MPH1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MPH1
Entrez Gene ID	854818
Full Name	3'-5' DNA helicase
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179524.1	NP_012267.1	3'-5' DNA helicase

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0D13552g	XP_453667.2
Saccharomyces cerevisiae (baker's yeast)	MPH1	NP_012267.1
Eremothecium gossypii	AGOS_ADR011C	NP_984107.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
Churcher C, Bowman S, Badcock K, Bankier A, Brown D, Chillingworth T, Connor R, Devlin K, Gentles S, Hamlin N, Harris D, Horsnell T, Hunt S, Jagels K, Jones M, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Rowley N, Skelton J, Smith V, Barrell B
Nature387(6632 Suppl)84-7(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutator phenotype of MPH1 overexpression suggests a novel gain-of-function mechanism. DEAH-box function was required for hypermutation, mutagenesis by MPH1 overexpression was independent of translesion synthesis, but was suppressed by overexpression of RAD27.
Title: Dosage Mutator Genes in Saccharomyces cerevisiae: A Novel Mutator Mode-of-Action of the Mph1 DNA Helicase.

mms21RINGDelta mutant exhibits (1) reduced ribosomal RNA production; (2) nuclear accumulation of ribosomal proteins; (3) elevated Gcn4 translation, indicating translational stress; We identified RPL19A as a novel genetic suppressor of the mms21RINGDelta mutant; Deletion of a previously identified strong suppressor, MPH1, rescues both the accumulation of ribosome subunits and translational stress
Title: Mms21 SUMO Ligase Activity Promotes Nucleolar Function in Saccharomyces cerevisiae.

Mph1regulates the REC-mediated choice between gene conversion and break-induced replication (BIR). Double deletion of Sgs1 and Mph1 abolishes the REC-mediated lag observed during the repair of large gaps and BIR substrates.
Title: Sgs1 and Mph1 Helicases Enforce the Recombination Execution Checkpoint During DNA Double-Strand Break Repair in Saccharomyces cerevisiae.

Mph1 and Mte1 interdependently colocalize at DNA damage-induced foci and dysfunctional telomeres, and MTE1 deletion results in elongated telomeres
Title: Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance.

Mte1 functions with Mph1 and the associated MHF complex in replication fork repair
Title: Differential regulation of the anti-crossover and replication fork regression activities of Mph1 by Mte1.

The following MPH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MPH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03833	NM_001179524.1 Latest version!	Saccharomyces cerevisiae S288C 3'-5' DNA helicase (MPH1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MPH1 ( NM_001179524.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012267.1 Saccharomyces cerevisiae S288C 3'-5' DNA helicase (MPH1), partial mRNA.

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CloneID	OSi03833
Clone ID Related Accession (Same CDS sequence)	NM_001179524.1
Accession Version	NM_001179524.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	3'-5' DNA helicase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001141). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGCTAGTG CAGATGATTA CTTTAGTGAT TTCGAAGATG ACGAGCTGGA TAAACTGTAT 
GAAAAGGCTA TCAATAAATC TGTGAAGGAA ACTATAACGA GACGTGCTGT ACCAGTTCAA 
AAAGACCTTC ATGATAACGT TTTACCTGGG CAGAAAACTG TGTACGAAGA AATTCAAAGA 
GATGTCAGTT TTGGACCCAC TCATCATGAG CTTGATTACG ATGCTTTGTC GTTCTACGTG 
TACCCTACAA ATTACGAGGT AAGGGATTAT CAGTATACAA TTGTTCACAA ATCACTGTTT 
CAAAATACGT TATGCGCCAT CCCAACGGGT ATGGGTAAAA CGTTCATTGC CAGTACAGTA 
ATGTTAAATT ATTTTCGTTG GACCAAAAAA GCCAAAATAA TCTTTACTGC TCCAACAAGA 
CCTTTGGTAG CCCAGCAAAT TAAGGCATGC TTAGGTATAA CAGGAATACC TTCGGATCAA 
ACTGCTATAT TACTCGATAA AAGCAGGAAA AATAGAGAAG AAATTTGGGC CAATAAGAGA 
GTTTTCTTTG CCACACCGCA AGTAGTGGAG AATGACTTGA AGAGGGGTGT ATTGGATCCT 
AAGGATATTG TATGTCTTGT CATCGATGAA GCTCACAGAG CTACTGGCTC CTCTGCGTAT 
ACAAATGTGG TAAAATTCAT AGATAGGTTC AATTCATCAT ACAGATTATT AGCATTGACC 
GCTACACCAG CCTCTGACCT GGAAGGTGTC CAAGAAGTGG TTAATAACTT AGATATTTCC 
AAGATTGAAA TTAGGACTGA AGAAAGCATG GATATCGTGA AATATATGAA GAAAAGAAAA 
AAGGAAAAAA TTGAAGTGCC CCTTTTGTTG GAAATTGAAG ACATAATCGA ACAATTAGGT 
ATGGCCGTCA AACCAGTGCT TCAACAGGCA ATTGAACTCG GTATATATGA GGAATGTGAC 
CCTTCACAAA TAAACGCCTT CAAAGCCATG CAACAAAGCC AAAAAATTAT TGCTAACCCT 
ACTATACCTG AAGGAATTAA GTGGAGGAAC TTCTTTATTT TACAGTTATT GAACAATGTG 
GGTCAAATGC TAAAGAGATT AAAAATTTAT GGAATAAGGA CATTTTTCAA TTACTTCCAG 
AATAAATGTA CAGAATTCAC TACGAAATAT AATCTTAAAA AATCTACTAA TAAAATTGCA 
GCTGAGTTTT ATTACCATCC GATTTTGAAA AACATCAAAA ATCAATGCGA AAATTATTTA 
AGTGATCCAA AGTTTGTCGG TCATGGGAAG TTACAATGTG TTAGAGATGA ACTAATGGAC 
TTTTTTCAGA AAAGGGGTTC CGACTCGAGG GTTATTATTT TTACAGAATT AAGAGAAAGC 
GCACTTGAAA TTGTGAAGTT CATAGACTCG GTAGCTGACG ATCAAATCAG GCCTCATATT 
TTTATTGGAC AAGCTCGTGC AAAAGAAGGT TTTGATGAGG TCAAATACAC AAGAAAACAT 
GCGCCAAAGG GAAGGAAGAA GGTAGAGAGA TTGCATAGGC AAGAGCAAGA GAAATTTTTG 
GAAGCTGAAC GAACTAAGAG AGCGGCGAAT GATAAACTTG AAAGAAGTGC CAGACGAACG 
GGGAGCTCTG AAGAGGCTCA GATTAGCGGA ATGAACCAAA AAATGCAAAA AGAGGTTATC 
CATAATTTCA AAAAGGGTGA ATATAATGTT CTCGTTTGTA CATCAATTGG AGAAGAAGGT 
TTGGATATCG GAGAAGTTGA TTTAATAATC TGTTATGATA CTACAAGTAG TCCAATCAAA 
AATATTCAGC GTATGGGTAG AACAGGCAGA AAACGAGATG GTAAGATAGT ACTACTGTTT 
AGCAGTAATG AATCATACAA ATTCGAGCGT GCTATGGAAG ACTATTCAAC CCTGCAAGCG 
CTTATATCCA AGCAATGCAT TGACTATAAA AAATCAGATC GGATAATCCC TGAAGACATT 
ATTCCGGAAT GTCACGAGAC ATTGATCACG ATTAATGATG AAAACGAAAT AATTAATGAA 
ATGGAAGATG TTGACGAAGT TATTCGTTAT GCGACTCAGT GTATGATGGG CAAAAAGGTG 
AAGCCAAAAA AAGCCATAAC AAAGAAGAAG AGAGTTCAGG AAAATAAAAA ACCGAAGAAG 
TTCTTTATGC CTGATAATGT TGAGACCAGT ATTGTTAGTG CAAGCACTTT AATTAATAAG 
TTCTTGGTGA ATGAAAGTGG CGGGAAACAA CTAGTAACGA GTAATGAAAA CCCAAGTAAG 
AAGCGGAAGA TTTTTAAAGC TTTGGATAAT CTGGAGAATG ATTCAACCGA GGAAGCTTCC 
TCCTCATTAG AAACTGAAGA TGAAGAAGTC AGTGATGATA ATAATGTTTT CATTGCGGAA 
GGGCAGAATG GATGCCAAAA AGATTTGGAA ACTGCCATTA TTCGCACTGG CGAAAGTCTT 
ACCACACTCA AGCCACTTCA TAATTTTGAA CGCCCTAATA TGGCTTTATT TGTGAATGAT 
TGTGGTCTTC CAACTAAAAT CGAGAAAAAT GTGAAAGACA TCCGAGGAAA TCAACACAAC 
CTTGAAAAAG AAAAGAGCTG TACTGTTGAC AAGAATAACA TGGTATTGAG TTTGGACGAT 
TGGAATTTTT TCAGAAACCG CTATATCCCA GAAGGTGTTT CCTTCGATGT GGAGCCCAAT 
TTTGTACAAT ACACCAAAGG GGTAAAAGTT CCGCATTGTC ACAAAGTTTC AAAAATCATT 
ACGTTATTTA ACGACGAGAG TAATGATAAT AAGAAGAGGA CAATTGATAT GAACTATACT 
AAATGTTTGG CGAGAGGCAT GTTACGAGAC GAAAAAAAAT TTGTAAAAGT TAACGATAAA 
AGTCAAGTCG ATAACAACAG TGTCAACCAT GATTCATCCC AATCTTTTAC CTTATCGAAC 
GCAGAATTAG ATGACATACT GGGCTCAGAT TCTGATTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012267.1
CDS	1..2982
Translation	MASADDYFSDFEDDELDKLYEKAINKSVKETITRRAVPVQKDLHDNVLPGQKTVYEEIQRDVSFGPTHHELDYDALSFYVYPTNYEVRDYQYTIVHKSLFQNTLCAIPTGMGKTFIASTVMLNYFRWTKKAKIIFTAPTRPLVAQQIKACLGITGIPSDQTAILLDKSRKNREEIWANKRVFFATPQVVENDLKRGVLDPKDIVCLVIDEAHRATGSSAYTNVVKFIDRFNSSYRLLALTATPASDLEGVQEVVNNLDISKIEIRTEESMDIVKYMKKRKKEKIEVPLLLEIEDIIEQLGMAVKPVLQQAIELGIYEECDPSQINAFKAMQQSQKIIANPTIPEGIKWRNFFILQLLNNVGQMLKRLKIYGIRTFFNYFQNKCTEFTTKYNLKKSTNKIAAEFYYHPILKNIKNQCENYLSDPKFVGHGKLQCVRDELMDFFQKRGSDSRVIIFTELRESALEIVKFIDSVADDQIRPHIFIGQARAKEGFDEVKYTRKHAPKGRKKVERLHRQEQEKFLEAERTKRAANDKLERSARRTGSSEEAQISGMNQKMQKEVIHNFKKGEYNVLVCTSIGEEGLDIGEVDLIICYDTTSSPIKNIQRMGRTGRKRDGKIVLLFSSNESYKFERAMEDYSTLQALISKQCIDYKKSDRIIPEDIIPECHETLITINDENEIINEMEDVDEVIRYATQCMMGKKVKPKKAITKKKRVQENKKPKKFFMPDNVETSIVSASTLINKFLVNESGGKQLVTSNENPSKKRKIFKALDNLENDSTEEASSSLETEDEEVSDDNNVFIAEGQNGCQKDLETAIIRTGESLTTLKPLHNFERPNMALFVNDCGLPTKIEKNVKDIRGNQHNLEKEKSCTVDKNNMVLSLDDWNFFRNRYIPEGVSFDVEPNFVQYTKGVKVPHCHKVSKIITLFNDESNDNKKRTIDMNYTKCLARGMLRDEKKFVKVNDKSQVDNNSVNHDSSQSFTLSNAELDDILGSDSDF

Target ORF information:

RefSeq Version	NM_001179524.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C 3'-5' DNA helicase (MPH1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179524.1

ORF Insert Sequence:

ATGGCTAGTG CAGATGATTA CTTTAGTGAT TTCGAAGATG ACGAGCTGGA TAAACTGTAT 
GAAAAGGCTA TCAATAAATC TGTGAAGGAA ACTATAACGA GACGTGCTGT ACCAGTTCAA 
AAAGACCTTC ATGATAACGT TTTACCTGGG CAGAAAACTG TGTACGAAGA AATTCAAAGA 
GATGTCAGTT TTGGACCCAC TCATCATGAG CTTGATTACG ATGCTTTGTC GTTCTACGTG 
TACCCTACAA ATTACGAGGT AAGGGATTAT CAGTATACAA TTGTTCACAA ATCACTGTTT 
CAAAATACGT TATGCGCCAT CCCAACGGGT ATGGGTAAAA CGTTCATTGC CAGTACAGTA 
ATGTTAAATT ATTTTCGTTG GACCAAAAAA GCCAAAATAA TCTTTACTGC TCCAACAAGA 
CCTTTGGTAG CCCAGCAAAT TAAGGCATGC TTAGGTATAA CAGGAATACC TTCGGATCAA 
ACTGCTATAT TACTCGATAA AAGCAGGAAA AATAGAGAAG AAATTTGGGC CAATAAGAGA 
GTTTTCTTTG CCACACCGCA AGTAGTGGAG AATGACTTGA AGAGGGGTGT ATTGGATCCT 
AAGGATATTG TATGTCTTGT CATCGATGAA GCTCACAGAG CTACTGGCTC CTCTGCGTAT 
ACAAATGTGG TAAAATTCAT AGATAGGTTC AATTCATCAT ACAGATTATT AGCATTGACC 
GCTACACCAG CCTCTGACCT GGAAGGTGTC CAAGAAGTGG TTAATAACTT AGATATTTCC 
AAGATTGAAA TTAGGACTGA AGAAAGCATG GATATCGTGA AATATATGAA GAAAAGAAAA 
AAGGAAAAAA TTGAAGTGCC CCTTTTGTTG GAAATTGAAG ACATAATCGA ACAATTAGGT 
ATGGCCGTCA AACCAGTGCT TCAACAGGCA ATTGAACTCG GTATATATGA GGAATGTGAC 
CCTTCACAAA TAAACGCCTT CAAAGCCATG CAACAAAGCC AAAAAATTAT TGCTAACCCT 
ACTATACCTG AAGGAATTAA GTGGAGGAAC TTCTTTATTT TACAGTTATT GAACAATGTG 
GGTCAAATGC TAAAGAGATT AAAAATTTAT GGAATAAGGA CATTTTTCAA TTACTTCCAG 
AATAAATGTA CAGAATTCAC TACGAAATAT AATCTTAAAA AATCTACTAA TAAAATTGCA 
GCTGAGTTTT ATTACCATCC GATTTTGAAA AACATCAAAA ATCAATGCGA AAATTATTTA 
AGTGATCCAA AGTTTGTCGG TCATGGGAAG TTACAATGTG TTAGAGATGA ACTAATGGAC 
TTTTTTCAGA AAAGGGGTTC CGACTCGAGG GTTATTATTT TTACAGAATT AAGAGAAAGC 
GCACTTGAAA TTGTGAAGTT CATAGACTCG GTAGCTGACG ATCAAATCAG GCCTCATATT 
TTTATTGGAC AAGCTCGTGC AAAAGAAGGT TTTGATGAGG TCAAATACAC AAGAAAACAT 
GCGCCAAAGG GAAGGAAGAA GGTAGAGAGA TTGCATAGGC AAGAGCAAGA GAAATTTTTG 
GAAGCTGAAC GAACTAAGAG AGCGGCGAAT GATAAACTTG AAAGAAGTGC CAGACGAACG 
GGGAGCTCTG AAGAGGCTCA GATTAGCGGA ATGAACCAAA AAATGCAAAA AGAGGTTATC 
CATAATTTCA AAAAGGGTGA ATATAATGTT CTCGTTTGTA CATCAATTGG AGAAGAAGGT 
TTGGATATCG GAGAAGTTGA TTTAATAATC TGTTATGATA CTACAAGTAG TCCAATCAAA 
AATATTCAGC GTATGGGTAG AACAGGCAGA AAACGAGATG GTAAGATAGT ACTACTGTTT 
AGCAGTAATG AATCATACAA ATTCGAGCGT GCTATGGAAG ACTATTCAAC CCTGCAAGCG 
CTTATATCCA AGCAATGCAT TGACTATAAA AAATCAGATC GGATAATCCC TGAAGACATT 
ATTCCGGAAT GTCACGAGAC ATTGATCACG ATTAATGATG AAAACGAAAT AATTAATGAA 
ATGGAAGATG TTGACGAAGT TATTCGTTAT GCGACTCAGT GTATGATGGG CAAAAAGGTG 
AAGCCAAAAA AAGCCATAAC AAAGAAGAAG AGAGTTCAGG AAAATAAAAA ACCGAAGAAG 
TTCTTTATGC CTGATAATGT TGAGACCAGT ATTGTTAGTG CAAGCACTTT AATTAATAAG 
TTCTTGGTGA ATGAAAGTGG CGGGAAACAA CTAGTAACGA GTAATGAAAA CCCAAGTAAG 
AAGCGGAAGA TTTTTAAAGC TTTGGATAAT CTGGAGAATG ATTCAACCGA GGAAGCTTCC 
TCCTCATTAG AAACTGAAGA TGAAGAAGTC AGTGATGATA ATAATGTTTT CATTGCGGAA 
GGGCAGAATG GATGCCAAAA AGATTTGGAA ACTGCCATTA TTCGCACTGG CGAAAGTCTT 
ACCACACTCA AGCCACTTCA TAATTTTGAA CGCCCTAATA TGGCTTTATT TGTGAATGAT 
TGTGGTCTTC CAACTAAAAT CGAGAAAAAT GTGAAAGACA TCCGAGGAAA TCAACACAAC 
CTTGAAAAAG AAAAGAGCTG TACTGTTGAC AAGAATAACA TGGTATTGAG TTTGGACGAT 
TGGAATTTTT TCAGAAACCG CTATATCCCA GAAGGTGTTT CCTTCGATGT GGAGCCCAAT 
TTTGTACAAT ACACCAAAGG GGTAAAAGTT CCGCATTGTC ACAAAGTTTC AAAAATCATT 
ACGTTATTTA ACGACGAGAG TAATGATAAT AAGAAGAGGA CAATTGATAT GAACTATACT 
AAATGTTTGG CGAGAGGCAT GTTACGAGAC GAAAAAAAAT TTGTAAAAGT TAACGATAAA 
AGTCAAGTCG ATAACAACAG TGTCAACCAT GATTCATCCC AATCTTTTAC CTTATCGAAC 
GCAGAATTAG ATGACATACT GGGCTCAGAT TCTGATTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
Nature387(6632 Suppl)84-7(1997 May)
Churcher C,Bowman S,Badcock K,Bankier A,Brown D,Chillingworth T,Connor R,Devlin K,Gentles S,Hamlin N,Harris D,Horsnell T,Hunt S,Jagels K,Jones M,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Rowley N,Skelton J,Smith V,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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MPH1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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