rga4 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	rga4
Entrez Gene ID	2540593
Full Name	Rho-type GTPase activating protein Rga4
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001021656.2	NP_595756.1	Rho-type GTPase-activating protein Rga4

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rga4 modulates the activity of the fission yeast cell integrity MAPK pathway by acting as a Rho2 GTPase-activating protein
Title: Rga4 modulates the activity of the fission yeast cell integrity MAPK pathway by acting as a Rho2 GTPase-activating protein.

Results indicate that Rga4p functionally interacts with For3p and has a novel function in the control of cell diameter and cell growth.
Title: Regulation of cell diameter, For3p localization, and cell symmetry by fission yeast Rho-GAP Rga4p.

The following rga4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rga4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc100011	NM_001021656.2 Latest version!	Schizosaccharomyces pombe Rho-type GTPase-activating protein Rga4 (rga4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rga4 ( NM_001021656.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_595756.1 Schizosaccharomyces pombe Rho-type GTPase-activating protein Rga4 (rga4), mRNA.

Price & Availability↑

CloneID	OSc100011
Clone ID Related Accession (Same CDS sequence)	NM_001021656.2
Accession Version	NM_001021656.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	Rho-type GTPase-activating protein Rga4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021656.1.

ATGAATTCGG GTACGACACT TCATCTGGAC AGACTTAGTT TGGAGACGCC ATCCGAACGT 
ACTTGTTTTT GTATTAAATG TTGGGAAAGT GTTCCCAGTA CCTCTCAAGT TTGGTTCGGT 
GGGAAATGTT GGCATTCCGA TTGTTTTAAA TGTGTGAACT GCAACAAAAA ATTAGACCCT 
TCCTCTGAAG ATTTTTCTCA AGACGATCAA AAGCAAATAT TCTGTAAATT ATGTGTTGAT 
ATCTGCAACG GTTGCTCAAC TCCTATTTGC GAGTTCAACG CAGTAAGCAA TAGTCAAGCT 
AATCATCCTT GTTGCTCCAA TTGTCGAGCT TTCATTAATT CAAATGCTTT TGGCAAGTTC 
AAAGGTCAAC ATTACTGCCT TCCTTGTTTG CGTAAACAAG ATGAAGAGAA TCAAAAGGCT 
GTTATGGAAA GCGCATCAGG GTTTATTCCT CTTAGGAATA TCGCACAAAC TGCGGAAAAC 
ACTTCTTTAA CTGGGCAAAA CCAAGGCTTT CAAAACCCGC ATTTTATAGC GTCTGATTCT 
CCATCCTCCG TATTATCTGG TCGAATGCAA AATACCAGCT CTCCTACAAA CTCTCTCCGT 
CCTCAATTAA AAGTTCAAAC TAATGGTTAT GAAACACCTG GTGATATAAA CTCCGCAAAA 
TCTTACAAAG ACATCCAAAA GGACTTCTTA CTTAAACCCA AAAATTCTTT CGTTAAAACC 
TCTCCCTCTC CACTAGCTAA TGGTCCCTCA TTTCGTTCAG CTAATGCCTC TCCATTTGAT 
TCGTGTGACT CTTTTCATTC TGGAAGTTCG ATACCAATTG AACCAGTTTC TTCAAGGCAA 
AGCTCAGTAG TAAATAATAA TAGCGTGCAG CAACCTGTTG CTTATCACGC CTTTGTTCAA 
TCACCAACCG AAAATGGTAC GCTTCCTCAA CTTCCTAAAA ATGAAAGCGT TGTTAATCCT 
CCTCCACTTC GCCGCTCTTC TACTATGAAT TACAAATCTG TTTCGACAAC AACATCACCA 
TCTAAATATG GATATGTGTC TGGCAGAATA GCTCTATCTC CTATCCATTT AAGGGGTGCT 
CTTCGTGATG TCACAAATAA ATGTAATTTA AAGGTTCCTA GAAATCGAAA CAGTTTATCT 
AATCTTGACG AATATTACGT TAACGGATTG GAAAGCGACG AAACTCCTAC AAAGGCTAGA 
TTTCCTAGAT ATCCTACGGT ATTTAATAAG TTAGATGACA AACGACTTAG TAGCGAACCA 
AACGGCTTGA AGAAAAGATT GACGAACTCT TCAAATTATG AGGCTTCCCC GCGGGCCAAA 
AGTTTAAATT TGTCGCAAGT TAGTTTGCAT CAAGCATGTG AACCTGAATA CAATCGAAGT 
TCTTTGGTTA GAGCAAGTGA TGTGTTTACT TCAAACGTAT TTGATGCTAC AGAGGAAAGC 
GCAAATGAAC TCGCCATTCG TATTTCAGAG CTTCAAGCTG AGGTTGGAAC CTTATTGTTG 
GAAGCGACTT CGTTGGCTTC TATCATAGAG CAGCAGACTC CCGTGTCCCC CGAAGCTTTC 
AAGCAAGAGT TGGTTGCAGA TTTAAATTAT AGATTAGATT ATTTACGGAA ATCTTTTCAA 
CCTGAACTGT CTACCTTACT TTTGAGGCGA GACGCTTTGT CTACGACTGT CTCTAAATTG 
CAAAACGCTT ACAGCACTGT AATGGAAGAA ACCGCATATC TGAATGTTAA GAACACAGAA 
CTGGTAAGCC TTAACAATCA ATTGGAGAGA GAATTGGCAT ACTTGAGGGA GCAGCAGCAT 
AAGAAGCGAA CTTCTTCCTC TTTCGGAATA TTTAATAATG ATAAAAAATC CAACCGAACG 
ATTTCAACCC CGTCTCCGCG AGAATCGTTT TCGCGTCTAC AAATGGTTGC ATCTAGCCTT 
GGATTTAGAC CCAAAGACAA TAAAGACAAG GAGAGTGGAG GGTATAACAA ACGGAACTCT 
AAAATAGATC TTAAGAAGTC ATTTTCTAAA CGGTTTCATT GGAAAAGAGA TTCTCCTCAC 
ATGTCGAGTA CTCCCAGTAT TTCTGAAGAA CATTTAGCTT CTGAAGAACA AGAGGATTTC 
GTTCCAGCGG TCGGTCACTG CAAACTTTGC GGTAAATACT CCAATGAGCT TAGAGCACAT 
TATCAGGATT GCGTTAGTTC TACTATAGAT CGTCAGTATC AATCTAAAAA AAGCGAATCT 
CCTGTTTGGG CTTTGAATCC TGACGACTTC GATCAAAACA GGCTAACACT CTTGAGAGTC 
CCGACGCTTA TCGTTTCTTG TATAAATTTT ATCGAGTCAT ACGGCATGGA TTTTGAAGGT 
CTGTATCGCA AATCAGGGGC AACTTCTCAA ATGAAAAAAA TTGTTGCGTT ACTAAGAAAT 
GAAGATACGG TACTTGATCC AAGTGAGGAT ATTTCTGCTG TCACTTCTGT ATTCAAGCAA 
TATCTTCGCA ACCTTCCTAA TCCAATTATT ACCTATGACC AATACTTTCC GTTCATCACA 
GCAGCAAATT GTGCCTCTTT CCAAGATAAA TTAGACGGTT TTATAATGGT CATCAAAAGT 
CTTCCTCCAG CTCATGCTGA AATTTTGCAG CTCATTATTA GACATTTAGC AAGGGTTGCT 
GCATATAGTC ATGCAAATCG AATGACATCC AAGAACCTTG CAGTAGTATT TTCCCCAACA 
TTAATACGCG ACCCCGATAA TTCCAGAGAC GTTATTGACA TGACTGTTAA GAATTACAGT 
CTTGCGTTTT TGATAGATCA TGTACATGAA GTCTTTGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_595756.1
CDS	261..3062
Translation	MNSGTTLHLDRLSLETPSERTCFCIKCWESVPSTSQVWFGGKCWHSDCFKCVNCNKKLDPSSEDFSQDDQKQIFCKLCVDICNGCSTPICEFNAVSNSQANHPCCSNCRAFINSNAFGKFKGQHYCLPCLRKQDEENQKAVMESASGFIPLRNIAQTAENTSLTGQNQGFQNPHFIASDSPSSVLSGRMQNTSSPTNSLRPQLKVQTNGYETPGDINSAKSYKDIQKDFLLKPKNSFVKTSPSPLANGPSFRSANASPFDSCDSFHSGSSIPIEPVSSRQSSVVNNNSVQQPVAYHAFVQSPTENGTLPQLPKNESVVNPPPLRRSSTMNYKSVSTTTSPSKYGYVSGRIALSPIHLRGALRDVTNKCNLKVPRNRNSLSNLDEYYVNGLESDETPTKARFPRYPTVFNKLDDKRLSSEPNGLKKRLTNSSNYEASPRAKSLNLSQVSLHQACEPEYNRSSLVRASDVFTSNVFDATEESANELAIRISELQAEVGTLLLEATSLASIIEQQTPVSPEAFKQELVADLNYRLDYLRKSFQPELSTLLLRRDALSTTVSKLQNAYSTVMEETAYLNVKNTELVSLNNQLERELAYLREQQHKKRTSSSFGIFNNDKKSNRTISTPSPRESFSRLQMVASSLGFRPKDNKDKESGGYNKRNSKIDLKKSFSKRFHWKRDSPHMSSTPSISEEHLASEEQEDFVPAVGHCKLCGKYSNELRAHYQDCVSSTIDRQYQSKKSESPVWALNPDDFDQNRLTLLRVPTLIVSCINFIESYGMDFEGLYRKSGATSQMKKIVALLRNEDTVLDPSEDISAVTSVFKQYLRNLPNPIITYDQYFPFITAANCASFQDKLDGFIMVIKSLPPAHAEILQLIIRHLARVAAYSHANRMTSKNLAVVFSPTLIRDPDNSRDVIDMTVKNYSLAFLIDHVHEVFA

Target ORF information:

RefSeq Version	NM_001021656.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe Rho-type GTPase-activating protein Rga4 (rga4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001021656.2

ORF Insert Sequence:

ATGAATTCGG GTACGACACT TCATCTGGAC AGACTTAGTT TGGAGACGCC ATCCGAACGT 
ACTTGTTTTT GTATTAAATG TTGGGAAAGT GTTCCCAGTA CCTCTCAAGT TTGGTTCGGT 
GGGAAATGTT GGCATTCCGA TTGTTTTAAA TGTGTGAACT GCAACAAAAA ATTAGACCCT 
TCCTCTGAAG ATTTTTCTCA AGACGATCAA AAGCAAATAT TCTGTAAATT ATGTGTTGAT 
ATCTGCAACG GTTGCTCAAC TCCTATTTGC GAGTTCAACG CAGTAAGCAA TAGTCAAGCT 
AATCATCCTT GTTGCTCCAA TTGTCGAGCT TTCATTAATT CAAATGCTTT TGGCAAGTTC 
AAAGGTCAAC ATTACTGCCT TCCTTGTTTG CGTAAACAAG ATGAAGAGAA TCAAAAGGCT 
GTTATGGAAA GCGCATCAGG GTTTATTCCT CTTAGGAATA TCGCACAAAC TGCGGAAAAC 
ACTTCTTTAA CTGGGCAAAA CCAAGGCTTT CAAAACCCGC ATTTTATAGC GTCTGATTCT 
CCATCCTCCG TATTATCTGG TCGAATGCAA AATACCAGCT CTCCTACAAA CTCTCTCCGT 
CCTCAATTAA AAGTTCAAAC TAATGGTTAT GAAACACCTG GTGATATAAA CTCCGCAAAA 
TCTTACAAAG ACATCCAAAA GGACTTCTTA CTTAAACCCA AAAATTCTTT CGTTAAAACC 
TCTCCCTCTC CACTAGCTAA TGGTCCCTCA TTTCGTTCAG CTAATGCCTC TCCATTTGAT 
TCGTGTGACT CTTTTCATTC TGGAAGTTCG ATACCAATTG AACCAGTTTC TTCAAGGCAA 
AGCTCAGTAG TAAATAATAA TAGCGTGCAG CAACCTGTTG CTTATCACGC CTTTGTTCAA 
TCACCAACCG AAAATGGTAC GCTTCCTCAA CTTCCTAAAA ATGAAAGCGT TGTTAATCCT 
CCTCCACTTC GCCGCTCTTC TACTATGAAT TACAAATCTG TTTCGACAAC AACATCACCA 
TCTAAATATG GATATGTGTC TGGCAGAATA GCTCTATCTC CTATCCATTT AAGGGGTGCT 
CTTCGTGATG TCACAAATAA ATGTAATTTA AAGGTTCCTA GAAATCGAAA CAGTTTATCT 
AATCTTGACG AATATTACGT TAACGGATTG GAAAGCGACG AAACTCCTAC AAAGGCTAGA 
TTTCCTAGAT ATCCTACGGT ATTTAATAAG TTAGATGACA AACGACTTAG TAGCGAACCA 
AACGGCTTGA AGAAAAGATT GACGAACTCT TCAAATTATG AGGCTTCCCC GCGGGCCAAA 
AGTTTAAATT TGTCGCAAGT TAGTTTGCAT CAAGCATGTG AACCTGAATA CAATCGAAGT 
TCTTTGGTTA GAGCAAGTGA TGTGTTTACT TCAAACGTAT TTGATGCTAC AGAGGAAAGC 
GCAAATGAAC TCGCCATTCG TATTTCAGAG CTTCAAGCTG AGGTTGGAAC CTTATTGTTG 
GAAGCGACTT CGTTGGCTTC TATCATAGAG CAGCAGACTC CCGTGTCCCC CGAAGCTTTC 
AAGCAAGAGT TGGTTGCAGA TTTAAATTAT AGATTAGATT ATTTACGGAA ATCTTTTCAA 
CCTGAACTGT CTACCTTACT TTTGAGGCGA GACGCTTTGT CTACGACTGT CTCTAAATTG 
CAAAACGCTT ACAGCACTGT AATGGAAGAA ACCGCATATC TGAATGTTAA GAACACAGAA 
CTGGTAAGCC TTAACAATCA ATTGGAGAGA GAATTGGCAT ACTTGAGGGA GCAGCAGCAT 
AAGAAGCGAA CTTCTTCCTC TTTCGGAATA TTTAATAATG ATAAAAAATC CAACCGAACG 
ATTTCAACCC CGTCTCCGCG AGAATCGTTT TCGCGTCTAC AAATGGTTGC ATCTAGCCTT 
GGATTTAGAC CCAAAGACAA TAAAGACAAG GAGAGTGGAG GGTATAACAA ACGGAACTCT 
AAAATAGATC TTAAGAAGTC ATTTTCTAAA CGGTTTCATT GGAAAAGAGA TTCTCCTCAC 
ATGTCGAGTA CTCCCAGTAT TTCTGAAGAA CATTTAGCTT CTGAAGAACA AGAGGATTTC 
GTTCCAGCGG TCGGTCACTG CAAACTTTGC GGTAAATACT CCAATGAGCT TAGAGCACAT 
TATCAGGATT GCGTTAGTTC TACTATAGAT CGTCAGTATC AATCTAAAAA AAGCGAATCT 
CCTGTTTGGG CTTTGAATCC TGACGACTTC GATCAAAACA GGCTAACACT CTTGAGAGTC 
CCGACGCTTA TCGTTTCTTG TATAAATTTT ATCGAGTCAT ACGGCATGGA TTTTGAAGGT 
CTGTATCGCA AATCAGGGGC AACTTCTCAA ATGAAAAAAA TTGTTGCGTT ACTAAGAAAT 
GAAGATACGG TACTTGATCC AAGTGAGGAT ATTTCTGCTG TCACTTCTGT ATTCAAGCAA 
TATCTTCGCA ACCTTCCTAA TCCAATTATT ACCTATGACC AATACTTTCC GTTCATCACA 
GCAGCAAATT GTGCCTCTTT CCAAGATAAA TTAGACGGTT TTATAATGGT CATCAAAAGT 
CTTCCTCCAG CTCATGCTGA AATTTTGCAG CTCATTATTA GACATTTAGC AAGGGTTGCT 
GCATATAGTC ATGCAAATCG AATGACATCC AAGAACCTTG CAGTAGTATT TTCCCCAACA 
TTAATACGCG ACCCCGATAA TTCCAGAGAC GTTATTGACA TGACTGTTAA GAATTACAGT 
CTTGCGTTTT TGATAGATCA TGTACATGAA GTCTTTGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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rga4 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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