che-10 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	che-10
Entrez Gene ID	173801
Full Name	Rootletin
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_062419.5	NP_494820.3	Rootletin
NM_182228.5	NP_872028.2	Rootletin
NM_062420.4	NP_494821.3	Rootletin
NM_062420.3	NP_494821.3	Rootletin
NM_062419.6	NP_494820.3	Rootletin
NM_182228.4	NP_872028.2	Rootletin
NM_062418.7	NP_494819.3	Rootletin
NM_062418.6	NP_494819.3	Rootletin

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

C. elegans Girdin also regulates localization of the apical junction component AJM-1, suggesting that in nematodes Girdin may position BBs via rootletin- and AJM-1-dependent anchoring to the cytoskeleton and plasma membrane, respectively. Together, our results describe a conserved role for Girdin in BB positioning and ciliogenesis
Title: A Conserved Role for Girdin in Basal Body Positioning and Ciliogenesis.

The following che-10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the che-10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa339201	NM_182228.5 Latest version!	Caenorhabditis elegans Rootletin (che-10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCa339202	NM_062420.4 Latest version!	Caenorhabditis elegans Rootletin (che-10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCa339203	NM_062418.7 Latest version!	Caenorhabditis elegans Rootletin (che-10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCa339204	NM_062419.6 Latest version!	Caenorhabditis elegans Rootletin (che-10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OCa339202	NM_062420.3	Caenorhabditis elegans Rootletin (che-10), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa339204	NM_062419.5	Caenorhabditis elegans Rootletin (che-10), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa339201	NM_182228.4	Caenorhabditis elegans Rootletin (che-10), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCa339203	NM_062418.6	Caenorhabditis elegans Rootletin (che-10), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

che-10 ( NM_182228.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_872028.2 Caenorhabditis elegans Rootletin (che-10), mRNA.

Price & Availability↑

CloneID	OCa339201
Clone ID Related Accession (Same CDS sequence)	NM_182228.5 , NM_182228.4
Accession Version	NM_182228.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Rootletin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_182228.4.

ATGGATGTGG CACGTAAAAT GCGGAAGCTG CTGACCACCA AGAACGGGGA AATCGATGAA 
TCAAGGGAAG CAGCGAAGCA GGCAGAAAAG GAAAGAGATC GTGCAAAGAA GGATTTGGAA 
AAAGAAGAGA AACGAAGAAA AGATGACAGA GAAGCAGAGA GAAAACGATC AAGTGTCTAT 
TCACAGAGAG AACATGACCT GAAAAAGTTG GATGATGAGT TGAGGAAGGC CAGTGAAAAG 
ATCCGGAATC TCGAGGAGCA ACGGGAGAGC CAGGAAAAAC TCACCATATC AGTTCAGAAC 
TCATTGAATG AAGCACACAG GCAACACAAA CAGTTTATTG AGGAATTGAT GATTCGGCAT 
CGAGAAGAAC TGAAAGAGCG GGAGGATTCT CACGAGGAAG CGTTACGTTC CAAAGATACC 
GAGGAAAGAT CTCGATTTGA AAAAGAACGT TCTGAACGGG AAAAGATTAG ACGAGAAAGT 
GATGAGCTCA GAGAGACGCA GAGAAGTTTA AAGGGTGACG TGGCGGCGAT GAAGACAGAT 
TTGGATGATA AGACTTTAAG GCTTGACATG CTCGAAACAG AACGGGATGA GCTCAAAAAG 
AAACTGGAAA CCGAAAGAGA GCAGGCTGAT CAGAGAGACT TGGAGATAGC AGAGTGTCGA 
GCAAAGCTTG ATGAGATGGC TGAGAAGGAA GCGGAGCTCC GGAAAGAGCT CGCAGAGTTC 
CAGGCAATTA TCACTGCGAT GGAAGGAGAA GGAAAGCTGA ATCAAGAACA GTTTTTGGAG 
AGTAAGAATG AGTTGAACAC GCTGACAGAT CAAATTGAAT CTTTGAATAG TGAGGTGGAG 
AATAAGAATG AGGAAATTCG AAATTTGATG GCTACACTTC AAGAAAAAGA AGTTCACATT 
CAAAACGTCC GAACATCAAG TCATCAGTTG ACTGCCACAT ACGAAGAGGC TAACGGGGAA 
ATCGATATTT TGAAGGCTGA ACTTACCCGG CTCCACGAGC AAGTCAATGA GAGGACTCGA 
CAGATCTCCG AAGCTAATGA GAAGTATGAT GATGCAGCTC GGAAGAATGA CGCATTGCTC 
GAAGATGTCG CCACGTGGCA AGAGAAGTAT GAGCAGTTGA AGATGGAGCT GGAAGAAATG 
AACAGAAGAG GACAGGAGAA GGAACGAGAA GAAGCAGATC TTCGAGCATT ACTTGATGAT 
TTACGTGGAA ACTTTGATAA GTTGACTAAT GAGCTGAAGC AGAAGGGTGT TACTGTTGAT 
AGCTTGAATG AAGAAATTTC ATCGTTGAAA GAACAATTAA ACAAGTCAGA AAAGGAGAGA 
AAAGAAGAAC TGTTAAGGAT GGAAGAGCTT GAACAGAAAA ATGAAGCGGA AATGAAAGAA 
GAGTATGAAG TAAAGTTACA ATTGGCTGAG AAGGATCGTC AAGGAGTTGA GAATTTTGGA 
AAAGAATGTG AAGCGAGAAT GAATGAGCTC ACGAAGATCC ACGAGATGCT AATGGAAGAG 
CACGATCAGT TGAAAGTGGA TCATCTGCAC ACGGAAGAAG AAGTGGAGAG GTTGAAAGAG 
AAAATGAGAA AGGAATTGGA GAAGTTGAAT GAGCAGAATG ATGGTGATCG GGCGGAATGG 
AGTAATGAAA GGAATCGGCT GGAATCATCC AAAAACGAAG CAGTTACGGA ACTTCAAGAA 
CGAGTGCAGA AGCTTGAGGA TGTGGTGAAG GAGAAAGAAG ATAAGGAAAT TGCTCTTCGT 
AGGGATTTAG AAGACAGCCA TGAGAAATCA AGAGATCTTG ATGACAAGCT TCGAAAAATG 
GAGTTGACAG ATGAGGAGAA AGAGGAAGAT CGGAAGAAGG AACAGAAGAC ATTGAATGAA 
GAGAGGATGA AACTCATGGA ACAGAAAGAA GAAGCAATGC TTGTTGCTAC AAAACATGCA 
ACAACGATTG ATCAACAGAC TCGAAGAATT TCAGTGTTGG AGGGTGACGT GGAAAAGCTG 
ACGGCTGGAA TTGCGGAGAG AGAAAGTTCG ATTAATGCTT TGGAATCGAA TACAATGGAG 
CTGATATCCA AGCTGGAAAC TACTGAAGCA GAACTTGAAA AGCTGAAAGA CGAGCTTGCA 
GTCATGCTGA AACAAAACTC GGAACTCAAA AATGGAAAAG AGGGGTTGAG TGAGAAGTGG 
AATGAGGAGA GGAAGAAGAT CCAAGACTTG GCAGATCAAT TGAGAGAGGC TAACAAAGTT 
GTTCATAATA TGAGAATGAA GAATGTTAAT CTAGAAGAAA AGAAGAATGA ATTGGATCAG 
AATGTGACTG ATTTGACCAA TAAAGTTCGA CAATTAGAGA TTCAGTTGAT GGATAAAGCT 
GCGAAGAACG AGGTTTCAGG AGATTTGCTC AGAAAGATGG AGCATGATGC TCAGAGTATG 
CTGAAGCAAG CACAGAATGA GCAGTTTAGA CTAACTGATC TTGAAAAAGT TCGAAAAGCT 
CTTCAAGATG AGAATCAACG GCTTGTAAAT GATCTGGCAA CAGTGAAAGC TGCATTTGAA 
GTCAAGAGAG AAACGAGCAA ATCTGCAATC AGTGACATTT TGGATAAGTA CCGGAGTGCT 
GAGGAGAAGG CCAATAAGGG TGAATTGGAT AATCAGAGGC TTAGAAGTGA TCTGGCTACT 
GTGACATTAA AACTGGAACG TCAAGAACTG AAAGCAAAAG ATTCTGATAA TCGACTGAGA 
GATAGCCAAA AACGATTCGA AGAAGTTCAA TCAAAACTTG CAAATCTTCA GAAATCAGCA 
GTAGAATCCT TACAAAATCC TATGTCTTCG AATTCCCGCC AAAATCGGTC AATCTATGTT 
GACATTCCAC GTGCAGCTTC TTCGATCGGC CTCAATGAGA ATTCTGATGA AGTCCCGCTT 
CGCAGTTCCC CCTCAGTCCG TTTTGCAGAC AGCTCACAGA ATATGCAAAG AGCAGTTGAC 
TCTATGGATG TGAGTTCTTC AGTTGGAGTT ACACTTCGGT TTTTGAAAGA AAGAATCGAA 
CAGTTGGAAG CAGACAATGC AGATTTATCA GATGCTCTGG AAAAGGCAAA AGATGAGCTG 
AGGCAGAGAA ATGAGAAGCT GGCGGATAGG CAAATGGTTA TTGAGAGAGT TGAGAGACAA 
TTGGTTCATA TTACTGAAGA ACGCAATACA ATTGAAAATC GAATGACAAG TCAGCGTCAA 
ATGTATCTCA CAAATGAGGA ATCATCACGA TCAAGAGAAC ATGAAATCAG AAGTATGAAA 
GCCAGAATTT CAACTTTAGA GCTTCATCTT CGAGAAAAAG AATCTAAACT TGCACATCTA 
CGAAAAGAAA TTGAAGTACT TCATGGTCAG CTTCATGATG CTCTCGAATC AAAGGAAAAG 
GCTACTGGAT TGGTTGGAGT TCAAGATTCA AAGCATCGAG ACTTAGAAGA ACAACTCGAT 
CGAGCAAATC GTGAACGAGA ATTGGCAATT GGGAAGCAGA GAAGAACTCT TGCAGAGAAC 
GAGAATCTGT TCCGGAAGCT TGAACAATTG GAAAAGGAAA GAGAGCAGCT TATGCGAGAG 
ATCACAGATG AACGGAGGCT CAACGAGCGG AATCGCACAA GTCTTGAAGA ACTTCGAGTC 
TCTGAAAGAA CATGGAAATC AGCCATGACA ACTGCCAAAA AACCAGCTGA GGAGCAGGAA 
CGGGCGGTGC AGGAGCAACG GAGATGGGAA GAGTCAAATC ATGAAATGAC GAATAGAAAT 
ACTGCATTGA CAAAAGAATG TGATAGATTA CGAGTTGAAA TGAGAGATCA ACTCAACAGA 
ATGAACGGGA TCAATTTGAG ATCAGTAGAT TTCGAGAGAA AGAATGAAGA GCTTTCAAGT 
AAACTGATTG TTATGCAAAA TACAGTAACT GCGATGAAAA AGTTCGAGGA AGAATGGAAG 
AGGTTGGAGG CGGAGATGAG AGCAGAACTT AAGATTTTGA GAAAAGAAAA GCTCATGCAA 
ACTGCTGAAA TTGAGGATTT AAAGAGGAAA AGCTTCAGAA GTGACACTGA AAAGAAGGAG 
ATCGAGGGTA TTCGAGTAAG GTTGGAAAGG GAAATCAGTG CACTTAAGAG ACATGTTGAT 
GCGCTAGAAG AAGAGAAGGG AAAAACTGAA AAGGCCGTTC GAGAAACCAT GAACGAACGT 
CGTGCCATCG ACAAATCTCT CGCGTCAATG GAACGAGAAA ATCAACAGTT GTATAGGAAT 
TGTGCTCAAC TTCAAGCTCA AATTCAGAAT CTGGAGCGCG ACGCCGGAAA TCGGTCGGTA 
ACCAAGTTGG CTAAGGAGCA TTCATTGCTC GAAGCTCGAA TTGCAGCTTT AATTGAAGAA 
AAACGTCAGC TCCAATCAAT GCTTGACCAA AAAGACGCCA ACTACTCTCA CAAGCGAAAA 
CTCTTAGAAT CCCAAATCCA GTTGCTCCGC GAGCAACTCG AAGCCGAACG CCGAAAGCGT 
ACAAAGGGAG TTGTGGCGAC TGGTCCCACC GTTAGCCGAC GGGGCGTTCA GCACACATCT 
GCCTTTAGGC ACACCATCGA GCGTCATCGG TCTCTGTCCC AGTCTAGTGA ACGGACAATT 
CTTCAGGAAC GATACCTTGA ATATGTATAT ACAGGAGATC GTACTCCAGC AATACAAATG 
ATCAACACAC CGCCAATCTC TCCATTATCA CATTCTGGCT CTTTCAATTC GGACGGAAGG 
GAAGCCAGAA TCCGGAGAGA GTCTGACAAC GGCCATCTTC AGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_872028.2
CDS	12..4739
Translation	MDVARKMRKLLTTKNGEIDESREAAKQAEKERDRAKKDLEKEEKRRKDDREAERKRSSVYSQREHDLKKLDDELRKASEKIRNLEEQRESQEKLTISVQNSLNEAHRQHKQFIEELMIRHREELKEREDSHEEALRSKDTEERSRFEKERSEREKIRRESDELRETQRSLKGDVAAMKTDLDDKTLRLDMLETERDELKKKLETEREQADQRDLEIAECRAKLDEMAEKEAELRKELAEFQAIITAMEGEGKLNQEQFLESKNELNTLTDQIESLNSEVENKNEEIRNLMATLQEKEVHIQNVRTSSHQLTATYEEANGEIDILKAELTRLHEQVNERTRQISEANEKYDDAARKNDALLEDVATWQEKYEQLKMELEEMNRRGQEKEREEADLRALLDDLRGNFDKLTNELKQKGVTVDSLNEEISSLKEQLNKSEKERKEELLRMEELEQKNEAEMKEEYEVKLQLAEKDRQGVENFGKECEARMNELTKIHEMLMEEHDQLKVDHLHTEEEVERLKEKMRKELEKLNEQNDGDRAEWSNERNRLESSKNEAVTELQERVQKLEDVVKEKEDKEIALRRDLEDSHEKSRDLDDKLRKMELTDEEKEEDRKKEQKTLNEERMKLMEQKEEAMLVATKHATTIDQQTRRISVLEGDVEKLTAGIAERESSINALESNTMELISKLETTEAELEKLKDELAVMLKQNSELKNGKEGLSEKWNEERKKIQDLADQLREANKVVHNMRMKNVNLEEKKNELDQNVTDLTNKVRQLEIQLMDKAAKNEVSGDLLRKMEHDAQSMLKQAQNEQFRLTDLEKVRKALQDENQRLVNDLATVKAAFEVKRETSKSAISDILDKYRSAEEKANKGELDNQRLRSDLATVTLKLERQELKAKDSDNRLRDSQKRFEEVQSKLANLQKSAVESLQNPMSSNSRQNRSIYVDIPRAASSIGLNENSDEVPLRSSPSVRFADSSQNMQRAVDSMDVSSSVGVTLRFLKERIEQLEADNADLSDALEKAKDELRQRNEKLADRQMVIERVERQLVHITEERNTIENRMTSQRQMYLTNEESSRSREHEIRSMKARISTLELHLREKESKLAHLRKEIEVLHGQLHDALESKEKATGLVGVQDSKHRDLEEQLDRANRERELAIGKQRRTLAENENLFRKLEQLEKEREQLMREITDERRLNERNRTSLEELRVSERTWKSAMTTAKKPAEEQERAVQEQRRWEESNHEMTNRNTALTKECDRLRVEMRDQLNRMNGINLRSVDFERKNEELSSKLIVMQNTVTAMKKFEEEWKRLEAEMRAELKILRKEKLMQTAEIEDLKRKSFRSDTEKKEIEGIRVRLEREISALKRHVDALEEEKGKTEKAVRETMNERRAIDKSLASMERENQQLYRNCAQLQAQIQNLERDAGNRSVTKLAKEHSLLEARIAALIEEKRQLQSMLDQKDANYSHKRKLLESQIQLLREQLEAERRKRTKGVVATGPTVSRRGVQHTSAFRHTIERHRSLSQSSERTILQERYLEYVYTGDRTPAIQMINTPPISPLSHSGSFNSDGREARIRRESDNGHLQH

Target ORF information:

RefSeq Version	NM_182228.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Rootletin (che-10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182228.5

ORF Insert Sequence:

ATGGATGTGG CACGTAAAAT GCGGAAGCTG CTGACCACCA AGAACGGGGA AATCGATGAA 
TCAAGGGAAG CAGCGAAGCA GGCAGAAAAG GAAAGAGATC GTGCAAAGAA GGATTTGGAA 
AAAGAAGAGA AACGAAGAAA AGATGACAGA GAAGCAGAGA GAAAACGATC AAGTGTCTAT 
TCACAGAGAG AACATGACCT GAAAAAGTTG GATGATGAGT TGAGGAAGGC CAGTGAAAAG 
ATCCGGAATC TCGAGGAGCA ACGGGAGAGC CAGGAAAAAC TCACCATATC AGTTCAGAAC 
TCATTGAATG AAGCACACAG GCAACACAAA CAGTTTATTG AGGAATTGAT GATTCGGCAT 
CGAGAAGAAC TGAAAGAGCG GGAGGATTCT CACGAGGAAG CGTTACGTTC CAAAGATACC 
GAGGAAAGAT CTCGATTTGA AAAAGAACGT TCTGAACGGG AAAAGATTAG ACGAGAAAGT 
GATGAGCTCA GAGAGACGCA GAGAAGTTTA AAGGGTGACG TGGCGGCGAT GAAGACAGAT 
TTGGATGATA AGACTTTAAG GCTTGACATG CTCGAAACAG AACGGGATGA GCTCAAAAAG 
AAACTGGAAA CCGAAAGAGA GCAGGCTGAT CAGAGAGACT TGGAGATAGC AGAGTGTCGA 
GCAAAGCTTG ATGAGATGGC TGAGAAGGAA GCGGAGCTCC GGAAAGAGCT CGCAGAGTTC 
CAGGCAATTA TCACTGCGAT GGAAGGAGAA GGAAAGCTGA ATCAAGAACA GTTTTTGGAG 
AGTAAGAATG AGTTGAACAC GCTGACAGAT CAAATTGAAT CTTTGAATAG TGAGGTGGAG 
AATAAGAATG AGGAAATTCG AAATTTGATG GCTACACTTC AAGAAAAAGA AGTTCACATT 
CAAAACGTCC GAACATCAAG TCATCAGTTG ACTGCCACAT ACGAAGAGGC TAACGGGGAA 
ATCGATATTT TGAAGGCTGA ACTTACCCGG CTCCACGAGC AAGTCAATGA GAGGACTCGA 
CAGATCTCCG AAGCTAATGA GAAGTATGAT GATGCAGCTC GGAAGAATGA CGCATTGCTC 
GAAGATGTCG CCACGTGGCA AGAGAAGTAT GAGCAGTTGA AGATGGAGCT GGAAGAAATG 
AACAGAAGAG GACAGGAGAA GGAACGAGAA GAAGCAGATC TTCGAGCATT ACTTGATGAT 
TTACGTGGAA ACTTTGATAA GTTGACTAAT GAGCTGAAGC AGAAGGGTGT TACTGTTGAT 
AGCTTGAATG AAGAAATTTC ATCGTTGAAA GAACAATTAA ACAAGTCAGA AAAGGAGAGA 
AAAGAAGAAC TGTTAAGGAT GGAAGAGCTT GAACAGAAAA ATGAAGCGGA AATGAAAGAA 
GAGTATGAAG TAAAGTTACA ATTGGCTGAG AAGGATCGTC AAGGAGTTGA GAATTTTGGA 
AAAGAATGTG AAGCGAGAAT GAATGAGCTC ACGAAGATCC ACGAGATGCT AATGGAAGAG 
CACGATCAGT TGAAAGTGGA TCATCTGCAC ACGGAAGAAG AAGTGGAGAG GTTGAAAGAG 
AAAATGAGAA AGGAATTGGA GAAGTTGAAT GAGCAGAATG ATGGTGATCG GGCGGAATGG 
AGTAATGAAA GGAATCGGCT GGAATCATCC AAAAACGAAG CAGTTACGGA ACTTCAAGAA 
CGAGTGCAGA AGCTTGAGGA TGTGGTGAAG GAGAAAGAAG ATAAGGAAAT TGCTCTTCGT 
AGGGATTTAG AAGACAGCCA TGAGAAATCA AGAGATCTTG ATGACAAGCT TCGAAAAATG 
GAGTTGACAG ATGAGGAGAA AGAGGAAGAT CGGAAGAAGG AACAGAAGAC ATTGAATGAA 
GAGAGGATGA AACTCATGGA ACAGAAAGAA GAAGCAATGC TTGTTGCTAC AAAACATGCA 
ACAACGATTG ATCAACAGAC TCGAAGAATT TCAGTGTTGG AGGGTGACGT GGAAAAGCTG 
ACGGCTGGAA TTGCGGAGAG AGAAAGTTCG ATTAATGCTT TGGAATCGAA TACAATGGAG 
CTGATATCCA AGCTGGAAAC TACTGAAGCA GAACTTGAAA AGCTGAAAGA CGAGCTTGCA 
GTCATGCTGA AACAAAACTC GGAACTCAAA AATGGAAAAG AGGGGTTGAG TGAGAAGTGG 
AATGAGGAGA GGAAGAAGAT CCAAGACTTG GCAGATCAAT TGAGAGAGGC TAACAAAGTT 
GTTCATAATA TGAGAATGAA GAATGTTAAT CTAGAAGAAA AGAAGAATGA ATTGGATCAG 
AATGTGACTG ATTTGACCAA TAAAGTTCGA CAATTAGAGA TTCAGTTGAT GGATAAAGCT 
GCGAAGAACG AGGTTTCAGG AGATTTGCTC AGAAAGATGG AGCATGATGC TCAGAGTATG 
CTGAAGCAAG CACAGAATGA GCAGTTTAGA CTAACTGATC TTGAAAAAGT TCGAAAAGCT 
CTTCAAGATG AGAATCAACG GCTTGTAAAT GATCTGGCAA CAGTGAAAGC TGCATTTGAA 
GTCAAGAGAG AAACGAGCAA ATCTGCAATC AGTGACATTT TGGATAAGTA CCGGAGTGCT 
GAGGAGAAGG CCAATAAGGG TGAATTGGAT AATCAGAGGC TTAGAAGTGA TCTGGCTACT 
GTGACATTAA AACTGGAACG TCAAGAACTG AAAGCAAAAG ATTCTGATAA TCGACTGAGA 
GATAGCCAAA AACGATTCGA AGAAGTTCAA TCAAAACTTG CAAATCTTCA GAAATCAGCA 
GTAGAATCCT TACAAAATCC TATGTCTTCG AATTCCCGCC AAAATCGGTC AATCTATGTT 
GACATTCCAC GTGCAGCTTC TTCGATCGGC CTCAATGAGA ATTCTGATGA AGTCCCGCTT 
CGCAGTTCCC CCTCAGTCCG TTTTGCAGAC AGCTCACAGA ATATGCAAAG AGCAGTTGAC 
TCTATGGATG TGAGTTCTTC AGTTGGAGTT ACACTTCGGT TTTTGAAAGA AAGAATCGAA 
CAGTTGGAAG CAGACAATGC AGATTTATCA GATGCTCTGG AAAAGGCAAA AGATGAGCTG 
AGGCAGAGAA ATGAGAAGCT GGCGGATAGG CAAATGGTTA TTGAGAGAGT TGAGAGACAA 
TTGGTTCATA TTACTGAAGA ACGCAATACA ATTGAAAATC GAATGACAAG TCAGCGTCAA 
ATGTATCTCA CAAATGAGGA ATCATCACGA TCAAGAGAAC ATGAAATCAG AAGTATGAAA 
GCCAGAATTT CAACTTTAGA GCTTCATCTT CGAGAAAAAG AATCTAAACT TGCACATCTA 
CGAAAAGAAA TTGAAGTACT TCATGGTCAG CTTCATGATG CTCTCGAATC AAAGGAAAAG 
GCTACTGGAT TGGTTGGAGT TCAAGATTCA AAGCATCGAG ACTTAGAAGA ACAACTCGAT 
CGAGCAAATC GTGAACGAGA ATTGGCAATT GGGAAGCAGA GAAGAACTCT TGCAGAGAAC 
GAGAATCTGT TCCGGAAGCT TGAACAATTG GAAAAGGAAA GAGAGCAGCT TATGCGAGAG 
ATCACAGATG AACGGAGGCT CAACGAGCGG AATCGCACAA GTCTTGAAGA ACTTCGAGTC 
TCTGAAAGAA CATGGAAATC AGCCATGACA ACTGCCAAAA AACCAGCTGA GGAGCAGGAA 
CGGGCGGTGC AGGAGCAACG GAGATGGGAA GAGTCAAATC ATGAAATGAC GAATAGAAAT 
ACTGCATTGA CAAAAGAATG TGATAGATTA CGAGTTGAAA TGAGAGATCA ACTCAACAGA 
ATGAACGGGA TCAATTTGAG ATCAGTAGAT TTCGAGAGAA AGAATGAAGA GCTTTCAAGT 
AAACTGATTG TTATGCAAAA TACAGTAACT GCGATGAAAA AGTTCGAGGA AGAATGGAAG 
AGGTTGGAGG CGGAGATGAG AGCAGAACTT AAGATTTTGA GAAAAGAAAA GCTCATGCAA 
ACTGCTGAAA TTGAGGATTT AAAGAGGAAA AGCTTCAGAA GTGACACTGA AAAGAAGGAG 
ATCGAGGGTA TTCGAGTAAG GTTGGAAAGG GAAATCAGTG CACTTAAGAG ACATGTTGAT 
GCGCTAGAAG AAGAGAAGGG AAAAACTGAA AAGGCCGTTC GAGAAACCAT GAACGAACGT 
CGTGCCATCG ACAAATCTCT CGCGTCAATG GAACGAGAAA ATCAACAGTT GTATAGGAAT 
TGTGCTCAAC TTCAAGCTCA AATTCAGAAT CTGGAGCGCG ACGCCGGAAA TCGGTCGGTA 
ACCAAGTTGG CTAAGGAGCA TTCATTGCTC GAAGCTCGAA TTGCAGCTTT AATTGAAGAA 
AAACGTCAGC TCCAATCAAT GCTTGACCAA AAAGACGCCA ACTACTCTCA CAAGCGAAAA 
CTCTTAGAAT CCCAAATCCA GTTGCTCCGC GAGCAACTCG AAGCCGAACG CCGAAAGCGT 
ACAAAGGGAG TTGTGGCGAC TGGTCCCACC GTTAGCCGAC GGGGCGTTCA GCACACATCT 
GCCTTTAGGC ACACCATCGA GCGTCATCGG TCTCTGTCCC AGTCTAGTGA ACGGACAATT 
CTTCAGGAAC GATACCTTGA ATATGTATAT ACAGGAGATC GTACTCCAGC AATACAAATG 
ATCAACACAC CGCCAATCTC TCCATTATCA CATTCTGGCT CTTTCAATTC GGACGGAAGG 
GAAGCCAGAA TCCGGAGAGA GTCTGACAAC GGCCATCTTC AGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

che-10 ( NM_182228.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_872028.2 Caenorhabditis elegans Rootletin (che-10), partial mRNA.

Price & Availability↑

CloneID	OCa339201
Clone ID Related Accession (Same CDS sequence)	NM_182228.5 , NM_182228.4
Accession Version	NM_182228.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Rootletin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_182228.3. COMPLETENESS: incomplete on both ends.

ATGGATGTGG CACGTAAAAT GCGGAAGCTG CTGACCACCA AGAACGGGGA AATCGATGAA 
TCAAGGGAAG CAGCGAAGCA GGCAGAAAAG GAAAGAGATC GTGCAAAGAA GGATTTGGAA 
AAAGAAGAGA AACGAAGAAA AGATGACAGA GAAGCAGAGA GAAAACGATC AAGTGTCTAT 
TCACAGAGAG AACATGACCT GAAAAAGTTG GATGATGAGT TGAGGAAGGC CAGTGAAAAG 
ATCCGGAATC TCGAGGAGCA ACGGGAGAGC CAGGAAAAAC TCACCATATC AGTTCAGAAC 
TCATTGAATG AAGCACACAG GCAACACAAA CAGTTTATTG AGGAATTGAT GATTCGGCAT 
CGAGAAGAAC TGAAAGAGCG GGAGGATTCT CACGAGGAAG CGTTACGTTC CAAAGATACC 
GAGGAAAGAT CTCGATTTGA AAAAGAACGT TCTGAACGGG AAAAGATTAG ACGAGAAAGT 
GATGAGCTCA GAGAGACGCA GAGAAGTTTA AAGGGTGACG TGGCGGCGAT GAAGACAGAT 
TTGGATGATA AGACTTTAAG GCTTGACATG CTCGAAACAG AACGGGATGA GCTCAAAAAG 
AAACTGGAAA CCGAAAGAGA GCAGGCTGAT CAGAGAGACT TGGAGATAGC AGAGTGTCGA 
GCAAAGCTTG ATGAGATGGC TGAGAAGGAA GCGGAGCTCC GGAAAGAGCT CGCAGAGTTC 
CAGGCAATTA TCACTGCGAT GGAAGGAGAA GGAAAGCTGA ATCAAGAACA GTTTTTGGAG 
AGTAAGAATG AGTTGAACAC GCTGACAGAT CAAATTGAAT CTTTGAATAG TGAGGTGGAG 
AATAAGAATG AGGAAATTCG AAATTTGATG GCTACACTTC AAGAAAAAGA AGTTCACATT 
CAAAACGTCC GAACATCAAG TCATCAGTTG ACTGCCACAT ACGAAGAGGC TAACGGGGAA 
ATCGATATTT TGAAGGCTGA ACTTACCCGG CTCCACGAGC AAGTCAATGA GAGGACTCGA 
CAGATCTCCG AAGCTAATGA GAAGTATGAT GATGCAGCTC GGAAGAATGA CGCATTGCTC 
GAAGATGTCG CCACGTGGCA AGAGAAGTAT GAGCAGTTGA AGATGGAGCT GGAAGAAATG 
AACAGAAGAG GACAGGAGAA GGAACGAGAA GAAGCAGATC TTCGAGCATT ACTTGATGAT 
TTACGTGGAA ACTTTGATAA GTTGACTAAT GAGCTGAAGC AGAAGGGTGT TACTGTTGAT 
AGCTTGAATG AAGAAATTTC ATCGTTGAAA GAACAATTAA ACAAGTCAGA AAAGGAGAGA 
AAAGAAGAAC TGTTAAGGAT GGAAGAGCTT GAACAGAAAA ATGAAGCGGA AATGAAAGAA 
GAGTATGAAG TAAAGTTACA ATTGGCTGAG AAGGATCGTC AAGGAGTTGA GAATTTTGGA 
AAAGAATGTG AAGCGAGAAT GAATGAGCTC ACGAAGATCC ACGAGATGCT AATGGAAGAG 
CACGATCAGT TGAAAGTGGA TCATCTGCAC ACGGAAGAAG AAGTGGAGAG GTTGAAAGAG 
AAAATGAGAA AGGAATTGGA GAAGTTGAAT GAGCAGAATG ATGGTGATCG GGCGGAATGG 
AGTAATGAAA GGAATCGGCT GGAATCATCC AAAAACGAAG CAGTTACGGA ACTTCAAGAA 
CGAGTGCAGA AGCTTGAGGA TGTGGTGAAG GAGAAAGAAG ATAAGGAAAT TGCTCTTCGT 
AGGGATTTAG AAGACAGCCA TGAGAAATCA AGAGATCTTG ATGACAAGCT TCGAAAAATG 
GAGTTGACAG ATGAGGAGAA AGAGGAAGAT CGGAAGAAGG AACAGAAGAC ATTGAATGAA 
GAGAGGATGA AACTCATGGA ACAGAAAGAA GAAGCAATGC TTGTTGCTAC AAAACATGCA 
ACAACGATTG ATCAACAGAC TCGAAGAATT TCAGTGTTGG AGGGTGACGT GGAAAAGCTG 
ACGGCTGGAA TTGCGGAGAG AGAAAGTTCG ATTAATGCTT TGGAATCGAA TACAATGGAG 
CTGATATCCA AGCTGGAAAC TACTGAAGCA GAACTTGAAA AGCTGAAAGA CGAGCTTGCA 
GTCATGCTGA AACAAAACTC GGAACTCAAA AATGGAAAAG AGGGGTTGAG TGAGAAGTGG 
AATGAGGAGA GGAAGAAGAT CCAAGACTTG GCAGATCAAT TGAGAGAGGC TAACAAAGTT 
GTTCATAATA TGAGAATGAA GAATGTTAAT CTAGAAGAAA AGAAGAATGA ATTGGATCAG 
AATGTGACTG ATTTGACCAA TAAAGTTCGA CAATTAGAGA TTCAGTTGAT GGATAAAGCT 
GCGAAGAACG AGGTTTCAGG AGATTTGCTC AGAAAGATGG AGCATGATGC TCAGAGTATG 
CTGAAGCAAG CACAGAATGA GCAGTTTAGA CTAACTGATC TTGAAAAAGT TCGAAAAGCT 
CTTCAAGATG AGAATCAACG GCTTGTAAAT GATCTGGCAA CAGTGAAAGC TGCATTTGAA 
GTCAAGAGAG AAACGAGCAA ATCTGCAATC AGTGACATTT TGGATAAGTA CCGGAGTGCT 
GAGGAGAAGG CCAATAAGGG TGAATTGGAT AATCAGAGGC TTAGAAGTGA TCTGGCTACT 
GTGACATTAA AACTGGAACG TCAAGAACTG AAAGCAAAAG ATTCTGATAA TCGACTGAGA 
GATAGCCAAA AACGATTCGA AGAAGTTCAA TCAAAACTTG CAAATCTTCA GAAATCAGCA 
GTAGAATCCT TACAAAATCC TATGTCTTCG AATTCCCGCC AAAATCGGTC AATCTATGTT 
GACATTCCAC GTGCAGCTTC TTCGATCGGC CTCAATGAGA ATTCTGATGA AGTCCCGCTT 
CGCAGTTCCC CCTCAGTCCG TTTTGCAGAC AGCTCACAGA ATATGCAAAG AGCAGTTGAC 
TCTATGGATG TGAGTTCTTC AGTTGGAGTT ACACTTCGGT TTTTGAAAGA AAGAATCGAA 
CAGTTGGAAG CAGACAATGC AGATTTATCA GATGCTCTGG AAAAGGCAAA AGATGAGCTG 
AGGCAGAGAA ATGAGAAGCT GGCGGATAGG CAAATGGTTA TTGAGAGAGT TGAGAGACAA 
TTGGTTCATA TTACTGAAGA ACGCAATACA ATTGAAAATC GAATGACAAG TCAGCGTCAA 
ATGTATCTCA CAAATGAGGA ATCATCACGA TCAAGAGAAC ATGAAATCAG AAGTATGAAA 
GCCAGAATTT CAACTTTAGA GCTTCATCTT CGAGAAAAAG AATCTAAACT TGCACATCTA 
CGAAAAGAAA TTGAAGTACT TCATGGTCAG CTTCATGATG CTCTCGAATC AAAGGAAAAG 
GCTACTGGAT TGGTTGGAGT TCAAGATTCA AAGCATCGAG ACTTAGAAGA ACAACTCGAT 
CGAGCAAATC GTGAACGAGA ATTGGCAATT GGGAAGCAGA GAAGAACTCT TGCAGAGAAC 
GAGAATCTGT TCCGGAAGCT TGAACAATTG GAAAAGGAAA GAGAGCAGCT TATGCGAGAG 
ATCACAGATG AACGGAGGCT CAACGAGCGG AATCGCACAA GTCTTGAAGA ACTTCGAGTC 
TCTGAAAGAA CATGGAAATC AGCCATGACA ACTGCCAAAA AACCAGCTGA GGAGCAGGAA 
CGGGCGGTGC AGGAGCAACG GAGATGGGAA GAGTCAAATC ATGAAATGAC GAATAGAAAT 
ACTGCATTGA CAAAAGAATG TGATAGATTA CGAGTTGAAA TGAGAGATCA ACTCAACAGA 
ATGAACGGGA TCAATTTGAG ATCAGTAGAT TTCGAGAGAA AGAATGAAGA GCTTTCAAGT 
AAACTGATTG TTATGCAAAA TACAGTAACT GCGATGAAAA AGTTCGAGGA AGAATGGAAG 
AGGTTGGAGG CGGAGATGAG AGCAGAACTT AAGATTTTGA GAAAAGAAAA GCTCATGCAA 
ACTGCTGAAA TTGAGGATTT AAAGAGGAAA AGCTTCAGAA GTGACACTGA AAAGAAGGAG 
ATCGAGGGTA TTCGAGTAAG GTTGGAAAGG GAAATCAGTG CACTTAAGAG ACATGTTGAT 
GCGCTAGAAG AAGAGAAGGG AAAAACTGAA AAGGCCGTTC GAGAAACCAT GAACGAACGT 
CGTGCCATCG ACAAATCTCT CGCGTCAATG GAACGAGAAA ATCAACAGTT GTATAGGAAT 
TGTGCTCAAC TTCAAGCTCA AATTCAGAAT CTGGAGCGCG ACGCCGGAAA TCGGTCGGTA 
ACCAAGTTGG CTAAGGAGCA TTCATTGCTC GAAGCTCGAA TTGCAGCTTT AATTGAAGAA 
AAACGTCAGC TCCAATCAAT GCTTGACCAA AAAGACGCCA ACTACTCTCA CAAGCGAAAA 
CTCTTAGAAT CCCAAATCCA GTTGCTCCGC GAGCAACTCG AAGCCGAACG CCGAAAGCGT 
ACAAAGGGAG TTGTGGCGAC TGGTCCCACC GTTAGCCGAC GGGGCGTTCA GCACACATCT 
GCCTTTAGGC ACACCATCGA GCGTCATCGG TCTCTGTCCC AGTCTAGTGA ACGGACAATT 
CTTCAGGAAC GATACCTTGA ATATGTATAT ACAGGAGATC GTACTCCAGC AATACAAATG 
ATCAACACAC CGCCAATCTC TCCATTATCA CATTCTGGCT CTTTCAATTC GGACGGAAGG 
GAAGCCAGAA TCCGGAGAGA GTCTGACAAC GGCCATCTTC AGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_872028.2
CDS	1..4728
Translation	MDVARKMRKLLTTKNGEIDESREAAKQAEKERDRAKKDLEKEEKRRKDDREAERKRSSVYSQREHDLKKLDDELRKASEKIRNLEEQRESQEKLTISVQNSLNEAHRQHKQFIEELMIRHREELKEREDSHEEALRSKDTEERSRFEKERSEREKIRRESDELRETQRSLKGDVAAMKTDLDDKTLRLDMLETERDELKKKLETEREQADQRDLEIAECRAKLDEMAEKEAELRKELAEFQAIITAMEGEGKLNQEQFLESKNELNTLTDQIESLNSEVENKNEEIRNLMATLQEKEVHIQNVRTSSHQLTATYEEANGEIDILKAELTRLHEQVNERTRQISEANEKYDDAARKNDALLEDVATWQEKYEQLKMELEEMNRRGQEKEREEADLRALLDDLRGNFDKLTNELKQKGVTVDSLNEEISSLKEQLNKSEKERKEELLRMEELEQKNEAEMKEEYEVKLQLAEKDRQGVENFGKECEARMNELTKIHEMLMEEHDQLKVDHLHTEEEVERLKEKMRKELEKLNEQNDGDRAEWSNERNRLESSKNEAVTELQERVQKLEDVVKEKEDKEIALRRDLEDSHEKSRDLDDKLRKMELTDEEKEEDRKKEQKTLNEERMKLMEQKEEAMLVATKHATTIDQQTRRISVLEGDVEKLTAGIAERESSINALESNTMELISKLETTEAELEKLKDELAVMLKQNSELKNGKEGLSEKWNEERKKIQDLADQLREANKVVHNMRMKNVNLEEKKNELDQNVTDLTNKVRQLEIQLMDKAAKNEVSGDLLRKMEHDAQSMLKQAQNEQFRLTDLEKVRKALQDENQRLVNDLATVKAAFEVKRETSKSAISDILDKYRSAEEKANKGELDNQRLRSDLATVTLKLERQELKAKDSDNRLRDSQKRFEEVQSKLANLQKSAVESLQNPMSSNSRQNRSIYVDIPRAASSIGLNENSDEVPLRSSPSVRFADSSQNMQRAVDSMDVSSSVGVTLRFLKERIEQLEADNADLSDALEKAKDELRQRNEKLADRQMVIERVERQLVHITEERNTIENRMTSQRQMYLTNEESSRSREHEIRSMKARISTLELHLREKESKLAHLRKEIEVLHGQLHDALESKEKATGLVGVQDSKHRDLEEQLDRANRERELAIGKQRRTLAENENLFRKLEQLEKEREQLMREITDERRLNERNRTSLEELRVSERTWKSAMTTAKKPAEEQERAVQEQRRWEESNHEMTNRNTALTKECDRLRVEMRDQLNRMNGINLRSVDFERKNEELSSKLIVMQNTVTAMKKFEEEWKRLEAEMRAELKILRKEKLMQTAEIEDLKRKSFRSDTEKKEIEGIRVRLEREISALKRHVDALEEEKGKTEKAVRETMNERRAIDKSLASMERENQQLYRNCAQLQAQIQNLERDAGNRSVTKLAKEHSLLEARIAALIEEKRQLQSMLDQKDANYSHKRKLLESQIQLLREQLEAERRKRTKGVVATGPTVSRRGVQHTSAFRHTIERHRSLSQSSERTILQERYLEYVYTGDRTPAIQMINTPPISPLSHSGSFNSDGREARIRRESDNGHLQH

Target ORF information:

RefSeq Version	NM_182228.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Rootletin (che-10), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182228.4

ORF Insert Sequence:

ATGGATGTGG CACGTAAAAT GCGGAAGCTG CTGACCACCA AGAACGGGGA AATCGATGAA 
TCAAGGGAAG CAGCGAAGCA GGCAGAAAAG GAAAGAGATC GTGCAAAGAA GGATTTGGAA 
AAAGAAGAGA AACGAAGAAA AGATGACAGA GAAGCAGAGA GAAAACGATC AAGTGTCTAT 
TCACAGAGAG AACATGACCT GAAAAAGTTG GATGATGAGT TGAGGAAGGC CAGTGAAAAG 
ATCCGGAATC TCGAGGAGCA ACGGGAGAGC CAGGAAAAAC TCACCATATC AGTTCAGAAC 
TCATTGAATG AAGCACACAG GCAACACAAA CAGTTTATTG AGGAATTGAT GATTCGGCAT 
CGAGAAGAAC TGAAAGAGCG GGAGGATTCT CACGAGGAAG CGTTACGTTC CAAAGATACC 
GAGGAAAGAT CTCGATTTGA AAAAGAACGT TCTGAACGGG AAAAGATTAG ACGAGAAAGT 
GATGAGCTCA GAGAGACGCA GAGAAGTTTA AAGGGTGACG TGGCGGCGAT GAAGACAGAT 
TTGGATGATA AGACTTTAAG GCTTGACATG CTCGAAACAG AACGGGATGA GCTCAAAAAG 
AAACTGGAAA CCGAAAGAGA GCAGGCTGAT CAGAGAGACT TGGAGATAGC AGAGTGTCGA 
GCAAAGCTTG ATGAGATGGC TGAGAAGGAA GCGGAGCTCC GGAAAGAGCT CGCAGAGTTC 
CAGGCAATTA TCACTGCGAT GGAAGGAGAA GGAAAGCTGA ATCAAGAACA GTTTTTGGAG 
AGTAAGAATG AGTTGAACAC GCTGACAGAT CAAATTGAAT CTTTGAATAG TGAGGTGGAG 
AATAAGAATG AGGAAATTCG AAATTTGATG GCTACACTTC AAGAAAAAGA AGTTCACATT 
CAAAACGTCC GAACATCAAG TCATCAGTTG ACTGCCACAT ACGAAGAGGC TAACGGGGAA 
ATCGATATTT TGAAGGCTGA ACTTACCCGG CTCCACGAGC AAGTCAATGA GAGGACTCGA 
CAGATCTCCG AAGCTAATGA GAAGTATGAT GATGCAGCTC GGAAGAATGA CGCATTGCTC 
GAAGATGTCG CCACGTGGCA AGAGAAGTAT GAGCAGTTGA AGATGGAGCT GGAAGAAATG 
AACAGAAGAG GACAGGAGAA GGAACGAGAA GAAGCAGATC TTCGAGCATT ACTTGATGAT 
TTACGTGGAA ACTTTGATAA GTTGACTAAT GAGCTGAAGC AGAAGGGTGT TACTGTTGAT 
AGCTTGAATG AAGAAATTTC ATCGTTGAAA GAACAATTAA ACAAGTCAGA AAAGGAGAGA 
AAAGAAGAAC TGTTAAGGAT GGAAGAGCTT GAACAGAAAA ATGAAGCGGA AATGAAAGAA 
GAGTATGAAG TAAAGTTACA ATTGGCTGAG AAGGATCGTC AAGGAGTTGA GAATTTTGGA 
AAAGAATGTG AAGCGAGAAT GAATGAGCTC ACGAAGATCC ACGAGATGCT AATGGAAGAG 
CACGATCAGT TGAAAGTGGA TCATCTGCAC ACGGAAGAAG AAGTGGAGAG GTTGAAAGAG 
AAAATGAGAA AGGAATTGGA GAAGTTGAAT GAGCAGAATG ATGGTGATCG GGCGGAATGG 
AGTAATGAAA GGAATCGGCT GGAATCATCC AAAAACGAAG CAGTTACGGA ACTTCAAGAA 
CGAGTGCAGA AGCTTGAGGA TGTGGTGAAG GAGAAAGAAG ATAAGGAAAT TGCTCTTCGT 
AGGGATTTAG AAGACAGCCA TGAGAAATCA AGAGATCTTG ATGACAAGCT TCGAAAAATG 
GAGTTGACAG ATGAGGAGAA AGAGGAAGAT CGGAAGAAGG AACAGAAGAC ATTGAATGAA 
GAGAGGATGA AACTCATGGA ACAGAAAGAA GAAGCAATGC TTGTTGCTAC AAAACATGCA 
ACAACGATTG ATCAACAGAC TCGAAGAATT TCAGTGTTGG AGGGTGACGT GGAAAAGCTG 
ACGGCTGGAA TTGCGGAGAG AGAAAGTTCG ATTAATGCTT TGGAATCGAA TACAATGGAG 
CTGATATCCA AGCTGGAAAC TACTGAAGCA GAACTTGAAA AGCTGAAAGA CGAGCTTGCA 
GTCATGCTGA AACAAAACTC GGAACTCAAA AATGGAAAAG AGGGGTTGAG TGAGAAGTGG 
AATGAGGAGA GGAAGAAGAT CCAAGACTTG GCAGATCAAT TGAGAGAGGC TAACAAAGTT 
GTTCATAATA TGAGAATGAA GAATGTTAAT CTAGAAGAAA AGAAGAATGA ATTGGATCAG 
AATGTGACTG ATTTGACCAA TAAAGTTCGA CAATTAGAGA TTCAGTTGAT GGATAAAGCT 
GCGAAGAACG AGGTTTCAGG AGATTTGCTC AGAAAGATGG AGCATGATGC TCAGAGTATG 
CTGAAGCAAG CACAGAATGA GCAGTTTAGA CTAACTGATC TTGAAAAAGT TCGAAAAGCT 
CTTCAAGATG AGAATCAACG GCTTGTAAAT GATCTGGCAA CAGTGAAAGC TGCATTTGAA 
GTCAAGAGAG AAACGAGCAA ATCTGCAATC AGTGACATTT TGGATAAGTA CCGGAGTGCT 
GAGGAGAAGG CCAATAAGGG TGAATTGGAT AATCAGAGGC TTAGAAGTGA TCTGGCTACT 
GTGACATTAA AACTGGAACG TCAAGAACTG AAAGCAAAAG ATTCTGATAA TCGACTGAGA 
GATAGCCAAA AACGATTCGA AGAAGTTCAA TCAAAACTTG CAAATCTTCA GAAATCAGCA 
GTAGAATCCT TACAAAATCC TATGTCTTCG AATTCCCGCC AAAATCGGTC AATCTATGTT 
GACATTCCAC GTGCAGCTTC TTCGATCGGC CTCAATGAGA ATTCTGATGA AGTCCCGCTT 
CGCAGTTCCC CCTCAGTCCG TTTTGCAGAC AGCTCACAGA ATATGCAAAG AGCAGTTGAC 
TCTATGGATG TGAGTTCTTC AGTTGGAGTT ACACTTCGGT TTTTGAAAGA AAGAATCGAA 
CAGTTGGAAG CAGACAATGC AGATTTATCA GATGCTCTGG AAAAGGCAAA AGATGAGCTG 
AGGCAGAGAA ATGAGAAGCT GGCGGATAGG CAAATGGTTA TTGAGAGAGT TGAGAGACAA 
TTGGTTCATA TTACTGAAGA ACGCAATACA ATTGAAAATC GAATGACAAG TCAGCGTCAA 
ATGTATCTCA CAAATGAGGA ATCATCACGA TCAAGAGAAC ATGAAATCAG AAGTATGAAA 
GCCAGAATTT CAACTTTAGA GCTTCATCTT CGAGAAAAAG AATCTAAACT TGCACATCTA 
CGAAAAGAAA TTGAAGTACT TCATGGTCAG CTTCATGATG CTCTCGAATC AAAGGAAAAG 
GCTACTGGAT TGGTTGGAGT TCAAGATTCA AAGCATCGAG ACTTAGAAGA ACAACTCGAT 
CGAGCAAATC GTGAACGAGA ATTGGCAATT GGGAAGCAGA GAAGAACTCT TGCAGAGAAC 
GAGAATCTGT TCCGGAAGCT TGAACAATTG GAAAAGGAAA GAGAGCAGCT TATGCGAGAG 
ATCACAGATG AACGGAGGCT CAACGAGCGG AATCGCACAA GTCTTGAAGA ACTTCGAGTC 
TCTGAAAGAA CATGGAAATC AGCCATGACA ACTGCCAAAA AACCAGCTGA GGAGCAGGAA 
CGGGCGGTGC AGGAGCAACG GAGATGGGAA GAGTCAAATC ATGAAATGAC GAATAGAAAT 
ACTGCATTGA CAAAAGAATG TGATAGATTA CGAGTTGAAA TGAGAGATCA ACTCAACAGA 
ATGAACGGGA TCAATTTGAG ATCAGTAGAT TTCGAGAGAA AGAATGAAGA GCTTTCAAGT 
AAACTGATTG TTATGCAAAA TACAGTAACT GCGATGAAAA AGTTCGAGGA AGAATGGAAG 
AGGTTGGAGG CGGAGATGAG AGCAGAACTT AAGATTTTGA GAAAAGAAAA GCTCATGCAA 
ACTGCTGAAA TTGAGGATTT AAAGAGGAAA AGCTTCAGAA GTGACACTGA AAAGAAGGAG 
ATCGAGGGTA TTCGAGTAAG GTTGGAAAGG GAAATCAGTG CACTTAAGAG ACATGTTGAT 
GCGCTAGAAG AAGAGAAGGG AAAAACTGAA AAGGCCGTTC GAGAAACCAT GAACGAACGT 
CGTGCCATCG ACAAATCTCT CGCGTCAATG GAACGAGAAA ATCAACAGTT GTATAGGAAT 
TGTGCTCAAC TTCAAGCTCA AATTCAGAAT CTGGAGCGCG ACGCCGGAAA TCGGTCGGTA 
ACCAAGTTGG CTAAGGAGCA TTCATTGCTC GAAGCTCGAA TTGCAGCTTT AATTGAAGAA 
AAACGTCAGC TCCAATCAAT GCTTGACCAA AAAGACGCCA ACTACTCTCA CAAGCGAAAA 
CTCTTAGAAT CCCAAATCCA GTTGCTCCGC GAGCAACTCG AAGCCGAACG CCGAAAGCGT 
ACAAAGGGAG TTGTGGCGAC TGGTCCCACC GTTAGCCGAC GGGGCGTTCA GCACACATCT 
GCCTTTAGGC ACACCATCGA GCGTCATCGG TCTCTGTCCC AGTCTAGTGA ACGGACAATT 
CTTCAGGAAC GATACCTTGA ATATGTATAT ACAGGAGATC GTACTCCAGC AATACAAATG 
ATCAACACAC CGCCAATCTC TCCATTATCA CATTCTGGCT CTTTCAATTC GGACGGAAGG 
GAAGCCAGAA TCCGGAGAGA GTCTGACAAC GGCCATCTTC AGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

che-10 ( NM_062420.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_494821.3 Caenorhabditis elegans Rootletin (che-10), mRNA.

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CloneID	OCa339202
Clone ID Related Accession (Same CDS sequence)	NM_062420.4 , NM_062420.3
Accession Version	NM_062420.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Rootletin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_062420.3.

ATGAAATCGG CGGCAAGTTC GGAGGATGTC GTTCGTGTCA CTGAAGATCT ATCCGAATGC 
CGAAATCGGT TGGATGCTGG GATTGAGGAG AATCGGAGGA ATCGGCAGGT TATCCAGGAT 
ATTAATGATC AGCTCCAACG CTTCCGCCAA AGAGCAAACG CAGAATCAAT CGAGTCTTTC 
AATCTGACCC CCAGCCCCGA TGTGACATTA TCATCACTTG CTCATCCAGG ACTCACACAT 
CTTCACAATC AGACAAACAT TTCCATGCCT TCTCTAACCA TTGACATTCC GTTGAACTCC 
AATGCCATGA TTAGTTCATC TAGAACCCCA AACTATGCGA TTAATGGGTT AAGAAATCGA 
CACAAAAGTC TAGTAGGGCA TAGGTATCGG TCGACGAGCC CGATCGGAGA TTATGGAAGA 
CATCGAAGCA GTCCAAGAGT TTTGGCTCAT TATAATTTGG ATGGTGCAGA TATTGGAGTT 
GGAGAGGAGA ATTTGGACGA ACTTTTTGCA AAGTTGAAAG AGGAATTGTT CAAAAACAAC 
ACACTGGAAG AGGTGAATGA GATGCTCCGA GAAGAAAATG ATGCAGCACT AGCTGCAAAT 
GAGCATTTAA GAGTTGATGC TACTAATTTG AGCAGACAAC TACAGCAATT ACAACAGCAG 
CAGCATACAG AGTCGATGAG ATTTCGGTCG GAGAATACGC GCTATCGAAA TCAAACGGAG 
ACCCAACACA GAAAGCTGAT CAGTCTTTGG AAGGAGTTCA CCGCTGTCAA GCGTCAGCTC 
CACGAGTTGC GCACAACCAC CGCCAACGAT TTGGACAGAC AATTGACCGA ATTCACGCGG 
TGTGCAACAC TTATGCGAAA AGCCATTCGC CACGCCGAGC AAAAGAATCT CGACCAGAAG 
GAACAAATGA AACGAGAAAA GGACGATGTA CTCGATGAGA CACTTCGTCA GCTCAACTCT 
GTCACTGAGA ATTATATGAA ATCAGAAGAA AAAGCAAACG AACGGCAGCG AGATTTGAAG 
AGAAAAGAGG ACGAATGCAG GAAGCTCCGC GAGCAAAACG ACGAGCTCTC GGATATTCTC 
GAACAACTTT CCAAAATGGC TCACGAAATG GCTGGAGGCA GAGGAAGAAA CGAAACACCA 
ATGGATGTGG CACGTAAAAT GCGGAAGCTG CTGACCACCA AGAACGGGGA AATCGATGAA 
TCAAGGGAAG CAGCGAAGCA GGCAGAAAAG GAAAGAGATC GTGCAAAGAA GGATTTGGAA 
AAAGAAGAGA AACGAAGAAA AGATGACAGA GAAGCAGAGA GAAAACGATC AAGTGTCTAT 
TCACAGAGAG AACATGACCT GAAAAAGTTG GATGATGAGT TGAGGAAGGC CAGTGAAAAG 
ATCCGGAATC TCGAGGAGCA ACGGGAGAGC CAGGAAAAAC TCACCATATC AGTTCAGAAC 
TCATTGAATG AAGCACACAG GCAACACAAA CAGTTTATTG AGGAATTGAT GATTCGGCAT 
CGAGAAGAAC TGAAAGAGCG GGAGGATTCT CACGAGGAAG CGTTACGTTC CAAAGATACC 
GAGGAAAGAT CTCGATTTGA AAAAGAACGT TCTGAACGGG AAAAGATTAG ACGAGAAAGT 
GATGAGCTCA GAGAGACGCA GAGAAGTTTA AAGGGTGACG TGGCGGCGAT GAAGACAGAT 
TTGGATGATA AGACTTTAAG GCTTGACATG CTCGAAACAG AACGGGATGA GCTCAAAAAG 
AAACTGGAAA CCGAAAGAGA GCAGGCTGAT CAGAGAGACT TGGAGATAGC AGAGTGTCGA 
GCAAAGCTTG ATGAGATGGC TGAGAAGGAA GCGGAGCTCC GGAAAGAGCT CGCAGAGTTC 
CAGGCAATTA TCACTGCGAT GGAAGGAGAA GGAAAGCTGA ATCAAGAACA GTTTTTGGAG 
AGTAAGAATG AGTTGAACAC GCTGACAGAT CAAATTGAAT CTTTGAATAG TGAGGTGGAG 
AATAAGAATG AGGAAATTCG AAATTTGATG GCTACACTTC AAGAAAAAGA AGTTCACATT 
CAAAACGTCC GAACATCAAG TCATCAGTTG ACTGCCACAT ACGAAGAGGC TAACGGGGAA 
ATCGATATTT TGAAGGCTGA ACTTACCCGG CTCCACGAGC AAGTCAATGA GAGGACTCGA 
CAGATCTCCG AAGCTAATGA GAAGTATGAT GATGCAGCTC GGAAGAATGA CGCATTGCTC 
GAAGATGTCG CCACGTGGCA AGAGAAGTAT GAGCAGTTGA AGATGGAGCT GGAAGAAATG 
AACAGAAGAG GACAGGAGAA GGAACGAGAA GAAGCAGATC TTCGAGCATT ACTTGATGAT 
TTACGTGGAA ACTTTGATAA GTTGACTAAT GAGCTGAAGC AGAAGGGTGT TACTGTTGAT 
AGCTTGAATG AAGAAATTTC ATCGTTGAAA GAACAATTAA ACAAGTCAGA AAAGGAGAGA 
AAAGAAGAAC TGTTAAGGAT GGAAGAGCTT GAACAGAAAA ATGAAGCGGA AATGAAAGAA 
GAGTATGAAG TAAAGTTACA ATTGGCTGAG AAGGATCGTC AAGGAGTTGA GAATTTTGGA 
AAAGAATGTG AAGCGAGAAT GAATGAGCTC ACGAAGATCC ACGAGATGCT AATGGAAGAG 
CACGATCAGT TGAAAGTGGA TCATCTGCAC ACGGAAGAAG AAGTGGAGAG GTTGAAAGAG 
AAAATGAGAA AGGAATTGGA GAAGTTGAAT GAGCAGAATG ATGGTGATCG GGCGGAATGG 
AGTAATGAAA GGAATCGGCT GGAATCATCC AAAAACGAAG CAGTTACGGA ACTTCAAGAA 
CGAGTGCAGA AGCTTGAGGA TGTGGTGAAG GAGAAAGAAG ATAAGGAAAT TGCTCTTCGT 
AGGGATTTAG AAGACAGCCA TGAGAAATCA AGAGATCTTG ATGACAAGCT TCGAAAAATG 
GAGTTGACAG ATGAGGAGAA AGAGGAAGAT CGGAAGAAGG AACAGAAGAC ATTGAATGAA 
GAGAGGATGA AACTCATGGA ACAGAAAGAA GAAGCAATGC TTGTTGCTAC AAAACATGCA 
ACAACGATTG ATCAACAGAC TCGAAGAATT TCAGTGTTGG AGGGTGACGT GGAAAAGCTG 
ACGGCTGGAA TTGCGGAGAG AGAAAGTTCG ATTAATGCTT TGGAATCGAA TACAATGGAG 
CTGATATCCA AGCTGGAAAC TACTGAAGCA GAACTTGAAA AGCTGAAAGA CGAGCTTGCA 
GTCATGCTGA AACAAAACTC GGAACTCAAA AATGGAAAAG AGGGGTTGAG TGAGAAGTGG 
AATGAGGAGA GGAAGAAGAT CCAAGACTTG GCAGATCAAT TGAGAGAGGC TAACAAAGTT 
GTTCATAATA TGAGAATGAA GAATGTTAAT CTAGAAGAAA AGAAGAATGA ATTGGATCAG 
AATGTGACTG ATTTGACCAA TAAAGTTCGA CAATTAGAGA TTCAGTTGAT GGATAAAGCT 
GCGAAGAACG AGGTTTCAGG AGATTTGCTC AGAAAGATGG AGCATGATGC TCAGAGTATG 
CTGAAGCAAG CACAGAATGA GCAGTTTAGA CTAACTGATC TTGAAAAAGT TCGAAAAGCT 
CTTCAAGATG AGAATCAACG GCTTGTAAAT GATCTGGCAA CAGTGAAAGC TGCATTTGAA 
GTCAAGAGAG AAACGAGCAA ATCTGCAATC AGTGACATTT TGGATAAGTA CCGGAGTGCT 
GAGGAGAAGG CCAATAAGGG TGAATTGGAT AATCAGAGGC TTAGAAGTGA TCTGGCTACT 
GTGACATTAA AACTGGAACG TCAAGAACTG AAAGCAAAAG ATTCTGATAA TCGACTGAGA 
GATAGCCAAA AACGATTCGA AGAAGTTCAA TCAAAACTTG CAAATCTTCA GAAATCAGCA 
GTAGAATCCT TACAAAATCC TATGTCTTCG AATTCCCGCC AAAATCGGTC AATCTATGTT 
GACATTCCAC GTGCAGCTTC TTCGATCGGC CTCAATGAGA ATTCTGATGA AGTCCCGCTT 
CGCAGTTCCC CCTCAGTCCG TTTTGCAGAC AGCTCACAGA ATATGCAAAG AGCAGTTGAC 
TCTATGGATG TGAGTTCTTC AGTTGGAGTT ACACTTCGGT TTTTGAAAGA AAGAATCGAA 
CAGTTGGAAG CAGACAATGC AGATTTATCA GATGCTCTGG AAAAGGCAAA AGATGAGCTG 
AGGCAGAGAA ATGAGAAGCT GGCGGATAGG CAAATGGTTA TTGAGAGAGT TGAGAGACAA 
TTGGTTCATA TTACTGAAGA ACGCAATACA ATTGAAAATC GAATGACAAG TCAGCGTCAA 
ATGTATCTCA CAAATGAGGA ATCATCACGA TCAAGAGAAC ATGAAATCAG AAGTATGAAA 
GCCAGAATTT CAACTTTAGA GCTTCATCTT CGAGAAAAAG AATCTAAACT TGCACATCTA 
CGAAAAGAAA TTGAAGTACT TCATGGTCAG CTTCATGATG CTCTCGAATC AAAGGAAAAG 
GCTACTGGAT TGGTTGGAGT TCAAGATTCA AAGCATCGAG ACTTAGAAGA ACAACTCGAT 
CGAGCAAATC GTGAACGAGA ATTGGCAATT GGGAAGCAGA GAAGAACTCT TGCAGAGAAC 
GAGAATCTGT TCCGGAAGCT TGAACAATTG GAAAAGGAAA GAGAGCAGCT TATGCGAGAG 
ATCACAGATG AACGGAGGCT CAACGAGCGG AATCGCACAA GTCTTGAAGA ACTTCGAGTC 
TCTGAAAGAA CATGGAAATC AGCCATGACA ACTGCCAAAA AACCAGCTGA GGAGCAGGAA 
CGGGCGGTGC AGGAGCAACG GAGATGGGAA GAGTCAAATC ATGAAATGAC GAATAGAAAT 
ACTGCATTGA CAAAAGAATG TGATAGATTA CGAGTTGAAA TGAGAGATCA ACTCAACAGA 
ATGAACGGGA TCAATTTGAG ATCAGTAGAT TTCGAGAGAA AGAATGAAGA GCTTTCAAGT 
AAACTGATTG TTATGCAAAA TACAGTAACT GCGATGAAAA AGTTCGAGGA AGAATGGAAG 
AGGTTGGAGG CGGAGATGAG AGCAGAACTT AAGATTTTGA GAAAAGAAAA GCTCATGCAA 
ACTGCTGAAA TTGAGGATTT AAAGAGGAAA AGCTTCAGAA GTGACACTGA AAAGAAGGAG 
ATCGAGGGTA TTCGAGTAAG GTTGGAAAGG GAAATCAGTG CACTTAAGAG ACATGTTGAT 
GCGCTAGAAG AAGAGAAGGG AAAAACTGAA AAGGCCGTTC GAGAAACCAT GAACGAACGT 
CGTGCCATCG ACAAATCTCT CGCGTCAATG GAACGAGAAA ATCAACAGTT GTATAGGAAT 
TGTGCTCAAC TTCAAGCTCA AATTCAGAAT CTGGAGCGCG ACGCCGGAAA TCGGTCGGTA 
ACCAAGTTGG CTAAGGAGCA TTCATTGCTC GAAGCTCGAA TTGCAGCTTT AATTGAAGAA 
AAACGTCAGC TCCAATCAAT GCTTGACCAA AAAGACGCCA ACTACTCTCA CAAGCGAAAA 
CTCTTAGAAT CCCAAATCCA GTTGCTCCGC GAGCAACTCG AAGCCGAACG CCGAAAGCGT 
ACAAAGGGAG TTGTGGCGAC TGGTCCCACC GTTAGCCGAC GGGGCGTTCA GCACACATCT 
GCCTTTAGGG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_494821.3
CDS	44..5698
Translation	MKSAASSEDVVRVTEDLSECRNRLDAGIEENRRNRQVIQDINDQLQRFRQRANAESIESFNLTPSPDVTLSSLAHPGLTHLHNQTNISMPSLTIDIPLNSNAMISSSRTPNYAINGLRNRHKSLVGHRYRSTSPIGDYGRHRSSPRVLAHYNLDGADIGVGEENLDELFAKLKEELFKNNTLEEVNEMLREENDAALAANEHLRVDATNLSRQLQQLQQQQHTESMRFRSENTRYRNQTETQHRKLISLWKEFTAVKRQLHELRTTTANDLDRQLTEFTRCATLMRKAIRHAEQKNLDQKEQMKREKDDVLDETLRQLNSVTENYMKSEEKANERQRDLKRKEDECRKLREQNDELSDILEQLSKMAHEMAGGRGRNETPMDVARKMRKLLTTKNGEIDESREAAKQAEKERDRAKKDLEKEEKRRKDDREAERKRSSVYSQREHDLKKLDDELRKASEKIRNLEEQRESQEKLTISVQNSLNEAHRQHKQFIEELMIRHREELKEREDSHEEALRSKDTEERSRFEKERSEREKIRRESDELRETQRSLKGDVAAMKTDLDDKTLRLDMLETERDELKKKLETEREQADQRDLEIAECRAKLDEMAEKEAELRKELAEFQAIITAMEGEGKLNQEQFLESKNELNTLTDQIESLNSEVENKNEEIRNLMATLQEKEVHIQNVRTSSHQLTATYEEANGEIDILKAELTRLHEQVNERTRQISEANEKYDDAARKNDALLEDVATWQEKYEQLKMELEEMNRRGQEKEREEADLRALLDDLRGNFDKLTNELKQKGVTVDSLNEEISSLKEQLNKSEKERKEELLRMEELEQKNEAEMKEEYEVKLQLAEKDRQGVENFGKECEARMNELTKIHEMLMEEHDQLKVDHLHTEEEVERLKEKMRKELEKLNEQNDGDRAEWSNERNRLESSKNEAVTELQERVQKLEDVVKEKEDKEIALRRDLEDSHEKSRDLDDKLRKMELTDEEKEEDRKKEQKTLNEERMKLMEQKEEAMLVATKHATTIDQQTRRISVLEGDVEKLTAGIAERESSINALESNTMELISKLETTEAELEKLKDELAVMLKQNSELKNGKEGLSEKWNEERKKIQDLADQLREANKVVHNMRMKNVNLEEKKNELDQNVTDLTNKVRQLEIQLMDKAAKNEVSGDLLRKMEHDAQSMLKQAQNEQFRLTDLEKVRKALQDENQRLVNDLATVKAAFEVKRETSKSAISDILDKYRSAEEKANKGELDNQRLRSDLATVTLKLERQELKAKDSDNRLRDSQKRFEEVQSKLANLQKSAVESLQNPMSSNSRQNRSIYVDIPRAASSIGLNENSDEVPLRSSPSVRFADSSQNMQRAVDSMDVSSSVGVTLRFLKERIEQLEADNADLSDALEKAKDELRQRNEKLADRQMVIERVERQLVHITEERNTIENRMTSQRQMYLTNEESSRSREHEIRSMKARISTLELHLREKESKLAHLRKEIEVLHGQLHDALESKEKATGLVGVQDSKHRDLEEQLDRANRERELAIGKQRRTLAENENLFRKLEQLEKEREQLMREITDERRLNERNRTSLEELRVSERTWKSAMTTAKKPAEEQERAVQEQRRWEESNHEMTNRNTALTKECDRLRVEMRDQLNRMNGINLRSVDFERKNEELSSKLIVMQNTVTAMKKFEEEWKRLEAEMRAELKILRKEKLMQTAEIEDLKRKSFRSDTEKKEIEGIRVRLEREISALKRHVDALEEEKGKTEKAVRETMNERRAIDKSLASMERENQQLYRNCAQLQAQIQNLERDAGNRSVTKLAKEHSLLEARIAALIEEKRQLQSMLDQKDANYSHKRKLLESQIQLLREQLEAERRKRTKGVVATGPTVSRRGVQHTSAFRV

Target ORF information:

RefSeq Version	NM_062420.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Rootletin (che-10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_062420.4

ORF Insert Sequence:

ATGAAATCGG CGGCAAGTTC GGAGGATGTC GTTCGTGTCA CTGAAGATCT ATCCGAATGC 
CGAAATCGGT TGGATGCTGG GATTGAGGAG AATCGGAGGA ATCGGCAGGT TATCCAGGAT 
ATTAATGATC AGCTCCAACG CTTCCGCCAA AGAGCAAACG CAGAATCAAT CGAGTCTTTC 
AATCTGACCC CCAGCCCCGA TGTGACATTA TCATCACTTG CTCATCCAGG ACTCACACAT 
CTTCACAATC AGACAAACAT TTCCATGCCT TCTCTAACCA TTGACATTCC GTTGAACTCC 
AATGCCATGA TTAGTTCATC TAGAACCCCA AACTATGCGA TTAATGGGTT AAGAAATCGA 
CACAAAAGTC TAGTAGGGCA TAGGTATCGG TCGACGAGCC CGATCGGAGA TTATGGAAGA 
CATCGAAGCA GTCCAAGAGT TTTGGCTCAT TATAATTTGG ATGGTGCAGA TATTGGAGTT 
GGAGAGGAGA ATTTGGACGA ACTTTTTGCA AAGTTGAAAG AGGAATTGTT CAAAAACAAC 
ACACTGGAAG AGGTGAATGA GATGCTCCGA GAAGAAAATG ATGCAGCACT AGCTGCAAAT 
GAGCATTTAA GAGTTGATGC TACTAATTTG AGCAGACAAC TACAGCAATT ACAACAGCAG 
CAGCATACAG AGTCGATGAG ATTTCGGTCG GAGAATACGC GCTATCGAAA TCAAACGGAG 
ACCCAACACA GAAAGCTGAT CAGTCTTTGG AAGGAGTTCA CCGCTGTCAA GCGTCAGCTC 
CACGAGTTGC GCACAACCAC CGCCAACGAT TTGGACAGAC AATTGACCGA ATTCACGCGG 
TGTGCAACAC TTATGCGAAA AGCCATTCGC CACGCCGAGC AAAAGAATCT CGACCAGAAG 
GAACAAATGA AACGAGAAAA GGACGATGTA CTCGATGAGA CACTTCGTCA GCTCAACTCT 
GTCACTGAGA ATTATATGAA ATCAGAAGAA AAAGCAAACG AACGGCAGCG AGATTTGAAG 
AGAAAAGAGG ACGAATGCAG GAAGCTCCGC GAGCAAAACG ACGAGCTCTC GGATATTCTC 
GAACAACTTT CCAAAATGGC TCACGAAATG GCTGGAGGCA GAGGAAGAAA CGAAACACCA 
ATGGATGTGG CACGTAAAAT GCGGAAGCTG CTGACCACCA AGAACGGGGA AATCGATGAA 
TCAAGGGAAG CAGCGAAGCA GGCAGAAAAG GAAAGAGATC GTGCAAAGAA GGATTTGGAA 
AAAGAAGAGA AACGAAGAAA AGATGACAGA GAAGCAGAGA GAAAACGATC AAGTGTCTAT 
TCACAGAGAG AACATGACCT GAAAAAGTTG GATGATGAGT TGAGGAAGGC CAGTGAAAAG 
ATCCGGAATC TCGAGGAGCA ACGGGAGAGC CAGGAAAAAC TCACCATATC AGTTCAGAAC 
TCATTGAATG AAGCACACAG GCAACACAAA CAGTTTATTG AGGAATTGAT GATTCGGCAT 
CGAGAAGAAC TGAAAGAGCG GGAGGATTCT CACGAGGAAG CGTTACGTTC CAAAGATACC 
GAGGAAAGAT CTCGATTTGA AAAAGAACGT TCTGAACGGG AAAAGATTAG ACGAGAAAGT 
GATGAGCTCA GAGAGACGCA GAGAAGTTTA AAGGGTGACG TGGCGGCGAT GAAGACAGAT 
TTGGATGATA AGACTTTAAG GCTTGACATG CTCGAAACAG AACGGGATGA GCTCAAAAAG 
AAACTGGAAA CCGAAAGAGA GCAGGCTGAT CAGAGAGACT TGGAGATAGC AGAGTGTCGA 
GCAAAGCTTG ATGAGATGGC TGAGAAGGAA GCGGAGCTCC GGAAAGAGCT CGCAGAGTTC 
CAGGCAATTA TCACTGCGAT GGAAGGAGAA GGAAAGCTGA ATCAAGAACA GTTTTTGGAG 
AGTAAGAATG AGTTGAACAC GCTGACAGAT CAAATTGAAT CTTTGAATAG TGAGGTGGAG 
AATAAGAATG AGGAAATTCG AAATTTGATG GCTACACTTC AAGAAAAAGA AGTTCACATT 
CAAAACGTCC GAACATCAAG TCATCAGTTG ACTGCCACAT ACGAAGAGGC TAACGGGGAA 
ATCGATATTT TGAAGGCTGA ACTTACCCGG CTCCACGAGC AAGTCAATGA GAGGACTCGA 
CAGATCTCCG AAGCTAATGA GAAGTATGAT GATGCAGCTC GGAAGAATGA CGCATTGCTC 
GAAGATGTCG CCACGTGGCA AGAGAAGTAT GAGCAGTTGA AGATGGAGCT GGAAGAAATG 
AACAGAAGAG GACAGGAGAA GGAACGAGAA GAAGCAGATC TTCGAGCATT ACTTGATGAT 
TTACGTGGAA ACTTTGATAA GTTGACTAAT GAGCTGAAGC AGAAGGGTGT TACTGTTGAT 
AGCTTGAATG AAGAAATTTC ATCGTTGAAA GAACAATTAA ACAAGTCAGA AAAGGAGAGA 
AAAGAAGAAC TGTTAAGGAT GGAAGAGCTT GAACAGAAAA ATGAAGCGGA AATGAAAGAA 
GAGTATGAAG TAAAGTTACA ATTGGCTGAG AAGGATCGTC AAGGAGTTGA GAATTTTGGA 
AAAGAATGTG AAGCGAGAAT GAATGAGCTC ACGAAGATCC ACGAGATGCT AATGGAAGAG 
CACGATCAGT TGAAAGTGGA TCATCTGCAC ACGGAAGAAG AAGTGGAGAG GTTGAAAGAG 
AAAATGAGAA AGGAATTGGA GAAGTTGAAT GAGCAGAATG ATGGTGATCG GGCGGAATGG 
AGTAATGAAA GGAATCGGCT GGAATCATCC AAAAACGAAG CAGTTACGGA ACTTCAAGAA 
CGAGTGCAGA AGCTTGAGGA TGTGGTGAAG GAGAAAGAAG ATAAGGAAAT TGCTCTTCGT 
AGGGATTTAG AAGACAGCCA TGAGAAATCA AGAGATCTTG ATGACAAGCT TCGAAAAATG 
GAGTTGACAG ATGAGGAGAA AGAGGAAGAT CGGAAGAAGG AACAGAAGAC ATTGAATGAA 
GAGAGGATGA AACTCATGGA ACAGAAAGAA GAAGCAATGC TTGTTGCTAC AAAACATGCA 
ACAACGATTG ATCAACAGAC TCGAAGAATT TCAGTGTTGG AGGGTGACGT GGAAAAGCTG 
ACGGCTGGAA TTGCGGAGAG AGAAAGTTCG ATTAATGCTT TGGAATCGAA TACAATGGAG 
CTGATATCCA AGCTGGAAAC TACTGAAGCA GAACTTGAAA AGCTGAAAGA CGAGCTTGCA 
GTCATGCTGA AACAAAACTC GGAACTCAAA AATGGAAAAG AGGGGTTGAG TGAGAAGTGG 
AATGAGGAGA GGAAGAAGAT CCAAGACTTG GCAGATCAAT TGAGAGAGGC TAACAAAGTT 
GTTCATAATA TGAGAATGAA GAATGTTAAT CTAGAAGAAA AGAAGAATGA ATTGGATCAG 
AATGTGACTG ATTTGACCAA TAAAGTTCGA CAATTAGAGA TTCAGTTGAT GGATAAAGCT 
GCGAAGAACG AGGTTTCAGG AGATTTGCTC AGAAAGATGG AGCATGATGC TCAGAGTATG 
CTGAAGCAAG CACAGAATGA GCAGTTTAGA CTAACTGATC TTGAAAAAGT TCGAAAAGCT 
CTTCAAGATG AGAATCAACG GCTTGTAAAT GATCTGGCAA CAGTGAAAGC TGCATTTGAA 
GTCAAGAGAG AAACGAGCAA ATCTGCAATC AGTGACATTT TGGATAAGTA CCGGAGTGCT 
GAGGAGAAGG CCAATAAGGG TGAATTGGAT AATCAGAGGC TTAGAAGTGA TCTGGCTACT 
GTGACATTAA AACTGGAACG TCAAGAACTG AAAGCAAAAG ATTCTGATAA TCGACTGAGA 
GATAGCCAAA AACGATTCGA AGAAGTTCAA TCAAAACTTG CAAATCTTCA GAAATCAGCA 
GTAGAATCCT TACAAAATCC TATGTCTTCG AATTCCCGCC AAAATCGGTC AATCTATGTT 
GACATTCCAC GTGCAGCTTC TTCGATCGGC CTCAATGAGA ATTCTGATGA AGTCCCGCTT 
CGCAGTTCCC CCTCAGTCCG TTTTGCAGAC AGCTCACAGA ATATGCAAAG AGCAGTTGAC 
TCTATGGATG TGAGTTCTTC AGTTGGAGTT ACACTTCGGT TTTTGAAAGA AAGAATCGAA 
CAGTTGGAAG CAGACAATGC AGATTTATCA GATGCTCTGG AAAAGGCAAA AGATGAGCTG 
AGGCAGAGAA ATGAGAAGCT GGCGGATAGG CAAATGGTTA TTGAGAGAGT TGAGAGACAA 
TTGGTTCATA TTACTGAAGA ACGCAATACA ATTGAAAATC GAATGACAAG TCAGCGTCAA 
ATGTATCTCA CAAATGAGGA ATCATCACGA TCAAGAGAAC ATGAAATCAG AAGTATGAAA 
GCCAGAATTT CAACTTTAGA GCTTCATCTT CGAGAAAAAG AATCTAAACT TGCACATCTA 
CGAAAAGAAA TTGAAGTACT TCATGGTCAG CTTCATGATG CTCTCGAATC AAAGGAAAAG 
GCTACTGGAT TGGTTGGAGT TCAAGATTCA AAGCATCGAG ACTTAGAAGA ACAACTCGAT 
CGAGCAAATC GTGAACGAGA ATTGGCAATT GGGAAGCAGA GAAGAACTCT TGCAGAGAAC 
GAGAATCTGT TCCGGAAGCT TGAACAATTG GAAAAGGAAA GAGAGCAGCT TATGCGAGAG 
ATCACAGATG AACGGAGGCT CAACGAGCGG AATCGCACAA GTCTTGAAGA ACTTCGAGTC 
TCTGAAAGAA CATGGAAATC AGCCATGACA ACTGCCAAAA AACCAGCTGA GGAGCAGGAA 
CGGGCGGTGC AGGAGCAACG GAGATGGGAA GAGTCAAATC ATGAAATGAC GAATAGAAAT 
ACTGCATTGA CAAAAGAATG TGATAGATTA CGAGTTGAAA TGAGAGATCA ACTCAACAGA 
ATGAACGGGA TCAATTTGAG ATCAGTAGAT TTCGAGAGAA AGAATGAAGA GCTTTCAAGT 
AAACTGATTG TTATGCAAAA TACAGTAACT GCGATGAAAA AGTTCGAGGA AGAATGGAAG 
AGGTTGGAGG CGGAGATGAG AGCAGAACTT AAGATTTTGA GAAAAGAAAA GCTCATGCAA 
ACTGCTGAAA TTGAGGATTT AAAGAGGAAA AGCTTCAGAA GTGACACTGA AAAGAAGGAG 
ATCGAGGGTA TTCGAGTAAG GTTGGAAAGG GAAATCAGTG CACTTAAGAG ACATGTTGAT 
GCGCTAGAAG AAGAGAAGGG AAAAACTGAA AAGGCCGTTC GAGAAACCAT GAACGAACGT 
CGTGCCATCG ACAAATCTCT CGCGTCAATG GAACGAGAAA ATCAACAGTT GTATAGGAAT 
TGTGCTCAAC TTCAAGCTCA AATTCAGAAT CTGGAGCGCG ACGCCGGAAA TCGGTCGGTA 
ACCAAGTTGG CTAAGGAGCA TTCATTGCTC GAAGCTCGAA TTGCAGCTTT AATTGAAGAA 
AAACGTCAGC TCCAATCAAT GCTTGACCAA AAAGACGCCA ACTACTCTCA CAAGCGAAAA 
CTCTTAGAAT CCCAAATCCA GTTGCTCCGC GAGCAACTCG AAGCCGAACG CCGAAAGCGT 
ACAAAGGGAG TTGTGGCGAC TGGTCCCACC GTTAGCCGAC GGGGCGTTCA GCACACATCT 
GCCTTTAGGG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

che-10 ( NM_062420.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_494821.3 Caenorhabditis elegans Rootletin (che-10), partial mRNA.

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CloneID	OCa339202
Clone ID Related Accession (Same CDS sequence)	NM_062420.4 , NM_062420.3
Accession Version	NM_062420.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Rootletin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Mar 21, 2007 this sequence version replaced NM_062420.2. COMPLETENESS: incomplete on both ends.

ATGAAATCGG CGGCAAGTTC GGAGGATGTC GTTCGTGTCA CTGAAGATCT ATCCGAATGC 
CGAAATCGGT TGGATGCTGG GATTGAGGAG AATCGGAGGA ATCGGCAGGT TATCCAGGAT 
ATTAATGATC AGCTCCAACG CTTCCGCCAA AGAGCAAACG CAGAATCAAT CGAGTCTTTC 
AATCTGACCC CCAGCCCCGA TGTGACATTA TCATCACTTG CTCATCCAGG ACTCACACAT 
CTTCACAATC AGACAAACAT TTCCATGCCT TCTCTAACCA TTGACATTCC GTTGAACTCC 
AATGCCATGA TTAGTTCATC TAGAACCCCA AACTATGCGA TTAATGGGTT AAGAAATCGA 
CACAAAAGTC TAGTAGGGCA TAGGTATCGG TCGACGAGCC CGATCGGAGA TTATGGAAGA 
CATCGAAGCA GTCCAAGAGT TTTGGCTCAT TATAATTTGG ATGGTGCAGA TATTGGAGTT 
GGAGAGGAGA ATTTGGACGA ACTTTTTGCA AAGTTGAAAG AGGAATTGTT CAAAAACAAC 
ACACTGGAAG AGGTGAATGA GATGCTCCGA GAAGAAAATG ATGCAGCACT AGCTGCAAAT 
GAGCATTTAA GAGTTGATGC TACTAATTTG AGCAGACAAC TACAGCAATT ACAACAGCAG 
CAGCATACAG AGTCGATGAG ATTTCGGTCG GAGAATACGC GCTATCGAAA TCAAACGGAG 
ACCCAACACA GAAAGCTGAT CAGTCTTTGG AAGGAGTTCA CCGCTGTCAA GCGTCAGCTC 
CACGAGTTGC GCACAACCAC CGCCAACGAT TTGGACAGAC AATTGACCGA ATTCACGCGG 
TGTGCAACAC TTATGCGAAA AGCCATTCGC CACGCCGAGC AAAAGAATCT CGACCAGAAG 
GAACAAATGA AACGAGAAAA GGACGATGTA CTCGATGAGA CACTTCGTCA GCTCAACTCT 
GTCACTGAGA ATTATATGAA ATCAGAAGAA AAAGCAAACG AACGGCAGCG AGATTTGAAG 
AGAAAAGAGG ACGAATGCAG GAAGCTCCGC GAGCAAAACG ACGAGCTCTC GGATATTCTC 
GAACAACTTT CCAAAATGGC TCACGAAATG GCTGGAGGCA GAGGAAGAAA CGAAACACCA 
ATGGATGTGG CACGTAAAAT GCGGAAGCTG CTGACCACCA AGAACGGGGA AATCGATGAA 
TCAAGGGAAG CAGCGAAGCA GGCAGAAAAG GAAAGAGATC GTGCAAAGAA GGATTTGGAA 
AAAGAAGAGA AACGAAGAAA AGATGACAGA GAAGCAGAGA GAAAACGATC AAGTGTCTAT 
TCACAGAGAG AACATGACCT GAAAAAGTTG GATGATGAGT TGAGGAAGGC CAGTGAAAAG 
ATCCGGAATC TCGAGGAGCA ACGGGAGAGC CAGGAAAAAC TCACCATATC AGTTCAGAAC 
TCATTGAATG AAGCACACAG GCAACACAAA CAGTTTATTG AGGAATTGAT GATTCGGCAT 
CGAGAAGAAC TGAAAGAGCG GGAGGATTCT CACGAGGAAG CGTTACGTTC CAAAGATACC 
GAGGAAAGAT CTCGATTTGA AAAAGAACGT TCTGAACGGG AAAAGATTAG ACGAGAAAGT 
GATGAGCTCA GAGAGACGCA GAGAAGTTTA AAGGGTGACG TGGCGGCGAT GAAGACAGAT 
TTGGATGATA AGACTTTAAG GCTTGACATG CTCGAAACAG AACGGGATGA GCTCAAAAAG 
AAACTGGAAA CCGAAAGAGA GCAGGCTGAT CAGAGAGACT TGGAGATAGC AGAGTGTCGA 
GCAAAGCTTG ATGAGATGGC TGAGAAGGAA GCGGAGCTCC GGAAAGAGCT CGCAGAGTTC 
CAGGCAATTA TCACTGCGAT GGAAGGAGAA GGAAAGCTGA ATCAAGAACA GTTTTTGGAG 
AGTAAGAATG AGTTGAACAC GCTGACAGAT CAAATTGAAT CTTTGAATAG TGAGGTGGAG 
AATAAGAATG AGGAAATTCG AAATTTGATG GCTACACTTC AAGAAAAAGA AGTTCACATT 
CAAAACGTCC GAACATCAAG TCATCAGTTG ACTGCCACAT ACGAAGAGGC TAACGGGGAA 
ATCGATATTT TGAAGGCTGA ACTTACCCGG CTCCACGAGC AAGTCAATGA GAGGACTCGA 
CAGATCTCCG AAGCTAATGA GAAGTATGAT GATGCAGCTC GGAAGAATGA CGCATTGCTC 
GAAGATGTCG CCACGTGGCA AGAGAAGTAT GAGCAGTTGA AGATGGAGCT GGAAGAAATG 
AACAGAAGAG GACAGGAGAA GGAACGAGAA GAAGCAGATC TTCGAGCATT ACTTGATGAT 
TTACGTGGAA ACTTTGATAA GTTGACTAAT GAGCTGAAGC AGAAGGGTGT TACTGTTGAT 
AGCTTGAATG AAGAAATTTC ATCGTTGAAA GAACAATTAA ACAAGTCAGA AAAGGAGAGA 
AAAGAAGAAC TGTTAAGGAT GGAAGAGCTT GAACAGAAAA ATGAAGCGGA AATGAAAGAA 
GAGTATGAAG TAAAGTTACA ATTGGCTGAG AAGGATCGTC AAGGAGTTGA GAATTTTGGA 
AAAGAATGTG AAGCGAGAAT GAATGAGCTC ACGAAGATCC ACGAGATGCT AATGGAAGAG 
CACGATCAGT TGAAAGTGGA TCATCTGCAC ACGGAAGAAG AAGTGGAGAG GTTGAAAGAG 
AAAATGAGAA AGGAATTGGA GAAGTTGAAT GAGCAGAATG ATGGTGATCG GGCGGAATGG 
AGTAATGAAA GGAATCGGCT GGAATCATCC AAAAACGAAG CAGTTACGGA ACTTCAAGAA 
CGAGTGCAGA AGCTTGAGGA TGTGGTGAAG GAGAAAGAAG ATAAGGAAAT TGCTCTTCGT 
AGGGATTTAG AAGACAGCCA TGAGAAATCA AGAGATCTTG ATGACAAGCT TCGAAAAATG 
GAGTTGACAG ATGAGGAGAA AGAGGAAGAT CGGAAGAAGG AACAGAAGAC ATTGAATGAA 
GAGAGGATGA AACTCATGGA ACAGAAAGAA GAAGCAATGC TTGTTGCTAC AAAACATGCA 
ACAACGATTG ATCAACAGAC TCGAAGAATT TCAGTGTTGG AGGGTGACGT GGAAAAGCTG 
ACGGCTGGAA TTGCGGAGAG AGAAAGTTCG ATTAATGCTT TGGAATCGAA TACAATGGAG 
CTGATATCCA AGCTGGAAAC TACTGAAGCA GAACTTGAAA AGCTGAAAGA CGAGCTTGCA 
GTCATGCTGA AACAAAACTC GGAACTCAAA AATGGAAAAG AGGGGTTGAG TGAGAAGTGG 
AATGAGGAGA GGAAGAAGAT CCAAGACTTG GCAGATCAAT TGAGAGAGGC TAACAAAGTT 
GTTCATAATA TGAGAATGAA GAATGTTAAT CTAGAAGAAA AGAAGAATGA ATTGGATCAG 
AATGTGACTG ATTTGACCAA TAAAGTTCGA CAATTAGAGA TTCAGTTGAT GGATAAAGCT 
GCGAAGAACG AGGTTTCAGG AGATTTGCTC AGAAAGATGG AGCATGATGC TCAGAGTATG 
CTGAAGCAAG CACAGAATGA GCAGTTTAGA CTAACTGATC TTGAAAAAGT TCGAAAAGCT 
CTTCAAGATG AGAATCAACG GCTTGTAAAT GATCTGGCAA CAGTGAAAGC TGCATTTGAA 
GTCAAGAGAG AAACGAGCAA ATCTGCAATC AGTGACATTT TGGATAAGTA CCGGAGTGCT 
GAGGAGAAGG CCAATAAGGG TGAATTGGAT AATCAGAGGC TTAGAAGTGA TCTGGCTACT 
GTGACATTAA AACTGGAACG TCAAGAACTG AAAGCAAAAG ATTCTGATAA TCGACTGAGA 
GATAGCCAAA AACGATTCGA AGAAGTTCAA TCAAAACTTG CAAATCTTCA GAAATCAGCA 
GTAGAATCCT TACAAAATCC TATGTCTTCG AATTCCCGCC AAAATCGGTC AATCTATGTT 
GACATTCCAC GTGCAGCTTC TTCGATCGGC CTCAATGAGA ATTCTGATGA AGTCCCGCTT 
CGCAGTTCCC CCTCAGTCCG TTTTGCAGAC AGCTCACAGA ATATGCAAAG AGCAGTTGAC 
TCTATGGATG TGAGTTCTTC AGTTGGAGTT ACACTTCGGT TTTTGAAAGA AAGAATCGAA 
CAGTTGGAAG CAGACAATGC AGATTTATCA GATGCTCTGG AAAAGGCAAA AGATGAGCTG 
AGGCAGAGAA ATGAGAAGCT GGCGGATAGG CAAATGGTTA TTGAGAGAGT TGAGAGACAA 
TTGGTTCATA TTACTGAAGA ACGCAATACA ATTGAAAATC GAATGACAAG TCAGCGTCAA 
ATGTATCTCA CAAATGAGGA ATCATCACGA TCAAGAGAAC ATGAAATCAG AAGTATGAAA 
GCCAGAATTT CAACTTTAGA GCTTCATCTT CGAGAAAAAG AATCTAAACT TGCACATCTA 
CGAAAAGAAA TTGAAGTACT TCATGGTCAG CTTCATGATG CTCTCGAATC AAAGGAAAAG 
GCTACTGGAT TGGTTGGAGT TCAAGATTCA AAGCATCGAG ACTTAGAAGA ACAACTCGAT 
CGAGCAAATC GTGAACGAGA ATTGGCAATT GGGAAGCAGA GAAGAACTCT TGCAGAGAAC 
GAGAATCTGT TCCGGAAGCT TGAACAATTG GAAAAGGAAA GAGAGCAGCT TATGCGAGAG 
ATCACAGATG AACGGAGGCT CAACGAGCGG AATCGCACAA GTCTTGAAGA ACTTCGAGTC 
TCTGAAAGAA CATGGAAATC AGCCATGACA ACTGCCAAAA AACCAGCTGA GGAGCAGGAA 
CGGGCGGTGC AGGAGCAACG GAGATGGGAA GAGTCAAATC ATGAAATGAC GAATAGAAAT 
ACTGCATTGA CAAAAGAATG TGATAGATTA CGAGTTGAAA TGAGAGATCA ACTCAACAGA 
ATGAACGGGA TCAATTTGAG ATCAGTAGAT TTCGAGAGAA AGAATGAAGA GCTTTCAAGT 
AAACTGATTG TTATGCAAAA TACAGTAACT GCGATGAAAA AGTTCGAGGA AGAATGGAAG 
AGGTTGGAGG CGGAGATGAG AGCAGAACTT AAGATTTTGA GAAAAGAAAA GCTCATGCAA 
ACTGCTGAAA TTGAGGATTT AAAGAGGAAA AGCTTCAGAA GTGACACTGA AAAGAAGGAG 
ATCGAGGGTA TTCGAGTAAG GTTGGAAAGG GAAATCAGTG CACTTAAGAG ACATGTTGAT 
GCGCTAGAAG AAGAGAAGGG AAAAACTGAA AAGGCCGTTC GAGAAACCAT GAACGAACGT 
CGTGCCATCG ACAAATCTCT CGCGTCAATG GAACGAGAAA ATCAACAGTT GTATAGGAAT 
TGTGCTCAAC TTCAAGCTCA AATTCAGAAT CTGGAGCGCG ACGCCGGAAA TCGGTCGGTA 
ACCAAGTTGG CTAAGGAGCA TTCATTGCTC GAAGCTCGAA TTGCAGCTTT AATTGAAGAA 
AAACGTCAGC TCCAATCAAT GCTTGACCAA AAAGACGCCA ACTACTCTCA CAAGCGAAAA 
CTCTTAGAAT CCCAAATCCA GTTGCTCCGC GAGCAACTCG AAGCCGAACG CCGAAAGCGT 
ACAAAGGGAG TTGTGGCGAC TGGTCCCACC GTTAGCCGAC GGGGCGTTCA GCACACATCT 
GCCTTTAGGG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_494821.3
CDS	1..5655
Translation	MKSAASSEDVVRVTEDLSECRNRLDAGIEENRRNRQVIQDINDQLQRFRQRANAESIESFNLTPSPDVTLSSLAHPGLTHLHNQTNISMPSLTIDIPLNSNAMISSSRTPNYAINGLRNRHKSLVGHRYRSTSPIGDYGRHRSSPRVLAHYNLDGADIGVGEENLDELFAKLKEELFKNNTLEEVNEMLREENDAALAANEHLRVDATNLSRQLQQLQQQQHTESMRFRSENTRYRNQTETQHRKLISLWKEFTAVKRQLHELRTTTANDLDRQLTEFTRCATLMRKAIRHAEQKNLDQKEQMKREKDDVLDETLRQLNSVTENYMKSEEKANERQRDLKRKEDECRKLREQNDELSDILEQLSKMAHEMAGGRGRNETPMDVARKMRKLLTTKNGEIDESREAAKQAEKERDRAKKDLEKEEKRRKDDREAERKRSSVYSQREHDLKKLDDELRKASEKIRNLEEQRESQEKLTISVQNSLNEAHRQHKQFIEELMIRHREELKEREDSHEEALRSKDTEERSRFEKERSEREKIRRESDELRETQRSLKGDVAAMKTDLDDKTLRLDMLETERDELKKKLETEREQADQRDLEIAECRAKLDEMAEKEAELRKELAEFQAIITAMEGEGKLNQEQFLESKNELNTLTDQIESLNSEVENKNEEIRNLMATLQEKEVHIQNVRTSSHQLTATYEEANGEIDILKAELTRLHEQVNERTRQISEANEKYDDAARKNDALLEDVATWQEKYEQLKMELEEMNRRGQEKEREEADLRALLDDLRGNFDKLTNELKQKGVTVDSLNEEISSLKEQLNKSEKERKEELLRMEELEQKNEAEMKEEYEVKLQLAEKDRQGVENFGKECEARMNELTKIHEMLMEEHDQLKVDHLHTEEEVERLKEKMRKELEKLNEQNDGDRAEWSNERNRLESSKNEAVTELQERVQKLEDVVKEKEDKEIALRRDLEDSHEKSRDLDDKLRKMELTDEEKEEDRKKEQKTLNEERMKLMEQKEEAMLVATKHATTIDQQTRRISVLEGDVEKLTAGIAERESSINALESNTMELISKLETTEAELEKLKDELAVMLKQNSELKNGKEGLSEKWNEERKKIQDLADQLREANKVVHNMRMKNVNLEEKKNELDQNVTDLTNKVRQLEIQLMDKAAKNEVSGDLLRKMEHDAQSMLKQAQNEQFRLTDLEKVRKALQDENQRLVNDLATVKAAFEVKRETSKSAISDILDKYRSAEEKANKGELDNQRLRSDLATVTLKLERQELKAKDSDNRLRDSQKRFEEVQSKLANLQKSAVESLQNPMSSNSRQNRSIYVDIPRAASSIGLNENSDEVPLRSSPSVRFADSSQNMQRAVDSMDVSSSVGVTLRFLKERIEQLEADNADLSDALEKAKDELRQRNEKLADRQMVIERVERQLVHITEERNTIENRMTSQRQMYLTNEESSRSREHEIRSMKARISTLELHLREKESKLAHLRKEIEVLHGQLHDALESKEKATGLVGVQDSKHRDLEEQLDRANRERELAIGKQRRTLAENENLFRKLEQLEKEREQLMREITDERRLNERNRTSLEELRVSERTWKSAMTTAKKPAEEQERAVQEQRRWEESNHEMTNRNTALTKECDRLRVEMRDQLNRMNGINLRSVDFERKNEELSSKLIVMQNTVTAMKKFEEEWKRLEAEMRAELKILRKEKLMQTAEIEDLKRKSFRSDTEKKEIEGIRVRLEREISALKRHVDALEEEKGKTEKAVRETMNERRAIDKSLASMERENQQLYRNCAQLQAQIQNLERDAGNRSVTKLAKEHSLLEARIAALIEEKRQLQSMLDQKDANYSHKRKLLESQIQLLREQLEAERRKRTKGVVATGPTVSRRGVQHTSAFRV

Target ORF information:

RefSeq Version	NM_062420.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Rootletin (che-10), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_062420.3

ORF Insert Sequence:

ATGAAATCGG CGGCAAGTTC GGAGGATGTC GTTCGTGTCA CTGAAGATCT ATCCGAATGC 
CGAAATCGGT TGGATGCTGG GATTGAGGAG AATCGGAGGA ATCGGCAGGT TATCCAGGAT 
ATTAATGATC AGCTCCAACG CTTCCGCCAA AGAGCAAACG CAGAATCAAT CGAGTCTTTC 
AATCTGACCC CCAGCCCCGA TGTGACATTA TCATCACTTG CTCATCCAGG ACTCACACAT 
CTTCACAATC AGACAAACAT TTCCATGCCT TCTCTAACCA TTGACATTCC GTTGAACTCC 
AATGCCATGA TTAGTTCATC TAGAACCCCA AACTATGCGA TTAATGGGTT AAGAAATCGA 
CACAAAAGTC TAGTAGGGCA TAGGTATCGG TCGACGAGCC CGATCGGAGA TTATGGAAGA 
CATCGAAGCA GTCCAAGAGT TTTGGCTCAT TATAATTTGG ATGGTGCAGA TATTGGAGTT 
GGAGAGGAGA ATTTGGACGA ACTTTTTGCA AAGTTGAAAG AGGAATTGTT CAAAAACAAC 
ACACTGGAAG AGGTGAATGA GATGCTCCGA GAAGAAAATG ATGCAGCACT AGCTGCAAAT 
GAGCATTTAA GAGTTGATGC TACTAATTTG AGCAGACAAC TACAGCAATT ACAACAGCAG 
CAGCATACAG AGTCGATGAG ATTTCGGTCG GAGAATACGC GCTATCGAAA TCAAACGGAG 
ACCCAACACA GAAAGCTGAT CAGTCTTTGG AAGGAGTTCA CCGCTGTCAA GCGTCAGCTC 
CACGAGTTGC GCACAACCAC CGCCAACGAT TTGGACAGAC AATTGACCGA ATTCACGCGG 
TGTGCAACAC TTATGCGAAA AGCCATTCGC CACGCCGAGC AAAAGAATCT CGACCAGAAG 
GAACAAATGA AACGAGAAAA GGACGATGTA CTCGATGAGA CACTTCGTCA GCTCAACTCT 
GTCACTGAGA ATTATATGAA ATCAGAAGAA AAAGCAAACG AACGGCAGCG AGATTTGAAG 
AGAAAAGAGG ACGAATGCAG GAAGCTCCGC GAGCAAAACG ACGAGCTCTC GGATATTCTC 
GAACAACTTT CCAAAATGGC TCACGAAATG GCTGGAGGCA GAGGAAGAAA CGAAACACCA 
ATGGATGTGG CACGTAAAAT GCGGAAGCTG CTGACCACCA AGAACGGGGA AATCGATGAA 
TCAAGGGAAG CAGCGAAGCA GGCAGAAAAG GAAAGAGATC GTGCAAAGAA GGATTTGGAA 
AAAGAAGAGA AACGAAGAAA AGATGACAGA GAAGCAGAGA GAAAACGATC AAGTGTCTAT 
TCACAGAGAG AACATGACCT GAAAAAGTTG GATGATGAGT TGAGGAAGGC CAGTGAAAAG 
ATCCGGAATC TCGAGGAGCA ACGGGAGAGC CAGGAAAAAC TCACCATATC AGTTCAGAAC 
TCATTGAATG AAGCACACAG GCAACACAAA CAGTTTATTG AGGAATTGAT GATTCGGCAT 
CGAGAAGAAC TGAAAGAGCG GGAGGATTCT CACGAGGAAG CGTTACGTTC CAAAGATACC 
GAGGAAAGAT CTCGATTTGA AAAAGAACGT TCTGAACGGG AAAAGATTAG ACGAGAAAGT 
GATGAGCTCA GAGAGACGCA GAGAAGTTTA AAGGGTGACG TGGCGGCGAT GAAGACAGAT 
TTGGATGATA AGACTTTAAG GCTTGACATG CTCGAAACAG AACGGGATGA GCTCAAAAAG 
AAACTGGAAA CCGAAAGAGA GCAGGCTGAT CAGAGAGACT TGGAGATAGC AGAGTGTCGA 
GCAAAGCTTG ATGAGATGGC TGAGAAGGAA GCGGAGCTCC GGAAAGAGCT CGCAGAGTTC 
CAGGCAATTA TCACTGCGAT GGAAGGAGAA GGAAAGCTGA ATCAAGAACA GTTTTTGGAG 
AGTAAGAATG AGTTGAACAC GCTGACAGAT CAAATTGAAT CTTTGAATAG TGAGGTGGAG 
AATAAGAATG AGGAAATTCG AAATTTGATG GCTACACTTC AAGAAAAAGA AGTTCACATT 
CAAAACGTCC GAACATCAAG TCATCAGTTG ACTGCCACAT ACGAAGAGGC TAACGGGGAA 
ATCGATATTT TGAAGGCTGA ACTTACCCGG CTCCACGAGC AAGTCAATGA GAGGACTCGA 
CAGATCTCCG AAGCTAATGA GAAGTATGAT GATGCAGCTC GGAAGAATGA CGCATTGCTC 
GAAGATGTCG CCACGTGGCA AGAGAAGTAT GAGCAGTTGA AGATGGAGCT GGAAGAAATG 
AACAGAAGAG GACAGGAGAA GGAACGAGAA GAAGCAGATC TTCGAGCATT ACTTGATGAT 
TTACGTGGAA ACTTTGATAA GTTGACTAAT GAGCTGAAGC AGAAGGGTGT TACTGTTGAT 
AGCTTGAATG AAGAAATTTC ATCGTTGAAA GAACAATTAA ACAAGTCAGA AAAGGAGAGA 
AAAGAAGAAC TGTTAAGGAT GGAAGAGCTT GAACAGAAAA ATGAAGCGGA AATGAAAGAA 
GAGTATGAAG TAAAGTTACA ATTGGCTGAG AAGGATCGTC AAGGAGTTGA GAATTTTGGA 
AAAGAATGTG AAGCGAGAAT GAATGAGCTC ACGAAGATCC ACGAGATGCT AATGGAAGAG 
CACGATCAGT TGAAAGTGGA TCATCTGCAC ACGGAAGAAG AAGTGGAGAG GTTGAAAGAG 
AAAATGAGAA AGGAATTGGA GAAGTTGAAT GAGCAGAATG ATGGTGATCG GGCGGAATGG 
AGTAATGAAA GGAATCGGCT GGAATCATCC AAAAACGAAG CAGTTACGGA ACTTCAAGAA 
CGAGTGCAGA AGCTTGAGGA TGTGGTGAAG GAGAAAGAAG ATAAGGAAAT TGCTCTTCGT 
AGGGATTTAG AAGACAGCCA TGAGAAATCA AGAGATCTTG ATGACAAGCT TCGAAAAATG 
GAGTTGACAG ATGAGGAGAA AGAGGAAGAT CGGAAGAAGG AACAGAAGAC ATTGAATGAA 
GAGAGGATGA AACTCATGGA ACAGAAAGAA GAAGCAATGC TTGTTGCTAC AAAACATGCA 
ACAACGATTG ATCAACAGAC TCGAAGAATT TCAGTGTTGG AGGGTGACGT GGAAAAGCTG 
ACGGCTGGAA TTGCGGAGAG AGAAAGTTCG ATTAATGCTT TGGAATCGAA TACAATGGAG 
CTGATATCCA AGCTGGAAAC TACTGAAGCA GAACTTGAAA AGCTGAAAGA CGAGCTTGCA 
GTCATGCTGA AACAAAACTC GGAACTCAAA AATGGAAAAG AGGGGTTGAG TGAGAAGTGG 
AATGAGGAGA GGAAGAAGAT CCAAGACTTG GCAGATCAAT TGAGAGAGGC TAACAAAGTT 
GTTCATAATA TGAGAATGAA GAATGTTAAT CTAGAAGAAA AGAAGAATGA ATTGGATCAG 
AATGTGACTG ATTTGACCAA TAAAGTTCGA CAATTAGAGA TTCAGTTGAT GGATAAAGCT 
GCGAAGAACG AGGTTTCAGG AGATTTGCTC AGAAAGATGG AGCATGATGC TCAGAGTATG 
CTGAAGCAAG CACAGAATGA GCAGTTTAGA CTAACTGATC TTGAAAAAGT TCGAAAAGCT 
CTTCAAGATG AGAATCAACG GCTTGTAAAT GATCTGGCAA CAGTGAAAGC TGCATTTGAA 
GTCAAGAGAG AAACGAGCAA ATCTGCAATC AGTGACATTT TGGATAAGTA CCGGAGTGCT 
GAGGAGAAGG CCAATAAGGG TGAATTGGAT AATCAGAGGC TTAGAAGTGA TCTGGCTACT 
GTGACATTAA AACTGGAACG TCAAGAACTG AAAGCAAAAG ATTCTGATAA TCGACTGAGA 
GATAGCCAAA AACGATTCGA AGAAGTTCAA TCAAAACTTG CAAATCTTCA GAAATCAGCA 
GTAGAATCCT TACAAAATCC TATGTCTTCG AATTCCCGCC AAAATCGGTC AATCTATGTT 
GACATTCCAC GTGCAGCTTC TTCGATCGGC CTCAATGAGA ATTCTGATGA AGTCCCGCTT 
CGCAGTTCCC CCTCAGTCCG TTTTGCAGAC AGCTCACAGA ATATGCAAAG AGCAGTTGAC 
TCTATGGATG TGAGTTCTTC AGTTGGAGTT ACACTTCGGT TTTTGAAAGA AAGAATCGAA 
CAGTTGGAAG CAGACAATGC AGATTTATCA GATGCTCTGG AAAAGGCAAA AGATGAGCTG 
AGGCAGAGAA ATGAGAAGCT GGCGGATAGG CAAATGGTTA TTGAGAGAGT TGAGAGACAA 
TTGGTTCATA TTACTGAAGA ACGCAATACA ATTGAAAATC GAATGACAAG TCAGCGTCAA 
ATGTATCTCA CAAATGAGGA ATCATCACGA TCAAGAGAAC ATGAAATCAG AAGTATGAAA 
GCCAGAATTT CAACTTTAGA GCTTCATCTT CGAGAAAAAG AATCTAAACT TGCACATCTA 
CGAAAAGAAA TTGAAGTACT TCATGGTCAG CTTCATGATG CTCTCGAATC AAAGGAAAAG 
GCTACTGGAT TGGTTGGAGT TCAAGATTCA AAGCATCGAG ACTTAGAAGA ACAACTCGAT 
CGAGCAAATC GTGAACGAGA ATTGGCAATT GGGAAGCAGA GAAGAACTCT TGCAGAGAAC 
GAGAATCTGT TCCGGAAGCT TGAACAATTG GAAAAGGAAA GAGAGCAGCT TATGCGAGAG 
ATCACAGATG AACGGAGGCT CAACGAGCGG AATCGCACAA GTCTTGAAGA ACTTCGAGTC 
TCTGAAAGAA CATGGAAATC AGCCATGACA ACTGCCAAAA AACCAGCTGA GGAGCAGGAA 
CGGGCGGTGC AGGAGCAACG GAGATGGGAA GAGTCAAATC ATGAAATGAC GAATAGAAAT 
ACTGCATTGA CAAAAGAATG TGATAGATTA CGAGTTGAAA TGAGAGATCA ACTCAACAGA 
ATGAACGGGA TCAATTTGAG ATCAGTAGAT TTCGAGAGAA AGAATGAAGA GCTTTCAAGT 
AAACTGATTG TTATGCAAAA TACAGTAACT GCGATGAAAA AGTTCGAGGA AGAATGGAAG 
AGGTTGGAGG CGGAGATGAG AGCAGAACTT AAGATTTTGA GAAAAGAAAA GCTCATGCAA 
ACTGCTGAAA TTGAGGATTT AAAGAGGAAA AGCTTCAGAA GTGACACTGA AAAGAAGGAG 
ATCGAGGGTA TTCGAGTAAG GTTGGAAAGG GAAATCAGTG CACTTAAGAG ACATGTTGAT 
GCGCTAGAAG AAGAGAAGGG AAAAACTGAA AAGGCCGTTC GAGAAACCAT GAACGAACGT 
CGTGCCATCG ACAAATCTCT CGCGTCAATG GAACGAGAAA ATCAACAGTT GTATAGGAAT 
TGTGCTCAAC TTCAAGCTCA AATTCAGAAT CTGGAGCGCG ACGCCGGAAA TCGGTCGGTA 
ACCAAGTTGG CTAAGGAGCA TTCATTGCTC GAAGCTCGAA TTGCAGCTTT AATTGAAGAA 
AAACGTCAGC TCCAATCAAT GCTTGACCAA AAAGACGCCA ACTACTCTCA CAAGCGAAAA 
CTCTTAGAAT CCCAAATCCA GTTGCTCCGC GAGCAACTCG AAGCCGAACG CCGAAAGCGT 
ACAAAGGGAG TTGTGGCGAC TGGTCCCACC GTTAGCCGAC GGGGCGTTCA GCACACATCT 
GCCTTTAGGG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

che-10 ( NM_062418.7 ) cDNA ORF clone, Caenorhabditis elegans -> NP_494819.3 Caenorhabditis elegans Rootletin (che-10), mRNA.

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CloneID	OCa339203
Clone ID Related Accession (Same CDS sequence)	NM_062418.7 , NM_062418.6
Accession Version	NM_062418.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Rootletin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_062418.6.

ATGAAATCGG CGGCAAGTTC GGAGGATGTC GTTCGTGTCA CTGAAGATCT ATCCGAATGC 
CGAAATCGGT TGGATGCTGG GATTGAGGAG AATCGGAGGA ATCGGCAGGT TATCCAGGAT 
ATTAATGATC AGCTCCAACG CTTCCGCCAA AGAGCAAACG CAGAATCAAT CGAGTCTTTC 
AATCTGACCC CCAGCCCCGA TGTGACATTA TCATCACTTG CTCATCCAGG ACTCACACAT 
CTTCACAATC AGACAAACAT TTCCATGCCT TCTCTAACCA TTGACATTCC GTTGAACTCC 
AATGCCATGA TTAGTTCATC TAGAACCCCA AACTATGCGA TTAATGGGTT AAGAAATCGA 
CACAAAAGTC TAGTAGGGCA TAGGTATCGG TCGACGAGCC CGATCGGAGA TTATGGAAGA 
CATCGAAGCA GTCCAAGAGT TTTGGCTCAT TATAATTTGG ATGGTGCAGA TATTGGAGTT 
GGAGAGGAGA ATTTGGACGA ACTTTTTGCA AAGTTGAAAG AGGAATTGTT CAAAAACAAC 
ACACTGGAAG AGGTGAATGA GATGCTCCGA GAAGAAAATG ATGCAGCACT AGCTGCAAAT 
GAGCATTTAA GAGTTGATGC TACTAATTTG AGCAGACAAC TACAGCAATT ACAACAGCAG 
CAGCATACAG AGTCGATGAG ATTTCGGTCG GAGAATACGC GCTATCGAAA TCAAACGGAG 
ACCCAACACA GAAAGCTGAT CAGTCTTTGG AAGGAGTTCA CCGCTGTCAA GCGTCAGCTC 
CACGAGTTGC GCACAACCAC CGCCAACGAT TTGGACAGAC AATTGACCGA ATTCACGCGG 
TGTGCAACAC TTATGCGAAA AGCCATTCGC CACGCCGAGC AAAAGAATCT CGACCAGAAG 
GAACAAATGA AACGAGAAAA GGACGATGTA CTCGATGAGA CACTTCGTCA GCTCAACTCT 
GTCACTGAGA ATTATATGAA ATCAGAAGAA AAAGCAAACG AACGGCAGCG AGATTTGAAG 
AGAAAAGAGG ACGAATGCAG GAAGCTCCGC GAGCAAAACG ACGAGCTCTC GGATATTCTC 
GAACAACTTT CCAAAATGGC TCACGAAATG GCTGGAGGCA GAGGAAGAAA CGAAACACCA 
ATGGATGTGG CACGTAAAAT GCGGAAGCTG CTGACCACCA AGAACGGGGA AATCGATGAA 
TCAAGGGAAG CAGCGAAGCA GGCAGAAAAG GAAAGAGATC GTGCAAAGAA GGATTTGGAA 
AAAGAAGAGA AACGAAGAAA AGATGACAGA GAAGCAGAGA GAAAACGATC AAGTGTCTAT 
TCACAGAGAG AACATGACCT GAAAAAGTTG GATGATGAGT TGAGGAAGGC CAGTGAAAAG 
ATCCGGAATC TCGAGGAGCA ACGGGAGAGC CAGGAAAAAC TCACCATATC AGTTCAGAAC 
TCATTGAATG AAGCACACAG GCAACACAAA CAGTTTATTG AGGAATTGAT GATTCGGCAT 
CGAGAAGAAC TGAAAGAGCG GGAGGATTCT CACGAGGAAG CGTTACGTTC CAAAGATACC 
GAGGAAAGAT CTCGATTTGA AAAAGAACGT TCTGAACGGG AAAAGATTAG ACGAGAAAGT 
GATGAGCTCA GAGAGACGCA GAGAAGTTTA AAGGGTGACG TGGCGGCGAT GAAGACAGAT 
TTGGATGATA AGACTTTAAG GCTTGACATG CTCGAAACAG AACGGGATGA GCTCAAAAAG 
AAACTGGAAA CCGAAAGAGA GCAGGCTGAT CAGAGAGACT TGGAGATAGC AGAGTGTCGA 
GCAAAGCTTG ATGAGATGGC TGAGAAGGAA GCGGAGCTCC GGAAAGAGCT CGCAGAGTTC 
CAGGCAATTA TCACTGCGAT GGAAGGAGAA GGAAAGCTGA ATCAAGAACA GTTTTTGGAG 
AGTAAGAATG AGTTGAACAC GCTGACAGAT CAAATTGAAT CTTTGAATAG TGAGGTGGAG 
AATAAGAATG AGGAAATTCG AAATTTGATG GCTACACTTC AAGAAAAAGA AGTTCACATT 
CAAAACGTCC GAACATCAAG TCATCAGTTG ACTGCCACAT ACGAAGAGGC TAACGGGGAA 
ATCGATATTT TGAAGGCTGA ACTTACCCGG CTCCACGAGC AAGTCAATGA GAGGACTCGA 
CAGATCTCCG AAGCTAATGA GAAGTATGAT GATGCAGCTC GGAAGAATGA CGCATTGCTC 
GAAGATGTCG CCACGTGGCA AGAGAAGTAT GAGCAGTTGA AGATGGAGCT GGAAGAAATG 
AACAGAAGAG GACAGGAGAA GGAACGAGAA GAAGCAGATC TTCGAGCATT ACTTGATGAT 
TTACGTGGAA ACTTTGATAA GTTGACTAAT GAGCTGAAGC AGAAGGGTGT TACTGTTGAT 
AGCTTGAATG AAGAAATTTC ATCGTTGAAA GAACAATTAA ACAAGTCAGA AAAGGAGAGA 
AAAGAAGAAC TGTTAAGGAT GGAAGAGCTT GAACAGAAAA ATGAAGCGGA AATGAAAGAA 
GAGTATGAAG TAAAGTTACA ATTGGCTGAG AAGGATCGTC AAGGAGTTGA GAATTTTGGA 
AAAGAATGTG AAGCGAGAAT GAATGAGCTC ACGAAGATCC ACGAGATGCT AATGGAAGAG 
CACGATCAGT TGAAAGTGGA TCATCTGCAC ACGGAAGAAG AAGTGGAGAG GTTGAAAGAG 
AAAATGAGAA AGGAATTGGA GAAGTTGAAT GAGCAGAATG ATGGTGATCG GGCGGAATGG 
AGTAATGAAA GGAATCGGCT GGAATCATCC AAAAACGAAG CAGTTACGGA ACTTCAAGAA 
CGAGTGCAGA AGCTTGAGGA TGTGGTGAAG GAGAAAGAAG ATAAGGAAAT TGCTCTTCGT 
AGGGATTTAG AAGACAGCCA TGAGAAATCA AGAGATCTTG ATGACAAGCT TCGAAAAATG 
GAGTTGACAG ATGAGGAGAA AGAGGAAGAT CGGAAGAAGG AACAGAAGAC ATTGAATGAA 
GAGAGGATGA AACTCATGGA ACAGAAAGAA GAAGCAATGC TTGTTGCTAC AAAACATGCA 
ACAACGATTG ATCAACAGAC TCGAAGAATT TCAGTGTTGG AGGGTGACGT GGAAAAGCTG 
ACGGCTGGAA TTGCGGAGAG AGAAAGTTCG ATTAATGCTT TGGAATCGAA TACAATGGAG 
CTGATATCCA AGCTGGAAAC TACTGAAGCA GAACTTGAAA AGCTGAAAGA CGAGCTTGCA 
GTCATGCTGA AACAAAACTC GGAACTCAAA AATGGAAAAG AGGGGTTGAG TGAGAAGTGG 
AATGAGGAGA GGAAGAAGAT CCAAGACTTG GCAGATCAAT TGAGAGAGGC TAACAAAGTT 
GTTCATAATA TGAGAATGAA GAATGTTAAT CTAGAAGAAA AGAAGAATGA ATTGGATCAG 
AATGTGACTG ATTTGACCAA TAAAGTTCGA CAATTAGAGA TTCAGTTGAT GGATAAAGCT 
GCGAAGAACG AGGTTTCAGG AGATTTGCTC AGAAAGATGG AGCATGATGC TCAGAGTATG 
CTGAAGCAAG CACAGAATGA GCAGTTTAGA CTAACTGATC TTGAAAAAGT TCGAAAAGCT 
CTTCAAGATG AGAATCAACG GCTTGTAAAT GATCTGGCAA CAGTGAAAGC TGCATTTGAA 
GTCAAGAGAG AAACGAGCAA ATCTGCAATC AGTGACATTT TGGATAAGTA CCGGAGTGCT 
GAGGAGAAGG CCAATAAGGG TGAATTGGAT AATCAGAGGC TTAGAAGTGA TCTGGCTACT 
GTGACATTAA AACTGGAACG TCAAGAACTG AAAGCAAAAG ATTCTGATAA TCGACTGAGA 
GATAGCCAAA AACGATTCGA AGAAGTTCAA TCAAAACTTG CAAATCTTCA GAAATCAGCA 
GTAGAATCCT TACAAAATCC TATGTCTTCG AATTCCCGCC AAAATCGGTC AATCTATGTT 
GACATTCCAC GTGCAGCTTC TTCGATCGGC CTCAATGAGA ATTCTGATGA AGTCCCGCTT 
CGCAGTTCCC CCTCAGTCCG TTTTGCAGAC AGCTCACAGA ATATGCAAAG AGCAGTTGAC 
TCTATGGATG TGAGTTCTTC AGTTGGAGTT ACACTTCGGT TTTTGAAAGA AAGAATCGAA 
CAGTTGGAAG CAGACAATGC AGATTTATCA GATGCTCTGG AAAAGGCAAA AGATGAGCTG 
AGGCAGAGAA ATGAGAAGCT GGCGGATAGG CAAATGGTTA TTGAGAGAGT TGAGAGACAA 
TTGGTTCATA TTACTGAAGA ACGCAATACA ATTGAAAATC GAATGACAAG TCAGCGTCAA 
ATGTATCTCA CAAATGAGGA ATCATCACGA TCAAGAGAAC ATGAAATCAG AAGTATGAAA 
GCCAGAATTT CAACTTTAGA GCTTCATCTT CGAGAAAAAG AATCTAAACT TGCACATCTA 
CGAAAAGAAA TTGAAGTACT TCATGGTCAG CTTCATGATG CTCTCGAATC AAAGGAAAAG 
GCTACTGGAT TGGTTGGAGT TCAAGATTCA AAGCATCGAG ACTTAGAAGA ACAACTCGAT 
CGAGCAAATC GTGAACGAGA ATTGGCAATT GGGAAGCAGA GAAGAACTCT TGCAGAGAAC 
GAGAATCTGT TCCGGAAGCT TGAACAATTG GAAAAGGAAA GAGAGCAGCT TATGCGAGAG 
ATCACAGATG AACGGAGGCT CAACGAGCGG AATCGCACAA GTCTTGAAGA ACTTCGAGTC 
TCTGAAAGAA CATGGAAATC AGCCATGACA ACTGCCAAAA AACCAGCTGA GGAGCAGGAA 
CGGGCGGTGC AGGAGCAACG GAGATGGGAA GAGTCAAATC ATGAAATGAC GAATAGAAAT 
ACTGCATTGA CAAAAGAATG TGATAGATTA CGAGTTGAAA TGAGAGATCA ACTCAACAGA 
ATGAACGGGA TCAATTTGAG ATCAGTAGAT TTCGAGAGAA AGAATGAAGA GCTTTCAAGT 
AAACTGATTG TTATGCAAAA TACAGTAACT GCGATGAAAA AGTTCGAGGA AGAATGGAAG 
AGGTTGGAGG CGGAGATGAG AGCAGAACTT AAGATTTTGA GAAAAGAAAA GCTCATGCAA 
ACTGCTGAAA TTGAGGATTT AAAGAGGAAA AGCTTCAGAA GTGACACTGA AAAGAAGGAG 
ATCGAGGGTA TTCGAGTAAG GTTGGAAAGG GAAATCAGTG CACTTAAGAG ACATGTTGAT 
GCGCTAGAAG AAGAGAAGGG AAAAACTGAA AAGGCCGTTC GAGAAACCAT GAACGAACGT 
CGTGCCATCG ACAAATCTCT CGCGTCAATG GAACGAGAAA ATCAACAGTT GTATAGGAAT 
TGTGCTCAAC TTCAAGCTCA AATTCAGAAT CTGGAGCGCG ACGCCGGAAA TCGGTCGGTA 
ACCAAGTTGG CTAAGGAGCA TTCATTGCTC GAAGCTCGAA TTGCAGCTTT AATTGAAGAA 
AAACGTCAGC TCCAATCAAT GCTTGACCAA AAAGACGCCA ACTACTCTCA CAAGCGAAAA 
CTCTTAGAAT CCCAAATCCA GTTGCTCCGC GAGCAACTCG AAGCCGAACG CCGAAAGCGT 
ACAAAGGGAG TTGTGGCGAC TGGTCCCACC GTTAGCCGAC GGGGCGTTCA GCACACATCT 
GCCTTTAGGC ACACCATCGA GCGTCATCGG TCTCTGTCCC AGTCTAGTGA ACGGACAATT 
CTTCAGGAAC GATACCTTGA ATATGTATAT ACAGGAGATC GTACTCCAGC AATACAAATG 
ATCAACACAC CGCCAATCTC TCCATTATCA CATTCTGGCT CTTTCAATTC GGACGGAAGG 
GAAGCCAGAA TCCGGAGAGA GTCTGACAAC GGCCATCTTC AGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_494819.3
CDS	44..5911
Translation	MKSAASSEDVVRVTEDLSECRNRLDAGIEENRRNRQVIQDINDQLQRFRQRANAESIESFNLTPSPDVTLSSLAHPGLTHLHNQTNISMPSLTIDIPLNSNAMISSSRTPNYAINGLRNRHKSLVGHRYRSTSPIGDYGRHRSSPRVLAHYNLDGADIGVGEENLDELFAKLKEELFKNNTLEEVNEMLREENDAALAANEHLRVDATNLSRQLQQLQQQQHTESMRFRSENTRYRNQTETQHRKLISLWKEFTAVKRQLHELRTTTANDLDRQLTEFTRCATLMRKAIRHAEQKNLDQKEQMKREKDDVLDETLRQLNSVTENYMKSEEKANERQRDLKRKEDECRKLREQNDELSDILEQLSKMAHEMAGGRGRNETPMDVARKMRKLLTTKNGEIDESREAAKQAEKERDRAKKDLEKEEKRRKDDREAERKRSSVYSQREHDLKKLDDELRKASEKIRNLEEQRESQEKLTISVQNSLNEAHRQHKQFIEELMIRHREELKEREDSHEEALRSKDTEERSRFEKERSEREKIRRESDELRETQRSLKGDVAAMKTDLDDKTLRLDMLETERDELKKKLETEREQADQRDLEIAECRAKLDEMAEKEAELRKELAEFQAIITAMEGEGKLNQEQFLESKNELNTLTDQIESLNSEVENKNEEIRNLMATLQEKEVHIQNVRTSSHQLTATYEEANGEIDILKAELTRLHEQVNERTRQISEANEKYDDAARKNDALLEDVATWQEKYEQLKMELEEMNRRGQEKEREEADLRALLDDLRGNFDKLTNELKQKGVTVDSLNEEISSLKEQLNKSEKERKEELLRMEELEQKNEAEMKEEYEVKLQLAEKDRQGVENFGKECEARMNELTKIHEMLMEEHDQLKVDHLHTEEEVERLKEKMRKELEKLNEQNDGDRAEWSNERNRLESSKNEAVTELQERVQKLEDVVKEKEDKEIALRRDLEDSHEKSRDLDDKLRKMELTDEEKEEDRKKEQKTLNEERMKLMEQKEEAMLVATKHATTIDQQTRRISVLEGDVEKLTAGIAERESSINALESNTMELISKLETTEAELEKLKDELAVMLKQNSELKNGKEGLSEKWNEERKKIQDLADQLREANKVVHNMRMKNVNLEEKKNELDQNVTDLTNKVRQLEIQLMDKAAKNEVSGDLLRKMEHDAQSMLKQAQNEQFRLTDLEKVRKALQDENQRLVNDLATVKAAFEVKRETSKSAISDILDKYRSAEEKANKGELDNQRLRSDLATVTLKLERQELKAKDSDNRLRDSQKRFEEVQSKLANLQKSAVESLQNPMSSNSRQNRSIYVDIPRAASSIGLNENSDEVPLRSSPSVRFADSSQNMQRAVDSMDVSSSVGVTLRFLKERIEQLEADNADLSDALEKAKDELRQRNEKLADRQMVIERVERQLVHITEERNTIENRMTSQRQMYLTNEESSRSREHEIRSMKARISTLELHLREKESKLAHLRKEIEVLHGQLHDALESKEKATGLVGVQDSKHRDLEEQLDRANRERELAIGKQRRTLAENENLFRKLEQLEKEREQLMREITDERRLNERNRTSLEELRVSERTWKSAMTTAKKPAEEQERAVQEQRRWEESNHEMTNRNTALTKECDRLRVEMRDQLNRMNGINLRSVDFERKNEELSSKLIVMQNTVTAMKKFEEEWKRLEAEMRAELKILRKEKLMQTAEIEDLKRKSFRSDTEKKEIEGIRVRLEREISALKRHVDALEEEKGKTEKAVRETMNERRAIDKSLASMERENQQLYRNCAQLQAQIQNLERDAGNRSVTKLAKEHSLLEARIAALIEEKRQLQSMLDQKDANYSHKRKLLESQIQLLREQLEAERRKRTKGVVATGPTVSRRGVQHTSAFRHTIERHRSLSQSSERTILQERYLEYVYTGDRTPAIQMINTPPISPLSHSGSFNSDGREARIRRESDNGHLQH

Target ORF information:

RefSeq Version	NM_062418.7
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Rootletin (che-10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_062418.7

ORF Insert Sequence:

ATGAAATCGG CGGCAAGTTC GGAGGATGTC GTTCGTGTCA CTGAAGATCT ATCCGAATGC 
CGAAATCGGT TGGATGCTGG GATTGAGGAG AATCGGAGGA ATCGGCAGGT TATCCAGGAT 
ATTAATGATC AGCTCCAACG CTTCCGCCAA AGAGCAAACG CAGAATCAAT CGAGTCTTTC 
AATCTGACCC CCAGCCCCGA TGTGACATTA TCATCACTTG CTCATCCAGG ACTCACACAT 
CTTCACAATC AGACAAACAT TTCCATGCCT TCTCTAACCA TTGACATTCC GTTGAACTCC 
AATGCCATGA TTAGTTCATC TAGAACCCCA AACTATGCGA TTAATGGGTT AAGAAATCGA 
CACAAAAGTC TAGTAGGGCA TAGGTATCGG TCGACGAGCC CGATCGGAGA TTATGGAAGA 
CATCGAAGCA GTCCAAGAGT TTTGGCTCAT TATAATTTGG ATGGTGCAGA TATTGGAGTT 
GGAGAGGAGA ATTTGGACGA ACTTTTTGCA AAGTTGAAAG AGGAATTGTT CAAAAACAAC 
ACACTGGAAG AGGTGAATGA GATGCTCCGA GAAGAAAATG ATGCAGCACT AGCTGCAAAT 
GAGCATTTAA GAGTTGATGC TACTAATTTG AGCAGACAAC TACAGCAATT ACAACAGCAG 
CAGCATACAG AGTCGATGAG ATTTCGGTCG GAGAATACGC GCTATCGAAA TCAAACGGAG 
ACCCAACACA GAAAGCTGAT CAGTCTTTGG AAGGAGTTCA CCGCTGTCAA GCGTCAGCTC 
CACGAGTTGC GCACAACCAC CGCCAACGAT TTGGACAGAC AATTGACCGA ATTCACGCGG 
TGTGCAACAC TTATGCGAAA AGCCATTCGC CACGCCGAGC AAAAGAATCT CGACCAGAAG 
GAACAAATGA AACGAGAAAA GGACGATGTA CTCGATGAGA CACTTCGTCA GCTCAACTCT 
GTCACTGAGA ATTATATGAA ATCAGAAGAA AAAGCAAACG AACGGCAGCG AGATTTGAAG 
AGAAAAGAGG ACGAATGCAG GAAGCTCCGC GAGCAAAACG ACGAGCTCTC GGATATTCTC 
GAACAACTTT CCAAAATGGC TCACGAAATG GCTGGAGGCA GAGGAAGAAA CGAAACACCA 
ATGGATGTGG CACGTAAAAT GCGGAAGCTG CTGACCACCA AGAACGGGGA AATCGATGAA 
TCAAGGGAAG CAGCGAAGCA GGCAGAAAAG GAAAGAGATC GTGCAAAGAA GGATTTGGAA 
AAAGAAGAGA AACGAAGAAA AGATGACAGA GAAGCAGAGA GAAAACGATC AAGTGTCTAT 
TCACAGAGAG AACATGACCT GAAAAAGTTG GATGATGAGT TGAGGAAGGC CAGTGAAAAG 
ATCCGGAATC TCGAGGAGCA ACGGGAGAGC CAGGAAAAAC TCACCATATC AGTTCAGAAC 
TCATTGAATG AAGCACACAG GCAACACAAA CAGTTTATTG AGGAATTGAT GATTCGGCAT 
CGAGAAGAAC TGAAAGAGCG GGAGGATTCT CACGAGGAAG CGTTACGTTC CAAAGATACC 
GAGGAAAGAT CTCGATTTGA AAAAGAACGT TCTGAACGGG AAAAGATTAG ACGAGAAAGT 
GATGAGCTCA GAGAGACGCA GAGAAGTTTA AAGGGTGACG TGGCGGCGAT GAAGACAGAT 
TTGGATGATA AGACTTTAAG GCTTGACATG CTCGAAACAG AACGGGATGA GCTCAAAAAG 
AAACTGGAAA CCGAAAGAGA GCAGGCTGAT CAGAGAGACT TGGAGATAGC AGAGTGTCGA 
GCAAAGCTTG ATGAGATGGC TGAGAAGGAA GCGGAGCTCC GGAAAGAGCT CGCAGAGTTC 
CAGGCAATTA TCACTGCGAT GGAAGGAGAA GGAAAGCTGA ATCAAGAACA GTTTTTGGAG 
AGTAAGAATG AGTTGAACAC GCTGACAGAT CAAATTGAAT CTTTGAATAG TGAGGTGGAG 
AATAAGAATG AGGAAATTCG AAATTTGATG GCTACACTTC AAGAAAAAGA AGTTCACATT 
CAAAACGTCC GAACATCAAG TCATCAGTTG ACTGCCACAT ACGAAGAGGC TAACGGGGAA 
ATCGATATTT TGAAGGCTGA ACTTACCCGG CTCCACGAGC AAGTCAATGA GAGGACTCGA 
CAGATCTCCG AAGCTAATGA GAAGTATGAT GATGCAGCTC GGAAGAATGA CGCATTGCTC 
GAAGATGTCG CCACGTGGCA AGAGAAGTAT GAGCAGTTGA AGATGGAGCT GGAAGAAATG 
AACAGAAGAG GACAGGAGAA GGAACGAGAA GAAGCAGATC TTCGAGCATT ACTTGATGAT 
TTACGTGGAA ACTTTGATAA GTTGACTAAT GAGCTGAAGC AGAAGGGTGT TACTGTTGAT 
AGCTTGAATG AAGAAATTTC ATCGTTGAAA GAACAATTAA ACAAGTCAGA AAAGGAGAGA 
AAAGAAGAAC TGTTAAGGAT GGAAGAGCTT GAACAGAAAA ATGAAGCGGA AATGAAAGAA 
GAGTATGAAG TAAAGTTACA ATTGGCTGAG AAGGATCGTC AAGGAGTTGA GAATTTTGGA 
AAAGAATGTG AAGCGAGAAT GAATGAGCTC ACGAAGATCC ACGAGATGCT AATGGAAGAG 
CACGATCAGT TGAAAGTGGA TCATCTGCAC ACGGAAGAAG AAGTGGAGAG GTTGAAAGAG 
AAAATGAGAA AGGAATTGGA GAAGTTGAAT GAGCAGAATG ATGGTGATCG GGCGGAATGG 
AGTAATGAAA GGAATCGGCT GGAATCATCC AAAAACGAAG CAGTTACGGA ACTTCAAGAA 
CGAGTGCAGA AGCTTGAGGA TGTGGTGAAG GAGAAAGAAG ATAAGGAAAT TGCTCTTCGT 
AGGGATTTAG AAGACAGCCA TGAGAAATCA AGAGATCTTG ATGACAAGCT TCGAAAAATG 
GAGTTGACAG ATGAGGAGAA AGAGGAAGAT CGGAAGAAGG AACAGAAGAC ATTGAATGAA 
GAGAGGATGA AACTCATGGA ACAGAAAGAA GAAGCAATGC TTGTTGCTAC AAAACATGCA 
ACAACGATTG ATCAACAGAC TCGAAGAATT TCAGTGTTGG AGGGTGACGT GGAAAAGCTG 
ACGGCTGGAA TTGCGGAGAG AGAAAGTTCG ATTAATGCTT TGGAATCGAA TACAATGGAG 
CTGATATCCA AGCTGGAAAC TACTGAAGCA GAACTTGAAA AGCTGAAAGA CGAGCTTGCA 
GTCATGCTGA AACAAAACTC GGAACTCAAA AATGGAAAAG AGGGGTTGAG TGAGAAGTGG 
AATGAGGAGA GGAAGAAGAT CCAAGACTTG GCAGATCAAT TGAGAGAGGC TAACAAAGTT 
GTTCATAATA TGAGAATGAA GAATGTTAAT CTAGAAGAAA AGAAGAATGA ATTGGATCAG 
AATGTGACTG ATTTGACCAA TAAAGTTCGA CAATTAGAGA TTCAGTTGAT GGATAAAGCT 
GCGAAGAACG AGGTTTCAGG AGATTTGCTC AGAAAGATGG AGCATGATGC TCAGAGTATG 
CTGAAGCAAG CACAGAATGA GCAGTTTAGA CTAACTGATC TTGAAAAAGT TCGAAAAGCT 
CTTCAAGATG AGAATCAACG GCTTGTAAAT GATCTGGCAA CAGTGAAAGC TGCATTTGAA 
GTCAAGAGAG AAACGAGCAA ATCTGCAATC AGTGACATTT TGGATAAGTA CCGGAGTGCT 
GAGGAGAAGG CCAATAAGGG TGAATTGGAT AATCAGAGGC TTAGAAGTGA TCTGGCTACT 
GTGACATTAA AACTGGAACG TCAAGAACTG AAAGCAAAAG ATTCTGATAA TCGACTGAGA 
GATAGCCAAA AACGATTCGA AGAAGTTCAA TCAAAACTTG CAAATCTTCA GAAATCAGCA 
GTAGAATCCT TACAAAATCC TATGTCTTCG AATTCCCGCC AAAATCGGTC AATCTATGTT 
GACATTCCAC GTGCAGCTTC TTCGATCGGC CTCAATGAGA ATTCTGATGA AGTCCCGCTT 
CGCAGTTCCC CCTCAGTCCG TTTTGCAGAC AGCTCACAGA ATATGCAAAG AGCAGTTGAC 
TCTATGGATG TGAGTTCTTC AGTTGGAGTT ACACTTCGGT TTTTGAAAGA AAGAATCGAA 
CAGTTGGAAG CAGACAATGC AGATTTATCA GATGCTCTGG AAAAGGCAAA AGATGAGCTG 
AGGCAGAGAA ATGAGAAGCT GGCGGATAGG CAAATGGTTA TTGAGAGAGT TGAGAGACAA 
TTGGTTCATA TTACTGAAGA ACGCAATACA ATTGAAAATC GAATGACAAG TCAGCGTCAA 
ATGTATCTCA CAAATGAGGA ATCATCACGA TCAAGAGAAC ATGAAATCAG AAGTATGAAA 
GCCAGAATTT CAACTTTAGA GCTTCATCTT CGAGAAAAAG AATCTAAACT TGCACATCTA 
CGAAAAGAAA TTGAAGTACT TCATGGTCAG CTTCATGATG CTCTCGAATC AAAGGAAAAG 
GCTACTGGAT TGGTTGGAGT TCAAGATTCA AAGCATCGAG ACTTAGAAGA ACAACTCGAT 
CGAGCAAATC GTGAACGAGA ATTGGCAATT GGGAAGCAGA GAAGAACTCT TGCAGAGAAC 
GAGAATCTGT TCCGGAAGCT TGAACAATTG GAAAAGGAAA GAGAGCAGCT TATGCGAGAG 
ATCACAGATG AACGGAGGCT CAACGAGCGG AATCGCACAA GTCTTGAAGA ACTTCGAGTC 
TCTGAAAGAA CATGGAAATC AGCCATGACA ACTGCCAAAA AACCAGCTGA GGAGCAGGAA 
CGGGCGGTGC AGGAGCAACG GAGATGGGAA GAGTCAAATC ATGAAATGAC GAATAGAAAT 
ACTGCATTGA CAAAAGAATG TGATAGATTA CGAGTTGAAA TGAGAGATCA ACTCAACAGA 
ATGAACGGGA TCAATTTGAG ATCAGTAGAT TTCGAGAGAA AGAATGAAGA GCTTTCAAGT 
AAACTGATTG TTATGCAAAA TACAGTAACT GCGATGAAAA AGTTCGAGGA AGAATGGAAG 
AGGTTGGAGG CGGAGATGAG AGCAGAACTT AAGATTTTGA GAAAAGAAAA GCTCATGCAA 
ACTGCTGAAA TTGAGGATTT AAAGAGGAAA AGCTTCAGAA GTGACACTGA AAAGAAGGAG 
ATCGAGGGTA TTCGAGTAAG GTTGGAAAGG GAAATCAGTG CACTTAAGAG ACATGTTGAT 
GCGCTAGAAG AAGAGAAGGG AAAAACTGAA AAGGCCGTTC GAGAAACCAT GAACGAACGT 
CGTGCCATCG ACAAATCTCT CGCGTCAATG GAACGAGAAA ATCAACAGTT GTATAGGAAT 
TGTGCTCAAC TTCAAGCTCA AATTCAGAAT CTGGAGCGCG ACGCCGGAAA TCGGTCGGTA 
ACCAAGTTGG CTAAGGAGCA TTCATTGCTC GAAGCTCGAA TTGCAGCTTT AATTGAAGAA 
AAACGTCAGC TCCAATCAAT GCTTGACCAA AAAGACGCCA ACTACTCTCA CAAGCGAAAA 
CTCTTAGAAT CCCAAATCCA GTTGCTCCGC GAGCAACTCG AAGCCGAACG CCGAAAGCGT 
ACAAAGGGAG TTGTGGCGAC TGGTCCCACC GTTAGCCGAC GGGGCGTTCA GCACACATCT 
GCCTTTAGGC ACACCATCGA GCGTCATCGG TCTCTGTCCC AGTCTAGTGA ACGGACAATT 
CTTCAGGAAC GATACCTTGA ATATGTATAT ACAGGAGATC GTACTCCAGC AATACAAATG 
ATCAACACAC CGCCAATCTC TCCATTATCA CATTCTGGCT CTTTCAATTC GGACGGAAGG 
GAAGCCAGAA TCCGGAGAGA GTCTGACAAC GGCCATCTTC AGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

che-10 ( NM_062418.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_494819.3 Caenorhabditis elegans Rootletin (che-10), partial mRNA.

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CloneID	OCa339203
Clone ID Related Accession (Same CDS sequence)	NM_062418.7 , NM_062418.6
Accession Version	NM_062418.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Rootletin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Nov 13, 2008 this sequence version replaced NM_062418.5. COMPLETENESS: incomplete on both ends.

ATGAAATCGG CGGCAAGTTC GGAGGATGTC GTTCGTGTCA CTGAAGATCT ATCCGAATGC 
CGAAATCGGT TGGATGCTGG GATTGAGGAG AATCGGAGGA ATCGGCAGGT TATCCAGGAT 
ATTAATGATC AGCTCCAACG CTTCCGCCAA AGAGCAAACG CAGAATCAAT CGAGTCTTTC 
AATCTGACCC CCAGCCCCGA TGTGACATTA TCATCACTTG CTCATCCAGG ACTCACACAT 
CTTCACAATC AGACAAACAT TTCCATGCCT TCTCTAACCA TTGACATTCC GTTGAACTCC 
AATGCCATGA TTAGTTCATC TAGAACCCCA AACTATGCGA TTAATGGGTT AAGAAATCGA 
CACAAAAGTC TAGTAGGGCA TAGGTATCGG TCGACGAGCC CGATCGGAGA TTATGGAAGA 
CATCGAAGCA GTCCAAGAGT TTTGGCTCAT TATAATTTGG ATGGTGCAGA TATTGGAGTT 
GGAGAGGAGA ATTTGGACGA ACTTTTTGCA AAGTTGAAAG AGGAATTGTT CAAAAACAAC 
ACACTGGAAG AGGTGAATGA GATGCTCCGA GAAGAAAATG ATGCAGCACT AGCTGCAAAT 
GAGCATTTAA GAGTTGATGC TACTAATTTG AGCAGACAAC TACAGCAATT ACAACAGCAG 
CAGCATACAG AGTCGATGAG ATTTCGGTCG GAGAATACGC GCTATCGAAA TCAAACGGAG 
ACCCAACACA GAAAGCTGAT CAGTCTTTGG AAGGAGTTCA CCGCTGTCAA GCGTCAGCTC 
CACGAGTTGC GCACAACCAC CGCCAACGAT TTGGACAGAC AATTGACCGA ATTCACGCGG 
TGTGCAACAC TTATGCGAAA AGCCATTCGC CACGCCGAGC AAAAGAATCT CGACCAGAAG 
GAACAAATGA AACGAGAAAA GGACGATGTA CTCGATGAGA CACTTCGTCA GCTCAACTCT 
GTCACTGAGA ATTATATGAA ATCAGAAGAA AAAGCAAACG AACGGCAGCG AGATTTGAAG 
AGAAAAGAGG ACGAATGCAG GAAGCTCCGC GAGCAAAACG ACGAGCTCTC GGATATTCTC 
GAACAACTTT CCAAAATGGC TCACGAAATG GCTGGAGGCA GAGGAAGAAA CGAAACACCA 
ATGGATGTGG CACGTAAAAT GCGGAAGCTG CTGACCACCA AGAACGGGGA AATCGATGAA 
TCAAGGGAAG CAGCGAAGCA GGCAGAAAAG GAAAGAGATC GTGCAAAGAA GGATTTGGAA 
AAAGAAGAGA AACGAAGAAA AGATGACAGA GAAGCAGAGA GAAAACGATC AAGTGTCTAT 
TCACAGAGAG AACATGACCT GAAAAAGTTG GATGATGAGT TGAGGAAGGC CAGTGAAAAG 
ATCCGGAATC TCGAGGAGCA ACGGGAGAGC CAGGAAAAAC TCACCATATC AGTTCAGAAC 
TCATTGAATG AAGCACACAG GCAACACAAA CAGTTTATTG AGGAATTGAT GATTCGGCAT 
CGAGAAGAAC TGAAAGAGCG GGAGGATTCT CACGAGGAAG CGTTACGTTC CAAAGATACC 
GAGGAAAGAT CTCGATTTGA AAAAGAACGT TCTGAACGGG AAAAGATTAG ACGAGAAAGT 
GATGAGCTCA GAGAGACGCA GAGAAGTTTA AAGGGTGACG TGGCGGCGAT GAAGACAGAT 
TTGGATGATA AGACTTTAAG GCTTGACATG CTCGAAACAG AACGGGATGA GCTCAAAAAG 
AAACTGGAAA CCGAAAGAGA GCAGGCTGAT CAGAGAGACT TGGAGATAGC AGAGTGTCGA 
GCAAAGCTTG ATGAGATGGC TGAGAAGGAA GCGGAGCTCC GGAAAGAGCT CGCAGAGTTC 
CAGGCAATTA TCACTGCGAT GGAAGGAGAA GGAAAGCTGA ATCAAGAACA GTTTTTGGAG 
AGTAAGAATG AGTTGAACAC GCTGACAGAT CAAATTGAAT CTTTGAATAG TGAGGTGGAG 
AATAAGAATG AGGAAATTCG AAATTTGATG GCTACACTTC AAGAAAAAGA AGTTCACATT 
CAAAACGTCC GAACATCAAG TCATCAGTTG ACTGCCACAT ACGAAGAGGC TAACGGGGAA 
ATCGATATTT TGAAGGCTGA ACTTACCCGG CTCCACGAGC AAGTCAATGA GAGGACTCGA 
CAGATCTCCG AAGCTAATGA GAAGTATGAT GATGCAGCTC GGAAGAATGA CGCATTGCTC 
GAAGATGTCG CCACGTGGCA AGAGAAGTAT GAGCAGTTGA AGATGGAGCT GGAAGAAATG 
AACAGAAGAG GACAGGAGAA GGAACGAGAA GAAGCAGATC TTCGAGCATT ACTTGATGAT 
TTACGTGGAA ACTTTGATAA GTTGACTAAT GAGCTGAAGC AGAAGGGTGT TACTGTTGAT 
AGCTTGAATG AAGAAATTTC ATCGTTGAAA GAACAATTAA ACAAGTCAGA AAAGGAGAGA 
AAAGAAGAAC TGTTAAGGAT GGAAGAGCTT GAACAGAAAA ATGAAGCGGA AATGAAAGAA 
GAGTATGAAG TAAAGTTACA ATTGGCTGAG AAGGATCGTC AAGGAGTTGA GAATTTTGGA 
AAAGAATGTG AAGCGAGAAT GAATGAGCTC ACGAAGATCC ACGAGATGCT AATGGAAGAG 
CACGATCAGT TGAAAGTGGA TCATCTGCAC ACGGAAGAAG AAGTGGAGAG GTTGAAAGAG 
AAAATGAGAA AGGAATTGGA GAAGTTGAAT GAGCAGAATG ATGGTGATCG GGCGGAATGG 
AGTAATGAAA GGAATCGGCT GGAATCATCC AAAAACGAAG CAGTTACGGA ACTTCAAGAA 
CGAGTGCAGA AGCTTGAGGA TGTGGTGAAG GAGAAAGAAG ATAAGGAAAT TGCTCTTCGT 
AGGGATTTAG AAGACAGCCA TGAGAAATCA AGAGATCTTG ATGACAAGCT TCGAAAAATG 
GAGTTGACAG ATGAGGAGAA AGAGGAAGAT CGGAAGAAGG AACAGAAGAC ATTGAATGAA 
GAGAGGATGA AACTCATGGA ACAGAAAGAA GAAGCAATGC TTGTTGCTAC AAAACATGCA 
ACAACGATTG ATCAACAGAC TCGAAGAATT TCAGTGTTGG AGGGTGACGT GGAAAAGCTG 
ACGGCTGGAA TTGCGGAGAG AGAAAGTTCG ATTAATGCTT TGGAATCGAA TACAATGGAG 
CTGATATCCA AGCTGGAAAC TACTGAAGCA GAACTTGAAA AGCTGAAAGA CGAGCTTGCA 
GTCATGCTGA AACAAAACTC GGAACTCAAA AATGGAAAAG AGGGGTTGAG TGAGAAGTGG 
AATGAGGAGA GGAAGAAGAT CCAAGACTTG GCAGATCAAT TGAGAGAGGC TAACAAAGTT 
GTTCATAATA TGAGAATGAA GAATGTTAAT CTAGAAGAAA AGAAGAATGA ATTGGATCAG 
AATGTGACTG ATTTGACCAA TAAAGTTCGA CAATTAGAGA TTCAGTTGAT GGATAAAGCT 
GCGAAGAACG AGGTTTCAGG AGATTTGCTC AGAAAGATGG AGCATGATGC TCAGAGTATG 
CTGAAGCAAG CACAGAATGA GCAGTTTAGA CTAACTGATC TTGAAAAAGT TCGAAAAGCT 
CTTCAAGATG AGAATCAACG GCTTGTAAAT GATCTGGCAA CAGTGAAAGC TGCATTTGAA 
GTCAAGAGAG AAACGAGCAA ATCTGCAATC AGTGACATTT TGGATAAGTA CCGGAGTGCT 
GAGGAGAAGG CCAATAAGGG TGAATTGGAT AATCAGAGGC TTAGAAGTGA TCTGGCTACT 
GTGACATTAA AACTGGAACG TCAAGAACTG AAAGCAAAAG ATTCTGATAA TCGACTGAGA 
GATAGCCAAA AACGATTCGA AGAAGTTCAA TCAAAACTTG CAAATCTTCA GAAATCAGCA 
GTAGAATCCT TACAAAATCC TATGTCTTCG AATTCCCGCC AAAATCGGTC AATCTATGTT 
GACATTCCAC GTGCAGCTTC TTCGATCGGC CTCAATGAGA ATTCTGATGA AGTCCCGCTT 
CGCAGTTCCC CCTCAGTCCG TTTTGCAGAC AGCTCACAGA ATATGCAAAG AGCAGTTGAC 
TCTATGGATG TGAGTTCTTC AGTTGGAGTT ACACTTCGGT TTTTGAAAGA AAGAATCGAA 
CAGTTGGAAG CAGACAATGC AGATTTATCA GATGCTCTGG AAAAGGCAAA AGATGAGCTG 
AGGCAGAGAA ATGAGAAGCT GGCGGATAGG CAAATGGTTA TTGAGAGAGT TGAGAGACAA 
TTGGTTCATA TTACTGAAGA ACGCAATACA ATTGAAAATC GAATGACAAG TCAGCGTCAA 
ATGTATCTCA CAAATGAGGA ATCATCACGA TCAAGAGAAC ATGAAATCAG AAGTATGAAA 
GCCAGAATTT CAACTTTAGA GCTTCATCTT CGAGAAAAAG AATCTAAACT TGCACATCTA 
CGAAAAGAAA TTGAAGTACT TCATGGTCAG CTTCATGATG CTCTCGAATC AAAGGAAAAG 
GCTACTGGAT TGGTTGGAGT TCAAGATTCA AAGCATCGAG ACTTAGAAGA ACAACTCGAT 
CGAGCAAATC GTGAACGAGA ATTGGCAATT GGGAAGCAGA GAAGAACTCT TGCAGAGAAC 
GAGAATCTGT TCCGGAAGCT TGAACAATTG GAAAAGGAAA GAGAGCAGCT TATGCGAGAG 
ATCACAGATG AACGGAGGCT CAACGAGCGG AATCGCACAA GTCTTGAAGA ACTTCGAGTC 
TCTGAAAGAA CATGGAAATC AGCCATGACA ACTGCCAAAA AACCAGCTGA GGAGCAGGAA 
CGGGCGGTGC AGGAGCAACG GAGATGGGAA GAGTCAAATC ATGAAATGAC GAATAGAAAT 
ACTGCATTGA CAAAAGAATG TGATAGATTA CGAGTTGAAA TGAGAGATCA ACTCAACAGA 
ATGAACGGGA TCAATTTGAG ATCAGTAGAT TTCGAGAGAA AGAATGAAGA GCTTTCAAGT 
AAACTGATTG TTATGCAAAA TACAGTAACT GCGATGAAAA AGTTCGAGGA AGAATGGAAG 
AGGTTGGAGG CGGAGATGAG AGCAGAACTT AAGATTTTGA GAAAAGAAAA GCTCATGCAA 
ACTGCTGAAA TTGAGGATTT AAAGAGGAAA AGCTTCAGAA GTGACACTGA AAAGAAGGAG 
ATCGAGGGTA TTCGAGTAAG GTTGGAAAGG GAAATCAGTG CACTTAAGAG ACATGTTGAT 
GCGCTAGAAG AAGAGAAGGG AAAAACTGAA AAGGCCGTTC GAGAAACCAT GAACGAACGT 
CGTGCCATCG ACAAATCTCT CGCGTCAATG GAACGAGAAA ATCAACAGTT GTATAGGAAT 
TGTGCTCAAC TTCAAGCTCA AATTCAGAAT CTGGAGCGCG ACGCCGGAAA TCGGTCGGTA 
ACCAAGTTGG CTAAGGAGCA TTCATTGCTC GAAGCTCGAA TTGCAGCTTT AATTGAAGAA 
AAACGTCAGC TCCAATCAAT GCTTGACCAA AAAGACGCCA ACTACTCTCA CAAGCGAAAA 
CTCTTAGAAT CCCAAATCCA GTTGCTCCGC GAGCAACTCG AAGCCGAACG CCGAAAGCGT 
ACAAAGGGAG TTGTGGCGAC TGGTCCCACC GTTAGCCGAC GGGGCGTTCA GCACACATCT 
GCCTTTAGGC ACACCATCGA GCGTCATCGG TCTCTGTCCC AGTCTAGTGA ACGGACAATT 
CTTCAGGAAC GATACCTTGA ATATGTATAT ACAGGAGATC GTACTCCAGC AATACAAATG 
ATCAACACAC CGCCAATCTC TCCATTATCA CATTCTGGCT CTTTCAATTC GGACGGAAGG 
GAAGCCAGAA TCCGGAGAGA GTCTGACAAC GGCCATCTTC AGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_494819.3
CDS	1..5868
Translation	MKSAASSEDVVRVTEDLSECRNRLDAGIEENRRNRQVIQDINDQLQRFRQRANAESIESFNLTPSPDVTLSSLAHPGLTHLHNQTNISMPSLTIDIPLNSNAMISSSRTPNYAINGLRNRHKSLVGHRYRSTSPIGDYGRHRSSPRVLAHYNLDGADIGVGEENLDELFAKLKEELFKNNTLEEVNEMLREENDAALAANEHLRVDATNLSRQLQQLQQQQHTESMRFRSENTRYRNQTETQHRKLISLWKEFTAVKRQLHELRTTTANDLDRQLTEFTRCATLMRKAIRHAEQKNLDQKEQMKREKDDVLDETLRQLNSVTENYMKSEEKANERQRDLKRKEDECRKLREQNDELSDILEQLSKMAHEMAGGRGRNETPMDVARKMRKLLTTKNGEIDESREAAKQAEKERDRAKKDLEKEEKRRKDDREAERKRSSVYSQREHDLKKLDDELRKASEKIRNLEEQRESQEKLTISVQNSLNEAHRQHKQFIEELMIRHREELKEREDSHEEALRSKDTEERSRFEKERSEREKIRRESDELRETQRSLKGDVAAMKTDLDDKTLRLDMLETERDELKKKLETEREQADQRDLEIAECRAKLDEMAEKEAELRKELAEFQAIITAMEGEGKLNQEQFLESKNELNTLTDQIESLNSEVENKNEEIRNLMATLQEKEVHIQNVRTSSHQLTATYEEANGEIDILKAELTRLHEQVNERTRQISEANEKYDDAARKNDALLEDVATWQEKYEQLKMELEEMNRRGQEKEREEADLRALLDDLRGNFDKLTNELKQKGVTVDSLNEEISSLKEQLNKSEKERKEELLRMEELEQKNEAEMKEEYEVKLQLAEKDRQGVENFGKECEARMNELTKIHEMLMEEHDQLKVDHLHTEEEVERLKEKMRKELEKLNEQNDGDRAEWSNERNRLESSKNEAVTELQERVQKLEDVVKEKEDKEIALRRDLEDSHEKSRDLDDKLRKMELTDEEKEEDRKKEQKTLNEERMKLMEQKEEAMLVATKHATTIDQQTRRISVLEGDVEKLTAGIAERESSINALESNTMELISKLETTEAELEKLKDELAVMLKQNSELKNGKEGLSEKWNEERKKIQDLADQLREANKVVHNMRMKNVNLEEKKNELDQNVTDLTNKVRQLEIQLMDKAAKNEVSGDLLRKMEHDAQSMLKQAQNEQFRLTDLEKVRKALQDENQRLVNDLATVKAAFEVKRETSKSAISDILDKYRSAEEKANKGELDNQRLRSDLATVTLKLERQELKAKDSDNRLRDSQKRFEEVQSKLANLQKSAVESLQNPMSSNSRQNRSIYVDIPRAASSIGLNENSDEVPLRSSPSVRFADSSQNMQRAVDSMDVSSSVGVTLRFLKERIEQLEADNADLSDALEKAKDELRQRNEKLADRQMVIERVERQLVHITEERNTIENRMTSQRQMYLTNEESSRSREHEIRSMKARISTLELHLREKESKLAHLRKEIEVLHGQLHDALESKEKATGLVGVQDSKHRDLEEQLDRANRERELAIGKQRRTLAENENLFRKLEQLEKEREQLMREITDERRLNERNRTSLEELRVSERTWKSAMTTAKKPAEEQERAVQEQRRWEESNHEMTNRNTALTKECDRLRVEMRDQLNRMNGINLRSVDFERKNEELSSKLIVMQNTVTAMKKFEEEWKRLEAEMRAELKILRKEKLMQTAEIEDLKRKSFRSDTEKKEIEGIRVRLEREISALKRHVDALEEEKGKTEKAVRETMNERRAIDKSLASMERENQQLYRNCAQLQAQIQNLERDAGNRSVTKLAKEHSLLEARIAALIEEKRQLQSMLDQKDANYSHKRKLLESQIQLLREQLEAERRKRTKGVVATGPTVSRRGVQHTSAFRHTIERHRSLSQSSERTILQERYLEYVYTGDRTPAIQMINTPPISPLSHSGSFNSDGREARIRRESDNGHLQH

Target ORF information:

RefSeq Version	NM_062418.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Rootletin (che-10), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_062418.6

ORF Insert Sequence:

ATGAAATCGG CGGCAAGTTC GGAGGATGTC GTTCGTGTCA CTGAAGATCT ATCCGAATGC 
CGAAATCGGT TGGATGCTGG GATTGAGGAG AATCGGAGGA ATCGGCAGGT TATCCAGGAT 
ATTAATGATC AGCTCCAACG CTTCCGCCAA AGAGCAAACG CAGAATCAAT CGAGTCTTTC 
AATCTGACCC CCAGCCCCGA TGTGACATTA TCATCACTTG CTCATCCAGG ACTCACACAT 
CTTCACAATC AGACAAACAT TTCCATGCCT TCTCTAACCA TTGACATTCC GTTGAACTCC 
AATGCCATGA TTAGTTCATC TAGAACCCCA AACTATGCGA TTAATGGGTT AAGAAATCGA 
CACAAAAGTC TAGTAGGGCA TAGGTATCGG TCGACGAGCC CGATCGGAGA TTATGGAAGA 
CATCGAAGCA GTCCAAGAGT TTTGGCTCAT TATAATTTGG ATGGTGCAGA TATTGGAGTT 
GGAGAGGAGA ATTTGGACGA ACTTTTTGCA AAGTTGAAAG AGGAATTGTT CAAAAACAAC 
ACACTGGAAG AGGTGAATGA GATGCTCCGA GAAGAAAATG ATGCAGCACT AGCTGCAAAT 
GAGCATTTAA GAGTTGATGC TACTAATTTG AGCAGACAAC TACAGCAATT ACAACAGCAG 
CAGCATACAG AGTCGATGAG ATTTCGGTCG GAGAATACGC GCTATCGAAA TCAAACGGAG 
ACCCAACACA GAAAGCTGAT CAGTCTTTGG AAGGAGTTCA CCGCTGTCAA GCGTCAGCTC 
CACGAGTTGC GCACAACCAC CGCCAACGAT TTGGACAGAC AATTGACCGA ATTCACGCGG 
TGTGCAACAC TTATGCGAAA AGCCATTCGC CACGCCGAGC AAAAGAATCT CGACCAGAAG 
GAACAAATGA AACGAGAAAA GGACGATGTA CTCGATGAGA CACTTCGTCA GCTCAACTCT 
GTCACTGAGA ATTATATGAA ATCAGAAGAA AAAGCAAACG AACGGCAGCG AGATTTGAAG 
AGAAAAGAGG ACGAATGCAG GAAGCTCCGC GAGCAAAACG ACGAGCTCTC GGATATTCTC 
GAACAACTTT CCAAAATGGC TCACGAAATG GCTGGAGGCA GAGGAAGAAA CGAAACACCA 
ATGGATGTGG CACGTAAAAT GCGGAAGCTG CTGACCACCA AGAACGGGGA AATCGATGAA 
TCAAGGGAAG CAGCGAAGCA GGCAGAAAAG GAAAGAGATC GTGCAAAGAA GGATTTGGAA 
AAAGAAGAGA AACGAAGAAA AGATGACAGA GAAGCAGAGA GAAAACGATC AAGTGTCTAT 
TCACAGAGAG AACATGACCT GAAAAAGTTG GATGATGAGT TGAGGAAGGC CAGTGAAAAG 
ATCCGGAATC TCGAGGAGCA ACGGGAGAGC CAGGAAAAAC TCACCATATC AGTTCAGAAC 
TCATTGAATG AAGCACACAG GCAACACAAA CAGTTTATTG AGGAATTGAT GATTCGGCAT 
CGAGAAGAAC TGAAAGAGCG GGAGGATTCT CACGAGGAAG CGTTACGTTC CAAAGATACC 
GAGGAAAGAT CTCGATTTGA AAAAGAACGT TCTGAACGGG AAAAGATTAG ACGAGAAAGT 
GATGAGCTCA GAGAGACGCA GAGAAGTTTA AAGGGTGACG TGGCGGCGAT GAAGACAGAT 
TTGGATGATA AGACTTTAAG GCTTGACATG CTCGAAACAG AACGGGATGA GCTCAAAAAG 
AAACTGGAAA CCGAAAGAGA GCAGGCTGAT CAGAGAGACT TGGAGATAGC AGAGTGTCGA 
GCAAAGCTTG ATGAGATGGC TGAGAAGGAA GCGGAGCTCC GGAAAGAGCT CGCAGAGTTC 
CAGGCAATTA TCACTGCGAT GGAAGGAGAA GGAAAGCTGA ATCAAGAACA GTTTTTGGAG 
AGTAAGAATG AGTTGAACAC GCTGACAGAT CAAATTGAAT CTTTGAATAG TGAGGTGGAG 
AATAAGAATG AGGAAATTCG AAATTTGATG GCTACACTTC AAGAAAAAGA AGTTCACATT 
CAAAACGTCC GAACATCAAG TCATCAGTTG ACTGCCACAT ACGAAGAGGC TAACGGGGAA 
ATCGATATTT TGAAGGCTGA ACTTACCCGG CTCCACGAGC AAGTCAATGA GAGGACTCGA 
CAGATCTCCG AAGCTAATGA GAAGTATGAT GATGCAGCTC GGAAGAATGA CGCATTGCTC 
GAAGATGTCG CCACGTGGCA AGAGAAGTAT GAGCAGTTGA AGATGGAGCT GGAAGAAATG 
AACAGAAGAG GACAGGAGAA GGAACGAGAA GAAGCAGATC TTCGAGCATT ACTTGATGAT 
TTACGTGGAA ACTTTGATAA GTTGACTAAT GAGCTGAAGC AGAAGGGTGT TACTGTTGAT 
AGCTTGAATG AAGAAATTTC ATCGTTGAAA GAACAATTAA ACAAGTCAGA AAAGGAGAGA 
AAAGAAGAAC TGTTAAGGAT GGAAGAGCTT GAACAGAAAA ATGAAGCGGA AATGAAAGAA 
GAGTATGAAG TAAAGTTACA ATTGGCTGAG AAGGATCGTC AAGGAGTTGA GAATTTTGGA 
AAAGAATGTG AAGCGAGAAT GAATGAGCTC ACGAAGATCC ACGAGATGCT AATGGAAGAG 
CACGATCAGT TGAAAGTGGA TCATCTGCAC ACGGAAGAAG AAGTGGAGAG GTTGAAAGAG 
AAAATGAGAA AGGAATTGGA GAAGTTGAAT GAGCAGAATG ATGGTGATCG GGCGGAATGG 
AGTAATGAAA GGAATCGGCT GGAATCATCC AAAAACGAAG CAGTTACGGA ACTTCAAGAA 
CGAGTGCAGA AGCTTGAGGA TGTGGTGAAG GAGAAAGAAG ATAAGGAAAT TGCTCTTCGT 
AGGGATTTAG AAGACAGCCA TGAGAAATCA AGAGATCTTG ATGACAAGCT TCGAAAAATG 
GAGTTGACAG ATGAGGAGAA AGAGGAAGAT CGGAAGAAGG AACAGAAGAC ATTGAATGAA 
GAGAGGATGA AACTCATGGA ACAGAAAGAA GAAGCAATGC TTGTTGCTAC AAAACATGCA 
ACAACGATTG ATCAACAGAC TCGAAGAATT TCAGTGTTGG AGGGTGACGT GGAAAAGCTG 
ACGGCTGGAA TTGCGGAGAG AGAAAGTTCG ATTAATGCTT TGGAATCGAA TACAATGGAG 
CTGATATCCA AGCTGGAAAC TACTGAAGCA GAACTTGAAA AGCTGAAAGA CGAGCTTGCA 
GTCATGCTGA AACAAAACTC GGAACTCAAA AATGGAAAAG AGGGGTTGAG TGAGAAGTGG 
AATGAGGAGA GGAAGAAGAT CCAAGACTTG GCAGATCAAT TGAGAGAGGC TAACAAAGTT 
GTTCATAATA TGAGAATGAA GAATGTTAAT CTAGAAGAAA AGAAGAATGA ATTGGATCAG 
AATGTGACTG ATTTGACCAA TAAAGTTCGA CAATTAGAGA TTCAGTTGAT GGATAAAGCT 
GCGAAGAACG AGGTTTCAGG AGATTTGCTC AGAAAGATGG AGCATGATGC TCAGAGTATG 
CTGAAGCAAG CACAGAATGA GCAGTTTAGA CTAACTGATC TTGAAAAAGT TCGAAAAGCT 
CTTCAAGATG AGAATCAACG GCTTGTAAAT GATCTGGCAA CAGTGAAAGC TGCATTTGAA 
GTCAAGAGAG AAACGAGCAA ATCTGCAATC AGTGACATTT TGGATAAGTA CCGGAGTGCT 
GAGGAGAAGG CCAATAAGGG TGAATTGGAT AATCAGAGGC TTAGAAGTGA TCTGGCTACT 
GTGACATTAA AACTGGAACG TCAAGAACTG AAAGCAAAAG ATTCTGATAA TCGACTGAGA 
GATAGCCAAA AACGATTCGA AGAAGTTCAA TCAAAACTTG CAAATCTTCA GAAATCAGCA 
GTAGAATCCT TACAAAATCC TATGTCTTCG AATTCCCGCC AAAATCGGTC AATCTATGTT 
GACATTCCAC GTGCAGCTTC TTCGATCGGC CTCAATGAGA ATTCTGATGA AGTCCCGCTT 
CGCAGTTCCC CCTCAGTCCG TTTTGCAGAC AGCTCACAGA ATATGCAAAG AGCAGTTGAC 
TCTATGGATG TGAGTTCTTC AGTTGGAGTT ACACTTCGGT TTTTGAAAGA AAGAATCGAA 
CAGTTGGAAG CAGACAATGC AGATTTATCA GATGCTCTGG AAAAGGCAAA AGATGAGCTG 
AGGCAGAGAA ATGAGAAGCT GGCGGATAGG CAAATGGTTA TTGAGAGAGT TGAGAGACAA 
TTGGTTCATA TTACTGAAGA ACGCAATACA ATTGAAAATC GAATGACAAG TCAGCGTCAA 
ATGTATCTCA CAAATGAGGA ATCATCACGA TCAAGAGAAC ATGAAATCAG AAGTATGAAA 
GCCAGAATTT CAACTTTAGA GCTTCATCTT CGAGAAAAAG AATCTAAACT TGCACATCTA 
CGAAAAGAAA TTGAAGTACT TCATGGTCAG CTTCATGATG CTCTCGAATC AAAGGAAAAG 
GCTACTGGAT TGGTTGGAGT TCAAGATTCA AAGCATCGAG ACTTAGAAGA ACAACTCGAT 
CGAGCAAATC GTGAACGAGA ATTGGCAATT GGGAAGCAGA GAAGAACTCT TGCAGAGAAC 
GAGAATCTGT TCCGGAAGCT TGAACAATTG GAAAAGGAAA GAGAGCAGCT TATGCGAGAG 
ATCACAGATG AACGGAGGCT CAACGAGCGG AATCGCACAA GTCTTGAAGA ACTTCGAGTC 
TCTGAAAGAA CATGGAAATC AGCCATGACA ACTGCCAAAA AACCAGCTGA GGAGCAGGAA 
CGGGCGGTGC AGGAGCAACG GAGATGGGAA GAGTCAAATC ATGAAATGAC GAATAGAAAT 
ACTGCATTGA CAAAAGAATG TGATAGATTA CGAGTTGAAA TGAGAGATCA ACTCAACAGA 
ATGAACGGGA TCAATTTGAG ATCAGTAGAT TTCGAGAGAA AGAATGAAGA GCTTTCAAGT 
AAACTGATTG TTATGCAAAA TACAGTAACT GCGATGAAAA AGTTCGAGGA AGAATGGAAG 
AGGTTGGAGG CGGAGATGAG AGCAGAACTT AAGATTTTGA GAAAAGAAAA GCTCATGCAA 
ACTGCTGAAA TTGAGGATTT AAAGAGGAAA AGCTTCAGAA GTGACACTGA AAAGAAGGAG 
ATCGAGGGTA TTCGAGTAAG GTTGGAAAGG GAAATCAGTG CACTTAAGAG ACATGTTGAT 
GCGCTAGAAG AAGAGAAGGG AAAAACTGAA AAGGCCGTTC GAGAAACCAT GAACGAACGT 
CGTGCCATCG ACAAATCTCT CGCGTCAATG GAACGAGAAA ATCAACAGTT GTATAGGAAT 
TGTGCTCAAC TTCAAGCTCA AATTCAGAAT CTGGAGCGCG ACGCCGGAAA TCGGTCGGTA 
ACCAAGTTGG CTAAGGAGCA TTCATTGCTC GAAGCTCGAA TTGCAGCTTT AATTGAAGAA 
AAACGTCAGC TCCAATCAAT GCTTGACCAA AAAGACGCCA ACTACTCTCA CAAGCGAAAA 
CTCTTAGAAT CCCAAATCCA GTTGCTCCGC GAGCAACTCG AAGCCGAACG CCGAAAGCGT 
ACAAAGGGAG TTGTGGCGAC TGGTCCCACC GTTAGCCGAC GGGGCGTTCA GCACACATCT 
GCCTTTAGGC ACACCATCGA GCGTCATCGG TCTCTGTCCC AGTCTAGTGA ACGGACAATT 
CTTCAGGAAC GATACCTTGA ATATGTATAT ACAGGAGATC GTACTCCAGC AATACAAATG 
ATCAACACAC CGCCAATCTC TCCATTATCA CATTCTGGCT CTTTCAATTC GGACGGAAGG 
GAAGCCAGAA TCCGGAGAGA GTCTGACAAC GGCCATCTTC AGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

che-10 ( NM_062419.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_494820.3 Caenorhabditis elegans Rootletin (che-10), partial mRNA.

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CloneID	OCa339204
Clone ID Related Accession (Same CDS sequence)	NM_062419.5 , NM_062419.6
Accession Version	NM_062419.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Rootletin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_062419.4. COMPLETENESS: incomplete on both ends.

ATGAAATCGG CGGCAAGTTC GGAGGATGTC GTTCGTGTCA CTGAAGATCT ATCCGAATGC 
CGAAATCGGT TGGATGCTGG GATTGAGGAG AATCGGAGGA ATCGGCAGGT TATCCAGGAT 
ATTAATGATC AGCTCCAACG CTTCCGCCAA AGAGCAAACG CAGAATCAAT CGAGTCTTTC 
AATCTGACCC CCAGCCCCGA TGTGACATTA TCATCACTTG CTCATCCAGG ACTCACACAT 
CTTCACAATC AGACAAACAT TTCCATGCCT TCTCTAACCA TTGACATTCC GTTGAACTCC 
AATGCCATGA TTAGTTCATC TAGAACCCCA AACTATGCGA TTAATGGGTT AAGAAATCGA 
CACAAAAGTC TAGTAGGGCA TAGGTATCGG TCGACGAGCC CGATCGGAGA TTATGGAAGA 
CATCGAAGCA GTCCAAGAGT TTTGGCTCAT TATAATTTGG ATGGTGCAGA TATTGGAGTT 
GGAGAGGAGA ATTTGGACGA ACTTTTTGCA AAGTTGAAAG AGGAATTGTT CAAAAACAAC 
ACACTGGAAG AGGTGAATGA GATGCTCCGA GAAGAAAATG ATGCAGCACT AGCTGCAAAT 
GAGCATTTAA GAGTTGATGC TACTAATTTG AGCAGACAAC TACAGCAATT ACAACAGCAG 
CAGCATACAG AGTCGATGAG ATTTCGGTCG GAGAATACGC GCTATCGAAA TCAAACGGAG 
ACCCAACACA GAAAGCTGAT CAGTCTTTGG AAGGAGTTCA CCGCTGTCAA GCGTCAGCTC 
CACGAGTTGC GCACAACCAC CGCCAACGAT TTGGACAGAC AATTGACCGA ATTCACGCGG 
TGTGCAACAC TTATGCGAAA AGCCATTCGC CACGCCGAGC AAAAGAATCT CGACCAGAAG 
GAACAAATGA AACGAGAAAA GGACGATGTA CTCGATGAGA CACTTCGTCA GCTCAACTCT 
GTCACTGAGA ATTATATGAA ATCAGAAGAA AAAGCAAACG AACGGCAGCG AGATTTGAAG 
AGAAAAGAGG ACGAATGCAG GAAGCTCCGC GAGCAAAACG ACGAGCTCTC GGATATTCTC 
GAACAACTTT CCAAAATGGC TCACGAAATG GCTGGAGGCA GAGGAAGAAA CGAAACACCA 
ATGGATGTGG CACGTAAAAT GCGGAAGCTG CTGACCACCA AGAACGGGGA AATCGATGAA 
TCAAGGGAAG CAGCGAAGCA GGCAGAAAAG GAAAGAGATC GTGCAAAGAA GGATTTGGAA 
AAAGAAGAGA AACGAAGAAA AGATGACAGA GAAGCAGAGA GAAAACGATC AAGTGTCTAT 
TCACAGAGAG AACATGACCT GAAAAAGTTG GATGATGAGT TGAGGAAGGC CAGTGAAAAG 
ATCCGGAATC TCGAGGAGCA ACGGGAGAGC CAGGAAAAAC TCACCATATC AGTTCAGAAC 
TCATTGAATG AAGCACACAG GCAACACAAA CAGTTTATTG AGGAATTGAT GATTCGGCAT 
CGAGAAGAAC TGAAAGAGCG GGAGGATTCT CACGAGGAAG CGTTACGTTC CAAAGATACC 
GAGGAAAGAT CTCGATTTGA AAAAGAACGT TCTGAACGGG AAAAGATTAG ACGAGAAAGT 
GATGAGCTCA GAGAGACGCA GAGAAGTTTA AAGGGTGACG TGGCGGCGAT GAAGACAGAT 
TTGGATGATA AGACTTTAAG GCTTGACATG CTCGAAACAG AACGGGATGA GCTCAAAAAG 
AAACTGGAAA CCGAAAGAGA GCAGGCTGAT CAGAGAGACT TGGAGATAGC AGAGTGTCGA 
GCAAAGCTTG ATGAGATGGC TGAGAAGGAA GCGGAGCTCC GGAAAGAGCT CGCAGAGTTC 
CAGGCAATTA TCACTGCGAT GGAAGGAGAA GGAAAGCTGA ATCAAGAACA GTTTTTGGAG 
AGTAAGAATG AGTTGAACAC GCTGACAGAT CAAATTGAAT CTTTGAATAG TGAGGTGGAG 
AATAAGAATG AGGAAATTCG AAATTTGATG GCTACACTTC AAGAAAAAGA AGTTCACATT 
CAAAACGTCC GAACATCAAG TCATCAGTTG ACTGCCACAT ACGAAGAGGC TAACGGGGAA 
ATCGATATTT TGAAGGCTGA ACTTACCCGG CTCCACGAGC AAGTCAATGA GAGGACTCGA 
CAGATCTCCG AAGCTAATGA GAAGTATGAT GATGCAGCTC GGAAGAATGA CGCATTGCTC 
GAAGATGTCG CCACGTGGCA AGAGAAGTAT GAGCAGTTGA AGATGGAGCT GGAAGAAATG 
AACAGAAGAG GACAGGAGAA GGAACGAGAA GAAGCAGATC TTCGAGCATT ACTTGATGAT 
TTACGTGGAA ACTTTGATAA GTTGACTAAT GAGCTGAAGC AGAAGGGTGT TACTGTTGAT 
AGCTTGAATG AAGAAATTTC ATCGTTGAAA GAACAATTAA ACAAGTCAGA AAAGGAGAGA 
AAAGAAGAAC TGTTAAGGAT GGAAGAGCTT GAACAGAAAA ATGAAGCGGA AATGAAAGAA 
GAGTATGAAG TAAAGTTACA ATTGGCTGAG AAGGATCGTC AAGGAGTTGA GAATTTTGGA 
AAAGAATGTG AAGCGAGAAT GAATGAGCTC ACGAAGATCC ACGAGATGCT AATGGAAGAG 
CACGATCAGT TGAAAGTGGA TCATCTGCAC ACGGAAGAAG AAGTGGAGAG GTTGAAAGAG 
AAAATGAGAA AGGAATTGGA GAAGTTGAAT GAGCAGAATG ATGGTGATCG GGCGGAATGG 
AGTAATGAAA GGAATCGGCT GGAATCATCC AAAAACGAAG CAGTTACGGA ACTTCAAGAA 
CGAGTGCAGA AGCTTGAGGA TGTGGTGAAG GAGAAAGAAG ATAAGGAAAT TGCTCTTCGT 
AGGGATTTAG AAGACAGCCA TGAGAAATCA AGAGATCTTG ATGACAAGCT TCGAAAAATG 
GAGTTGACAG ATGAGGAGAA AGAGGAAGAT CGGAAGAAGG AACAGAAGAC ATTGAATGAA 
GAGAGGATGA AACTCATGGA ACAGAAAGAA GAAGCAATGC TTGTTGCTAC AAAACATGCA 
ACAACGATTG ATCAACAGAC TCGAAGAATT TCAGTGTTGG AGGGTGACGT GGAAAAGCTG 
ACGGCTGGAA TTGCGGAGAG AGAAAGTTCG ATTAATGCTT TGGAATCGAA TACAATGGAG 
CTGATATCCA AGCTGGAAAC TACTGAAGCA GAACTTGAAA AGCTGAAAGA CGAGCTTGCA 
GTCATGCTGA AACAAAACTC GGAACTCAAA AATGGAAAAG AGGGGTTGAG TGAGAAGTGG 
AATGAGGAGA GGAAGAAGAT CCAAGACTTG GCAGATCAAT TGAGAGAGGC TAACAAAGTT 
GTTCATAATA TGAGAATGAA GAATGTTAAT CTAGAAGAAA AGAAGAATGA ATTGGATCAG 
AATGTGACTG ATTTGACCAA TAAAGTTCGA CAATTAGAGA TTCAGTTGAT GGATAAAGCT 
GCGAAGAACG AGGTTTCAGG AGATTTGCTC AGAAAGATGG AGCATGATGC TCAGAGTATG 
CTGAAGCAAG CACAGAATGA GCAGTTTAGA CTAACTGATC TTGAAAAAGT TCGAAAAGCT 
CTTCAAGATG AGAATCAACG GCTTGTAAAT GATCTGGCAA CAGTGAAAGC TGCATTTGAA 
GTCAAGAGAG AAACGAGCAA ATCTGCAATC AGTGACATTT TGGATAAGTA CCGGAGTGCT 
GAGGAGAAGG CCAATAAGGG TGAATTGGAT AATCAGAGGC TTAGAAGTGA TCTGGCTACT 
GTGACATTAA AACTGGAACG TCAAGAACTG AAAGCAAAAG ATTCTGATAA TCGACTGAGA 
GATAGCCAAA AACGATTCGA AGAAGTTCAA TCAAAACTTG CAAATCTTCA GAAATCAGCA 
GTAGAATCCT TACAAAATCC TATGTCTTCG AATTCCCGCC AAAATCGGTC AATCTATGTT 
GACATTCCAC GTGCAGCTTC TTCGATCGGC CTCAATGAGA ATTCTGATGA AGTCCCGCTT 
CGCAGTTCCC CCTCAGTCCG TTTTGCAGAC AGCTCACAGA ATATGCAAAG AGCAGTTGAC 
TCTATGGATG TGAGTTCTTC AGTTGGAGTT ACACTTCGGT TTTTGAAAGA AAGAATCGAA 
CAGTTGGAAG CAGACAATGC AGATTTATCA GATGCTCTGG AAAAGGCAAA AGATGAGCTG 
AGGCAGAGAA ATGAGAAGCT GGCGGATAGG CAAATGGTTA TTGAGAGAGT TGAGAGACAA 
TTGGTTCATA TTACTGAAGA ACGCAATACA ATTGAAAATC GAATGACAAG TCAGCGTCAA 
ATGTATCTCA CAAATGAGGA ATCATCACGA TCAAGAGAAC ATGAAATCAG AAGTATGAAA 
GCCAGAATTT CAACTTTAGA GCTTCATCTT CGAGAAAAAG AATCTAAACT TGCACATCTA 
CGAAAAGAAA TTGAAGTACT TCATGGTCAG CTTCATGATG CTCTCGAATC AAAGGAAAAG 
GCTACTGGAT TGGTTGGAGT TCAAGATTCA AAGCATCGAG ACTTAGAAGA ACAACTCGAT 
CGAGCAAATC GTGAACGAGA ATTGGCAATT GGGAAGCAGA GAAGAACTCT TGCAGAGAAC 
GAGAATCTGT TCCGGAAGCT TGAACAATTG GAAAAGGAAA GAGAGCAGCT TATGCGAGAG 
ATCACAGATG AACGGAGGCT CAACGAGCGG AATCGCACAA GTCTTGAAGA ACTTCGAGTC 
TCTGAAAGAA CATGGAAATC AGCCATGACA ACTGCCAAAA AACCAGCTGA GGAGCAGGAA 
CGGGCGGTGC AGGAGCAACG GAGATGGGAA GAGTCAAATC ATGAAATGAC GAATAGAAAT 
ACTGCATTGA CAAAAGAATG TGATAGATTA CGAGTTGAAA TGAGAGATCA ACTCAACAGA 
ATGAACGGGA TCAATTTGAG ATCAGTAGAT TTCGAGAGAA AGAATGAAGA GCTTTCAAGT 
AAACTGATTG TTATGCAAAA TACAGTAACT GCGATGAAAA AGTTCGAGGA AGAATGGAAG 
AGGTTGGAGG CGGAGATGAG AGCAGAACTT AAGATTTTGA GAAAAGAAAA GCTCATGCAA 
ACTGCTGAAA TTGAGGATTT AAAGAGGAAA AGCTTCAGAA GTGACACTGA AAAGAAGGAG 
ATCGAGGGTA TTCGAGTAAG GTTGGAAAGG GAAATCAGTG CACTTAAGAG ACATGTTGAT 
GCGCTAGAAG AAGAGAAGGG AAAAACTGAA AAGGCCGTTC GAGAAACCAT GAACGAACGT 
CGTGCCATCG ACAAATCTCT CGCGTCAATG GAACGAGAAA ATCAACAGTT GTATAGGAAT 
TGTGCTCAAC TTCAAGCTCA AATTCAGAAT CTGGAGCGCG ACGCCGGAAA TCGGTCGGTA 
ACCAAGTTGG CTAAGGAGCA TTCATTGCTC GAAGCTCGAA TTGCAGCTTT AATTGAAGAA 
AAACGTCAGC TCCAATCAAT GCTTGACCAA AAAGACGCCA ACTACTCTCA CAAGCGAAAA 
CTCTTAGAAT CCCAAATCCA GTTGCTCCGC GAGCAACTCG AAGCCGAACG CCGAAAGCGT 
ACAAAGGGAG TTGTGGCGAC TGGTCCCACC GTTAGCCGAC GGGGCGTTCA GCACACATCT 
GCCTTTAGGC ACACCATCGA GCGTCATCGG TCTCTGTCCC AGTCTAGTGA ACGGACAATT 
CTTCAGGAAC GATACCTTGA ATATGTATAT ACAGGAGATC GTACTCCAGC AATACAAATG 
ATCAACACAC CGCCAATCTC TCCATTATCA CATTCTGGCT CTTTCAATTC GGGTACTCTT 
ATTATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_494820.3
CDS	1..5829
Translation	MKSAASSEDVVRVTEDLSECRNRLDAGIEENRRNRQVIQDINDQLQRFRQRANAESIESFNLTPSPDVTLSSLAHPGLTHLHNQTNISMPSLTIDIPLNSNAMISSSRTPNYAINGLRNRHKSLVGHRYRSTSPIGDYGRHRSSPRVLAHYNLDGADIGVGEENLDELFAKLKEELFKNNTLEEVNEMLREENDAALAANEHLRVDATNLSRQLQQLQQQQHTESMRFRSENTRYRNQTETQHRKLISLWKEFTAVKRQLHELRTTTANDLDRQLTEFTRCATLMRKAIRHAEQKNLDQKEQMKREKDDVLDETLRQLNSVTENYMKSEEKANERQRDLKRKEDECRKLREQNDELSDILEQLSKMAHEMAGGRGRNETPMDVARKMRKLLTTKNGEIDESREAAKQAEKERDRAKKDLEKEEKRRKDDREAERKRSSVYSQREHDLKKLDDELRKASEKIRNLEEQRESQEKLTISVQNSLNEAHRQHKQFIEELMIRHREELKEREDSHEEALRSKDTEERSRFEKERSEREKIRRESDELRETQRSLKGDVAAMKTDLDDKTLRLDMLETERDELKKKLETEREQADQRDLEIAECRAKLDEMAEKEAELRKELAEFQAIITAMEGEGKLNQEQFLESKNELNTLTDQIESLNSEVENKNEEIRNLMATLQEKEVHIQNVRTSSHQLTATYEEANGEIDILKAELTRLHEQVNERTRQISEANEKYDDAARKNDALLEDVATWQEKYEQLKMELEEMNRRGQEKEREEADLRALLDDLRGNFDKLTNELKQKGVTVDSLNEEISSLKEQLNKSEKERKEELLRMEELEQKNEAEMKEEYEVKLQLAEKDRQGVENFGKECEARMNELTKIHEMLMEEHDQLKVDHLHTEEEVERLKEKMRKELEKLNEQNDGDRAEWSNERNRLESSKNEAVTELQERVQKLEDVVKEKEDKEIALRRDLEDSHEKSRDLDDKLRKMELTDEEKEEDRKKEQKTLNEERMKLMEQKEEAMLVATKHATTIDQQTRRISVLEGDVEKLTAGIAERESSINALESNTMELISKLETTEAELEKLKDELAVMLKQNSELKNGKEGLSEKWNEERKKIQDLADQLREANKVVHNMRMKNVNLEEKKNELDQNVTDLTNKVRQLEIQLMDKAAKNEVSGDLLRKMEHDAQSMLKQAQNEQFRLTDLEKVRKALQDENQRLVNDLATVKAAFEVKRETSKSAISDILDKYRSAEEKANKGELDNQRLRSDLATVTLKLERQELKAKDSDNRLRDSQKRFEEVQSKLANLQKSAVESLQNPMSSNSRQNRSIYVDIPRAASSIGLNENSDEVPLRSSPSVRFADSSQNMQRAVDSMDVSSSVGVTLRFLKERIEQLEADNADLSDALEKAKDELRQRNEKLADRQMVIERVERQLVHITEERNTIENRMTSQRQMYLTNEESSRSREHEIRSMKARISTLELHLREKESKLAHLRKEIEVLHGQLHDALESKEKATGLVGVQDSKHRDLEEQLDRANRERELAIGKQRRTLAENENLFRKLEQLEKEREQLMREITDERRLNERNRTSLEELRVSERTWKSAMTTAKKPAEEQERAVQEQRRWEESNHEMTNRNTALTKECDRLRVEMRDQLNRMNGINLRSVDFERKNEELSSKLIVMQNTVTAMKKFEEEWKRLEAEMRAELKILRKEKLMQTAEIEDLKRKSFRSDTEKKEIEGIRVRLEREISALKRHVDALEEEKGKTEKAVRETMNERRAIDKSLASMERENQQLYRNCAQLQAQIQNLERDAGNRSVTKLAKEHSLLEARIAALIEEKRQLQSMLDQKDANYSHKRKLLESQIQLLREQLEAERRKRTKGVVATGPTVSRRGVQHTSAFRHTIERHRSLSQSSERTILQERYLEYVYTGDRTPAIQMINTPPISPLSHSGSFNSGTLIM

Target ORF information:

RefSeq Version	NM_062419.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Rootletin (che-10), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_062419.5

ORF Insert Sequence:

ATGAAATCGG CGGCAAGTTC GGAGGATGTC GTTCGTGTCA CTGAAGATCT ATCCGAATGC 
CGAAATCGGT TGGATGCTGG GATTGAGGAG AATCGGAGGA ATCGGCAGGT TATCCAGGAT 
ATTAATGATC AGCTCCAACG CTTCCGCCAA AGAGCAAACG CAGAATCAAT CGAGTCTTTC 
AATCTGACCC CCAGCCCCGA TGTGACATTA TCATCACTTG CTCATCCAGG ACTCACACAT 
CTTCACAATC AGACAAACAT TTCCATGCCT TCTCTAACCA TTGACATTCC GTTGAACTCC 
AATGCCATGA TTAGTTCATC TAGAACCCCA AACTATGCGA TTAATGGGTT AAGAAATCGA 
CACAAAAGTC TAGTAGGGCA TAGGTATCGG TCGACGAGCC CGATCGGAGA TTATGGAAGA 
CATCGAAGCA GTCCAAGAGT TTTGGCTCAT TATAATTTGG ATGGTGCAGA TATTGGAGTT 
GGAGAGGAGA ATTTGGACGA ACTTTTTGCA AAGTTGAAAG AGGAATTGTT CAAAAACAAC 
ACACTGGAAG AGGTGAATGA GATGCTCCGA GAAGAAAATG ATGCAGCACT AGCTGCAAAT 
GAGCATTTAA GAGTTGATGC TACTAATTTG AGCAGACAAC TACAGCAATT ACAACAGCAG 
CAGCATACAG AGTCGATGAG ATTTCGGTCG GAGAATACGC GCTATCGAAA TCAAACGGAG 
ACCCAACACA GAAAGCTGAT CAGTCTTTGG AAGGAGTTCA CCGCTGTCAA GCGTCAGCTC 
CACGAGTTGC GCACAACCAC CGCCAACGAT TTGGACAGAC AATTGACCGA ATTCACGCGG 
TGTGCAACAC TTATGCGAAA AGCCATTCGC CACGCCGAGC AAAAGAATCT CGACCAGAAG 
GAACAAATGA AACGAGAAAA GGACGATGTA CTCGATGAGA CACTTCGTCA GCTCAACTCT 
GTCACTGAGA ATTATATGAA ATCAGAAGAA AAAGCAAACG AACGGCAGCG AGATTTGAAG 
AGAAAAGAGG ACGAATGCAG GAAGCTCCGC GAGCAAAACG ACGAGCTCTC GGATATTCTC 
GAACAACTTT CCAAAATGGC TCACGAAATG GCTGGAGGCA GAGGAAGAAA CGAAACACCA 
ATGGATGTGG CACGTAAAAT GCGGAAGCTG CTGACCACCA AGAACGGGGA AATCGATGAA 
TCAAGGGAAG CAGCGAAGCA GGCAGAAAAG GAAAGAGATC GTGCAAAGAA GGATTTGGAA 
AAAGAAGAGA AACGAAGAAA AGATGACAGA GAAGCAGAGA GAAAACGATC AAGTGTCTAT 
TCACAGAGAG AACATGACCT GAAAAAGTTG GATGATGAGT TGAGGAAGGC CAGTGAAAAG 
ATCCGGAATC TCGAGGAGCA ACGGGAGAGC CAGGAAAAAC TCACCATATC AGTTCAGAAC 
TCATTGAATG AAGCACACAG GCAACACAAA CAGTTTATTG AGGAATTGAT GATTCGGCAT 
CGAGAAGAAC TGAAAGAGCG GGAGGATTCT CACGAGGAAG CGTTACGTTC CAAAGATACC 
GAGGAAAGAT CTCGATTTGA AAAAGAACGT TCTGAACGGG AAAAGATTAG ACGAGAAAGT 
GATGAGCTCA GAGAGACGCA GAGAAGTTTA AAGGGTGACG TGGCGGCGAT GAAGACAGAT 
TTGGATGATA AGACTTTAAG GCTTGACATG CTCGAAACAG AACGGGATGA GCTCAAAAAG 
AAACTGGAAA CCGAAAGAGA GCAGGCTGAT CAGAGAGACT TGGAGATAGC AGAGTGTCGA 
GCAAAGCTTG ATGAGATGGC TGAGAAGGAA GCGGAGCTCC GGAAAGAGCT CGCAGAGTTC 
CAGGCAATTA TCACTGCGAT GGAAGGAGAA GGAAAGCTGA ATCAAGAACA GTTTTTGGAG 
AGTAAGAATG AGTTGAACAC GCTGACAGAT CAAATTGAAT CTTTGAATAG TGAGGTGGAG 
AATAAGAATG AGGAAATTCG AAATTTGATG GCTACACTTC AAGAAAAAGA AGTTCACATT 
CAAAACGTCC GAACATCAAG TCATCAGTTG ACTGCCACAT ACGAAGAGGC TAACGGGGAA 
ATCGATATTT TGAAGGCTGA ACTTACCCGG CTCCACGAGC AAGTCAATGA GAGGACTCGA 
CAGATCTCCG AAGCTAATGA GAAGTATGAT GATGCAGCTC GGAAGAATGA CGCATTGCTC 
GAAGATGTCG CCACGTGGCA AGAGAAGTAT GAGCAGTTGA AGATGGAGCT GGAAGAAATG 
AACAGAAGAG GACAGGAGAA GGAACGAGAA GAAGCAGATC TTCGAGCATT ACTTGATGAT 
TTACGTGGAA ACTTTGATAA GTTGACTAAT GAGCTGAAGC AGAAGGGTGT TACTGTTGAT 
AGCTTGAATG AAGAAATTTC ATCGTTGAAA GAACAATTAA ACAAGTCAGA AAAGGAGAGA 
AAAGAAGAAC TGTTAAGGAT GGAAGAGCTT GAACAGAAAA ATGAAGCGGA AATGAAAGAA 
GAGTATGAAG TAAAGTTACA ATTGGCTGAG AAGGATCGTC AAGGAGTTGA GAATTTTGGA 
AAAGAATGTG AAGCGAGAAT GAATGAGCTC ACGAAGATCC ACGAGATGCT AATGGAAGAG 
CACGATCAGT TGAAAGTGGA TCATCTGCAC ACGGAAGAAG AAGTGGAGAG GTTGAAAGAG 
AAAATGAGAA AGGAATTGGA GAAGTTGAAT GAGCAGAATG ATGGTGATCG GGCGGAATGG 
AGTAATGAAA GGAATCGGCT GGAATCATCC AAAAACGAAG CAGTTACGGA ACTTCAAGAA 
CGAGTGCAGA AGCTTGAGGA TGTGGTGAAG GAGAAAGAAG ATAAGGAAAT TGCTCTTCGT 
AGGGATTTAG AAGACAGCCA TGAGAAATCA AGAGATCTTG ATGACAAGCT TCGAAAAATG 
GAGTTGACAG ATGAGGAGAA AGAGGAAGAT CGGAAGAAGG AACAGAAGAC ATTGAATGAA 
GAGAGGATGA AACTCATGGA ACAGAAAGAA GAAGCAATGC TTGTTGCTAC AAAACATGCA 
ACAACGATTG ATCAACAGAC TCGAAGAATT TCAGTGTTGG AGGGTGACGT GGAAAAGCTG 
ACGGCTGGAA TTGCGGAGAG AGAAAGTTCG ATTAATGCTT TGGAATCGAA TACAATGGAG 
CTGATATCCA AGCTGGAAAC TACTGAAGCA GAACTTGAAA AGCTGAAAGA CGAGCTTGCA 
GTCATGCTGA AACAAAACTC GGAACTCAAA AATGGAAAAG AGGGGTTGAG TGAGAAGTGG 
AATGAGGAGA GGAAGAAGAT CCAAGACTTG GCAGATCAAT TGAGAGAGGC TAACAAAGTT 
GTTCATAATA TGAGAATGAA GAATGTTAAT CTAGAAGAAA AGAAGAATGA ATTGGATCAG 
AATGTGACTG ATTTGACCAA TAAAGTTCGA CAATTAGAGA TTCAGTTGAT GGATAAAGCT 
GCGAAGAACG AGGTTTCAGG AGATTTGCTC AGAAAGATGG AGCATGATGC TCAGAGTATG 
CTGAAGCAAG CACAGAATGA GCAGTTTAGA CTAACTGATC TTGAAAAAGT TCGAAAAGCT 
CTTCAAGATG AGAATCAACG GCTTGTAAAT GATCTGGCAA CAGTGAAAGC TGCATTTGAA 
GTCAAGAGAG AAACGAGCAA ATCTGCAATC AGTGACATTT TGGATAAGTA CCGGAGTGCT 
GAGGAGAAGG CCAATAAGGG TGAATTGGAT AATCAGAGGC TTAGAAGTGA TCTGGCTACT 
GTGACATTAA AACTGGAACG TCAAGAACTG AAAGCAAAAG ATTCTGATAA TCGACTGAGA 
GATAGCCAAA AACGATTCGA AGAAGTTCAA TCAAAACTTG CAAATCTTCA GAAATCAGCA 
GTAGAATCCT TACAAAATCC TATGTCTTCG AATTCCCGCC AAAATCGGTC AATCTATGTT 
GACATTCCAC GTGCAGCTTC TTCGATCGGC CTCAATGAGA ATTCTGATGA AGTCCCGCTT 
CGCAGTTCCC CCTCAGTCCG TTTTGCAGAC AGCTCACAGA ATATGCAAAG AGCAGTTGAC 
TCTATGGATG TGAGTTCTTC AGTTGGAGTT ACACTTCGGT TTTTGAAAGA AAGAATCGAA 
CAGTTGGAAG CAGACAATGC AGATTTATCA GATGCTCTGG AAAAGGCAAA AGATGAGCTG 
AGGCAGAGAA ATGAGAAGCT GGCGGATAGG CAAATGGTTA TTGAGAGAGT TGAGAGACAA 
TTGGTTCATA TTACTGAAGA ACGCAATACA ATTGAAAATC GAATGACAAG TCAGCGTCAA 
ATGTATCTCA CAAATGAGGA ATCATCACGA TCAAGAGAAC ATGAAATCAG AAGTATGAAA 
GCCAGAATTT CAACTTTAGA GCTTCATCTT CGAGAAAAAG AATCTAAACT TGCACATCTA 
CGAAAAGAAA TTGAAGTACT TCATGGTCAG CTTCATGATG CTCTCGAATC AAAGGAAAAG 
GCTACTGGAT TGGTTGGAGT TCAAGATTCA AAGCATCGAG ACTTAGAAGA ACAACTCGAT 
CGAGCAAATC GTGAACGAGA ATTGGCAATT GGGAAGCAGA GAAGAACTCT TGCAGAGAAC 
GAGAATCTGT TCCGGAAGCT TGAACAATTG GAAAAGGAAA GAGAGCAGCT TATGCGAGAG 
ATCACAGATG AACGGAGGCT CAACGAGCGG AATCGCACAA GTCTTGAAGA ACTTCGAGTC 
TCTGAAAGAA CATGGAAATC AGCCATGACA ACTGCCAAAA AACCAGCTGA GGAGCAGGAA 
CGGGCGGTGC AGGAGCAACG GAGATGGGAA GAGTCAAATC ATGAAATGAC GAATAGAAAT 
ACTGCATTGA CAAAAGAATG TGATAGATTA CGAGTTGAAA TGAGAGATCA ACTCAACAGA 
ATGAACGGGA TCAATTTGAG ATCAGTAGAT TTCGAGAGAA AGAATGAAGA GCTTTCAAGT 
AAACTGATTG TTATGCAAAA TACAGTAACT GCGATGAAAA AGTTCGAGGA AGAATGGAAG 
AGGTTGGAGG CGGAGATGAG AGCAGAACTT AAGATTTTGA GAAAAGAAAA GCTCATGCAA 
ACTGCTGAAA TTGAGGATTT AAAGAGGAAA AGCTTCAGAA GTGACACTGA AAAGAAGGAG 
ATCGAGGGTA TTCGAGTAAG GTTGGAAAGG GAAATCAGTG CACTTAAGAG ACATGTTGAT 
GCGCTAGAAG AAGAGAAGGG AAAAACTGAA AAGGCCGTTC GAGAAACCAT GAACGAACGT 
CGTGCCATCG ACAAATCTCT CGCGTCAATG GAACGAGAAA ATCAACAGTT GTATAGGAAT 
TGTGCTCAAC TTCAAGCTCA AATTCAGAAT CTGGAGCGCG ACGCCGGAAA TCGGTCGGTA 
ACCAAGTTGG CTAAGGAGCA TTCATTGCTC GAAGCTCGAA TTGCAGCTTT AATTGAAGAA 
AAACGTCAGC TCCAATCAAT GCTTGACCAA AAAGACGCCA ACTACTCTCA CAAGCGAAAA 
CTCTTAGAAT CCCAAATCCA GTTGCTCCGC GAGCAACTCG AAGCCGAACG CCGAAAGCGT 
ACAAAGGGAG TTGTGGCGAC TGGTCCCACC GTTAGCCGAC GGGGCGTTCA GCACACATCT 
GCCTTTAGGC ACACCATCGA GCGTCATCGG TCTCTGTCCC AGTCTAGTGA ACGGACAATT 
CTTCAGGAAC GATACCTTGA ATATGTATAT ACAGGAGATC GTACTCCAGC AATACAAATG 
ATCAACACAC CGCCAATCTC TCCATTATCA CATTCTGGCT CTTTCAATTC GGGTACTCTT 
ATTATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

che-10 ( NM_062419.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_494820.3 Caenorhabditis elegans Rootletin (che-10), mRNA.

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CloneID	OCa339204
Clone ID Related Accession (Same CDS sequence)	NM_062419.5 , NM_062419.6
Accession Version	NM_062419.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Rootletin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_062419.5.

ATGAAATCGG CGGCAAGTTC GGAGGATGTC GTTCGTGTCA CTGAAGATCT ATCCGAATGC 
CGAAATCGGT TGGATGCTGG GATTGAGGAG AATCGGAGGA ATCGGCAGGT TATCCAGGAT 
ATTAATGATC AGCTCCAACG CTTCCGCCAA AGAGCAAACG CAGAATCAAT CGAGTCTTTC 
AATCTGACCC CCAGCCCCGA TGTGACATTA TCATCACTTG CTCATCCAGG ACTCACACAT 
CTTCACAATC AGACAAACAT TTCCATGCCT TCTCTAACCA TTGACATTCC GTTGAACTCC 
AATGCCATGA TTAGTTCATC TAGAACCCCA AACTATGCGA TTAATGGGTT AAGAAATCGA 
CACAAAAGTC TAGTAGGGCA TAGGTATCGG TCGACGAGCC CGATCGGAGA TTATGGAAGA 
CATCGAAGCA GTCCAAGAGT TTTGGCTCAT TATAATTTGG ATGGTGCAGA TATTGGAGTT 
GGAGAGGAGA ATTTGGACGA ACTTTTTGCA AAGTTGAAAG AGGAATTGTT CAAAAACAAC 
ACACTGGAAG AGGTGAATGA GATGCTCCGA GAAGAAAATG ATGCAGCACT AGCTGCAAAT 
GAGCATTTAA GAGTTGATGC TACTAATTTG AGCAGACAAC TACAGCAATT ACAACAGCAG 
CAGCATACAG AGTCGATGAG ATTTCGGTCG GAGAATACGC GCTATCGAAA TCAAACGGAG 
ACCCAACACA GAAAGCTGAT CAGTCTTTGG AAGGAGTTCA CCGCTGTCAA GCGTCAGCTC 
CACGAGTTGC GCACAACCAC CGCCAACGAT TTGGACAGAC AATTGACCGA ATTCACGCGG 
TGTGCAACAC TTATGCGAAA AGCCATTCGC CACGCCGAGC AAAAGAATCT CGACCAGAAG 
GAACAAATGA AACGAGAAAA GGACGATGTA CTCGATGAGA CACTTCGTCA GCTCAACTCT 
GTCACTGAGA ATTATATGAA ATCAGAAGAA AAAGCAAACG AACGGCAGCG AGATTTGAAG 
AGAAAAGAGG ACGAATGCAG GAAGCTCCGC GAGCAAAACG ACGAGCTCTC GGATATTCTC 
GAACAACTTT CCAAAATGGC TCACGAAATG GCTGGAGGCA GAGGAAGAAA CGAAACACCA 
ATGGATGTGG CACGTAAAAT GCGGAAGCTG CTGACCACCA AGAACGGGGA AATCGATGAA 
TCAAGGGAAG CAGCGAAGCA GGCAGAAAAG GAAAGAGATC GTGCAAAGAA GGATTTGGAA 
AAAGAAGAGA AACGAAGAAA AGATGACAGA GAAGCAGAGA GAAAACGATC AAGTGTCTAT 
TCACAGAGAG AACATGACCT GAAAAAGTTG GATGATGAGT TGAGGAAGGC CAGTGAAAAG 
ATCCGGAATC TCGAGGAGCA ACGGGAGAGC CAGGAAAAAC TCACCATATC AGTTCAGAAC 
TCATTGAATG AAGCACACAG GCAACACAAA CAGTTTATTG AGGAATTGAT GATTCGGCAT 
CGAGAAGAAC TGAAAGAGCG GGAGGATTCT CACGAGGAAG CGTTACGTTC CAAAGATACC 
GAGGAAAGAT CTCGATTTGA AAAAGAACGT TCTGAACGGG AAAAGATTAG ACGAGAAAGT 
GATGAGCTCA GAGAGACGCA GAGAAGTTTA AAGGGTGACG TGGCGGCGAT GAAGACAGAT 
TTGGATGATA AGACTTTAAG GCTTGACATG CTCGAAACAG AACGGGATGA GCTCAAAAAG 
AAACTGGAAA CCGAAAGAGA GCAGGCTGAT CAGAGAGACT TGGAGATAGC AGAGTGTCGA 
GCAAAGCTTG ATGAGATGGC TGAGAAGGAA GCGGAGCTCC GGAAAGAGCT CGCAGAGTTC 
CAGGCAATTA TCACTGCGAT GGAAGGAGAA GGAAAGCTGA ATCAAGAACA GTTTTTGGAG 
AGTAAGAATG AGTTGAACAC GCTGACAGAT CAAATTGAAT CTTTGAATAG TGAGGTGGAG 
AATAAGAATG AGGAAATTCG AAATTTGATG GCTACACTTC AAGAAAAAGA AGTTCACATT 
CAAAACGTCC GAACATCAAG TCATCAGTTG ACTGCCACAT ACGAAGAGGC TAACGGGGAA 
ATCGATATTT TGAAGGCTGA ACTTACCCGG CTCCACGAGC AAGTCAATGA GAGGACTCGA 
CAGATCTCCG AAGCTAATGA GAAGTATGAT GATGCAGCTC GGAAGAATGA CGCATTGCTC 
GAAGATGTCG CCACGTGGCA AGAGAAGTAT GAGCAGTTGA AGATGGAGCT GGAAGAAATG 
AACAGAAGAG GACAGGAGAA GGAACGAGAA GAAGCAGATC TTCGAGCATT ACTTGATGAT 
TTACGTGGAA ACTTTGATAA GTTGACTAAT GAGCTGAAGC AGAAGGGTGT TACTGTTGAT 
AGCTTGAATG AAGAAATTTC ATCGTTGAAA GAACAATTAA ACAAGTCAGA AAAGGAGAGA 
AAAGAAGAAC TGTTAAGGAT GGAAGAGCTT GAACAGAAAA ATGAAGCGGA AATGAAAGAA 
GAGTATGAAG TAAAGTTACA ATTGGCTGAG AAGGATCGTC AAGGAGTTGA GAATTTTGGA 
AAAGAATGTG AAGCGAGAAT GAATGAGCTC ACGAAGATCC ACGAGATGCT AATGGAAGAG 
CACGATCAGT TGAAAGTGGA TCATCTGCAC ACGGAAGAAG AAGTGGAGAG GTTGAAAGAG 
AAAATGAGAA AGGAATTGGA GAAGTTGAAT GAGCAGAATG ATGGTGATCG GGCGGAATGG 
AGTAATGAAA GGAATCGGCT GGAATCATCC AAAAACGAAG CAGTTACGGA ACTTCAAGAA 
CGAGTGCAGA AGCTTGAGGA TGTGGTGAAG GAGAAAGAAG ATAAGGAAAT TGCTCTTCGT 
AGGGATTTAG AAGACAGCCA TGAGAAATCA AGAGATCTTG ATGACAAGCT TCGAAAAATG 
GAGTTGACAG ATGAGGAGAA AGAGGAAGAT CGGAAGAAGG AACAGAAGAC ATTGAATGAA 
GAGAGGATGA AACTCATGGA ACAGAAAGAA GAAGCAATGC TTGTTGCTAC AAAACATGCA 
ACAACGATTG ATCAACAGAC TCGAAGAATT TCAGTGTTGG AGGGTGACGT GGAAAAGCTG 
ACGGCTGGAA TTGCGGAGAG AGAAAGTTCG ATTAATGCTT TGGAATCGAA TACAATGGAG 
CTGATATCCA AGCTGGAAAC TACTGAAGCA GAACTTGAAA AGCTGAAAGA CGAGCTTGCA 
GTCATGCTGA AACAAAACTC GGAACTCAAA AATGGAAAAG AGGGGTTGAG TGAGAAGTGG 
AATGAGGAGA GGAAGAAGAT CCAAGACTTG GCAGATCAAT TGAGAGAGGC TAACAAAGTT 
GTTCATAATA TGAGAATGAA GAATGTTAAT CTAGAAGAAA AGAAGAATGA ATTGGATCAG 
AATGTGACTG ATTTGACCAA TAAAGTTCGA CAATTAGAGA TTCAGTTGAT GGATAAAGCT 
GCGAAGAACG AGGTTTCAGG AGATTTGCTC AGAAAGATGG AGCATGATGC TCAGAGTATG 
CTGAAGCAAG CACAGAATGA GCAGTTTAGA CTAACTGATC TTGAAAAAGT TCGAAAAGCT 
CTTCAAGATG AGAATCAACG GCTTGTAAAT GATCTGGCAA CAGTGAAAGC TGCATTTGAA 
GTCAAGAGAG AAACGAGCAA ATCTGCAATC AGTGACATTT TGGATAAGTA CCGGAGTGCT 
GAGGAGAAGG CCAATAAGGG TGAATTGGAT AATCAGAGGC TTAGAAGTGA TCTGGCTACT 
GTGACATTAA AACTGGAACG TCAAGAACTG AAAGCAAAAG ATTCTGATAA TCGACTGAGA 
GATAGCCAAA AACGATTCGA AGAAGTTCAA TCAAAACTTG CAAATCTTCA GAAATCAGCA 
GTAGAATCCT TACAAAATCC TATGTCTTCG AATTCCCGCC AAAATCGGTC AATCTATGTT 
GACATTCCAC GTGCAGCTTC TTCGATCGGC CTCAATGAGA ATTCTGATGA AGTCCCGCTT 
CGCAGTTCCC CCTCAGTCCG TTTTGCAGAC AGCTCACAGA ATATGCAAAG AGCAGTTGAC 
TCTATGGATG TGAGTTCTTC AGTTGGAGTT ACACTTCGGT TTTTGAAAGA AAGAATCGAA 
CAGTTGGAAG CAGACAATGC AGATTTATCA GATGCTCTGG AAAAGGCAAA AGATGAGCTG 
AGGCAGAGAA ATGAGAAGCT GGCGGATAGG CAAATGGTTA TTGAGAGAGT TGAGAGACAA 
TTGGTTCATA TTACTGAAGA ACGCAATACA ATTGAAAATC GAATGACAAG TCAGCGTCAA 
ATGTATCTCA CAAATGAGGA ATCATCACGA TCAAGAGAAC ATGAAATCAG AAGTATGAAA 
GCCAGAATTT CAACTTTAGA GCTTCATCTT CGAGAAAAAG AATCTAAACT TGCACATCTA 
CGAAAAGAAA TTGAAGTACT TCATGGTCAG CTTCATGATG CTCTCGAATC AAAGGAAAAG 
GCTACTGGAT TGGTTGGAGT TCAAGATTCA AAGCATCGAG ACTTAGAAGA ACAACTCGAT 
CGAGCAAATC GTGAACGAGA ATTGGCAATT GGGAAGCAGA GAAGAACTCT TGCAGAGAAC 
GAGAATCTGT TCCGGAAGCT TGAACAATTG GAAAAGGAAA GAGAGCAGCT TATGCGAGAG 
ATCACAGATG AACGGAGGCT CAACGAGCGG AATCGCACAA GTCTTGAAGA ACTTCGAGTC 
TCTGAAAGAA CATGGAAATC AGCCATGACA ACTGCCAAAA AACCAGCTGA GGAGCAGGAA 
CGGGCGGTGC AGGAGCAACG GAGATGGGAA GAGTCAAATC ATGAAATGAC GAATAGAAAT 
ACTGCATTGA CAAAAGAATG TGATAGATTA CGAGTTGAAA TGAGAGATCA ACTCAACAGA 
ATGAACGGGA TCAATTTGAG ATCAGTAGAT TTCGAGAGAA AGAATGAAGA GCTTTCAAGT 
AAACTGATTG TTATGCAAAA TACAGTAACT GCGATGAAAA AGTTCGAGGA AGAATGGAAG 
AGGTTGGAGG CGGAGATGAG AGCAGAACTT AAGATTTTGA GAAAAGAAAA GCTCATGCAA 
ACTGCTGAAA TTGAGGATTT AAAGAGGAAA AGCTTCAGAA GTGACACTGA AAAGAAGGAG 
ATCGAGGGTA TTCGAGTAAG GTTGGAAAGG GAAATCAGTG CACTTAAGAG ACATGTTGAT 
GCGCTAGAAG AAGAGAAGGG AAAAACTGAA AAGGCCGTTC GAGAAACCAT GAACGAACGT 
CGTGCCATCG ACAAATCTCT CGCGTCAATG GAACGAGAAA ATCAACAGTT GTATAGGAAT 
TGTGCTCAAC TTCAAGCTCA AATTCAGAAT CTGGAGCGCG ACGCCGGAAA TCGGTCGGTA 
ACCAAGTTGG CTAAGGAGCA TTCATTGCTC GAAGCTCGAA TTGCAGCTTT AATTGAAGAA 
AAACGTCAGC TCCAATCAAT GCTTGACCAA AAAGACGCCA ACTACTCTCA CAAGCGAAAA 
CTCTTAGAAT CCCAAATCCA GTTGCTCCGC GAGCAACTCG AAGCCGAACG CCGAAAGCGT 
ACAAAGGGAG TTGTGGCGAC TGGTCCCACC GTTAGCCGAC GGGGCGTTCA GCACACATCT 
GCCTTTAGGC ACACCATCGA GCGTCATCGG TCTCTGTCCC AGTCTAGTGA ACGGACAATT 
CTTCAGGAAC GATACCTTGA ATATGTATAT ACAGGAGATC GTACTCCAGC AATACAAATG 
ATCAACACAC CGCCAATCTC TCCATTATCA CATTCTGGCT CTTTCAATTC GGGTACTCTT 
ATTATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_494820.3
CDS	44..5872
Translation	MKSAASSEDVVRVTEDLSECRNRLDAGIEENRRNRQVIQDINDQLQRFRQRANAESIESFNLTPSPDVTLSSLAHPGLTHLHNQTNISMPSLTIDIPLNSNAMISSSRTPNYAINGLRNRHKSLVGHRYRSTSPIGDYGRHRSSPRVLAHYNLDGADIGVGEENLDELFAKLKEELFKNNTLEEVNEMLREENDAALAANEHLRVDATNLSRQLQQLQQQQHTESMRFRSENTRYRNQTETQHRKLISLWKEFTAVKRQLHELRTTTANDLDRQLTEFTRCATLMRKAIRHAEQKNLDQKEQMKREKDDVLDETLRQLNSVTENYMKSEEKANERQRDLKRKEDECRKLREQNDELSDILEQLSKMAHEMAGGRGRNETPMDVARKMRKLLTTKNGEIDESREAAKQAEKERDRAKKDLEKEEKRRKDDREAERKRSSVYSQREHDLKKLDDELRKASEKIRNLEEQRESQEKLTISVQNSLNEAHRQHKQFIEELMIRHREELKEREDSHEEALRSKDTEERSRFEKERSEREKIRRESDELRETQRSLKGDVAAMKTDLDDKTLRLDMLETERDELKKKLETEREQADQRDLEIAECRAKLDEMAEKEAELRKELAEFQAIITAMEGEGKLNQEQFLESKNELNTLTDQIESLNSEVENKNEEIRNLMATLQEKEVHIQNVRTSSHQLTATYEEANGEIDILKAELTRLHEQVNERTRQISEANEKYDDAARKNDALLEDVATWQEKYEQLKMELEEMNRRGQEKEREEADLRALLDDLRGNFDKLTNELKQKGVTVDSLNEEISSLKEQLNKSEKERKEELLRMEELEQKNEAEMKEEYEVKLQLAEKDRQGVENFGKECEARMNELTKIHEMLMEEHDQLKVDHLHTEEEVERLKEKMRKELEKLNEQNDGDRAEWSNERNRLESSKNEAVTELQERVQKLEDVVKEKEDKEIALRRDLEDSHEKSRDLDDKLRKMELTDEEKEEDRKKEQKTLNEERMKLMEQKEEAMLVATKHATTIDQQTRRISVLEGDVEKLTAGIAERESSINALESNTMELISKLETTEAELEKLKDELAVMLKQNSELKNGKEGLSEKWNEERKKIQDLADQLREANKVVHNMRMKNVNLEEKKNELDQNVTDLTNKVRQLEIQLMDKAAKNEVSGDLLRKMEHDAQSMLKQAQNEQFRLTDLEKVRKALQDENQRLVNDLATVKAAFEVKRETSKSAISDILDKYRSAEEKANKGELDNQRLRSDLATVTLKLERQELKAKDSDNRLRDSQKRFEEVQSKLANLQKSAVESLQNPMSSNSRQNRSIYVDIPRAASSIGLNENSDEVPLRSSPSVRFADSSQNMQRAVDSMDVSSSVGVTLRFLKERIEQLEADNADLSDALEKAKDELRQRNEKLADRQMVIERVERQLVHITEERNTIENRMTSQRQMYLTNEESSRSREHEIRSMKARISTLELHLREKESKLAHLRKEIEVLHGQLHDALESKEKATGLVGVQDSKHRDLEEQLDRANRERELAIGKQRRTLAENENLFRKLEQLEKEREQLMREITDERRLNERNRTSLEELRVSERTWKSAMTTAKKPAEEQERAVQEQRRWEESNHEMTNRNTALTKECDRLRVEMRDQLNRMNGINLRSVDFERKNEELSSKLIVMQNTVTAMKKFEEEWKRLEAEMRAELKILRKEKLMQTAEIEDLKRKSFRSDTEKKEIEGIRVRLEREISALKRHVDALEEEKGKTEKAVRETMNERRAIDKSLASMERENQQLYRNCAQLQAQIQNLERDAGNRSVTKLAKEHSLLEARIAALIEEKRQLQSMLDQKDANYSHKRKLLESQIQLLREQLEAERRKRTKGVVATGPTVSRRGVQHTSAFRHTIERHRSLSQSSERTILQERYLEYVYTGDRTPAIQMINTPPISPLSHSGSFNSGTLIM

Target ORF information:

RefSeq Version	NM_062419.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Rootletin (che-10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_062419.6

ORF Insert Sequence:

ATGAAATCGG CGGCAAGTTC GGAGGATGTC GTTCGTGTCA CTGAAGATCT ATCCGAATGC 
CGAAATCGGT TGGATGCTGG GATTGAGGAG AATCGGAGGA ATCGGCAGGT TATCCAGGAT 
ATTAATGATC AGCTCCAACG CTTCCGCCAA AGAGCAAACG CAGAATCAAT CGAGTCTTTC 
AATCTGACCC CCAGCCCCGA TGTGACATTA TCATCACTTG CTCATCCAGG ACTCACACAT 
CTTCACAATC AGACAAACAT TTCCATGCCT TCTCTAACCA TTGACATTCC GTTGAACTCC 
AATGCCATGA TTAGTTCATC TAGAACCCCA AACTATGCGA TTAATGGGTT AAGAAATCGA 
CACAAAAGTC TAGTAGGGCA TAGGTATCGG TCGACGAGCC CGATCGGAGA TTATGGAAGA 
CATCGAAGCA GTCCAAGAGT TTTGGCTCAT TATAATTTGG ATGGTGCAGA TATTGGAGTT 
GGAGAGGAGA ATTTGGACGA ACTTTTTGCA AAGTTGAAAG AGGAATTGTT CAAAAACAAC 
ACACTGGAAG AGGTGAATGA GATGCTCCGA GAAGAAAATG ATGCAGCACT AGCTGCAAAT 
GAGCATTTAA GAGTTGATGC TACTAATTTG AGCAGACAAC TACAGCAATT ACAACAGCAG 
CAGCATACAG AGTCGATGAG ATTTCGGTCG GAGAATACGC GCTATCGAAA TCAAACGGAG 
ACCCAACACA GAAAGCTGAT CAGTCTTTGG AAGGAGTTCA CCGCTGTCAA GCGTCAGCTC 
CACGAGTTGC GCACAACCAC CGCCAACGAT TTGGACAGAC AATTGACCGA ATTCACGCGG 
TGTGCAACAC TTATGCGAAA AGCCATTCGC CACGCCGAGC AAAAGAATCT CGACCAGAAG 
GAACAAATGA AACGAGAAAA GGACGATGTA CTCGATGAGA CACTTCGTCA GCTCAACTCT 
GTCACTGAGA ATTATATGAA ATCAGAAGAA AAAGCAAACG AACGGCAGCG AGATTTGAAG 
AGAAAAGAGG ACGAATGCAG GAAGCTCCGC GAGCAAAACG ACGAGCTCTC GGATATTCTC 
GAACAACTTT CCAAAATGGC TCACGAAATG GCTGGAGGCA GAGGAAGAAA CGAAACACCA 
ATGGATGTGG CACGTAAAAT GCGGAAGCTG CTGACCACCA AGAACGGGGA AATCGATGAA 
TCAAGGGAAG CAGCGAAGCA GGCAGAAAAG GAAAGAGATC GTGCAAAGAA GGATTTGGAA 
AAAGAAGAGA AACGAAGAAA AGATGACAGA GAAGCAGAGA GAAAACGATC AAGTGTCTAT 
TCACAGAGAG AACATGACCT GAAAAAGTTG GATGATGAGT TGAGGAAGGC CAGTGAAAAG 
ATCCGGAATC TCGAGGAGCA ACGGGAGAGC CAGGAAAAAC TCACCATATC AGTTCAGAAC 
TCATTGAATG AAGCACACAG GCAACACAAA CAGTTTATTG AGGAATTGAT GATTCGGCAT 
CGAGAAGAAC TGAAAGAGCG GGAGGATTCT CACGAGGAAG CGTTACGTTC CAAAGATACC 
GAGGAAAGAT CTCGATTTGA AAAAGAACGT TCTGAACGGG AAAAGATTAG ACGAGAAAGT 
GATGAGCTCA GAGAGACGCA GAGAAGTTTA AAGGGTGACG TGGCGGCGAT GAAGACAGAT 
TTGGATGATA AGACTTTAAG GCTTGACATG CTCGAAACAG AACGGGATGA GCTCAAAAAG 
AAACTGGAAA CCGAAAGAGA GCAGGCTGAT CAGAGAGACT TGGAGATAGC AGAGTGTCGA 
GCAAAGCTTG ATGAGATGGC TGAGAAGGAA GCGGAGCTCC GGAAAGAGCT CGCAGAGTTC 
CAGGCAATTA TCACTGCGAT GGAAGGAGAA GGAAAGCTGA ATCAAGAACA GTTTTTGGAG 
AGTAAGAATG AGTTGAACAC GCTGACAGAT CAAATTGAAT CTTTGAATAG TGAGGTGGAG 
AATAAGAATG AGGAAATTCG AAATTTGATG GCTACACTTC AAGAAAAAGA AGTTCACATT 
CAAAACGTCC GAACATCAAG TCATCAGTTG ACTGCCACAT ACGAAGAGGC TAACGGGGAA 
ATCGATATTT TGAAGGCTGA ACTTACCCGG CTCCACGAGC AAGTCAATGA GAGGACTCGA 
CAGATCTCCG AAGCTAATGA GAAGTATGAT GATGCAGCTC GGAAGAATGA CGCATTGCTC 
GAAGATGTCG CCACGTGGCA AGAGAAGTAT GAGCAGTTGA AGATGGAGCT GGAAGAAATG 
AACAGAAGAG GACAGGAGAA GGAACGAGAA GAAGCAGATC TTCGAGCATT ACTTGATGAT 
TTACGTGGAA ACTTTGATAA GTTGACTAAT GAGCTGAAGC AGAAGGGTGT TACTGTTGAT 
AGCTTGAATG AAGAAATTTC ATCGTTGAAA GAACAATTAA ACAAGTCAGA AAAGGAGAGA 
AAAGAAGAAC TGTTAAGGAT GGAAGAGCTT GAACAGAAAA ATGAAGCGGA AATGAAAGAA 
GAGTATGAAG TAAAGTTACA ATTGGCTGAG AAGGATCGTC AAGGAGTTGA GAATTTTGGA 
AAAGAATGTG AAGCGAGAAT GAATGAGCTC ACGAAGATCC ACGAGATGCT AATGGAAGAG 
CACGATCAGT TGAAAGTGGA TCATCTGCAC ACGGAAGAAG AAGTGGAGAG GTTGAAAGAG 
AAAATGAGAA AGGAATTGGA GAAGTTGAAT GAGCAGAATG ATGGTGATCG GGCGGAATGG 
AGTAATGAAA GGAATCGGCT GGAATCATCC AAAAACGAAG CAGTTACGGA ACTTCAAGAA 
CGAGTGCAGA AGCTTGAGGA TGTGGTGAAG GAGAAAGAAG ATAAGGAAAT TGCTCTTCGT 
AGGGATTTAG AAGACAGCCA TGAGAAATCA AGAGATCTTG ATGACAAGCT TCGAAAAATG 
GAGTTGACAG ATGAGGAGAA AGAGGAAGAT CGGAAGAAGG AACAGAAGAC ATTGAATGAA 
GAGAGGATGA AACTCATGGA ACAGAAAGAA GAAGCAATGC TTGTTGCTAC AAAACATGCA 
ACAACGATTG ATCAACAGAC TCGAAGAATT TCAGTGTTGG AGGGTGACGT GGAAAAGCTG 
ACGGCTGGAA TTGCGGAGAG AGAAAGTTCG ATTAATGCTT TGGAATCGAA TACAATGGAG 
CTGATATCCA AGCTGGAAAC TACTGAAGCA GAACTTGAAA AGCTGAAAGA CGAGCTTGCA 
GTCATGCTGA AACAAAACTC GGAACTCAAA AATGGAAAAG AGGGGTTGAG TGAGAAGTGG 
AATGAGGAGA GGAAGAAGAT CCAAGACTTG GCAGATCAAT TGAGAGAGGC TAACAAAGTT 
GTTCATAATA TGAGAATGAA GAATGTTAAT CTAGAAGAAA AGAAGAATGA ATTGGATCAG 
AATGTGACTG ATTTGACCAA TAAAGTTCGA CAATTAGAGA TTCAGTTGAT GGATAAAGCT 
GCGAAGAACG AGGTTTCAGG AGATTTGCTC AGAAAGATGG AGCATGATGC TCAGAGTATG 
CTGAAGCAAG CACAGAATGA GCAGTTTAGA CTAACTGATC TTGAAAAAGT TCGAAAAGCT 
CTTCAAGATG AGAATCAACG GCTTGTAAAT GATCTGGCAA CAGTGAAAGC TGCATTTGAA 
GTCAAGAGAG AAACGAGCAA ATCTGCAATC AGTGACATTT TGGATAAGTA CCGGAGTGCT 
GAGGAGAAGG CCAATAAGGG TGAATTGGAT AATCAGAGGC TTAGAAGTGA TCTGGCTACT 
GTGACATTAA AACTGGAACG TCAAGAACTG AAAGCAAAAG ATTCTGATAA TCGACTGAGA 
GATAGCCAAA AACGATTCGA AGAAGTTCAA TCAAAACTTG CAAATCTTCA GAAATCAGCA 
GTAGAATCCT TACAAAATCC TATGTCTTCG AATTCCCGCC AAAATCGGTC AATCTATGTT 
GACATTCCAC GTGCAGCTTC TTCGATCGGC CTCAATGAGA ATTCTGATGA AGTCCCGCTT 
CGCAGTTCCC CCTCAGTCCG TTTTGCAGAC AGCTCACAGA ATATGCAAAG AGCAGTTGAC 
TCTATGGATG TGAGTTCTTC AGTTGGAGTT ACACTTCGGT TTTTGAAAGA AAGAATCGAA 
CAGTTGGAAG CAGACAATGC AGATTTATCA GATGCTCTGG AAAAGGCAAA AGATGAGCTG 
AGGCAGAGAA ATGAGAAGCT GGCGGATAGG CAAATGGTTA TTGAGAGAGT TGAGAGACAA 
TTGGTTCATA TTACTGAAGA ACGCAATACA ATTGAAAATC GAATGACAAG TCAGCGTCAA 
ATGTATCTCA CAAATGAGGA ATCATCACGA TCAAGAGAAC ATGAAATCAG AAGTATGAAA 
GCCAGAATTT CAACTTTAGA GCTTCATCTT CGAGAAAAAG AATCTAAACT TGCACATCTA 
CGAAAAGAAA TTGAAGTACT TCATGGTCAG CTTCATGATG CTCTCGAATC AAAGGAAAAG 
GCTACTGGAT TGGTTGGAGT TCAAGATTCA AAGCATCGAG ACTTAGAAGA ACAACTCGAT 
CGAGCAAATC GTGAACGAGA ATTGGCAATT GGGAAGCAGA GAAGAACTCT TGCAGAGAAC 
GAGAATCTGT TCCGGAAGCT TGAACAATTG GAAAAGGAAA GAGAGCAGCT TATGCGAGAG 
ATCACAGATG AACGGAGGCT CAACGAGCGG AATCGCACAA GTCTTGAAGA ACTTCGAGTC 
TCTGAAAGAA CATGGAAATC AGCCATGACA ACTGCCAAAA AACCAGCTGA GGAGCAGGAA 
CGGGCGGTGC AGGAGCAACG GAGATGGGAA GAGTCAAATC ATGAAATGAC GAATAGAAAT 
ACTGCATTGA CAAAAGAATG TGATAGATTA CGAGTTGAAA TGAGAGATCA ACTCAACAGA 
ATGAACGGGA TCAATTTGAG ATCAGTAGAT TTCGAGAGAA AGAATGAAGA GCTTTCAAGT 
AAACTGATTG TTATGCAAAA TACAGTAACT GCGATGAAAA AGTTCGAGGA AGAATGGAAG 
AGGTTGGAGG CGGAGATGAG AGCAGAACTT AAGATTTTGA GAAAAGAAAA GCTCATGCAA 
ACTGCTGAAA TTGAGGATTT AAAGAGGAAA AGCTTCAGAA GTGACACTGA AAAGAAGGAG 
ATCGAGGGTA TTCGAGTAAG GTTGGAAAGG GAAATCAGTG CACTTAAGAG ACATGTTGAT 
GCGCTAGAAG AAGAGAAGGG AAAAACTGAA AAGGCCGTTC GAGAAACCAT GAACGAACGT 
CGTGCCATCG ACAAATCTCT CGCGTCAATG GAACGAGAAA ATCAACAGTT GTATAGGAAT 
TGTGCTCAAC TTCAAGCTCA AATTCAGAAT CTGGAGCGCG ACGCCGGAAA TCGGTCGGTA 
ACCAAGTTGG CTAAGGAGCA TTCATTGCTC GAAGCTCGAA TTGCAGCTTT AATTGAAGAA 
AAACGTCAGC TCCAATCAAT GCTTGACCAA AAAGACGCCA ACTACTCTCA CAAGCGAAAA 
CTCTTAGAAT CCCAAATCCA GTTGCTCCGC GAGCAACTCG AAGCCGAACG CCGAAAGCGT 
ACAAAGGGAG TTGTGGCGAC TGGTCCCACC GTTAGCCGAC GGGGCGTTCA GCACACATCT 
GCCTTTAGGC ACACCATCGA GCGTCATCGG TCTCTGTCCC AGTCTAGTGA ACGGACAATT 
CTTCAGGAAC GATACCTTGA ATATGTATAT ACAGGAGATC GTACTCCAGC AATACAAATG 
ATCAACACAC CGCCAATCTC TCCATTATCA CATTCTGGCT CTTTCAATTC GGGTACTCTT 
ATTATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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che-10 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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