dgk-4 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	dgk-4
Entrez Gene ID	177667
Full Name	Diacylglycerol kinase
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_069076.2	NP_501477.2	Diacylglycerol kinase
NM_069075.3	NP_501476.2	Diacylglycerol kinase
NM_069075.4	NP_501476.2	Diacylglycerol kinase
NM_069076.3	NP_501477.2	Diacylglycerol kinase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following dgk-4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dgk-4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa343746	NM_069075.4 Latest version!	Caenorhabditis elegans Diacylglycerol kinase (dgk-4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCa343747	NM_069076.3 Latest version!	Caenorhabditis elegans Diacylglycerol kinase (dgk-4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCa343746	NM_069075.3	Caenorhabditis elegans Diacylglycerol kinase (dgk-4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCa343747	NM_069076.2	Caenorhabditis elegans Diacylglycerol kinase (dgk-4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dgk-4 ( NM_069075.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_501476.2 Caenorhabditis elegans Diacylglycerol kinase (dgk-4), partial mRNA.

Price & Availability↑

CloneID	OCa343746
Clone ID Related Accession (Same CDS sequence)	NM_069075.3 , NM_069075.4
Accession Version	NM_069075.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Diacylglycerol kinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_069075.2. COMPLETENESS: incomplete on both ends.

ATGGTGGTGA CGCGTGGATT GGACAATGAT GTCGACGACG AACGAGAAGC GTGGAAACAG 
TCGATTCTGC GTGCGAAAGA TGTCATGCCG GAAAAGGCGT CTGGACCACT CGTTGCATTA 
CTTGAAGGCA CCTCGGGTCA TCATCACTGG ACAGAGACAA ATCATCCTCG GCCAACGTTT 
TGCAATTATT GCCGCGAAAA GTTGAACGGA GTCCCATGGC ACGGCTATAC GTGTGATATC 
TGCAAAGTGA AAGCACATCG AAAATGCCGT GACAATTTGA CGGAGCAATG CAAATGGACA 
AATCAATCAA GTATTCCACA ACATTTACAA TTTATTAGCC CAGAGAATTC AATTCTACCT 
CATCAATGGA TGGAAGGAAA TCTTCCAATG CCATCAAAAT GCTCAGTTTG TGAAAAGCCA 
TGTGGTTCCG TTAGAAAACT AATCGACTAT CGATGTATTT GGTGTGGATG TTGTGTCCAT 
GATACGTGTA TTGGAAATCT GGCACGAGCA TGTAGTTTAG GTCACTCGGC ACTTTCAGTA 
ATATCACCAT TAGCAATGAA AGAAGTTACT CCAAATGGGA CAGCTGTATT ACGAGAAGAA 
GCGTACGGTG GTGATTCTCC AGGCGGCAGT CCACTAATTG TACTAATCAA CGCGAAATCG 
GGTGATAGTC AGGGTCAAAG AATTATCAAA AAATTTCGAC GGATTTTGAA TCCAATTCAA 
GTTTTCGATA TTATCGCAAC TGGTCCCGAT TTTGCACTAA CATTTTTCTC GCATCTTGAA 
TCATTTCGTG TCCTTGTATG TGGTGGAGAT GGAACTGTTG GATGGGTTTT AAGCGCTTTT 
GATAGACTGA ATTTACACTC AAAGTGTCAA TTAGCAATTC TTCCACTTGG AACTGGAAAT 
GATTTGGCTC GTGTACTTGG TTGGGGACAT GCTTTTTATG ATGATACACT ACTTCCACAA 
GTTGTTCGAA CAATGGAAAG AGCACATACA AAAATGCTAG ATCGGTGGAG TGTTCTCGCT 
ATTGAAGGCC CTCAAGCAGA TGCGGTTCGT CGGTACGAGG AGCTGGTCAT CGAAAAAGTT 
CGAACGTTTC TAGAAGCTGA ACAAACATGG GACATTATAC ATGCAGCTAA TCAACTATGT 
ACAACGATTC GGGACCTTGT TCATAGTGTA TCACATACGT ATTCTGATTT AGAAAATAAC 
AAGTTGAAGC TTGATGACGA GTCAAGACTT GGAGGAAGTG ATACAATGAC GGAAAATTGC 
AATACTCTAC TGGGAAAACT CGATCGGTTG ATGAAATCAC TGAAAGAAGA CGCGAATTGT 
TTGGAGGAGT CTGGATTTGA GCCTGGTGAT GATGATGATT GTTCAACAGG AGACGAAATG 
CTCAAAAAAC GAGATTCTGT AGTAAATCGA GCGAATAGTC TTAAAAAGGC ATTCCGAGAA 
GTCATTTCAA TGGCCGAAAA GGGTATTGAT CAACACTATC GAGATCCGAA TACGGCGTGT 
ACGAAAAGAG AGAGGTTCAG GAAGAAGAGA AGTAAAACTA CGCCTAGCGT TCTTACGATA 
TCCCATTCCA ACCTGTCTTC TTCATCAGCC TGTTCCCCAC CGTGTTCACC CGCAGGAGAA 
GATCCGCCAC AAGATGATCA AAAAGAGTTT CGTAGTGCAT CTTGTAAAGC AACATTTGAA 
ATCAGTCATT CTCAATCAAG TTCATCAGCT GTTCTAACAG CACTATTCAA TGCACAATCT 
GCACGAACCT ATGATGAATT AGAGAAACCA AGTATAGGAA CAGTACATCC ACCAACTCCT 
GGAGCAACTC GTGAGCCATC GACTGCTTAT GATGATGATG AGGAAAATGA AAATGTCGAG 
AATCGAAAAG AAATTGATCA AAAATCGAAT AGTAAAAAGT CTTCAGTATC AACACCAGAA 
GATATTACAA AAGGATCACC TGATCGGCCG AGGATCTATT CTGATACCAC ACTAAACAAA 
AATATGGAGA AAATGAAAAG TCAAAGCTTG CATCCTATTT GTTCAAGTGC TGACAAGATG 
AAGCAGAGTC ACTCGGATAG TAGCTTATAT GCAGACTACG AGCATTTGGA AGGTTCATCA 
TCTGGTTGTG GTGCCGGTGC TTCAGGAAGT ACATTATCGC CTGCTCGGGC TTCTGATTCT 
GCCACCTGGT CGCGCATTAA AGAACGTAAA CGAACTGTTG GAAGTGATTT GGGATTGTCT 
AGTAGCTCTC ATTTGAGAAG TTGTCGTAAC ATGCTCTCCG GATTCGCCGG TGGATCTCTC 
ATTGCTGAAA TTTTACTATT AAATGCACGA GTACTGTCAC AGATGTCGTC ACGTCGCAAC 
TCAGCGGCAA ATATTGCAAA TCAAATTCCA ACGTCCGACT GTCAAACACC ATTCACTCAA 
TACAAGGAAA TGTGTGTAAT GAACAACTAT TTTGGAATTG GTCTTGACGC GAAAATCGCT 
CTGGAATTCC ACAACAAACG AGAAGAGTCT GAAAAAACGC GGAGCCGATC GAAACTCTTC 
ATGTGGTACG GTATTCTCGG AGGAAAAGAG CTGATGCATC GTACCTACCG TAATCTCGAG 
CAAAGGATCA AATTGGAATG TGATGGAGTT CCGATCGATC TTCCATCTCT TCAGGGAATC 
GTAATTTTGA ATATTCCATC ATATTCGGGC GGTGCAAACT TCTGGGGTCG GAACAAGGAT 
GGTCCAGAAT TCACAGTTCA AAGTTTCGAT GATCGAATTT TGGAGGTAGT TGCACTGTTC 
GGCGTGATCC ACGTGGCAAC GAGTCGTGTC CCAAATGCAG TTCGTCTCCA AAACCATCGA 
ATTGCTCAAT GTCGACATGT TCGAATTGTG ATACTCGGAG ACGAACCAAT CCCAGTTCAA 
GTTGACGGTG AACCTTGGCT TCAACCTCCT GGAATTATGC AAATTGTTCA CAAAAATCGA 
GCTCAGATGC TTGCCAGAAA TCCAGTTTTT GATGCAACAC TTAAGAAATG GGAAGAACAA 
AAAGAGAAGG CTTCCACGAC AACAGCACCA TCCACACCTA CGGCTCTTGG AGCTCCCACT 
TCAGATATTA TTTCGTTCTT GACGAGAGCT CGCGAATTTT TGAGATTGAT TGAAGGTGAA 
ATAGCGAGAT TAGGGGTTTC ATCGGTACTT TTGCAATCCT TGGATGATGC AAATGCTATT 
GTCAAAGGTG GAGCATCAGG AGAAGAAGAT CCAGAGGCTA TCGATGACTT AGAAGAACGA 
GAAGAAACAG TTGCATCGGT GGAAAGAGTC GTTGAACTTC TTGAGGATCA TTTTGGTTTT 
CCTGAAAGAA CAAGAGCACC AGTTGGGCAA CCAAAACTAG GACCCACTCC GCATTTTAAT 
TTTGAAATTC ATGGTGACGA TCCTGATCAT TGGCGTTATA TAATCAACTC GATTCGTCAG 
GATATGGATC GAGAAGAAGC AGCAATTAGA GAAGCTAGAA AACAAGATTT AATAGCTGGA 
AAGCCAAAAA GATCCCGATT CACTGCATGG TTTCACAAAC GATTTGGAAC TAGATCTGCA 
CCATTTGCAT TCGCTAATAT TCCATACTGG ACGAGCGAAG AAGTATGTGC ATGGCTCTCT 
TCAATTGGAA TGTCTGAATA TGGATCAACG TTCCGAAAGA ATGATATTCA AGGTTCTGAA 
TTGATGCATT TAGAACGATC CGATATTATG GATATTGGGA TCACAAAAAT TGGACATGTG 
AAGAGGTTGC AGTCCGCCAT TGTCGACCTC CGAGCTCAAA ACCAACGTGC ACGTCGCGCT 
CAGGCTCGTA AAAAACGTGT CGCCAAAGAC TATAAACCCG ATCATTCTGG TCCTTCTGGT 
GCTGGCGGTG CAGTAGATAA ACGAAAAGAA TCAAGTGCTG AAAGATATGC AGATGGACAA 
CCATCAACAT CATCTACGGG AGCTACCACT TCATCTGGAA AAACACGGAG AATTCCACCG 
AAAGATGGAG AATCCGCGCC TGCTGTCATG CAAATGGATC CAGCTAGTAC AGATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_501476.2
CDS	1..4020
Translation	MVVTRGLDNDVDDEREAWKQSILRAKDVMPEKASGPLVALLEGTSGHHHWTETNHPRPTFCNYCREKLNGVPWHGYTCDICKVKAHRKCRDNLTEQCKWTNQSSIPQHLQFISPENSILPHQWMEGNLPMPSKCSVCEKPCGSVRKLIDYRCIWCGCCVHDTCIGNLARACSLGHSALSVISPLAMKEVTPNGTAVLREEAYGGDSPGGSPLIVLINAKSGDSQGQRIIKKFRRILNPIQVFDIIATGPDFALTFFSHLESFRVLVCGGDGTVGWVLSAFDRLNLHSKCQLAILPLGTGNDLARVLGWGHAFYDDTLLPQVVRTMERAHTKMLDRWSVLAIEGPQADAVRRYEELVIEKVRTFLEAEQTWDIIHAANQLCTTIRDLVHSVSHTYSDLENNKLKLDDESRLGGSDTMTENCNTLLGKLDRLMKSLKEDANCLEESGFEPGDDDDCSTGDEMLKKRDSVVNRANSLKKAFREVISMAEKGIDQHYRDPNTACTKRERFRKKRSKTTPSVLTISHSNLSSSSACSPPCSPAGEDPPQDDQKEFRSASCKATFEISHSQSSSSAVLTALFNAQSARTYDELEKPSIGTVHPPTPGATREPSTAYDDDEENENVENRKEIDQKSNSKKSSVSTPEDITKGSPDRPRIYSDTTLNKNMEKMKSQSLHPICSSADKMKQSHSDSSLYADYEHLEGSSSGCGAGASGSTLSPARASDSATWSRIKERKRTVGSDLGLSSSSHLRSCRNMLSGFAGGSLIAEILLLNARVLSQMSSRRNSAANIANQIPTSDCQTPFTQYKEMCVMNNYFGIGLDAKIALEFHNKREESEKTRSRSKLFMWYGILGGKELMHRTYRNLEQRIKLECDGVPIDLPSLQGIVILNIPSYSGGANFWGRNKDGPEFTVQSFDDRILEVVALFGVIHVATSRVPNAVRLQNHRIAQCRHVRIVILGDEPIPVQVDGEPWLQPPGIMQIVHKNRAQMLARNPVFDATLKKWEEQKEKASTTTAPSTPTALGAPTSDIISFLTRAREFLRLIEGEIARLGVSSVLLQSLDDANAIVKGGASGEEDPEAIDDLEEREETVASVERVVELLEDHFGFPERTRAPVGQPKLGPTPHFNFEIHGDDPDHWRYIINSIRQDMDREEAAIREARKQDLIAGKPKRSRFTAWFHKRFGTRSAPFAFANIPYWTSEEVCAWLSSIGMSEYGSTFRKNDIQGSELMHLERSDIMDIGITKIGHVKRLQSAIVDLRAQNQRARRAQARKKRVAKDYKPDHSGPSGAGGAVDKRKESSAERYADGQPSTSSTGATTSSGKTRRIPPKDGESAPAVMQMDPASTDC

Target ORF information:

RefSeq Version	NM_069075.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Diacylglycerol kinase (dgk-4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_069075.3

ORF Insert Sequence:

ATGGTGGTGA CGCGTGGATT GGACAATGAT GTCGACGACG AACGAGAAGC GTGGAAACAG 
TCGATTCTGC GTGCGAAAGA TGTCATGCCG GAAAAGGCGT CTGGACCACT CGTTGCATTA 
CTTGAAGGCA CCTCGGGTCA TCATCACTGG ACAGAGACAA ATCATCCTCG GCCAACGTTT 
TGCAATTATT GCCGCGAAAA GTTGAACGGA GTCCCATGGC ACGGCTATAC GTGTGATATC 
TGCAAAGTGA AAGCACATCG AAAATGCCGT GACAATTTGA CGGAGCAATG CAAATGGACA 
AATCAATCAA GTATTCCACA ACATTTACAA TTTATTAGCC CAGAGAATTC AATTCTACCT 
CATCAATGGA TGGAAGGAAA TCTTCCAATG CCATCAAAAT GCTCAGTTTG TGAAAAGCCA 
TGTGGTTCCG TTAGAAAACT AATCGACTAT CGATGTATTT GGTGTGGATG TTGTGTCCAT 
GATACGTGTA TTGGAAATCT GGCACGAGCA TGTAGTTTAG GTCACTCGGC ACTTTCAGTA 
ATATCACCAT TAGCAATGAA AGAAGTTACT CCAAATGGGA CAGCTGTATT ACGAGAAGAA 
GCGTACGGTG GTGATTCTCC AGGCGGCAGT CCACTAATTG TACTAATCAA CGCGAAATCG 
GGTGATAGTC AGGGTCAAAG AATTATCAAA AAATTTCGAC GGATTTTGAA TCCAATTCAA 
GTTTTCGATA TTATCGCAAC TGGTCCCGAT TTTGCACTAA CATTTTTCTC GCATCTTGAA 
TCATTTCGTG TCCTTGTATG TGGTGGAGAT GGAACTGTTG GATGGGTTTT AAGCGCTTTT 
GATAGACTGA ATTTACACTC AAAGTGTCAA TTAGCAATTC TTCCACTTGG AACTGGAAAT 
GATTTGGCTC GTGTACTTGG TTGGGGACAT GCTTTTTATG ATGATACACT ACTTCCACAA 
GTTGTTCGAA CAATGGAAAG AGCACATACA AAAATGCTAG ATCGGTGGAG TGTTCTCGCT 
ATTGAAGGCC CTCAAGCAGA TGCGGTTCGT CGGTACGAGG AGCTGGTCAT CGAAAAAGTT 
CGAACGTTTC TAGAAGCTGA ACAAACATGG GACATTATAC ATGCAGCTAA TCAACTATGT 
ACAACGATTC GGGACCTTGT TCATAGTGTA TCACATACGT ATTCTGATTT AGAAAATAAC 
AAGTTGAAGC TTGATGACGA GTCAAGACTT GGAGGAAGTG ATACAATGAC GGAAAATTGC 
AATACTCTAC TGGGAAAACT CGATCGGTTG ATGAAATCAC TGAAAGAAGA CGCGAATTGT 
TTGGAGGAGT CTGGATTTGA GCCTGGTGAT GATGATGATT GTTCAACAGG AGACGAAATG 
CTCAAAAAAC GAGATTCTGT AGTAAATCGA GCGAATAGTC TTAAAAAGGC ATTCCGAGAA 
GTCATTTCAA TGGCCGAAAA GGGTATTGAT CAACACTATC GAGATCCGAA TACGGCGTGT 
ACGAAAAGAG AGAGGTTCAG GAAGAAGAGA AGTAAAACTA CGCCTAGCGT TCTTACGATA 
TCCCATTCCA ACCTGTCTTC TTCATCAGCC TGTTCCCCAC CGTGTTCACC CGCAGGAGAA 
GATCCGCCAC AAGATGATCA AAAAGAGTTT CGTAGTGCAT CTTGTAAAGC AACATTTGAA 
ATCAGTCATT CTCAATCAAG TTCATCAGCT GTTCTAACAG CACTATTCAA TGCACAATCT 
GCACGAACCT ATGATGAATT AGAGAAACCA AGTATAGGAA CAGTACATCC ACCAACTCCT 
GGAGCAACTC GTGAGCCATC GACTGCTTAT GATGATGATG AGGAAAATGA AAATGTCGAG 
AATCGAAAAG AAATTGATCA AAAATCGAAT AGTAAAAAGT CTTCAGTATC AACACCAGAA 
GATATTACAA AAGGATCACC TGATCGGCCG AGGATCTATT CTGATACCAC ACTAAACAAA 
AATATGGAGA AAATGAAAAG TCAAAGCTTG CATCCTATTT GTTCAAGTGC TGACAAGATG 
AAGCAGAGTC ACTCGGATAG TAGCTTATAT GCAGACTACG AGCATTTGGA AGGTTCATCA 
TCTGGTTGTG GTGCCGGTGC TTCAGGAAGT ACATTATCGC CTGCTCGGGC TTCTGATTCT 
GCCACCTGGT CGCGCATTAA AGAACGTAAA CGAACTGTTG GAAGTGATTT GGGATTGTCT 
AGTAGCTCTC ATTTGAGAAG TTGTCGTAAC ATGCTCTCCG GATTCGCCGG TGGATCTCTC 
ATTGCTGAAA TTTTACTATT AAATGCACGA GTACTGTCAC AGATGTCGTC ACGTCGCAAC 
TCAGCGGCAA ATATTGCAAA TCAAATTCCA ACGTCCGACT GTCAAACACC ATTCACTCAA 
TACAAGGAAA TGTGTGTAAT GAACAACTAT TTTGGAATTG GTCTTGACGC GAAAATCGCT 
CTGGAATTCC ACAACAAACG AGAAGAGTCT GAAAAAACGC GGAGCCGATC GAAACTCTTC 
ATGTGGTACG GTATTCTCGG AGGAAAAGAG CTGATGCATC GTACCTACCG TAATCTCGAG 
CAAAGGATCA AATTGGAATG TGATGGAGTT CCGATCGATC TTCCATCTCT TCAGGGAATC 
GTAATTTTGA ATATTCCATC ATATTCGGGC GGTGCAAACT TCTGGGGTCG GAACAAGGAT 
GGTCCAGAAT TCACAGTTCA AAGTTTCGAT GATCGAATTT TGGAGGTAGT TGCACTGTTC 
GGCGTGATCC ACGTGGCAAC GAGTCGTGTC CCAAATGCAG TTCGTCTCCA AAACCATCGA 
ATTGCTCAAT GTCGACATGT TCGAATTGTG ATACTCGGAG ACGAACCAAT CCCAGTTCAA 
GTTGACGGTG AACCTTGGCT TCAACCTCCT GGAATTATGC AAATTGTTCA CAAAAATCGA 
GCTCAGATGC TTGCCAGAAA TCCAGTTTTT GATGCAACAC TTAAGAAATG GGAAGAACAA 
AAAGAGAAGG CTTCCACGAC AACAGCACCA TCCACACCTA CGGCTCTTGG AGCTCCCACT 
TCAGATATTA TTTCGTTCTT GACGAGAGCT CGCGAATTTT TGAGATTGAT TGAAGGTGAA 
ATAGCGAGAT TAGGGGTTTC ATCGGTACTT TTGCAATCCT TGGATGATGC AAATGCTATT 
GTCAAAGGTG GAGCATCAGG AGAAGAAGAT CCAGAGGCTA TCGATGACTT AGAAGAACGA 
GAAGAAACAG TTGCATCGGT GGAAAGAGTC GTTGAACTTC TTGAGGATCA TTTTGGTTTT 
CCTGAAAGAA CAAGAGCACC AGTTGGGCAA CCAAAACTAG GACCCACTCC GCATTTTAAT 
TTTGAAATTC ATGGTGACGA TCCTGATCAT TGGCGTTATA TAATCAACTC GATTCGTCAG 
GATATGGATC GAGAAGAAGC AGCAATTAGA GAAGCTAGAA AACAAGATTT AATAGCTGGA 
AAGCCAAAAA GATCCCGATT CACTGCATGG TTTCACAAAC GATTTGGAAC TAGATCTGCA 
CCATTTGCAT TCGCTAATAT TCCATACTGG ACGAGCGAAG AAGTATGTGC ATGGCTCTCT 
TCAATTGGAA TGTCTGAATA TGGATCAACG TTCCGAAAGA ATGATATTCA AGGTTCTGAA 
TTGATGCATT TAGAACGATC CGATATTATG GATATTGGGA TCACAAAAAT TGGACATGTG 
AAGAGGTTGC AGTCCGCCAT TGTCGACCTC CGAGCTCAAA ACCAACGTGC ACGTCGCGCT 
CAGGCTCGTA AAAAACGTGT CGCCAAAGAC TATAAACCCG ATCATTCTGG TCCTTCTGGT 
GCTGGCGGTG CAGTAGATAA ACGAAAAGAA TCAAGTGCTG AAAGATATGC AGATGGACAA 
CCATCAACAT CATCTACGGG AGCTACCACT TCATCTGGAA AAACACGGAG AATTCCACCG 
AAAGATGGAG AATCCGCGCC TGCTGTCATG CAAATGGATC CAGCTAGTAC AGATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgk-4 ( NM_069075.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_501476.2 Caenorhabditis elegans Diacylglycerol kinase (dgk-4), mRNA.

Price & Availability↑

CloneID	OCa343746
Clone ID Related Accession (Same CDS sequence)	NM_069075.3 , NM_069075.4
Accession Version	NM_069075.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Diacylglycerol kinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_069075.3.

ATGGTGGTGA CGCGTGGATT GGACAATGAT GTCGACGACG AACGAGAAGC GTGGAAACAG 
TCGATTCTGC GTGCGAAAGA TGTCATGCCG GAAAAGGCGT CTGGACCACT CGTTGCATTA 
CTTGAAGGCA CCTCGGGTCA TCATCACTGG ACAGAGACAA ATCATCCTCG GCCAACGTTT 
TGCAATTATT GCCGCGAAAA GTTGAACGGA GTCCCATGGC ACGGCTATAC GTGTGATATC 
TGCAAAGTGA AAGCACATCG AAAATGCCGT GACAATTTGA CGGAGCAATG CAAATGGACA 
AATCAATCAA GTATTCCACA ACATTTACAA TTTATTAGCC CAGAGAATTC AATTCTACCT 
CATCAATGGA TGGAAGGAAA TCTTCCAATG CCATCAAAAT GCTCAGTTTG TGAAAAGCCA 
TGTGGTTCCG TTAGAAAACT AATCGACTAT CGATGTATTT GGTGTGGATG TTGTGTCCAT 
GATACGTGTA TTGGAAATCT GGCACGAGCA TGTAGTTTAG GTCACTCGGC ACTTTCAGTA 
ATATCACCAT TAGCAATGAA AGAAGTTACT CCAAATGGGA CAGCTGTATT ACGAGAAGAA 
GCGTACGGTG GTGATTCTCC AGGCGGCAGT CCACTAATTG TACTAATCAA CGCGAAATCG 
GGTGATAGTC AGGGTCAAAG AATTATCAAA AAATTTCGAC GGATTTTGAA TCCAATTCAA 
GTTTTCGATA TTATCGCAAC TGGTCCCGAT TTTGCACTAA CATTTTTCTC GCATCTTGAA 
TCATTTCGTG TCCTTGTATG TGGTGGAGAT GGAACTGTTG GATGGGTTTT AAGCGCTTTT 
GATAGACTGA ATTTACACTC AAAGTGTCAA TTAGCAATTC TTCCACTTGG AACTGGAAAT 
GATTTGGCTC GTGTACTTGG TTGGGGACAT GCTTTTTATG ATGATACACT ACTTCCACAA 
GTTGTTCGAA CAATGGAAAG AGCACATACA AAAATGCTAG ATCGGTGGAG TGTTCTCGCT 
ATTGAAGGCC CTCAAGCAGA TGCGGTTCGT CGGTACGAGG AGCTGGTCAT CGAAAAAGTT 
CGAACGTTTC TAGAAGCTGA ACAAACATGG GACATTATAC ATGCAGCTAA TCAACTATGT 
ACAACGATTC GGGACCTTGT TCATAGTGTA TCACATACGT ATTCTGATTT AGAAAATAAC 
AAGTTGAAGC TTGATGACGA GTCAAGACTT GGAGGAAGTG ATACAATGAC GGAAAATTGC 
AATACTCTAC TGGGAAAACT CGATCGGTTG ATGAAATCAC TGAAAGAAGA CGCGAATTGT 
TTGGAGGAGT CTGGATTTGA GCCTGGTGAT GATGATGATT GTTCAACAGG AGACGAAATG 
CTCAAAAAAC GAGATTCTGT AGTAAATCGA GCGAATAGTC TTAAAAAGGC ATTCCGAGAA 
GTCATTTCAA TGGCCGAAAA GGGTATTGAT CAACACTATC GAGATCCGAA TACGGCGTGT 
ACGAAAAGAG AGAGGTTCAG GAAGAAGAGA AGTAAAACTA CGCCTAGCGT TCTTACGATA 
TCCCATTCCA ACCTGTCTTC TTCATCAGCC TGTTCCCCAC CGTGTTCACC CGCAGGAGAA 
GATCCGCCAC AAGATGATCA AAAAGAGTTT CGTAGTGCAT CTTGTAAAGC AACATTTGAA 
ATCAGTCATT CTCAATCAAG TTCATCAGCT GTTCTAACAG CACTATTCAA TGCACAATCT 
GCACGAACCT ATGATGAATT AGAGAAACCA AGTATAGGAA CAGTACATCC ACCAACTCCT 
GGAGCAACTC GTGAGCCATC GACTGCTTAT GATGATGATG AGGAAAATGA AAATGTCGAG 
AATCGAAAAG AAATTGATCA AAAATCGAAT AGTAAAAAGT CTTCAGTATC AACACCAGAA 
GATATTACAA AAGGATCACC TGATCGGCCG AGGATCTATT CTGATACCAC ACTAAACAAA 
AATATGGAGA AAATGAAAAG TCAAAGCTTG CATCCTATTT GTTCAAGTGC TGACAAGATG 
AAGCAGAGTC ACTCGGATAG TAGCTTATAT GCAGACTACG AGCATTTGGA AGGTTCATCA 
TCTGGTTGTG GTGCCGGTGC TTCAGGAAGT ACATTATCGC CTGCTCGGGC TTCTGATTCT 
GCCACCTGGT CGCGCATTAA AGAACGTAAA CGAACTGTTG GAAGTGATTT GGGATTGTCT 
AGTAGCTCTC ATTTGAGAAG TTGTCGTAAC ATGCTCTCCG GATTCGCCGG TGGATCTCTC 
ATTGCTGAAA TTTTACTATT AAATGCACGA GTACTGTCAC AGATGTCGTC ACGTCGCAAC 
TCAGCGGCAA ATATTGCAAA TCAAATTCCA ACGTCCGACT GTCAAACACC ATTCACTCAA 
TACAAGGAAA TGTGTGTAAT GAACAACTAT TTTGGAATTG GTCTTGACGC GAAAATCGCT 
CTGGAATTCC ACAACAAACG AGAAGAGTCT GAAAAAACGC GGAGCCGATC GAAACTCTTC 
ATGTGGTACG GTATTCTCGG AGGAAAAGAG CTGATGCATC GTACCTACCG TAATCTCGAG 
CAAAGGATCA AATTGGAATG TGATGGAGTT CCGATCGATC TTCCATCTCT TCAGGGAATC 
GTAATTTTGA ATATTCCATC ATATTCGGGC GGTGCAAACT TCTGGGGTCG GAACAAGGAT 
GGTCCAGAAT TCACAGTTCA AAGTTTCGAT GATCGAATTT TGGAGGTAGT TGCACTGTTC 
GGCGTGATCC ACGTGGCAAC GAGTCGTGTC CCAAATGCAG TTCGTCTCCA AAACCATCGA 
ATTGCTCAAT GTCGACATGT TCGAATTGTG ATACTCGGAG ACGAACCAAT CCCAGTTCAA 
GTTGACGGTG AACCTTGGCT TCAACCTCCT GGAATTATGC AAATTGTTCA CAAAAATCGA 
GCTCAGATGC TTGCCAGAAA TCCAGTTTTT GATGCAACAC TTAAGAAATG GGAAGAACAA 
AAAGAGAAGG CTTCCACGAC AACAGCACCA TCCACACCTA CGGCTCTTGG AGCTCCCACT 
TCAGATATTA TTTCGTTCTT GACGAGAGCT CGCGAATTTT TGAGATTGAT TGAAGGTGAA 
ATAGCGAGAT TAGGGGTTTC ATCGGTACTT TTGCAATCCT TGGATGATGC AAATGCTATT 
GTCAAAGGTG GAGCATCAGG AGAAGAAGAT CCAGAGGCTA TCGATGACTT AGAAGAACGA 
GAAGAAACAG TTGCATCGGT GGAAAGAGTC GTTGAACTTC TTGAGGATCA TTTTGGTTTT 
CCTGAAAGAA CAAGAGCACC AGTTGGGCAA CCAAAACTAG GACCCACTCC GCATTTTAAT 
TTTGAAATTC ATGGTGACGA TCCTGATCAT TGGCGTTATA TAATCAACTC GATTCGTCAG 
GATATGGATC GAGAAGAAGC AGCAATTAGA GAAGCTAGAA AACAAGATTT AATAGCTGGA 
AAGCCAAAAA GATCCCGATT CACTGCATGG TTTCACAAAC GATTTGGAAC TAGATCTGCA 
CCATTTGCAT TCGCTAATAT TCCATACTGG ACGAGCGAAG AAGTATGTGC ATGGCTCTCT 
TCAATTGGAA TGTCTGAATA TGGATCAACG TTCCGAAAGA ATGATATTCA AGGTTCTGAA 
TTGATGCATT TAGAACGATC CGATATTATG GATATTGGGA TCACAAAAAT TGGACATGTG 
AAGAGGTTGC AGTCCGCCAT TGTCGACCTC CGAGCTCAAA ACCAACGTGC ACGTCGCGCT 
CAGGCTCGTA AAAAACGTGT CGCCAAAGAC TATAAACCCG ATCATTCTGG TCCTTCTGGT 
GCTGGCGGTG CAGTAGATAA ACGAAAAGAA TCAAGTGCTG AAAGATATGC AGATGGACAA 
CCATCAACAT CATCTACGGG AGCTACCACT TCATCTGGAA AAACACGGAG AATTCCACCG 
AAAGATGGAG AATCCGCGCC TGCTGTCATG CAAATGGATC CAGCTAGTAC AGATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_501476.2
CDS	94..4113
Translation	MVVTRGLDNDVDDEREAWKQSILRAKDVMPEKASGPLVALLEGTSGHHHWTETNHPRPTFCNYCREKLNGVPWHGYTCDICKVKAHRKCRDNLTEQCKWTNQSSIPQHLQFISPENSILPHQWMEGNLPMPSKCSVCEKPCGSVRKLIDYRCIWCGCCVHDTCIGNLARACSLGHSALSVISPLAMKEVTPNGTAVLREEAYGGDSPGGSPLIVLINAKSGDSQGQRIIKKFRRILNPIQVFDIIATGPDFALTFFSHLESFRVLVCGGDGTVGWVLSAFDRLNLHSKCQLAILPLGTGNDLARVLGWGHAFYDDTLLPQVVRTMERAHTKMLDRWSVLAIEGPQADAVRRYEELVIEKVRTFLEAEQTWDIIHAANQLCTTIRDLVHSVSHTYSDLENNKLKLDDESRLGGSDTMTENCNTLLGKLDRLMKSLKEDANCLEESGFEPGDDDDCSTGDEMLKKRDSVVNRANSLKKAFREVISMAEKGIDQHYRDPNTACTKRERFRKKRSKTTPSVLTISHSNLSSSSACSPPCSPAGEDPPQDDQKEFRSASCKATFEISHSQSSSSAVLTALFNAQSARTYDELEKPSIGTVHPPTPGATREPSTAYDDDEENENVENRKEIDQKSNSKKSSVSTPEDITKGSPDRPRIYSDTTLNKNMEKMKSQSLHPICSSADKMKQSHSDSSLYADYEHLEGSSSGCGAGASGSTLSPARASDSATWSRIKERKRTVGSDLGLSSSSHLRSCRNMLSGFAGGSLIAEILLLNARVLSQMSSRRNSAANIANQIPTSDCQTPFTQYKEMCVMNNYFGIGLDAKIALEFHNKREESEKTRSRSKLFMWYGILGGKELMHRTYRNLEQRIKLECDGVPIDLPSLQGIVILNIPSYSGGANFWGRNKDGPEFTVQSFDDRILEVVALFGVIHVATSRVPNAVRLQNHRIAQCRHVRIVILGDEPIPVQVDGEPWLQPPGIMQIVHKNRAQMLARNPVFDATLKKWEEQKEKASTTTAPSTPTALGAPTSDIISFLTRAREFLRLIEGEIARLGVSSVLLQSLDDANAIVKGGASGEEDPEAIDDLEEREETVASVERVVELLEDHFGFPERTRAPVGQPKLGPTPHFNFEIHGDDPDHWRYIINSIRQDMDREEAAIREARKQDLIAGKPKRSRFTAWFHKRFGTRSAPFAFANIPYWTSEEVCAWLSSIGMSEYGSTFRKNDIQGSELMHLERSDIMDIGITKIGHVKRLQSAIVDLRAQNQRARRAQARKKRVAKDYKPDHSGPSGAGGAVDKRKESSAERYADGQPSTSSTGATTSSGKTRRIPPKDGESAPAVMQMDPASTDC

Target ORF information:

RefSeq Version	NM_069075.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Diacylglycerol kinase (dgk-4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_069075.4

ORF Insert Sequence:

ATGGTGGTGA CGCGTGGATT GGACAATGAT GTCGACGACG AACGAGAAGC GTGGAAACAG 
TCGATTCTGC GTGCGAAAGA TGTCATGCCG GAAAAGGCGT CTGGACCACT CGTTGCATTA 
CTTGAAGGCA CCTCGGGTCA TCATCACTGG ACAGAGACAA ATCATCCTCG GCCAACGTTT 
TGCAATTATT GCCGCGAAAA GTTGAACGGA GTCCCATGGC ACGGCTATAC GTGTGATATC 
TGCAAAGTGA AAGCACATCG AAAATGCCGT GACAATTTGA CGGAGCAATG CAAATGGACA 
AATCAATCAA GTATTCCACA ACATTTACAA TTTATTAGCC CAGAGAATTC AATTCTACCT 
CATCAATGGA TGGAAGGAAA TCTTCCAATG CCATCAAAAT GCTCAGTTTG TGAAAAGCCA 
TGTGGTTCCG TTAGAAAACT AATCGACTAT CGATGTATTT GGTGTGGATG TTGTGTCCAT 
GATACGTGTA TTGGAAATCT GGCACGAGCA TGTAGTTTAG GTCACTCGGC ACTTTCAGTA 
ATATCACCAT TAGCAATGAA AGAAGTTACT CCAAATGGGA CAGCTGTATT ACGAGAAGAA 
GCGTACGGTG GTGATTCTCC AGGCGGCAGT CCACTAATTG TACTAATCAA CGCGAAATCG 
GGTGATAGTC AGGGTCAAAG AATTATCAAA AAATTTCGAC GGATTTTGAA TCCAATTCAA 
GTTTTCGATA TTATCGCAAC TGGTCCCGAT TTTGCACTAA CATTTTTCTC GCATCTTGAA 
TCATTTCGTG TCCTTGTATG TGGTGGAGAT GGAACTGTTG GATGGGTTTT AAGCGCTTTT 
GATAGACTGA ATTTACACTC AAAGTGTCAA TTAGCAATTC TTCCACTTGG AACTGGAAAT 
GATTTGGCTC GTGTACTTGG TTGGGGACAT GCTTTTTATG ATGATACACT ACTTCCACAA 
GTTGTTCGAA CAATGGAAAG AGCACATACA AAAATGCTAG ATCGGTGGAG TGTTCTCGCT 
ATTGAAGGCC CTCAAGCAGA TGCGGTTCGT CGGTACGAGG AGCTGGTCAT CGAAAAAGTT 
CGAACGTTTC TAGAAGCTGA ACAAACATGG GACATTATAC ATGCAGCTAA TCAACTATGT 
ACAACGATTC GGGACCTTGT TCATAGTGTA TCACATACGT ATTCTGATTT AGAAAATAAC 
AAGTTGAAGC TTGATGACGA GTCAAGACTT GGAGGAAGTG ATACAATGAC GGAAAATTGC 
AATACTCTAC TGGGAAAACT CGATCGGTTG ATGAAATCAC TGAAAGAAGA CGCGAATTGT 
TTGGAGGAGT CTGGATTTGA GCCTGGTGAT GATGATGATT GTTCAACAGG AGACGAAATG 
CTCAAAAAAC GAGATTCTGT AGTAAATCGA GCGAATAGTC TTAAAAAGGC ATTCCGAGAA 
GTCATTTCAA TGGCCGAAAA GGGTATTGAT CAACACTATC GAGATCCGAA TACGGCGTGT 
ACGAAAAGAG AGAGGTTCAG GAAGAAGAGA AGTAAAACTA CGCCTAGCGT TCTTACGATA 
TCCCATTCCA ACCTGTCTTC TTCATCAGCC TGTTCCCCAC CGTGTTCACC CGCAGGAGAA 
GATCCGCCAC AAGATGATCA AAAAGAGTTT CGTAGTGCAT CTTGTAAAGC AACATTTGAA 
ATCAGTCATT CTCAATCAAG TTCATCAGCT GTTCTAACAG CACTATTCAA TGCACAATCT 
GCACGAACCT ATGATGAATT AGAGAAACCA AGTATAGGAA CAGTACATCC ACCAACTCCT 
GGAGCAACTC GTGAGCCATC GACTGCTTAT GATGATGATG AGGAAAATGA AAATGTCGAG 
AATCGAAAAG AAATTGATCA AAAATCGAAT AGTAAAAAGT CTTCAGTATC AACACCAGAA 
GATATTACAA AAGGATCACC TGATCGGCCG AGGATCTATT CTGATACCAC ACTAAACAAA 
AATATGGAGA AAATGAAAAG TCAAAGCTTG CATCCTATTT GTTCAAGTGC TGACAAGATG 
AAGCAGAGTC ACTCGGATAG TAGCTTATAT GCAGACTACG AGCATTTGGA AGGTTCATCA 
TCTGGTTGTG GTGCCGGTGC TTCAGGAAGT ACATTATCGC CTGCTCGGGC TTCTGATTCT 
GCCACCTGGT CGCGCATTAA AGAACGTAAA CGAACTGTTG GAAGTGATTT GGGATTGTCT 
AGTAGCTCTC ATTTGAGAAG TTGTCGTAAC ATGCTCTCCG GATTCGCCGG TGGATCTCTC 
ATTGCTGAAA TTTTACTATT AAATGCACGA GTACTGTCAC AGATGTCGTC ACGTCGCAAC 
TCAGCGGCAA ATATTGCAAA TCAAATTCCA ACGTCCGACT GTCAAACACC ATTCACTCAA 
TACAAGGAAA TGTGTGTAAT GAACAACTAT TTTGGAATTG GTCTTGACGC GAAAATCGCT 
CTGGAATTCC ACAACAAACG AGAAGAGTCT GAAAAAACGC GGAGCCGATC GAAACTCTTC 
ATGTGGTACG GTATTCTCGG AGGAAAAGAG CTGATGCATC GTACCTACCG TAATCTCGAG 
CAAAGGATCA AATTGGAATG TGATGGAGTT CCGATCGATC TTCCATCTCT TCAGGGAATC 
GTAATTTTGA ATATTCCATC ATATTCGGGC GGTGCAAACT TCTGGGGTCG GAACAAGGAT 
GGTCCAGAAT TCACAGTTCA AAGTTTCGAT GATCGAATTT TGGAGGTAGT TGCACTGTTC 
GGCGTGATCC ACGTGGCAAC GAGTCGTGTC CCAAATGCAG TTCGTCTCCA AAACCATCGA 
ATTGCTCAAT GTCGACATGT TCGAATTGTG ATACTCGGAG ACGAACCAAT CCCAGTTCAA 
GTTGACGGTG AACCTTGGCT TCAACCTCCT GGAATTATGC AAATTGTTCA CAAAAATCGA 
GCTCAGATGC TTGCCAGAAA TCCAGTTTTT GATGCAACAC TTAAGAAATG GGAAGAACAA 
AAAGAGAAGG CTTCCACGAC AACAGCACCA TCCACACCTA CGGCTCTTGG AGCTCCCACT 
TCAGATATTA TTTCGTTCTT GACGAGAGCT CGCGAATTTT TGAGATTGAT TGAAGGTGAA 
ATAGCGAGAT TAGGGGTTTC ATCGGTACTT TTGCAATCCT TGGATGATGC AAATGCTATT 
GTCAAAGGTG GAGCATCAGG AGAAGAAGAT CCAGAGGCTA TCGATGACTT AGAAGAACGA 
GAAGAAACAG TTGCATCGGT GGAAAGAGTC GTTGAACTTC TTGAGGATCA TTTTGGTTTT 
CCTGAAAGAA CAAGAGCACC AGTTGGGCAA CCAAAACTAG GACCCACTCC GCATTTTAAT 
TTTGAAATTC ATGGTGACGA TCCTGATCAT TGGCGTTATA TAATCAACTC GATTCGTCAG 
GATATGGATC GAGAAGAAGC AGCAATTAGA GAAGCTAGAA AACAAGATTT AATAGCTGGA 
AAGCCAAAAA GATCCCGATT CACTGCATGG TTTCACAAAC GATTTGGAAC TAGATCTGCA 
CCATTTGCAT TCGCTAATAT TCCATACTGG ACGAGCGAAG AAGTATGTGC ATGGCTCTCT 
TCAATTGGAA TGTCTGAATA TGGATCAACG TTCCGAAAGA ATGATATTCA AGGTTCTGAA 
TTGATGCATT TAGAACGATC CGATATTATG GATATTGGGA TCACAAAAAT TGGACATGTG 
AAGAGGTTGC AGTCCGCCAT TGTCGACCTC CGAGCTCAAA ACCAACGTGC ACGTCGCGCT 
CAGGCTCGTA AAAAACGTGT CGCCAAAGAC TATAAACCCG ATCATTCTGG TCCTTCTGGT 
GCTGGCGGTG CAGTAGATAA ACGAAAAGAA TCAAGTGCTG AAAGATATGC AGATGGACAA 
CCATCAACAT CATCTACGGG AGCTACCACT TCATCTGGAA AAACACGGAG AATTCCACCG 
AAAGATGGAG AATCCGCGCC TGCTGTCATG CAAATGGATC CAGCTAGTAC AGATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgk-4 ( NM_069076.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_501477.2 Caenorhabditis elegans Diacylglycerol kinase (dgk-4), partial mRNA.

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CloneID	OCa343747
Clone ID Related Accession (Same CDS sequence)	NM_069076.2 , NM_069076.3
Accession Version	NM_069076.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Diacylglycerol kinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_069076.1. COMPLETENESS: incomplete on both ends.

ATGGTGGTGA CGCGTGGATT GGACAATGAT GTCGACGACG AACGAGAAGC GTGGAAACAG 
TCGATTCTGC GTGCGAAAGA TGTCATGCCG GAAAAGGCGT CTGGACCACT CGTTGCATTA 
CTTGAAGGCA CCTCGGGTCA TCATCACTGG ACAGAGACAA ATCATCCTCG GCCAACGTTT 
TGCAATTATT GCCGCGAAAA GTTGAACGGA GTCCCATGGC ACGGCTATAC GTGTGATATC 
TGCAAAGTGA AAGCACATCG AAAATGCCGT GACAATTTGA CGGAGCAATG CAAATGGACA 
AATCAATCAA GTATTCCACA ACATTTACAA TTTATTAGCC CAGAGAATTC AATTCTACCT 
CATCAATGGA TGGAAGGAAA TCTTCCAATG CCATCAAAAT GCTCAGTTTG TGAAAAGCCA 
TGTGGTTCCG TTAGAAAACT AATCGACTAT CGATGTATTT GGTGTGGATG TTGTGTCCAT 
GATACGTGTA TTGGAAATCT GGCACGAGCA TGTAGTTTAG GTCACTCGGC ACTTTCAGTA 
ATATCACCAT TAGCAATGAA AGAAGTTACT CCAAATGGGA CAGCTGTATT ACGAGAAGAA 
GCGTACGGTG GTGATTCTCC AGGCGGCAGT CCACTAATTG TACTAATCAA CGCGAAATCG 
GGTGATAGTC AGGGTCAAAG AATTATCAAA AAATTTCGAC GGATTTTGAA TCCAATTCAA 
GTTTTCGATA TTATCGCAAC TGGTCCCGAT TTTGCACTAA CATTTTTCTC GCATCTTGAA 
TCATTTCGTG TCCTTGTATG TGGTGGAGAT GGAACTGTTG GATGGGTTTT AAGCGCTTTT 
GATAGACTGA ATTTACACTC AAAGTGTCAA TTAGCAATTC TTCCACTTGG AACTGGAAAT 
GATTTGGCTC GTGTACTTGG TTGGGGACAT GCTTTTTATG ATGATACACT ACTTCCACAA 
GTTGTTCGAA CAATGGAAAG AGCACATACA AAAATGCTAG ATCGGTGGAG TGTTCTCGCT 
ATTGAAGGCC CTCAAGCAGA TGCGGTTCGT CGGTACGAGG AGCTGGTCAT CGAAAAAGTT 
CGAACGTTTC TAGAAGCTGA ACAAACATGG GACATTATAC ATGCAGCTAA TCAACTATGT 
ACAACGATTC GGGACCTTGT TCATAGTGTA TCACATACGT ATTCTGATTT AGAAAATAAC 
AAGTTGAAGC TTGATGACGA GTCAAGACTT GGAGGAAGTG ATACAATGAC GGAAAATTGC 
AATACTCTAC TGGGAAAACT CGATCGGTTG ATGAAATCAC TGAAAGAAGA CGCGAATTGT 
TTGGAGGAGT CTGGATTTGA GCCTGGTGAT GATGATGATT GTTCAACAGG AGACGAAATG 
CTCAAAAAAC GAGATTCTGT AGTAAATCGA GCGAATAGTC TTAAAAAGGC ATTCCGAGAA 
GTCATTTCAA TGGCCGAAAA GGGTATTGAT CAACACTATC GAGATCCGAA TACGGCGTGT 
ACGAAAAGAG AGAGGTTCAG GAAGAAGAGA AGTAAAACTA CGCCTAGCGT TCTTACGATA 
TCCCATTCCA ACCTGTCTTC TTCATCAGCC TGTTCCCCAC CGTGTTCACC CGCAGGAGAA 
GATCCGCCAC AAGATGATCA AAAAGAGTTT CGTAGTGCAT CTTGTAAAGC AACATTTGAA 
ATCAGTCATT CTCAATCAAG TTCATCAGCT GTTCTAACAG CACTATTCAA TGCACAATCT 
GCACGAACCT ATGATGAATT AGAGAAACCA AGTATAGGAA CAGTACATCC ACCAACTCCT 
GGAGCAACTC GTGAGCCATC GACTGCTTAT GATGATGATG AGGAAAATGA AAATGTCGAG 
AATCGAAAAG AAATTGATCA AAAATCGAAT AGTAAAAAGT CTTCAGTATC AACACCAGAA 
GATATTACAA AAGGATCACC TGATCGGCCG AGGATCTATT CTGATACCAC ACTAAACAAA 
AATATGGAGA AAATGAAAAG TCAAAGCTTG CATCCTATTT GTTCAAGTGC TGACAAGATG 
AAGCAGAGTC ACTCGGATAG TAGCTTATAT GCAGACTACG AGCATTTGGA AGGTTCATCA 
TCTGGTTGTG GTGCCGGTGC TTCAGGAAGT ACATTATCGC CTGCTCGGGC TTCTGATTCT 
GCCACCTGGT CGCGCATTAA AGAACGTAAA CGAACTGTTG GAAGTGATTT GGGATTGTCT 
AGTAGCTCTC ATTTGAGAAG CACTCAATAC AAGGAAATGT GTGTAATGAA CAACTATTTT 
GGAATTGGTC TTGACGCGAA AATCGCTCTG GAATTCCACA ACAAACGAGA AGAGTCTGAA 
AAAACGCGGA GCCGATCGAA ACTCTTCATG TGGTACGGTA TTCTCGGAGG AAAAGAGCTG 
ATGCATCGTA CCTACCGTAA TCTCGAGCAA AGGATCAAAT TGGAATGTGA TGGAGTTCCG 
ATCGATCTTC CATCTCTTCA GGGAATCGTA ATTTTGAATA TTCCATCATA TTCGGGCGGT 
GCAAACTTCT GGGGTCGGAA CAAGGATGGT CCAGAATTCA CAGTTCAAAG TTTCGATGAT 
CGAATTTTGG AGGTAGTTGC ACTGTTCGGC GTGATCCACG TGGCAACGAG TCGTGTCCCA 
AATGCAGTTC GTCTCCAAAA CCATCGAATT GCTCAATGTC GACATGTTCG AATTGTGATA 
CTCGGAGACG AACCAATCCC AGTTCAAGTT GACGGTGAAC CTTGGCTTCA ACCTCCTGGA 
ATTATGCAAA TTGTTCACAA AAATCGAGCT CAGATGCTTG CCAGAAATCC AGTTTTTGAT 
GCAACACTTA AGAAATGGGA AGAACAAAAA GAGAAGGCTT CCACGACAAC AGCACCATCC 
ACACCTACGG CTCTTGGAGC TCCCACTTCA GATATTATTT CGTTCTTGAC GAGAGCTCGC 
GAATTTTTGA GATTGATTGA AGGTGAAATA GCGAGATTAG GGGTTTCATC GGTACTTTTG 
CAATCCTTGG ATGATGCAAA TGCTATTGTC AAAGGTGGAG CATCAGGAGA AGAAGATCCA 
GAGGCTATCG ATGACTTAGA AGAACGAGAA GAAACAGTTG CATCGGTGGA AAGAGTCGTT 
GAACTTCTTG AGGATCATTT TGGTTTTCCT GAAAGAACAA GAGCACCAGT TGGGCAACCA 
AAACTAGGAC CCACTCCGCA TTTTAATTTT GAAATTCATG GTGACGATCC TGATCATTGG 
CGTTATATAA TCAACTCGAT TCGTCAGGAT ATGGATCGAG AAGAAGCAGC AATTAGAGAA 
GCTAGAAAAC AAGATTTAAT AGCTGGAAAG CCAAAAAGAT CCCGATTCAC TGCATGGTTT 
CACAAACGAT TTGGAACTAG ATCTGCACCA TTTGCATTCG CTAATATTCC ATACTGGACG 
AGCGAAGAAG TATGTGCATG GCTCTCTTCA ATTGGAATGT CTGAATATGG ATCAACGTTC 
CGAAAGAATG ATATTCAAGG TTCTGAATTG ATGCATTTAG AACGATCCGA TATTATGGAT 
ATTGGGATCA CAAAAATTGG ACATGTGAAG AGGTTGCAGT CCGCCATTGT CGACCTCCGA 
GCTCAAAACC AACGTGCACG TCGCGCTCAG GCTCGTAAAA AACGTGTCGC CAAAGACTAT 
AAACCCGATC ATTCTGGTCC TTCTGGTGCT GGCGGTGCAG TAGATAAACG AAAAGAATCA 
AGTGCTGAAA GATATGCAGA TGGACAACCA TCAACATCAT CTACGGGAGC TACCACTTCA 
TCTGGAAAAA CACGGAGAAT TCCACCGAAA GATGGAGAAT CCGCGCCTGC TGTCATGCAA 
ATGGATCCAG CTAGTACAGA TTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_501477.2
CDS	1..3867
Translation	MVVTRGLDNDVDDEREAWKQSILRAKDVMPEKASGPLVALLEGTSGHHHWTETNHPRPTFCNYCREKLNGVPWHGYTCDICKVKAHRKCRDNLTEQCKWTNQSSIPQHLQFISPENSILPHQWMEGNLPMPSKCSVCEKPCGSVRKLIDYRCIWCGCCVHDTCIGNLARACSLGHSALSVISPLAMKEVTPNGTAVLREEAYGGDSPGGSPLIVLINAKSGDSQGQRIIKKFRRILNPIQVFDIIATGPDFALTFFSHLESFRVLVCGGDGTVGWVLSAFDRLNLHSKCQLAILPLGTGNDLARVLGWGHAFYDDTLLPQVVRTMERAHTKMLDRWSVLAIEGPQADAVRRYEELVIEKVRTFLEAEQTWDIIHAANQLCTTIRDLVHSVSHTYSDLENNKLKLDDESRLGGSDTMTENCNTLLGKLDRLMKSLKEDANCLEESGFEPGDDDDCSTGDEMLKKRDSVVNRANSLKKAFREVISMAEKGIDQHYRDPNTACTKRERFRKKRSKTTPSVLTISHSNLSSSSACSPPCSPAGEDPPQDDQKEFRSASCKATFEISHSQSSSSAVLTALFNAQSARTYDELEKPSIGTVHPPTPGATREPSTAYDDDEENENVENRKEIDQKSNSKKSSVSTPEDITKGSPDRPRIYSDTTLNKNMEKMKSQSLHPICSSADKMKQSHSDSSLYADYEHLEGSSSGCGAGASGSTLSPARASDSATWSRIKERKRTVGSDLGLSSSSHLRSTQYKEMCVMNNYFGIGLDAKIALEFHNKREESEKTRSRSKLFMWYGILGGKELMHRTYRNLEQRIKLECDGVPIDLPSLQGIVILNIPSYSGGANFWGRNKDGPEFTVQSFDDRILEVVALFGVIHVATSRVPNAVRLQNHRIAQCRHVRIVILGDEPIPVQVDGEPWLQPPGIMQIVHKNRAQMLARNPVFDATLKKWEEQKEKASTTTAPSTPTALGAPTSDIISFLTRAREFLRLIEGEIARLGVSSVLLQSLDDANAIVKGGASGEEDPEAIDDLEEREETVASVERVVELLEDHFGFPERTRAPVGQPKLGPTPHFNFEIHGDDPDHWRYIINSIRQDMDREEAAIREARKQDLIAGKPKRSRFTAWFHKRFGTRSAPFAFANIPYWTSEEVCAWLSSIGMSEYGSTFRKNDIQGSELMHLERSDIMDIGITKIGHVKRLQSAIVDLRAQNQRARRAQARKKRVAKDYKPDHSGPSGAGGAVDKRKESSAERYADGQPSTSSTGATTSSGKTRRIPPKDGESAPAVMQMDPASTDC

Target ORF information:

RefSeq Version	NM_069076.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Diacylglycerol kinase (dgk-4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_069076.2

ORF Insert Sequence:

ATGGTGGTGA CGCGTGGATT GGACAATGAT GTCGACGACG AACGAGAAGC GTGGAAACAG 
TCGATTCTGC GTGCGAAAGA TGTCATGCCG GAAAAGGCGT CTGGACCACT CGTTGCATTA 
CTTGAAGGCA CCTCGGGTCA TCATCACTGG ACAGAGACAA ATCATCCTCG GCCAACGTTT 
TGCAATTATT GCCGCGAAAA GTTGAACGGA GTCCCATGGC ACGGCTATAC GTGTGATATC 
TGCAAAGTGA AAGCACATCG AAAATGCCGT GACAATTTGA CGGAGCAATG CAAATGGACA 
AATCAATCAA GTATTCCACA ACATTTACAA TTTATTAGCC CAGAGAATTC AATTCTACCT 
CATCAATGGA TGGAAGGAAA TCTTCCAATG CCATCAAAAT GCTCAGTTTG TGAAAAGCCA 
TGTGGTTCCG TTAGAAAACT AATCGACTAT CGATGTATTT GGTGTGGATG TTGTGTCCAT 
GATACGTGTA TTGGAAATCT GGCACGAGCA TGTAGTTTAG GTCACTCGGC ACTTTCAGTA 
ATATCACCAT TAGCAATGAA AGAAGTTACT CCAAATGGGA CAGCTGTATT ACGAGAAGAA 
GCGTACGGTG GTGATTCTCC AGGCGGCAGT CCACTAATTG TACTAATCAA CGCGAAATCG 
GGTGATAGTC AGGGTCAAAG AATTATCAAA AAATTTCGAC GGATTTTGAA TCCAATTCAA 
GTTTTCGATA TTATCGCAAC TGGTCCCGAT TTTGCACTAA CATTTTTCTC GCATCTTGAA 
TCATTTCGTG TCCTTGTATG TGGTGGAGAT GGAACTGTTG GATGGGTTTT AAGCGCTTTT 
GATAGACTGA ATTTACACTC AAAGTGTCAA TTAGCAATTC TTCCACTTGG AACTGGAAAT 
GATTTGGCTC GTGTACTTGG TTGGGGACAT GCTTTTTATG ATGATACACT ACTTCCACAA 
GTTGTTCGAA CAATGGAAAG AGCACATACA AAAATGCTAG ATCGGTGGAG TGTTCTCGCT 
ATTGAAGGCC CTCAAGCAGA TGCGGTTCGT CGGTACGAGG AGCTGGTCAT CGAAAAAGTT 
CGAACGTTTC TAGAAGCTGA ACAAACATGG GACATTATAC ATGCAGCTAA TCAACTATGT 
ACAACGATTC GGGACCTTGT TCATAGTGTA TCACATACGT ATTCTGATTT AGAAAATAAC 
AAGTTGAAGC TTGATGACGA GTCAAGACTT GGAGGAAGTG ATACAATGAC GGAAAATTGC 
AATACTCTAC TGGGAAAACT CGATCGGTTG ATGAAATCAC TGAAAGAAGA CGCGAATTGT 
TTGGAGGAGT CTGGATTTGA GCCTGGTGAT GATGATGATT GTTCAACAGG AGACGAAATG 
CTCAAAAAAC GAGATTCTGT AGTAAATCGA GCGAATAGTC TTAAAAAGGC ATTCCGAGAA 
GTCATTTCAA TGGCCGAAAA GGGTATTGAT CAACACTATC GAGATCCGAA TACGGCGTGT 
ACGAAAAGAG AGAGGTTCAG GAAGAAGAGA AGTAAAACTA CGCCTAGCGT TCTTACGATA 
TCCCATTCCA ACCTGTCTTC TTCATCAGCC TGTTCCCCAC CGTGTTCACC CGCAGGAGAA 
GATCCGCCAC AAGATGATCA AAAAGAGTTT CGTAGTGCAT CTTGTAAAGC AACATTTGAA 
ATCAGTCATT CTCAATCAAG TTCATCAGCT GTTCTAACAG CACTATTCAA TGCACAATCT 
GCACGAACCT ATGATGAATT AGAGAAACCA AGTATAGGAA CAGTACATCC ACCAACTCCT 
GGAGCAACTC GTGAGCCATC GACTGCTTAT GATGATGATG AGGAAAATGA AAATGTCGAG 
AATCGAAAAG AAATTGATCA AAAATCGAAT AGTAAAAAGT CTTCAGTATC AACACCAGAA 
GATATTACAA AAGGATCACC TGATCGGCCG AGGATCTATT CTGATACCAC ACTAAACAAA 
AATATGGAGA AAATGAAAAG TCAAAGCTTG CATCCTATTT GTTCAAGTGC TGACAAGATG 
AAGCAGAGTC ACTCGGATAG TAGCTTATAT GCAGACTACG AGCATTTGGA AGGTTCATCA 
TCTGGTTGTG GTGCCGGTGC TTCAGGAAGT ACATTATCGC CTGCTCGGGC TTCTGATTCT 
GCCACCTGGT CGCGCATTAA AGAACGTAAA CGAACTGTTG GAAGTGATTT GGGATTGTCT 
AGTAGCTCTC ATTTGAGAAG CACTCAATAC AAGGAAATGT GTGTAATGAA CAACTATTTT 
GGAATTGGTC TTGACGCGAA AATCGCTCTG GAATTCCACA ACAAACGAGA AGAGTCTGAA 
AAAACGCGGA GCCGATCGAA ACTCTTCATG TGGTACGGTA TTCTCGGAGG AAAAGAGCTG 
ATGCATCGTA CCTACCGTAA TCTCGAGCAA AGGATCAAAT TGGAATGTGA TGGAGTTCCG 
ATCGATCTTC CATCTCTTCA GGGAATCGTA ATTTTGAATA TTCCATCATA TTCGGGCGGT 
GCAAACTTCT GGGGTCGGAA CAAGGATGGT CCAGAATTCA CAGTTCAAAG TTTCGATGAT 
CGAATTTTGG AGGTAGTTGC ACTGTTCGGC GTGATCCACG TGGCAACGAG TCGTGTCCCA 
AATGCAGTTC GTCTCCAAAA CCATCGAATT GCTCAATGTC GACATGTTCG AATTGTGATA 
CTCGGAGACG AACCAATCCC AGTTCAAGTT GACGGTGAAC CTTGGCTTCA ACCTCCTGGA 
ATTATGCAAA TTGTTCACAA AAATCGAGCT CAGATGCTTG CCAGAAATCC AGTTTTTGAT 
GCAACACTTA AGAAATGGGA AGAACAAAAA GAGAAGGCTT CCACGACAAC AGCACCATCC 
ACACCTACGG CTCTTGGAGC TCCCACTTCA GATATTATTT CGTTCTTGAC GAGAGCTCGC 
GAATTTTTGA GATTGATTGA AGGTGAAATA GCGAGATTAG GGGTTTCATC GGTACTTTTG 
CAATCCTTGG ATGATGCAAA TGCTATTGTC AAAGGTGGAG CATCAGGAGA AGAAGATCCA 
GAGGCTATCG ATGACTTAGA AGAACGAGAA GAAACAGTTG CATCGGTGGA AAGAGTCGTT 
GAACTTCTTG AGGATCATTT TGGTTTTCCT GAAAGAACAA GAGCACCAGT TGGGCAACCA 
AAACTAGGAC CCACTCCGCA TTTTAATTTT GAAATTCATG GTGACGATCC TGATCATTGG 
CGTTATATAA TCAACTCGAT TCGTCAGGAT ATGGATCGAG AAGAAGCAGC AATTAGAGAA 
GCTAGAAAAC AAGATTTAAT AGCTGGAAAG CCAAAAAGAT CCCGATTCAC TGCATGGTTT 
CACAAACGAT TTGGAACTAG ATCTGCACCA TTTGCATTCG CTAATATTCC ATACTGGACG 
AGCGAAGAAG TATGTGCATG GCTCTCTTCA ATTGGAATGT CTGAATATGG ATCAACGTTC 
CGAAAGAATG ATATTCAAGG TTCTGAATTG ATGCATTTAG AACGATCCGA TATTATGGAT 
ATTGGGATCA CAAAAATTGG ACATGTGAAG AGGTTGCAGT CCGCCATTGT CGACCTCCGA 
GCTCAAAACC AACGTGCACG TCGCGCTCAG GCTCGTAAAA AACGTGTCGC CAAAGACTAT 
AAACCCGATC ATTCTGGTCC TTCTGGTGCT GGCGGTGCAG TAGATAAACG AAAAGAATCA 
AGTGCTGAAA GATATGCAGA TGGACAACCA TCAACATCAT CTACGGGAGC TACCACTTCA 
TCTGGAAAAA CACGGAGAAT TCCACCGAAA GATGGAGAAT CCGCGCCTGC TGTCATGCAA 
ATGGATCCAG CTAGTACAGA TTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgk-4 ( NM_069076.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_501477.2 Caenorhabditis elegans Diacylglycerol kinase (dgk-4), mRNA.

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CloneID	OCa343747
Clone ID Related Accession (Same CDS sequence)	NM_069076.2 , NM_069076.3
Accession Version	NM_069076.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Diacylglycerol kinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_069076.2.

ATGGTGGTGA CGCGTGGATT GGACAATGAT GTCGACGACG AACGAGAAGC GTGGAAACAG 
TCGATTCTGC GTGCGAAAGA TGTCATGCCG GAAAAGGCGT CTGGACCACT CGTTGCATTA 
CTTGAAGGCA CCTCGGGTCA TCATCACTGG ACAGAGACAA ATCATCCTCG GCCAACGTTT 
TGCAATTATT GCCGCGAAAA GTTGAACGGA GTCCCATGGC ACGGCTATAC GTGTGATATC 
TGCAAAGTGA AAGCACATCG AAAATGCCGT GACAATTTGA CGGAGCAATG CAAATGGACA 
AATCAATCAA GTATTCCACA ACATTTACAA TTTATTAGCC CAGAGAATTC AATTCTACCT 
CATCAATGGA TGGAAGGAAA TCTTCCAATG CCATCAAAAT GCTCAGTTTG TGAAAAGCCA 
TGTGGTTCCG TTAGAAAACT AATCGACTAT CGATGTATTT GGTGTGGATG TTGTGTCCAT 
GATACGTGTA TTGGAAATCT GGCACGAGCA TGTAGTTTAG GTCACTCGGC ACTTTCAGTA 
ATATCACCAT TAGCAATGAA AGAAGTTACT CCAAATGGGA CAGCTGTATT ACGAGAAGAA 
GCGTACGGTG GTGATTCTCC AGGCGGCAGT CCACTAATTG TACTAATCAA CGCGAAATCG 
GGTGATAGTC AGGGTCAAAG AATTATCAAA AAATTTCGAC GGATTTTGAA TCCAATTCAA 
GTTTTCGATA TTATCGCAAC TGGTCCCGAT TTTGCACTAA CATTTTTCTC GCATCTTGAA 
TCATTTCGTG TCCTTGTATG TGGTGGAGAT GGAACTGTTG GATGGGTTTT AAGCGCTTTT 
GATAGACTGA ATTTACACTC AAAGTGTCAA TTAGCAATTC TTCCACTTGG AACTGGAAAT 
GATTTGGCTC GTGTACTTGG TTGGGGACAT GCTTTTTATG ATGATACACT ACTTCCACAA 
GTTGTTCGAA CAATGGAAAG AGCACATACA AAAATGCTAG ATCGGTGGAG TGTTCTCGCT 
ATTGAAGGCC CTCAAGCAGA TGCGGTTCGT CGGTACGAGG AGCTGGTCAT CGAAAAAGTT 
CGAACGTTTC TAGAAGCTGA ACAAACATGG GACATTATAC ATGCAGCTAA TCAACTATGT 
ACAACGATTC GGGACCTTGT TCATAGTGTA TCACATACGT ATTCTGATTT AGAAAATAAC 
AAGTTGAAGC TTGATGACGA GTCAAGACTT GGAGGAAGTG ATACAATGAC GGAAAATTGC 
AATACTCTAC TGGGAAAACT CGATCGGTTG ATGAAATCAC TGAAAGAAGA CGCGAATTGT 
TTGGAGGAGT CTGGATTTGA GCCTGGTGAT GATGATGATT GTTCAACAGG AGACGAAATG 
CTCAAAAAAC GAGATTCTGT AGTAAATCGA GCGAATAGTC TTAAAAAGGC ATTCCGAGAA 
GTCATTTCAA TGGCCGAAAA GGGTATTGAT CAACACTATC GAGATCCGAA TACGGCGTGT 
ACGAAAAGAG AGAGGTTCAG GAAGAAGAGA AGTAAAACTA CGCCTAGCGT TCTTACGATA 
TCCCATTCCA ACCTGTCTTC TTCATCAGCC TGTTCCCCAC CGTGTTCACC CGCAGGAGAA 
GATCCGCCAC AAGATGATCA AAAAGAGTTT CGTAGTGCAT CTTGTAAAGC AACATTTGAA 
ATCAGTCATT CTCAATCAAG TTCATCAGCT GTTCTAACAG CACTATTCAA TGCACAATCT 
GCACGAACCT ATGATGAATT AGAGAAACCA AGTATAGGAA CAGTACATCC ACCAACTCCT 
GGAGCAACTC GTGAGCCATC GACTGCTTAT GATGATGATG AGGAAAATGA AAATGTCGAG 
AATCGAAAAG AAATTGATCA AAAATCGAAT AGTAAAAAGT CTTCAGTATC AACACCAGAA 
GATATTACAA AAGGATCACC TGATCGGCCG AGGATCTATT CTGATACCAC ACTAAACAAA 
AATATGGAGA AAATGAAAAG TCAAAGCTTG CATCCTATTT GTTCAAGTGC TGACAAGATG 
AAGCAGAGTC ACTCGGATAG TAGCTTATAT GCAGACTACG AGCATTTGGA AGGTTCATCA 
TCTGGTTGTG GTGCCGGTGC TTCAGGAAGT ACATTATCGC CTGCTCGGGC TTCTGATTCT 
GCCACCTGGT CGCGCATTAA AGAACGTAAA CGAACTGTTG GAAGTGATTT GGGATTGTCT 
AGTAGCTCTC ATTTGAGAAG CACTCAATAC AAGGAAATGT GTGTAATGAA CAACTATTTT 
GGAATTGGTC TTGACGCGAA AATCGCTCTG GAATTCCACA ACAAACGAGA AGAGTCTGAA 
AAAACGCGGA GCCGATCGAA ACTCTTCATG TGGTACGGTA TTCTCGGAGG AAAAGAGCTG 
ATGCATCGTA CCTACCGTAA TCTCGAGCAA AGGATCAAAT TGGAATGTGA TGGAGTTCCG 
ATCGATCTTC CATCTCTTCA GGGAATCGTA ATTTTGAATA TTCCATCATA TTCGGGCGGT 
GCAAACTTCT GGGGTCGGAA CAAGGATGGT CCAGAATTCA CAGTTCAAAG TTTCGATGAT 
CGAATTTTGG AGGTAGTTGC ACTGTTCGGC GTGATCCACG TGGCAACGAG TCGTGTCCCA 
AATGCAGTTC GTCTCCAAAA CCATCGAATT GCTCAATGTC GACATGTTCG AATTGTGATA 
CTCGGAGACG AACCAATCCC AGTTCAAGTT GACGGTGAAC CTTGGCTTCA ACCTCCTGGA 
ATTATGCAAA TTGTTCACAA AAATCGAGCT CAGATGCTTG CCAGAAATCC AGTTTTTGAT 
GCAACACTTA AGAAATGGGA AGAACAAAAA GAGAAGGCTT CCACGACAAC AGCACCATCC 
ACACCTACGG CTCTTGGAGC TCCCACTTCA GATATTATTT CGTTCTTGAC GAGAGCTCGC 
GAATTTTTGA GATTGATTGA AGGTGAAATA GCGAGATTAG GGGTTTCATC GGTACTTTTG 
CAATCCTTGG ATGATGCAAA TGCTATTGTC AAAGGTGGAG CATCAGGAGA AGAAGATCCA 
GAGGCTATCG ATGACTTAGA AGAACGAGAA GAAACAGTTG CATCGGTGGA AAGAGTCGTT 
GAACTTCTTG AGGATCATTT TGGTTTTCCT GAAAGAACAA GAGCACCAGT TGGGCAACCA 
AAACTAGGAC CCACTCCGCA TTTTAATTTT GAAATTCATG GTGACGATCC TGATCATTGG 
CGTTATATAA TCAACTCGAT TCGTCAGGAT ATGGATCGAG AAGAAGCAGC AATTAGAGAA 
GCTAGAAAAC AAGATTTAAT AGCTGGAAAG CCAAAAAGAT CCCGATTCAC TGCATGGTTT 
CACAAACGAT TTGGAACTAG ATCTGCACCA TTTGCATTCG CTAATATTCC ATACTGGACG 
AGCGAAGAAG TATGTGCATG GCTCTCTTCA ATTGGAATGT CTGAATATGG ATCAACGTTC 
CGAAAGAATG ATATTCAAGG TTCTGAATTG ATGCATTTAG AACGATCCGA TATTATGGAT 
ATTGGGATCA CAAAAATTGG ACATGTGAAG AGGTTGCAGT CCGCCATTGT CGACCTCCGA 
GCTCAAAACC AACGTGCACG TCGCGCTCAG GCTCGTAAAA AACGTGTCGC CAAAGACTAT 
AAACCCGATC ATTCTGGTCC TTCTGGTGCT GGCGGTGCAG TAGATAAACG AAAAGAATCA 
AGTGCTGAAA GATATGCAGA TGGACAACCA TCAACATCAT CTACGGGAGC TACCACTTCA 
TCTGGAAAAA CACGGAGAAT TCCACCGAAA GATGGAGAAT CCGCGCCTGC TGTCATGCAA 
ATGGATCCAG CTAGTACAGA TTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_501477.2
CDS	100..3966
Translation	MVVTRGLDNDVDDEREAWKQSILRAKDVMPEKASGPLVALLEGTSGHHHWTETNHPRPTFCNYCREKLNGVPWHGYTCDICKVKAHRKCRDNLTEQCKWTNQSSIPQHLQFISPENSILPHQWMEGNLPMPSKCSVCEKPCGSVRKLIDYRCIWCGCCVHDTCIGNLARACSLGHSALSVISPLAMKEVTPNGTAVLREEAYGGDSPGGSPLIVLINAKSGDSQGQRIIKKFRRILNPIQVFDIIATGPDFALTFFSHLESFRVLVCGGDGTVGWVLSAFDRLNLHSKCQLAILPLGTGNDLARVLGWGHAFYDDTLLPQVVRTMERAHTKMLDRWSVLAIEGPQADAVRRYEELVIEKVRTFLEAEQTWDIIHAANQLCTTIRDLVHSVSHTYSDLENNKLKLDDESRLGGSDTMTENCNTLLGKLDRLMKSLKEDANCLEESGFEPGDDDDCSTGDEMLKKRDSVVNRANSLKKAFREVISMAEKGIDQHYRDPNTACTKRERFRKKRSKTTPSVLTISHSNLSSSSACSPPCSPAGEDPPQDDQKEFRSASCKATFEISHSQSSSSAVLTALFNAQSARTYDELEKPSIGTVHPPTPGATREPSTAYDDDEENENVENRKEIDQKSNSKKSSVSTPEDITKGSPDRPRIYSDTTLNKNMEKMKSQSLHPICSSADKMKQSHSDSSLYADYEHLEGSSSGCGAGASGSTLSPARASDSATWSRIKERKRTVGSDLGLSSSSHLRSTQYKEMCVMNNYFGIGLDAKIALEFHNKREESEKTRSRSKLFMWYGILGGKELMHRTYRNLEQRIKLECDGVPIDLPSLQGIVILNIPSYSGGANFWGRNKDGPEFTVQSFDDRILEVVALFGVIHVATSRVPNAVRLQNHRIAQCRHVRIVILGDEPIPVQVDGEPWLQPPGIMQIVHKNRAQMLARNPVFDATLKKWEEQKEKASTTTAPSTPTALGAPTSDIISFLTRAREFLRLIEGEIARLGVSSVLLQSLDDANAIVKGGASGEEDPEAIDDLEEREETVASVERVVELLEDHFGFPERTRAPVGQPKLGPTPHFNFEIHGDDPDHWRYIINSIRQDMDREEAAIREARKQDLIAGKPKRSRFTAWFHKRFGTRSAPFAFANIPYWTSEEVCAWLSSIGMSEYGSTFRKNDIQGSELMHLERSDIMDIGITKIGHVKRLQSAIVDLRAQNQRARRAQARKKRVAKDYKPDHSGPSGAGGAVDKRKESSAERYADGQPSTSSTGATTSSGKTRRIPPKDGESAPAVMQMDPASTDC

Target ORF information:

RefSeq Version	NM_069076.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Diacylglycerol kinase (dgk-4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_069076.3

ORF Insert Sequence:

ATGGTGGTGA CGCGTGGATT GGACAATGAT GTCGACGACG AACGAGAAGC GTGGAAACAG 
TCGATTCTGC GTGCGAAAGA TGTCATGCCG GAAAAGGCGT CTGGACCACT CGTTGCATTA 
CTTGAAGGCA CCTCGGGTCA TCATCACTGG ACAGAGACAA ATCATCCTCG GCCAACGTTT 
TGCAATTATT GCCGCGAAAA GTTGAACGGA GTCCCATGGC ACGGCTATAC GTGTGATATC 
TGCAAAGTGA AAGCACATCG AAAATGCCGT GACAATTTGA CGGAGCAATG CAAATGGACA 
AATCAATCAA GTATTCCACA ACATTTACAA TTTATTAGCC CAGAGAATTC AATTCTACCT 
CATCAATGGA TGGAAGGAAA TCTTCCAATG CCATCAAAAT GCTCAGTTTG TGAAAAGCCA 
TGTGGTTCCG TTAGAAAACT AATCGACTAT CGATGTATTT GGTGTGGATG TTGTGTCCAT 
GATACGTGTA TTGGAAATCT GGCACGAGCA TGTAGTTTAG GTCACTCGGC ACTTTCAGTA 
ATATCACCAT TAGCAATGAA AGAAGTTACT CCAAATGGGA CAGCTGTATT ACGAGAAGAA 
GCGTACGGTG GTGATTCTCC AGGCGGCAGT CCACTAATTG TACTAATCAA CGCGAAATCG 
GGTGATAGTC AGGGTCAAAG AATTATCAAA AAATTTCGAC GGATTTTGAA TCCAATTCAA 
GTTTTCGATA TTATCGCAAC TGGTCCCGAT TTTGCACTAA CATTTTTCTC GCATCTTGAA 
TCATTTCGTG TCCTTGTATG TGGTGGAGAT GGAACTGTTG GATGGGTTTT AAGCGCTTTT 
GATAGACTGA ATTTACACTC AAAGTGTCAA TTAGCAATTC TTCCACTTGG AACTGGAAAT 
GATTTGGCTC GTGTACTTGG TTGGGGACAT GCTTTTTATG ATGATACACT ACTTCCACAA 
GTTGTTCGAA CAATGGAAAG AGCACATACA AAAATGCTAG ATCGGTGGAG TGTTCTCGCT 
ATTGAAGGCC CTCAAGCAGA TGCGGTTCGT CGGTACGAGG AGCTGGTCAT CGAAAAAGTT 
CGAACGTTTC TAGAAGCTGA ACAAACATGG GACATTATAC ATGCAGCTAA TCAACTATGT 
ACAACGATTC GGGACCTTGT TCATAGTGTA TCACATACGT ATTCTGATTT AGAAAATAAC 
AAGTTGAAGC TTGATGACGA GTCAAGACTT GGAGGAAGTG ATACAATGAC GGAAAATTGC 
AATACTCTAC TGGGAAAACT CGATCGGTTG ATGAAATCAC TGAAAGAAGA CGCGAATTGT 
TTGGAGGAGT CTGGATTTGA GCCTGGTGAT GATGATGATT GTTCAACAGG AGACGAAATG 
CTCAAAAAAC GAGATTCTGT AGTAAATCGA GCGAATAGTC TTAAAAAGGC ATTCCGAGAA 
GTCATTTCAA TGGCCGAAAA GGGTATTGAT CAACACTATC GAGATCCGAA TACGGCGTGT 
ACGAAAAGAG AGAGGTTCAG GAAGAAGAGA AGTAAAACTA CGCCTAGCGT TCTTACGATA 
TCCCATTCCA ACCTGTCTTC TTCATCAGCC TGTTCCCCAC CGTGTTCACC CGCAGGAGAA 
GATCCGCCAC AAGATGATCA AAAAGAGTTT CGTAGTGCAT CTTGTAAAGC AACATTTGAA 
ATCAGTCATT CTCAATCAAG TTCATCAGCT GTTCTAACAG CACTATTCAA TGCACAATCT 
GCACGAACCT ATGATGAATT AGAGAAACCA AGTATAGGAA CAGTACATCC ACCAACTCCT 
GGAGCAACTC GTGAGCCATC GACTGCTTAT GATGATGATG AGGAAAATGA AAATGTCGAG 
AATCGAAAAG AAATTGATCA AAAATCGAAT AGTAAAAAGT CTTCAGTATC AACACCAGAA 
GATATTACAA AAGGATCACC TGATCGGCCG AGGATCTATT CTGATACCAC ACTAAACAAA 
AATATGGAGA AAATGAAAAG TCAAAGCTTG CATCCTATTT GTTCAAGTGC TGACAAGATG 
AAGCAGAGTC ACTCGGATAG TAGCTTATAT GCAGACTACG AGCATTTGGA AGGTTCATCA 
TCTGGTTGTG GTGCCGGTGC TTCAGGAAGT ACATTATCGC CTGCTCGGGC TTCTGATTCT 
GCCACCTGGT CGCGCATTAA AGAACGTAAA CGAACTGTTG GAAGTGATTT GGGATTGTCT 
AGTAGCTCTC ATTTGAGAAG CACTCAATAC AAGGAAATGT GTGTAATGAA CAACTATTTT 
GGAATTGGTC TTGACGCGAA AATCGCTCTG GAATTCCACA ACAAACGAGA AGAGTCTGAA 
AAAACGCGGA GCCGATCGAA ACTCTTCATG TGGTACGGTA TTCTCGGAGG AAAAGAGCTG 
ATGCATCGTA CCTACCGTAA TCTCGAGCAA AGGATCAAAT TGGAATGTGA TGGAGTTCCG 
ATCGATCTTC CATCTCTTCA GGGAATCGTA ATTTTGAATA TTCCATCATA TTCGGGCGGT 
GCAAACTTCT GGGGTCGGAA CAAGGATGGT CCAGAATTCA CAGTTCAAAG TTTCGATGAT 
CGAATTTTGG AGGTAGTTGC ACTGTTCGGC GTGATCCACG TGGCAACGAG TCGTGTCCCA 
AATGCAGTTC GTCTCCAAAA CCATCGAATT GCTCAATGTC GACATGTTCG AATTGTGATA 
CTCGGAGACG AACCAATCCC AGTTCAAGTT GACGGTGAAC CTTGGCTTCA ACCTCCTGGA 
ATTATGCAAA TTGTTCACAA AAATCGAGCT CAGATGCTTG CCAGAAATCC AGTTTTTGAT 
GCAACACTTA AGAAATGGGA AGAACAAAAA GAGAAGGCTT CCACGACAAC AGCACCATCC 
ACACCTACGG CTCTTGGAGC TCCCACTTCA GATATTATTT CGTTCTTGAC GAGAGCTCGC 
GAATTTTTGA GATTGATTGA AGGTGAAATA GCGAGATTAG GGGTTTCATC GGTACTTTTG 
CAATCCTTGG ATGATGCAAA TGCTATTGTC AAAGGTGGAG CATCAGGAGA AGAAGATCCA 
GAGGCTATCG ATGACTTAGA AGAACGAGAA GAAACAGTTG CATCGGTGGA AAGAGTCGTT 
GAACTTCTTG AGGATCATTT TGGTTTTCCT GAAAGAACAA GAGCACCAGT TGGGCAACCA 
AAACTAGGAC CCACTCCGCA TTTTAATTTT GAAATTCATG GTGACGATCC TGATCATTGG 
CGTTATATAA TCAACTCGAT TCGTCAGGAT ATGGATCGAG AAGAAGCAGC AATTAGAGAA 
GCTAGAAAAC AAGATTTAAT AGCTGGAAAG CCAAAAAGAT CCCGATTCAC TGCATGGTTT 
CACAAACGAT TTGGAACTAG ATCTGCACCA TTTGCATTCG CTAATATTCC ATACTGGACG 
AGCGAAGAAG TATGTGCATG GCTCTCTTCA ATTGGAATGT CTGAATATGG ATCAACGTTC 
CGAAAGAATG ATATTCAAGG TTCTGAATTG ATGCATTTAG AACGATCCGA TATTATGGAT 
ATTGGGATCA CAAAAATTGG ACATGTGAAG AGGTTGCAGT CCGCCATTGT CGACCTCCGA 
GCTCAAAACC AACGTGCACG TCGCGCTCAG GCTCGTAAAA AACGTGTCGC CAAAGACTAT 
AAACCCGATC ATTCTGGTCC TTCTGGTGCT GGCGGTGCAG TAGATAAACG AAAAGAATCA 
AGTGCTGAAA GATATGCAGA TGGACAACCA TCAACATCAT CTACGGGAGC TACCACTTCA 
TCTGGAAAAA CACGGAGAAT TCCACCGAAA GATGGAGAAT CCGCGCCTGC TGTCATGCAA 
ATGGATCCAG CTAGTACAGA TTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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dgk-4 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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