dog-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	dog-1
Entrez Gene ID	173370
Full Name	Deletions Of G-rich DNA
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_061217.5	NP_493618.1	Deletions Of G-rich DNA
NM_061217.6	NP_493618.1	Helicase ATP-binding domain-containing protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Absence of DOG-1 is not only tolerated but also cooperate to achieve long-term population survival.
Title: Spectrum of variations in dog-1/FANCJ and mdf-1/MAD1 defective Caenorhabditis elegans strains after long-term propagation.

These data provide evidence that the G4 sequence instability in FANCJ(-/-) cells and Fancj/dog1 deficient C. elegans is caused by replication stalling at G-quadruplexes.
Title: FANCJ promotes DNA synthesis through G-quadruplex structures.

analysis of the spectrum of mutational events in the absence of DOG-1/FANCJ in Caenorhabditis elegans
Title: Spectrum of mutational events in the absence of DOG-1/FANCJ in Caenorhabditis elegans.

dog-1 is the Caenorhabditis elegans BRIP1/FANCJ homologue and functions in interstrand cross-link repair.
Title: DOG-1 is the Caenorhabditis elegans BRIP1/FANCJ homologue and functions in interstrand cross-link repair.

absence of DOG-1 leads to replication fork stalling that can be repaired by deletion-free or deletion-prone mechanisms
Title: Homologous recombination is required for genome stability in the absence of DOG-1 in Caenorhabditis elegans.

The following dog-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dog-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa335774	NM_061217.6 Latest version!	Caenorhabditis elegans Helicase ATP-binding domain-containing protein (dog-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OCa335774	NM_061217.5	Caenorhabditis elegans Deletions Of G-rich DNA (dog-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dog-1 ( NM_061217.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_493618.1 Caenorhabditis elegans Deletions Of G-rich DNA (dog-1), partial mRNA.

Price & Availability↑

CloneID	OCa335774
Clone ID Related Accession (Same CDS sequence)	NM_061217.5 , NM_061217.6
Accession Version	NM_061217.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Deletions Of G-rich DNA
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_061217.4. COMPLETENESS: incomplete on both ends.

ATGTCCTCAA GCGATGCATT TTGGAGAATG TTCGCAAACA AAAACAAAGG AAAATCGAAT 
ACCCGATCGG CATTTCAAGT TGTGAAAGAG GAGCAGCCGT CCACATCAAC AGAACCCGAT 
GATAAAAAGC CGTTACACCA TGAAATTGCC GGGGAGATGA TTAAAAATCC GGCAAAAGGA 
AAACGAAAGC GGGTTCAGAT CAAAAACGAC GAGTATGAGC AGCTCATGAT GCTCGGTGTA 
CCGGTACGCG TGCCACGTGG ATTGAGCCTC TACTCGACTC AAAAACTAAT GATCGTGAGA 
ATTCTGACGG CGCTGAAAAA TAGTCAAAAT GTGCTTGGCG AGTCGCCGAC GGGAAGTGGC 
AAAACTATGG CTCTGCTGGC ATCTACGTGT GCATGGCTCA AGCAGTATAT CGATGAGAAG 
CGAGAATCAA AGGAAAAATG TGAAATCCAT GGATTTTCAG GAAAAACACA GGTGGTGTTC 
ACTAACGATA TTGGAATACC GCTAGAATCT AAAGTATATG AAGAGCCACC GGAAGAAGAG 
GAATTCATTG AGCCTGTGCC AGTGAAAAAC GATAAACTTT TCGAGGCACA ATGGGAGCAC 
GATTTCACGC CTCATACACC AGGAGAAACA CCGCCCGTCA CATTGAAAGA AGAGCTGGAG 
CCGGTAAAAA AACCGGAGCC CGTTGAGACG AAATGCACGT GTCTTCCTCG CGTCCGAATC 
TACTACGGCA CCCGAACCCA CAAGCAAATT GCTCAAGTCG TTAAAGAGTT CTCCCGTCTT 
CCATACGCAA AAATTCTGAA ACACACAATT CTCGCAAGCA GAGAACAATC TTGCATCAAT 
CCGGCCGCTC GTAAACACGC AGACATCTCA CAATACTGTA AAGAAGTGAA CAGTGCACAT 
TCGATTGGTT GTTCATTCAA AAGTGCTATG AAACCACGAT TTGAGAAAGC TTTACCGTTA 
AGGGATCATT TAGAGCGAAA TGGAACAGTG GTATTCGATA TGGAAAAAAT TGTCGAAACG 
TTGGCCATAT CATATCCTCA GCTGTGTCCC TACTTCAGCA CAAACCGAAT TCTCACCCAA 
GACGCTGACA TCATTTTCTG TCCATTTTCG TATCTGGTGG ACCCATTGAT TCGAAATTCG 
TCAGATGTTC AGATAAGAAA CTCGATTGTC ATTTTGGATG AAGCTCATAA CATCGAAGAC 
ACGTGCAGAG AAGCGGCAAG CTTCTCGTTC ACCGAGAAAG AGCTCGATGA TTCGATATTG 
TCGCTGAGAA TTAAAAGAAA AGCAGTTGAC ACGGAATTGG ATAAAACCGG CATCGAAGAG 
AAGCTTCTCC ACATTCCAGA TCGAGAAGTG CATCTAAAAG AATTCCGTGG ACATCTTCTC 
TACCTTGAAC ACCTGATCCT TGAGATGCTT CGATGGATCC GACAAGTGTC GACGGAGGCG 
AAAACGCCGG CTAGAGGTGG TCAGGATGGT GTGAAGACAT GCACAATATC GTCTGCAAAT 
CTTTTTACAT CATTGACCGA TCCAGAAAAT GGTGTCGATC TGTTCACACC ACCACTGACA 
CCCAAACATG CAGCAGTGGC GGCGTCTTTC AGTGCGATTA CGAAGCATAA TGAGCCTGAA 
ATGCAGTACT TTGACGCGTT CAAGCCAAGC TCTACAGCGA TAGTCTGCAT AGAGAAATGG 
TTGTATTTTC AGTCATATTT TGGAAATCAG CAGTACCAAT CCACGTATAG GTTAAACATC 
TCTATTGAGC CGATAAATCA AAATGGGCGA TTCAATCATA CTTTTGACGC AGACGTCTCG 
ATGTCGACTT CATTTGGAAA TCCAAGACCA ACAACGACAA GAAGCTCGGC GGGACCCAGA 
AACATGCAGT ACAAGGAGGA GAACGCATGG CTAGCAGATG CTGCAGCTGA CGGAGACGAT 
TGGAAGGATC CGTCAATGTC AGAGACGGGT CATAAACCGA TTTCAGAGGG CTGCAAGACT 
ACGATCAGCT TGTGGTGTAT GAGTCCAGCA TTGTCTTTCT TCGACGCATT CAACGAAACA 
CGTTCTATAG TCCTCGCATC GGGTACTCTG TGCCCAATGG ACACTTTGAA AACCGAATTA 
GGAATGGAAT TCAAGCAACA AGTAGAAGGA GATCAAGTCA TTAATAAGGA TAACATTTTC 
GCAGCGGTTT TGCCAATTGG TCCCTTTGGA AATCGCATTC AGTGCACGTA CCGCAACACG 
TCAGATCCGG AATCTTCATT TTATTGCGAG CTTGGAGCAA TTATCAAGTA TGTATGCAGT 
AACGTGCCAG CAGGAATTCT ATGTTTTCTC CCCAGCTATC GAGTTCTAGA TCAATTAAAA 
CAATGTATGA TCCGTAATTC AACAATGCGA CAAATCGAAA TGAAAAAAGT TGTGCTCTAC 
GAGCCGCGAA GATCCTCAGA ATTAACATCA GTAATGGATC AATTCGATGC GGCGATCTTC 
GATCCGTCTA GATTCGGTGC AAACATCAAC GGATCTCTTA TGTTTGCCGT TTTTCGTGGA 
AAAGTTTCCG AGGGCATTGA TTTTGCCGAT GATCGTGCGC GTGTGGTGAT TTCTGTCGGA 
ATTCCGTATC CAAATGCGAT GGATGATCAA GTGAATGCGA AAAAGTTGTA TAATGATCAG 
AATTCCAAGG AAAAGGGGAT TTTGACTGGC GATGAGTGGT ATACCACGCA GGCCTATCGA 
GCATTGAATC AGGCTTTGGG AAGATGTCTC CGGCACAAAA ACGACTGGGG AGCAATGCTA 
ATGATCGATG AACGCCTAGA ACGTCAAACT GGAAATCTTG TGGGCGGAGC CTCGTCTGCT 
CGTGTCTCAA AATGGATTCG TGCTCAACTG AAAAGCTATC CGAGTTTTAA AGAATTTAAT 
GCGAATTTTC GCGAATTCAT CCAGCGTCGA CACGCTGTTG AGAAAGCAAA AAAAGAGAAT 
TTTTGTGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_493618.1
CDS	1..2952
Translation	MSSSDAFWRMFANKNKGKSNTRSAFQVVKEEQPSTSTEPDDKKPLHHEIAGEMIKNPAKGKRKRVQIKNDEYEQLMMLGVPVRVPRGLSLYSTQKLMIVRILTALKNSQNVLGESPTGSGKTMALLASTCAWLKQYIDEKRESKEKCEIHGFSGKTQVVFTNDIGIPLESKVYEEPPEEEEFIEPVPVKNDKLFEAQWEHDFTPHTPGETPPVTLKEELEPVKKPEPVETKCTCLPRVRIYYGTRTHKQIAQVVKEFSRLPYAKILKHTILASREQSCINPAARKHADISQYCKEVNSAHSIGCSFKSAMKPRFEKALPLRDHLERNGTVVFDMEKIVETLAISYPQLCPYFSTNRILTQDADIIFCPFSYLVDPLIRNSSDVQIRNSIVILDEAHNIEDTCREAASFSFTEKELDDSILSLRIKRKAVDTELDKTGIEEKLLHIPDREVHLKEFRGHLLYLEHLILEMLRWIRQVSTEAKTPARGGQDGVKTCTISSANLFTSLTDPENGVDLFTPPLTPKHAAVAASFSAITKHNEPEMQYFDAFKPSSTAIVCIEKWLYFQSYFGNQQYQSTYRLNISIEPINQNGRFNHTFDADVSMSTSFGNPRPTTTRSSAGPRNMQYKEENAWLADAAADGDDWKDPSMSETGHKPISEGCKTTISLWCMSPALSFFDAFNETRSIVLASGTLCPMDTLKTELGMEFKQQVEGDQVINKDNIFAAVLPIGPFGNRIQCTYRNTSDPESSFYCELGAIIKYVCSNVPAGILCFLPSYRVLDQLKQCMIRNSTMRQIEMKKVVLYEPRRSSELTSVMDQFDAAIFDPSRFGANINGSLMFAVFRGKVSEGIDFADDRARVVISVGIPYPNAMDDQVNAKKLYNDQNSKEKGILTGDEWYTTQAYRALNQALGRCLRHKNDWGAMLMIDERLERQTGNLVGGASSARVSKWIRAQLKSYPSFKEFNANFREFIQRRHAVEKAKKENFCE

Target ORF information:

RefSeq Version	NM_061217.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Deletions Of G-rich DNA (dog-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_061217.5

ORF Insert Sequence:

ATGTCCTCAA GCGATGCATT TTGGAGAATG TTCGCAAACA AAAACAAAGG AAAATCGAAT 
ACCCGATCGG CATTTCAAGT TGTGAAAGAG GAGCAGCCGT CCACATCAAC AGAACCCGAT 
GATAAAAAGC CGTTACACCA TGAAATTGCC GGGGAGATGA TTAAAAATCC GGCAAAAGGA 
AAACGAAAGC GGGTTCAGAT CAAAAACGAC GAGTATGAGC AGCTCATGAT GCTCGGTGTA 
CCGGTACGCG TGCCACGTGG ATTGAGCCTC TACTCGACTC AAAAACTAAT GATCGTGAGA 
ATTCTGACGG CGCTGAAAAA TAGTCAAAAT GTGCTTGGCG AGTCGCCGAC GGGAAGTGGC 
AAAACTATGG CTCTGCTGGC ATCTACGTGT GCATGGCTCA AGCAGTATAT CGATGAGAAG 
CGAGAATCAA AGGAAAAATG TGAAATCCAT GGATTTTCAG GAAAAACACA GGTGGTGTTC 
ACTAACGATA TTGGAATACC GCTAGAATCT AAAGTATATG AAGAGCCACC GGAAGAAGAG 
GAATTCATTG AGCCTGTGCC AGTGAAAAAC GATAAACTTT TCGAGGCACA ATGGGAGCAC 
GATTTCACGC CTCATACACC AGGAGAAACA CCGCCCGTCA CATTGAAAGA AGAGCTGGAG 
CCGGTAAAAA AACCGGAGCC CGTTGAGACG AAATGCACGT GTCTTCCTCG CGTCCGAATC 
TACTACGGCA CCCGAACCCA CAAGCAAATT GCTCAAGTCG TTAAAGAGTT CTCCCGTCTT 
CCATACGCAA AAATTCTGAA ACACACAATT CTCGCAAGCA GAGAACAATC TTGCATCAAT 
CCGGCCGCTC GTAAACACGC AGACATCTCA CAATACTGTA AAGAAGTGAA CAGTGCACAT 
TCGATTGGTT GTTCATTCAA AAGTGCTATG AAACCACGAT TTGAGAAAGC TTTACCGTTA 
AGGGATCATT TAGAGCGAAA TGGAACAGTG GTATTCGATA TGGAAAAAAT TGTCGAAACG 
TTGGCCATAT CATATCCTCA GCTGTGTCCC TACTTCAGCA CAAACCGAAT TCTCACCCAA 
GACGCTGACA TCATTTTCTG TCCATTTTCG TATCTGGTGG ACCCATTGAT TCGAAATTCG 
TCAGATGTTC AGATAAGAAA CTCGATTGTC ATTTTGGATG AAGCTCATAA CATCGAAGAC 
ACGTGCAGAG AAGCGGCAAG CTTCTCGTTC ACCGAGAAAG AGCTCGATGA TTCGATATTG 
TCGCTGAGAA TTAAAAGAAA AGCAGTTGAC ACGGAATTGG ATAAAACCGG CATCGAAGAG 
AAGCTTCTCC ACATTCCAGA TCGAGAAGTG CATCTAAAAG AATTCCGTGG ACATCTTCTC 
TACCTTGAAC ACCTGATCCT TGAGATGCTT CGATGGATCC GACAAGTGTC GACGGAGGCG 
AAAACGCCGG CTAGAGGTGG TCAGGATGGT GTGAAGACAT GCACAATATC GTCTGCAAAT 
CTTTTTACAT CATTGACCGA TCCAGAAAAT GGTGTCGATC TGTTCACACC ACCACTGACA 
CCCAAACATG CAGCAGTGGC GGCGTCTTTC AGTGCGATTA CGAAGCATAA TGAGCCTGAA 
ATGCAGTACT TTGACGCGTT CAAGCCAAGC TCTACAGCGA TAGTCTGCAT AGAGAAATGG 
TTGTATTTTC AGTCATATTT TGGAAATCAG CAGTACCAAT CCACGTATAG GTTAAACATC 
TCTATTGAGC CGATAAATCA AAATGGGCGA TTCAATCATA CTTTTGACGC AGACGTCTCG 
ATGTCGACTT CATTTGGAAA TCCAAGACCA ACAACGACAA GAAGCTCGGC GGGACCCAGA 
AACATGCAGT ACAAGGAGGA GAACGCATGG CTAGCAGATG CTGCAGCTGA CGGAGACGAT 
TGGAAGGATC CGTCAATGTC AGAGACGGGT CATAAACCGA TTTCAGAGGG CTGCAAGACT 
ACGATCAGCT TGTGGTGTAT GAGTCCAGCA TTGTCTTTCT TCGACGCATT CAACGAAACA 
CGTTCTATAG TCCTCGCATC GGGTACTCTG TGCCCAATGG ACACTTTGAA AACCGAATTA 
GGAATGGAAT TCAAGCAACA AGTAGAAGGA GATCAAGTCA TTAATAAGGA TAACATTTTC 
GCAGCGGTTT TGCCAATTGG TCCCTTTGGA AATCGCATTC AGTGCACGTA CCGCAACACG 
TCAGATCCGG AATCTTCATT TTATTGCGAG CTTGGAGCAA TTATCAAGTA TGTATGCAGT 
AACGTGCCAG CAGGAATTCT ATGTTTTCTC CCCAGCTATC GAGTTCTAGA TCAATTAAAA 
CAATGTATGA TCCGTAATTC AACAATGCGA CAAATCGAAA TGAAAAAAGT TGTGCTCTAC 
GAGCCGCGAA GATCCTCAGA ATTAACATCA GTAATGGATC AATTCGATGC GGCGATCTTC 
GATCCGTCTA GATTCGGTGC AAACATCAAC GGATCTCTTA TGTTTGCCGT TTTTCGTGGA 
AAAGTTTCCG AGGGCATTGA TTTTGCCGAT GATCGTGCGC GTGTGGTGAT TTCTGTCGGA 
ATTCCGTATC CAAATGCGAT GGATGATCAA GTGAATGCGA AAAAGTTGTA TAATGATCAG 
AATTCCAAGG AAAAGGGGAT TTTGACTGGC GATGAGTGGT ATACCACGCA GGCCTATCGA 
GCATTGAATC AGGCTTTGGG AAGATGTCTC CGGCACAAAA ACGACTGGGG AGCAATGCTA 
ATGATCGATG AACGCCTAGA ACGTCAAACT GGAAATCTTG TGGGCGGAGC CTCGTCTGCT 
CGTGTCTCAA AATGGATTCG TGCTCAACTG AAAAGCTATC CGAGTTTTAA AGAATTTAAT 
GCGAATTTTC GCGAATTCAT CCAGCGTCGA CACGCTGTTG AGAAAGCAAA AAAAGAGAAT 
TTTTGTGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dog-1 ( NM_061217.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_493618.1 Caenorhabditis elegans Helicase ATP-binding domain-containing protein (dog-1), mRNA.

Price & Availability↑

CloneID	OCa335774
Clone ID Related Accession (Same CDS sequence)	NM_061217.5 , NM_061217.6
Accession Version	NM_061217.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Helicase ATP-binding domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_061217.5.

ATGTCCTCAA GCGATGCATT TTGGAGAATG TTCGCAAACA AAAACAAAGG AAAATCGAAT 
ACCCGATCGG CATTTCAAGT TGTGAAAGAG GAGCAGCCGT CCACATCAAC AGAACCCGAT 
GATAAAAAGC CGTTACACCA TGAAATTGCC GGGGAGATGA TTAAAAATCC GGCAAAAGGA 
AAACGAAAGC GGGTTCAGAT CAAAAACGAC GAGTATGAGC AGCTCATGAT GCTCGGTGTA 
CCGGTACGCG TGCCACGTGG ATTGAGCCTC TACTCGACTC AAAAACTAAT GATCGTGAGA 
ATTCTGACGG CGCTGAAAAA TAGTCAAAAT GTGCTTGGCG AGTCGCCGAC GGGAAGTGGC 
AAAACTATGG CTCTGCTGGC ATCTACGTGT GCATGGCTCA AGCAGTATAT CGATGAGAAG 
CGAGAATCAA AGGAAAAATG TGAAATCCAT GGATTTTCAG GAAAAACACA GGTGGTGTTC 
ACTAACGATA TTGGAATACC GCTAGAATCT AAAGTATATG AAGAGCCACC GGAAGAAGAG 
GAATTCATTG AGCCTGTGCC AGTGAAAAAC GATAAACTTT TCGAGGCACA ATGGGAGCAC 
GATTTCACGC CTCATACACC AGGAGAAACA CCGCCCGTCA CATTGAAAGA AGAGCTGGAG 
CCGGTAAAAA AACCGGAGCC CGTTGAGACG AAATGCACGT GTCTTCCTCG CGTCCGAATC 
TACTACGGCA CCCGAACCCA CAAGCAAATT GCTCAAGTCG TTAAAGAGTT CTCCCGTCTT 
CCATACGCAA AAATTCTGAA ACACACAATT CTCGCAAGCA GAGAACAATC TTGCATCAAT 
CCGGCCGCTC GTAAACACGC AGACATCTCA CAATACTGTA AAGAAGTGAA CAGTGCACAT 
TCGATTGGTT GTTCATTCAA AAGTGCTATG AAACCACGAT TTGAGAAAGC TTTACCGTTA 
AGGGATCATT TAGAGCGAAA TGGAACAGTG GTATTCGATA TGGAAAAAAT TGTCGAAACG 
TTGGCCATAT CATATCCTCA GCTGTGTCCC TACTTCAGCA CAAACCGAAT TCTCACCCAA 
GACGCTGACA TCATTTTCTG TCCATTTTCG TATCTGGTGG ACCCATTGAT TCGAAATTCG 
TCAGATGTTC AGATAAGAAA CTCGATTGTC ATTTTGGATG AAGCTCATAA CATCGAAGAC 
ACGTGCAGAG AAGCGGCAAG CTTCTCGTTC ACCGAGAAAG AGCTCGATGA TTCGATATTG 
TCGCTGAGAA TTAAAAGAAA AGCAGTTGAC ACGGAATTGG ATAAAACCGG CATCGAAGAG 
AAGCTTCTCC ACATTCCAGA TCGAGAAGTG CATCTAAAAG AATTCCGTGG ACATCTTCTC 
TACCTTGAAC ACCTGATCCT TGAGATGCTT CGATGGATCC GACAAGTGTC GACGGAGGCG 
AAAACGCCGG CTAGAGGTGG TCAGGATGGT GTGAAGACAT GCACAATATC GTCTGCAAAT 
CTTTTTACAT CATTGACCGA TCCAGAAAAT GGTGTCGATC TGTTCACACC ACCACTGACA 
CCCAAACATG CAGCAGTGGC GGCGTCTTTC AGTGCGATTA CGAAGCATAA TGAGCCTGAA 
ATGCAGTACT TTGACGCGTT CAAGCCAAGC TCTACAGCGA TAGTCTGCAT AGAGAAATGG 
TTGTATTTTC AGTCATATTT TGGAAATCAG CAGTACCAAT CCACGTATAG GTTAAACATC 
TCTATTGAGC CGATAAATCA AAATGGGCGA TTCAATCATA CTTTTGACGC AGACGTCTCG 
ATGTCGACTT CATTTGGAAA TCCAAGACCA ACAACGACAA GAAGCTCGGC GGGACCCAGA 
AACATGCAGT ACAAGGAGGA GAACGCATGG CTAGCAGATG CTGCAGCTGA CGGAGACGAT 
TGGAAGGATC CGTCAATGTC AGAGACGGGT CATAAACCGA TTTCAGAGGG CTGCAAGACT 
ACGATCAGCT TGTGGTGTAT GAGTCCAGCA TTGTCTTTCT TCGACGCATT CAACGAAACA 
CGTTCTATAG TCCTCGCATC GGGTACTCTG TGCCCAATGG ACACTTTGAA AACCGAATTA 
GGAATGGAAT TCAAGCAACA AGTAGAAGGA GATCAAGTCA TTAATAAGGA TAACATTTTC 
GCAGCGGTTT TGCCAATTGG TCCCTTTGGA AATCGCATTC AGTGCACGTA CCGCAACACG 
TCAGATCCGG AATCTTCATT TTATTGCGAG CTTGGAGCAA TTATCAAGTA TGTATGCAGT 
AACGTGCCAG CAGGAATTCT ATGTTTTCTC CCCAGCTATC GAGTTCTAGA TCAATTAAAA 
CAATGTATGA TCCGTAATTC AACAATGCGA CAAATCGAAA TGAAAAAAGT TGTGCTCTAC 
GAGCCGCGAA GATCCTCAGA ATTAACATCA GTAATGGATC AATTCGATGC GGCGATCTTC 
GATCCGTCTA GATTCGGTGC AAACATCAAC GGATCTCTTA TGTTTGCCGT TTTTCGTGGA 
AAAGTTTCCG AGGGCATTGA TTTTGCCGAT GATCGTGCGC GTGTGGTGAT TTCTGTCGGA 
ATTCCGTATC CAAATGCGAT GGATGATCAA GTGAATGCGA AAAAGTTGTA TAATGATCAG 
AATTCCAAGG AAAAGGGGAT TTTGACTGGC GATGAGTGGT ATACCACGCA GGCCTATCGA 
GCATTGAATC AGGCTTTGGG AAGATGTCTC CGGCACAAAA ACGACTGGGG AGCAATGCTA 
ATGATCGATG AACGCCTAGA ACGTCAAACT GGAAATCTTG TGGGCGGAGC CTCGTCTGCT 
CGTGTCTCAA AATGGATTCG TGCTCAACTG AAAAGCTATC CGAGTTTTAA AGAATTTAAT 
GCGAATTTTC GCGAATTCAT CCAGCGTCGA CACGCTGTTG AGAAAGCAAA AAAAGAGAAT 
TTTTGTGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_493618.1
CDS	38..2989
Translation	MSSSDAFWRMFANKNKGKSNTRSAFQVVKEEQPSTSTEPDDKKPLHHEIAGEMIKNPAKGKRKRVQIKNDEYEQLMMLGVPVRVPRGLSLYSTQKLMIVRILTALKNSQNVLGESPTGSGKTMALLASTCAWLKQYIDEKRESKEKCEIHGFSGKTQVVFTNDIGIPLESKVYEEPPEEEEFIEPVPVKNDKLFEAQWEHDFTPHTPGETPPVTLKEELEPVKKPEPVETKCTCLPRVRIYYGTRTHKQIAQVVKEFSRLPYAKILKHTILASREQSCINPAARKHADISQYCKEVNSAHSIGCSFKSAMKPRFEKALPLRDHLERNGTVVFDMEKIVETLAISYPQLCPYFSTNRILTQDADIIFCPFSYLVDPLIRNSSDVQIRNSIVILDEAHNIEDTCREAASFSFTEKELDDSILSLRIKRKAVDTELDKTGIEEKLLHIPDREVHLKEFRGHLLYLEHLILEMLRWIRQVSTEAKTPARGGQDGVKTCTISSANLFTSLTDPENGVDLFTPPLTPKHAAVAASFSAITKHNEPEMQYFDAFKPSSTAIVCIEKWLYFQSYFGNQQYQSTYRLNISIEPINQNGRFNHTFDADVSMSTSFGNPRPTTTRSSAGPRNMQYKEENAWLADAAADGDDWKDPSMSETGHKPISEGCKTTISLWCMSPALSFFDAFNETRSIVLASGTLCPMDTLKTELGMEFKQQVEGDQVINKDNIFAAVLPIGPFGNRIQCTYRNTSDPESSFYCELGAIIKYVCSNVPAGILCFLPSYRVLDQLKQCMIRNSTMRQIEMKKVVLYEPRRSSELTSVMDQFDAAIFDPSRFGANINGSLMFAVFRGKVSEGIDFADDRARVVISVGIPYPNAMDDQVNAKKLYNDQNSKEKGILTGDEWYTTQAYRALNQALGRCLRHKNDWGAMLMIDERLERQTGNLVGGASSARVSKWIRAQLKSYPSFKEFNANFREFIQRRHAVEKAKKENFCE

Target ORF information:

RefSeq Version	NM_061217.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Helicase ATP-binding domain-containing protein (dog-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_061217.6

ORF Insert Sequence:

ATGTCCTCAA GCGATGCATT TTGGAGAATG TTCGCAAACA AAAACAAAGG AAAATCGAAT 
ACCCGATCGG CATTTCAAGT TGTGAAAGAG GAGCAGCCGT CCACATCAAC AGAACCCGAT 
GATAAAAAGC CGTTACACCA TGAAATTGCC GGGGAGATGA TTAAAAATCC GGCAAAAGGA 
AAACGAAAGC GGGTTCAGAT CAAAAACGAC GAGTATGAGC AGCTCATGAT GCTCGGTGTA 
CCGGTACGCG TGCCACGTGG ATTGAGCCTC TACTCGACTC AAAAACTAAT GATCGTGAGA 
ATTCTGACGG CGCTGAAAAA TAGTCAAAAT GTGCTTGGCG AGTCGCCGAC GGGAAGTGGC 
AAAACTATGG CTCTGCTGGC ATCTACGTGT GCATGGCTCA AGCAGTATAT CGATGAGAAG 
CGAGAATCAA AGGAAAAATG TGAAATCCAT GGATTTTCAG GAAAAACACA GGTGGTGTTC 
ACTAACGATA TTGGAATACC GCTAGAATCT AAAGTATATG AAGAGCCACC GGAAGAAGAG 
GAATTCATTG AGCCTGTGCC AGTGAAAAAC GATAAACTTT TCGAGGCACA ATGGGAGCAC 
GATTTCACGC CTCATACACC AGGAGAAACA CCGCCCGTCA CATTGAAAGA AGAGCTGGAG 
CCGGTAAAAA AACCGGAGCC CGTTGAGACG AAATGCACGT GTCTTCCTCG CGTCCGAATC 
TACTACGGCA CCCGAACCCA CAAGCAAATT GCTCAAGTCG TTAAAGAGTT CTCCCGTCTT 
CCATACGCAA AAATTCTGAA ACACACAATT CTCGCAAGCA GAGAACAATC TTGCATCAAT 
CCGGCCGCTC GTAAACACGC AGACATCTCA CAATACTGTA AAGAAGTGAA CAGTGCACAT 
TCGATTGGTT GTTCATTCAA AAGTGCTATG AAACCACGAT TTGAGAAAGC TTTACCGTTA 
AGGGATCATT TAGAGCGAAA TGGAACAGTG GTATTCGATA TGGAAAAAAT TGTCGAAACG 
TTGGCCATAT CATATCCTCA GCTGTGTCCC TACTTCAGCA CAAACCGAAT TCTCACCCAA 
GACGCTGACA TCATTTTCTG TCCATTTTCG TATCTGGTGG ACCCATTGAT TCGAAATTCG 
TCAGATGTTC AGATAAGAAA CTCGATTGTC ATTTTGGATG AAGCTCATAA CATCGAAGAC 
ACGTGCAGAG AAGCGGCAAG CTTCTCGTTC ACCGAGAAAG AGCTCGATGA TTCGATATTG 
TCGCTGAGAA TTAAAAGAAA AGCAGTTGAC ACGGAATTGG ATAAAACCGG CATCGAAGAG 
AAGCTTCTCC ACATTCCAGA TCGAGAAGTG CATCTAAAAG AATTCCGTGG ACATCTTCTC 
TACCTTGAAC ACCTGATCCT TGAGATGCTT CGATGGATCC GACAAGTGTC GACGGAGGCG 
AAAACGCCGG CTAGAGGTGG TCAGGATGGT GTGAAGACAT GCACAATATC GTCTGCAAAT 
CTTTTTACAT CATTGACCGA TCCAGAAAAT GGTGTCGATC TGTTCACACC ACCACTGACA 
CCCAAACATG CAGCAGTGGC GGCGTCTTTC AGTGCGATTA CGAAGCATAA TGAGCCTGAA 
ATGCAGTACT TTGACGCGTT CAAGCCAAGC TCTACAGCGA TAGTCTGCAT AGAGAAATGG 
TTGTATTTTC AGTCATATTT TGGAAATCAG CAGTACCAAT CCACGTATAG GTTAAACATC 
TCTATTGAGC CGATAAATCA AAATGGGCGA TTCAATCATA CTTTTGACGC AGACGTCTCG 
ATGTCGACTT CATTTGGAAA TCCAAGACCA ACAACGACAA GAAGCTCGGC GGGACCCAGA 
AACATGCAGT ACAAGGAGGA GAACGCATGG CTAGCAGATG CTGCAGCTGA CGGAGACGAT 
TGGAAGGATC CGTCAATGTC AGAGACGGGT CATAAACCGA TTTCAGAGGG CTGCAAGACT 
ACGATCAGCT TGTGGTGTAT GAGTCCAGCA TTGTCTTTCT TCGACGCATT CAACGAAACA 
CGTTCTATAG TCCTCGCATC GGGTACTCTG TGCCCAATGG ACACTTTGAA AACCGAATTA 
GGAATGGAAT TCAAGCAACA AGTAGAAGGA GATCAAGTCA TTAATAAGGA TAACATTTTC 
GCAGCGGTTT TGCCAATTGG TCCCTTTGGA AATCGCATTC AGTGCACGTA CCGCAACACG 
TCAGATCCGG AATCTTCATT TTATTGCGAG CTTGGAGCAA TTATCAAGTA TGTATGCAGT 
AACGTGCCAG CAGGAATTCT ATGTTTTCTC CCCAGCTATC GAGTTCTAGA TCAATTAAAA 
CAATGTATGA TCCGTAATTC AACAATGCGA CAAATCGAAA TGAAAAAAGT TGTGCTCTAC 
GAGCCGCGAA GATCCTCAGA ATTAACATCA GTAATGGATC AATTCGATGC GGCGATCTTC 
GATCCGTCTA GATTCGGTGC AAACATCAAC GGATCTCTTA TGTTTGCCGT TTTTCGTGGA 
AAAGTTTCCG AGGGCATTGA TTTTGCCGAT GATCGTGCGC GTGTGGTGAT TTCTGTCGGA 
ATTCCGTATC CAAATGCGAT GGATGATCAA GTGAATGCGA AAAAGTTGTA TAATGATCAG 
AATTCCAAGG AAAAGGGGAT TTTGACTGGC GATGAGTGGT ATACCACGCA GGCCTATCGA 
GCATTGAATC AGGCTTTGGG AAGATGTCTC CGGCACAAAA ACGACTGGGG AGCAATGCTA 
ATGATCGATG AACGCCTAGA ACGTCAAACT GGAAATCTTG TGGGCGGAGC CTCGTCTGCT 
CGTGTCTCAA AATGGATTCG TGCTCAACTG AAAAGCTATC CGAGTTTTAA AGAATTTAAT 
GCGAATTTTC GCGAATTCAT CCAGCGTCGA CACGCTGTTG AGAAAGCAAA AAAAGAGAAT 
TTTTGTGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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dog-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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