myo51 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	myo51
Entrez Gene ID	2540560
Full Name	myosin type V
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001022153.2	NP_596233.1	myosin type V

Pathways from BioSystems

Homologies

Homo sapiens (human)	MYO5C	NP_061198.2
Pan troglodytes (chimpanzee)	MYO5C	XP_510411.3
Schizosaccharomyces pombe (fission yeast)	myo51	NP_596233.1
Arabidopsis thaliana (thale cress)	XID	NP_180882.2
Xenopus tropicalis (tropical clawed frog)	myo5c	XP_002936392.2
Danio rerio (zebrafish)	myo5c	XP_691143.2
	MYO5C	XP_002804829.1
Canis lupus familiaris (dog)	MYO5C	XP_544680.3
Mus musculus (house mouse)	Myo5c	NP_001074791.1
Rattus norvegicus (Norway rat)	Myo5c	XP_006243475.1
Gallus gallus (chicken)	MYO5C	XP_003641891.1
Arabidopsis thaliana (thale cress)	XIA	NP_171954.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We provide evidence that the myosin II protein Myp2 and the myosin V protein Myo51 play roles in recruiting TORC2 to the CAR.We go on to show that TORC2-dependent phosphorylation of actin-capping protein 1 (Acp1, a known regulator of cytokinesis) controls CAR stability, modulates Acp1-Acp2 heterodimer formation and is essential for survival upon stress.
Title: TOR complex 2 localises to the cytokinetic actomyosin ring and controls the fidelity of cytokinesis.

modified motility assay showed that the additional binding site anchors Myo51-Rng8/9 so that it can cross-link and slide actin-tropomyosin filaments relative to one another, functions that may explain the role of this motor in contractile ring assembly.
Title: A single-headed fission yeast myosin V transports actin in a tropomyosin-dependent manner.

Three myosins function in a hierarchal, complementary manner to accomplish cytokinesis, with Myo2 and Myo51 taking the lead during contractile ring assembly and Myp2 making the greatest contribution to constriction.
Title: Three myosins contribute uniquely to the assembly and constriction of the fission yeast cytokinetic contractile ring.

Rng8 and Rng9 form oligomers and cluster multiple Myo51 dimers to regulate Myo51 localization and functions during fission yeast cytokinesis.
Title: The novel proteins Rng8 and Rng9 regulate the myosin-V Myo51 during fission yeast cytokinesis.

Data show that Myo51 has an important role in regulating spore formation upon completion of meiosis.
Title: Fission yeast Myo51 is a meiotic spindle pole body component with discrete roles during cell fusion and spore formation.

The following myo51 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the myo51 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc100829	NM_001022153.2 Latest version!	Schizosaccharomyces pombe myosin type V (myo51), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

myo51 ( NM_001022153.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_596233.1 Schizosaccharomyces pombe myosin type V (myo51), mRNA.

Price & Availability↑

CloneID	OSc100829
Clone ID Related Accession (Same CDS sequence)	NM_001022153.2
Accession Version	NM_001022153.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	myosin type V
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001022153.1.

ATGAGTCATG CAAGATTATC GGTAGGCTCG GAATGTTGGG TTTCCAATAA TAACGGTCAT 
TGGGATGCTG CTCGATTAAT CGAAATAAAA GATAATGGAG GAGGTAAAGT CGTAGCAACT 
GTTGCAAAGA GCAGCGGTGT TTTAGAAACA GTGAATTACC AACAGTTACA AAATCGAAAC 
ATCGGTCAAT CAGAGTCGCC TTCTGATTTA ACGAATCTTC CTTATTTAAA TGAGCCATCT 
GTCCTTCATG CCTTACATAA TCGTTACAAT AACAAGCAAA TTTACACATA TTCGGGAATC 
GTGCTTGTCT CAATAAACCC TTACCAAAAT CTTCCCGAGT TTTACAACGA CAATCTGATA 
AAACATTTCC ATAAAGATCC CGAAGCAGCC AAAGTTCCTC ACCTATACTC TATAGCAAGT 
TCTTGTTACC ATGCTTTAAC TACAGATTCC AAGAATCAGA CTATTATTGT GTCTGGAGAA 
TCAGGTGCAG GCAAAACAGT TGCTGCTAAG TATATTATGC GTTACTTGAC CTCGGTACAG 
GGAGTAGATC ATAATGGTGT TGTTAAACGC TCTGTTGAGA ATCAAGTTCT CGCTACTAAT 
CCTATTATGG AAGCATTTGG TAACGCAAAA ACTATCAGAA ATGATAACTC TTCACGTTTT 
GGTAAATATG TTACCATAAG CTTTGATGAA AACTTGTTGA TTACCGGAGC AAATGTAAAC 
ACATATTTAC TTGAAAGATC CCGAGTAGTC TCTTTACTTA AAGGGGAAAG AAATTATCAC 
ATTTTTTATC AGCTAATTAC TGGATGTACA GAGGAACAAA GGGATAAATG GTTTCTTGAG 
TCTGCGTCTT CTTTTAATTA TTTATCACAA GGAAATTGTG ATGAAATATC TGGCGTTGAC 
GACAGTAATG ACTTTACTAT AACTTGCAGA GCTCTTTCAA CTATTGGTAT TTCAGAGTCA 
AGGCAGGAGG ATGTATTTTG TCTACTTGCT GCCTTACTCC ACCTTGGTAA CATTGAAGTC 
TGTGCAACAA GAAATGAAGC ACAAATACAG CCCGGTGATG GGTATCTTCA GAAAGCTGCT 
TTATTACTTG GTGTCGATTC ATCTACGCTT GCTAAATGGA TTGTTAAAAG ACAACTGAAA 
ACTAGATCCG AAACCATTAT TACCTCATCA ACTTTGGAAC ATGCTATTAG CATAAGAGAC 
TCTGTCGCTA AATATTTATA TTCCGCACTG TTTTTGTGGA TTGTTCACAT GATTAATGCA 
TCACTTGACC ATAACAAAGT TAAAAGAGCT GCCTACAAAT ATATTGGTGT TGTTGATATT 
TATGGCTTCG AACATTTCGA AAAAAATTCG ATGGAGCAAT TCTGTATTAA TTATGCTAAT 
GAAAAACTTC AACAAGAATT TAATAAGCAC GTTTTTAAAC TAGAACAGGA AGAATATGTT 
AAAGAAGGAT TAGACTGGCG ACTTATAGAA TATTCTGACA ATCAAGGTTG CATATCCTTA 
ATCGAAGACA AATTAGGGAT ACTCTCGTTG TTGGATGAGG AATGTCGCTT ACCTTCAGGG 
AACCACCAAT CGTTTTTACA AAAATTAAAT AATCAACTTC CTACAAAACA CTCTCAGTTC 
TATAAAAAAT CTAGATTTAA TGATGGTTCA TTTATGGTTA AACATTATGC TTTGGACGTT 
AGTTATCAAG TACACGATTT TTTAGCAAAA AATTCGGATG CCATACCTGA TGAGTTTATA 
AGTTTACTAC AAAACTCAAA AAATGAGTTT ATAACTTATC TGCTAGACTT TTATATGCAG 
TTGGTTTCTT CTCAAAATAA AAATCCTAGA AAGACTGCTA TAAGCAGGAA ACCTACACTT 
TCATCAATGT TTAAATCTTC TTTGTCTCAA TTAATGACAA CTGTTTCCTC TACTAATGTT 
CATTACATTC GATGCATTAA GCCTAATGAA GAAAAGCTTC CTTGGACGTT CTCCCCGCCG 
ATGGTGCTCT CACAACTCAG GGCCTGCGGT GTTTTTGAAA CAATTAGGAT ATCCTCGTTA 
GGGTTTCCTG CTCGATTTTC GTATGAGGAA TTTGCTCATC GATTCCGCAT TTTGTTGTCT 
TCTAAAGAAT GGGAGGAAGA TAATAAAAAG TTGACTCTTA ATATTGTTAA CTCTGTAATT 
CCTCATGATA ACCTAAACTT TCAAGTTGGT CGCTCCAAAA TATTTTTCAG ATCAAACGTT 
ATTGGAAATT TTGAAGAAGC CCATCGAGCT ACATGTAGCA AATCGACAGT TTTATTACAG 
TCTGCTATAA GAGGCTTCTT TACTCGGAAG GAGTATCAAA GAACTGTAAA ATTTATCATT 
AAACTACAAT CGGTTATTAT GGGCTGGTTA ACTCGTCAAC GTTTTGAGCG AGAAAAAATT 
GAACGAGCAG CCATTCTTAT TCAAGCTCAT TGGCGATCTT ATATCCAACG CAAAAGATAT 
TTGTCCCTTA TCAAATGTGC TATTGTTATT CAATCAATAG TTCGGAAGAA TATCGCATAT 
TCTCGTTATA TAAATGAGTT AAGGGAAAGT TCTGCTACAC TTCTTGCAAA ATTTTGGCGA 
GCGTACAACG CAAGAAAAAC TTTTAGAGGT TTGAAGAAGT CAGTTATTGC TCTGCAATGT 
GTGTCGCGCT CTGTATTAAC CAGAAGATAT CTTCGCAGAT TACAAGATTC GGCGGGCAGG 
ACTAGCATTC TTTATGAGAA GCAAAAGAAC CTGCAGGCTT CTATAACCGA AGTATCCAAA 
CAGTTGAAAT CAAATAGCAA AAAAGTAACA GTTTTAAGGA ATAAGCTTAA TATTTTAAAT 
AACTCACTAT CGAAATGGAA GTGTTTAATT AAGAAGCCCA GTGACTTTTC TGAACCCGTT 
AGTATGGATT TTACTAGCAA CGATGAACAG CTTGTGCAAC TTTTGCAAGC CGAATCAAAG 
CTTCGGCAAG CATCTCAGCA ACTTTATATG GCAGCCAAAA AATCGGAGTT GGGATTTGTT 
CAATCTCAAA CTGCCCGAGA GAACTTGTCG AATTATTACC AAGCTTTACA GATGACAGTT 
TCTGAAAAAT TTGAATATGA TACTGAACAA CTACCTTCCC GTGTTCTTTT TTACGCAATG 
GATAGATACT TCTCCATCCA CAAAAAACTC AAACAGCTTC TTGAATTAGT TGGTGTTGAA 
AATGCATCTC TCTTACCTAA TGAAGTTGTT AATAAACAAA CTAAAGATTT ATTGTATGAG 
AAAAGGGTTG TTTTTTTAAA GCAAATTAAG CAAGCTCTCA CGGTTTCTTC TTTGTTTAAT 
GCAGTTGGTT ACAAAGATGG TGTTATGAGG TTGTTAGAAA CGGATCAGAA CTCACTACTT 
TTTGCTGGGG TAGTAAACTT TCTAATTTTT GCAGGTATTA GCCTTGATTT GAAGACACAA 
ATCAGTGAGT TTTTGAGCCA ATTGTGTTCC TATTTCACTA AAATCGTTGA CGGTACAGTT 
ATTGAAAATG ATAAAACTCT AGATTTCTAT GAAAAACCTC TACAAGCGGT TTTGTATTGG 
TTCGCAACGT TACACAAAAT TCGTTCATTT TTAGTGCATC TTTTATCTAT TAATTCACAT 
GGAAAGCAAA GTGTGGTAGA GGATTTATGG AATCCGTTGA TTTTGAAATT CTCAAAACAT 
TTTTCTAATC TGGAAAATTC ATTTCATTCC TTGGTCCAGA AACTATTAAG TTGTTGTACC 
GAGGGATCGA TTAATGCTCT TTTAAATTCA AAATGTTTAC CAGAGTTTAT CGATGCGGCT 
GATGAGAACA CAACACCTAC TGGAATGAAT ATTTATGAGT TGATAGACAG AATGAATTTA 
ATACACAAAT TATTAATATC TTCTGCCTTG CAACCAAATT TATTGGAATT GACTATATCT 
CACATGCTTC AACATATAGG ACAACGTGCC TTTCAAACAT TAATTCATGG CCGGTCACCG 
TACACTTGGA AATCTGCTTC TCAGGTTTCC TATAATGCCT CGCTGTTGAT TAACTGGTGT 
CATCAAAAGG GTATTAGTTA TGTTAACTCG TCACTATTGC CTTTAATGCA GTCTCCTTTG 
GTATTTTGCC TTCGTAAAAA CGATGCAAAC GATTTGGATG TTATTCTCTC TGTTTGTAAT 
TTACTTTCGC CATTTGAAGT TGTATGCCTC CTAAATAGGT ATCAGCCATG CGCTGGTGAA 
AATCCTCTAC CAAAATCCTT TTCAAAAGCC GTAGAGGCAT TATCTTGTAA GTATAAGCAA 
TCTGGTTTTA CAAATGGTAA AATCACAAAC ACTAATGGGC ACGCTATTCC TATAGCTGCT 
TCTAAAAACC CTTTATTATC ACTTGAAAAT AATCATATAT ACGAAGAGCT TCGACTTTCA 
GAGTTGATAA ACTTATTGGC TAAAGCTACA TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_596233.1
CDS	116..4531
Translation	MSHARLSVGSECWVSNNNGHWDAARLIEIKDNGGGKVVATVAKSSGVLETVNYQQLQNRNIGQSESPSDLTNLPYLNEPSVLHALHNRYNNKQIYTYSGIVLVSINPYQNLPEFYNDNLIKHFHKDPEAAKVPHLYSIASSCYHALTTDSKNQTIIVSGESGAGKTVAAKYIMRYLTSVQGVDHNGVVKRSVENQVLATNPIMEAFGNAKTIRNDNSSRFGKYVTISFDENLLITGANVNTYLLERSRVVSLLKGERNYHIFYQLITGCTEEQRDKWFLESASSFNYLSQGNCDEISGVDDSNDFTITCRALSTIGISESRQEDVFCLLAALLHLGNIEVCATRNEAQIQPGDGYLQKAALLLGVDSSTLAKWIVKRQLKTRSETIITSSTLEHAISIRDSVAKYLYSALFLWIVHMINASLDHNKVKRAAYKYIGVVDIYGFEHFEKNSMEQFCINYANEKLQQEFNKHVFKLEQEEYVKEGLDWRLIEYSDNQGCISLIEDKLGILSLLDEECRLPSGNHQSFLQKLNNQLPTKHSQFYKKSRFNDGSFMVKHYALDVSYQVHDFLAKNSDAIPDEFISLLQNSKNEFITYLLDFYMQLVSSQNKNPRKTAISRKPTLSSMFKSSLSQLMTTVSSTNVHYIRCIKPNEEKLPWTFSPPMVLSQLRACGVFETIRISSLGFPARFSYEEFAHRFRILLSSKEWEEDNKKLTLNIVNSVIPHDNLNFQVGRSKIFFRSNVIGNFEEAHRATCSKSTVLLQSAIRGFFTRKEYQRTVKFIIKLQSVIMGWLTRQRFEREKIERAAILIQAHWRSYIQRKRYLSLIKCAIVIQSIVRKNIAYSRYINELRESSATLLAKFWRAYNARKTFRGLKKSVIALQCVSRSVLTRRYLRRLQDSAGRTSILYEKQKNLQASITEVSKQLKSNSKKVTVLRNKLNILNNSLSKWKCLIKKPSDFSEPVSMDFTSNDEQLVQLLQAESKLRQASQQLYMAAKKSELGFVQSQTARENLSNYYQALQMTVSEKFEYDTEQLPSRVLFYAMDRYFSIHKKLKQLLELVGVENASLLPNEVVNKQTKDLLYEKRVVFLKQIKQALTVSSLFNAVGYKDGVMRLLETDQNSLLFAGVVNFLIFAGISLDLKTQISEFLSQLCSYFTKIVDGTVIENDKTLDFYEKPLQAVLYWFATLHKIRSFLVHLLSINSHGKQSVVEDLWNPLILKFSKHFSNLENSFHSLVQKLLSCCTEGSINALLNSKCLPEFIDAADENTTPTGMNIYELIDRMNLIHKLLISSALQPNLLELTISHMLQHIGQRAFQTLIHGRSPYTWKSASQVSYNASLLINWCHQKGISYVNSSLLPLMQSPLVFCLRKNDANDLDVILSVCNLLSPFEVVCLLNRYQPCAGENPLPKSFSKAVEALSCKYKQSGFTNGKITNTNGHAIPIAASKNPLLSLENNHIYEELRLSELINLLAKATL

Target ORF information:

RefSeq Version	NM_001022153.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe myosin type V (myo51), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001022153.2

ORF Insert Sequence:

ATGAGTCATG CAAGATTATC GGTAGGCTCG GAATGTTGGG TTTCCAATAA TAACGGTCAT 
TGGGATGCTG CTCGATTAAT CGAAATAAAA GATAATGGAG GAGGTAAAGT CGTAGCAACT 
GTTGCAAAGA GCAGCGGTGT TTTAGAAACA GTGAATTACC AACAGTTACA AAATCGAAAC 
ATCGGTCAAT CAGAGTCGCC TTCTGATTTA ACGAATCTTC CTTATTTAAA TGAGCCATCT 
GTCCTTCATG CCTTACATAA TCGTTACAAT AACAAGCAAA TTTACACATA TTCGGGAATC 
GTGCTTGTCT CAATAAACCC TTACCAAAAT CTTCCCGAGT TTTACAACGA CAATCTGATA 
AAACATTTCC ATAAAGATCC CGAAGCAGCC AAAGTTCCTC ACCTATACTC TATAGCAAGT 
TCTTGTTACC ATGCTTTAAC TACAGATTCC AAGAATCAGA CTATTATTGT GTCTGGAGAA 
TCAGGTGCAG GCAAAACAGT TGCTGCTAAG TATATTATGC GTTACTTGAC CTCGGTACAG 
GGAGTAGATC ATAATGGTGT TGTTAAACGC TCTGTTGAGA ATCAAGTTCT CGCTACTAAT 
CCTATTATGG AAGCATTTGG TAACGCAAAA ACTATCAGAA ATGATAACTC TTCACGTTTT 
GGTAAATATG TTACCATAAG CTTTGATGAA AACTTGTTGA TTACCGGAGC AAATGTAAAC 
ACATATTTAC TTGAAAGATC CCGAGTAGTC TCTTTACTTA AAGGGGAAAG AAATTATCAC 
ATTTTTTATC AGCTAATTAC TGGATGTACA GAGGAACAAA GGGATAAATG GTTTCTTGAG 
TCTGCGTCTT CTTTTAATTA TTTATCACAA GGAAATTGTG ATGAAATATC TGGCGTTGAC 
GACAGTAATG ACTTTACTAT AACTTGCAGA GCTCTTTCAA CTATTGGTAT TTCAGAGTCA 
AGGCAGGAGG ATGTATTTTG TCTACTTGCT GCCTTACTCC ACCTTGGTAA CATTGAAGTC 
TGTGCAACAA GAAATGAAGC ACAAATACAG CCCGGTGATG GGTATCTTCA GAAAGCTGCT 
TTATTACTTG GTGTCGATTC ATCTACGCTT GCTAAATGGA TTGTTAAAAG ACAACTGAAA 
ACTAGATCCG AAACCATTAT TACCTCATCA ACTTTGGAAC ATGCTATTAG CATAAGAGAC 
TCTGTCGCTA AATATTTATA TTCCGCACTG TTTTTGTGGA TTGTTCACAT GATTAATGCA 
TCACTTGACC ATAACAAAGT TAAAAGAGCT GCCTACAAAT ATATTGGTGT TGTTGATATT 
TATGGCTTCG AACATTTCGA AAAAAATTCG ATGGAGCAAT TCTGTATTAA TTATGCTAAT 
GAAAAACTTC AACAAGAATT TAATAAGCAC GTTTTTAAAC TAGAACAGGA AGAATATGTT 
AAAGAAGGAT TAGACTGGCG ACTTATAGAA TATTCTGACA ATCAAGGTTG CATATCCTTA 
ATCGAAGACA AATTAGGGAT ACTCTCGTTG TTGGATGAGG AATGTCGCTT ACCTTCAGGG 
AACCACCAAT CGTTTTTACA AAAATTAAAT AATCAACTTC CTACAAAACA CTCTCAGTTC 
TATAAAAAAT CTAGATTTAA TGATGGTTCA TTTATGGTTA AACATTATGC TTTGGACGTT 
AGTTATCAAG TACACGATTT TTTAGCAAAA AATTCGGATG CCATACCTGA TGAGTTTATA 
AGTTTACTAC AAAACTCAAA AAATGAGTTT ATAACTTATC TGCTAGACTT TTATATGCAG 
TTGGTTTCTT CTCAAAATAA AAATCCTAGA AAGACTGCTA TAAGCAGGAA ACCTACACTT 
TCATCAATGT TTAAATCTTC TTTGTCTCAA TTAATGACAA CTGTTTCCTC TACTAATGTT 
CATTACATTC GATGCATTAA GCCTAATGAA GAAAAGCTTC CTTGGACGTT CTCCCCGCCG 
ATGGTGCTCT CACAACTCAG GGCCTGCGGT GTTTTTGAAA CAATTAGGAT ATCCTCGTTA 
GGGTTTCCTG CTCGATTTTC GTATGAGGAA TTTGCTCATC GATTCCGCAT TTTGTTGTCT 
TCTAAAGAAT GGGAGGAAGA TAATAAAAAG TTGACTCTTA ATATTGTTAA CTCTGTAATT 
CCTCATGATA ACCTAAACTT TCAAGTTGGT CGCTCCAAAA TATTTTTCAG ATCAAACGTT 
ATTGGAAATT TTGAAGAAGC CCATCGAGCT ACATGTAGCA AATCGACAGT TTTATTACAG 
TCTGCTATAA GAGGCTTCTT TACTCGGAAG GAGTATCAAA GAACTGTAAA ATTTATCATT 
AAACTACAAT CGGTTATTAT GGGCTGGTTA ACTCGTCAAC GTTTTGAGCG AGAAAAAATT 
GAACGAGCAG CCATTCTTAT TCAAGCTCAT TGGCGATCTT ATATCCAACG CAAAAGATAT 
TTGTCCCTTA TCAAATGTGC TATTGTTATT CAATCAATAG TTCGGAAGAA TATCGCATAT 
TCTCGTTATA TAAATGAGTT AAGGGAAAGT TCTGCTACAC TTCTTGCAAA ATTTTGGCGA 
GCGTACAACG CAAGAAAAAC TTTTAGAGGT TTGAAGAAGT CAGTTATTGC TCTGCAATGT 
GTGTCGCGCT CTGTATTAAC CAGAAGATAT CTTCGCAGAT TACAAGATTC GGCGGGCAGG 
ACTAGCATTC TTTATGAGAA GCAAAAGAAC CTGCAGGCTT CTATAACCGA AGTATCCAAA 
CAGTTGAAAT CAAATAGCAA AAAAGTAACA GTTTTAAGGA ATAAGCTTAA TATTTTAAAT 
AACTCACTAT CGAAATGGAA GTGTTTAATT AAGAAGCCCA GTGACTTTTC TGAACCCGTT 
AGTATGGATT TTACTAGCAA CGATGAACAG CTTGTGCAAC TTTTGCAAGC CGAATCAAAG 
CTTCGGCAAG CATCTCAGCA ACTTTATATG GCAGCCAAAA AATCGGAGTT GGGATTTGTT 
CAATCTCAAA CTGCCCGAGA GAACTTGTCG AATTATTACC AAGCTTTACA GATGACAGTT 
TCTGAAAAAT TTGAATATGA TACTGAACAA CTACCTTCCC GTGTTCTTTT TTACGCAATG 
GATAGATACT TCTCCATCCA CAAAAAACTC AAACAGCTTC TTGAATTAGT TGGTGTTGAA 
AATGCATCTC TCTTACCTAA TGAAGTTGTT AATAAACAAA CTAAAGATTT ATTGTATGAG 
AAAAGGGTTG TTTTTTTAAA GCAAATTAAG CAAGCTCTCA CGGTTTCTTC TTTGTTTAAT 
GCAGTTGGTT ACAAAGATGG TGTTATGAGG TTGTTAGAAA CGGATCAGAA CTCACTACTT 
TTTGCTGGGG TAGTAAACTT TCTAATTTTT GCAGGTATTA GCCTTGATTT GAAGACACAA 
ATCAGTGAGT TTTTGAGCCA ATTGTGTTCC TATTTCACTA AAATCGTTGA CGGTACAGTT 
ATTGAAAATG ATAAAACTCT AGATTTCTAT GAAAAACCTC TACAAGCGGT TTTGTATTGG 
TTCGCAACGT TACACAAAAT TCGTTCATTT TTAGTGCATC TTTTATCTAT TAATTCACAT 
GGAAAGCAAA GTGTGGTAGA GGATTTATGG AATCCGTTGA TTTTGAAATT CTCAAAACAT 
TTTTCTAATC TGGAAAATTC ATTTCATTCC TTGGTCCAGA AACTATTAAG TTGTTGTACC 
GAGGGATCGA TTAATGCTCT TTTAAATTCA AAATGTTTAC CAGAGTTTAT CGATGCGGCT 
GATGAGAACA CAACACCTAC TGGAATGAAT ATTTATGAGT TGATAGACAG AATGAATTTA 
ATACACAAAT TATTAATATC TTCTGCCTTG CAACCAAATT TATTGGAATT GACTATATCT 
CACATGCTTC AACATATAGG ACAACGTGCC TTTCAAACAT TAATTCATGG CCGGTCACCG 
TACACTTGGA AATCTGCTTC TCAGGTTTCC TATAATGCCT CGCTGTTGAT TAACTGGTGT 
CATCAAAAGG GTATTAGTTA TGTTAACTCG TCACTATTGC CTTTAATGCA GTCTCCTTTG 
GTATTTTGCC TTCGTAAAAA CGATGCAAAC GATTTGGATG TTATTCTCTC TGTTTGTAAT 
TTACTTTCGC CATTTGAAGT TGTATGCCTC CTAAATAGGT ATCAGCCATG CGCTGGTGAA 
AATCCTCTAC CAAAATCCTT TTCAAAAGCC GTAGAGGCAT TATCTTGTAA GTATAAGCAA 
TCTGGTTTTA CAAATGGTAA AATCACAAAC ACTAATGGGC ACGCTATTCC TATAGCTGCT 
TCTAAAAACC CTTTATTATC ACTTGAAAAT AATCATATAT ACGAAGAGCT TCGACTTTCA 
GAGTTGATAA ACTTATTGGC TAAAGCTACA TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
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myo51 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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