RNR3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	RNR3
Entrez Gene ID	854744
Full Name	ribonucleotide-diphosphate reductase subunit RNR3
Synonyms	DIN1,RIR3
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179416.3	NP_012198.3	ribonucleotide-diphosphate reductase subunit RNR3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RRM1	XP_534027.2
Mus musculus (house mouse)	Rrm1	NP_033129.2
Gallus gallus (chicken)	RRM1	NP_001026008.1
Saccharomyces cerevisiae (baker's yeast)	RNR3	NP_012198.3
Eremothecium gossypii	AGOS_ADL057W	NP_984039.2
Schizosaccharomyces pombe (fission yeast)	cdc22	NP_594491.1
Pan troglodytes (chimpanzee)	RRM1	XP_001160029.1
Bos taurus (cattle)	RRM1	NP_001106767.1
Rattus norvegicus (Norway rat)	Rrm1	NP_001013254.1
Caenorhabditis elegans (roundworm)	rnr-1	NP_499039.1
Saccharomyces cerevisiae (baker's yeast)	RNR1	NP_010993.1
Oryza sativa (rice)	Os06g0168600	NP_001056926.1
Danio rerio (zebrafish)	rrm1	NP_571530.1
Magnaporthe oryzae (rice blast fungus)	MGG_07000	XP_003709756.1
Neurospora crassa	NCU03539	XP_956818.1
Xenopus tropicalis (tropical clawed frog)	rrm1	NP_001120566.1
Homo sapiens (human)	RRM1	NP_001024.1
	RRM1	XP_001113010.1
Drosophila melanogaster (fruit fly)	RnrL	NP_477027.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010198	XP_554103.3
Kluyveromyces lactis	KLLA0C07887g	XP_452551.1
Arabidopsis thaliana (thale cress)	RNR1	NP_179770.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
Churcher C, Bowman S, Badcock K, Bankier A, Brown D, Chillingworth T, Connor R, Devlin K, Gentles S, Hamlin N, Harris D, Horsnell T, Hunt S, Jagels K, Jones M, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Rowley N, Skelton J, Smith V, Barrell B
Nature387(6632 Suppl)84-7(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The gene encoding a large subunit of ribonucleotide reductase, RNR3, is regulated by ISW2 and SWI/SNF, complexes that repress and activate transcription, respectively. Here, we studied the functional interactions of these two complexes at RNR3.
Title: A novel mechanism of antagonism between ATP-dependent chromatin remodeling complexes regulates RNR3 expression.

RNR3 is dependent upon both TFIID and SAGA, two complexes that deliver TATA-binding protein (TBP) to promoters.
Title: Dissection of coactivator requirement at RNR3 reveals unexpected contributions from TFIID and SAGA.

Recruitment of Rap1 to the RNR3 gene is dependent on activation of the DNA damage checkpoint and chromatin remodelling by SWI/SNF.
Title: Yeast Rap1 contributes to genomic integrity by activating DNA damage repair genes.

nucleosome placement plays a dominant role in repression and that the ability of the core promoter to position a nucleosome is a major determinant in TAF(II) dependency of genes in vivo
Title: Exposing the core promoter is sufficient to activate transcription and alter coactivator requirement at RNR3.

Two histone deacetylases, RPD3 and HOS2, are required for the activation of DNA damage-inducible genes RNR3 and HUG1.
Title: Histone deacetylases RPD3 and HOS2 regulate the transcriptional activation of DNA damage-inducible genes.

The following RNR3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNR3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03772	NM_001179416.3 Latest version!	Saccharomyces cerevisiae S288C ribonucleotide-diphosphate reductase subunit RNR3 (RNR3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RNR3 ( NM_001179416.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012198.3 Saccharomyces cerevisiae S288C ribonucleotide-diphosphate reductase subunit RNR3 (RNR3), partial mRNA.

Price & Availability↑

CloneID	OSi03772
Clone ID Related Accession (Same CDS sequence)	NM_001179416.3
Accession Version	NM_001179416.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	ribonucleotide-diphosphate reductase subunit RNR3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001141). On Jul 30, 2012 this sequence version replaced NM_001179416.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTACGTTA TTAAAAGAGA CGGCCGCAAA GAGCCCGTTC AATTCGATAA AATTACCTCC 
CGTATCACCC GTTTGTCATA CGGTTTAGAC CCAAACCGTA TTGATGCTGT TAAGGTAACC 
CAACGTATTA TTTCTGGTGT GTACTCCGGT GTTACTACCG TTGAGCTGGA CAATCTTGCA 
GCTGAAACAT GTGCATACAT GACCACTGTG CACCCTGATT ATGCCACTCT AGCCGCTAGA 
ATCGCCATCT CTAACTTACA TAAGCAAACC ACAAAGCAAT TCTCCAAAGT TATTGAGGAT 
TTACACGACT GGATTAACCC AGCTACTGGA AAGCATGCTC CTATGATTTC GGACGAAATT 
TACAACATTG TCATGGAAAA CAAAGATACT TTGAACTCGG CCATCGTGTA CGATAGGGAT 
TTCCAGTATA CGTATTTCGG ATTCAAGACA CTGGAGCGTT CGTACTTGCT AAGACTGAAC 
GGTGAAGTGG CAGAACGTCC TCAGCATTTG GTAATGCGTG TGGCGCTAGG TATCCATGGT 
AGCGATATCG AATCTGTGCT GAAGACTTAT AATTTGATGT CGTTAAGATA CTTCACTCAC 
GCTTCCCCAA CTTTATTCAA CGCTGGTACG CCACATCCTC AAATGTCTTC ATGTTTCTTA 
ATTGCCATGA AGGATGACTC TATCGAAGGT ATTTATGATA CTTTGAAAGA ATGTGCTATG 
ATTTCCAAAA CTGCAGGTGG TGTTGGTCTT CATATCAACA ACATCCGTTC CACAGGTTCT 
TATATCGCTG GTACCAACGG TACTTCAAAC GGGTTGATTC CTATGATTCG TGTTTTCAAT 
AATACTGCCC GTTATGTGGA CCAGGGTGGT AACAAGAGAC CTGGTGCTTT CGCCCTTTTC 
TTGGAGCCAT GGCATGCAGA TATCTTCGAC TTTGTCGATA TCAGAAAAAC ACATGGTAAG 
GAAGAAATTC GTGCAAGAGA TTTGTTCCCT GCTCTATGGA TCCCTGATCT TTTCATGAAA 
CGTGTTCAAG AGGATGGGCC TTGGACTTTG TTTTCGCCCA GTGCTGCCCC AGGTTTAGAT 
GATGTGTGGG GTGATGAATT TGAAGAACTA TATACTCGTT ACGAAAGAGA AGGTCGTGGT 
AAAACAATTA AAGCCCAAAA GTTGTGGTAT GCCATTTTGC AAGCACAGAC AGAAACAGGT 
ACACCTTTCA TGGTTTATAA GGACGCATGT AACAGGAAGA CAAACCAACA GAACTTAGGT 
ACTATCAAAT CATCTAATTT ATGTTGTGAA ATCGTCGAAT ATTCCTCCCC GGATGAAACT 
GCAGTTTGTA ATTTAGCTTC TATTGCCCTA CCAGCATTCG TTGAGGTTTC AGAAGATGGT 
AAAACTGCAA GCTATAATTT CGAGAGATTA CACGAGATTG CTAAAGTCAT TACTCACAAC 
TTGAACAGAG TTATCGACCG TAATTACTAT CCAGTTCCCG AGGCTAGAAA TTCAAATATG 
AAGCATAGAC CTATTGCTCT TGGTGTCCAG GGTTTGGCCG ATACTTATAT GATGTTGCGT 
CTACCCTTTG AATCGGAAGA AGCTCAAACT CTAAACAAAC AAATCTTCGA AACTATTTAC 
CATGCTACTC TTGAAGCCTC CTGTGAATTG GCCCAAAAAG AAGGTAAATA TTCTACTTTT 
GAAGGTTCTC CAGCTTCTAA GGGTATTTTA CAATTCGATA TGTGGAACGC TAAACCATTT 
GGCATGTGGG ATTGGGAAAC CTTAAGAAAG GACATTGTTA AACATGGGTT AAGAAACTCT 
TTGACTATGG CACCAATGCC AACCGCCTCA ACTTCCCAAA TTCTTGGTTA TAATGAATGC 
TTCGAACCAG TGACCTCAAA CATGTACTCT CGTCGTGTCC TGTCTGGTGA ATTCCAAGTT 
GTTAATCCAT ATTTACTACG TGATTTAGTC GACCTGGGTA TTTGGGATGA TAGTATGAAA 
CAATATCTAA TTACACAAAA TGGTTCTATT CAAGGCTTAC CAAATGTGCC ACAAGAATTG 
AAGGAATTAT ACAAAACCGT CTGGGAAATC TCTCAAAAGA CCATTATCAA TATGGCTGCT 
GATCGTGCCA TCTACATCGA TCAGTCTCAT TCCTTGAATC TTTTCTTGCA AGCACCATCA 
ATGGGTAAGA TTACTAGTAT GCATTTCTAC GGTTGGAAGA AGGGTTTAAA AACTGGTATG 
TACTACTTAA GAACGCAAGC CGCCTCCGCT GCTATTCAAT TTACCATTGA TCAAGAGGTT 
GCCGATCAAG CCGCTACACA TATTGCTTCC GTCTCAGAAT TGGATCGTCC AGTTTATGTT 
CCAAAGGGTA CAAAATTCTC TGAACAAAAG GCGGCATCTG CGCTTACCGA AAGCTCAGAT 
AATGAGAAGG ATGCATCTCC AGTTCCATCC GAACAATCAT CGGTGTCGAG TGCCATGTCA 
AATGTGAAAT TGGAAGATAG TGTTGCCCCA GCAGTTCCAA CGGAAACAAT AAAAGAAGAT 
TCCGACGAGA AGAAATGTGA CATTTACAAT GAAAAGGTGA TTGCTTGTAC TGCTCCTACT 
CCAGAAGCTT GTGAGTCATG TTCCGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012198.3
CDS	1..2610
Translation	MYVIKRDGRKEPVQFDKITSRITRLSYGLDPNRIDAVKVTQRIISGVYSGVTTVELDNLAAETCAYMTTVHPDYATLAARIAISNLHKQTTKQFSKVIEDLHDWINPATGKHAPMISDEIYNIVMENKDTLNSAIVYDRDFQYTYFGFKTLERSYLLRLNGEVAERPQHLVMRVALGIHGSDIESVLKTYNLMSLRYFTHASPTLFNAGTPHPQMSSCFLIAMKDDSIEGIYDTLKECAMISKTAGGVGLHINNIRSTGSYIAGTNGTSNGLIPMIRVFNNTARYVDQGGNKRPGAFALFLEPWHADIFDFVDIRKTHGKEEIRARDLFPALWIPDLFMKRVQEDGPWTLFSPSAAPGLDDVWGDEFEELYTRYEREGRGKTIKAQKLWYAILQAQTETGTPFMVYKDACNRKTNQQNLGTIKSSNLCCEIVEYSSPDETAVCNLASIALPAFVEVSEDGKTASYNFERLHEIAKVITHNLNRVIDRNYYPVPEARNSNMKHRPIALGVQGLADTYMMLRLPFESEEAQTLNKQIFETIYHATLEASCELAQKEGKYSTFEGSPASKGILQFDMWNAKPFGMWDWETLRKDIVKHGLRNSLTMAPMPTASTSQILGYNECFEPVTSNMYSRRVLSGEFQVVNPYLLRDLVDLGIWDDSMKQYLITQNGSIQGLPNVPQELKELYKTVWEISQKTIINMAADRAIYIDQSHSLNLFLQAPSMGKITSMHFYGWKKGLKTGMYYLRTQAASAAIQFTIDQEVADQAATHIASVSELDRPVYVPKGTKFSEQKAASALTESSDNEKDASPVPSEQSSVSSAMSNVKLEDSVAPAVPTETIKEDSDEKKCDIYNEKVIACTAPTPEACESCSG

Target ORF information:

RefSeq Version	NM_001179416.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C ribonucleotide-diphosphate reductase subunit RNR3 (RNR3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179416.3

ORF Insert Sequence:

ATGTACGTTA TTAAAAGAGA CGGCCGCAAA GAGCCCGTTC AATTCGATAA AATTACCTCC 
CGTATCACCC GTTTGTCATA CGGTTTAGAC CCAAACCGTA TTGATGCTGT TAAGGTAACC 
CAACGTATTA TTTCTGGTGT GTACTCCGGT GTTACTACCG TTGAGCTGGA CAATCTTGCA 
GCTGAAACAT GTGCATACAT GACCACTGTG CACCCTGATT ATGCCACTCT AGCCGCTAGA 
ATCGCCATCT CTAACTTACA TAAGCAAACC ACAAAGCAAT TCTCCAAAGT TATTGAGGAT 
TTACACGACT GGATTAACCC AGCTACTGGA AAGCATGCTC CTATGATTTC GGACGAAATT 
TACAACATTG TCATGGAAAA CAAAGATACT TTGAACTCGG CCATCGTGTA CGATAGGGAT 
TTCCAGTATA CGTATTTCGG ATTCAAGACA CTGGAGCGTT CGTACTTGCT AAGACTGAAC 
GGTGAAGTGG CAGAACGTCC TCAGCATTTG GTAATGCGTG TGGCGCTAGG TATCCATGGT 
AGCGATATCG AATCTGTGCT GAAGACTTAT AATTTGATGT CGTTAAGATA CTTCACTCAC 
GCTTCCCCAA CTTTATTCAA CGCTGGTACG CCACATCCTC AAATGTCTTC ATGTTTCTTA 
ATTGCCATGA AGGATGACTC TATCGAAGGT ATTTATGATA CTTTGAAAGA ATGTGCTATG 
ATTTCCAAAA CTGCAGGTGG TGTTGGTCTT CATATCAACA ACATCCGTTC CACAGGTTCT 
TATATCGCTG GTACCAACGG TACTTCAAAC GGGTTGATTC CTATGATTCG TGTTTTCAAT 
AATACTGCCC GTTATGTGGA CCAGGGTGGT AACAAGAGAC CTGGTGCTTT CGCCCTTTTC 
TTGGAGCCAT GGCATGCAGA TATCTTCGAC TTTGTCGATA TCAGAAAAAC ACATGGTAAG 
GAAGAAATTC GTGCAAGAGA TTTGTTCCCT GCTCTATGGA TCCCTGATCT TTTCATGAAA 
CGTGTTCAAG AGGATGGGCC TTGGACTTTG TTTTCGCCCA GTGCTGCCCC AGGTTTAGAT 
GATGTGTGGG GTGATGAATT TGAAGAACTA TATACTCGTT ACGAAAGAGA AGGTCGTGGT 
AAAACAATTA AAGCCCAAAA GTTGTGGTAT GCCATTTTGC AAGCACAGAC AGAAACAGGT 
ACACCTTTCA TGGTTTATAA GGACGCATGT AACAGGAAGA CAAACCAACA GAACTTAGGT 
ACTATCAAAT CATCTAATTT ATGTTGTGAA ATCGTCGAAT ATTCCTCCCC GGATGAAACT 
GCAGTTTGTA ATTTAGCTTC TATTGCCCTA CCAGCATTCG TTGAGGTTTC AGAAGATGGT 
AAAACTGCAA GCTATAATTT CGAGAGATTA CACGAGATTG CTAAAGTCAT TACTCACAAC 
TTGAACAGAG TTATCGACCG TAATTACTAT CCAGTTCCCG AGGCTAGAAA TTCAAATATG 
AAGCATAGAC CTATTGCTCT TGGTGTCCAG GGTTTGGCCG ATACTTATAT GATGTTGCGT 
CTACCCTTTG AATCGGAAGA AGCTCAAACT CTAAACAAAC AAATCTTCGA AACTATTTAC 
CATGCTACTC TTGAAGCCTC CTGTGAATTG GCCCAAAAAG AAGGTAAATA TTCTACTTTT 
GAAGGTTCTC CAGCTTCTAA GGGTATTTTA CAATTCGATA TGTGGAACGC TAAACCATTT 
GGCATGTGGG ATTGGGAAAC CTTAAGAAAG GACATTGTTA AACATGGGTT AAGAAACTCT 
TTGACTATGG CACCAATGCC AACCGCCTCA ACTTCCCAAA TTCTTGGTTA TAATGAATGC 
TTCGAACCAG TGACCTCAAA CATGTACTCT CGTCGTGTCC TGTCTGGTGA ATTCCAAGTT 
GTTAATCCAT ATTTACTACG TGATTTAGTC GACCTGGGTA TTTGGGATGA TAGTATGAAA 
CAATATCTAA TTACACAAAA TGGTTCTATT CAAGGCTTAC CAAATGTGCC ACAAGAATTG 
AAGGAATTAT ACAAAACCGT CTGGGAAATC TCTCAAAAGA CCATTATCAA TATGGCTGCT 
GATCGTGCCA TCTACATCGA TCAGTCTCAT TCCTTGAATC TTTTCTTGCA AGCACCATCA 
ATGGGTAAGA TTACTAGTAT GCATTTCTAC GGTTGGAAGA AGGGTTTAAA AACTGGTATG 
TACTACTTAA GAACGCAAGC CGCCTCCGCT GCTATTCAAT TTACCATTGA TCAAGAGGTT 
GCCGATCAAG CCGCTACACA TATTGCTTCC GTCTCAGAAT TGGATCGTCC AGTTTATGTT 
CCAAAGGGTA CAAAATTCTC TGAACAAAAG GCGGCATCTG CGCTTACCGA AAGCTCAGAT 
AATGAGAAGG ATGCATCTCC AGTTCCATCC GAACAATCAT CGGTGTCGAG TGCCATGTCA 
AATGTGAAAT TGGAAGATAG TGTTGCCCCA GCAGTTCCAA CGGAAACAAT AAAAGAAGAT 
TCCGACGAGA AGAAATGTGA CATTTACAAT GAAAAGGTGA TTGCTTGTAC TGCTCCTACT 
CCAGAAGCTT GTGAGTCATG TTCCGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
Nature387(6632 Suppl)84-7(1997 May)
Churcher C,Bowman S,Badcock K,Bankier A,Brown D,Chillingworth T,Connor R,Devlin K,Gentles S,Hamlin N,Harris D,Horsnell T,Hunt S,Jagels K,Jones M,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Rowley N,Skelton J,Smith V,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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RNR3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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