MED8 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MED8
Entrez Gene ID	852492
Full Name	Med8p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178541.3	NP_009752.3	Med8p

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	MED8	NP_009752.3
Eremothecium gossypii	AGOS_AEL252C	NP_984609.1
Kluyveromyces lactis	KLLA0E23607g	XP_455017.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Deletion of med8C, med18, or med20 causes similar changes in the yeast transcriptome.
Title: Structure-system correlation identifies a gene regulatory Mediator submodule.

Our results and published data support a positive role of the Med8/18/20 subcomplex in initiation-complex formation and suggest that the Mediator head contains a multipartite TBP-binding site that can be modulated by transcriptional activators.
Title: Structure and TBP binding of the Mediator head subcomplex Med8-Med18-Med20.

We identified Rgt1 as a transcription factor that, together with the Med8 protein, is essential for repression of the HXK2 gene in the absence of glucose.
Title: Rgt1, a glucose sensing transcription factor, is required for transcriptional repression of the HXK2 gene in Saccharomyces cerevisiae.

The following MED8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01893	NM_001178541.3 Latest version!	Saccharomyces cerevisiae S288C Med8p (MED8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED8 ( NM_001178541.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009752.3 Saccharomyces cerevisiae S288C Med8p (MED8), partial mRNA.

Price & Availability↑

CloneID	OSi01893
Clone ID Related Accession (Same CDS sequence)	NM_001178541.3
Accession Version	NM_001178541.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Med8p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). On Jul 30, 2012 this sequence version replaced NM_001178541.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCACAAT CTACTGCATC ACTGGTACCA GAGGGCAATC AAGGCTCCCT CCAAGAAGAT 
GTTAGTTTTG ATTTCAATGG TGTACCTGGC CAGGCTTTAG ATGCCGTGCG CATGCGTTTG 
GCTCAATTAA CTCATTCATT GAGGAGAATC AGAGACGAAA TGTCCAAAGC CGAACTGCCA 
CAATGGTATA CACTCCAATC ACAACTGAAT GTCACATTAT CCCAGCTCGT TTCTGTAACC 
TCCACTCTAC AACATTTCCA AGAAACTTTA GACTCTACAG TGGTTTACCC TTTGCCTAAA 
TTCCCAACCA CCTCTCATGA AAGTTTGGTC ACTACCCTTT TGAGGAAGAA GAATATTCCT 
GAAGTGGACG AATGGATGAA ATATGTTAGG GAAACATCTG GAGTAACTAC AGCCTTGCTA 
AAGGATGAAG AAATAGAAAA GCTGCTGCAA CAGGACAGAG AAATAACGAA TTGGGCGCGC 
ACTACGTTTA GAAACGAATA TGGAAAGCAC GATTTCAAAA ATGAAGAATC CCTAAGCGAA 
GAACACGCTT CACTTCTAGT ACGAGATAGC AAACCTTCGA AACCTTTCAA TGTGGATGAT 
GTTCTAAAGT TCACCTTCAC AGGGGAAAAG CCCATTATTA CGGGATCTAC TTCAACATCA 
TCTAGTAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009752.3
CDS	1..672
Translation	MSQSTASLVPEGNQGSLQEDVSFDFNGVPGQALDAVRMRLAQLTHSLRRIRDEMSKAELPQWYTLQSQLNVTLSQLVSVTSTLQHFQETLDSTVVYPLPKFPTTSHESLVTTLLRKKNIPEVDEWMKYVRETSGVTTALLKDEEIEKLLQQDREITNWARTTFRNEYGKHDFKNEESLSEEHASLLVRDSKPSKPFNVDDVLKFTFTGEKPIITGSTSTSSSN

Target ORF information:

RefSeq Version	NM_001178541.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Med8p (MED8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178541.3

ORF Insert Sequence:

ATGTCACAAT CTACTGCATC ACTGGTACCA GAGGGCAATC AAGGCTCCCT CCAAGAAGAT 
GTTAGTTTTG ATTTCAATGG TGTACCTGGC CAGGCTTTAG ATGCCGTGCG CATGCGTTTG 
GCTCAATTAA CTCATTCATT GAGGAGAATC AGAGACGAAA TGTCCAAAGC CGAACTGCCA 
CAATGGTATA CACTCCAATC ACAACTGAAT GTCACATTAT CCCAGCTCGT TTCTGTAACC 
TCCACTCTAC AACATTTCCA AGAAACTTTA GACTCTACAG TGGTTTACCC TTTGCCTAAA 
TTCCCAACCA CCTCTCATGA AAGTTTGGTC ACTACCCTTT TGAGGAAGAA GAATATTCCT 
GAAGTGGACG AATGGATGAA ATATGTTAGG GAAACATCTG GAGTAACTAC AGCCTTGCTA 
AAGGATGAAG AAATAGAAAA GCTGCTGCAA CAGGACAGAG AAATAACGAA TTGGGCGCGC 
ACTACGTTTA GAAACGAATA TGGAAAGCAC GATTTCAAAA ATGAAGAATC CCTAAGCGAA 
GAACACGCTT CACTTCTAGT ACGAGATAGC AAACCTTCGA AACCTTTCAA TGTGGATGAT 
GTTCTAAAGT TCACCTTCAC AGGGGAAAAG CCCATTATTA CGGGATCTAC TTCAACATCA 
TCTAGTAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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MED8 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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