MRC1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MRC1
Entrez Gene ID	850297
Full Name	chromatin-modulating protein MRC1
Synonyms	YCL060C
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178704.1	NP_009871.2	chromatin-modulating protein MRC1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	MRC1	NP_009871.2
Eremothecium gossypii	AGOS_AFR745W	NP_986294.1
Kluyveromyces lactis	KLLA0C00484g	XP_452215.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

The complete DNA sequence of yeast chromosome III.
Oliver SG, van der Aart QJ, Agostoni-Carbone ML, Aigle M, Alberghina L, Alexandraki D, Antoine G, Anwar R, Ballesta JP, Benit P
Nature357(6373)38-46(1992 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dia2-dependent degradation of Mrc1 is required for replication fork restart in a pathway that includes Sgs1 and Mph1.
Title: Degradation of Mrc1 promotes recombination-mediated restart of stalled replication forks.

Authors show that minisatellite length expansions are controlled by the products of the CSM3 and TOF1 genes, while contractions are controlled by MRC1.
Title: The role of CSM3, MRC1, and TOF1 in minisatellite stability and large loop DNA repair during meiosis in yeast.

Whereas Ddc1 and Dpb11 aid in replication-checkpoint activation, colocalization of Mec1 and Mrc1 at stalled replication forks promotes Rad53 activation sufficient to stabilize the replisome during transient replication stress.
Title: Colocalization of Mec1 and Mrc1 is sufficient for Rad53 phosphorylation in vivo.

Data show that that ISC1 controls cell morphology in combination with checkpoint regulators Swe1, Cdk1(CDC28), MRC1, TOF1, CSM3, RAD9 and Rad53.
Title: Cellular morphogenesis under stress is influenced by the sphingolipid pathway gene ISC1 and DNA integrity checkpoint genes in Saccharomyces cerevisiae.

cells deleted of MRC1, a gene implicated in replication fork stabilization and in the replication checkpoint pathway, maintained wild-type firing times despite over twofold lengthening of S phase
Title: MRC1-dependent scaling of the budding yeast DNA replication timing program.

The following MRC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MRC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00026	NM_001178704.1 Latest version!	Saccharomyces cerevisiae S288C chromatin-modulating protein MRC1 (MRC1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MRC1 ( NM_001178704.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009871.2 Saccharomyces cerevisiae S288C chromatin-modulating protein MRC1 (MRC1), partial mRNA.

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CloneID	OSi00026
Clone ID Related Accession (Same CDS sequence)	NM_001178704.1
Accession Version	NM_001178704.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	chromatin-modulating protein MRC1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001135). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGATGATG CCTTGCATGC TTTGTCCTCG TTGACTGCAA AGAAGAGAAC TACCACCTAC 
AAAAAAGTTG CTGTTCCCAT ACTAGATGAG AACGATAATA CTAATGGAAA TGGGCCCAAT 
GACATAGACA ATCCGCCAGA GTTGACTGGG AACGGGTTTT TATTTGCCAA TGCCACATTA 
AATCGTGTTA AGAACAGATT AGAAGGCAAG AAAGCACCCG AACAGAATCA CAATAATGGA 
AAAGATAGGA GTGAGAATTC GTTACCAACT CAATTAATCT CTAATCTTTA CGATGGTGGT 
GAAGAGCTGG AGAAATCCGA AGTTAAAGAT AATAGTTACA GTGAGAAAAA TGTCTCTTCT 
TCATTCACGC AGACGCAAAG AATACCTGTC AGCATCCAAC AAGACAAGGT GTTTAACGTA 
CCTATCCATT CCGTTAATGA CGGGAAACCC ACCCAATTAA TCAAAGAAGA TGGTCTCGTC 
AACGAAACTT CTCAAGCGCT GAAAACACCT CTCACGACAG GACGGCCAGG GGCTACACAG 
CGTATAGATA GCAGCGGTGC AACCTCACAA ACTCAGCCTA TAAAATCAAT TGAACCACAA 
TCCCAAATAA TTACGACTTC CAGCAATCAT TCTAATGCTC TGTCGCCGAA AATTCCTATA 
ATTCCAACGG AACTTATTGG AACTAGTCCT TTATTTCAGT CCATTCAAAA TCGTGGTCCT 
GACACCCAAA TGGATGTACC ACCACAGACT GCGCATGATG AGGATAAGAC ACAAGCAATT 
GGTATTCCAC AGGCTACGCA TCAGGAACAG AAAACCCAAA TAGATACAGT CGCACAAACA 
CTTCAAGATG AAGTACCACA CACATTAAAA ATTCGGGAAA TACAAAGCGA ATTGGCTTCA 
GAAGATTCTA AAAGGGAAAA AGCTAGAAAT GTCGAATACA AAAAACCGCA AAAGCCAATA 
CCAACCAAAA AATTTTTTTC TAAAGAGTCT TTTCTTGCTG ATTTTGACGA TTCGTCCTCA 
AATGAGGACG ATGATATAAA GCTGGAAAAT GCACATCCAA AACCAGTACA GAATGACGAT 
GAACTGCATG AGAACAAAAG TGTTGAATTA AATCTAACTG ATGAGACAAG AATAAATGAG 
AAAAGGGTTC CACTATTGAG CTCCTATGCT AATAATTTGA AACGGGAAAT TGACTCCTCG 
AAATGCATAA CACTGGACCT CGACAGCGAC AGTGACGAAT ATGGAGATGA TGATATGGAT 
AGCATTAAAT TATCCAAAGA TGAAAGCGTT TTGCCAATCT CTCAGTTATC AAAGGCGACT 
ATATTAAACC TGAAGGCAAG ACTCTCTAAA CAAAATCAAA AGTTGTCTCA AAGGCCGAAT 
AAAAGTAAAG ACCCAAAAGT TGATCATAAT GTACTATTAA ATACCTTAAG GAAAGCTAGC 
AGGAAACAAA TTCTGGATCA TCAGAAAGAA GTGATTGAAA CGAAAGGTCT GAAGCTGGAG 
GATATGGCCA AAGAAAAGGA AATAGTAGAG AACTTGCTAG AACAAGAAAT TCTTAGAAAT 
AAAAGAATCA GACAAAAAGA GAAGAGAAGG GAAAAACTTG AAGAAAATGA CTTTCAATTG 
AACGCCCATG ATTCCGGTTC TGACTCAGGG TCAGAGTCTT CTGGATTTGC ATTGAGTGGT 
AATGAAATTG CCGATTATGA ATCATCTGGT AGTGAAAATG ACAATCGTAG AGAATCCGAT 
AGTGAAAAGG AAGATGATGA AATTATTCTA AAGCAAAAAA AATCCCACCA CGTGAAACAT 
ATCATTAACG AATCAGACTC TGATACCGAA GTCGAAGCCA AACCCAAAGA GAAAGCTGAT 
GAAAGTCTAC CGAAAAGGAT CGCCATTAAT CTGGGCCATT ACGGTGATAA TATCGGAGAG 
GATACCGACA AATTTCAAGA AACCAATGTT CTAGATACAC AAAACATTGA AGAGGTGATG 
GCCGAAAGAA ATACAATCGA AAATGAAGTC AAAGATGATG TATACGTCAA TGAAGAAGCT 
GATGAAGCAA TTCGCCGTCA GCTGATTGAC AAAGAGAAAT TGCAACTAAA ACAGAAAGAA 
AAGGAGCACG AGGCTAAGAT AAAAGAGTTG AAGAAAAGAG GTGTCACAAA CTTCTTTGAA 
ATGGAAGCCG AAGAATCCGA GGATGAATGG CATGGTATCG GTGGTGCAGA TGGAGAAGGA 
TCTGACGACT ATGATTCTGA CCTAGAAAAA ATGATCGATG ATTATTCCAA GAACAATTTC 
AATCCTCATG AAATCAGAGA AATGCTTGCC GCAGAAAATA AGGAAATGGA TATCAAAATG 
ATAAACAAAA TTCTTTATGA CATTAAAAAT GGAGGATTTC GTAATAAAAG AGCAAAAAAT 
AGCTTAGAGC TAGAACTAAG TGATGATGAT GAAGACGACG TACTACAACA ATATCGTCTC 
AAAAGGAGAG AATTGATGAG AAAAAGGAGA TTAGAAATTG GCGATGATGC AAAGCTTGTT 
AAAAACCCAA AGTCGAGCGC ATTTTTTGAA AGTATGGTGG AAGATATTAT CGAATATAAA 
AACCCCTTCG GGGCTGAAGA GGAATATAAC CTAGACATTA CAAGTACGGC TACTGACCTA 
GATACTCAGG ACAATAGCAT TAATGTAGGT GATAATACGG GAAATAATGA ACAGAAACCT 
GTAGATCAAA AGAACAAAAA GGTTATCATA TCTGAAGACT TTGTGCAGAA AAGCTTATCC 
TTCCTAAAAA GTAATAATTA TGAGGATTTT GAAACGGATA AGGAGCTATC AAGGATACAA 
CATGGCAATG ATGAAGCAAT TGAAGATTTA TATACCCTAA AACAGAATAG TTCGATAAAG 
TCATTCACAA ATTCTCAAAC TGATTCGACC ACTTCAAAAA CAGTAAACAC AATAATTGAC 
TTAGAAAAGC GCCCAGAGGA TGAAGATGAG GTGGAAAATG GAGATACATC ACTAGTTGGC 
GTTTTCAAAC ATCCATCAAT AATCAAATCC TTTGCGTCCA GAACAGACAT AAATGATAAG 
TTTAAAGAAG GTAACAAGAC AGTCAAGATT CTAAAGTCTT ACAAGACAGT GGGGAGTTCA 
AAAGCTTCTA TCACATATAT GGGGAAAACG AGAAAATTAA TAGCACCAAA GAGGAAAACT 
GAAGGGAGCC ACCGCTACCA CCATGACCAT CATAATAAAA AAATGAAAAT GAAAACGAAA 
ACAAAATCGA ATAAACTTTT TGAAAGCGGA CAAGATAGCT TTGATAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009871.2
CDS	1..3291
Translation	MDDALHALSSLTAKKRTTTYKKVAVPILDENDNTNGNGPNDIDNPPELTGNGFLFANATLNRVKNRLEGKKAPEQNHNNGKDRSENSLPTQLISNLYDGGEELEKSEVKDNSYSEKNVSSSFTQTQRIPVSIQQDKVFNVPIHSVNDGKPTQLIKEDGLVNETSQALKTPLTTGRPGATQRIDSSGATSQTQPIKSIEPQSQIITTSSNHSNALSPKIPIIPTELIGTSPLFQSIQNRGPDTQMDVPPQTAHDEDKTQAIGIPQATHQEQKTQIDTVAQTLQDEVPHTLKIREIQSELASEDSKREKARNVEYKKPQKPIPTKKFFSKESFLADFDDSSSNEDDDIKLENAHPKPVQNDDELHENKSVELNLTDETRINEKRVPLLSSYANNLKREIDSSKCITLDLDSDSDEYGDDDMDSIKLSKDESVLPISQLSKATILNLKARLSKQNQKLSQRPNKSKDPKVDHNVLLNTLRKASRKQILDHQKEVIETKGLKLEDMAKEKEIVENLLEQEILRNKRIRQKEKRREKLEENDFQLNAHDSGSDSGSESSGFALSGNEIADYESSGSENDNRRESDSEKEDDEIILKQKKSHHVKHIINESDSDTEVEAKPKEKADESLPKRIAINLGHYGDNIGEDTDKFQETNVLDTQNIEEVMAERNTIENEVKDDVYVNEEADEAIRRQLIDKEKLQLKQKEKEHEAKIKELKKRGVTNFFEMEAEESEDEWHGIGGADGEGSDDYDSDLEKMIDDYSKNNFNPHEIREMLAAENKEMDIKMINKILYDIKNGGFRNKRAKNSLELELSDDDEDDVLQQYRLKRRELMRKRRLEIGDDAKLVKNPKSSAFFESMVEDIIEYKNPFGAEEEYNLDITSTATDLDTQDNSINVGDNTGNNEQKPVDQKNKKVIISEDFVQKSLSFLKSNNYEDFETDKELSRIQHGNDEAIEDLYTLKQNSSIKSFTNSQTDSTTSKTVNTIIDLEKRPEDEDEVENGDTSLVGVFKHPSIIKSFASRTDINDKFKEGNKTVKILKSYKTVGSSKASITYMGKTRKLIAPKRKTEGSHRYHHDHHNKKMKMKTKTKSNKLFESGQDSFDN

Target ORF information:

RefSeq Version	NM_001178704.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C chromatin-modulating protein MRC1 (MRC1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178704.1

ORF Insert Sequence:

ATGGATGATG CCTTGCATGC TTTGTCCTCG TTGACTGCAA AGAAGAGAAC TACCACCTAC 
AAAAAAGTTG CTGTTCCCAT ACTAGATGAG AACGATAATA CTAATGGAAA TGGGCCCAAT 
GACATAGACA ATCCGCCAGA GTTGACTGGG AACGGGTTTT TATTTGCCAA TGCCACATTA 
AATCGTGTTA AGAACAGATT AGAAGGCAAG AAAGCACCCG AACAGAATCA CAATAATGGA 
AAAGATAGGA GTGAGAATTC GTTACCAACT CAATTAATCT CTAATCTTTA CGATGGTGGT 
GAAGAGCTGG AGAAATCCGA AGTTAAAGAT AATAGTTACA GTGAGAAAAA TGTCTCTTCT 
TCATTCACGC AGACGCAAAG AATACCTGTC AGCATCCAAC AAGACAAGGT GTTTAACGTA 
CCTATCCATT CCGTTAATGA CGGGAAACCC ACCCAATTAA TCAAAGAAGA TGGTCTCGTC 
AACGAAACTT CTCAAGCGCT GAAAACACCT CTCACGACAG GACGGCCAGG GGCTACACAG 
CGTATAGATA GCAGCGGTGC AACCTCACAA ACTCAGCCTA TAAAATCAAT TGAACCACAA 
TCCCAAATAA TTACGACTTC CAGCAATCAT TCTAATGCTC TGTCGCCGAA AATTCCTATA 
ATTCCAACGG AACTTATTGG AACTAGTCCT TTATTTCAGT CCATTCAAAA TCGTGGTCCT 
GACACCCAAA TGGATGTACC ACCACAGACT GCGCATGATG AGGATAAGAC ACAAGCAATT 
GGTATTCCAC AGGCTACGCA TCAGGAACAG AAAACCCAAA TAGATACAGT CGCACAAACA 
CTTCAAGATG AAGTACCACA CACATTAAAA ATTCGGGAAA TACAAAGCGA ATTGGCTTCA 
GAAGATTCTA AAAGGGAAAA AGCTAGAAAT GTCGAATACA AAAAACCGCA AAAGCCAATA 
CCAACCAAAA AATTTTTTTC TAAAGAGTCT TTTCTTGCTG ATTTTGACGA TTCGTCCTCA 
AATGAGGACG ATGATATAAA GCTGGAAAAT GCACATCCAA AACCAGTACA GAATGACGAT 
GAACTGCATG AGAACAAAAG TGTTGAATTA AATCTAACTG ATGAGACAAG AATAAATGAG 
AAAAGGGTTC CACTATTGAG CTCCTATGCT AATAATTTGA AACGGGAAAT TGACTCCTCG 
AAATGCATAA CACTGGACCT CGACAGCGAC AGTGACGAAT ATGGAGATGA TGATATGGAT 
AGCATTAAAT TATCCAAAGA TGAAAGCGTT TTGCCAATCT CTCAGTTATC AAAGGCGACT 
ATATTAAACC TGAAGGCAAG ACTCTCTAAA CAAAATCAAA AGTTGTCTCA AAGGCCGAAT 
AAAAGTAAAG ACCCAAAAGT TGATCATAAT GTACTATTAA ATACCTTAAG GAAAGCTAGC 
AGGAAACAAA TTCTGGATCA TCAGAAAGAA GTGATTGAAA CGAAAGGTCT GAAGCTGGAG 
GATATGGCCA AAGAAAAGGA AATAGTAGAG AACTTGCTAG AACAAGAAAT TCTTAGAAAT 
AAAAGAATCA GACAAAAAGA GAAGAGAAGG GAAAAACTTG AAGAAAATGA CTTTCAATTG 
AACGCCCATG ATTCCGGTTC TGACTCAGGG TCAGAGTCTT CTGGATTTGC ATTGAGTGGT 
AATGAAATTG CCGATTATGA ATCATCTGGT AGTGAAAATG ACAATCGTAG AGAATCCGAT 
AGTGAAAAGG AAGATGATGA AATTATTCTA AAGCAAAAAA AATCCCACCA CGTGAAACAT 
ATCATTAACG AATCAGACTC TGATACCGAA GTCGAAGCCA AACCCAAAGA GAAAGCTGAT 
GAAAGTCTAC CGAAAAGGAT CGCCATTAAT CTGGGCCATT ACGGTGATAA TATCGGAGAG 
GATACCGACA AATTTCAAGA AACCAATGTT CTAGATACAC AAAACATTGA AGAGGTGATG 
GCCGAAAGAA ATACAATCGA AAATGAAGTC AAAGATGATG TATACGTCAA TGAAGAAGCT 
GATGAAGCAA TTCGCCGTCA GCTGATTGAC AAAGAGAAAT TGCAACTAAA ACAGAAAGAA 
AAGGAGCACG AGGCTAAGAT AAAAGAGTTG AAGAAAAGAG GTGTCACAAA CTTCTTTGAA 
ATGGAAGCCG AAGAATCCGA GGATGAATGG CATGGTATCG GTGGTGCAGA TGGAGAAGGA 
TCTGACGACT ATGATTCTGA CCTAGAAAAA ATGATCGATG ATTATTCCAA GAACAATTTC 
AATCCTCATG AAATCAGAGA AATGCTTGCC GCAGAAAATA AGGAAATGGA TATCAAAATG 
ATAAACAAAA TTCTTTATGA CATTAAAAAT GGAGGATTTC GTAATAAAAG AGCAAAAAAT 
AGCTTAGAGC TAGAACTAAG TGATGATGAT GAAGACGACG TACTACAACA ATATCGTCTC 
AAAAGGAGAG AATTGATGAG AAAAAGGAGA TTAGAAATTG GCGATGATGC AAAGCTTGTT 
AAAAACCCAA AGTCGAGCGC ATTTTTTGAA AGTATGGTGG AAGATATTAT CGAATATAAA 
AACCCCTTCG GGGCTGAAGA GGAATATAAC CTAGACATTA CAAGTACGGC TACTGACCTA 
GATACTCAGG ACAATAGCAT TAATGTAGGT GATAATACGG GAAATAATGA ACAGAAACCT 
GTAGATCAAA AGAACAAAAA GGTTATCATA TCTGAAGACT TTGTGCAGAA AAGCTTATCC 
TTCCTAAAAA GTAATAATTA TGAGGATTTT GAAACGGATA AGGAGCTATC AAGGATACAA 
CATGGCAATG ATGAAGCAAT TGAAGATTTA TATACCCTAA AACAGAATAG TTCGATAAAG 
TCATTCACAA ATTCTCAAAC TGATTCGACC ACTTCAAAAA CAGTAAACAC AATAATTGAC 
TTAGAAAAGC GCCCAGAGGA TGAAGATGAG GTGGAAAATG GAGATACATC ACTAGTTGGC 
GTTTTCAAAC ATCCATCAAT AATCAAATCC TTTGCGTCCA GAACAGACAT AAATGATAAG 
TTTAAAGAAG GTAACAAGAC AGTCAAGATT CTAAAGTCTT ACAAGACAGT GGGGAGTTCA 
AAAGCTTCTA TCACATATAT GGGGAAAACG AGAAAATTAA TAGCACCAAA GAGGAAAACT 
GAAGGGAGCC ACCGCTACCA CCATGACCAT CATAATAAAA AAATGAAAAT GAAAACGAAA 
ACAAAATCGA ATAAACTTTT TGAAAGCGGA CAAGATAGCT TTGATAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

The complete DNA sequence of yeast chromosome III.
Nature357(6373)38-46(1992 May)
Oliver SG,van der Aart QJ,Agostoni-Carbone ML,Aigle M,Alberghina L,Alexandraki D,Antoine G,Anwar R,Ballesta JP,Benit P

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MRC1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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