IRC7 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	IRC7
Entrez Gene ID	850616
Full Name	cysteine-S-conjugate beta-lyase IRC7
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180021.1	NP_116714.1	cysteine-S-conjugate beta-lyase IRC7

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Murakami Y, Naitou M, Hagiwara H, Shibata T, Ozawa M, Sasanuma S, Sasanuma M, Tsuchiya Y, Soeda E, Yokoyama K
Nature genetics10(3)261-8(1995 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

While IRC7 has been identified as playing a critical role in converting cysteine conjugates to volatile thiols that are important in wine aroma, its biological role in yeast cells is likely to involve regulation of cysteine and redox homeostasis.
Title: The IRC7 gene encodes cysteine desulphydrase activity and confers on yeast the ability to grow on cysteine as a nitrogen source.

IRC7 gene encodes a beta-lyase responsible for production of the varietal thiol 4-mercapto-4-methylpentan-2-one in wine.
Title: The yeast IRC7 gene encodes a ?-lyase responsible for production of the varietal thiol 4-mercapto-4-methylpentan-2-one in wine.

The following IRC7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IRC7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00292	NM_001180021.1 Latest version!	Saccharomyces cerevisiae S288C cysteine-S-conjugate beta-lyase IRC7 (IRC7), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IRC7 ( NM_001180021.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_116714.1 Saccharomyces cerevisiae S288C cysteine-S-conjugate beta-lyase IRC7 (IRC7), partial mRNA.

Price & Availability↑

CloneID	OSi00292
Clone ID Related Accession (Same CDS sequence)	NM_001180021.1
Accession Version	NM_001180021.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	cysteine-S-conjugate beta-lyase IRC7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001138). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGATTGATC GTACCGAGTT ATCGAAGTTT GGTATTACTA CGCAACTGTC TGTTATTGGA 
CGTAATCCAG ATGAACAAAG TGGCTTTGTT AATCCACCTT TGTATAAGGG GTCAACCATC 
ATTCTTAAAA AACTTAGTGA TTTAGAACAA AGGAAAGGAA GATTTTACGG GACAGCAGGT 
TCTCCAACTA TTGACAATTT AGAAAATGCC TGGACGCATT TAACCGGCGG TGCTGGGACA 
GTGCTATCAG CTTCTGGGCT TGGTTCTATC TCTTTGGCGC TATTGGCCCT TTCGAAAGCT 
GGTGATCATA TCTTGATGAC TGATAGTGTC TACGTGCCAA CACGTATGCT ATGTGATGGT 
TTATTGGCCA AGTTCGGTGT TGAAACGGAT TATTATGACC CATCAATAGG GAAGGATATA 
GAAAAACTAG TTAAGCCAAA TACAACCGTC ATTTTCCTCG AAAGCCCGGG TTCTGGGACC 
ATGGAAGTAC AGGATATTCC AGCTTTGGTC TCTGTTGCCA AAAAGCATGG GATAAAGACA 
ATTCTAGACA ACACATGGGC AACGCCACTC TTTTTTGATG CTCATGCGCA TGGTATCGAT 
ATTTCGGTAG AAGCTGGGAC AAAATATTTG GGTGGTCATT CAGATCTTCT TATTGGTCTG 
GCCTCCGCAA ATGAAGAATG TTGGCCGCTA TTACGGTCAA CTTATGATGC AATGGCAATG 
TTACCAGGTG CCGAGGACTG TCAATTAGCA TTGCGAGGAA TGCGTACATT GCACTTAAGA 
TTGAAAGAGG TAGAAAGAAA AGCCCTGGAT TTGGCTGCTT GGCTCGGAAA TCGAGATGAG 
GTTGAAAAAG TGCTTCACCC CGCCTTTGAA GATTGTCCCG GACATGAATA CTGGGTTCGT 
GACTACAAAG GTTCTTCAGG CTTATTTTCC ATTGTCCTTA AAAATGGGTT CACAAGAGCT 
GGTCTGGAGA AAATGGTAGA AGGGATGAAA GTTTTGCAAT TGGGATTTTC ATGGGGTGGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116714.1
CDS	1..1023
Translation	MIDRTELSKFGITTQLSVIGRNPDEQSGFVNPPLYKGSTIILKKLSDLEQRKGRFYGTAGSPTIDNLENAWTHLTGGAGTVLSASGLGSISLALLALSKAGDHILMTDSVYVPTRMLCDGLLAKFGVETDYYDPSIGKDIEKLVKPNTTVIFLESPGSGTMEVQDIPALVSVAKKHGIKTILDNTWATPLFFDAHAHGIDISVEAGTKYLGGHSDLLIGLASANEECWPLLRSTYDAMAMLPGAEDCQLALRGMRTLHLRLKEVERKALDLAAWLGNRDEVEKVLHPAFEDCPGHEYWVRDYKGSSGLFSIVLKNGFTRAGLEKMVEGMKVLQLGFSWGG

Target ORF information:

RefSeq Version	NM_001180021.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C cysteine-S-conjugate beta-lyase IRC7 (IRC7), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180021.1

ORF Insert Sequence:

ATGATTGATC GTACCGAGTT ATCGAAGTTT GGTATTACTA CGCAACTGTC TGTTATTGGA 
CGTAATCCAG ATGAACAAAG TGGCTTTGTT AATCCACCTT TGTATAAGGG GTCAACCATC 
ATTCTTAAAA AACTTAGTGA TTTAGAACAA AGGAAAGGAA GATTTTACGG GACAGCAGGT 
TCTCCAACTA TTGACAATTT AGAAAATGCC TGGACGCATT TAACCGGCGG TGCTGGGACA 
GTGCTATCAG CTTCTGGGCT TGGTTCTATC TCTTTGGCGC TATTGGCCCT TTCGAAAGCT 
GGTGATCATA TCTTGATGAC TGATAGTGTC TACGTGCCAA CACGTATGCT ATGTGATGGT 
TTATTGGCCA AGTTCGGTGT TGAAACGGAT TATTATGACC CATCAATAGG GAAGGATATA 
GAAAAACTAG TTAAGCCAAA TACAACCGTC ATTTTCCTCG AAAGCCCGGG TTCTGGGACC 
ATGGAAGTAC AGGATATTCC AGCTTTGGTC TCTGTTGCCA AAAAGCATGG GATAAAGACA 
ATTCTAGACA ACACATGGGC AACGCCACTC TTTTTTGATG CTCATGCGCA TGGTATCGAT 
ATTTCGGTAG AAGCTGGGAC AAAATATTTG GGTGGTCATT CAGATCTTCT TATTGGTCTG 
GCCTCCGCAA ATGAAGAATG TTGGCCGCTA TTACGGTCAA CTTATGATGC AATGGCAATG 
TTACCAGGTG CCGAGGACTG TCAATTAGCA TTGCGAGGAA TGCGTACATT GCACTTAAGA 
TTGAAAGAGG TAGAAAGAAA AGCCCTGGAT TTGGCTGCTT GGCTCGGAAA TCGAGATGAG 
GTTGAAAAAG TGCTTCACCC CGCCTTTGAA GATTGTCCCG GACATGAATA CTGGGTTCGT 
GACTACAAAG GTTCTTCAGG CTTATTTTCC ATTGTCCTTA AAAATGGGTT CACAAGAGCT 
GGTCTGGAGA AAATGGTAGA AGGGATGAAA GTTTTGCAAT TGGGATTTTC ATGGGGTGGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Nature genetics10(3)261-8(1995 Jul)
Murakami Y,Naitou M,Hagiwara H,Shibata T,Ozawa M,Sasanuma S,Sasanuma M,Tsuchiya Y,Soeda E,Yokoyama K

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IRC7 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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