tcf20 cDNA ORF clone, Danio rerio(zebrafish)

The following tcf20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tcf20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa07773 XM_009299007.2
Latest version!
Danio rerio transcription factor 20 (tcf20), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa07773 XM_021472464.1
Latest version!
Danio rerio transcription factor 20 (tcf20), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa07773 XM_005171536.4
Latest version!
Danio rerio transcription factor 20 (tcf20), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
View Old Accession Versions >>
ODa07773 XM_005171536.3 Danio rerio transcription factor 20 (tcf20), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa07773
    Clone ID Related Accession (Same CDS sequence) XM_021472464.1 , XM_005171536.3 , XM_009299007.2 , XM_005171536.4
    Accession Version XM_021472464.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product transcription factor 20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCAGAATT TCCCCAACAG TCCTGTCCCT CTCCACCCAG GGTTCAGCCG TGGAGCAGGC 
    GTAGTCAGTT CCCCATATCA GACCCACCCG TCAGACCTTC AGATCTCCCC CAGGACCTCC
    GAGGACTATG TGTCCCTTCA GCAGGCCCAG ACCAGCCTAC AGAGCCACGG GCAGCACTTG
    CACCTGCGGG CCCAGCAGCA GCAGCACCTG CATGCTCCGG TACATGGCTA CAGCTCCAGG
    CGAGCGGTCA CAGAGATGCC GCAGAGCAGC GCGGGGGGGA ACTCCTACCG AAAGGACAGT
    ATGGATTATT ACTTCTCTGT GAGTGGGCGA GACCGAAGCC GAAGAGGTGG AGCCGCTTAC
    GGAGCCGGAT TTAGGTACAC AAACGTGGAT GGGCACCAGT ACCAACACCT CTCCGGTTCA
    GGGTCTTCCT CCGGAATGAT TTCTCCCTAC TCCATGGACT GTGGCTCTGC AGTCGTGGGG
    AGTAATAGCA GTGCAGCTGG TTCGTTTTCT CCTTCCCAGC AATACAGTAT GGCTCACGCT
    GCTTCAGTAC AATCAAGTGC TCAAATGCAT CAACGGCAGA AATATCCCTC ACATCAAGGC
    TTATTACACC AAGGTCAGCA TCACCGGACA TATGCGCTCT CCGGAAACAG AATCCCCGCT
    CAGTTCGGAC ACTACGCCAC TATCAATGCA ACCTCAGGCT CCTCTGGAAT GTATAACTCT
    CCACCGCAGA GATACGACGT GAGCAGCAGT AGCAGCACAG AAGCCAAAAT CAATGACTCT
    CCCACGCAGT CTAATCCAAA TCATCATAGC AGTTCATCAG CTTCCAACAG CTGCGAGGCT
    ATGGGGCAGG GCTACTCCTC GTCTGCACAT CCACCGTATT CACCACAGTC GCTTCACAAG
    CACGGCCCCC ATTCCCAGCG TACCCCTCAA CACAGCGCTG TCGCAGGCTA TGACCTATCC
    CTCAAAATGC AGCATCTGCA CACCAAACTG CCCCATTCCT CTGTCTCCTC CAGCCCGTCT
    CTGCCCTCAC ACACCCCTCA AGACCTCACC AAGTCTCCTA TGCACACTCA AAATCAACAG
    GCTCAGATTC ATCAGAACTT CAGTCCTATC TCTAATCCCT CCCCAGCTCC ATCTGCGGTG
    CAGTCTCCAA GCTGCAGCTC GTCCTCATCT CCACTGCTAG GGGTCTCGGA GGGAAACAGT
    GCTCAGCTGC AGGCGTCTTC ACACCCTGCT GCCCCAAACG CACGCAGCAG CCAGAGTCAC
    GCACGCCTCC TGCAGGCCGT CCCCCAGCTG AGCCCCACTC CCCATTCCAA TAGCAGCATC
    AGTAGCTGTG GTAGCGGAGG TGGAAATAAG TCTGCCACTC TGAATCACAG TGCAGGGATT
    GGTCATGCCA ATATGGGGCA GAACCGAATG GGGCCAGGTC TCCGGAGTGG TCCGGGGGAA
    GATAGCTCCC TGTTCCCTCA TGACAAACAC CTACCGGACC CTGGTCTAAA CAGTCTCAAT
    GCTCTGACTT CTCAAGTGGA AAACCTACCG AATACAGTCC AGCACATGCT GCTAACCGAC
    ACTGTGCTGC CCCAGAGGAA GAGCAGAGAC GCTGCCCCTC ACTTTCAGAT GCAGCAGGGG
    TCTCACTCTT TACCTGGCAA CCAGAACAAA TCTGGGAACT CCAGCACTGC TGGTCGGATC
    TTAACAAATG AAGAGAGCTC TGAGATGCTG GAAACAAAAG GCCAACAAAA ATTAGAAAGG
    AAAAGAGTTA GGCAGGAAAG TGGTACAAGC AATGAATCTG AGCCACAGAG CTACCTGCCA
    TCACAAAGGC AGATGCCCTC AGATAAGAGT CAGCAGGGCC TGGATCTCCA GGTCAACACT
    GGAGTCATCT CAACAGCTTC AAAACAGTCT TCTGTTAGAG AGTCTCAAGC AAAGACTCCA
    GAAACCCTCA CCCCCTCCTC CACTTCACCA CCATCAGGTC ATCCTACTGC AGAACCCAGC
    CCAAAGCTAC AGGTGTATCC CCCTGCTCCA GCCTCTCCCA TTCACTCCGC ATGTCTAAAC
    TCTGTGGCAG ATAGAGACCT AGGTAAAGAT GATGAGAAGG GAACTAAGGG ACCACAAATC
    AAAATGATAA AAGATGAAGA AAGCGATGAT GAATGTGCAG ATCCTCCGTG TCATAGAACG
    AGTTCAGAGA ACAGACTGTT GGTTCCTCTC AAACAGGCCG AGCGAGAGTC CTGTGCTCAA
    TCATTAGAAA ATCCCAGTAA AAATGTGAAT GTTCCAGAAC AACACAATGT TGGAGGTGTA
    GGGGTTATTG TCTCTGCTCG TTCTGAACTG AACACAGAAT CAAGCAAGCA CACAGGAGTC
    AAATCTCCAC GTTACAGTGT TTCTCATTAC CCAAACAAAC CCAGCAGTAT TGAGGAGCAG
    CATGATGTCA ATGTCTTAAA AGAGACGAGA AACCAGAATG GAGAAGGAGA GATGTCCATG
    GAAGCATATG TGTCACAGTA TGATATTTCC CCTAAACGAG AATTTGGCCA AAACATTGTT
    TCTGTTCAAT CTCACCCAGT GTCATTTAAA TGCAGCAATC CAGAGGTACA ATACACTGCC
    GTAAAGAGCA AGGACAAATT AGGGCTAGGT AGTGTTATGG GTGCAAACAG AGGCCAGAAT
    TACCATACAC TACAGGCCAG CTATGGGTCT ATTGCCAGAA AGGAGATTGC TGGTGTATTT
    GCTATGGAGG GGGGAAGAGG AATGGTCTCA AGAAGCCAGG ATAACAATTC TCAGTTTCAG
    CAGGTATTTC CAAGCCTTTT ACAAGAAGTC CTTCAAGGCC ATCACTTGGA TCGCCGCTAT
    GTACGTGCTG AACAGACATC TAGTGCTCAT CAACATTCTC AAGATACGTC TCAGCACCGT
    TTTCAAACCA GGCTACCTTA CAGTATGATT GAAAACTTGA GTTCACATTC AATGGGCTCT
    CAATCAGTTA TGGGGGGACT TAATGTCCAG TTTAGTCAAT TGGCCCCTGG AAAATCTCCA
    AACTCAAGTC AGGGACCAGA TAGTGAGATT GGTTTAGGCC CTTCTCATCC CTCTTGGGAT
    TCTGAAGCAC ATAAGACTCA CGGGATCTCC ATAGAGAAAA GCAAAACTAG TCTGTCTCCC
    AGCCAGTCCT CCCACATGCA GCAGTCTTTA GATCTCTCAA CAGGAGCCCC TCCAAAGCAC
    ATCAACCTGG CTGACTATTC TTTGCAGCAC AGAAAACCAT CCAGATATGG TACCTCTCCT
    TCAGCTGTAG AACAATTGCT TTTACAGGAA GCTGAGCCAC TGGCATGCAA TGTAGGTCCT
    GTCGGCCATG TTCAATCCCA GGCATCCTCA TCCTCTGGAA GGCGCTCGGT AATCTGTGAT
    GTGTCGCCTT CTCGACGAAC AACACCAGAA AGAGAGAGAG GATCCTCTGG ACCCTCAGTC
    ATTCAGCAGC CGTTTTCTTC TGGGTCTAAT GAGCAAGACT GCTGCAAGGA AGAAATTAAA
    GCAAAGAAAG CACAAATCAA GGAGGACCCA TCAAAGGTTG AAGCTGCAGG TCAGAACACA
    GATCGCTACT GCAGCACACC ACCCAGTAAG GAGTCTAATA AGCCTCTTCG CCATCCTGTA
    GATAGTGATC CTGACTTAGA AAAGACCAGC AGTGCCAAGG GCAACAGTGA TGCCCTCAGC
    AACCTGCCAT ATCTTTCACA AATGCCCACA AACCCCTTGT CCTCACCACA GAGACTCCAG
    TCATTTTCAC AGGCTGTTGA TGGCTTAAGG GCATATGGCT TCGGTGATGC AATGGATGGA
    CAAAAAATGA TCCCTCATGC TGGACCACAT CAACCGTTCC ATACGCTATC AGCATATACT
    AAGACCACAG CCTCTACAAA CAAGTTACAA GTGTTTCCAC AGAGTTTGCC TCCTCAAGAC
    AGACCTGACT GGACTCCTGA CAGACAAAAG CTTCGAGGTA TGGACAGGCA CGGTCCTCAG
    AGAATCCCCG AACAGAAATG TAAAAGCCAG CCTTCAAGTG AAATTCTGTC TAGTCCACAT
    CGTATATCTC AACAGCACTC TTATCCTGGT GCACAGTTTG ACCTGAAAAT GTGGGATGCT
    TATCCTGAAA AAGAGGGAGC TGCGCAACCA CTGGCTGCAG TCCCTCGTGA GCATGTTGGT
    TCTCAACCAA TTACAAATTC TGGCCCCAAA ATTGCAAAAG AGGACATCTC AGATAAGGGT
    GCAGAAGACA CAGTCAAATC CTTCCACTCA TTGGCACCGG CTAGTACTAA TGCTGGGCAC
    ACCGTCAAGG CAACCCAAGG CATTCAGCAG GGGCAGAGAG TAACTAAAAC TGGAGCATTG
    GCTGAAACTA ATCCCCTAAT CATGAGAAGG AGAGTTAGAT CTTTTATTTC CCCTATTCCT
    GCTAAGAGGC AGCATCAGGA TGTTCCAGGT CAGCGGCCAG GATCAGCATA CCACTCCCCA
    ATAGCTCAAG CCTACCCTGA ATCTAGAATT GCAACCAACA GTTGTTCTGG TAGTTCGGAT
    TCTCAGGCCA AATTACCATC TCCCAAGACA CCGCATTCCA TATCTAGTGC TAACTCGCCT
    CCTCAGTCCA AAGCAAAGTT TCTTCCCCCA AGAAAGGGGC GTGGCTTGAA ACTTGAGGCG
    ATTGTGCAGA AAATTACTCC TGGCGTGAGA AAAAATGACT TCAACAATAG TAACTTGGAA
    TCGGACTTTT CAGAAGCAGC TCATTACACC TCTGACTTGC CGGACCCAGA GAGTGGTACA
    TCGTTTACTA GTGTTTCTCA AGGTGAGGAG ACCTGTTTAT CTTACCTTGA GGATTCTCAT
    ACTTTAGATG AGCTTATGCC ATACAGAGCA GTTGAAGATG CATTCTCATG TGACTCTCAG
    TCTCTCAAAG CAGGTGCAGC TGCTTCTAGC AGCGGTGCCC TCAGAAGTTT GCCAAAAGAT
    TTCGACTTTG GCTTAGGTAC TGCTGGCTCT TCAGGGTCTT TGGGGGGTGA AAATGATAAG
    GATGAATTTA CTTTGTTGGG ACCCCTTCCA CCAGCTCCTC CATTGCCTTG TCCTGTACAG
    GGATCACCTC CTCCATCTTC ATCTGCATTG TCAGACATAC AACAATTCAC AAACACTTAT
    CAACAGCTGG AAACAAGGCG AAGTGAGCAG TCGGCTGCTA ACCTGCTGAG GCAGAAGTTG
    CAGGAGACTG GCATGGGCTT TGACGATTAC CCAGGAGGGG ATTACTATGG GACCACTGCT
    GGCCATAGTC CTGGACATCA CCTACTGTCC CGAGGTTCAC AAAACCAGAT GACTTCTCTC
    AGGTTGACAA GTCCAGACTC TAAAGCATCA GAAAGCACTG TGCCCAAAGG TTATTTCCCA
    TCAGGAAAGA AAAAAGGCCG CCCAGTCGGT AGTGTGAATA AGCAGAAACG TGCCCAAGGA
    CAAATGCAGA ATACAGCAAC TAACATACCT GCAGCTCCTC TTCCCTCACC TACAGCTGCA
    CCTCAGTCTG TTTCAGTCTC AAGCAATGCT GATGAAGTGC CCCGAGTACC AACACCACCT
    GATGAGAAAC CCTGCCCATC TCTGGTCCCT CCCGATGAAA CCCAAGTGGT GAAGGTGGAT
    GCAGAAAGCG AGGAGACCCT GCCAGAGTTG GATGTAAAGC CTGTTAAACA AAAACAGAAA
    AAGGGGAAAG AGTGCAGCGA GACTCCTGCC TCAAGTGGCA ATCAGAGAAG GAGAAGAAGA
    GGAATGGCTT CCAAAGAGGA GCTGGACACT CAGACGAGCA GTAGGGGAAG CATTAGTCTC
    AGTGGAATAT TCTCTGATGC CAGGAATAGT GTTTTTGCTC CTTACGTACA TGTGGAGAAA
    AAGATGGAAG AAATTGGGGC AGTCTGTACA ATAATTAACG CAGAGGATGA AAAGTCAAAA
    GGTGGAGGCA AACCTGGTCA CACGGGGGTT GATGGCCCTT TAAACACCCT GCTGCCATCT
    CAGCTTGTGA GAAAAGAAAA GGAGAATGAA AAATCAAAAG AGAACTGGGT TTCAGAGCAA
    GTTGATTCAA CCTTGGAGTC AGGGAAAGCA CTTCCTACAT CCGAGTATGT TCTGCCAGGG
    CCTGTGATAT CCGAAACTGG ACACAGCGGT CACCTGTTGT GCTGCCTTTG TCAGAAATGG
    GCCAACTACA AACATCTTGG AGATCTCTAT GGTCCTTTTT ACCCAGCCGA GTATGCAGCC
    AAGCTCCCTA AGAATCAACC TCAAGTCCGA CAAACTTTGT CCCATTTTGG GCCAGCTGCC
    ACAGGTATGA CATCCATTCC AACAGAAGCG ATTCCCCAGG ATACAAGATT ACAAGAACCT
    CAGAATGTCA AGTCATCTGC AGAAAGCGAT TGCACAGTAA GCCAGGCAAC AAACACAACA
    TCCCCAGCCA CTATCATTGG CACTGTTTCT CCAATGCCTT TGGAAATGCC TTTCCAGAAT
    GCTATAGCCA GCAGCTCTAC TGCAAGGGTA ACAACTCATG CTTGGGATAC GGCAGCCGAG
    CTGGAAAGTG GACTGGGTAC ATCAAATTTA CAAGAAACAG ACGCTGAAAT CGCATCAAAG
    CAGTTGCAGG TAGAAAGTTC ACAGCAAAGG CCCCAGCACA GAAAACTGAC ATCTCATCCA
    CGCTTCAAAA GAAGACACAA ATCCAGCGAA GATTTGCCTC GAACTGTCCC AATTAACAGC
    AAAGCCTCCC TGCCCTTCCA GCCTCCACCC CCAAGCCTGG ATGCTCTGGG CCCCATGGCT
    CAGCTGGCGC AGCTCCCGCT GGTGCCTCTG GACCCTGAGG AGCTGTGGGT GCACGAGAGC
    TGCATGGTGT GGACCAGTGG CATATACCTG GTCAATGGGA GACTCTATGG CCTTCAGGAA
    GCACTGGATG GTGCTAGAGA TGCGAGCTGC TCTCATTGTG AGATGGCGGG CTCCACCCTC
    GGCTGCTACA GTAAAGGCTG CACGCTGAGG TACCACTACA TTTGTGCCAT AGACGCAGAT
    TGCTCTCTTA ACGAAGACAA CTTTTCTTTG AGGTGTCCAA AGCACAAGGG CAACAGGATG
    GCCAAGCCTG GAGTGGTGTA TCTGGAGCAG TCGGAGCGCG GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021328139.1
    CDS1040..8104
    Translation

    Target ORF information:

    RefSeq Version XM_021472464.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio transcription factor 20 (tcf20), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021472464.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCAGAATT TCCCCAACAG TCCTGTCCCT CTCCACCCAG GGTTCAGCCG TGGAGCAGGC 
    GTAGTCAGTT CCCCATATCA GACCCACCCG TCAGACCTTC AGATCTCCCC CAGGACCTCC
    GAGGACTATG TGTCCCTTCA GCAGGCCCAG ACCAGCCTAC AGAGCCACGG GCAGCACTTG
    CACCTGCGGG CCCAGCAGCA GCAGCACCTG CATGCTCCGG TACATGGCTA CAGCTCCAGG
    CGAGCGGTCA CAGAGATGCC GCAGAGCAGC GCGGGGGGGA ACTCCTACCG AAAGGACAGT
    ATGGATTATT ACTTCTCTGT GAGTGGGCGA GACCGAAGCC GAAGAGGTGG AGCCGCTTAC
    GGAGCCGGAT TTAGGTACAC AAACGTGGAT GGGCACCAGT ACCAACACCT CTCCGGTTCA
    GGGTCTTCCT CCGGAATGAT TTCTCCCTAC TCCATGGACT GTGGCTCTGC AGTCGTGGGG
    AGTAATAGCA GTGCAGCTGG TTCGTTTTCT CCTTCCCAGC AATACAGTAT GGCTCACGCT
    GCTTCAGTAC AATCAAGTGC TCAAATGCAT CAACGGCAGA AATATCCCTC ACATCAAGGC
    TTATTACACC AAGGTCAGCA TCACCGGACA TATGCGCTCT CCGGAAACAG AATCCCCGCT
    CAGTTCGGAC ACTACGCCAC TATCAATGCA ACCTCAGGCT CCTCTGGAAT GTATAACTCT
    CCACCGCAGA GATACGACGT GAGCAGCAGT AGCAGCACAG AAGCCAAAAT CAATGACTCT
    CCCACGCAGT CTAATCCAAA TCATCATAGC AGTTCATCAG CTTCCAACAG CTGCGAGGCT
    ATGGGGCAGG GCTACTCCTC GTCTGCACAT CCACCGTATT CACCACAGTC GCTTCACAAG
    CACGGCCCCC ATTCCCAGCG TACCCCTCAA CACAGCGCTG TCGCAGGCTA TGACCTATCC
    CTCAAAATGC AGCATCTGCA CACCAAACTG CCCCATTCCT CTGTCTCCTC CAGCCCGTCT
    CTGCCCTCAC ACACCCCTCA AGACCTCACC AAGTCTCCTA TGCACACTCA AAATCAACAG
    GCTCAGATTC ATCAGAACTT CAGTCCTATC TCTAATCCCT CCCCAGCTCC ATCTGCGGTG
    CAGTCTCCAA GCTGCAGCTC GTCCTCATCT CCACTGCTAG GGGTCTCGGA GGGAAACAGT
    GCTCAGCTGC AGGCGTCTTC ACACCCTGCT GCCCCAAACG CACGCAGCAG CCAGAGTCAC
    GCACGCCTCC TGCAGGCCGT CCCCCAGCTG AGCCCCACTC CCCATTCCAA TAGCAGCATC
    AGTAGCTGTG GTAGCGGAGG TGGAAATAAG TCTGCCACTC TGAATCACAG TGCAGGGATT
    GGTCATGCCA ATATGGGGCA GAACCGAATG GGGCCAGGTC TCCGGAGTGG TCCGGGGGAA
    GATAGCTCCC TGTTCCCTCA TGACAAACAC CTACCGGACC CTGGTCTAAA CAGTCTCAAT
    GCTCTGACTT CTCAAGTGGA AAACCTACCG AATACAGTCC AGCACATGCT GCTAACCGAC
    ACTGTGCTGC CCCAGAGGAA GAGCAGAGAC GCTGCCCCTC ACTTTCAGAT GCAGCAGGGG
    TCTCACTCTT TACCTGGCAA CCAGAACAAA TCTGGGAACT CCAGCACTGC TGGTCGGATC
    TTAACAAATG AAGAGAGCTC TGAGATGCTG GAAACAAAAG GCCAACAAAA ATTAGAAAGG
    AAAAGAGTTA GGCAGGAAAG TGGTACAAGC AATGAATCTG AGCCACAGAG CTACCTGCCA
    TCACAAAGGC AGATGCCCTC AGATAAGAGT CAGCAGGGCC TGGATCTCCA GGTCAACACT
    GGAGTCATCT CAACAGCTTC AAAACAGTCT TCTGTTAGAG AGTCTCAAGC AAAGACTCCA
    GAAACCCTCA CCCCCTCCTC CACTTCACCA CCATCAGGTC ATCCTACTGC AGAACCCAGC
    CCAAAGCTAC AGGTGTATCC CCCTGCTCCA GCCTCTCCCA TTCACTCCGC ATGTCTAAAC
    TCTGTGGCAG ATAGAGACCT AGGTAAAGAT GATGAGAAGG GAACTAAGGG ACCACAAATC
    AAAATGATAA AAGATGAAGA AAGCGATGAT GAATGTGCAG ATCCTCCGTG TCATAGAACG
    AGTTCAGAGA ACAGACTGTT GGTTCCTCTC AAACAGGCCG AGCGAGAGTC CTGTGCTCAA
    TCATTAGAAA ATCCCAGTAA AAATGTGAAT GTTCCAGAAC AACACAATGT TGGAGGTGTA
    GGGGTTATTG TCTCTGCTCG TTCTGAACTG AACACAGAAT CAAGCAAGCA CACAGGAGTC
    AAATCTCCAC GTTACAGTGT TTCTCATTAC CCAAACAAAC CCAGCAGTAT TGAGGAGCAG
    CATGATGTCA ATGTCTTAAA AGAGACGAGA AACCAGAATG GAGAAGGAGA GATGTCCATG
    GAAGCATATG TGTCACAGTA TGATATTTCC CCTAAACGAG AATTTGGCCA AAACATTGTT
    TCTGTTCAAT CTCACCCAGT GTCATTTAAA TGCAGCAATC CAGAGGTACA ATACACTGCC
    GTAAAGAGCA AGGACAAATT AGGGCTAGGT AGTGTTATGG GTGCAAACAG AGGCCAGAAT
    TACCATACAC TACAGGCCAG CTATGGGTCT ATTGCCAGAA AGGAGATTGC TGGTGTATTT
    GCTATGGAGG GGGGAAGAGG AATGGTCTCA AGAAGCCAGG ATAACAATTC TCAGTTTCAG
    CAGGTATTTC CAAGCCTTTT ACAAGAAGTC CTTCAAGGCC ATCACTTGGA TCGCCGCTAT
    GTACGTGCTG AACAGACATC TAGTGCTCAT CAACATTCTC AAGATACGTC TCAGCACCGT
    TTTCAAACCA GGCTACCTTA CAGTATGATT GAAAACTTGA GTTCACATTC AATGGGCTCT
    CAATCAGTTA TGGGGGGACT TAATGTCCAG TTTAGTCAAT TGGCCCCTGG AAAATCTCCA
    AACTCAAGTC AGGGACCAGA TAGTGAGATT GGTTTAGGCC CTTCTCATCC CTCTTGGGAT
    TCTGAAGCAC ATAAGACTCA CGGGATCTCC ATAGAGAAAA GCAAAACTAG TCTGTCTCCC
    AGCCAGTCCT CCCACATGCA GCAGTCTTTA GATCTCTCAA CAGGAGCCCC TCCAAAGCAC
    ATCAACCTGG CTGACTATTC TTTGCAGCAC AGAAAACCAT CCAGATATGG TACCTCTCCT
    TCAGCTGTAG AACAATTGCT TTTACAGGAA GCTGAGCCAC TGGCATGCAA TGTAGGTCCT
    GTCGGCCATG TTCAATCCCA GGCATCCTCA TCCTCTGGAA GGCGCTCGGT AATCTGTGAT
    GTGTCGCCTT CTCGACGAAC AACACCAGAA AGAGAGAGAG GATCCTCTGG ACCCTCAGTC
    ATTCAGCAGC CGTTTTCTTC TGGGTCTAAT GAGCAAGACT GCTGCAAGGA AGAAATTAAA
    GCAAAGAAAG CACAAATCAA GGAGGACCCA TCAAAGGTTG AAGCTGCAGG TCAGAACACA
    GATCGCTACT GCAGCACACC ACCCAGTAAG GAGTCTAATA AGCCTCTTCG CCATCCTGTA
    GATAGTGATC CTGACTTAGA AAAGACCAGC AGTGCCAAGG GCAACAGTGA TGCCCTCAGC
    AACCTGCCAT ATCTTTCACA AATGCCCACA AACCCCTTGT CCTCACCACA GAGACTCCAG
    TCATTTTCAC AGGCTGTTGA TGGCTTAAGG GCATATGGCT TCGGTGATGC AATGGATGGA
    CAAAAAATGA TCCCTCATGC TGGACCACAT CAACCGTTCC ATACGCTATC AGCATATACT
    AAGACCACAG CCTCTACAAA CAAGTTACAA GTGTTTCCAC AGAGTTTGCC TCCTCAAGAC
    AGACCTGACT GGACTCCTGA CAGACAAAAG CTTCGAGGTA TGGACAGGCA CGGTCCTCAG
    AGAATCCCCG AACAGAAATG TAAAAGCCAG CCTTCAAGTG AAATTCTGTC TAGTCCACAT
    CGTATATCTC AACAGCACTC TTATCCTGGT GCACAGTTTG ACCTGAAAAT GTGGGATGCT
    TATCCTGAAA AAGAGGGAGC TGCGCAACCA CTGGCTGCAG TCCCTCGTGA GCATGTTGGT
    TCTCAACCAA TTACAAATTC TGGCCCCAAA ATTGCAAAAG AGGACATCTC AGATAAGGGT
    GCAGAAGACA CAGTCAAATC CTTCCACTCA TTGGCACCGG CTAGTACTAA TGCTGGGCAC
    ACCGTCAAGG CAACCCAAGG CATTCAGCAG GGGCAGAGAG TAACTAAAAC TGGAGCATTG
    GCTGAAACTA ATCCCCTAAT CATGAGAAGG AGAGTTAGAT CTTTTATTTC CCCTATTCCT
    GCTAAGAGGC AGCATCAGGA TGTTCCAGGT CAGCGGCCAG GATCAGCATA CCACTCCCCA
    ATAGCTCAAG CCTACCCTGA ATCTAGAATT GCAACCAACA GTTGTTCTGG TAGTTCGGAT
    TCTCAGGCCA AATTACCATC TCCCAAGACA CCGCATTCCA TATCTAGTGC TAACTCGCCT
    CCTCAGTCCA AAGCAAAGTT TCTTCCCCCA AGAAAGGGGC GTGGCTTGAA ACTTGAGGCG
    ATTGTGCAGA AAATTACTCC TGGCGTGAGA AAAAATGACT TCAACAATAG TAACTTGGAA
    TCGGACTTTT CAGAAGCAGC TCATTACACC TCTGACTTGC CGGACCCAGA GAGTGGTACA
    TCGTTTACTA GTGTTTCTCA AGGTGAGGAG ACCTGTTTAT CTTACCTTGA GGATTCTCAT
    ACTTTAGATG AGCTTATGCC ATACAGAGCA GTTGAAGATG CATTCTCATG TGACTCTCAG
    TCTCTCAAAG CAGGTGCAGC TGCTTCTAGC AGCGGTGCCC TCAGAAGTTT GCCAAAAGAT
    TTCGACTTTG GCTTAGGTAC TGCTGGCTCT TCAGGGTCTT TGGGGGGTGA AAATGATAAG
    GATGAATTTA CTTTGTTGGG ACCCCTTCCA CCAGCTCCTC CATTGCCTTG TCCTGTACAG
    GGATCACCTC CTCCATCTTC ATCTGCATTG TCAGACATAC AACAATTCAC AAACACTTAT
    CAACAGCTGG AAACAAGGCG AAGTGAGCAG TCGGCTGCTA ACCTGCTGAG GCAGAAGTTG
    CAGGAGACTG GCATGGGCTT TGACGATTAC CCAGGAGGGG ATTACTATGG GACCACTGCT
    GGCCATAGTC CTGGACATCA CCTACTGTCC CGAGGTTCAC AAAACCAGAT GACTTCTCTC
    AGGTTGACAA GTCCAGACTC TAAAGCATCA GAAAGCACTG TGCCCAAAGG TTATTTCCCA
    TCAGGAAAGA AAAAAGGCCG CCCAGTCGGT AGTGTGAATA AGCAGAAACG TGCCCAAGGA
    CAAATGCAGA ATACAGCAAC TAACATACCT GCAGCTCCTC TTCCCTCACC TACAGCTGCA
    CCTCAGTCTG TTTCAGTCTC AAGCAATGCT GATGAAGTGC CCCGAGTACC AACACCACCT
    GATGAGAAAC CCTGCCCATC TCTGGTCCCT CCCGATGAAA CCCAAGTGGT GAAGGTGGAT
    GCAGAAAGCG AGGAGACCCT GCCAGAGTTG GATGTAAAGC CTGTTAAACA AAAACAGAAA
    AAGGGGAAAG AGTGCAGCGA GACTCCTGCC TCAAGTGGCA ATCAGAGAAG GAGAAGAAGA
    GGAATGGCTT CCAAAGAGGA GCTGGACACT CAGACGAGCA GTAGGGGAAG CATTAGTCTC
    AGTGGAATAT TCTCTGATGC CAGGAATAGT GTTTTTGCTC CTTACGTACA TGTGGAGAAA
    AAGATGGAAG AAATTGGGGC AGTCTGTACA ATAATTAACG CAGAGGATGA AAAGTCAAAA
    GGTGGAGGCA AACCTGGTCA CACGGGGGTT GATGGCCCTT TAAACACCCT GCTGCCATCT
    CAGCTTGTGA GAAAAGAAAA GGAGAATGAA AAATCAAAAG AGAACTGGGT TTCAGAGCAA
    GTTGATTCAA CCTTGGAGTC AGGGAAAGCA CTTCCTACAT CCGAGTATGT TCTGCCAGGG
    CCTGTGATAT CCGAAACTGG ACACAGCGGT CACCTGTTGT GCTGCCTTTG TCAGAAATGG
    GCCAACTACA AACATCTTGG AGATCTCTAT GGTCCTTTTT ACCCAGCCGA GTATGCAGCC
    AAGCTCCCTA AGAATCAACC TCAAGTCCGA CAAACTTTGT CCCATTTTGG GCCAGCTGCC
    ACAGGTATGA CATCCATTCC AACAGAAGCG ATTCCCCAGG ATACAAGATT ACAAGAACCT
    CAGAATGTCA AGTCATCTGC AGAAAGCGAT TGCACAGTAA GCCAGGCAAC AAACACAACA
    TCCCCAGCCA CTATCATTGG CACTGTTTCT CCAATGCCTT TGGAAATGCC TTTCCAGAAT
    GCTATAGCCA GCAGCTCTAC TGCAAGGGTA ACAACTCATG CTTGGGATAC GGCAGCCGAG
    CTGGAAAGTG GACTGGGTAC ATCAAATTTA CAAGAAACAG ACGCTGAAAT CGCATCAAAG
    CAGTTGCAGG TAGAAAGTTC ACAGCAAAGG CCCCAGCACA GAAAACTGAC ATCTCATCCA
    CGCTTCAAAA GAAGACACAA ATCCAGCGAA GATTTGCCTC GAACTGTCCC AATTAACAGC
    AAAGCCTCCC TGCCCTTCCA GCCTCCACCC CCAAGCCTGG ATGCTCTGGG CCCCATGGCT
    CAGCTGGCGC AGCTCCCGCT GGTGCCTCTG GACCCTGAGG AGCTGTGGGT GCACGAGAGC
    TGCATGGTGT GGACCAGTGG CATATACCTG GTCAATGGGA GACTCTATGG CCTTCAGGAA
    GCACTGGATG GTGCTAGAGA TGCGAGCTGC TCTCATTGTG AGATGGCGGG CTCCACCCTC
    GGCTGCTACA GTAAAGGCTG CACGCTGAGG TACCACTACA TTTGTGCCAT AGACGCAGAT
    TGCTCTCTTA ACGAAGACAA CTTTTCTTTG AGGTGTCCAA AGCACAAGGG CAACAGGATG
    GCCAAGCCTG GAGTGGTGTA TCTGGAGCAG TCGGAGCGCG GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa07773
    Clone ID Related Accession (Same CDS sequence) XM_021472464.1 , XM_005171536.3 , XM_009299007.2 , XM_005171536.4
    Accession Version XM_005171536.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product transcription factor 20
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171536.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCAGAATT TCCCCAACAG TCCTGTCCCT CTCCACCCAG GGTTCAGCCG TGGAGCAGGC 
    GTAGTCAGTT CCCCATATCA GACCCACCCG TCAGACCTTC AGATCTCCCC CAGGACCTCC
    GAGGACTATG TGTCCCTTCA GCAGGCCCAG ACCAGCCTAC AGAGCCACGG GCAGCACTTG
    CACCTGCGGG CCCAGCAGCA GCAGCACCTG CATGCTCCGG TACATGGCTA CAGCTCCAGG
    CGAGCGGTCA CAGAGATGCC GCAGAGCAGC GCGGGGGGGA ACTCCTACCG AAAGGACAGT
    ATGGATTATT ACTTCTCTGT GAGTGGGCGA GACCGAAGCC GAAGAGGTGG AGCCGCTTAC
    GGAGCCGGAT TTAGGTACAC AAACGTGGAT GGGCACCAGT ACCAACACCT CTCCGGTTCA
    GGGTCTTCCT CCGGAATGAT TTCTCCCTAC TCCATGGACT GTGGCTCTGC AGTCGTGGGG
    AGTAATAGCA GTGCAGCTGG TTCGTTTTCT CCTTCCCAGC AATACAGTAT GGCTCACGCT
    GCTTCAGTAC AATCAAGTGC TCAAATGCAT CAACGGCAGA AATATCCCTC ACATCAAGGC
    TTATTACACC AAGGTCAGCA TCACCGGACA TATGCGCTCT CCGGAAACAG AATCCCCGCT
    CAGTTCGGAC ACTACGCCAC TATCAATGCA ACCTCAGGCT CCTCTGGAAT GTATAACTCT
    CCACCGCAGA GATACGACGT GAGCAGCAGT AGCAGCACAG AAGCCAAAAT CAATGACTCT
    CCCACGCAGT CTAATCCAAA TCATCATAGC AGTTCATCAG CTTCCAACAG CTGCGAGGCT
    ATGGGGCAGG GCTACTCCTC GTCTGCACAT CCACCGTATT CACCACAGTC GCTTCACAAG
    CACGGCCCCC ATTCCCAGCG TACCCCTCAA CACAGCGCTG TCGCAGGCTA TGACCTATCC
    CTCAAAATGC AGCATCTGCA CACCAAACTG CCCCATTCCT CTGTCTCCTC CAGCCCGTCT
    CTGCCCTCAC ACACCCCTCA AGACCTCACC AAGTCTCCTA TGCACACTCA AAATCAACAG
    GCTCAGATTC ATCAGAACTT CAGTCCTATC TCTAATCCCT CCCCAGCTCC ATCTGCGGTG
    CAGTCTCCAA GCTGCAGCTC GTCCTCATCT CCACTGCTAG GGGTCTCGGA GGGAAACAGT
    GCTCAGCTGC AGGCGTCTTC ACACCCTGCT GCCCCAAACG CACGCAGCAG CCAGAGTCAC
    GCACGCCTCC TGCAGGCCGT CCCCCAGCTG AGCCCCACTC CCCATTCCAA TAGCAGCATC
    AGTAGCTGTG GTAGCGGAGG TGGAAATAAG TCTGCCACTC TGAATCACAG TGCAGGGATT
    GGTCATGCCA ATATGGGGCA GAACCGAATG GGGCCAGGTC TCCGGAGTGG TCCGGGGGAA
    GATAGCTCCC TGTTCCCTCA TGACAAACAC CTACCGGACC CTGGTCTAAA CAGTCTCAAT
    GCTCTGACTT CTCAAGTGGA AAACCTACCG AATACAGTCC AGCACATGCT GCTAACCGAC
    ACTGTGCTGC CCCAGAGGAA GAGCAGAGAC GCTGCCCCTC ACTTTCAGAT GCAGCAGGGG
    TCTCACTCTT TACCTGGCAA CCAGAACAAA TCTGGGAACT CCAGCACTGC TGGTCGGATC
    TTAACAAATG AAGAGAGCTC TGAGATGCTG GAAACAAAAG GCCAACAAAA ATTAGAAAGG
    AAAAGAGTTA GGCAGGAAAG TGGTACAAGC AATGAATCTG AGCCACAGAG CTACCTGCCA
    TCACAAAGGC AGATGCCCTC AGATAAGAGT CAGCAGGGCC TGGATCTCCA GGTCAACACT
    GGAGTCATCT CAACAGCTTC AAAACAGTCT TCTGTTAGAG AGTCTCAAGC AAAGACTCCA
    GAAACCCTCA CCCCCTCCTC CACTTCACCA CCATCAGGTC ATCCTACTGC AGAACCCAGC
    CCAAAGCTAC AGGTGTATCC CCCTGCTCCA GCCTCTCCCA TTCACTCCGC ATGTCTAAAC
    TCTGTGGCAG ATAGAGACCT AGGTAAAGAT GATGAGAAGG GAACTAAGGG ACCACAAATC
    AAAATGATAA AAGATGAAGA AAGCGATGAT GAATGTGCAG ATCCTCCGTG TCATAGAACG
    AGTTCAGAGA ACAGACTGTT GGTTCCTCTC AAACAGGCCG AGCGAGAGTC CTGTGCTCAA
    TCATTAGAAA ATCCCAGTAA AAATGTGAAT GTTCCAGAAC AACACAATGT TGGAGGTGTA
    GGGGTTATTG TCTCTGCTCG TTCTGAACTG AACACAGAAT CAAGCAAGCA CACAGGAGTC
    AAATCTCCAC GTTACAGTGT TTCTCATTAC CCAAACAAAC CCAGCAGTAT TGAGGAGCAG
    CATGATGTCA ATGTCTTAAA AGAGACGAGA AACCAGAATG GAGAAGGAGA GATGTCCATG
    GAAGCATATG TGTCACAGTA TGATATTTCC CCTAAACGAG AATTTGGCCA AAACATTGTT
    TCTGTTCAAT CTCACCCAGT GTCATTTAAA TGCAGCAATC CAGAGGTACA ATACACTGCC
    GTAAAGAGCA AGGACAAATT AGGGCTAGGT AGTGTTATGG GTGCAAACAG AGGCCAGAAT
    TACCATACAC TACAGGCCAG CTATGGGTCT ATTGCCAGAA AGGAGATTGC TGGTGTATTT
    GCTATGGAGG GGGGAAGAGG AATGGTCTCA AGAAGCCAGG ATAACAATTC TCAGTTTCAG
    CAGGTATTTC CAAGCCTTTT ACAAGAAGTC CTTCAAGGCC ATCACTTGGA TCGCCGCTAT
    GTACGTGCTG AACAGACATC TAGTGCTCAT CAACATTCTC AAGATACGTC TCAGCACCGT
    TTTCAAACCA GGCTACCTTA CAGTATGATT GAAAACTTGA GTTCACATTC AATGGGCTCT
    CAATCAGTTA TGGGGGGACT TAATGTCCAG TTTAGTCAAT TGGCCCCTGG AAAATCTCCA
    AACTCAAGTC AGGGACCAGA TAGTGAGATT GGTTTAGGCC CTTCTCATCC CTCTTGGGAT
    TCTGAAGCAC ATAAGACTCA CGGGATCTCC ATAGAGAAAA GCAAAACTAG TCTGTCTCCC
    AGCCAGTCCT CCCACATGCA GCAGTCTTTA GATCTCTCAA CAGGAGCCCC TCCAAAGCAC
    ATCAACCTGG CTGACTATTC TTTGCAGCAC AGAAAACCAT CCAGATATGG TACCTCTCCT
    TCAGCTGTAG AACAATTGCT TTTACAGGAA GCTGAGCCAC TGGCATGCAA TGTAGGTCCT
    GTCGGCCATG TTCAATCCCA GGCATCCTCA TCCTCTGGAA GGCGCTCGGT AATCTGTGAT
    GTGTCGCCTT CTCGACGAAC AACACCAGAA AGAGAGAGAG GATCCTCTGG ACCCTCAGTC
    ATTCAGCAGC CGTTTTCTTC TGGGTCTAAT GAGCAAGACT GCTGCAAGGA AGAAATTAAA
    GCAAAGAAAG CACAAATCAA GGAGGACCCA TCAAAGGTTG AAGCTGCAGG TCAGAACACA
    GATCGCTACT GCAGCACACC ACCCAGTAAG GAGTCTAATA AGCCTCTTCG CCATCCTGTA
    GATAGTGATC CTGACTTAGA AAAGACCAGC AGTGCCAAGG GCAACAGTGA TGCCCTCAGC
    AACCTGCCAT ATCTTTCACA AATGCCCACA AACCCCTTGT CCTCACCACA GAGACTCCAG
    TCATTTTCAC AGGCTGTTGA TGGCTTAAGG GCATATGGCT TCGGTGATGC AATGGATGGA
    CAAAAAATGA TCCCTCATGC TGGACCACAT CAACCGTTCC ATACGCTATC AGCATATACT
    AAGACCACAG CCTCTACAAA CAAGTTACAA GTGTTTCCAC AGAGTTTGCC TCCTCAAGAC
    AGACCTGACT GGACTCCTGA CAGACAAAAG CTTCGAGGTA TGGACAGGCA CGGTCCTCAG
    AGAATCCCCG AACAGAAATG TAAAAGCCAG CCTTCAAGTG AAATTCTGTC TAGTCCACAT
    CGTATATCTC AACAGCACTC TTATCCTGGT GCACAGTTTG ACCTGAAAAT GTGGGATGCT
    TATCCTGAAA AAGAGGGAGC TGCGCAACCA CTGGCTGCAG TCCCTCGTGA GCATGTTGGT
    TCTCAACCAA TTACAAATTC TGGCCCCAAA ATTGCAAAAG AGGACATCTC AGATAAGGGT
    GCAGAAGACA CAGTCAAATC CTTCCACTCA TTGGCACCGG CTAGTACTAA TGCTGGGCAC
    ACCGTCAAGG CAACCCAAGG CATTCAGCAG GGGCAGAGAG TAACTAAAAC TGGAGCATTG
    GCTGAAACTA ATCCCCTAAT CATGAGAAGG AGAGTTAGAT CTTTTATTTC CCCTATTCCT
    GCTAAGAGGC AGCATCAGGA TGTTCCAGGT CAGCGGCCAG GATCAGCATA CCACTCCCCA
    ATAGCTCAAG CCTACCCTGA ATCTAGAATT GCAACCAACA GTTGTTCTGG TAGTTCGGAT
    TCTCAGGCCA AATTACCATC TCCCAAGACA CCGCATTCCA TATCTAGTGC TAACTCGCCT
    CCTCAGTCCA AAGCAAAGTT TCTTCCCCCA AGAAAGGGGC GTGGCTTGAA ACTTGAGGCG
    ATTGTGCAGA AAATTACTCC TGGCGTGAGA AAAAATGACT TCAACAATAG TAACTTGGAA
    TCGGACTTTT CAGAAGCAGC TCATTACACC TCTGACTTGC CGGACCCAGA GAGTGGTACA
    TCGTTTACTA GTGTTTCTCA AGGTGAGGAG ACCTGTTTAT CTTACCTTGA GGATTCTCAT
    ACTTTAGATG AGCTTATGCC ATACAGAGCA GTTGAAGATG CATTCTCATG TGACTCTCAG
    TCTCTCAAAG CAGGTGCAGC TGCTTCTAGC AGCGGTGCCC TCAGAAGTTT GCCAAAAGAT
    TTCGACTTTG GCTTAGGTAC TGCTGGCTCT TCAGGGTCTT TGGGGGGTGA AAATGATAAG
    GATGAATTTA CTTTGTTGGG ACCCCTTCCA CCAGCTCCTC CATTGCCTTG TCCTGTACAG
    GGATCACCTC CTCCATCTTC ATCTGCATTG TCAGACATAC AACAATTCAC AAACACTTAT
    CAACAGCTGG AAACAAGGCG AAGTGAGCAG TCGGCTGCTA ACCTGCTGAG GCAGAAGTTG
    CAGGAGACTG GCATGGGCTT TGACGATTAC CCAGGAGGGG ATTACTATGG GACCACTGCT
    GGCCATAGTC CTGGACATCA CCTACTGTCC CGAGGTTCAC AAAACCAGAT GACTTCTCTC
    AGGTTGACAA GTCCAGACTC TAAAGCATCA GAAAGCACTG TGCCCAAAGG TTATTTCCCA
    TCAGGAAAGA AAAAAGGCCG CCCAGTCGGT AGTGTGAATA AGCAGAAACG TGCCCAAGGA
    CAAATGCAGA ATACAGCAAC TAACATACCT GCAGCTCCTC TTCCCTCACC TACAGCTGCA
    CCTCAGTCTG TTTCAGTCTC AAGCAATGCT GATGAAGTGC CCCGAGTACC AACACCACCT
    GATGAGAAAC CCTGCCCATC TCTGGTCCCT CCCGATGAAA CCCAAGTGGT GAAGGTGGAT
    GCAGAAAGCG AGGAGACCCT GCCAGAGTTG GATGTAAAGC CTGTTAAACA AAAACAGAAA
    AAGGGGAAAG AGTGCAGCGA GACTCCTGCC TCAAGTGGCA ATCAGAGAAG GAGAAGAAGA
    GGAATGGCTT CCAAAGAGGA GCTGGACACT CAGACGAGCA GTAGGGGAAG CATTAGTCTC
    AGTGGAATAT TCTCTGATGC CAGGAATAGT GTTTTTGCTC CTTACGTACA TGTGGAGAAA
    AAGATGGAAG AAATTGGGGC AGTCTGTACA ATAATTAACG CAGAGGATGA AAAGTCAAAA
    GGTGGAGGCA AACCTGGTCA CACGGGGGTT GATGGCCCTT TAAACACCCT GCTGCCATCT
    CAGCTTGTGA GAAAAGAAAA GGAGAATGAA AAATCAAAAG AGAACTGGGT TTCAGAGCAA
    GTTGATTCAA CCTTGGAGTC AGGGAAAGCA CTTCCTACAT CCGAGTATGT TCTGCCAGGG
    CCTGTGATAT CCGAAACTGG ACACAGCGGT CACCTGTTGT GCTGCCTTTG TCAGAAATGG
    GCCAACTACA AACATCTTGG AGATCTCTAT GGTCCTTTTT ACCCAGCCGA GTATGCAGCC
    AAGCTCCCTA AGAATCAACC TCAAGTCCGA CAAACTTTGT CCCATTTTGG GCCAGCTGCC
    ACAGGTATGA CATCCATTCC AACAGAAGCG ATTCCCCAGG ATACAAGATT ACAAGAACCT
    CAGAATGTCA AGTCATCTGC AGAAAGCGAT TGCACAGTAA GCCAGGCAAC AAACACAACA
    TCCCCAGCCA CTATCATTGG CACTGTTTCT CCAATGCCTT TGGAAATGCC TTTCCAGAAT
    GCTATAGCCA GCAGCTCTAC TGCAAGGGTA ACAACTCATG CTTGGGATAC GGCAGCCGAG
    CTGGAAAGTG GACTGGGTAC ATCAAATTTA CAAGAAACAG ACGCTGAAAT CGCATCAAAG
    CAGTTGCAGG TAGAAAGTTC ACAGCAAAGG CCCCAGCACA GAAAACTGAC ATCTCATCCA
    CGCTTCAAAA GAAGACACAA ATCCAGCGAA GATTTGCCTC GAACTGTCCC AATTAACAGC
    AAAGCCTCCC TGCCCTTCCA GCCTCCACCC CCAAGCCTGG ATGCTCTGGG CCCCATGGCT
    CAGCTGGCGC AGCTCCCGCT GGTGCCTCTG GACCCTGAGG AGCTGTGGGT GCACGAGAGC
    TGCATGGTGT GGACCAGTGG CATATACCTG GTCAATGGGA GACTCTATGG CCTTCAGGAA
    GCACTGGATG GTGCTAGAGA TGCGAGCTGC TCTCATTGTG AGATGGCGGG CTCCACCCTC
    GGCTGCTACA GTAAAGGCTG CACGCTGAGG TACCACTACA TTTGTGCCAT AGACGCAGAT
    TGCTCTCTTA ACGAAGACAA CTTTTCTTTG AGGTGTCCAA AGCACAAGGG CAACAGGATG
    GCCAAGCCTG GAGTGGTGTA TCTGGAGCAG TCGGAGCGCG GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005171593.1
    CDS1066..8130
    Translation

    Target ORF information:

    RefSeq Version XM_005171536.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio transcription factor 20 (tcf20), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005171536.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCAGAATT TCCCCAACAG TCCTGTCCCT CTCCACCCAG GGTTCAGCCG TGGAGCAGGC 
    GTAGTCAGTT CCCCATATCA GACCCACCCG TCAGACCTTC AGATCTCCCC CAGGACCTCC
    GAGGACTATG TGTCCCTTCA GCAGGCCCAG ACCAGCCTAC AGAGCCACGG GCAGCACTTG
    CACCTGCGGG CCCAGCAGCA GCAGCACCTG CATGCTCCGG TACATGGCTA CAGCTCCAGG
    CGAGCGGTCA CAGAGATGCC GCAGAGCAGC GCGGGGGGGA ACTCCTACCG AAAGGACAGT
    ATGGATTATT ACTTCTCTGT GAGTGGGCGA GACCGAAGCC GAAGAGGTGG AGCCGCTTAC
    GGAGCCGGAT TTAGGTACAC AAACGTGGAT GGGCACCAGT ACCAACACCT CTCCGGTTCA
    GGGTCTTCCT CCGGAATGAT TTCTCCCTAC TCCATGGACT GTGGCTCTGC AGTCGTGGGG
    AGTAATAGCA GTGCAGCTGG TTCGTTTTCT CCTTCCCAGC AATACAGTAT GGCTCACGCT
    GCTTCAGTAC AATCAAGTGC TCAAATGCAT CAACGGCAGA AATATCCCTC ACATCAAGGC
    TTATTACACC AAGGTCAGCA TCACCGGACA TATGCGCTCT CCGGAAACAG AATCCCCGCT
    CAGTTCGGAC ACTACGCCAC TATCAATGCA ACCTCAGGCT CCTCTGGAAT GTATAACTCT
    CCACCGCAGA GATACGACGT GAGCAGCAGT AGCAGCACAG AAGCCAAAAT CAATGACTCT
    CCCACGCAGT CTAATCCAAA TCATCATAGC AGTTCATCAG CTTCCAACAG CTGCGAGGCT
    ATGGGGCAGG GCTACTCCTC GTCTGCACAT CCACCGTATT CACCACAGTC GCTTCACAAG
    CACGGCCCCC ATTCCCAGCG TACCCCTCAA CACAGCGCTG TCGCAGGCTA TGACCTATCC
    CTCAAAATGC AGCATCTGCA CACCAAACTG CCCCATTCCT CTGTCTCCTC CAGCCCGTCT
    CTGCCCTCAC ACACCCCTCA AGACCTCACC AAGTCTCCTA TGCACACTCA AAATCAACAG
    GCTCAGATTC ATCAGAACTT CAGTCCTATC TCTAATCCCT CCCCAGCTCC ATCTGCGGTG
    CAGTCTCCAA GCTGCAGCTC GTCCTCATCT CCACTGCTAG GGGTCTCGGA GGGAAACAGT
    GCTCAGCTGC AGGCGTCTTC ACACCCTGCT GCCCCAAACG CACGCAGCAG CCAGAGTCAC
    GCACGCCTCC TGCAGGCCGT CCCCCAGCTG AGCCCCACTC CCCATTCCAA TAGCAGCATC
    AGTAGCTGTG GTAGCGGAGG TGGAAATAAG TCTGCCACTC TGAATCACAG TGCAGGGATT
    GGTCATGCCA ATATGGGGCA GAACCGAATG GGGCCAGGTC TCCGGAGTGG TCCGGGGGAA
    GATAGCTCCC TGTTCCCTCA TGACAAACAC CTACCGGACC CTGGTCTAAA CAGTCTCAAT
    GCTCTGACTT CTCAAGTGGA AAACCTACCG AATACAGTCC AGCACATGCT GCTAACCGAC
    ACTGTGCTGC CCCAGAGGAA GAGCAGAGAC GCTGCCCCTC ACTTTCAGAT GCAGCAGGGG
    TCTCACTCTT TACCTGGCAA CCAGAACAAA TCTGGGAACT CCAGCACTGC TGGTCGGATC
    TTAACAAATG AAGAGAGCTC TGAGATGCTG GAAACAAAAG GCCAACAAAA ATTAGAAAGG
    AAAAGAGTTA GGCAGGAAAG TGGTACAAGC AATGAATCTG AGCCACAGAG CTACCTGCCA
    TCACAAAGGC AGATGCCCTC AGATAAGAGT CAGCAGGGCC TGGATCTCCA GGTCAACACT
    GGAGTCATCT CAACAGCTTC AAAACAGTCT TCTGTTAGAG AGTCTCAAGC AAAGACTCCA
    GAAACCCTCA CCCCCTCCTC CACTTCACCA CCATCAGGTC ATCCTACTGC AGAACCCAGC
    CCAAAGCTAC AGGTGTATCC CCCTGCTCCA GCCTCTCCCA TTCACTCCGC ATGTCTAAAC
    TCTGTGGCAG ATAGAGACCT AGGTAAAGAT GATGAGAAGG GAACTAAGGG ACCACAAATC
    AAAATGATAA AAGATGAAGA AAGCGATGAT GAATGTGCAG ATCCTCCGTG TCATAGAACG
    AGTTCAGAGA ACAGACTGTT GGTTCCTCTC AAACAGGCCG AGCGAGAGTC CTGTGCTCAA
    TCATTAGAAA ATCCCAGTAA AAATGTGAAT GTTCCAGAAC AACACAATGT TGGAGGTGTA
    GGGGTTATTG TCTCTGCTCG TTCTGAACTG AACACAGAAT CAAGCAAGCA CACAGGAGTC
    AAATCTCCAC GTTACAGTGT TTCTCATTAC CCAAACAAAC CCAGCAGTAT TGAGGAGCAG
    CATGATGTCA ATGTCTTAAA AGAGACGAGA AACCAGAATG GAGAAGGAGA GATGTCCATG
    GAAGCATATG TGTCACAGTA TGATATTTCC CCTAAACGAG AATTTGGCCA AAACATTGTT
    TCTGTTCAAT CTCACCCAGT GTCATTTAAA TGCAGCAATC CAGAGGTACA ATACACTGCC
    GTAAAGAGCA AGGACAAATT AGGGCTAGGT AGTGTTATGG GTGCAAACAG AGGCCAGAAT
    TACCATACAC TACAGGCCAG CTATGGGTCT ATTGCCAGAA AGGAGATTGC TGGTGTATTT
    GCTATGGAGG GGGGAAGAGG AATGGTCTCA AGAAGCCAGG ATAACAATTC TCAGTTTCAG
    CAGGTATTTC CAAGCCTTTT ACAAGAAGTC CTTCAAGGCC ATCACTTGGA TCGCCGCTAT
    GTACGTGCTG AACAGACATC TAGTGCTCAT CAACATTCTC AAGATACGTC TCAGCACCGT
    TTTCAAACCA GGCTACCTTA CAGTATGATT GAAAACTTGA GTTCACATTC AATGGGCTCT
    CAATCAGTTA TGGGGGGACT TAATGTCCAG TTTAGTCAAT TGGCCCCTGG AAAATCTCCA
    AACTCAAGTC AGGGACCAGA TAGTGAGATT GGTTTAGGCC CTTCTCATCC CTCTTGGGAT
    TCTGAAGCAC ATAAGACTCA CGGGATCTCC ATAGAGAAAA GCAAAACTAG TCTGTCTCCC
    AGCCAGTCCT CCCACATGCA GCAGTCTTTA GATCTCTCAA CAGGAGCCCC TCCAAAGCAC
    ATCAACCTGG CTGACTATTC TTTGCAGCAC AGAAAACCAT CCAGATATGG TACCTCTCCT
    TCAGCTGTAG AACAATTGCT TTTACAGGAA GCTGAGCCAC TGGCATGCAA TGTAGGTCCT
    GTCGGCCATG TTCAATCCCA GGCATCCTCA TCCTCTGGAA GGCGCTCGGT AATCTGTGAT
    GTGTCGCCTT CTCGACGAAC AACACCAGAA AGAGAGAGAG GATCCTCTGG ACCCTCAGTC
    ATTCAGCAGC CGTTTTCTTC TGGGTCTAAT GAGCAAGACT GCTGCAAGGA AGAAATTAAA
    GCAAAGAAAG CACAAATCAA GGAGGACCCA TCAAAGGTTG AAGCTGCAGG TCAGAACACA
    GATCGCTACT GCAGCACACC ACCCAGTAAG GAGTCTAATA AGCCTCTTCG CCATCCTGTA
    GATAGTGATC CTGACTTAGA AAAGACCAGC AGTGCCAAGG GCAACAGTGA TGCCCTCAGC
    AACCTGCCAT ATCTTTCACA AATGCCCACA AACCCCTTGT CCTCACCACA GAGACTCCAG
    TCATTTTCAC AGGCTGTTGA TGGCTTAAGG GCATATGGCT TCGGTGATGC AATGGATGGA
    CAAAAAATGA TCCCTCATGC TGGACCACAT CAACCGTTCC ATACGCTATC AGCATATACT
    AAGACCACAG CCTCTACAAA CAAGTTACAA GTGTTTCCAC AGAGTTTGCC TCCTCAAGAC
    AGACCTGACT GGACTCCTGA CAGACAAAAG CTTCGAGGTA TGGACAGGCA CGGTCCTCAG
    AGAATCCCCG AACAGAAATG TAAAAGCCAG CCTTCAAGTG AAATTCTGTC TAGTCCACAT
    CGTATATCTC AACAGCACTC TTATCCTGGT GCACAGTTTG ACCTGAAAAT GTGGGATGCT
    TATCCTGAAA AAGAGGGAGC TGCGCAACCA CTGGCTGCAG TCCCTCGTGA GCATGTTGGT
    TCTCAACCAA TTACAAATTC TGGCCCCAAA ATTGCAAAAG AGGACATCTC AGATAAGGGT
    GCAGAAGACA CAGTCAAATC CTTCCACTCA TTGGCACCGG CTAGTACTAA TGCTGGGCAC
    ACCGTCAAGG CAACCCAAGG CATTCAGCAG GGGCAGAGAG TAACTAAAAC TGGAGCATTG
    GCTGAAACTA ATCCCCTAAT CATGAGAAGG AGAGTTAGAT CTTTTATTTC CCCTATTCCT
    GCTAAGAGGC AGCATCAGGA TGTTCCAGGT CAGCGGCCAG GATCAGCATA CCACTCCCCA
    ATAGCTCAAG CCTACCCTGA ATCTAGAATT GCAACCAACA GTTGTTCTGG TAGTTCGGAT
    TCTCAGGCCA AATTACCATC TCCCAAGACA CCGCATTCCA TATCTAGTGC TAACTCGCCT
    CCTCAGTCCA AAGCAAAGTT TCTTCCCCCA AGAAAGGGGC GTGGCTTGAA ACTTGAGGCG
    ATTGTGCAGA AAATTACTCC TGGCGTGAGA AAAAATGACT TCAACAATAG TAACTTGGAA
    TCGGACTTTT CAGAAGCAGC TCATTACACC TCTGACTTGC CGGACCCAGA GAGTGGTACA
    TCGTTTACTA GTGTTTCTCA AGGTGAGGAG ACCTGTTTAT CTTACCTTGA GGATTCTCAT
    ACTTTAGATG AGCTTATGCC ATACAGAGCA GTTGAAGATG CATTCTCATG TGACTCTCAG
    TCTCTCAAAG CAGGTGCAGC TGCTTCTAGC AGCGGTGCCC TCAGAAGTTT GCCAAAAGAT
    TTCGACTTTG GCTTAGGTAC TGCTGGCTCT TCAGGGTCTT TGGGGGGTGA AAATGATAAG
    GATGAATTTA CTTTGTTGGG ACCCCTTCCA CCAGCTCCTC CATTGCCTTG TCCTGTACAG
    GGATCACCTC CTCCATCTTC ATCTGCATTG TCAGACATAC AACAATTCAC AAACACTTAT
    CAACAGCTGG AAACAAGGCG AAGTGAGCAG TCGGCTGCTA ACCTGCTGAG GCAGAAGTTG
    CAGGAGACTG GCATGGGCTT TGACGATTAC CCAGGAGGGG ATTACTATGG GACCACTGCT
    GGCCATAGTC CTGGACATCA CCTACTGTCC CGAGGTTCAC AAAACCAGAT GACTTCTCTC
    AGGTTGACAA GTCCAGACTC TAAAGCATCA GAAAGCACTG TGCCCAAAGG TTATTTCCCA
    TCAGGAAAGA AAAAAGGCCG CCCAGTCGGT AGTGTGAATA AGCAGAAACG TGCCCAAGGA
    CAAATGCAGA ATACAGCAAC TAACATACCT GCAGCTCCTC TTCCCTCACC TACAGCTGCA
    CCTCAGTCTG TTTCAGTCTC AAGCAATGCT GATGAAGTGC CCCGAGTACC AACACCACCT
    GATGAGAAAC CCTGCCCATC TCTGGTCCCT CCCGATGAAA CCCAAGTGGT GAAGGTGGAT
    GCAGAAAGCG AGGAGACCCT GCCAGAGTTG GATGTAAAGC CTGTTAAACA AAAACAGAAA
    AAGGGGAAAG AGTGCAGCGA GACTCCTGCC TCAAGTGGCA ATCAGAGAAG GAGAAGAAGA
    GGAATGGCTT CCAAAGAGGA GCTGGACACT CAGACGAGCA GTAGGGGAAG CATTAGTCTC
    AGTGGAATAT TCTCTGATGC CAGGAATAGT GTTTTTGCTC CTTACGTACA TGTGGAGAAA
    AAGATGGAAG AAATTGGGGC AGTCTGTACA ATAATTAACG CAGAGGATGA AAAGTCAAAA
    GGTGGAGGCA AACCTGGTCA CACGGGGGTT GATGGCCCTT TAAACACCCT GCTGCCATCT
    CAGCTTGTGA GAAAAGAAAA GGAGAATGAA AAATCAAAAG AGAACTGGGT TTCAGAGCAA
    GTTGATTCAA CCTTGGAGTC AGGGAAAGCA CTTCCTACAT CCGAGTATGT TCTGCCAGGG
    CCTGTGATAT CCGAAACTGG ACACAGCGGT CACCTGTTGT GCTGCCTTTG TCAGAAATGG
    GCCAACTACA AACATCTTGG AGATCTCTAT GGTCCTTTTT ACCCAGCCGA GTATGCAGCC
    AAGCTCCCTA AGAATCAACC TCAAGTCCGA CAAACTTTGT CCCATTTTGG GCCAGCTGCC
    ACAGGTATGA CATCCATTCC AACAGAAGCG ATTCCCCAGG ATACAAGATT ACAAGAACCT
    CAGAATGTCA AGTCATCTGC AGAAAGCGAT TGCACAGTAA GCCAGGCAAC AAACACAACA
    TCCCCAGCCA CTATCATTGG CACTGTTTCT CCAATGCCTT TGGAAATGCC TTTCCAGAAT
    GCTATAGCCA GCAGCTCTAC TGCAAGGGTA ACAACTCATG CTTGGGATAC GGCAGCCGAG
    CTGGAAAGTG GACTGGGTAC ATCAAATTTA CAAGAAACAG ACGCTGAAAT CGCATCAAAG
    CAGTTGCAGG TAGAAAGTTC ACAGCAAAGG CCCCAGCACA GAAAACTGAC ATCTCATCCA
    CGCTTCAAAA GAAGACACAA ATCCAGCGAA GATTTGCCTC GAACTGTCCC AATTAACAGC
    AAAGCCTCCC TGCCCTTCCA GCCTCCACCC CCAAGCCTGG ATGCTCTGGG CCCCATGGCT
    CAGCTGGCGC AGCTCCCGCT GGTGCCTCTG GACCCTGAGG AGCTGTGGGT GCACGAGAGC
    TGCATGGTGT GGACCAGTGG CATATACCTG GTCAATGGGA GACTCTATGG CCTTCAGGAA
    GCACTGGATG GTGCTAGAGA TGCGAGCTGC TCTCATTGTG AGATGGCGGG CTCCACCCTC
    GGCTGCTACA GTAAAGGCTG CACGCTGAGG TACCACTACA TTTGTGCCAT AGACGCAGAT
    TGCTCTCTTA ACGAAGACAA CTTTTCTTTG AGGTGTCCAA AGCACAAGGG CAACAGGATG
    GCCAAGCCTG GAGTGGTGTA TCTGGAGCAG TCGGAGCGCG GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa07773
    Clone ID Related Accession (Same CDS sequence) XM_021472464.1 , XM_005171536.3 , XM_009299007.2 , XM_005171536.4
    Accession Version XM_009299007.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product transcription factor 20
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299007.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCAGAATT TCCCCAACAG TCCTGTCCCT CTCCACCCAG GGTTCAGCCG TGGAGCAGGC 
    GTAGTCAGTT CCCCATATCA GACCCACCCG TCAGACCTTC AGATCTCCCC CAGGACCTCC
    GAGGACTATG TGTCCCTTCA GCAGGCCCAG ACCAGCCTAC AGAGCCACGG GCAGCACTTG
    CACCTGCGGG CCCAGCAGCA GCAGCACCTG CATGCTCCGG TACATGGCTA CAGCTCCAGG
    CGAGCGGTCA CAGAGATGCC GCAGAGCAGC GCGGGGGGGA ACTCCTACCG AAAGGACAGT
    ATGGATTATT ACTTCTCTGT GAGTGGGCGA GACCGAAGCC GAAGAGGTGG AGCCGCTTAC
    GGAGCCGGAT TTAGGTACAC AAACGTGGAT GGGCACCAGT ACCAACACCT CTCCGGTTCA
    GGGTCTTCCT CCGGAATGAT TTCTCCCTAC TCCATGGACT GTGGCTCTGC AGTCGTGGGG
    AGTAATAGCA GTGCAGCTGG TTCGTTTTCT CCTTCCCAGC AATACAGTAT GGCTCACGCT
    GCTTCAGTAC AATCAAGTGC TCAAATGCAT CAACGGCAGA AATATCCCTC ACATCAAGGC
    TTATTACACC AAGGTCAGCA TCACCGGACA TATGCGCTCT CCGGAAACAG AATCCCCGCT
    CAGTTCGGAC ACTACGCCAC TATCAATGCA ACCTCAGGCT CCTCTGGAAT GTATAACTCT
    CCACCGCAGA GATACGACGT GAGCAGCAGT AGCAGCACAG AAGCCAAAAT CAATGACTCT
    CCCACGCAGT CTAATCCAAA TCATCATAGC AGTTCATCAG CTTCCAACAG CTGCGAGGCT
    ATGGGGCAGG GCTACTCCTC GTCTGCACAT CCACCGTATT CACCACAGTC GCTTCACAAG
    CACGGCCCCC ATTCCCAGCG TACCCCTCAA CACAGCGCTG TCGCAGGCTA TGACCTATCC
    CTCAAAATGC AGCATCTGCA CACCAAACTG CCCCATTCCT CTGTCTCCTC CAGCCCGTCT
    CTGCCCTCAC ACACCCCTCA AGACCTCACC AAGTCTCCTA TGCACACTCA AAATCAACAG
    GCTCAGATTC ATCAGAACTT CAGTCCTATC TCTAATCCCT CCCCAGCTCC ATCTGCGGTG
    CAGTCTCCAA GCTGCAGCTC GTCCTCATCT CCACTGCTAG GGGTCTCGGA GGGAAACAGT
    GCTCAGCTGC AGGCGTCTTC ACACCCTGCT GCCCCAAACG CACGCAGCAG CCAGAGTCAC
    GCACGCCTCC TGCAGGCCGT CCCCCAGCTG AGCCCCACTC CCCATTCCAA TAGCAGCATC
    AGTAGCTGTG GTAGCGGAGG TGGAAATAAG TCTGCCACTC TGAATCACAG TGCAGGGATT
    GGTCATGCCA ATATGGGGCA GAACCGAATG GGGCCAGGTC TCCGGAGTGG TCCGGGGGAA
    GATAGCTCCC TGTTCCCTCA TGACAAACAC CTACCGGACC CTGGTCTAAA CAGTCTCAAT
    GCTCTGACTT CTCAAGTGGA AAACCTACCG AATACAGTCC AGCACATGCT GCTAACCGAC
    ACTGTGCTGC CCCAGAGGAA GAGCAGAGAC GCTGCCCCTC ACTTTCAGAT GCAGCAGGGG
    TCTCACTCTT TACCTGGCAA CCAGAACAAA TCTGGGAACT CCAGCACTGC TGGTCGGATC
    TTAACAAATG AAGAGAGCTC TGAGATGCTG GAAACAAAAG GCCAACAAAA ATTAGAAAGG
    AAAAGAGTTA GGCAGGAAAG TGGTACAAGC AATGAATCTG AGCCACAGAG CTACCTGCCA
    TCACAAAGGC AGATGCCCTC AGATAAGAGT CAGCAGGGCC TGGATCTCCA GGTCAACACT
    GGAGTCATCT CAACAGCTTC AAAACAGTCT TCTGTTAGAG AGTCTCAAGC AAAGACTCCA
    GAAACCCTCA CCCCCTCCTC CACTTCACCA CCATCAGGTC ATCCTACTGC AGAACCCAGC
    CCAAAGCTAC AGGTGTATCC CCCTGCTCCA GCCTCTCCCA TTCACTCCGC ATGTCTAAAC
    TCTGTGGCAG ATAGAGACCT AGGTAAAGAT GATGAGAAGG GAACTAAGGG ACCACAAATC
    AAAATGATAA AAGATGAAGA AAGCGATGAT GAATGTGCAG ATCCTCCGTG TCATAGAACG
    AGTTCAGAGA ACAGACTGTT GGTTCCTCTC AAACAGGCCG AGCGAGAGTC CTGTGCTCAA
    TCATTAGAAA ATCCCAGTAA AAATGTGAAT GTTCCAGAAC AACACAATGT TGGAGGTGTA
    GGGGTTATTG TCTCTGCTCG TTCTGAACTG AACACAGAAT CAAGCAAGCA CACAGGAGTC
    AAATCTCCAC GTTACAGTGT TTCTCATTAC CCAAACAAAC CCAGCAGTAT TGAGGAGCAG
    CATGATGTCA ATGTCTTAAA AGAGACGAGA AACCAGAATG GAGAAGGAGA GATGTCCATG
    GAAGCATATG TGTCACAGTA TGATATTTCC CCTAAACGAG AATTTGGCCA AAACATTGTT
    TCTGTTCAAT CTCACCCAGT GTCATTTAAA TGCAGCAATC CAGAGGTACA ATACACTGCC
    GTAAAGAGCA AGGACAAATT AGGGCTAGGT AGTGTTATGG GTGCAAACAG AGGCCAGAAT
    TACCATACAC TACAGGCCAG CTATGGGTCT ATTGCCAGAA AGGAGATTGC TGGTGTATTT
    GCTATGGAGG GGGGAAGAGG AATGGTCTCA AGAAGCCAGG ATAACAATTC TCAGTTTCAG
    CAGGTATTTC CAAGCCTTTT ACAAGAAGTC CTTCAAGGCC ATCACTTGGA TCGCCGCTAT
    GTACGTGCTG AACAGACATC TAGTGCTCAT CAACATTCTC AAGATACGTC TCAGCACCGT
    TTTCAAACCA GGCTACCTTA CAGTATGATT GAAAACTTGA GTTCACATTC AATGGGCTCT
    CAATCAGTTA TGGGGGGACT TAATGTCCAG TTTAGTCAAT TGGCCCCTGG AAAATCTCCA
    AACTCAAGTC AGGGACCAGA TAGTGAGATT GGTTTAGGCC CTTCTCATCC CTCTTGGGAT
    TCTGAAGCAC ATAAGACTCA CGGGATCTCC ATAGAGAAAA GCAAAACTAG TCTGTCTCCC
    AGCCAGTCCT CCCACATGCA GCAGTCTTTA GATCTCTCAA CAGGAGCCCC TCCAAAGCAC
    ATCAACCTGG CTGACTATTC TTTGCAGCAC AGAAAACCAT CCAGATATGG TACCTCTCCT
    TCAGCTGTAG AACAATTGCT TTTACAGGAA GCTGAGCCAC TGGCATGCAA TGTAGGTCCT
    GTCGGCCATG TTCAATCCCA GGCATCCTCA TCCTCTGGAA GGCGCTCGGT AATCTGTGAT
    GTGTCGCCTT CTCGACGAAC AACACCAGAA AGAGAGAGAG GATCCTCTGG ACCCTCAGTC
    ATTCAGCAGC CGTTTTCTTC TGGGTCTAAT GAGCAAGACT GCTGCAAGGA AGAAATTAAA
    GCAAAGAAAG CACAAATCAA GGAGGACCCA TCAAAGGTTG AAGCTGCAGG TCAGAACACA
    GATCGCTACT GCAGCACACC ACCCAGTAAG GAGTCTAATA AGCCTCTTCG CCATCCTGTA
    GATAGTGATC CTGACTTAGA AAAGACCAGC AGTGCCAAGG GCAACAGTGA TGCCCTCAGC
    AACCTGCCAT ATCTTTCACA AATGCCCACA AACCCCTTGT CCTCACCACA GAGACTCCAG
    TCATTTTCAC AGGCTGTTGA TGGCTTAAGG GCATATGGCT TCGGTGATGC AATGGATGGA
    CAAAAAATGA TCCCTCATGC TGGACCACAT CAACCGTTCC ATACGCTATC AGCATATACT
    AAGACCACAG CCTCTACAAA CAAGTTACAA GTGTTTCCAC AGAGTTTGCC TCCTCAAGAC
    AGACCTGACT GGACTCCTGA CAGACAAAAG CTTCGAGGTA TGGACAGGCA CGGTCCTCAG
    AGAATCCCCG AACAGAAATG TAAAAGCCAG CCTTCAAGTG AAATTCTGTC TAGTCCACAT
    CGTATATCTC AACAGCACTC TTATCCTGGT GCACAGTTTG ACCTGAAAAT GTGGGATGCT
    TATCCTGAAA AAGAGGGAGC TGCGCAACCA CTGGCTGCAG TCCCTCGTGA GCATGTTGGT
    TCTCAACCAA TTACAAATTC TGGCCCCAAA ATTGCAAAAG AGGACATCTC AGATAAGGGT
    GCAGAAGACA CAGTCAAATC CTTCCACTCA TTGGCACCGG CTAGTACTAA TGCTGGGCAC
    ACCGTCAAGG CAACCCAAGG CATTCAGCAG GGGCAGAGAG TAACTAAAAC TGGAGCATTG
    GCTGAAACTA ATCCCCTAAT CATGAGAAGG AGAGTTAGAT CTTTTATTTC CCCTATTCCT
    GCTAAGAGGC AGCATCAGGA TGTTCCAGGT CAGCGGCCAG GATCAGCATA CCACTCCCCA
    ATAGCTCAAG CCTACCCTGA ATCTAGAATT GCAACCAACA GTTGTTCTGG TAGTTCGGAT
    TCTCAGGCCA AATTACCATC TCCCAAGACA CCGCATTCCA TATCTAGTGC TAACTCGCCT
    CCTCAGTCCA AAGCAAAGTT TCTTCCCCCA AGAAAGGGGC GTGGCTTGAA ACTTGAGGCG
    ATTGTGCAGA AAATTACTCC TGGCGTGAGA AAAAATGACT TCAACAATAG TAACTTGGAA
    TCGGACTTTT CAGAAGCAGC TCATTACACC TCTGACTTGC CGGACCCAGA GAGTGGTACA
    TCGTTTACTA GTGTTTCTCA AGGTGAGGAG ACCTGTTTAT CTTACCTTGA GGATTCTCAT
    ACTTTAGATG AGCTTATGCC ATACAGAGCA GTTGAAGATG CATTCTCATG TGACTCTCAG
    TCTCTCAAAG CAGGTGCAGC TGCTTCTAGC AGCGGTGCCC TCAGAAGTTT GCCAAAAGAT
    TTCGACTTTG GCTTAGGTAC TGCTGGCTCT TCAGGGTCTT TGGGGGGTGA AAATGATAAG
    GATGAATTTA CTTTGTTGGG ACCCCTTCCA CCAGCTCCTC CATTGCCTTG TCCTGTACAG
    GGATCACCTC CTCCATCTTC ATCTGCATTG TCAGACATAC AACAATTCAC AAACACTTAT
    CAACAGCTGG AAACAAGGCG AAGTGAGCAG TCGGCTGCTA ACCTGCTGAG GCAGAAGTTG
    CAGGAGACTG GCATGGGCTT TGACGATTAC CCAGGAGGGG ATTACTATGG GACCACTGCT
    GGCCATAGTC CTGGACATCA CCTACTGTCC CGAGGTTCAC AAAACCAGAT GACTTCTCTC
    AGGTTGACAA GTCCAGACTC TAAAGCATCA GAAAGCACTG TGCCCAAAGG TTATTTCCCA
    TCAGGAAAGA AAAAAGGCCG CCCAGTCGGT AGTGTGAATA AGCAGAAACG TGCCCAAGGA
    CAAATGCAGA ATACAGCAAC TAACATACCT GCAGCTCCTC TTCCCTCACC TACAGCTGCA
    CCTCAGTCTG TTTCAGTCTC AAGCAATGCT GATGAAGTGC CCCGAGTACC AACACCACCT
    GATGAGAAAC CCTGCCCATC TCTGGTCCCT CCCGATGAAA CCCAAGTGGT GAAGGTGGAT
    GCAGAAAGCG AGGAGACCCT GCCAGAGTTG GATGTAAAGC CTGTTAAACA AAAACAGAAA
    AAGGGGAAAG AGTGCAGCGA GACTCCTGCC TCAAGTGGCA ATCAGAGAAG GAGAAGAAGA
    GGAATGGCTT CCAAAGAGGA GCTGGACACT CAGACGAGCA GTAGGGGAAG CATTAGTCTC
    AGTGGAATAT TCTCTGATGC CAGGAATAGT GTTTTTGCTC CTTACGTACA TGTGGAGAAA
    AAGATGGAAG AAATTGGGGC AGTCTGTACA ATAATTAACG CAGAGGATGA AAAGTCAAAA
    GGTGGAGGCA AACCTGGTCA CACGGGGGTT GATGGCCCTT TAAACACCCT GCTGCCATCT
    CAGCTTGTGA GAAAAGAAAA GGAGAATGAA AAATCAAAAG AGAACTGGGT TTCAGAGCAA
    GTTGATTCAA CCTTGGAGTC AGGGAAAGCA CTTCCTACAT CCGAGTATGT TCTGCCAGGG
    CCTGTGATAT CCGAAACTGG ACACAGCGGT CACCTGTTGT GCTGCCTTTG TCAGAAATGG
    GCCAACTACA AACATCTTGG AGATCTCTAT GGTCCTTTTT ACCCAGCCGA GTATGCAGCC
    AAGCTCCCTA AGAATCAACC TCAAGTCCGA CAAACTTTGT CCCATTTTGG GCCAGCTGCC
    ACAGGTATGA CATCCATTCC AACAGAAGCG ATTCCCCAGG ATACAAGATT ACAAGAACCT
    CAGAATGTCA AGTCATCTGC AGAAAGCGAT TGCACAGTAA GCCAGGCAAC AAACACAACA
    TCCCCAGCCA CTATCATTGG CACTGTTTCT CCAATGCCTT TGGAAATGCC TTTCCAGAAT
    GCTATAGCCA GCAGCTCTAC TGCAAGGGTA ACAACTCATG CTTGGGATAC GGCAGCCGAG
    CTGGAAAGTG GACTGGGTAC ATCAAATTTA CAAGAAACAG ACGCTGAAAT CGCATCAAAG
    CAGTTGCAGG TAGAAAGTTC ACAGCAAAGG CCCCAGCACA GAAAACTGAC ATCTCATCCA
    CGCTTCAAAA GAAGACACAA ATCCAGCGAA GATTTGCCTC GAACTGTCCC AATTAACAGC
    AAAGCCTCCC TGCCCTTCCA GCCTCCACCC CCAAGCCTGG ATGCTCTGGG CCCCATGGCT
    CAGCTGGCGC AGCTCCCGCT GGTGCCTCTG GACCCTGAGG AGCTGTGGGT GCACGAGAGC
    TGCATGGTGT GGACCAGTGG CATATACCTG GTCAATGGGA GACTCTATGG CCTTCAGGAA
    GCACTGGATG GTGCTAGAGA TGCGAGCTGC TCTCATTGTG AGATGGCGGG CTCCACCCTC
    GGCTGCTACA GTAAAGGCTG CACGCTGAGG TACCACTACA TTTGTGCCAT AGACGCAGAT
    TGCTCTCTTA ACGAAGACAA CTTTTCTTTG AGGTGTCCAA AGCACAAGGG CAACAGGATG
    GCCAAGCCTG GAGTGGTGTA TCTGGAGCAG TCGGAGCGCG GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009297282.1
    CDS1055..8119
    Translation

    Target ORF information:

    RefSeq Version XM_009299007.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio transcription factor 20 (tcf20), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299007.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCAGAATT TCCCCAACAG TCCTGTCCCT CTCCACCCAG GGTTCAGCCG TGGAGCAGGC 
    GTAGTCAGTT CCCCATATCA GACCCACCCG TCAGACCTTC AGATCTCCCC CAGGACCTCC
    GAGGACTATG TGTCCCTTCA GCAGGCCCAG ACCAGCCTAC AGAGCCACGG GCAGCACTTG
    CACCTGCGGG CCCAGCAGCA GCAGCACCTG CATGCTCCGG TACATGGCTA CAGCTCCAGG
    CGAGCGGTCA CAGAGATGCC GCAGAGCAGC GCGGGGGGGA ACTCCTACCG AAAGGACAGT
    ATGGATTATT ACTTCTCTGT GAGTGGGCGA GACCGAAGCC GAAGAGGTGG AGCCGCTTAC
    GGAGCCGGAT TTAGGTACAC AAACGTGGAT GGGCACCAGT ACCAACACCT CTCCGGTTCA
    GGGTCTTCCT CCGGAATGAT TTCTCCCTAC TCCATGGACT GTGGCTCTGC AGTCGTGGGG
    AGTAATAGCA GTGCAGCTGG TTCGTTTTCT CCTTCCCAGC AATACAGTAT GGCTCACGCT
    GCTTCAGTAC AATCAAGTGC TCAAATGCAT CAACGGCAGA AATATCCCTC ACATCAAGGC
    TTATTACACC AAGGTCAGCA TCACCGGACA TATGCGCTCT CCGGAAACAG AATCCCCGCT
    CAGTTCGGAC ACTACGCCAC TATCAATGCA ACCTCAGGCT CCTCTGGAAT GTATAACTCT
    CCACCGCAGA GATACGACGT GAGCAGCAGT AGCAGCACAG AAGCCAAAAT CAATGACTCT
    CCCACGCAGT CTAATCCAAA TCATCATAGC AGTTCATCAG CTTCCAACAG CTGCGAGGCT
    ATGGGGCAGG GCTACTCCTC GTCTGCACAT CCACCGTATT CACCACAGTC GCTTCACAAG
    CACGGCCCCC ATTCCCAGCG TACCCCTCAA CACAGCGCTG TCGCAGGCTA TGACCTATCC
    CTCAAAATGC AGCATCTGCA CACCAAACTG CCCCATTCCT CTGTCTCCTC CAGCCCGTCT
    CTGCCCTCAC ACACCCCTCA AGACCTCACC AAGTCTCCTA TGCACACTCA AAATCAACAG
    GCTCAGATTC ATCAGAACTT CAGTCCTATC TCTAATCCCT CCCCAGCTCC ATCTGCGGTG
    CAGTCTCCAA GCTGCAGCTC GTCCTCATCT CCACTGCTAG GGGTCTCGGA GGGAAACAGT
    GCTCAGCTGC AGGCGTCTTC ACACCCTGCT GCCCCAAACG CACGCAGCAG CCAGAGTCAC
    GCACGCCTCC TGCAGGCCGT CCCCCAGCTG AGCCCCACTC CCCATTCCAA TAGCAGCATC
    AGTAGCTGTG GTAGCGGAGG TGGAAATAAG TCTGCCACTC TGAATCACAG TGCAGGGATT
    GGTCATGCCA ATATGGGGCA GAACCGAATG GGGCCAGGTC TCCGGAGTGG TCCGGGGGAA
    GATAGCTCCC TGTTCCCTCA TGACAAACAC CTACCGGACC CTGGTCTAAA CAGTCTCAAT
    GCTCTGACTT CTCAAGTGGA AAACCTACCG AATACAGTCC AGCACATGCT GCTAACCGAC
    ACTGTGCTGC CCCAGAGGAA GAGCAGAGAC GCTGCCCCTC ACTTTCAGAT GCAGCAGGGG
    TCTCACTCTT TACCTGGCAA CCAGAACAAA TCTGGGAACT CCAGCACTGC TGGTCGGATC
    TTAACAAATG AAGAGAGCTC TGAGATGCTG GAAACAAAAG GCCAACAAAA ATTAGAAAGG
    AAAAGAGTTA GGCAGGAAAG TGGTACAAGC AATGAATCTG AGCCACAGAG CTACCTGCCA
    TCACAAAGGC AGATGCCCTC AGATAAGAGT CAGCAGGGCC TGGATCTCCA GGTCAACACT
    GGAGTCATCT CAACAGCTTC AAAACAGTCT TCTGTTAGAG AGTCTCAAGC AAAGACTCCA
    GAAACCCTCA CCCCCTCCTC CACTTCACCA CCATCAGGTC ATCCTACTGC AGAACCCAGC
    CCAAAGCTAC AGGTGTATCC CCCTGCTCCA GCCTCTCCCA TTCACTCCGC ATGTCTAAAC
    TCTGTGGCAG ATAGAGACCT AGGTAAAGAT GATGAGAAGG GAACTAAGGG ACCACAAATC
    AAAATGATAA AAGATGAAGA AAGCGATGAT GAATGTGCAG ATCCTCCGTG TCATAGAACG
    AGTTCAGAGA ACAGACTGTT GGTTCCTCTC AAACAGGCCG AGCGAGAGTC CTGTGCTCAA
    TCATTAGAAA ATCCCAGTAA AAATGTGAAT GTTCCAGAAC AACACAATGT TGGAGGTGTA
    GGGGTTATTG TCTCTGCTCG TTCTGAACTG AACACAGAAT CAAGCAAGCA CACAGGAGTC
    AAATCTCCAC GTTACAGTGT TTCTCATTAC CCAAACAAAC CCAGCAGTAT TGAGGAGCAG
    CATGATGTCA ATGTCTTAAA AGAGACGAGA AACCAGAATG GAGAAGGAGA GATGTCCATG
    GAAGCATATG TGTCACAGTA TGATATTTCC CCTAAACGAG AATTTGGCCA AAACATTGTT
    TCTGTTCAAT CTCACCCAGT GTCATTTAAA TGCAGCAATC CAGAGGTACA ATACACTGCC
    GTAAAGAGCA AGGACAAATT AGGGCTAGGT AGTGTTATGG GTGCAAACAG AGGCCAGAAT
    TACCATACAC TACAGGCCAG CTATGGGTCT ATTGCCAGAA AGGAGATTGC TGGTGTATTT
    GCTATGGAGG GGGGAAGAGG AATGGTCTCA AGAAGCCAGG ATAACAATTC TCAGTTTCAG
    CAGGTATTTC CAAGCCTTTT ACAAGAAGTC CTTCAAGGCC ATCACTTGGA TCGCCGCTAT
    GTACGTGCTG AACAGACATC TAGTGCTCAT CAACATTCTC AAGATACGTC TCAGCACCGT
    TTTCAAACCA GGCTACCTTA CAGTATGATT GAAAACTTGA GTTCACATTC AATGGGCTCT
    CAATCAGTTA TGGGGGGACT TAATGTCCAG TTTAGTCAAT TGGCCCCTGG AAAATCTCCA
    AACTCAAGTC AGGGACCAGA TAGTGAGATT GGTTTAGGCC CTTCTCATCC CTCTTGGGAT
    TCTGAAGCAC ATAAGACTCA CGGGATCTCC ATAGAGAAAA GCAAAACTAG TCTGTCTCCC
    AGCCAGTCCT CCCACATGCA GCAGTCTTTA GATCTCTCAA CAGGAGCCCC TCCAAAGCAC
    ATCAACCTGG CTGACTATTC TTTGCAGCAC AGAAAACCAT CCAGATATGG TACCTCTCCT
    TCAGCTGTAG AACAATTGCT TTTACAGGAA GCTGAGCCAC TGGCATGCAA TGTAGGTCCT
    GTCGGCCATG TTCAATCCCA GGCATCCTCA TCCTCTGGAA GGCGCTCGGT AATCTGTGAT
    GTGTCGCCTT CTCGACGAAC AACACCAGAA AGAGAGAGAG GATCCTCTGG ACCCTCAGTC
    ATTCAGCAGC CGTTTTCTTC TGGGTCTAAT GAGCAAGACT GCTGCAAGGA AGAAATTAAA
    GCAAAGAAAG CACAAATCAA GGAGGACCCA TCAAAGGTTG AAGCTGCAGG TCAGAACACA
    GATCGCTACT GCAGCACACC ACCCAGTAAG GAGTCTAATA AGCCTCTTCG CCATCCTGTA
    GATAGTGATC CTGACTTAGA AAAGACCAGC AGTGCCAAGG GCAACAGTGA TGCCCTCAGC
    AACCTGCCAT ATCTTTCACA AATGCCCACA AACCCCTTGT CCTCACCACA GAGACTCCAG
    TCATTTTCAC AGGCTGTTGA TGGCTTAAGG GCATATGGCT TCGGTGATGC AATGGATGGA
    CAAAAAATGA TCCCTCATGC TGGACCACAT CAACCGTTCC ATACGCTATC AGCATATACT
    AAGACCACAG CCTCTACAAA CAAGTTACAA GTGTTTCCAC AGAGTTTGCC TCCTCAAGAC
    AGACCTGACT GGACTCCTGA CAGACAAAAG CTTCGAGGTA TGGACAGGCA CGGTCCTCAG
    AGAATCCCCG AACAGAAATG TAAAAGCCAG CCTTCAAGTG AAATTCTGTC TAGTCCACAT
    CGTATATCTC AACAGCACTC TTATCCTGGT GCACAGTTTG ACCTGAAAAT GTGGGATGCT
    TATCCTGAAA AAGAGGGAGC TGCGCAACCA CTGGCTGCAG TCCCTCGTGA GCATGTTGGT
    TCTCAACCAA TTACAAATTC TGGCCCCAAA ATTGCAAAAG AGGACATCTC AGATAAGGGT
    GCAGAAGACA CAGTCAAATC CTTCCACTCA TTGGCACCGG CTAGTACTAA TGCTGGGCAC
    ACCGTCAAGG CAACCCAAGG CATTCAGCAG GGGCAGAGAG TAACTAAAAC TGGAGCATTG
    GCTGAAACTA ATCCCCTAAT CATGAGAAGG AGAGTTAGAT CTTTTATTTC CCCTATTCCT
    GCTAAGAGGC AGCATCAGGA TGTTCCAGGT CAGCGGCCAG GATCAGCATA CCACTCCCCA
    ATAGCTCAAG CCTACCCTGA ATCTAGAATT GCAACCAACA GTTGTTCTGG TAGTTCGGAT
    TCTCAGGCCA AATTACCATC TCCCAAGACA CCGCATTCCA TATCTAGTGC TAACTCGCCT
    CCTCAGTCCA AAGCAAAGTT TCTTCCCCCA AGAAAGGGGC GTGGCTTGAA ACTTGAGGCG
    ATTGTGCAGA AAATTACTCC TGGCGTGAGA AAAAATGACT TCAACAATAG TAACTTGGAA
    TCGGACTTTT CAGAAGCAGC TCATTACACC TCTGACTTGC CGGACCCAGA GAGTGGTACA
    TCGTTTACTA GTGTTTCTCA AGGTGAGGAG ACCTGTTTAT CTTACCTTGA GGATTCTCAT
    ACTTTAGATG AGCTTATGCC ATACAGAGCA GTTGAAGATG CATTCTCATG TGACTCTCAG
    TCTCTCAAAG CAGGTGCAGC TGCTTCTAGC AGCGGTGCCC TCAGAAGTTT GCCAAAAGAT
    TTCGACTTTG GCTTAGGTAC TGCTGGCTCT TCAGGGTCTT TGGGGGGTGA AAATGATAAG
    GATGAATTTA CTTTGTTGGG ACCCCTTCCA CCAGCTCCTC CATTGCCTTG TCCTGTACAG
    GGATCACCTC CTCCATCTTC ATCTGCATTG TCAGACATAC AACAATTCAC AAACACTTAT
    CAACAGCTGG AAACAAGGCG AAGTGAGCAG TCGGCTGCTA ACCTGCTGAG GCAGAAGTTG
    CAGGAGACTG GCATGGGCTT TGACGATTAC CCAGGAGGGG ATTACTATGG GACCACTGCT
    GGCCATAGTC CTGGACATCA CCTACTGTCC CGAGGTTCAC AAAACCAGAT GACTTCTCTC
    AGGTTGACAA GTCCAGACTC TAAAGCATCA GAAAGCACTG TGCCCAAAGG TTATTTCCCA
    TCAGGAAAGA AAAAAGGCCG CCCAGTCGGT AGTGTGAATA AGCAGAAACG TGCCCAAGGA
    CAAATGCAGA ATACAGCAAC TAACATACCT GCAGCTCCTC TTCCCTCACC TACAGCTGCA
    CCTCAGTCTG TTTCAGTCTC AAGCAATGCT GATGAAGTGC CCCGAGTACC AACACCACCT
    GATGAGAAAC CCTGCCCATC TCTGGTCCCT CCCGATGAAA CCCAAGTGGT GAAGGTGGAT
    GCAGAAAGCG AGGAGACCCT GCCAGAGTTG GATGTAAAGC CTGTTAAACA AAAACAGAAA
    AAGGGGAAAG AGTGCAGCGA GACTCCTGCC TCAAGTGGCA ATCAGAGAAG GAGAAGAAGA
    GGAATGGCTT CCAAAGAGGA GCTGGACACT CAGACGAGCA GTAGGGGAAG CATTAGTCTC
    AGTGGAATAT TCTCTGATGC CAGGAATAGT GTTTTTGCTC CTTACGTACA TGTGGAGAAA
    AAGATGGAAG AAATTGGGGC AGTCTGTACA ATAATTAACG CAGAGGATGA AAAGTCAAAA
    GGTGGAGGCA AACCTGGTCA CACGGGGGTT GATGGCCCTT TAAACACCCT GCTGCCATCT
    CAGCTTGTGA GAAAAGAAAA GGAGAATGAA AAATCAAAAG AGAACTGGGT TTCAGAGCAA
    GTTGATTCAA CCTTGGAGTC AGGGAAAGCA CTTCCTACAT CCGAGTATGT TCTGCCAGGG
    CCTGTGATAT CCGAAACTGG ACACAGCGGT CACCTGTTGT GCTGCCTTTG TCAGAAATGG
    GCCAACTACA AACATCTTGG AGATCTCTAT GGTCCTTTTT ACCCAGCCGA GTATGCAGCC
    AAGCTCCCTA AGAATCAACC TCAAGTCCGA CAAACTTTGT CCCATTTTGG GCCAGCTGCC
    ACAGGTATGA CATCCATTCC AACAGAAGCG ATTCCCCAGG ATACAAGATT ACAAGAACCT
    CAGAATGTCA AGTCATCTGC AGAAAGCGAT TGCACAGTAA GCCAGGCAAC AAACACAACA
    TCCCCAGCCA CTATCATTGG CACTGTTTCT CCAATGCCTT TGGAAATGCC TTTCCAGAAT
    GCTATAGCCA GCAGCTCTAC TGCAAGGGTA ACAACTCATG CTTGGGATAC GGCAGCCGAG
    CTGGAAAGTG GACTGGGTAC ATCAAATTTA CAAGAAACAG ACGCTGAAAT CGCATCAAAG
    CAGTTGCAGG TAGAAAGTTC ACAGCAAAGG CCCCAGCACA GAAAACTGAC ATCTCATCCA
    CGCTTCAAAA GAAGACACAA ATCCAGCGAA GATTTGCCTC GAACTGTCCC AATTAACAGC
    AAAGCCTCCC TGCCCTTCCA GCCTCCACCC CCAAGCCTGG ATGCTCTGGG CCCCATGGCT
    CAGCTGGCGC AGCTCCCGCT GGTGCCTCTG GACCCTGAGG AGCTGTGGGT GCACGAGAGC
    TGCATGGTGT GGACCAGTGG CATATACCTG GTCAATGGGA GACTCTATGG CCTTCAGGAA
    GCACTGGATG GTGCTAGAGA TGCGAGCTGC TCTCATTGTG AGATGGCGGG CTCCACCCTC
    GGCTGCTACA GTAAAGGCTG CACGCTGAGG TACCACTACA TTTGTGCCAT AGACGCAGAT
    TGCTCTCTTA ACGAAGACAA CTTTTCTTTG AGGTGTCCAA AGCACAAGGG CAACAGGATG
    GCCAAGCCTG GAGTGGTGTA TCTGGAGCAG TCGGAGCGCG GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa07773
    Clone ID Related Accession (Same CDS sequence) XM_021472464.1 , XM_005171536.3 , XM_009299007.2 , XM_005171536.4
    Accession Version XM_005171536.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product transcription factor 20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171536.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCAGAATT TCCCCAACAG TCCTGTCCCT CTCCACCCAG GGTTCAGCCG TGGAGCAGGC 
    GTAGTCAGTT CCCCATATCA GACCCACCCG TCAGACCTTC AGATCTCCCC CAGGACCTCC
    GAGGACTATG TGTCCCTTCA GCAGGCCCAG ACCAGCCTAC AGAGCCACGG GCAGCACTTG
    CACCTGCGGG CCCAGCAGCA GCAGCACCTG CATGCTCCGG TACATGGCTA CAGCTCCAGG
    CGAGCGGTCA CAGAGATGCC GCAGAGCAGC GCGGGGGGGA ACTCCTACCG AAAGGACAGT
    ATGGATTATT ACTTCTCTGT GAGTGGGCGA GACCGAAGCC GAAGAGGTGG AGCCGCTTAC
    GGAGCCGGAT TTAGGTACAC AAACGTGGAT GGGCACCAGT ACCAACACCT CTCCGGTTCA
    GGGTCTTCCT CCGGAATGAT TTCTCCCTAC TCCATGGACT GTGGCTCTGC AGTCGTGGGG
    AGTAATAGCA GTGCAGCTGG TTCGTTTTCT CCTTCCCAGC AATACAGTAT GGCTCACGCT
    GCTTCAGTAC AATCAAGTGC TCAAATGCAT CAACGGCAGA AATATCCCTC ACATCAAGGC
    TTATTACACC AAGGTCAGCA TCACCGGACA TATGCGCTCT CCGGAAACAG AATCCCCGCT
    CAGTTCGGAC ACTACGCCAC TATCAATGCA ACCTCAGGCT CCTCTGGAAT GTATAACTCT
    CCACCGCAGA GATACGACGT GAGCAGCAGT AGCAGCACAG AAGCCAAAAT CAATGACTCT
    CCCACGCAGT CTAATCCAAA TCATCATAGC AGTTCATCAG CTTCCAACAG CTGCGAGGCT
    ATGGGGCAGG GCTACTCCTC GTCTGCACAT CCACCGTATT CACCACAGTC GCTTCACAAG
    CACGGCCCCC ATTCCCAGCG TACCCCTCAA CACAGCGCTG TCGCAGGCTA TGACCTATCC
    CTCAAAATGC AGCATCTGCA CACCAAACTG CCCCATTCCT CTGTCTCCTC CAGCCCGTCT
    CTGCCCTCAC ACACCCCTCA AGACCTCACC AAGTCTCCTA TGCACACTCA AAATCAACAG
    GCTCAGATTC ATCAGAACTT CAGTCCTATC TCTAATCCCT CCCCAGCTCC ATCTGCGGTG
    CAGTCTCCAA GCTGCAGCTC GTCCTCATCT CCACTGCTAG GGGTCTCGGA GGGAAACAGT
    GCTCAGCTGC AGGCGTCTTC ACACCCTGCT GCCCCAAACG CACGCAGCAG CCAGAGTCAC
    GCACGCCTCC TGCAGGCCGT CCCCCAGCTG AGCCCCACTC CCCATTCCAA TAGCAGCATC
    AGTAGCTGTG GTAGCGGAGG TGGAAATAAG TCTGCCACTC TGAATCACAG TGCAGGGATT
    GGTCATGCCA ATATGGGGCA GAACCGAATG GGGCCAGGTC TCCGGAGTGG TCCGGGGGAA
    GATAGCTCCC TGTTCCCTCA TGACAAACAC CTACCGGACC CTGGTCTAAA CAGTCTCAAT
    GCTCTGACTT CTCAAGTGGA AAACCTACCG AATACAGTCC AGCACATGCT GCTAACCGAC
    ACTGTGCTGC CCCAGAGGAA GAGCAGAGAC GCTGCCCCTC ACTTTCAGAT GCAGCAGGGG
    TCTCACTCTT TACCTGGCAA CCAGAACAAA TCTGGGAACT CCAGCACTGC TGGTCGGATC
    TTAACAAATG AAGAGAGCTC TGAGATGCTG GAAACAAAAG GCCAACAAAA ATTAGAAAGG
    AAAAGAGTTA GGCAGGAAAG TGGTACAAGC AATGAATCTG AGCCACAGAG CTACCTGCCA
    TCACAAAGGC AGATGCCCTC AGATAAGAGT CAGCAGGGCC TGGATCTCCA GGTCAACACT
    GGAGTCATCT CAACAGCTTC AAAACAGTCT TCTGTTAGAG AGTCTCAAGC AAAGACTCCA
    GAAACCCTCA CCCCCTCCTC CACTTCACCA CCATCAGGTC ATCCTACTGC AGAACCCAGC
    CCAAAGCTAC AGGTGTATCC CCCTGCTCCA GCCTCTCCCA TTCACTCCGC ATGTCTAAAC
    TCTGTGGCAG ATAGAGACCT AGGTAAAGAT GATGAGAAGG GAACTAAGGG ACCACAAATC
    AAAATGATAA AAGATGAAGA AAGCGATGAT GAATGTGCAG ATCCTCCGTG TCATAGAACG
    AGTTCAGAGA ACAGACTGTT GGTTCCTCTC AAACAGGCCG AGCGAGAGTC CTGTGCTCAA
    TCATTAGAAA ATCCCAGTAA AAATGTGAAT GTTCCAGAAC AACACAATGT TGGAGGTGTA
    GGGGTTATTG TCTCTGCTCG TTCTGAACTG AACACAGAAT CAAGCAAGCA CACAGGAGTC
    AAATCTCCAC GTTACAGTGT TTCTCATTAC CCAAACAAAC CCAGCAGTAT TGAGGAGCAG
    CATGATGTCA ATGTCTTAAA AGAGACGAGA AACCAGAATG GAGAAGGAGA GATGTCCATG
    GAAGCATATG TGTCACAGTA TGATATTTCC CCTAAACGAG AATTTGGCCA AAACATTGTT
    TCTGTTCAAT CTCACCCAGT GTCATTTAAA TGCAGCAATC CAGAGGTACA ATACACTGCC
    GTAAAGAGCA AGGACAAATT AGGGCTAGGT AGTGTTATGG GTGCAAACAG AGGCCAGAAT
    TACCATACAC TACAGGCCAG CTATGGGTCT ATTGCCAGAA AGGAGATTGC TGGTGTATTT
    GCTATGGAGG GGGGAAGAGG AATGGTCTCA AGAAGCCAGG ATAACAATTC TCAGTTTCAG
    CAGGTATTTC CAAGCCTTTT ACAAGAAGTC CTTCAAGGCC ATCACTTGGA TCGCCGCTAT
    GTACGTGCTG AACAGACATC TAGTGCTCAT CAACATTCTC AAGATACGTC TCAGCACCGT
    TTTCAAACCA GGCTACCTTA CAGTATGATT GAAAACTTGA GTTCACATTC AATGGGCTCT
    CAATCAGTTA TGGGGGGACT TAATGTCCAG TTTAGTCAAT TGGCCCCTGG AAAATCTCCA
    AACTCAAGTC AGGGACCAGA TAGTGAGATT GGTTTAGGCC CTTCTCATCC CTCTTGGGAT
    TCTGAAGCAC ATAAGACTCA CGGGATCTCC ATAGAGAAAA GCAAAACTAG TCTGTCTCCC
    AGCCAGTCCT CCCACATGCA GCAGTCTTTA GATCTCTCAA CAGGAGCCCC TCCAAAGCAC
    ATCAACCTGG CTGACTATTC TTTGCAGCAC AGAAAACCAT CCAGATATGG TACCTCTCCT
    TCAGCTGTAG AACAATTGCT TTTACAGGAA GCTGAGCCAC TGGCATGCAA TGTAGGTCCT
    GTCGGCCATG TTCAATCCCA GGCATCCTCA TCCTCTGGAA GGCGCTCGGT AATCTGTGAT
    GTGTCGCCTT CTCGACGAAC AACACCAGAA AGAGAGAGAG GATCCTCTGG ACCCTCAGTC
    ATTCAGCAGC CGTTTTCTTC TGGGTCTAAT GAGCAAGACT GCTGCAAGGA AGAAATTAAA
    GCAAAGAAAG CACAAATCAA GGAGGACCCA TCAAAGGTTG AAGCTGCAGG TCAGAACACA
    GATCGCTACT GCAGCACACC ACCCAGTAAG GAGTCTAATA AGCCTCTTCG CCATCCTGTA
    GATAGTGATC CTGACTTAGA AAAGACCAGC AGTGCCAAGG GCAACAGTGA TGCCCTCAGC
    AACCTGCCAT ATCTTTCACA AATGCCCACA AACCCCTTGT CCTCACCACA GAGACTCCAG
    TCATTTTCAC AGGCTGTTGA TGGCTTAAGG GCATATGGCT TCGGTGATGC AATGGATGGA
    CAAAAAATGA TCCCTCATGC TGGACCACAT CAACCGTTCC ATACGCTATC AGCATATACT
    AAGACCACAG CCTCTACAAA CAAGTTACAA GTGTTTCCAC AGAGTTTGCC TCCTCAAGAC
    AGACCTGACT GGACTCCTGA CAGACAAAAG CTTCGAGGTA TGGACAGGCA CGGTCCTCAG
    AGAATCCCCG AACAGAAATG TAAAAGCCAG CCTTCAAGTG AAATTCTGTC TAGTCCACAT
    CGTATATCTC AACAGCACTC TTATCCTGGT GCACAGTTTG ACCTGAAAAT GTGGGATGCT
    TATCCTGAAA AAGAGGGAGC TGCGCAACCA CTGGCTGCAG TCCCTCGTGA GCATGTTGGT
    TCTCAACCAA TTACAAATTC TGGCCCCAAA ATTGCAAAAG AGGACATCTC AGATAAGGGT
    GCAGAAGACA CAGTCAAATC CTTCCACTCA TTGGCACCGG CTAGTACTAA TGCTGGGCAC
    ACCGTCAAGG CAACCCAAGG CATTCAGCAG GGGCAGAGAG TAACTAAAAC TGGAGCATTG
    GCTGAAACTA ATCCCCTAAT CATGAGAAGG AGAGTTAGAT CTTTTATTTC CCCTATTCCT
    GCTAAGAGGC AGCATCAGGA TGTTCCAGGT CAGCGGCCAG GATCAGCATA CCACTCCCCA
    ATAGCTCAAG CCTACCCTGA ATCTAGAATT GCAACCAACA GTTGTTCTGG TAGTTCGGAT
    TCTCAGGCCA AATTACCATC TCCCAAGACA CCGCATTCCA TATCTAGTGC TAACTCGCCT
    CCTCAGTCCA AAGCAAAGTT TCTTCCCCCA AGAAAGGGGC GTGGCTTGAA ACTTGAGGCG
    ATTGTGCAGA AAATTACTCC TGGCGTGAGA AAAAATGACT TCAACAATAG TAACTTGGAA
    TCGGACTTTT CAGAAGCAGC TCATTACACC TCTGACTTGC CGGACCCAGA GAGTGGTACA
    TCGTTTACTA GTGTTTCTCA AGGTGAGGAG ACCTGTTTAT CTTACCTTGA GGATTCTCAT
    ACTTTAGATG AGCTTATGCC ATACAGAGCA GTTGAAGATG CATTCTCATG TGACTCTCAG
    TCTCTCAAAG CAGGTGCAGC TGCTTCTAGC AGCGGTGCCC TCAGAAGTTT GCCAAAAGAT
    TTCGACTTTG GCTTAGGTAC TGCTGGCTCT TCAGGGTCTT TGGGGGGTGA AAATGATAAG
    GATGAATTTA CTTTGTTGGG ACCCCTTCCA CCAGCTCCTC CATTGCCTTG TCCTGTACAG
    GGATCACCTC CTCCATCTTC ATCTGCATTG TCAGACATAC AACAATTCAC AAACACTTAT
    CAACAGCTGG AAACAAGGCG AAGTGAGCAG TCGGCTGCTA ACCTGCTGAG GCAGAAGTTG
    CAGGAGACTG GCATGGGCTT TGACGATTAC CCAGGAGGGG ATTACTATGG GACCACTGCT
    GGCCATAGTC CTGGACATCA CCTACTGTCC CGAGGTTCAC AAAACCAGAT GACTTCTCTC
    AGGTTGACAA GTCCAGACTC TAAAGCATCA GAAAGCACTG TGCCCAAAGG TTATTTCCCA
    TCAGGAAAGA AAAAAGGCCG CCCAGTCGGT AGTGTGAATA AGCAGAAACG TGCCCAAGGA
    CAAATGCAGA ATACAGCAAC TAACATACCT GCAGCTCCTC TTCCCTCACC TACAGCTGCA
    CCTCAGTCTG TTTCAGTCTC AAGCAATGCT GATGAAGTGC CCCGAGTACC AACACCACCT
    GATGAGAAAC CCTGCCCATC TCTGGTCCCT CCCGATGAAA CCCAAGTGGT GAAGGTGGAT
    GCAGAAAGCG AGGAGACCCT GCCAGAGTTG GATGTAAAGC CTGTTAAACA AAAACAGAAA
    AAGGGGAAAG AGTGCAGCGA GACTCCTGCC TCAAGTGGCA ATCAGAGAAG GAGAAGAAGA
    GGAATGGCTT CCAAAGAGGA GCTGGACACT CAGACGAGCA GTAGGGGAAG CATTAGTCTC
    AGTGGAATAT TCTCTGATGC CAGGAATAGT GTTTTTGCTC CTTACGTACA TGTGGAGAAA
    AAGATGGAAG AAATTGGGGC AGTCTGTACA ATAATTAACG CAGAGGATGA AAAGTCAAAA
    GGTGGAGGCA AACCTGGTCA CACGGGGGTT GATGGCCCTT TAAACACCCT GCTGCCATCT
    CAGCTTGTGA GAAAAGAAAA GGAGAATGAA AAATCAAAAG AGAACTGGGT TTCAGAGCAA
    GTTGATTCAA CCTTGGAGTC AGGGAAAGCA CTTCCTACAT CCGAGTATGT TCTGCCAGGG
    CCTGTGATAT CCGAAACTGG ACACAGCGGT CACCTGTTGT GCTGCCTTTG TCAGAAATGG
    GCCAACTACA AACATCTTGG AGATCTCTAT GGTCCTTTTT ACCCAGCCGA GTATGCAGCC
    AAGCTCCCTA AGAATCAACC TCAAGTCCGA CAAACTTTGT CCCATTTTGG GCCAGCTGCC
    ACAGGTATGA CATCCATTCC AACAGAAGCG ATTCCCCAGG ATACAAGATT ACAAGAACCT
    CAGAATGTCA AGTCATCTGC AGAAAGCGAT TGCACAGTAA GCCAGGCAAC AAACACAACA
    TCCCCAGCCA CTATCATTGG CACTGTTTCT CCAATGCCTT TGGAAATGCC TTTCCAGAAT
    GCTATAGCCA GCAGCTCTAC TGCAAGGGTA ACAACTCATG CTTGGGATAC GGCAGCCGAG
    CTGGAAAGTG GACTGGGTAC ATCAAATTTA CAAGAAACAG ACGCTGAAAT CGCATCAAAG
    CAGTTGCAGG TAGAAAGTTC ACAGCAAAGG CCCCAGCACA GAAAACTGAC ATCTCATCCA
    CGCTTCAAAA GAAGACACAA ATCCAGCGAA GATTTGCCTC GAACTGTCCC AATTAACAGC
    AAAGCCTCCC TGCCCTTCCA GCCTCCACCC CCAAGCCTGG ATGCTCTGGG CCCCATGGCT
    CAGCTGGCGC AGCTCCCGCT GGTGCCTCTG GACCCTGAGG AGCTGTGGGT GCACGAGAGC
    TGCATGGTGT GGACCAGTGG CATATACCTG GTCAATGGGA GACTCTATGG CCTTCAGGAA
    GCACTGGATG GTGCTAGAGA TGCGAGCTGC TCTCATTGTG AGATGGCGGG CTCCACCCTC
    GGCTGCTACA GTAAAGGCTG CACGCTGAGG TACCACTACA TTTGTGCCAT AGACGCAGAT
    TGCTCTCTTA ACGAAGACAA CTTTTCTTTG AGGTGTCCAA AGCACAAGGG CAACAGGATG
    GCCAAGCCTG GAGTGGTGTA TCTGGAGCAG TCGGAGCGCG GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005171593.1
    CDS1051..8115
    Translation

    Target ORF information:

    RefSeq Version XM_005171536.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio transcription factor 20 (tcf20), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005171536.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCAGAATT TCCCCAACAG TCCTGTCCCT CTCCACCCAG GGTTCAGCCG TGGAGCAGGC 
    GTAGTCAGTT CCCCATATCA GACCCACCCG TCAGACCTTC AGATCTCCCC CAGGACCTCC
    GAGGACTATG TGTCCCTTCA GCAGGCCCAG ACCAGCCTAC AGAGCCACGG GCAGCACTTG
    CACCTGCGGG CCCAGCAGCA GCAGCACCTG CATGCTCCGG TACATGGCTA CAGCTCCAGG
    CGAGCGGTCA CAGAGATGCC GCAGAGCAGC GCGGGGGGGA ACTCCTACCG AAAGGACAGT
    ATGGATTATT ACTTCTCTGT GAGTGGGCGA GACCGAAGCC GAAGAGGTGG AGCCGCTTAC
    GGAGCCGGAT TTAGGTACAC AAACGTGGAT GGGCACCAGT ACCAACACCT CTCCGGTTCA
    GGGTCTTCCT CCGGAATGAT TTCTCCCTAC TCCATGGACT GTGGCTCTGC AGTCGTGGGG
    AGTAATAGCA GTGCAGCTGG TTCGTTTTCT CCTTCCCAGC AATACAGTAT GGCTCACGCT
    GCTTCAGTAC AATCAAGTGC TCAAATGCAT CAACGGCAGA AATATCCCTC ACATCAAGGC
    TTATTACACC AAGGTCAGCA TCACCGGACA TATGCGCTCT CCGGAAACAG AATCCCCGCT
    CAGTTCGGAC ACTACGCCAC TATCAATGCA ACCTCAGGCT CCTCTGGAAT GTATAACTCT
    CCACCGCAGA GATACGACGT GAGCAGCAGT AGCAGCACAG AAGCCAAAAT CAATGACTCT
    CCCACGCAGT CTAATCCAAA TCATCATAGC AGTTCATCAG CTTCCAACAG CTGCGAGGCT
    ATGGGGCAGG GCTACTCCTC GTCTGCACAT CCACCGTATT CACCACAGTC GCTTCACAAG
    CACGGCCCCC ATTCCCAGCG TACCCCTCAA CACAGCGCTG TCGCAGGCTA TGACCTATCC
    CTCAAAATGC AGCATCTGCA CACCAAACTG CCCCATTCCT CTGTCTCCTC CAGCCCGTCT
    CTGCCCTCAC ACACCCCTCA AGACCTCACC AAGTCTCCTA TGCACACTCA AAATCAACAG
    GCTCAGATTC ATCAGAACTT CAGTCCTATC TCTAATCCCT CCCCAGCTCC ATCTGCGGTG
    CAGTCTCCAA GCTGCAGCTC GTCCTCATCT CCACTGCTAG GGGTCTCGGA GGGAAACAGT
    GCTCAGCTGC AGGCGTCTTC ACACCCTGCT GCCCCAAACG CACGCAGCAG CCAGAGTCAC
    GCACGCCTCC TGCAGGCCGT CCCCCAGCTG AGCCCCACTC CCCATTCCAA TAGCAGCATC
    AGTAGCTGTG GTAGCGGAGG TGGAAATAAG TCTGCCACTC TGAATCACAG TGCAGGGATT
    GGTCATGCCA ATATGGGGCA GAACCGAATG GGGCCAGGTC TCCGGAGTGG TCCGGGGGAA
    GATAGCTCCC TGTTCCCTCA TGACAAACAC CTACCGGACC CTGGTCTAAA CAGTCTCAAT
    GCTCTGACTT CTCAAGTGGA AAACCTACCG AATACAGTCC AGCACATGCT GCTAACCGAC
    ACTGTGCTGC CCCAGAGGAA GAGCAGAGAC GCTGCCCCTC ACTTTCAGAT GCAGCAGGGG
    TCTCACTCTT TACCTGGCAA CCAGAACAAA TCTGGGAACT CCAGCACTGC TGGTCGGATC
    TTAACAAATG AAGAGAGCTC TGAGATGCTG GAAACAAAAG GCCAACAAAA ATTAGAAAGG
    AAAAGAGTTA GGCAGGAAAG TGGTACAAGC AATGAATCTG AGCCACAGAG CTACCTGCCA
    TCACAAAGGC AGATGCCCTC AGATAAGAGT CAGCAGGGCC TGGATCTCCA GGTCAACACT
    GGAGTCATCT CAACAGCTTC AAAACAGTCT TCTGTTAGAG AGTCTCAAGC AAAGACTCCA
    GAAACCCTCA CCCCCTCCTC CACTTCACCA CCATCAGGTC ATCCTACTGC AGAACCCAGC
    CCAAAGCTAC AGGTGTATCC CCCTGCTCCA GCCTCTCCCA TTCACTCCGC ATGTCTAAAC
    TCTGTGGCAG ATAGAGACCT AGGTAAAGAT GATGAGAAGG GAACTAAGGG ACCACAAATC
    AAAATGATAA AAGATGAAGA AAGCGATGAT GAATGTGCAG ATCCTCCGTG TCATAGAACG
    AGTTCAGAGA ACAGACTGTT GGTTCCTCTC AAACAGGCCG AGCGAGAGTC CTGTGCTCAA
    TCATTAGAAA ATCCCAGTAA AAATGTGAAT GTTCCAGAAC AACACAATGT TGGAGGTGTA
    GGGGTTATTG TCTCTGCTCG TTCTGAACTG AACACAGAAT CAAGCAAGCA CACAGGAGTC
    AAATCTCCAC GTTACAGTGT TTCTCATTAC CCAAACAAAC CCAGCAGTAT TGAGGAGCAG
    CATGATGTCA ATGTCTTAAA AGAGACGAGA AACCAGAATG GAGAAGGAGA GATGTCCATG
    GAAGCATATG TGTCACAGTA TGATATTTCC CCTAAACGAG AATTTGGCCA AAACATTGTT
    TCTGTTCAAT CTCACCCAGT GTCATTTAAA TGCAGCAATC CAGAGGTACA ATACACTGCC
    GTAAAGAGCA AGGACAAATT AGGGCTAGGT AGTGTTATGG GTGCAAACAG AGGCCAGAAT
    TACCATACAC TACAGGCCAG CTATGGGTCT ATTGCCAGAA AGGAGATTGC TGGTGTATTT
    GCTATGGAGG GGGGAAGAGG AATGGTCTCA AGAAGCCAGG ATAACAATTC TCAGTTTCAG
    CAGGTATTTC CAAGCCTTTT ACAAGAAGTC CTTCAAGGCC ATCACTTGGA TCGCCGCTAT
    GTACGTGCTG AACAGACATC TAGTGCTCAT CAACATTCTC AAGATACGTC TCAGCACCGT
    TTTCAAACCA GGCTACCTTA CAGTATGATT GAAAACTTGA GTTCACATTC AATGGGCTCT
    CAATCAGTTA TGGGGGGACT TAATGTCCAG TTTAGTCAAT TGGCCCCTGG AAAATCTCCA
    AACTCAAGTC AGGGACCAGA TAGTGAGATT GGTTTAGGCC CTTCTCATCC CTCTTGGGAT
    TCTGAAGCAC ATAAGACTCA CGGGATCTCC ATAGAGAAAA GCAAAACTAG TCTGTCTCCC
    AGCCAGTCCT CCCACATGCA GCAGTCTTTA GATCTCTCAA CAGGAGCCCC TCCAAAGCAC
    ATCAACCTGG CTGACTATTC TTTGCAGCAC AGAAAACCAT CCAGATATGG TACCTCTCCT
    TCAGCTGTAG AACAATTGCT TTTACAGGAA GCTGAGCCAC TGGCATGCAA TGTAGGTCCT
    GTCGGCCATG TTCAATCCCA GGCATCCTCA TCCTCTGGAA GGCGCTCGGT AATCTGTGAT
    GTGTCGCCTT CTCGACGAAC AACACCAGAA AGAGAGAGAG GATCCTCTGG ACCCTCAGTC
    ATTCAGCAGC CGTTTTCTTC TGGGTCTAAT GAGCAAGACT GCTGCAAGGA AGAAATTAAA
    GCAAAGAAAG CACAAATCAA GGAGGACCCA TCAAAGGTTG AAGCTGCAGG TCAGAACACA
    GATCGCTACT GCAGCACACC ACCCAGTAAG GAGTCTAATA AGCCTCTTCG CCATCCTGTA
    GATAGTGATC CTGACTTAGA AAAGACCAGC AGTGCCAAGG GCAACAGTGA TGCCCTCAGC
    AACCTGCCAT ATCTTTCACA AATGCCCACA AACCCCTTGT CCTCACCACA GAGACTCCAG
    TCATTTTCAC AGGCTGTTGA TGGCTTAAGG GCATATGGCT TCGGTGATGC AATGGATGGA
    CAAAAAATGA TCCCTCATGC TGGACCACAT CAACCGTTCC ATACGCTATC AGCATATACT
    AAGACCACAG CCTCTACAAA CAAGTTACAA GTGTTTCCAC AGAGTTTGCC TCCTCAAGAC
    AGACCTGACT GGACTCCTGA CAGACAAAAG CTTCGAGGTA TGGACAGGCA CGGTCCTCAG
    AGAATCCCCG AACAGAAATG TAAAAGCCAG CCTTCAAGTG AAATTCTGTC TAGTCCACAT
    CGTATATCTC AACAGCACTC TTATCCTGGT GCACAGTTTG ACCTGAAAAT GTGGGATGCT
    TATCCTGAAA AAGAGGGAGC TGCGCAACCA CTGGCTGCAG TCCCTCGTGA GCATGTTGGT
    TCTCAACCAA TTACAAATTC TGGCCCCAAA ATTGCAAAAG AGGACATCTC AGATAAGGGT
    GCAGAAGACA CAGTCAAATC CTTCCACTCA TTGGCACCGG CTAGTACTAA TGCTGGGCAC
    ACCGTCAAGG CAACCCAAGG CATTCAGCAG GGGCAGAGAG TAACTAAAAC TGGAGCATTG
    GCTGAAACTA ATCCCCTAAT CATGAGAAGG AGAGTTAGAT CTTTTATTTC CCCTATTCCT
    GCTAAGAGGC AGCATCAGGA TGTTCCAGGT CAGCGGCCAG GATCAGCATA CCACTCCCCA
    ATAGCTCAAG CCTACCCTGA ATCTAGAATT GCAACCAACA GTTGTTCTGG TAGTTCGGAT
    TCTCAGGCCA AATTACCATC TCCCAAGACA CCGCATTCCA TATCTAGTGC TAACTCGCCT
    CCTCAGTCCA AAGCAAAGTT TCTTCCCCCA AGAAAGGGGC GTGGCTTGAA ACTTGAGGCG
    ATTGTGCAGA AAATTACTCC TGGCGTGAGA AAAAATGACT TCAACAATAG TAACTTGGAA
    TCGGACTTTT CAGAAGCAGC TCATTACACC TCTGACTTGC CGGACCCAGA GAGTGGTACA
    TCGTTTACTA GTGTTTCTCA AGGTGAGGAG ACCTGTTTAT CTTACCTTGA GGATTCTCAT
    ACTTTAGATG AGCTTATGCC ATACAGAGCA GTTGAAGATG CATTCTCATG TGACTCTCAG
    TCTCTCAAAG CAGGTGCAGC TGCTTCTAGC AGCGGTGCCC TCAGAAGTTT GCCAAAAGAT
    TTCGACTTTG GCTTAGGTAC TGCTGGCTCT TCAGGGTCTT TGGGGGGTGA AAATGATAAG
    GATGAATTTA CTTTGTTGGG ACCCCTTCCA CCAGCTCCTC CATTGCCTTG TCCTGTACAG
    GGATCACCTC CTCCATCTTC ATCTGCATTG TCAGACATAC AACAATTCAC AAACACTTAT
    CAACAGCTGG AAACAAGGCG AAGTGAGCAG TCGGCTGCTA ACCTGCTGAG GCAGAAGTTG
    CAGGAGACTG GCATGGGCTT TGACGATTAC CCAGGAGGGG ATTACTATGG GACCACTGCT
    GGCCATAGTC CTGGACATCA CCTACTGTCC CGAGGTTCAC AAAACCAGAT GACTTCTCTC
    AGGTTGACAA GTCCAGACTC TAAAGCATCA GAAAGCACTG TGCCCAAAGG TTATTTCCCA
    TCAGGAAAGA AAAAAGGCCG CCCAGTCGGT AGTGTGAATA AGCAGAAACG TGCCCAAGGA
    CAAATGCAGA ATACAGCAAC TAACATACCT GCAGCTCCTC TTCCCTCACC TACAGCTGCA
    CCTCAGTCTG TTTCAGTCTC AAGCAATGCT GATGAAGTGC CCCGAGTACC AACACCACCT
    GATGAGAAAC CCTGCCCATC TCTGGTCCCT CCCGATGAAA CCCAAGTGGT GAAGGTGGAT
    GCAGAAAGCG AGGAGACCCT GCCAGAGTTG GATGTAAAGC CTGTTAAACA AAAACAGAAA
    AAGGGGAAAG AGTGCAGCGA GACTCCTGCC TCAAGTGGCA ATCAGAGAAG GAGAAGAAGA
    GGAATGGCTT CCAAAGAGGA GCTGGACACT CAGACGAGCA GTAGGGGAAG CATTAGTCTC
    AGTGGAATAT TCTCTGATGC CAGGAATAGT GTTTTTGCTC CTTACGTACA TGTGGAGAAA
    AAGATGGAAG AAATTGGGGC AGTCTGTACA ATAATTAACG CAGAGGATGA AAAGTCAAAA
    GGTGGAGGCA AACCTGGTCA CACGGGGGTT GATGGCCCTT TAAACACCCT GCTGCCATCT
    CAGCTTGTGA GAAAAGAAAA GGAGAATGAA AAATCAAAAG AGAACTGGGT TTCAGAGCAA
    GTTGATTCAA CCTTGGAGTC AGGGAAAGCA CTTCCTACAT CCGAGTATGT TCTGCCAGGG
    CCTGTGATAT CCGAAACTGG ACACAGCGGT CACCTGTTGT GCTGCCTTTG TCAGAAATGG
    GCCAACTACA AACATCTTGG AGATCTCTAT GGTCCTTTTT ACCCAGCCGA GTATGCAGCC
    AAGCTCCCTA AGAATCAACC TCAAGTCCGA CAAACTTTGT CCCATTTTGG GCCAGCTGCC
    ACAGGTATGA CATCCATTCC AACAGAAGCG ATTCCCCAGG ATACAAGATT ACAAGAACCT
    CAGAATGTCA AGTCATCTGC AGAAAGCGAT TGCACAGTAA GCCAGGCAAC AAACACAACA
    TCCCCAGCCA CTATCATTGG CACTGTTTCT CCAATGCCTT TGGAAATGCC TTTCCAGAAT
    GCTATAGCCA GCAGCTCTAC TGCAAGGGTA ACAACTCATG CTTGGGATAC GGCAGCCGAG
    CTGGAAAGTG GACTGGGTAC ATCAAATTTA CAAGAAACAG ACGCTGAAAT CGCATCAAAG
    CAGTTGCAGG TAGAAAGTTC ACAGCAAAGG CCCCAGCACA GAAAACTGAC ATCTCATCCA
    CGCTTCAAAA GAAGACACAA ATCCAGCGAA GATTTGCCTC GAACTGTCCC AATTAACAGC
    AAAGCCTCCC TGCCCTTCCA GCCTCCACCC CCAAGCCTGG ATGCTCTGGG CCCCATGGCT
    CAGCTGGCGC AGCTCCCGCT GGTGCCTCTG GACCCTGAGG AGCTGTGGGT GCACGAGAGC
    TGCATGGTGT GGACCAGTGG CATATACCTG GTCAATGGGA GACTCTATGG CCTTCAGGAA
    GCACTGGATG GTGCTAGAGA TGCGAGCTGC TCTCATTGTG AGATGGCGGG CTCCACCCTC
    GGCTGCTACA GTAAAGGCTG CACGCTGAGG TACCACTACA TTTGTGCCAT AGACGCAGAT
    TGCTCTCTTA ACGAAGACAA CTTTTCTTTG AGGTGTCCAA AGCACAAGGG CAACAGGATG
    GCCAAGCCTG GAGTGGTGTA TCTGGAGCAG TCGGAGCGCG GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.