rrf-3 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	rrf-3
Entrez Gene ID	174315
Full Name	RNA-dependent RNA polymerase Family
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_063312.5	NP_495713.2	RNA-dependent RNA polymerase Family
NM_063312.6	NP_495713.2	RNA-dependent RNA polymerase Family

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	rrf-2	NP_493057.1
Caenorhabditis elegans (roundworm)	ego-1	NP_492132.1
Caenorhabditis elegans (roundworm)	rrf-3	NP_495713.2
Caenorhabditis elegans (roundworm)	rrf-1	NP_001250556.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results show that the tandem tudor protein ERI-5 potentiates ERI endo-RNAi by tethering an RNA-dependent RNA polymerase (RdRP, F10B5.7) module to DCR-1.
Title: Tudor domain ERI-5 tethers an RNA-dependent RNA polymerase to DCR-1 to potentiate endo-RNAi.

Present evidence for successive engagement of two different RdRPs (RRF-3/RRF-1) in an endogenous siRNA-based mechanism targeting specific mRNAs in C. elegans soma.
Title: Distinct phases of siRNA synthesis in an endogenous RNAi pathway in C. elegans soma.

the function of Caenorhabditis elegans RRF-3 during spermatogenesis
Title: A Caenorhabditis elegans RNA-directed RNA polymerase in sperm development and endogenous RNA interference.

These results provide additional support for the hypothesis that RRF-3 and ERI-1 act together in the endo-siRNA pathway, and furthermore suggests their involvement in additional biological processes.
Title: Whole genome microarray analysis of C. elegans rrf-3 and eri-1 mutants.

loss of this makes C. elegans hypersensitive to RNAi.
Title: Loss of the putative RNA-directed RNA polymerase RRF-3 makes C. elegans hypersensitive to RNAi.

The following rrf-3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rrf-3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa337517	NM_063312.6 Latest version!	Caenorhabditis elegans RNA-dependent RNA polymerase Family (rrf-3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OCa337517	NM_063312.5	Caenorhabditis elegans RNA-dependent RNA polymerase Family (rrf-3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rrf-3 ( NM_063312.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_495713.2 Caenorhabditis elegans RNA-dependent RNA polymerase Family (rrf-3), partial mRNA.

Price & Availability↑

CloneID	OCa337517
Clone ID Related Accession (Same CDS sequence)	NM_063312.5 , NM_063312.6
Accession Version	NM_063312.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	RNA-dependent RNA polymerase Family
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_063312.4. COMPLETENESS: incomplete on both ends.

ATGCTCCCGT TTGATAATGA TGACTCTAGC GATGATGCTA CAACTTCTGT TAGGCCGAAG 
CATCCAAGAG GAGTTCCTCA GAGTCAATCA ACATTTCCAC GTGGTAGATC GAATTTTTCG 
TCTGGAACTT TACCAAATCG AAAAACAGAA TGCACTCCAG TGAACACCTT AACAATTGGG 
CATTCAAACA AGATGCTATT GACAACGTTT CGGATGGATC GAAATTCAAA ATCTAAATCG 
GAAGTTGACG TACAAGAACA GCCTGTTCAC TCTTCATCGT CGGCTTTTCC AGGAAATCAT 
TTAAACAATT TTTCGTATCC AGTGAATCGT GGATATCTTC GTGATTACCT GCTACAGTCA 
CAAAGGCCTT CTACTTCAAA ACCAGTCGAC TGTTCTGTTC TAAAACGGCA CTCTCTCCCA 
TCAACTCACA TTCTTTATGA GAAAACGAAG CACAGAGGTG GTGTAAATAT CGAAGAACAA 
GAAAAACTCG TGCGGATGTT ATGGGCCGCT GCAGAAGAAT CGGAAACAGT TGCGAAGACG 
AGACAGTTTA GTAAAAAACA GGCGATTGAG CTGAATTTCG ATGCTAAGCT CATTGGCAGC 
ATGAATAATG ATTGCTTTGG ATATTGCCGA GCACACATGG AAAATATAAA AGATGTTTTG 
AAAACACACT TGAAATTGTC TAAAGTTGAC GAGGTAAACT GGATCAAGGT GGGGATGGTG 
CCACGAGCTG CGTACGAAGA TAAATCATAC GTCATCGATG CTCATTTAGT TTTGACGCCA 
AACGGTGAAG TAGAAGATGA AAATGAATTG TTCTCCGAGT TCGCATCAAG TTTCACATCT 
AGAATAACTG GAATGCTCCA TGATCAAGTA TTTTTGGAAG TCCCGAAAAT GCATACGCTT 
TTCACAAAAA TAACACCCCA ACACATGGAT ATAAATATTT CTGCGATTGC GATTGGAAAC 
TGCCCTAATT CAGGACTTTT CCTTGTTAGA GGAGATTTCA TCAGTCAAGA AAACACAGTT 
TGTAGTGTAA AGCTTCAGTC ACACCATAAT GCAGATGCAT CCCGTGAAAA CAGTTCATTC 
AAAGTGGCAG GAAGCAATAA ATACCTCTCG TATGCGAGAT TTGAGCATGA CAAAAGACTG 
GCAGTGGTAT ATTTCGGAGT TCGATTAGCC GAATTCGCTG ATGATGGTTT GGATCATGCC 
GGATTTCGTC TTAATCTATA TTATAATTTG TTTGTTCGAA TTGTTGTCGA CATGTCTCAT 
GAAACTACGA ACAGTATTTA TATACAAATG AAAAATCCAC CGCATCTCTG GGAAGGAATT 
CCGAAAAATA CGATTTTTCA CCCATCAAAG TCGAAGGTTC TCAATATGGA AACGTGCACA 
GAATGGACAA GAGTATTGAG TTGGCCTGGC GATGCTGAGG GCCGAGGTGT CGGTTGTACT 
TCTGAAGCTT TCTCGCAAAG CTCCTGGATC CGGTTGACAA TGAGAAAAGA TGATGATAAC 
GATTCGGTAT CGTCTACACA ATTGATGGAT ATTGTTACTA GACTTTCTGC TCGAAGCAAA 
GCAAAAGTGA TGTTCGGCTC CATTTTTTCA ATTCGCCGAA AACTTGCACC ATCCCCGGCT 
TTCCATTCTC TGGGATCTTT CCGAGCGAAT TATGCTCTCC AGGCTCTAAT CACACGCGGA 
AGTGTATTTA CGGACCAACT TTTCGACGCA ACTGATGAAA ATATTCCAAG TTCAGACAAT 
GACAATGATG AAGATGATGA CGATGATGTT GATGACACGA AAAAGCCAAT GGAATTAGTA 
CACGAGCCGC TCTTTCTGAA ATTAGTTCGT AGGGGAATGA AAGAATGCTC TCAAGCCACA 
GAAGAGACTC TCGAACAACT TTTGAATGCA TTTGATGAAC GTCGTCAAAT CGATGTAGTT 
ACCGCGTTCA CAACAATGTA TCAATCAAGA AAGATTCAAT ACGAAAGACT TCTGAAAGGA 
GAAAGCCTTC AAGATGTAGG CCTTGCAAAG CCATTGCCGA AGAATTGTGT TTCAGTGGCT 
AAAGTTATTG TAACACCATC TCGAATACTG CTAATGGCTC CAGAAGTAAT GATGGTAAAT 
CGAGTTGTTA GAAGATTTGG ACCTGATTAT GCTCTTCGTT GTGTGTTCCG AGATGACAAT 
CTGGGCCGGC TCGCTATTCG CGACTTTTCC ATCAATAATA TAGATCACAT GTCGAATATT 
GTCACCGAAG GAATTTATTT GACTCTCAAA AATGGAATTC AAGTTGCTGA TCGTGTCTAC 
AGTTTTCTAG GATGGAGTAA TTCTCAAATG CGTGATCAAG GATGTTATTT ATACGCTCCT 
CGAGTCAATG CATTAACTGG TGAAGTCACA GGAACTGTAG AAGATATTAG AGTATGGATG 
GGAGATTTCC GAGATGCGAT TAGTGTTCCA AAGATGATGA GTCGAATGGG ACAATGCTTT 
ACACAAGCAC AACCAACGGT ACGTCTTGAA CGACATCACT GGATCGTTGA ACCTGACATT 
GAAGGTGGTG TAGAGAACAA ATATTGTTTT TCTGATGGTT GTGGTCGAAT TTCTATCAAA 
CTGGCAACAC ATATATCTAA GATTCTCCAA TTGAAGGAAG TACCAGCTTG TTTCCAAGTG 
CGATTTAAAG GTTTCAAAGG AATTCTAGTT ATTGATCCAA CAATCGATGA TATTATAAAT 
ATGCCTAAAG TTATCTTCAG AAAAAGTCAG CAAAAGTTTG GTGAAGGTGG TGGAGAGCTG 
CAAGATGAAT ACCTCGAAGT AGTAAAATAC GCTATGCCAT CTCCAGTTTG TCTCAACCGT 
CCATTCATTA CAATCCTGGA TCAAGTGTCG GAAAAACAAT CTGCAAGCTC TCACAGAAGG 
ATCACAAATC GTGTTCATTA TTACTTGGAA AGAGAACTAT GTTCTCTGAG TAATATGCTC 
ATAAATGAAA ATCAAGCAGC AGAAGAGCTA GTCAATCGCA CAAACTTGGC AATCGACTGG 
AACGCAGCAA GCAAACGAGC CGGGTTCGAA TTGAGTGTAG ATCCATTGAT ACGAGATATG 
CTATTCTCTA TTTATCGCTA CAACATCATC CATCACATTT CTAAAGCTAA AATTTTCTTG 
CCTCCAAGTT TGGGAAGATC AATGTACGGT GTTGTCGATG AGACTGGACT TTTACAGTAC 
GGACAGGTTT TCATCCAGTA TTCACCAAGT ATCCGACAAA CCAGTAACCG TCCAATATTG 
AAAACAGGCA AAGTTTTGAT TACCAAAAAT CCGTGTCACG TTCCTGGAGA TGTTCGAGTT 
TTCGACGCAG TTTGGCAACC AGCATTAGCT CATTTAGTCG ATGTTGTAGT ATTTCCACAA 
CATGGCCCAC GACCACATCC CGATGAAATG GCAGGTTCAG ATTTGGATGG AGATGAGTAT 
TCTATAATAT GGGATCAAGA AATGCTACTC GATTATAATG AAGAAGCAAT GGTTTTTCCG 
AGTAGTTCAG CTGCAGAAGA AGACAAAGAA CCTACTACAG ATGATATGGT TGAATTCTTC 
CTTCGATACC TTCAACAGGA TTCAATTGGA AGAATGAGTC ACGCTCATTT GGCTTATGCT 
GATCTTCACG GACTTTTCCA CGAAAATTGT CACGCCATCG CGCTCAAATG TGCAGTAGCT 
GTAGATTTTC CGAAAAGTGG AGTTCCCGCG GAACCATTGT CAAGTTTCGA GCAATGCGAA 
ATGACACCTG ATTATATGAT GTCAGGTGGA AAACCGATGT ACTACAGTAC CCGATTAAAT 
GGACAATTGC ACCGAAAAGC TCGAAAAGTT GAAGAAGTTT TGGAAGAATT TGAAACACGC 
GGGAGTGTAT TCGAAAGAGA ATATGATAAA CTGATTTGTC CAGAAGACGT TGATGTTTTC 
TTCGGAAATG AAATAAAACT TGTACAAACA TTGACTCTTC GCGATGAATA CGTTGATCGA 
ATGCAACAAC TTTTGGATGA ATATGGAATT GAAGACGAAG CTTCAGTTGT GTCTGGACAT 
GCTGCAAGTA TCAAGAGATT AGCTGGAATG GAGAGAGACG ACTATTCATT TTATCACACT 
GATAAGGTCG TTGAGCTTCG TTATGAAAAA TTGTATGCTG TATTTCGTGC AAAATTCTTT 
GAAGAGTTTG GTGGAGAAGA AATCAACATT GAGAATGATG GAAAGAACAC TCGGTTGAAA 
TGTACGAAGG CAATGCATGA AAAGATTCGG CAATGGTATT TCGTGGCATA TGTGCAGCCA 
AAGATAAATA AGGCTGGAAG ATGCATTGGA CAGAGTCTTC CCTGGGTTGC TTGGGATGCA 
TTGTGTGATT TGAGGCGACA ATTAATGCTC GATAAAAATG ACGCAGTTTT ACGAGGCAAA 
TATCCAATTG CTGCTCGTTT AGAAGAGGAG ATCGAAAACT CCATTGAAAG GCAGTTTGAC 
AAGTTTCTCA AATTGAAAGA TTTGATTGAA TCTCACAAAG ATGCGCTTTT CCTGAGACGA 
TACGTATATT TTTATGGAGA TCAGATTATA AAAATGTTGT TCATCCTGAA AGTTTGGCTT 
GAACGAGAAA ATGTGCTCCC ATCAAGTGTT TTGTCGATTT GGCAGCTGGG AAGACTTTTG 
ATTCGTCTAG GGCTCGGAGA TCTCCTGGGA AATCCCACTA TTGATTATGA AAAAAGTCTT 
CTGATGCCAA CAACAATGTT TCAGCAATGG ATTTCGAAGA AAGAAGATGC AGATGAAGCT 
CCGATATTGA GAAATTTCGA TATGGGAACA ATGATGCTCG AATTTCTCAG ATATTTGGCA 
AGTCAATCTT TCGCATCAGC TGAAAGCATA AGTCTTCGAG TTTTCTACGA AAAAGATATT 
GTTGAGCCAA TACTTACTAA AAGTGCACAA TGGATGCCTT TACATCTTAT TGCATATCGT 
ACATTTCATT CCATCGCTGT GTCTGGACGG TTTGATGCGC TTCATTTGGA CGATGAGGAT 
GCGGTGGATC AGATTACAGA AAGCAAGGAT CCAATACTAG TTAACGAGTC ATTGTTCTCT 
TCGAGAAACT ACAACGATGA TTATCCGATC AGTAGGAGTA GAATATTGCA ATCTTTGAAG 
GATTGGTCTG GTGTCAAAGA AATAATTCCT CGCGAAATCA CTGGAACTCG GAAGTCTGAT 
ATGATCTATG TTACATCAGT TGGAACGGTT CTTGCACGAC AACGCCTCGC TCGACTTTTA 
CTTCTCAGTG GAGAGACAAT TAGGGACGCG ATTGCCAACA ACGTCGTGCC GAATGAAGTA 
CGCGACGAGT TCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_495713.2
CDS	1..5298
Translation	MLPFDNDDSSDDATTSVRPKHPRGVPQSQSTFPRGRSNFSSGTLPNRKTECTPVNTLTIGHSNKMLLTTFRMDRNSKSKSEVDVQEQPVHSSSSAFPGNHLNNFSYPVNRGYLRDYLLQSQRPSTSKPVDCSVLKRHSLPSTHILYEKTKHRGGVNIEEQEKLVRMLWAAAEESETVAKTRQFSKKQAIELNFDAKLIGSMNNDCFGYCRAHMENIKDVLKTHLKLSKVDEVNWIKVGMVPRAAYEDKSYVIDAHLVLTPNGEVEDENELFSEFASSFTSRITGMLHDQVFLEVPKMHTLFTKITPQHMDINISAIAIGNCPNSGLFLVRGDFISQENTVCSVKLQSHHNADASRENSSFKVAGSNKYLSYARFEHDKRLAVVYFGVRLAEFADDGLDHAGFRLNLYYNLFVRIVVDMSHETTNSIYIQMKNPPHLWEGIPKNTIFHPSKSKVLNMETCTEWTRVLSWPGDAEGRGVGCTSEAFSQSSWIRLTMRKDDDNDSVSSTQLMDIVTRLSARSKAKVMFGSIFSIRRKLAPSPAFHSLGSFRANYALQALITRGSVFTDQLFDATDENIPSSDNDNDEDDDDDVDDTKKPMELVHEPLFLKLVRRGMKECSQATEETLEQLLNAFDERRQIDVVTAFTTMYQSRKIQYERLLKGESLQDVGLAKPLPKNCVSVAKVIVTPSRILLMAPEVMMVNRVVRRFGPDYALRCVFRDDNLGRLAIRDFSINNIDHMSNIVTEGIYLTLKNGIQVADRVYSFLGWSNSQMRDQGCYLYAPRVNALTGEVTGTVEDIRVWMGDFRDAISVPKMMSRMGQCFTQAQPTVRLERHHWIVEPDIEGGVENKYCFSDGCGRISIKLATHISKILQLKEVPACFQVRFKGFKGILVIDPTIDDIINMPKVIFRKSQQKFGEGGGELQDEYLEVVKYAMPSPVCLNRPFITILDQVSEKQSASSHRRITNRVHYYLERELCSLSNMLINENQAAEELVNRTNLAIDWNAASKRAGFELSVDPLIRDMLFSIYRYNIIHHISKAKIFLPPSLGRSMYGVVDETGLLQYGQVFIQYSPSIRQTSNRPILKTGKVLITKNPCHVPGDVRVFDAVWQPALAHLVDVVVFPQHGPRPHPDEMAGSDLDGDEYSIIWDQEMLLDYNEEAMVFPSSSAAEEDKEPTTDDMVEFFLRYLQQDSIGRMSHAHLAYADLHGLFHENCHAIALKCAVAVDFPKSGVPAEPLSSFEQCEMTPDYMMSGGKPMYYSTRLNGQLHRKARKVEEVLEEFETRGSVFEREYDKLICPEDVDVFFGNEIKLVQTLTLRDEYVDRMQQLLDEYGIEDEASVVSGHAASIKRLAGMERDDYSFYHTDKVVELRYEKLYAVFRAKFFEEFGGEEINIENDGKNTRLKCTKAMHEKIRQWYFVAYVQPKINKAGRCIGQSLPWVAWDALCDLRRQLMLDKNDAVLRGKYPIAARLEEEIENSIERQFDKFLKLKDLIESHKDALFLRRYVYFYGDQIIKMLFILKVWLERENVLPSSVLSIWQLGRLLIRLGLGDLLGNPTIDYEKSLLMPTTMFQQWISKKEDADEAPILRNFDMGTMMLEFLRYLASQSFASAESISLRVFYEKDIVEPILTKSAQWMPLHLIAYRTFHSIAVSGRFDALHLDDEDAVDQITESKDPILVNESLFSSRNYNDDYPISRSRILQSLKDWSGVKEIIPREITGTRKSDMIYVTSVGTVLARQRLARLLLLSGETIRDAIANNVVPNEVRDEFL

Target ORF information:

RefSeq Version	NM_063312.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans RNA-dependent RNA polymerase Family (rrf-3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_063312.5

ORF Insert Sequence:

ATGCTCCCGT TTGATAATGA TGACTCTAGC GATGATGCTA CAACTTCTGT TAGGCCGAAG 
CATCCAAGAG GAGTTCCTCA GAGTCAATCA ACATTTCCAC GTGGTAGATC GAATTTTTCG 
TCTGGAACTT TACCAAATCG AAAAACAGAA TGCACTCCAG TGAACACCTT AACAATTGGG 
CATTCAAACA AGATGCTATT GACAACGTTT CGGATGGATC GAAATTCAAA ATCTAAATCG 
GAAGTTGACG TACAAGAACA GCCTGTTCAC TCTTCATCGT CGGCTTTTCC AGGAAATCAT 
TTAAACAATT TTTCGTATCC AGTGAATCGT GGATATCTTC GTGATTACCT GCTACAGTCA 
CAAAGGCCTT CTACTTCAAA ACCAGTCGAC TGTTCTGTTC TAAAACGGCA CTCTCTCCCA 
TCAACTCACA TTCTTTATGA GAAAACGAAG CACAGAGGTG GTGTAAATAT CGAAGAACAA 
GAAAAACTCG TGCGGATGTT ATGGGCCGCT GCAGAAGAAT CGGAAACAGT TGCGAAGACG 
AGACAGTTTA GTAAAAAACA GGCGATTGAG CTGAATTTCG ATGCTAAGCT CATTGGCAGC 
ATGAATAATG ATTGCTTTGG ATATTGCCGA GCACACATGG AAAATATAAA AGATGTTTTG 
AAAACACACT TGAAATTGTC TAAAGTTGAC GAGGTAAACT GGATCAAGGT GGGGATGGTG 
CCACGAGCTG CGTACGAAGA TAAATCATAC GTCATCGATG CTCATTTAGT TTTGACGCCA 
AACGGTGAAG TAGAAGATGA AAATGAATTG TTCTCCGAGT TCGCATCAAG TTTCACATCT 
AGAATAACTG GAATGCTCCA TGATCAAGTA TTTTTGGAAG TCCCGAAAAT GCATACGCTT 
TTCACAAAAA TAACACCCCA ACACATGGAT ATAAATATTT CTGCGATTGC GATTGGAAAC 
TGCCCTAATT CAGGACTTTT CCTTGTTAGA GGAGATTTCA TCAGTCAAGA AAACACAGTT 
TGTAGTGTAA AGCTTCAGTC ACACCATAAT GCAGATGCAT CCCGTGAAAA CAGTTCATTC 
AAAGTGGCAG GAAGCAATAA ATACCTCTCG TATGCGAGAT TTGAGCATGA CAAAAGACTG 
GCAGTGGTAT ATTTCGGAGT TCGATTAGCC GAATTCGCTG ATGATGGTTT GGATCATGCC 
GGATTTCGTC TTAATCTATA TTATAATTTG TTTGTTCGAA TTGTTGTCGA CATGTCTCAT 
GAAACTACGA ACAGTATTTA TATACAAATG AAAAATCCAC CGCATCTCTG GGAAGGAATT 
CCGAAAAATA CGATTTTTCA CCCATCAAAG TCGAAGGTTC TCAATATGGA AACGTGCACA 
GAATGGACAA GAGTATTGAG TTGGCCTGGC GATGCTGAGG GCCGAGGTGT CGGTTGTACT 
TCTGAAGCTT TCTCGCAAAG CTCCTGGATC CGGTTGACAA TGAGAAAAGA TGATGATAAC 
GATTCGGTAT CGTCTACACA ATTGATGGAT ATTGTTACTA GACTTTCTGC TCGAAGCAAA 
GCAAAAGTGA TGTTCGGCTC CATTTTTTCA ATTCGCCGAA AACTTGCACC ATCCCCGGCT 
TTCCATTCTC TGGGATCTTT CCGAGCGAAT TATGCTCTCC AGGCTCTAAT CACACGCGGA 
AGTGTATTTA CGGACCAACT TTTCGACGCA ACTGATGAAA ATATTCCAAG TTCAGACAAT 
GACAATGATG AAGATGATGA CGATGATGTT GATGACACGA AAAAGCCAAT GGAATTAGTA 
CACGAGCCGC TCTTTCTGAA ATTAGTTCGT AGGGGAATGA AAGAATGCTC TCAAGCCACA 
GAAGAGACTC TCGAACAACT TTTGAATGCA TTTGATGAAC GTCGTCAAAT CGATGTAGTT 
ACCGCGTTCA CAACAATGTA TCAATCAAGA AAGATTCAAT ACGAAAGACT TCTGAAAGGA 
GAAAGCCTTC AAGATGTAGG CCTTGCAAAG CCATTGCCGA AGAATTGTGT TTCAGTGGCT 
AAAGTTATTG TAACACCATC TCGAATACTG CTAATGGCTC CAGAAGTAAT GATGGTAAAT 
CGAGTTGTTA GAAGATTTGG ACCTGATTAT GCTCTTCGTT GTGTGTTCCG AGATGACAAT 
CTGGGCCGGC TCGCTATTCG CGACTTTTCC ATCAATAATA TAGATCACAT GTCGAATATT 
GTCACCGAAG GAATTTATTT GACTCTCAAA AATGGAATTC AAGTTGCTGA TCGTGTCTAC 
AGTTTTCTAG GATGGAGTAA TTCTCAAATG CGTGATCAAG GATGTTATTT ATACGCTCCT 
CGAGTCAATG CATTAACTGG TGAAGTCACA GGAACTGTAG AAGATATTAG AGTATGGATG 
GGAGATTTCC GAGATGCGAT TAGTGTTCCA AAGATGATGA GTCGAATGGG ACAATGCTTT 
ACACAAGCAC AACCAACGGT ACGTCTTGAA CGACATCACT GGATCGTTGA ACCTGACATT 
GAAGGTGGTG TAGAGAACAA ATATTGTTTT TCTGATGGTT GTGGTCGAAT TTCTATCAAA 
CTGGCAACAC ATATATCTAA GATTCTCCAA TTGAAGGAAG TACCAGCTTG TTTCCAAGTG 
CGATTTAAAG GTTTCAAAGG AATTCTAGTT ATTGATCCAA CAATCGATGA TATTATAAAT 
ATGCCTAAAG TTATCTTCAG AAAAAGTCAG CAAAAGTTTG GTGAAGGTGG TGGAGAGCTG 
CAAGATGAAT ACCTCGAAGT AGTAAAATAC GCTATGCCAT CTCCAGTTTG TCTCAACCGT 
CCATTCATTA CAATCCTGGA TCAAGTGTCG GAAAAACAAT CTGCAAGCTC TCACAGAAGG 
ATCACAAATC GTGTTCATTA TTACTTGGAA AGAGAACTAT GTTCTCTGAG TAATATGCTC 
ATAAATGAAA ATCAAGCAGC AGAAGAGCTA GTCAATCGCA CAAACTTGGC AATCGACTGG 
AACGCAGCAA GCAAACGAGC CGGGTTCGAA TTGAGTGTAG ATCCATTGAT ACGAGATATG 
CTATTCTCTA TTTATCGCTA CAACATCATC CATCACATTT CTAAAGCTAA AATTTTCTTG 
CCTCCAAGTT TGGGAAGATC AATGTACGGT GTTGTCGATG AGACTGGACT TTTACAGTAC 
GGACAGGTTT TCATCCAGTA TTCACCAAGT ATCCGACAAA CCAGTAACCG TCCAATATTG 
AAAACAGGCA AAGTTTTGAT TACCAAAAAT CCGTGTCACG TTCCTGGAGA TGTTCGAGTT 
TTCGACGCAG TTTGGCAACC AGCATTAGCT CATTTAGTCG ATGTTGTAGT ATTTCCACAA 
CATGGCCCAC GACCACATCC CGATGAAATG GCAGGTTCAG ATTTGGATGG AGATGAGTAT 
TCTATAATAT GGGATCAAGA AATGCTACTC GATTATAATG AAGAAGCAAT GGTTTTTCCG 
AGTAGTTCAG CTGCAGAAGA AGACAAAGAA CCTACTACAG ATGATATGGT TGAATTCTTC 
CTTCGATACC TTCAACAGGA TTCAATTGGA AGAATGAGTC ACGCTCATTT GGCTTATGCT 
GATCTTCACG GACTTTTCCA CGAAAATTGT CACGCCATCG CGCTCAAATG TGCAGTAGCT 
GTAGATTTTC CGAAAAGTGG AGTTCCCGCG GAACCATTGT CAAGTTTCGA GCAATGCGAA 
ATGACACCTG ATTATATGAT GTCAGGTGGA AAACCGATGT ACTACAGTAC CCGATTAAAT 
GGACAATTGC ACCGAAAAGC TCGAAAAGTT GAAGAAGTTT TGGAAGAATT TGAAACACGC 
GGGAGTGTAT TCGAAAGAGA ATATGATAAA CTGATTTGTC CAGAAGACGT TGATGTTTTC 
TTCGGAAATG AAATAAAACT TGTACAAACA TTGACTCTTC GCGATGAATA CGTTGATCGA 
ATGCAACAAC TTTTGGATGA ATATGGAATT GAAGACGAAG CTTCAGTTGT GTCTGGACAT 
GCTGCAAGTA TCAAGAGATT AGCTGGAATG GAGAGAGACG ACTATTCATT TTATCACACT 
GATAAGGTCG TTGAGCTTCG TTATGAAAAA TTGTATGCTG TATTTCGTGC AAAATTCTTT 
GAAGAGTTTG GTGGAGAAGA AATCAACATT GAGAATGATG GAAAGAACAC TCGGTTGAAA 
TGTACGAAGG CAATGCATGA AAAGATTCGG CAATGGTATT TCGTGGCATA TGTGCAGCCA 
AAGATAAATA AGGCTGGAAG ATGCATTGGA CAGAGTCTTC CCTGGGTTGC TTGGGATGCA 
TTGTGTGATT TGAGGCGACA ATTAATGCTC GATAAAAATG ACGCAGTTTT ACGAGGCAAA 
TATCCAATTG CTGCTCGTTT AGAAGAGGAG ATCGAAAACT CCATTGAAAG GCAGTTTGAC 
AAGTTTCTCA AATTGAAAGA TTTGATTGAA TCTCACAAAG ATGCGCTTTT CCTGAGACGA 
TACGTATATT TTTATGGAGA TCAGATTATA AAAATGTTGT TCATCCTGAA AGTTTGGCTT 
GAACGAGAAA ATGTGCTCCC ATCAAGTGTT TTGTCGATTT GGCAGCTGGG AAGACTTTTG 
ATTCGTCTAG GGCTCGGAGA TCTCCTGGGA AATCCCACTA TTGATTATGA AAAAAGTCTT 
CTGATGCCAA CAACAATGTT TCAGCAATGG ATTTCGAAGA AAGAAGATGC AGATGAAGCT 
CCGATATTGA GAAATTTCGA TATGGGAACA ATGATGCTCG AATTTCTCAG ATATTTGGCA 
AGTCAATCTT TCGCATCAGC TGAAAGCATA AGTCTTCGAG TTTTCTACGA AAAAGATATT 
GTTGAGCCAA TACTTACTAA AAGTGCACAA TGGATGCCTT TACATCTTAT TGCATATCGT 
ACATTTCATT CCATCGCTGT GTCTGGACGG TTTGATGCGC TTCATTTGGA CGATGAGGAT 
GCGGTGGATC AGATTACAGA AAGCAAGGAT CCAATACTAG TTAACGAGTC ATTGTTCTCT 
TCGAGAAACT ACAACGATGA TTATCCGATC AGTAGGAGTA GAATATTGCA ATCTTTGAAG 
GATTGGTCTG GTGTCAAAGA AATAATTCCT CGCGAAATCA CTGGAACTCG GAAGTCTGAT 
ATGATCTATG TTACATCAGT TGGAACGGTT CTTGCACGAC AACGCCTCGC TCGACTTTTA 
CTTCTCAGTG GAGAGACAAT TAGGGACGCG ATTGCCAACA ACGTCGTGCC GAATGAAGTA 
CGCGACGAGT TCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rrf-3 ( NM_063312.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_495713.2 Caenorhabditis elegans RNA-dependent RNA polymerase Family (rrf-3), mRNA.

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CloneID	OCa337517
Clone ID Related Accession (Same CDS sequence)	NM_063312.5 , NM_063312.6
Accession Version	NM_063312.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	RNA-dependent RNA polymerase Family
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_063312.5.

ATGCTCCCGT TTGATAATGA TGACTCTAGC GATGATGCTA CAACTTCTGT TAGGCCGAAG 
CATCCAAGAG GAGTTCCTCA GAGTCAATCA ACATTTCCAC GTGGTAGATC GAATTTTTCG 
TCTGGAACTT TACCAAATCG AAAAACAGAA TGCACTCCAG TGAACACCTT AACAATTGGG 
CATTCAAACA AGATGCTATT GACAACGTTT CGGATGGATC GAAATTCAAA ATCTAAATCG 
GAAGTTGACG TACAAGAACA GCCTGTTCAC TCTTCATCGT CGGCTTTTCC AGGAAATCAT 
TTAAACAATT TTTCGTATCC AGTGAATCGT GGATATCTTC GTGATTACCT GCTACAGTCA 
CAAAGGCCTT CTACTTCAAA ACCAGTCGAC TGTTCTGTTC TAAAACGGCA CTCTCTCCCA 
TCAACTCACA TTCTTTATGA GAAAACGAAG CACAGAGGTG GTGTAAATAT CGAAGAACAA 
GAAAAACTCG TGCGGATGTT ATGGGCCGCT GCAGAAGAAT CGGAAACAGT TGCGAAGACG 
AGACAGTTTA GTAAAAAACA GGCGATTGAG CTGAATTTCG ATGCTAAGCT CATTGGCAGC 
ATGAATAATG ATTGCTTTGG ATATTGCCGA GCACACATGG AAAATATAAA AGATGTTTTG 
AAAACACACT TGAAATTGTC TAAAGTTGAC GAGGTAAACT GGATCAAGGT GGGGATGGTG 
CCACGAGCTG CGTACGAAGA TAAATCATAC GTCATCGATG CTCATTTAGT TTTGACGCCA 
AACGGTGAAG TAGAAGATGA AAATGAATTG TTCTCCGAGT TCGCATCAAG TTTCACATCT 
AGAATAACTG GAATGCTCCA TGATCAAGTA TTTTTGGAAG TCCCGAAAAT GCATACGCTT 
TTCACAAAAA TAACACCCCA ACACATGGAT ATAAATATTT CTGCGATTGC GATTGGAAAC 
TGCCCTAATT CAGGACTTTT CCTTGTTAGA GGAGATTTCA TCAGTCAAGA AAACACAGTT 
TGTAGTGTAA AGCTTCAGTC ACACCATAAT GCAGATGCAT CCCGTGAAAA CAGTTCATTC 
AAAGTGGCAG GAAGCAATAA ATACCTCTCG TATGCGAGAT TTGAGCATGA CAAAAGACTG 
GCAGTGGTAT ATTTCGGAGT TCGATTAGCC GAATTCGCTG ATGATGGTTT GGATCATGCC 
GGATTTCGTC TTAATCTATA TTATAATTTG TTTGTTCGAA TTGTTGTCGA CATGTCTCAT 
GAAACTACGA ACAGTATTTA TATACAAATG AAAAATCCAC CGCATCTCTG GGAAGGAATT 
CCGAAAAATA CGATTTTTCA CCCATCAAAG TCGAAGGTTC TCAATATGGA AACGTGCACA 
GAATGGACAA GAGTATTGAG TTGGCCTGGC GATGCTGAGG GCCGAGGTGT CGGTTGTACT 
TCTGAAGCTT TCTCGCAAAG CTCCTGGATC CGGTTGACAA TGAGAAAAGA TGATGATAAC 
GATTCGGTAT CGTCTACACA ATTGATGGAT ATTGTTACTA GACTTTCTGC TCGAAGCAAA 
GCAAAAGTGA TGTTCGGCTC CATTTTTTCA ATTCGCCGAA AACTTGCACC ATCCCCGGCT 
TTCCATTCTC TGGGATCTTT CCGAGCGAAT TATGCTCTCC AGGCTCTAAT CACACGCGGA 
AGTGTATTTA CGGACCAACT TTTCGACGCA ACTGATGAAA ATATTCCAAG TTCAGACAAT 
GACAATGATG AAGATGATGA CGATGATGTT GATGACACGA AAAAGCCAAT GGAATTAGTA 
CACGAGCCGC TCTTTCTGAA ATTAGTTCGT AGGGGAATGA AAGAATGCTC TCAAGCCACA 
GAAGAGACTC TCGAACAACT TTTGAATGCA TTTGATGAAC GTCGTCAAAT CGATGTAGTT 
ACCGCGTTCA CAACAATGTA TCAATCAAGA AAGATTCAAT ACGAAAGACT TCTGAAAGGA 
GAAAGCCTTC AAGATGTAGG CCTTGCAAAG CCATTGCCGA AGAATTGTGT TTCAGTGGCT 
AAAGTTATTG TAACACCATC TCGAATACTG CTAATGGCTC CAGAAGTAAT GATGGTAAAT 
CGAGTTGTTA GAAGATTTGG ACCTGATTAT GCTCTTCGTT GTGTGTTCCG AGATGACAAT 
CTGGGCCGGC TCGCTATTCG CGACTTTTCC ATCAATAATA TAGATCACAT GTCGAATATT 
GTCACCGAAG GAATTTATTT GACTCTCAAA AATGGAATTC AAGTTGCTGA TCGTGTCTAC 
AGTTTTCTAG GATGGAGTAA TTCTCAAATG CGTGATCAAG GATGTTATTT ATACGCTCCT 
CGAGTCAATG CATTAACTGG TGAAGTCACA GGAACTGTAG AAGATATTAG AGTATGGATG 
GGAGATTTCC GAGATGCGAT TAGTGTTCCA AAGATGATGA GTCGAATGGG ACAATGCTTT 
ACACAAGCAC AACCAACGGT ACGTCTTGAA CGACATCACT GGATCGTTGA ACCTGACATT 
GAAGGTGGTG TAGAGAACAA ATATTGTTTT TCTGATGGTT GTGGTCGAAT TTCTATCAAA 
CTGGCAACAC ATATATCTAA GATTCTCCAA TTGAAGGAAG TACCAGCTTG TTTCCAAGTG 
CGATTTAAAG GTTTCAAAGG AATTCTAGTT ATTGATCCAA CAATCGATGA TATTATAAAT 
ATGCCTAAAG TTATCTTCAG AAAAAGTCAG CAAAAGTTTG GTGAAGGTGG TGGAGAGCTG 
CAAGATGAAT ACCTCGAAGT AGTAAAATAC GCTATGCCAT CTCCAGTTTG TCTCAACCGT 
CCATTCATTA CAATCCTGGA TCAAGTGTCG GAAAAACAAT CTGCAAGCTC TCACAGAAGG 
ATCACAAATC GTGTTCATTA TTACTTGGAA AGAGAACTAT GTTCTCTGAG TAATATGCTC 
ATAAATGAAA ATCAAGCAGC AGAAGAGCTA GTCAATCGCA CAAACTTGGC AATCGACTGG 
AACGCAGCAA GCAAACGAGC CGGGTTCGAA TTGAGTGTAG ATCCATTGAT ACGAGATATG 
CTATTCTCTA TTTATCGCTA CAACATCATC CATCACATTT CTAAAGCTAA AATTTTCTTG 
CCTCCAAGTT TGGGAAGATC AATGTACGGT GTTGTCGATG AGACTGGACT TTTACAGTAC 
GGACAGGTTT TCATCCAGTA TTCACCAAGT ATCCGACAAA CCAGTAACCG TCCAATATTG 
AAAACAGGCA AAGTTTTGAT TACCAAAAAT CCGTGTCACG TTCCTGGAGA TGTTCGAGTT 
TTCGACGCAG TTTGGCAACC AGCATTAGCT CATTTAGTCG ATGTTGTAGT ATTTCCACAA 
CATGGCCCAC GACCACATCC CGATGAAATG GCAGGTTCAG ATTTGGATGG AGATGAGTAT 
TCTATAATAT GGGATCAAGA AATGCTACTC GATTATAATG AAGAAGCAAT GGTTTTTCCG 
AGTAGTTCAG CTGCAGAAGA AGACAAAGAA CCTACTACAG ATGATATGGT TGAATTCTTC 
CTTCGATACC TTCAACAGGA TTCAATTGGA AGAATGAGTC ACGCTCATTT GGCTTATGCT 
GATCTTCACG GACTTTTCCA CGAAAATTGT CACGCCATCG CGCTCAAATG TGCAGTAGCT 
GTAGATTTTC CGAAAAGTGG AGTTCCCGCG GAACCATTGT CAAGTTTCGA GCAATGCGAA 
ATGACACCTG ATTATATGAT GTCAGGTGGA AAACCGATGT ACTACAGTAC CCGATTAAAT 
GGACAATTGC ACCGAAAAGC TCGAAAAGTT GAAGAAGTTT TGGAAGAATT TGAAACACGC 
GGGAGTGTAT TCGAAAGAGA ATATGATAAA CTGATTTGTC CAGAAGACGT TGATGTTTTC 
TTCGGAAATG AAATAAAACT TGTACAAACA TTGACTCTTC GCGATGAATA CGTTGATCGA 
ATGCAACAAC TTTTGGATGA ATATGGAATT GAAGACGAAG CTTCAGTTGT GTCTGGACAT 
GCTGCAAGTA TCAAGAGATT AGCTGGAATG GAGAGAGACG ACTATTCATT TTATCACACT 
GATAAGGTCG TTGAGCTTCG TTATGAAAAA TTGTATGCTG TATTTCGTGC AAAATTCTTT 
GAAGAGTTTG GTGGAGAAGA AATCAACATT GAGAATGATG GAAAGAACAC TCGGTTGAAA 
TGTACGAAGG CAATGCATGA AAAGATTCGG CAATGGTATT TCGTGGCATA TGTGCAGCCA 
AAGATAAATA AGGCTGGAAG ATGCATTGGA CAGAGTCTTC CCTGGGTTGC TTGGGATGCA 
TTGTGTGATT TGAGGCGACA ATTAATGCTC GATAAAAATG ACGCAGTTTT ACGAGGCAAA 
TATCCAATTG CTGCTCGTTT AGAAGAGGAG ATCGAAAACT CCATTGAAAG GCAGTTTGAC 
AAGTTTCTCA AATTGAAAGA TTTGATTGAA TCTCACAAAG ATGCGCTTTT CCTGAGACGA 
TACGTATATT TTTATGGAGA TCAGATTATA AAAATGTTGT TCATCCTGAA AGTTTGGCTT 
GAACGAGAAA ATGTGCTCCC ATCAAGTGTT TTGTCGATTT GGCAGCTGGG AAGACTTTTG 
ATTCGTCTAG GGCTCGGAGA TCTCCTGGGA AATCCCACTA TTGATTATGA AAAAAGTCTT 
CTGATGCCAA CAACAATGTT TCAGCAATGG ATTTCGAAGA AAGAAGATGC AGATGAAGCT 
CCGATATTGA GAAATTTCGA TATGGGAACA ATGATGCTCG AATTTCTCAG ATATTTGGCA 
AGTCAATCTT TCGCATCAGC TGAAAGCATA AGTCTTCGAG TTTTCTACGA AAAAGATATT 
GTTGAGCCAA TACTTACTAA AAGTGCACAA TGGATGCCTT TACATCTTAT TGCATATCGT 
ACATTTCATT CCATCGCTGT GTCTGGACGG TTTGATGCGC TTCATTTGGA CGATGAGGAT 
GCGGTGGATC AGATTACAGA AAGCAAGGAT CCAATACTAG TTAACGAGTC ATTGTTCTCT 
TCGAGAAACT ACAACGATGA TTATCCGATC AGTAGGAGTA GAATATTGCA ATCTTTGAAG 
GATTGGTCTG GTGTCAAAGA AATAATTCCT CGCGAAATCA CTGGAACTCG GAAGTCTGAT 
ATGATCTATG TTACATCAGT TGGAACGGTT CTTGCACGAC AACGCCTCGC TCGACTTTTA 
CTTCTCAGTG GAGAGACAAT TAGGGACGCG ATTGCCAACA ACGTCGTGCC GAATGAAGTA 
CGCGACGAGT TCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_495713.2
CDS	94..5391
Translation	MLPFDNDDSSDDATTSVRPKHPRGVPQSQSTFPRGRSNFSSGTLPNRKTECTPVNTLTIGHSNKMLLTTFRMDRNSKSKSEVDVQEQPVHSSSSAFPGNHLNNFSYPVNRGYLRDYLLQSQRPSTSKPVDCSVLKRHSLPSTHILYEKTKHRGGVNIEEQEKLVRMLWAAAEESETVAKTRQFSKKQAIELNFDAKLIGSMNNDCFGYCRAHMENIKDVLKTHLKLSKVDEVNWIKVGMVPRAAYEDKSYVIDAHLVLTPNGEVEDENELFSEFASSFTSRITGMLHDQVFLEVPKMHTLFTKITPQHMDINISAIAIGNCPNSGLFLVRGDFISQENTVCSVKLQSHHNADASRENSSFKVAGSNKYLSYARFEHDKRLAVVYFGVRLAEFADDGLDHAGFRLNLYYNLFVRIVVDMSHETTNSIYIQMKNPPHLWEGIPKNTIFHPSKSKVLNMETCTEWTRVLSWPGDAEGRGVGCTSEAFSQSSWIRLTMRKDDDNDSVSSTQLMDIVTRLSARSKAKVMFGSIFSIRRKLAPSPAFHSLGSFRANYALQALITRGSVFTDQLFDATDENIPSSDNDNDEDDDDDVDDTKKPMELVHEPLFLKLVRRGMKECSQATEETLEQLLNAFDERRQIDVVTAFTTMYQSRKIQYERLLKGESLQDVGLAKPLPKNCVSVAKVIVTPSRILLMAPEVMMVNRVVRRFGPDYALRCVFRDDNLGRLAIRDFSINNIDHMSNIVTEGIYLTLKNGIQVADRVYSFLGWSNSQMRDQGCYLYAPRVNALTGEVTGTVEDIRVWMGDFRDAISVPKMMSRMGQCFTQAQPTVRLERHHWIVEPDIEGGVENKYCFSDGCGRISIKLATHISKILQLKEVPACFQVRFKGFKGILVIDPTIDDIINMPKVIFRKSQQKFGEGGGELQDEYLEVVKYAMPSPVCLNRPFITILDQVSEKQSASSHRRITNRVHYYLERELCSLSNMLINENQAAEELVNRTNLAIDWNAASKRAGFELSVDPLIRDMLFSIYRYNIIHHISKAKIFLPPSLGRSMYGVVDETGLLQYGQVFIQYSPSIRQTSNRPILKTGKVLITKNPCHVPGDVRVFDAVWQPALAHLVDVVVFPQHGPRPHPDEMAGSDLDGDEYSIIWDQEMLLDYNEEAMVFPSSSAAEEDKEPTTDDMVEFFLRYLQQDSIGRMSHAHLAYADLHGLFHENCHAIALKCAVAVDFPKSGVPAEPLSSFEQCEMTPDYMMSGGKPMYYSTRLNGQLHRKARKVEEVLEEFETRGSVFEREYDKLICPEDVDVFFGNEIKLVQTLTLRDEYVDRMQQLLDEYGIEDEASVVSGHAASIKRLAGMERDDYSFYHTDKVVELRYEKLYAVFRAKFFEEFGGEEINIENDGKNTRLKCTKAMHEKIRQWYFVAYVQPKINKAGRCIGQSLPWVAWDALCDLRRQLMLDKNDAVLRGKYPIAARLEEEIENSIERQFDKFLKLKDLIESHKDALFLRRYVYFYGDQIIKMLFILKVWLERENVLPSSVLSIWQLGRLLIRLGLGDLLGNPTIDYEKSLLMPTTMFQQWISKKEDADEAPILRNFDMGTMMLEFLRYLASQSFASAESISLRVFYEKDIVEPILTKSAQWMPLHLIAYRTFHSIAVSGRFDALHLDDEDAVDQITESKDPILVNESLFSSRNYNDDYPISRSRILQSLKDWSGVKEIIPREITGTRKSDMIYVTSVGTVLARQRLARLLLLSGETIRDAIANNVVPNEVRDEFL

Target ORF information:

RefSeq Version	NM_063312.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans RNA-dependent RNA polymerase Family (rrf-3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_063312.6

ORF Insert Sequence:

ATGCTCCCGT TTGATAATGA TGACTCTAGC GATGATGCTA CAACTTCTGT TAGGCCGAAG 
CATCCAAGAG GAGTTCCTCA GAGTCAATCA ACATTTCCAC GTGGTAGATC GAATTTTTCG 
TCTGGAACTT TACCAAATCG AAAAACAGAA TGCACTCCAG TGAACACCTT AACAATTGGG 
CATTCAAACA AGATGCTATT GACAACGTTT CGGATGGATC GAAATTCAAA ATCTAAATCG 
GAAGTTGACG TACAAGAACA GCCTGTTCAC TCTTCATCGT CGGCTTTTCC AGGAAATCAT 
TTAAACAATT TTTCGTATCC AGTGAATCGT GGATATCTTC GTGATTACCT GCTACAGTCA 
CAAAGGCCTT CTACTTCAAA ACCAGTCGAC TGTTCTGTTC TAAAACGGCA CTCTCTCCCA 
TCAACTCACA TTCTTTATGA GAAAACGAAG CACAGAGGTG GTGTAAATAT CGAAGAACAA 
GAAAAACTCG TGCGGATGTT ATGGGCCGCT GCAGAAGAAT CGGAAACAGT TGCGAAGACG 
AGACAGTTTA GTAAAAAACA GGCGATTGAG CTGAATTTCG ATGCTAAGCT CATTGGCAGC 
ATGAATAATG ATTGCTTTGG ATATTGCCGA GCACACATGG AAAATATAAA AGATGTTTTG 
AAAACACACT TGAAATTGTC TAAAGTTGAC GAGGTAAACT GGATCAAGGT GGGGATGGTG 
CCACGAGCTG CGTACGAAGA TAAATCATAC GTCATCGATG CTCATTTAGT TTTGACGCCA 
AACGGTGAAG TAGAAGATGA AAATGAATTG TTCTCCGAGT TCGCATCAAG TTTCACATCT 
AGAATAACTG GAATGCTCCA TGATCAAGTA TTTTTGGAAG TCCCGAAAAT GCATACGCTT 
TTCACAAAAA TAACACCCCA ACACATGGAT ATAAATATTT CTGCGATTGC GATTGGAAAC 
TGCCCTAATT CAGGACTTTT CCTTGTTAGA GGAGATTTCA TCAGTCAAGA AAACACAGTT 
TGTAGTGTAA AGCTTCAGTC ACACCATAAT GCAGATGCAT CCCGTGAAAA CAGTTCATTC 
AAAGTGGCAG GAAGCAATAA ATACCTCTCG TATGCGAGAT TTGAGCATGA CAAAAGACTG 
GCAGTGGTAT ATTTCGGAGT TCGATTAGCC GAATTCGCTG ATGATGGTTT GGATCATGCC 
GGATTTCGTC TTAATCTATA TTATAATTTG TTTGTTCGAA TTGTTGTCGA CATGTCTCAT 
GAAACTACGA ACAGTATTTA TATACAAATG AAAAATCCAC CGCATCTCTG GGAAGGAATT 
CCGAAAAATA CGATTTTTCA CCCATCAAAG TCGAAGGTTC TCAATATGGA AACGTGCACA 
GAATGGACAA GAGTATTGAG TTGGCCTGGC GATGCTGAGG GCCGAGGTGT CGGTTGTACT 
TCTGAAGCTT TCTCGCAAAG CTCCTGGATC CGGTTGACAA TGAGAAAAGA TGATGATAAC 
GATTCGGTAT CGTCTACACA ATTGATGGAT ATTGTTACTA GACTTTCTGC TCGAAGCAAA 
GCAAAAGTGA TGTTCGGCTC CATTTTTTCA ATTCGCCGAA AACTTGCACC ATCCCCGGCT 
TTCCATTCTC TGGGATCTTT CCGAGCGAAT TATGCTCTCC AGGCTCTAAT CACACGCGGA 
AGTGTATTTA CGGACCAACT TTTCGACGCA ACTGATGAAA ATATTCCAAG TTCAGACAAT 
GACAATGATG AAGATGATGA CGATGATGTT GATGACACGA AAAAGCCAAT GGAATTAGTA 
CACGAGCCGC TCTTTCTGAA ATTAGTTCGT AGGGGAATGA AAGAATGCTC TCAAGCCACA 
GAAGAGACTC TCGAACAACT TTTGAATGCA TTTGATGAAC GTCGTCAAAT CGATGTAGTT 
ACCGCGTTCA CAACAATGTA TCAATCAAGA AAGATTCAAT ACGAAAGACT TCTGAAAGGA 
GAAAGCCTTC AAGATGTAGG CCTTGCAAAG CCATTGCCGA AGAATTGTGT TTCAGTGGCT 
AAAGTTATTG TAACACCATC TCGAATACTG CTAATGGCTC CAGAAGTAAT GATGGTAAAT 
CGAGTTGTTA GAAGATTTGG ACCTGATTAT GCTCTTCGTT GTGTGTTCCG AGATGACAAT 
CTGGGCCGGC TCGCTATTCG CGACTTTTCC ATCAATAATA TAGATCACAT GTCGAATATT 
GTCACCGAAG GAATTTATTT GACTCTCAAA AATGGAATTC AAGTTGCTGA TCGTGTCTAC 
AGTTTTCTAG GATGGAGTAA TTCTCAAATG CGTGATCAAG GATGTTATTT ATACGCTCCT 
CGAGTCAATG CATTAACTGG TGAAGTCACA GGAACTGTAG AAGATATTAG AGTATGGATG 
GGAGATTTCC GAGATGCGAT TAGTGTTCCA AAGATGATGA GTCGAATGGG ACAATGCTTT 
ACACAAGCAC AACCAACGGT ACGTCTTGAA CGACATCACT GGATCGTTGA ACCTGACATT 
GAAGGTGGTG TAGAGAACAA ATATTGTTTT TCTGATGGTT GTGGTCGAAT TTCTATCAAA 
CTGGCAACAC ATATATCTAA GATTCTCCAA TTGAAGGAAG TACCAGCTTG TTTCCAAGTG 
CGATTTAAAG GTTTCAAAGG AATTCTAGTT ATTGATCCAA CAATCGATGA TATTATAAAT 
ATGCCTAAAG TTATCTTCAG AAAAAGTCAG CAAAAGTTTG GTGAAGGTGG TGGAGAGCTG 
CAAGATGAAT ACCTCGAAGT AGTAAAATAC GCTATGCCAT CTCCAGTTTG TCTCAACCGT 
CCATTCATTA CAATCCTGGA TCAAGTGTCG GAAAAACAAT CTGCAAGCTC TCACAGAAGG 
ATCACAAATC GTGTTCATTA TTACTTGGAA AGAGAACTAT GTTCTCTGAG TAATATGCTC 
ATAAATGAAA ATCAAGCAGC AGAAGAGCTA GTCAATCGCA CAAACTTGGC AATCGACTGG 
AACGCAGCAA GCAAACGAGC CGGGTTCGAA TTGAGTGTAG ATCCATTGAT ACGAGATATG 
CTATTCTCTA TTTATCGCTA CAACATCATC CATCACATTT CTAAAGCTAA AATTTTCTTG 
CCTCCAAGTT TGGGAAGATC AATGTACGGT GTTGTCGATG AGACTGGACT TTTACAGTAC 
GGACAGGTTT TCATCCAGTA TTCACCAAGT ATCCGACAAA CCAGTAACCG TCCAATATTG 
AAAACAGGCA AAGTTTTGAT TACCAAAAAT CCGTGTCACG TTCCTGGAGA TGTTCGAGTT 
TTCGACGCAG TTTGGCAACC AGCATTAGCT CATTTAGTCG ATGTTGTAGT ATTTCCACAA 
CATGGCCCAC GACCACATCC CGATGAAATG GCAGGTTCAG ATTTGGATGG AGATGAGTAT 
TCTATAATAT GGGATCAAGA AATGCTACTC GATTATAATG AAGAAGCAAT GGTTTTTCCG 
AGTAGTTCAG CTGCAGAAGA AGACAAAGAA CCTACTACAG ATGATATGGT TGAATTCTTC 
CTTCGATACC TTCAACAGGA TTCAATTGGA AGAATGAGTC ACGCTCATTT GGCTTATGCT 
GATCTTCACG GACTTTTCCA CGAAAATTGT CACGCCATCG CGCTCAAATG TGCAGTAGCT 
GTAGATTTTC CGAAAAGTGG AGTTCCCGCG GAACCATTGT CAAGTTTCGA GCAATGCGAA 
ATGACACCTG ATTATATGAT GTCAGGTGGA AAACCGATGT ACTACAGTAC CCGATTAAAT 
GGACAATTGC ACCGAAAAGC TCGAAAAGTT GAAGAAGTTT TGGAAGAATT TGAAACACGC 
GGGAGTGTAT TCGAAAGAGA ATATGATAAA CTGATTTGTC CAGAAGACGT TGATGTTTTC 
TTCGGAAATG AAATAAAACT TGTACAAACA TTGACTCTTC GCGATGAATA CGTTGATCGA 
ATGCAACAAC TTTTGGATGA ATATGGAATT GAAGACGAAG CTTCAGTTGT GTCTGGACAT 
GCTGCAAGTA TCAAGAGATT AGCTGGAATG GAGAGAGACG ACTATTCATT TTATCACACT 
GATAAGGTCG TTGAGCTTCG TTATGAAAAA TTGTATGCTG TATTTCGTGC AAAATTCTTT 
GAAGAGTTTG GTGGAGAAGA AATCAACATT GAGAATGATG GAAAGAACAC TCGGTTGAAA 
TGTACGAAGG CAATGCATGA AAAGATTCGG CAATGGTATT TCGTGGCATA TGTGCAGCCA 
AAGATAAATA AGGCTGGAAG ATGCATTGGA CAGAGTCTTC CCTGGGTTGC TTGGGATGCA 
TTGTGTGATT TGAGGCGACA ATTAATGCTC GATAAAAATG ACGCAGTTTT ACGAGGCAAA 
TATCCAATTG CTGCTCGTTT AGAAGAGGAG ATCGAAAACT CCATTGAAAG GCAGTTTGAC 
AAGTTTCTCA AATTGAAAGA TTTGATTGAA TCTCACAAAG ATGCGCTTTT CCTGAGACGA 
TACGTATATT TTTATGGAGA TCAGATTATA AAAATGTTGT TCATCCTGAA AGTTTGGCTT 
GAACGAGAAA ATGTGCTCCC ATCAAGTGTT TTGTCGATTT GGCAGCTGGG AAGACTTTTG 
ATTCGTCTAG GGCTCGGAGA TCTCCTGGGA AATCCCACTA TTGATTATGA AAAAAGTCTT 
CTGATGCCAA CAACAATGTT TCAGCAATGG ATTTCGAAGA AAGAAGATGC AGATGAAGCT 
CCGATATTGA GAAATTTCGA TATGGGAACA ATGATGCTCG AATTTCTCAG ATATTTGGCA 
AGTCAATCTT TCGCATCAGC TGAAAGCATA AGTCTTCGAG TTTTCTACGA AAAAGATATT 
GTTGAGCCAA TACTTACTAA AAGTGCACAA TGGATGCCTT TACATCTTAT TGCATATCGT 
ACATTTCATT CCATCGCTGT GTCTGGACGG TTTGATGCGC TTCATTTGGA CGATGAGGAT 
GCGGTGGATC AGATTACAGA AAGCAAGGAT CCAATACTAG TTAACGAGTC ATTGTTCTCT 
TCGAGAAACT ACAACGATGA TTATCCGATC AGTAGGAGTA GAATATTGCA ATCTTTGAAG 
GATTGGTCTG GTGTCAAAGA AATAATTCCT CGCGAAATCA CTGGAACTCG GAAGTCTGAT 
ATGATCTATG TTACATCAGT TGGAACGGTT CTTGCACGAC AACGCCTCGC TCGACTTTTA 
CTTCTCAGTG GAGAGACAAT TAGGGACGCG ATTGCCAACA ACGTCGTGCC GAATGAAGTA 
CGCGACGAGT TCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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rrf-3 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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