LOC100175761 cDNA ORF clone, Ciona intestinalis(vase tunicate)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100175761
Entrez Gene ID	100175761
Full Name	probable E3 ubiquitin-protein ligase HERC4
Synonyms	herc4
Gene Type	protein-coding
Organism	Ciona intestinalis(vase tunicate)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018811442.1	XP_018666987.1	probable E3 ubiquitin-protein ligase HERC4
XM_018811442.2	XP_018666987.1	probable E3 ubiquitin-protein ligase HERC4

Pathways from BioSystems
Gene Ontology

The following LOC100175761 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100175761 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCi70413	XM_018811442.2 Latest version!	Ciona intestinalis probable E3 ubiquitin-protein ligase HERC4 (LOC100175761), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OCi70413	XM_018811442.1	Ciona intestinalis probable E3 ubiquitin-protein ligase HERC4 (LOC100175761), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100175761 ( XM_018811442.1 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_018666987.1 Ciona intestinalis probable E3 ubiquitin-protein ligase HERC4 (LOC100175761), mRNA.

Price & Availability↑

CloneID	OCi70413
Clone ID Related Accession (Same CDS sequence)	XM_018811442.1 , XM_018811442.2
Accession Version	XM_018811442.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-20
Organism	Ciona intestinalis(vase tunicate)
Product	probable E3 ubiquitin-protein ligase HERC4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020166.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ciona intestinalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGCGT GGGGGAAAAG CATTATTGGG AAGTCATTTG GAAACGGGGC CAATCTGCAA 
GTGCCCACAG AAATAAACTG CTTTGGAAAA ACAATAATAA AATCAGTTCG AGTCAGCAGG 
ACAATTGCTG TGTTCCTACT CGAAGATGGT ACAGTTCATA CTTTCGGAGC CGAAAATGAC 
AACATTGGAC ATTCTGCGGG ATCGACCAAA GTTAGCGCCT TAGAAACTGT TCATATTGTG 
GATATTGCTC TTGGAGATTC CCATATACTA GCCATTGATG ACAAGGGAAA GGTATATGGG 
TGGGGTGGTA ATGATAAAGA TCAACTTGGG TGGACGGATG GCAACTGCTA TCCTTCCCCA 
AAGAAAATCA AATCGCTTTC AAATAAAATT GTCCAAGTAG CTTGTGGGCA TTCTCACAGT 
CTGTTTTTGG GCAGCAATGG AGCTTTATGG TCGGCTGGTA GTAATAACTA TGGTCAACTT 
GGTTTTGGTG ACAAATCCTC CACAGAACTT CGGCCATTGA TCTCACTTAA AGGGATTCCA 
TTTTCTCAGA TCTCTGCTGG ATTCTGGCAC AGTTTTTGTG TGACACATTC AGGGGCGGTG 
TTCTGCTGGG GTAGAAATGA TAATGGCCAG CTTGGTCTTG GTGATTGTGT GGATAGGAAT 
GTTCCAGTGC TGCTCAAAGC ATTGAGGTCC CTGTCTGTTA AGTACATATG CGCAGGAGAG 
CTACACACTG CAGCACTAAC CTTAGATGGT GGTGTGTTTA CCTTTGGCTC AAATCAGTTT 
GGTCAGCTTG GCCATGGGAG TTCCGGGCCT GAACCATTAC CTGTCTCCAC ACCAAGGAAA 
ATATTTGAGC TAATGGGTAA TGAGGTGACA CAAATTGCAT GCGGTGCCCA ACATACCGTT 
TGCTATGTTG GCAAAACAGG GAATGTTTAC ACTTTTGGGA ATAACAACCA TAGTCAGTTA 
GGGTATATAC CAAAACACCC AAGAAGTGAA TTTGTGCCTC ACCCTCTTTC AAGTGATATG 
TGGACAAGTA GTGCTTCAAC GTCATCTTAT CCCAAACTTA ATTCTGTGTT TGTAAAGGAA 
ATTGGTGCAG GGAAAACTGA GTCATTCATT TCAGTTTCAA CAAGACCAAA CTCATCTGAT 
TTTCGCCGAA TCCCGACCCA CCAGCAGATT TTTACTCTTT CTGACGAGTT TATCAGCAAA 
CTACAGAGCT TAGAAAACAC CAAGATCACT CCTGATGTTG CAAACTACCT TGAAACAATA 
ATGGGTTCAC AATCTTGCAT CAACGCATCT TTTCTTGCAA GCGACCACTA CAAAACCAGC 
AACAGTTATC ATGGAATAAG TTTAATGTCA GTTCGACTCC GATTTTCAAA GCTTTGCAAA 
TGCACTTGTA CAGATGTGCA GTCTATCATG GTAAAATCAA TGCAAAGTAG CTTGTTACCC 
AGTATAGCAA GAAGCCAACC AGATATTGAG ACATTACGGG TATTTGTTCT GCTTCCCGAA 
TGTGACTTGT TTACAAATTC AGAAAATTAC GGCAAAATCA CATTCCCATT TGCAAACCTT 
GTAATGTCAC TTGATGTTGC GCCTTCTAAG ATTCTGGATC GCTGGTACAG AGCAATGGAA 
CCCCTGTATT TTGCTCGCCC CATTGATATA TATAAGCAAT CCATTAAAAA TGTGTTAGAC 
CGCCCTCATT CTGTTGATGC GAGAGTAGCA GAGACAGGTG TACGCAGTGC TCTGGATTTT 
CTTGGTAAAT TAAACACTGT GAACAACACT GGTGAGGTGC CAATAGTTCC ATACCAAAGA 
TTCTACCTTC CAGAGATCAC TAAGCTCATA AACCTGGAAG ATGATTATGT TCGATGGTTA 
AGAGGCAATT CACAAGTTGT CATCTTCTGC AATTACCCGT TTTTATTGAA CTCGGTGGCC 
AAGTCTTCTC TGCTTCATAT TGATGCAGTG TGGCAGATGA GAGCTGCATA CAGTGAGGCA 
CAGGATAGGA ACATGGCTTC TTTATTTAGT TTCAGCACAC ATGCAATGCT AGAAACTCCT 
GTTTTGGAAC TGGAAGTGCG AAGGTCCGAC CTGCTACAAG ATGCATTGAA CCGCTTAGCT 
ATGGTGGATG TACGCAGCTT CAAAAAACCT TTAATGGTAA AGTTTGATGG GGAAGAAGGC 
CAGGATGAAG GTGGAGTTAG GAAAGAATTC TTCATGCTTA TATTGAAAGA GGTTCTTGAC 
CCTAAGTACG GGATGTTTCG CTTTTATGAT GAGTCTCATT TGATATGGTT TTCGGATTGG 
GAACTTGAAA GTGAGATGAT GTATTTCCTG GTTGGATTGA TATGTGGGCT CGCTATATAT 
AACGACACCA TCATCGATAT TTGCTTTCCG TTAGTTCTTT ATAAAAAACT GCTTGGTGAG 
CCACCTTTGT TTACAGATCT GGCAGAATTA GATCCAACAT TGTCACGAAG CATGAACCAT 
TTGTTGGAGT ACCAGAGTGA TGTCCATTCT ATTGAAGACA CATTCTGTTT GAACTTCACA 
GTGAGCCGCG ATAACTTTGG AGAAACGACA GAGGTCGATT TAATAGCTGG CGGATCCAAT 
ATTCCAGTCA CTGCGGACAA CAGAAAGGAA TATGTTCAGA GTTACATCAA CCACTTGTTC 
AATACATCCG TACAAAGGAA ATTTGAAGCT TTTAATAAAG GTTTCCACAA AGTTTGCGGC 
GGAAAAATCC TCCAGTTATT TCGTGCAACT GAGCTGATGG AAATGGTGGT CGGAAACCAG 
AACTATAATT GGGAGGAATT TGAAAAGTCA GCAGATTACA AGGGGGAGTA TTATCGCCAA 
CATCCAGTAA TCAGAATCTT CTGGAGGGTC TTCCACAAGT TTTCACTCGA GGAGAAAAAA 
GATTTTCTTC TTTTTCTTAC AGGTTCGAAC AAAATTCCGA TAACAGGCGT GAAAATTATC 
ATCCAGCCTG TAAAAACCTC TGAGAACCAT TTACCAGTCG CACATACATG TTTTAATTTG 
CTTGACTTAC CGTACTATAC AACTGAGCAT ACAATGGACA TGAAACTAAA GCAAGCCATG 
TTAAATAATC AAGGGTTTTA TCTAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018666987.1
CDS	403..3492
Translation	MYAWGKSIIGKSFGNGANLQVPTEINCFGKTIIKSVRVSRTIAVFLLEDGTVHTFGAENDNIGHSAGSTKVSALETVHIVDIALGDSHILAIDDKGKVYGWGGNDKDQLGWTDGNCYPSPKKIKSLSNKIVQVACGHSHSLFLGSNGALWSAGSNNYGQLGFGDKSSTELRPLISLKGIPFSQISAGFWHSFCVTHSGAVFCWGRNDNGQLGLGDCVDRNVPVLLKALRSLSVKYICAGELHTAALTLDGGVFTFGSNQFGQLGHGSSGPEPLPVSTPRKIFELMGNEVTQIACGAQHTVCYVGKTGNVYTFGNNNHSQLGYIPKHPRSEFVPHPLSSDMWTSSASTSSYPKLNSVFVKEIGAGKTESFISVSTRPNSSDFRRIPTHQQIFTLSDEFISKLQSLENTKITPDVANYLETIMGSQSCINASFLASDHYKTSNSYHGISLMSVRLRFSKLCKCTCTDVQSIMVKSMQSSLLPSIARSQPDIETLRVFVLLPECDLFTNSENYGKITFPFANLVMSLDVAPSKILDRWYRAMEPLYFARPIDIYKQSIKNVLDRPHSVDARVAETGVRSALDFLGKLNTVNNTGEVPIVPYQRFYLPEITKLINLEDDYVRWLRGNSQVVIFCNYPFLLNSVAKSSLLHIDAVWQMRAAYSEAQDRNMASLFSFSTHAMLETPVLELEVRRSDLLQDALNRLAMVDVRSFKKPLMVKFDGEEGQDEGGVRKEFFMLILKEVLDPKYGMFRFYDESHLIWFSDWELESEMMYFLVGLICGLAIYNDTIIDICFPLVLYKKLLGEPPLFTDLAELDPTLSRSMNHLLEYQSDVHSIEDTFCLNFTVSRDNFGETTEVDLIAGGSNIPVTADNRKEYVQSYINHLFNTSVQRKFEAFNKGFHKVCGGKILQLFRATELMEMVVGNQNYNWEEFEKSADYKGEYYRQHPVIRIFWRVFHKFSLEEKKDFLLFLTGSNKIPITGVKIIIQPVKTSENHLPVAHTCFNLLDLPYYTTEHTMDMKLKQAMLNNQGFYLI

Target ORF information:

RefSeq Version	XM_018811442.1
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis probable E3 ubiquitin-protein ligase HERC4 (LOC100175761), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018811442.1

ORF Insert Sequence:

ATGTATGCGT GGGGGAAAAG CATTATTGGG AAGTCATTTG GAAACGGGGC CAATCTGCAA 
GTGCCCACAG AAATAAACTG CTTTGGAAAA ACAATAATAA AATCAGTTCG AGTCAGCAGG 
ACAATTGCTG TGTTCCTACT CGAAGATGGT ACAGTTCATA CTTTCGGAGC CGAAAATGAC 
AACATTGGAC ATTCTGCGGG ATCGACCAAA GTTAGCGCCT TAGAAACTGT TCATATTGTG 
GATATTGCTC TTGGAGATTC CCATATACTA GCCATTGATG ACAAGGGAAA GGTATATGGG 
TGGGGTGGTA ATGATAAAGA TCAACTTGGG TGGACGGATG GCAACTGCTA TCCTTCCCCA 
AAGAAAATCA AATCGCTTTC AAATAAAATT GTCCAAGTAG CTTGTGGGCA TTCTCACAGT 
CTGTTTTTGG GCAGCAATGG AGCTTTATGG TCGGCTGGTA GTAATAACTA TGGTCAACTT 
GGTTTTGGTG ACAAATCCTC CACAGAACTT CGGCCATTGA TCTCACTTAA AGGGATTCCA 
TTTTCTCAGA TCTCTGCTGG ATTCTGGCAC AGTTTTTGTG TGACACATTC AGGGGCGGTG 
TTCTGCTGGG GTAGAAATGA TAATGGCCAG CTTGGTCTTG GTGATTGTGT GGATAGGAAT 
GTTCCAGTGC TGCTCAAAGC ATTGAGGTCC CTGTCTGTTA AGTACATATG CGCAGGAGAG 
CTACACACTG CAGCACTAAC CTTAGATGGT GGTGTGTTTA CCTTTGGCTC AAATCAGTTT 
GGTCAGCTTG GCCATGGGAG TTCCGGGCCT GAACCATTAC CTGTCTCCAC ACCAAGGAAA 
ATATTTGAGC TAATGGGTAA TGAGGTGACA CAAATTGCAT GCGGTGCCCA ACATACCGTT 
TGCTATGTTG GCAAAACAGG GAATGTTTAC ACTTTTGGGA ATAACAACCA TAGTCAGTTA 
GGGTATATAC CAAAACACCC AAGAAGTGAA TTTGTGCCTC ACCCTCTTTC AAGTGATATG 
TGGACAAGTA GTGCTTCAAC GTCATCTTAT CCCAAACTTA ATTCTGTGTT TGTAAAGGAA 
ATTGGTGCAG GGAAAACTGA GTCATTCATT TCAGTTTCAA CAAGACCAAA CTCATCTGAT 
TTTCGCCGAA TCCCGACCCA CCAGCAGATT TTTACTCTTT CTGACGAGTT TATCAGCAAA 
CTACAGAGCT TAGAAAACAC CAAGATCACT CCTGATGTTG CAAACTACCT TGAAACAATA 
ATGGGTTCAC AATCTTGCAT CAACGCATCT TTTCTTGCAA GCGACCACTA CAAAACCAGC 
AACAGTTATC ATGGAATAAG TTTAATGTCA GTTCGACTCC GATTTTCAAA GCTTTGCAAA 
TGCACTTGTA CAGATGTGCA GTCTATCATG GTAAAATCAA TGCAAAGTAG CTTGTTACCC 
AGTATAGCAA GAAGCCAACC AGATATTGAG ACATTACGGG TATTTGTTCT GCTTCCCGAA 
TGTGACTTGT TTACAAATTC AGAAAATTAC GGCAAAATCA CATTCCCATT TGCAAACCTT 
GTAATGTCAC TTGATGTTGC GCCTTCTAAG ATTCTGGATC GCTGGTACAG AGCAATGGAA 
CCCCTGTATT TTGCTCGCCC CATTGATATA TATAAGCAAT CCATTAAAAA TGTGTTAGAC 
CGCCCTCATT CTGTTGATGC GAGAGTAGCA GAGACAGGTG TACGCAGTGC TCTGGATTTT 
CTTGGTAAAT TAAACACTGT GAACAACACT GGTGAGGTGC CAATAGTTCC ATACCAAAGA 
TTCTACCTTC CAGAGATCAC TAAGCTCATA AACCTGGAAG ATGATTATGT TCGATGGTTA 
AGAGGCAATT CACAAGTTGT CATCTTCTGC AATTACCCGT TTTTATTGAA CTCGGTGGCC 
AAGTCTTCTC TGCTTCATAT TGATGCAGTG TGGCAGATGA GAGCTGCATA CAGTGAGGCA 
CAGGATAGGA ACATGGCTTC TTTATTTAGT TTCAGCACAC ATGCAATGCT AGAAACTCCT 
GTTTTGGAAC TGGAAGTGCG AAGGTCCGAC CTGCTACAAG ATGCATTGAA CCGCTTAGCT 
ATGGTGGATG TACGCAGCTT CAAAAAACCT TTAATGGTAA AGTTTGATGG GGAAGAAGGC 
CAGGATGAAG GTGGAGTTAG GAAAGAATTC TTCATGCTTA TATTGAAAGA GGTTCTTGAC 
CCTAAGTACG GGATGTTTCG CTTTTATGAT GAGTCTCATT TGATATGGTT TTCGGATTGG 
GAACTTGAAA GTGAGATGAT GTATTTCCTG GTTGGATTGA TATGTGGGCT CGCTATATAT 
AACGACACCA TCATCGATAT TTGCTTTCCG TTAGTTCTTT ATAAAAAACT GCTTGGTGAG 
CCACCTTTGT TTACAGATCT GGCAGAATTA GATCCAACAT TGTCACGAAG CATGAACCAT 
TTGTTGGAGT ACCAGAGTGA TGTCCATTCT ATTGAAGACA CATTCTGTTT GAACTTCACA 
GTGAGCCGCG ATAACTTTGG AGAAACGACA GAGGTCGATT TAATAGCTGG CGGATCCAAT 
ATTCCAGTCA CTGCGGACAA CAGAAAGGAA TATGTTCAGA GTTACATCAA CCACTTGTTC 
AATACATCCG TACAAAGGAA ATTTGAAGCT TTTAATAAAG GTTTCCACAA AGTTTGCGGC 
GGAAAAATCC TCCAGTTATT TCGTGCAACT GAGCTGATGG AAATGGTGGT CGGAAACCAG 
AACTATAATT GGGAGGAATT TGAAAAGTCA GCAGATTACA AGGGGGAGTA TTATCGCCAA 
CATCCAGTAA TCAGAATCTT CTGGAGGGTC TTCCACAAGT TTTCACTCGA GGAGAAAAAA 
GATTTTCTTC TTTTTCTTAC AGGTTCGAAC AAAATTCCGA TAACAGGCGT GAAAATTATC 
ATCCAGCCTG TAAAAACCTC TGAGAACCAT TTACCAGTCG CACATACATG TTTTAATTTG 
CTTGACTTAC CGTACTATAC AACTGAGCAT ACAATGGACA TGAAACTAAA GCAAGCCATG 
TTAAATAATC AAGGGTTTTA TCTAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100175761 ( XM_018811442.2 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_018666987.1 Ciona intestinalis probable E3 ubiquitin-protein ligase HERC4 (LOC100175761), mRNA.

Price & Availability↑

CloneID	OCi70413
Clone ID Related Accession (Same CDS sequence)	XM_018811442.1 , XM_018811442.2
Accession Version	XM_018811442.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-21
Organism	Ciona intestinalis(vase tunicate)
Product	probable E3 ubiquitin-protein ligase HERC4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020166.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 22, 2018 this sequence version replaced XM_018811442.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ciona intestinalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGCGT GGGGGAAAAG CATTATTGGG AAGTCATTTG GAAACGGGGC CAATCTGCAA 
GTGCCCACAG AAATAAACTG CTTTGGAAAA ACAATAATAA AATCAGTTCG AGTCAGCAGG 
ACAATTGCTG TGTTCCTACT CGAAGATGGT ACAGTTCATA CTTTCGGAGC CGAAAATGAC 
AACATTGGAC ATTCTGCGGG ATCGACCAAA GTTAGCGCCT TAGAAACTGT TCATATTGTG 
GATATTGCTC TTGGAGATTC CCATATACTA GCCATTGATG ACAAGGGAAA GGTATATGGG 
TGGGGTGGTA ATGATAAAGA TCAACTTGGG TGGACGGATG GCAACTGCTA TCCTTCCCCA 
AAGAAAATCA AATCGCTTTC AAATAAAATT GTCCAAGTAG CTTGTGGGCA TTCTCACAGT 
CTGTTTTTGG GCAGCAATGG AGCTTTATGG TCGGCTGGTA GTAATAACTA TGGTCAACTT 
GGTTTTGGTG ACAAATCCTC CACAGAACTT CGGCCATTGA TCTCACTTAA AGGGATTCCA 
TTTTCTCAGA TCTCTGCTGG ATTCTGGCAC AGTTTTTGTG TGACACATTC AGGGGCGGTG 
TTCTGCTGGG GTAGAAATGA TAATGGCCAG CTTGGTCTTG GTGATTGTGT GGATAGGAAT 
GTTCCAGTGC TGCTCAAAGC ATTGAGGTCC CTGTCTGTTA AGTACATATG CGCAGGAGAG 
CTACACACTG CAGCACTAAC CTTAGATGGT GGTGTGTTTA CCTTTGGCTC AAATCAGTTT 
GGTCAGCTTG GCCATGGGAG TTCCGGGCCT GAACCATTAC CTGTCTCCAC ACCAAGGAAA 
ATATTTGAGC TAATGGGTAA TGAGGTGACA CAAATTGCAT GCGGTGCCCA ACATACCGTT 
TGCTATGTTG GCAAAACAGG GAATGTTTAC ACTTTTGGGA ATAACAACCA TAGTCAGTTA 
GGGTATATAC CAAAACACCC AAGAAGTGAA TTTGTGCCTC ACCCTCTTTC AAGTGATATG 
TGGACAAGTA GTGCTTCAAC GTCATCTTAT CCCAAACTTA ATTCTGTGTT TGTAAAGGAA 
ATTGGTGCAG GGAAAACTGA GTCATTCATT TCAGTTTCAA CAAGACCAAA CTCATCTGAT 
TTTCGCCGAA TCCCGACCCA CCAGCAGATT TTTACTCTTT CTGACGAGTT TATCAGCAAA 
CTACAGAGCT TAGAAAACAC CAAGATCACT CCTGATGTTG CAAACTACCT TGAAACAATA 
ATGGGTTCAC AATCTTGCAT CAACGCATCT TTTCTTGCAA GCGACCACTA CAAAACCAGC 
AACAGTTATC ATGGAATAAG TTTAATGTCA GTTCGACTCC GATTTTCAAA GCTTTGCAAA 
TGCACTTGTA CAGATGTGCA GTCTATCATG GTAAAATCAA TGCAAAGTAG CTTGTTACCC 
AGTATAGCAA GAAGCCAACC AGATATTGAG ACATTACGGG TATTTGTTCT GCTTCCCGAA 
TGTGACTTGT TTACAAATTC AGAAAATTAC GGCAAAATCA CATTCCCATT TGCAAACCTT 
GTAATGTCAC TTGATGTTGC GCCTTCTAAG ATTCTGGATC GCTGGTACAG AGCAATGGAA 
CCCCTGTATT TTGCTCGCCC CATTGATATA TATAAGCAAT CCATTAAAAA TGTGTTAGAC 
CGCCCTCATT CTGTTGATGC GAGAGTAGCA GAGACAGGTG TACGCAGTGC TCTGGATTTT 
CTTGGTAAAT TAAACACTGT GAACAACACT GGTGAGGTGC CAATAGTTCC ATACCAAAGA 
TTCTACCTTC CAGAGATCAC TAAGCTCATA AACCTGGAAG ATGATTATGT TCGATGGTTA 
AGAGGCAATT CACAAGTTGT CATCTTCTGC AATTACCCGT TTTTATTGAA CTCGGTGGCC 
AAGTCTTCTC TGCTTCATAT TGATGCAGTG TGGCAGATGA GAGCTGCATA CAGTGAGGCA 
CAGGATAGGA ACATGGCTTC TTTATTTAGT TTCAGCACAC ATGCAATGCT AGAAACTCCT 
GTTTTGGAAC TGGAAGTGCG AAGGTCCGAC CTGCTACAAG ATGCATTGAA CCGCTTAGCT 
ATGGTGGATG TACGCAGCTT CAAAAAACCT TTAATGGTAA AGTTTGATGG GGAAGAAGGC 
CAGGATGAAG GTGGAGTTAG GAAAGAATTC TTCATGCTTA TATTGAAAGA GGTTCTTGAC 
CCTAAGTACG GGATGTTTCG CTTTTATGAT GAGTCTCATT TGATATGGTT TTCGGATTGG 
GAACTTGAAA GTGAGATGAT GTATTTCCTG GTTGGATTGA TATGTGGGCT CGCTATATAT 
AACGACACCA TCATCGATAT TTGCTTTCCG TTAGTTCTTT ATAAAAAACT GCTTGGTGAG 
CCACCTTTGT TTACAGATCT GGCAGAATTA GATCCAACAT TGTCACGAAG CATGAACCAT 
TTGTTGGAGT ACCAGAGTGA TGTCCATTCT ATTGAAGACA CATTCTGTTT GAACTTCACA 
GTGAGCCGCG ATAACTTTGG AGAAACGACA GAGGTCGATT TAATAGCTGG CGGATCCAAT 
ATTCCAGTCA CTGCGGACAA CAGAAAGGAA TATGTTCAGA GTTACATCAA CCACTTGTTC 
AATACATCCG TACAAAGGAA ATTTGAAGCT TTTAATAAAG GTTTCCACAA AGTTTGCGGC 
GGAAAAATCC TCCAGTTATT TCGTGCAACT GAGCTGATGG AAATGGTGGT CGGAAACCAG 
AACTATAATT GGGAGGAATT TGAAAAGTCA GCAGATTACA AGGGGGAGTA TTATCGCCAA 
CATCCAGTAA TCAGAATCTT CTGGAGGGTC TTCCACAAGT TTTCACTCGA GGAGAAAAAA 
GATTTTCTTC TTTTTCTTAC AGGTTCGAAC AAAATTCCGA TAACAGGCGT GAAAATTATC 
ATCCAGCCTG TAAAAACCTC TGAGAACCAT TTACCAGTCG CACATACATG TTTTAATTTG 
CTTGACTTAC CGTACTATAC AACTGAGCAT ACAATGGACA TGAAACTAAA GCAAGCCATG 
TTAAATAATC AAGGGTTTTA TCTAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018666987.1
CDS	473..3562
Translation	MYAWGKSIIGKSFGNGANLQVPTEINCFGKTIIKSVRVSRTIAVFLLEDGTVHTFGAENDNIGHSAGSTKVSALETVHIVDIALGDSHILAIDDKGKVYGWGGNDKDQLGWTDGNCYPSPKKIKSLSNKIVQVACGHSHSLFLGSNGALWSAGSNNYGQLGFGDKSSTELRPLISLKGIPFSQISAGFWHSFCVTHSGAVFCWGRNDNGQLGLGDCVDRNVPVLLKALRSLSVKYICAGELHTAALTLDGGVFTFGSNQFGQLGHGSSGPEPLPVSTPRKIFELMGNEVTQIACGAQHTVCYVGKTGNVYTFGNNNHSQLGYIPKHPRSEFVPHPLSSDMWTSSASTSSYPKLNSVFVKEIGAGKTESFISVSTRPNSSDFRRIPTHQQIFTLSDEFISKLQSLENTKITPDVANYLETIMGSQSCINASFLASDHYKTSNSYHGISLMSVRLRFSKLCKCTCTDVQSIMVKSMQSSLLPSIARSQPDIETLRVFVLLPECDLFTNSENYGKITFPFANLVMSLDVAPSKILDRWYRAMEPLYFARPIDIYKQSIKNVLDRPHSVDARVAETGVRSALDFLGKLNTVNNTGEVPIVPYQRFYLPEITKLINLEDDYVRWLRGNSQVVIFCNYPFLLNSVAKSSLLHIDAVWQMRAAYSEAQDRNMASLFSFSTHAMLETPVLELEVRRSDLLQDALNRLAMVDVRSFKKPLMVKFDGEEGQDEGGVRKEFFMLILKEVLDPKYGMFRFYDESHLIWFSDWELESEMMYFLVGLICGLAIYNDTIIDICFPLVLYKKLLGEPPLFTDLAELDPTLSRSMNHLLEYQSDVHSIEDTFCLNFTVSRDNFGETTEVDLIAGGSNIPVTADNRKEYVQSYINHLFNTSVQRKFEAFNKGFHKVCGGKILQLFRATELMEMVVGNQNYNWEEFEKSADYKGEYYRQHPVIRIFWRVFHKFSLEEKKDFLLFLTGSNKIPITGVKIIIQPVKTSENHLPVAHTCFNLLDLPYYTTEHTMDMKLKQAMLNNQGFYLI

Target ORF information:

RefSeq Version	XM_018811442.2
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis probable E3 ubiquitin-protein ligase HERC4 (LOC100175761), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018811442.2

ORF Insert Sequence:

ATGTATGCGT GGGGGAAAAG CATTATTGGG AAGTCATTTG GAAACGGGGC CAATCTGCAA 
GTGCCCACAG AAATAAACTG CTTTGGAAAA ACAATAATAA AATCAGTTCG AGTCAGCAGG 
ACAATTGCTG TGTTCCTACT CGAAGATGGT ACAGTTCATA CTTTCGGAGC CGAAAATGAC 
AACATTGGAC ATTCTGCGGG ATCGACCAAA GTTAGCGCCT TAGAAACTGT TCATATTGTG 
GATATTGCTC TTGGAGATTC CCATATACTA GCCATTGATG ACAAGGGAAA GGTATATGGG 
TGGGGTGGTA ATGATAAAGA TCAACTTGGG TGGACGGATG GCAACTGCTA TCCTTCCCCA 
AAGAAAATCA AATCGCTTTC AAATAAAATT GTCCAAGTAG CTTGTGGGCA TTCTCACAGT 
CTGTTTTTGG GCAGCAATGG AGCTTTATGG TCGGCTGGTA GTAATAACTA TGGTCAACTT 
GGTTTTGGTG ACAAATCCTC CACAGAACTT CGGCCATTGA TCTCACTTAA AGGGATTCCA 
TTTTCTCAGA TCTCTGCTGG ATTCTGGCAC AGTTTTTGTG TGACACATTC AGGGGCGGTG 
TTCTGCTGGG GTAGAAATGA TAATGGCCAG CTTGGTCTTG GTGATTGTGT GGATAGGAAT 
GTTCCAGTGC TGCTCAAAGC ATTGAGGTCC CTGTCTGTTA AGTACATATG CGCAGGAGAG 
CTACACACTG CAGCACTAAC CTTAGATGGT GGTGTGTTTA CCTTTGGCTC AAATCAGTTT 
GGTCAGCTTG GCCATGGGAG TTCCGGGCCT GAACCATTAC CTGTCTCCAC ACCAAGGAAA 
ATATTTGAGC TAATGGGTAA TGAGGTGACA CAAATTGCAT GCGGTGCCCA ACATACCGTT 
TGCTATGTTG GCAAAACAGG GAATGTTTAC ACTTTTGGGA ATAACAACCA TAGTCAGTTA 
GGGTATATAC CAAAACACCC AAGAAGTGAA TTTGTGCCTC ACCCTCTTTC AAGTGATATG 
TGGACAAGTA GTGCTTCAAC GTCATCTTAT CCCAAACTTA ATTCTGTGTT TGTAAAGGAA 
ATTGGTGCAG GGAAAACTGA GTCATTCATT TCAGTTTCAA CAAGACCAAA CTCATCTGAT 
TTTCGCCGAA TCCCGACCCA CCAGCAGATT TTTACTCTTT CTGACGAGTT TATCAGCAAA 
CTACAGAGCT TAGAAAACAC CAAGATCACT CCTGATGTTG CAAACTACCT TGAAACAATA 
ATGGGTTCAC AATCTTGCAT CAACGCATCT TTTCTTGCAA GCGACCACTA CAAAACCAGC 
AACAGTTATC ATGGAATAAG TTTAATGTCA GTTCGACTCC GATTTTCAAA GCTTTGCAAA 
TGCACTTGTA CAGATGTGCA GTCTATCATG GTAAAATCAA TGCAAAGTAG CTTGTTACCC 
AGTATAGCAA GAAGCCAACC AGATATTGAG ACATTACGGG TATTTGTTCT GCTTCCCGAA 
TGTGACTTGT TTACAAATTC AGAAAATTAC GGCAAAATCA CATTCCCATT TGCAAACCTT 
GTAATGTCAC TTGATGTTGC GCCTTCTAAG ATTCTGGATC GCTGGTACAG AGCAATGGAA 
CCCCTGTATT TTGCTCGCCC CATTGATATA TATAAGCAAT CCATTAAAAA TGTGTTAGAC 
CGCCCTCATT CTGTTGATGC GAGAGTAGCA GAGACAGGTG TACGCAGTGC TCTGGATTTT 
CTTGGTAAAT TAAACACTGT GAACAACACT GGTGAGGTGC CAATAGTTCC ATACCAAAGA 
TTCTACCTTC CAGAGATCAC TAAGCTCATA AACCTGGAAG ATGATTATGT TCGATGGTTA 
AGAGGCAATT CACAAGTTGT CATCTTCTGC AATTACCCGT TTTTATTGAA CTCGGTGGCC 
AAGTCTTCTC TGCTTCATAT TGATGCAGTG TGGCAGATGA GAGCTGCATA CAGTGAGGCA 
CAGGATAGGA ACATGGCTTC TTTATTTAGT TTCAGCACAC ATGCAATGCT AGAAACTCCT 
GTTTTGGAAC TGGAAGTGCG AAGGTCCGAC CTGCTACAAG ATGCATTGAA CCGCTTAGCT 
ATGGTGGATG TACGCAGCTT CAAAAAACCT TTAATGGTAA AGTTTGATGG GGAAGAAGGC 
CAGGATGAAG GTGGAGTTAG GAAAGAATTC TTCATGCTTA TATTGAAAGA GGTTCTTGAC 
CCTAAGTACG GGATGTTTCG CTTTTATGAT GAGTCTCATT TGATATGGTT TTCGGATTGG 
GAACTTGAAA GTGAGATGAT GTATTTCCTG GTTGGATTGA TATGTGGGCT CGCTATATAT 
AACGACACCA TCATCGATAT TTGCTTTCCG TTAGTTCTTT ATAAAAAACT GCTTGGTGAG 
CCACCTTTGT TTACAGATCT GGCAGAATTA GATCCAACAT TGTCACGAAG CATGAACCAT 
TTGTTGGAGT ACCAGAGTGA TGTCCATTCT ATTGAAGACA CATTCTGTTT GAACTTCACA 
GTGAGCCGCG ATAACTTTGG AGAAACGACA GAGGTCGATT TAATAGCTGG CGGATCCAAT 
ATTCCAGTCA CTGCGGACAA CAGAAAGGAA TATGTTCAGA GTTACATCAA CCACTTGTTC 
AATACATCCG TACAAAGGAA ATTTGAAGCT TTTAATAAAG GTTTCCACAA AGTTTGCGGC 
GGAAAAATCC TCCAGTTATT TCGTGCAACT GAGCTGATGG AAATGGTGGT CGGAAACCAG 
AACTATAATT GGGAGGAATT TGAAAAGTCA GCAGATTACA AGGGGGAGTA TTATCGCCAA 
CATCCAGTAA TCAGAATCTT CTGGAGGGTC TTCCACAAGT TTTCACTCGA GGAGAAAAAA 
GATTTTCTTC TTTTTCTTAC AGGTTCGAAC AAAATTCCGA TAACAGGCGT GAAAATTATC 
ATCCAGCCTG TAAAAACCTC TGAGAACCAT TTACCAGTCG CACATACATG TTTTAATTTG 
CTTGACTTAC CGTACTATAC AACTGAGCAT ACAATGGACA TGAAACTAAA GCAAGCCATG 
TTAAATAATC AAGGGTTTTA TCTAATTTGA