGATGAAGC GACATGCTTT ACTCCGCCAA TAAAACCAGC TCATCCAGTC 
ACTGAACAGA TCAGTGGGGA AAGAGTGGTT TTAAAGGTGA CACCTGTGTG TCCTGCAACA 
GAAGAGCTCA GGTTACTGTA CAAAACAAAA GAAGAGAAAG ACTGGAAATC TCAGTCTGTG 
AAAAAGAGTC AAGATACAGT TATTCTGACT GATCTCAGAC CAGACACTGA GTATGAGATG 
AAATTTGCAG CCGTGGGGAA GCTGAACTAC ACCATAGACA GTGATGTGAT TCACCTCAGA 
GTTATTGACA AAAACCTCAC TGATGCCACA GAGTCCATTT TAGAAAAGCT GAGCTTGATA 
GAAGACAAGT GCAGTAAACT GATGGAGGGC AAGAGTGCAG TCACATTTAG AGCAATTCGC 
AACAAGTTTG AAGACATGAA GAGACACTGT CAGACTTACA AACAAGACAG AATTAAATCC 
ACAATCCAGT CAGTTCAAGC CTGTGAAAAA GACATCAGTG CTCTGACAGA CCTTCTACAA 
GCTCATCATG AGTCTCCATT TAACATAAGT GATATTGTGG AGTGGATCAG AGTGAAAGAA 
ACCGAATCAA AAGCAGTTGA GAAGATTCTT CAACAGCTGC TGGAAACTGG AGCTGAAGTG 
AACAATAGTT TAGATGAATA TTTGTTGGAT GTTAGTTTTG AGAATGTGGT GTGTTACACA 
TTCACTTCTC TTGATCTGCC AGACGACCTG CTTTCTGATC AAGAAGATTT CCTGAAACCT 
GAAATGATAA GGAGAAATTC TGATAAGAAA CCCAATGCTG TGTCTGAAAC TTGGCTCACA 
GGAAGTACCA GAGAGAAATT GAAAAAACAA TTAGAAATGT TTAAAGAGCT CAAATGTTCA 
TATGGCAGTC ACTCAACAAA GTTTTTGGTT ATATCAGAAG ATCAGACAAA CCATCCAGGT 
TCCTGTATTC TCCTTTATGA AAATGGATCT TATAAAGCGA AATGCTTTAC TCCGCCAATA 
AAACCAGCTG AGCCCATCAT TGAACAGGTC ATCGGGGAAA GAGTGGTTTT GAAGGTGACA 
TCTGTGTGTC CTGCAACAGA AGAGCTCAGG TTACTGTACA AAACAAAAGA AGAGAACGAC 
TGGAAATCTC AGTCTGTGAA AAAGAGTCAA GATACAGTTA TTCTGACTGA TCTCAGACCA 
GACACTGAGT ATGAGATGAA ATTTGCAGCT GTGGGGAAGC TGAACTACAC CATAGACAGT 
GATGTGATTC ACCTTAGAGT TATTGACAAG AAACTCACTG ATGCCACAGA GTCCATTTTA 
GAAAAGCTGA GCTTGATAGA AGACAAGTGC AGTAAACTGA TGGAGGACAA GAGTGCAGTC 
ACTTTTAGAG CAATTCACAA CAAGTTTGAA GACATGAGAA GACACTGTCA GATCTACAAA 
CAAGACTTAG AAATTAAAAT TACATCCACA ATGCAGTCAG TTCAAACCTG TGAAAAAGAC 
ATCAGTGCTC TGACAGACCT TCTACAAGCT CATCATGAGT CTCCATTTAA TGAAAGTGAT 
CTTATAGAGT GGATCAGAGT GAAAGAAACC GAATCAAATG CAGTTGAGAA GATTCTTCAA 
CAGCTGCTGG ACTCTGGAAC TGAGGTGAAC AATAGTTTAG ATGAATATTT GTTTGATGTT 
AGTTTTGAGA ATGTGGTGTG TTACACATTC ACTTCTCTTG ATCTGCCAGA CAACCTGCTT 
TCTAAACAAG GAGATTTCCT GAAACCTGAA ATAATGAGGA GAAATTCTAA TAAGAACCCC 
AATTCTGTGT CTGAAACTTG GCTCACAGGA AGTATCAGAG AGACTATGAA GAAGCATTTG 
GAAATATTTA ATGAGCTCAA ATGTTCATAT GGCAGTCACT CATCAACATT TTTGGTTGTA 
TATAAAAGTC ACACAAACCA TCCAGGTTCC TGCATTCTTA TTTATGAAAA TGGATCTGAT 
AAAGCGACAT GCTTCACTCC TCCAATAAAA CCAGCTAGTC CAGTCATTGA ACAGATTGGT 
GAGGATATAG TGGTTTTGAA GGCAACACCA TTATGTCCTG CAACACAAGA GCTCAGGTTA 
CTGTACAAAA AAAAAGAAGA GAATGATTGG AAATCTCAGT CTGTGAAAAC GAGTGGAGAT 
GAAGTTTTTC TGACTGGTCT CAGACCTGAC ACTGAGTATA AGATGAAATA TGTGGCCTTG 
GGGAAGCTGA ACTACACCAC AGACAGTGAT GTCATCGGAG TTACTACACG TGTGGTCAGT 
GAGAAACAGT GTGCCTTGCC TTATTCATGC AATACTAATT TCACATATTT TAACAGTGAA 
TTATTGAACA GTAACTCTAT GCAGCTAAAT GAGACTGGCA AATATCTGCA GGATAAATCT 
GAACAAAAAC TATCGATGAC AAAGGAGCCA AGGAACAACC AACCAATGTC ATTTGATTCT 
TCAGAGGAGG TCAAAGTGTG CACTGTTTTG GCTGGAAAGA CCAATAACTG TCAAACACAT 
TTTATTGCAT CACTGCGAGC CCGAATTGAG CATCTGACGG AAGTTCGTTC AGTAGACGAG 
AGTGACATTG TTTTAATTTT CTGTCCAATT GTTTCTCGAG CTGGAACTGA CATTGATGCA 
GCCATTCACA GATGCTATAC AAATGCCTCT AAGCTGACTG TTCTAGTGGT GCTTCACCAC 
ACGTTTGACC CAGAGAAAAC TGTATCAGAC AGCAGCAAAA TTGTCAACAG GGAGAATCTG 
CTCACAGTGG ACTGTCTGTT CTATGAGGAT GCAGGATTAC TGCAGTGTCA GAGGAATTCA 
GATGCATATG ACAAATCTGT CAACTGGTTA ATAGCGCAGG GCAAAAAAAC AGGTCTTAAA 
ATCTACCCAA AGAAATCACC TGATAGTCAA TCCAATGGTC GGAAATTCTG GAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100148510 ( XM_005165214.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165271.1 Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa10351
Clone ID Related Accession (Same CDS sequence)	XM_005165214.3
Accession Version	XM_005165214.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein LOC100148510 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165214.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCATGC ACAACTCAAA AATAAGCAAA TATGACGCAT CACAATGTAT GAAAAAACAA 
CAATTAAAAA AAATCTTGCC ATCGCCACTT ATAAATGCTT GGATTTACGT GCTTGAGGTT 
ACCATAGAAA TTATGGACTT GGGAGTGGGA GTTAACATTG TTGAAACAGC AGCTCTGGGG 
AGACCCTTCA GGCTGGGGAT GCTGTACGAC TGCAGAAAAG ATGCATTTGT ACCAGGAATC 
ACACTGTGGG ATGAAGAGCA ACTGCAGCAG AGCATAAAAA TCCATCCTCA AATTAAAACA 
GATTTCTGTG TTACAAATTC AGACTTTATT GAGGCAAAAT GCCAATTACT GAACATTAAT 
GATTGTCTAA AACTGAGTTT TTTAAGTGGA CTCATCAATG TTAGTGGAGC AGCCAAGTAT 
CTCAGTGACA CCAAGAAATC CTTCAAACAG CAGAGACTGA CCCTACATTA TCATTCAACC 
ACCAAACGTG AAGAACTGAC TGTGAACCAC TTGCCATCTG AAAATATAAC CCACTATGAA 
GTGTTTGAGA ATGATGCAGC GACACATGTG GTGACAGCTG TGCTGTATGG GGCCAATGCT 
TGCTTTGTTT TTGACAGAGA AGCTTCATTA GTTGAAGACC AAAGCACTGT AGACAGAGAC 
GTAAAGGCTG CCTTAGAAAA ACTAAAAGGG ATTTCAGAAA GTACACAGAT TGATCTGAGC 
TTGGATGACA ATCAAAAGAC TGTTTTTGAG AAATTCAGCT GCACATTTTA TGGTGATTTC 
CAGTTAAAAT CTAATCCAGC CTCTTTTGAA GAAGCTCTGA GGGTTTTTGC TGATCTTCCT 
AAACTGCTGG GAGAAACCAA GGAGTGTGCA GTTCCACTGA GAGTGTGGCT TTATCCACTG 
GACAAACTGC ATTCACATTA TGTGAAACTT CAACAAGAAA TCAGCATAGA TCTAATCAGA 
AATGTTGAAT CAGTATTTGA GAGCTTAAGG ACCACTGAAA TGAAATGCAG TGATCTTCTG 
AATGATACAC CATCTTTGGC TTTTGCAGGA TTTCATGATA AAATAATGAT TATGAAGCAA 
AACTGCTGCA ACTATAAGCT GAGCTTCATG AAGAAACTTG GATCGCTATT GCCAAAAATC 
CGTAGTGATA CAGAAAATGA GACAGCCCTG ATTGAGCTCC TCCATGATCA TGAAAAATGT 
CCATTCAGAA AAAGGGATCT TGAGAAATGG GTGAAAGAAA AAGAGAATGA ATCCGTCATT 
ATTAAAACTC TTCTCAGACA ATTGACTGAT TCTGGAGCAA CAGTGGAAAA TAATCTAGAT 
GGAATTTTAA TGGATCTTAA TGTTGAAAAT GTAGTAAGCT ACACTTTCAC ATCACTTGAG 
TGTCCAGATG TGCTCCTTAC TAAACAAAAG GCTTTCCTGA ATCCATCAAC AATAGAAAAC 
AACAGTGAGG ATGCTTCTGT TTTAAGTAAA AAATCTGGGT TCACATCTGA CATCAAAACC 
ACAATGAGGA GCAACTTGAT CATCTTCAAA AACTTGATGG AATCAAAAAT ATGTAAACCA 
GCCAAATATA TTGTTGCATC GAAGGAAATA CCAAATCCAG GTTCCTGCAT TCTCATTTAC 
GAAAATGGAT CTGATGAAGC GACATGCTTT ACTCCGCCAA TAAAACCAGC TCATCCAGTC 
ACTGAACAGA TCAGTGGGGA AAGAGTGGTT TTAAAGGTGA CACCTGTGTG TCCTGCAACA 
GAAGAGCTCA GGTTACTGTA CAAAACAAAA GAAGAGAAAG ACTGGAAATC TCAGTCTGTG 
AAAAAGAGTC AAGATACAGT TATTCTGACT GATCTCAGAC CAGACACTGA GTATGAGATG 
AAATTTGCAG CCGTGGGGAA GCTGAACTAC ACCATAGACA GTGATGTGAT TCACCTCAGA 
GTTATTGACA AAAACCTCAC TGATGCCACA GAGTCCATTT TAGAAAAGCT GAGCTTGATA 
GAAGACAAGT GCAGTAAACT GATGGAGGGC AAGAGTGCAG TCACATTTAG AGCAATTCGC 
AACAAGTTTG AAGACATGAA GAGACACTGT CAGACTTACA AACAAGACAG AATTAAATCC 
ACAATCCAGT CAGTTCAAGC CTGTGAAAAA GACATCAGTG CTCTGACAGA CCTTCTACAA 
GCTCATCATG AGTCTCCATT TAACATAAGT GATATTGTGG AGTGGATCAG AGTGAAAGAA 
ACCGAATCAA AAGCAGTTGA GAAGATTCTT CAACAGCTGC TGGAAACTGG AGCTGAAGTG 
AACAATAGTT TAGATGAATA TTTGTTGGAT GTTAGTTTTG AGAATGTGGT GTGTTACACA 
TTCACTTCTC TTGATCTGCC AGACGACCTG CTTTCTGATC AAGAAGATTT CCTGAAACCT 
GAAATGATAA GGAGAAATTC TGATAAGAAA CCCAATGCTG TGTCTGAAAC TTGGCTCACA 
GGAAGTACCA GAGAGAAATT GAAAAAACAA TTAGAAATGT TTAAAGAGCT CAAATGTTCA 
TATGGCAGTC ACTCAACAAA GTTTTTGGTT ATATCAGAAG ATCAGACAAA CCATCCAGGT 
TCCTGTATTC TCCTTTATGA AAATGGATCT TATAAAGCGA AATGCTTTAC TCCGCCAATA 
AAACCAGCTG AGCCCATCAT TGAACAGGTC ATCGGGGAAA GAGTGGTTTT GAAGGTGACA 
TCTGTGTGTC CTGCAACAGA AGAGCTCAGG TTACTGTACA AAACAAAAGA AGAGAACGAC 
TGGAAATCTC AGTCTGTGAA AAAGAGTCAA GATACAGTTA TTCTGACTGA TCTCAGACCA 
GACACTGAGT ATGAGATGAA ATTTGCAGCT GTGGGGAAGC TGAACTACAC CATAGACAGT 
GATGTGATTC ACCTTAGAGT TATTGACAAG AAACTCACTG ATGCCACAGA GTCCATTTTA 
GAAAAGCTGA GCTTGATAGA AGACAAGTGC AGTAAACTGA TGGAGGACAA GAGTGCAGTC 
ACTTTTAGAG CAATTCACAA CAAGTTTGAA GACATGAGAA GACACTGTCA GATCTACAAA 
CAAGACTTAG AAATTAAAAT TACATCCACA ATGCAGTCAG TTCAAACCTG TGAAAAAGAC 
ATCAGTGCTC TGACAGACCT TCTACAAGCT CATCATGAGT CTCCATTTAA TGAAAGTGAT 
CTTATAGAGT GGATCAGAGT GAAAGAAACC GAATCAAATG CAGTTGAGAA GATTCTTCAA 
CAGCTGCTGG ACTCTGGAAC TGAGGTGAAC AATAGTTTAG ATGAATATTT GTTTGATGTT 
AGTTTTGAGA ATGTGGTGTG TTACACATTC ACTTCTCTTG ATCTGCCAGA CAACCTGCTT 
TCTAAACAAG GAGATTTCCT GAAACCTGAA ATAATGAGGA GAAATTCTAA TAAGAACCCC 
AATTCTGTGT CTGAAACTTG GCTCACAGGA AGTATCAGAG AGACTATGAA GAAGCATTTG 
GAAATATTTA ATGAGCTCAA ATGTTCATAT GGCAGTCACT CATCAACATT TTTGGTTGTA 
TATAAAAGTC ACACAAACCA TCCAGGTTCC TGCATTCTTA TTTATGAAAA TGGATCTGAT 
AAAGCGACAT GCTTCACTCC TCCAATAAAA CCAGCTAGTC CAGTCATTGA ACAGATTGGT 
GAGGATATAG TGGTTTTGAA GGCAACACCA TTATGTCCTG CAACACAAGA GCTCAGGTTA 
CTGTACAAAA AAAAAGAAGA GAATGATTGG AAATCTCAGT CTGTGAAAAC GAGTGGAGAT 
GAAGTTTTTC TGACTGGTCT CAGACCTGAC ACTGAGTATA AGATGAAATA TGTGGCCTTG 
GGGAAGCTGA ACTACACCAC AGACAGTGAT GTCATCGGAG TTACTACACG TGTGGATAAA 
TCTGAACAAA AACTATCGAT GACAAAGGAG CCAAGGAACA ACCAACCAAT GTCATTTGAT 
TCTTCAGAGG AGGTCAAAGT GTGCACTGTT TTGGCTGGAA AGACCAATAA CTGTCAAACA 
CATTTTATTG CATCACTGCG AGCCCGAATT GAGCATCTGA CGGAAGTTCG TTCAGTAGAC 
GAGAGTGACA TTGTTTTAAT TTTCTGTCCA ATTGTTTCTC GAGCTGGAAC TGACATTGAT 
GCAGCCATTC ACAGATGCTA TACAAATGCC TCTAAGCTGA CTGTTCTAGT GGTGCTTCAC 
CACACGTTTG ACCCAGAGAA AACTGTATCA GACAGCAGCA AAATTGTCAA CAGGGAGAAT 
CTGCTCACAG TGGACTGTCT GTTCTATGAG GATGCAGGAT TACTGCAGTG TCAGAGGAAT 
TCAGATGCAT ATGACAAATC TGTCAACTGG TTAATAGCGC AGGGCAAAAA AACAGGTCTT 
AAAATCTACC CAAAGAAATC ACCTGATAGT CAATCCAATG GTCGGAAATT CTGGAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165271.1
CDS	617..5056
Translation	MSMHNSKISKYDASQCMKKQQLKKILPSPLINAWIYVLEVTIEIMDLGVGVNIVETAALGRPFRLGMLYDCRKDAFVPGITLWDEEQLQQSIKIHPQIKTDFCVTNSDFIEAKCQLLNINDCLKLSFLSGLINVSGAAKYLSDTKKSFKQQRLTLHYHSTTKREELTVNHLPSENITHYEVFENDAATHVVTAVLYGANACFVFDREASLVEDQSTVDRDVKAALEKLKGISESTQIDLSLDDNQKTVFEKFSCTFYGDFQLKSNPASFEEALRVFADLPKLLGETKECAVPLRVWLYPLDKLHSHYVKLQQEISIDLIRNVESVFESLRTTEMKCSDLLNDTPSLAFAGFHDKIMIMKQNCCNYKLSFMKKLGSLLPKIRSDTENETALIELLHDHEKCPFRKRDLEKWVKEKENESVIIKTLLRQLTDSGATVENNLDGILMDLNVENVVSYTFTSLECPDVLLTKQKAFLNPSTIENNSEDASVLSKKSGFTSDIKTTMRSNLIIFKNLMESKICKPAKYIVASKEIPNPGSCILIYENGSDEATCFTPPIKPAHPVTEQISGERVVLKVTPVCPATEELRLLYKTKEEKDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKNLTDATESILEKLSLIEDKCSKLMEGKSAVTFRAIRNKFEDMKRHCQTYKQDRIKSTIQSVQACEKDISALTDLLQAHHESPFNISDIVEWIRVKETESKAVEKILQQLLETGAEVNNSLDEYLLDVSFENVVCYTFTSLDLPDDLLSDQEDFLKPEMIRRNSDKKPNAVSETWLTGSTREKLKKQLEMFKELKCSYGSHSTKFLVISEDQTNHPGSCILLYENGSYKAKCFTPPIKPAEPIIEQVIGERVVLKVTSVCPATEELRLLYKTKEENDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKKLTDATESILEKLSLIEDKCSKLMEDKSAVTFRAIHNKFEDMRRHCQIYKQDLEIKITSTMQSVQTCEKDISALTDLLQAHHESPFNESDLIEWIRVKETESNAVEKILQQLLDSGTEVNNSLDEYLFDVSFENVVCYTFTSLDLPDNLLSKQGDFLKPEIMRRNSNKNPNSVSETWLTGSIRETMKKHLEIFNELKCSYGSHSSTFLVVYKSHTNHPGSCILIYENGSDKATCFTPPIKPASPVIEQIGEDIVVLKATPLCPATQELRLLYKKKEENDWKSQSVKTSGDEVFLTGLRPDTEYKMKYVALGKLNYTTDSDVIGVTTRVDKSEQKLSMTKEPRNNQPMSFDSSEEVKVCTVLAGKTNNCQTHFIASLRARIEHLTEVRSVDESDIVLIFCPIVSRAGTDIDAAIHRCYTNASKLTVLVVLHHTFDPEKTVSDSSKIVNRENLLTVDCLFYEDAGLLQCQRNSDAYDKSVNWLIAQGKKTGLKIYPKKSPDSQSNGRKFWR

Target ORF information:

RefSeq Version	XM_005165214.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165214.3

ORF Insert Sequence:

ATGTCCATGC ACAACTCAAA AATAAGCAAA TATGACGCAT CACAATGTAT GAAAAAACAA 
CAATTAAAAA AAATCTTGCC ATCGCCACTT ATAAATGCTT GGATTTACGT GCTTGAGGTT 
ACCATAGAAA TTATGGACTT GGGAGTGGGA GTTAACATTG TTGAAACAGC AGCTCTGGGG 
AGACCCTTCA GGCTGGGGAT GCTGTACGAC TGCAGAAAAG ATGCATTTGT ACCAGGAATC 
ACACTGTGGG ATGAAGAGCA ACTGCAGCAG AGCATAAAAA TCCATCCTCA AATTAAAACA 
GATTTCTGTG TTACAAATTC AGACTTTATT GAGGCAAAAT GCCAATTACT GAACATTAAT 
GATTGTCTAA AACTGAGTTT TTTAAGTGGA CTCATCAATG TTAGTGGAGC AGCCAAGTAT 
CTCAGTGACA CCAAGAAATC CTTCAAACAG CAGAGACTGA CCCTACATTA TCATTCAACC 
ACCAAACGTG AAGAACTGAC TGTGAACCAC TTGCCATCTG AAAATATAAC CCACTATGAA 
GTGTTTGAGA ATGATGCAGC GACACATGTG GTGACAGCTG TGCTGTATGG GGCCAATGCT 
TGCTTTGTTT TTGACAGAGA AGCTTCATTA GTTGAAGACC AAAGCACTGT AGACAGAGAC 
GTAAAGGCTG CCTTAGAAAA ACTAAAAGGG ATTTCAGAAA GTACACAGAT TGATCTGAGC 
TTGGATGACA ATCAAAAGAC TGTTTTTGAG AAATTCAGCT GCACATTTTA TGGTGATTTC 
CAGTTAAAAT CTAATCCAGC CTCTTTTGAA GAAGCTCTGA GGGTTTTTGC TGATCTTCCT 
AAACTGCTGG GAGAAACCAA GGAGTGTGCA GTTCCACTGA GAGTGTGGCT TTATCCACTG 
GACAAACTGC ATTCACATTA TGTGAAACTT CAACAAGAAA TCAGCATAGA TCTAATCAGA 
AATGTTGAAT CAGTATTTGA GAGCTTAAGG ACCACTGAAA TGAAATGCAG TGATCTTCTG 
AATGATACAC CATCTTTGGC TTTTGCAGGA TTTCATGATA AAATAATGAT TATGAAGCAA 
AACTGCTGCA ACTATAAGCT GAGCTTCATG AAGAAACTTG GATCGCTATT GCCAAAAATC 
CGTAGTGATA CAGAAAATGA GACAGCCCTG ATTGAGCTCC TCCATGATCA TGAAAAATGT 
CCATTCAGAA AAAGGGATCT TGAGAAATGG GTGAAAGAAA AAGAGAATGA ATCCGTCATT 
ATTAAAACTC TTCTCAGACA ATTGACTGAT TCTGGAGCAA CAGTGGAAAA TAATCTAGAT 
GGAATTTTAA TGGATCTTAA TGTTGAAAAT GTAGTAAGCT ACACTTTCAC ATCACTTGAG 
TGTCCAGATG TGCTCCTTAC TAAACAAAAG GCTTTCCTGA ATCCATCAAC AATAGAAAAC 
AACAGTGAGG ATGCTTCTGT TTTAAGTAAA AAATCTGGGT TCACATCTGA CATCAAAACC 
ACAATGAGGA GCAACTTGAT CATCTTCAAA AACTTGATGG AATCAAAAAT ATGTAAACCA 
GCCAAATATA TTGTTGCATC GAAGGAAATA CCAAATCCAG GTTCCTGCAT TCTCATTTAC 
GAAAATGGAT CTGATGAAGC GACATGCTTT ACTCCGCCAA TAAAACCAGC TCATCCAGTC 
ACTGAACAGA TCAGTGGGGA AAGAGTGGTT TTAAAGGTGA CACCTGTGTG TCCTGCAACA 
GAAGAGCTCA GGTTACTGTA CAAAACAAAA GAAGAGAAAG ACTGGAAATC TCAGTCTGTG 
AAAAAGAGTC AAGATACAGT TATTCTGACT GATCTCAGAC CAGACACTGA GTATGAGATG 
AAATTTGCAG CCGTGGGGAA GCTGAACTAC ACCATAGACA GTGATGTGAT TCACCTCAGA 
GTTATTGACA AAAACCTCAC TGATGCCACA GAGTCCATTT TAGAAAAGCT GAGCTTGATA 
GAAGACAAGT GCAGTAAACT GATGGAGGGC AAGAGTGCAG TCACATTTAG AGCAATTCGC 
AACAAGTTTG AAGACATGAA GAGACACTGT CAGACTTACA AACAAGACAG AATTAAATCC 
ACAATCCAGT CAGTTCAAGC CTGTGAAAAA GACATCAGTG CTCTGACAGA CCTTCTACAA 
GCTCATCATG AGTCTCCATT TAACATAAGT GATATTGTGG AGTGGATCAG AGTGAAAGAA 
ACCGAATCAA AAGCAGTTGA GAAGATTCTT CAACAGCTGC TGGAAACTGG AGCTGAAGTG 
AACAATAGTT TAGATGAATA TTTGTTGGAT GTTAGTTTTG AGAATGTGGT GTGTTACACA 
TTCACTTCTC TTGATCTGCC AGACGACCTG CTTTCTGATC AAGAAGATTT CCTGAAACCT 
GAAATGATAA GGAGAAATTC TGATAAGAAA CCCAATGCTG TGTCTGAAAC TTGGCTCACA 
GGAAGTACCA GAGAGAAATT GAAAAAACAA TTAGAAATGT TTAAAGAGCT CAAATGTTCA 
TATGGCAGTC ACTCAACAAA GTTTTTGGTT ATATCAGAAG ATCAGACAAA CCATCCAGGT 
TCCTGTATTC TCCTTTATGA AAATGGATCT TATAAAGCGA AATGCTTTAC TCCGCCAATA 
AAACCAGCTG AGCCCATCAT TGAACAGGTC ATCGGGGAAA GAGTGGTTTT GAAGGTGACA 
TCTGTGTGTC CTGCAACAGA AGAGCTCAGG TTACTGTACA AAACAAAAGA AGAGAACGAC 
TGGAAATCTC AGTCTGTGAA AAAGAGTCAA GATACAGTTA TTCTGACTGA TCTCAGACCA 
GACACTGAGT ATGAGATGAA ATTTGCAGCT GTGGGGAAGC TGAACTACAC CATAGACAGT 
GATGTGATTC ACCTTAGAGT TATTGACAAG AAACTCACTG ATGCCACAGA GTCCATTTTA 
GAAAAGCTGA GCTTGATAGA AGACAAGTGC AGTAAACTGA TGGAGGACAA GAGTGCAGTC 
ACTTTTAGAG CAATTCACAA CAAGTTTGAA GACATGAGAA GACACTGTCA GATCTACAAA 
CAAGACTTAG AAATTAAAAT TACATCCACA ATGCAGTCAG TTCAAACCTG TGAAAAAGAC 
ATCAGTGCTC TGACAGACCT TCTACAAGCT CATCATGAGT CTCCATTTAA TGAAAGTGAT 
CTTATAGAGT GGATCAGAGT GAAAGAAACC GAATCAAATG CAGTTGAGAA GATTCTTCAA 
CAGCTGCTGG ACTCTGGAAC TGAGGTGAAC AATAGTTTAG ATGAATATTT GTTTGATGTT 
AGTTTTGAGA ATGTGGTGTG TTACACATTC ACTTCTCTTG ATCTGCCAGA CAACCTGCTT 
TCTAAACAAG GAGATTTCCT GAAACCTGAA ATAATGAGGA GAAATTCTAA TAAGAACCCC 
AATTCTGTGT CTGAAACTTG GCTCACAGGA AGTATCAGAG AGACTATGAA GAAGCATTTG 
GAAATATTTA ATGAGCTCAA ATGTTCATAT GGCAGTCACT CATCAACATT TTTGGTTGTA 
TATAAAAGTC ACACAAACCA TCCAGGTTCC TGCATTCTTA TTTATGAAAA TGGATCTGAT 
AAAGCGACAT GCTTCACTCC TCCAATAAAA CCAGCTAGTC CAGTCATTGA ACAGATTGGT 
GAGGATATAG TGGTTTTGAA GGCAACACCA TTATGTCCTG CAACACAAGA GCTCAGGTTA 
CTGTACAAAA AAAAAGAAGA GAATGATTGG AAATCTCAGT CTGTGAAAAC GAGTGGAGAT 
GAAGTTTTTC TGACTGGTCT CAGACCTGAC ACTGAGTATA AGATGAAATA TGTGGCCTTG 
GGGAAGCTGA ACTACACCAC AGACAGTGAT GTCATCGGAG TTACTACACG TGTGGATAAA 
TCTGAACAAA AACTATCGAT GACAAAGGAG CCAAGGAACA ACCAACCAAT GTCATTTGAT 
TCTTCAGAGG AGGTCAAAGT GTGCACTGTT TTGGCTGGAA AGACCAATAA CTGTCAAACA 
CATTTTATTG CATCACTGCG AGCCCGAATT GAGCATCTGA CGGAAGTTCG TTCAGTAGAC 
GAGAGTGACA TTGTTTTAAT TTTCTGTCCA ATTGTTTCTC GAGCTGGAAC TGACATTGAT 
GCAGCCATTC ACAGATGCTA TACAAATGCC TCTAAGCTGA CTGTTCTAGT GGTGCTTCAC 
CACACGTTTG ACCCAGAGAA AACTGTATCA GACAGCAGCA AAATTGTCAA CAGGGAGAAT 
CTGCTCACAG TGGACTGTCT GTTCTATGAG GATGCAGGAT TACTGCAGTG TCAGAGGAAT 
TCAGATGCAT ATGACAAATC TGTCAACTGG TTAATAGCGC AGGGCAAAAA AACAGGTCTT 
AAAATCTACC CAAAGAAATC ACCTGATAGT CAATCCAATG GTCGGAAATT CTGGAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100148510 ( XM_021476448.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332123.1 Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X1, mRNA.

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CloneID	ODa10352
Clone ID Related Accession (Same CDS sequence)	XM_021476448.1 , XM_001921861.6 , XM_001921861.7
Accession Version	XM_021476448.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC100148510 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAGTA TGGACTTGGG AGTGGGAGTT AACATTGTTG AAACAGCAGC TCTGGGGAGA 
CCCTTCAGGC TGGGGATGCT GTACGACTGC AGAAAAGATG CATTTGTACC AGGAATCACA 
CTGTGGGATG AAGAGCAACT GCAGCAGAGC ATAAAAATCC ATCCTCAAAT TAAAACAGAT 
TTCTGTGTTA CAAATTCAGA CTTTATTGAG GCAAAATGCC AATTACTGAA CATTAATGAT 
TGTCTAAAAC TGAGTTTTTT AAGTGGACTC ATCAATGTTA GTGGAGCAGC CAAGTATCTC 
AGTGACACCA AGAAATCCTT CAAACAGCAG AGACTGACCC TACATTATCA TTCAACCACC 
AAACGTGAAG AACTGACTGT GAACCACTTG CCATCTGAAA ATATAACCCA CTATGAAGTG 
TTTGAGAATG ATGCAGCGAC ACATGTGGTG ACAGCTGTGC TGTATGGGGC CAATGCTTGC 
TTTGTTTTTG ACAGAGAAGC TTCATTAGTT GAAGACCAAA GCACTGTAGA CAGAGACGTA 
AAGGCTGCCT TAGAAAAACT AAAAGGGATT TCAGAAAGTA CACAGATTGA TCTGAGCTTG 
GATGACAATC AAAAGACTGT TTTTGAGAAA TTCAGCTGCA CATTTTATGG TGATTTCCAG 
TTAAAATCTA ATCCAGCCTC TTTTGAAGAA GCTCTGAGGG TTTTTGCTGA TCTTCCTAAA 
CTGCTGGGAG AAACCAAGGA GTGTGCAGTT CCACTGAGAG TGTGGCTTTA TCCACTGGAC 
AAACTGCATT CACATTATGT GAAACTTCAA CAAGAAATCA GCATAGATCT AATCAGAAAT 
GTTGAATCAG TATTTGAGAG CTTAAGGACC ACTGAAATGA AATGCAGTGA TCTTCTGAAT 
GATACACCAT CTTTGGCTTT TGCAGGATTT CATGATAAAA TAATGATTAT GAAGCAAAAC 
TGCTGCAACT ATAAGCTGAG CTTCATGAAG AAACTTGGAT CGCTATTGCC AAAAATCCGT 
AGTGATACAG AAAATGAGAC AGCCCTGATT GAGCTCCTCC ATGATCATGA AAAATGTCCA 
TTCAGAAAAA GGGATCTTGA GAAATGGGTG AAAGAAAAAG AGAATGAATC CGTCATTATT 
AAAACTCTTC TCAGACAATT GACTGATTCT GGAGCAACAG TGGAAAATAA TCTAGATGGA 
ATTTTAATGG ATCTTAATGT TGAAAATGTA GTAAGCTACA CTTTCACATC ACTTGAGTGT 
CCAGATGTGC TCCTTACTAA ACAAAAGGCT TTCCTGAATC CATCAACAAT AGAAAACAAC 
AGTGAGGATG CTTCTGTTTT AAGTAAAAAA TCTGGGTTCA CATCTGACAT CAAAACCACA 
ATGAGGAGCA ACTTGATCAT CTTCAAAAAC TTGATGGAAT CAAAAATATG TAAACCAGCC 
AAATATATTG TTGCATCGAA GGAAATACCA AATCCAGGTT CCTGCATTCT CATTTACGAA 
AATGGATCTG ATGAAGCGAC ATGCTTTACT CCGCCAATAA AACCAGCTCA TCCAGTCACT 
GAACAGATCA GTGGGGAAAG AGTGGTTTTA AAGGTGACAC CTGTGTGTCC TGCAACAGAA 
GAGCTCAGGT TACTGTACAA AACAAAAGAA GAGAAAGACT GGAAATCTCA GTCTGTGAAA 
AAGAGTCAAG ATACAGTTAT TCTGACTGAT CTCAGACCAG ACACTGAGTA TGAGATGAAA 
TTTGCAGCCG TGGGGAAGCT GAACTACACC ATAGACAGTG ATGTGATTCA CCTCAGAGTT 
ATTGACAAAA ACCTCACTGA TGCCACAGAG TCCATTTTAG AAAAGCTGAG CTTGATAGAA 
GACAAGTGCA GTAAACTGAT GGAGGGCAAG AGTGCAGTCA CATTTAGAGC AATTCGCAAC 
AAGTTTGAAG ACATGAAGAG ACACTGTCAG ACTTACAAAC AAGACAGAAT TAAATCCACA 
ATCCAGTCAG TTCAAGCCTG TGAAAAAGAC ATCAGTGCTC TGACAGACCT TCTACAAGCT 
CATCATGAGT CTCCATTTAA CATAAGTGAT ATTGTGGAGT GGATCAGAGT GAAAGAAACC 
GAATCAAAAG CAGTTGAGAA GATTCTTCAA CAGCTGCTGG AAACTGGAGC TGAAGTGAAC 
AATAGTTTAG ATGAATATTT GTTGGATGTT AGTTTTGAGA ATGTGGTGTG TTACACATTC 
ACTTCTCTTG ATCTGCCAGA CGACCTGCTT TCTGATCAAG AAGATTTCCT GAAACCTGAA 
ATGATAAGGA GAAATTCTGA TAAGAAACCC AATGCTGTGT CTGAAACTTG GCTCACAGGA 
AGTACCAGAG AGAAATTGAA AAAACAATTA GAAATGTTTA AAGAGCTCAA ATGTTCATAT 
GGCAGTCACT CAACAAAGTT TTTGGTTATA TCAGAAGATC AGACAAACCA TCCAGGTTCC 
TGTATTCTCC TTTATGAAAA TGGATCTTAT AAAGCGAAAT GCTTTACTCC GCCAATAAAA 
CCAGCTGAGC CCATCATTGA ACAGGTCATC GGGGAAAGAG TGGTTTTGAA GGTGACATCT 
GTGTGTCCTG CAACAGAAGA GCTCAGGTTA CTGTACAAAA CAAAAGAAGA GAACGACTGG 
AAATCTCAGT CTGTGAAAAA GAGTCAAGAT ACAGTTATTC TGACTGATCT CAGACCAGAC 
ACTGAGTATG AGATGAAATT TGCAGCTGTG GGGAAGCTGA ACTACACCAT AGACAGTGAT 
GTGATTCACC TTAGAGTTAT TGACAAGAAA CTCACTGATG CCACAGAGTC CATTTTAGAA 
AAGCTGAGCT TGATAGAAGA CAAGTGCAGT AAACTGATGG AGGACAAGAG TGCAGTCACT 
TTTAGAGCAA TTCACAACAA GTTTGAAGAC ATGAGAAGAC ACTGTCAGAT CTACAAACAA 
GACTTAGAAA TTAAAATTAC ATCCACAATG CAGTCAGTTC AAACCTGTGA AAAAGACATC 
AGTGCTCTGA CAGACCTTCT ACAAGCTCAT CATGAGTCTC CATTTAATGA AAGTGATCTT 
ATAGAGTGGA TCAGAGTGAA AGAAACCGAA TCAAATGCAG TTGAGAAGAT TCTTCAACAG 
CTGCTGGACT CTGGAACTGA GGTGAACAAT AGTTTAGATG AATATTTGTT TGATGTTAGT 
TTTGAGAATG TGGTGTGTTA CACATTCACT TCTCTTGATC TGCCAGACAA CCTGCTTTCT 
AAACAAGGAG ATTTCCTGAA ACCTGAAATA ATGAGGAGAA ATTCTAATAA GAACCCCAAT 
TCTGTGTCTG AAACTTGGCT CACAGGAAGT ATCAGAGAGA CTATGAAGAA GCATTTGGAA 
ATATTTAATG AGCTCAAATG TTCATATGGC AGTCACTCAT CAACATTTTT GGTTGTATAT 
AAAAGTCACA CAAACCATCC AGGTTCCTGC ATTCTTATTT ATGAAAATGG ATCTGATAAA 
GCGACATGCT TCACTCCTCC AATAAAACCA GCTAGTCCAG TCATTGAACA GATTGGTGAG 
GATATAGTGG TTTTGAAGGC AACACCATTA TGTCCTGCAA CACAAGAGCT CAGGTTACTG 
TACAAAAAAA AAGAAGAGAA TGATTGGAAA TCTCAGTCTG TGAAAACGAG TGGAGATGAA 
GTTTTTCTGA CTGGTCTCAG ACCTGACACT GAGTATAAGA TGAAATATGT GGCCTTGGGG 
AAGCTGAACT ACACCACAGA CAGTGATGTC ATCGGAGTTA CTACACGTGT GGTCAGTGAG 
AAACAGTGTG CCTTGCCTTA TTCATGCAAT ACTAATTTCA CATATTTTAA CAGTGAATTA 
TTGAACAGTA ACTCTATGCA GCTAAATGAG ACTGGCAAAT ATCTGCAGGA TAAATCTGAA 
CAAAAACTAT CGATGACAAA GGAGCCAAGG AACAACCAAC CAATGTCATT TGATTCTTCA 
GAGGAGGTCA AAGTGTGCAC TGTTTTGGCT GGAAAGACCA ATAACTGTCA AACACATTTT 
ATTGCATCAC TGCGAGCCCG AATTGAGCAT CTGACGGAAG TTCGTTCAGT AGACGAGAGT 
GACATTGTTT TAATTTTCTG TCCAATTGTT TCTCGAGCTG GAACTGACAT TGATGCAGCC 
ATTCACAGAT GCTATACAAA TGCCTCTAAG CTGACTGTTC TAGTGGTGCT TCACCACACG 
TTTGACCCAG AGAAAACTGT ATCAGACAGC AGCAAAATTG TCAACAGGGA GAATCTGCTC 
ACAGTGGACT GTCTGTTCTA TGAGGATGCA GGATTACTGC AGTGTCAGAG GAATTCAGAT 
GCATATGACA AATCTGTCAA CTGGTTAATA GCGCAGGGCA AAAAAACAGG TCTTAAAATC 
TACCCAAAGA AATCACCTGA TAGTCAATCC AATGGTCGGA AATTCTGGAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332123.1
CDS	301..4734
Translation	MRSMDLGVGVNIVETAALGRPFRLGMLYDCRKDAFVPGITLWDEEQLQQSIKIHPQIKTDFCVTNSDFIEAKCQLLNINDCLKLSFLSGLINVSGAAKYLSDTKKSFKQQRLTLHYHSTTKREELTVNHLPSENITHYEVFENDAATHVVTAVLYGANACFVFDREASLVEDQSTVDRDVKAALEKLKGISESTQIDLSLDDNQKTVFEKFSCTFYGDFQLKSNPASFEEALRVFADLPKLLGETKECAVPLRVWLYPLDKLHSHYVKLQQEISIDLIRNVESVFESLRTTEMKCSDLLNDTPSLAFAGFHDKIMIMKQNCCNYKLSFMKKLGSLLPKIRSDTENETALIELLHDHEKCPFRKRDLEKWVKEKENESVIIKTLLRQLTDSGATVENNLDGILMDLNVENVVSYTFTSLECPDVLLTKQKAFLNPSTIENNSEDASVLSKKSGFTSDIKTTMRSNLIIFKNLMESKICKPAKYIVASKEIPNPGSCILIYENGSDEATCFTPPIKPAHPVTEQISGERVVLKVTPVCPATEELRLLYKTKEEKDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKNLTDATESILEKLSLIEDKCSKLMEGKSAVTFRAIRNKFEDMKRHCQTYKQDRIKSTIQSVQACEKDISALTDLLQAHHESPFNISDIVEWIRVKETESKAVEKILQQLLETGAEVNNSLDEYLLDVSFENVVCYTFTSLDLPDDLLSDQEDFLKPEMIRRNSDKKPNAVSETWLTGSTREKLKKQLEMFKELKCSYGSHSTKFLVISEDQTNHPGSCILLYENGSYKAKCFTPPIKPAEPIIEQVIGERVVLKVTSVCPATEELRLLYKTKEENDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKKLTDATESILEKLSLIEDKCSKLMEDKSAVTFRAIHNKFEDMRRHCQIYKQDLEIKITSTMQSVQTCEKDISALTDLLQAHHESPFNESDLIEWIRVKETESNAVEKILQQLLDSGTEVNNSLDEYLFDVSFENVVCYTFTSLDLPDNLLSKQGDFLKPEIMRRNSNKNPNSVSETWLTGSIRETMKKHLEIFNELKCSYGSHSSTFLVVYKSHTNHPGSCILIYENGSDKATCFTPPIKPASPVIEQIGEDIVVLKATPLCPATQELRLLYKKKEENDWKSQSVKTSGDEVFLTGLRPDTEYKMKYVALGKLNYTTDSDVIGVTTRVVSEKQCALPYSCNTNFTYFNSELLNSNSMQLNETGKYLQDKSEQKLSMTKEPRNNQPMSFDSSEEVKVCTVLAGKTNNCQTHFIASLRARIEHLTEVRSVDESDIVLIFCPIVSRAGTDIDAAIHRCYTNASKLTVLVVLHHTFDPEKTVSDSSKIVNRENLLTVDCLFYEDAGLLQCQRNSDAYDKSVNWLIAQGKKTGLKIYPKKSPDSQSNGRKFWR

Target ORF information:

RefSeq Version	XM_021476448.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476448.1

ORF Insert Sequence:

ATGCGCAGTA TGGACTTGGG AGTGGGAGTT AACATTGTTG AAACAGCAGC TCTGGGGAGA 
CCCTTCAGGC TGGGGATGCT GTACGACTGC AGAAAAGATG CATTTGTACC AGGAATCACA 
CTGTGGGATG AAGAGCAACT GCAGCAGAGC ATAAAAATCC ATCCTCAAAT TAAAACAGAT 
TTCTGTGTTA CAAATTCAGA CTTTATTGAG GCAAAATGCC AATTACTGAA CATTAATGAT 
TGTCTAAAAC TGAGTTTTTT AAGTGGACTC ATCAATGTTA GTGGAGCAGC CAAGTATCTC 
AGTGACACCA AGAAATCCTT CAAACAGCAG AGACTGACCC TACATTATCA TTCAACCACC 
AAACGTGAAG AACTGACTGT GAACCACTTG CCATCTGAAA ATATAACCCA CTATGAAGTG 
TTTGAGAATG ATGCAGCGAC ACATGTGGTG ACAGCTGTGC TGTATGGGGC CAATGCTTGC 
TTTGTTTTTG ACAGAGAAGC TTCATTAGTT GAAGACCAAA GCACTGTAGA CAGAGACGTA 
AAGGCTGCCT TAGAAAAACT AAAAGGGATT TCAGAAAGTA CACAGATTGA TCTGAGCTTG 
GATGACAATC AAAAGACTGT TTTTGAGAAA TTCAGCTGCA CATTTTATGG TGATTTCCAG 
TTAAAATCTA ATCCAGCCTC TTTTGAAGAA GCTCTGAGGG TTTTTGCTGA TCTTCCTAAA 
CTGCTGGGAG AAACCAAGGA GTGTGCAGTT CCACTGAGAG TGTGGCTTTA TCCACTGGAC 
AAACTGCATT CACATTATGT GAAACTTCAA CAAGAAATCA GCATAGATCT AATCAGAAAT 
GTTGAATCAG TATTTGAGAG CTTAAGGACC ACTGAAATGA AATGCAGTGA TCTTCTGAAT 
GATACACCAT CTTTGGCTTT TGCAGGATTT CATGATAAAA TAATGATTAT GAAGCAAAAC 
TGCTGCAACT ATAAGCTGAG CTTCATGAAG AAACTTGGAT CGCTATTGCC AAAAATCCGT 
AGTGATACAG AAAATGAGAC AGCCCTGATT GAGCTCCTCC ATGATCATGA AAAATGTCCA 
TTCAGAAAAA GGGATCTTGA GAAATGGGTG AAAGAAAAAG AGAATGAATC CGTCATTATT 
AAAACTCTTC TCAGACAATT GACTGATTCT GGAGCAACAG TGGAAAATAA TCTAGATGGA 
ATTTTAATGG ATCTTAATGT TGAAAATGTA GTAAGCTACA CTTTCACATC ACTTGAGTGT 
CCAGATGTGC TCCTTACTAA ACAAAAGGCT TTCCTGAATC CATCAACAAT AGAAAACAAC 
AGTGAGGATG CTTCTGTTTT AAGTAAAAAA TCTGGGTTCA CATCTGACAT CAAAACCACA 
ATGAGGAGCA ACTTGATCAT CTTCAAAAAC TTGATGGAAT CAAAAATATG TAAACCAGCC 
AAATATATTG TTGCATCGAA GGAAATACCA AATCCAGGTT CCTGCATTCT CATTTACGAA 
AATGGATCTG ATGAAGCGAC ATGCTTTACT CCGCCAATAA AACCAGCTCA TCCAGTCACT 
GAACAGATCA GTGGGGAAAG AGTGGTTTTA AAGGTGACAC CTGTGTGTCC TGCAACAGAA 
GAGCTCAGGT TACTGTACAA AACAAAAGAA GAGAAAGACT GGAAATCTCA GTCTGTGAAA 
AAGAGTCAAG ATACAGTTAT TCTGACTGAT CTCAGACCAG ACACTGAGTA TGAGATGAAA 
TTTGCAGCCG TGGGGAAGCT GAACTACACC ATAGACAGTG ATGTGATTCA CCTCAGAGTT 
ATTGACAAAA ACCTCACTGA TGCCACAGAG TCCATTTTAG AAAAGCTGAG CTTGATAGAA 
GACAAGTGCA GTAAACTGAT GGAGGGCAAG AGTGCAGTCA CATTTAGAGC AATTCGCAAC 
AAGTTTGAAG ACATGAAGAG ACACTGTCAG ACTTACAAAC AAGACAGAAT TAAATCCACA 
ATCCAGTCAG TTCAAGCCTG TGAAAAAGAC ATCAGTGCTC TGACAGACCT TCTACAAGCT 
CATCATGAGT CTCCATTTAA CATAAGTGAT ATTGTGGAGT GGATCAGAGT GAAAGAAACC 
GAATCAAAAG CAGTTGAGAA GATTCTTCAA CAGCTGCTGG AAACTGGAGC TGAAGTGAAC 
AATAGTTTAG ATGAATATTT GTTGGATGTT AGTTTTGAGA ATGTGGTGTG TTACACATTC 
ACTTCTCTTG ATCTGCCAGA CGACCTGCTT TCTGATCAAG AAGATTTCCT GAAACCTGAA 
ATGATAAGGA GAAATTCTGA TAAGAAACCC AATGCTGTGT CTGAAACTTG GCTCACAGGA 
AGTACCAGAG AGAAATTGAA AAAACAATTA GAAATGTTTA AAGAGCTCAA ATGTTCATAT 
GGCAGTCACT CAACAAAGTT TTTGGTTATA TCAGAAGATC AGACAAACCA TCCAGGTTCC 
TGTATTCTCC TTTATGAAAA TGGATCTTAT AAAGCGAAAT GCTTTACTCC GCCAATAAAA 
CCAGCTGAGC CCATCATTGA ACAGGTCATC GGGGAAAGAG TGGTTTTGAA GGTGACATCT 
GTGTGTCCTG CAACAGAAGA GCTCAGGTTA CTGTACAAAA CAAAAGAAGA GAACGACTGG 
AAATCTCAGT CTGTGAAAAA GAGTCAAGAT ACAGTTATTC TGACTGATCT CAGACCAGAC 
ACTGAGTATG AGATGAAATT TGCAGCTGTG GGGAAGCTGA ACTACACCAT AGACAGTGAT 
GTGATTCACC TTAGAGTTAT TGACAAGAAA CTCACTGATG CCACAGAGTC CATTTTAGAA 
AAGCTGAGCT TGATAGAAGA CAAGTGCAGT AAACTGATGG AGGACAAGAG TGCAGTCACT 
TTTAGAGCAA TTCACAACAA GTTTGAAGAC ATGAGAAGAC ACTGTCAGAT CTACAAACAA 
GACTTAGAAA TTAAAATTAC ATCCACAATG CAGTCAGTTC AAACCTGTGA AAAAGACATC 
AGTGCTCTGA CAGACCTTCT ACAAGCTCAT CATGAGTCTC CATTTAATGA AAGTGATCTT 
ATAGAGTGGA TCAGAGTGAA AGAAACCGAA TCAAATGCAG TTGAGAAGAT TCTTCAACAG 
CTGCTGGACT CTGGAACTGA GGTGAACAAT AGTTTAGATG AATATTTGTT TGATGTTAGT 
TTTGAGAATG TGGTGTGTTA CACATTCACT TCTCTTGATC TGCCAGACAA CCTGCTTTCT 
AAACAAGGAG ATTTCCTGAA ACCTGAAATA ATGAGGAGAA ATTCTAATAA GAACCCCAAT 
TCTGTGTCTG AAACTTGGCT CACAGGAAGT ATCAGAGAGA CTATGAAGAA GCATTTGGAA 
ATATTTAATG AGCTCAAATG TTCATATGGC AGTCACTCAT CAACATTTTT GGTTGTATAT 
AAAAGTCACA CAAACCATCC AGGTTCCTGC ATTCTTATTT ATGAAAATGG ATCTGATAAA 
GCGACATGCT TCACTCCTCC AATAAAACCA GCTAGTCCAG TCATTGAACA GATTGGTGAG 
GATATAGTGG TTTTGAAGGC AACACCATTA TGTCCTGCAA CACAAGAGCT CAGGTTACTG 
TACAAAAAAA AAGAAGAGAA TGATTGGAAA TCTCAGTCTG TGAAAACGAG TGGAGATGAA 
GTTTTTCTGA CTGGTCTCAG ACCTGACACT GAGTATAAGA TGAAATATGT GGCCTTGGGG 
AAGCTGAACT ACACCACAGA CAGTGATGTC ATCGGAGTTA CTACACGTGT GGTCAGTGAG 
AAACAGTGTG CCTTGCCTTA TTCATGCAAT ACTAATTTCA CATATTTTAA CAGTGAATTA 
TTGAACAGTA ACTCTATGCA GCTAAATGAG ACTGGCAAAT ATCTGCAGGA TAAATCTGAA 
CAAAAACTAT CGATGACAAA GGAGCCAAGG AACAACCAAC CAATGTCATT TGATTCTTCA 
GAGGAGGTCA AAGTGTGCAC TGTTTTGGCT GGAAAGACCA ATAACTGTCA AACACATTTT 
ATTGCATCAC TGCGAGCCCG AATTGAGCAT CTGACGGAAG TTCGTTCAGT AGACGAGAGT 
GACATTGTTT TAATTTTCTG TCCAATTGTT TCTCGAGCTG GAACTGACAT TGATGCAGCC 
ATTCACAGAT GCTATACAAA TGCCTCTAAG CTGACTGTTC TAGTGGTGCT TCACCACACG 
TTTGACCCAG AGAAAACTGT ATCAGACAGC AGCAAAATTG TCAACAGGGA GAATCTGCTC 
ACAGTGGACT GTCTGTTCTA TGAGGATGCA GGATTACTGC AGTGTCAGAG GAATTCAGAT 
GCATATGACA AATCTGTCAA CTGGTTAATA GCGCAGGGCA AAAAAACAGG TCTTAAAATC 
TACCCAAAGA AATCACCTGA TAGTCAATCC AATGGTCGGA AATTCTGGAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100148510 ( XM_001921861.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001921896.3 Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X4, mRNA.

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CloneID	ODa10352
Clone ID Related Accession (Same CDS sequence)	XM_021476448.1 , XM_001921861.6 , XM_001921861.7
Accession Version	XM_001921861.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein LOC100148510 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001921861.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAGTA TGGACTTGGG AGTGGGAGTT AACATTGTTG AAACAGCAGC TCTGGGGAGA 
CCCTTCAGGC TGGGGATGCT GTACGACTGC AGAAAAGATG CATTTGTACC AGGAATCACA 
CTGTGGGATG AAGAGCAACT GCAGCAGAGC ATAAAAATCC ATCCTCAAAT TAAAACAGAT 
TTCTGTGTTA CAAATTCAGA CTTTATTGAG GCAAAATGCC AATTACTGAA CATTAATGAT 
TGTCTAAAAC TGAGTTTTTT AAGTGGACTC ATCAATGTTA GTGGAGCAGC CAAGTATCTC 
AGTGACACCA AGAAATCCTT CAAACAGCAG AGACTGACCC TACATTATCA TTCAACCACC 
AAACGTGAAG AACTGACTGT GAACCACTTG CCATCTGAAA ATATAACCCA CTATGAAGTG 
TTTGAGAATG ATGCAGCGAC ACATGTGGTG ACAGCTGTGC TGTATGGGGC CAATGCTTGC 
TTTGTTTTTG ACAGAGAAGC TTCATTAGTT GAAGACCAAA GCACTGTAGA CAGAGACGTA 
AAGGCTGCCT TAGAAAAACT AAAAGGGATT TCAGAAAGTA CACAGATTGA TCTGAGCTTG 
GATGACAATC AAAAGACTGT TTTTGAGAAA TTCAGCTGCA CATTTTATGG TGATTTCCAG 
TTAAAATCTA ATCCAGCCTC TTTTGAAGAA GCTCTGAGGG TTTTTGCTGA TCTTCCTAAA 
CTGCTGGGAG AAACCAAGGA GTGTGCAGTT CCACTGAGAG TGTGGCTTTA TCCACTGGAC 
AAACTGCATT CACATTATGT GAAACTTCAA CAAGAAATCA GCATAGATCT AATCAGAAAT 
GTTGAATCAG TATTTGAGAG CTTAAGGACC ACTGAAATGA AATGCAGTGA TCTTCTGAAT 
GATACACCAT CTTTGGCTTT TGCAGGATTT CATGATAAAA TAATGATTAT GAAGCAAAAC 
TGCTGCAACT ATAAGCTGAG CTTCATGAAG AAACTTGGAT CGCTATTGCC AAAAATCCGT 
AGTGATACAG AAAATGAGAC AGCCCTGATT GAGCTCCTCC ATGATCATGA AAAATGTCCA 
TTCAGAAAAA GGGATCTTGA GAAATGGGTG AAAGAAAAAG AGAATGAATC CGTCATTATT 
AAAACTCTTC TCAGACAATT GACTGATTCT GGAGCAACAG TGGAAAATAA TCTAGATGGA 
ATTTTAATGG ATCTTAATGT TGAAAATGTA GTAAGCTACA CTTTCACATC ACTTGAGTGT 
CCAGATGTGC TCCTTACTAA ACAAAAGGCT TTCCTGAATC CATCAACAAT AGAAAACAAC 
AGTGAGGATG CTTCTGTTTT AAGTAAAAAA TCTGGGTTCA CATCTGACAT CAAAACCACA 
ATGAGGAGCA ACTTGATCAT CTTCAAAAAC TTGATGGAAT CAAAAATATG TAAACCAGCC 
AAATATATTG TTGCATCGAA GGAAATACCA AATCCAGGTT CCTGCATTCT CATTTACGAA 
AATGGATCTG ATGAAGCGAC ATGCTTTACT CCGCCAATAA AACCAGCTCA TCCAGTCACT 
GAACAGATCA GTGGGGAAAG AGTGGTTTTA AAGGTGACAC CTGTGTGTCC TGCAACAGAA 
GAGCTCAGGT TACTGTACAA AACAAAAGAA GAGAAAGACT GGAAATCTCA GTCTGTGAAA 
AAGAGTCAAG ATACAGTTAT TCTGACTGAT CTCAGACCAG ACACTGAGTA TGAGATGAAA 
TTTGCAGCCG TGGGGAAGCT GAACTACACC ATAGACAGTG ATGTGATTCA CCTCAGAGTT 
ATTGACAAAA ACCTCACTGA TGCCACAGAG TCCATTTTAG AAAAGCTGAG CTTGATAGAA 
GACAAGTGCA GTAAACTGAT GGAGGGCAAG AGTGCAGTCA CATTTAGAGC AATTCGCAAC 
AAGTTTGAAG ACATGAAGAG ACACTGTCAG ACTTACAAAC AAGACAGAAT TAAATCCACA 
ATCCAGTCAG TTCAAGCCTG TGAAAAAGAC ATCAGTGCTC TGACAGACCT TCTACAAGCT 
CATCATGAGT CTCCATTTAA CATAAGTGAT ATTGTGGAGT GGATCAGAGT GAAAGAAACC 
GAATCAAAAG CAGTTGAGAA GATTCTTCAA CAGCTGCTGG AAACTGGAGC TGAAGTGAAC 
AATAGTTTAG ATGAATATTT GTTGGATGTT AGTTTTGAGA ATGTGGTGTG TTACACATTC 
ACTTCTCTTG ATCTGCCAGA CGACCTGCTT TCTGATCAAG AAGATTTCCT GAAACCTGAA 
ATGATAAGGA GAAATTCTGA TAAGAAACCC AATGCTGTGT CTGAAACTTG GCTCACAGGA 
AGTACCAGAG AGAAATTGAA AAAACAATTA GAAATGTTTA AAGAGCTCAA ATGTTCATAT 
GGCAGTCACT CAACAAAGTT TTTGGTTATA TCAGAAGATC AGACAAACCA TCCAGGTTCC 
TGTATTCTCC TTTATGAAAA TGGATCTTAT AAAGCGAAAT GCTTTACTCC GCCAATAAAA 
CCAGCTGAGC CCATCATTGA ACAGGTCATC GGGGAAAGAG TGGTTTTGAA GGTGACATCT 
GTGTGTCCTG CAACAGAAGA GCTCAGGTTA CTGTACAAAA CAAAAGAAGA GAACGACTGG 
AAATCTCAGT CTGTGAAAAA GAGTCAAGAT ACAGTTATTC TGACTGATCT CAGACCAGAC 
ACTGAGTATG AGATGAAATT TGCAGCTGTG GGGAAGCTGA ACTACACCAT AGACAGTGAT 
GTGATTCACC TTAGAGTTAT TGACAAGAAA CTCACTGATG CCACAGAGTC CATTTTAGAA 
AAGCTGAGCT TGATAGAAGA CAAGTGCAGT AAACTGATGG AGGACAAGAG TGCAGTCACT 
TTTAGAGCAA TTCACAACAA GTTTGAAGAC ATGAGAAGAC ACTGTCAGAT CTACAAACAA 
GACTTAGAAA TTAAAATTAC ATCCACAATG CAGTCAGTTC AAACCTGTGA AAAAGACATC 
AGTGCTCTGA CAGACCTTCT ACAAGCTCAT CATGAGTCTC CATTTAATGA AAGTGATCTT 
ATAGAGTGGA TCAGAGTGAA AGAAACCGAA TCAAATGCAG TTGAGAAGAT TCTTCAACAG 
CTGCTGGACT CTGGAACTGA GGTGAACAAT AGTTTAGATG AATATTTGTT TGATGTTAGT 
TTTGAGAATG TGGTGTGTTA CACATTCACT TCTCTTGATC TGCCAGACAA CCTGCTTTCT 
AAACAAGGAG ATTTCCTGAA ACCTGAAATA ATGAGGAGAA ATTCTAATAA GAACCCCAAT 
TCTGTGTCTG AAACTTGGCT CACAGGAAGT ATCAGAGAGA CTATGAAGAA GCATTTGGAA 
ATATTTAATG AGCTCAAATG TTCATATGGC AGTCACTCAT CAACATTTTT GGTTGTATAT 
AAAAGTCACA CAAACCATCC AGGTTCCTGC ATTCTTATTT ATGAAAATGG ATCTGATAAA 
GCGACATGCT TCACTCCTCC AATAAAACCA GCTAGTCCAG TCATTGAACA GATTGGTGAG 
GATATAGTGG TTTTGAAGGC AACACCATTA TGTCCTGCAA CACAAGAGCT CAGGTTACTG 
TACAAAAAAA AAGAAGAGAA TGATTGGAAA TCTCAGTCTG TGAAAACGAG TGGAGATGAA 
GTTTTTCTGA CTGGTCTCAG ACCTGACACT GAGTATAAGA TGAAATATGT GGCCTTGGGG 
AAGCTGAACT ACACCACAGA CAGTGATGTC ATCGGAGTTA CTACACGTGT GGTCAGTGAG 
AAACAGTGTG CCTTGCCTTA TTCATGCAAT ACTAATTTCA CATATTTTAA CAGTGAATTA 
TTGAACAGTA ACTCTATGCA GCTAAATGAG ACTGGCAAAT ATCTGCAGGA TAAATCTGAA 
CAAAAACTAT CGATGACAAA GGAGCCAAGG AACAACCAAC CAATGTCATT TGATTCTTCA 
GAGGAGGTCA AAGTGTGCAC TGTTTTGGCT GGAAAGACCA ATAACTGTCA AACACATTTT 
ATTGCATCAC TGCGAGCCCG AATTGAGCAT CTGACGGAAG TTCGTTCAGT AGACGAGAGT 
GACATTGTTT TAATTTTCTG TCCAATTGTT TCTCGAGCTG GAACTGACAT TGATGCAGCC 
ATTCACAGAT GCTATACAAA TGCCTCTAAG CTGACTGTTC TAGTGGTGCT TCACCACACG 
TTTGACCCAG AGAAAACTGT ATCAGACAGC AGCAAAATTG TCAACAGGGA GAATCTGCTC 
ACAGTGGACT GTCTGTTCTA TGAGGATGCA GGATTACTGC AGTGTCAGAG GAATTCAGAT 
GCATATGACA AATCTGTCAA CTGGTTAATA GCGCAGGGCA AAAAAACAGG TCTTAAAATC 
TACCCAAAGA AATCACCTGA TAGTCAATCC AATGGTCGGA AATTCTGGAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001921896.3
CDS	301..4734
Translation	MRSMDLGVGVNIVETAALGRPFRLGMLYDCRKDAFVPGITLWDEEQLQQSIKIHPQIKTDFCVTNSDFIEAKCQLLNINDCLKLSFLSGLINVSGAAKYLSDTKKSFKQQRLTLHYHSTTKREELTVNHLPSENITHYEVFENDAATHVVTAVLYGANACFVFDREASLVEDQSTVDRDVKAALEKLKGISESTQIDLSLDDNQKTVFEKFSCTFYGDFQLKSNPASFEEALRVFADLPKLLGETKECAVPLRVWLYPLDKLHSHYVKLQQEISIDLIRNVESVFESLRTTEMKCSDLLNDTPSLAFAGFHDKIMIMKQNCCNYKLSFMKKLGSLLPKIRSDTENETALIELLHDHEKCPFRKRDLEKWVKEKENESVIIKTLLRQLTDSGATVENNLDGILMDLNVENVVSYTFTSLECPDVLLTKQKAFLNPSTIENNSEDASVLSKKSGFTSDIKTTMRSNLIIFKNLMESKICKPAKYIVASKEIPNPGSCILIYENGSDEATCFTPPIKPAHPVTEQISGERVVLKVTPVCPATEELRLLYKTKEEKDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKNLTDATESILEKLSLIEDKCSKLMEGKSAVTFRAIRNKFEDMKRHCQTYKQDRIKSTIQSVQACEKDISALTDLLQAHHESPFNISDIVEWIRVKETESKAVEKILQQLLETGAEVNNSLDEYLLDVSFENVVCYTFTSLDLPDDLLSDQEDFLKPEMIRRNSDKKPNAVSETWLTGSTREKLKKQLEMFKELKCSYGSHSTKFLVISEDQTNHPGSCILLYENGSYKAKCFTPPIKPAEPIIEQVIGERVVLKVTSVCPATEELRLLYKTKEENDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKKLTDATESILEKLSLIEDKCSKLMEDKSAVTFRAIHNKFEDMRRHCQIYKQDLEIKITSTMQSVQTCEKDISALTDLLQAHHESPFNESDLIEWIRVKETESNAVEKILQQLLDSGTEVNNSLDEYLFDVSFENVVCYTFTSLDLPDNLLSKQGDFLKPEIMRRNSNKNPNSVSETWLTGSIRETMKKHLEIFNELKCSYGSHSSTFLVVYKSHTNHPGSCILIYENGSDKATCFTPPIKPASPVIEQIGEDIVVLKATPLCPATQELRLLYKKKEENDWKSQSVKTSGDEVFLTGLRPDTEYKMKYVALGKLNYTTDSDVIGVTTRVVSEKQCALPYSCNTNFTYFNSELLNSNSMQLNETGKYLQDKSEQKLSMTKEPRNNQPMSFDSSEEVKVCTVLAGKTNNCQTHFIASLRARIEHLTEVRSVDESDIVLIFCPIVSRAGTDIDAAIHRCYTNASKLTVLVVLHHTFDPEKTVSDSSKIVNRENLLTVDCLFYEDAGLLQCQRNSDAYDKSVNWLIAQGKKTGLKIYPKKSPDSQSNGRKFWR

Target ORF information:

RefSeq Version	XM_001921861.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001921861.6

ORF Insert Sequence:

ATGCGCAGTA TGGACTTGGG AGTGGGAGTT AACATTGTTG AAACAGCAGC TCTGGGGAGA 
CCCTTCAGGC TGGGGATGCT GTACGACTGC AGAAAAGATG CATTTGTACC AGGAATCACA 
CTGTGGGATG AAGAGCAACT GCAGCAGAGC ATAAAAATCC ATCCTCAAAT TAAAACAGAT 
TTCTGTGTTA CAAATTCAGA CTTTATTGAG GCAAAATGCC AATTACTGAA CATTAATGAT 
TGTCTAAAAC TGAGTTTTTT AAGTGGACTC ATCAATGTTA GTGGAGCAGC CAAGTATCTC 
AGTGACACCA AGAAATCCTT CAAACAGCAG AGACTGACCC TACATTATCA TTCAACCACC 
AAACGTGAAG AACTGACTGT GAACCACTTG CCATCTGAAA ATATAACCCA CTATGAAGTG 
TTTGAGAATG ATGCAGCGAC ACATGTGGTG ACAGCTGTGC TGTATGGGGC CAATGCTTGC 
TTTGTTTTTG ACAGAGAAGC TTCATTAGTT GAAGACCAAA GCACTGTAGA CAGAGACGTA 
AAGGCTGCCT TAGAAAAACT AAAAGGGATT TCAGAAAGTA CACAGATTGA TCTGAGCTTG 
GATGACAATC AAAAGACTGT TTTTGAGAAA TTCAGCTGCA CATTTTATGG TGATTTCCAG 
TTAAAATCTA ATCCAGCCTC TTTTGAAGAA GCTCTGAGGG TTTTTGCTGA TCTTCCTAAA 
CTGCTGGGAG AAACCAAGGA GTGTGCAGTT CCACTGAGAG TGTGGCTTTA TCCACTGGAC 
AAACTGCATT CACATTATGT GAAACTTCAA CAAGAAATCA GCATAGATCT AATCAGAAAT 
GTTGAATCAG TATTTGAGAG CTTAAGGACC ACTGAAATGA AATGCAGTGA TCTTCTGAAT 
GATACACCAT CTTTGGCTTT TGCAGGATTT CATGATAAAA TAATGATTAT GAAGCAAAAC 
TGCTGCAACT ATAAGCTGAG CTTCATGAAG AAACTTGGAT CGCTATTGCC AAAAATCCGT 
AGTGATACAG AAAATGAGAC AGCCCTGATT GAGCTCCTCC ATGATCATGA AAAATGTCCA 
TTCAGAAAAA GGGATCTTGA GAAATGGGTG AAAGAAAAAG AGAATGAATC CGTCATTATT 
AAAACTCTTC TCAGACAATT GACTGATTCT GGAGCAACAG TGGAAAATAA TCTAGATGGA 
ATTTTAATGG ATCTTAATGT TGAAAATGTA GTAAGCTACA CTTTCACATC ACTTGAGTGT 
CCAGATGTGC TCCTTACTAA ACAAAAGGCT TTCCTGAATC CATCAACAAT AGAAAACAAC 
AGTGAGGATG CTTCTGTTTT AAGTAAAAAA TCTGGGTTCA CATCTGACAT CAAAACCACA 
ATGAGGAGCA ACTTGATCAT CTTCAAAAAC TTGATGGAAT CAAAAATATG TAAACCAGCC 
AAATATATTG TTGCATCGAA GGAAATACCA AATCCAGGTT CCTGCATTCT CATTTACGAA 
AATGGATCTG ATGAAGCGAC ATGCTTTACT CCGCCAATAA AACCAGCTCA TCCAGTCACT 
GAACAGATCA GTGGGGAAAG AGTGGTTTTA AAGGTGACAC CTGTGTGTCC TGCAACAGAA 
GAGCTCAGGT TACTGTACAA AACAAAAGAA GAGAAAGACT GGAAATCTCA GTCTGTGAAA 
AAGAGTCAAG ATACAGTTAT TCTGACTGAT CTCAGACCAG ACACTGAGTA TGAGATGAAA 
TTTGCAGCCG TGGGGAAGCT GAACTACACC ATAGACAGTG ATGTGATTCA CCTCAGAGTT 
ATTGACAAAA ACCTCACTGA TGCCACAGAG TCCATTTTAG AAAAGCTGAG CTTGATAGAA 
GACAAGTGCA GTAAACTGAT GGAGGGCAAG AGTGCAGTCA CATTTAGAGC AATTCGCAAC 
AAGTTTGAAG ACATGAAGAG ACACTGTCAG ACTTACAAAC AAGACAGAAT TAAATCCACA 
ATCCAGTCAG TTCAAGCCTG TGAAAAAGAC ATCAGTGCTC TGACAGACCT TCTACAAGCT 
CATCATGAGT CTCCATTTAA CATAAGTGAT ATTGTGGAGT GGATCAGAGT GAAAGAAACC 
GAATCAAAAG CAGTTGAGAA GATTCTTCAA CAGCTGCTGG AAACTGGAGC TGAAGTGAAC 
AATAGTTTAG ATGAATATTT GTTGGATGTT AGTTTTGAGA ATGTGGTGTG TTACACATTC 
ACTTCTCTTG ATCTGCCAGA CGACCTGCTT TCTGATCAAG AAGATTTCCT GAAACCTGAA 
ATGATAAGGA GAAATTCTGA TAAGAAACCC AATGCTGTGT CTGAAACTTG GCTCACAGGA 
AGTACCAGAG AGAAATTGAA AAAACAATTA GAAATGTTTA AAGAGCTCAA ATGTTCATAT 
GGCAGTCACT CAACAAAGTT TTTGGTTATA TCAGAAGATC AGACAAACCA TCCAGGTTCC 
TGTATTCTCC TTTATGAAAA TGGATCTTAT AAAGCGAAAT GCTTTACTCC GCCAATAAAA 
CCAGCTGAGC CCATCATTGA ACAGGTCATC GGGGAAAGAG TGGTTTTGAA GGTGACATCT 
GTGTGTCCTG CAACAGAAGA GCTCAGGTTA CTGTACAAAA CAAAAGAAGA GAACGACTGG 
AAATCTCAGT CTGTGAAAAA GAGTCAAGAT ACAGTTATTC TGACTGATCT CAGACCAGAC 
ACTGAGTATG AGATGAAATT TGCAGCTGTG GGGAAGCTGA ACTACACCAT AGACAGTGAT 
GTGATTCACC TTAGAGTTAT TGACAAGAAA CTCACTGATG CCACAGAGTC CATTTTAGAA 
AAGCTGAGCT TGATAGAAGA CAAGTGCAGT AAACTGATGG AGGACAAGAG TGCAGTCACT 
TTTAGAGCAA TTCACAACAA GTTTGAAGAC ATGAGAAGAC ACTGTCAGAT CTACAAACAA 
GACTTAGAAA TTAAAATTAC ATCCACAATG CAGTCAGTTC AAACCTGTGA AAAAGACATC 
AGTGCTCTGA CAGACCTTCT ACAAGCTCAT CATGAGTCTC CATTTAATGA AAGTGATCTT 
ATAGAGTGGA TCAGAGTGAA AGAAACCGAA TCAAATGCAG TTGAGAAGAT TCTTCAACAG 
CTGCTGGACT CTGGAACTGA GGTGAACAAT AGTTTAGATG AATATTTGTT TGATGTTAGT 
TTTGAGAATG TGGTGTGTTA CACATTCACT TCTCTTGATC TGCCAGACAA CCTGCTTTCT 
AAACAAGGAG ATTTCCTGAA ACCTGAAATA ATGAGGAGAA ATTCTAATAA GAACCCCAAT 
TCTGTGTCTG AAACTTGGCT CACAGGAAGT ATCAGAGAGA CTATGAAGAA GCATTTGGAA 
ATATTTAATG AGCTCAAATG TTCATATGGC AGTCACTCAT CAACATTTTT GGTTGTATAT 
AAAAGTCACA CAAACCATCC AGGTTCCTGC ATTCTTATTT ATGAAAATGG ATCTGATAAA 
GCGACATGCT TCACTCCTCC AATAAAACCA GCTAGTCCAG TCATTGAACA GATTGGTGAG 
GATATAGTGG TTTTGAAGGC AACACCATTA TGTCCTGCAA CACAAGAGCT CAGGTTACTG 
TACAAAAAAA AAGAAGAGAA TGATTGGAAA TCTCAGTCTG TGAAAACGAG TGGAGATGAA 
GTTTTTCTGA CTGGTCTCAG ACCTGACACT GAGTATAAGA TGAAATATGT GGCCTTGGGG 
AAGCTGAACT ACACCACAGA CAGTGATGTC ATCGGAGTTA CTACACGTGT GGTCAGTGAG 
AAACAGTGTG CCTTGCCTTA TTCATGCAAT ACTAATTTCA CATATTTTAA CAGTGAATTA 
TTGAACAGTA ACTCTATGCA GCTAAATGAG ACTGGCAAAT ATCTGCAGGA TAAATCTGAA 
CAAAAACTAT CGATGACAAA GGAGCCAAGG AACAACCAAC CAATGTCATT TGATTCTTCA 
GAGGAGGTCA AAGTGTGCAC TGTTTTGGCT GGAAAGACCA ATAACTGTCA AACACATTTT 
ATTGCATCAC TGCGAGCCCG AATTGAGCAT CTGACGGAAG TTCGTTCAGT AGACGAGAGT 
GACATTGTTT TAATTTTCTG TCCAATTGTT TCTCGAGCTG GAACTGACAT TGATGCAGCC 
ATTCACAGAT GCTATACAAA TGCCTCTAAG CTGACTGTTC TAGTGGTGCT TCACCACACG 
TTTGACCCAG AGAAAACTGT ATCAGACAGC AGCAAAATTG TCAACAGGGA GAATCTGCTC 
ACAGTGGACT GTCTGTTCTA TGAGGATGCA GGATTACTGC AGTGTCAGAG GAATTCAGAT 
GCATATGACA AATCTGTCAA CTGGTTAATA GCGCAGGGCA AAAAAACAGG TCTTAAAATC 
TACCCAAAGA AATCACCTGA TAGTCAATCC AATGGTCGGA AATTCTGGAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100148510 ( XM_001921861.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001921896.3 Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X1, mRNA.

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CloneID	ODa10352
Clone ID Related Accession (Same CDS sequence)	XM_021476448.1 , XM_001921861.6 , XM_001921861.7
Accession Version	XM_001921861.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC100148510 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001921861.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAGTA TGGACTTGGG AGTGGGAGTT AACATTGTTG AAACAGCAGC TCTGGGGAGA 
CCCTTCAGGC TGGGGATGCT GTACGACTGC AGAAAAGATG CATTTGTACC AGGAATCACA 
CTGTGGGATG AAGAGCAACT GCAGCAGAGC ATAAAAATCC ATCCTCAAAT TAAAACAGAT 
TTCTGTGTTA CAAATTCAGA CTTTATTGAG GCAAAATGCC AATTACTGAA CATTAATGAT 
TGTCTAAAAC TGAGTTTTTT AAGTGGACTC ATCAATGTTA GTGGAGCAGC CAAGTATCTC 
AGTGACACCA AGAAATCCTT CAAACAGCAG AGACTGACCC TACATTATCA TTCAACCACC 
AAACGTGAAG AACTGACTGT GAACCACTTG CCATCTGAAA ATATAACCCA CTATGAAGTG 
TTTGAGAATG ATGCAGCGAC ACATGTGGTG ACAGCTGTGC TGTATGGGGC CAATGCTTGC 
TTTGTTTTTG ACAGAGAAGC TTCATTAGTT GAAGACCAAA GCACTGTAGA CAGAGACGTA 
AAGGCTGCCT TAGAAAAACT AAAAGGGATT TCAGAAAGTA CACAGATTGA TCTGAGCTTG 
GATGACAATC AAAAGACTGT TTTTGAGAAA TTCAGCTGCA CATTTTATGG TGATTTCCAG 
TTAAAATCTA ATCCAGCCTC TTTTGAAGAA GCTCTGAGGG TTTTTGCTGA TCTTCCTAAA 
CTGCTGGGAG AAACCAAGGA GTGTGCAGTT CCACTGAGAG TGTGGCTTTA TCCACTGGAC 
AAACTGCATT CACATTATGT GAAACTTCAA CAAGAAATCA GCATAGATCT AATCAGAAAT 
GTTGAATCAG TATTTGAGAG CTTAAGGACC ACTGAAATGA AATGCAGTGA TCTTCTGAAT 
GATACACCAT CTTTGGCTTT TGCAGGATTT CATGATAAAA TAATGATTAT GAAGCAAAAC 
TGCTGCAACT ATAAGCTGAG CTTCATGAAG AAACTTGGAT CGCTATTGCC AAAAATCCGT 
AGTGATACAG AAAATGAGAC AGCCCTGATT GAGCTCCTCC ATGATCATGA AAAATGTCCA 
TTCAGAAAAA GGGATCTTGA GAAATGGGTG AAAGAAAAAG AGAATGAATC CGTCATTATT 
AAAACTCTTC TCAGACAATT GACTGATTCT GGAGCAACAG TGGAAAATAA TCTAGATGGA 
ATTTTAATGG ATCTTAATGT TGAAAATGTA GTAAGCTACA CTTTCACATC ACTTGAGTGT 
CCAGATGTGC TCCTTACTAA ACAAAAGGCT TTCCTGAATC CATCAACAAT AGAAAACAAC 
AGTGAGGATG CTTCTGTTTT AAGTAAAAAA TCTGGGTTCA CATCTGACAT CAAAACCACA 
ATGAGGAGCA ACTTGATCAT CTTCAAAAAC TTGATGGAAT CAAAAATATG TAAACCAGCC 
AAATATATTG TTGCATCGAA GGAAATACCA AATCCAGGTT CCTGCATTCT CATTTACGAA 
AATGGATCTG ATGAAGCGAC ATGCTTTACT CCGCCAATAA AACCAGCTCA TCCAGTCACT 
GAACAGATCA GTGGGGAAAG AGTGGTTTTA AAGGTGACAC CTGTGTGTCC TGCAACAGAA 
GAGCTCAGGT TACTGTACAA AACAAAAGAA GAGAAAGACT GGAAATCTCA GTCTGTGAAA 
AAGAGTCAAG ATACAGTTAT TCTGACTGAT CTCAGACCAG ACACTGAGTA TGAGATGAAA 
TTTGCAGCCG TGGGGAAGCT GAACTACACC ATAGACAGTG ATGTGATTCA CCTCAGAGTT 
ATTGACAAAA ACCTCACTGA TGCCACAGAG TCCATTTTAG AAAAGCTGAG CTTGATAGAA 
GACAAGTGCA GTAAACTGAT GGAGGGCAAG AGTGCAGTCA CATTTAGAGC AATTCGCAAC 
AAGTTTGAAG ACATGAAGAG ACACTGTCAG ACTTACAAAC AAGACAGAAT TAAATCCACA 
ATCCAGTCAG TTCAAGCCTG TGAAAAAGAC ATCAGTGCTC TGACAGACCT TCTACAAGCT 
CATCATGAGT CTCCATTTAA CATAAGTGAT ATTGTGGAGT GGATCAGAGT GAAAGAAACC 
GAATCAAAAG CAGTTGAGAA GATTCTTCAA CAGCTGCTGG AAACTGGAGC TGAAGTGAAC 
AATAGTTTAG ATGAATATTT GTTGGATGTT AGTTTTGAGA ATGTGGTGTG TTACACATTC 
ACTTCTCTTG ATCTGCCAGA CGACCTGCTT TCTGATCAAG AAGATTTCCT GAAACCTGAA 
ATGATAAGGA GAAATTCTGA TAAGAAACCC AATGCTGTGT CTGAAACTTG GCTCACAGGA 
AGTACCAGAG AGAAATTGAA AAAACAATTA GAAATGTTTA AAGAGCTCAA ATGTTCATAT 
GGCAGTCACT CAACAAAGTT TTTGGTTATA TCAGAAGATC AGACAAACCA TCCAGGTTCC 
TGTATTCTCC TTTATGAAAA TGGATCTTAT AAAGCGAAAT GCTTTACTCC GCCAATAAAA 
CCAGCTGAGC CCATCATTGA ACAGGTCATC GGGGAAAGAG TGGTTTTGAA GGTGACATCT 
GTGTGTCCTG CAACAGAAGA GCTCAGGTTA CTGTACAAAA CAAAAGAAGA GAACGACTGG 
AAATCTCAGT CTGTGAAAAA GAGTCAAGAT ACAGTTATTC TGACTGATCT CAGACCAGAC 
ACTGAGTATG AGATGAAATT TGCAGCTGTG GGGAAGCTGA ACTACACCAT AGACAGTGAT 
GTGATTCACC TTAGAGTTAT TGACAAGAAA CTCACTGATG CCACAGAGTC CATTTTAGAA 
AAGCTGAGCT TGATAGAAGA CAAGTGCAGT AAACTGATGG AGGACAAGAG TGCAGTCACT 
TTTAGAGCAA TTCACAACAA GTTTGAAGAC ATGAGAAGAC ACTGTCAGAT CTACAAACAA 
GACTTAGAAA TTAAAATTAC ATCCACAATG CAGTCAGTTC AAACCTGTGA AAAAGACATC 
AGTGCTCTGA CAGACCTTCT ACAAGCTCAT CATGAGTCTC CATTTAATGA AAGTGATCTT 
ATAGAGTGGA TCAGAGTGAA AGAAACCGAA TCAAATGCAG TTGAGAAGAT TCTTCAACAG 
CTGCTGGACT CTGGAACTGA GGTGAACAAT AGTTTAGATG AATATTTGTT TGATGTTAGT 
TTTGAGAATG TGGTGTGTTA CACATTCACT TCTCTTGATC TGCCAGACAA CCTGCTTTCT 
AAACAAGGAG ATTTCCTGAA ACCTGAAATA ATGAGGAGAA ATTCTAATAA GAACCCCAAT 
TCTGTGTCTG AAACTTGGCT CACAGGAAGT ATCAGAGAGA CTATGAAGAA GCATTTGGAA 
ATATTTAATG AGCTCAAATG TTCATATGGC AGTCACTCAT CAACATTTTT GGTTGTATAT 
AAAAGTCACA CAAACCATCC AGGTTCCTGC ATTCTTATTT ATGAAAATGG ATCTGATAAA 
GCGACATGCT TCACTCCTCC AATAAAACCA GCTAGTCCAG TCATTGAACA GATTGGTGAG 
GATATAGTGG TTTTGAAGGC AACACCATTA TGTCCTGCAA CACAAGAGCT CAGGTTACTG 
TACAAAAAAA AAGAAGAGAA TGATTGGAAA TCTCAGTCTG TGAAAACGAG TGGAGATGAA 
GTTTTTCTGA CTGGTCTCAG ACCTGACACT GAGTATAAGA TGAAATATGT GGCCTTGGGG 
AAGCTGAACT ACACCACAGA CAGTGATGTC ATCGGAGTTA CTACACGTGT GGTCAGTGAG 
AAACAGTGTG CCTTGCCTTA TTCATGCAAT ACTAATTTCA CATATTTTAA CAGTGAATTA 
TTGAACAGTA ACTCTATGCA GCTAAATGAG ACTGGCAAAT ATCTGCAGGA TAAATCTGAA 
CAAAAACTAT CGATGACAAA GGAGCCAAGG AACAACCAAC CAATGTCATT TGATTCTTCA 
GAGGAGGTCA AAGTGTGCAC TGTTTTGGCT GGAAAGACCA ATAACTGTCA AACACATTTT 
ATTGCATCAC TGCGAGCCCG AATTGAGCAT CTGACGGAAG TTCGTTCAGT AGACGAGAGT 
GACATTGTTT TAATTTTCTG TCCAATTGTT TCTCGAGCTG GAACTGACAT TGATGCAGCC 
ATTCACAGAT GCTATACAAA TGCCTCTAAG CTGACTGTTC TAGTGGTGCT TCACCACACG 
TTTGACCCAG AGAAAACTGT ATCAGACAGC AGCAAAATTG TCAACAGGGA GAATCTGCTC 
ACAGTGGACT GTCTGTTCTA TGAGGATGCA GGATTACTGC AGTGTCAGAG GAATTCAGAT 
GCATATGACA AATCTGTCAA CTGGTTAATA GCGCAGGGCA AAAAAACAGG TCTTAAAATC 
TACCCAAAGA AATCACCTGA TAGTCAATCC AATGGTCGGA AATTCTGGAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001921896.3
CDS	301..4734
Translation	MRSMDLGVGVNIVETAALGRPFRLGMLYDCRKDAFVPGITLWDEEQLQQSIKIHPQIKTDFCVTNSDFIEAKCQLLNINDCLKLSFLSGLINVSGAAKYLSDTKKSFKQQRLTLHYHSTTKREELTVNHLPSENITHYEVFENDAATHVVTAVLYGANACFVFDREASLVEDQSTVDRDVKAALEKLKGISESTQIDLSLDDNQKTVFEKFSCTFYGDFQLKSNPASFEEALRVFADLPKLLGETKECAVPLRVWLYPLDKLHSHYVKLQQEISIDLIRNVESVFESLRTTEMKCSDLLNDTPSLAFAGFHDKIMIMKQNCCNYKLSFMKKLGSLLPKIRSDTENETALIELLHDHEKCPFRKRDLEKWVKEKENESVIIKTLLRQLTDSGATVENNLDGILMDLNVENVVSYTFTSLECPDVLLTKQKAFLNPSTIENNSEDASVLSKKSGFTSDIKTTMRSNLIIFKNLMESKICKPAKYIVASKEIPNPGSCILIYENGSDEATCFTPPIKPAHPVTEQISGERVVLKVTPVCPATEELRLLYKTKEEKDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKNLTDATESILEKLSLIEDKCSKLMEGKSAVTFRAIRNKFEDMKRHCQTYKQDRIKSTIQSVQACEKDISALTDLLQAHHESPFNISDIVEWIRVKETESKAVEKILQQLLETGAEVNNSLDEYLLDVSFENVVCYTFTSLDLPDDLLSDQEDFLKPEMIRRNSDKKPNAVSETWLTGSTREKLKKQLEMFKELKCSYGSHSTKFLVISEDQTNHPGSCILLYENGSYKAKCFTPPIKPAEPIIEQVIGERVVLKVTSVCPATEELRLLYKTKEENDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKKLTDATESILEKLSLIEDKCSKLMEDKSAVTFRAIHNKFEDMRRHCQIYKQDLEIKITSTMQSVQTCEKDISALTDLLQAHHESPFNESDLIEWIRVKETESNAVEKILQQLLDSGTEVNNSLDEYLFDVSFENVVCYTFTSLDLPDNLLSKQGDFLKPEIMRRNSNKNPNSVSETWLTGSIRETMKKHLEIFNELKCSYGSHSSTFLVVYKSHTNHPGSCILIYENGSDKATCFTPPIKPASPVIEQIGEDIVVLKATPLCPATQELRLLYKKKEENDWKSQSVKTSGDEVFLTGLRPDTEYKMKYVALGKLNYTTDSDVIGVTTRVVSEKQCALPYSCNTNFTYFNSELLNSNSMQLNETGKYLQDKSEQKLSMTKEPRNNQPMSFDSSEEVKVCTVLAGKTNNCQTHFIASLRARIEHLTEVRSVDESDIVLIFCPIVSRAGTDIDAAIHRCYTNASKLTVLVVLHHTFDPEKTVSDSSKIVNRENLLTVDCLFYEDAGLLQCQRNSDAYDKSVNWLIAQGKKTGLKIYPKKSPDSQSNGRKFWR

Target ORF information:

RefSeq Version	XM_001921861.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001921861.7

ORF Insert Sequence:

ATGCGCAGTA TGGACTTGGG AGTGGGAGTT AACATTGTTG AAACAGCAGC TCTGGGGAGA 
CCCTTCAGGC TGGGGATGCT GTACGACTGC AGAAAAGATG CATTTGTACC AGGAATCACA 
CTGTGGGATG AAGAGCAACT GCAGCAGAGC ATAAAAATCC ATCCTCAAAT TAAAACAGAT 
TTCTGTGTTA CAAATTCAGA CTTTATTGAG GCAAAATGCC AATTACTGAA CATTAATGAT 
TGTCTAAAAC TGAGTTTTTT AAGTGGACTC ATCAATGTTA GTGGAGCAGC CAAGTATCTC 
AGTGACACCA AGAAATCCTT CAAACAGCAG AGACTGACCC TACATTATCA TTCAACCACC 
AAACGTGAAG AACTGACTGT GAACCACTTG CCATCTGAAA ATATAACCCA CTATGAAGTG 
TTTGAGAATG ATGCAGCGAC ACATGTGGTG ACAGCTGTGC TGTATGGGGC CAATGCTTGC 
TTTGTTTTTG ACAGAGAAGC TTCATTAGTT GAAGACCAAA GCACTGTAGA CAGAGACGTA 
AAGGCTGCCT TAGAAAAACT AAAAGGGATT TCAGAAAGTA CACAGATTGA TCTGAGCTTG 
GATGACAATC AAAAGACTGT TTTTGAGAAA TTCAGCTGCA CATTTTATGG TGATTTCCAG 
TTAAAATCTA ATCCAGCCTC TTTTGAAGAA GCTCTGAGGG TTTTTGCTGA TCTTCCTAAA 
CTGCTGGGAG AAACCAAGGA GTGTGCAGTT CCACTGAGAG TGTGGCTTTA TCCACTGGAC 
AAACTGCATT CACATTATGT GAAACTTCAA CAAGAAATCA GCATAGATCT AATCAGAAAT 
GTTGAATCAG TATTTGAGAG CTTAAGGACC ACTGAAATGA AATGCAGTGA TCTTCTGAAT 
GATACACCAT CTTTGGCTTT TGCAGGATTT CATGATAAAA TAATGATTAT GAAGCAAAAC 
TGCTGCAACT ATAAGCTGAG CTTCATGAAG AAACTTGGAT CGCTATTGCC AAAAATCCGT 
AGTGATACAG AAAATGAGAC AGCCCTGATT GAGCTCCTCC ATGATCATGA AAAATGTCCA 
TTCAGAAAAA GGGATCTTGA GAAATGGGTG AAAGAAAAAG AGAATGAATC CGTCATTATT 
AAAACTCTTC TCAGACAATT GACTGATTCT GGAGCAACAG TGGAAAATAA TCTAGATGGA 
ATTTTAATGG ATCTTAATGT TGAAAATGTA GTAAGCTACA CTTTCACATC ACTTGAGTGT 
CCAGATGTGC TCCTTACTAA ACAAAAGGCT TTCCTGAATC CATCAACAAT AGAAAACAAC 
AGTGAGGATG CTTCTGTTTT AAGTAAAAAA TCTGGGTTCA CATCTGACAT CAAAACCACA 
ATGAGGAGCA ACTTGATCAT CTTCAAAAAC TTGATGGAAT CAAAAATATG TAAACCAGCC 
AAATATATTG TTGCATCGAA GGAAATACCA AATCCAGGTT CCTGCATTCT CATTTACGAA 
AATGGATCTG ATGAAGCGAC ATGCTTTACT CCGCCAATAA AACCAGCTCA TCCAGTCACT 
GAACAGATCA GTGGGGAAAG AGTGGTTTTA AAGGTGACAC CTGTGTGTCC TGCAACAGAA 
GAGCTCAGGT TACTGTACAA AACAAAAGAA GAGAAAGACT GGAAATCTCA GTCTGTGAAA 
AAGAGTCAAG ATACAGTTAT TCTGACTGAT CTCAGACCAG ACACTGAGTA TGAGATGAAA 
TTTGCAGCCG TGGGGAAGCT GAACTACACC ATAGACAGTG ATGTGATTCA CCTCAGAGTT 
ATTGACAAAA ACCTCACTGA TGCCACAGAG TCCATTTTAG AAAAGCTGAG CTTGATAGAA 
GACAAGTGCA GTAAACTGAT GGAGGGCAAG AGTGCAGTCA CATTTAGAGC AATTCGCAAC 
AAGTTTGAAG ACATGAAGAG ACACTGTCAG ACTTACAAAC AAGACAGAAT TAAATCCACA 
ATCCAGTCAG TTCAAGCCTG TGAAAAAGAC ATCAGTGCTC TGACAGACCT TCTACAAGCT 
CATCATGAGT CTCCATTTAA CATAAGTGAT ATTGTGGAGT GGATCAGAGT GAAAGAAACC 
GAATCAAAAG CAGTTGAGAA GATTCTTCAA CAGCTGCTGG AAACTGGAGC TGAAGTGAAC 
AATAGTTTAG ATGAATATTT GTTGGATGTT AGTTTTGAGA ATGTGGTGTG TTACACATTC 
ACTTCTCTTG ATCTGCCAGA CGACCTGCTT TCTGATCAAG AAGATTTCCT GAAACCTGAA 
ATGATAAGGA GAAATTCTGA TAAGAAACCC AATGCTGTGT CTGAAACTTG GCTCACAGGA 
AGTACCAGAG AGAAATTGAA AAAACAATTA GAAATGTTTA AAGAGCTCAA ATGTTCATAT 
GGCAGTCACT CAACAAAGTT TTTGGTTATA TCAGAAGATC AGACAAACCA TCCAGGTTCC 
TGTATTCTCC TTTATGAAAA TGGATCTTAT AAAGCGAAAT GCTTTACTCC GCCAATAAAA 
CCAGCTGAGC CCATCATTGA ACAGGTCATC GGGGAAAGAG TGGTTTTGAA GGTGACATCT 
GTGTGTCCTG CAACAGAAGA GCTCAGGTTA CTGTACAAAA CAAAAGAAGA GAACGACTGG 
AAATCTCAGT CTGTGAAAAA GAGTCAAGAT ACAGTTATTC TGACTGATCT CAGACCAGAC 
ACTGAGTATG AGATGAAATT TGCAGCTGTG GGGAAGCTGA ACTACACCAT AGACAGTGAT 
GTGATTCACC TTAGAGTTAT TGACAAGAAA CTCACTGATG CCACAGAGTC CATTTTAGAA 
AAGCTGAGCT TGATAGAAGA CAAGTGCAGT AAACTGATGG AGGACAAGAG TGCAGTCACT 
TTTAGAGCAA TTCACAACAA GTTTGAAGAC ATGAGAAGAC ACTGTCAGAT CTACAAACAA 
GACTTAGAAA TTAAAATTAC ATCCACAATG CAGTCAGTTC AAACCTGTGA AAAAGACATC 
AGTGCTCTGA CAGACCTTCT ACAAGCTCAT CATGAGTCTC CATTTAATGA AAGTGATCTT 
ATAGAGTGGA TCAGAGTGAA AGAAACCGAA TCAAATGCAG TTGAGAAGAT TCTTCAACAG 
CTGCTGGACT CTGGAACTGA GGTGAACAAT AGTTTAGATG AATATTTGTT TGATGTTAGT 
TTTGAGAATG TGGTGTGTTA CACATTCACT TCTCTTGATC TGCCAGACAA CCTGCTTTCT 
AAACAAGGAG ATTTCCTGAA ACCTGAAATA ATGAGGAGAA ATTCTAATAA GAACCCCAAT 
TCTGTGTCTG AAACTTGGCT CACAGGAAGT ATCAGAGAGA CTATGAAGAA GCATTTGGAA 
ATATTTAATG AGCTCAAATG TTCATATGGC AGTCACTCAT CAACATTTTT GGTTGTATAT 
AAAAGTCACA CAAACCATCC AGGTTCCTGC ATTCTTATTT ATGAAAATGG ATCTGATAAA 
GCGACATGCT TCACTCCTCC AATAAAACCA GCTAGTCCAG TCATTGAACA GATTGGTGAG 
GATATAGTGG TTTTGAAGGC AACACCATTA TGTCCTGCAA CACAAGAGCT CAGGTTACTG 
TACAAAAAAA AAGAAGAGAA TGATTGGAAA TCTCAGTCTG TGAAAACGAG TGGAGATGAA 
GTTTTTCTGA CTGGTCTCAG ACCTGACACT GAGTATAAGA TGAAATATGT GGCCTTGGGG 
AAGCTGAACT ACACCACAGA CAGTGATGTC ATCGGAGTTA CTACACGTGT GGTCAGTGAG 
AAACAGTGTG CCTTGCCTTA TTCATGCAAT ACTAATTTCA CATATTTTAA CAGTGAATTA 
TTGAACAGTA ACTCTATGCA GCTAAATGAG ACTGGCAAAT ATCTGCAGGA TAAATCTGAA 
CAAAAACTAT CGATGACAAA GGAGCCAAGG AACAACCAAC CAATGTCATT TGATTCTTCA 
GAGGAGGTCA AAGTGTGCAC TGTTTTGGCT GGAAAGACCA ATAACTGTCA AACACATTTT 
ATTGCATCAC TGCGAGCCCG AATTGAGCAT CTGACGGAAG TTCGTTCAGT AGACGAGAGT 
GACATTGTTT TAATTTTCTG TCCAATTGTT TCTCGAGCTG GAACTGACAT TGATGCAGCC 
ATTCACAGAT GCTATACAAA TGCCTCTAAG CTGACTGTTC TAGTGGTGCT TCACCACACG 
TTTGACCCAG AGAAAACTGT ATCAGACAGC AGCAAAATTG TCAACAGGGA GAATCTGCTC 
ACAGTGGACT GTCTGTTCTA TGAGGATGCA GGATTACTGC AGTGTCAGAG GAATTCAGAT 
GCATATGACA AATCTGTCAA CTGGTTAATA GCGCAGGGCA AAAAAACAGG TCTTAAAATC 
TACCCAAAGA AATCACCTGA TAGTCAATCC AATGGTCGGA AATTCTGGAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100148510 ( XM_021476449.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332124.1 Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa10353
Clone ID Related Accession (Same CDS sequence)	XM_021476449.1 , XM_021476450.1 , XM_009301400.2 , XM_009301401.2 , XM_009301400.3 , XM_009301401.3
Accession Version	XM_021476449.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC100148510 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTGG GAGTGGGAGT TAACATTGTT GAAACAGCAG CTCTGGGGAG ACCCTTCAGG 
CTGGGGATGC TGTACGACTG CAGAAAAGAT GCATTTGTAC CAGGAATCAC ACTGTGGGAT 
GAAGAGCAAC TGCAGCAGAG CATAAAAATC CATCCTCAAA TTAAAACAGA TTTCTGTGTT 
ACAAATTCAG ACTTTATTGA GGCAAAATGC CAATTACTGA ACATTAATGA TTGTCTAAAA 
CTGAGTTTTT TAAGTGGACT CATCAATGTT AGTGGAGCAG CCAAGTATCT CAGTGACACC 
AAGAAATCCT TCAAACAGCA GAGACTGACC CTACATTATC ATTCAACCAC CAAACGTGAA 
GAACTGACTG TGAACCACTT GCCATCTGAA AATATAACCC ACTATGAAGT GTTTGAGAAT 
GATGCAGCGA CACATGTGGT GACAGCTGTG CTGTATGGGG CCAATGCTTG CTTTGTTTTT 
GACAGAGAAG CTTCATTAGT TGAAGACCAA AGCACTGTAG ACAGAGACGT AAAGGCTGCC 
TTAGAAAAAC TAAAAGGGAT TTCAGAAAGT ACACAGATTG ATCTGAGCTT GGATGACAAT 
CAAAAGACTG TTTTTGAGAA ATTCAGCTGC ACATTTTATG GTGATTTCCA GTTAAAATCT 
AATCCAGCCT CTTTTGAAGA AGCTCTGAGG GTTTTTGCTG ATCTTCCTAA ACTGCTGGGA 
GAAACCAAGG AGTGTGCAGT TCCACTGAGA GTGTGGCTTT ATCCACTGGA CAAACTGCAT 
TCACATTATG TGAAACTTCA ACAAGAAATC AGCATAGATC TAATCAGAAA TGTTGAATCA 
GTATTTGAGA GCTTAAGGAC CACTGAAATG AAATGCAGTG ATCTTCTGAA TGATACACCA 
TCTTTGGCTT TTGCAGGATT TCATGATAAA ATAATGATTA TGAAGCAAAA CTGCTGCAAC 
TATAAGCTGA GCTTCATGAA GAAACTTGGA TCGCTATTGC CAAAAATCCG TAGTGATACA 
GAAAATGAGA CAGCCCTGAT TGAGCTCCTC CATGATCATG AAAAATGTCC ATTCAGAAAA 
AGGGATCTTG AGAAATGGGT GAAAGAAAAA GAGAATGAAT CCGTCATTAT TAAAACTCTT 
CTCAGACAAT TGACTGATTC TGGAGCAACA GTGGAAAATA ATCTAGATGG AATTTTAATG 
GATCTTAATG TTGAAAATGT AGTAAGCTAC ACTTTCACAT CACTTGAGTG TCCAGATGTG 
CTCCTTACTA AACAAAAGGC TTTCCTGAAT CCATCAACAA TAGAAAACAA CAGTGAGGAT 
GCTTCTGTTT TAAGTAAAAA ATCTGGGTTC ACATCTGACA TCAAAACCAC AATGAGGAGC 
AACTTGATCA TCTTCAAAAA CTTGATGGAA TCAAAAATAT GTAAACCAGC CAAATATATT 
GTTGCATCGA AGGAAATACC AAATCCAGGT TCCTGCATTC TCATTTACGA AAATGGATCT 
GATGAAGCGA CATGCTTTAC TCCGCCAATA AAACCAGCTC ATCCAGTCAC TGAACAGATC 
AGTGGGGAAA GAGTGGTTTT AAAGGTGACA CCTGTGTGTC CTGCAACAGA AGAGCTCAGG 
TTACTGTACA AAACAAAAGA AGAGAAAGAC TGGAAATCTC AGTCTGTGAA AAAGAGTCAA 
GATACAGTTA TTCTGACTGA TCTCAGACCA GACACTGAGT ATGAGATGAA ATTTGCAGCC 
GTGGGGAAGC TGAACTACAC CATAGACAGT GATGTGATTC ACCTCAGAGT TATTGACAAA 
AACCTCACTG ATGCCACAGA GTCCATTTTA GAAAAGCTGA GCTTGATAGA AGACAAGTGC 
AGTAAACTGA TGGAGGGCAA GAGTGCAGTC ACATTTAGAG CAATTCGCAA CAAGTTTGAA 
GACATGAAGA GACACTGTCA GACTTACAAA CAAGACAGAA TTAAATCCAC AATCCAGTCA 
GTTCAAGCCT GTGAAAAAGA CATCAGTGCT CTGACAGACC TTCTACAAGC TCATCATGAG 
TCTCCATTTA ACATAAGTGA TATTGTGGAG TGGATCAGAG TGAAAGAAAC CGAATCAAAA 
GCAGTTGAGA AGATTCTTCA ACAGCTGCTG GAAACTGGAG CTGAAGTGAA CAATAGTTTA 
GATGAATATT TGTTGGATGT TAGTTTTGAG AATGTGGTGT GTTACACATT CACTTCTCTT 
GATCTGCCAG ACGACCTGCT TTCTGATCAA GAAGATTTCC TGAAACCTGA AATGATAAGG 
AGAAATTCTG ATAAGAAACC CAATGCTGTG TCTGAAACTT GGCTCACAGG AAGTACCAGA 
GAGAAATTGA AAAAACAATT AGAAATGTTT AAAGAGCTCA AATGTTCATA TGGCAGTCAC 
TCAACAAAGT TTTTGGTTAT ATCAGAAGAT CAGACAAACC ATCCAGGTTC CTGTATTCTC 
CTTTATGAAA ATGGATCTTA TAAAGCGAAA TGCTTTACTC CGCCAATAAA ACCAGCTGAG 
CCCATCATTG AACAGGTCAT CGGGGAAAGA GTGGTTTTGA AGGTGACATC TGTGTGTCCT 
GCAACAGAAG AGCTCAGGTT ACTGTACAAA ACAAAAGAAG AGAACGACTG GAAATCTCAG 
TCTGTGAAAA AGAGTCAAGA TACAGTTATT CTGACTGATC TCAGACCAGA CACTGAGTAT 
GAGATGAAAT TTGCAGCTGT GGGGAAGCTG AACTACACCA TAGACAGTGA TGTGATTCAC 
CTTAGAGTTA TTGACAAGAA ACTCACTGAT GCCACAGAGT CCATTTTAGA AAAGCTGAGC 
TTGATAGAAG ACAAGTGCAG TAAACTGATG GAGGACAAGA GTGCAGTCAC TTTTAGAGCA 
ATTCACAACA AGTTTGAAGA CATGAGAAGA CACTGTCAGA TCTACAAACA AGACTTAGAA 
ATTAAAATTA CATCCACAAT GCAGTCAGTT CAAACCTGTG AAAAAGACAT CAGTGCTCTG 
ACAGACCTTC TACAAGCTCA TCATGAGTCT CCATTTAATG AAAGTGATCT TATAGAGTGG 
ATCAGAGTGA AAGAAACCGA ATCAAATGCA GTTGAGAAGA TTCTTCAACA GCTGCTGGAC 
TCTGGAACTG AGGTGAACAA TAGTTTAGAT GAATATTTGT TTGATGTTAG TTTTGAGAAT 
GTGGTGTGTT ACACATTCAC TTCTCTTGAT CTGCCAGACA ACCTGCTTTC TAAACAAGGA 
GATTTCCTGA AACCTGAAAT AATGAGGAGA AATTCTAATA AGAACCCCAA TTCTGTGTCT 
GAAACTTGGC TCACAGGAAG TATCAGAGAG ACTATGAAGA AGCATTTGGA AATATTTAAT 
GAGCTCAAAT GTTCATATGG CAGTCACTCA TCAACATTTT TGGTTGTATA TAAAAGTCAC 
ACAAACCATC CAGGTTCCTG CATTCTTATT TATGAAAATG GATCTGATAA AGCGACATGC 
TTCACTCCTC CAATAAAACC AGCTAGTCCA GTCATTGAAC AGATTGGTGA GGATATAGTG 
GTTTTGAAGG CAACACCATT ATGTCCTGCA ACACAAGAGC TCAGGTTACT GTACAAAAAA 
AAAGAAGAGA ATGATTGGAA ATCTCAGTCT GTGAAAACGA GTGGAGATGA AGTTTTTCTG 
ACTGGTCTCA GACCTGACAC TGAGTATAAG ATGAAATATG TGGCCTTGGG GAAGCTGAAC 
TACACCACAG ACAGTGATGT CATCGGAGTT ACTACACGTG TGGTCAGTGA GAAACAGTGT 
GCCTTGCCTT ATTCATGCAA TACTAATTTC ACATATTTTA ACAGTGAATT ATTGAACAGT 
AACTCTATGC AGCTAAATGA GACTGGCAAA TATCTGCAGG ATAAATCTGA ACAAAAACTA 
TCGATGACAA AGGAGCCAAG GAACAACCAA CCAATGTCAT TTGATTCTTC AGAGGAGGTC 
AAAGTGTGCA CTGTTTTGGC TGGAAAGACC AATAACTGTC AAACACATTT TATTGCATCA 
CTGCGAGCCC GAATTGAGCA TCTGACGGAA GTTCGTTCAG TAGACGAGAG TGACATTGTT 
TTAATTTTCT GTCCAATTGT TTCTCGAGCT GGAACTGACA TTGATGCAGC CATTCACAGA 
TGCTATACAA ATGCCTCTAA GCTGACTGTT CTAGTGGTGC TTCACCACAC GTTTGACCCA 
GAGAAAACTG TATCAGACAG CAGCAAAATT GTCAACAGGG AGAATCTGCT CACAGTGGAC 
TGTCTGTTCT ATGAGGATGC AGGATTACTG CAGTGTCAGA GGAATTCAGA TGCATATGAC 
AAATCTGTCA ACTGGTTAAT AGCGCAGGGC AAAAAAACAG GTCTTAAAAT CTACCCAAAG 
AAATCACCTG ATAGTCAATC CAATGGTCGG AAATTCTGGA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332124.1
CDS	299..4723
Translation	MDLGVGVNIVETAALGRPFRLGMLYDCRKDAFVPGITLWDEEQLQQSIKIHPQIKTDFCVTNSDFIEAKCQLLNINDCLKLSFLSGLINVSGAAKYLSDTKKSFKQQRLTLHYHSTTKREELTVNHLPSENITHYEVFENDAATHVVTAVLYGANACFVFDREASLVEDQSTVDRDVKAALEKLKGISESTQIDLSLDDNQKTVFEKFSCTFYGDFQLKSNPASFEEALRVFADLPKLLGETKECAVPLRVWLYPLDKLHSHYVKLQQEISIDLIRNVESVFESLRTTEMKCSDLLNDTPSLAFAGFHDKIMIMKQNCCNYKLSFMKKLGSLLPKIRSDTENETALIELLHDHEKCPFRKRDLEKWVKEKENESVIIKTLLRQLTDSGATVENNLDGILMDLNVENVVSYTFTSLECPDVLLTKQKAFLNPSTIENNSEDASVLSKKSGFTSDIKTTMRSNLIIFKNLMESKICKPAKYIVASKEIPNPGSCILIYENGSDEATCFTPPIKPAHPVTEQISGERVVLKVTPVCPATEELRLLYKTKEEKDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKNLTDATESILEKLSLIEDKCSKLMEGKSAVTFRAIRNKFEDMKRHCQTYKQDRIKSTIQSVQACEKDISALTDLLQAHHESPFNISDIVEWIRVKETESKAVEKILQQLLETGAEVNNSLDEYLLDVSFENVVCYTFTSLDLPDDLLSDQEDFLKPEMIRRNSDKKPNAVSETWLTGSTREKLKKQLEMFKELKCSYGSHSTKFLVISEDQTNHPGSCILLYENGSYKAKCFTPPIKPAEPIIEQVIGERVVLKVTSVCPATEELRLLYKTKEENDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKKLTDATESILEKLSLIEDKCSKLMEDKSAVTFRAIHNKFEDMRRHCQIYKQDLEIKITSTMQSVQTCEKDISALTDLLQAHHESPFNESDLIEWIRVKETESNAVEKILQQLLDSGTEVNNSLDEYLFDVSFENVVCYTFTSLDLPDNLLSKQGDFLKPEIMRRNSNKNPNSVSETWLTGSIRETMKKHLEIFNELKCSYGSHSSTFLVVYKSHTNHPGSCILIYENGSDKATCFTPPIKPASPVIEQIGEDIVVLKATPLCPATQELRLLYKKKEENDWKSQSVKTSGDEVFLTGLRPDTEYKMKYVALGKLNYTTDSDVIGVTTRVVSEKQCALPYSCNTNFTYFNSELLNSNSMQLNETGKYLQDKSEQKLSMTKEPRNNQPMSFDSSEEVKVCTVLAGKTNNCQTHFIASLRARIEHLTEVRSVDESDIVLIFCPIVSRAGTDIDAAIHRCYTNASKLTVLVVLHHTFDPEKTVSDSSKIVNRENLLTVDCLFYEDAGLLQCQRNSDAYDKSVNWLIAQGKKTGLKIYPKKSPDSQSNGRKFWR

Target ORF information:

RefSeq Version	XM_021476449.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476449.1

ORF Insert Sequence:

ATGGACTTGG GAGTGGGAGT TAACATTGTT GAAACAGCAG CTCTGGGGAG ACCCTTCAGG 
CTGGGGATGC TGTACGACTG CAGAAAAGAT GCATTTGTAC CAGGAATCAC ACTGTGGGAT 
GAAGAGCAAC TGCAGCAGAG CATAAAAATC CATCCTCAAA TTAAAACAGA TTTCTGTGTT 
ACAAATTCAG ACTTTATTGA GGCAAAATGC CAATTACTGA ACATTAATGA TTGTCTAAAA 
CTGAGTTTTT TAAGTGGACT CATCAATGTT AGTGGAGCAG CCAAGTATCT CAGTGACACC 
AAGAAATCCT TCAAACAGCA GAGACTGACC CTACATTATC ATTCAACCAC CAAACGTGAA 
GAACTGACTG TGAACCACTT GCCATCTGAA AATATAACCC ACTATGAAGT GTTTGAGAAT 
GATGCAGCGA CACATGTGGT GACAGCTGTG CTGTATGGGG CCAATGCTTG CTTTGTTTTT 
GACAGAGAAG CTTCATTAGT TGAAGACCAA AGCACTGTAG ACAGAGACGT AAAGGCTGCC 
TTAGAAAAAC TAAAAGGGAT TTCAGAAAGT ACACAGATTG ATCTGAGCTT GGATGACAAT 
CAAAAGACTG TTTTTGAGAA ATTCAGCTGC ACATTTTATG GTGATTTCCA GTTAAAATCT 
AATCCAGCCT CTTTTGAAGA AGCTCTGAGG GTTTTTGCTG ATCTTCCTAA ACTGCTGGGA 
GAAACCAAGG AGTGTGCAGT TCCACTGAGA GTGTGGCTTT ATCCACTGGA CAAACTGCAT 
TCACATTATG TGAAACTTCA ACAAGAAATC AGCATAGATC TAATCAGAAA TGTTGAATCA 
GTATTTGAGA GCTTAAGGAC CACTGAAATG AAATGCAGTG ATCTTCTGAA TGATACACCA 
TCTTTGGCTT TTGCAGGATT TCATGATAAA ATAATGATTA TGAAGCAAAA CTGCTGCAAC 
TATAAGCTGA GCTTCATGAA GAAACTTGGA TCGCTATTGC CAAAAATCCG TAGTGATACA 
GAAAATGAGA CAGCCCTGAT TGAGCTCCTC CATGATCATG AAAAATGTCC ATTCAGAAAA 
AGGGATCTTG AGAAATGGGT GAAAGAAAAA GAGAATGAAT CCGTCATTAT TAAAACTCTT 
CTCAGACAAT TGACTGATTC TGGAGCAACA GTGGAAAATA ATCTAGATGG AATTTTAATG 
GATCTTAATG TTGAAAATGT AGTAAGCTAC ACTTTCACAT CACTTGAGTG TCCAGATGTG 
CTCCTTACTA AACAAAAGGC TTTCCTGAAT CCATCAACAA TAGAAAACAA CAGTGAGGAT 
GCTTCTGTTT TAAGTAAAAA ATCTGGGTTC ACATCTGACA TCAAAACCAC AATGAGGAGC 
AACTTGATCA TCTTCAAAAA CTTGATGGAA TCAAAAATAT GTAAACCAGC CAAATATATT 
GTTGCATCGA AGGAAATACC AAATCCAGGT TCCTGCATTC TCATTTACGA AAATGGATCT 
GATGAAGCGA CATGCTTTAC TCCGCCAATA AAACCAGCTC ATCCAGTCAC TGAACAGATC 
AGTGGGGAAA GAGTGGTTTT AAAGGTGACA CCTGTGTGTC CTGCAACAGA AGAGCTCAGG 
TTACTGTACA AAACAAAAGA AGAGAAAGAC TGGAAATCTC AGTCTGTGAA AAAGAGTCAA 
GATACAGTTA TTCTGACTGA TCTCAGACCA GACACTGAGT ATGAGATGAA ATTTGCAGCC 
GTGGGGAAGC TGAACTACAC CATAGACAGT GATGTGATTC ACCTCAGAGT TATTGACAAA 
AACCTCACTG ATGCCACAGA GTCCATTTTA GAAAAGCTGA GCTTGATAGA AGACAAGTGC 
AGTAAACTGA TGGAGGGCAA GAGTGCAGTC ACATTTAGAG CAATTCGCAA CAAGTTTGAA 
GACATGAAGA GACACTGTCA GACTTACAAA CAAGACAGAA TTAAATCCAC AATCCAGTCA 
GTTCAAGCCT GTGAAAAAGA CATCAGTGCT CTGACAGACC TTCTACAAGC TCATCATGAG 
TCTCCATTTA ACATAAGTGA TATTGTGGAG TGGATCAGAG TGAAAGAAAC CGAATCAAAA 
GCAGTTGAGA AGATTCTTCA ACAGCTGCTG GAAACTGGAG CTGAAGTGAA CAATAGTTTA 
GATGAATATT TGTTGGATGT TAGTTTTGAG AATGTGGTGT GTTACACATT CACTTCTCTT 
GATCTGCCAG ACGACCTGCT TTCTGATCAA GAAGATTTCC TGAAACCTGA AATGATAAGG 
AGAAATTCTG ATAAGAAACC CAATGCTGTG TCTGAAACTT GGCTCACAGG AAGTACCAGA 
GAGAAATTGA AAAAACAATT AGAAATGTTT AAAGAGCTCA AATGTTCATA TGGCAGTCAC 
TCAACAAAGT TTTTGGTTAT ATCAGAAGAT CAGACAAACC ATCCAGGTTC CTGTATTCTC 
CTTTATGAAA ATGGATCTTA TAAAGCGAAA TGCTTTACTC CGCCAATAAA ACCAGCTGAG 
CCCATCATTG AACAGGTCAT CGGGGAAAGA GTGGTTTTGA AGGTGACATC TGTGTGTCCT 
GCAACAGAAG AGCTCAGGTT ACTGTACAAA ACAAAAGAAG AGAACGACTG GAAATCTCAG 
TCTGTGAAAA AGAGTCAAGA TACAGTTATT CTGACTGATC TCAGACCAGA CACTGAGTAT 
GAGATGAAAT TTGCAGCTGT GGGGAAGCTG AACTACACCA TAGACAGTGA TGTGATTCAC 
CTTAGAGTTA TTGACAAGAA ACTCACTGAT GCCACAGAGT CCATTTTAGA AAAGCTGAGC 
TTGATAGAAG ACAAGTGCAG TAAACTGATG GAGGACAAGA GTGCAGTCAC TTTTAGAGCA 
ATTCACAACA AGTTTGAAGA CATGAGAAGA CACTGTCAGA TCTACAAACA AGACTTAGAA 
ATTAAAATTA CATCCACAAT GCAGTCAGTT CAAACCTGTG AAAAAGACAT CAGTGCTCTG 
ACAGACCTTC TACAAGCTCA TCATGAGTCT CCATTTAATG AAAGTGATCT TATAGAGTGG 
ATCAGAGTGA AAGAAACCGA ATCAAATGCA GTTGAGAAGA TTCTTCAACA GCTGCTGGAC 
TCTGGAACTG AGGTGAACAA TAGTTTAGAT GAATATTTGT TTGATGTTAG TTTTGAGAAT 
GTGGTGTGTT ACACATTCAC TTCTCTTGAT CTGCCAGACA ACCTGCTTTC TAAACAAGGA 
GATTTCCTGA AACCTGAAAT AATGAGGAGA AATTCTAATA AGAACCCCAA TTCTGTGTCT 
GAAACTTGGC TCACAGGAAG TATCAGAGAG ACTATGAAGA AGCATTTGGA AATATTTAAT 
GAGCTCAAAT GTTCATATGG CAGTCACTCA TCAACATTTT TGGTTGTATA TAAAAGTCAC 
ACAAACCATC CAGGTTCCTG CATTCTTATT TATGAAAATG GATCTGATAA AGCGACATGC 
TTCACTCCTC CAATAAAACC AGCTAGTCCA GTCATTGAAC AGATTGGTGA GGATATAGTG 
GTTTTGAAGG CAACACCATT ATGTCCTGCA ACACAAGAGC TCAGGTTACT GTACAAAAAA 
AAAGAAGAGA ATGATTGGAA ATCTCAGTCT GTGAAAACGA GTGGAGATGA AGTTTTTCTG 
ACTGGTCTCA GACCTGACAC TGAGTATAAG ATGAAATATG TGGCCTTGGG GAAGCTGAAC 
TACACCACAG ACAGTGATGT CATCGGAGTT ACTACACGTG TGGTCAGTGA GAAACAGTGT 
GCCTTGCCTT ATTCATGCAA TACTAATTTC ACATATTTTA ACAGTGAATT ATTGAACAGT 
AACTCTATGC AGCTAAATGA GACTGGCAAA TATCTGCAGG ATAAATCTGA ACAAAAACTA 
TCGATGACAA AGGAGCCAAG GAACAACCAA CCAATGTCAT TTGATTCTTC AGAGGAGGTC 
AAAGTGTGCA CTGTTTTGGC TGGAAAGACC AATAACTGTC AAACACATTT TATTGCATCA 
CTGCGAGCCC GAATTGAGCA TCTGACGGAA GTTCGTTCAG TAGACGAGAG TGACATTGTT 
TTAATTTTCT GTCCAATTGT TTCTCGAGCT GGAACTGACA TTGATGCAGC CATTCACAGA 
TGCTATACAA ATGCCTCTAA GCTGACTGTT CTAGTGGTGC TTCACCACAC GTTTGACCCA 
GAGAAAACTG TATCAGACAG CAGCAAAATT GTCAACAGGG AGAATCTGCT CACAGTGGAC 
TGTCTGTTCT ATGAGGATGC AGGATTACTG CAGTGTCAGA GGAATTCAGA TGCATATGAC 
AAATCTGTCA ACTGGTTAAT AGCGCAGGGC AAAAAAACAG GTCTTAAAAT CTACCCAAAG 
AAATCACCTG ATAGTCAATC CAATGGTCGG AAATTCTGGA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100148510 ( XM_021476450.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332125.1 Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X3, mRNA.

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CloneID	ODa10353
Clone ID Related Accession (Same CDS sequence)	XM_021476449.1 , XM_021476450.1 , XM_009301400.2 , XM_009301401.2 , XM_009301400.3 , XM_009301401.3
Accession Version	XM_021476450.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC100148510 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTGG GAGTGGGAGT TAACATTGTT GAAACAGCAG CTCTGGGGAG ACCCTTCAGG 
CTGGGGATGC TGTACGACTG CAGAAAAGAT GCATTTGTAC CAGGAATCAC ACTGTGGGAT 
GAAGAGCAAC TGCAGCAGAG CATAAAAATC CATCCTCAAA TTAAAACAGA TTTCTGTGTT 
ACAAATTCAG ACTTTATTGA GGCAAAATGC CAATTACTGA ACATTAATGA TTGTCTAAAA 
CTGAGTTTTT TAAGTGGACT CATCAATGTT AGTGGAGCAG CCAAGTATCT CAGTGACACC 
AAGAAATCCT TCAAACAGCA GAGACTGACC CTACATTATC ATTCAACCAC CAAACGTGAA 
GAACTGACTG TGAACCACTT GCCATCTGAA AATATAACCC ACTATGAAGT GTTTGAGAAT 
GATGCAGCGA CACATGTGGT GACAGCTGTG CTGTATGGGG CCAATGCTTG CTTTGTTTTT 
GACAGAGAAG CTTCATTAGT TGAAGACCAA AGCACTGTAG ACAGAGACGT AAAGGCTGCC 
TTAGAAAAAC TAAAAGGGAT TTCAGAAAGT ACACAGATTG ATCTGAGCTT GGATGACAAT 
CAAAAGACTG TTTTTGAGAA ATTCAGCTGC ACATTTTATG GTGATTTCCA GTTAAAATCT 
AATCCAGCCT CTTTTGAAGA AGCTCTGAGG GTTTTTGCTG ATCTTCCTAA ACTGCTGGGA 
GAAACCAAGG AGTGTGCAGT TCCACTGAGA GTGTGGCTTT ATCCACTGGA CAAACTGCAT 
TCACATTATG TGAAACTTCA ACAAGAAATC AGCATAGATC TAATCAGAAA TGTTGAATCA 
GTATTTGAGA GCTTAAGGAC CACTGAAATG AAATGCAGTG ATCTTCTGAA TGATACACCA 
TCTTTGGCTT TTGCAGGATT TCATGATAAA ATAATGATTA TGAAGCAAAA CTGCTGCAAC 
TATAAGCTGA GCTTCATGAA GAAACTTGGA TCGCTATTGC CAAAAATCCG TAGTGATACA 
GAAAATGAGA CAGCCCTGAT TGAGCTCCTC CATGATCATG AAAAATGTCC ATTCAGAAAA 
AGGGATCTTG AGAAATGGGT GAAAGAAAAA GAGAATGAAT CCGTCATTAT TAAAACTCTT 
CTCAGACAAT TGACTGATTC TGGAGCAACA GTGGAAAATA ATCTAGATGG AATTTTAATG 
GATCTTAATG TTGAAAATGT AGTAAGCTAC ACTTTCACAT CACTTGAGTG TCCAGATGTG 
CTCCTTACTA AACAAAAGGC TTTCCTGAAT CCATCAACAA TAGAAAACAA CAGTGAGGAT 
GCTTCTGTTT TAAGTAAAAA ATCTGGGTTC ACATCTGACA TCAAAACCAC AATGAGGAGC 
AACTTGATCA TCTTCAAAAA CTTGATGGAA TCAAAAATAT GTAAACCAGC CAAATATATT 
GTTGCATCGA AGGAAATACC AAATCCAGGT TCCTGCATTC TCATTTACGA AAATGGATCT 
GATGAAGCGA CATGCTTTAC TCCGCCAATA AAACCAGCTC ATCCAGTCAC TGAACAGATC 
AGTGGGGAAA GAGTGGTTTT AAAGGTGACA CCTGTGTGTC CTGCAACAGA AGAGCTCAGG 
TTACTGTACA AAACAAAAGA AGAGAAAGAC TGGAAATCTC AGTCTGTGAA AAAGAGTCAA 
GATACAGTTA TTCTGACTGA TCTCAGACCA GACACTGAGT ATGAGATGAA ATTTGCAGCC 
GTGGGGAAGC TGAACTACAC CATAGACAGT GATGTGATTC ACCTCAGAGT TATTGACAAA 
AACCTCACTG ATGCCACAGA GTCCATTTTA GAAAAGCTGA GCTTGATAGA AGACAAGTGC 
AGTAAACTGA TGGAGGGCAA GAGTGCAGTC ACATTTAGAG CAATTCGCAA CAAGTTTGAA 
GACATGAAGA GACACTGTCA GACTTACAAA CAAGACAGAA TTAAATCCAC AATCCAGTCA 
GTTCAAGCCT GTGAAAAAGA CATCAGTGCT CTGACAGACC TTCTACAAGC TCATCATGAG 
TCTCCATTTA ACATAAGTGA TATTGTGGAG TGGATCAGAG TGAAAGAAAC CGAATCAAAA 
GCAGTTGAGA AGATTCTTCA ACAGCTGCTG GAAACTGGAG CTGAAGTGAA CAATAGTTTA 
GATGAATATT TGTTGGATGT TAGTTTTGAG AATGTGGTGT GTTACACATT CACTTCTCTT 
GATCTGCCAG ACGACCTGCT TTCTGATCAA GAAGATTTCC TGAAACCTGA AATGATAAGG 
AGAAATTCTG ATAAGAAACC CAATGCTGTG TCTGAAACTT GGCTCACAGG AAGTACCAGA 
GAGAAATTGA AAAAACAATT AGAAATGTTT AAAGAGCTCA AATGTTCATA TGGCAGTCAC 
TCAACAAAGT TTTTGGTTAT ATCAGAAGAT CAGACAAACC ATCCAGGTTC CTGTATTCTC 
CTTTATGAAA ATGGATCTTA TAAAGCGAAA TGCTTTACTC CGCCAATAAA ACCAGCTGAG 
CCCATCATTG AACAGGTCAT CGGGGAAAGA GTGGTTTTGA AGGTGACATC TGTGTGTCCT 
GCAACAGAAG AGCTCAGGTT ACTGTACAAA ACAAAAGAAG AGAACGACTG GAAATCTCAG 
TCTGTGAAAA AGAGTCAAGA TACAGTTATT CTGACTGATC TCAGACCAGA CACTGAGTAT 
GAGATGAAAT TTGCAGCTGT GGGGAAGCTG AACTACACCA TAGACAGTGA TGTGATTCAC 
CTTAGAGTTA TTGACAAGAA ACTCACTGAT GCCACAGAGT CCATTTTAGA AAAGCTGAGC 
TTGATAGAAG ACAAGTGCAG TAAACTGATG GAGGACAAGA GTGCAGTCAC TTTTAGAGCA 
ATTCACAACA AGTTTGAAGA CATGAGAAGA CACTGTCAGA TCTACAAACA AGACTTAGAA 
ATTAAAATTA CATCCACAAT GCAGTCAGTT CAAACCTGTG AAAAAGACAT CAGTGCTCTG 
ACAGACCTTC TACAAGCTCA TCATGAGTCT CCATTTAATG AAAGTGATCT TATAGAGTGG 
ATCAGAGTGA AAGAAACCGA ATCAAATGCA GTTGAGAAGA TTCTTCAACA GCTGCTGGAC 
TCTGGAACTG AGGTGAACAA TAGTTTAGAT GAATATTTGT TTGATGTTAG TTTTGAGAAT 
GTGGTGTGTT ACACATTCAC TTCTCTTGAT CTGCCAGACA ACCTGCTTTC TAAACAAGGA 
GATTTCCTGA AACCTGAAAT AATGAGGAGA AATTCTAATA AGAACCCCAA TTCTGTGTCT 
GAAACTTGGC TCACAGGAAG TATCAGAGAG ACTATGAAGA AGCATTTGGA AATATTTAAT 
GAGCTCAAAT GTTCATATGG CAGTCACTCA TCAACATTTT TGGTTGTATA TAAAAGTCAC 
ACAAACCATC CAGGTTCCTG CATTCTTATT TATGAAAATG GATCTGATAA AGCGACATGC 
TTCACTCCTC CAATAAAACC AGCTAGTCCA GTCATTGAAC AGATTGGTGA GGATATAGTG 
GTTTTGAAGG CAACACCATT ATGTCCTGCA ACACAAGAGC TCAGGTTACT GTACAAAAAA 
AAAGAAGAGA ATGATTGGAA ATCTCAGTCT GTGAAAACGA GTGGAGATGA AGTTTTTCTG 
ACTGGTCTCA GACCTGACAC TGAGTATAAG ATGAAATATG TGGCCTTGGG GAAGCTGAAC 
TACACCACAG ACAGTGATGT CATCGGAGTT ACTACACGTG TGGTCAGTGA GAAACAGTGT 
GCCTTGCCTT ATTCATGCAA TACTAATTTC ACATATTTTA ACAGTGAATT ATTGAACAGT 
AACTCTATGC AGCTAAATGA GACTGGCAAA TATCTGCAGG ATAAATCTGA ACAAAAACTA 
TCGATGACAA AGGAGCCAAG GAACAACCAA CCAATGTCAT TTGATTCTTC AGAGGAGGTC 
AAAGTGTGCA CTGTTTTGGC TGGAAAGACC AATAACTGTC AAACACATTT TATTGCATCA 
CTGCGAGCCC GAATTGAGCA TCTGACGGAA GTTCGTTCAG TAGACGAGAG TGACATTGTT 
TTAATTTTCT GTCCAATTGT TTCTCGAGCT GGAACTGACA TTGATGCAGC CATTCACAGA 
TGCTATACAA ATGCCTCTAA GCTGACTGTT CTAGTGGTGC TTCACCACAC GTTTGACCCA 
GAGAAAACTG TATCAGACAG CAGCAAAATT GTCAACAGGG AGAATCTGCT CACAGTGGAC 
TGTCTGTTCT ATGAGGATGC AGGATTACTG CAGTGTCAGA GGAATTCAGA TGCATATGAC 
AAATCTGTCA ACTGGTTAAT AGCGCAGGGC AAAAAAACAG GTCTTAAAAT CTACCCAAAG 
AAATCACCTG ATAGTCAATC CAATGGTCGG AAATTCTGGA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332125.1
CDS	621..5045
Translation	MDLGVGVNIVETAALGRPFRLGMLYDCRKDAFVPGITLWDEEQLQQSIKIHPQIKTDFCVTNSDFIEAKCQLLNINDCLKLSFLSGLINVSGAAKYLSDTKKSFKQQRLTLHYHSTTKREELTVNHLPSENITHYEVFENDAATHVVTAVLYGANACFVFDREASLVEDQSTVDRDVKAALEKLKGISESTQIDLSLDDNQKTVFEKFSCTFYGDFQLKSNPASFEEALRVFADLPKLLGETKECAVPLRVWLYPLDKLHSHYVKLQQEISIDLIRNVESVFESLRTTEMKCSDLLNDTPSLAFAGFHDKIMIMKQNCCNYKLSFMKKLGSLLPKIRSDTENETALIELLHDHEKCPFRKRDLEKWVKEKENESVIIKTLLRQLTDSGATVENNLDGILMDLNVENVVSYTFTSLECPDVLLTKQKAFLNPSTIENNSEDASVLSKKSGFTSDIKTTMRSNLIIFKNLMESKICKPAKYIVASKEIPNPGSCILIYENGSDEATCFTPPIKPAHPVTEQISGERVVLKVTPVCPATEELRLLYKTKEEKDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKNLTDATESILEKLSLIEDKCSKLMEGKSAVTFRAIRNKFEDMKRHCQTYKQDRIKSTIQSVQACEKDISALTDLLQAHHESPFNISDIVEWIRVKETESKAVEKILQQLLETGAEVNNSLDEYLLDVSFENVVCYTFTSLDLPDDLLSDQEDFLKPEMIRRNSDKKPNAVSETWLTGSTREKLKKQLEMFKELKCSYGSHSTKFLVISEDQTNHPGSCILLYENGSYKAKCFTPPIKPAEPIIEQVIGERVVLKVTSVCPATEELRLLYKTKEENDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKKLTDATESILEKLSLIEDKCSKLMEDKSAVTFRAIHNKFEDMRRHCQIYKQDLEIKITSTMQSVQTCEKDISALTDLLQAHHESPFNESDLIEWIRVKETESNAVEKILQQLLDSGTEVNNSLDEYLFDVSFENVVCYTFTSLDLPDNLLSKQGDFLKPEIMRRNSNKNPNSVSETWLTGSIRETMKKHLEIFNELKCSYGSHSSTFLVVYKSHTNHPGSCILIYENGSDKATCFTPPIKPASPVIEQIGEDIVVLKATPLCPATQELRLLYKKKEENDWKSQSVKTSGDEVFLTGLRPDTEYKMKYVALGKLNYTTDSDVIGVTTRVVSEKQCALPYSCNTNFTYFNSELLNSNSMQLNETGKYLQDKSEQKLSMTKEPRNNQPMSFDSSEEVKVCTVLAGKTNNCQTHFIASLRARIEHLTEVRSVDESDIVLIFCPIVSRAGTDIDAAIHRCYTNASKLTVLVVLHHTFDPEKTVSDSSKIVNRENLLTVDCLFYEDAGLLQCQRNSDAYDKSVNWLIAQGKKTGLKIYPKKSPDSQSNGRKFWR

Target ORF information:

RefSeq Version	XM_021476450.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476450.1

ORF Insert Sequence:

ATGGACTTGG GAGTGGGAGT TAACATTGTT GAAACAGCAG CTCTGGGGAG ACCCTTCAGG 
CTGGGGATGC TGTACGACTG CAGAAAAGAT GCATTTGTAC CAGGAATCAC ACTGTGGGAT 
GAAGAGCAAC TGCAGCAGAG CATAAAAATC CATCCTCAAA TTAAAACAGA TTTCTGTGTT 
ACAAATTCAG ACTTTATTGA GGCAAAATGC CAATTACTGA ACATTAATGA TTGTCTAAAA 
CTGAGTTTTT TAAGTGGACT CATCAATGTT AGTGGAGCAG CCAAGTATCT CAGTGACACC 
AAGAAATCCT TCAAACAGCA GAGACTGACC CTACATTATC ATTCAACCAC CAAACGTGAA 
GAACTGACTG TGAACCACTT GCCATCTGAA AATATAACCC ACTATGAAGT GTTTGAGAAT 
GATGCAGCGA CACATGTGGT GACAGCTGTG CTGTATGGGG CCAATGCTTG CTTTGTTTTT 
GACAGAGAAG CTTCATTAGT TGAAGACCAA AGCACTGTAG ACAGAGACGT AAAGGCTGCC 
TTAGAAAAAC TAAAAGGGAT TTCAGAAAGT ACACAGATTG ATCTGAGCTT GGATGACAAT 
CAAAAGACTG TTTTTGAGAA ATTCAGCTGC ACATTTTATG GTGATTTCCA GTTAAAATCT 
AATCCAGCCT CTTTTGAAGA AGCTCTGAGG GTTTTTGCTG ATCTTCCTAA ACTGCTGGGA 
GAAACCAAGG AGTGTGCAGT TCCACTGAGA GTGTGGCTTT ATCCACTGGA CAAACTGCAT 
TCACATTATG TGAAACTTCA ACAAGAAATC AGCATAGATC TAATCAGAAA TGTTGAATCA 
GTATTTGAGA GCTTAAGGAC CACTGAAATG AAATGCAGTG ATCTTCTGAA TGATACACCA 
TCTTTGGCTT TTGCAGGATT TCATGATAAA ATAATGATTA TGAAGCAAAA CTGCTGCAAC 
TATAAGCTGA GCTTCATGAA GAAACTTGGA TCGCTATTGC CAAAAATCCG TAGTGATACA 
GAAAATGAGA CAGCCCTGAT TGAGCTCCTC CATGATCATG AAAAATGTCC ATTCAGAAAA 
AGGGATCTTG AGAAATGGGT GAAAGAAAAA GAGAATGAAT CCGTCATTAT TAAAACTCTT 
CTCAGACAAT TGACTGATTC TGGAGCAACA GTGGAAAATA ATCTAGATGG AATTTTAATG 
GATCTTAATG TTGAAAATGT AGTAAGCTAC ACTTTCACAT CACTTGAGTG TCCAGATGTG 
CTCCTTACTA AACAAAAGGC TTTCCTGAAT CCATCAACAA TAGAAAACAA CAGTGAGGAT 
GCTTCTGTTT TAAGTAAAAA ATCTGGGTTC ACATCTGACA TCAAAACCAC AATGAGGAGC 
AACTTGATCA TCTTCAAAAA CTTGATGGAA TCAAAAATAT GTAAACCAGC CAAATATATT 
GTTGCATCGA AGGAAATACC AAATCCAGGT TCCTGCATTC TCATTTACGA AAATGGATCT 
GATGAAGCGA CATGCTTTAC TCCGCCAATA AAACCAGCTC ATCCAGTCAC TGAACAGATC 
AGTGGGGAAA GAGTGGTTTT AAAGGTGACA CCTGTGTGTC CTGCAACAGA AGAGCTCAGG 
TTACTGTACA AAACAAAAGA AGAGAAAGAC TGGAAATCTC AGTCTGTGAA AAAGAGTCAA 
GATACAGTTA TTCTGACTGA TCTCAGACCA GACACTGAGT ATGAGATGAA ATTTGCAGCC 
GTGGGGAAGC TGAACTACAC CATAGACAGT GATGTGATTC ACCTCAGAGT TATTGACAAA 
AACCTCACTG ATGCCACAGA GTCCATTTTA GAAAAGCTGA GCTTGATAGA AGACAAGTGC 
AGTAAACTGA TGGAGGGCAA GAGTGCAGTC ACATTTAGAG CAATTCGCAA CAAGTTTGAA 
GACATGAAGA GACACTGTCA GACTTACAAA CAAGACAGAA TTAAATCCAC AATCCAGTCA 
GTTCAAGCCT GTGAAAAAGA CATCAGTGCT CTGACAGACC TTCTACAAGC TCATCATGAG 
TCTCCATTTA ACATAAGTGA TATTGTGGAG TGGATCAGAG TGAAAGAAAC CGAATCAAAA 
GCAGTTGAGA AGATTCTTCA ACAGCTGCTG GAAACTGGAG CTGAAGTGAA CAATAGTTTA 
GATGAATATT TGTTGGATGT TAGTTTTGAG AATGTGGTGT GTTACACATT CACTTCTCTT 
GATCTGCCAG ACGACCTGCT TTCTGATCAA GAAGATTTCC TGAAACCTGA AATGATAAGG 
AGAAATTCTG ATAAGAAACC CAATGCTGTG TCTGAAACTT GGCTCACAGG AAGTACCAGA 
GAGAAATTGA AAAAACAATT AGAAATGTTT AAAGAGCTCA AATGTTCATA TGGCAGTCAC 
TCAACAAAGT TTTTGGTTAT ATCAGAAGAT CAGACAAACC ATCCAGGTTC CTGTATTCTC 
CTTTATGAAA ATGGATCTTA TAAAGCGAAA TGCTTTACTC CGCCAATAAA ACCAGCTGAG 
CCCATCATTG AACAGGTCAT CGGGGAAAGA GTGGTTTTGA AGGTGACATC TGTGTGTCCT 
GCAACAGAAG AGCTCAGGTT ACTGTACAAA ACAAAAGAAG AGAACGACTG GAAATCTCAG 
TCTGTGAAAA AGAGTCAAGA TACAGTTATT CTGACTGATC TCAGACCAGA CACTGAGTAT 
GAGATGAAAT TTGCAGCTGT GGGGAAGCTG AACTACACCA TAGACAGTGA TGTGATTCAC 
CTTAGAGTTA TTGACAAGAA ACTCACTGAT GCCACAGAGT CCATTTTAGA AAAGCTGAGC 
TTGATAGAAG ACAAGTGCAG TAAACTGATG GAGGACAAGA GTGCAGTCAC TTTTAGAGCA 
ATTCACAACA AGTTTGAAGA CATGAGAAGA CACTGTCAGA TCTACAAACA AGACTTAGAA 
ATTAAAATTA CATCCACAAT GCAGTCAGTT CAAACCTGTG AAAAAGACAT CAGTGCTCTG 
ACAGACCTTC TACAAGCTCA TCATGAGTCT CCATTTAATG AAAGTGATCT TATAGAGTGG 
ATCAGAGTGA AAGAAACCGA ATCAAATGCA GTTGAGAAGA TTCTTCAACA GCTGCTGGAC 
TCTGGAACTG AGGTGAACAA TAGTTTAGAT GAATATTTGT TTGATGTTAG TTTTGAGAAT 
GTGGTGTGTT ACACATTCAC TTCTCTTGAT CTGCCAGACA ACCTGCTTTC TAAACAAGGA 
GATTTCCTGA AACCTGAAAT AATGAGGAGA AATTCTAATA AGAACCCCAA TTCTGTGTCT 
GAAACTTGGC TCACAGGAAG TATCAGAGAG ACTATGAAGA AGCATTTGGA AATATTTAAT 
GAGCTCAAAT GTTCATATGG CAGTCACTCA TCAACATTTT TGGTTGTATA TAAAAGTCAC 
ACAAACCATC CAGGTTCCTG CATTCTTATT TATGAAAATG GATCTGATAA AGCGACATGC 
TTCACTCCTC CAATAAAACC AGCTAGTCCA GTCATTGAAC AGATTGGTGA GGATATAGTG 
GTTTTGAAGG CAACACCATT ATGTCCTGCA ACACAAGAGC TCAGGTTACT GTACAAAAAA 
AAAGAAGAGA ATGATTGGAA ATCTCAGTCT GTGAAAACGA GTGGAGATGA AGTTTTTCTG 
ACTGGTCTCA GACCTGACAC TGAGTATAAG ATGAAATATG TGGCCTTGGG GAAGCTGAAC 
TACACCACAG ACAGTGATGT CATCGGAGTT ACTACACGTG TGGTCAGTGA GAAACAGTGT 
GCCTTGCCTT ATTCATGCAA TACTAATTTC ACATATTTTA ACAGTGAATT ATTGAACAGT 
AACTCTATGC AGCTAAATGA GACTGGCAAA TATCTGCAGG ATAAATCTGA ACAAAAACTA 
TCGATGACAA AGGAGCCAAG GAACAACCAA CCAATGTCAT TTGATTCTTC AGAGGAGGTC 
AAAGTGTGCA CTGTTTTGGC TGGAAAGACC AATAACTGTC AAACACATTT TATTGCATCA 
CTGCGAGCCC GAATTGAGCA TCTGACGGAA GTTCGTTCAG TAGACGAGAG TGACATTGTT 
TTAATTTTCT GTCCAATTGT TTCTCGAGCT GGAACTGACA TTGATGCAGC CATTCACAGA 
TGCTATACAA ATGCCTCTAA GCTGACTGTT CTAGTGGTGC TTCACCACAC GTTTGACCCA 
GAGAAAACTG TATCAGACAG CAGCAAAATT GTCAACAGGG AGAATCTGCT CACAGTGGAC 
TGTCTGTTCT ATGAGGATGC AGGATTACTG CAGTGTCAGA GGAATTCAGA TGCATATGAC 
AAATCTGTCA ACTGGTTAAT AGCGCAGGGC AAAAAAACAG GTCTTAAAAT CTACCCAAAG 
AAATCACCTG ATAGTCAATC CAATGGTCGG AAATTCTGGA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100148510 ( XM_009301400.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299675.1 Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X5, mRNA.

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CloneID	ODa10353
Clone ID Related Accession (Same CDS sequence)	XM_021476449.1 , XM_021476450.1 , XM_009301400.2 , XM_009301401.2 , XM_009301400.3 , XM_009301401.3
Accession Version	XM_009301400.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein LOC100148510 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301400.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTGG GAGTGGGAGT TAACATTGTT GAAACAGCAG CTCTGGGGAG ACCCTTCAGG 
CTGGGGATGC TGTACGACTG CAGAAAAGAT GCATTTGTAC CAGGAATCAC ACTGTGGGAT 
GAAGAGCAAC TGCAGCAGAG CATAAAAATC CATCCTCAAA TTAAAACAGA TTTCTGTGTT 
ACAAATTCAG ACTTTATTGA GGCAAAATGC CAATTACTGA ACATTAATGA TTGTCTAAAA 
CTGAGTTTTT TAAGTGGACT CATCAATGTT AGTGGAGCAG CCAAGTATCT CAGTGACACC 
AAGAAATCCT TCAAACAGCA GAGACTGACC CTACATTATC ATTCAACCAC CAAACGTGAA 
GAACTGACTG TGAACCACTT GCCATCTGAA AATATAACCC ACTATGAAGT GTTTGAGAAT 
GATGCAGCGA CACATGTGGT GACAGCTGTG CTGTATGGGG CCAATGCTTG CTTTGTTTTT 
GACAGAGAAG CTTCATTAGT TGAAGACCAA AGCACTGTAG ACAGAGACGT AAAGGCTGCC 
TTAGAAAAAC TAAAAGGGAT TTCAGAAAGT ACACAGATTG ATCTGAGCTT GGATGACAAT 
CAAAAGACTG TTTTTGAGAA ATTCAGCTGC ACATTTTATG GTGATTTCCA GTTAAAATCT 
AATCCAGCCT CTTTTGAAGA AGCTCTGAGG GTTTTTGCTG ATCTTCCTAA ACTGCTGGGA 
GAAACCAAGG AGTGTGCAGT TCCACTGAGA GTGTGGCTTT ATCCACTGGA CAAACTGCAT 
TCACATTATG TGAAACTTCA ACAAGAAATC AGCATAGATC TAATCAGAAA TGTTGAATCA 
GTATTTGAGA GCTTAAGGAC CACTGAAATG AAATGCAGTG ATCTTCTGAA TGATACACCA 
TCTTTGGCTT TTGCAGGATT TCATGATAAA ATAATGATTA TGAAGCAAAA CTGCTGCAAC 
TATAAGCTGA GCTTCATGAA GAAACTTGGA TCGCTATTGC CAAAAATCCG TAGTGATACA 
GAAAATGAGA CAGCCCTGAT TGAGCTCCTC CATGATCATG AAAAATGTCC ATTCAGAAAA 
AGGGATCTTG AGAAATGGGT GAAAGAAAAA GAGAATGAAT CCGTCATTAT TAAAACTCTT 
CTCAGACAAT TGACTGATTC TGGAGCAACA GTGGAAAATA ATCTAGATGG AATTTTAATG 
GATCTTAATG TTGAAAATGT AGTAAGCTAC ACTTTCACAT CACTTGAGTG TCCAGATGTG 
CTCCTTACTA AACAAAAGGC TTTCCTGAAT CCATCAACAA TAGAAAACAA CAGTGAGGAT 
GCTTCTGTTT TAAGTAAAAA ATCTGGGTTC ACATCTGACA TCAAAACCAC AATGAGGAGC 
AACTTGATCA TCTTCAAAAA CTTGATGGAA TCAAAAATAT GTAAACCAGC CAAATATATT 
GTTGCATCGA AGGAAATACC AAATCCAGGT TCCTGCATTC TCATTTACGA AAATGGATCT 
GATGAAGCGA CATGCTTTAC TCCGCCAATA AAACCAGCTC ATCCAGTCAC TGAACAGATC 
AGTGGGGAAA GAGTGGTTTT AAAGGTGACA CCTGTGTGTC CTGCAACAGA AGAGCTCAGG 
TTACTGTACA AAACAAAAGA AGAGAAAGAC TGGAAATCTC AGTCTGTGAA AAAGAGTCAA 
GATACAGTTA TTCTGACTGA TCTCAGACCA GACACTGAGT ATGAGATGAA ATTTGCAGCC 
GTGGGGAAGC TGAACTACAC CATAGACAGT GATGTGATTC ACCTCAGAGT TATTGACAAA 
AACCTCACTG ATGCCACAGA GTCCATTTTA GAAAAGCTGA GCTTGATAGA AGACAAGTGC 
AGTAAACTGA TGGAGGGCAA GAGTGCAGTC ACATTTAGAG CAATTCGCAA CAAGTTTGAA 
GACATGAAGA GACACTGTCA GACTTACAAA CAAGACAGAA TTAAATCCAC AATCCAGTCA 
GTTCAAGCCT GTGAAAAAGA CATCAGTGCT CTGACAGACC TTCTACAAGC TCATCATGAG 
TCTCCATTTA ACATAAGTGA TATTGTGGAG TGGATCAGAG TGAAAGAAAC CGAATCAAAA 
GCAGTTGAGA AGATTCTTCA ACAGCTGCTG GAAACTGGAG CTGAAGTGAA CAATAGTTTA 
GATGAATATT TGTTGGATGT TAGTTTTGAG AATGTGGTGT GTTACACATT CACTTCTCTT 
GATCTGCCAG ACGACCTGCT TTCTGATCAA GAAGATTTCC TGAAACCTGA AATGATAAGG 
AGAAATTCTG ATAAGAAACC CAATGCTGTG TCTGAAACTT GGCTCACAGG AAGTACCAGA 
GAGAAATTGA AAAAACAATT AGAAATGTTT AAAGAGCTCA AATGTTCATA TGGCAGTCAC 
TCAACAAAGT TTTTGGTTAT ATCAGAAGAT CAGACAAACC ATCCAGGTTC CTGTATTCTC 
CTTTATGAAA ATGGATCTTA TAAAGCGAAA TGCTTTACTC CGCCAATAAA ACCAGCTGAG 
CCCATCATTG AACAGGTCAT CGGGGAAAGA GTGGTTTTGA AGGTGACATC TGTGTGTCCT 
GCAACAGAAG AGCTCAGGTT ACTGTACAAA ACAAAAGAAG AGAACGACTG GAAATCTCAG 
TCTGTGAAAA AGAGTCAAGA TACAGTTATT CTGACTGATC TCAGACCAGA CACTGAGTAT 
GAGATGAAAT TTGCAGCTGT GGGGAAGCTG AACTACACCA TAGACAGTGA TGTGATTCAC 
CTTAGAGTTA TTGACAAGAA ACTCACTGAT GCCACAGAGT CCATTTTAGA AAAGCTGAGC 
TTGATAGAAG ACAAGTGCAG TAAACTGATG GAGGACAAGA GTGCAGTCAC TTTTAGAGCA 
ATTCACAACA AGTTTGAAGA CATGAGAAGA CACTGTCAGA TCTACAAACA AGACTTAGAA 
ATTAAAATTA CATCCACAAT GCAGTCAGTT CAAACCTGTG AAAAAGACAT CAGTGCTCTG 
ACAGACCTTC TACAAGCTCA TCATGAGTCT CCATTTAATG AAAGTGATCT TATAGAGTGG 
ATCAGAGTGA AAGAAACCGA ATCAAATGCA GTTGAGAAGA TTCTTCAACA GCTGCTGGAC 
TCTGGAACTG AGGTGAACAA TAGTTTAGAT GAATATTTGT TTGATGTTAG TTTTGAGAAT 
GTGGTGTGTT ACACATTCAC TTCTCTTGAT CTGCCAGACA ACCTGCTTTC TAAACAAGGA 
GATTTCCTGA AACCTGAAAT AATGAGGAGA AATTCTAATA AGAACCCCAA TTCTGTGTCT 
GAAACTTGGC TCACAGGAAG TATCAGAGAG ACTATGAAGA AGCATTTGGA AATATTTAAT 
GAGCTCAAAT GTTCATATGG CAGTCACTCA TCAACATTTT TGGTTGTATA TAAAAGTCAC 
ACAAACCATC CAGGTTCCTG CATTCTTATT TATGAAAATG GATCTGATAA AGCGACATGC 
TTCACTCCTC CAATAAAACC AGCTAGTCCA GTCATTGAAC AGATTGGTGA GGATATAGTG 
GTTTTGAAGG CAACACCATT ATGTCCTGCA ACACAAGAGC TCAGGTTACT GTACAAAAAA 
AAAGAAGAGA ATGATTGGAA ATCTCAGTCT GTGAAAACGA GTGGAGATGA AGTTTTTCTG 
ACTGGTCTCA GACCTGACAC TGAGTATAAG ATGAAATATG TGGCCTTGGG GAAGCTGAAC 
TACACCACAG ACAGTGATGT CATCGGAGTT ACTACACGTG TGGTCAGTGA GAAACAGTGT 
GCCTTGCCTT ATTCATGCAA TACTAATTTC ACATATTTTA ACAGTGAATT ATTGAACAGT 
AACTCTATGC AGCTAAATGA GACTGGCAAA TATCTGCAGG ATAAATCTGA ACAAAAACTA 
TCGATGACAA AGGAGCCAAG GAACAACCAA CCAATGTCAT TTGATTCTTC AGAGGAGGTC 
AAAGTGTGCA CTGTTTTGGC TGGAAAGACC AATAACTGTC AAACACATTT TATTGCATCA 
CTGCGAGCCC GAATTGAGCA TCTGACGGAA GTTCGTTCAG TAGACGAGAG TGACATTGTT 
TTAATTTTCT GTCCAATTGT TTCTCGAGCT GGAACTGACA TTGATGCAGC CATTCACAGA 
TGCTATACAA ATGCCTCTAA GCTGACTGTT CTAGTGGTGC TTCACCACAC GTTTGACCCA 
GAGAAAACTG TATCAGACAG CAGCAAAATT GTCAACAGGG AGAATCTGCT CACAGTGGAC 
TGTCTGTTCT ATGAGGATGC AGGATTACTG CAGTGTCAGA GGAATTCAGA TGCATATGAC 
AAATCTGTCA ACTGGTTAAT AGCGCAGGGC AAAAAAACAG GTCTTAAAAT CTACCCAAAG 
AAATCACCTG ATAGTCAATC CAATGGTCGG AAATTCTGGA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299675.1
CDS	274..4698
Translation	MDLGVGVNIVETAALGRPFRLGMLYDCRKDAFVPGITLWDEEQLQQSIKIHPQIKTDFCVTNSDFIEAKCQLLNINDCLKLSFLSGLINVSGAAKYLSDTKKSFKQQRLTLHYHSTTKREELTVNHLPSENITHYEVFENDAATHVVTAVLYGANACFVFDREASLVEDQSTVDRDVKAALEKLKGISESTQIDLSLDDNQKTVFEKFSCTFYGDFQLKSNPASFEEALRVFADLPKLLGETKECAVPLRVWLYPLDKLHSHYVKLQQEISIDLIRNVESVFESLRTTEMKCSDLLNDTPSLAFAGFHDKIMIMKQNCCNYKLSFMKKLGSLLPKIRSDTENETALIELLHDHEKCPFRKRDLEKWVKEKENESVIIKTLLRQLTDSGATVENNLDGILMDLNVENVVSYTFTSLECPDVLLTKQKAFLNPSTIENNSEDASVLSKKSGFTSDIKTTMRSNLIIFKNLMESKICKPAKYIVASKEIPNPGSCILIYENGSDEATCFTPPIKPAHPVTEQISGERVVLKVTPVCPATEELRLLYKTKEEKDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKNLTDATESILEKLSLIEDKCSKLMEGKSAVTFRAIRNKFEDMKRHCQTYKQDRIKSTIQSVQACEKDISALTDLLQAHHESPFNISDIVEWIRVKETESKAVEKILQQLLETGAEVNNSLDEYLLDVSFENVVCYTFTSLDLPDDLLSDQEDFLKPEMIRRNSDKKPNAVSETWLTGSTREKLKKQLEMFKELKCSYGSHSTKFLVISEDQTNHPGSCILLYENGSYKAKCFTPPIKPAEPIIEQVIGERVVLKVTSVCPATEELRLLYKTKEENDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKKLTDATESILEKLSLIEDKCSKLMEDKSAVTFRAIHNKFEDMRRHCQIYKQDLEIKITSTMQSVQTCEKDISALTDLLQAHHESPFNESDLIEWIRVKETESNAVEKILQQLLDSGTEVNNSLDEYLFDVSFENVVCYTFTSLDLPDNLLSKQGDFLKPEIMRRNSNKNPNSVSETWLTGSIRETMKKHLEIFNELKCSYGSHSSTFLVVYKSHTNHPGSCILIYENGSDKATCFTPPIKPASPVIEQIGEDIVVLKATPLCPATQELRLLYKKKEENDWKSQSVKTSGDEVFLTGLRPDTEYKMKYVALGKLNYTTDSDVIGVTTRVVSEKQCALPYSCNTNFTYFNSELLNSNSMQLNETGKYLQDKSEQKLSMTKEPRNNQPMSFDSSEEVKVCTVLAGKTNNCQTHFIASLRARIEHLTEVRSVDESDIVLIFCPIVSRAGTDIDAAIHRCYTNASKLTVLVVLHHTFDPEKTVSDSSKIVNRENLLTVDCLFYEDAGLLQCQRNSDAYDKSVNWLIAQGKKTGLKIYPKKSPDSQSNGRKFWR

Target ORF information:

RefSeq Version	XM_009301400.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301400.2

ORF Insert Sequence:

ATGGACTTGG GAGTGGGAGT TAACATTGTT GAAACAGCAG CTCTGGGGAG ACCCTTCAGG 
CTGGGGATGC TGTACGACTG CAGAAAAGAT GCATTTGTAC CAGGAATCAC ACTGTGGGAT 
GAAGAGCAAC TGCAGCAGAG CATAAAAATC CATCCTCAAA TTAAAACAGA TTTCTGTGTT 
ACAAATTCAG ACTTTATTGA GGCAAAATGC CAATTACTGA ACATTAATGA TTGTCTAAAA 
CTGAGTTTTT TAAGTGGACT CATCAATGTT AGTGGAGCAG CCAAGTATCT CAGTGACACC 
AAGAAATCCT TCAAACAGCA GAGACTGACC CTACATTATC ATTCAACCAC CAAACGTGAA 
GAACTGACTG TGAACCACTT GCCATCTGAA AATATAACCC ACTATGAAGT GTTTGAGAAT 
GATGCAGCGA CACATGTGGT GACAGCTGTG CTGTATGGGG CCAATGCTTG CTTTGTTTTT 
GACAGAGAAG CTTCATTAGT TGAAGACCAA AGCACTGTAG ACAGAGACGT AAAGGCTGCC 
TTAGAAAAAC TAAAAGGGAT TTCAGAAAGT ACACAGATTG ATCTGAGCTT GGATGACAAT 
CAAAAGACTG TTTTTGAGAA ATTCAGCTGC ACATTTTATG GTGATTTCCA GTTAAAATCT 
AATCCAGCCT CTTTTGAAGA AGCTCTGAGG GTTTTTGCTG ATCTTCCTAA ACTGCTGGGA 
GAAACCAAGG AGTGTGCAGT TCCACTGAGA GTGTGGCTTT ATCCACTGGA CAAACTGCAT 
TCACATTATG TGAAACTTCA ACAAGAAATC AGCATAGATC TAATCAGAAA TGTTGAATCA 
GTATTTGAGA GCTTAAGGAC CACTGAAATG AAATGCAGTG ATCTTCTGAA TGATACACCA 
TCTTTGGCTT TTGCAGGATT TCATGATAAA ATAATGATTA TGAAGCAAAA CTGCTGCAAC 
TATAAGCTGA GCTTCATGAA GAAACTTGGA TCGCTATTGC CAAAAATCCG TAGTGATACA 
GAAAATGAGA CAGCCCTGAT TGAGCTCCTC CATGATCATG AAAAATGTCC ATTCAGAAAA 
AGGGATCTTG AGAAATGGGT GAAAGAAAAA GAGAATGAAT CCGTCATTAT TAAAACTCTT 
CTCAGACAAT TGACTGATTC TGGAGCAACA GTGGAAAATA ATCTAGATGG AATTTTAATG 
GATCTTAATG TTGAAAATGT AGTAAGCTAC ACTTTCACAT CACTTGAGTG TCCAGATGTG 
CTCCTTACTA AACAAAAGGC TTTCCTGAAT CCATCAACAA TAGAAAACAA CAGTGAGGAT 
GCTTCTGTTT TAAGTAAAAA ATCTGGGTTC ACATCTGACA TCAAAACCAC AATGAGGAGC 
AACTTGATCA TCTTCAAAAA CTTGATGGAA TCAAAAATAT GTAAACCAGC CAAATATATT 
GTTGCATCGA AGGAAATACC AAATCCAGGT TCCTGCATTC TCATTTACGA AAATGGATCT 
GATGAAGCGA CATGCTTTAC TCCGCCAATA AAACCAGCTC ATCCAGTCAC TGAACAGATC 
AGTGGGGAAA GAGTGGTTTT AAAGGTGACA CCTGTGTGTC CTGCAACAGA AGAGCTCAGG 
TTACTGTACA AAACAAAAGA AGAGAAAGAC TGGAAATCTC AGTCTGTGAA AAAGAGTCAA 
GATACAGTTA TTCTGACTGA TCTCAGACCA GACACTGAGT ATGAGATGAA ATTTGCAGCC 
GTGGGGAAGC TGAACTACAC CATAGACAGT GATGTGATTC ACCTCAGAGT TATTGACAAA 
AACCTCACTG ATGCCACAGA GTCCATTTTA GAAAAGCTGA GCTTGATAGA AGACAAGTGC 
AGTAAACTGA TGGAGGGCAA GAGTGCAGTC ACATTTAGAG CAATTCGCAA CAAGTTTGAA 
GACATGAAGA GACACTGTCA GACTTACAAA CAAGACAGAA TTAAATCCAC AATCCAGTCA 
GTTCAAGCCT GTGAAAAAGA CATCAGTGCT CTGACAGACC TTCTACAAGC TCATCATGAG 
TCTCCATTTA ACATAAGTGA TATTGTGGAG TGGATCAGAG TGAAAGAAAC CGAATCAAAA 
GCAGTTGAGA AGATTCTTCA ACAGCTGCTG GAAACTGGAG CTGAAGTGAA CAATAGTTTA 
GATGAATATT TGTTGGATGT TAGTTTTGAG AATGTGGTGT GTTACACATT CACTTCTCTT 
GATCTGCCAG ACGACCTGCT TTCTGATCAA GAAGATTTCC TGAAACCTGA AATGATAAGG 
AGAAATTCTG ATAAGAAACC CAATGCTGTG TCTGAAACTT GGCTCACAGG AAGTACCAGA 
GAGAAATTGA AAAAACAATT AGAAATGTTT AAAGAGCTCA AATGTTCATA TGGCAGTCAC 
TCAACAAAGT TTTTGGTTAT ATCAGAAGAT CAGACAAACC ATCCAGGTTC CTGTATTCTC 
CTTTATGAAA ATGGATCTTA TAAAGCGAAA TGCTTTACTC CGCCAATAAA ACCAGCTGAG 
CCCATCATTG AACAGGTCAT CGGGGAAAGA GTGGTTTTGA AGGTGACATC TGTGTGTCCT 
GCAACAGAAG AGCTCAGGTT ACTGTACAAA ACAAAAGAAG AGAACGACTG GAAATCTCAG 
TCTGTGAAAA AGAGTCAAGA TACAGTTATT CTGACTGATC TCAGACCAGA CACTGAGTAT 
GAGATGAAAT TTGCAGCTGT GGGGAAGCTG AACTACACCA TAGACAGTGA TGTGATTCAC 
CTTAGAGTTA TTGACAAGAA ACTCACTGAT GCCACAGAGT CCATTTTAGA AAAGCTGAGC 
TTGATAGAAG ACAAGTGCAG TAAACTGATG GAGGACAAGA GTGCAGTCAC TTTTAGAGCA 
ATTCACAACA AGTTTGAAGA CATGAGAAGA CACTGTCAGA TCTACAAACA AGACTTAGAA 
ATTAAAATTA CATCCACAAT GCAGTCAGTT CAAACCTGTG AAAAAGACAT CAGTGCTCTG 
ACAGACCTTC TACAAGCTCA TCATGAGTCT CCATTTAATG AAAGTGATCT TATAGAGTGG 
ATCAGAGTGA AAGAAACCGA ATCAAATGCA GTTGAGAAGA TTCTTCAACA GCTGCTGGAC 
TCTGGAACTG AGGTGAACAA TAGTTTAGAT GAATATTTGT TTGATGTTAG TTTTGAGAAT 
GTGGTGTGTT ACACATTCAC TTCTCTTGAT CTGCCAGACA ACCTGCTTTC TAAACAAGGA 
GATTTCCTGA AACCTGAAAT AATGAGGAGA AATTCTAATA AGAACCCCAA TTCTGTGTCT 
GAAACTTGGC TCACAGGAAG TATCAGAGAG ACTATGAAGA AGCATTTGGA AATATTTAAT 
GAGCTCAAAT GTTCATATGG CAGTCACTCA TCAACATTTT TGGTTGTATA TAAAAGTCAC 
ACAAACCATC CAGGTTCCTG CATTCTTATT TATGAAAATG GATCTGATAA AGCGACATGC 
TTCACTCCTC CAATAAAACC AGCTAGTCCA GTCATTGAAC AGATTGGTGA GGATATAGTG 
GTTTTGAAGG CAACACCATT ATGTCCTGCA ACACAAGAGC TCAGGTTACT GTACAAAAAA 
AAAGAAGAGA ATGATTGGAA ATCTCAGTCT GTGAAAACGA GTGGAGATGA AGTTTTTCTG 
ACTGGTCTCA GACCTGACAC TGAGTATAAG ATGAAATATG TGGCCTTGGG GAAGCTGAAC 
TACACCACAG ACAGTGATGT CATCGGAGTT ACTACACGTG TGGTCAGTGA GAAACAGTGT 
GCCTTGCCTT ATTCATGCAA TACTAATTTC ACATATTTTA ACAGTGAATT ATTGAACAGT 
AACTCTATGC AGCTAAATGA GACTGGCAAA TATCTGCAGG ATAAATCTGA ACAAAAACTA 
TCGATGACAA AGGAGCCAAG GAACAACCAA CCAATGTCAT TTGATTCTTC AGAGGAGGTC 
AAAGTGTGCA CTGTTTTGGC TGGAAAGACC AATAACTGTC AAACACATTT TATTGCATCA 
CTGCGAGCCC GAATTGAGCA TCTGACGGAA GTTCGTTCAG TAGACGAGAG TGACATTGTT 
TTAATTTTCT GTCCAATTGT TTCTCGAGCT GGAACTGACA TTGATGCAGC CATTCACAGA 
TGCTATACAA ATGCCTCTAA GCTGACTGTT CTAGTGGTGC TTCACCACAC GTTTGACCCA 
GAGAAAACTG TATCAGACAG CAGCAAAATT GTCAACAGGG AGAATCTGCT CACAGTGGAC 
TGTCTGTTCT ATGAGGATGC AGGATTACTG CAGTGTCAGA GGAATTCAGA TGCATATGAC 
AAATCTGTCA ACTGGTTAAT AGCGCAGGGC AAAAAAACAG GTCTTAAAAT CTACCCAAAG 
AAATCACCTG ATAGTCAATC CAATGGTCGG AAATTCTGGA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100148510 ( XM_009301401.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299676.1 Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa10353
Clone ID Related Accession (Same CDS sequence)	XM_021476449.1 , XM_021476450.1 , XM_009301400.2 , XM_009301401.2 , XM_009301400.3 , XM_009301401.3
Accession Version	XM_009301401.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein LOC100148510 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301401.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTGG GAGTGGGAGT TAACATTGTT GAAACAGCAG CTCTGGGGAG ACCCTTCAGG 
CTGGGGATGC TGTACGACTG CAGAAAAGAT GCATTTGTAC CAGGAATCAC ACTGTGGGAT 
GAAGAGCAAC TGCAGCAGAG CATAAAAATC CATCCTCAAA TTAAAACAGA TTTCTGTGTT 
ACAAATTCAG ACTTTATTGA GGCAAAATGC CAATTACTGA ACATTAATGA TTGTCTAAAA 
CTGAGTTTTT TAAGTGGACT CATCAATGTT AGTGGAGCAG CCAAGTATCT CAGTGACACC 
AAGAAATCCT TCAAACAGCA GAGACTGACC CTACATTATC ATTCAACCAC CAAACGTGAA 
GAACTGACTG TGAACCACTT GCCATCTGAA AATATAACCC ACTATGAAGT GTTTGAGAAT 
GATGCAGCGA CACATGTGGT GACAGCTGTG CTGTATGGGG CCAATGCTTG CTTTGTTTTT 
GACAGAGAAG CTTCATTAGT TGAAGACCAA AGCACTGTAG ACAGAGACGT AAAGGCTGCC 
TTAGAAAAAC TAAAAGGGAT TTCAGAAAGT ACACAGATTG ATCTGAGCTT GGATGACAAT 
CAAAAGACTG TTTTTGAGAA ATTCAGCTGC ACATTTTATG GTGATTTCCA GTTAAAATCT 
AATCCAGCCT CTTTTGAAGA AGCTCTGAGG GTTTTTGCTG ATCTTCCTAA ACTGCTGGGA 
GAAACCAAGG AGTGTGCAGT TCCACTGAGA GTGTGGCTTT ATCCACTGGA CAAACTGCAT 
TCACATTATG TGAAACTTCA ACAAGAAATC AGCATAGATC TAATCAGAAA TGTTGAATCA 
GTATTTGAGA GCTTAAGGAC CACTGAAATG AAATGCAGTG ATCTTCTGAA TGATACACCA 
TCTTTGGCTT TTGCAGGATT TCATGATAAA ATAATGATTA TGAAGCAAAA CTGCTGCAAC 
TATAAGCTGA GCTTCATGAA GAAACTTGGA TCGCTATTGC CAAAAATCCG TAGTGATACA 
GAAAATGAGA CAGCCCTGAT TGAGCTCCTC CATGATCATG AAAAATGTCC ATTCAGAAAA 
AGGGATCTTG AGAAATGGGT GAAAGAAAAA GAGAATGAAT CCGTCATTAT TAAAACTCTT 
CTCAGACAAT TGACTGATTC TGGAGCAACA GTGGAAAATA ATCTAGATGG AATTTTAATG 
GATCTTAATG TTGAAAATGT AGTAAGCTAC ACTTTCACAT CACTTGAGTG TCCAGATGTG 
CTCCTTACTA AACAAAAGGC TTTCCTGAAT CCATCAACAA TAGAAAACAA CAGTGAGGAT 
GCTTCTGTTT TAAGTAAAAA ATCTGGGTTC ACATCTGACA TCAAAACCAC AATGAGGAGC 
AACTTGATCA TCTTCAAAAA CTTGATGGAA TCAAAAATAT GTAAACCAGC CAAATATATT 
GTTGCATCGA AGGAAATACC AAATCCAGGT TCCTGCATTC TCATTTACGA AAATGGATCT 
GATGAAGCGA CATGCTTTAC TCCGCCAATA AAACCAGCTC ATCCAGTCAC TGAACAGATC 
AGTGGGGAAA GAGTGGTTTT AAAGGTGACA CCTGTGTGTC CTGCAACAGA AGAGCTCAGG 
TTACTGTACA AAACAAAAGA AGAGAAAGAC TGGAAATCTC AGTCTGTGAA AAAGAGTCAA 
GATACAGTTA TTCTGACTGA TCTCAGACCA GACACTGAGT ATGAGATGAA ATTTGCAGCC 
GTGGGGAAGC TGAACTACAC CATAGACAGT GATGTGATTC ACCTCAGAGT TATTGACAAA 
AACCTCACTG ATGCCACAGA GTCCATTTTA GAAAAGCTGA GCTTGATAGA AGACAAGTGC 
AGTAAACTGA TGGAGGGCAA GAGTGCAGTC ACATTTAGAG CAATTCGCAA CAAGTTTGAA 
GACATGAAGA GACACTGTCA GACTTACAAA CAAGACAGAA TTAAATCCAC AATCCAGTCA 
GTTCAAGCCT GTGAAAAAGA CATCAGTGCT CTGACAGACC TTCTACAAGC TCATCATGAG 
TCTCCATTTA ACATAAGTGA TATTGTGGAG TGGATCAGAG TGAAAGAAAC CGAATCAAAA 
GCAGTTGAGA AGATTCTTCA ACAGCTGCTG GAAACTGGAG CTGAAGTGAA CAATAGTTTA 
GATGAATATT TGTTGGATGT TAGTTTTGAG AATGTGGTGT GTTACACATT CACTTCTCTT 
GATCTGCCAG ACGACCTGCT TTCTGATCAA GAAGATTTCC TGAAACCTGA AATGATAAGG 
AGAAATTCTG ATAAGAAACC CAATGCTGTG TCTGAAACTT GGCTCACAGG AAGTACCAGA 
GAGAAATTGA AAAAACAATT AGAAATGTTT AAAGAGCTCA AATGTTCATA TGGCAGTCAC 
TCAACAAAGT TTTTGGTTAT ATCAGAAGAT CAGACAAACC ATCCAGGTTC CTGTATTCTC 
CTTTATGAAA ATGGATCTTA TAAAGCGAAA TGCTTTACTC CGCCAATAAA ACCAGCTGAG 
CCCATCATTG AACAGGTCAT CGGGGAAAGA GTGGTTTTGA AGGTGACATC TGTGTGTCCT 
GCAACAGAAG AGCTCAGGTT ACTGTACAAA ACAAAAGAAG AGAACGACTG GAAATCTCAG 
TCTGTGAAAA AGAGTCAAGA TACAGTTATT CTGACTGATC TCAGACCAGA CACTGAGTAT 
GAGATGAAAT TTGCAGCTGT GGGGAAGCTG AACTACACCA TAGACAGTGA TGTGATTCAC 
CTTAGAGTTA TTGACAAGAA ACTCACTGAT GCCACAGAGT CCATTTTAGA AAAGCTGAGC 
TTGATAGAAG ACAAGTGCAG TAAACTGATG GAGGACAAGA GTGCAGTCAC TTTTAGAGCA 
ATTCACAACA AGTTTGAAGA CATGAGAAGA CACTGTCAGA TCTACAAACA AGACTTAGAA 
ATTAAAATTA CATCCACAAT GCAGTCAGTT CAAACCTGTG AAAAAGACAT CAGTGCTCTG 
ACAGACCTTC TACAAGCTCA TCATGAGTCT CCATTTAATG AAAGTGATCT TATAGAGTGG 
ATCAGAGTGA AAGAAACCGA ATCAAATGCA GTTGAGAAGA TTCTTCAACA GCTGCTGGAC 
TCTGGAACTG AGGTGAACAA TAGTTTAGAT GAATATTTGT TTGATGTTAG TTTTGAGAAT 
GTGGTGTGTT ACACATTCAC TTCTCTTGAT CTGCCAGACA ACCTGCTTTC TAAACAAGGA 
GATTTCCTGA AACCTGAAAT AATGAGGAGA AATTCTAATA AGAACCCCAA TTCTGTGTCT 
GAAACTTGGC TCACAGGAAG TATCAGAGAG ACTATGAAGA AGCATTTGGA AATATTTAAT 
GAGCTCAAAT GTTCATATGG CAGTCACTCA TCAACATTTT TGGTTGTATA TAAAAGTCAC 
ACAAACCATC CAGGTTCCTG CATTCTTATT TATGAAAATG GATCTGATAA AGCGACATGC 
TTCACTCCTC CAATAAAACC AGCTAGTCCA GTCATTGAAC AGATTGGTGA GGATATAGTG 
GTTTTGAAGG CAACACCATT ATGTCCTGCA ACACAAGAGC TCAGGTTACT GTACAAAAAA 
AAAGAAGAGA ATGATTGGAA ATCTCAGTCT GTGAAAACGA GTGGAGATGA AGTTTTTCTG 
ACTGGTCTCA GACCTGACAC TGAGTATAAG ATGAAATATG TGGCCTTGGG GAAGCTGAAC 
TACACCACAG ACAGTGATGT CATCGGAGTT ACTACACGTG TGGTCAGTGA GAAACAGTGT 
GCCTTGCCTT ATTCATGCAA TACTAATTTC ACATATTTTA ACAGTGAATT ATTGAACAGT 
AACTCTATGC AGCTAAATGA GACTGGCAAA TATCTGCAGG ATAAATCTGA ACAAAAACTA 
TCGATGACAA AGGAGCCAAG GAACAACCAA CCAATGTCAT TTGATTCTTC AGAGGAGGTC 
AAAGTGTGCA CTGTTTTGGC TGGAAAGACC AATAACTGTC AAACACATTT TATTGCATCA 
CTGCGAGCCC GAATTGAGCA TCTGACGGAA GTTCGTTCAG TAGACGAGAG TGACATTGTT 
TTAATTTTCT GTCCAATTGT TTCTCGAGCT GGAACTGACA TTGATGCAGC CATTCACAGA 
TGCTATACAA ATGCCTCTAA GCTGACTGTT CTAGTGGTGC TTCACCACAC GTTTGACCCA 
GAGAAAACTG TATCAGACAG CAGCAAAATT GTCAACAGGG AGAATCTGCT CACAGTGGAC 
TGTCTGTTCT ATGAGGATGC AGGATTACTG CAGTGTCAGA GGAATTCAGA TGCATATGAC 
AAATCTGTCA ACTGGTTAAT AGCGCAGGGC AAAAAAACAG GTCTTAAAAT CTACCCAAAG 
AAATCACCTG ATAGTCAATC CAATGGTCGG AAATTCTGGA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299676.1
CDS	362..4786
Translation	MDLGVGVNIVETAALGRPFRLGMLYDCRKDAFVPGITLWDEEQLQQSIKIHPQIKTDFCVTNSDFIEAKCQLLNINDCLKLSFLSGLINVSGAAKYLSDTKKSFKQQRLTLHYHSTTKREELTVNHLPSENITHYEVFENDAATHVVTAVLYGANACFVFDREASLVEDQSTVDRDVKAALEKLKGISESTQIDLSLDDNQKTVFEKFSCTFYGDFQLKSNPASFEEALRVFADLPKLLGETKECAVPLRVWLYPLDKLHSHYVKLQQEISIDLIRNVESVFESLRTTEMKCSDLLNDTPSLAFAGFHDKIMIMKQNCCNYKLSFMKKLGSLLPKIRSDTENETALIELLHDHEKCPFRKRDLEKWVKEKENESVIIKTLLRQLTDSGATVENNLDGILMDLNVENVVSYTFTSLECPDVLLTKQKAFLNPSTIENNSEDASVLSKKSGFTSDIKTTMRSNLIIFKNLMESKICKPAKYIVASKEIPNPGSCILIYENGSDEATCFTPPIKPAHPVTEQISGERVVLKVTPVCPATEELRLLYKTKEEKDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKNLTDATESILEKLSLIEDKCSKLMEGKSAVTFRAIRNKFEDMKRHCQTYKQDRIKSTIQSVQACEKDISALTDLLQAHHESPFNISDIVEWIRVKETESKAVEKILQQLLETGAEVNNSLDEYLLDVSFENVVCYTFTSLDLPDDLLSDQEDFLKPEMIRRNSDKKPNAVSETWLTGSTREKLKKQLEMFKELKCSYGSHSTKFLVISEDQTNHPGSCILLYENGSYKAKCFTPPIKPAEPIIEQVIGERVVLKVTSVCPATEELRLLYKTKEENDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKKLTDATESILEKLSLIEDKCSKLMEDKSAVTFRAIHNKFEDMRRHCQIYKQDLEIKITSTMQSVQTCEKDISALTDLLQAHHESPFNESDLIEWIRVKETESNAVEKILQQLLDSGTEVNNSLDEYLFDVSFENVVCYTFTSLDLPDNLLSKQGDFLKPEIMRRNSNKNPNSVSETWLTGSIRETMKKHLEIFNELKCSYGSHSSTFLVVYKSHTNHPGSCILIYENGSDKATCFTPPIKPASPVIEQIGEDIVVLKATPLCPATQELRLLYKKKEENDWKSQSVKTSGDEVFLTGLRPDTEYKMKYVALGKLNYTTDSDVIGVTTRVVSEKQCALPYSCNTNFTYFNSELLNSNSMQLNETGKYLQDKSEQKLSMTKEPRNNQPMSFDSSEEVKVCTVLAGKTNNCQTHFIASLRARIEHLTEVRSVDESDIVLIFCPIVSRAGTDIDAAIHRCYTNASKLTVLVVLHHTFDPEKTVSDSSKIVNRENLLTVDCLFYEDAGLLQCQRNSDAYDKSVNWLIAQGKKTGLKIYPKKSPDSQSNGRKFWR

Target ORF information:

RefSeq Version	XM_009301401.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301401.2

ORF Insert Sequence:

ATGGACTTGG GAGTGGGAGT TAACATTGTT GAAACAGCAG CTCTGGGGAG ACCCTTCAGG 
CTGGGGATGC TGTACGACTG CAGAAAAGAT GCATTTGTAC CAGGAATCAC ACTGTGGGAT 
GAAGAGCAAC TGCAGCAGAG CATAAAAATC CATCCTCAAA TTAAAACAGA TTTCTGTGTT 
ACAAATTCAG ACTTTATTGA GGCAAAATGC CAATTACTGA ACATTAATGA TTGTCTAAAA 
CTGAGTTTTT TAAGTGGACT CATCAATGTT AGTGGAGCAG CCAAGTATCT CAGTGACACC 
AAGAAATCCT TCAAACAGCA GAGACTGACC CTACATTATC ATTCAACCAC CAAACGTGAA 
GAACTGACTG TGAACCACTT GCCATCTGAA AATATAACCC ACTATGAAGT GTTTGAGAAT 
GATGCAGCGA CACATGTGGT GACAGCTGTG CTGTATGGGG CCAATGCTTG CTTTGTTTTT 
GACAGAGAAG CTTCATTAGT TGAAGACCAA AGCACTGTAG ACAGAGACGT AAAGGCTGCC 
TTAGAAAAAC TAAAAGGGAT TTCAGAAAGT ACACAGATTG ATCTGAGCTT GGATGACAAT 
CAAAAGACTG TTTTTGAGAA ATTCAGCTGC ACATTTTATG GTGATTTCCA GTTAAAATCT 
AATCCAGCCT CTTTTGAAGA AGCTCTGAGG GTTTTTGCTG ATCTTCCTAA ACTGCTGGGA 
GAAACCAAGG AGTGTGCAGT TCCACTGAGA GTGTGGCTTT ATCCACTGGA CAAACTGCAT 
TCACATTATG TGAAACTTCA ACAAGAAATC AGCATAGATC TAATCAGAAA TGTTGAATCA 
GTATTTGAGA GCTTAAGGAC CACTGAAATG AAATGCAGTG ATCTTCTGAA TGATACACCA 
TCTTTGGCTT TTGCAGGATT TCATGATAAA ATAATGATTA TGAAGCAAAA CTGCTGCAAC 
TATAAGCTGA GCTTCATGAA GAAACTTGGA TCGCTATTGC CAAAAATCCG TAGTGATACA 
GAAAATGAGA CAGCCCTGAT TGAGCTCCTC CATGATCATG AAAAATGTCC ATTCAGAAAA 
AGGGATCTTG AGAAATGGGT GAAAGAAAAA GAGAATGAAT CCGTCATTAT TAAAACTCTT 
CTCAGACAAT TGACTGATTC TGGAGCAACA GTGGAAAATA ATCTAGATGG AATTTTAATG 
GATCTTAATG TTGAAAATGT AGTAAGCTAC ACTTTCACAT CACTTGAGTG TCCAGATGTG 
CTCCTTACTA AACAAAAGGC TTTCCTGAAT CCATCAACAA TAGAAAACAA CAGTGAGGAT 
GCTTCTGTTT TAAGTAAAAA ATCTGGGTTC ACATCTGACA TCAAAACCAC AATGAGGAGC 
AACTTGATCA TCTTCAAAAA CTTGATGGAA TCAAAAATAT GTAAACCAGC CAAATATATT 
GTTGCATCGA AGGAAATACC AAATCCAGGT TCCTGCATTC TCATTTACGA AAATGGATCT 
GATGAAGCGA CATGCTTTAC TCCGCCAATA AAACCAGCTC ATCCAGTCAC TGAACAGATC 
AGTGGGGAAA GAGTGGTTTT AAAGGTGACA CCTGTGTGTC CTGCAACAGA AGAGCTCAGG 
TTACTGTACA AAACAAAAGA AGAGAAAGAC TGGAAATCTC AGTCTGTGAA AAAGAGTCAA 
GATACAGTTA TTCTGACTGA TCTCAGACCA GACACTGAGT ATGAGATGAA ATTTGCAGCC 
GTGGGGAAGC TGAACTACAC CATAGACAGT GATGTGATTC ACCTCAGAGT TATTGACAAA 
AACCTCACTG ATGCCACAGA GTCCATTTTA GAAAAGCTGA GCTTGATAGA AGACAAGTGC 
AGTAAACTGA TGGAGGGCAA GAGTGCAGTC ACATTTAGAG CAATTCGCAA CAAGTTTGAA 
GACATGAAGA GACACTGTCA GACTTACAAA CAAGACAGAA TTAAATCCAC AATCCAGTCA 
GTTCAAGCCT GTGAAAAAGA CATCAGTGCT CTGACAGACC TTCTACAAGC TCATCATGAG 
TCTCCATTTA ACATAAGTGA TATTGTGGAG TGGATCAGAG TGAAAGAAAC CGAATCAAAA 
GCAGTTGAGA AGATTCTTCA ACAGCTGCTG GAAACTGGAG CTGAAGTGAA CAATAGTTTA 
GATGAATATT TGTTGGATGT TAGTTTTGAG AATGTGGTGT GTTACACATT CACTTCTCTT 
GATCTGCCAG ACGACCTGCT TTCTGATCAA GAAGATTTCC TGAAACCTGA AATGATAAGG 
AGAAATTCTG ATAAGAAACC CAATGCTGTG TCTGAAACTT GGCTCACAGG AAGTACCAGA 
GAGAAATTGA AAAAACAATT AGAAATGTTT AAAGAGCTCA AATGTTCATA TGGCAGTCAC 
TCAACAAAGT TTTTGGTTAT ATCAGAAGAT CAGACAAACC ATCCAGGTTC CTGTATTCTC 
CTTTATGAAA ATGGATCTTA TAAAGCGAAA TGCTTTACTC CGCCAATAAA ACCAGCTGAG 
CCCATCATTG AACAGGTCAT CGGGGAAAGA GTGGTTTTGA AGGTGACATC TGTGTGTCCT 
GCAACAGAAG AGCTCAGGTT ACTGTACAAA ACAAAAGAAG AGAACGACTG GAAATCTCAG 
TCTGTGAAAA AGAGTCAAGA TACAGTTATT CTGACTGATC TCAGACCAGA CACTGAGTAT 
GAGATGAAAT TTGCAGCTGT GGGGAAGCTG AACTACACCA TAGACAGTGA TGTGATTCAC 
CTTAGAGTTA TTGACAAGAA ACTCACTGAT GCCACAGAGT CCATTTTAGA AAAGCTGAGC 
TTGATAGAAG ACAAGTGCAG TAAACTGATG GAGGACAAGA GTGCAGTCAC TTTTAGAGCA 
ATTCACAACA AGTTTGAAGA CATGAGAAGA CACTGTCAGA TCTACAAACA AGACTTAGAA 
ATTAAAATTA CATCCACAAT GCAGTCAGTT CAAACCTGTG AAAAAGACAT CAGTGCTCTG 
ACAGACCTTC TACAAGCTCA TCATGAGTCT CCATTTAATG AAAGTGATCT TATAGAGTGG 
ATCAGAGTGA AAGAAACCGA ATCAAATGCA GTTGAGAAGA TTCTTCAACA GCTGCTGGAC 
TCTGGAACTG AGGTGAACAA TAGTTTAGAT GAATATTTGT TTGATGTTAG TTTTGAGAAT 
GTGGTGTGTT ACACATTCAC TTCTCTTGAT CTGCCAGACA ACCTGCTTTC TAAACAAGGA 
GATTTCCTGA AACCTGAAAT AATGAGGAGA AATTCTAATA AGAACCCCAA TTCTGTGTCT 
GAAACTTGGC TCACAGGAAG TATCAGAGAG ACTATGAAGA AGCATTTGGA AATATTTAAT 
GAGCTCAAAT GTTCATATGG CAGTCACTCA TCAACATTTT TGGTTGTATA TAAAAGTCAC 
ACAAACCATC CAGGTTCCTG CATTCTTATT TATGAAAATG GATCTGATAA AGCGACATGC 
TTCACTCCTC CAATAAAACC AGCTAGTCCA GTCATTGAAC AGATTGGTGA GGATATAGTG 
GTTTTGAAGG CAACACCATT ATGTCCTGCA ACACAAGAGC TCAGGTTACT GTACAAAAAA 
AAAGAAGAGA ATGATTGGAA ATCTCAGTCT GTGAAAACGA GTGGAGATGA AGTTTTTCTG 
ACTGGTCTCA GACCTGACAC TGAGTATAAG ATGAAATATG TGGCCTTGGG GAAGCTGAAC 
TACACCACAG ACAGTGATGT CATCGGAGTT ACTACACGTG TGGTCAGTGA GAAACAGTGT 
GCCTTGCCTT ATTCATGCAA TACTAATTTC ACATATTTTA ACAGTGAATT ATTGAACAGT 
AACTCTATGC AGCTAAATGA GACTGGCAAA TATCTGCAGG ATAAATCTGA ACAAAAACTA 
TCGATGACAA AGGAGCCAAG GAACAACCAA CCAATGTCAT TTGATTCTTC AGAGGAGGTC 
AAAGTGTGCA CTGTTTTGGC TGGAAAGACC AATAACTGTC AAACACATTT TATTGCATCA 
CTGCGAGCCC GAATTGAGCA TCTGACGGAA GTTCGTTCAG TAGACGAGAG TGACATTGTT 
TTAATTTTCT GTCCAATTGT TTCTCGAGCT GGAACTGACA TTGATGCAGC CATTCACAGA 
TGCTATACAA ATGCCTCTAA GCTGACTGTT CTAGTGGTGC TTCACCACAC GTTTGACCCA 
GAGAAAACTG TATCAGACAG CAGCAAAATT GTCAACAGGG AGAATCTGCT CACAGTGGAC 
TGTCTGTTCT ATGAGGATGC AGGATTACTG CAGTGTCAGA GGAATTCAGA TGCATATGAC 
AAATCTGTCA ACTGGTTAAT AGCGCAGGGC AAAAAAACAG GTCTTAAAAT CTACCCAAAG 
AAATCACCTG ATAGTCAATC CAATGGTCGG AAATTCTGGA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100148510 ( XM_009301400.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299675.1 Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa10353
Clone ID Related Accession (Same CDS sequence)	XM_021476449.1 , XM_021476450.1 , XM_009301400.2 , XM_009301401.2 , XM_009301400.3 , XM_009301401.3
Accession Version	XM_009301400.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC100148510 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301400.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTGG GAGTGGGAGT TAACATTGTT GAAACAGCAG CTCTGGGGAG ACCCTTCAGG 
CTGGGGATGC TGTACGACTG CAGAAAAGAT GCATTTGTAC CAGGAATCAC ACTGTGGGAT 
GAAGAGCAAC TGCAGCAGAG CATAAAAATC CATCCTCAAA TTAAAACAGA TTTCTGTGTT 
ACAAATTCAG ACTTTATTGA GGCAAAATGC CAATTACTGA ACATTAATGA TTGTCTAAAA 
CTGAGTTTTT TAAGTGGACT CATCAATGTT AGTGGAGCAG CCAAGTATCT CAGTGACACC 
AAGAAATCCT TCAAACAGCA GAGACTGACC CTACATTATC ATTCAACCAC CAAACGTGAA 
GAACTGACTG TGAACCACTT GCCATCTGAA AATATAACCC ACTATGAAGT GTTTGAGAAT 
GATGCAGCGA CACATGTGGT GACAGCTGTG CTGTATGGGG CCAATGCTTG CTTTGTTTTT 
GACAGAGAAG CTTCATTAGT TGAAGACCAA AGCACTGTAG ACAGAGACGT AAAGGCTGCC 
TTAGAAAAAC TAAAAGGGAT TTCAGAAAGT ACACAGATTG ATCTGAGCTT GGATGACAAT 
CAAAAGACTG TTTTTGAGAA ATTCAGCTGC ACATTTTATG GTGATTTCCA GTTAAAATCT 
AATCCAGCCT CTTTTGAAGA AGCTCTGAGG GTTTTTGCTG ATCTTCCTAA ACTGCTGGGA 
GAAACCAAGG AGTGTGCAGT TCCACTGAGA GTGTGGCTTT ATCCACTGGA CAAACTGCAT 
TCACATTATG TGAAACTTCA ACAAGAAATC AGCATAGATC TAATCAGAAA TGTTGAATCA 
GTATTTGAGA GCTTAAGGAC CACTGAAATG AAATGCAGTG ATCTTCTGAA TGATACACCA 
TCTTTGGCTT TTGCAGGATT TCATGATAAA ATAATGATTA TGAAGCAAAA CTGCTGCAAC 
TATAAGCTGA GCTTCATGAA GAAACTTGGA TCGCTATTGC CAAAAATCCG TAGTGATACA 
GAAAATGAGA CAGCCCTGAT TGAGCTCCTC CATGATCATG AAAAATGTCC ATTCAGAAAA 
AGGGATCTTG AGAAATGGGT GAAAGAAAAA GAGAATGAAT CCGTCATTAT TAAAACTCTT 
CTCAGACAAT TGACTGATTC TGGAGCAACA GTGGAAAATA ATCTAGATGG AATTTTAATG 
GATCTTAATG TTGAAAATGT AGTAAGCTAC ACTTTCACAT CACTTGAGTG TCCAGATGTG 
CTCCTTACTA AACAAAAGGC TTTCCTGAAT CCATCAACAA TAGAAAACAA CAGTGAGGAT 
GCTTCTGTTT TAAGTAAAAA ATCTGGGTTC ACATCTGACA TCAAAACCAC AATGAGGAGC 
AACTTGATCA TCTTCAAAAA CTTGATGGAA TCAAAAATAT GTAAACCAGC CAAATATATT 
GTTGCATCGA AGGAAATACC AAATCCAGGT TCCTGCATTC TCATTTACGA AAATGGATCT 
GATGAAGCGA CATGCTTTAC TCCGCCAATA AAACCAGCTC ATCCAGTCAC TGAACAGATC 
AGTGGGGAAA GAGTGGTTTT AAAGGTGACA CCTGTGTGTC CTGCAACAGA AGAGCTCAGG 
TTACTGTACA AAACAAAAGA AGAGAAAGAC TGGAAATCTC AGTCTGTGAA AAAGAGTCAA 
GATACAGTTA TTCTGACTGA TCTCAGACCA GACACTGAGT ATGAGATGAA ATTTGCAGCC 
GTGGGGAAGC TGAACTACAC CATAGACAGT GATGTGATTC ACCTCAGAGT TATTGACAAA 
AACCTCACTG ATGCCACAGA GTCCATTTTA GAAAAGCTGA GCTTGATAGA AGACAAGTGC 
AGTAAACTGA TGGAGGGCAA GAGTGCAGTC ACATTTAGAG CAATTCGCAA CAAGTTTGAA 
GACATGAAGA GACACTGTCA GACTTACAAA CAAGACAGAA TTAAATCCAC AATCCAGTCA 
GTTCAAGCCT GTGAAAAAGA CATCAGTGCT CTGACAGACC TTCTACAAGC TCATCATGAG 
TCTCCATTTA ACATAAGTGA TATTGTGGAG TGGATCAGAG TGAAAGAAAC CGAATCAAAA 
GCAGTTGAGA AGATTCTTCA ACAGCTGCTG GAAACTGGAG CTGAAGTGAA CAATAGTTTA 
GATGAATATT TGTTGGATGT TAGTTTTGAG AATGTGGTGT GTTACACATT CACTTCTCTT 
GATCTGCCAG ACGACCTGCT TTCTGATCAA GAAGATTTCC TGAAACCTGA AATGATAAGG 
AGAAATTCTG ATAAGAAACC CAATGCTGTG TCTGAAACTT GGCTCACAGG AAGTACCAGA 
GAGAAATTGA AAAAACAATT AGAAATGTTT AAAGAGCTCA AATGTTCATA TGGCAGTCAC 
TCAACAAAGT TTTTGGTTAT ATCAGAAGAT CAGACAAACC ATCCAGGTTC CTGTATTCTC 
CTTTATGAAA ATGGATCTTA TAAAGCGAAA TGCTTTACTC CGCCAATAAA ACCAGCTGAG 
CCCATCATTG AACAGGTCAT CGGGGAAAGA GTGGTTTTGA AGGTGACATC TGTGTGTCCT 
GCAACAGAAG AGCTCAGGTT ACTGTACAAA ACAAAAGAAG AGAACGACTG GAAATCTCAG 
TCTGTGAAAA AGAGTCAAGA TACAGTTATT CTGACTGATC TCAGACCAGA CACTGAGTAT 
GAGATGAAAT TTGCAGCTGT GGGGAAGCTG AACTACACCA TAGACAGTGA TGTGATTCAC 
CTTAGAGTTA TTGACAAGAA ACTCACTGAT GCCACAGAGT CCATTTTAGA AAAGCTGAGC 
TTGATAGAAG ACAAGTGCAG TAAACTGATG GAGGACAAGA GTGCAGTCAC TTTTAGAGCA 
ATTCACAACA AGTTTGAAGA CATGAGAAGA CACTGTCAGA TCTACAAACA AGACTTAGAA 
ATTAAAATTA CATCCACAAT GCAGTCAGTT CAAACCTGTG AAAAAGACAT CAGTGCTCTG 
ACAGACCTTC TACAAGCTCA TCATGAGTCT CCATTTAATG AAAGTGATCT TATAGAGTGG 
ATCAGAGTGA AAGAAACCGA ATCAAATGCA GTTGAGAAGA TTCTTCAACA GCTGCTGGAC 
TCTGGAACTG AGGTGAACAA TAGTTTAGAT GAATATTTGT TTGATGTTAG TTTTGAGAAT 
GTGGTGTGTT ACACATTCAC TTCTCTTGAT CTGCCAGACA ACCTGCTTTC TAAACAAGGA 
GATTTCCTGA AACCTGAAAT AATGAGGAGA AATTCTAATA AGAACCCCAA TTCTGTGTCT 
GAAACTTGGC TCACAGGAAG TATCAGAGAG ACTATGAAGA AGCATTTGGA AATATTTAAT 
GAGCTCAAAT GTTCATATGG CAGTCACTCA TCAACATTTT TGGTTGTATA TAAAAGTCAC 
ACAAACCATC CAGGTTCCTG CATTCTTATT TATGAAAATG GATCTGATAA AGCGACATGC 
TTCACTCCTC CAATAAAACC AGCTAGTCCA GTCATTGAAC AGATTGGTGA GGATATAGTG 
GTTTTGAAGG CAACACCATT ATGTCCTGCA ACACAAGAGC TCAGGTTACT GTACAAAAAA 
AAAGAAGAGA ATGATTGGAA ATCTCAGTCT GTGAAAACGA GTGGAGATGA AGTTTTTCTG 
ACTGGTCTCA GACCTGACAC TGAGTATAAG ATGAAATATG TGGCCTTGGG GAAGCTGAAC 
TACACCACAG ACAGTGATGT CATCGGAGTT ACTACACGTG TGGTCAGTGA GAAACAGTGT 
GCCTTGCCTT ATTCATGCAA TACTAATTTC ACATATTTTA ACAGTGAATT ATTGAACAGT 
AACTCTATGC AGCTAAATGA GACTGGCAAA TATCTGCAGG ATAAATCTGA ACAAAAACTA 
TCGATGACAA AGGAGCCAAG GAACAACCAA CCAATGTCAT TTGATTCTTC AGAGGAGGTC 
AAAGTGTGCA CTGTTTTGGC TGGAAAGACC AATAACTGTC AAACACATTT TATTGCATCA 
CTGCGAGCCC GAATTGAGCA TCTGACGGAA GTTCGTTCAG TAGACGAGAG TGACATTGTT 
TTAATTTTCT GTCCAATTGT TTCTCGAGCT GGAACTGACA TTGATGCAGC CATTCACAGA 
TGCTATACAA ATGCCTCTAA GCTGACTGTT CTAGTGGTGC TTCACCACAC GTTTGACCCA 
GAGAAAACTG TATCAGACAG CAGCAAAATT GTCAACAGGG AGAATCTGCT CACAGTGGAC 
TGTCTGTTCT ATGAGGATGC AGGATTACTG CAGTGTCAGA GGAATTCAGA TGCATATGAC 
AAATCTGTCA ACTGGTTAAT AGCGCAGGGC AAAAAAACAG GTCTTAAAAT CTACCCAAAG 
AAATCACCTG ATAGTCAATC CAATGGTCGG AAATTCTGGA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299675.1
CDS	299..4723
Translation	MDLGVGVNIVETAALGRPFRLGMLYDCRKDAFVPGITLWDEEQLQQSIKIHPQIKTDFCVTNSDFIEAKCQLLNINDCLKLSFLSGLINVSGAAKYLSDTKKSFKQQRLTLHYHSTTKREELTVNHLPSENITHYEVFENDAATHVVTAVLYGANACFVFDREASLVEDQSTVDRDVKAALEKLKGISESTQIDLSLDDNQKTVFEKFSCTFYGDFQLKSNPASFEEALRVFADLPKLLGETKECAVPLRVWLYPLDKLHSHYVKLQQEISIDLIRNVESVFESLRTTEMKCSDLLNDTPSLAFAGFHDKIMIMKQNCCNYKLSFMKKLGSLLPKIRSDTENETALIELLHDHEKCPFRKRDLEKWVKEKENESVIIKTLLRQLTDSGATVENNLDGILMDLNVENVVSYTFTSLECPDVLLTKQKAFLNPSTIENNSEDASVLSKKSGFTSDIKTTMRSNLIIFKNLMESKICKPAKYIVASKEIPNPGSCILIYENGSDEATCFTPPIKPAHPVTEQISGERVVLKVTPVCPATEELRLLYKTKEEKDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKNLTDATESILEKLSLIEDKCSKLMEGKSAVTFRAIRNKFEDMKRHCQTYKQDRIKSTIQSVQACEKDISALTDLLQAHHESPFNISDIVEWIRVKETESKAVEKILQQLLETGAEVNNSLDEYLLDVSFENVVCYTFTSLDLPDDLLSDQEDFLKPEMIRRNSDKKPNAVSETWLTGSTREKLKKQLEMFKELKCSYGSHSTKFLVISEDQTNHPGSCILLYENGSYKAKCFTPPIKPAEPIIEQVIGERVVLKVTSVCPATEELRLLYKTKEENDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKKLTDATESILEKLSLIEDKCSKLMEDKSAVTFRAIHNKFEDMRRHCQIYKQDLEIKITSTMQSVQTCEKDISALTDLLQAHHESPFNESDLIEWIRVKETESNAVEKILQQLLDSGTEVNNSLDEYLFDVSFENVVCYTFTSLDLPDNLLSKQGDFLKPEIMRRNSNKNPNSVSETWLTGSIRETMKKHLEIFNELKCSYGSHSSTFLVVYKSHTNHPGSCILIYENGSDKATCFTPPIKPASPVIEQIGEDIVVLKATPLCPATQELRLLYKKKEENDWKSQSVKTSGDEVFLTGLRPDTEYKMKYVALGKLNYTTDSDVIGVTTRVVSEKQCALPYSCNTNFTYFNSELLNSNSMQLNETGKYLQDKSEQKLSMTKEPRNNQPMSFDSSEEVKVCTVLAGKTNNCQTHFIASLRARIEHLTEVRSVDESDIVLIFCPIVSRAGTDIDAAIHRCYTNASKLTVLVVLHHTFDPEKTVSDSSKIVNRENLLTVDCLFYEDAGLLQCQRNSDAYDKSVNWLIAQGKKTGLKIYPKKSPDSQSNGRKFWR

Target ORF information:

RefSeq Version	XM_009301400.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301400.3

ORF Insert Sequence:

ATGGACTTGG GAGTGGGAGT TAACATTGTT GAAACAGCAG CTCTGGGGAG ACCCTTCAGG 
CTGGGGATGC TGTACGACTG CAGAAAAGAT GCATTTGTAC CAGGAATCAC ACTGTGGGAT 
GAAGAGCAAC TGCAGCAGAG CATAAAAATC CATCCTCAAA TTAAAACAGA TTTCTGTGTT 
ACAAATTCAG ACTTTATTGA GGCAAAATGC CAATTACTGA ACATTAATGA TTGTCTAAAA 
CTGAGTTTTT TAAGTGGACT CATCAATGTT AGTGGAGCAG CCAAGTATCT CAGTGACACC 
AAGAAATCCT TCAAACAGCA GAGACTGACC CTACATTATC ATTCAACCAC CAAACGTGAA 
GAACTGACTG TGAACCACTT GCCATCTGAA AATATAACCC ACTATGAAGT GTTTGAGAAT 
GATGCAGCGA CACATGTGGT GACAGCTGTG CTGTATGGGG CCAATGCTTG CTTTGTTTTT 
GACAGAGAAG CTTCATTAGT TGAAGACCAA AGCACTGTAG ACAGAGACGT AAAGGCTGCC 
TTAGAAAAAC TAAAAGGGAT TTCAGAAAGT ACACAGATTG ATCTGAGCTT GGATGACAAT 
CAAAAGACTG TTTTTGAGAA ATTCAGCTGC ACATTTTATG GTGATTTCCA GTTAAAATCT 
AATCCAGCCT CTTTTGAAGA AGCTCTGAGG GTTTTTGCTG ATCTTCCTAA ACTGCTGGGA 
GAAACCAAGG AGTGTGCAGT TCCACTGAGA GTGTGGCTTT ATCCACTGGA CAAACTGCAT 
TCACATTATG TGAAACTTCA ACAAGAAATC AGCATAGATC TAATCAGAAA TGTTGAATCA 
GTATTTGAGA GCTTAAGGAC CACTGAAATG AAATGCAGTG ATCTTCTGAA TGATACACCA 
TCTTTGGCTT TTGCAGGATT TCATGATAAA ATAATGATTA TGAAGCAAAA CTGCTGCAAC 
TATAAGCTGA GCTTCATGAA GAAACTTGGA TCGCTATTGC CAAAAATCCG TAGTGATACA 
GAAAATGAGA CAGCCCTGAT TGAGCTCCTC CATGATCATG AAAAATGTCC ATTCAGAAAA 
AGGGATCTTG AGAAATGGGT GAAAGAAAAA GAGAATGAAT CCGTCATTAT TAAAACTCTT 
CTCAGACAAT TGACTGATTC TGGAGCAACA GTGGAAAATA ATCTAGATGG AATTTTAATG 
GATCTTAATG TTGAAAATGT AGTAAGCTAC ACTTTCACAT CACTTGAGTG TCCAGATGTG 
CTCCTTACTA AACAAAAGGC TTTCCTGAAT CCATCAACAA TAGAAAACAA CAGTGAGGAT 
GCTTCTGTTT TAAGTAAAAA ATCTGGGTTC ACATCTGACA TCAAAACCAC AATGAGGAGC 
AACTTGATCA TCTTCAAAAA CTTGATGGAA TCAAAAATAT GTAAACCAGC CAAATATATT 
GTTGCATCGA AGGAAATACC AAATCCAGGT TCCTGCATTC TCATTTACGA AAATGGATCT 
GATGAAGCGA CATGCTTTAC TCCGCCAATA AAACCAGCTC ATCCAGTCAC TGAACAGATC 
AGTGGGGAAA GAGTGGTTTT AAAGGTGACA CCTGTGTGTC CTGCAACAGA AGAGCTCAGG 
TTACTGTACA AAACAAAAGA AGAGAAAGAC TGGAAATCTC AGTCTGTGAA AAAGAGTCAA 
GATACAGTTA TTCTGACTGA TCTCAGACCA GACACTGAGT ATGAGATGAA ATTTGCAGCC 
GTGGGGAAGC TGAACTACAC CATAGACAGT GATGTGATTC ACCTCAGAGT TATTGACAAA 
AACCTCACTG ATGCCACAGA GTCCATTTTA GAAAAGCTGA GCTTGATAGA AGACAAGTGC 
AGTAAACTGA TGGAGGGCAA GAGTGCAGTC ACATTTAGAG CAATTCGCAA CAAGTTTGAA 
GACATGAAGA GACACTGTCA GACTTACAAA CAAGACAGAA TTAAATCCAC AATCCAGTCA 
GTTCAAGCCT GTGAAAAAGA CATCAGTGCT CTGACAGACC TTCTACAAGC TCATCATGAG 
TCTCCATTTA ACATAAGTGA TATTGTGGAG TGGATCAGAG TGAAAGAAAC CGAATCAAAA 
GCAGTTGAGA AGATTCTTCA ACAGCTGCTG GAAACTGGAG CTGAAGTGAA CAATAGTTTA 
GATGAATATT TGTTGGATGT TAGTTTTGAG AATGTGGTGT GTTACACATT CACTTCTCTT 
GATCTGCCAG ACGACCTGCT TTCTGATCAA GAAGATTTCC TGAAACCTGA AATGATAAGG 
AGAAATTCTG ATAAGAAACC CAATGCTGTG TCTGAAACTT GGCTCACAGG AAGTACCAGA 
GAGAAATTGA AAAAACAATT AGAAATGTTT AAAGAGCTCA AATGTTCATA TGGCAGTCAC 
TCAACAAAGT TTTTGGTTAT ATCAGAAGAT CAGACAAACC ATCCAGGTTC CTGTATTCTC 
CTTTATGAAA ATGGATCTTA TAAAGCGAAA TGCTTTACTC CGCCAATAAA ACCAGCTGAG 
CCCATCATTG AACAGGTCAT CGGGGAAAGA GTGGTTTTGA AGGTGACATC TGTGTGTCCT 
GCAACAGAAG AGCTCAGGTT ACTGTACAAA ACAAAAGAAG AGAACGACTG GAAATCTCAG 
TCTGTGAAAA AGAGTCAAGA TACAGTTATT CTGACTGATC TCAGACCAGA CACTGAGTAT 
GAGATGAAAT TTGCAGCTGT GGGGAAGCTG AACTACACCA TAGACAGTGA TGTGATTCAC 
CTTAGAGTTA TTGACAAGAA ACTCACTGAT GCCACAGAGT CCATTTTAGA AAAGCTGAGC 
TTGATAGAAG ACAAGTGCAG TAAACTGATG GAGGACAAGA GTGCAGTCAC TTTTAGAGCA 
ATTCACAACA AGTTTGAAGA CATGAGAAGA CACTGTCAGA TCTACAAACA AGACTTAGAA 
ATTAAAATTA CATCCACAAT GCAGTCAGTT CAAACCTGTG AAAAAGACAT CAGTGCTCTG 
ACAGACCTTC TACAAGCTCA TCATGAGTCT CCATTTAATG AAAGTGATCT TATAGAGTGG 
ATCAGAGTGA AAGAAACCGA ATCAAATGCA GTTGAGAAGA TTCTTCAACA GCTGCTGGAC 
TCTGGAACTG AGGTGAACAA TAGTTTAGAT GAATATTTGT TTGATGTTAG TTTTGAGAAT 
GTGGTGTGTT ACACATTCAC TTCTCTTGAT CTGCCAGACA ACCTGCTTTC TAAACAAGGA 
GATTTCCTGA AACCTGAAAT AATGAGGAGA AATTCTAATA AGAACCCCAA TTCTGTGTCT 
GAAACTTGGC TCACAGGAAG TATCAGAGAG ACTATGAAGA AGCATTTGGA AATATTTAAT 
GAGCTCAAAT GTTCATATGG CAGTCACTCA TCAACATTTT TGGTTGTATA TAAAAGTCAC 
ACAAACCATC CAGGTTCCTG CATTCTTATT TATGAAAATG GATCTGATAA AGCGACATGC 
TTCACTCCTC CAATAAAACC AGCTAGTCCA GTCATTGAAC AGATTGGTGA GGATATAGTG 
GTTTTGAAGG CAACACCATT ATGTCCTGCA ACACAAGAGC TCAGGTTACT GTACAAAAAA 
AAAGAAGAGA ATGATTGGAA ATCTCAGTCT GTGAAAACGA GTGGAGATGA AGTTTTTCTG 
ACTGGTCTCA GACCTGACAC TGAGTATAAG ATGAAATATG TGGCCTTGGG GAAGCTGAAC 
TACACCACAG ACAGTGATGT CATCGGAGTT ACTACACGTG TGGTCAGTGA GAAACAGTGT 
GCCTTGCCTT ATTCATGCAA TACTAATTTC ACATATTTTA ACAGTGAATT ATTGAACAGT 
AACTCTATGC AGCTAAATGA GACTGGCAAA TATCTGCAGG ATAAATCTGA ACAAAAACTA 
TCGATGACAA AGGAGCCAAG GAACAACCAA CCAATGTCAT TTGATTCTTC AGAGGAGGTC 
AAAGTGTGCA CTGTTTTGGC TGGAAAGACC AATAACTGTC AAACACATTT TATTGCATCA 
CTGCGAGCCC GAATTGAGCA TCTGACGGAA GTTCGTTCAG TAGACGAGAG TGACATTGTT 
TTAATTTTCT GTCCAATTGT TTCTCGAGCT GGAACTGACA TTGATGCAGC CATTCACAGA 
TGCTATACAA ATGCCTCTAA GCTGACTGTT CTAGTGGTGC TTCACCACAC GTTTGACCCA 
GAGAAAACTG TATCAGACAG CAGCAAAATT GTCAACAGGG AGAATCTGCT CACAGTGGAC 
TGTCTGTTCT ATGAGGATGC AGGATTACTG CAGTGTCAGA GGAATTCAGA TGCATATGAC 
AAATCTGTCA ACTGGTTAAT AGCGCAGGGC AAAAAAACAG GTCTTAAAAT CTACCCAAAG 
AAATCACCTG ATAGTCAATC CAATGGTCGG AAATTCTGGA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100148510 ( XM_009301401.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299676.1 Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X3, mRNA.

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CloneID	ODa10353
Clone ID Related Accession (Same CDS sequence)	XM_021476449.1 , XM_021476450.1 , XM_009301400.2 , XM_009301401.2 , XM_009301400.3 , XM_009301401.3
Accession Version	XM_009301401.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC100148510 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301401.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTGG GAGTGGGAGT TAACATTGTT GAAACAGCAG CTCTGGGGAG ACCCTTCAGG 
CTGGGGATGC TGTACGACTG CAGAAAAGAT GCATTTGTAC CAGGAATCAC ACTGTGGGAT 
GAAGAGCAAC TGCAGCAGAG CATAAAAATC CATCCTCAAA TTAAAACAGA TTTCTGTGTT 
ACAAATTCAG ACTTTATTGA GGCAAAATGC CAATTACTGA ACATTAATGA TTGTCTAAAA 
CTGAGTTTTT TAAGTGGACT CATCAATGTT AGTGGAGCAG CCAAGTATCT CAGTGACACC 
AAGAAATCCT TCAAACAGCA GAGACTGACC CTACATTATC ATTCAACCAC CAAACGTGAA 
GAACTGACTG TGAACCACTT GCCATCTGAA AATATAACCC ACTATGAAGT GTTTGAGAAT 
GATGCAGCGA CACATGTGGT GACAGCTGTG CTGTATGGGG CCAATGCTTG CTTTGTTTTT 
GACAGAGAAG CTTCATTAGT TGAAGACCAA AGCACTGTAG ACAGAGACGT AAAGGCTGCC 
TTAGAAAAAC TAAAAGGGAT TTCAGAAAGT ACACAGATTG ATCTGAGCTT GGATGACAAT 
CAAAAGACTG TTTTTGAGAA ATTCAGCTGC ACATTTTATG GTGATTTCCA GTTAAAATCT 
AATCCAGCCT CTTTTGAAGA AGCTCTGAGG GTTTTTGCTG ATCTTCCTAA ACTGCTGGGA 
GAAACCAAGG AGTGTGCAGT TCCACTGAGA GTGTGGCTTT ATCCACTGGA CAAACTGCAT 
TCACATTATG TGAAACTTCA ACAAGAAATC AGCATAGATC TAATCAGAAA TGTTGAATCA 
GTATTTGAGA GCTTAAGGAC CACTGAAATG AAATGCAGTG ATCTTCTGAA TGATACACCA 
TCTTTGGCTT TTGCAGGATT TCATGATAAA ATAATGATTA TGAAGCAAAA CTGCTGCAAC 
TATAAGCTGA GCTTCATGAA GAAACTTGGA TCGCTATTGC CAAAAATCCG TAGTGATACA 
GAAAATGAGA CAGCCCTGAT TGAGCTCCTC CATGATCATG AAAAATGTCC ATTCAGAAAA 
AGGGATCTTG AGAAATGGGT GAAAGAAAAA GAGAATGAAT CCGTCATTAT TAAAACTCTT 
CTCAGACAAT TGACTGATTC TGGAGCAACA GTGGAAAATA ATCTAGATGG AATTTTAATG 
GATCTTAATG TTGAAAATGT AGTAAGCTAC ACTTTCACAT CACTTGAGTG TCCAGATGTG 
CTCCTTACTA AACAAAAGGC TTTCCTGAAT CCATCAACAA TAGAAAACAA CAGTGAGGAT 
GCTTCTGTTT TAAGTAAAAA ATCTGGGTTC ACATCTGACA TCAAAACCAC AATGAGGAGC 
AACTTGATCA TCTTCAAAAA CTTGATGGAA TCAAAAATAT GTAAACCAGC CAAATATATT 
GTTGCATCGA AGGAAATACC AAATCCAGGT TCCTGCATTC TCATTTACGA AAATGGATCT 
GATGAAGCGA CATGCTTTAC TCCGCCAATA AAACCAGCTC ATCCAGTCAC TGAACAGATC 
AGTGGGGAAA GAGTGGTTTT AAAGGTGACA CCTGTGTGTC CTGCAACAGA AGAGCTCAGG 
TTACTGTACA AAACAAAAGA AGAGAAAGAC TGGAAATCTC AGTCTGTGAA AAAGAGTCAA 
GATACAGTTA TTCTGACTGA TCTCAGACCA GACACTGAGT ATGAGATGAA ATTTGCAGCC 
GTGGGGAAGC TGAACTACAC CATAGACAGT GATGTGATTC ACCTCAGAGT TATTGACAAA 
AACCTCACTG ATGCCACAGA GTCCATTTTA GAAAAGCTGA GCTTGATAGA AGACAAGTGC 
AGTAAACTGA TGGAGGGCAA GAGTGCAGTC ACATTTAGAG CAATTCGCAA CAAGTTTGAA 
GACATGAAGA GACACTGTCA GACTTACAAA CAAGACAGAA TTAAATCCAC AATCCAGTCA 
GTTCAAGCCT GTGAAAAAGA CATCAGTGCT CTGACAGACC TTCTACAAGC TCATCATGAG 
TCTCCATTTA ACATAAGTGA TATTGTGGAG TGGATCAGAG TGAAAGAAAC CGAATCAAAA 
GCAGTTGAGA AGATTCTTCA ACAGCTGCTG GAAACTGGAG CTGAAGTGAA CAATAGTTTA 
GATGAATATT TGTTGGATGT TAGTTTTGAG AATGTGGTGT GTTACACATT CACTTCTCTT 
GATCTGCCAG ACGACCTGCT TTCTGATCAA GAAGATTTCC TGAAACCTGA AATGATAAGG 
AGAAATTCTG ATAAGAAACC CAATGCTGTG TCTGAAACTT GGCTCACAGG AAGTACCAGA 
GAGAAATTGA AAAAACAATT AGAAATGTTT AAAGAGCTCA AATGTTCATA TGGCAGTCAC 
TCAACAAAGT TTTTGGTTAT ATCAGAAGAT CAGACAAACC ATCCAGGTTC CTGTATTCTC 
CTTTATGAAA ATGGATCTTA TAAAGCGAAA TGCTTTACTC CGCCAATAAA ACCAGCTGAG 
CCCATCATTG AACAGGTCAT CGGGGAAAGA GTGGTTTTGA AGGTGACATC TGTGTGTCCT 
GCAACAGAAG AGCTCAGGTT ACTGTACAAA ACAAAAGAAG AGAACGACTG GAAATCTCAG 
TCTGTGAAAA AGAGTCAAGA TACAGTTATT CTGACTGATC TCAGACCAGA CACTGAGTAT 
GAGATGAAAT TTGCAGCTGT GGGGAAGCTG AACTACACCA TAGACAGTGA TGTGATTCAC 
CTTAGAGTTA TTGACAAGAA ACTCACTGAT GCCACAGAGT CCATTTTAGA AAAGCTGAGC 
TTGATAGAAG ACAAGTGCAG TAAACTGATG GAGGACAAGA GTGCAGTCAC TTTTAGAGCA 
ATTCACAACA AGTTTGAAGA CATGAGAAGA CACTGTCAGA TCTACAAACA AGACTTAGAA 
ATTAAAATTA CATCCACAAT GCAGTCAGTT CAAACCTGTG AAAAAGACAT CAGTGCTCTG 
ACAGACCTTC TACAAGCTCA TCATGAGTCT CCATTTAATG AAAGTGATCT TATAGAGTGG 
ATCAGAGTGA AAGAAACCGA ATCAAATGCA GTTGAGAAGA TTCTTCAACA GCTGCTGGAC 
TCTGGAACTG AGGTGAACAA TAGTTTAGAT GAATATTTGT TTGATGTTAG TTTTGAGAAT 
GTGGTGTGTT ACACATTCAC TTCTCTTGAT CTGCCAGACA ACCTGCTTTC TAAACAAGGA 
GATTTCCTGA AACCTGAAAT AATGAGGAGA AATTCTAATA AGAACCCCAA TTCTGTGTCT 
GAAACTTGGC TCACAGGAAG TATCAGAGAG ACTATGAAGA AGCATTTGGA AATATTTAAT 
GAGCTCAAAT GTTCATATGG CAGTCACTCA TCAACATTTT TGGTTGTATA TAAAAGTCAC 
ACAAACCATC CAGGTTCCTG CATTCTTATT TATGAAAATG GATCTGATAA AGCGACATGC 
TTCACTCCTC CAATAAAACC AGCTAGTCCA GTCATTGAAC AGATTGGTGA GGATATAGTG 
GTTTTGAAGG CAACACCATT ATGTCCTGCA ACACAAGAGC TCAGGTTACT GTACAAAAAA 
AAAGAAGAGA ATGATTGGAA ATCTCAGTCT GTGAAAACGA GTGGAGATGA AGTTTTTCTG 
ACTGGTCTCA GACCTGACAC TGAGTATAAG ATGAAATATG TGGCCTTGGG GAAGCTGAAC 
TACACCACAG ACAGTGATGT CATCGGAGTT ACTACACGTG TGGTCAGTGA GAAACAGTGT 
GCCTTGCCTT ATTCATGCAA TACTAATTTC ACATATTTTA ACAGTGAATT ATTGAACAGT 
AACTCTATGC AGCTAAATGA GACTGGCAAA TATCTGCAGG ATAAATCTGA ACAAAAACTA 
TCGATGACAA AGGAGCCAAG GAACAACCAA CCAATGTCAT TTGATTCTTC AGAGGAGGTC 
AAAGTGTGCA CTGTTTTGGC TGGAAAGACC AATAACTGTC AAACACATTT TATTGCATCA 
CTGCGAGCCC GAATTGAGCA TCTGACGGAA GTTCGTTCAG TAGACGAGAG TGACATTGTT 
TTAATTTTCT GTCCAATTGT TTCTCGAGCT GGAACTGACA TTGATGCAGC CATTCACAGA 
TGCTATACAA ATGCCTCTAA GCTGACTGTT CTAGTGGTGC TTCACCACAC GTTTGACCCA 
GAGAAAACTG TATCAGACAG CAGCAAAATT GTCAACAGGG AGAATCTGCT CACAGTGGAC 
TGTCTGTTCT ATGAGGATGC AGGATTACTG CAGTGTCAGA GGAATTCAGA TGCATATGAC 
AAATCTGTCA ACTGGTTAAT AGCGCAGGGC AAAAAAACAG GTCTTAAAAT CTACCCAAAG 
AAATCACCTG ATAGTCAATC CAATGGTCGG AAATTCTGGA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299676.1
CDS	621..5045
Translation	MDLGVGVNIVETAALGRPFRLGMLYDCRKDAFVPGITLWDEEQLQQSIKIHPQIKTDFCVTNSDFIEAKCQLLNINDCLKLSFLSGLINVSGAAKYLSDTKKSFKQQRLTLHYHSTTKREELTVNHLPSENITHYEVFENDAATHVVTAVLYGANACFVFDREASLVEDQSTVDRDVKAALEKLKGISESTQIDLSLDDNQKTVFEKFSCTFYGDFQLKSNPASFEEALRVFADLPKLLGETKECAVPLRVWLYPLDKLHSHYVKLQQEISIDLIRNVESVFESLRTTEMKCSDLLNDTPSLAFAGFHDKIMIMKQNCCNYKLSFMKKLGSLLPKIRSDTENETALIELLHDHEKCPFRKRDLEKWVKEKENESVIIKTLLRQLTDSGATVENNLDGILMDLNVENVVSYTFTSLECPDVLLTKQKAFLNPSTIENNSEDASVLSKKSGFTSDIKTTMRSNLIIFKNLMESKICKPAKYIVASKEIPNPGSCILIYENGSDEATCFTPPIKPAHPVTEQISGERVVLKVTPVCPATEELRLLYKTKEEKDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKNLTDATESILEKLSLIEDKCSKLMEGKSAVTFRAIRNKFEDMKRHCQTYKQDRIKSTIQSVQACEKDISALTDLLQAHHESPFNISDIVEWIRVKETESKAVEKILQQLLETGAEVNNSLDEYLLDVSFENVVCYTFTSLDLPDDLLSDQEDFLKPEMIRRNSDKKPNAVSETWLTGSTREKLKKQLEMFKELKCSYGSHSTKFLVISEDQTNHPGSCILLYENGSYKAKCFTPPIKPAEPIIEQVIGERVVLKVTSVCPATEELRLLYKTKEENDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKKLTDATESILEKLSLIEDKCSKLMEDKSAVTFRAIHNKFEDMRRHCQIYKQDLEIKITSTMQSVQTCEKDISALTDLLQAHHESPFNESDLIEWIRVKETESNAVEKILQQLLDSGTEVNNSLDEYLFDVSFENVVCYTFTSLDLPDNLLSKQGDFLKPEIMRRNSNKNPNSVSETWLTGSIRETMKKHLEIFNELKCSYGSHSSTFLVVYKSHTNHPGSCILIYENGSDKATCFTPPIKPASPVIEQIGEDIVVLKATPLCPATQELRLLYKKKEENDWKSQSVKTSGDEVFLTGLRPDTEYKMKYVALGKLNYTTDSDVIGVTTRVVSEKQCALPYSCNTNFTYFNSELLNSNSMQLNETGKYLQDKSEQKLSMTKEPRNNQPMSFDSSEEVKVCTVLAGKTNNCQTHFIASLRARIEHLTEVRSVDESDIVLIFCPIVSRAGTDIDAAIHRCYTNASKLTVLVVLHHTFDPEKTVSDSSKIVNRENLLTVDCLFYEDAGLLQCQRNSDAYDKSVNWLIAQGKKTGLKIYPKKSPDSQSNGRKFWR

Target ORF information:

RefSeq Version	XM_009301401.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301401.3

ORF Insert Sequence:

ATGGACTTGG GAGTGGGAGT TAACATTGTT GAAACAGCAG CTCTGGGGAG ACCCTTCAGG 
CTGGGGATGC TGTACGACTG CAGAAAAGAT GCATTTGTAC CAGGAATCAC ACTGTGGGAT 
GAAGAGCAAC TGCAGCAGAG CATAAAAATC CATCCTCAAA TTAAAACAGA TTTCTGTGTT 
ACAAATTCAG ACTTTATTGA GGCAAAATGC CAATTACTGA ACATTAATGA TTGTCTAAAA 
CTGAGTTTTT TAAGTGGACT CATCAATGTT AGTGGAGCAG CCAAGTATCT CAGTGACACC 
AAGAAATCCT TCAAACAGCA GAGACTGACC CTACATTATC ATTCAACCAC CAAACGTGAA 
GAACTGACTG TGAACCACTT GCCATCTGAA AATATAACCC ACTATGAAGT GTTTGAGAAT 
GATGCAGCGA CACATGTGGT GACAGCTGTG CTGTATGGGG CCAATGCTTG CTTTGTTTTT 
GACAGAGAAG CTTCATTAGT TGAAGACCAA AGCACTGTAG ACAGAGACGT AAAGGCTGCC 
TTAGAAAAAC TAAAAGGGAT TTCAGAAAGT ACACAGATTG ATCTGAGCTT GGATGACAAT 
CAAAAGACTG TTTTTGAGAA ATTCAGCTGC ACATTTTATG GTGATTTCCA GTTAAAATCT 
AATCCAGCCT CTTTTGAAGA AGCTCTGAGG GTTTTTGCTG ATCTTCCTAA ACTGCTGGGA 
GAAACCAAGG AGTGTGCAGT TCCACTGAGA GTGTGGCTTT ATCCACTGGA CAAACTGCAT 
TCACATTATG TGAAACTTCA ACAAGAAATC AGCATAGATC TAATCAGAAA TGTTGAATCA 
GTATTTGAGA GCTTAAGGAC CACTGAAATG AAATGCAGTG ATCTTCTGAA TGATACACCA 
TCTTTGGCTT TTGCAGGATT TCATGATAAA ATAATGATTA TGAAGCAAAA CTGCTGCAAC 
TATAAGCTGA GCTTCATGAA GAAACTTGGA TCGCTATTGC CAAAAATCCG TAGTGATACA 
GAAAATGAGA CAGCCCTGAT TGAGCTCCTC CATGATCATG AAAAATGTCC ATTCAGAAAA 
AGGGATCTTG AGAAATGGGT GAAAGAAAAA GAGAATGAAT CCGTCATTAT TAAAACTCTT 
CTCAGACAAT TGACTGATTC TGGAGCAACA GTGGAAAATA ATCTAGATGG AATTTTAATG 
GATCTTAATG TTGAAAATGT AGTAAGCTAC ACTTTCACAT CACTTGAGTG TCCAGATGTG 
CTCCTTACTA AACAAAAGGC TTTCCTGAAT CCATCAACAA TAGAAAACAA CAGTGAGGAT 
GCTTCTGTTT TAAGTAAAAA ATCTGGGTTC ACATCTGACA TCAAAACCAC AATGAGGAGC 
AACTTGATCA TCTTCAAAAA CTTGATGGAA TCAAAAATAT GTAAACCAGC CAAATATATT 
GTTGCATCGA AGGAAATACC AAATCCAGGT TCCTGCATTC TCATTTACGA AAATGGATCT 
GATGAAGCGA CATGCTTTAC TCCGCCAATA AAACCAGCTC ATCCAGTCAC TGAACAGATC 
AGTGGGGAAA GAGTGGTTTT AAAGGTGACA CCTGTGTGTC CTGCAACAGA AGAGCTCAGG 
TTACTGTACA AAACAAAAGA AGAGAAAGAC TGGAAATCTC AGTCTGTGAA AAAGAGTCAA 
GATACAGTTA TTCTGACTGA TCTCAGACCA GACACTGAGT ATGAGATGAA ATTTGCAGCC 
GTGGGGAAGC TGAACTACAC CATAGACAGT GATGTGATTC ACCTCAGAGT TATTGACAAA 
AACCTCACTG ATGCCACAGA GTCCATTTTA GAAAAGCTGA GCTTGATAGA AGACAAGTGC 
AGTAAACTGA TGGAGGGCAA GAGTGCAGTC ACATTTAGAG CAATTCGCAA CAAGTTTGAA 
GACATGAAGA GACACTGTCA GACTTACAAA CAAGACAGAA TTAAATCCAC AATCCAGTCA 
GTTCAAGCCT GTGAAAAAGA CATCAGTGCT CTGACAGACC TTCTACAAGC TCATCATGAG 
TCTCCATTTA ACATAAGTGA TATTGTGGAG TGGATCAGAG TGAAAGAAAC CGAATCAAAA 
GCAGTTGAGA AGATTCTTCA ACAGCTGCTG GAAACTGGAG CTGAAGTGAA CAATAGTTTA 
GATGAATATT TGTTGGATGT TAGTTTTGAG AATGTGGTGT GTTACACATT CACTTCTCTT 
GATCTGCCAG ACGACCTGCT TTCTGATCAA GAAGATTTCC TGAAACCTGA AATGATAAGG 
AGAAATTCTG ATAAGAAACC CAATGCTGTG TCTGAAACTT GGCTCACAGG AAGTACCAGA 
GAGAAATTGA AAAAACAATT AGAAATGTTT AAAGAGCTCA AATGTTCATA TGGCAGTCAC 
TCAACAAAGT TTTTGGTTAT ATCAGAAGAT CAGACAAACC ATCCAGGTTC CTGTATTCTC 
CTTTATGAAA ATGGATCTTA TAAAGCGAAA TGCTTTACTC CGCCAATAAA ACCAGCTGAG 
CCCATCATTG AACAGGTCAT CGGGGAAAGA GTGGTTTTGA AGGTGACATC TGTGTGTCCT 
GCAACAGAAG AGCTCAGGTT ACTGTACAAA ACAAAAGAAG AGAACGACTG GAAATCTCAG 
TCTGTGAAAA AGAGTCAAGA TACAGTTATT CTGACTGATC TCAGACCAGA CACTGAGTAT 
GAGATGAAAT TTGCAGCTGT GGGGAAGCTG AACTACACCA TAGACAGTGA TGTGATTCAC 
CTTAGAGTTA TTGACAAGAA ACTCACTGAT GCCACAGAGT CCATTTTAGA AAAGCTGAGC 
TTGATAGAAG ACAAGTGCAG TAAACTGATG GAGGACAAGA GTGCAGTCAC TTTTAGAGCA 
ATTCACAACA AGTTTGAAGA CATGAGAAGA CACTGTCAGA TCTACAAACA AGACTTAGAA 
ATTAAAATTA CATCCACAAT GCAGTCAGTT CAAACCTGTG AAAAAGACAT CAGTGCTCTG 
ACAGACCTTC TACAAGCTCA TCATGAGTCT CCATTTAATG AAAGTGATCT TATAGAGTGG 
ATCAGAGTGA AAGAAACCGA ATCAAATGCA GTTGAGAAGA TTCTTCAACA GCTGCTGGAC 
TCTGGAACTG AGGTGAACAA TAGTTTAGAT GAATATTTGT TTGATGTTAG TTTTGAGAAT 
GTGGTGTGTT ACACATTCAC TTCTCTTGAT CTGCCAGACA ACCTGCTTTC TAAACAAGGA 
GATTTCCTGA AACCTGAAAT AATGAGGAGA AATTCTAATA AGAACCCCAA TTCTGTGTCT 
GAAACTTGGC TCACAGGAAG TATCAGAGAG ACTATGAAGA AGCATTTGGA AATATTTAAT 
GAGCTCAAAT GTTCATATGG CAGTCACTCA TCAACATTTT TGGTTGTATA TAAAAGTCAC 
ACAAACCATC CAGGTTCCTG CATTCTTATT TATGAAAATG GATCTGATAA AGCGACATGC 
TTCACTCCTC CAATAAAACC AGCTAGTCCA GTCATTGAAC AGATTGGTGA GGATATAGTG 
GTTTTGAAGG CAACACCATT ATGTCCTGCA ACACAAGAGC TCAGGTTACT GTACAAAAAA 
AAAGAAGAGA ATGATTGGAA ATCTCAGTCT GTGAAAACGA GTGGAGATGA AGTTTTTCTG 
ACTGGTCTCA GACCTGACAC TGAGTATAAG ATGAAATATG TGGCCTTGGG GAAGCTGAAC 
TACACCACAG ACAGTGATGT CATCGGAGTT ACTACACGTG TGGTCAGTGA GAAACAGTGT 
GCCTTGCCTT ATTCATGCAA TACTAATTTC ACATATTTTA ACAGTGAATT ATTGAACAGT 
AACTCTATGC AGCTAAATGA GACTGGCAAA TATCTGCAGG ATAAATCTGA ACAAAAACTA 
TCGATGACAA AGGAGCCAAG GAACAACCAA CCAATGTCAT TTGATTCTTC AGAGGAGGTC 
AAAGTGTGCA CTGTTTTGGC TGGAAAGACC AATAACTGTC AAACACATTT TATTGCATCA 
CTGCGAGCCC GAATTGAGCA TCTGACGGAA GTTCGTTCAG TAGACGAGAG TGACATTGTT 
TTAATTTTCT GTCCAATTGT TTCTCGAGCT GGAACTGACA TTGATGCAGC CATTCACAGA 
TGCTATACAA ATGCCTCTAA GCTGACTGTT CTAGTGGTGC TTCACCACAC GTTTGACCCA 
GAGAAAACTG TATCAGACAG CAGCAAAATT GTCAACAGGG AGAATCTGCT CACAGTGGAC 
TGTCTGTTCT ATGAGGATGC AGGATTACTG CAGTGTCAGA GGAATTCAGA TGCATATGAC 
AAATCTGTCA ACTGGTTAAT AGCGCAGGGC AAAAAAACAG GTCTTAAAAT CTACCCAAAG 
AAATCACCTG ATAGTCAATC CAATGGTCGG AAATTCTGGA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100148510 ( XM_021476451.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332126.1 Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X4, mRNA.

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CloneID	ODa142778
Clone ID Related Accession (Same CDS sequence)	XM_021476451.1 , XM_021471583.1
Accession Version	XM_021476451.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC100148510 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAGTA TGGACTTGGG AGTGGGAGTT AACATTGTTG AAACAGCAGC TCTGGGGAGA 
CCCTTCAGGC TGGGGATGCT GTACGACTGC AGAAAAGATG CATTTGTACC AGGAATCACA 
CTGTGGGATG AAGAGCAACT GCAGCAGAGC ATAAAAATCC ATCCTCAAAT TAAAACAGAT 
TTCTGTGTTA CAAATTCAGA CTTTATTGAG GCAAAATGCC AATTACTGAA CATTAATGAT 
TGTCTAAAAC TGAGTTTTTT AAGTGGACTC ATCAATGTTA GTGGAGCAGC CAAGTATCTC 
AGTGACACCA AGAAATCCTT CAAACAGCAG AGACTGACCC TACATTATCA TTCAACCACC 
AAACGTGAAG AACTGACTGT GAACCACTTG CCATCTGAAA ATATAACCCA CTATGAAGTG 
TTTGAGAATG ATGCAGCGAC ACATGTGGTG ACAGCTGTGC TGTATGGGGC CAATGCTTGC 
TTTGTTTTTG ACAGAGAAGC TTCATTAGTT GAAGACCAAA GCACTGTAGA CAGAGACGTA 
AAGGCTGCCT TAGAAAAACT AAAAGGGATT TCAGAAAGTA CACAGATTGA TCTGAGCTTG 
GATGACAATC AAAAGACTGT TTTTGAGAAA TTCAGCTGCA CATTTTATGG TGATTTCCAG 
TTAAAATCTA ATCCAGCCTC TTTTGAAGAA GCTCTGAGGG TTTTTGCTGA TCTTCCTAAA 
CTGCTGGGAG AAACCAAGGA GTGTGCAGTT CCACTGAGAG TGTGGCTTTA TCCACTGGAC 
AAACTGCATT CACATTATGT GAAACTTCAA CAAGAAATCA GCATAGATCT AATCAGAAAT 
GTTGAATCAG TATTTGAGAG CTTAAGGACC ACTGAAATGA AATGCAGTGA TCTTCTGAAT 
GATACACCAT CTTTGGCTTT TGCAGGATTT CATGATAAAA TAATGATTAT GAAGCAAAAC 
TGCTGCAACT ATAAGCTGAG CTTCATGAAG AAACTTGGAT CGCTATTGCC AAAAATCCGT 
AGTGATACAG AAAATGAGAC AGCCCTGATT GAGCTCCTCC ATGATCATGA AAAATGTCCA 
TTCAGAAAAA GGGATCTTGA GAAATGGGTG AAAGAAAAAG AGAATGAATC CGTCATTATT 
AAAACTCTTC TCAGACAATT GACTGATTCT GGAGCAACAG TGGAAAATAA TCTAGATGGA 
ATTTTAATGG ATCTTAATGT TGAAAATGTA GTAAGCTACA CTTTCACATC ACTTGAGTGT 
CCAGATGTGC TCCTTACTAA ACAAAAGGCT TTCCTGAATC CATCAACAAT AGAAAACAAC 
AGTGAGGATG CTTCTGTTTT AAGTAAAAAA TCTGGGTTCA CATCTGACAT CAAAACCACA 
ATGAGGAGCA ACTTGATCAT CTTCAAAAAC TTGATGGAAT CAAAAATATG TAAACCAGCC 
AAATATATTG TTGCATCGAA GGAAATACCA AATCCAGGTT CCTGCATTCT CATTTACGAA 
AATGGATCTG ATGAAGCGAC ATGCTTTACT CCGCCAATAA AACCAGCTCA TCCAGTCACT 
GAACAGATCA GTGGGGAAAG AGTGGTTTTA AAGGTGACAC CTGTGTGTCC TGCAACAGAA 
GAGCTCAGGT TACTGTACAA AACAAAAGAA GAGAAAGACT GGAAATCTCA GTCTGTGAAA 
AAGAGTCAAG ATACAGTTAT TCTGACTGAT CTCAGACCAG ACACTGAGTA TGAGATGAAA 
TTTGCAGCCG TGGGGAAGCT GAACTACACC ATAGACAGTG ATGTGATTCA CCTCAGAGTT 
ATTGACAAAA ACCTCACTGA TGCCACAGAG TCCATTTTAG AAAAGCTGAG CTTGATAGAA 
GACAAGTGCA GTAAACTGAT GGAGGGCAAG AGTGCAGTCA CATTTAGAGC AATTCGCAAC 
AAGTTTGAAG ACATGAAGAG ACACTGTCAG ACTTACAAAC AAGACAGAAT TAAATCCACA 
ATCCAGTCAG TTCAAGCCTG TGAAAAAGAC ATCAGTGCTC TGACAGACCT TCTACAAGCT 
CATCATGAGT CTCCATTTAA CATAAGTGAT ATTGTGGAGT GGATCAGAGT GAAAGAAACC 
GAATCAAAAG CAGTTGAGAA GATTCTTCAA CAGCTGCTGG AAACTGGAGC TGAAGTGAAC 
AATAGTTTAG ATGAATATTT GTTGGATGTT AGTTTTGAGA ATGTGGTGTG TTACACATTC 
ACTTCTCTTG ATCTGCCAGA CGACCTGCTT TCTGATCAAG AAGATTTCCT GAAACCTGAA 
ATGATAAGGA GAAATTCTGA TAAGAAACCC AATGCTGTGT CTGAAACTTG GCTCACAGGA 
AGTACCAGAG AGAAATTGAA AAAACAATTA GAAATGTTTA AAGAGCTCAA ATGTTCATAT 
GGCAGTCACT CAACAAAGTT TTTGGTTATA TCAGAAGATC AGACAAACCA TCCAGGTTCC 
TGTATTCTCC TTTATGAAAA TGGATCTTAT AAAGCGAAAT GCTTTACTCC GCCAATAAAA 
CCAGCTGAGC CCATCATTGA ACAGGTCATC GGGGAAAGAG TGGTTTTGAA GGTGACATCT 
GTGTGTCCTG CAACAGAAGA GCTCAGGTTA CTGTACAAAA CAAAAGAAGA GAACGACTGG 
AAATCTCAGT CTGTGAAAAA GAGTCAAGAT ACAGTTATTC TGACTGATCT CAGACCAGAC 
ACTGAGTATG AGATGAAATT TGCAGCTGTG GGGAAGCTGA ACTACACCAT AGACAGTGAT 
GTGATTCACC TTAGAGTTAT TGACAAGAAA CTCACTGATG CCACAGAGTC CATTTTAGAA 
AAGCTGAGCT TGATAGAAGA CAAGTGCAGT AAACTGATGG AGGACAAGAG TGCAGTCACT 
TTTAGAGCAA TTCACAACAA GTTTGAAGAC ATGAGAAGAC ACTGTCAGAT CTACAAACAA 
GACTTAGAAA TTAAAATTAC ATCCACAATG CAGTCAGTTC AAACCTGTGA AAAAGACATC 
AGTGCTCTGA CAGACCTTCT ACAAGCTCAT CATGAGTCTC CATTTAATGA AAGTGATCTT 
ATAGAGTGGA TCAGAGTGAA AGAAACCGAA TCAAATGCAG TTGAGAAGAT TCTTCAACAG 
CTGCTGGACT CTGGAACTGA GGTGAACAAT AGTTTAGATG AATATTTGTT TGATGTTAGT 
TTTGAGAATG TGGTGTGTTA CACATTCACT TCTCTTGATC TGCCAGACAA CCTGCTTTCT 
AAACAAGGAG ATTTCCTGAA ACCTGAAATA ATGAGGAGAA ATTCTAATAA GAACCCCAAT 
TCTGTGTCTG AAACTTGGCT CACAGGAAGT ATCAGAGAGA CTATGAAGAA GCATTTGGAA 
ATATTTAATG AGCTCAAATG TTCATATGGC AGTCACTCAT CAACATTTTT GGTTGTATAT 
AAAAGTCACA CAAACCATCC AGGTTCCTGC ATTCTTATTT ATGAAAATGG ATCTGATAAA 
GCGACATGCT TCACTCCTCC AATAAAACCA GCTAGTCCAG TCATTGAACA GATTGGTGAG 
GATATAGTGG TTTTGAAGGC AACACCATTA TGTCCTGCAA CACAAGAGCT CAGGTTACTG 
TACAAAAAAA AAGAAGAGAA TGATTGGAAA TCTCAGTCTG TGAAAACGAG TGGAGATGAA 
GTTTTTCTGA CTGGTCTCAG ACCTGACACT GAGTATAAGA TGAAATATGT GGCCTTGGGG 
AAGCTGAACT ACACCACAGA CAGTGATGTC ATCGGAGTTA CTACACGTGT GCTAAATGAG 
ACTGGCAAAT ATCTGCAGGA TAAATCTGAA CAAAAACTAT CGATGACAAA GGAGCCAAGG 
AACAACCAAC CAATGTCATT TGATTCTTCA GAGGAGGTCA AAGTGTGCAC TGTTTTGGCT 
GGAAAGACCA ATAACTGTCA AACACATTTT ATTGCATCAC TGCGAGCCCG AATTGAGCAT 
CTGACGGAAG TTCGTTCAGT AGACGAGAGT GACATTGTTT TAATTTTCTG TCCAATTGTT 
TCTCGAGCTG GAACTGACAT TGATGCAGCC ATTCACAGAT GCTATACAAA TGCCTCTAAG 
CTGACTGTTC TAGTGGTGCT TCACCACACG TTTGACCCAG AGAAAACTGT ATCAGACAGC 
AGCAAAATTG TCAACAGGGA GAATCTGCTC ACAGTGGACT GTCTGTTCTA TGAGGATGCA 
GGATTACTGC AGTGTCAGAG GAATTCAGAT GCATATGACA AATCTGTCAA CTGGTTAATA 
GCGCAGGGCA AAAAAACAGG TCTTAAAATC TACCCAAAGA AATCACCTGA TAGTCAATCC 
AATGGTCGGA AATTCTGGAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332126.1
CDS	301..4644
Translation	MRSMDLGVGVNIVETAALGRPFRLGMLYDCRKDAFVPGITLWDEEQLQQSIKIHPQIKTDFCVTNSDFIEAKCQLLNINDCLKLSFLSGLINVSGAAKYLSDTKKSFKQQRLTLHYHSTTKREELTVNHLPSENITHYEVFENDAATHVVTAVLYGANACFVFDREASLVEDQSTVDRDVKAALEKLKGISESTQIDLSLDDNQKTVFEKFSCTFYGDFQLKSNPASFEEALRVFADLPKLLGETKECAVPLRVWLYPLDKLHSHYVKLQQEISIDLIRNVESVFESLRTTEMKCSDLLNDTPSLAFAGFHDKIMIMKQNCCNYKLSFMKKLGSLLPKIRSDTENETALIELLHDHEKCPFRKRDLEKWVKEKENESVIIKTLLRQLTDSGATVENNLDGILMDLNVENVVSYTFTSLECPDVLLTKQKAFLNPSTIENNSEDASVLSKKSGFTSDIKTTMRSNLIIFKNLMESKICKPAKYIVASKEIPNPGSCILIYENGSDEATCFTPPIKPAHPVTEQISGERVVLKVTPVCPATEELRLLYKTKEEKDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKNLTDATESILEKLSLIEDKCSKLMEGKSAVTFRAIRNKFEDMKRHCQTYKQDRIKSTIQSVQACEKDISALTDLLQAHHESPFNISDIVEWIRVKETESKAVEKILQQLLETGAEVNNSLDEYLLDVSFENVVCYTFTSLDLPDDLLSDQEDFLKPEMIRRNSDKKPNAVSETWLTGSTREKLKKQLEMFKELKCSYGSHSTKFLVISEDQTNHPGSCILLYENGSYKAKCFTPPIKPAEPIIEQVIGERVVLKVTSVCPATEELRLLYKTKEENDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKKLTDATESILEKLSLIEDKCSKLMEDKSAVTFRAIHNKFEDMRRHCQIYKQDLEIKITSTMQSVQTCEKDISALTDLLQAHHESPFNESDLIEWIRVKETESNAVEKILQQLLDSGTEVNNSLDEYLFDVSFENVVCYTFTSLDLPDNLLSKQGDFLKPEIMRRNSNKNPNSVSETWLTGSIRETMKKHLEIFNELKCSYGSHSSTFLVVYKSHTNHPGSCILIYENGSDKATCFTPPIKPASPVIEQIGEDIVVLKATPLCPATQELRLLYKKKEENDWKSQSVKTSGDEVFLTGLRPDTEYKMKYVALGKLNYTTDSDVIGVTTRVLNETGKYLQDKSEQKLSMTKEPRNNQPMSFDSSEEVKVCTVLAGKTNNCQTHFIASLRARIEHLTEVRSVDESDIVLIFCPIVSRAGTDIDAAIHRCYTNASKLTVLVVLHHTFDPEKTVSDSSKIVNRENLLTVDCLFYEDAGLLQCQRNSDAYDKSVNWLIAQGKKTGLKIYPKKSPDSQSNGRKFWR

Target ORF information:

RefSeq Version	XM_021476451.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476451.1

ORF Insert Sequence:

ATGCGCAGTA TGGACTTGGG AGTGGGAGTT AACATTGTTG AAACAGCAGC TCTGGGGAGA 
CCCTTCAGGC TGGGGATGCT GTACGACTGC AGAAAAGATG CATTTGTACC AGGAATCACA 
CTGTGGGATG AAGAGCAACT GCAGCAGAGC ATAAAAATCC ATCCTCAAAT TAAAACAGAT 
TTCTGTGTTA CAAATTCAGA CTTTATTGAG GCAAAATGCC AATTACTGAA CATTAATGAT 
TGTCTAAAAC TGAGTTTTTT AAGTGGACTC ATCAATGTTA GTGGAGCAGC CAAGTATCTC 
AGTGACACCA AGAAATCCTT CAAACAGCAG AGACTGACCC TACATTATCA TTCAACCACC 
AAACGTGAAG AACTGACTGT GAACCACTTG CCATCTGAAA ATATAACCCA CTATGAAGTG 
TTTGAGAATG ATGCAGCGAC ACATGTGGTG ACAGCTGTGC TGTATGGGGC CAATGCTTGC 
TTTGTTTTTG ACAGAGAAGC TTCATTAGTT GAAGACCAAA GCACTGTAGA CAGAGACGTA 
AAGGCTGCCT TAGAAAAACT AAAAGGGATT TCAGAAAGTA CACAGATTGA TCTGAGCTTG 
GATGACAATC AAAAGACTGT TTTTGAGAAA TTCAGCTGCA CATTTTATGG TGATTTCCAG 
TTAAAATCTA ATCCAGCCTC TTTTGAAGAA GCTCTGAGGG TTTTTGCTGA TCTTCCTAAA 
CTGCTGGGAG AAACCAAGGA GTGTGCAGTT CCACTGAGAG TGTGGCTTTA TCCACTGGAC 
AAACTGCATT CACATTATGT GAAACTTCAA CAAGAAATCA GCATAGATCT AATCAGAAAT 
GTTGAATCAG TATTTGAGAG CTTAAGGACC ACTGAAATGA AATGCAGTGA TCTTCTGAAT 
GATACACCAT CTTTGGCTTT TGCAGGATTT CATGATAAAA TAATGATTAT GAAGCAAAAC 
TGCTGCAACT ATAAGCTGAG CTTCATGAAG AAACTTGGAT CGCTATTGCC AAAAATCCGT 
AGTGATACAG AAAATGAGAC AGCCCTGATT GAGCTCCTCC ATGATCATGA AAAATGTCCA 
TTCAGAAAAA GGGATCTTGA GAAATGGGTG AAAGAAAAAG AGAATGAATC CGTCATTATT 
AAAACTCTTC TCAGACAATT GACTGATTCT GGAGCAACAG TGGAAAATAA TCTAGATGGA 
ATTTTAATGG ATCTTAATGT TGAAAATGTA GTAAGCTACA CTTTCACATC ACTTGAGTGT 
CCAGATGTGC TCCTTACTAA ACAAAAGGCT TTCCTGAATC CATCAACAAT AGAAAACAAC 
AGTGAGGATG CTTCTGTTTT AAGTAAAAAA TCTGGGTTCA CATCTGACAT CAAAACCACA 
ATGAGGAGCA ACTTGATCAT CTTCAAAAAC TTGATGGAAT CAAAAATATG TAAACCAGCC 
AAATATATTG TTGCATCGAA GGAAATACCA AATCCAGGTT CCTGCATTCT CATTTACGAA 
AATGGATCTG ATGAAGCGAC ATGCTTTACT CCGCCAATAA AACCAGCTCA TCCAGTCACT 
GAACAGATCA GTGGGGAAAG AGTGGTTTTA AAGGTGACAC CTGTGTGTCC TGCAACAGAA 
GAGCTCAGGT TACTGTACAA AACAAAAGAA GAGAAAGACT GGAAATCTCA GTCTGTGAAA 
AAGAGTCAAG ATACAGTTAT TCTGACTGAT CTCAGACCAG ACACTGAGTA TGAGATGAAA 
TTTGCAGCCG TGGGGAAGCT GAACTACACC ATAGACAGTG ATGTGATTCA CCTCAGAGTT 
ATTGACAAAA ACCTCACTGA TGCCACAGAG TCCATTTTAG AAAAGCTGAG CTTGATAGAA 
GACAAGTGCA GTAAACTGAT GGAGGGCAAG AGTGCAGTCA CATTTAGAGC AATTCGCAAC 
AAGTTTGAAG ACATGAAGAG ACACTGTCAG ACTTACAAAC AAGACAGAAT TAAATCCACA 
ATCCAGTCAG TTCAAGCCTG TGAAAAAGAC ATCAGTGCTC TGACAGACCT TCTACAAGCT 
CATCATGAGT CTCCATTTAA CATAAGTGAT ATTGTGGAGT GGATCAGAGT GAAAGAAACC 
GAATCAAAAG CAGTTGAGAA GATTCTTCAA CAGCTGCTGG AAACTGGAGC TGAAGTGAAC 
AATAGTTTAG ATGAATATTT GTTGGATGTT AGTTTTGAGA ATGTGGTGTG TTACACATTC 
ACTTCTCTTG ATCTGCCAGA CGACCTGCTT TCTGATCAAG AAGATTTCCT GAAACCTGAA 
ATGATAAGGA GAAATTCTGA TAAGAAACCC AATGCTGTGT CTGAAACTTG GCTCACAGGA 
AGTACCAGAG AGAAATTGAA AAAACAATTA GAAATGTTTA AAGAGCTCAA ATGTTCATAT 
GGCAGTCACT CAACAAAGTT TTTGGTTATA TCAGAAGATC AGACAAACCA TCCAGGTTCC 
TGTATTCTCC TTTATGAAAA TGGATCTTAT AAAGCGAAAT GCTTTACTCC GCCAATAAAA 
CCAGCTGAGC CCATCATTGA ACAGGTCATC GGGGAAAGAG TGGTTTTGAA GGTGACATCT 
GTGTGTCCTG CAACAGAAGA GCTCAGGTTA CTGTACAAAA CAAAAGAAGA GAACGACTGG 
AAATCTCAGT CTGTGAAAAA GAGTCAAGAT ACAGTTATTC TGACTGATCT CAGACCAGAC 
ACTGAGTATG AGATGAAATT TGCAGCTGTG GGGAAGCTGA ACTACACCAT AGACAGTGAT 
GTGATTCACC TTAGAGTTAT TGACAAGAAA CTCACTGATG CCACAGAGTC CATTTTAGAA 
AAGCTGAGCT TGATAGAAGA CAAGTGCAGT AAACTGATGG AGGACAAGAG TGCAGTCACT 
TTTAGAGCAA TTCACAACAA GTTTGAAGAC ATGAGAAGAC ACTGTCAGAT CTACAAACAA 
GACTTAGAAA TTAAAATTAC ATCCACAATG CAGTCAGTTC AAACCTGTGA AAAAGACATC 
AGTGCTCTGA CAGACCTTCT ACAAGCTCAT CATGAGTCTC CATTTAATGA AAGTGATCTT 
ATAGAGTGGA TCAGAGTGAA AGAAACCGAA TCAAATGCAG TTGAGAAGAT TCTTCAACAG 
CTGCTGGACT CTGGAACTGA GGTGAACAAT AGTTTAGATG AATATTTGTT TGATGTTAGT 
TTTGAGAATG TGGTGTGTTA CACATTCACT TCTCTTGATC TGCCAGACAA CCTGCTTTCT 
AAACAAGGAG ATTTCCTGAA ACCTGAAATA ATGAGGAGAA ATTCTAATAA GAACCCCAAT 
TCTGTGTCTG AAACTTGGCT CACAGGAAGT ATCAGAGAGA CTATGAAGAA GCATTTGGAA 
ATATTTAATG AGCTCAAATG TTCATATGGC AGTCACTCAT CAACATTTTT GGTTGTATAT 
AAAAGTCACA CAAACCATCC AGGTTCCTGC ATTCTTATTT ATGAAAATGG ATCTGATAAA 
GCGACATGCT TCACTCCTCC AATAAAACCA GCTAGTCCAG TCATTGAACA GATTGGTGAG 
GATATAGTGG TTTTGAAGGC AACACCATTA TGTCCTGCAA CACAAGAGCT CAGGTTACTG 
TACAAAAAAA AAGAAGAGAA TGATTGGAAA TCTCAGTCTG TGAAAACGAG TGGAGATGAA 
GTTTTTCTGA CTGGTCTCAG ACCTGACACT GAGTATAAGA TGAAATATGT GGCCTTGGGG 
AAGCTGAACT ACACCACAGA CAGTGATGTC ATCGGAGTTA CTACACGTGT GCTAAATGAG 
ACTGGCAAAT ATCTGCAGGA TAAATCTGAA CAAAAACTAT CGATGACAAA GGAGCCAAGG 
AACAACCAAC CAATGTCATT TGATTCTTCA GAGGAGGTCA AAGTGTGCAC TGTTTTGGCT 
GGAAAGACCA ATAACTGTCA AACACATTTT ATTGCATCAC TGCGAGCCCG AATTGAGCAT 
CTGACGGAAG TTCGTTCAGT AGACGAGAGT GACATTGTTT TAATTTTCTG TCCAATTGTT 
TCTCGAGCTG GAACTGACAT TGATGCAGCC ATTCACAGAT GCTATACAAA TGCCTCTAAG 
CTGACTGTTC TAGTGGTGCT TCACCACACG TTTGACCCAG AGAAAACTGT ATCAGACAGC 
AGCAAAATTG TCAACAGGGA GAATCTGCTC ACAGTGGACT GTCTGTTCTA TGAGGATGCA 
GGATTACTGC AGTGTCAGAG GAATTCAGAT GCATATGACA AATCTGTCAA CTGGTTAATA 
GCGCAGGGCA AAAAAACAGG TCTTAAAATC TACCCAAAGA AATCACCTGA TAGTCAATCC 
AATGGTCGGA AATTCTGGAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100148510 ( XM_021471583.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327258.1 Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa142778
Clone ID Related Accession (Same CDS sequence)	XM_021476451.1 , XM_021471583.1
Accession Version	XM_021471583.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC100148510 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAGTA TGGACTTGGG AGTGGGAGTT AACATTGTTG AAACAGCAGC TCTGGGGAGA 
CCCTTCAGGC TGGGGATGCT GTACGACTGC AGAAAAGATG CATTTGTACC AGGAATCACA 
CTGTGGGATG AAGAGCAACT GCAGCAGAGC ATAAAAATCC ATCCTCAAAT TAAAACAGAT 
TTCTGTGTTA CAAATTCAGA CTTTATTGAG GCAAAATGCC AATTACTGAA CATTAATGAT 
TGTCTAAAAC TGAGTTTTTT AAGTGGACTC ATCAATGTTA GTGGAGCAGC CAAGTATCTC 
AGTGACACCA AGAAATCCTT CAAACAGCAG AGACTGACCC TACATTATCA TTCAACCACC 
AAACGTGAAG AACTGACTGT GAACCACTTG CCATCTGAAA ATATAACCCA CTATGAAGTG 
TTTGAGAATG ATGCAGCGAC ACATGTGGTG ACAGCTGTGC TGTATGGGGC CAATGCTTGC 
TTTGTTTTTG ACAGAGAAGC TTCATTAGTT GAAGACCAAA GCACTGTAGA CAGAGACGTA 
AAGGCTGCCT TAGAAAAACT AAAAGGGATT TCAGAAAGTA CACAGATTGA TCTGAGCTTG 
GATGACAATC AAAAGACTGT TTTTGAGAAA TTCAGCTGCA CATTTTATGG TGATTTCCAG 
TTAAAATCTA ATCCAGCCTC TTTTGAAGAA GCTCTGAGGG TTTTTGCTGA TCTTCCTAAA 
CTGCTGGGAG AAACCAAGGA GTGTGCAGTT CCACTGAGAG TGTGGCTTTA TCCACTGGAC 
AAACTGCATT CACATTATGT GAAACTTCAA CAAGAAATCA GCATAGATCT AATCAGAAAT 
GTTGAATCAG TATTTGAGAG CTTAAGGACC ACTGAAATGA AATGCAGTGA TCTTCTGAAT 
GATACACCAT CTTTGGCTTT TGCAGGATTT CATGATAAAA TAATGATTAT GAAGCAAAAC 
TGCTGCAACT ATAAGCTGAG CTTCATGAAG AAACTTGGAT CGCTATTGCC AAAAATCCGT 
AGTGATACAG AAAATGAGAC AGCCCTGATT GAGCTCCTCC ATGATCATGA AAAATGTCCA 
TTCAGAAAAA GGGATCTTGA GAAATGGGTG AAAGAAAAAG AGAATGAATC CGTCATTATT 
AAAACTCTTC TCAGACAATT GACTGATTCT GGAGCAACAG TGGAAAATAA TCTAGATGGA 
ATTTTAATGG ATCTTAATGT TGAAAATGTA GTAAGCTACA CTTTCACATC ACTTGAGTGT 
CCAGATGTGC TCCTTACTAA ACAAAAGGCT TTCCTGAATC CATCAACAAT AGAAAACAAC 
AGTGAGGATG CTTCTGTTTT AAGTAAAAAA TCTGGGTTCA CATCTGACAT CAAAACCACA 
ATGAGGAGCA ACTTGATCAT CTTCAAAAAC TTGATGGAAT CAAAAATATG TAAACCAGCC 
AAATATATTG TTGCATCGAA GGAAATACCA AATCCAGGTT CCTGCATTCT CATTTACGAA 
AATGGATCTG ATGAAGCGAC ATGCTTTACT CCGCCAATAA AACCAGCTCA TCCAGTCACT 
GAACAGATCA GTGGGGAAAG AGTGGTTTTA AAGGTGACAC CTGTGTGTCC TGCAACAGAA 
GAGCTCAGGT TACTGTACAA AACAAAAGAA GAGAAAGACT GGAAATCTCA GTCTGTGAAA 
AAGAGTCAAG ATACAGTTAT TCTGACTGAT CTCAGACCAG ACACTGAGTA TGAGATGAAA 
TTTGCAGCCG TGGGGAAGCT GAACTACACC ATAGACAGTG ATGTGATTCA CCTCAGAGTT 
ATTGACAAAA ACCTCACTGA TGCCACAGAG TCCATTTTAG AAAAGCTGAG CTTGATAGAA 
GACAAGTGCA GTAAACTGAT GGAGGGCAAG AGTGCAGTCA CATTTAGAGC AATTCGCAAC 
AAGTTTGAAG ACATGAAGAG ACACTGTCAG ACTTACAAAC AAGACAGAAT TAAATCCACA 
ATCCAGTCAG TTCAAGCCTG TGAAAAAGAC ATCAGTGCTC TGACAGACCT TCTACAAGCT 
CATCATGAGT CTCCATTTAA CATAAGTGAT ATTGTGGAGT GGATCAGAGT GAAAGAAACC 
GAATCAAAAG CAGTTGAGAA GATTCTTCAA CAGCTGCTGG AAACTGGAGC TGAAGTGAAC 
AATAGTTTAG ATGAATATTT GTTGGATGTT AGTTTTGAGA ATGTGGTGTG TTACACATTC 
ACTTCTCTTG ATCTGCCAGA CGACCTGCTT TCTGATCAAG AAGATTTCCT GAAACCTGAA 
ATGATAAGGA GAAATTCTGA TAAGAAACCC AATGCTGTGT CTGAAACTTG GCTCACAGGA 
AGTACCAGAG AGAAATTGAA AAAACAATTA GAAATGTTTA AAGAGCTCAA ATGTTCATAT 
GGCAGTCACT CAACAAAGTT TTTGGTTATA TCAGAAGATC AGACAAACCA TCCAGGTTCC 
TGTATTCTCC TTTATGAAAA TGGATCTTAT AAAGCGAAAT GCTTTACTCC GCCAATAAAA 
CCAGCTGAGC CCATCATTGA ACAGGTCATC GGGGAAAGAG TGGTTTTGAA GGTGACATCT 
GTGTGTCCTG CAACAGAAGA GCTCAGGTTA CTGTACAAAA CAAAAGAAGA GAACGACTGG 
AAATCTCAGT CTGTGAAAAA GAGTCAAGAT ACAGTTATTC TGACTGATCT CAGACCAGAC 
ACTGAGTATG AGATGAAATT TGCAGCTGTG GGGAAGCTGA ACTACACCAT AGACAGTGAT 
GTGATTCACC TTAGAGTTAT TGACAAGAAA CTCACTGATG CCACAGAGTC CATTTTAGAA 
AAGCTGAGCT TGATAGAAGA CAAGTGCAGT AAACTGATGG AGGACAAGAG TGCAGTCACT 
TTTAGAGCAA TTCACAACAA GTTTGAAGAC ATGAGAAGAC ACTGTCAGAT CTACAAACAA 
GACTTAGAAA TTAAAATTAC ATCCACAATG CAGTCAGTTC AAACCTGTGA AAAAGACATC 
AGTGCTCTGA CAGACCTTCT ACAAGCTCAT CATGAGTCTC CATTTAATGA AAGTGATCTT 
ATAGAGTGGA TCAGAGTGAA AGAAACCGAA TCAAATGCAG TTGAGAAGAT TCTTCAACAG 
CTGCTGGACT CTGGAACTGA GGTGAACAAT AGTTTAGATG AATATTTGTT TGATGTTAGT 
TTTGAGAATG TGGTGTGTTA CACATTCACT TCTCTTGATC TGCCAGACAA CCTGCTTTCT 
AAACAAGGAG ATTTCCTGAA ACCTGAAATA ATGAGGAGAA ATTCTAATAA GAACCCCAAT 
TCTGTGTCTG AAACTTGGCT CACAGGAAGT ATCAGAGAGA CTATGAAGAA GCATTTGGAA 
ATATTTAATG AGCTCAAATG TTCATATGGC AGTCACTCAT CAACATTTTT GGTTGTATAT 
AAAAGTCACA CAAACCATCC AGGTTCCTGC ATTCTTATTT ATGAAAATGG ATCTGATAAA 
GCGACATGCT TCACTCCTCC AATAAAACCA GCTAGTCCAG TCATTGAACA GATTGGTGAG 
GATATAGTGG TTTTGAAGGC AACACCATTA TGTCCTGCAA CACAAGAGCT CAGGTTACTG 
TACAAAAAAA AAGAAGAGAA TGATTGGAAA TCTCAGTCTG TGAAAACGAG TGGAGATGAA 
GTTTTTCTGA CTGGTCTCAG ACCTGACACT GAGTATAAGA TGAAATATGT GGCCTTGGGG 
AAGCTGAACT ACACCACAGA CAGTGATGTC ATCGGAGTTA CTACACGTGT GCTAAATGAG 
ACTGGCAAAT ATCTGCAGGA TAAATCTGAA CAAAAACTAT CGATGACAAA GGAGCCAAGG 
AACAACCAAC CAATGTCATT TGATTCTTCA GAGGAGGTCA AAGTGTGCAC TGTTTTGGCT 
GGAAAGACCA ATAACTGTCA AACACATTTT ATTGCATCAC TGCGAGCCCG AATTGAGCAT 
CTGACGGAAG TTCGTTCAGT AGACGAGAGT GACATTGTTT TAATTTTCTG TCCAATTGTT 
TCTCGAGCTG GAACTGACAT TGATGCAGCC ATTCACAGAT GCTATACAAA TGCCTCTAAG 
CTGACTGTTC TAGTGGTGCT TCACCACACG TTTGACCCAG AGAAAACTGT ATCAGACAGC 
AGCAAAATTG TCAACAGGGA GAATCTGCTC ACAGTGGACT GTCTGTTCTA TGAGGATGCA 
GGATTACTGC AGTGTCAGAG GAATTCAGAT GCATATGACA AATCTGTCAA CTGGTTAATA 
GCGCAGGGCA AAAAAACAGG TCTTAAAATC TACCCAAAGA AATCACCTGA TAGTCAATCC 
AATGGTCGGA AATTCTGGAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327258.1
CDS	301..4644
Translation	MRSMDLGVGVNIVETAALGRPFRLGMLYDCRKDAFVPGITLWDEEQLQQSIKIHPQIKTDFCVTNSDFIEAKCQLLNINDCLKLSFLSGLINVSGAAKYLSDTKKSFKQQRLTLHYHSTTKREELTVNHLPSENITHYEVFENDAATHVVTAVLYGANACFVFDREASLVEDQSTVDRDVKAALEKLKGISESTQIDLSLDDNQKTVFEKFSCTFYGDFQLKSNPASFEEALRVFADLPKLLGETKECAVPLRVWLYPLDKLHSHYVKLQQEISIDLIRNVESVFESLRTTEMKCSDLLNDTPSLAFAGFHDKIMIMKQNCCNYKLSFMKKLGSLLPKIRSDTENETALIELLHDHEKCPFRKRDLEKWVKEKENESVIIKTLLRQLTDSGATVENNLDGILMDLNVENVVSYTFTSLECPDVLLTKQKAFLNPSTIENNSEDASVLSKKSGFTSDIKTTMRSNLIIFKNLMESKICKPAKYIVASKEIPNPGSCILIYENGSDEATCFTPPIKPAHPVTEQISGERVVLKVTPVCPATEELRLLYKTKEEKDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKNLTDATESILEKLSLIEDKCSKLMEGKSAVTFRAIRNKFEDMKRHCQTYKQDRIKSTIQSVQACEKDISALTDLLQAHHESPFNISDIVEWIRVKETESKAVEKILQQLLETGAEVNNSLDEYLLDVSFENVVCYTFTSLDLPDDLLSDQEDFLKPEMIRRNSDKKPNAVSETWLTGSTREKLKKQLEMFKELKCSYGSHSTKFLVISEDQTNHPGSCILLYENGSYKAKCFTPPIKPAEPIIEQVIGERVVLKVTSVCPATEELRLLYKTKEENDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKKLTDATESILEKLSLIEDKCSKLMEDKSAVTFRAIHNKFEDMRRHCQIYKQDLEIKITSTMQSVQTCEKDISALTDLLQAHHESPFNESDLIEWIRVKETESNAVEKILQQLLDSGTEVNNSLDEYLFDVSFENVVCYTFTSLDLPDNLLSKQGDFLKPEIMRRNSNKNPNSVSETWLTGSIRETMKKHLEIFNELKCSYGSHSSTFLVVYKSHTNHPGSCILIYENGSDKATCFTPPIKPASPVIEQIGEDIVVLKATPLCPATQELRLLYKKKEENDWKSQSVKTSGDEVFLTGLRPDTEYKMKYVALGKLNYTTDSDVIGVTTRVLNETGKYLQDKSEQKLSMTKEPRNNQPMSFDSSEEVKVCTVLAGKTNNCQTHFIASLRARIEHLTEVRSVDESDIVLIFCPIVSRAGTDIDAAIHRCYTNASKLTVLVVLHHTFDPEKTVSDSSKIVNRENLLTVDCLFYEDAGLLQCQRNSDAYDKSVNWLIAQGKKTGLKIYPKKSPDSQSNGRKFWR

Target ORF information:

RefSeq Version	XM_021471583.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471583.1

ORF Insert Sequence:

ATGCGCAGTA TGGACTTGGG AGTGGGAGTT AACATTGTTG AAACAGCAGC TCTGGGGAGA 
CCCTTCAGGC TGGGGATGCT GTACGACTGC AGAAAAGATG CATTTGTACC AGGAATCACA 
CTGTGGGATG AAGAGCAACT GCAGCAGAGC ATAAAAATCC ATCCTCAAAT TAAAACAGAT 
TTCTGTGTTA CAAATTCAGA CTTTATTGAG GCAAAATGCC AATTACTGAA CATTAATGAT 
TGTCTAAAAC TGAGTTTTTT AAGTGGACTC ATCAATGTTA GTGGAGCAGC CAAGTATCTC 
AGTGACACCA AGAAATCCTT CAAACAGCAG AGACTGACCC TACATTATCA TTCAACCACC 
AAACGTGAAG AACTGACTGT GAACCACTTG CCATCTGAAA ATATAACCCA CTATGAAGTG 
TTTGAGAATG ATGCAGCGAC ACATGTGGTG ACAGCTGTGC TGTATGGGGC CAATGCTTGC 
TTTGTTTTTG ACAGAGAAGC TTCATTAGTT GAAGACCAAA GCACTGTAGA CAGAGACGTA 
AAGGCTGCCT TAGAAAAACT AAAAGGGATT TCAGAAAGTA CACAGATTGA TCTGAGCTTG 
GATGACAATC AAAAGACTGT TTTTGAGAAA TTCAGCTGCA CATTTTATGG TGATTTCCAG 
TTAAAATCTA ATCCAGCCTC TTTTGAAGAA GCTCTGAGGG TTTTTGCTGA TCTTCCTAAA 
CTGCTGGGAG AAACCAAGGA GTGTGCAGTT CCACTGAGAG TGTGGCTTTA TCCACTGGAC 
AAACTGCATT CACATTATGT GAAACTTCAA CAAGAAATCA GCATAGATCT AATCAGAAAT 
GTTGAATCAG TATTTGAGAG CTTAAGGACC ACTGAAATGA AATGCAGTGA TCTTCTGAAT 
GATACACCAT CTTTGGCTTT TGCAGGATTT CATGATAAAA TAATGATTAT GAAGCAAAAC 
TGCTGCAACT ATAAGCTGAG CTTCATGAAG AAACTTGGAT CGCTATTGCC AAAAATCCGT 
AGTGATACAG AAAATGAGAC AGCCCTGATT GAGCTCCTCC ATGATCATGA AAAATGTCCA 
TTCAGAAAAA GGGATCTTGA GAAATGGGTG AAAGAAAAAG AGAATGAATC CGTCATTATT 
AAAACTCTTC TCAGACAATT GACTGATTCT GGAGCAACAG TGGAAAATAA TCTAGATGGA 
ATTTTAATGG ATCTTAATGT TGAAAATGTA GTAAGCTACA CTTTCACATC ACTTGAGTGT 
CCAGATGTGC TCCTTACTAA ACAAAAGGCT TTCCTGAATC CATCAACAAT AGAAAACAAC 
AGTGAGGATG CTTCTGTTTT AAGTAAAAAA TCTGGGTTCA CATCTGACAT CAAAACCACA 
ATGAGGAGCA ACTTGATCAT CTTCAAAAAC TTGATGGAAT CAAAAATATG TAAACCAGCC 
AAATATATTG TTGCATCGAA GGAAATACCA AATCCAGGTT CCTGCATTCT CATTTACGAA 
AATGGATCTG ATGAAGCGAC ATGCTTTACT CCGCCAATAA AACCAGCTCA TCCAGTCACT 
GAACAGATCA GTGGGGAAAG AGTGGTTTTA AAGGTGACAC CTGTGTGTCC TGCAACAGAA 
GAGCTCAGGT TACTGTACAA AACAAAAGAA GAGAAAGACT GGAAATCTCA GTCTGTGAAA 
AAGAGTCAAG ATACAGTTAT TCTGACTGAT CTCAGACCAG ACACTGAGTA TGAGATGAAA 
TTTGCAGCCG TGGGGAAGCT GAACTACACC ATAGACAGTG ATGTGATTCA CCTCAGAGTT 
ATTGACAAAA ACCTCACTGA TGCCACAGAG TCCATTTTAG AAAAGCTGAG CTTGATAGAA 
GACAAGTGCA GTAAACTGAT GGAGGGCAAG AGTGCAGTCA CATTTAGAGC AATTCGCAAC 
AAGTTTGAAG ACATGAAGAG ACACTGTCAG ACTTACAAAC AAGACAGAAT TAAATCCACA 
ATCCAGTCAG TTCAAGCCTG TGAAAAAGAC ATCAGTGCTC TGACAGACCT TCTACAAGCT 
CATCATGAGT CTCCATTTAA CATAAGTGAT ATTGTGGAGT GGATCAGAGT GAAAGAAACC 
GAATCAAAAG CAGTTGAGAA GATTCTTCAA CAGCTGCTGG AAACTGGAGC TGAAGTGAAC 
AATAGTTTAG ATGAATATTT GTTGGATGTT AGTTTTGAGA ATGTGGTGTG TTACACATTC 
ACTTCTCTTG ATCTGCCAGA CGACCTGCTT TCTGATCAAG AAGATTTCCT GAAACCTGAA 
ATGATAAGGA GAAATTCTGA TAAGAAACCC AATGCTGTGT CTGAAACTTG GCTCACAGGA 
AGTACCAGAG AGAAATTGAA AAAACAATTA GAAATGTTTA AAGAGCTCAA ATGTTCATAT 
GGCAGTCACT CAACAAAGTT TTTGGTTATA TCAGAAGATC AGACAAACCA TCCAGGTTCC 
TGTATTCTCC TTTATGAAAA TGGATCTTAT AAAGCGAAAT GCTTTACTCC GCCAATAAAA 
CCAGCTGAGC CCATCATTGA ACAGGTCATC GGGGAAAGAG TGGTTTTGAA GGTGACATCT 
GTGTGTCCTG CAACAGAAGA GCTCAGGTTA CTGTACAAAA CAAAAGAAGA GAACGACTGG 
AAATCTCAGT CTGTGAAAAA GAGTCAAGAT ACAGTTATTC TGACTGATCT CAGACCAGAC 
ACTGAGTATG AGATGAAATT TGCAGCTGTG GGGAAGCTGA ACTACACCAT AGACAGTGAT 
GTGATTCACC TTAGAGTTAT TGACAAGAAA CTCACTGATG CCACAGAGTC CATTTTAGAA 
AAGCTGAGCT TGATAGAAGA CAAGTGCAGT AAACTGATGG AGGACAAGAG TGCAGTCACT 
TTTAGAGCAA TTCACAACAA GTTTGAAGAC ATGAGAAGAC ACTGTCAGAT CTACAAACAA 
GACTTAGAAA TTAAAATTAC ATCCACAATG CAGTCAGTTC AAACCTGTGA AAAAGACATC 
AGTGCTCTGA CAGACCTTCT ACAAGCTCAT CATGAGTCTC CATTTAATGA AAGTGATCTT 
ATAGAGTGGA TCAGAGTGAA AGAAACCGAA TCAAATGCAG TTGAGAAGAT TCTTCAACAG 
CTGCTGGACT CTGGAACTGA GGTGAACAAT AGTTTAGATG AATATTTGTT TGATGTTAGT 
TTTGAGAATG TGGTGTGTTA CACATTCACT TCTCTTGATC TGCCAGACAA CCTGCTTTCT 
AAACAAGGAG ATTTCCTGAA ACCTGAAATA ATGAGGAGAA ATTCTAATAA GAACCCCAAT 
TCTGTGTCTG AAACTTGGCT CACAGGAAGT ATCAGAGAGA CTATGAAGAA GCATTTGGAA 
ATATTTAATG AGCTCAAATG TTCATATGGC AGTCACTCAT CAACATTTTT GGTTGTATAT 
AAAAGTCACA CAAACCATCC AGGTTCCTGC ATTCTTATTT ATGAAAATGG ATCTGATAAA 
GCGACATGCT TCACTCCTCC AATAAAACCA GCTAGTCCAG TCATTGAACA GATTGGTGAG 
GATATAGTGG TTTTGAAGGC AACACCATTA TGTCCTGCAA CACAAGAGCT CAGGTTACTG 
TACAAAAAAA AAGAAGAGAA TGATTGGAAA TCTCAGTCTG TGAAAACGAG TGGAGATGAA 
GTTTTTCTGA CTGGTCTCAG ACCTGACACT GAGTATAAGA TGAAATATGT GGCCTTGGGG 
AAGCTGAACT ACACCACAGA CAGTGATGTC ATCGGAGTTA CTACACGTGT GCTAAATGAG 
ACTGGCAAAT ATCTGCAGGA TAAATCTGAA CAAAAACTAT CGATGACAAA GGAGCCAAGG 
AACAACCAAC CAATGTCATT TGATTCTTCA GAGGAGGTCA AAGTGTGCAC TGTTTTGGCT 
GGAAAGACCA ATAACTGTCA AACACATTTT ATTGCATCAC TGCGAGCCCG AATTGAGCAT 
CTGACGGAAG TTCGTTCAGT AGACGAGAGT GACATTGTTT TAATTTTCTG TCCAATTGTT 
TCTCGAGCTG GAACTGACAT TGATGCAGCC ATTCACAGAT GCTATACAAA TGCCTCTAAG 
CTGACTGTTC TAGTGGTGCT TCACCACACG TTTGACCCAG AGAAAACTGT ATCAGACAGC 
AGCAAAATTG TCAACAGGGA GAATCTGCTC ACAGTGGACT GTCTGTTCTA TGAGGATGCA 
GGATTACTGC AGTGTCAGAG GAATTCAGAT GCATATGACA AATCTGTCAA CTGGTTAATA 
GCGCAGGGCA AAAAAACAGG TCTTAAAATC TACCCAAAGA AATCACCTGA TAGTCAATCC 
AATGGTCGGA AATTCTGGAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100148510 ( XM_021476452.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332127.1 Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa142779
Clone ID Related Accession (Same CDS sequence)	XM_021476452.1 , XM_021471584.1
Accession Version	XM_021476452.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC100148510 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAGTA TGGACTTGGG AGTGGGAGTT AACATTGTTG AAACAGCAGC TCTGGGGAGA 
CCCTTCAGGC TGGGGATGCT GTACGACTGC AGAAAAGATG CATTTGTACC AGGAATCACA 
CTGTGGGATG AAGAGCAACT GCAGCAGAGC ATAAAAATCC ATCCTCAAAT TAAAACAGAT 
TTCTGTGTTA CAAATTCAGA CTTTATTGAG GCAAAATGCC AATTACTGAA CATTAATGAT 
TGTCTAAAAC TGAGTTTTTT AAGTGGACTC ATCAATGTTA GTGGAGCAGC CAAGTATCTC 
AGTGACACCA AGAAATCCTT CAAACAGCAG AGACTGACCC TACATTATCA TTCAACCACC 
AAACGTGAAG AACTGACTGT GAACCACTTG CCATCTGAAA ATATAACCCA CTATGAAGTG 
TTTGAGAATG ATGCAGCGAC ACATGTGGTG ACAGCTGTGC TGTATGGGGC CAATGCTTGC 
TTTGTTTTTG ACAGAGAAGC TTCATTAGTT GAAGACCAAA GCACTGTAGA CAGAGACGTA 
AAGGCTGCCT TAGAAAAACT AAAAGGGATT TCAGAAAGTA CACAGATTGA TCTGAGCTTG 
GATGACAATC AAAAGACTGT TTTTGAGAAA TTCAGCTGCA CATTTTATGG TGATTTCCAG 
TTAAAATCTA ATCCAGCCTC TTTTGAAGAA GCTCTGAGGG TTTTTGCTGA TCTTCCTAAA 
CTGCTGGGAG AAACCAAGGA GTGTGCAGTT CCACTGAGAG TGTGGCTTTA TCCACTGGAC 
AAACTGCATT CACATTATGT GAAACTTCAA CAAGAAATCA GCATAGATCT AATCAGAAAT 
GTTGAATCAG TATTTGAGAG CTTAAGGACC ACTGAAATGA AATGCAGTGA TCTTCTGAAT 
GATACACCAT CTTTGGCTTT TGCAGGATTT CATGATAAAA TAATGATTAT GAAGCAAAAC 
TGCTGCAACT ATAAGCTGAG CTTCATGAAG AAACTTGGAT CGCTATTGCC AAAAATCCGT 
AGTGATACAG AAAATGAGAC AGCCCTGATT GAGCTCCTCC ATGATCATGA AAAATGTCCA 
TTCAGAAAAA GGGATCTTGA GAAATGGGTG AAAGAAAAAG AGAATGAATC CGTCATTATT 
AAAACTCTTC TCAGACAATT GACTGATTCT GGAGCAACAG TGGAAAATAA TCTAGATGGA 
ATTTTAATGG ATCTTAATGT TGAAAATGTA GTAAGCTACA CTTTCACATC ACTTGAGTGT 
CCAGATGTGC TCCTTACTAA ACAAAAGGCT TTCCTGAATC CATCAACAAT AGAAAACAAC 
AGTGAGGATG CTTCTGTTTT AAGTAAAAAA TCTGGGTTCA CATCTGACAT CAAAACCACA 
ATGAGGAGCA ACTTGATCAT CTTCAAAAAC TTGATGGAAT CAAAAATATG TAAACCAGCC 
AAATATATTG TTGCATCGAA GGAAATACCA AATCCAGGTT CCTGCATTCT CATTTACGAA 
AATGGATCTG ATGAAGCGAC ATGCTTTACT CCGCCAATAA AACCAGCTCA TCCAGTCACT 
GAACAGATCA GTGGGGAAAG AGTGGTTTTA AAGGTGACAC CTGTGTGTCC TGCAACAGAA 
GAGCTCAGGT TACTGTACAA AACAAAAGAA GAGAAAGACT GGAAATCTCA GTCTGTGAAA 
AAGAGTCAAG ATACAGTTAT TCTGACTGAT CTCAGACCAG ACACTGAGTA TGAGATGAAA 
TTTGCAGCCG TGGGGAAGCT GAACTACACC ATAGACAGTG ATGTGATTCA CCTCAGAGTT 
ATTGACAAAA ACCTCACTGA TGCCACAGAG TCCATTTTAG AAAAGCTGAG CTTGATAGAA 
GACAAGTGCA GTAAACTGAT GGAGGGCAAG AGTGCAGTCA CATTTAGAGC AATTCGCAAC 
AAGTTTGAAG ACATGAAGAG ACACTGTCAG ACTTACAAAC AAGACAGAAT TAAATCCACA 
ATCCAGTCAG TTCAAGCCTG TGAAAAAGAC ATCAGTGCTC TGACAGACCT TCTACAAGCT 
CATCATGAGT CTCCATTTAA CATAAGTGAT ATTGTGGAGT GGATCAGAGT GAAAGAAACC 
GAATCAAAAG CAGTTGAGAA GATTCTTCAA CAGCTGCTGG AAACTGGAGC TGAAGTGAAC 
AATAGTTTAG ATGAATATTT GTTGGATGTT AGTTTTGAGA ATGTGGTGTG TTACACATTC 
ACTTCTCTTG ATCTGCCAGA CGACCTGCTT TCTGATCAAG AAGATTTCCT GAAACCTGAA 
ATGATAAGGA GAAATTCTGA TAAGAAACCC AATGCTGTGT CTGAAACTTG GCTCACAGGA 
AGTACCAGAG AGAAATTGAA AAAACAATTA GAAATGTTTA AAGAGCTCAA ATGTTCATAT 
GGCAGTCACT CAACAAAGTT TTTGGTTATA TCAGAAGATC AGACAAACCA TCCAGGTTCC 
TGTATTCTCC TTTATGAAAA TGGATCTTAT AAAGCGAAAT GCTTTACTCC GCCAATAAAA 
CCAGCTGAGC CCATCATTGA ACAGGTCATC GGGGAAAGAG TGGTTTTGAA GGTGACATCT 
GTGTGTCCTG CAACAGAAGA GCTCAGGTTA CTGTACAAAA CAAAAGAAGA GAACGACTGG 
AAATCTCAGT CTGTGAAAAA GAGTCAAGAT ACAGTTATTC TGACTGATCT CAGACCAGAC 
ACTGAGTATG AGATGAAATT TGCAGCTGTG GGGAAGCTGA ACTACACCAT AGACAGTGAT 
GTGATTCACC TTAGAGTTAT TGACAAGAAA CTCACTGATG CCACAGAGTC CATTTTAGAA 
AAGCTGAGCT TGATAGAAGA CAAGTGCAGT AAACTGATGG AGGACAAGAG TGCAGTCACT 
TTTAGAGCAA TTCACAACAA GTTTGAAGAC ATGAGAAGAC ACTGTCAGAT CTACAAACAA 
GACTTAGAAA TTAAAATTAC ATCCACAATG CAGTCAGTTC AAACCTGTGA AAAAGACATC 
AGTGCTCTGA CAGACCTTCT ACAAGCTCAT CATGAGTCTC CATTTAATGA AAGTGATCTT 
ATAGAGTGGA TCAGAGTGAA AGAAACCGAA TCAAATGCAG TTGAGAAGAT TCTTCAACAG 
CTGCTGGACT CTGGAACTGA GGTGAACAAT AGTTTAGATG AATATTTGTT TGATGTTAGT 
TTTGAGAATG TGGTGTGTTA CACATTCACT TCTCTTGATC TGCCAGACAA CCTGCTTTCT 
AAACAAGGAG ATTTCCTGAA ACCTGAAATA ATGAGGAGAA ATTCTAATAA GAACCCCAAT 
TCTGTGTCTG AAACTTGGCT CACAGGAAGT ATCAGAGAGA CTATGAAGAA GCATTTGGAA 
ATATTTAATG AGCTCAAATG TTCATATGGC AGTCACTCAT CAACATTTTT GGTTGTATAT 
AAAAGTCACA CAAACCATCC AGGTTCCTGC ATTCTTATTT ATGAAAATGG ATCTGATAAA 
GCGACATGCT TCACTCCTCC AATAAAACCA GCTAGTCCAG TCATTGAACA GATTGGTGAG 
GATATAGTGG TTTTGAAGGC AACACCATTA TGTCCTGCAA CACAAGAGCT CAGGTTACTG 
TACAAAAAAA AAGAAGAGAA TGATTGGAAA TCTCAGTCTG TGAAAACGAG TGGAGATGAA 
GTTTTTCTGA CTGGTCTCAG ACCTGACACT GAGTATAAGA TGAAATATGT GGCCTTGGGG 
AAGCTGAACT ACACCACAGA CAGTGATGTC ATCGGAGTTA CTACACGTGT GGATAAATCT 
GAACAAAAAC TATCGATGAC AAAGGAGCCA AGGAACAACC AACCAATGTC ATTTGATTCT 
TCAGAGGAGG TCAAAGTGTG CACTGTTTTG GCTGGAAAGA CCAATAACTG TCAAACACAT 
TTTATTGCAT CACTGCGAGC CCGAATTGAG CATCTGACGG AAGTTCGTTC AGTAGACGAG 
AGTGACATTG TTTTAATTTT CTGTCCAATT GTTTCTCGAG CTGGAACTGA CATTGATGCA 
GCCATTCACA GATGCTATAC AAATGCCTCT AAGCTGACTG TTCTAGTGGT GCTTCACCAC 
ACGTTTGACC CAGAGAAAAC TGTATCAGAC AGCAGCAAAA TTGTCAACAG GGAGAATCTG 
CTCACAGTGG ACTGTCTGTT CTATGAGGAT GCAGGATTAC TGCAGTGTCA GAGGAATTCA 
GATGCATATG ACAAATCTGT CAACTGGTTA ATAGCGCAGG GCAAAAAAAC AGGTCTTAAA 
ATCTACCCAA AGAAATCACC TGATAGTCAA TCCAATGGTC GGAAATTCTG GAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332127.1
CDS	301..4617
Translation	MRSMDLGVGVNIVETAALGRPFRLGMLYDCRKDAFVPGITLWDEEQLQQSIKIHPQIKTDFCVTNSDFIEAKCQLLNINDCLKLSFLSGLINVSGAAKYLSDTKKSFKQQRLTLHYHSTTKREELTVNHLPSENITHYEVFENDAATHVVTAVLYGANACFVFDREASLVEDQSTVDRDVKAALEKLKGISESTQIDLSLDDNQKTVFEKFSCTFYGDFQLKSNPASFEEALRVFADLPKLLGETKECAVPLRVWLYPLDKLHSHYVKLQQEISIDLIRNVESVFESLRTTEMKCSDLLNDTPSLAFAGFHDKIMIMKQNCCNYKLSFMKKLGSLLPKIRSDTENETALIELLHDHEKCPFRKRDLEKWVKEKENESVIIKTLLRQLTDSGATVENNLDGILMDLNVENVVSYTFTSLECPDVLLTKQKAFLNPSTIENNSEDASVLSKKSGFTSDIKTTMRSNLIIFKNLMESKICKPAKYIVASKEIPNPGSCILIYENGSDEATCFTPPIKPAHPVTEQISGERVVLKVTPVCPATEELRLLYKTKEEKDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKNLTDATESILEKLSLIEDKCSKLMEGKSAVTFRAIRNKFEDMKRHCQTYKQDRIKSTIQSVQACEKDISALTDLLQAHHESPFNISDIVEWIRVKETESKAVEKILQQLLETGAEVNNSLDEYLLDVSFENVVCYTFTSLDLPDDLLSDQEDFLKPEMIRRNSDKKPNAVSETWLTGSTREKLKKQLEMFKELKCSYGSHSTKFLVISEDQTNHPGSCILLYENGSYKAKCFTPPIKPAEPIIEQVIGERVVLKVTSVCPATEELRLLYKTKEENDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKKLTDATESILEKLSLIEDKCSKLMEDKSAVTFRAIHNKFEDMRRHCQIYKQDLEIKITSTMQSVQTCEKDISALTDLLQAHHESPFNESDLIEWIRVKETESNAVEKILQQLLDSGTEVNNSLDEYLFDVSFENVVCYTFTSLDLPDNLLSKQGDFLKPEIMRRNSNKNPNSVSETWLTGSIRETMKKHLEIFNELKCSYGSHSSTFLVVYKSHTNHPGSCILIYENGSDKATCFTPPIKPASPVIEQIGEDIVVLKATPLCPATQELRLLYKKKEENDWKSQSVKTSGDEVFLTGLRPDTEYKMKYVALGKLNYTTDSDVIGVTTRVDKSEQKLSMTKEPRNNQPMSFDSSEEVKVCTVLAGKTNNCQTHFIASLRARIEHLTEVRSVDESDIVLIFCPIVSRAGTDIDAAIHRCYTNASKLTVLVVLHHTFDPEKTVSDSSKIVNRENLLTVDCLFYEDAGLLQCQRNSDAYDKSVNWLIAQGKKTGLKIYPKKSPDSQSNGRKFWR

Target ORF information:

RefSeq Version	XM_021476452.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476452.1

ORF Insert Sequence:

ATGCGCAGTA TGGACTTGGG AGTGGGAGTT AACATTGTTG AAACAGCAGC TCTGGGGAGA 
CCCTTCAGGC TGGGGATGCT GTACGACTGC AGAAAAGATG CATTTGTACC AGGAATCACA 
CTGTGGGATG AAGAGCAACT GCAGCAGAGC ATAAAAATCC ATCCTCAAAT TAAAACAGAT 
TTCTGTGTTA CAAATTCAGA CTTTATTGAG GCAAAATGCC AATTACTGAA CATTAATGAT 
TGTCTAAAAC TGAGTTTTTT AAGTGGACTC ATCAATGTTA GTGGAGCAGC CAAGTATCTC 
AGTGACACCA AGAAATCCTT CAAACAGCAG AGACTGACCC TACATTATCA TTCAACCACC 
AAACGTGAAG AACTGACTGT GAACCACTTG CCATCTGAAA ATATAACCCA CTATGAAGTG 
TTTGAGAATG ATGCAGCGAC ACATGTGGTG ACAGCTGTGC TGTATGGGGC CAATGCTTGC 
TTTGTTTTTG ACAGAGAAGC TTCATTAGTT GAAGACCAAA GCACTGTAGA CAGAGACGTA 
AAGGCTGCCT TAGAAAAACT AAAAGGGATT TCAGAAAGTA CACAGATTGA TCTGAGCTTG 
GATGACAATC AAAAGACTGT TTTTGAGAAA TTCAGCTGCA CATTTTATGG TGATTTCCAG 
TTAAAATCTA ATCCAGCCTC TTTTGAAGAA GCTCTGAGGG TTTTTGCTGA TCTTCCTAAA 
CTGCTGGGAG AAACCAAGGA GTGTGCAGTT CCACTGAGAG TGTGGCTTTA TCCACTGGAC 
AAACTGCATT CACATTATGT GAAACTTCAA CAAGAAATCA GCATAGATCT AATCAGAAAT 
GTTGAATCAG TATTTGAGAG CTTAAGGACC ACTGAAATGA AATGCAGTGA TCTTCTGAAT 
GATACACCAT CTTTGGCTTT TGCAGGATTT CATGATAAAA TAATGATTAT GAAGCAAAAC 
TGCTGCAACT ATAAGCTGAG CTTCATGAAG AAACTTGGAT CGCTATTGCC AAAAATCCGT 
AGTGATACAG AAAATGAGAC AGCCCTGATT GAGCTCCTCC ATGATCATGA AAAATGTCCA 
TTCAGAAAAA GGGATCTTGA GAAATGGGTG AAAGAAAAAG AGAATGAATC CGTCATTATT 
AAAACTCTTC TCAGACAATT GACTGATTCT GGAGCAACAG TGGAAAATAA TCTAGATGGA 
ATTTTAATGG ATCTTAATGT TGAAAATGTA GTAAGCTACA CTTTCACATC ACTTGAGTGT 
CCAGATGTGC TCCTTACTAA ACAAAAGGCT TTCCTGAATC CATCAACAAT AGAAAACAAC 
AGTGAGGATG CTTCTGTTTT AAGTAAAAAA TCTGGGTTCA CATCTGACAT CAAAACCACA 
ATGAGGAGCA ACTTGATCAT CTTCAAAAAC TTGATGGAAT CAAAAATATG TAAACCAGCC 
AAATATATTG TTGCATCGAA GGAAATACCA AATCCAGGTT CCTGCATTCT CATTTACGAA 
AATGGATCTG ATGAAGCGAC ATGCTTTACT CCGCCAATAA AACCAGCTCA TCCAGTCACT 
GAACAGATCA GTGGGGAAAG AGTGGTTTTA AAGGTGACAC CTGTGTGTCC TGCAACAGAA 
GAGCTCAGGT TACTGTACAA AACAAAAGAA GAGAAAGACT GGAAATCTCA GTCTGTGAAA 
AAGAGTCAAG ATACAGTTAT TCTGACTGAT CTCAGACCAG ACACTGAGTA TGAGATGAAA 
TTTGCAGCCG TGGGGAAGCT GAACTACACC ATAGACAGTG ATGTGATTCA CCTCAGAGTT 
ATTGACAAAA ACCTCACTGA TGCCACAGAG TCCATTTTAG AAAAGCTGAG CTTGATAGAA 
GACAAGTGCA GTAAACTGAT GGAGGGCAAG AGTGCAGTCA CATTTAGAGC AATTCGCAAC 
AAGTTTGAAG ACATGAAGAG ACACTGTCAG ACTTACAAAC AAGACAGAAT TAAATCCACA 
ATCCAGTCAG TTCAAGCCTG TGAAAAAGAC ATCAGTGCTC TGACAGACCT TCTACAAGCT 
CATCATGAGT CTCCATTTAA CATAAGTGAT ATTGTGGAGT GGATCAGAGT GAAAGAAACC 
GAATCAAAAG CAGTTGAGAA GATTCTTCAA CAGCTGCTGG AAACTGGAGC TGAAGTGAAC 
AATAGTTTAG ATGAATATTT GTTGGATGTT AGTTTTGAGA ATGTGGTGTG TTACACATTC 
ACTTCTCTTG ATCTGCCAGA CGACCTGCTT TCTGATCAAG AAGATTTCCT GAAACCTGAA 
ATGATAAGGA GAAATTCTGA TAAGAAACCC AATGCTGTGT CTGAAACTTG GCTCACAGGA 
AGTACCAGAG AGAAATTGAA AAAACAATTA GAAATGTTTA AAGAGCTCAA ATGTTCATAT 
GGCAGTCACT CAACAAAGTT TTTGGTTATA TCAGAAGATC AGACAAACCA TCCAGGTTCC 
TGTATTCTCC TTTATGAAAA TGGATCTTAT AAAGCGAAAT GCTTTACTCC GCCAATAAAA 
CCAGCTGAGC CCATCATTGA ACAGGTCATC GGGGAAAGAG TGGTTTTGAA GGTGACATCT 
GTGTGTCCTG CAACAGAAGA GCTCAGGTTA CTGTACAAAA CAAAAGAAGA GAACGACTGG 
AAATCTCAGT CTGTGAAAAA GAGTCAAGAT ACAGTTATTC TGACTGATCT CAGACCAGAC 
ACTGAGTATG AGATGAAATT TGCAGCTGTG GGGAAGCTGA ACTACACCAT AGACAGTGAT 
GTGATTCACC TTAGAGTTAT TGACAAGAAA CTCACTGATG CCACAGAGTC CATTTTAGAA 
AAGCTGAGCT TGATAGAAGA CAAGTGCAGT AAACTGATGG AGGACAAGAG TGCAGTCACT 
TTTAGAGCAA TTCACAACAA GTTTGAAGAC ATGAGAAGAC ACTGTCAGAT CTACAAACAA 
GACTTAGAAA TTAAAATTAC ATCCACAATG CAGTCAGTTC AAACCTGTGA AAAAGACATC 
AGTGCTCTGA CAGACCTTCT ACAAGCTCAT CATGAGTCTC CATTTAATGA AAGTGATCTT 
ATAGAGTGGA TCAGAGTGAA AGAAACCGAA TCAAATGCAG TTGAGAAGAT TCTTCAACAG 
CTGCTGGACT CTGGAACTGA GGTGAACAAT AGTTTAGATG AATATTTGTT TGATGTTAGT 
TTTGAGAATG TGGTGTGTTA CACATTCACT TCTCTTGATC TGCCAGACAA CCTGCTTTCT 
AAACAAGGAG ATTTCCTGAA ACCTGAAATA ATGAGGAGAA ATTCTAATAA GAACCCCAAT 
TCTGTGTCTG AAACTTGGCT CACAGGAAGT ATCAGAGAGA CTATGAAGAA GCATTTGGAA 
ATATTTAATG AGCTCAAATG TTCATATGGC AGTCACTCAT CAACATTTTT GGTTGTATAT 
AAAAGTCACA CAAACCATCC AGGTTCCTGC ATTCTTATTT ATGAAAATGG ATCTGATAAA 
GCGACATGCT TCACTCCTCC AATAAAACCA GCTAGTCCAG TCATTGAACA GATTGGTGAG 
GATATAGTGG TTTTGAAGGC AACACCATTA TGTCCTGCAA CACAAGAGCT CAGGTTACTG 
TACAAAAAAA AAGAAGAGAA TGATTGGAAA TCTCAGTCTG TGAAAACGAG TGGAGATGAA 
GTTTTTCTGA CTGGTCTCAG ACCTGACACT GAGTATAAGA TGAAATATGT GGCCTTGGGG 
AAGCTGAACT ACACCACAGA CAGTGATGTC ATCGGAGTTA CTACACGTGT GGATAAATCT 
GAACAAAAAC TATCGATGAC AAAGGAGCCA AGGAACAACC AACCAATGTC ATTTGATTCT 
TCAGAGGAGG TCAAAGTGTG CACTGTTTTG GCTGGAAAGA CCAATAACTG TCAAACACAT 
TTTATTGCAT CACTGCGAGC CCGAATTGAG CATCTGACGG AAGTTCGTTC AGTAGACGAG 
AGTGACATTG TTTTAATTTT CTGTCCAATT GTTTCTCGAG CTGGAACTGA CATTGATGCA 
GCCATTCACA GATGCTATAC AAATGCCTCT AAGCTGACTG TTCTAGTGGT GCTTCACCAC 
ACGTTTGACC CAGAGAAAAC TGTATCAGAC AGCAGCAAAA TTGTCAACAG GGAGAATCTG 
CTCACAGTGG ACTGTCTGTT CTATGAGGAT GCAGGATTAC TGCAGTGTCA GAGGAATTCA 
GATGCATATG ACAAATCTGT CAACTGGTTA ATAGCGCAGG GCAAAAAAAC AGGTCTTAAA 
ATCTACCCAA AGAAATCACC TGATAGTCAA TCCAATGGTC GGAAATTCTG GAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100148510 ( XM_021471584.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327259.1 Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X5, mRNA.

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CloneID	ODa142779
Clone ID Related Accession (Same CDS sequence)	XM_021476452.1 , XM_021471584.1
Accession Version	XM_021471584.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC100148510 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAGTA TGGACTTGGG AGTGGGAGTT AACATTGTTG AAACAGCAGC TCTGGGGAGA 
CCCTTCAGGC TGGGGATGCT GTACGACTGC AGAAAAGATG CATTTGTACC AGGAATCACA 
CTGTGGGATG AAGAGCAACT GCAGCAGAGC ATAAAAATCC ATCCTCAAAT TAAAACAGAT 
TTCTGTGTTA CAAATTCAGA CTTTATTGAG GCAAAATGCC AATTACTGAA CATTAATGAT 
TGTCTAAAAC TGAGTTTTTT AAGTGGACTC ATCAATGTTA GTGGAGCAGC CAAGTATCTC 
AGTGACACCA AGAAATCCTT CAAACAGCAG AGACTGACCC TACATTATCA TTCAACCACC 
AAACGTGAAG AACTGACTGT GAACCACTTG CCATCTGAAA ATATAACCCA CTATGAAGTG 
TTTGAGAATG ATGCAGCGAC ACATGTGGTG ACAGCTGTGC TGTATGGGGC CAATGCTTGC 
TTTGTTTTTG ACAGAGAAGC TTCATTAGTT GAAGACCAAA GCACTGTAGA CAGAGACGTA 
AAGGCTGCCT TAGAAAAACT AAAAGGGATT TCAGAAAGTA CACAGATTGA TCTGAGCTTG 
GATGACAATC AAAAGACTGT TTTTGAGAAA TTCAGCTGCA CATTTTATGG TGATTTCCAG 
TTAAAATCTA ATCCAGCCTC TTTTGAAGAA GCTCTGAGGG TTTTTGCTGA TCTTCCTAAA 
CTGCTGGGAG AAACCAAGGA GTGTGCAGTT CCACTGAGAG TGTGGCTTTA TCCACTGGAC 
AAACTGCATT CACATTATGT GAAACTTCAA CAAGAAATCA GCATAGATCT AATCAGAAAT 
GTTGAATCAG TATTTGAGAG CTTAAGGACC ACTGAAATGA AATGCAGTGA TCTTCTGAAT 
GATACACCAT CTTTGGCTTT TGCAGGATTT CATGATAAAA TAATGATTAT GAAGCAAAAC 
TGCTGCAACT ATAAGCTGAG CTTCATGAAG AAACTTGGAT CGCTATTGCC AAAAATCCGT 
AGTGATACAG AAAATGAGAC AGCCCTGATT GAGCTCCTCC ATGATCATGA AAAATGTCCA 
TTCAGAAAAA GGGATCTTGA GAAATGGGTG AAAGAAAAAG AGAATGAATC CGTCATTATT 
AAAACTCTTC TCAGACAATT GACTGATTCT GGAGCAACAG TGGAAAATAA TCTAGATGGA 
ATTTTAATGG ATCTTAATGT TGAAAATGTA GTAAGCTACA CTTTCACATC ACTTGAGTGT 
CCAGATGTGC TCCTTACTAA ACAAAAGGCT TTCCTGAATC CATCAACAAT AGAAAACAAC 
AGTGAGGATG CTTCTGTTTT AAGTAAAAAA TCTGGGTTCA CATCTGACAT CAAAACCACA 
ATGAGGAGCA ACTTGATCAT CTTCAAAAAC TTGATGGAAT CAAAAATATG TAAACCAGCC 
AAATATATTG TTGCATCGAA GGAAATACCA AATCCAGGTT CCTGCATTCT CATTTACGAA 
AATGGATCTG ATGAAGCGAC ATGCTTTACT CCGCCAATAA AACCAGCTCA TCCAGTCACT 
GAACAGATCA GTGGGGAAAG AGTGGTTTTA AAGGTGACAC CTGTGTGTCC TGCAACAGAA 
GAGCTCAGGT TACTGTACAA AACAAAAGAA GAGAAAGACT GGAAATCTCA GTCTGTGAAA 
AAGAGTCAAG ATACAGTTAT TCTGACTGAT CTCAGACCAG ACACTGAGTA TGAGATGAAA 
TTTGCAGCCG TGGGGAAGCT GAACTACACC ATAGACAGTG ATGTGATTCA CCTCAGAGTT 
ATTGACAAAA ACCTCACTGA TGCCACAGAG TCCATTTTAG AAAAGCTGAG CTTGATAGAA 
GACAAGTGCA GTAAACTGAT GGAGGGCAAG AGTGCAGTCA CATTTAGAGC AATTCGCAAC 
AAGTTTGAAG ACATGAAGAG ACACTGTCAG ACTTACAAAC AAGACAGAAT TAAATCCACA 
ATCCAGTCAG TTCAAGCCTG TGAAAAAGAC ATCAGTGCTC TGACAGACCT TCTACAAGCT 
CATCATGAGT CTCCATTTAA CATAAGTGAT ATTGTGGAGT GGATCAGAGT GAAAGAAACC 
GAATCAAAAG CAGTTGAGAA GATTCTTCAA CAGCTGCTGG AAACTGGAGC TGAAGTGAAC 
AATAGTTTAG ATGAATATTT GTTGGATGTT AGTTTTGAGA ATGTGGTGTG TTACACATTC 
ACTTCTCTTG ATCTGCCAGA CGACCTGCTT TCTGATCAAG AAGATTTCCT GAAACCTGAA 
ATGATAAGGA GAAATTCTGA TAAGAAACCC AATGCTGTGT CTGAAACTTG GCTCACAGGA 
AGTACCAGAG AGAAATTGAA AAAACAATTA GAAATGTTTA AAGAGCTCAA ATGTTCATAT 
GGCAGTCACT CAACAAAGTT TTTGGTTATA TCAGAAGATC AGACAAACCA TCCAGGTTCC 
TGTATTCTCC TTTATGAAAA TGGATCTTAT AAAGCGAAAT GCTTTACTCC GCCAATAAAA 
CCAGCTGAGC CCATCATTGA ACAGGTCATC GGGGAAAGAG TGGTTTTGAA GGTGACATCT 
GTGTGTCCTG CAACAGAAGA GCTCAGGTTA CTGTACAAAA CAAAAGAAGA GAACGACTGG 
AAATCTCAGT CTGTGAAAAA GAGTCAAGAT ACAGTTATTC TGACTGATCT CAGACCAGAC 
ACTGAGTATG AGATGAAATT TGCAGCTGTG GGGAAGCTGA ACTACACCAT AGACAGTGAT 
GTGATTCACC TTAGAGTTAT TGACAAGAAA CTCACTGATG CCACAGAGTC CATTTTAGAA 
AAGCTGAGCT TGATAGAAGA CAAGTGCAGT AAACTGATGG AGGACAAGAG TGCAGTCACT 
TTTAGAGCAA TTCACAACAA GTTTGAAGAC ATGAGAAGAC ACTGTCAGAT CTACAAACAA 
GACTTAGAAA TTAAAATTAC ATCCACAATG CAGTCAGTTC AAACCTGTGA AAAAGACATC 
AGTGCTCTGA CAGACCTTCT ACAAGCTCAT CATGAGTCTC CATTTAATGA AAGTGATCTT 
ATAGAGTGGA TCAGAGTGAA AGAAACCGAA TCAAATGCAG TTGAGAAGAT TCTTCAACAG 
CTGCTGGACT CTGGAACTGA GGTGAACAAT AGTTTAGATG AATATTTGTT TGATGTTAGT 
TTTGAGAATG TGGTGTGTTA CACATTCACT TCTCTTGATC TGCCAGACAA CCTGCTTTCT 
AAACAAGGAG ATTTCCTGAA ACCTGAAATA ATGAGGAGAA ATTCTAATAA GAACCCCAAT 
TCTGTGTCTG AAACTTGGCT CACAGGAAGT ATCAGAGAGA CTATGAAGAA GCATTTGGAA 
ATATTTAATG AGCTCAAATG TTCATATGGC AGTCACTCAT CAACATTTTT GGTTGTATAT 
AAAAGTCACA CAAACCATCC AGGTTCCTGC ATTCTTATTT ATGAAAATGG ATCTGATAAA 
GCGACATGCT TCACTCCTCC AATAAAACCA GCTAGTCCAG TCATTGAACA GATTGGTGAG 
GATATAGTGG TTTTGAAGGC AACACCATTA TGTCCTGCAA CACAAGAGCT CAGGTTACTG 
TACAAAAAAA AAGAAGAGAA TGATTGGAAA TCTCAGTCTG TGAAAACGAG TGGAGATGAA 
GTTTTTCTGA CTGGTCTCAG ACCTGACACT GAGTATAAGA TGAAATATGT GGCCTTGGGG 
AAGCTGAACT ACACCACAGA CAGTGATGTC ATCGGAGTTA CTACACGTGT GGATAAATCT 
GAACAAAAAC TATCGATGAC AAAGGAGCCA AGGAACAACC AACCAATGTC ATTTGATTCT 
TCAGAGGAGG TCAAAGTGTG CACTGTTTTG GCTGGAAAGA CCAATAACTG TCAAACACAT 
TTTATTGCAT CACTGCGAGC CCGAATTGAG CATCTGACGG AAGTTCGTTC AGTAGACGAG 
AGTGACATTG TTTTAATTTT CTGTCCAATT GTTTCTCGAG CTGGAACTGA CATTGATGCA 
GCCATTCACA GATGCTATAC AAATGCCTCT AAGCTGACTG TTCTAGTGGT GCTTCACCAC 
ACGTTTGACC CAGAGAAAAC TGTATCAGAC AGCAGCAAAA TTGTCAACAG GGAGAATCTG 
CTCACAGTGG ACTGTCTGTT CTATGAGGAT GCAGGATTAC TGCAGTGTCA GAGGAATTCA 
GATGCATATG ACAAATCTGT CAACTGGTTA ATAGCGCAGG GCAAAAAAAC AGGTCTTAAA 
ATCTACCCAA AGAAATCACC TGATAGTCAA TCCAATGGTC GGAAATTCTG GAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327259.1
CDS	301..4617
Translation	MRSMDLGVGVNIVETAALGRPFRLGMLYDCRKDAFVPGITLWDEEQLQQSIKIHPQIKTDFCVTNSDFIEAKCQLLNINDCLKLSFLSGLINVSGAAKYLSDTKKSFKQQRLTLHYHSTTKREELTVNHLPSENITHYEVFENDAATHVVTAVLYGANACFVFDREASLVEDQSTVDRDVKAALEKLKGISESTQIDLSLDDNQKTVFEKFSCTFYGDFQLKSNPASFEEALRVFADLPKLLGETKECAVPLRVWLYPLDKLHSHYVKLQQEISIDLIRNVESVFESLRTTEMKCSDLLNDTPSLAFAGFHDKIMIMKQNCCNYKLSFMKKLGSLLPKIRSDTENETALIELLHDHEKCPFRKRDLEKWVKEKENESVIIKTLLRQLTDSGATVENNLDGILMDLNVENVVSYTFTSLECPDVLLTKQKAFLNPSTIENNSEDASVLSKKSGFTSDIKTTMRSNLIIFKNLMESKICKPAKYIVASKEIPNPGSCILIYENGSDEATCFTPPIKPAHPVTEQISGERVVLKVTPVCPATEELRLLYKTKEEKDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKNLTDATESILEKLSLIEDKCSKLMEGKSAVTFRAIRNKFEDMKRHCQTYKQDRIKSTIQSVQACEKDISALTDLLQAHHESPFNISDIVEWIRVKETESKAVEKILQQLLETGAEVNNSLDEYLLDVSFENVVCYTFTSLDLPDDLLSDQEDFLKPEMIRRNSDKKPNAVSETWLTGSTREKLKKQLEMFKELKCSYGSHSTKFLVISEDQTNHPGSCILLYENGSYKAKCFTPPIKPAEPIIEQVIGERVVLKVTSVCPATEELRLLYKTKEENDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKKLTDATESILEKLSLIEDKCSKLMEDKSAVTFRAIHNKFEDMRRHCQIYKQDLEIKITSTMQSVQTCEKDISALTDLLQAHHESPFNESDLIEWIRVKETESNAVEKILQQLLDSGTEVNNSLDEYLFDVSFENVVCYTFTSLDLPDNLLSKQGDFLKPEIMRRNSNKNPNSVSETWLTGSIRETMKKHLEIFNELKCSYGSHSSTFLVVYKSHTNHPGSCILIYENGSDKATCFTPPIKPASPVIEQIGEDIVVLKATPLCPATQELRLLYKKKEENDWKSQSVKTSGDEVFLTGLRPDTEYKMKYVALGKLNYTTDSDVIGVTTRVDKSEQKLSMTKEPRNNQPMSFDSSEEVKVCTVLAGKTNNCQTHFIASLRARIEHLTEVRSVDESDIVLIFCPIVSRAGTDIDAAIHRCYTNASKLTVLVVLHHTFDPEKTVSDSSKIVNRENLLTVDCLFYEDAGLLQCQRNSDAYDKSVNWLIAQGKKTGLKIYPKKSPDSQSNGRKFWR

Target ORF information:

RefSeq Version	XM_021471584.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471584.1

ORF Insert Sequence:

ATGCGCAGTA TGGACTTGGG AGTGGGAGTT AACATTGTTG AAACAGCAGC TCTGGGGAGA 
CCCTTCAGGC TGGGGATGCT GTACGACTGC AGAAAAGATG CATTTGTACC AGGAATCACA 
CTGTGGGATG AAGAGCAACT GCAGCAGAGC ATAAAAATCC ATCCTCAAAT TAAAACAGAT 
TTCTGTGTTA CAAATTCAGA CTTTATTGAG GCAAAATGCC AATTACTGAA CATTAATGAT 
TGTCTAAAAC TGAGTTTTTT AAGTGGACTC ATCAATGTTA GTGGAGCAGC CAAGTATCTC 
AGTGACACCA AGAAATCCTT CAAACAGCAG AGACTGACCC TACATTATCA TTCAACCACC 
AAACGTGAAG AACTGACTGT GAACCACTTG CCATCTGAAA ATATAACCCA CTATGAAGTG 
TTTGAGAATG ATGCAGCGAC ACATGTGGTG ACAGCTGTGC TGTATGGGGC CAATGCTTGC 
TTTGTTTTTG ACAGAGAAGC TTCATTAGTT GAAGACCAAA GCACTGTAGA CAGAGACGTA 
AAGGCTGCCT TAGAAAAACT AAAAGGGATT TCAGAAAGTA CACAGATTGA TCTGAGCTTG 
GATGACAATC AAAAGACTGT TTTTGAGAAA TTCAGCTGCA CATTTTATGG TGATTTCCAG 
TTAAAATCTA ATCCAGCCTC TTTTGAAGAA GCTCTGAGGG TTTTTGCTGA TCTTCCTAAA 
CTGCTGGGAG AAACCAAGGA GTGTGCAGTT CCACTGAGAG TGTGGCTTTA TCCACTGGAC 
AAACTGCATT CACATTATGT GAAACTTCAA CAAGAAATCA GCATAGATCT AATCAGAAAT 
GTTGAATCAG TATTTGAGAG CTTAAGGACC ACTGAAATGA AATGCAGTGA TCTTCTGAAT 
GATACACCAT CTTTGGCTTT TGCAGGATTT CATGATAAAA TAATGATTAT GAAGCAAAAC 
TGCTGCAACT ATAAGCTGAG CTTCATGAAG AAACTTGGAT CGCTATTGCC AAAAATCCGT 
AGTGATACAG AAAATGAGAC AGCCCTGATT GAGCTCCTCC ATGATCATGA AAAATGTCCA 
TTCAGAAAAA GGGATCTTGA GAAATGGGTG AAAGAAAAAG AGAATGAATC CGTCATTATT 
AAAACTCTTC TCAGACAATT GACTGATTCT GGAGCAACAG TGGAAAATAA TCTAGATGGA 
ATTTTAATGG ATCTTAATGT TGAAAATGTA GTAAGCTACA CTTTCACATC ACTTGAGTGT 
CCAGATGTGC TCCTTACTAA ACAAAAGGCT TTCCTGAATC CATCAACAAT AGAAAACAAC 
AGTGAGGATG CTTCTGTTTT AAGTAAAAAA TCTGGGTTCA CATCTGACAT CAAAACCACA 
ATGAGGAGCA ACTTGATCAT CTTCAAAAAC TTGATGGAAT CAAAAATATG TAAACCAGCC 
AAATATATTG TTGCATCGAA GGAAATACCA AATCCAGGTT CCTGCATTCT CATTTACGAA 
AATGGATCTG ATGAAGCGAC ATGCTTTACT CCGCCAATAA AACCAGCTCA TCCAGTCACT 
GAACAGATCA GTGGGGAAAG AGTGGTTTTA AAGGTGACAC CTGTGTGTCC TGCAACAGAA 
GAGCTCAGGT TACTGTACAA AACAAAAGAA GAGAAAGACT GGAAATCTCA GTCTGTGAAA 
AAGAGTCAAG ATACAGTTAT TCTGACTGAT CTCAGACCAG ACACTGAGTA TGAGATGAAA 
TTTGCAGCCG TGGGGAAGCT GAACTACACC ATAGACAGTG ATGTGATTCA CCTCAGAGTT 
ATTGACAAAA ACCTCACTGA TGCCACAGAG TCCATTTTAG AAAAGCTGAG CTTGATAGAA 
GACAAGTGCA GTAAACTGAT GGAGGGCAAG AGTGCAGTCA CATTTAGAGC AATTCGCAAC 
AAGTTTGAAG ACATGAAGAG ACACTGTCAG ACTTACAAAC AAGACAGAAT TAAATCCACA 
ATCCAGTCAG TTCAAGCCTG TGAAAAAGAC ATCAGTGCTC TGACAGACCT TCTACAAGCT 
CATCATGAGT CTCCATTTAA CATAAGTGAT ATTGTGGAGT GGATCAGAGT GAAAGAAACC 
GAATCAAAAG CAGTTGAGAA GATTCTTCAA CAGCTGCTGG AAACTGGAGC TGAAGTGAAC 
AATAGTTTAG ATGAATATTT GTTGGATGTT AGTTTTGAGA ATGTGGTGTG TTACACATTC 
ACTTCTCTTG ATCTGCCAGA CGACCTGCTT TCTGATCAAG AAGATTTCCT GAAACCTGAA 
ATGATAAGGA GAAATTCTGA TAAGAAACCC AATGCTGTGT CTGAAACTTG GCTCACAGGA 
AGTACCAGAG AGAAATTGAA AAAACAATTA GAAATGTTTA AAGAGCTCAA ATGTTCATAT 
GGCAGTCACT CAACAAAGTT TTTGGTTATA TCAGAAGATC AGACAAACCA TCCAGGTTCC 
TGTATTCTCC TTTATGAAAA TGGATCTTAT AAAGCGAAAT GCTTTACTCC GCCAATAAAA 
CCAGCTGAGC CCATCATTGA ACAGGTCATC GGGGAAAGAG TGGTTTTGAA GGTGACATCT 
GTGTGTCCTG CAACAGAAGA GCTCAGGTTA CTGTACAAAA CAAAAGAAGA GAACGACTGG 
AAATCTCAGT CTGTGAAAAA GAGTCAAGAT ACAGTTATTC TGACTGATCT CAGACCAGAC 
ACTGAGTATG AGATGAAATT TGCAGCTGTG GGGAAGCTGA ACTACACCAT AGACAGTGAT 
GTGATTCACC TTAGAGTTAT TGACAAGAAA CTCACTGATG CCACAGAGTC CATTTTAGAA 
AAGCTGAGCT TGATAGAAGA CAAGTGCAGT AAACTGATGG AGGACAAGAG TGCAGTCACT 
TTTAGAGCAA TTCACAACAA GTTTGAAGAC ATGAGAAGAC ACTGTCAGAT CTACAAACAA 
GACTTAGAAA TTAAAATTAC ATCCACAATG CAGTCAGTTC AAACCTGTGA AAAAGACATC 
AGTGCTCTGA CAGACCTTCT ACAAGCTCAT CATGAGTCTC CATTTAATGA AAGTGATCTT 
ATAGAGTGGA TCAGAGTGAA AGAAACCGAA TCAAATGCAG TTGAGAAGAT TCTTCAACAG 
CTGCTGGACT CTGGAACTGA GGTGAACAAT AGTTTAGATG AATATTTGTT TGATGTTAGT 
TTTGAGAATG TGGTGTGTTA CACATTCACT TCTCTTGATC TGCCAGACAA CCTGCTTTCT 
AAACAAGGAG ATTTCCTGAA ACCTGAAATA ATGAGGAGAA ATTCTAATAA GAACCCCAAT 
TCTGTGTCTG AAACTTGGCT CACAGGAAGT ATCAGAGAGA CTATGAAGAA GCATTTGGAA 
ATATTTAATG AGCTCAAATG TTCATATGGC AGTCACTCAT CAACATTTTT GGTTGTATAT 
AAAAGTCACA CAAACCATCC AGGTTCCTGC ATTCTTATTT ATGAAAATGG ATCTGATAAA 
GCGACATGCT TCACTCCTCC AATAAAACCA GCTAGTCCAG TCATTGAACA GATTGGTGAG 
GATATAGTGG TTTTGAAGGC AACACCATTA TGTCCTGCAA CACAAGAGCT CAGGTTACTG 
TACAAAAAAA AAGAAGAGAA TGATTGGAAA TCTCAGTCTG TGAAAACGAG TGGAGATGAA 
GTTTTTCTGA CTGGTCTCAG ACCTGACACT GAGTATAAGA TGAAATATGT GGCCTTGGGG 
AAGCTGAACT ACACCACAGA CAGTGATGTC ATCGGAGTTA CTACACGTGT GGATAAATCT 
GAACAAAAAC TATCGATGAC AAAGGAGCCA AGGAACAACC AACCAATGTC ATTTGATTCT 
TCAGAGGAGG TCAAAGTGTG CACTGTTTTG GCTGGAAAGA CCAATAACTG TCAAACACAT 
TTTATTGCAT CACTGCGAGC CCGAATTGAG CATCTGACGG AAGTTCGTTC AGTAGACGAG 
AGTGACATTG TTTTAATTTT CTGTCCAATT GTTTCTCGAG CTGGAACTGA CATTGATGCA 
GCCATTCACA GATGCTATAC AAATGCCTCT AAGCTGACTG TTCTAGTGGT GCTTCACCAC 
ACGTTTGACC CAGAGAAAAC TGTATCAGAC AGCAGCAAAA TTGTCAACAG GGAGAATCTG 
CTCACAGTGG ACTGTCTGTT CTATGAGGAT GCAGGATTAC TGCAGTGTCA GAGGAATTCA 
GATGCATATG ACAAATCTGT CAACTGGTTA ATAGCGCAGG GCAAAAAAAC AGGTCTTAAA 
ATCTACCCAA AGAAATCACC TGATAGTCAA TCCAATGGTC GGAAATTCTG GAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100148510 ( XM_009301399.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299674.1 Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X2, mRNA.

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CloneID	ODa48648
Clone ID Related Accession (Same CDS sequence)	XM_009301399.2
Accession Version	XM_009301399.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein LOC100148510 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301399.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCATGC ACAACTCAAA AATAAGCAAA TATGACGCAT CACAATGTAT GAAAAAACAA 
CAATTAAAAA AAATCTTGCC ATCGCCACTT ATAAATGCTT GGATTTACGT GCTTGAGGTT 
ACCATAGAAA TTATGGACTT GGGAGTGGGA GTTAACATTG TTGAAACAGC AGCTCTGGGG 
AGACCCTTCA GGCTGGGGAT GCTGTACGAC TGCAGAAAAG ATGCATTTGT ACCAGGAATC 
ACACTGTGGG ATGAAGAGCA ACTGCAGCAG AGCATAAAAA TCCATCCTCA AATTAAAACA 
GATTTCTGTG TTACAAATTC AGACTTTATT GAGGCAAAAT GCCAATTACT GAACATTAAT 
GATTGTCTAA AACTGAGTTT TTTAAGTGGA CTCATCAATG TTAGTGGAGC AGCCAAGTAT 
CTCAGTGACA CCAAGAAATC CTTCAAACAG CAGAGACTGA CCCTACATTA TCATTCAACC 
ACCAAACGTG AAGAACTGAC TGTGAACCAC TTGCCATCTG AAAATATAAC CCACTATGAA 
GTGTTTGAGA ATGATGCAGC GACACATGTG GTGACAGCTG TGCTGTATGG GGCCAATGCT 
TGCTTTGTTT TTGACAGAGA AGCTTCATTA GTTGAAGACC AAAGCACTGT AGACAGAGAC 
GTAAAGGCTG CCTTAGAAAA ACTAAAAGGG ATTTCAGAAA GTACACAGAT TGATCTGAGC 
TTGGATGACA ATCAAAAGAC TGTTTTTGAG AAATTCAGCT GCACATTTTA TGGTGATTTC 
CAGTTAAAAT CTAATCCAGC CTCTTTTGAA GAAGCTCTGA GGGTTTTTGC TGATCTTCCT 
AAACTGCTGG GAGAAACCAA GGAGTGTGCA GTTCCACTGA GAGTGTGGCT TTATCCACTG 
GACAAACTGC ATTCACATTA TGTGAAACTT CAACAAGAAA TCAGCATAGA TCTAATCAGA 
AATGTTGAAT CAGTATTTGA GAGCTTAAGG ACCACTGAAA TGAAATGCAG TGATCTTCTG 
AATGATACAC CATCTTTGGC TTTTGCAGGA TTTCATGATA AAATAATGAT TATGAAGCAA 
AACTGCTGCA ACTATAAGCT GAGCTTCATG AAGAAACTTG GATCGCTATT GCCAAAAATC 
CGTAGTGATA CAGAAAATGA GACAGCCCTG ATTGAGCTCC TCCATGATCA TGAAAAATGT 
CCATTCAGAA AAAGGGATCT TGAGAAATGG GTGAAAGAAA AAGAGAATGA ATCCGTCATT 
ATTAAAACTC TTCTCAGACA ATTGACTGAT TCTGGAGCAA CAGTGGAAAA TAATCTAGAT 
GGAATTTTAA TGGATCTTAA TGTTGAAAAT GTAGTAAGCT ACACTTTCAC ATCACTTGAG 
TGTCCAGATG TGCTCCTTAC TAAACAAAAG GCTTTCCTGA ATCCATCAAC AATAGAAAAC 
AACAGTGAGG ATGCTTCTGT TTTAAGTAAA AAATCTGGGT TCACATCTGA CATCAAAACC 
ACAATGAGGA GCAACTTGAT CATCTTCAAA AACTTGATGG AATCAAAAAT ATGTAAACCA 
GCCAAATATA TTGTTGCATC GAAGGAAATA CCAAATCCAG GTTCCTGCAT TCTCATTTAC 
GAAAATGGAT CTGATGAAGC GACATGCTTT ACTCCGCCAA TAAAACCAGC TCATCCAGTC 
ACTGAACAGA TCAGTGGGGA AAGAGTGGTT TTAAAGGTGA CACCTGTGTG TCCTGCAACA 
GAAGAGCTCA GGTTACTGTA CAAAACAAAA GAAGAGAAAG ACTGGAAATC TCAGTCTGTG 
AAAAAGAGTC AAGATACAGT TATTCTGACT GATCTCAGAC CAGACACTGA GTATGAGATG 
AAATTTGCAG CCGTGGGGAA GCTGAACTAC ACCATAGACA GTGATGTGAT TCACCTCAGA 
GTTATTGACA AAAACCTCAC TGATGCCACA GAGTCCATTT TAGAAAAGCT GAGCTTGATA 
GAAGACAAGT GCAGTAAACT GATGGAGGGC AAGAGTGCAG TCACATTTAG AGCAATTCGC 
AACAAGTTTG AAGACATGAA GAGACACTGT CAGACTTACA AACAAGACAG AATTAAATCC 
ACAATCCAGT CAGTTCAAGC CTGTGAAAAA GACATCAGTG CTCTGACAGA CCTTCTACAA 
GCTCATCATG AGTCTCCATT TAACATAAGT GATATTGTGG AGTGGATCAG AGTGAAAGAA 
ACCGAATCAA AAGCAGTTGA GAAGATTCTT CAACAGCTGC TGGAAACTGG AGCTGAAGTG 
AACAATAGTT TAGATGAATA TTTGTTGGAT GTTAGTTTTG AGAATGTGGT GTGTTACACA 
TTCACTTCTC TTGATCTGCC AGACGACCTG CTTTCTGATC AAGAAGATTT CCTGAAACCT 
GAAATGATAA GGAGAAATTC TGATAAGAAA CCCAATGCTG TGTCTGAAAC TTGGCTCACA 
GGAAGTACCA GAGAGAAATT GAAAAAACAA TTAGAAATGT TTAAAGAGCT CAAATGTTCA 
TATGGCAGTC ACTCAACAAA GTTTTTGGTT ATATCAGAAG ATCAGACAAA CCATCCAGGT 
TCCTGTATTC TCCTTTATGA AAATGGATCT TATAAAGCGA AATGCTTTAC TCCGCCAATA 
AAACCAGCTG AGCCCATCAT TGAACAGGTC ATCGGGGAAA GAGTGGTTTT GAAGGTGACA 
TCTGTGTGTC CTGCAACAGA AGAGCTCAGG TTACTGTACA AAACAAAAGA AGAGAACGAC 
TGGAAATCTC AGTCTGTGAA AAAGAGTCAA GATACAGTTA TTCTGACTGA TCTCAGACCA 
GACACTGAGT ATGAGATGAA ATTTGCAGCT GTGGGGAAGC TGAACTACAC CATAGACAGT 
GATGTGATTC ACCTTAGAGT TATTGACAAG AAACTCACTG ATGCCACAGA GTCCATTTTA 
GAAAAGCTGA GCTTGATAGA AGACAAGTGC AGTAAACTGA TGGAGGACAA GAGTGCAGTC 
ACTTTTAGAG CAATTCACAA CAAGTTTGAA GACATGAGAA GACACTGTCA GATCTACAAA 
CAAGACTTAG AAATTAAAAT TACATCCACA ATGCAGTCAG TTCAAACCTG TGAAAAAGAC 
ATCAGTGCTC TGACAGACCT TCTACAAGCT CATCATGAGT CTCCATTTAA TGAAAGTGAT 
CTTATAGAGT GGATCAGAGT GAAAGAAACC GAATCAAATG CAGTTGAGAA GATTCTTCAA 
CAGCTGCTGG ACTCTGGAAC TGAGGTGAAC AATAGTTTAG ATGAATATTT GTTTGATGTT 
AGTTTTGAGA ATGTGGTGTG TTACACATTC ACTTCTCTTG ATCTGCCAGA CAACCTGCTT 
TCTAAACAAG GAGATTTCCT GAAACCTGAA ATAATGAGGA GAAATTCTAA TAAGAACCCC 
AATTCTGTGT CTGAAACTTG GCTCACAGGA AGTATCAGAG AGACTATGAA GAAGCATTTG 
GAAATATTTA ATGAGCTCAA ATGTTCATAT GGCAGTCACT CATCAACATT TTTGGTTGTA 
TATAAAAGTC ACACAAACCA TCCAGGTTCC TGCATTCTTA TTTATGAAAA TGGATCTGAT 
AAAGCGACAT GCTTCACTCC TCCAATAAAA CCAGCTAGTC CAGTCATTGA ACAGATTGGT 
GAGGATATAG TGGTTTTGAA GGCAACACCA TTATGTCCTG CAACACAAGA GCTCAGGTTA 
CTGTACAAAA AAAAAGAAGA GAATGATTGG AAATCTCAGT CTGTGAAAAC GAGTGGAGAT 
GAAGTTTTTC TGACTGGTCT CAGACCTGAC ACTGAGTATA AGATGAAATA TGTGGCCTTG 
GGGAAGCTGA ACTACACCAC AGACAGTGAT GTCATCGGAG TTACTACACG TGTGCTAAAT 
GAGACTGGCA AATATCTGCA GGATAAATCT GAACAAAAAC TATCGATGAC AAAGGAGCCA 
AGGAACAACC AACCAATGTC ATTTGATTCT TCAGAGGAGG TCAAAGTGTG CACTGTTTTG 
GCTGGAAAGA CCAATAACTG TCAAACACAT TTTATTGCAT CACTGCGAGC CCGAATTGAG 
CATCTGACGG AAGTTCGTTC AGTAGACGAG AGTGACATTG TTTTAATTTT CTGTCCAATT 
GTTTCTCGAG CTGGAACTGA CATTGATGCA GCCATTCACA GATGCTATAC AAATGCCTCT 
AAGCTGACTG TTCTAGTGGT GCTTCACCAC ACGTTTGACC CAGAGAAAAC TGTATCAGAC 
AGCAGCAAAA TTGTCAACAG GGAGAATCTG CTCACAGTGG ACTGTCTGTT CTATGAGGAT 
GCAGGATTAC TGCAGTGTCA GAGGAATTCA GATGCATATG ACAAATCTGT CAACTGGTTA 
ATAGCGCAGG GCAAAAAAAC AGGTCTTAAA ATCTACCCAA AGAAATCACC TGATAGTCAA 
TCCAATGGTC GGAAATTCTG GAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299674.1
CDS	617..5083
Translation	MSMHNSKISKYDASQCMKKQQLKKILPSPLINAWIYVLEVTIEIMDLGVGVNIVETAALGRPFRLGMLYDCRKDAFVPGITLWDEEQLQQSIKIHPQIKTDFCVTNSDFIEAKCQLLNINDCLKLSFLSGLINVSGAAKYLSDTKKSFKQQRLTLHYHSTTKREELTVNHLPSENITHYEVFENDAATHVVTAVLYGANACFVFDREASLVEDQSTVDRDVKAALEKLKGISESTQIDLSLDDNQKTVFEKFSCTFYGDFQLKSNPASFEEALRVFADLPKLLGETKECAVPLRVWLYPLDKLHSHYVKLQQEISIDLIRNVESVFESLRTTEMKCSDLLNDTPSLAFAGFHDKIMIMKQNCCNYKLSFMKKLGSLLPKIRSDTENETALIELLHDHEKCPFRKRDLEKWVKEKENESVIIKTLLRQLTDSGATVENNLDGILMDLNVENVVSYTFTSLECPDVLLTKQKAFLNPSTIENNSEDASVLSKKSGFTSDIKTTMRSNLIIFKNLMESKICKPAKYIVASKEIPNPGSCILIYENGSDEATCFTPPIKPAHPVTEQISGERVVLKVTPVCPATEELRLLYKTKEEKDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKNLTDATESILEKLSLIEDKCSKLMEGKSAVTFRAIRNKFEDMKRHCQTYKQDRIKSTIQSVQACEKDISALTDLLQAHHESPFNISDIVEWIRVKETESKAVEKILQQLLETGAEVNNSLDEYLLDVSFENVVCYTFTSLDLPDDLLSDQEDFLKPEMIRRNSDKKPNAVSETWLTGSTREKLKKQLEMFKELKCSYGSHSTKFLVISEDQTNHPGSCILLYENGSYKAKCFTPPIKPAEPIIEQVIGERVVLKVTSVCPATEELRLLYKTKEENDWKSQSVKKSQDTVILTDLRPDTEYEMKFAAVGKLNYTIDSDVIHLRVIDKKLTDATESILEKLSLIEDKCSKLMEDKSAVTFRAIHNKFEDMRRHCQIYKQDLEIKITSTMQSVQTCEKDISALTDLLQAHHESPFNESDLIEWIRVKETESNAVEKILQQLLDSGTEVNNSLDEYLFDVSFENVVCYTFTSLDLPDNLLSKQGDFLKPEIMRRNSNKNPNSVSETWLTGSIRETMKKHLEIFNELKCSYGSHSSTFLVVYKSHTNHPGSCILIYENGSDKATCFTPPIKPASPVIEQIGEDIVVLKATPLCPATQELRLLYKKKEENDWKSQSVKTSGDEVFLTGLRPDTEYKMKYVALGKLNYTTDSDVIGVTTRVLNETGKYLQDKSEQKLSMTKEPRNNQPMSFDSSEEVKVCTVLAGKTNNCQTHFIASLRARIEHLTEVRSVDESDIVLIFCPIVSRAGTDIDAAIHRCYTNASKLTVLVVLHHTFDPEKTVSDSSKIVNRENLLTVDCLFYEDAGLLQCQRNSDAYDKSVNWLIAQGKKTGLKIYPKKSPDSQSNGRKFWR

Target ORF information:

RefSeq Version	XM_009301399.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio uncharacterized LOC100148510 (LOC100148510), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301399.2

ORF Insert Sequence:

ATGTCCATGC ACAACTCAAA AATAAGCAAA TATGACGCAT CACAATGTAT GAAAAAACAA 
CAATTAAAAA AAATCTTGCC ATCGCCACTT ATAAATGCTT GGATTTACGT GCTTGAGGTT 
ACCATAGAAA TTATGGACTT GGGAGTGGGA GTTAACATTG TTGAAACAGC AGCTCTGGGG 
AGACCCTTCA GGCTGGGGAT GCTGTACGAC TGCAGAAAAG ATGCATTTGT ACCAGGAATC 
ACACTGTGGG ATGAAGAGCA ACTGCAGCAG AGCATAAAAA TCCATCCTCA AATTAAAACA 
GATTTCTGTG TTACAAATTC AGACTTTATT GAGGCAAAAT GCCAATTACT GAACATTAAT 
GATTGTCTAA AACTGAGTTT TTTAAGTGGA CTCATCAATG TTAGTGGAGC AGCCAAGTAT 
CTCAGTGACA CCAAGAAATC CTTCAAACAG CAGAGACTGA CCCTACATTA TCATTCAACC 
ACCAAACGTG AAGAACTGAC TGTGAACCAC TTGCCATCTG AAAATATAAC CCACTATGAA 
GTGTTTGAGA ATGATGCAGC GACACATGTG GTGACAGCTG TGCTGTATGG GGCCAATGCT 
TGCTTTGTTT TTGACAGAGA AGCTTCATTA GTTGAAGACC AAAGCACTGT AGACAGAGAC 
GTAAAGGCTG CCTTAGAAAA ACTAAAAGGG ATTTCAGAAA GTACACAGAT TGATCTGAGC 
TTGGATGACA ATCAAAAGAC TGTTTTTGAG AAATTCAGCT GCACATTTTA TGGTGATTTC 
CAGTTAAAAT CTAATCCAGC CTCTTTTGAA GAAGCTCTGA GGGTTTTTGC TGATCTTCCT 
AAACTGCTGG GAGAAACCAA GGAGTGTGCA GTTCCACTGA GAGTGTGGCT TTATCCACTG 
GACAAACTGC ATTCACATTA TGTGAAACTT CAACAAGAAA TCAGCATAGA TCTAATCAGA 
AATGTTGAAT CAGTATTTGA GAGCTTAAGG ACCACTGAAA TGAAATGCAG TGATCTTCTG 
AATGATACAC CATCTTTGGC TTTTGCAGGA TTTCATGATA AAATAATGAT TATGAAGCAA 
AACTGCTGCA ACTATAAGCT GAGCTTCATG AAGAAACTTG GATCGCTATT GCCAAAAATC 
CGTAGTGATA CAGAAAATGA GACAGCCCTG ATTGAGCTCC TCCATGATCA TGAAAAATGT 
CCATTCAGAA AAAGGGATCT TGAGAAATGG GTGAAAGAAA AAGAGAATGA ATCCGTCATT 
ATTAAAACTC TTCTCAGACA ATTGACTGAT TCTGGAGCAA CAGTGGAAAA TAATCTAGAT 
GGAATTTTAA TGGATCTTAA TGTTGAAAAT GTAGTAAGCT ACACTTTCAC ATCACTTGAG 
TGTCCAGATG TGCTCCTTAC TAAACAAAAG GCTTTCCTGA ATCCATCAAC AATAGAAAAC 
AACAGTGAGG ATGCTTCTGT TTTAAGTAAA AAATCTGGGT TCACATCTGA CATCAAAACC 
ACAATGAGGA GCAACTTGAT CATCTTCAAA AACTTGATGG AATCAAAAAT ATGTAAACCA 
GCCAAATATA TTGTTGCATC GAAGGAAATA CCAAATCCAG GTTCCTGCAT TCTCATTTAC 
GAAAATGGAT CTGATGAAGC GACATGCTTT ACTCCGCCAA TAAAACCAGC TCATCCAGTC 
ACTGAACAGA TCAGTGGGGA AAGAGTGGTT TTAAAGGTGA CACCTGTGTG TCCTGCAACA 
GAAGAGCTCA GGTTACTGTA CAAAACAAAA GAAGAGAAAG ACTGGAAATC TCAGTCTGTG 
AAAAAGAGTC AAGATACAGT TATTCTGACT GATCTCAGAC CAGACACTGA GTATGAGATG 
AAATTTGCAG CCGTGGGGAA GCTGAACTAC ACCATAGACA GTGATGTGAT TCACCTCAGA 
GTTATTGACA AAAACCTCAC TGATGCCACA GAGTCCATTT TAGAAAAGCT GAGCTTGATA 
GAAGACAAGT GCAGTAAACT GATGGAGGGC AAGAGTGCAG TCACATTTAG AGCAATTCGC 
AACAAGTTTG AAGACATGAA GAGACACTGT CAGACTTACA AACAAGACAG AATTAAATCC 
ACAATCCAGT CAGTTCAAGC CTGTGAAAAA GACATCAGTG CTCTGACAGA CCTTCTACAA 
GCTCATCATG AGTCTCCATT TAACATAAGT GATATTGTGG AGTGGATCAG AGTGAAAGAA 
ACCGAATCAA AAGCAGTTGA GAAGATTCTT CAACAGCTGC TGGAAACTGG AGCTGAAGTG 
AACAATAGTT TAGATGAATA TTTGTTGGAT GTTAGTTTTG AGAATGTGGT GTGTTACACA 
TTCACTTCTC TTGATCTGCC AGACGACCTG CTTTCTGATC AAGAAGATTT CCTGAAACCT 
GAAATGATAA GGAGAAATTC TGATAAGAAA CCCAATGCTG TGTCTGAAAC TTGGCTCACA 
GGAAGTACCA GAGAGAAATT GAAAAAACAA TTAGAAATGT TTAAAGAGCT CAAATGTTCA 
TATGGCAGTC ACTCAACAAA GTTTTTGGTT ATATCAGAAG ATCAGACAAA CCATCCAGGT 
TCCTGTATTC TCCTTTATGA AAATGGATCT TATAAAGCGA AATGCTTTAC TCCGCCAATA 
AAACCAGCTG AGCCCATCAT TGAACAGGTC ATCGGGGAAA GAGTGGTTTT GAAGGTGACA 
TCTGTGTGTC CTGCAACAGA AGAGCTCAGG TTACTGTACA AAACAAAAGA AGAGAACGAC 
TGGAAATCTC AGTCTGTGAA AAAGAGTCAA GATACAGTTA TTCTGACTGA TCTCAGACCA 
GACACTGAGT ATGAGATGAA ATTTGCAGCT GTGGGGAAGC TGAACTACAC CATAGACAGT 
GATGTGATTC ACCTTAGAGT TATTGACAAG AAACTCACTG ATGCCACAGA GTCCATTTTA 
GAAAAGCTGA GCTTGATAGA AGACAAGTGC AGTAAACTGA TGGAGGACAA GAGTGCAGTC 
ACTTTTAGAG CAATTCACAA CAAGTTTGAA GACATGAGAA GACACTGTCA GATCTACAAA 
CAAGACTTAG AAATTAAAAT TACATCCACA ATGCAGTCAG TTCAAACCTG TGAAAAAGAC 
ATCAGTGCTC TGACAGACCT TCTACAAGCT CATCATGAGT CTCCATTTAA TGAAAGTGAT 
CTTATAGAGT GGATCAGAGT GAAAGAAACC GAATCAAATG CAGTTGAGAA GATTCTTCAA 
CAGCTGCTGG ACTCTGGAAC TGAGGTGAAC AATAGTTTAG ATGAATATTT GTTTGATGTT 
AGTTTTGAGA ATGTGGTGTG TTACACATTC ACTTCTCTTG ATCTGCCAGA CAACCTGCTT 
TCTAAACAAG GAGATTTCCT GAAACCTGAA ATAATGAGGA GAAATTCTAA TAAGAACCCC 
AATTCTGTGT CTGAAACTTG GCTCACAGGA AGTATCAGAG AGACTATGAA GAAGCATTTG 
GAAATATTTA ATGAGCTCAA ATGTTCATAT GGCAGTCACT CATCAACATT TTTGGTTGTA 
TATAAAAGTC ACACAAACCA TCCAGGTTCC TGCATTCTTA TTTATGAAAA TGGATCTGAT 
AAAGCGACAT GCTTCACTCC TCCAATAAAA CCAGCTAGTC CAGTCATTGA ACAGATTGGT 
GAGGATATAG TGGTTTTGAA GGCAACACCA TTATGTCCTG CAACACAAGA GCTCAGGTTA 
CTGTACAAAA AAAAAGAAGA GAATGATTGG AAATCTCAGT CTGTGAAAAC GAGTGGAGAT 
GAAGTTTTTC TGACTGGTCT CAGACCTGAC ACTGAGTATA AGATGAAATA TGTGGCCTTG 
GGGAAGCTGA ACTACACCAC AGACAGTGAT GTCATCGGAG TTACTACACG TGTGCTAAAT 
GAGACTGGCA AATATCTGCA GGATAAATCT GAACAAAAAC TATCGATGAC AAAGGAGCCA 
AGGAACAACC AACCAATGTC ATTTGATTCT TCAGAGGAGG TCAAAGTGTG CACTGTTTTG 
GCTGGAAAGA CCAATAACTG TCAAACACAT TTTATTGCAT CACTGCGAGC CCGAATTGAG 
CATCTGACGG AAGTTCGTTC AGTAGACGAG AGTGACATTG TTTTAATTTT CTGTCCAATT 
GTTTCTCGAG CTGGAACTGA CATTGATGCA GCCATTCACA GATGCTATAC AAATGCCTCT 
AAGCTGACTG TTCTAGTGGT GCTTCACCAC ACGTTTGACC CAGAGAAAAC TGTATCAGAC 
AGCAGCAAAA TTGTCAACAG GGAGAATCTG CTCACAGTGG ACTGTCTGTT CTATGAGGAT 
GCAGGATTAC TGCAGTGTCA GAGGAATTCA GATGCATATG ACAAATCTGT CAACTGGTTA 
ATAGCGCAGG GCAAAAAAAC AGGTCTTAAA ATCTACCCAA AGAAATCACC TGATAGTCAA 
TCCAATGGTC GGAAATTCTG GAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Gene Symbol	LOC100148510
Entrez Gene ID	100148510
Full Name	uncharacterized LOC100148510
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

LOC100148510 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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