PHO4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Murakami Y, Naitou M, Hagiwara H, Shibata T, Ozawa M, Sasanuma S, Sasanuma M, Tsuchiya Y, Soeda E, Yokoyama K
Nature genetics10(3)261-8(1995 Jul)

Pho85 and Pho4 regulated CLN2 expression under alkaline condition.
Title: The regulators of yeast PHO system participate in the transcriptional regulation of G1 cyclin under alkaline stress conditions.

Subsequent in-depth analysis of the inducible PHO5 gene reveals that topoisomerases are essential for binding of the Pho4p transcription factor to the PHO5 promoter, which is required for promoter nucleosome removal during activation.
Title: DNA Topoisomerases maintain promoters in a state competent for transcriptional activation in Saccharomyces cerevisiae.

The authors postulate that RPL12 has an extra-ribosomal function in modulating the transcription of genes that need Pho4p activation.
Title: Rpl12p affects the transcription of the PHO pathway high-affinity inorganic phosphate transporters and repressible phosphatases.

Pho4 activates some stress response genes, is required for G1 arrest caused by DNA damage, and is present in the nucleus even under high-Pi conditions to activate or repress transcription.
Title: Nutrient-regulated antisense and intragenic RNAs modulate a signal transduction pathway in yeast.

Pho4 binding to the SNZ1/SNO1 promoter is accompanied by alterations in chromatin structure, and Rpd3 histone deacetylase is required for the proper timing of SNZ1/SNO1 expression, while Asf1 histone chaperone is indispensable for their expression.
Title: Transcriptional repression by the Pho4 transcription factor controls the timing of SNZ1 expression.