SPAC227.14 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following SPAC227.14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAC227.14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSj02743 NM_001018368.2
Latest version!
Schizosaccharomyces pombe 972h- uridine kinase (predicted) (SPAC227.14), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OSc103240 NM_001355982.1
Latest version!
Schizosaccharomyces pombe putative uridine kinase (SPAC227.14), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSj02743
    Clone ID Related Accession (Same CDS sequence) NM_001018368.2
    Accession Version NM_001018368.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 708bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2013-04-30
    Organism Schizosaccharomyces pombe 972h-
    Product uridine kinase (predicted)
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001018368.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGCTTTAC AAGTAGATGC CCCAGATGTA TCGTCTTATG ACGAACTTTA CAACCGTGCA 
    GTTGAATTGT TGAAGCACCA GCAACGAGTT CTAATCGGTT TGGCTGGAGG TCCTGGTTCA
    GGCAAGTCAA CATTATGTGC TATTCTCGCA AAGGCATGGA ATGAAAGATT TGGATCGGAG
    ATCGTAAAAA TAATTCCAAT GGATGGCTTT CATTATTCGT TGGAAGAGCT TGATCGATTC
    GACAACCCAG AAAAGGCCCG GGCTCTTCGT GGAGCAGAAT GGACGTTTGA TGCAGACTTA
    TTCTACTCCT TAGTTCGGCT TATGAAAAAG ATCACTGACA GAGAGCTCTA TGCCCCTTCA
    TTTGATCATG CAATTGGTGA CCCTGTAGTC GACGATATTT GCGTTGAGCC CAAAAATCGA
    ATTTTAATTT TTGAAGGTAA TTACCTTTTG CTCAACAAAC CTCCATGGTC AGACGCATGC
    AAATTATATG ACATTAAGGC ATACCTCCCT GTAGAGCACT CAGTGGCCCG TGCTCGTGTT
    GCGCATCGTC ACTTGGTTTC AGGATTATGC GCAACAGAAG AAGAGGCAAT TGAGAGGACC
    GACAGAAATG ATATGATTAA TCTCACTTTT GTCGAGAAGA ACATGGTTAC CCCAGATATT
    GTATTACAGC AGCTACGTTT GAAAACCGTT AAAACATCTA GTTTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_592968.1
    CDS760..1467
    Translation

    Target ORF information:

    RefSeq Version NM_001018368.2
    Organism Schizosaccharomyces pombe 972h-
    Definition Schizosaccharomyces pombe 972h- uridine kinase (predicted) (SPAC227.14), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001018368.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGCTTTAC AAGTAGATGC CCCAGATGTA TCGTCTTATG ACGAACTTTA CAACCGTGCA 
    GTTGAATTGT TGAAGCACCA GCAACGAGTT CTAATCGGTT TGGCTGGAGG TCCTGGTTCA
    GGCAAGTCAA CATTATGTGC TATTCTCGCA AAGGCATGGA ATGAAAGATT TGGATCGGAG
    ATCGTAAAAA TAATTCCAAT GGATGGCTTT CATTATTCGT TGGAAGAGCT TGATCGATTC
    GACAACCCAG AAAAGGCCCG GGCTCTTCGT GGAGCAGAAT GGACGTTTGA TGCAGACTTA
    TTCTACTCCT TAGTTCGGCT TATGAAAAAG ATCACTGACA GAGAGCTCTA TGCCCCTTCA
    TTTGATCATG CAATTGGTGA CCCTGTAGTC GACGATATTT GCGTTGAGCC CAAAAATCGA
    ATTTTAATTT TTGAAGGTAA TTACCTTTTG CTCAACAAAC CTCCATGGTC AGACGCATGC
    AAATTATATG ACATTAAGGC ATACCTCCCT GTAGAGCACT CAGTGGCCCG TGCTCGTGTT
    GCGCATCGTC ACTTGGTTTC AGGATTATGC GCAACAGAAG AAGAGGCAAT TGAGAGGACC
    GACAGAAATG ATATGATTAA TCTCACTTTT GTCGAGAAGA ACATGGTTAC CCCAGATATT
    GTATTACAGC AGCTACGTTT GAAAACCGTT AAAACATCTA GTTTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSc103240
    Clone ID Related Accession (Same CDS sequence) NM_001355982.1
    Accession Version NM_001355982.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 708bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative uridine kinase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGCTTTAC AAGTAGATGC CCCAGATGTA TCGTCTTATG ACGAACTTTA CAACCGTGCA 
    GTTGAATTGT TGAAGCACCA GCAACGAGTT CTAATCGGTT TGGCTGGAGG TCCTGGTTCA
    GGCAAGTCAA CATTATGTGC TATTCTCGCA AAGGCATGGA ATGAAAGATT TGGATCGGAG
    ATCGTAAAAA TAATTCCAAT GGATGGCTTT CATTATTCGT TGGAAGAGCT TGATCGATTC
    GACAACCCAG AAAAGGCCCG GGCTCTTCGT GGAGCAGAAT GGACGTTTGA TGCAGACTTA
    TTCTACTCCT TAGTTCGGCT TATGAAAAAG ATCACTGACA GAGAGCTCTA TGCCCCTTCA
    TTTGATCATG CAATTGGTGA CCCTGTAGTC GACGATATTT GCGTTGAGCC CAAAAATCGA
    ATTTTAATTT TTGAAGGTAA TTACCTTTTG CTCAACAAAC CTCCATGGTC AGACGCATGC
    AAATTATATG ACATTAAGGC ATACCTCCCT GTAGAGCACT CAGTGGCCCG TGCTCGTGTT
    GCGCATCGTC ACTTGGTTTC AGGATTATGC GCAACAGAAG AAGAGGCAAT TGAGAGGACC
    GACAGAAATG ATATGATTAA TCTCACTTTT GTCGAGAAGA ACATGGTTAC CCCAGATATT
    GTATTACAGC AGCTACGTTT GAAAACCGTT AAAACATCTA GTTTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001342927.1
    CDS655..1362
    Translation

    Target ORF information:

    RefSeq Version NM_001355982.1
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative uridine kinase (SPAC227.14), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001355982.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGCTTTAC AAGTAGATGC CCCAGATGTA TCGTCTTATG ACGAACTTTA CAACCGTGCA 
    GTTGAATTGT TGAAGCACCA GCAACGAGTT CTAATCGGTT TGGCTGGAGG TCCTGGTTCA
    GGCAAGTCAA CATTATGTGC TATTCTCGCA AAGGCATGGA ATGAAAGATT TGGATCGGAG
    ATCGTAAAAA TAATTCCAAT GGATGGCTTT CATTATTCGT TGGAAGAGCT TGATCGATTC
    GACAACCCAG AAAAGGCCCG GGCTCTTCGT GGAGCAGAAT GGACGTTTGA TGCAGACTTA
    TTCTACTCCT TAGTTCGGCT TATGAAAAAG ATCACTGACA GAGAGCTCTA TGCCCCTTCA
    TTTGATCATG CAATTGGTGA CCCTGTAGTC GACGATATTT GCGTTGAGCC CAAAAATCGA
    ATTTTAATTT TTGAAGGTAA TTACCTTTTG CTCAACAAAC CTCCATGGTC AGACGCATGC
    AAATTATATG ACATTAAGGC ATACCTCCCT GTAGAGCACT CAGTGGCCCG TGCTCGTGTT
    GCGCATCGTC ACTTGGTTTC AGGATTATGC GCAACAGAAG AAGAGGCAAT TGAGAGGACC
    GACAGAAATG ATATGATTAA TCTCACTTTT GTCGAGAAGA ACATGGTTAC CCCAGATATT
    GTATTACAGC AGCTACGTTT GAAAACCGTT AAAACATCTA GTTTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L