MCM10 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MCM10
Entrez Gene ID	854656
Full Name	Mcm10p
Synonyms	DNA43
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179498.1	NP_012116.1	Mcm10p

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AFR287W	NP_985834.1
Saccharomyces cerevisiae (baker's yeast)	MCM10	NP_012116.1
Kluyveromyces lactis	KLLA0A00572g	XP_451023.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
Churcher C, Bowman S, Badcock K, Bankier A, Brown D, Chillingworth T, Connor R, Devlin K, Gentles S, Hamlin N, Harris D, Horsnell T, Hunt S, Jagels K, Jones M, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Rowley N, Skelton J, Smith V, Barrell B
Nature387(6632 Suppl)84-7(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

An intact Mcm10 coiled-coil interaction surface is important for origin melting, helicase assembly and the recruitment of Pol-alpha to Mcm2-7.
Title: An intact Mcm10 coiled-coil interaction surface is important for origin melting, helicase assembly and the recruitment of Pol-? to Mcm2-7.

These data suggest that DNA binding by Mcm10 is essential for the initiation of DNA replication.
Title: An Mcm10 Mutant Defective in ssDNA Binding Shows Defects in DNA Replication Initiation.

Mcm10 directly binds the Minichromosome Maintenance Complex via Mcm2 and the Mcm6 C terminus.
Title: Recruitment of Mcm10 to Sites of Replication Initiation Requires Direct Binding to the Minichromosome Maintenance (MCM) Complex.

Mcm10 deficiency causes defective-replisome-induced mutagenesis and a dependency on error-free postreplicative repair.
Title: Mcm10 deficiency causes defective-replisome-induced mutagenesis and a dependency on error-free postreplicative repair.

In the absence of Mcm10, a stable Cdc45-Mcm-GINS (CMG) complex was assembled at origins. However subsequent translocation of CMG, replication protein A loading to origins, and the intra-S checkpoint activation were severely diminished.
Title: Mcm10 plays a role in functioning of the eukaryotic replicative DNA helicase, Cdc45-Mcm-GINS.

The following MCM10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MCM10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03693	NM_001179498.1 Latest version!	Saccharomyces cerevisiae S288C Mcm10p (MCM10), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MCM10 ( NM_001179498.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012116.1 Saccharomyces cerevisiae S288C Mcm10p (MCM10), partial mRNA.

Price & Availability↑

CloneID	OSi03693
Clone ID Related Accession (Same CDS sequence)	NM_001179498.1
Accession Version	NM_001179498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Mcm10p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001141). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAATGATC CTCGTGAAAT TTTAGCGGTT GATCCGTACA ATAATATTAC TTCTGATGAA 
GAGGATGAGC AAGCCATCGC GAGAGAACTT GAATTTATGG AACGAAAGAG GCAGGCCTTA 
GTGGAACGAT TAAAAAGAAA GCAAGAATTT AAGAAACCCC AGGATCCTAA TTTTGAAGCC 
ATCGAGGTAC CTCAATCTCC TACCAAAAAC CGTGTGAAAG TGGGGTCTCA TAATGCTACA 
CAACAAGGCA CAAAATTCGA AGGTTCGAAT ATTAATGAAG TAAGGTTATC TCAATTACAG 
CAGCAACCAA AACCACCAGC TAGTACAACC ACATACTTTA TGGAGAAATT TCAAAACGCA 
AAGAAGAACG AAGATAAACA AATTGCCAAG TTTGAAAGCA TGATGAATGC AAGAGTACAT 
ACGTTCAGTA CCGATGAGAA GAAATATGTG CCGATAATCA CAAACGAATT AGAAAGCTTT 
TCAAATCTTT GGGTTAAAAA GAGGTACATA CCTGAAGATG ACTTAAAACG GGCTTTGCAT 
GAGATCAAAA TCCTTCGGTT GGGCAAACTT TTTGCTAAAA TTCGCCCACC TAAATTTCAA 
GAGCCTGAAT ACGCCAACTG GGCCACCGTA GGCCTCATTA GCCACAAATC GGACATCAAA 
TTTACATCAT CTGAAAAGCC AGTCAAATTC TTCATGTTCA CCATAACGGA CTTTCAGCAT 
ACACTAGATG TTTATATCTT CGGGAAAAAG GGTGTAGAAA GATATTATAA TCTTCGCCTG 
GGTGATGTGA TAGCAATATT AAACCCAGAA GTACTACCAT GGAGACCCTC AGGGCGAGGA 
AATTTTATCA AATCCTTCAA CCTTCGAATT AGTCATGACT TCAAATGTAT CCTGGAGATA 
GGTTCAAGTA GAGATTTAGG TTGGTGTCCC ATAGTGAATA AAAAGACTCA CAAAAAATGT 
GGCTCTCCCA TTAACATATC TCTTCATAAG TGTTGCGATT ACCATAGAGA AGTGCAATTT 
CGTGGAACAA GTGCTAAAAG AATTGAATTA AATGGTGGGT ACGCCTTGGG CGCGCCTACG 
AAAGTGGACT CTCAACCAAG CCTATATAAG GCCAAAGGGG AAAACGGGTT TAATATAATC 
AAAGGTACTC GTAAGCGCCT GTCAGAAGAG GAGGAAAGAC TTAAAAAGAG CTCTCACAAT 
TTTACGAATA GTAATTCTGC CAAAGCATTT TTCGACGAGA AATTTCAGAA TCCAGATATG 
CTGGCAAACT TAGACAATAA AAGAAGGAAA ATAATAGAAA CTAAGAAATC GACAGCACTG 
AGCCGCGAAC TAGGCAAAAT TATGAGAAGG AGGGAATCCA GCGGATTAGA AGATAAGAGC 
GTCGGAGAGC GACAGAAAAT GAAACGAACC ACAGAAAGTG CCCTCCAGAC AGGGCTTATC 
CAACGCCTAG GATTCGATCC CACTCATGGA AAAATTTCCC AAGTACTCAA GTCTTCTGTA 
TCAGGGAGCG AACCTAAGAA CAACTTACTC GGTAAAAAAA AAACTGTTAT AAACGATCTC 
TTGCATTACA AGAAGGAAAA AGTCATTCTC GCACCTTCAA AGAACGAATG GTTCAAGAAA 
AGAAGCCATC GCGAAGAAGT TTGGCAAAAA CATTTCGGAT CCAAGGAAAC TAAAGAAACT 
TCTGACGGTA GTGCCAGCGA TCTTGAGATA ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012116.1
CDS	1..1716
Translation	MNDPREILAVDPYNNITSDEEDEQAIARELEFMERKRQALVERLKRKQEFKKPQDPNFEAIEVPQSPTKNRVKVGSHNATQQGTKFEGSNINEVRLSQLQQQPKPPASTTTYFMEKFQNAKKNEDKQIAKFESMMNARVHTFSTDEKKYVPIITNELESFSNLWVKKRYIPEDDLKRALHEIKILRLGKLFAKIRPPKFQEPEYANWATVGLISHKSDIKFTSSEKPVKFFMFTITDFQHTLDVYIFGKKGVERYYNLRLGDVIAILNPEVLPWRPSGRGNFIKSFNLRISHDFKCILEIGSSRDLGWCPIVNKKTHKKCGSPINISLHKCCDYHREVQFRGTSAKRIELNGGYALGAPTKVDSQPSLYKAKGENGFNIIKGTRKRLSEEEERLKKSSHNFTNSNSAKAFFDEKFQNPDMLANLDNKRRKIIETKKSTALSRELGKIMRRRESSGLEDKSVGERQKMKRTTESALQTGLIQRLGFDPTHGKISQVLKSSVSGSEPKNNLLGKKKTVINDLLHYKKEKVILAPSKNEWFKKRSHREEVWQKHFGSKETKETSDGSASDLEII

Target ORF information:

RefSeq Version	NM_001179498.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Mcm10p (MCM10), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179498.1

ORF Insert Sequence:

ATGAATGATC CTCGTGAAAT TTTAGCGGTT GATCCGTACA ATAATATTAC TTCTGATGAA 
GAGGATGAGC AAGCCATCGC GAGAGAACTT GAATTTATGG AACGAAAGAG GCAGGCCTTA 
GTGGAACGAT TAAAAAGAAA GCAAGAATTT AAGAAACCCC AGGATCCTAA TTTTGAAGCC 
ATCGAGGTAC CTCAATCTCC TACCAAAAAC CGTGTGAAAG TGGGGTCTCA TAATGCTACA 
CAACAAGGCA CAAAATTCGA AGGTTCGAAT ATTAATGAAG TAAGGTTATC TCAATTACAG 
CAGCAACCAA AACCACCAGC TAGTACAACC ACATACTTTA TGGAGAAATT TCAAAACGCA 
AAGAAGAACG AAGATAAACA AATTGCCAAG TTTGAAAGCA TGATGAATGC AAGAGTACAT 
ACGTTCAGTA CCGATGAGAA GAAATATGTG CCGATAATCA CAAACGAATT AGAAAGCTTT 
TCAAATCTTT GGGTTAAAAA GAGGTACATA CCTGAAGATG ACTTAAAACG GGCTTTGCAT 
GAGATCAAAA TCCTTCGGTT GGGCAAACTT TTTGCTAAAA TTCGCCCACC TAAATTTCAA 
GAGCCTGAAT ACGCCAACTG GGCCACCGTA GGCCTCATTA GCCACAAATC GGACATCAAA 
TTTACATCAT CTGAAAAGCC AGTCAAATTC TTCATGTTCA CCATAACGGA CTTTCAGCAT 
ACACTAGATG TTTATATCTT CGGGAAAAAG GGTGTAGAAA GATATTATAA TCTTCGCCTG 
GGTGATGTGA TAGCAATATT AAACCCAGAA GTACTACCAT GGAGACCCTC AGGGCGAGGA 
AATTTTATCA AATCCTTCAA CCTTCGAATT AGTCATGACT TCAAATGTAT CCTGGAGATA 
GGTTCAAGTA GAGATTTAGG TTGGTGTCCC ATAGTGAATA AAAAGACTCA CAAAAAATGT 
GGCTCTCCCA TTAACATATC TCTTCATAAG TGTTGCGATT ACCATAGAGA AGTGCAATTT 
CGTGGAACAA GTGCTAAAAG AATTGAATTA AATGGTGGGT ACGCCTTGGG CGCGCCTACG 
AAAGTGGACT CTCAACCAAG CCTATATAAG GCCAAAGGGG AAAACGGGTT TAATATAATC 
AAAGGTACTC GTAAGCGCCT GTCAGAAGAG GAGGAAAGAC TTAAAAAGAG CTCTCACAAT 
TTTACGAATA GTAATTCTGC CAAAGCATTT TTCGACGAGA AATTTCAGAA TCCAGATATG 
CTGGCAAACT TAGACAATAA AAGAAGGAAA ATAATAGAAA CTAAGAAATC GACAGCACTG 
AGCCGCGAAC TAGGCAAAAT TATGAGAAGG AGGGAATCCA GCGGATTAGA AGATAAGAGC 
GTCGGAGAGC GACAGAAAAT GAAACGAACC ACAGAAAGTG CCCTCCAGAC AGGGCTTATC 
CAACGCCTAG GATTCGATCC CACTCATGGA AAAATTTCCC AAGTACTCAA GTCTTCTGTA 
TCAGGGAGCG AACCTAAGAA CAACTTACTC GGTAAAAAAA AAACTGTTAT AAACGATCTC 
TTGCATTACA AGAAGGAAAA AGTCATTCTC GCACCTTCAA AGAACGAATG GTTCAAGAAA 
AGAAGCCATC GCGAAGAAGT TTGGCAAAAA CATTTCGGAT CCAAGGAAAC TAAAGAAACT 
TCTGACGGTA GTGCCAGCGA TCTTGAGATA ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
Nature387(6632 Suppl)84-7(1997 May)
Churcher C,Bowman S,Badcock K,Bankier A,Brown D,Chillingworth T,Connor R,Devlin K,Gentles S,Hamlin N,Harris D,Horsnell T,Hunt S,Jagels K,Jones M,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Rowley N,Skelton J,Smith V,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

MCM10 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報