hsf1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	hsf1
Entrez Gene ID	2541888
Full Name	transcription factor Hsf1
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001020275.2	NP_594846.1	transcription factor Hsf1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We identify the Ssa2-Mas5/Hsp70-Hsp40 chaperone complex as repressor of the heat shock transcription factor Hsf1
Title: Hsp70-Hsp40 chaperone complex functions in controlling polarized growth by repressing Hsf1-driven heat stress-associated transcription.

Hsf1 extends chronological lifespan of S. pombe when overexpressed. Hsf1 binds to the upstream DNA region of ecl2 (+) after heat shock in S. pombe.
Title: hsf1 (+) extends chronological lifespan through Ecl1 family genes in fission yeast.

The following hsf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hsf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc103821	NM_001020275.2 Latest version!	Schizosaccharomyces pombe transcription factor Hsf1 (hsf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hsf1 ( NM_001020275.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_594846.1 Schizosaccharomyces pombe transcription factor Hsf1 (hsf1), mRNA.

Price & Availability↑

CloneID	OSc103821
Clone ID Related Accession (Same CDS sequence)	NM_001020275.2
Accession Version	NM_001020275.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	transcription factor Hsf1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001020275.1.

ATGATTCAGA CTGCATCGAC AATTTCAAGC AATGGCGGAA CGAATCAGGG AATGGAGTCC 
AGCAGCGCAA ATTCACCGGA GATGAATGGG ACACAAAATT CGATGTCCGT AGGGATGTCG 
GGCTCAGGTT CTTCACAAAA TAGGAAAATC ACACAGTTTA GTAATAAACT GTACAATATG 
GTGAACGACT CGTCGACAGA TTCCTTAATT CGATGGAGCG ACCGGGGTGA TTCGTTTTTG 
GTTATAGGGC ATGAAGATTT TGCTAAGCTG GTTCTTCCAC GGTATTTCAA GCACAATAAT 
TTTTCTAGTT TTGTTCGGCA ATTAAACATG TACGGCTTTC ATAAAGTCCC TCATATACAG 
CAAGGTGTTT TACAATCCGA TAGTCCCAAC GAGCTTCTCG AGTTTGCAAA TCCAAACTTT 
CAACGTGACC AACCTGAATT ACTATGCCTA GTAACGCGAA AAAAGGCCGG TAGCCAACCC 
GTTGAAGAAT CAAATACCAG TTTAGACATG TCTACGATAT CCTCTGAGCT ACAGAATATT 
CGCATCCAGC AGATGAATTT GTCGAACGAA CTCAGTAGGA TCCAAGTCGA TAACGCAGCT 
CTTTGGCAGG AAAATATGGA AAATCGCGAG AGACAACGTC GCCATCAAGA GACGATTGAT 
AAGATCCTTC GTTTTCTTGC CAGTGTATAT TTGGATGGTA AACAGAAGCC ACCTAGCAAA 
GTTATGCCCA AAAGTCGTCG GCTCTTATTA GAAGCTAAAT ATCCTACGGT TTCACCTACG 
AATGAACCGT CTGCGCATTC AAGGCCTTCA CCTCAAGGTA CTACTGCTAA TTCTAGTAGT 
GCTAGTATCT CCTCTTTACA CAATACGACT CCTGATGGAG AGGGCAAATA CAGATCCGTT 
CAAAATGGAC GAGCTTTAAA TTATGTTTCT TCTTTTAATT CGGATTCGCA TTCACCGAAG 
GATTATATTT CTCAAAGCTA TACGAATGAG CCAGGACTTA AAAAGGAATC TGCCGATTCC 
TTTAACAATT CGATTGACTC ATACATTTCT CCCAATCAAT CACCTAATAC GGATGTACCC 
TCATTAAATC GAGATGATAC CACTGATCCT AAAGTCGTGA ACACTGGTGA TATTATCAAT 
ATGTTAGATG ATGCCAATAG TATTGAAGGA TCGAATATGA ATTCCCTGTC ACCTCTTCTT 
TTCGACTATC CCAATTCACT TTATCCGGTT AACAACACTT CTTCTGAACA GCACCATAAC 
TCTTACAGAG GATCGGTGTC AAATTCCCAA CCTTCTGGAA ATTTGTCGGA ATCGACTAAT 
TTGCAACCTG TTCAACCAGT TGATTATATG TCAAATTCGA ATCCGTCCTA CGGTTCCTAT 
AATGCAGAAG ATCAACTTAC AAACTTCCAC CCAGGCTATG CCATGGATCA AAAAAGAATT 
TCTAAGTTAT CAGATGGAAT TACGAAGCAG GATCAGAATA TACAAGCGTT GGCGGATATT 
TTAGGTATCC CGCTGGGTGA CGGAAAGATT GATGATGCTG GGTTTTCTGC TAATTCACCC 
ACCAACTTGA ACTTGCCTGT ATCATCTGAT CTTGACAGCG TATTAAACAT TCCTCCAAAT 
GAGGATGTTT TCCCGGACTC AAATCCCGTT TTTGATGAAT TTACAAATAT TTCGAATCTA 
ACGTCACCCA TTGAAGCATC CAACGGGAAC ACGTTTGGTT CGAATCCCGT TTCTTTGCCT 
AATCAGCAGA AATCAGTTAA TCCATCTTTA ATGACAGTGT CCTCCCCTAG ACAAGTAAGA 
AAGAAGAGGA AATCAAGCAT TGGTGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_594846.1
CDS	203..2032
Translation	MIQTASTISSNGGTNQGMESSSANSPEMNGTQNSMSVGMSGSGSSQNRKITQFSNKLYNMVNDSSTDSLIRWSDRGDSFLVIGHEDFAKLVLPRYFKHNNFSSFVRQLNMYGFHKVPHIQQGVLQSDSPNELLEFANPNFQRDQPELLCLVTRKKAGSQPVEESNTSLDMSTISSELQNIRIQQMNLSNELSRIQVDNAALWQENMENRERQRRHQETIDKILRFLASVYLDGKQKPPSKVMPKSRRLLLEAKYPTVSPTNEPSAHSRPSPQGTTANSSSASISSLHNTTPDGEGKYRSVQNGRALNYVSSFNSDSHSPKDYISQSYTNEPGLKKESADSFNNSIDSYISPNQSPNTDVPSLNRDDTTDPKVVNTGDIINMLDDANSIEGSNMNSLSPLLFDYPNSLYPVNNTSSEQHHNSYRGSVSNSQPSGNLSESTNLQPVQPVDYMSNSNPSYGSYNAEDQLTNFHPGYAMDQKRISKLSDGITKQDQNIQALADILGIPLGDGKIDDAGFSANSPTNLNLPVSSDLDSVLNIPPNEDVFPDSNPVFDEFTNISNLTSPIEASNGNTFGSNPVSLPNQQKSVNPSLMTVSSPRQVRKKRKSSIGA

Target ORF information:

RefSeq Version	NM_001020275.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe transcription factor Hsf1 (hsf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001020275.2

ORF Insert Sequence:

ATGATTCAGA CTGCATCGAC AATTTCAAGC AATGGCGGAA CGAATCAGGG AATGGAGTCC 
AGCAGCGCAA ATTCACCGGA GATGAATGGG ACACAAAATT CGATGTCCGT AGGGATGTCG 
GGCTCAGGTT CTTCACAAAA TAGGAAAATC ACACAGTTTA GTAATAAACT GTACAATATG 
GTGAACGACT CGTCGACAGA TTCCTTAATT CGATGGAGCG ACCGGGGTGA TTCGTTTTTG 
GTTATAGGGC ATGAAGATTT TGCTAAGCTG GTTCTTCCAC GGTATTTCAA GCACAATAAT 
TTTTCTAGTT TTGTTCGGCA ATTAAACATG TACGGCTTTC ATAAAGTCCC TCATATACAG 
CAAGGTGTTT TACAATCCGA TAGTCCCAAC GAGCTTCTCG AGTTTGCAAA TCCAAACTTT 
CAACGTGACC AACCTGAATT ACTATGCCTA GTAACGCGAA AAAAGGCCGG TAGCCAACCC 
GTTGAAGAAT CAAATACCAG TTTAGACATG TCTACGATAT CCTCTGAGCT ACAGAATATT 
CGCATCCAGC AGATGAATTT GTCGAACGAA CTCAGTAGGA TCCAAGTCGA TAACGCAGCT 
CTTTGGCAGG AAAATATGGA AAATCGCGAG AGACAACGTC GCCATCAAGA GACGATTGAT 
AAGATCCTTC GTTTTCTTGC CAGTGTATAT TTGGATGGTA AACAGAAGCC ACCTAGCAAA 
GTTATGCCCA AAAGTCGTCG GCTCTTATTA GAAGCTAAAT ATCCTACGGT TTCACCTACG 
AATGAACCGT CTGCGCATTC AAGGCCTTCA CCTCAAGGTA CTACTGCTAA TTCTAGTAGT 
GCTAGTATCT CCTCTTTACA CAATACGACT CCTGATGGAG AGGGCAAATA CAGATCCGTT 
CAAAATGGAC GAGCTTTAAA TTATGTTTCT TCTTTTAATT CGGATTCGCA TTCACCGAAG 
GATTATATTT CTCAAAGCTA TACGAATGAG CCAGGACTTA AAAAGGAATC TGCCGATTCC 
TTTAACAATT CGATTGACTC ATACATTTCT CCCAATCAAT CACCTAATAC GGATGTACCC 
TCATTAAATC GAGATGATAC CACTGATCCT AAAGTCGTGA ACACTGGTGA TATTATCAAT 
ATGTTAGATG ATGCCAATAG TATTGAAGGA TCGAATATGA ATTCCCTGTC ACCTCTTCTT 
TTCGACTATC CCAATTCACT TTATCCGGTT AACAACACTT CTTCTGAACA GCACCATAAC 
TCTTACAGAG GATCGGTGTC AAATTCCCAA CCTTCTGGAA ATTTGTCGGA ATCGACTAAT 
TTGCAACCTG TTCAACCAGT TGATTATATG TCAAATTCGA ATCCGTCCTA CGGTTCCTAT 
AATGCAGAAG ATCAACTTAC AAACTTCCAC CCAGGCTATG CCATGGATCA AAAAAGAATT 
TCTAAGTTAT CAGATGGAAT TACGAAGCAG GATCAGAATA TACAAGCGTT GGCGGATATT 
TTAGGTATCC CGCTGGGTGA CGGAAAGATT GATGATGCTG GGTTTTCTGC TAATTCACCC 
ACCAACTTGA ACTTGCCTGT ATCATCTGAT CTTGACAGCG TATTAAACAT TCCTCCAAAT 
GAGGATGTTT TCCCGGACTC AAATCCCGTT TTTGATGAAT TTACAAATAT TTCGAATCTA 
ACGTCACCCA TTGAAGCATC CAACGGGAAC ACGTTTGGTT CGAATCCCGT TTCTTTGCCT 
AATCAGCAGA AATCAGTTAA TCCATCTTTA ATGACAGTGT CCTCCCCTAG ACAAGTAAGA 
AAGAAGAGGA AATCAAGCAT TGGTGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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hsf1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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