RPN1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	RPN1
Entrez Gene ID	856422
Full Name	proteasome regulatory particle base subunit RPN1
Synonyms	HRD2,NAS1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179157.1	NP_011892.1	proteasome regulatory particle base subunit RPN1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Psmd2	NP_001026809.1
Magnaporthe oryzae (rice blast fungus)	MGG_07120	XP_003715310.1
Homo sapiens (human)	PSMD2	NP_002799.3
Drosophila melanogaster (fruit fly)	Rpn1	NP_649158.1
Saccharomyces cerevisiae (baker's yeast)	RPN1	NP_011892.1
Schizosaccharomyces pombe (fission yeast)	mts4	NP_596171.1
Arabidopsis thaliana (thale cress)	RPN1B	NP_194576.5
Arabidopsis thaliana (thale cress)	RPN1A	NP_565477.1
	PSMD2	XP_001093407.2
Mus musculus (house mouse)	Psmd2	NP_598862.1
Kluyveromyces lactis	KLLA0E11507g	XP_454469.1
Eremothecium gossypii	AGOS_ACR072C	NP_983474.1
Oryza sativa (rice)	Os02g0146700	NP_001045879.1
Pan troglodytes (chimpanzee)	PSMD2	XP_001147451.2
Gallus gallus (chicken)	PSMD2	NP_001012952.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002481	XP_563695.1
Oryza sativa (rice)	Os09g0326800	NP_001062884.1
Canis lupus familiaris (dog)	PSMD2	XP_535824.3
Bos taurus (cattle)	PSMD2	NP_001094667.1
Danio rerio (zebrafish)	psmd2	NP_956840.1
Caenorhabditis elegans (roundworm)	rpn-1	NP_501064.1
Neurospora crassa	NCU07721	XP_961971.2
Xenopus tropicalis (tropical clawed frog)	psmd2	NP_001015819.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The structures of proteasome substrate receptor complexes with the shuttle factors that deliver ubiquitinated proteins to proteasomes have been solved, namely human Rpn13 complexed with PLIC2 and Saccharomyces cerevisiae Rpn1 with Rad23.
Title: Structures of Rpn1 T1:Rad23 and hRpn13:hPLIC2 Reveal Distinct Binding Mechanisms between Substrate Receptors and Shuttle Factors of the Proteasome.

Data suggest regulated process for removal of proteasomes upon nitrogen starvation; autophagy involves separate processes for dissociation and nuclear export of core (PRE2) and regulatory (RPN1) proteins; only core protein autophagy depends on UBP3.
Title: Starvation Induces Proteasome Autophagy with Different Pathways for Core and Regulatory Particles.

Study found that the five known proteasomal ubiquitin receptors in yeast are collectively nonessential for ubiquitin recognition and identified a sixth receptor, Rpn1. A site (T1:) in the Rpn1 toroid recognized ubiquitin and ubiquitin-like (UBL:) domains of substrate shuttling factors.
Title: Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome.

Rpn1 and Rpn2 coordinate ubiquitin processing factors at proteasome.
Title: Rpn1 and Rpn2 coordinate ubiquitin processing factors at proteasome.

The current study identifies residues in the LRR1 domain of Rpn1 that contribute to shuttle receptor docking. It validates Ddi1 as a proteasomal shuttle receptor whose stable binding to the proteasome depends on Rpn1 residue D517.
Title: Identification of a functional docking site in the Rpn1 LRR domain for the UBA-UBL domain protein Ddi1.

The following RPN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05225	NM_001179157.1 Latest version!	Saccharomyces cerevisiae S288C proteasome regulatory particle base subunit RPN1 (RPN1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPN1 ( NM_001179157.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011892.1 Saccharomyces cerevisiae S288C proteasome regulatory particle base subunit RPN1 (RPN1), partial mRNA.

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CloneID	OSi05225
Clone ID Related Accession (Same CDS sequence)	NM_001179157.1
Accession Version	NM_001179157.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	proteasome regulatory particle base subunit RPN1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGTAGACG AAAGTGATAA GAAACAACAG ACTATTGATG AACAATCGCA GATAAGTCCT 
GAAAAGCAGA CACCAAATAA GAAGGATAAA AAAAAGGAGG AAGAGGAGCA GCTATCCGAA 
GAAGATGCAA AGCTTAAAAC AGATTTAGAG CTATTGGTTG AAAGGCTGAA AGAAGACGAT 
TCATCTTTAT ATGAAGCGTC TTTGAATGCC TTAAAGGAAT CGATCAAAAA TTCTACGAGT 
TCTATGACCG CCGTTCCCAA ACCTTTGAAA TTCCTTCGTC CGACATATCC AGACCTATGT 
TCTATCTACG ACAAATGGAC AGATCCAAAC TTGAAATCCT CTTTAGCGGA TGTCTTGTCC 
ATTTTAGCCA TGACATACTC TGAAAATGGT AAACACGATT CATTGAGATA TAGATTACTC 
TCCGACGTTT CTGATTTTGA AGGGTGGGGA CATGAGTACA TCCGTCATTT AGCTTTGGAG 
ATCGGTGAAG TATATAATGA TCAAGTGGAA AAAGATGCTG AAGATGAAAC ATCATCTGAT 
GGATCTAAAA GCGATGGATC TGCGGCTACT TCCGGGTTCG AATTTTCCAA GGAAGATACT 
CTTCGTCTAT GTTTAGACAT TGTCCCATAC TTTTTGAAAC ACAACGGTGA AGAAGACGCG 
GTAGATTTGC TTTTGGAAAT TGAATCTATT GACAAGCTAC CTCAATTTGT CGATGAGAAT 
ACCTTCCAAA GAGTTTGTCA ATATATGGTT GCATGTGTTC CACTTTTGCC ACCTCCTGAA 
GACGTTGCCT TTTTAAAAAC CGCATATTCT ATTTATCTAT CTCAGAACGA ACTTACAGAT 
GCAATTGCTT TGGCAGTTAG ACTAGGGGAA GAGGATATGA TCAGATCCGT TTTCGATGCT 
ACTAGCGATC CAGTGATGCA TAAACAATTA GCATATATTT TAGCCGCACA AAAGACATCG 
TTTGAGTATG AAGGTGTTCA GGATATAATT GGAAATGGTA AATTGTCTGA ACACTTTTTA 
TATCTTGCTA AAGAATTGAA TCTAACTGGG CCAAAAGTCC CAGAAGACAT TTATAAGAGC 
CATTTGGATA ACTCTAAATC TGTGTTCTCA AGCGCCGGCC TAGATTCTGC TCAACAAAAT 
CTAGCTTCGT CATTTGTCAA CGGGTTCTTA AACTTAGGTT ATTGTAACGA TAAATTAATT 
GTGGATAATG ATAATTGGGT ATATAAAACC AAAGGTGACG GTATGACTTC CGCGGTAGCT 
AGTATTGGAT CTATATACCA GTGGAATCTC GATGGTTTGC AACAACTGGA TAAGTATCTC 
TACGTTGATG AGCCAGAAGT TAAAGCTGGT GCATTATTAG GTATTGGTAT TTCCGCTTCC 
GGTGTGCATG ATGGTGAAGT TGAACCAGCT TTGTTACTTT TGCAAGATTA TGTTACTAAC 
CCAGACACTA AAATTAGTTC AGCAGCTATT CTTGGCCTGG GTATTGCATT TGCTGGTAGC 
AAGAATGACG AAGTGTTGGG TTTGTTATTA CCAATTGCCG CATCCACTGA CTTGCCTATT 
GAAACTGCAG CTATGGCTTC CTTAGCTTTG GCTCATGTTT TCGTTGGTAC TTGTAATGGT 
GACATTACAA CTTCTATCAT GGACAACTTT TTAGAGCGTA CAGCCATCGA GTTGAAGACC 
GACTGGGTAA GGTTTTTGGC ACTTGCTTTA GGTATTCTAT ACATGGGCCA AGGTGAACAG 
GTGGATGATG TTTTAGAAAC TATTAGTGCT ATTGAACATC CAATGACATC AGCTATTGAA 
GTATTAGTTG GTTCATGTGC TTATACTGGT ACGGGTGATG TTTTGTTAAT TCAAGATTTG 
TTACATCGTT TGACCCCTAA AAACGTAAAA GGTGAAGAAG ATGCAGATGA AGAAGAAACT 
GCCGAAGGAC AGACTAATAG CATAAGTGAC TTTTTGGGTG AGCAAGTGAA TGAACCTACC 
AAGAACGAGG AAGCTGAGAT CGAAGTGGAC GAAATGGAGG TTGATGCCGA AGGAGAGGAA 
GTTGAAGTTA AAGCAGAAAT AACAGAGAAG AAGAATGGTG AGAGTTTAGA AGGTGAAGAG 
ATAAAATCCG AAGAAAAGAA GGGAAAATCT TCCGATAAGG ATGCTACTAC GGACGGGAAA 
AATGATGATG AGGAGGAAGA AAAAGAAGCA GGCATAGTCG ATGAACTTGC TTATGCAGTT 
TTGGGTATTG CTTTGATTGC CCTTGGTGAA GATATTGGCA AGGAAATGTC TTTACGTCAT 
TTTGGTCATT TAATGCATTA TGGTAATGAA CACATCCGTC GTATGGTTCC TTTAGCGATG 
GGTATTGTTT CCGTTTCTGA TCCACAAATG AAGGTGTTCG ATACCTTAAC TCGTTTTTCA 
CATGACGCCG ATTTAGAAGT TTCAATGAAT TCAATTTTCG CTATGGGTCT ATGTGGTGCT 
GGTACTAACA ATGCAAGGTT AGCTCAACTA TTAAGACAGT TGGCAAGTTA TTATTCACGT 
GAACAAGACG CTTTGTTTAT CACCAGATTA GCACAAGGGT TATTGCATCT TGGTAAAGGT 
ACTATGACAA TGGATGTGTT CAATGATGCA CATGTTTTGA ATAAGGTGAC ATTAGCATCA 
ATATTAACTA CCGCAGTTGG TTTAGTATCG CCAAGTTTCA TGTTGAAGCA TCATCAATTG 
TTCTACATGT TGAATGCCGG TATAAGGCCA AAGTTTATTC TAGCATTAAA CGATGAGGGA 
GAGCCAATAA AGGTTAATGT GCGCGTTGGT CAAGCAGTGG AAACTGTCGG CCAAGCAGGC 
AGACCAAAAA AGATTACTGG CTGGATTACG CAATCCACTC CTGTTTTACT AAATCATGGC 
GAAAGAGCAG AACTAGAAAC TGATGAATAT ATTAGTTACA CAAGCCATAT TGAGGGCGTA 
GTAATTTTAA AGAAGAACCC TGACTATCGT GAAGAGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011892.1
CDS	1..2982
Translation	MVDESDKKQQTIDEQSQISPEKQTPNKKDKKKEEEEQLSEEDAKLKTDLELLVERLKEDDSSLYEASLNALKESIKNSTSSMTAVPKPLKFLRPTYPDLCSIYDKWTDPNLKSSLADVLSILAMTYSENGKHDSLRYRLLSDVSDFEGWGHEYIRHLALEIGEVYNDQVEKDAEDETSSDGSKSDGSAATSGFEFSKEDTLRLCLDIVPYFLKHNGEEDAVDLLLEIESIDKLPQFVDENTFQRVCQYMVACVPLLPPPEDVAFLKTAYSIYLSQNELTDAIALAVRLGEEDMIRSVFDATSDPVMHKQLAYILAAQKTSFEYEGVQDIIGNGKLSEHFLYLAKELNLTGPKVPEDIYKSHLDNSKSVFSSAGLDSAQQNLASSFVNGFLNLGYCNDKLIVDNDNWVYKTKGDGMTSAVASIGSIYQWNLDGLQQLDKYLYVDEPEVKAGALLGIGISASGVHDGEVEPALLLLQDYVTNPDTKISSAAILGLGIAFAGSKNDEVLGLLLPIAASTDLPIETAAMASLALAHVFVGTCNGDITTSIMDNFLERTAIELKTDWVRFLALALGILYMGQGEQVDDVLETISAIEHPMTSAIEVLVGSCAYTGTGDVLLIQDLLHRLTPKNVKGEEDADEEETAEGQTNSISDFLGEQVNEPTKNEEAEIEVDEMEVDAEGEEVEVKAEITEKKNGESLEGEEIKSEEKKGKSSDKDATTDGKNDDEEEEKEAGIVDELAYAVLGIALIALGEDIGKEMSLRHFGHLMHYGNEHIRRMVPLAMGIVSVSDPQMKVFDTLTRFSHDADLEVSMNSIFAMGLCGAGTNNARLAQLLRQLASYYSREQDALFITRLAQGLLHLGKGTMTMDVFNDAHVLNKVTLASILTTAVGLVSPSFMLKHHQLFYMLNAGIRPKFILALNDEGEPIKVNVRVGQAVETVGQAGRPKKITGWITQSTPVLLNHGERAELETDEYISYTSHIEGVVILKKNPDYREEE

Target ORF information:

RefSeq Version	NM_001179157.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C proteasome regulatory particle base subunit RPN1 (RPN1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179157.1

ORF Insert Sequence:

ATGGTAGACG AAAGTGATAA GAAACAACAG ACTATTGATG AACAATCGCA GATAAGTCCT 
GAAAAGCAGA CACCAAATAA GAAGGATAAA AAAAAGGAGG AAGAGGAGCA GCTATCCGAA 
GAAGATGCAA AGCTTAAAAC AGATTTAGAG CTATTGGTTG AAAGGCTGAA AGAAGACGAT 
TCATCTTTAT ATGAAGCGTC TTTGAATGCC TTAAAGGAAT CGATCAAAAA TTCTACGAGT 
TCTATGACCG CCGTTCCCAA ACCTTTGAAA TTCCTTCGTC CGACATATCC AGACCTATGT 
TCTATCTACG ACAAATGGAC AGATCCAAAC TTGAAATCCT CTTTAGCGGA TGTCTTGTCC 
ATTTTAGCCA TGACATACTC TGAAAATGGT AAACACGATT CATTGAGATA TAGATTACTC 
TCCGACGTTT CTGATTTTGA AGGGTGGGGA CATGAGTACA TCCGTCATTT AGCTTTGGAG 
ATCGGTGAAG TATATAATGA TCAAGTGGAA AAAGATGCTG AAGATGAAAC ATCATCTGAT 
GGATCTAAAA GCGATGGATC TGCGGCTACT TCCGGGTTCG AATTTTCCAA GGAAGATACT 
CTTCGTCTAT GTTTAGACAT TGTCCCATAC TTTTTGAAAC ACAACGGTGA AGAAGACGCG 
GTAGATTTGC TTTTGGAAAT TGAATCTATT GACAAGCTAC CTCAATTTGT CGATGAGAAT 
ACCTTCCAAA GAGTTTGTCA ATATATGGTT GCATGTGTTC CACTTTTGCC ACCTCCTGAA 
GACGTTGCCT TTTTAAAAAC CGCATATTCT ATTTATCTAT CTCAGAACGA ACTTACAGAT 
GCAATTGCTT TGGCAGTTAG ACTAGGGGAA GAGGATATGA TCAGATCCGT TTTCGATGCT 
ACTAGCGATC CAGTGATGCA TAAACAATTA GCATATATTT TAGCCGCACA AAAGACATCG 
TTTGAGTATG AAGGTGTTCA GGATATAATT GGAAATGGTA AATTGTCTGA ACACTTTTTA 
TATCTTGCTA AAGAATTGAA TCTAACTGGG CCAAAAGTCC CAGAAGACAT TTATAAGAGC 
CATTTGGATA ACTCTAAATC TGTGTTCTCA AGCGCCGGCC TAGATTCTGC TCAACAAAAT 
CTAGCTTCGT CATTTGTCAA CGGGTTCTTA AACTTAGGTT ATTGTAACGA TAAATTAATT 
GTGGATAATG ATAATTGGGT ATATAAAACC AAAGGTGACG GTATGACTTC CGCGGTAGCT 
AGTATTGGAT CTATATACCA GTGGAATCTC GATGGTTTGC AACAACTGGA TAAGTATCTC 
TACGTTGATG AGCCAGAAGT TAAAGCTGGT GCATTATTAG GTATTGGTAT TTCCGCTTCC 
GGTGTGCATG ATGGTGAAGT TGAACCAGCT TTGTTACTTT TGCAAGATTA TGTTACTAAC 
CCAGACACTA AAATTAGTTC AGCAGCTATT CTTGGCCTGG GTATTGCATT TGCTGGTAGC 
AAGAATGACG AAGTGTTGGG TTTGTTATTA CCAATTGCCG CATCCACTGA CTTGCCTATT 
GAAACTGCAG CTATGGCTTC CTTAGCTTTG GCTCATGTTT TCGTTGGTAC TTGTAATGGT 
GACATTACAA CTTCTATCAT GGACAACTTT TTAGAGCGTA CAGCCATCGA GTTGAAGACC 
GACTGGGTAA GGTTTTTGGC ACTTGCTTTA GGTATTCTAT ACATGGGCCA AGGTGAACAG 
GTGGATGATG TTTTAGAAAC TATTAGTGCT ATTGAACATC CAATGACATC AGCTATTGAA 
GTATTAGTTG GTTCATGTGC TTATACTGGT ACGGGTGATG TTTTGTTAAT TCAAGATTTG 
TTACATCGTT TGACCCCTAA AAACGTAAAA GGTGAAGAAG ATGCAGATGA AGAAGAAACT 
GCCGAAGGAC AGACTAATAG CATAAGTGAC TTTTTGGGTG AGCAAGTGAA TGAACCTACC 
AAGAACGAGG AAGCTGAGAT CGAAGTGGAC GAAATGGAGG TTGATGCCGA AGGAGAGGAA 
GTTGAAGTTA AAGCAGAAAT AACAGAGAAG AAGAATGGTG AGAGTTTAGA AGGTGAAGAG 
ATAAAATCCG AAGAAAAGAA GGGAAAATCT TCCGATAAGG ATGCTACTAC GGACGGGAAA 
AATGATGATG AGGAGGAAGA AAAAGAAGCA GGCATAGTCG ATGAACTTGC TTATGCAGTT 
TTGGGTATTG CTTTGATTGC CCTTGGTGAA GATATTGGCA AGGAAATGTC TTTACGTCAT 
TTTGGTCATT TAATGCATTA TGGTAATGAA CACATCCGTC GTATGGTTCC TTTAGCGATG 
GGTATTGTTT CCGTTTCTGA TCCACAAATG AAGGTGTTCG ATACCTTAAC TCGTTTTTCA 
CATGACGCCG ATTTAGAAGT TTCAATGAAT TCAATTTTCG CTATGGGTCT ATGTGGTGCT 
GGTACTAACA ATGCAAGGTT AGCTCAACTA TTAAGACAGT TGGCAAGTTA TTATTCACGT 
GAACAAGACG CTTTGTTTAT CACCAGATTA GCACAAGGGT TATTGCATCT TGGTAAAGGT 
ACTATGACAA TGGATGTGTT CAATGATGCA CATGTTTTGA ATAAGGTGAC ATTAGCATCA 
ATATTAACTA CCGCAGTTGG TTTAGTATCG CCAAGTTTCA TGTTGAAGCA TCATCAATTG 
TTCTACATGT TGAATGCCGG TATAAGGCCA AAGTTTATTC TAGCATTAAA CGATGAGGGA 
GAGCCAATAA AGGTTAATGT GCGCGTTGGT CAAGCAGTGG AAACTGTCGG CCAAGCAGGC 
AGACCAAAAA AGATTACTGG CTGGATTACG CAATCCACTC CTGTTTTACT AAATCATGGC 
GAAAGAGCAG AACTAGAAAC TGATGAATAT ATTAGTTACA CAAGCCATAT TGAGGGCGTA 
GTAATTTTAA AGAAGAACCC TGACTATCGT GAAGAGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
Johnston M,Andrews S,Brinkman R,Cooper J,Ding H,Dover J,Du Z,Favello A,Fulton L,Gattung S

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RPN1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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