PCK1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PCK1
Entrez Gene ID	853972
Full Name	phosphoenolpyruvate carboxykinase PCK1
Synonyms	JPM2,PPC1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179887.3	NP_013023.3	phosphoenolpyruvate carboxykinase PCK1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	PCK1	NP_013023.3
Kluyveromyces lactis	KLLA0A00484g	XP_451019.1
Magnaporthe oryzae (rice blast fungus)	MGG_00450	XP_003718617.1
Eremothecium gossypii	AGOS_AFR292W	NP_985839.1
Neurospora crassa	NCU09873	XP_960155.1
Arabidopsis thaliana (thale cress)	PCK1	NP_195500.1
Oryza sativa (rice)	Os03g0255500	NP_001049595.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome XI.
Dujon B, Alexandraki D, Andr? B, Ansorge W, Baladron V, Ballesta JP, Banrevi A, Bolle PA, Bolotin-Fukuhara M, Bossier P, Bou G, Boyer J, Bultrago MJ, Cheret G, Colleaux L, Dalgnan-Fornler B, del Rey F, Dlon C, Domdey H, D?sterhoft A, D?sterhus S, Entlan KD, Erfle H, Esteban PF, Feldmann H, Fernandes L, Robo GM, Fritz C, Fukuhara H, Gabel C, Gaillon L, Carcia-Cantalejo JM, Garcia-Ramirez JJ, Gent NE, Ghazvini M, Goffeau A, Gonzal?z A, Grothues D, Guerreiro P, Hegemann J, Hewitt N, Hilger F, Hollenberg CP, Horaitis O, Indge KJ, Jacquier A, James CM, Jauniaux C, Jimenez A, Keuchel H, Kirchrath L, Kleine K, K?tter P, Legrain P, Liebl S, Louis EJ, Maia e Silva A, Marck C, Monnier AL, M?stl D, M?ller S, Obermaier B, Oliver SG, Pallier C, Pascolo S, Pfeiffer F, Philippsen P, Planta RJ, Pohl FM, Pohl TM, P?hlmann R, Portetelle D, Purnelle B, Puzos V, Ramezani Rad M, Rasmussen SW, Remacha M, Revuelta JL, Richard GF, Rieger M, Rodrigues-Pousada C, Rose M, Rupp T, Santos MA, Schwager C, Sensen C, Skala J, Soares H, Sor F, Stegemann J, Tettelin H, Thierry A, Tzermia M, Urrestarazu LA, van Dyck L, Van Vliet-Reedijk JC, Valens M, Vandenbo M, Vilela C, Vissers S, von Wettstein D, Voss H, Wiemann S, Xu G, Zimmermann J, Haasemann M, Becker I, Mewes HW
Nature369(6479)371-8(1994 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Functional evaluation of serine 252 of Saccharomyces cerevisiae PCK1 is reported.
Title: Functional evaluation of serine 252 of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase.

Arg457 is an important factor for nucleotide binding by S. cerevisiae PEP carboxykinase.
Title: Relevance of Arg457 for the nucleotide affinity of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase.

Introduction of a hydroxyl group in the lateral chain of the residue (Phe216Tyr)produces a substantial loss in the enzyme affinity for Mn(2+).
Title: Relevance of phenylalanine 216 in the affinity of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase for Mn(II).

neighboring Phe(416) contributes to provide a low polarity microenvironment suitable to keep the epsilon-NH(2) of Lys(213) in the unprotonated form
Title: Site-directed mutagenesis study of the microenvironment characteristics of Lys213 of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase.

the electrostatic interaction between the guanidinium group of Arg70 and the carboxyl group of PEP is important for PEP binding by phosphoenolpyruvate (PEP) carboxykinase and for further steps in catalysis.
Title: Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase: relevance of arginine 70 for catalysis.

The following PCK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03149	NM_001179887.3 Latest version!	Saccharomyces cerevisiae S288C phosphoenolpyruvate carboxykinase PCK1 (PCK1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PCK1 ( NM_001179887.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013023.3 Saccharomyces cerevisiae S288C phosphoenolpyruvate carboxykinase PCK1 (PCK1), partial mRNA.

Price & Availability↑

CloneID	OSi03149
Clone ID Related Accession (Same CDS sequence)	NM_001179887.3
Accession Version	NM_001179887.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	phosphoenolpyruvate carboxykinase PCK1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001143). On Jul 30, 2012 this sequence version replaced NM_001179887.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCCCCTT CTAAAATGAA TGCTACAGTA GGATCTACTT CCGAAGTTGA ACAAAAAATC 
AGACAAGAAT TGGCTCTTAG TGACGAAGTC ACCACCATCA GACGCAATGC TCCAGCTGCC 
GTTTTGTATG AAGATGGTCT AAAAGAAAAT AAAACTGTCA TTTCATCAAG CGGTGCATTG 
ATCGCTTATT CCGGTGTTAA AACCGGAAGA TCTCCAAAGG ACAAACGTAT TGTTGAAGAA 
CCTACCTCGA AAGACGAAAT TTGGTGGGGT CCGGTCAATA AACCATGTTC TGAAAGAACA 
TGGTCTATCA ACCGTGAAAG AGCTGCAGAT TACTTGAGAA CAAGAGACCA CATTTATATT 
GTCGATGCAT TTGCAGGATG GGATCCAAAA TACAGAATCA AAGTCCGCGT TGTTTGTGCC 
AGGGCTTACC ACGCTTTATT CATGACAAAT ATGCTTATTA GACCTACAGA AGAAGAATTA 
GCCCATTTTG GAGAACCTGA TTTTACTGTC TGGAACGCTG GTCAGTTCCC AGCCAATTTA 
CACACCCAGG ATATGTCTTC AAAGAGTACT ATAGAAATTA ACTTCAAAGC AATGGAAATG 
ATCATTTTAG GTACCGAATA CGCCGGTGAA ATGAAAAAAG GTATTTTCAC AGTTATGTTT 
TACTTGATGC CTGTGCACCA TAACGTTTTA ACTTTGCACT CTTCCGCCAA CCAGGGTATT 
CAAAACGGTG ACGTTACTTT ATTCTTTGGC CTAAGTGGTA CCGGGAAAAC CACTTTATCC 
GCAGACCCAC ATAGATTGTT GATCGGCGAT GATGAACATT GTTGGTCCGA CCATGGTGTC 
TTCAATATCG AAGGTGGTTG TTACGCCAAG TGTATTAATT TATCTGCCGA AAAGGAGCCT 
GAAATTTTCG ACGCTATCAA GTTTGGTTCT GTATTAGAAA ACGTTATCTA TGACGAGAAG 
TCGCATGTAG TCGACTATGA CGACTCTTCT ATTACTGAAA ATACTAGATG TGCCTACCCA 
ATTGACTACA TTCCAAGTGC CAAGATTCCA TGTTTGGCGG ACTCTCATCC AAAGAACATT 
ATCCTGCTAA CTTGTGATGC TTCGGGTGTT TTACCACCAG TATCTAAATT GACTCCTGAA 
CAAGTCATGT ACCATTTCAT CTCTGGTTAC ACTTCTAAAA TGGCTGGTAC TGAGCAAGGT 
GTCACTGAAC CTGAACCAAC ATTTTCATCT TGTTTCGGAC AACCCTTCCT AGCCTTGCAC 
CCTATTAGAT ACGCAACCAT GTTAGCTACA AAGATGTCTC AACATAAAGC TAATGCGTAC 
TTAATCAACA CCGGCTGGAC TGGTTCTTCC TACGTATCTG GTGGTAAACG TTGCCCATTG 
AAGTACACAA GGGCCATTCT GGATTCTATT CATGATGGTT CGTTAGCCAA TGAAACGTAC 
GAAACTTTAC CGATTTTCAA TCTTCAAGTA CCTACCAAGG TTAACGGTGT TCCAGCTGAG 
CTTTTGAATC CTGCTAAAAA CTGGTCTCAA GGTGAATCCA AATACAGAGG TGCAGTTACC 
AACTTGGCCA ACTTGTTTGT TCAAAATTTC AAGATTTATC AAGACAGAGC CACACCAGAT 
GTATTAGCCG CTGGTCCTCA ATTCGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013023.3
CDS	1..1650
Translation	MSPSKMNATVGSTSEVEQKIRQELALSDEVTTIRRNAPAAVLYEDGLKENKTVISSSGALIAYSGVKTGRSPKDKRIVEEPTSKDEIWWGPVNKPCSERTWSINRERAADYLRTRDHIYIVDAFAGWDPKYRIKVRVVCARAYHALFMTNMLIRPTEEELAHFGEPDFTVWNAGQFPANLHTQDMSSKSTIEINFKAMEMIILGTEYAGEMKKGIFTVMFYLMPVHHNVLTLHSSANQGIQNGDVTLFFGLSGTGKTTLSADPHRLLIGDDEHCWSDHGVFNIEGGCYAKCINLSAEKEPEIFDAIKFGSVLENVIYDEKSHVVDYDDSSITENTRCAYPIDYIPSAKIPCLADSHPKNIILLTCDASGVLPPVSKLTPEQVMYHFISGYTSKMAGTEQGVTEPEPTFSSCFGQPFLALHPIRYATMLATKMSQHKANAYLINTGWTGSSYVSGGKRCPLKYTRAILDSIHDGSLANETYETLPIFNLQVPTKVNGVPAELLNPAKNWSQGESKYRGAVTNLANLFVQNFKIYQDRATPDVLAAGPQFE

Target ORF information:

RefSeq Version	NM_001179887.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C phosphoenolpyruvate carboxykinase PCK1 (PCK1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179887.3

ORF Insert Sequence:

ATGTCCCCTT CTAAAATGAA TGCTACAGTA GGATCTACTT CCGAAGTTGA ACAAAAAATC 
AGACAAGAAT TGGCTCTTAG TGACGAAGTC ACCACCATCA GACGCAATGC TCCAGCTGCC 
GTTTTGTATG AAGATGGTCT AAAAGAAAAT AAAACTGTCA TTTCATCAAG CGGTGCATTG 
ATCGCTTATT CCGGTGTTAA AACCGGAAGA TCTCCAAAGG ACAAACGTAT TGTTGAAGAA 
CCTACCTCGA AAGACGAAAT TTGGTGGGGT CCGGTCAATA AACCATGTTC TGAAAGAACA 
TGGTCTATCA ACCGTGAAAG AGCTGCAGAT TACTTGAGAA CAAGAGACCA CATTTATATT 
GTCGATGCAT TTGCAGGATG GGATCCAAAA TACAGAATCA AAGTCCGCGT TGTTTGTGCC 
AGGGCTTACC ACGCTTTATT CATGACAAAT ATGCTTATTA GACCTACAGA AGAAGAATTA 
GCCCATTTTG GAGAACCTGA TTTTACTGTC TGGAACGCTG GTCAGTTCCC AGCCAATTTA 
CACACCCAGG ATATGTCTTC AAAGAGTACT ATAGAAATTA ACTTCAAAGC AATGGAAATG 
ATCATTTTAG GTACCGAATA CGCCGGTGAA ATGAAAAAAG GTATTTTCAC AGTTATGTTT 
TACTTGATGC CTGTGCACCA TAACGTTTTA ACTTTGCACT CTTCCGCCAA CCAGGGTATT 
CAAAACGGTG ACGTTACTTT ATTCTTTGGC CTAAGTGGTA CCGGGAAAAC CACTTTATCC 
GCAGACCCAC ATAGATTGTT GATCGGCGAT GATGAACATT GTTGGTCCGA CCATGGTGTC 
TTCAATATCG AAGGTGGTTG TTACGCCAAG TGTATTAATT TATCTGCCGA AAAGGAGCCT 
GAAATTTTCG ACGCTATCAA GTTTGGTTCT GTATTAGAAA ACGTTATCTA TGACGAGAAG 
TCGCATGTAG TCGACTATGA CGACTCTTCT ATTACTGAAA ATACTAGATG TGCCTACCCA 
ATTGACTACA TTCCAAGTGC CAAGATTCCA TGTTTGGCGG ACTCTCATCC AAAGAACATT 
ATCCTGCTAA CTTGTGATGC TTCGGGTGTT TTACCACCAG TATCTAAATT GACTCCTGAA 
CAAGTCATGT ACCATTTCAT CTCTGGTTAC ACTTCTAAAA TGGCTGGTAC TGAGCAAGGT 
GTCACTGAAC CTGAACCAAC ATTTTCATCT TGTTTCGGAC AACCCTTCCT AGCCTTGCAC 
CCTATTAGAT ACGCAACCAT GTTAGCTACA AAGATGTCTC AACATAAAGC TAATGCGTAC 
TTAATCAACA CCGGCTGGAC TGGTTCTTCC TACGTATCTG GTGGTAAACG TTGCCCATTG 
AAGTACACAA GGGCCATTCT GGATTCTATT CATGATGGTT CGTTAGCCAA TGAAACGTAC 
GAAACTTTAC CGATTTTCAA TCTTCAAGTA CCTACCAAGG TTAACGGTGT TCCAGCTGAG 
CTTTTGAATC CTGCTAAAAA CTGGTCTCAA GGTGAATCCA AATACAGAGG TGCAGTTACC 
AACTTGGCCA ACTTGTTTGT TCAAAATTTC AAGATTTATC AAGACAGAGC CACACCAGAT 
GTATTAGCCG CTGGTCCTCA ATTCGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome XI.
Nature369(6479)371-8(1994 Jun)
Dujon B,Alexandraki D,Andr? B,Ansorge W,Baladron V,Ballesta JP,Banrevi A,Bolle PA,Bolotin-Fukuhara M,Bossier P,Bou G,Boyer J,Bultrago MJ,Cheret G,Colleaux L,Dalgnan-Fornler B,del Rey F,Dlon C,Domdey H,D?sterhoft A,D?sterhus S,Entlan KD,Erfle H,Esteban PF,Feldmann H,Fernandes L,Robo GM,Fritz C,Fukuhara H,Gabel C,Gaillon L,Carcia-Cantalejo JM,Garcia-Ramirez JJ,Gent NE,Ghazvini M,Goffeau A,Gonzal?z A,Grothues D,Guerreiro P,Hegemann J,Hewitt N,Hilger F,Hollenberg CP,Horaitis O,Indge KJ,Jacquier A,James CM,Jauniaux C,Jimenez A,Keuchel H,Kirchrath L,Kleine K,K?tter P,Legrain P,Liebl S,Louis EJ,Maia e Silva A,Marck C,Monnier AL,M?stl D,M?ller S,Obermaier B,Oliver SG,Pallier C,Pascolo S,Pfeiffer F,Philippsen P,Planta RJ,Pohl FM,Pohl TM,P?hlmann R,Portetelle D,Purnelle B,Puzos V,Ramezani Rad M,Rasmussen SW,Remacha M,Revuelta JL,Richard GF,Rieger M,Rodrigues-Pousada C,Rose M,Rupp T,Santos MA,Schwager C,Sensen C,Skala J,Soares H,Sor F,Stegemann J,Tettelin H,Thierry A,Tzermia M,Urrestarazu LA,van Dyck L,Van Vliet-Reedijk JC,Valens M,Vandenbo M,Vilela C,Vissers S,von Wettstein D,Voss H,Wiemann S,Xu G,Zimmermann J,Haasemann M,Becker I,Mewes HW

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PCK1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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