klhl10 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	klhl10
Entrez Gene ID	3354863
Full Name	kelch like family member 10
Synonyms	CG12423,Dmel\CG12423,anon-WO0140519.152
General protein information	Preferred Names kelch like family member 10 Names CG12423 gene product from transcript CG12423-RB CG12423-PA CG12423-PB klhl10-PA klhl10-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001015100.2	NP_001015100.2	kelch like family member 10, isoform A
NM_001015101.2	NP_001015101.2	kelch like family member 10, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	KLHL10	XP_001167701.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003895	XP_308125.5
Danio rerio (zebrafish)	LOC100333629	XP_002665162.1
Xenopus tropicalis (tropical clawed frog)	klhl10	XP_002943971.2
Mus musculus (house mouse)	Klhl10	NP_080003.1
Rattus norvegicus (Norway rat)	Klhl10	NP_001001510.1
Homo sapiens (human)	KLHL10	NP_689680.2
Danio rerio (zebrafish)	LOC560378	XP_001920255.1
Danio rerio (zebrafish)	LOC560458	XP_688950.1
Drosophila melanogaster (fruit fly)	klhl10	NP_001015101.2
Canis lupus familiaris (dog)	KLHL10	XP_537641.2
Bos taurus (cattle)	KLHL10	NP_001071501.1
Gallus gallus (chicken)	KLHL10	NP_001264227.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following klhl10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the klhl10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00005	NM_001015101.2 Latest version!	Drosophila melanogaster kelch like family member 10 (klhl10), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa00005	NM_001015100.2 Latest version!	Drosophila melanogaster kelch like family member 10 (klhl10), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

klhl10 ( NM_001015100.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001015100.2 Drosophila melanogaster kelch like family member 10 (klhl10), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00005
Clone ID Related Accession (Same CDS sequence)	NM_001015100.2 , NM_001015101.2
Accession Version	NM_001015100.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kelch like family member 10, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NW_007931083). On Nov 20, 2007 this sequence version replaced NM_001015100.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTCGTA ATCAAAACGA AAGCAACCTC CTTGATATCA ATGAAGATCT GCCTTTTGCT 
CTTGCTCTGC CCCCCGTCTC AATGGATTCG TTGCAGCTGA ACGCAGACGG CAATAGCCCC 
GAGGACAAGA CGCTCGGTAT TGGCCGTAAA ACCATTTTGA ATGCGCACAC AAAATCTTTG 
GCTCCCATTG TGAGTGACGA GGCCCTGAAC ACGCTAAATG AGCTCCGCGA GCAAAATCTT 
CTCTGCGACG CCCAGATTTC TGTGGGGGAG GATGTGTTCA ACGTACATCG TGCAATAATG 
TGTTCATGTA GCTCATACTT TCGAGCTCAA TTCACAGGGT TTAACGCTGA CACTCCCGGA 
TGTGTAGATG GGTCAGATGC AAAAAAAAAC AATAATTTTA TTCATATCCC CGGCGTGAGT 
TCATGTATAA TGAACTGTGT AATACAGTAC GCTTACCTGC GTCAAACAAA CATCTCCGAG 
AGCAATGTTC ATGAGTTACT TATCTGCGCT GACTACGTTG GCATGGTAGG TTTAGTTAAG 
AAATGTAAAG ACTACCTCGG TCGCATACTC ACCCCCGAAA ACTGTGTCAG TATTATGGGA 
TTCGCACGGT TCCGATTTTT GGAGGACCTT CACCTGAAGG CCAGGAACTA CACTCTAAGG 
TACTTTACTG AAGTTGCATA TCGTAATATT GACATACTAG ATATGTCAGC TGAAGACTTT 
TACAGTATTA TTAGCGATGA TGAGTTAAAT ACCCGCGAAG AGGATCACGT ATGGAAATTG 
TGTGTTAAAT GGATCGACCG TAATCCAGAA AGCCGCAAGC GCCATGTTGC CCATCTAATG 
ACTGGGGTAC GACTGGGCCT AATGACTCCA AAGTGCTTCA TGGAAGAGGT TAAGGAACAC 
CCATACGTCT TAGAATGTGA AGCCGCTAAG CCACTGATCG TTGACACCTT TAAATTTATG 
TACGACCTGG ACTTTTTGAA TCCTCAGGCA GACGAGCTTA CCACTCCACC ACTGGCAATG 
CCAAGGCTAC CGCACGAAGT CATCTTTGCC ATAGGCGGCT GGAGCGGTGG CACCTCAAAA 
GGTTGCATCG AGACTTATGA CACGAGGGCA GATAGATGGG TAACCATTAA CGCAGAGGAT 
CCTGCTGGTC CCCGTGCGTA TCATGGAACC GCAGTGCTGG GTTTCAAAAT ATTCTCGATT 
GGCGGATATG ATGGCGTAGA GTATTTCAAC ACATGCCGAG TTTTTGACGC GGTAAAAAAA 
AAATGGAACG AAATCGCACC GATGCATTGC AGGCGCTGCT ACGTAAGTGT AACAGAGTTA 
AATGGAATGA TATATGCAAT CGGCGGCTAC GACGGGCATA ACCGGCTGAA CACTGTGGAG 
CGGTATAATC CAAGGACCAA CCAATGGTCC GTTATCCCAC CAATGAACAT GCAACGCTCT 
GATGCCAGCG CCTGTACTTT GCAAGAACGC ATATATGCTA CAGGGGGATT TAACGGCCAG 
GAATGCCTAG ACAGCGCCGA GTATTATGAC CCTGTAACCA ACGTTTGGAC TAGAATACCT 
AACATGAATC ATCGTCGGTC TGGAGTGAGC TGCGTGGCCT TTAGAAACCA GCTTTACGTT 
ATCGGCGGCT TTAACGGCAC AGCGCGTCTA TCTACCGGAG AACGCTTTGA TCCGGACACG 
CAAACTTGGC ACTTCATTCG TGAAATGAAC CACTCCCGCA GTAACTTCGG GCTAGAGATT 
ATTGACGATA TGATCTTCGC TATAGGCGGC TTTAACGGCG TCTCGACGAT CTCTCATACG 
GAATGCTATG TAGCCGAAAC AGACGAATGG ATGGAAGCCA CAGATATGAA TATTGTTCGT 
TCCGCCTTGT CTGCCAATAA CATAGCTGGG CTTCCAAACA AGCGAGACTA TATACACAAA 
GAGCGGGATC GCCTGATGGA GGAGCGGCGT CAGCGTCTTA TGGCAACCGC CATGGCTCGG 
GAGAATGTAG GACACGGCAA TGCCTCGCAG ATGTTAGTGG AACACAATGA CGGAGCCATG 
GACGACTACG AAAGTCCTGA TGAAGTAGAT GAGACTGATG AGCAAGAGCA ATCAGCACGC 
ATAAGCCGGA CTATACCACC CTCTGTAGGG CTTCCAAACC CAGCAGTTGC TGCTGAAACG 
ATACGAGATC AATCTCAATT GGTGTCCACT GCTCAACTTA ATAGAGTAAT GGAGGATCGC 
GGTATGGACA ACAATAGGAG CAATCGTGTG CAACTGCGTA ATCAACGCCA TGGCTCGCAT 
CATGAAATTC GTCGTCGTAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001015100.2
CDS	181..2484
Translation	MSRNQNESNLLDINEDLPFALALPPVSMDSLQLNADGNSPEDKTLGIGRKTILNAHTKSLAPIVSDEALNTLNELREQNLLCDAQISVGEDVFNVHRAIMCSCSSYFRAQFTGFNADTPGCVDGSDAKKNNNFIHIPGVSSCIMNCVIQYAYLRQTNISESNVHELLICADYVGMVGLVKKCKDYLGRILTPENCVSIMGFARFRFLEDLHLKARNYTLRYFTEVAYRNIDILDMSAEDFYSIISDDELNTREEDHVWKLCVKWIDRNPESRKRHVAHLMTGVRLGLMTPKCFMEEVKEHPYVLECEAAKPLIVDTFKFMYDLDFLNPQADELTTPPLAMPRLPHEVIFAIGGWSGGTSKGCIETYDTRADRWVTINAEDPAGPRAYHGTAVLGFKIFSIGGYDGVEYFNTCRVFDAVKKKWNEIAPMHCRRCYVSVTELNGMIYAIGGYDGHNRLNTVERYNPRTNQWSVIPPMNMQRSDASACTLQERIYATGGFNGQECLDSAEYYDPVTNVWTRIPNMNHRRSGVSCVAFRNQLYVIGGFNGTARLSTGERFDPDTQTWHFIREMNHSRSNFGLEIIDDMIFAIGGFNGVSTISHTECYVAETDEWMEATDMNIVRSALSANNIAGLPNKRDYIHKERDRLMEERRQRLMATAMARENVGHGNASQMLVEHNDGAMDDYESPDEVDETDEQEQSARISRTIPPSVGLPNPAVAAETIRDQSQLVSTAQLNRVMEDRGMDNNRSNRVQLRNQRHGSHHEIRRRT

Target ORF information:

RefSeq Version	NM_001015100.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kelch like family member 10 (klhl10), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001015100.2

ORF Insert Sequence:

ATGAGTCGTA ATCAAAACGA AAGCAACCTC CTTGATATCA ATGAAGATCT GCCTTTTGCT 
CTTGCTCTGC CCCCCGTCTC AATGGATTCG TTGCAGCTGA ACGCAGACGG CAATAGCCCC 
GAGGACAAGA CGCTCGGTAT TGGCCGTAAA ACCATTTTGA ATGCGCACAC AAAATCTTTG 
GCTCCCATTG TGAGTGACGA GGCCCTGAAC ACGCTAAATG AGCTCCGCGA GCAAAATCTT 
CTCTGCGACG CCCAGATTTC TGTGGGGGAG GATGTGTTCA ACGTACATCG TGCAATAATG 
TGTTCATGTA GCTCATACTT TCGAGCTCAA TTCACAGGGT TTAACGCTGA CACTCCCGGA 
TGTGTAGATG GGTCAGATGC AAAAAAAAAC AATAATTTTA TTCATATCCC CGGCGTGAGT 
TCATGTATAA TGAACTGTGT AATACAGTAC GCTTACCTGC GTCAAACAAA CATCTCCGAG 
AGCAATGTTC ATGAGTTACT TATCTGCGCT GACTACGTTG GCATGGTAGG TTTAGTTAAG 
AAATGTAAAG ACTACCTCGG TCGCATACTC ACCCCCGAAA ACTGTGTCAG TATTATGGGA 
TTCGCACGGT TCCGATTTTT GGAGGACCTT CACCTGAAGG CCAGGAACTA CACTCTAAGG 
TACTTTACTG AAGTTGCATA TCGTAATATT GACATACTAG ATATGTCAGC TGAAGACTTT 
TACAGTATTA TTAGCGATGA TGAGTTAAAT ACCCGCGAAG AGGATCACGT ATGGAAATTG 
TGTGTTAAAT GGATCGACCG TAATCCAGAA AGCCGCAAGC GCCATGTTGC CCATCTAATG 
ACTGGGGTAC GACTGGGCCT AATGACTCCA AAGTGCTTCA TGGAAGAGGT TAAGGAACAC 
CCATACGTCT TAGAATGTGA AGCCGCTAAG CCACTGATCG TTGACACCTT TAAATTTATG 
TACGACCTGG ACTTTTTGAA TCCTCAGGCA GACGAGCTTA CCACTCCACC ACTGGCAATG 
CCAAGGCTAC CGCACGAAGT CATCTTTGCC ATAGGCGGCT GGAGCGGTGG CACCTCAAAA 
GGTTGCATCG AGACTTATGA CACGAGGGCA GATAGATGGG TAACCATTAA CGCAGAGGAT 
CCTGCTGGTC CCCGTGCGTA TCATGGAACC GCAGTGCTGG GTTTCAAAAT ATTCTCGATT 
GGCGGATATG ATGGCGTAGA GTATTTCAAC ACATGCCGAG TTTTTGACGC GGTAAAAAAA 
AAATGGAACG AAATCGCACC GATGCATTGC AGGCGCTGCT ACGTAAGTGT AACAGAGTTA 
AATGGAATGA TATATGCAAT CGGCGGCTAC GACGGGCATA ACCGGCTGAA CACTGTGGAG 
CGGTATAATC CAAGGACCAA CCAATGGTCC GTTATCCCAC CAATGAACAT GCAACGCTCT 
GATGCCAGCG CCTGTACTTT GCAAGAACGC ATATATGCTA CAGGGGGATT TAACGGCCAG 
GAATGCCTAG ACAGCGCCGA GTATTATGAC CCTGTAACCA ACGTTTGGAC TAGAATACCT 
AACATGAATC ATCGTCGGTC TGGAGTGAGC TGCGTGGCCT TTAGAAACCA GCTTTACGTT 
ATCGGCGGCT TTAACGGCAC AGCGCGTCTA TCTACCGGAG AACGCTTTGA TCCGGACACG 
CAAACTTGGC ACTTCATTCG TGAAATGAAC CACTCCCGCA GTAACTTCGG GCTAGAGATT 
ATTGACGATA TGATCTTCGC TATAGGCGGC TTTAACGGCG TCTCGACGAT CTCTCATACG 
GAATGCTATG TAGCCGAAAC AGACGAATGG ATGGAAGCCA CAGATATGAA TATTGTTCGT 
TCCGCCTTGT CTGCCAATAA CATAGCTGGG CTTCCAAACA AGCGAGACTA TATACACAAA 
GAGCGGGATC GCCTGATGGA GGAGCGGCGT CAGCGTCTTA TGGCAACCGC CATGGCTCGG 
GAGAATGTAG GACACGGCAA TGCCTCGCAG ATGTTAGTGG AACACAATGA CGGAGCCATG 
GACGACTACG AAAGTCCTGA TGAAGTAGAT GAGACTGATG AGCAAGAGCA ATCAGCACGC 
ATAAGCCGGA CTATACCACC CTCTGTAGGG CTTCCAAACC CAGCAGTTGC TGCTGAAACG 
ATACGAGATC AATCTCAATT GGTGTCCACT GCTCAACTTA ATAGAGTAAT GGAGGATCGC 
GGTATGGACA ACAATAGGAG CAATCGTGTG CAACTGCGTA ATCAACGCCA TGGCTCGCAT 
CATGAAATTC GTCGTCGTAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

klhl10 ( NM_001015101.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001015101.2 Drosophila melanogaster kelch like family member 10 (klhl10), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa00005
Clone ID Related Accession (Same CDS sequence)	NM_001015100.2 , NM_001015101.2
Accession Version	NM_001015101.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kelch like family member 10, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NW_007931083). On Nov 20, 2007 this sequence version replaced NM_001015101.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTCGTA ATCAAAACGA AAGCAACCTC CTTGATATCA ATGAAGATCT GCCTTTTGCT 
CTTGCTCTGC CCCCCGTCTC AATGGATTCG TTGCAGCTGA ACGCAGACGG CAATAGCCCC 
GAGGACAAGA CGCTCGGTAT TGGCCGTAAA ACCATTTTGA ATGCGCACAC AAAATCTTTG 
GCTCCCATTG TGAGTGACGA GGCCCTGAAC ACGCTAAATG AGCTCCGCGA GCAAAATCTT 
CTCTGCGACG CCCAGATTTC TGTGGGGGAG GATGTGTTCA ACGTACATCG TGCAATAATG 
TGTTCATGTA GCTCATACTT TCGAGCTCAA TTCACAGGGT TTAACGCTGA CACTCCCGGA 
TGTGTAGATG GGTCAGATGC AAAAAAAAAC AATAATTTTA TTCATATCCC CGGCGTGAGT 
TCATGTATAA TGAACTGTGT AATACAGTAC GCTTACCTGC GTCAAACAAA CATCTCCGAG 
AGCAATGTTC ATGAGTTACT TATCTGCGCT GACTACGTTG GCATGGTAGG TTTAGTTAAG 
AAATGTAAAG ACTACCTCGG TCGCATACTC ACCCCCGAAA ACTGTGTCAG TATTATGGGA 
TTCGCACGGT TCCGATTTTT GGAGGACCTT CACCTGAAGG CCAGGAACTA CACTCTAAGG 
TACTTTACTG AAGTTGCATA TCGTAATATT GACATACTAG ATATGTCAGC TGAAGACTTT 
TACAGTATTA TTAGCGATGA TGAGTTAAAT ACCCGCGAAG AGGATCACGT ATGGAAATTG 
TGTGTTAAAT GGATCGACCG TAATCCAGAA AGCCGCAAGC GCCATGTTGC CCATCTAATG 
ACTGGGGTAC GACTGGGCCT AATGACTCCA AAGTGCTTCA TGGAAGAGGT TAAGGAACAC 
CCATACGTCT TAGAATGTGA AGCCGCTAAG CCACTGATCG TTGACACCTT TAAATTTATG 
TACGACCTGG ACTTTTTGAA TCCTCAGGCA GACGAGCTTA CCACTCCACC ACTGGCAATG 
CCAAGGCTAC CGCACGAAGT CATCTTTGCC ATAGGCGGCT GGAGCGGTGG CACCTCAAAA 
GGTTGCATCG AGACTTATGA CACGAGGGCA GATAGATGGG TAACCATTAA CGCAGAGGAT 
CCTGCTGGTC CCCGTGCGTA TCATGGAACC GCAGTGCTGG GTTTCAAAAT ATTCTCGATT 
GGCGGATATG ATGGCGTAGA GTATTTCAAC ACATGCCGAG TTTTTGACGC GGTAAAAAAA 
AAATGGAACG AAATCGCACC GATGCATTGC AGGCGCTGCT ACGTAAGTGT AACAGAGTTA 
AATGGAATGA TATATGCAAT CGGCGGCTAC GACGGGCATA ACCGGCTGAA CACTGTGGAG 
CGGTATAATC CAAGGACCAA CCAATGGTCC GTTATCCCAC CAATGAACAT GCAACGCTCT 
GATGCCAGCG CCTGTACTTT GCAAGAACGC ATATATGCTA CAGGGGGATT TAACGGCCAG 
GAATGCCTAG ACAGCGCCGA GTATTATGAC CCTGTAACCA ACGTTTGGAC TAGAATACCT 
AACATGAATC ATCGTCGGTC TGGAGTGAGC TGCGTGGCCT TTAGAAACCA GCTTTACGTT 
ATCGGCGGCT TTAACGGCAC AGCGCGTCTA TCTACCGGAG AACGCTTTGA TCCGGACACG 
CAAACTTGGC ACTTCATTCG TGAAATGAAC CACTCCCGCA GTAACTTCGG GCTAGAGATT 
ATTGACGATA TGATCTTCGC TATAGGCGGC TTTAACGGCG TCTCGACGAT CTCTCATACG 
GAATGCTATG TAGCCGAAAC AGACGAATGG ATGGAAGCCA CAGATATGAA TATTGTTCGT 
TCCGCCTTGT CTGCCAATAA CATAGCTGGG CTTCCAAACA AGCGAGACTA TATACACAAA 
GAGCGGGATC GCCTGATGGA GGAGCGGCGT CAGCGTCTTA TGGCAACCGC CATGGCTCGG 
GAGAATGTAG GACACGGCAA TGCCTCGCAG ATGTTAGTGG AACACAATGA CGGAGCCATG 
GACGACTACG AAAGTCCTGA TGAAGTAGAT GAGACTGATG AGCAAGAGCA ATCAGCACGC 
ATAAGCCGGA CTATACCACC CTCTGTAGGG CTTCCAAACC CAGCAGTTGC TGCTGAAACG 
ATACGAGATC AATCTCAATT GGTGTCCACT GCTCAACTTA ATAGAGTAAT GGAGGATCGC 
GGTATGGACA ACAATAGGAG CAATCGTGTG CAACTGCGTA ATCAACGCCA TGGCTCGCAT 
CATGAAATTC GTCGTCGTAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001015101.2
CDS	156..2459
Translation	MSRNQNESNLLDINEDLPFALALPPVSMDSLQLNADGNSPEDKTLGIGRKTILNAHTKSLAPIVSDEALNTLNELREQNLLCDAQISVGEDVFNVHRAIMCSCSSYFRAQFTGFNADTPGCVDGSDAKKNNNFIHIPGVSSCIMNCVIQYAYLRQTNISESNVHELLICADYVGMVGLVKKCKDYLGRILTPENCVSIMGFARFRFLEDLHLKARNYTLRYFTEVAYRNIDILDMSAEDFYSIISDDELNTREEDHVWKLCVKWIDRNPESRKRHVAHLMTGVRLGLMTPKCFMEEVKEHPYVLECEAAKPLIVDTFKFMYDLDFLNPQADELTTPPLAMPRLPHEVIFAIGGWSGGTSKGCIETYDTRADRWVTINAEDPAGPRAYHGTAVLGFKIFSIGGYDGVEYFNTCRVFDAVKKKWNEIAPMHCRRCYVSVTELNGMIYAIGGYDGHNRLNTVERYNPRTNQWSVIPPMNMQRSDASACTLQERIYATGGFNGQECLDSAEYYDPVTNVWTRIPNMNHRRSGVSCVAFRNQLYVIGGFNGTARLSTGERFDPDTQTWHFIREMNHSRSNFGLEIIDDMIFAIGGFNGVSTISHTECYVAETDEWMEATDMNIVRSALSANNIAGLPNKRDYIHKERDRLMEERRQRLMATAMARENVGHGNASQMLVEHNDGAMDDYESPDEVDETDEQEQSARISRTIPPSVGLPNPAVAAETIRDQSQLVSTAQLNRVMEDRGMDNNRSNRVQLRNQRHGSHHEIRRRT

Target ORF information:

RefSeq Version	NM_001015101.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kelch like family member 10 (klhl10), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001015101.2

ORF Insert Sequence:

ATGAGTCGTA ATCAAAACGA AAGCAACCTC CTTGATATCA ATGAAGATCT GCCTTTTGCT 
CTTGCTCTGC CCCCCGTCTC AATGGATTCG TTGCAGCTGA ACGCAGACGG CAATAGCCCC 
GAGGACAAGA CGCTCGGTAT TGGCCGTAAA ACCATTTTGA ATGCGCACAC AAAATCTTTG 
GCTCCCATTG TGAGTGACGA GGCCCTGAAC ACGCTAAATG AGCTCCGCGA GCAAAATCTT 
CTCTGCGACG CCCAGATTTC TGTGGGGGAG GATGTGTTCA ACGTACATCG TGCAATAATG 
TGTTCATGTA GCTCATACTT TCGAGCTCAA TTCACAGGGT TTAACGCTGA CACTCCCGGA 
TGTGTAGATG GGTCAGATGC AAAAAAAAAC AATAATTTTA TTCATATCCC CGGCGTGAGT 
TCATGTATAA TGAACTGTGT AATACAGTAC GCTTACCTGC GTCAAACAAA CATCTCCGAG 
AGCAATGTTC ATGAGTTACT TATCTGCGCT GACTACGTTG GCATGGTAGG TTTAGTTAAG 
AAATGTAAAG ACTACCTCGG TCGCATACTC ACCCCCGAAA ACTGTGTCAG TATTATGGGA 
TTCGCACGGT TCCGATTTTT GGAGGACCTT CACCTGAAGG CCAGGAACTA CACTCTAAGG 
TACTTTACTG AAGTTGCATA TCGTAATATT GACATACTAG ATATGTCAGC TGAAGACTTT 
TACAGTATTA TTAGCGATGA TGAGTTAAAT ACCCGCGAAG AGGATCACGT ATGGAAATTG 
TGTGTTAAAT GGATCGACCG TAATCCAGAA AGCCGCAAGC GCCATGTTGC CCATCTAATG 
ACTGGGGTAC GACTGGGCCT AATGACTCCA AAGTGCTTCA TGGAAGAGGT TAAGGAACAC 
CCATACGTCT TAGAATGTGA AGCCGCTAAG CCACTGATCG TTGACACCTT TAAATTTATG 
TACGACCTGG ACTTTTTGAA TCCTCAGGCA GACGAGCTTA CCACTCCACC ACTGGCAATG 
CCAAGGCTAC CGCACGAAGT CATCTTTGCC ATAGGCGGCT GGAGCGGTGG CACCTCAAAA 
GGTTGCATCG AGACTTATGA CACGAGGGCA GATAGATGGG TAACCATTAA CGCAGAGGAT 
CCTGCTGGTC CCCGTGCGTA TCATGGAACC GCAGTGCTGG GTTTCAAAAT ATTCTCGATT 
GGCGGATATG ATGGCGTAGA GTATTTCAAC ACATGCCGAG TTTTTGACGC GGTAAAAAAA 
AAATGGAACG AAATCGCACC GATGCATTGC AGGCGCTGCT ACGTAAGTGT AACAGAGTTA 
AATGGAATGA TATATGCAAT CGGCGGCTAC GACGGGCATA ACCGGCTGAA CACTGTGGAG 
CGGTATAATC CAAGGACCAA CCAATGGTCC GTTATCCCAC CAATGAACAT GCAACGCTCT 
GATGCCAGCG CCTGTACTTT GCAAGAACGC ATATATGCTA CAGGGGGATT TAACGGCCAG 
GAATGCCTAG ACAGCGCCGA GTATTATGAC CCTGTAACCA ACGTTTGGAC TAGAATACCT 
AACATGAATC ATCGTCGGTC TGGAGTGAGC TGCGTGGCCT TTAGAAACCA GCTTTACGTT 
ATCGGCGGCT TTAACGGCAC AGCGCGTCTA TCTACCGGAG AACGCTTTGA TCCGGACACG 
CAAACTTGGC ACTTCATTCG TGAAATGAAC CACTCCCGCA GTAACTTCGG GCTAGAGATT 
ATTGACGATA TGATCTTCGC TATAGGCGGC TTTAACGGCG TCTCGACGAT CTCTCATACG 
GAATGCTATG TAGCCGAAAC AGACGAATGG ATGGAAGCCA CAGATATGAA TATTGTTCGT 
TCCGCCTTGT CTGCCAATAA CATAGCTGGG CTTCCAAACA AGCGAGACTA TATACACAAA 
GAGCGGGATC GCCTGATGGA GGAGCGGCGT CAGCGTCTTA TGGCAACCGC CATGGCTCGG 
GAGAATGTAG GACACGGCAA TGCCTCGCAG ATGTTAGTGG AACACAATGA CGGAGCCATG 
GACGACTACG AAAGTCCTGA TGAAGTAGAT GAGACTGATG AGCAAGAGCA ATCAGCACGC 
ATAAGCCGGA CTATACCACC CTCTGTAGGG CTTCCAAACC CAGCAGTTGC TGCTGAAACG 
ATACGAGATC AATCTCAATT GGTGTCCACT GCTCAACTTA ATAGAGTAAT GGAGGATCGC 
GGTATGGACA ACAATAGGAG CAATCGTGTG CAACTGCGTA ATCAACGCCA TGGCTCGCAT 
CATGAAATTC GTCGTCGTAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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klhl10 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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