PRP22 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PRP22
Entrez Gene ID	856732
Full Name	DEAH-box ATP-dependent RNA helicase PRP22
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178904.3	NP_010929.3	DEAH-box ATP-dependent RNA helicase PRP22

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DHX8	XP_001154202.1
Canis lupus familiaris (dog)	DHX8	XP_537627.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007212	XP_308573.4
Oryza sativa (rice)	Os06g0343100	NP_001057574.1
Homo sapiens (human)	DHX8	NP_004932.1
Danio rerio (zebrafish)	dhx8	XP_002663993.1
Saccharomyces cerevisiae (baker's yeast)	PRP22	NP_010929.3
Schizosaccharomyces pombe (fission yeast)	prp22	NP_593253.1
Xenopus tropicalis (tropical clawed frog)	LOC733563	XP_002932570.2
Bos taurus (cattle)	DHX8	NP_001193020.1
Mus musculus (house mouse)	Dhx8	NP_659080.2
Magnaporthe oryzae (rice blast fungus)	MGG_08807	XP_003719164.1
	DHX8	XP_002800602.1
Gallus gallus (chicken)	DHX8	XP_418105.3
Kluyveromyces lactis	KLLA0B11638g	XP_452048.1
Neurospora crassa	NCU06318	XP_962580.1
Rattus norvegicus (Norway rat)	Dhx8	NP_001041309.1
Caenorhabditis elegans (roundworm)	mog-5	NP_495019.1
Eremothecium gossypii	AGOS_AAR020W	NP_982561.2
Arabidopsis thaliana (thale cress)	AT3G26560	NP_189288.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Dietrich FS, Mulligan J, Hennessy K, Yelton MA, Allen E, Araujo R, Aviles E, Berno A, Brennan T, Carpenter J, Chen E, Cherry JM, Chung E, Duncan M, Guzman E, Hartzell G, Hunicke-Smith S, Hyman RW, Kayser A, Komp C, Lashkari D, Lew H, Lin D, Mosedale D, Davis RW
Nature387(6632 Suppl)78-81(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

At the catalytic stage of splicing, suboptimal splice sites are repressed by the DEAH-box ATPases Prp16 and Prp22. study shows that these ATPases function further by enabling the spliceosome to search for and utilize alternative branch sites and 3' splice sites.
Title: Spliceosomal DEAH-Box ATPases Remodel Pre-mRNA to Activate Alternative Splice Sites.

NTR complex catalyzes the disassembly of affinity-purified spliceosomes arrested specifically after the ATP-dependent action of DEAH-box Prp2, Prp16, or Prp22 but not at steps before the action of these ATPases or upon their binding to the spliceosome.
Title: Link of NTR-mediated spliceosome disassembly with DEAH-box ATPases Prp2, Prp16, and Prp22.

C-terminal domain of the spliceosomal helicase Prp22
Title: Structural analysis of the C-terminal domain of the spliceosomal helicase Prp22.

Prp22 unwinds duplexes with 3' overhangs; the 3' -> 5' directionality has implications for positioning of Prp22 on the RNA target
Title: Characterization of the NTPase, RNA-binding, and RNA helicase activities of the DEAH-box splicing factor Prp22.

The following PRP22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRP22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05500	NM_001178904.3 Latest version!	Saccharomyces cerevisiae S288C DEAH-box ATP-dependent RNA helicase PRP22 (PRP22), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRP22 ( NM_001178904.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010929.3 Saccharomyces cerevisiae S288C DEAH-box ATP-dependent RNA helicase PRP22 (PRP22), partial mRNA.

Price & Availability↑

CloneID	OSi05500
Clone ID Related Accession (Same CDS sequence)	NM_001178904.3
Accession Version	NM_001178904.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	DEAH-box ATP-dependent RNA helicase PRP22
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001137). On Jul 30, 2012 this sequence version replaced NM_001178904.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCTGATA TATCGAAACT CATAGGAGCT ATTGTGGGAT CTGATGACCC CGTTATCATT 
GAATTTGTGC TGAATATCAT AAATAAATCC GGCAATTTAC AAGAGTTTAT ACGAAATATT 
CAAAAATTGG ATGCCGGTAT ATCCTACGAA GACAGTATTA AGATGTATAA TGCATTTTTA 
GGAAAGCAGG AAGAAGAAAA GGTACGAAAT AAAGTTAAAA GTTCACCGTT AAGTCAAAAA 
ATTAATCAGG TACTGAAAGA TGACGTTAAT TTGGACGACC CTGTTGTGAC TGAATTCGTC 
CTGAGCATTT TGAATAAATC TAAATCAATA ACTGAGTTCC AGGAACAACT GAACTTGATG 
CAAAGCGGAC TCGATAATGA AACAATTTTC AAGATATATC AAATTGCATC ACCACCAGTA 
ATGAAAGAAG AAGTCTCAGT TCTTCCAAGC ACCAAGATAC CAGCAAAGAT AGAAGCAAAG 
ATTGAAGAAG AGGTACAAAA AATAGAAAGT TTAGACCCCA GCCCTGTCCT TCACAAAGTT 
TATGAAGGTA AAGTGAGAAA CATAACTACT TTTGGGTGTT TCGTCCAAAT TTTTGGGACG 
CGAATGAAGA ATTGTGATGG CTTAGTTCAT ATTTCAGAAA TGTCAGACCA AAGAACATTA 
GATCCACATG ATGTGGTGAG ACAAGGCCAA CATATCTTTG TCGAAGTGAT AAAAATACAA 
AATAATGGAA AAATATCGCT CTCCATGAAA AATATTGATC AACATAGTGG AGAAATCCGG 
AAAAGAAATA CTGAAAGTGT CGAAGATAGA GGTAGGTCCA ACGATGCTCA TACAAGCAGA 
AATATGAAAA ACAAAATTAA AAGGCGTGCT TTGACTTCAC CAGAAAGATG GGAAATTCGC 
CAACTAATTG CCAGCGGTGC TGCTTCCATC GATGACTATC CTGAATTGAA GGATGAAATA 
CCCATAAATA CTTCCTATTT GACCGCGAAA AGAGATGACG GCAGTATCGT TAACGGTAAT 
ACAGAGAAAG TGGACTCAAA ATTGGAAGAA CAACAACGAG ACGAGACGGA TGAAATTGAT 
GTCGAGCTGA ATACAGATGA TGGGCCCAAA TTTTTAAAGG ATCAGCAAGT CAAGGGGGCC 
AAGAAATATG AAATGCCTAA AATTACAAAA GTCCCGAGAG GATTTATGAA TCGTTCAGCA 
ATCAACGGCT CTAATGCAAT ACGAGATCAT AGAGAAGAAA AGTTAAGGAA AAAAAGAGAG 
ATTGAACAAC AGATTAGGAA ACAACAGTCG TTTGATGATC CTACGAAAAA CAAAAAAGAT 
TCCAGGAACG AAATTCAAAT GTTGAAAAAC CAGCTGATTG TTACTGAATG GGAAAAAAAT 
AGAATGAATG AATCTATCTC GTATGGCAAA AGAACTTCCT TACCAATCAG TGCACAGCGT 
CAAACTTTGC CTGTATACGC GATGAGGTCT GAATTGATAC AGGCTGTGCG TGACAATCAA 
TTTCTGGTCA TTGTTGGTGA GACAGGTTCG GGTAAAACCA CTCAAATTAC TCAGTATTTA 
GATGAAGAAG GGTTTAGTAA CTATGGGATG ATCGGATGTA CCCAACCTCG TAGGGTTGCC 
GCTGTATCCG TTGCTAAAAG AGTGGCTGAA GAAGTTGGCT GCAAAGTTGG CCATGACGTT 
GGCTATACCA TTAGATTTGA AGATGTTACT GGCCCAGATA CCAGAATAAA ATATATGACT 
GATGGTATGT TACAGAGAGA GGCGTTGTTA GATCCCGAGA TGTCAAAATA TTCAGTTATC 
ATGTTAGATG AAGCTCACGA AAGAACTGTG GCAACTGATG TTTTATTTGC GTTGTTAAAG 
AAAGCGGCTA TTAAAAGGCC AGAATTAAAA GTAATAGTTA CATCGGCTAC TTTGAACTCT 
GCTAAATTTT CTGAGTACTT TTTAAATTGT CCGATTATCA ATATACCTGG TAAAACTTTT 
CCCGTGGAAG TACTCTACTC ACAGACGCCC CAAATGGATT ACATAGAGGC AGCTTTGGAT 
TGTGTAATAG ACATCCATAT TAATGAAGGC CCAGGCGATA TTTTAGTCTT TTTGACTGGA 
CAGGAAGAAA TAGACTCATG CTGTGAAATT TTATATGATA GGGTGAAAAC TTTAGGTGAC 
AGCATCGGCG AATTACTGAT TTTGCCCGTT TATTCTGCTT TGCCAAGTGA AATCCAATCG 
AAAATTTTTG AACCCACTCC AAAGGGCAGT CGAAAAGTCG TATTTGCCAC AAATATTGCG 
GAAACTTCAA TCACTATTGA TGGAATTTAT TATGTCGTGG ACCCTGGATT TGCAAAAATC 
AACATTTATA ATGCAAGAGC AGGTATCGAG CAGTTGATAG TGTCTCCAAT CTCTCAGGCA 
CAGGCAAATC AAAGAAAAGG TAGAGCTGGA AGAACAGGCC CTGGAAAATG CTATCGACTT 
TATACAGAAT CCGCATTTTA CAATGAAATG TTAGAAAACA CGGTTCCAGA AATTCAAAGG 
CAGAATCTTT CTCATACTAT TTTGATGCTA AAGGCCATGG GAATTAATGA TTTATTGAAA 
TTTGACTTCA TGGATCCCCC TCCCAAAAAT TTAATGCTTA ACGCACTGAC AGAATTATAC 
CACCTGCAGT CACTAGATGA CGAGGGAAAA CTAACAAATT TAGGTAAAGA GATGTCCTTA 
TTTCCAATGG ATCCCACTCT ATCACGCTCT TTATTGTCAT CTGTTGATAA TCAGTGCTCG 
GATGAAATTG TTACTATAAT CTCAATGTTG TCAGTACAGA ACGTTTTTTA TCGTCCAAAA 
GATAGGCAAT TGGAAGCAGA TAGCAAGAAG GCAAAGTTTC ATCATCCATA CGGTGACCAT 
TTAACATTAT TGAACGTTTA TACTAGATGG CAGCAGGCAA ATTACTCGGA GCAGTATTGC 
AAAACAAACT TTCTGCATTT TAGACATTTG AAAAGAGCAA GAGATGTTAA AAGCCAAATT 
AGCATGATAT TTAAAAAGAT AGGCTTAAAG TTAATAAGCT GTCATAGTGA TCCTGATTTA 
ATTCGTAAAA CTTTCGTGTC AGGGTTCTTC ATGAACGCTG CTAAGCGAGA CTCACAGGTA 
GGTTATAAAA CCATCAATGG TGGTACAGAG GTTGGAATAC ATCCCTCGAG TTCATTGTAC 
GGAAAAGAGT ATGAATACGT AATGTATCAT AGTATCGTAT TAACTAGCCG GGAATACATG 
TCGCAGGTAA CATCCATTGA GCCCCAATGG CTTCTCGAAG TAGCGCCTCA TTTTTATAAA 
GCTGGAGATG CTGAAAGCCA GTCTAGGAAG AAAGCGAAAA TCATTCCTTT GCATAACAAG 
TTTGCCAAGG ATCAAAATTC ATGGAGACTA AGCTCAATAA GGCAGTCAAG GGAAAGGGCA 
TTAGGTATCA AGAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010929.3
CDS	1..3438
Translation	MSDISKLIGAIVGSDDPVIIEFVLNIINKSGNLQEFIRNIQKLDAGISYEDSIKMYNAFLGKQEEEKVRNKVKSSPLSQKINQVLKDDVNLDDPVVTEFVLSILNKSKSITEFQEQLNLMQSGLDNETIFKIYQIASPPVMKEEVSVLPSTKIPAKIEAKIEEEVQKIESLDPSPVLHKVYEGKVRNITTFGCFVQIFGTRMKNCDGLVHISEMSDQRTLDPHDVVRQGQHIFVEVIKIQNNGKISLSMKNIDQHSGEIRKRNTESVEDRGRSNDAHTSRNMKNKIKRRALTSPERWEIRQLIASGAASIDDYPELKDEIPINTSYLTAKRDDGSIVNGNTEKVDSKLEEQQRDETDEIDVELNTDDGPKFLKDQQVKGAKKYEMPKITKVPRGFMNRSAINGSNAIRDHREEKLRKKREIEQQIRKQQSFDDPTKNKKDSRNEIQMLKNQLIVTEWEKNRMNESISYGKRTSLPISAQRQTLPVYAMRSELIQAVRDNQFLVIVGETGSGKTTQITQYLDEEGFSNYGMIGCTQPRRVAAVSVAKRVAEEVGCKVGHDVGYTIRFEDVTGPDTRIKYMTDGMLQREALLDPEMSKYSVIMLDEAHERTVATDVLFALLKKAAIKRPELKVIVTSATLNSAKFSEYFLNCPIINIPGKTFPVEVLYSQTPQMDYIEAALDCVIDIHINEGPGDILVFLTGQEEIDSCCEILYDRVKTLGDSIGELLILPVYSALPSEIQSKIFEPTPKGSRKVVFATNIAETSITIDGIYYVVDPGFAKINIYNARAGIEQLIVSPISQAQANQRKGRAGRTGPGKCYRLYTESAFYNEMLENTVPEIQRQNLSHTILMLKAMGINDLLKFDFMDPPPKNLMLNALTELYHLQSLDDEGKLTNLGKEMSLFPMDPTLSRSLLSSVDNQCSDEIVTIISMLSVQNVFYRPKDRQLEADSKKAKFHHPYGDHLTLLNVYTRWQQANYSEQYCKTNFLHFRHLKRARDVKSQISMIFKKIGLKLISCHSDPDLIRKTFVSGFFMNAAKRDSQVGYKTINGGTEVGIHPSSSLYGKEYEYVMYHSIVLTSREYMSQVTSIEPQWLLEVAPHFYKAGDAESQSRKKAKIIPLHNKFAKDQNSWRLSSIRQSRERALGIKR

Target ORF information:

RefSeq Version	NM_001178904.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C DEAH-box ATP-dependent RNA helicase PRP22 (PRP22), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178904.3

ORF Insert Sequence:

ATGTCTGATA TATCGAAACT CATAGGAGCT ATTGTGGGAT CTGATGACCC CGTTATCATT 
GAATTTGTGC TGAATATCAT AAATAAATCC GGCAATTTAC AAGAGTTTAT ACGAAATATT 
CAAAAATTGG ATGCCGGTAT ATCCTACGAA GACAGTATTA AGATGTATAA TGCATTTTTA 
GGAAAGCAGG AAGAAGAAAA GGTACGAAAT AAAGTTAAAA GTTCACCGTT AAGTCAAAAA 
ATTAATCAGG TACTGAAAGA TGACGTTAAT TTGGACGACC CTGTTGTGAC TGAATTCGTC 
CTGAGCATTT TGAATAAATC TAAATCAATA ACTGAGTTCC AGGAACAACT GAACTTGATG 
CAAAGCGGAC TCGATAATGA AACAATTTTC AAGATATATC AAATTGCATC ACCACCAGTA 
ATGAAAGAAG AAGTCTCAGT TCTTCCAAGC ACCAAGATAC CAGCAAAGAT AGAAGCAAAG 
ATTGAAGAAG AGGTACAAAA AATAGAAAGT TTAGACCCCA GCCCTGTCCT TCACAAAGTT 
TATGAAGGTA AAGTGAGAAA CATAACTACT TTTGGGTGTT TCGTCCAAAT TTTTGGGACG 
CGAATGAAGA ATTGTGATGG CTTAGTTCAT ATTTCAGAAA TGTCAGACCA AAGAACATTA 
GATCCACATG ATGTGGTGAG ACAAGGCCAA CATATCTTTG TCGAAGTGAT AAAAATACAA 
AATAATGGAA AAATATCGCT CTCCATGAAA AATATTGATC AACATAGTGG AGAAATCCGG 
AAAAGAAATA CTGAAAGTGT CGAAGATAGA GGTAGGTCCA ACGATGCTCA TACAAGCAGA 
AATATGAAAA ACAAAATTAA AAGGCGTGCT TTGACTTCAC CAGAAAGATG GGAAATTCGC 
CAACTAATTG CCAGCGGTGC TGCTTCCATC GATGACTATC CTGAATTGAA GGATGAAATA 
CCCATAAATA CTTCCTATTT GACCGCGAAA AGAGATGACG GCAGTATCGT TAACGGTAAT 
ACAGAGAAAG TGGACTCAAA ATTGGAAGAA CAACAACGAG ACGAGACGGA TGAAATTGAT 
GTCGAGCTGA ATACAGATGA TGGGCCCAAA TTTTTAAAGG ATCAGCAAGT CAAGGGGGCC 
AAGAAATATG AAATGCCTAA AATTACAAAA GTCCCGAGAG GATTTATGAA TCGTTCAGCA 
ATCAACGGCT CTAATGCAAT ACGAGATCAT AGAGAAGAAA AGTTAAGGAA AAAAAGAGAG 
ATTGAACAAC AGATTAGGAA ACAACAGTCG TTTGATGATC CTACGAAAAA CAAAAAAGAT 
TCCAGGAACG AAATTCAAAT GTTGAAAAAC CAGCTGATTG TTACTGAATG GGAAAAAAAT 
AGAATGAATG AATCTATCTC GTATGGCAAA AGAACTTCCT TACCAATCAG TGCACAGCGT 
CAAACTTTGC CTGTATACGC GATGAGGTCT GAATTGATAC AGGCTGTGCG TGACAATCAA 
TTTCTGGTCA TTGTTGGTGA GACAGGTTCG GGTAAAACCA CTCAAATTAC TCAGTATTTA 
GATGAAGAAG GGTTTAGTAA CTATGGGATG ATCGGATGTA CCCAACCTCG TAGGGTTGCC 
GCTGTATCCG TTGCTAAAAG AGTGGCTGAA GAAGTTGGCT GCAAAGTTGG CCATGACGTT 
GGCTATACCA TTAGATTTGA AGATGTTACT GGCCCAGATA CCAGAATAAA ATATATGACT 
GATGGTATGT TACAGAGAGA GGCGTTGTTA GATCCCGAGA TGTCAAAATA TTCAGTTATC 
ATGTTAGATG AAGCTCACGA AAGAACTGTG GCAACTGATG TTTTATTTGC GTTGTTAAAG 
AAAGCGGCTA TTAAAAGGCC AGAATTAAAA GTAATAGTTA CATCGGCTAC TTTGAACTCT 
GCTAAATTTT CTGAGTACTT TTTAAATTGT CCGATTATCA ATATACCTGG TAAAACTTTT 
CCCGTGGAAG TACTCTACTC ACAGACGCCC CAAATGGATT ACATAGAGGC AGCTTTGGAT 
TGTGTAATAG ACATCCATAT TAATGAAGGC CCAGGCGATA TTTTAGTCTT TTTGACTGGA 
CAGGAAGAAA TAGACTCATG CTGTGAAATT TTATATGATA GGGTGAAAAC TTTAGGTGAC 
AGCATCGGCG AATTACTGAT TTTGCCCGTT TATTCTGCTT TGCCAAGTGA AATCCAATCG 
AAAATTTTTG AACCCACTCC AAAGGGCAGT CGAAAAGTCG TATTTGCCAC AAATATTGCG 
GAAACTTCAA TCACTATTGA TGGAATTTAT TATGTCGTGG ACCCTGGATT TGCAAAAATC 
AACATTTATA ATGCAAGAGC AGGTATCGAG CAGTTGATAG TGTCTCCAAT CTCTCAGGCA 
CAGGCAAATC AAAGAAAAGG TAGAGCTGGA AGAACAGGCC CTGGAAAATG CTATCGACTT 
TATACAGAAT CCGCATTTTA CAATGAAATG TTAGAAAACA CGGTTCCAGA AATTCAAAGG 
CAGAATCTTT CTCATACTAT TTTGATGCTA AAGGCCATGG GAATTAATGA TTTATTGAAA 
TTTGACTTCA TGGATCCCCC TCCCAAAAAT TTAATGCTTA ACGCACTGAC AGAATTATAC 
CACCTGCAGT CACTAGATGA CGAGGGAAAA CTAACAAATT TAGGTAAAGA GATGTCCTTA 
TTTCCAATGG ATCCCACTCT ATCACGCTCT TTATTGTCAT CTGTTGATAA TCAGTGCTCG 
GATGAAATTG TTACTATAAT CTCAATGTTG TCAGTACAGA ACGTTTTTTA TCGTCCAAAA 
GATAGGCAAT TGGAAGCAGA TAGCAAGAAG GCAAAGTTTC ATCATCCATA CGGTGACCAT 
TTAACATTAT TGAACGTTTA TACTAGATGG CAGCAGGCAA ATTACTCGGA GCAGTATTGC 
AAAACAAACT TTCTGCATTT TAGACATTTG AAAAGAGCAA GAGATGTTAA AAGCCAAATT 
AGCATGATAT TTAAAAAGAT AGGCTTAAAG TTAATAAGCT GTCATAGTGA TCCTGATTTA 
ATTCGTAAAA CTTTCGTGTC AGGGTTCTTC ATGAACGCTG CTAAGCGAGA CTCACAGGTA 
GGTTATAAAA CCATCAATGG TGGTACAGAG GTTGGAATAC ATCCCTCGAG TTCATTGTAC 
GGAAAAGAGT ATGAATACGT AATGTATCAT AGTATCGTAT TAACTAGCCG GGAATACATG 
TCGCAGGTAA CATCCATTGA GCCCCAATGG CTTCTCGAAG TAGCGCCTCA TTTTTATAAA 
GCTGGAGATG CTGAAAGCCA GTCTAGGAAG AAAGCGAAAA TCATTCCTTT GCATAACAAG 
TTTGCCAAGG ATCAAAATTC ATGGAGACTA AGCTCAATAA GGCAGTCAAG GGAAAGGGCA 
TTAGGTATCA AGAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Nature387(6632 Suppl)78-81(1997 May)
Dietrich FS,Mulligan J,Hennessy K,Yelton MA,Allen E,Araujo R,Aviles E,Berno A,Brennan T,Carpenter J,Chen E,Cherry JM,Chung E,Duncan M,Guzman E,Hartzell G,Hunicke-Smith S,Hyman RW,Kayser A,Komp C,Lashkari D,Lew H,Lin D,Mosedale D,Davis RW

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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PRP22 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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