SPBC27B12.05 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following SPBC27B12.05 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPBC27B12.05 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc99978 NM_001021448.2
Latest version!
Schizosaccharomyces pombe WD repeat protein (SPBC27B12.05), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc99978
    Clone ID Related Accession (Same CDS sequence) NM_001021448.2
    Accession Version NM_001021448.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1449bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product WD repeat protein
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021448.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGCAAAGAG ACCTAACAGT TGATGAATTC CTAAACTATC TATATATTAA TGTTAAAGAA 
    TTATTCACAT TGAAAGAGAA ATCCTTTCAT TTTTGGAGAA ATTCACTGAT TTCATGTAGT
    AAAACGAAGA ATTTTCTGTT TGTGGTTGTA GGCGATGAAG TTCTTGTTTA TGACTTAAAG
    GAGATCTCAT CAGATGCTTC AGAACCTGAA CCTTGCTTTT ATTTGCATTG TCCTCATGAA
    GCATCTTCCT CGCGGCAAGG AGATATCAAT CAAGACATGC CAAATGTCAT CAACAATGTT
    CGTTGTGAAT TAATTGGAGA TTTGGAGTAT TTTATTACCG CCAATGATTG TGGAAAAGTA
    GCGATATACG ATGTTCAAAA GTTAAATCGA GAGCCTATAA TTTATGATGT TCAGCATTCA
    GCTTGGGGAC TAGCAGTTTC ACCTCTTAGG ATCCTTGGCA TATCTTCCAA TTCTCACAAT
    GTTAATTTAT TTCATCTGTC GCCTGAATTT AAACGATTTA AAGAGGATTA CCATTCTCCT
    TGGTTTAGAC AAGAGACATT AGTACTGGAA GGACATGAAC ATAATATACC GTGTATAACA
    TTTAATTCTT CAGGAACATT ATTACTGAGT GGATCTATTG ATCGATCACT TCAGATATGG
    GATATAACTT CATTAAGTTG CCTTTGCAAG TTTTATACAA AGTTAAGTAT TTGGGGAGTT
    AAGTTCATTG ATAAAAATGC GTTCTATCAT ATTTCCATCC TAAAGCACAA ACCGGGATCT
    CTTATTCCGG CGCAACAATT TGGCCAAAGC CCTTGGAAGC CACCCATCAT GTCCAGTTCA
    GCTTTCTTAT TATCGCATGA AGAAGATTTC GATGACGACG CGGTTTTTGA AGGTGAATAC
    TATGTTCATA TACAGGATGA ACTTCCGAAG ACGAAGGTAA AAAATTATTT TCGTACTTAT
    CTGCGGCAGA AACCTGAAGA ACTATTCATT TTTACTACGA AATTTTCAAT AGTTTTATGT
    GCTTACATGG GAAATTCACA AATATTTCCT TTGGTGAGCA GTAAAAATTG CTTTGAGGAA
    CCCATTACCC CCAGGTTTCA AGCTTTACAT AGAATCAATA TGATAGAATG TATTCCCGAA
    TTACAGTCAG TTGTTTGTGC CAGTCAGAGT GGTCAATTAA CATTGCTTCG CTTGATTTGT
    ACAACAAAAA TTCTCAATGG AAATCCAATT TATGTTTACT CATTTGTTCC CCATAAAATT
    CGCCTCACTC ATTTGGTGGA TGCTCCTTTA TTGGGAATGA GTGTTTGCCC ATTGTATCCT
    GGAGAAGAAA ATCCTTTTAA AAGATTTAAA ATAATGCTTA CTGTTTATAC CGGAAAAGTT
    ATTTCTGTAG AAGTTCAGCT CTCGGAAAAT TTATACGACA CTAGTCATAT AGGAAACATA
    CCTCTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_595537.2
    CDS36..1484
    Translation

    Target ORF information:

    RefSeq Version NM_001021448.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe WD repeat protein (SPBC27B12.05), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001021448.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGCAAAGAG ACCTAACAGT TGATGAATTC CTAAACTATC TATATATTAA TGTTAAAGAA 
    TTATTCACAT TGAAAGAGAA ATCCTTTCAT TTTTGGAGAA ATTCACTGAT TTCATGTAGT
    AAAACGAAGA ATTTTCTGTT TGTGGTTGTA GGCGATGAAG TTCTTGTTTA TGACTTAAAG
    GAGATCTCAT CAGATGCTTC AGAACCTGAA CCTTGCTTTT ATTTGCATTG TCCTCATGAA
    GCATCTTCCT CGCGGCAAGG AGATATCAAT CAAGACATGC CAAATGTCAT CAACAATGTT
    CGTTGTGAAT TAATTGGAGA TTTGGAGTAT TTTATTACCG CCAATGATTG TGGAAAAGTA
    GCGATATACG ATGTTCAAAA GTTAAATCGA GAGCCTATAA TTTATGATGT TCAGCATTCA
    GCTTGGGGAC TAGCAGTTTC ACCTCTTAGG ATCCTTGGCA TATCTTCCAA TTCTCACAAT
    GTTAATTTAT TTCATCTGTC GCCTGAATTT AAACGATTTA AAGAGGATTA CCATTCTCCT
    TGGTTTAGAC AAGAGACATT AGTACTGGAA GGACATGAAC ATAATATACC GTGTATAACA
    TTTAATTCTT CAGGAACATT ATTACTGAGT GGATCTATTG ATCGATCACT TCAGATATGG
    GATATAACTT CATTAAGTTG CCTTTGCAAG TTTTATACAA AGTTAAGTAT TTGGGGAGTT
    AAGTTCATTG ATAAAAATGC GTTCTATCAT ATTTCCATCC TAAAGCACAA ACCGGGATCT
    CTTATTCCGG CGCAACAATT TGGCCAAAGC CCTTGGAAGC CACCCATCAT GTCCAGTTCA
    GCTTTCTTAT TATCGCATGA AGAAGATTTC GATGACGACG CGGTTTTTGA AGGTGAATAC
    TATGTTCATA TACAGGATGA ACTTCCGAAG ACGAAGGTAA AAAATTATTT TCGTACTTAT
    CTGCGGCAGA AACCTGAAGA ACTATTCATT TTTACTACGA AATTTTCAAT AGTTTTATGT
    GCTTACATGG GAAATTCACA AATATTTCCT TTGGTGAGCA GTAAAAATTG CTTTGAGGAA
    CCCATTACCC CCAGGTTTCA AGCTTTACAT AGAATCAATA TGATAGAATG TATTCCCGAA
    TTACAGTCAG TTGTTTGTGC CAGTCAGAGT GGTCAATTAA CATTGCTTCG CTTGATTTGT
    ACAACAAAAA TTCTCAATGG AAATCCAATT TATGTTTACT CATTTGTTCC CCATAAAATT
    CGCCTCACTC ATTTGGTGGA TGCTCCTTTA TTGGGAATGA GTGTTTGCCC ATTGTATCCT
    GGAGAAGAAA ATCCTTTTAA AAGATTTAAA ATAATGCTTA CTGTTTATAC CGGAAAAGTT
    ATTTCTGTAG AAGTTCAGCT CTCGGAAAAT TTATACGACA CTAGTCATAT AGGAAACATA
    CCTCTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L