cki-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	cki-1
Entrez Gene ID	174260
Full Name	Cyclin-dependent kinase inhibitor 1
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_063240.5	NP_495641.1	Cyclin-dependent kinase inhibitor 1
NM_063240.4	NP_495641.1	Cyclin-dependent kinase inhibitor 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data also suggest that integrin plays a major role in maintaining proper CKI-1/p27(KIP1) levels in the cell.
Title: A novel mutation in ? integrin reveals an integrin-mediated interaction between the extracellular matrix and cki-1/p27KIP1.

BRAP-2 is necessary for preventing an inappropriate response to elevated levels of reactive oxygen species by countering premature activation of BRC-1 and CKI-1.
Title: Developmental arrest of Caenorhabditis elegans BRAP-2 mutant exposed to oxidative stress is dependent on BRC-1.

Both cki-1 and cki-2 mediate cell cycle quiescence, but our genetic and phenotypic analyses demonstrate that they act within distinct pathways to exert control over the cell cycle machinery.
Title: The cyclin-dependent kinase inhibitors, cki-1 and cki-2, act in overlapping but distinct pathways to control cell cycle quiescence during C. elegans development.

cki-1 is required to ensure the proper formation ans specification of the gonadal sheath cell precursors.
Title: cki-1 links cell division and cell fate acquisition in the C. elegans somatic gonad.

CKI-1 cyclin-dependent kinase inhibitor has an essential embryonic role in cell cycle exit and morphogenesis.
Title: Essential embryonic roles of the CKI-1 cyclin-dependent kinase inhibitor in cell-cycle exit and morphogenesis in C elegans.

The following cki-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cki-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa339765	NM_063240.5 Latest version!	Caenorhabditis elegans Cyclin-dependent kinase inhibitor 1 (cki-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OCa339765	NM_063240.4	Caenorhabditis elegans Cyclin-dependent kinase inhibitor 1 (cki-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cki-1 ( NM_063240.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_495641.1 Caenorhabditis elegans Cyclin-dependent kinase inhibitor 1 (cki-1), mRNA.

Price & Availability↑

CloneID	OCa339765
Clone ID Related Accession (Same CDS sequence)	NM_063240.5 , NM_063240.4
Accession Version	NM_063240.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Cyclin-dependent kinase inhibitor 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_063240.4.

ATGTCTTCTG CTCGTCGTTG CCTTTTCGGT CGTCCGACGC CCGAGCAACG CTCCAGGACT 
CGAATTTGGC TTGAAGATGC TGTTAAGCGC ATGCGCCAGG AAGAAAGCCA GAAATGGGGA 
TTCGACTTTG AACTGGAGAC TCCCCTCCCA AGCTCTGCTG GATTCGTTTA TGAAGTTATT 
CCAGAGAATT GTGTTCCGGA GTTCTACAGA ACCAAAGTTC TCACTGTCAG AACCACATGC 
TCATCGCTGG ACATCAGCTC AACGACTTTG ACTCCATTGA GCTCTCCGAG CACATCTGAT 
AAGGAGGAGC CCTCGCTGAT GGATCCCAAC AGCTCGTTCG AAGATGAAGA GGAACCGAAG 
AAGTGGCAAT TCAGAGAGCC ACCAACTCCA CGGAAGACCC CAACAAAGCG TCAGCAGAAG 
ATGACCGACT TCATGGCAGT TTCCCGTAAG AAGAATTCGT TGTCTCCAAA CAAGCTGTCT 
CCGGTGAATG TGATCTTCAC TCCAAAATCT CGTCGTCCAA CGATCAGAAC TCGATCTTCA 
TGCTCTCCAT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_495641.1
CDS	20..574
Translation	MSSARRCLFGRPTPEQRSRTRIWLEDAVKRMRQEESQKWGFDFELETPLPSSAGFVYEVIPENCVPEFYRTKVLTVRTTCSSLDISSTTLTPLSSPSTSDKEEPSLMDPNSSFEDEEEPKKWQFREPPTPRKTPTKRQQKMTDFMAVSRKKNSLSPNKLSPVNVIFTPKSRRPTIRTRSSCSPY

Target ORF information:

RefSeq Version	NM_063240.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Cyclin-dependent kinase inhibitor 1 (cki-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_063240.5

ORF Insert Sequence:

ATGTCTTCTG CTCGTCGTTG CCTTTTCGGT CGTCCGACGC CCGAGCAACG CTCCAGGACT 
CGAATTTGGC TTGAAGATGC TGTTAAGCGC ATGCGCCAGG AAGAAAGCCA GAAATGGGGA 
TTCGACTTTG AACTGGAGAC TCCCCTCCCA AGCTCTGCTG GATTCGTTTA TGAAGTTATT 
CCAGAGAATT GTGTTCCGGA GTTCTACAGA ACCAAAGTTC TCACTGTCAG AACCACATGC 
TCATCGCTGG ACATCAGCTC AACGACTTTG ACTCCATTGA GCTCTCCGAG CACATCTGAT 
AAGGAGGAGC CCTCGCTGAT GGATCCCAAC AGCTCGTTCG AAGATGAAGA GGAACCGAAG 
AAGTGGCAAT TCAGAGAGCC ACCAACTCCA CGGAAGACCC CAACAAAGCG TCAGCAGAAG 
ATGACCGACT TCATGGCAGT TTCCCGTAAG AAGAATTCGT TGTCTCCAAA CAAGCTGTCT 
CCGGTGAATG TGATCTTCAC TCCAAAATCT CGTCGTCCAA CGATCAGAAC TCGATCTTCA 
TGCTCTCCAT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cki-1 ( NM_063240.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_495641.1 Caenorhabditis elegans Cyclin-dependent kinase inhibitor 1 (cki-1), partial mRNA.

Price & Availability↑

CloneID	OCa339765
Clone ID Related Accession (Same CDS sequence)	NM_063240.5 , NM_063240.4
Accession Version	NM_063240.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Cyclin-dependent kinase inhibitor 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_063240.3. COMPLETENESS: incomplete on both ends.

ATGTCTTCTG CTCGTCGTTG CCTTTTCGGT CGTCCGACGC CCGAGCAACG CTCCAGGACT 
CGAATTTGGC TTGAAGATGC TGTTAAGCGC ATGCGCCAGG AAGAAAGCCA GAAATGGGGA 
TTCGACTTTG AACTGGAGAC TCCCCTCCCA AGCTCTGCTG GATTCGTTTA TGAAGTTATT 
CCAGAGAATT GTGTTCCGGA GTTCTACAGA ACCAAAGTTC TCACTGTCAG AACCACATGC 
TCATCGCTGG ACATCAGCTC AACGACTTTG ACTCCATTGA GCTCTCCGAG CACATCTGAT 
AAGGAGGAGC CCTCGCTGAT GGATCCCAAC AGCTCGTTCG AAGATGAAGA GGAACCGAAG 
AAGTGGCAAT TCAGAGAGCC ACCAACTCCA CGGAAGACCC CAACAAAGCG TCAGCAGAAG 
ATGACCGACT TCATGGCAGT TTCCCGTAAG AAGAATTCGT TGTCTCCAAA CAAGCTGTCT 
CCGGTGAATG TGATCTTCAC TCCAAAATCT CGTCGTCCAA CGATCAGAAC TCGATCTTCA 
TGCTCTCCAT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_495641.1
CDS	1..555
Translation	MSSARRCLFGRPTPEQRSRTRIWLEDAVKRMRQEESQKWGFDFELETPLPSSAGFVYEVIPENCVPEFYRTKVLTVRTTCSSLDISSTTLTPLSSPSTSDKEEPSLMDPNSSFEDEEEPKKWQFREPPTPRKTPTKRQQKMTDFMAVSRKKNSLSPNKLSPVNVIFTPKSRRPTIRTRSSCSPY

Target ORF information:

RefSeq Version	NM_063240.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Cyclin-dependent kinase inhibitor 1 (cki-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_063240.4

ORF Insert Sequence:

ATGTCTTCTG CTCGTCGTTG CCTTTTCGGT CGTCCGACGC CCGAGCAACG CTCCAGGACT 
CGAATTTGGC TTGAAGATGC TGTTAAGCGC ATGCGCCAGG AAGAAAGCCA GAAATGGGGA 
TTCGACTTTG AACTGGAGAC TCCCCTCCCA AGCTCTGCTG GATTCGTTTA TGAAGTTATT 
CCAGAGAATT GTGTTCCGGA GTTCTACAGA ACCAAAGTTC TCACTGTCAG AACCACATGC 
TCATCGCTGG ACATCAGCTC AACGACTTTG ACTCCATTGA GCTCTCCGAG CACATCTGAT 
AAGGAGGAGC CCTCGCTGAT GGATCCCAAC AGCTCGTTCG AAGATGAAGA GGAACCGAAG 
AAGTGGCAAT TCAGAGAGCC ACCAACTCCA CGGAAGACCC CAACAAAGCG TCAGCAGAAG 
ATGACCGACT TCATGGCAGT TTCCCGTAAG AAGAATTCGT TGTCTCCAAA CAAGCTGTCT 
CCGGTGAATG TGATCTTCAC TCCAAAATCT CGTCGTCCAA CGATCAGAAC TCGATCTTCA 
TGCTCTCCAT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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cki-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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