daf-12 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	daf-12
Entrez Gene ID	181263
Full Name	Nuclear hormone receptor family member daf-12
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001029376.4	NP_001024547.1	Nuclear hormone receptor family member daf-12
NM_001029378.1	NP_001024549.1	Nuclear hormone receptor family member daf-12
NM_001375116.1	NP_001361879.1	Nuclear hormone receptor family member daf-12
NM_001047774.2	NP_001041239.1	Nuclear hormone receptor family member daf-12
NM_001029377.3	NP_001024548.1	Nuclear hormone receptor family member daf-12
NM_001029376.5	NP_001024547.1	Nuclear hormone receptor family member daf-12
NM_001047774.4	NP_001041239.1	Nuclear hormone receptor family member daf-12
NM_001373486.1	NP_001359629.1	Nuclear hormone receptor family member daf-12

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pdda is abolished by synthetic ascarosides and steroidal ligands of the nuclear hormone receptor DAF-12. We show that neither ascarosides nor any known steroid hormones are required for Pdda and that another chemical signal mediates this phenotype, in part via the nuclear hormone receptor NHR-8.
Title: Larval crowding accelerates C. elegans development and reduces lifespan.

We consider irg-7 to be an integral downstream component of the germline longevity pathway because its expression increases upon germ cell removal and its depletion interferes with the activation of the longevity-promoting transcription factors DAF-16 and DAF-12 in germlineless animals.
Title: Innate immunity mediated longevity and longevity induced by germ cell removal converge on the C-type lectin domain protein IRG-7.

When bound to its ligand, DAF-12 acquires transcriptional activity that directs reproductive development, while unliganded DAF-12 forms a dauer-specifying complex with its interacting protein DIN-1S to regulate the transcription of genes required for dauer development
Title: Developmental and Cell Cycle Quiescence Is Mediated by the Nuclear Hormone Receptor Coregulator DIN-1S in the Caenorhabditis elegans Dauer Larva.

The dbl-1/TGF-beta and daf-12/NHR pathways have not previously been shown to affect L1 development, but disruption of these pathways delayed L1 development in fed larvae, consistent with these pathways promoting development in starved daf-16/FOXO mutants.
Title: dbl-1/TGF-? and daf-12/NHR Signaling Mediate Cell-Nonautonomous Effects of daf-16/FOXO on Starvation-Induced Developmental Arrest.

Under daf-12 RNAi rescue of survival by carnitine was abolished. RNA-interference for gamma-butyrobetaine hydroxylase 2, encoding the key enzyme for carnitine biosynthesis did neither increase glucose toxicity nor prevent the rescue of survival by carnitine
Title: Carnitine protects the nematode Caenorhabditis elegans from glucose-induced reduction of survival depending on the nuclear hormone receptor DAF-12.

The following daf-12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the daf-12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa348970	NM_001029378.1 Latest version!	Caenorhabditis elegans Nuclear hormone receptor family member daf-12 (daf-12), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OCa348970	NM_001373486.1 Latest version!	Caenorhabditis elegans Nuclear hormone receptor family member daf-12 (daf-12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OCa348968	NM_001375116.1 Latest version!	Caenorhabditis elegans Nuclear hormone receptor family member daf-12 (daf-12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OCa348968	NM_001029377.3 Latest version!	Caenorhabditis elegans Nuclear hormone receptor family member daf-12 (daf-12), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OCa348969	NM_001029376.5 Latest version!	Caenorhabditis elegans Nuclear hormone receptor family member daf-12 (daf-12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OCa348977	NM_001047774.4 Latest version!	Caenorhabditis elegans Nuclear hormone receptor family member daf-12 (daf-12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OCa348969	NM_001029376.4	Caenorhabditis elegans Nuclear hormone receptor family member daf-12 (daf-12), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OCa348977	NM_001047774.2	Caenorhabditis elegans Nuclear hormone receptor family member daf-12 (daf-12), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

daf-12 ( NM_001029378.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001024549.1 Caenorhabditis elegans Nuclear hormone receptor family member daf-12 (daf-12), partial mRNA.

Price & Availability↑

CloneID	OCa348970
Clone ID Related Accession (Same CDS sequence)	NM_001029378.1 , NM_001373486.1
Accession Version	NM_001029378.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Nuclear hormone receptor family member daf-12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on both ends.

ATGACTCCAA CACATGGTTT TCAATATCAA TCTGATCCGT ATCAAGTTCC ACCAGCAGAA 
AGAAACATCA ATTATCAACT GAATGCCGCT GAGCTTAAAG CACTGGATGC TGTCCGAGAA 
GCTTTCTATG GTATGGACGA CCCAATGGAG CAGGGACGTC AAATGCAGTC ATTCCTTAAG 
GCAAACAAAA CTCCAGCTGA TATTATGAAT ATTATGGATG TTACCATGAG GCGTTTCGTC 
AAAGTTGCCA AAGGTGTTCC GGCGTTCCGT GAGGTATCTC AGGAAGGAAA GTTTTCGCTT 
CTCAAGGGGG GAATGATTGA AATGCTGACA GTCCGTGGTG TGACCCGCTA CGACGCTTCG 
ACCAACTCTT TCAAGACACC AACAATTAAG GGGCAAAACG TTTCCGTCAA CGTGGATGAT 
ATGTTTGCTA AATTGAATGC AAATGCACAA GCTCAGAAAG CTAAATGTTT AGAGTTCTTC 
GGTTTCTTCG ACGAGGAGAT TAAAAAGAAT GAACTTGCCG TTTATCTAGT CATGTTGGCT 
GTTCTCTTCT CTGTCCGCTC TGATCCTCCG ATGAACGAAA ACGATGTTCG CATCGTTACA 
GAACGACATA ATCATTTCAT GAGTCTTCTC AACAGATATT TGGAATCGTT GTTCGGAGAG 
CAGGCCCGCC GTATCTTTGA ACGGATTCCA AAAGCACTGG GATTACTTAA TGAAATTGCT 
CGTAATGCTG GAATGCTGTT CATGGGAACT GTGCGTTCTG GTGAAGCCGA AGAGCTGCCA 
GGAGAATTTT TCAAAATCAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001024549.1
CDS	1..804
Translation	MTPTHGFQYQSDPYQVPPAERNINYQLNAAELKALDAVREAFYGMDDPMEQGRQMQSFLKANKTPADIMNIMDVTMRRFVKVAKGVPAFREVSQEGKFSLLKGGMIEMLTVRGVTRYDASTNSFKTPTIKGQNVSVNVDDMFAKLNANAQAQKAKCLEFFGFFDEEIKKNELAVYLVMLAVLFSVRSDPPMNENDVRIVTERHNHFMSLLNRYLESLFGEQARRIFERIPKALGLLNEIARNAGMLFMGTVRSGEAEELPGEFFKIK

Target ORF information:

RefSeq Version	NM_001029378.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Nuclear hormone receptor family member daf-12 (daf-12), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001029378.1

ORF Insert Sequence:

ATGACTCCAA CACATGGTTT TCAATATCAA TCTGATCCGT ATCAAGTTCC ACCAGCAGAA 
AGAAACATCA ATTATCAACT GAATGCCGCT GAGCTTAAAG CACTGGATGC TGTCCGAGAA 
GCTTTCTATG GTATGGACGA CCCAATGGAG CAGGGACGTC AAATGCAGTC ATTCCTTAAG 
GCAAACAAAA CTCCAGCTGA TATTATGAAT ATTATGGATG TTACCATGAG GCGTTTCGTC 
AAAGTTGCCA AAGGTGTTCC GGCGTTCCGT GAGGTATCTC AGGAAGGAAA GTTTTCGCTT 
CTCAAGGGGG GAATGATTGA AATGCTGACA GTCCGTGGTG TGACCCGCTA CGACGCTTCG 
ACCAACTCTT TCAAGACACC AACAATTAAG GGGCAAAACG TTTCCGTCAA CGTGGATGAT 
ATGTTTGCTA AATTGAATGC AAATGCACAA GCTCAGAAAG CTAAATGTTT AGAGTTCTTC 
GGTTTCTTCG ACGAGGAGAT TAAAAAGAAT GAACTTGCCG TTTATCTAGT CATGTTGGCT 
GTTCTCTTCT CTGTCCGCTC TGATCCTCCG ATGAACGAAA ACGATGTTCG CATCGTTACA 
GAACGACATA ATCATTTCAT GAGTCTTCTC AACAGATATT TGGAATCGTT GTTCGGAGAG 
CAGGCCCGCC GTATCTTTGA ACGGATTCCA AAAGCACTGG GATTACTTAA TGAAATTGCT 
CGTAATGCTG GAATGCTGTT CATGGGAACT GTGCGTTCTG GTGAAGCCGA AGAGCTGCCA 
GGAGAATTTT TCAAAATCAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

daf-12 ( NM_001373486.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001359629.1 Caenorhabditis elegans Nuclear hormone receptor family member daf-12 (daf-12), mRNA.

Price & Availability↑

CloneID	OCa348970
Clone ID Related Accession (Same CDS sequence)	NM_001029378.1 , NM_001373486.1
Accession Version	NM_001373486.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Nuclear hormone receptor family member daf-12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284).

ATGACTCCAA CACATGGTTT TCAATATCAA TCTGATCCGT ATCAAGTTCC ACCAGCAGAA 
AGAAACATCA ATTATCAACT GAATGCCGCT GAGCTTAAAG CACTGGATGC TGTCCGAGAA 
GCTTTCTATG GTATGGACGA CCCAATGGAG CAGGGACGTC AAATGCAGTC ATTCCTTAAG 
GCAAACAAAA CTCCAGCTGA TATTATGAAT ATTATGGATG TTACCATGAG GCGTTTCGTC 
AAAGTTGCCA AAGGTGTTCC GGCGTTCCGT GAGGTATCTC AGGAAGGAAA GTTTTCGCTT 
CTCAAGGGGG GAATGATTGA AATGCTGACA GTCCGTGGTG TGACCCGCTA CGACGCTTCG 
ACCAACTCTT TCAAGACACC AACAATTAAG GGGCAAAACG TTTCCGTCAA CGTGGATGAT 
ATGTTTGCTA AATTGAATGC AAATGCACAA GCTCAGAAAG CTAAATGTTT AGAGTTCTTC 
GGTTTCTTCG ACGAGGAGAT TAAAAAGAAT GAACTTGCCG TTTATCTAGT CATGTTGGCT 
GTTCTCTTCT CTGTCCGCTC TGATCCTCCG ATGAACGAAA ACGATGTTCG CATCGTTACA 
GAACGACATA ATCATTTCAT GAGTCTTCTC AACAGATATT TGGAATCGTT GTTCGGAGAG 
CAGGCCCGCC GTATCTTTGA ACGGATTCCA AAAGCACTGG GATTACTTAA TGAAATTGCT 
CGTAATGCTG GAATGCTGTT CATGGGAACT GTGCGTTCTG GTGAAGCCGA AGAGCTGCCA 
GGAGAATTTT TCAAAATCAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001359629.1
CDS	1442..2245
Translation	MTPTHGFQYQSDPYQVPPAERNINYQLNAAELKALDAVREAFYGMDDPMEQGRQMQSFLKANKTPADIMNIMDVTMRRFVKVAKGVPAFREVSQEGKFSLLKGGMIEMLTVRGVTRYDASTNSFKTPTIKGQNVSVNVDDMFAKLNANAQAQKAKCLEFFGFFDEEIKKNELAVYLVMLAVLFSVRSDPPMNENDVRIVTERHNHFMSLLNRYLESLFGEQARRIFERIPKALGLLNEIARNAGMLFMGTVRSGEAEELPGEFFKIK

Target ORF information:

RefSeq Version	NM_001373486.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Nuclear hormone receptor family member daf-12 (daf-12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001373486.1

ORF Insert Sequence:

ATGACTCCAA CACATGGTTT TCAATATCAA TCTGATCCGT ATCAAGTTCC ACCAGCAGAA 
AGAAACATCA ATTATCAACT GAATGCCGCT GAGCTTAAAG CACTGGATGC TGTCCGAGAA 
GCTTTCTATG GTATGGACGA CCCAATGGAG CAGGGACGTC AAATGCAGTC ATTCCTTAAG 
GCAAACAAAA CTCCAGCTGA TATTATGAAT ATTATGGATG TTACCATGAG GCGTTTCGTC 
AAAGTTGCCA AAGGTGTTCC GGCGTTCCGT GAGGTATCTC AGGAAGGAAA GTTTTCGCTT 
CTCAAGGGGG GAATGATTGA AATGCTGACA GTCCGTGGTG TGACCCGCTA CGACGCTTCG 
ACCAACTCTT TCAAGACACC AACAATTAAG GGGCAAAACG TTTCCGTCAA CGTGGATGAT 
ATGTTTGCTA AATTGAATGC AAATGCACAA GCTCAGAAAG CTAAATGTTT AGAGTTCTTC 
GGTTTCTTCG ACGAGGAGAT TAAAAAGAAT GAACTTGCCG TTTATCTAGT CATGTTGGCT 
GTTCTCTTCT CTGTCCGCTC TGATCCTCCG ATGAACGAAA ACGATGTTCG CATCGTTACA 
GAACGACATA ATCATTTCAT GAGTCTTCTC AACAGATATT TGGAATCGTT GTTCGGAGAG 
CAGGCCCGCC GTATCTTTGA ACGGATTCCA AAAGCACTGG GATTACTTAA TGAAATTGCT 
CGTAATGCTG GAATGCTGTT CATGGGAACT GTGCGTTCTG GTGAAGCCGA AGAGCTGCCA 
GGAGAATTTT TCAAAATCAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

daf-12 ( NM_001375116.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001361879.1 Caenorhabditis elegans Nuclear hormone receptor family member daf-12 (daf-12), mRNA.

Price & Availability↑

CloneID	OCa348968
Clone ID Related Accession (Same CDS sequence)	NM_001375116.1 , NM_001029377.3
Accession Version	NM_001375116.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Nuclear hormone receptor family member daf-12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284).

ATGAGCACAC CAGATGATCC AGTCATCCAC AGTCCACCAT CAATTGGAGC AGCTCCTGGT 
ATGAATGGGT ATCATGGATC AGGAGTGAAA CTTGAAGAAA GTTCTGGTGC TTGTGGCTCT 
CCGGATGATG GCCTGTTAGA CTCCAGCGAA GAGAGTCGAC GACGTCAGAA AACATGTAGA 
GTATGTGGAG ATCATGCGAC GGGCTATAAT TTCAACGTTA TCACATGCGA ATCTTGTAAA 
GCATTCTTCA GGAGAAATGC GTTACGTCCC AAGGAGTTCA AATGTCCATA TTCTGAAGAT 
TGCGAAATTA ATTCTGTAAG CAGACGGTTT TGTCAGAAAT GTCGACTGAG GAAATGCTTC 
ACAGTTGGTA TGAAAAAAGA GTGGATTTTA AACGAAGAAC AACTGCGGCG GAGGAAAAAC 
TCTCGTTTGA ACAATACGGG AACGTGTAAT AAAAGATCCC AGCCAGGGAA TCAGCAGTCA 
CCACAAGGTC CTAATCAACA GCCACATTTA TCACCGCATC ATCCAGGAGT TGCAATATAT 
CCACCACAAC CACAACGCCC ACTAACAATC AATCCAATGG ACAATCAAAT GATGCATCAT 
ATGCAAGCTA ACAGACCAAA TGCCATGCCA CAACTTATCA GTCCACCAGG AGCACAACCG 
TATCCATTGA CATCCCCAGT CGGCTCTAGT GCATCTGACT CTCCACCAAA CCGAAGTTTA 
ACAATGATGC ACAACGGCGA AAAAAGTCCA GATGGCTATG ATCCAAACAT TATGGCTCAT 
CGTGCCCCGC CGCCATCCTT CAACAATAGG CCTAAAATGG ATAGTGGACA GGTTGTTCTT 
TCCACGGAAG AATATAAACA ACTCTTGTCT CGAATTCCCG GTGCCCAAGT CCCTGGTCTT 
ATGAATGAAG AAGAACCTAT CAACAAACGT GCGGCATACA ATTGCAATGG TCATCCAATG 
CCAGCTGAAA CAACACCACC CTACAGTGCT CCGATGAGTG ACATGAGCCT CTCGCGCCAC 
AACTCCACAT CATCTGGTAC TGAAAAAAAC CATATGACGC ATTCAACCGT GTCAGCCATT 
CCAGGCAATA GTGCACAAAA TCATTTCGAT ATTGCATCGT TTGGTATGGG AATCGTGACT 
GCAACCGGAG GTGGTGATGC TGCCGAAGAG ATGTACAAAC GGATGAACAT GTTTTACGAA 
AATTGCATCC AATCGGCTCT CGACAGCCCA GAAAATCAAG AACCAAAACC ACAAGAGGCA 
ATGATTCCAA AGGAAGAATA CATGACTCCA ACACATGGTT TTCAATATCA ATCTGATCCG 
TATCAAGTTC CACCAGCAGA AAGAAACATC AATTATCAAC TGAATGCCGC TGAGCTTAAA 
GCACTGGATG CTGTCCGAGA AGCTTTCTAT GGTATGGACG ACCCAATGGA GCAGGGACGT 
CAAATGCAGT CATTCCTTAA GGCAAACAAA ACTCCAGCTG ATATTATGAA TATTATGGAT 
GTTACCATGA GGCGTTTCGT CAAAGTTGCC AAAGGTGTTC CGGCGTTCCG TGAGGTATCT 
CAGGAAGGAA AGTTTTCGCT TCTCAAGGGG GGAATGATTG AAATGCTGAC AGTCCGTGGT 
GTGACCCGCT ACGACGCTTC GACCAACTCT TTCAAGACAC CAACAATTAA GGGGCAAAAC 
GTTTCCGTCA ACGTGGATGA TATGTTTGCT AAATTGAATG CAAATGCACA AGCTCAGAAA 
GCTAAATGTT TAGAGTTCTT CGGTTTCTTC GACGAGGAGA TTAAAAAGAA TGAACTTGCC 
GTTTATCTAG TCATGTTGGC TGTTCTCTTC TCTGTCCGCT CTGATCCTCC GATGAACGAA 
AACGATGTTC GCATCGTTAC AGAACGACAT AATCATTTCA TGAGTCTTCT CAACAGATAT 
TTGGAATCGT TGTTCGGAGA GCAGGCCCGC CGTATCTTTG AACGGATTCC AAAAGCACTG 
GGATTACTTA ATGAAATTGC TCGTAATGCT GGAATGCTGT TCATGGGAAC TGTGCGTTCT 
GGTGAAGCCG AAGAGCTGCC AGGAGAATTT TTCAAAATCA AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361879.1
CDS	233..2317
Translation	MSTPDDPVIHSPPSIGAAPGMNGYHGSGVKLEESSGACGSPDDGLLDSSEESRRRQKTCRVCGDHATGYNFNVITCESCKAFFRRNALRPKEFKCPYSEDCEINSVSRRFCQKCRLRKCFTVGMKKEWILNEEQLRRRKNSRLNNTGTCNKRSQPGNQQSPQGPNQQPHLSPHHPGVAIYPPQPQRPLTINPMDNQMMHHMQANRPNAMPQLISPPGAQPYPLTSPVGSSASDSPPNRSLTMMHNGEKSPDGYDPNIMAHRAPPPSFNNRPKMDSGQVVLSTEEYKQLLSRIPGAQVPGLMNEEEPINKRAAYNCNGHPMPAETTPPYSAPMSDMSLSRHNSTSSGTEKNHMTHSTVSAIPGNSAQNHFDIASFGMGIVTATGGGDAAEEMYKRMNMFYENCIQSALDSPENQEPKPQEAMIPKEEYMTPTHGFQYQSDPYQVPPAERNINYQLNAAELKALDAVREAFYGMDDPMEQGRQMQSFLKANKTPADIMNIMDVTMRRFVKVAKGVPAFREVSQEGKFSLLKGGMIEMLTVRGVTRYDASTNSFKTPTIKGQNVSVNVDDMFAKLNANAQAQKAKCLEFFGFFDEEIKKNELAVYLVMLAVLFSVRSDPPMNENDVRIVTERHNHFMSLLNRYLESLFGEQARRIFERIPKALGLLNEIARNAGMLFMGTVRSGEAEELPGEFFKIK

Target ORF information:

RefSeq Version	NM_001375116.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Nuclear hormone receptor family member daf-12 (daf-12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001375116.1

ORF Insert Sequence:

ATGAGCACAC CAGATGATCC AGTCATCCAC AGTCCACCAT CAATTGGAGC AGCTCCTGGT 
ATGAATGGGT ATCATGGATC AGGAGTGAAA CTTGAAGAAA GTTCTGGTGC TTGTGGCTCT 
CCGGATGATG GCCTGTTAGA CTCCAGCGAA GAGAGTCGAC GACGTCAGAA AACATGTAGA 
GTATGTGGAG ATCATGCGAC GGGCTATAAT TTCAACGTTA TCACATGCGA ATCTTGTAAA 
GCATTCTTCA GGAGAAATGC GTTACGTCCC AAGGAGTTCA AATGTCCATA TTCTGAAGAT 
TGCGAAATTA ATTCTGTAAG CAGACGGTTT TGTCAGAAAT GTCGACTGAG GAAATGCTTC 
ACAGTTGGTA TGAAAAAAGA GTGGATTTTA AACGAAGAAC AACTGCGGCG GAGGAAAAAC 
TCTCGTTTGA ACAATACGGG AACGTGTAAT AAAAGATCCC AGCCAGGGAA TCAGCAGTCA 
CCACAAGGTC CTAATCAACA GCCACATTTA TCACCGCATC ATCCAGGAGT TGCAATATAT 
CCACCACAAC CACAACGCCC ACTAACAATC AATCCAATGG ACAATCAAAT GATGCATCAT 
ATGCAAGCTA ACAGACCAAA TGCCATGCCA CAACTTATCA GTCCACCAGG AGCACAACCG 
TATCCATTGA CATCCCCAGT CGGCTCTAGT GCATCTGACT CTCCACCAAA CCGAAGTTTA 
ACAATGATGC ACAACGGCGA AAAAAGTCCA GATGGCTATG ATCCAAACAT TATGGCTCAT 
CGTGCCCCGC CGCCATCCTT CAACAATAGG CCTAAAATGG ATAGTGGACA GGTTGTTCTT 
TCCACGGAAG AATATAAACA ACTCTTGTCT CGAATTCCCG GTGCCCAAGT CCCTGGTCTT 
ATGAATGAAG AAGAACCTAT CAACAAACGT GCGGCATACA ATTGCAATGG TCATCCAATG 
CCAGCTGAAA CAACACCACC CTACAGTGCT CCGATGAGTG ACATGAGCCT CTCGCGCCAC 
AACTCCACAT CATCTGGTAC TGAAAAAAAC CATATGACGC ATTCAACCGT GTCAGCCATT 
CCAGGCAATA GTGCACAAAA TCATTTCGAT ATTGCATCGT TTGGTATGGG AATCGTGACT 
GCAACCGGAG GTGGTGATGC TGCCGAAGAG ATGTACAAAC GGATGAACAT GTTTTACGAA 
AATTGCATCC AATCGGCTCT CGACAGCCCA GAAAATCAAG AACCAAAACC ACAAGAGGCA 
ATGATTCCAA AGGAAGAATA CATGACTCCA ACACATGGTT TTCAATATCA ATCTGATCCG 
TATCAAGTTC CACCAGCAGA AAGAAACATC AATTATCAAC TGAATGCCGC TGAGCTTAAA 
GCACTGGATG CTGTCCGAGA AGCTTTCTAT GGTATGGACG ACCCAATGGA GCAGGGACGT 
CAAATGCAGT CATTCCTTAA GGCAAACAAA ACTCCAGCTG ATATTATGAA TATTATGGAT 
GTTACCATGA GGCGTTTCGT CAAAGTTGCC AAAGGTGTTC CGGCGTTCCG TGAGGTATCT 
CAGGAAGGAA AGTTTTCGCT TCTCAAGGGG GGAATGATTG AAATGCTGAC AGTCCGTGGT 
GTGACCCGCT ACGACGCTTC GACCAACTCT TTCAAGACAC CAACAATTAA GGGGCAAAAC 
GTTTCCGTCA ACGTGGATGA TATGTTTGCT AAATTGAATG CAAATGCACA AGCTCAGAAA 
GCTAAATGTT TAGAGTTCTT CGGTTTCTTC GACGAGGAGA TTAAAAAGAA TGAACTTGCC 
GTTTATCTAG TCATGTTGGC TGTTCTCTTC TCTGTCCGCT CTGATCCTCC GATGAACGAA 
AACGATGTTC GCATCGTTAC AGAACGACAT AATCATTTCA TGAGTCTTCT CAACAGATAT 
TTGGAATCGT TGTTCGGAGA GCAGGCCCGC CGTATCTTTG AACGGATTCC AAAAGCACTG 
GGATTACTTA ATGAAATTGC TCGTAATGCT GGAATGCTGT TCATGGGAAC TGTGCGTTCT 
GGTGAAGCCG AAGAGCTGCC AGGAGAATTT TTCAAAATCA AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

daf-12 ( NM_001029377.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001024548.1 Caenorhabditis elegans Nuclear hormone receptor family member daf-12 (daf-12), partial mRNA.

Price & Availability↑

CloneID	OCa348968
Clone ID Related Accession (Same CDS sequence)	NM_001375116.1 , NM_001029377.3
Accession Version	NM_001029377.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Nuclear hormone receptor family member daf-12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Jun 26, 2012 this sequence version replaced NM_001029377.2. COMPLETENESS: incomplete on both ends.

ATGAGCACAC CAGATGATCC AGTCATCCAC AGTCCACCAT CAATTGGAGC AGCTCCTGGT 
ATGAATGGGT ATCATGGATC AGGAGTGAAA CTTGAAGAAA GTTCTGGTGC TTGTGGCTCT 
CCGGATGATG GCCTGTTAGA CTCCAGCGAA GAGAGTCGAC GACGTCAGAA AACATGTAGA 
GTATGTGGAG ATCATGCGAC GGGCTATAAT TTCAACGTTA TCACATGCGA ATCTTGTAAA 
GCATTCTTCA GGAGAAATGC GTTACGTCCC AAGGAGTTCA AATGTCCATA TTCTGAAGAT 
TGCGAAATTA ATTCTGTAAG CAGACGGTTT TGTCAGAAAT GTCGACTGAG GAAATGCTTC 
ACAGTTGGTA TGAAAAAAGA GTGGATTTTA AACGAAGAAC AACTGCGGCG GAGGAAAAAC 
TCTCGTTTGA ACAATACGGG AACGTGTAAT AAAAGATCCC AGCCAGGGAA TCAGCAGTCA 
CCACAAGGTC CTAATCAACA GCCACATTTA TCACCGCATC ATCCAGGAGT TGCAATATAT 
CCACCACAAC CACAACGCCC ACTAACAATC AATCCAATGG ACAATCAAAT GATGCATCAT 
ATGCAAGCTA ACAGACCAAA TGCCATGCCA CAACTTATCA GTCCACCAGG AGCACAACCG 
TATCCATTGA CATCCCCAGT CGGCTCTAGT GCATCTGACT CTCCACCAAA CCGAAGTTTA 
ACAATGATGC ACAACGGCGA AAAAAGTCCA GATGGCTATG ATCCAAACAT TATGGCTCAT 
CGTGCCCCGC CGCCATCCTT CAACAATAGG CCTAAAATGG ATAGTGGACA GGTTGTTCTT 
TCCACGGAAG AATATAAACA ACTCTTGTCT CGAATTCCCG GTGCCCAAGT CCCTGGTCTT 
ATGAATGAAG AAGAACCTAT CAACAAACGT GCGGCATACA ATTGCAATGG TCATCCAATG 
CCAGCTGAAA CAACACCACC CTACAGTGCT CCGATGAGTG ACATGAGCCT CTCGCGCCAC 
AACTCCACAT CATCTGGTAC TGAAAAAAAC CATATGACGC ATTCAACCGT GTCAGCCATT 
CCAGGCAATA GTGCACAAAA TCATTTCGAT ATTGCATCGT TTGGTATGGG AATCGTGACT 
GCAACCGGAG GTGGTGATGC TGCCGAAGAG ATGTACAAAC GGATGAACAT GTTTTACGAA 
AATTGCATCC AATCGGCTCT CGACAGCCCA GAAAATCAAG AACCAAAACC ACAAGAGGCA 
ATGATTCCAA AGGAAGAATA CATGACTCCA ACACATGGTT TTCAATATCA ATCTGATCCG 
TATCAAGTTC CACCAGCAGA AAGAAACATC AATTATCAAC TGAATGCCGC TGAGCTTAAA 
GCACTGGATG CTGTCCGAGA AGCTTTCTAT GGTATGGACG ACCCAATGGA GCAGGGACGT 
CAAATGCAGT CATTCCTTAA GGCAAACAAA ACTCCAGCTG ATATTATGAA TATTATGGAT 
GTTACCATGA GGCGTTTCGT CAAAGTTGCC AAAGGTGTTC CGGCGTTCCG TGAGGTATCT 
CAGGAAGGAA AGTTTTCGCT TCTCAAGGGG GGAATGATTG AAATGCTGAC AGTCCGTGGT 
GTGACCCGCT ACGACGCTTC GACCAACTCT TTCAAGACAC CAACAATTAA GGGGCAAAAC 
GTTTCCGTCA ACGTGGATGA TATGTTTGCT AAATTGAATG CAAATGCACA AGCTCAGAAA 
GCTAAATGTT TAGAGTTCTT CGGTTTCTTC GACGAGGAGA TTAAAAAGAA TGAACTTGCC 
GTTTATCTAG TCATGTTGGC TGTTCTCTTC TCTGTCCGCT CTGATCCTCC GATGAACGAA 
AACGATGTTC GCATCGTTAC AGAACGACAT AATCATTTCA TGAGTCTTCT CAACAGATAT 
TTGGAATCGT TGTTCGGAGA GCAGGCCCGC CGTATCTTTG AACGGATTCC AAAAGCACTG 
GGATTACTTA ATGAAATTGC TCGTAATGCT GGAATGCTGT TCATGGGAAC TGTGCGTTCT 
GGTGAAGCCG AAGAGCTGCC AGGAGAATTT TTCAAAATCA AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001024548.1
CDS	1..2085
Translation	MSTPDDPVIHSPPSIGAAPGMNGYHGSGVKLEESSGACGSPDDGLLDSSEESRRRQKTCRVCGDHATGYNFNVITCESCKAFFRRNALRPKEFKCPYSEDCEINSVSRRFCQKCRLRKCFTVGMKKEWILNEEQLRRRKNSRLNNTGTCNKRSQPGNQQSPQGPNQQPHLSPHHPGVAIYPPQPQRPLTINPMDNQMMHHMQANRPNAMPQLISPPGAQPYPLTSPVGSSASDSPPNRSLTMMHNGEKSPDGYDPNIMAHRAPPPSFNNRPKMDSGQVVLSTEEYKQLLSRIPGAQVPGLMNEEEPINKRAAYNCNGHPMPAETTPPYSAPMSDMSLSRHNSTSSGTEKNHMTHSTVSAIPGNSAQNHFDIASFGMGIVTATGGGDAAEEMYKRMNMFYENCIQSALDSPENQEPKPQEAMIPKEEYMTPTHGFQYQSDPYQVPPAERNINYQLNAAELKALDAVREAFYGMDDPMEQGRQMQSFLKANKTPADIMNIMDVTMRRFVKVAKGVPAFREVSQEGKFSLLKGGMIEMLTVRGVTRYDASTNSFKTPTIKGQNVSVNVDDMFAKLNANAQAQKAKCLEFFGFFDEEIKKNELAVYLVMLAVLFSVRSDPPMNENDVRIVTERHNHFMSLLNRYLESLFGEQARRIFERIPKALGLLNEIARNAGMLFMGTVRSGEAEELPGEFFKIK

Target ORF information:

RefSeq Version	NM_001029377.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Nuclear hormone receptor family member daf-12 (daf-12), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001029377.3

ORF Insert Sequence:

ATGAGCACAC CAGATGATCC AGTCATCCAC AGTCCACCAT CAATTGGAGC AGCTCCTGGT 
ATGAATGGGT ATCATGGATC AGGAGTGAAA CTTGAAGAAA GTTCTGGTGC TTGTGGCTCT 
CCGGATGATG GCCTGTTAGA CTCCAGCGAA GAGAGTCGAC GACGTCAGAA AACATGTAGA 
GTATGTGGAG ATCATGCGAC GGGCTATAAT TTCAACGTTA TCACATGCGA ATCTTGTAAA 
GCATTCTTCA GGAGAAATGC GTTACGTCCC AAGGAGTTCA AATGTCCATA TTCTGAAGAT 
TGCGAAATTA ATTCTGTAAG CAGACGGTTT TGTCAGAAAT GTCGACTGAG GAAATGCTTC 
ACAGTTGGTA TGAAAAAAGA GTGGATTTTA AACGAAGAAC AACTGCGGCG GAGGAAAAAC 
TCTCGTTTGA ACAATACGGG AACGTGTAAT AAAAGATCCC AGCCAGGGAA TCAGCAGTCA 
CCACAAGGTC CTAATCAACA GCCACATTTA TCACCGCATC ATCCAGGAGT TGCAATATAT 
CCACCACAAC CACAACGCCC ACTAACAATC AATCCAATGG ACAATCAAAT GATGCATCAT 
ATGCAAGCTA ACAGACCAAA TGCCATGCCA CAACTTATCA GTCCACCAGG AGCACAACCG 
TATCCATTGA CATCCCCAGT CGGCTCTAGT GCATCTGACT CTCCACCAAA CCGAAGTTTA 
ACAATGATGC ACAACGGCGA AAAAAGTCCA GATGGCTATG ATCCAAACAT TATGGCTCAT 
CGTGCCCCGC CGCCATCCTT CAACAATAGG CCTAAAATGG ATAGTGGACA GGTTGTTCTT 
TCCACGGAAG AATATAAACA ACTCTTGTCT CGAATTCCCG GTGCCCAAGT CCCTGGTCTT 
ATGAATGAAG AAGAACCTAT CAACAAACGT GCGGCATACA ATTGCAATGG TCATCCAATG 
CCAGCTGAAA CAACACCACC CTACAGTGCT CCGATGAGTG ACATGAGCCT CTCGCGCCAC 
AACTCCACAT CATCTGGTAC TGAAAAAAAC CATATGACGC ATTCAACCGT GTCAGCCATT 
CCAGGCAATA GTGCACAAAA TCATTTCGAT ATTGCATCGT TTGGTATGGG AATCGTGACT 
GCAACCGGAG GTGGTGATGC TGCCGAAGAG ATGTACAAAC GGATGAACAT GTTTTACGAA 
AATTGCATCC AATCGGCTCT CGACAGCCCA GAAAATCAAG AACCAAAACC ACAAGAGGCA 
ATGATTCCAA AGGAAGAATA CATGACTCCA ACACATGGTT TTCAATATCA ATCTGATCCG 
TATCAAGTTC CACCAGCAGA AAGAAACATC AATTATCAAC TGAATGCCGC TGAGCTTAAA 
GCACTGGATG CTGTCCGAGA AGCTTTCTAT GGTATGGACG ACCCAATGGA GCAGGGACGT 
CAAATGCAGT CATTCCTTAA GGCAAACAAA ACTCCAGCTG ATATTATGAA TATTATGGAT 
GTTACCATGA GGCGTTTCGT CAAAGTTGCC AAAGGTGTTC CGGCGTTCCG TGAGGTATCT 
CAGGAAGGAA AGTTTTCGCT TCTCAAGGGG GGAATGATTG AAATGCTGAC AGTCCGTGGT 
GTGACCCGCT ACGACGCTTC GACCAACTCT TTCAAGACAC CAACAATTAA GGGGCAAAAC 
GTTTCCGTCA ACGTGGATGA TATGTTTGCT AAATTGAATG CAAATGCACA AGCTCAGAAA 
GCTAAATGTT TAGAGTTCTT CGGTTTCTTC GACGAGGAGA TTAAAAAGAA TGAACTTGCC 
GTTTATCTAG TCATGTTGGC TGTTCTCTTC TCTGTCCGCT CTGATCCTCC GATGAACGAA 
AACGATGTTC GCATCGTTAC AGAACGACAT AATCATTTCA TGAGTCTTCT CAACAGATAT 
TTGGAATCGT TGTTCGGAGA GCAGGCCCGC CGTATCTTTG AACGGATTCC AAAAGCACTG 
GGATTACTTA ATGAAATTGC TCGTAATGCT GGAATGCTGT TCATGGGAAC TGTGCGTTCT 
GGTGAAGCCG AAGAGCTGCC AGGAGAATTT TTCAAAATCA AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

daf-12 ( NM_001029376.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001024547.1 Caenorhabditis elegans Nuclear hormone receptor family member daf-12 (daf-12), partial mRNA.

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CloneID	OCa348969
Clone ID Related Accession (Same CDS sequence)	NM_001029376.4 , NM_001029376.5
Accession Version	NM_001029376.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Nuclear hormone receptor family member daf-12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Jun 26, 2012 this sequence version replaced NM_001029376.3. COMPLETENESS: incomplete on both ends.

ATGGGCACAA ATGGAGGAGT CATTGCGGAA CAGTCAATGG AAATCGAAAC AAATGAAAAC 
CCAGATAAGG TAGAGGAGCC CGTCGTGAGA AGGAAACGAG TAACCCGCAG GCGCCACAGG 
CGGATACACA GCAAGAATAA CTGCTTGACA CCTCCAAACA GCGATGACGA CCCTCAAATG 
AGCACACCAG ATGATCCAGT CATCCACAGT CCACCATCAA TTGGAGCAGC TCCTGGTATG 
AATGGGTATC ATGGATCAGG AGTGAAACTT GAAGAAAGTT CTGGTGCTTG TGGCTCTCCG 
GATGATGGCC TGTTAGACTC CAGCGAAGAG AGTCGACGAC GTCAGAAAAC ATGTAGAGTA 
TGTGGAGATC ATGCGACGGG CTATAATTTC AACGTTATCA CATGCGAATC TTGTAAAGCA 
TTCTTCAGGA GAAATGCGTT ACGTCCCAAG GAGTTCAAAT GTCCATATTC TGAAGATTGC 
GAAATTAATT CTGTAAGCAG ACGGTTTTGT CAGAAATGTC GACTGAGGAA ATGCTTCACA 
GTTGGTATGA AAAAAGAGTG GATTTTAAAC GAAGAACAAC TGCGGCGGAG GAAAAACTCT 
CGTTTGAACA ATACGGGAAC GTGTAATAAA AGATCCCAGC CAGGGAATCA GCAGTCACCA 
CAAGGTCCTA ATCAACAGCC ACATTTATCA CCGCATCATC CAGGAGTTGC AATATATCCA 
CCACAACCAC AACGCCCACT AACAATCAAT CCAATGGACA ATCAAATGAT GCATCATATG 
CAAGCTAACA GACCAAATGC CATGCCACAA CTTATCAGTC CACCAGGAGC ACAACCGTAT 
CCATTGACAT CCCCAGTCGG CTCTAGTGCA TCTGACTCTC CACCAAACCG AAGTTTAACA 
ATGATGCACA ACGGCGAAAA AAGTCCAGAT GGCTATGATC CAAACATTAT GGCTCATCGT 
GCCCCGCCGC CATCCTTCAA CAATAGGCCT AAAATGGATA GTGGACAGGT TGTTCTTTCC 
ACGGAAGAAT ATAAACAACT CTTGTCTCGA ATTCCCGGTG CCCAAGTCCC TGGTCTTATG 
AATGAAGAAG AACCTATCAA CAAACGTGCG GCATACAATT GCAATGGTCA TCCAATGCCA 
GCTGAAACAA CACCACCCTA CAGTGCTCCG ATGAGTGACA TGAGCCTCTC GCGCCACAAC 
TCCACATCAT CTGGTACTGA AAAAAACCAT ATGACGCATT CAACCGTGTC AGCCATTCCA 
GGCAATAGTG CACAAAATCA TTTCGATATT GCATCGTTTG GTATGGGAAT CGTGACTGCA 
ACCGGAGGTG GTGATGCTGC CGAAGAGATG TACAAACGGA TGAACATGTT TTACGAAAAT 
TGCATCCAAT CGGCTCTCGA CAGCCCAGAA AATCAAGAAC CAAAACCACA AGAGGCAATG 
ATTCCAAAGG AAGAATACAT GACTCCAACA CATGGTTTTC AATATCAATC TGATCCGTAT 
CAAGTTCCAC CAGCAGAAAG AAACATCAAT TATCAACTGA ATGCCGCTGA GCTTAAAGCA 
CTGGATGCTG TCCGAGAAGC TTTCTATGGT ATGGACGACC CAATGGAGCA GGGACGTCAA 
ATGCAGTCAT TCCTTAAGGC AAACAAAACT CCAGCTGATA TTATGAATAT TATGGATGTT 
ACCATGAGGC GTTTCGTCAA AGTTGCCAAA GGTGTTCCGG CGTTCCGTGA GGTATCTCAG 
GAAGGAAAGT TTTCGCTTCT CAAGGGGGGA ATGATTGAAA TGCTGACAGT CCGTGGTGTG 
ACCCGCTACG ACGCTTCGAC CAACTCTTTC AAGACACCAA CAATTAAGGG GCAAAACGTT 
TCCGTCAACG TGGATGATAT GTTTGCTAAA TTGAATGCAA ATGCACAAGC TCAGAAAGCT 
AAATGTTTAG AGTTCTTCGG TTTCTTCGAC GAGGAGATTA AAAAGAATGA ACTTGCCGTT 
TATCTAGTCA TGTTGGCTGT TCTCTTCTCT GTCCGCTCTG ATCCTCCGAT GAACGAAAAC 
GATGTTCGCA TCGTTACAGA ACGACATAAT CATTTCATGA GTCTTCTCAA CAGATATTTG 
GAATCGTTGT TCGGAGAGCA GGCCCGCCGT ATCTTTGAAC GGATTCCAAA AGCACTGGGA 
TTACTTAATG AAATTGCTCG TAATGCTGGA ATGCTGTTCA TGGGAACTGT GCGTTCTGGT 
GAAGCCGAAG AGCTGCCAGG AGAATTTTTC AAAATCAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001024547.1
CDS	1..2262
Translation	MGTNGGVIAEQSMEIETNENPDKVEEPVVRRKRVTRRRHRRIHSKNNCLTPPNSDDDPQMSTPDDPVIHSPPSIGAAPGMNGYHGSGVKLEESSGACGSPDDGLLDSSEESRRRQKTCRVCGDHATGYNFNVITCESCKAFFRRNALRPKEFKCPYSEDCEINSVSRRFCQKCRLRKCFTVGMKKEWILNEEQLRRRKNSRLNNTGTCNKRSQPGNQQSPQGPNQQPHLSPHHPGVAIYPPQPQRPLTINPMDNQMMHHMQANRPNAMPQLISPPGAQPYPLTSPVGSSASDSPPNRSLTMMHNGEKSPDGYDPNIMAHRAPPPSFNNRPKMDSGQVVLSTEEYKQLLSRIPGAQVPGLMNEEEPINKRAAYNCNGHPMPAETTPPYSAPMSDMSLSRHNSTSSGTEKNHMTHSTVSAIPGNSAQNHFDIASFGMGIVTATGGGDAAEEMYKRMNMFYENCIQSALDSPENQEPKPQEAMIPKEEYMTPTHGFQYQSDPYQVPPAERNINYQLNAAELKALDAVREAFYGMDDPMEQGRQMQSFLKANKTPADIMNIMDVTMRRFVKVAKGVPAFREVSQEGKFSLLKGGMIEMLTVRGVTRYDASTNSFKTPTIKGQNVSVNVDDMFAKLNANAQAQKAKCLEFFGFFDEEIKKNELAVYLVMLAVLFSVRSDPPMNENDVRIVTERHNHFMSLLNRYLESLFGEQARRIFERIPKALGLLNEIARNAGMLFMGTVRSGEAEELPGEFFKIK

Target ORF information:

RefSeq Version	NM_001029376.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Nuclear hormone receptor family member daf-12 (daf-12), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001029376.4

ORF Insert Sequence:

ATGGGCACAA ATGGAGGAGT CATTGCGGAA CAGTCAATGG AAATCGAAAC AAATGAAAAC 
CCAGATAAGG TAGAGGAGCC CGTCGTGAGA AGGAAACGAG TAACCCGCAG GCGCCACAGG 
CGGATACACA GCAAGAATAA CTGCTTGACA CCTCCAAACA GCGATGACGA CCCTCAAATG 
AGCACACCAG ATGATCCAGT CATCCACAGT CCACCATCAA TTGGAGCAGC TCCTGGTATG 
AATGGGTATC ATGGATCAGG AGTGAAACTT GAAGAAAGTT CTGGTGCTTG TGGCTCTCCG 
GATGATGGCC TGTTAGACTC CAGCGAAGAG AGTCGACGAC GTCAGAAAAC ATGTAGAGTA 
TGTGGAGATC ATGCGACGGG CTATAATTTC AACGTTATCA CATGCGAATC TTGTAAAGCA 
TTCTTCAGGA GAAATGCGTT ACGTCCCAAG GAGTTCAAAT GTCCATATTC TGAAGATTGC 
GAAATTAATT CTGTAAGCAG ACGGTTTTGT CAGAAATGTC GACTGAGGAA ATGCTTCACA 
GTTGGTATGA AAAAAGAGTG GATTTTAAAC GAAGAACAAC TGCGGCGGAG GAAAAACTCT 
CGTTTGAACA ATACGGGAAC GTGTAATAAA AGATCCCAGC CAGGGAATCA GCAGTCACCA 
CAAGGTCCTA ATCAACAGCC ACATTTATCA CCGCATCATC CAGGAGTTGC AATATATCCA 
CCACAACCAC AACGCCCACT AACAATCAAT CCAATGGACA ATCAAATGAT GCATCATATG 
CAAGCTAACA GACCAAATGC CATGCCACAA CTTATCAGTC CACCAGGAGC ACAACCGTAT 
CCATTGACAT CCCCAGTCGG CTCTAGTGCA TCTGACTCTC CACCAAACCG AAGTTTAACA 
ATGATGCACA ACGGCGAAAA AAGTCCAGAT GGCTATGATC CAAACATTAT GGCTCATCGT 
GCCCCGCCGC CATCCTTCAA CAATAGGCCT AAAATGGATA GTGGACAGGT TGTTCTTTCC 
ACGGAAGAAT ATAAACAACT CTTGTCTCGA ATTCCCGGTG CCCAAGTCCC TGGTCTTATG 
AATGAAGAAG AACCTATCAA CAAACGTGCG GCATACAATT GCAATGGTCA TCCAATGCCA 
GCTGAAACAA CACCACCCTA CAGTGCTCCG ATGAGTGACA TGAGCCTCTC GCGCCACAAC 
TCCACATCAT CTGGTACTGA AAAAAACCAT ATGACGCATT CAACCGTGTC AGCCATTCCA 
GGCAATAGTG CACAAAATCA TTTCGATATT GCATCGTTTG GTATGGGAAT CGTGACTGCA 
ACCGGAGGTG GTGATGCTGC CGAAGAGATG TACAAACGGA TGAACATGTT TTACGAAAAT 
TGCATCCAAT CGGCTCTCGA CAGCCCAGAA AATCAAGAAC CAAAACCACA AGAGGCAATG 
ATTCCAAAGG AAGAATACAT GACTCCAACA CATGGTTTTC AATATCAATC TGATCCGTAT 
CAAGTTCCAC CAGCAGAAAG AAACATCAAT TATCAACTGA ATGCCGCTGA GCTTAAAGCA 
CTGGATGCTG TCCGAGAAGC TTTCTATGGT ATGGACGACC CAATGGAGCA GGGACGTCAA 
ATGCAGTCAT TCCTTAAGGC AAACAAAACT CCAGCTGATA TTATGAATAT TATGGATGTT 
ACCATGAGGC GTTTCGTCAA AGTTGCCAAA GGTGTTCCGG CGTTCCGTGA GGTATCTCAG 
GAAGGAAAGT TTTCGCTTCT CAAGGGGGGA ATGATTGAAA TGCTGACAGT CCGTGGTGTG 
ACCCGCTACG ACGCTTCGAC CAACTCTTTC AAGACACCAA CAATTAAGGG GCAAAACGTT 
TCCGTCAACG TGGATGATAT GTTTGCTAAA TTGAATGCAA ATGCACAAGC TCAGAAAGCT 
AAATGTTTAG AGTTCTTCGG TTTCTTCGAC GAGGAGATTA AAAAGAATGA ACTTGCCGTT 
TATCTAGTCA TGTTGGCTGT TCTCTTCTCT GTCCGCTCTG ATCCTCCGAT GAACGAAAAC 
GATGTTCGCA TCGTTACAGA ACGACATAAT CATTTCATGA GTCTTCTCAA CAGATATTTG 
GAATCGTTGT TCGGAGAGCA GGCCCGCCGT ATCTTTGAAC GGATTCCAAA AGCACTGGGA 
TTACTTAATG AAATTGCTCG TAATGCTGGA ATGCTGTTCA TGGGAACTGT GCGTTCTGGT 
GAAGCCGAAG AGCTGCCAGG AGAATTTTTC AAAATCAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

daf-12 ( NM_001029376.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001024547.1 Caenorhabditis elegans Nuclear hormone receptor family member daf-12 (daf-12), mRNA.

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CloneID	OCa348969
Clone ID Related Accession (Same CDS sequence)	NM_001029376.4 , NM_001029376.5
Accession Version	NM_001029376.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Nuclear hormone receptor family member daf-12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Sep 4, 2019 this sequence version replaced NM_001029376.4.

ATGGGCACAA ATGGAGGAGT CATTGCGGAA CAGTCAATGG AAATCGAAAC AAATGAAAAC 
CCAGATAAGG TAGAGGAGCC CGTCGTGAGA AGGAAACGAG TAACCCGCAG GCGCCACAGG 
CGGATACACA GCAAGAATAA CTGCTTGACA CCTCCAAACA GCGATGACGA CCCTCAAATG 
AGCACACCAG ATGATCCAGT CATCCACAGT CCACCATCAA TTGGAGCAGC TCCTGGTATG 
AATGGGTATC ATGGATCAGG AGTGAAACTT GAAGAAAGTT CTGGTGCTTG TGGCTCTCCG 
GATGATGGCC TGTTAGACTC CAGCGAAGAG AGTCGACGAC GTCAGAAAAC ATGTAGAGTA 
TGTGGAGATC ATGCGACGGG CTATAATTTC AACGTTATCA CATGCGAATC TTGTAAAGCA 
TTCTTCAGGA GAAATGCGTT ACGTCCCAAG GAGTTCAAAT GTCCATATTC TGAAGATTGC 
GAAATTAATT CTGTAAGCAG ACGGTTTTGT CAGAAATGTC GACTGAGGAA ATGCTTCACA 
GTTGGTATGA AAAAAGAGTG GATTTTAAAC GAAGAACAAC TGCGGCGGAG GAAAAACTCT 
CGTTTGAACA ATACGGGAAC GTGTAATAAA AGATCCCAGC CAGGGAATCA GCAGTCACCA 
CAAGGTCCTA ATCAACAGCC ACATTTATCA CCGCATCATC CAGGAGTTGC AATATATCCA 
CCACAACCAC AACGCCCACT AACAATCAAT CCAATGGACA ATCAAATGAT GCATCATATG 
CAAGCTAACA GACCAAATGC CATGCCACAA CTTATCAGTC CACCAGGAGC ACAACCGTAT 
CCATTGACAT CCCCAGTCGG CTCTAGTGCA TCTGACTCTC CACCAAACCG AAGTTTAACA 
ATGATGCACA ACGGCGAAAA AAGTCCAGAT GGCTATGATC CAAACATTAT GGCTCATCGT 
GCCCCGCCGC CATCCTTCAA CAATAGGCCT AAAATGGATA GTGGACAGGT TGTTCTTTCC 
ACGGAAGAAT ATAAACAACT CTTGTCTCGA ATTCCCGGTG CCCAAGTCCC TGGTCTTATG 
AATGAAGAAG AACCTATCAA CAAACGTGCG GCATACAATT GCAATGGTCA TCCAATGCCA 
GCTGAAACAA CACCACCCTA CAGTGCTCCG ATGAGTGACA TGAGCCTCTC GCGCCACAAC 
TCCACATCAT CTGGTACTGA AAAAAACCAT ATGACGCATT CAACCGTGTC AGCCATTCCA 
GGCAATAGTG CACAAAATCA TTTCGATATT GCATCGTTTG GTATGGGAAT CGTGACTGCA 
ACCGGAGGTG GTGATGCTGC CGAAGAGATG TACAAACGGA TGAACATGTT TTACGAAAAT 
TGCATCCAAT CGGCTCTCGA CAGCCCAGAA AATCAAGAAC CAAAACCACA AGAGGCAATG 
ATTCCAAAGG AAGAATACAT GACTCCAACA CATGGTTTTC AATATCAATC TGATCCGTAT 
CAAGTTCCAC CAGCAGAAAG AAACATCAAT TATCAACTGA ATGCCGCTGA GCTTAAAGCA 
CTGGATGCTG TCCGAGAAGC TTTCTATGGT ATGGACGACC CAATGGAGCA GGGACGTCAA 
ATGCAGTCAT TCCTTAAGGC AAACAAAACT CCAGCTGATA TTATGAATAT TATGGATGTT 
ACCATGAGGC GTTTCGTCAA AGTTGCCAAA GGTGTTCCGG CGTTCCGTGA GGTATCTCAG 
GAAGGAAAGT TTTCGCTTCT CAAGGGGGGA ATGATTGAAA TGCTGACAGT CCGTGGTGTG 
ACCCGCTACG ACGCTTCGAC CAACTCTTTC AAGACACCAA CAATTAAGGG GCAAAACGTT 
TCCGTCAACG TGGATGATAT GTTTGCTAAA TTGAATGCAA ATGCACAAGC TCAGAAAGCT 
AAATGTTTAG AGTTCTTCGG TTTCTTCGAC GAGGAGATTA AAAAGAATGA ACTTGCCGTT 
TATCTAGTCA TGTTGGCTGT TCTCTTCTCT GTCCGCTCTG ATCCTCCGAT GAACGAAAAC 
GATGTTCGCA TCGTTACAGA ACGACATAAT CATTTCATGA GTCTTCTCAA CAGATATTTG 
GAATCGTTGT TCGGAGAGCA GGCCCGCCGT ATCTTTGAAC GGATTCCAAA AGCACTGGGA 
TTACTTAATG AAATTGCTCG TAATGCTGGA ATGCTGTTCA TGGGAACTGT GCGTTCTGGT 
GAAGCCGAAG AGCTGCCAGG AGAATTTTTC AAAATCAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001024547.1
CDS	32..2293
Translation	MGTNGGVIAEQSMEIETNENPDKVEEPVVRRKRVTRRRHRRIHSKNNCLTPPNSDDDPQMSTPDDPVIHSPPSIGAAPGMNGYHGSGVKLEESSGACGSPDDGLLDSSEESRRRQKTCRVCGDHATGYNFNVITCESCKAFFRRNALRPKEFKCPYSEDCEINSVSRRFCQKCRLRKCFTVGMKKEWILNEEQLRRRKNSRLNNTGTCNKRSQPGNQQSPQGPNQQPHLSPHHPGVAIYPPQPQRPLTINPMDNQMMHHMQANRPNAMPQLISPPGAQPYPLTSPVGSSASDSPPNRSLTMMHNGEKSPDGYDPNIMAHRAPPPSFNNRPKMDSGQVVLSTEEYKQLLSRIPGAQVPGLMNEEEPINKRAAYNCNGHPMPAETTPPYSAPMSDMSLSRHNSTSSGTEKNHMTHSTVSAIPGNSAQNHFDIASFGMGIVTATGGGDAAEEMYKRMNMFYENCIQSALDSPENQEPKPQEAMIPKEEYMTPTHGFQYQSDPYQVPPAERNINYQLNAAELKALDAVREAFYGMDDPMEQGRQMQSFLKANKTPADIMNIMDVTMRRFVKVAKGVPAFREVSQEGKFSLLKGGMIEMLTVRGVTRYDASTNSFKTPTIKGQNVSVNVDDMFAKLNANAQAQKAKCLEFFGFFDEEIKKNELAVYLVMLAVLFSVRSDPPMNENDVRIVTERHNHFMSLLNRYLESLFGEQARRIFERIPKALGLLNEIARNAGMLFMGTVRSGEAEELPGEFFKIK

Target ORF information:

RefSeq Version	NM_001029376.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Nuclear hormone receptor family member daf-12 (daf-12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001029376.5

ORF Insert Sequence:

ATGGGCACAA ATGGAGGAGT CATTGCGGAA CAGTCAATGG AAATCGAAAC AAATGAAAAC 
CCAGATAAGG TAGAGGAGCC CGTCGTGAGA AGGAAACGAG TAACCCGCAG GCGCCACAGG 
CGGATACACA GCAAGAATAA CTGCTTGACA CCTCCAAACA GCGATGACGA CCCTCAAATG 
AGCACACCAG ATGATCCAGT CATCCACAGT CCACCATCAA TTGGAGCAGC TCCTGGTATG 
AATGGGTATC ATGGATCAGG AGTGAAACTT GAAGAAAGTT CTGGTGCTTG TGGCTCTCCG 
GATGATGGCC TGTTAGACTC CAGCGAAGAG AGTCGACGAC GTCAGAAAAC ATGTAGAGTA 
TGTGGAGATC ATGCGACGGG CTATAATTTC AACGTTATCA CATGCGAATC TTGTAAAGCA 
TTCTTCAGGA GAAATGCGTT ACGTCCCAAG GAGTTCAAAT GTCCATATTC TGAAGATTGC 
GAAATTAATT CTGTAAGCAG ACGGTTTTGT CAGAAATGTC GACTGAGGAA ATGCTTCACA 
GTTGGTATGA AAAAAGAGTG GATTTTAAAC GAAGAACAAC TGCGGCGGAG GAAAAACTCT 
CGTTTGAACA ATACGGGAAC GTGTAATAAA AGATCCCAGC CAGGGAATCA GCAGTCACCA 
CAAGGTCCTA ATCAACAGCC ACATTTATCA CCGCATCATC CAGGAGTTGC AATATATCCA 
CCACAACCAC AACGCCCACT AACAATCAAT CCAATGGACA ATCAAATGAT GCATCATATG 
CAAGCTAACA GACCAAATGC CATGCCACAA CTTATCAGTC CACCAGGAGC ACAACCGTAT 
CCATTGACAT CCCCAGTCGG CTCTAGTGCA TCTGACTCTC CACCAAACCG AAGTTTAACA 
ATGATGCACA ACGGCGAAAA AAGTCCAGAT GGCTATGATC CAAACATTAT GGCTCATCGT 
GCCCCGCCGC CATCCTTCAA CAATAGGCCT AAAATGGATA GTGGACAGGT TGTTCTTTCC 
ACGGAAGAAT ATAAACAACT CTTGTCTCGA ATTCCCGGTG CCCAAGTCCC TGGTCTTATG 
AATGAAGAAG AACCTATCAA CAAACGTGCG GCATACAATT GCAATGGTCA TCCAATGCCA 
GCTGAAACAA CACCACCCTA CAGTGCTCCG ATGAGTGACA TGAGCCTCTC GCGCCACAAC 
TCCACATCAT CTGGTACTGA AAAAAACCAT ATGACGCATT CAACCGTGTC AGCCATTCCA 
GGCAATAGTG CACAAAATCA TTTCGATATT GCATCGTTTG GTATGGGAAT CGTGACTGCA 
ACCGGAGGTG GTGATGCTGC CGAAGAGATG TACAAACGGA TGAACATGTT TTACGAAAAT 
TGCATCCAAT CGGCTCTCGA CAGCCCAGAA AATCAAGAAC CAAAACCACA AGAGGCAATG 
ATTCCAAAGG AAGAATACAT GACTCCAACA CATGGTTTTC AATATCAATC TGATCCGTAT 
CAAGTTCCAC CAGCAGAAAG AAACATCAAT TATCAACTGA ATGCCGCTGA GCTTAAAGCA 
CTGGATGCTG TCCGAGAAGC TTTCTATGGT ATGGACGACC CAATGGAGCA GGGACGTCAA 
ATGCAGTCAT TCCTTAAGGC AAACAAAACT CCAGCTGATA TTATGAATAT TATGGATGTT 
ACCATGAGGC GTTTCGTCAA AGTTGCCAAA GGTGTTCCGG CGTTCCGTGA GGTATCTCAG 
GAAGGAAAGT TTTCGCTTCT CAAGGGGGGA ATGATTGAAA TGCTGACAGT CCGTGGTGTG 
ACCCGCTACG ACGCTTCGAC CAACTCTTTC AAGACACCAA CAATTAAGGG GCAAAACGTT 
TCCGTCAACG TGGATGATAT GTTTGCTAAA TTGAATGCAA ATGCACAAGC TCAGAAAGCT 
AAATGTTTAG AGTTCTTCGG TTTCTTCGAC GAGGAGATTA AAAAGAATGA ACTTGCCGTT 
TATCTAGTCA TGTTGGCTGT TCTCTTCTCT GTCCGCTCTG ATCCTCCGAT GAACGAAAAC 
GATGTTCGCA TCGTTACAGA ACGACATAAT CATTTCATGA GTCTTCTCAA CAGATATTTG 
GAATCGTTGT TCGGAGAGCA GGCCCGCCGT ATCTTTGAAC GGATTCCAAA AGCACTGGGA 
TTACTTAATG AAATTGCTCG TAATGCTGGA ATGCTGTTCA TGGGAACTGT GCGTTCTGGT 
GAAGCCGAAG AGCTGCCAGG AGAATTTTTC AAAATCAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

daf-12 ( NM_001047774.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001041239.1 Caenorhabditis elegans Nuclear hormone receptor family member daf-12 (daf-12), partial mRNA.

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CloneID	OCa348977
Clone ID Related Accession (Same CDS sequence)	NM_001047774.2 , NM_001047774.4
Accession Version	NM_001047774.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Nuclear hormone receptor family member daf-12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Jun 26, 2012 this sequence version replaced NM_001047774.1. COMPLETENESS: incomplete on both ends.

ATGGCGGACA ATTTACTTTC GTCTCAAAAC TATATTAATT GGACAATGCT GAATAAGTTC 
TATGGCAAGC CTCAAATGAG CACACCAGAT GATCCAGTCA TCCACAGTCC ACCATCAATT 
GGAGCAGCTC CTGGTATGAA TGGGTATCAT GGATCAGGAG TGAAACTTGA AGAAAGTTCT 
GGTGCTTGTG GCTCTCCGGA TGATGGCCTG TTAGACTCCA GCGAAGAGAG TCGACGACGT 
CAGAAAACAT GTAGAGTATG TGGAGATCAT GCGACGGGCT ATAATTTCAA CGTTATCACA 
TGCGAATCTT GTAAAGCATT CTTCAGGAGA AATGCGTTAC GTCCCAAGGA GTTCAAATGT 
CCATATTCTG AAGATTGCGA AATTAATTCT GTAAGCAGAC GGTTTTGTCA GAAATGTCGA 
CTGAGGAAAT GCTTCACAGT TGGTATGAAA AAAGAGTGGA TTTTAAACGA AGAACAACTG 
CGGCGGAGGA AAAACTCTCG TTTGAACAAT ACGGGAACGT GTAATAAAAG ATCCCAGCCA 
GGGAATCAGC AGTCACCACA AGGTCCTAAT CAACAGCCAC ATTTATCACC GCATCATCCA 
GGAGTTGCAA TATATCCACC ACAACCACAA CGCCCACTAA CAATCAATCC AATGGACAAT 
CAAATGATGC ATCATATGCA AGCTAACAGA CCAAATGCCA TGCCACAACT TATCAGTCCA 
CCAGGAGCAC AACCGTATCC ATTGACATCC CCAGTCGGCT CTAGTGCATC TGACTCTCCA 
CCAAACCGAA GTTTAACAAT GATGCACAAC GGCGAAAAAA GTCCAGATGG CTATGATCCA 
AACATTATGG CTCATCGTGC CCCGCCGCCA TCCTTCAACA ATAGGCCTAA AATGGATAGT 
GGACAGGTTG TTCTTTCCAC GGAAGAATAT AAACAACTCT TGTCTCGAAT TCCCGGTGCC 
CAAGTCCCTG GTCTTATGAA TGAAGAAGAA CCTATCAACA AACGTGCGGC ATACAATTGC 
AATGGTCATC CAATGCCAGC TGAAACAACA CCACCCTACA GTGCTCCGAT GAGTGACATG 
AGCCTCTCGC GCCACAACTC CACATCATCT GGTACTGAAA AAAACCATAT GACGCATTCA 
ACCGTGTCAG CCATTCCAGG CAATAGTGCA CAAAATCATT TCGATATTGC ATCGTTTGGT 
ATGGGAATCG TGACTGCAAC CGGAGGTGGT GATGCTGCCG AAGAGATGTA CAAACGGATG 
AACATGTTTT ACGAAAATTG CATCCAATCG GCTCTCGACA GCCCAGAAAA TCAAGAACCA 
AAACCACAAG AGGCAATGAT TCCAAAGGAA GAATACATGA CTCCAACACA TGGTTTTCAA 
TATCAATCTG ATCCGTATCA AGTTCCACCA GCAGAAAGAA ACATCAATTA TCAACTGAAT 
GCCGCTGAGC TTAAAGCACT GGATGCTGTC CGAGAAGCTT TCTATGGTAT GGACGACCCA 
ATGGAGCAGG GACGTCAAAT GCAGTCATTC CTTAAGGCAA ACAAAACTCC AGCTGATATT 
ATGAATATTA TGGATGTTAC CATGAGGCGT TTCGTCAAAG TTGCCAAAGG TGTTCCGGCG 
TTCCGTGAGG TATCTCAGGA AGGAAAGTTT TCGCTTCTCA AGGGGGGAAT GATTGAAATG 
CTGACAGTCC GTGGTGTGAC CCGCTACGAC GCTTCGACCA ACTCTTTCAA GACACCAACA 
ATTAAGGGGC AAAACGTTTC CGTCAACGTG GATGATATGT TTGCTAAATT GAATGCAAAT 
GCACAAGCTC AGAAAGCTAA ATGTTTAGAG TTCTTCGGTT TCTTCGACGA GGAGATTAAA 
AAGAATGAAC TTGCCGTTTA TCTAGTCATG TTGGCTGTTC TCTTCTCTGT CCGCTCTGAT 
CCTCCGATGA ACGAAAACGA TGTTCGCATC GTTACAGAAC GACATAATCA TTTCATGAGT 
CTTCTCAACA GATATTTGGA ATCGTTGTTC GGAGAGCAGG CCCGCCGTAT CTTTGAACGG 
ATTCCAAAAG CACTGGGATT ACTTAATGAA ATTGCTCGTA ATGCTGGAAT GCTGTTCATG 
GGAACTGTGC GTTCTGGTGA AGCCGAAGAG CTGCCAGGAG AATTTTTCAA AATCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001041239.1
CDS	1..2160
Translation	MADNLLSSQNYINWTMLNKFYGKPQMSTPDDPVIHSPPSIGAAPGMNGYHGSGVKLEESSGACGSPDDGLLDSSEESRRRQKTCRVCGDHATGYNFNVITCESCKAFFRRNALRPKEFKCPYSEDCEINSVSRRFCQKCRLRKCFTVGMKKEWILNEEQLRRRKNSRLNNTGTCNKRSQPGNQQSPQGPNQQPHLSPHHPGVAIYPPQPQRPLTINPMDNQMMHHMQANRPNAMPQLISPPGAQPYPLTSPVGSSASDSPPNRSLTMMHNGEKSPDGYDPNIMAHRAPPPSFNNRPKMDSGQVVLSTEEYKQLLSRIPGAQVPGLMNEEEPINKRAAYNCNGHPMPAETTPPYSAPMSDMSLSRHNSTSSGTEKNHMTHSTVSAIPGNSAQNHFDIASFGMGIVTATGGGDAAEEMYKRMNMFYENCIQSALDSPENQEPKPQEAMIPKEEYMTPTHGFQYQSDPYQVPPAERNINYQLNAAELKALDAVREAFYGMDDPMEQGRQMQSFLKANKTPADIMNIMDVTMRRFVKVAKGVPAFREVSQEGKFSLLKGGMIEMLTVRGVTRYDASTNSFKTPTIKGQNVSVNVDDMFAKLNANAQAQKAKCLEFFGFFDEEIKKNELAVYLVMLAVLFSVRSDPPMNENDVRIVTERHNHFMSLLNRYLESLFGEQARRIFERIPKALGLLNEIARNAGMLFMGTVRSGEAEELPGEFFKIK

Target ORF information:

RefSeq Version	NM_001047774.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Nuclear hormone receptor family member daf-12 (daf-12), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001047774.2

ORF Insert Sequence:

ATGGCGGACA ATTTACTTTC GTCTCAAAAC TATATTAATT GGACAATGCT GAATAAGTTC 
TATGGCAAGC CTCAAATGAG CACACCAGAT GATCCAGTCA TCCACAGTCC ACCATCAATT 
GGAGCAGCTC CTGGTATGAA TGGGTATCAT GGATCAGGAG TGAAACTTGA AGAAAGTTCT 
GGTGCTTGTG GCTCTCCGGA TGATGGCCTG TTAGACTCCA GCGAAGAGAG TCGACGACGT 
CAGAAAACAT GTAGAGTATG TGGAGATCAT GCGACGGGCT ATAATTTCAA CGTTATCACA 
TGCGAATCTT GTAAAGCATT CTTCAGGAGA AATGCGTTAC GTCCCAAGGA GTTCAAATGT 
CCATATTCTG AAGATTGCGA AATTAATTCT GTAAGCAGAC GGTTTTGTCA GAAATGTCGA 
CTGAGGAAAT GCTTCACAGT TGGTATGAAA AAAGAGTGGA TTTTAAACGA AGAACAACTG 
CGGCGGAGGA AAAACTCTCG TTTGAACAAT ACGGGAACGT GTAATAAAAG ATCCCAGCCA 
GGGAATCAGC AGTCACCACA AGGTCCTAAT CAACAGCCAC ATTTATCACC GCATCATCCA 
GGAGTTGCAA TATATCCACC ACAACCACAA CGCCCACTAA CAATCAATCC AATGGACAAT 
CAAATGATGC ATCATATGCA AGCTAACAGA CCAAATGCCA TGCCACAACT TATCAGTCCA 
CCAGGAGCAC AACCGTATCC ATTGACATCC CCAGTCGGCT CTAGTGCATC TGACTCTCCA 
CCAAACCGAA GTTTAACAAT GATGCACAAC GGCGAAAAAA GTCCAGATGG CTATGATCCA 
AACATTATGG CTCATCGTGC CCCGCCGCCA TCCTTCAACA ATAGGCCTAA AATGGATAGT 
GGACAGGTTG TTCTTTCCAC GGAAGAATAT AAACAACTCT TGTCTCGAAT TCCCGGTGCC 
CAAGTCCCTG GTCTTATGAA TGAAGAAGAA CCTATCAACA AACGTGCGGC ATACAATTGC 
AATGGTCATC CAATGCCAGC TGAAACAACA CCACCCTACA GTGCTCCGAT GAGTGACATG 
AGCCTCTCGC GCCACAACTC CACATCATCT GGTACTGAAA AAAACCATAT GACGCATTCA 
ACCGTGTCAG CCATTCCAGG CAATAGTGCA CAAAATCATT TCGATATTGC ATCGTTTGGT 
ATGGGAATCG TGACTGCAAC CGGAGGTGGT GATGCTGCCG AAGAGATGTA CAAACGGATG 
AACATGTTTT ACGAAAATTG CATCCAATCG GCTCTCGACA GCCCAGAAAA TCAAGAACCA 
AAACCACAAG AGGCAATGAT TCCAAAGGAA GAATACATGA CTCCAACACA TGGTTTTCAA 
TATCAATCTG ATCCGTATCA AGTTCCACCA GCAGAAAGAA ACATCAATTA TCAACTGAAT 
GCCGCTGAGC TTAAAGCACT GGATGCTGTC CGAGAAGCTT TCTATGGTAT GGACGACCCA 
ATGGAGCAGG GACGTCAAAT GCAGTCATTC CTTAAGGCAA ACAAAACTCC AGCTGATATT 
ATGAATATTA TGGATGTTAC CATGAGGCGT TTCGTCAAAG TTGCCAAAGG TGTTCCGGCG 
TTCCGTGAGG TATCTCAGGA AGGAAAGTTT TCGCTTCTCA AGGGGGGAAT GATTGAAATG 
CTGACAGTCC GTGGTGTGAC CCGCTACGAC GCTTCGACCA ACTCTTTCAA GACACCAACA 
ATTAAGGGGC AAAACGTTTC CGTCAACGTG GATGATATGT TTGCTAAATT GAATGCAAAT 
GCACAAGCTC AGAAAGCTAA ATGTTTAGAG TTCTTCGGTT TCTTCGACGA GGAGATTAAA 
AAGAATGAAC TTGCCGTTTA TCTAGTCATG TTGGCTGTTC TCTTCTCTGT CCGCTCTGAT 
CCTCCGATGA ACGAAAACGA TGTTCGCATC GTTACAGAAC GACATAATCA TTTCATGAGT 
CTTCTCAACA GATATTTGGA ATCGTTGTTC GGAGAGCAGG CCCGCCGTAT CTTTGAACGG 
ATTCCAAAAG CACTGGGATT ACTTAATGAA ATTGCTCGTA ATGCTGGAAT GCTGTTCATG 
GGAACTGTGC GTTCTGGTGA AGCCGAAGAG CTGCCAGGAG AATTTTTCAA AATCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

daf-12 ( NM_001047774.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001041239.1 Caenorhabditis elegans Nuclear hormone receptor family member daf-12 (daf-12), mRNA.

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CloneID	OCa348977
Clone ID Related Accession (Same CDS sequence)	NM_001047774.2 , NM_001047774.4
Accession Version	NM_001047774.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Nuclear hormone receptor family member daf-12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Oct 18, 2019 this sequence version replaced NM_001047774.3.

ATGGCGGACA ATTTACTTTC GTCTCAAAAC TATATTAATT GGACAATGCT GAATAAGTTC 
TATGGCAAGC CTCAAATGAG CACACCAGAT GATCCAGTCA TCCACAGTCC ACCATCAATT 
GGAGCAGCTC CTGGTATGAA TGGGTATCAT GGATCAGGAG TGAAACTTGA AGAAAGTTCT 
GGTGCTTGTG GCTCTCCGGA TGATGGCCTG TTAGACTCCA GCGAAGAGAG TCGACGACGT 
CAGAAAACAT GTAGAGTATG TGGAGATCAT GCGACGGGCT ATAATTTCAA CGTTATCACA 
TGCGAATCTT GTAAAGCATT CTTCAGGAGA AATGCGTTAC GTCCCAAGGA GTTCAAATGT 
CCATATTCTG AAGATTGCGA AATTAATTCT GTAAGCAGAC GGTTTTGTCA GAAATGTCGA 
CTGAGGAAAT GCTTCACAGT TGGTATGAAA AAAGAGTGGA TTTTAAACGA AGAACAACTG 
CGGCGGAGGA AAAACTCTCG TTTGAACAAT ACGGGAACGT GTAATAAAAG ATCCCAGCCA 
GGGAATCAGC AGTCACCACA AGGTCCTAAT CAACAGCCAC ATTTATCACC GCATCATCCA 
GGAGTTGCAA TATATCCACC ACAACCACAA CGCCCACTAA CAATCAATCC AATGGACAAT 
CAAATGATGC ATCATATGCA AGCTAACAGA CCAAATGCCA TGCCACAACT TATCAGTCCA 
CCAGGAGCAC AACCGTATCC ATTGACATCC CCAGTCGGCT CTAGTGCATC TGACTCTCCA 
CCAAACCGAA GTTTAACAAT GATGCACAAC GGCGAAAAAA GTCCAGATGG CTATGATCCA 
AACATTATGG CTCATCGTGC CCCGCCGCCA TCCTTCAACA ATAGGCCTAA AATGGATAGT 
GGACAGGTTG TTCTTTCCAC GGAAGAATAT AAACAACTCT TGTCTCGAAT TCCCGGTGCC 
CAAGTCCCTG GTCTTATGAA TGAAGAAGAA CCTATCAACA AACGTGCGGC ATACAATTGC 
AATGGTCATC CAATGCCAGC TGAAACAACA CCACCCTACA GTGCTCCGAT GAGTGACATG 
AGCCTCTCGC GCCACAACTC CACATCATCT GGTACTGAAA AAAACCATAT GACGCATTCA 
ACCGTGTCAG CCATTCCAGG CAATAGTGCA CAAAATCATT TCGATATTGC ATCGTTTGGT 
ATGGGAATCG TGACTGCAAC CGGAGGTGGT GATGCTGCCG AAGAGATGTA CAAACGGATG 
AACATGTTTT ACGAAAATTG CATCCAATCG GCTCTCGACA GCCCAGAAAA TCAAGAACCA 
AAACCACAAG AGGCAATGAT TCCAAAGGAA GAATACATGA CTCCAACACA TGGTTTTCAA 
TATCAATCTG ATCCGTATCA AGTTCCACCA GCAGAAAGAA ACATCAATTA TCAACTGAAT 
GCCGCTGAGC TTAAAGCACT GGATGCTGTC CGAGAAGCTT TCTATGGTAT GGACGACCCA 
ATGGAGCAGG GACGTCAAAT GCAGTCATTC CTTAAGGCAA ACAAAACTCC AGCTGATATT 
ATGAATATTA TGGATGTTAC CATGAGGCGT TTCGTCAAAG TTGCCAAAGG TGTTCCGGCG 
TTCCGTGAGG TATCTCAGGA AGGAAAGTTT TCGCTTCTCA AGGGGGGAAT GATTGAAATG 
CTGACAGTCC GTGGTGTGAC CCGCTACGAC GCTTCGACCA ACTCTTTCAA GACACCAACA 
ATTAAGGGGC AAAACGTTTC CGTCAACGTG GATGATATGT TTGCTAAATT GAATGCAAAT 
GCACAAGCTC AGAAAGCTAA ATGTTTAGAG TTCTTCGGTT TCTTCGACGA GGAGATTAAA 
AAGAATGAAC TTGCCGTTTA TCTAGTCATG TTGGCTGTTC TCTTCTCTGT CCGCTCTGAT 
CCTCCGATGA ACGAAAACGA TGTTCGCATC GTTACAGAAC GACATAATCA TTTCATGAGT 
CTTCTCAACA GATATTTGGA ATCGTTGTTC GGAGAGCAGG CCCGCCGTAT CTTTGAACGG 
ATTCCAAAAG CACTGGGATT ACTTAATGAA ATTGCTCGTA ATGCTGGAAT GCTGTTCATG 
GGAACTGTGC GTTCTGGTGA AGCCGAAGAG CTGCCAGGAG AATTTTTCAA AATCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001041239.1
CDS	354..2513
Translation	MADNLLSSQNYINWTMLNKFYGKPQMSTPDDPVIHSPPSIGAAPGMNGYHGSGVKLEESSGACGSPDDGLLDSSEESRRRQKTCRVCGDHATGYNFNVITCESCKAFFRRNALRPKEFKCPYSEDCEINSVSRRFCQKCRLRKCFTVGMKKEWILNEEQLRRRKNSRLNNTGTCNKRSQPGNQQSPQGPNQQPHLSPHHPGVAIYPPQPQRPLTINPMDNQMMHHMQANRPNAMPQLISPPGAQPYPLTSPVGSSASDSPPNRSLTMMHNGEKSPDGYDPNIMAHRAPPPSFNNRPKMDSGQVVLSTEEYKQLLSRIPGAQVPGLMNEEEPINKRAAYNCNGHPMPAETTPPYSAPMSDMSLSRHNSTSSGTEKNHMTHSTVSAIPGNSAQNHFDIASFGMGIVTATGGGDAAEEMYKRMNMFYENCIQSALDSPENQEPKPQEAMIPKEEYMTPTHGFQYQSDPYQVPPAERNINYQLNAAELKALDAVREAFYGMDDPMEQGRQMQSFLKANKTPADIMNIMDVTMRRFVKVAKGVPAFREVSQEGKFSLLKGGMIEMLTVRGVTRYDASTNSFKTPTIKGQNVSVNVDDMFAKLNANAQAQKAKCLEFFGFFDEEIKKNELAVYLVMLAVLFSVRSDPPMNENDVRIVTERHNHFMSLLNRYLESLFGEQARRIFERIPKALGLLNEIARNAGMLFMGTVRSGEAEELPGEFFKIK

Target ORF information:

RefSeq Version	NM_001047774.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Nuclear hormone receptor family member daf-12 (daf-12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001047774.4

ORF Insert Sequence:

ATGGCGGACA ATTTACTTTC GTCTCAAAAC TATATTAATT GGACAATGCT GAATAAGTTC 
TATGGCAAGC CTCAAATGAG CACACCAGAT GATCCAGTCA TCCACAGTCC ACCATCAATT 
GGAGCAGCTC CTGGTATGAA TGGGTATCAT GGATCAGGAG TGAAACTTGA AGAAAGTTCT 
GGTGCTTGTG GCTCTCCGGA TGATGGCCTG TTAGACTCCA GCGAAGAGAG TCGACGACGT 
CAGAAAACAT GTAGAGTATG TGGAGATCAT GCGACGGGCT ATAATTTCAA CGTTATCACA 
TGCGAATCTT GTAAAGCATT CTTCAGGAGA AATGCGTTAC GTCCCAAGGA GTTCAAATGT 
CCATATTCTG AAGATTGCGA AATTAATTCT GTAAGCAGAC GGTTTTGTCA GAAATGTCGA 
CTGAGGAAAT GCTTCACAGT TGGTATGAAA AAAGAGTGGA TTTTAAACGA AGAACAACTG 
CGGCGGAGGA AAAACTCTCG TTTGAACAAT ACGGGAACGT GTAATAAAAG ATCCCAGCCA 
GGGAATCAGC AGTCACCACA AGGTCCTAAT CAACAGCCAC ATTTATCACC GCATCATCCA 
GGAGTTGCAA TATATCCACC ACAACCACAA CGCCCACTAA CAATCAATCC AATGGACAAT 
CAAATGATGC ATCATATGCA AGCTAACAGA CCAAATGCCA TGCCACAACT TATCAGTCCA 
CCAGGAGCAC AACCGTATCC ATTGACATCC CCAGTCGGCT CTAGTGCATC TGACTCTCCA 
CCAAACCGAA GTTTAACAAT GATGCACAAC GGCGAAAAAA GTCCAGATGG CTATGATCCA 
AACATTATGG CTCATCGTGC CCCGCCGCCA TCCTTCAACA ATAGGCCTAA AATGGATAGT 
GGACAGGTTG TTCTTTCCAC GGAAGAATAT AAACAACTCT TGTCTCGAAT TCCCGGTGCC 
CAAGTCCCTG GTCTTATGAA TGAAGAAGAA CCTATCAACA AACGTGCGGC ATACAATTGC 
AATGGTCATC CAATGCCAGC TGAAACAACA CCACCCTACA GTGCTCCGAT GAGTGACATG 
AGCCTCTCGC GCCACAACTC CACATCATCT GGTACTGAAA AAAACCATAT GACGCATTCA 
ACCGTGTCAG CCATTCCAGG CAATAGTGCA CAAAATCATT TCGATATTGC ATCGTTTGGT 
ATGGGAATCG TGACTGCAAC CGGAGGTGGT GATGCTGCCG AAGAGATGTA CAAACGGATG 
AACATGTTTT ACGAAAATTG CATCCAATCG GCTCTCGACA GCCCAGAAAA TCAAGAACCA 
AAACCACAAG AGGCAATGAT TCCAAAGGAA GAATACATGA CTCCAACACA TGGTTTTCAA 
TATCAATCTG ATCCGTATCA AGTTCCACCA GCAGAAAGAA ACATCAATTA TCAACTGAAT 
GCCGCTGAGC TTAAAGCACT GGATGCTGTC CGAGAAGCTT TCTATGGTAT GGACGACCCA 
ATGGAGCAGG GACGTCAAAT GCAGTCATTC CTTAAGGCAA ACAAAACTCC AGCTGATATT 
ATGAATATTA TGGATGTTAC CATGAGGCGT TTCGTCAAAG TTGCCAAAGG TGTTCCGGCG 
TTCCGTGAGG TATCTCAGGA AGGAAAGTTT TCGCTTCTCA AGGGGGGAAT GATTGAAATG 
CTGACAGTCC GTGGTGTGAC CCGCTACGAC GCTTCGACCA ACTCTTTCAA GACACCAACA 
ATTAAGGGGC AAAACGTTTC CGTCAACGTG GATGATATGT TTGCTAAATT GAATGCAAAT 
GCACAAGCTC AGAAAGCTAA ATGTTTAGAG TTCTTCGGTT TCTTCGACGA GGAGATTAAA 
AAGAATGAAC TTGCCGTTTA TCTAGTCATG TTGGCTGTTC TCTTCTCTGT CCGCTCTGAT 
CCTCCGATGA ACGAAAACGA TGTTCGCATC GTTACAGAAC GACATAATCA TTTCATGAGT 
CTTCTCAACA GATATTTGGA ATCGTTGTTC GGAGAGCAGG CCCGCCGTAT CTTTGAACGG 
ATTCCAAAAG CACTGGGATT ACTTAATGAA ATTGCTCGTA ATGCTGGAAT GCTGTTCATG 
GGAACTGTGC GTTCTGGTGA AGCCGAAGAG CTGCCAGGAG AATTTTTCAA AATCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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daf-12 cDNA ORF clone, Caenorhabditis elegans

Gene

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Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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