UBP3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	UBP3
Entrez Gene ID	856895
Full Name	mRNA-binding ubiquitin-specific protease UBP3
Synonyms	BLM3
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179041.3	NP_011078.3	mRNA-binding ubiquitin-specific protease UBP3

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	UBP3	NP_011078.3
Eremothecium gossypii	AGOS_AGR139C	NP_986805.2
Kluyveromyces lactis	KLLA0E01013g	XP_453994.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Dietrich FS, Mulligan J, Hennessy K, Yelton MA, Allen E, Araujo R, Aviles E, Berno A, Brennan T, Carpenter J, Chen E, Cherry JM, Chung E, Duncan M, Guzman E, Hartzell G, Hunicke-Smith S, Hyman RW, Kayser A, Komp C, Lashkari D, Lew H, Lin D, Mosedale D, Davis RW
Nature387(6632 Suppl)78-81(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ubp3 is essential for proper proteasome function in euploid cells, and deletion of this deubiquitinase leads to further proteasome-mediated proteotoxicity in aneuploid yeast
Title: The pleiotropic deubiquitinase Ubp3 confers aneuploidy tolerance.

We show that PRO1 genetically interacts with UBP3, which encodes ubiquitin-specific protease, and is required for selective autophagy of ribosomes (ribophagy).
Title: ?-Glutamyl kinase is involved in selective autophagy of ribosomes in Saccharomyces cerevisiae.

Data suggest regulated process for removal of proteasomes upon nitrogen starvation; autophagy involves separate processes for dissociation and nuclear export of core (PRE2) and regulatory (RPN1) proteins; only core protein autophagy depends on UBP3.
Title: Starvation Induces Proteasome Autophagy with Different Pathways for Core and Regulatory Particles.

Ubp3 deubiquitinating protease is required for efficient stress granule assembly in Saccharomyces cerevisiae
Title: The Catalytic Activity of the Ubp3 Deubiquitinating Protease Is Required for Efficient Stress Granule Assembly in Saccharomyces cerevisiae.

Taken together, these results demonstrate that Rck1 promotes S. cerevisiae pseudohyphal growth via the activation of Ubp3 phosphorylation.
Title: Rck1 promotes pseudohyphal growth via the activation of Ubp3 phosphorylation in Saccharomyces cerevisiae.

The following UBP3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBP3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05646	NM_001179041.3 Latest version!	Saccharomyces cerevisiae S288C mRNA-binding ubiquitin-specific protease UBP3 (UBP3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBP3 ( NM_001179041.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011078.3 Saccharomyces cerevisiae S288C mRNA-binding ubiquitin-specific protease UBP3 (UBP3), partial mRNA.

Price & Availability↑

CloneID	OSi05646
Clone ID Related Accession (Same CDS sequence)	NM_001179041.3
Accession Version	NM_001179041.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	mRNA-binding ubiquitin-specific protease UBP3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001137). On Jul 30, 2012 this sequence version replaced NM_001179041.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAACATGC AAGACGCTAA CAAAGAAGAG TCGTACTCGA TGTACCCGAA AACCTCTTCT 
CCACCACCAC CTACGCCAAC CAATATGCAG ATTCCTATTT ATCAAGCGCC TTTGCAGATG 
TACGGCTACA CTCAGGCCCC ATATCTATAC CCCACACAAA TACCTGCCTA TTCGTTTAAT 
ATGGTCAACC AAAACCAGCC AATCTACCAT CAAAGTGGCA GCCCACATCA CTTGCCTCCG 
CAAAACAATA TTAACGGCGG AAGCACTACC AATAACAACA ACATTAACAA GAAGAAGTGG 
CACTCTAATG GCATTACCAA TAACAATGGA AGCAGCGGTA ATCAAGGCGC CAACTCTAGC 
GGTAGCGGCA TGAGCTACAA CAAATCCCAC ACCTACCATC ACAATTACTC TAACAATCAT 
ATCCCCATGA TGGCCTCTCC AAACAGTGGC AGCAATGCGG GCATGAAAAA ACAGACCAAC 
TCTTCCAACG GCAACGGTTC TTCGGCTACT TCACCATCGT ACTCTTCCTA CAACTCTTCT 
TCACAGTATG ATTTATACAA GTTTGATGTC ACTAAATTAA AGAATCTCAA GGAAAATTCA 
TCAAACTTGA TTCAATTGCC ACTGTTCATA AACACTACGG AAGCAGAATT TGCTGCGGCA 
AGTGTCCAAA GGTACGAATT AAACATGAAG GCTTTGAACC TAAACTCTGA AAGCTTAGAG 
AACTCATCTG TAGAAAAGAG CTCTGCCCAT CATCACACAA AAAGCCATAG TATACCAAAG 
CATAATGAGG AAGTAAAGAC AGAAACACAT GGGGAAGAAG AAGATGCTCA TGATAAAAAA 
CCACATGCGA GCAAAGATGC GCACGAGCTT AAAAAGAAAA CTGAAGTAAA GAAAGAGGAT 
GCTAAGCAAG ACCGTAACGA AAAAGTTATA CAGGAACCTC AAGCTACTGT TTTACCTGTA 
GTGGATAAGA AGGAACCAGA GGAATCTGTT GAAGAAAATA CTTCCAAGAC ATCTTCACCT 
TCACCATCTC CTCCAGCAGC AAAATCCTGG TCCGCCATAG CATCAGATGC GATTAAAAGT 
AGACAAGCTA GTAACAAAAC AGTCTCCGGA TCGATGGTCA CTAAAACACC AATTTCTGGT 
ACGACCGCAG GCGTTTCATC AACAAACATG GCTGCGGCGA CTATAGGTAA ATCCAGCTCT 
CCCCTGTTGT CCAAGCAGCC TCAGAAAAAG GATAAAAAAT ACGTTCCACC TTCTACAAAG 
GGTATTGAGC CACTGGGTTC GATTGCGTTA AGAATGTGTT TTGATCCCGA TTTCATTAGT 
TACGTTTTAC GGAATAAAGA TGTTGAAAAC AAAATACCAG TCCATTCCAT TATTCCAAGA 
GGCATAATTA ACAGAGCCAA CATTTGTTTT ATGAGTTCTG TGTTACAAGT GTTACTCTAC 
TGTAAGCCAT TTATTGATGT AATTAACGTT CTCAGTACAC GGAATACCAA TTCAAGAGTC 
GGCACATCAT CCTGTAAATT ATTAGATGCT TGTTTGACTA TGTATAAGCA ATTCGATAAG 
GAAACCTATG AGAAAAAATT CCTAGAGAAT GCTGATGATG CTGAAAAAAC CACGGAAAGT 
GATGCAAAAA AATCATCAAA ATCCAAGAGT TTCCAACACT GCGCCACTGC CGATGCTGTC 
AAACCTGACG AATTTTACAA AACTTTGTCT ACTATACCGA AGTTCAAAGA CTTGCAATGG 
GGCCATCAGG AAGACGCAGA AGAATTTTTG ACCCACTTAT TGGACCAATT ACACGAGGAA 
TTAATTTCTG CAATTGATGG CTTAACCGAT AATGAAATTC AAAATATGCT GCAAAGTATT 
AATGATGAAC AATTGAAAGT TTTCTTTATT AGAAATTTGT CACGTTATGG AAAAGCAGAG 
TTTATCAAAA ATGCTAGTCC TAGACTGAAG GAGTTGATAG AAAAATATGG CGTGATCAAT 
GATGACTCTA CCGAAGAAAA TGGTTGGCAT GAAGTGAGCG GATCTAGCAA AAGAGGCAAG 
AAAACTAAGA CCGCTGCCAA GAGGACTGTC GAGATTGTTC CATCACCAAT CTCCAAACTT 
TTCGGTGGCC AGTTCAGATC TGTGTTAGAT ATACCGAACA ATAAGGAATC TCAATCGATT 
ACACTCGATC CGTTCCAAAC AATTCAATTG GACATTTCAG ATGCTGGTGT GAATGATCTA 
GAAACTGCAT TCAAAAAATT TAGTGAATAC GAATTGCTAC CCTTTAAGTC CTCGTCAGGG 
AATGATGTCG AGGCCAAGAA GCAGACTTTT ATTGATAAAT TGCCGCAAGT TCTTTTAATC 
CAATTCAAAA GATTCTCATT CATAAATAAT GTGAACAAAG ACAACGCAAT GACGAACTAT 
AACGCGTACA ATGGACGTAT TGAGAAGATC AGGAAAAAAA TTAAATATGG TCACGAGTTA 
ATCATACCTG AAGAATCAAT GTCTTCCATA ACATTGAAAA ACAACACCTC AGGGATTGAT 
GATAGAAGAT ATAAGCTAAC CGGAGTTATA TACCATCATG GGGTAAGTTC CGATGGCGGT 
CATTACACAG CGGATGTTTA TCATAGCGAG CACAACAAAT GGTATAGAAT AGATGATGTA 
AATATTACCG AACTAGAGGA CGATGACGTT TTGAAAGGTG GCGAAGAAGC TTCTGATTCG 
AGGACTGCCT ATATTTTAAT GTATCAAAAG AGAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011078.3
CDS	1..2739
Translation	MNMQDANKEESYSMYPKTSSPPPPTPTNMQIPIYQAPLQMYGYTQAPYLYPTQIPAYSFNMVNQNQPIYHQSGSPHHLPPQNNINGGSTTNNNNINKKKWHSNGITNNNGSSGNQGANSSGSGMSYNKSHTYHHNYSNNHIPMMASPNSGSNAGMKKQTNSSNGNGSSATSPSYSSYNSSSQYDLYKFDVTKLKNLKENSSNLIQLPLFINTTEAEFAAASVQRYELNMKALNLNSESLENSSVEKSSAHHHTKSHSIPKHNEEVKTETHGEEEDAHDKKPHASKDAHELKKKTEVKKEDAKQDRNEKVIQEPQATVLPVVDKKEPEESVEENTSKTSSPSPSPPAAKSWSAIASDAIKSRQASNKTVSGSMVTKTPISGTTAGVSSTNMAAATIGKSSSPLLSKQPQKKDKKYVPPSTKGIEPLGSIALRMCFDPDFISYVLRNKDVENKIPVHSIIPRGIINRANICFMSSVLQVLLYCKPFIDVINVLSTRNTNSRVGTSSCKLLDACLTMYKQFDKETYEKKFLENADDAEKTTESDAKKSSKSKSFQHCATADAVKPDEFYKTLSTIPKFKDLQWGHQEDAEEFLTHLLDQLHEELISAIDGLTDNEIQNMLQSINDEQLKVFFIRNLSRYGKAEFIKNASPRLKELIEKYGVINDDSTEENGWHEVSGSSKRGKKTKTAAKRTVEIVPSPISKLFGGQFRSVLDIPNNKESQSITLDPFQTIQLDISDAGVNDLETAFKKFSEYELLPFKSSSGNDVEAKKQTFIDKLPQVLLIQFKRFSFINNVNKDNAMTNYNAYNGRIEKIRKKIKYGHELIIPEESMSSITLKNNTSGIDDRRYKLTGVIYHHGVSSDGGHYTADVYHSEHNKWYRIDDVNITELEDDDVLKGGEEASDSRTAYILMYQKRN

Target ORF information:

RefSeq Version	NM_001179041.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C mRNA-binding ubiquitin-specific protease UBP3 (UBP3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179041.3

ORF Insert Sequence:

ATGAACATGC AAGACGCTAA CAAAGAAGAG TCGTACTCGA TGTACCCGAA AACCTCTTCT 
CCACCACCAC CTACGCCAAC CAATATGCAG ATTCCTATTT ATCAAGCGCC TTTGCAGATG 
TACGGCTACA CTCAGGCCCC ATATCTATAC CCCACACAAA TACCTGCCTA TTCGTTTAAT 
ATGGTCAACC AAAACCAGCC AATCTACCAT CAAAGTGGCA GCCCACATCA CTTGCCTCCG 
CAAAACAATA TTAACGGCGG AAGCACTACC AATAACAACA ACATTAACAA GAAGAAGTGG 
CACTCTAATG GCATTACCAA TAACAATGGA AGCAGCGGTA ATCAAGGCGC CAACTCTAGC 
GGTAGCGGCA TGAGCTACAA CAAATCCCAC ACCTACCATC ACAATTACTC TAACAATCAT 
ATCCCCATGA TGGCCTCTCC AAACAGTGGC AGCAATGCGG GCATGAAAAA ACAGACCAAC 
TCTTCCAACG GCAACGGTTC TTCGGCTACT TCACCATCGT ACTCTTCCTA CAACTCTTCT 
TCACAGTATG ATTTATACAA GTTTGATGTC ACTAAATTAA AGAATCTCAA GGAAAATTCA 
TCAAACTTGA TTCAATTGCC ACTGTTCATA AACACTACGG AAGCAGAATT TGCTGCGGCA 
AGTGTCCAAA GGTACGAATT AAACATGAAG GCTTTGAACC TAAACTCTGA AAGCTTAGAG 
AACTCATCTG TAGAAAAGAG CTCTGCCCAT CATCACACAA AAAGCCATAG TATACCAAAG 
CATAATGAGG AAGTAAAGAC AGAAACACAT GGGGAAGAAG AAGATGCTCA TGATAAAAAA 
CCACATGCGA GCAAAGATGC GCACGAGCTT AAAAAGAAAA CTGAAGTAAA GAAAGAGGAT 
GCTAAGCAAG ACCGTAACGA AAAAGTTATA CAGGAACCTC AAGCTACTGT TTTACCTGTA 
GTGGATAAGA AGGAACCAGA GGAATCTGTT GAAGAAAATA CTTCCAAGAC ATCTTCACCT 
TCACCATCTC CTCCAGCAGC AAAATCCTGG TCCGCCATAG CATCAGATGC GATTAAAAGT 
AGACAAGCTA GTAACAAAAC AGTCTCCGGA TCGATGGTCA CTAAAACACC AATTTCTGGT 
ACGACCGCAG GCGTTTCATC AACAAACATG GCTGCGGCGA CTATAGGTAA ATCCAGCTCT 
CCCCTGTTGT CCAAGCAGCC TCAGAAAAAG GATAAAAAAT ACGTTCCACC TTCTACAAAG 
GGTATTGAGC CACTGGGTTC GATTGCGTTA AGAATGTGTT TTGATCCCGA TTTCATTAGT 
TACGTTTTAC GGAATAAAGA TGTTGAAAAC AAAATACCAG TCCATTCCAT TATTCCAAGA 
GGCATAATTA ACAGAGCCAA CATTTGTTTT ATGAGTTCTG TGTTACAAGT GTTACTCTAC 
TGTAAGCCAT TTATTGATGT AATTAACGTT CTCAGTACAC GGAATACCAA TTCAAGAGTC 
GGCACATCAT CCTGTAAATT ATTAGATGCT TGTTTGACTA TGTATAAGCA ATTCGATAAG 
GAAACCTATG AGAAAAAATT CCTAGAGAAT GCTGATGATG CTGAAAAAAC CACGGAAAGT 
GATGCAAAAA AATCATCAAA ATCCAAGAGT TTCCAACACT GCGCCACTGC CGATGCTGTC 
AAACCTGACG AATTTTACAA AACTTTGTCT ACTATACCGA AGTTCAAAGA CTTGCAATGG 
GGCCATCAGG AAGACGCAGA AGAATTTTTG ACCCACTTAT TGGACCAATT ACACGAGGAA 
TTAATTTCTG CAATTGATGG CTTAACCGAT AATGAAATTC AAAATATGCT GCAAAGTATT 
AATGATGAAC AATTGAAAGT TTTCTTTATT AGAAATTTGT CACGTTATGG AAAAGCAGAG 
TTTATCAAAA ATGCTAGTCC TAGACTGAAG GAGTTGATAG AAAAATATGG CGTGATCAAT 
GATGACTCTA CCGAAGAAAA TGGTTGGCAT GAAGTGAGCG GATCTAGCAA AAGAGGCAAG 
AAAACTAAGA CCGCTGCCAA GAGGACTGTC GAGATTGTTC CATCACCAAT CTCCAAACTT 
TTCGGTGGCC AGTTCAGATC TGTGTTAGAT ATACCGAACA ATAAGGAATC TCAATCGATT 
ACACTCGATC CGTTCCAAAC AATTCAATTG GACATTTCAG ATGCTGGTGT GAATGATCTA 
GAAACTGCAT TCAAAAAATT TAGTGAATAC GAATTGCTAC CCTTTAAGTC CTCGTCAGGG 
AATGATGTCG AGGCCAAGAA GCAGACTTTT ATTGATAAAT TGCCGCAAGT TCTTTTAATC 
CAATTCAAAA GATTCTCATT CATAAATAAT GTGAACAAAG ACAACGCAAT GACGAACTAT 
AACGCGTACA ATGGACGTAT TGAGAAGATC AGGAAAAAAA TTAAATATGG TCACGAGTTA 
ATCATACCTG AAGAATCAAT GTCTTCCATA ACATTGAAAA ACAACACCTC AGGGATTGAT 
GATAGAAGAT ATAAGCTAAC CGGAGTTATA TACCATCATG GGGTAAGTTC CGATGGCGGT 
CATTACACAG CGGATGTTTA TCATAGCGAG CACAACAAAT GGTATAGAAT AGATGATGTA 
AATATTACCG AACTAGAGGA CGATGACGTT TTGAAAGGTG GCGAAGAAGC TTCTGATTCG 
AGGACTGCCT ATATTTTAAT GTATCAAAAG AGAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Nature387(6632 Suppl)78-81(1997 May)
Dietrich FS,Mulligan J,Hennessy K,Yelton MA,Allen E,Araujo R,Aviles E,Berno A,Brennan T,Carpenter J,Chen E,Cherry JM,Chung E,Duncan M,Guzman E,Hartzell G,Hunicke-Smith S,Hyman RW,Kayser A,Komp C,Lashkari D,Lew H,Lin D,Mosedale D,Davis RW

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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UBP3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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