LOC100177345 cDNA ORF clone, Ciona intestinalis(vase tunicate)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100177345
Entrez Gene ID	100177345
Full Name	probable ATP-dependent RNA helicase DDX10
Gene Type	protein-coding
Organism	Ciona intestinalis(vase tunicate)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009864186.2	XP_009862488.2	probable ATP-dependent RNA helicase DDX10
XM_026836583.1	XP_026692384.1	probable ATP-dependent RNA helicase DDX10

Pathways from BioSystems
Gene Ontology

The following LOC100177345 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100177345 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCi17889	XM_026836583.1 Latest version!	Ciona intestinalis probable ATP-dependent RNA helicase DDX10 (LOC100177345), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCi17889	XM_009864186.2 Latest version!	Ciona intestinalis probable ATP-dependent RNA helicase DDX10 (LOC100177345), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100177345 ( XM_009864186.2 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_009862488.2 Ciona intestinalis probable ATP-dependent RNA helicase DDX10 (LOC100177345), mRNA.

Price & Availability↑

CloneID	OCi17889
Clone ID Related Accession (Same CDS sequence)	XM_009864186.2 , XM_026836583.1
Accession Version	XM_009864186.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-20
Organism	Ciona intestinalis(vase tunicate)
Product	probable ATP-dependent RNA helicase DDX10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020166.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 21, 2016 this sequence version replaced XM_009864186.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ciona intestinalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGTTC AACCTTCAGA CATTCAGAAC TTTAAAGCTT GGAAAGGAAA GAAAAGAAAA 
AGGAAATTTG TGAAGAAACC AAAGAAAGAA ATTGAAAAAG AGGAAACTGT GAGCTTGGTT 
GATCTTGATA CGACAAAAGT TGAAAAGTTT GAAGAATTTC CTTTTGCTAC AAAAACTAAG 
GCAGGTTTGC GAAAAGCTGG TTTTGTGTCA CTAACTGCAA TTCAGCGAGC TGCTATTAAA 
CCTGCGATGC TTGGGAAAGA TATTTTAGGT GCAGCGAAAA CTGGCTCAGG CAAAACATTA 
GCGTTCATTA TTCCAATATT AGAATGTTTG CATAAACATA ACTGGAATTC GTTGGATGGC 
CCAGGCGCTT TGGTGATTTC CCCAACACGA GAATTGGCGT TTCAAATATT TGAAGTTTTG 
AAGAAAGTTG GCGGGAAACA TAATTTTTCA GCTGGTTTGC TTATTGGTGG GAATAATGTG 
AAAGAGGAAG CTCATAGCGT AGGAAAAACT AATATTATAA TCTGCACACC TGGGCGCTTA 
TTGCAGCATA TGGACACTAC CTCTTATTTT CATATGAATA ACTTGAAAAT GTTAATACTG 
GATGAAGCGG ACAGGATATT GGACATGGGT TTTAAAACCA CGTTGGATGC AATCATTGAA 
AACCTTCCTA GTGAGCGGCA AACTTTGTTA TTTTCTGCAA CGCAAACGAA ATCTGTTAAG 
GACCTTGCCC GTTTAAGTTT GCGGGACCCT GCCTATATTT CTGTTCATTC AGAAGCAAAA 
CATTCAACTC CTCAGGGTTT AACACAAAGG TTTATCTGCT GTGAGCTTAA AGATAAATTG 
AATGTCTTGT TCTCATTTAT AAGAAACCAC CAAAAATCAA AATGCCTGGT GTTTGTGAGC 
AGCTGTAAGC AGGTACAGTT TATATTTGCT GCATTCTGTA AGCTGCGCCC AGGCACACCG 
ATGCTACATT TACATGGCCG CATGAACCAA CTGCGCAGAA TGTCAGTTTA CCAAGAGTTC 
TGCCGTAAAA AGTTTGCAGT TTTAGTGGCG ACAGACATCG CAGCACGAGG TTTAGATTTT 
CCAGAGATTG ACTGGGTAGT ACAACTCGAT TGTCCTGAAG ATGCCGACAC TTATATCCAC 
AGAGCCGGAC GTACAGCTCG ATATAACGGT AATGGGAACT CTCTCCTTGT GCTGACGCCC 
ACAGAGAAAG AAGCAATGCT GAAACATCTT CAAAACAAGA AAGTTCCGAT TTTGAAGTCA 
GAAATCCAGT CAAACAAACT TGGTAAAATT GACAGAAAAC TTCAATCTTA TTGTGCAGAG 
GATAAAGAAT TTAAAGAGAA AGCACAAAGA TGTTTTGTAG CGTATATAAG ATCCACATTT 
CTAATGAAAG ATAAATCAGT GTTTGATGCA ATGAAGGTCC CATTTAGTGA ATTCTCAAGC 
TCTCTTGGTC TTGCTATTCC ACCTAGAGTT AGGTTTTTAG AACGGAAAAT GAAATCTCTA 
AAAATGAAGG AAAATTCTGC TGTAAATGAT GAAACGAAAT CTTCTGAGTT TAACTTTAAT 
GAGATCGATG ATGAATGTGA TCTTATTACT GTAAAGAGGA AGAATGTATT TGAAGAGGAG 
GAAGAGACTT CTGAGGTGGA GGAGTTTGCT CCGGATGTTG CATTGGATCC TGCGAAGAAG 
AAAAGATCGA AAGCTGCGGA AGCGCGGAGG TTGTTGAGAA AGGGGATGCG AATGAATCAG 
AAGATTAAGT TTGGGGACGA AGGCGAGGTT GCCGAAGCGT TTCCTCCACT GCCTCCTAGT 
GGTGAGGATG GAGATGGTAT TAACATTGAG GAAGCAGCGA AAGCGATGCA GGAGGCGGAT 
GTAAAGGATC GTGAAAAACA TCGGTTGAGG ATCAGGGAGC GGCATCGTAA GAAGAGGTTG 
GAAGCCAAGA AGGAGAGAAG AAGATTGCAA GCTGAGTTGG AAGGGGGGAA ACAAGTTGTT 
ATTGGGGGTG ATGATGACAT AGGTGATGAT GACATAGGTG ATGATGTCAT AGGTGATGAT 
GTCATGGGTG ATGATGTGAT AGATGATGAT GATGTCACAA ACACTGTTGC ATACTCGGAT 
GAGGAGCAAG AAGAAGAGCA AGAGGCAAAA CACCGGAAGT TGAATACTGG GTTTGGAAGC 
AGTTTAGATG AAGATGAAAT GCTTGCTTTG CATTTGTTAA GAAATAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009862488.2
CDS	35..2245
Translation	MSVQPSDIQNFKAWKGKKRKRKFVKKPKKEIEKEETVSLVDLDTTKVEKFEEFPFATKTKAGLRKAGFVSLTAIQRAAIKPAMLGKDILGAAKTGSGKTLAFIIPILECLHKHNWNSLDGPGALVISPTRELAFQIFEVLKKVGGKHNFSAGLLIGGNNVKEEAHSVGKTNIIICTPGRLLQHMDTTSYFHMNNLKMLILDEADRILDMGFKTTLDAIIENLPSERQTLLFSATQTKSVKDLARLSLRDPAYISVHSEAKHSTPQGLTQRFICCELKDKLNVLFSFIRNHQKSKCLVFVSSCKQVQFIFAAFCKLRPGTPMLHLHGRMNQLRRMSVYQEFCRKKFAVLVATDIAARGLDFPEIDWVVQLDCPEDADTYIHRAGRTARYNGNGNSLLVLTPTEKEAMLKHLQNKKVPILKSEIQSNKLGKIDRKLQSYCAEDKEFKEKAQRCFVAYIRSTFLMKDKSVFDAMKVPFSEFSSSLGLAIPPRVRFLERKMKSLKMKENSAVNDETKSSEFNFNEIDDECDLITVKRKNVFEEEEETSEVEEFAPDVALDPAKKKRSKAAEARRLLRKGMRMNQKIKFGDEGEVAEAFPPLPPSGEDGDGINIEEAAKAMQEADVKDREKHRLRIRERHRKKRLEAKKERRRLQAELEGGKQVVIGGDDDIGDDDIGDDVIGDDVMGDDVIDDDDVTNTVAYSDEEQEEEQEAKHRKLNTGFGSSLDEDEMLALHLLRNK

Target ORF information:

RefSeq Version	XM_009864186.2
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis probable ATP-dependent RNA helicase DDX10 (LOC100177345), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009864186.2

ORF Insert Sequence:

ATGTCGGTTC AACCTTCAGA CATTCAGAAC TTTAAAGCTT GGAAAGGAAA GAAAAGAAAA 
AGGAAATTTG TGAAGAAACC AAAGAAAGAA ATTGAAAAAG AGGAAACTGT GAGCTTGGTT 
GATCTTGATA CGACAAAAGT TGAAAAGTTT GAAGAATTTC CTTTTGCTAC AAAAACTAAG 
GCAGGTTTGC GAAAAGCTGG TTTTGTGTCA CTAACTGCAA TTCAGCGAGC TGCTATTAAA 
CCTGCGATGC TTGGGAAAGA TATTTTAGGT GCAGCGAAAA CTGGCTCAGG CAAAACATTA 
GCGTTCATTA TTCCAATATT AGAATGTTTG CATAAACATA ACTGGAATTC GTTGGATGGC 
CCAGGCGCTT TGGTGATTTC CCCAACACGA GAATTGGCGT TTCAAATATT TGAAGTTTTG 
AAGAAAGTTG GCGGGAAACA TAATTTTTCA GCTGGTTTGC TTATTGGTGG GAATAATGTG 
AAAGAGGAAG CTCATAGCGT AGGAAAAACT AATATTATAA TCTGCACACC TGGGCGCTTA 
TTGCAGCATA TGGACACTAC CTCTTATTTT CATATGAATA ACTTGAAAAT GTTAATACTG 
GATGAAGCGG ACAGGATATT GGACATGGGT TTTAAAACCA CGTTGGATGC AATCATTGAA 
AACCTTCCTA GTGAGCGGCA AACTTTGTTA TTTTCTGCAA CGCAAACGAA ATCTGTTAAG 
GACCTTGCCC GTTTAAGTTT GCGGGACCCT GCCTATATTT CTGTTCATTC AGAAGCAAAA 
CATTCAACTC CTCAGGGTTT AACACAAAGG TTTATCTGCT GTGAGCTTAA AGATAAATTG 
AATGTCTTGT TCTCATTTAT AAGAAACCAC CAAAAATCAA AATGCCTGGT GTTTGTGAGC 
AGCTGTAAGC AGGTACAGTT TATATTTGCT GCATTCTGTA AGCTGCGCCC AGGCACACCG 
ATGCTACATT TACATGGCCG CATGAACCAA CTGCGCAGAA TGTCAGTTTA CCAAGAGTTC 
TGCCGTAAAA AGTTTGCAGT TTTAGTGGCG ACAGACATCG CAGCACGAGG TTTAGATTTT 
CCAGAGATTG ACTGGGTAGT ACAACTCGAT TGTCCTGAAG ATGCCGACAC TTATATCCAC 
AGAGCCGGAC GTACAGCTCG ATATAACGGT AATGGGAACT CTCTCCTTGT GCTGACGCCC 
ACAGAGAAAG AAGCAATGCT GAAACATCTT CAAAACAAGA AAGTTCCGAT TTTGAAGTCA 
GAAATCCAGT CAAACAAACT TGGTAAAATT GACAGAAAAC TTCAATCTTA TTGTGCAGAG 
GATAAAGAAT TTAAAGAGAA AGCACAAAGA TGTTTTGTAG CGTATATAAG ATCCACATTT 
CTAATGAAAG ATAAATCAGT GTTTGATGCA ATGAAGGTCC CATTTAGTGA ATTCTCAAGC 
TCTCTTGGTC TTGCTATTCC ACCTAGAGTT AGGTTTTTAG AACGGAAAAT GAAATCTCTA 
AAAATGAAGG AAAATTCTGC TGTAAATGAT GAAACGAAAT CTTCTGAGTT TAACTTTAAT 
GAGATCGATG ATGAATGTGA TCTTATTACT GTAAAGAGGA AGAATGTATT TGAAGAGGAG 
GAAGAGACTT CTGAGGTGGA GGAGTTTGCT CCGGATGTTG CATTGGATCC TGCGAAGAAG 
AAAAGATCGA AAGCTGCGGA AGCGCGGAGG TTGTTGAGAA AGGGGATGCG AATGAATCAG 
AAGATTAAGT TTGGGGACGA AGGCGAGGTT GCCGAAGCGT TTCCTCCACT GCCTCCTAGT 
GGTGAGGATG GAGATGGTAT TAACATTGAG GAAGCAGCGA AAGCGATGCA GGAGGCGGAT 
GTAAAGGATC GTGAAAAACA TCGGTTGAGG ATCAGGGAGC GGCATCGTAA GAAGAGGTTG 
GAAGCCAAGA AGGAGAGAAG AAGATTGCAA GCTGAGTTGG AAGGGGGGAA ACAAGTTGTT 
ATTGGGGGTG ATGATGACAT AGGTGATGAT GACATAGGTG ATGATGTCAT AGGTGATGAT 
GTCATGGGTG ATGATGTGAT AGATGATGAT GATGTCACAA ACACTGTTGC ATACTCGGAT 
GAGGAGCAAG AAGAAGAGCA AGAGGCAAAA CACCGGAAGT TGAATACTGG GTTTGGAAGC 
AGTTTAGATG AAGATGAAAT GCTTGCTTTG CATTTGTTAA GAAATAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100177345 ( XM_026836583.1 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_026692384.1 Ciona intestinalis probable ATP-dependent RNA helicase DDX10 (LOC100177345), mRNA.

Price & Availability↑

CloneID	OCi17889
Clone ID Related Accession (Same CDS sequence)	XM_009864186.2 , XM_026836583.1
Accession Version	XM_026836583.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-21
Organism	Ciona intestinalis(vase tunicate)
Product	probable ATP-dependent RNA helicase DDX10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020166.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ciona intestinalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGTTC AACCTTCAGA CATTCAGAAC TTTAAAGCTT GGAAAGGAAA GAAAAGAAAA 
AGGAAATTTG TGAAGAAACC AAAGAAAGAA ATTGAAAAAG AGGAAACTGT GAGCTTGGTT 
GATCTTGATA CGACAAAAGT TGAAAAGTTT GAAGAATTTC CTTTTGCTAC AAAAACTAAG 
GCAGGTTTGC GAAAAGCTGG TTTTGTGTCA CTAACTGCAA TTCAGCGAGC TGCTATTAAA 
CCTGCGATGC TTGGGAAAGA TATTTTAGGT GCAGCGAAAA CTGGCTCAGG CAAAACATTA 
GCGTTCATTA TTCCAATATT AGAATGTTTG CATAAACATA ACTGGAATTC GTTGGATGGC 
CCAGGCGCTT TGGTGATTTC CCCAACACGA GAATTGGCGT TTCAAATATT TGAAGTTTTG 
AAGAAAGTTG GCGGGAAACA TAATTTTTCA GCTGGTTTGC TTATTGGTGG GAATAATGTG 
AAAGAGGAAG CTCATAGCGT AGGAAAAACT AATATTATAA TCTGCACACC TGGGCGCTTA 
TTGCAGCATA TGGACACTAC CTCTTATTTT CATATGAATA ACTTGAAAAT GTTAATACTG 
GATGAAGCGG ACAGGATATT GGACATGGGT TTTAAAACCA CGTTGGATGC AATCATTGAA 
AACCTTCCTA GTGAGCGGCA AACTTTGTTA TTTTCTGCAA CGCAAACGAA ATCTGTTAAG 
GACCTTGCCC GTTTAAGTTT GCGGGACCCT GCCTATATTT CTGTTCATTC AGAAGCAAAA 
CATTCAACTC CTCAGGGTTT AACACAAAGG TTTATCTGCT GTGAGCTTAA AGATAAATTG 
AATGTCTTGT TCTCATTTAT AAGAAACCAC CAAAAATCAA AATGCCTGGT GTTTGTGAGC 
AGCTGTAAGC AGGTACAGTT TATATTTGCT GCATTCTGTA AGCTGCGCCC AGGCACACCG 
ATGCTACATT TACATGGCCG CATGAACCAA CTGCGCAGAA TGTCAGTTTA CCAAGAGTTC 
TGCCGTAAAA AGTTTGCAGT TTTAGTGGCG ACAGACATCG CAGCACGAGG TTTAGATTTT 
CCAGAGATTG ACTGGGTAGT ACAACTCGAT TGTCCTGAAG ATGCCGACAC TTATATCCAC 
AGAGCCGGAC GTACAGCTCG ATATAACGGT AATGGGAACT CTCTCCTTGT GCTGACGCCC 
ACAGAGAAAG AAGCAATGCT GAAACATCTT CAAAACAAGA AAGTTCCGAT TTTGAAGTCA 
GAAATCCAGT CAAACAAACT TGGTAAAATT GACAGAAAAC TTCAATCTTA TTGTGCAGAG 
GATAAAGAAT TTAAAGAGAA AGCACAAAGA TGTTTTGTAG CGTATATAAG ATCCACATTT 
CTAATGAAAG ATAAATCAGT GTTTGATGCA ATGAAGGTCC CATTTAGTGA ATTCTCAAGC 
TCTCTTGGTC TTGCTATTCC ACCTAGAGTT AGGTTTTTAG AACGGAAAAT GAAATCTCTA 
AAAATGAAGG AAAATTCTGC TGTAAATGAT GAAACGAAAT CTTCTGAGTT TAACTTTAAT 
GAGATCGATG ATGAATGTGA TCTTATTACT GTAAAGAGGA AGAATGTATT TGAAGAGGAG 
GAAGAGACTT CTGAGGTGGA GGAGTTTGCT CCGGATGTTG CATTGGATCC TGCGAAGAAG 
AAAAGATCGA AAGCTGCGGA AGCGCGGAGG TTGTTGAGAA AGGGGATGCG AATGAATCAG 
AAGATTAAGT TTGGGGACGA AGGCGAGGTT GCCGAAGCGT TTCCTCCACT GCCTCCTAGT 
GGTGAGGATG GAGATGGTAT TAACATTGAG GAAGCAGCGA AAGCGATGCA GGAGGCGGAT 
GTAAAGGATC GTGAAAAACA TCGGTTGAGG ATCAGGGAGC GGCATCGTAA GAAGAGGTTG 
GAAGCCAAGA AGGAGAGAAG AAGATTGCAA GCTGAGTTGG AAGGGGGGAA ACAAGTTGTT 
ATTGGGGGTG ATGATGACAT AGGTGATGAT GACATAGGTG ATGATGTCAT AGGTGATGAT 
GTCATGGGTG ATGATGTGAT AGATGATGAT GATGTCACAA ACACTGTTGC ATACTCGGAT 
GAGGAGCAAG AAGAAGAGCA AGAGGCAAAA CACCGGAAGT TGAATACTGG GTTTGGAAGC 
AGTTTAGATG AAGATGAAAT GCTTGCTTTG CATTTGTTAA GAAATAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026692384.1
CDS	35..2245
Translation	MSVQPSDIQNFKAWKGKKRKRKFVKKPKKEIEKEETVSLVDLDTTKVEKFEEFPFATKTKAGLRKAGFVSLTAIQRAAIKPAMLGKDILGAAKTGSGKTLAFIIPILECLHKHNWNSLDGPGALVISPTRELAFQIFEVLKKVGGKHNFSAGLLIGGNNVKEEAHSVGKTNIIICTPGRLLQHMDTTSYFHMNNLKMLILDEADRILDMGFKTTLDAIIENLPSERQTLLFSATQTKSVKDLARLSLRDPAYISVHSEAKHSTPQGLTQRFICCELKDKLNVLFSFIRNHQKSKCLVFVSSCKQVQFIFAAFCKLRPGTPMLHLHGRMNQLRRMSVYQEFCRKKFAVLVATDIAARGLDFPEIDWVVQLDCPEDADTYIHRAGRTARYNGNGNSLLVLTPTEKEAMLKHLQNKKVPILKSEIQSNKLGKIDRKLQSYCAEDKEFKEKAQRCFVAYIRSTFLMKDKSVFDAMKVPFSEFSSSLGLAIPPRVRFLERKMKSLKMKENSAVNDETKSSEFNFNEIDDECDLITVKRKNVFEEEEETSEVEEFAPDVALDPAKKKRSKAAEARRLLRKGMRMNQKIKFGDEGEVAEAFPPLPPSGEDGDGINIEEAAKAMQEADVKDREKHRLRIRERHRKKRLEAKKERRRLQAELEGGKQVVIGGDDDIGDDDIGDDVIGDDVMGDDVIDDDDVTNTVAYSDEEQEEEQEAKHRKLNTGFGSSLDEDEMLALHLLRNK

Target ORF information:

RefSeq Version	XM_026836583.1
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis probable ATP-dependent RNA helicase DDX10 (LOC100177345), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026836583.1

ORF Insert Sequence:

ATGTCGGTTC AACCTTCAGA CATTCAGAAC TTTAAAGCTT GGAAAGGAAA GAAAAGAAAA 
AGGAAATTTG TGAAGAAACC AAAGAAAGAA ATTGAAAAAG AGGAAACTGT GAGCTTGGTT 
GATCTTGATA CGACAAAAGT TGAAAAGTTT GAAGAATTTC CTTTTGCTAC AAAAACTAAG 
GCAGGTTTGC GAAAAGCTGG TTTTGTGTCA CTAACTGCAA TTCAGCGAGC TGCTATTAAA 
CCTGCGATGC TTGGGAAAGA TATTTTAGGT GCAGCGAAAA CTGGCTCAGG CAAAACATTA 
GCGTTCATTA TTCCAATATT AGAATGTTTG CATAAACATA ACTGGAATTC GTTGGATGGC 
CCAGGCGCTT TGGTGATTTC CCCAACACGA GAATTGGCGT TTCAAATATT TGAAGTTTTG 
AAGAAAGTTG GCGGGAAACA TAATTTTTCA GCTGGTTTGC TTATTGGTGG GAATAATGTG 
AAAGAGGAAG CTCATAGCGT AGGAAAAACT AATATTATAA TCTGCACACC TGGGCGCTTA 
TTGCAGCATA TGGACACTAC CTCTTATTTT CATATGAATA ACTTGAAAAT GTTAATACTG 
GATGAAGCGG ACAGGATATT GGACATGGGT TTTAAAACCA CGTTGGATGC AATCATTGAA 
AACCTTCCTA GTGAGCGGCA AACTTTGTTA TTTTCTGCAA CGCAAACGAA ATCTGTTAAG 
GACCTTGCCC GTTTAAGTTT GCGGGACCCT GCCTATATTT CTGTTCATTC AGAAGCAAAA 
CATTCAACTC CTCAGGGTTT AACACAAAGG TTTATCTGCT GTGAGCTTAA AGATAAATTG 
AATGTCTTGT TCTCATTTAT AAGAAACCAC CAAAAATCAA AATGCCTGGT GTTTGTGAGC 
AGCTGTAAGC AGGTACAGTT TATATTTGCT GCATTCTGTA AGCTGCGCCC AGGCACACCG 
ATGCTACATT TACATGGCCG CATGAACCAA CTGCGCAGAA TGTCAGTTTA CCAAGAGTTC 
TGCCGTAAAA AGTTTGCAGT TTTAGTGGCG ACAGACATCG CAGCACGAGG TTTAGATTTT 
CCAGAGATTG ACTGGGTAGT ACAACTCGAT TGTCCTGAAG ATGCCGACAC TTATATCCAC 
AGAGCCGGAC GTACAGCTCG ATATAACGGT AATGGGAACT CTCTCCTTGT GCTGACGCCC 
ACAGAGAAAG AAGCAATGCT GAAACATCTT CAAAACAAGA AAGTTCCGAT TTTGAAGTCA 
GAAATCCAGT CAAACAAACT TGGTAAAATT GACAGAAAAC TTCAATCTTA TTGTGCAGAG 
GATAAAGAAT TTAAAGAGAA AGCACAAAGA TGTTTTGTAG CGTATATAAG ATCCACATTT 
CTAATGAAAG ATAAATCAGT GTTTGATGCA ATGAAGGTCC CATTTAGTGA ATTCTCAAGC 
TCTCTTGGTC TTGCTATTCC ACCTAGAGTT AGGTTTTTAG AACGGAAAAT GAAATCTCTA 
AAAATGAAGG AAAATTCTGC TGTAAATGAT GAAACGAAAT CTTCTGAGTT TAACTTTAAT 
GAGATCGATG ATGAATGTGA TCTTATTACT GTAAAGAGGA AGAATGTATT TGAAGAGGAG 
GAAGAGACTT CTGAGGTGGA GGAGTTTGCT CCGGATGTTG CATTGGATCC TGCGAAGAAG 
AAAAGATCGA AAGCTGCGGA AGCGCGGAGG TTGTTGAGAA AGGGGATGCG AATGAATCAG 
AAGATTAAGT TTGGGGACGA AGGCGAGGTT GCCGAAGCGT TTCCTCCACT GCCTCCTAGT 
GGTGAGGATG GAGATGGTAT TAACATTGAG GAAGCAGCGA AAGCGATGCA GGAGGCGGAT 
GTAAAGGATC GTGAAAAACA TCGGTTGAGG ATCAGGGAGC GGCATCGTAA GAAGAGGTTG 
GAAGCCAAGA AGGAGAGAAG AAGATTGCAA GCTGAGTTGG AAGGGGGGAA ACAAGTTGTT 
ATTGGGGGTG ATGATGACAT AGGTGATGAT GACATAGGTG ATGATGTCAT AGGTGATGAT 
GTCATGGGTG ATGATGTGAT AGATGATGAT GATGTCACAA ACACTGTTGC ATACTCGGAT 
GAGGAGCAAG AAGAAGAGCA AGAGGCAAAA CACCGGAAGT TGAATACTGG GTTTGGAAGC 
AGTTTAGATG AAGATGAAAT GCTTGCTTTG CATTTGTTAA GAAATAAATA G