tif471 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	tif471
Entrez Gene ID	2542290
Full Name	translation initiation factor eIF4G
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001020030.2	NP_594602.1	translation initiation factor eIF4G

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tif471 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tif471 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc103948	NM_001020030.2 Latest version!	Schizosaccharomyces pombe translation initiation factor eIF4G (tif471), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tif471 ( NM_001020030.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_594602.1 Schizosaccharomyces pombe translation initiation factor eIF4G (tif471), mRNA.

Price & Availability↑

CloneID	OSc103948
Clone ID Related Accession (Same CDS sequence)	NM_001020030.2
Accession Version	NM_001020030.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	translation initiation factor eIF4G
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001020030.1.

ATGTCTTCAA AACCACCGTC AAATACTCCA AAGTTTAGTT ATGCTCGTGC CTTGGCCAGC 
AGTCAATCCA ATAAAAGCAA TAGCACTAAA GCTTCAGAAA ATAATACTGC AACTGCTGAA 
AAGCAGGCAG TAAAGCCTTC AGGGGTGGAG CCAACGAATA CCTCTCGTGC TAATGCCCAA 
AAGAAAACAG AGTCCACTGG AAAGATTACT TCAGAGGCTG ACACTGAAAA GTACAACTCA 
AGCAAATCTC CTGTCAACAA GGAAGGATCG GTGGAAAAAA AATCTTCTGA AAAATCTTCA 
ACCAATAATA AGCCATGGCG CGGCGATAAT ACTTCAAAAC CATCGGCCAA TAGCTCTGCT 
GAACGAACGA GCTCACAGCA TCAGAAGCCT GAAACTTCAT CCCAAATTGG TAAAGACAAT 
GCAGCTCCCG TGGAAAATGT AAATGAGAAA TCTACCTCCC AAGAAACAGC ACCTCCTGTA 
TCCACTGTGC CAATTCAGTT TGGTTCGATT ACCAGAAATG CGGCTATCCC TTCAAAACCA 
AAGGTTTCTG GTAACATGCA AAATAAGAGT GGCGTGTCTT CTTATAGTTC CAAAAGCCAG 
TCTGTTAACT CCTCGGTTAC ATCCAATCCT CCTCATACAG AAGAACCTGT TGCTGCTAAG 
CCGGAGGCTT CTTCAACCGC CACTAAAGGA CCTCGTCCTA CCACGAGTGC ATCCAACACC 
AATACCTCTC CTGCTAATGG CGCACCGACT AATAAACCTT CAACCGATAT CAATACCACT 
GATCCCGCCA CCCAAACTAC CCAAGTTTCA GCGTCCAACT CTCCTGCATT GTCAGGAAGT 
AGTACACCAT CAAACACATC TTCTAGATCT AATCGTCAAA ATCACGGAAA CTTTTCCGAA 
AAGCGGCACT ACGACCGTTA CGGAAATTCT CATCCTTCTT ACAATAAATA TTCTCATTAT 
CAGCATGGTT TTAATTATAA TAACTCGGGA AATAATAGGA ATGAGTCAGG GCATCCTCGT 
TTTCGAAACT CTAGGAGAAA CTATAACAAT CAAGGTGCCT ATCCTACGTA TATGTCTAAT 
GGACGTAGTG CCAATCAAAG CCCTCGGAAC AATCCCCAAA ACGTAAACAA TGGTAGTACA 
CCAATTCAAA TTCCAGTTTC TTTACAGACT CCTTACGGAC AAGTATATGG GCAACCACAA 
TACATCGTGG ATCCCAATAT GGTGCAGTAT GGTCCTATCC TTCAACCTGG TTATGTTCCT 
CAATATTACC CAGTTTACCA TCAGACACCC TATACTCAGA ACTTTCCTAA TATGTCAAGA 
AGCGGCTCTC AAGTTTCTGA TCAAGTTGTT GAATCTCCTA ATTCATCTAC ACTTAGCCCC 
AGAAATGGAT TTGCCCCTAT AGTGAAGCAA CAGAAAAAGA GTAGCGCATT GAAGATTGTC 
AATCCCGTAA CACATACAGA AGTAGTGGTT CCGCAAAAAA ATGCGTCGTC TCCTAATCCC 
TCTGAAACTA ACTCTCGTGC TGAAACACCA ACTGCTGCTC CTCCTCAGAT TTCAGAAGAG 
GAAGCTTCAC AGCGAAAAGA TGCTATTAAG CTTGCCATTC AACAGCGTAT CCAGGAAAAA 
GCGGAAGCTG AAGCAAAGCG TAAAGCCGAA GAGAAGGCCA GACTAGAAGC GGAAGAAAAT 
GCCAAACGTG AAGCGGAAGA ACAAGCAAAG CGTGAAGCGG AAGAAAAGGC TAAACGTGAA 
GCTGAAGAGA AGGCCAAACG TGAAGCTGAA GAGAAGGCCA AACGTGAAGC TGAAGAGAAT 
GCCAAACGTG AAGCTGAGGA GAAGGCTAAG CGCGAAGCTG AAGAGAAGGC TAAACGTGAA 
GCTGAAGAAA AAGCCAAACG TGAAGCTGAA GAAAAGGCTA AGCGTGAAGC TGAAGAAAAG 
GCTAAACGCG AAGCTGAAGA AAAGGCTAAG CGTGAAGCTG AAGAAAAGGC TAAGCGCGAA 
GCTGAAGAGA ATGCTAAACG TGAAGCTGAG GAGAAGGCTA AACGTGAAGC TGAAGAAAAC 
GCTAAACGTG AAGCTGAAGA GAAGGTTAAA CGTGAGACTG AGGAAAATGC TAAACGGAAA 
GCTGAGGAAG AGGGTAAACG GGAAGCTGAT AAAAACCCTG AAATCAAATC AAGTGCACCC 
CTTGCTTCGA GTGAAGCTAA CGTTGATACC AGTAAGCAAA CAAATGCTAC CGAACCTGAA 
GTGGTTGACA AAACAAAAGT GGAGAAGCTT AAGGCATCTG AAGGAAAATC GACTTCTTCG 
CTTTCTTCTC CCTCTCATTC GACCTCTTCT AAACGGGACT TGCTATCTGG TCTGGAAAGC 
CTCAGTCTGA AAACTAACCC CAAATCTGAG CAGTGCTTAG AATCATTGTT AAATTCTCAA 
TTTATAACCG ACTTTTCTGC TTTGGTTTAT CCTTCCACTA TCAAACCACC ATCTACTGAG 
GAAGCCTTAA AAGCTGGTAA ATATGAGTAT GATGTTCCTT TCCTTTTACA GTTTCAAAGC 
GTTTATACTG ATAAGCCAAT GAAAGGTTGG GACGAAAGGA TGAAGGAAAC AGTAGCATCC 
GCTTTTAGTG ATAAAAGCTC TCGTGGAATG TACAGCTCTA GTCGCCAAAG CTCTCGATCC 
GGCTCAAATA CTCACTCCCA TGCTGGCCCT GGTTTTGGTG GTCCTTCAGA GAGAAAGGGC 
ATTTCAAGGC TTGGAATCGA TCGAGGTTTC AGCTCCTCTG GTGCTGGATT TGGATCGGGT 
AGTAATTATA AGAGCGCTCC TAGTCGCGGT GTAAGTCATC ACGGACACGG TGGTATGTCT 
GGGTCTCATC GTGGTTCCCA GCGTGGTTCT CGCCGCGGAG GTGGTGAGCG TGATAAACCC 
GATCCATCTA GTCTAACTAT ACCTGTTGAT CAGGTAGCAC CTCTTCAATT ATCCGCAAAC 
CGCTGGCAAC CTAAGAAACT TACTGAAAAA CCTGCTGAAA CTAAGGGTGA GGATGAAGAA 
GCACTTCTTC CTCCCGAGGT CGTGCAGCGT AAGGTTAAAG GTTCTTTGAA CAAGATGACA 
TTAGAGAAGT TTGATAAAAT ATCTGACCAG ATTTTGGAAA TCGCTATGCA ATCACGTAAA 
GAAAATGATG GTCGGACATT GAAGCAGGTT ATTCAGTTGA CTTTTGAGAA GGCCACTGAT 
GAACCGAACT TTTCAAACAT GTATGCTCGA TTTGCTCGTA AAATGATGGA CAGTATTGAT 
GATTCAATCA GAGATGAAGG GGTATTAGAT AAGAATAACC AACCCGTACG GGGTGGCCTA 
CTTTTCAGAA AATATCTACT CAGTCGCTGT CAGGAAGATT TTGAGCGTGG TTGGAAAGCT 
AATTTACCAT CTGGTAAAGC GGGGGAAGCT GAAATTATGT CAGATGAATA TTATGTAGCT 
GCTGCCATTA AGCGTAGAGG TCTTGGCTTG GTACGCTTTA TTGGTGAATT ATTTAAACTC 
AGCATGTTGT CTGAAAAAAT CATGCATGAG TGTATTAAAC GTTTGTTGGG TAATGTAACA 
GACCCAGAAG AAGAAGAGAT TGAAAGTTTG TGCAGACTGT TAATGACTGT TGGTGTAAAT 
ATTGATGCTA CTGAAAAAGG GCATGCCGCT ATGGATGTAT ATGTGCTTCG GATGGAGACA 
ATTACTAAAA TTCCTAACCT ACCCTCTCGT ATCAAATTTA TGCTTATGGA TGTGATGGAT 
TCTCGTAAGA ACGGATGGGC TGTTAAAAAT GAAGTCGAAA AAGGACCTAA GACAATTGCT 
GAAATTCACG AAGAAGCAGA ACGTAAGAAA GCTTTGGCCG AAAGTCAACG CCCGTCGTCT 
GGTAGAATGC ACGGTCGGGA TATGAATCGT GGTGATTCTC GGATGGGCGG TCGAGGAAGC 
AATCCACCAT TTAGCTCTAG TGATTGGTCT AATAACAAAG ATGGTTATGC TAGACTTGGA 
CAAGGTATAA GAGGTCTCAA ATCTGGTACT CAGGGTTCTC ACGGTCCCAC AAGTTTGAGT 
TCAATGCTTA AAGGTGGAAG CGTTAGTAGA ACGCCTTCTC GTCAAAACAG TGCCTTAAGA 
AGGGAACAGT CTGTTCGTGC ACCACCATCT AACGTCGCGG TTACTTCTGC TAATTCTTTC 
GAATTACTTG AAGAGCATGA CCATGATAAT GATGGGGGTC AAAAAGATTC TAACTCAAAA 
ACTAGTTCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_594602.1
CDS	334..4545
Translation	MSSKPPSNTPKFSYARALASSQSNKSNSTKASENNTATAEKQAVKPSGVEPTNTSRANAQKKTESTGKITSEADTEKYNSSKSPVNKEGSVEKKSSEKSSTNNKPWRGDNTSKPSANSSAERTSSQHQKPETSSQIGKDNAAPVENVNEKSTSQETAPPVSTVPIQFGSITRNAAIPSKPKVSGNMQNKSGVSSYSSKSQSVNSSVTSNPPHTEEPVAAKPEASSTATKGPRPTTSASNTNTSPANGAPTNKPSTDINTTDPATQTTQVSASNSPALSGSSTPSNTSSRSNRQNHGNFSEKRHYDRYGNSHPSYNKYSHYQHGFNYNNSGNNRNESGHPRFRNSRRNYNNQGAYPTYMSNGRSANQSPRNNPQNVNNGSTPIQIPVSLQTPYGQVYGQPQYIVDPNMVQYGPILQPGYVPQYYPVYHQTPYTQNFPNMSRSGSQVSDQVVESPNSSTLSPRNGFAPIVKQQKKSSALKIVNPVTHTEVVVPQKNASSPNPSETNSRAETPTAAPPQISEEEASQRKDAIKLAIQQRIQEKAEAEAKRKAEEKARLEAEENAKREAEEQAKREAEEKAKREAEEKAKREAEEKAKREAEENAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEENAKREAEEKAKREAEENAKREAEEKVKRETEENAKRKAEEEGKREADKNPEIKSSAPLASSEANVDTSKQTNATEPEVVDKTKVEKLKASEGKSTSSLSSPSHSTSSKRDLLSGLESLSLKTNPKSEQCLESLLNSQFITDFSALVYPSTIKPPSTEEALKAGKYEYDVPFLLQFQSVYTDKPMKGWDERMKETVASAFSDKSSRGMYSSSRQSSRSGSNTHSHAGPGFGGPSERKGISRLGIDRGFSSSGAGFGSGSNYKSAPSRGVSHHGHGGMSGSHRGSQRGSRRGGGERDKPDPSSLTIPVDQVAPLQLSANRWQPKKLTEKPAETKGEDEEALLPPEVVQRKVKGSLNKMTLEKFDKISDQILEIAMQSRKENDGRTLKQVIQLTFEKATDEPNFSNMYARFARKMMDSIDDSIRDEGVLDKNNQPVRGGLLFRKYLLSRCQEDFERGWKANLPSGKAGEAEIMSDEYYVAAAIKRRGLGLVRFIGELFKLSMLSEKIMHECIKRLLGNVTDPEEEEIESLCRLLMTVGVNIDATEKGHAAMDVYVLRMETITKIPNLPSRIKFMLMDVMDSRKNGWAVKNEVEKGPKTIAEIHEEAERKKALAESQRPSSGRMHGRDMNRGDSRMGGRGSNPPFSSSDWSNNKDGYARLGQGIRGLKSGTQGSHGPTSLSSMLKGGSVSRTPSRQNSALRREQSVRAPPSNVAVTSANSFELLEEHDHDNDGGQKDSNSKTSS

Target ORF information:

RefSeq Version	NM_001020030.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe translation initiation factor eIF4G (tif471), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001020030.2

ORF Insert Sequence:

ATGTCTTCAA AACCACCGTC AAATACTCCA AAGTTTAGTT ATGCTCGTGC CTTGGCCAGC 
AGTCAATCCA ATAAAAGCAA TAGCACTAAA GCTTCAGAAA ATAATACTGC AACTGCTGAA 
AAGCAGGCAG TAAAGCCTTC AGGGGTGGAG CCAACGAATA CCTCTCGTGC TAATGCCCAA 
AAGAAAACAG AGTCCACTGG AAAGATTACT TCAGAGGCTG ACACTGAAAA GTACAACTCA 
AGCAAATCTC CTGTCAACAA GGAAGGATCG GTGGAAAAAA AATCTTCTGA AAAATCTTCA 
ACCAATAATA AGCCATGGCG CGGCGATAAT ACTTCAAAAC CATCGGCCAA TAGCTCTGCT 
GAACGAACGA GCTCACAGCA TCAGAAGCCT GAAACTTCAT CCCAAATTGG TAAAGACAAT 
GCAGCTCCCG TGGAAAATGT AAATGAGAAA TCTACCTCCC AAGAAACAGC ACCTCCTGTA 
TCCACTGTGC CAATTCAGTT TGGTTCGATT ACCAGAAATG CGGCTATCCC TTCAAAACCA 
AAGGTTTCTG GTAACATGCA AAATAAGAGT GGCGTGTCTT CTTATAGTTC CAAAAGCCAG 
TCTGTTAACT CCTCGGTTAC ATCCAATCCT CCTCATACAG AAGAACCTGT TGCTGCTAAG 
CCGGAGGCTT CTTCAACCGC CACTAAAGGA CCTCGTCCTA CCACGAGTGC ATCCAACACC 
AATACCTCTC CTGCTAATGG CGCACCGACT AATAAACCTT CAACCGATAT CAATACCACT 
GATCCCGCCA CCCAAACTAC CCAAGTTTCA GCGTCCAACT CTCCTGCATT GTCAGGAAGT 
AGTACACCAT CAAACACATC TTCTAGATCT AATCGTCAAA ATCACGGAAA CTTTTCCGAA 
AAGCGGCACT ACGACCGTTA CGGAAATTCT CATCCTTCTT ACAATAAATA TTCTCATTAT 
CAGCATGGTT TTAATTATAA TAACTCGGGA AATAATAGGA ATGAGTCAGG GCATCCTCGT 
TTTCGAAACT CTAGGAGAAA CTATAACAAT CAAGGTGCCT ATCCTACGTA TATGTCTAAT 
GGACGTAGTG CCAATCAAAG CCCTCGGAAC AATCCCCAAA ACGTAAACAA TGGTAGTACA 
CCAATTCAAA TTCCAGTTTC TTTACAGACT CCTTACGGAC AAGTATATGG GCAACCACAA 
TACATCGTGG ATCCCAATAT GGTGCAGTAT GGTCCTATCC TTCAACCTGG TTATGTTCCT 
CAATATTACC CAGTTTACCA TCAGACACCC TATACTCAGA ACTTTCCTAA TATGTCAAGA 
AGCGGCTCTC AAGTTTCTGA TCAAGTTGTT GAATCTCCTA ATTCATCTAC ACTTAGCCCC 
AGAAATGGAT TTGCCCCTAT AGTGAAGCAA CAGAAAAAGA GTAGCGCATT GAAGATTGTC 
AATCCCGTAA CACATACAGA AGTAGTGGTT CCGCAAAAAA ATGCGTCGTC TCCTAATCCC 
TCTGAAACTA ACTCTCGTGC TGAAACACCA ACTGCTGCTC CTCCTCAGAT TTCAGAAGAG 
GAAGCTTCAC AGCGAAAAGA TGCTATTAAG CTTGCCATTC AACAGCGTAT CCAGGAAAAA 
GCGGAAGCTG AAGCAAAGCG TAAAGCCGAA GAGAAGGCCA GACTAGAAGC GGAAGAAAAT 
GCCAAACGTG AAGCGGAAGA ACAAGCAAAG CGTGAAGCGG AAGAAAAGGC TAAACGTGAA 
GCTGAAGAGA AGGCCAAACG TGAAGCTGAA GAGAAGGCCA AACGTGAAGC TGAAGAGAAT 
GCCAAACGTG AAGCTGAGGA GAAGGCTAAG CGCGAAGCTG AAGAGAAGGC TAAACGTGAA 
GCTGAAGAAA AAGCCAAACG TGAAGCTGAA GAAAAGGCTA AGCGTGAAGC TGAAGAAAAG 
GCTAAACGCG AAGCTGAAGA AAAGGCTAAG CGTGAAGCTG AAGAAAAGGC TAAGCGCGAA 
GCTGAAGAGA ATGCTAAACG TGAAGCTGAG GAGAAGGCTA AACGTGAAGC TGAAGAAAAC 
GCTAAACGTG AAGCTGAAGA GAAGGTTAAA CGTGAGACTG AGGAAAATGC TAAACGGAAA 
GCTGAGGAAG AGGGTAAACG GGAAGCTGAT AAAAACCCTG AAATCAAATC AAGTGCACCC 
CTTGCTTCGA GTGAAGCTAA CGTTGATACC AGTAAGCAAA CAAATGCTAC CGAACCTGAA 
GTGGTTGACA AAACAAAAGT GGAGAAGCTT AAGGCATCTG AAGGAAAATC GACTTCTTCG 
CTTTCTTCTC CCTCTCATTC GACCTCTTCT AAACGGGACT TGCTATCTGG TCTGGAAAGC 
CTCAGTCTGA AAACTAACCC CAAATCTGAG CAGTGCTTAG AATCATTGTT AAATTCTCAA 
TTTATAACCG ACTTTTCTGC TTTGGTTTAT CCTTCCACTA TCAAACCACC ATCTACTGAG 
GAAGCCTTAA AAGCTGGTAA ATATGAGTAT GATGTTCCTT TCCTTTTACA GTTTCAAAGC 
GTTTATACTG ATAAGCCAAT GAAAGGTTGG GACGAAAGGA TGAAGGAAAC AGTAGCATCC 
GCTTTTAGTG ATAAAAGCTC TCGTGGAATG TACAGCTCTA GTCGCCAAAG CTCTCGATCC 
GGCTCAAATA CTCACTCCCA TGCTGGCCCT GGTTTTGGTG GTCCTTCAGA GAGAAAGGGC 
ATTTCAAGGC TTGGAATCGA TCGAGGTTTC AGCTCCTCTG GTGCTGGATT TGGATCGGGT 
AGTAATTATA AGAGCGCTCC TAGTCGCGGT GTAAGTCATC ACGGACACGG TGGTATGTCT 
GGGTCTCATC GTGGTTCCCA GCGTGGTTCT CGCCGCGGAG GTGGTGAGCG TGATAAACCC 
GATCCATCTA GTCTAACTAT ACCTGTTGAT CAGGTAGCAC CTCTTCAATT ATCCGCAAAC 
CGCTGGCAAC CTAAGAAACT TACTGAAAAA CCTGCTGAAA CTAAGGGTGA GGATGAAGAA 
GCACTTCTTC CTCCCGAGGT CGTGCAGCGT AAGGTTAAAG GTTCTTTGAA CAAGATGACA 
TTAGAGAAGT TTGATAAAAT ATCTGACCAG ATTTTGGAAA TCGCTATGCA ATCACGTAAA 
GAAAATGATG GTCGGACATT GAAGCAGGTT ATTCAGTTGA CTTTTGAGAA GGCCACTGAT 
GAACCGAACT TTTCAAACAT GTATGCTCGA TTTGCTCGTA AAATGATGGA CAGTATTGAT 
GATTCAATCA GAGATGAAGG GGTATTAGAT AAGAATAACC AACCCGTACG GGGTGGCCTA 
CTTTTCAGAA AATATCTACT CAGTCGCTGT CAGGAAGATT TTGAGCGTGG TTGGAAAGCT 
AATTTACCAT CTGGTAAAGC GGGGGAAGCT GAAATTATGT CAGATGAATA TTATGTAGCT 
GCTGCCATTA AGCGTAGAGG TCTTGGCTTG GTACGCTTTA TTGGTGAATT ATTTAAACTC 
AGCATGTTGT CTGAAAAAAT CATGCATGAG TGTATTAAAC GTTTGTTGGG TAATGTAACA 
GACCCAGAAG AAGAAGAGAT TGAAAGTTTG TGCAGACTGT TAATGACTGT TGGTGTAAAT 
ATTGATGCTA CTGAAAAAGG GCATGCCGCT ATGGATGTAT ATGTGCTTCG GATGGAGACA 
ATTACTAAAA TTCCTAACCT ACCCTCTCGT ATCAAATTTA TGCTTATGGA TGTGATGGAT 
TCTCGTAAGA ACGGATGGGC TGTTAAAAAT GAAGTCGAAA AAGGACCTAA GACAATTGCT 
GAAATTCACG AAGAAGCAGA ACGTAAGAAA GCTTTGGCCG AAAGTCAACG CCCGTCGTCT 
GGTAGAATGC ACGGTCGGGA TATGAATCGT GGTGATTCTC GGATGGGCGG TCGAGGAAGC 
AATCCACCAT TTAGCTCTAG TGATTGGTCT AATAACAAAG ATGGTTATGC TAGACTTGGA 
CAAGGTATAA GAGGTCTCAA ATCTGGTACT CAGGGTTCTC ACGGTCCCAC AAGTTTGAGT 
TCAATGCTTA AAGGTGGAAG CGTTAGTAGA ACGCCTTCTC GTCAAAACAG TGCCTTAAGA 
AGGGAACAGT CTGTTCGTGC ACCACCATCT AACGTCGCGG TTACTTCTGC TAATTCTTTC 
GAATTACTTG AAGAGCATGA CCATGATAAT GATGGGGGTC AAAAAGATTC TAACTCAAAA 
ACTAGTTCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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tif471 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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