MIF2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MIF2
Entrez Gene ID	853773
Full Name	Mif2p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179655.1	NP_012834.1	Mif2p

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	MIF2	NP_012834.1
Kluyveromyces lactis	KLLA0B13629g	XP_452138.1
Eremothecium gossypii	AGOS_ABR015C	NP_982961.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome XI.
Dujon B, Alexandraki D, Andr? B, Ansorge W, Baladron V, Ballesta JP, Banrevi A, Bolle PA, Bolotin-Fukuhara M, Bossier P, Bou G, Boyer J, Bultrago MJ, Cheret G, Colleaux L, Dalgnan-Fornler B, del Rey F, Dlon C, Domdey H, D?sterhoft A, D?sterhus S, Entlan KD, Erfle H, Esteban PF, Feldmann H, Fernandes L, Robo GM, Fritz C, Fukuhara H, Gabel C, Gaillon L, Carcia-Cantalejo JM, Garcia-Ramirez JJ, Gent NE, Ghazvini M, Goffeau A, Gonzal?z A, Grothues D, Guerreiro P, Hegemann J, Hewitt N, Hilger F, Hollenberg CP, Horaitis O, Indge KJ, Jacquier A, James CM, Jauniaux C, Jimenez A, Keuchel H, Kirchrath L, Kleine K, K?tter P, Legrain P, Liebl S, Louis EJ, Maia e Silva A, Marck C, Monnier AL, M?stl D, M?ller S, Obermaier B, Oliver SG, Pallier C, Pascolo S, Pfeiffer F, Philippsen P, Planta RJ, Pohl FM, Pohl TM, P?hlmann R, Portetelle D, Purnelle B, Puzos V, Ramezani Rad M, Rasmussen SW, Remacha M, Revuelta JL, Richard GF, Rieger M, Rodrigues-Pousada C, Rose M, Rupp T, Santos MA, Schwager C, Sensen C, Skala J, Soares H, Sor F, Stegemann J, Tettelin H, Thierry A, Tzermia M, Urrestarazu LA, van Dyck L, Van Vliet-Reedijk JC, Valens M, Vandenbo M, Vilela C, Vissers S, von Wettstein D, Voss H, Wiemann S, Xu G, Zimmermann J, Haasemann M, Becker I, Mewes HW
Nature369(6479)371-8(1994 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mif2p dimer seems to be part of an enhanceosome-like structure that nucleates kinetochore assembly in budding yeast
Title: Structural and functional dissection of Mif2p, a conserved DNA-binding kinetochore protein.

The following MIF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MIF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02974	NM_001179655.1 Latest version!	Saccharomyces cerevisiae S288C Mif2p (MIF2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MIF2 ( NM_001179655.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012834.1 Saccharomyces cerevisiae S288C Mif2p (MIF2), partial mRNA.

Price & Availability↑

CloneID	OSi02974
Clone ID Related Accession (Same CDS sequence)	NM_001179655.1
Accession Version	NM_001179655.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Mif2p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001143). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGATTATA TGAAATTGGG GCTTAAGTCC CGTAAAACTG GTATTGATGT TAAGCAAGAT 
ATACCCAAAG ATGAATACAG CATGGAAAAT ATTGATGATT TTTTCAAAGA TGATGAAACT 
AGTCTTATCA GTATGAGAAG GAAAAGCAGA AGAAAATCAT CGCTTTTCTT ACCATCAACG 
TTAAATGGCG ATACTAAGAA CGTATTACCG CCATTTCTAC AGTCATATAA ATCTCAAGAT 
GATGAAGTTG TCCAAAGCCC ATCTGGGAAA GGCGATGGAT CAAGACGATC ATCTTTGTTA 
AGCCATCAAT CAAACTTCCT GAGTCCAGCC AATGATTTCG AGCCTATTGA GGAAGAACCA 
GAACAAGAAG AAAATGATAT CAGAGGCAAT GATTTTGCCA CACCAATCAC ACAGAAATTG 
AGTAAACCTA CATATAAAAG AAAGTACTCC ACTCGGTATA GCCTTGACAC TTCAGAAAGC 
CCTTCTGTAA GGTTGACACC TGATAGAATC ACTAATAAGA ATGTTTATTC AGATGTACCT 
GATTTGGTTG CTGATGAGGA TGACGATGAT AGAGTAAACA CTTCTTTAAA CACATCTGAT 
AACGCATTAT TAGAAGACGA ATTAGAAGAT GACGGGTTTA TACCTGAAAG TGAAGAGGAC 
GGTGATTACA TTGAAAGTGA CTCATCTTTG GATTCAGGCT CGGATTCAGC CAGTGATTCA 
GATGGAGATA ACACCTATCA AGAAGTAGAA GAGGAGGCTG AGGTGAACAC AAATGACAAT 
GAAGATGATT ATATAAGACG ACAAGCGAGT GATGTGGTTC GTACAGATTC AATAATTGAT 
AGAAACGGGC TTCGGAAATC TACAAGAGTC AAGGTGGCGC CTTTGCAGTA TTGGAGGAAC 
GAAAAAATAG TATATAAGAG GAAGTCCAAT AAACCCGTTC TTGACATAGA CAAAATTGTC 
ACATATGATG AATCTGAAGA CGAAGAGGAG ATATTGGCAG CACAAAGAAG GAAGAAACAA 
AAAAAAAAAC CTACACCAAC CAGACCTTAC AACTACGTAC CTACGGGGAG ACCAAGAGGA 
AGGCCAAAGA AGGATCCAAA TGCAAAGGAA AATTTGATTC CAGAAGATCC CAACGAGGAT 
ATTATAGAAA GGATAGAATC TGGAGGCATA GAAAATGGCG AGTGGCTGAA ACATGGAATA 
CTGGAAGCTA ATGTGAAAAT TAGCGACACT AAGGAGGAAA CTAAGGATGA AATTATTGCA 
TTCGCGCCCA ATTTGTCGCA AACTGAACAA GTAAAAGACA CGAAGGACGA GAATTTTGCC 
CTTGAGATAA TGTTCGATAA GCATAAAGAA TATTTTGCTA GCGGCATATT AAAACTACCA 
GCTATTTCTG GACAAAAGAA ATTAAGCAAC TCATTTAGGA CATATATTAC GTTCCACGTG 
ATACAGGGAA TAGTCGAAGT AACTGTATGT AAGAACAAGT TCTTGAGCGT TAAAGGTTCC 
ACTTTCCAAA TACCGGCATT CAACGAGTAC GCGATTGCCA ATAGAGGGAA TGATGAAGCC 
AAAATGTTCT TCGTTCAAGT GACCGTTTCA GAAGACGCTA ACGATGACAA CGACAAAGAA 
TTAGACAGTA CGTTTGACAC TTTTGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012834.1
CDS	1..1650
Translation	MDYMKLGLKSRKTGIDVKQDIPKDEYSMENIDDFFKDDETSLISMRRKSRRKSSLFLPSTLNGDTKNVLPPFLQSYKSQDDEVVQSPSGKGDGSRRSSLLSHQSNFLSPANDFEPIEEEPEQEENDIRGNDFATPITQKLSKPTYKRKYSTRYSLDTSESPSVRLTPDRITNKNVYSDVPDLVADEDDDDRVNTSLNTSDNALLEDELEDDGFIPESEEDGDYIESDSSLDSGSDSASDSDGDNTYQEVEEEAEVNTNDNEDDYIRRQASDVVRTDSIIDRNGLRKSTRVKVAPLQYWRNEKIVYKRKSNKPVLDIDKIVTYDESEDEEEILAAQRRKKQKKKPTPTRPYNYVPTGRPRGRPKKDPNAKENLIPEDPNEDIIERIESGGIENGEWLKHGILEANVKISDTKEETKDEIIAFAPNLSQTEQVKDTKDENFALEIMFDKHKEYFASGILKLPAISGQKKLSNSFRTYITFHVIQGIVEVTVCKNKFLSVKGSTFQIPAFNEYAIANRGNDEAKMFFVQVTVSEDANDDNDKELDSTFDTFG

Target ORF information:

RefSeq Version	NM_001179655.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Mif2p (MIF2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179655.1

ORF Insert Sequence:

ATGGATTATA TGAAATTGGG GCTTAAGTCC CGTAAAACTG GTATTGATGT TAAGCAAGAT 
ATACCCAAAG ATGAATACAG CATGGAAAAT ATTGATGATT TTTTCAAAGA TGATGAAACT 
AGTCTTATCA GTATGAGAAG GAAAAGCAGA AGAAAATCAT CGCTTTTCTT ACCATCAACG 
TTAAATGGCG ATACTAAGAA CGTATTACCG CCATTTCTAC AGTCATATAA ATCTCAAGAT 
GATGAAGTTG TCCAAAGCCC ATCTGGGAAA GGCGATGGAT CAAGACGATC ATCTTTGTTA 
AGCCATCAAT CAAACTTCCT GAGTCCAGCC AATGATTTCG AGCCTATTGA GGAAGAACCA 
GAACAAGAAG AAAATGATAT CAGAGGCAAT GATTTTGCCA CACCAATCAC ACAGAAATTG 
AGTAAACCTA CATATAAAAG AAAGTACTCC ACTCGGTATA GCCTTGACAC TTCAGAAAGC 
CCTTCTGTAA GGTTGACACC TGATAGAATC ACTAATAAGA ATGTTTATTC AGATGTACCT 
GATTTGGTTG CTGATGAGGA TGACGATGAT AGAGTAAACA CTTCTTTAAA CACATCTGAT 
AACGCATTAT TAGAAGACGA ATTAGAAGAT GACGGGTTTA TACCTGAAAG TGAAGAGGAC 
GGTGATTACA TTGAAAGTGA CTCATCTTTG GATTCAGGCT CGGATTCAGC CAGTGATTCA 
GATGGAGATA ACACCTATCA AGAAGTAGAA GAGGAGGCTG AGGTGAACAC AAATGACAAT 
GAAGATGATT ATATAAGACG ACAAGCGAGT GATGTGGTTC GTACAGATTC AATAATTGAT 
AGAAACGGGC TTCGGAAATC TACAAGAGTC AAGGTGGCGC CTTTGCAGTA TTGGAGGAAC 
GAAAAAATAG TATATAAGAG GAAGTCCAAT AAACCCGTTC TTGACATAGA CAAAATTGTC 
ACATATGATG AATCTGAAGA CGAAGAGGAG ATATTGGCAG CACAAAGAAG GAAGAAACAA 
AAAAAAAAAC CTACACCAAC CAGACCTTAC AACTACGTAC CTACGGGGAG ACCAAGAGGA 
AGGCCAAAGA AGGATCCAAA TGCAAAGGAA AATTTGATTC CAGAAGATCC CAACGAGGAT 
ATTATAGAAA GGATAGAATC TGGAGGCATA GAAAATGGCG AGTGGCTGAA ACATGGAATA 
CTGGAAGCTA ATGTGAAAAT TAGCGACACT AAGGAGGAAA CTAAGGATGA AATTATTGCA 
TTCGCGCCCA ATTTGTCGCA AACTGAACAA GTAAAAGACA CGAAGGACGA GAATTTTGCC 
CTTGAGATAA TGTTCGATAA GCATAAAGAA TATTTTGCTA GCGGCATATT AAAACTACCA 
GCTATTTCTG GACAAAAGAA ATTAAGCAAC TCATTTAGGA CATATATTAC GTTCCACGTG 
ATACAGGGAA TAGTCGAAGT AACTGTATGT AAGAACAAGT TCTTGAGCGT TAAAGGTTCC 
ACTTTCCAAA TACCGGCATT CAACGAGTAC GCGATTGCCA ATAGAGGGAA TGATGAAGCC 
AAAATGTTCT TCGTTCAAGT GACCGTTTCA GAAGACGCTA ACGATGACAA CGACAAAGAA 
TTAGACAGTA CGTTTGACAC TTTTGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome XI.
Nature369(6479)371-8(1994 Jun)
Dujon B,Alexandraki D,Andr? B,Ansorge W,Baladron V,Ballesta JP,Banrevi A,Bolle PA,Bolotin-Fukuhara M,Bossier P,Bou G,Boyer J,Bultrago MJ,Cheret G,Colleaux L,Dalgnan-Fornler B,del Rey F,Dlon C,Domdey H,D?sterhoft A,D?sterhus S,Entlan KD,Erfle H,Esteban PF,Feldmann H,Fernandes L,Robo GM,Fritz C,Fukuhara H,Gabel C,Gaillon L,Carcia-Cantalejo JM,Garcia-Ramirez JJ,Gent NE,Ghazvini M,Goffeau A,Gonzal?z A,Grothues D,Guerreiro P,Hegemann J,Hewitt N,Hilger F,Hollenberg CP,Horaitis O,Indge KJ,Jacquier A,James CM,Jauniaux C,Jimenez A,Keuchel H,Kirchrath L,Kleine K,K?tter P,Legrain P,Liebl S,Louis EJ,Maia e Silva A,Marck C,Monnier AL,M?stl D,M?ller S,Obermaier B,Oliver SG,Pallier C,Pascolo S,Pfeiffer F,Philippsen P,Planta RJ,Pohl FM,Pohl TM,P?hlmann R,Portetelle D,Purnelle B,Puzos V,Ramezani Rad M,Rasmussen SW,Remacha M,Revuelta JL,Richard GF,Rieger M,Rodrigues-Pousada C,Rose M,Rupp T,Santos MA,Schwager C,Sensen C,Skala J,Soares H,Sor F,Stegemann J,Tettelin H,Thierry A,Tzermia M,Urrestarazu LA,van Dyck L,Van Vliet-Reedijk JC,Valens M,Vandenbo M,Vilela C,Vissers S,von Wettstein D,Voss H,Wiemann S,Xu G,Zimmermann J,Haasemann M,Becker I,Mewes HW

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MIF2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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