blt1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	blt1
Entrez Gene ID	2540380
Full Name	Blt1
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001021710.2	NP_595807.1	Blt1
NM_001356242.1	NP_001342740.1	Blt1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Blt1p ensures proper timing for onset of contractile ring constriction during cytokinesis.
Title: Characterization of the roles of Blt1p in fission yeast cytokinesis.

Blt1 acts as a scaffolding protein for precursors of the cytokinetic ring, and Blt1 and Mid1 provide overlapping membrane anchors for proper division plane positioning.
Title: Blt1 and Mid1 provide overlapping membrane anchors to position the division plane in fission yeast.

The following blt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the blt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSj01675	NM_001021710.2 Latest version!	Schizosaccharomyces pombe 972h- Blt1 (blt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OSc100714	NM_001356242.1 Latest version!	Schizosaccharomyces pombe Blt1 (blt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

blt1 ( NM_001021710.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_595807.1 Schizosaccharomyces pombe 972h- Blt1 (blt1), mRNA.

Price & Availability↑

CloneID	OSj01675
Clone ID Related Accession (Same CDS sequence)	NM_001021710.2
Accession Version	NM_001021710.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-30
Organism	Schizosaccharomyces pombe 972h-
Product	Blt1
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021710.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

ATGAGCAAAT CTGCTTTCAC AAGCAAAAGT CAGCTTAAAG GAATAGATGG AAGTTTTAAT 
GACCCATTTC GGCAACCGTC TATGAATTTG CCTACTCCCC CTCATGATGA TGGTCTTCCA 
TCAATACCTA GGTCTACAGC CTTGCCTAAT TTGTCTAACC GATTATCTCC ATATTCGCAT 
CGTAATTACC TACCAATTCC TGAAGCGGAT TCTCGTAATT TAGAGGTGCT TAATTCAATT 
TCGCTCACAA CAGGATCAGT CGTGAATCAA ATAAATAAAC TTTACCAGAG GAGTTGTGAT 
AATGCTAATT ACTTACAAGA CTTACGCTCT TTAATACTTC AAACTCCCAC GGCTGAAGCA 
AATGCCACCC AAGTTACCGA ATCTCTAAAC CAAATCCAAA AAATTTTACA AGAGGCTTCT 
TCTAAAGATA GAGAACAAAT TGTTCAAGTT ATTAAAGAAC TCAATAAAGG TAACAGTTTG 
GAGGTTCGTA GAGAACTTGG AAATTGTTTG GCCCATCTTA AGAAAGGAAC TTTAAATATA 
GACAACGCAT TACAAGCCTC TCTACAAAAA TACTGGAAAG AATTGCAGTA CTTTAATGTG 
TCAAAGGATA AGTTGCTATC TTTAGAGGAA AGTATAAAAT TGTTAAGTGC AAGATTAGAG 
GAAACTGATA CACCCTCCTC CACTCATTGG ATAGAGATAA ATGCTGGATT TAAAGAAGTT 
GGTGACGAAT TGAGCGAGAA TTTTCAAAGA AAGCAAGAAA TTTTGAGTGG ATTACAAAAT 
AATGTGATTC TTCGTACTAA TAATAGGAAG CCTGTAGGCA GTGATGGCTC TAATTCTAAT 
TTTAATGGTG GAGAAGAACA GATGGACTCC AAAGATCAAG AGGAAATACA AGATTATTTG 
AATTCTCTTT TGTCAGTTCA TGAGAAAGAT GGTGTACTTC TCCAGAAATT TCATAATTCT 
TATGTTCAAT ATCAGAAACT CGCTGTTGCA TTGTCGAAAT ATGAGAATTT CGATGCTGAT 
AAGCTGTTTC AACAGATGAA TTTAGCCAAG CAAAGCAATG AGACTCTTTT ACAAACTTTG 
ACCGAACAAA CCGATCAACT GCTTTCGTTC AAGGAAACCA TGGACTCATT TAAATTTATT 
TTAGGTCCCT CTATGGAGAA CCAGGCTTTA CAGCAGGGAA TTCTAGCAGA GCAGCAAGAT 
TTGGTAGCCC AGTTACGCGA TATTGTGCTT CGCTCAGAGA CGCTAGCTGA AAATCCCTCT 
AATATGCCCG GTAGTTGCCT TCCTGGAGCC TCCTCAAATA CTGCTGACGA GTTTACTGAA 
CAGCTGAATC TACTTAAGAA CGAAGTAGCA CGCCTCTCTG CAATCTGTCC AAGCCCTAAT 
TCTGGAATTA ATGCTTCTGT GCTTACTAAT GCTGACAATT TAGAGAAAGA GAACCTCCTG 
TTAGTGAACA ACAATGCCTT CAAAGTAGAT GATCGTTCAG TGTCATCAGT TGCATTAGAC 
GATCATAATC GGCAGTTACA AATGAACGTA GAGGAGTTAG AAGGAAAGAA GGCGGATTTA 
ACCAGCAAAA TTAATCGATT GGATAAGGAC TTTGTTAAGC TAAATACAAC CTACTCGTTA 
CTCACCGATC AAGTCAAAAC TAAGCAATTG GCACTCCAAG AGATTGAAAG TCGAGTTATT 
CGTCTGGAAG AAAGACTAAA TATGTTACAG AAGCTGTCCA TGCAGCCAGC TATTTCTTCG 
AGCAGCGAAT TTGTTCCCAT TGAATCCCAT CCCTCATCTT CAGCTGTTGT ACCGATAGAA 
GAACCAAAAA ATAGTATTAT AGAAAAGAAG CGTGTTCCAC ATAACGCTGC AAGAAATTCT 
GTTAATTTGG AGGTCACTCT TCCTAATTCT AAAAAGAGGT TTTCTTCGTT TTCTGGATCT 
TCCAGTAAAC TACCAGTCCG TCCATCAACT GCGTTAACAG ACAAAAGGAA ACCATCTTGG 
TCAAGAAGGC TAGCTGCTGC AATCGGGTTT TCTTCAGGAT CTCCAGAAAA GAAGCATGTC 
ATAACTTCAT CGGACGCGGG ACATCAGCGA TCTAAATCAA GAAGCTTCAG TTCTAAAATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_595807.1
CDS	105..2207
Translation	MSKSAFTSKSQLKGIDGSFNDPFRQPSMNLPTPPHDDGLPSIPRSTALPNLSNRLSPYSHRNYLPIPEADSRNLEVLNSISLTTGSVVNQINKLYQRSCDNANYLQDLRSLILQTPTAEANATQVTESLNQIQKILQEASSKDREQIVQVIKELNKGNSLEVRRELGNCLAHLKKGTLNIDNALQASLQKYWKELQYFNVSKDKLLSLEESIKLLSARLEETDTPSSTHWIEINAGFKEVGDELSENFQRKQEILSGLQNNVILRTNNRKPVGSDGSNSNFNGGEEQMDSKDQEEIQDYLNSLLSVHEKDGVLLQKFHNSYVQYQKLAVALSKYENFDADKLFQQMNLAKQSNETLLQTLTEQTDQLLSFKETMDSFKFILGPSMENQALQQGILAEQQDLVAQLRDIVLRSETLAENPSNMPGSCLPGASSNTADEFTEQLNLLKNEVARLSAICPSPNSGINASVLTNADNLEKENLLLVNNNAFKVDDRSVSSVALDDHNRQLQMNVEELEGKKADLTSKINRLDKDFVKLNTTYSLLTDQVKTKQLALQEIESRVIRLEERLNMLQKLSMQPAISSSSEFVPIESHPSSSAVVPIEEPKNSIIEKKRVPHNAARNSVNLEVTLPNSKKRFSSFSGSSSKLPVRPSTALTDKRKPSWSRRLAAAIGFSSGSPEKKHVITSSDAGHQRSKSRSFSSKM

Target ORF information:

RefSeq Version	NM_001021710.2
Organism	Schizosaccharomyces pombe 972h-
Definition	Schizosaccharomyces pombe 972h- Blt1 (blt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001021710.2

ORF Insert Sequence:

ATGAGCAAAT CTGCTTTCAC AAGCAAAAGT CAGCTTAAAG GAATAGATGG AAGTTTTAAT 
GACCCATTTC GGCAACCGTC TATGAATTTG CCTACTCCCC CTCATGATGA TGGTCTTCCA 
TCAATACCTA GGTCTACAGC CTTGCCTAAT TTGTCTAACC GATTATCTCC ATATTCGCAT 
CGTAATTACC TACCAATTCC TGAAGCGGAT TCTCGTAATT TAGAGGTGCT TAATTCAATT 
TCGCTCACAA CAGGATCAGT CGTGAATCAA ATAAATAAAC TTTACCAGAG GAGTTGTGAT 
AATGCTAATT ACTTACAAGA CTTACGCTCT TTAATACTTC AAACTCCCAC GGCTGAAGCA 
AATGCCACCC AAGTTACCGA ATCTCTAAAC CAAATCCAAA AAATTTTACA AGAGGCTTCT 
TCTAAAGATA GAGAACAAAT TGTTCAAGTT ATTAAAGAAC TCAATAAAGG TAACAGTTTG 
GAGGTTCGTA GAGAACTTGG AAATTGTTTG GCCCATCTTA AGAAAGGAAC TTTAAATATA 
GACAACGCAT TACAAGCCTC TCTACAAAAA TACTGGAAAG AATTGCAGTA CTTTAATGTG 
TCAAAGGATA AGTTGCTATC TTTAGAGGAA AGTATAAAAT TGTTAAGTGC AAGATTAGAG 
GAAACTGATA CACCCTCCTC CACTCATTGG ATAGAGATAA ATGCTGGATT TAAAGAAGTT 
GGTGACGAAT TGAGCGAGAA TTTTCAAAGA AAGCAAGAAA TTTTGAGTGG ATTACAAAAT 
AATGTGATTC TTCGTACTAA TAATAGGAAG CCTGTAGGCA GTGATGGCTC TAATTCTAAT 
TTTAATGGTG GAGAAGAACA GATGGACTCC AAAGATCAAG AGGAAATACA AGATTATTTG 
AATTCTCTTT TGTCAGTTCA TGAGAAAGAT GGTGTACTTC TCCAGAAATT TCATAATTCT 
TATGTTCAAT ATCAGAAACT CGCTGTTGCA TTGTCGAAAT ATGAGAATTT CGATGCTGAT 
AAGCTGTTTC AACAGATGAA TTTAGCCAAG CAAAGCAATG AGACTCTTTT ACAAACTTTG 
ACCGAACAAA CCGATCAACT GCTTTCGTTC AAGGAAACCA TGGACTCATT TAAATTTATT 
TTAGGTCCCT CTATGGAGAA CCAGGCTTTA CAGCAGGGAA TTCTAGCAGA GCAGCAAGAT 
TTGGTAGCCC AGTTACGCGA TATTGTGCTT CGCTCAGAGA CGCTAGCTGA AAATCCCTCT 
AATATGCCCG GTAGTTGCCT TCCTGGAGCC TCCTCAAATA CTGCTGACGA GTTTACTGAA 
CAGCTGAATC TACTTAAGAA CGAAGTAGCA CGCCTCTCTG CAATCTGTCC AAGCCCTAAT 
TCTGGAATTA ATGCTTCTGT GCTTACTAAT GCTGACAATT TAGAGAAAGA GAACCTCCTG 
TTAGTGAACA ACAATGCCTT CAAAGTAGAT GATCGTTCAG TGTCATCAGT TGCATTAGAC 
GATCATAATC GGCAGTTACA AATGAACGTA GAGGAGTTAG AAGGAAAGAA GGCGGATTTA 
ACCAGCAAAA TTAATCGATT GGATAAGGAC TTTGTTAAGC TAAATACAAC CTACTCGTTA 
CTCACCGATC AAGTCAAAAC TAAGCAATTG GCACTCCAAG AGATTGAAAG TCGAGTTATT 
CGTCTGGAAG AAAGACTAAA TATGTTACAG AAGCTGTCCA TGCAGCCAGC TATTTCTTCG 
AGCAGCGAAT TTGTTCCCAT TGAATCCCAT CCCTCATCTT CAGCTGTTGT ACCGATAGAA 
GAACCAAAAA ATAGTATTAT AGAAAAGAAG CGTGTTCCAC ATAACGCTGC AAGAAATTCT 
GTTAATTTGG AGGTCACTCT TCCTAATTCT AAAAAGAGGT TTTCTTCGTT TTCTGGATCT 
TCCAGTAAAC TACCAGTCCG TCCATCAACT GCGTTAACAG ACAAAAGGAA ACCATCTTGG 
TCAAGAAGGC TAGCTGCTGC AATCGGGTTT TCTTCAGGAT CTCCAGAAAA GAAGCATGTC 
ATAACTTCAT CGGACGCGGG ACATCAGCGA TCTAAATCAA GAAGCTTCAG TTCTAAAATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

blt1 ( NM_001356242.1 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_001342740.1 Schizosaccharomyces pombe Blt1 (blt1), mRNA.

Price & Availability↑

CloneID	OSc100714
Clone ID Related Accession (Same CDS sequence)	NM_001356242.1
Accession Version	NM_001356242.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	Blt1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423).

ATGAGCAAAT CTGCTTTCAC AAGCAAAAGT CAGCTTAAAG GAATAGATGG AAGTTTTAAT 
GACCCATTTC GGCAACCGTC TATGAATTTG CCTACTCCCC CTCATGATGA TGGTCTTCCA 
TCAATACCTA GGTCTACAGC CTTGCCTAAT TTGTCTAACC GATTATCTCC ATATTCGCAT 
CGTAATTACC TACCAATTCC TGAAGCGGAT TCTCGTAATT TAGAGGTGCT TAATTCAATT 
TCGCTCACAA CAGGATCAGT CGTGAATCAA ATAAATAAAC TTTACCAGAG GAGTTGTGAT 
AATGCTAATT ACTTACAAGA CTTACGCTCT TTAATACTTC AAACTCCCAC GGCTGAAGCA 
AATGCCACCC AAGTTACCGA ATCTCTAAAC CAAATCCAAA AAATTTTACA AGAGGCTTCT 
TCTAAAGATA GAGAACAAAT TGTTCAAGTT ATTAAAGAAC TCAATAAAGG TAACAGTTTG 
GAGGTTCGTA GAGAACTTGG AAATTGTTTG GCCCATCTTA AGAAAGGAAC TTTAAATATA 
GACAACGCAT TACAAGCCTC TCTACAAAAA TACTGGAAAG AATTGCAGTA CTTTAATGTG 
TCAAAGGATA AGTTGCTATC TTTAGAGGAA AGTATAAAAT TGTTAAGTGC AAGATTAGAG 
GAAACTGATA CACCCTCCTC CACTCATTGG ATAGAGATAA ATGCTGGATT TAAAGAAGTT 
GGTGACGAAT TGAGCGAGAA TTTTCAAAGA AAGCAAGAAA TTTTGAGTGG ATTACAAAAT 
AATGTGATTC TTCGTACTAA TAATAGGAAG CCTGTAGGCA GTGATGGCTC TAATTCTAAT 
TTTAATGGTG GAGAAGAACA GATGGACTCC AAAGATCAAG AGGAAATACA AGATTATTTG 
AATTCTCTTT TGTCAGTTCA TGAGAAAGAT GGTGTACTTC TCCAGAAATT TCATAATTCT 
TATGTTCAAT ATCAGAAACT CGCTGTTGCA TTGTCGAAAT ATGAGAATTT CGATGCTGAT 
AAGCTGTTTC AACAGATGAA TTTAGCCAAG CAAAGCAATG AGACTCTTTT ACAAACTTTG 
ACCGAACAAA CCGATCAACT GCTTTCGTTC AAGGAAACCA TGGACTCATT TAAATTTATT 
TTAGGTCCCT CTATGGAGAA CCAGGCTTTA CAGCAGGGAA TTCTAGCAGA GCAGCAAGAT 
TTGGTAGCCC AGTTACGCGA TATTGTGCTT CGCTCAGAGA CGCTAGCTGA AAATCCCTCT 
AATATGCCCG GTAGTTGCCT TCCTGGAGCC TCCTCAAATA CTGCTGACGA GTTTACTGAA 
CAGCTGAATC TACTTAAGAA CGAAGTAGCA CGCCTCTCTG CAATCTGTCC AAGCCCTAAT 
TCTGGAATTA ATGCTTCTGT GCTTACTAAT GCTGACAATT TAGAGAAAGA GAACCTCCTG 
TTAGTGAACA ACAATGCCTT CAAAGTAGAT GATCGTTCAG TGTCATCAGT TGCATTAGAC 
GATCATAATC GGCAGTTACA AATGAACGTA GAGGAGTTAG AAGGAAAGAA GGCGGATTTA 
ACCAGCAAAA TTAATCGATT GGATAAGGAC TTTGTTAAGC TAAATACAAC CTACTCGTTA 
CTCACCGATC AAGTCAAAAC TAAGCAATTG GCACTCCAAG AGATTGAAAG TCGAGTTATT 
CGTCTGGAAG AAAGACTAAA TATGTTACAG AAGCTGTCCA TGCAGCCAGC TATTTCTTCG 
AGCAGCGAAT TTGTTCCCAT TGAATCCCAT CCCTCATCTT CAGCTGTTGT ACCGATAGAA 
GAACCAAAAA ATAGTATTAT AGAAAAGAAG CGTGTTCCAC ATAACGCTGC AAGAAATTCT 
GTTAATTTGG AGGTCACTCT TCCTAATTCT AAAAAGAGGT TTTCTTCGTT TTCTGGATCT 
TCCAGTAAAC TACCAGTCCG TCCATCAACT GCGTTAACAG ACAAAAGGAA ACCATCTTGG 
TCAAGAAGGC TAGCTGCTGC AATCGGGTTT TCTTCAGGAT CTCCAGAAAA GAAGCATGTC 
ATAACTTCAT CGGACGCGGG ACATCAGCGA TCTAAATCAA GAAGCTTCAG TTCTAAAATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342740.1
CDS	105..2207
Translation	MSKSAFTSKSQLKGIDGSFNDPFRQPSMNLPTPPHDDGLPSIPRSTALPNLSNRLSPYSHRNYLPIPEADSRNLEVLNSISLTTGSVVNQINKLYQRSCDNANYLQDLRSLILQTPTAEANATQVTESLNQIQKILQEASSKDREQIVQVIKELNKGNSLEVRRELGNCLAHLKKGTLNIDNALQASLQKYWKELQYFNVSKDKLLSLEESIKLLSARLEETDTPSSTHWIEINAGFKEVGDELSENFQRKQEILSGLQNNVILRTNNRKPVGSDGSNSNFNGGEEQMDSKDQEEIQDYLNSLLSVHEKDGVLLQKFHNSYVQYQKLAVALSKYENFDADKLFQQMNLAKQSNETLLQTLTEQTDQLLSFKETMDSFKFILGPSMENQALQQGILAEQQDLVAQLRDIVLRSETLAENPSNMPGSCLPGASSNTADEFTEQLNLLKNEVARLSAICPSPNSGINASVLTNADNLEKENLLLVNNNAFKVDDRSVSSVALDDHNRQLQMNVEELEGKKADLTSKINRLDKDFVKLNTTYSLLTDQVKTKQLALQEIESRVIRLEERLNMLQKLSMQPAISSSSEFVPIESHPSSSAVVPIEEPKNSIIEKKRVPHNAARNSVNLEVTLPNSKKRFSSFSGSSSKLPVRPSTALTDKRKPSWSRRLAAAIGFSSGSPEKKHVITSSDAGHQRSKSRSFSSKM

Target ORF information:

RefSeq Version	NM_001356242.1
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe Blt1 (blt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356242.1

ORF Insert Sequence:

ATGAGCAAAT CTGCTTTCAC AAGCAAAAGT CAGCTTAAAG GAATAGATGG AAGTTTTAAT 
GACCCATTTC GGCAACCGTC TATGAATTTG CCTACTCCCC CTCATGATGA TGGTCTTCCA 
TCAATACCTA GGTCTACAGC CTTGCCTAAT TTGTCTAACC GATTATCTCC ATATTCGCAT 
CGTAATTACC TACCAATTCC TGAAGCGGAT TCTCGTAATT TAGAGGTGCT TAATTCAATT 
TCGCTCACAA CAGGATCAGT CGTGAATCAA ATAAATAAAC TTTACCAGAG GAGTTGTGAT 
AATGCTAATT ACTTACAAGA CTTACGCTCT TTAATACTTC AAACTCCCAC GGCTGAAGCA 
AATGCCACCC AAGTTACCGA ATCTCTAAAC CAAATCCAAA AAATTTTACA AGAGGCTTCT 
TCTAAAGATA GAGAACAAAT TGTTCAAGTT ATTAAAGAAC TCAATAAAGG TAACAGTTTG 
GAGGTTCGTA GAGAACTTGG AAATTGTTTG GCCCATCTTA AGAAAGGAAC TTTAAATATA 
GACAACGCAT TACAAGCCTC TCTACAAAAA TACTGGAAAG AATTGCAGTA CTTTAATGTG 
TCAAAGGATA AGTTGCTATC TTTAGAGGAA AGTATAAAAT TGTTAAGTGC AAGATTAGAG 
GAAACTGATA CACCCTCCTC CACTCATTGG ATAGAGATAA ATGCTGGATT TAAAGAAGTT 
GGTGACGAAT TGAGCGAGAA TTTTCAAAGA AAGCAAGAAA TTTTGAGTGG ATTACAAAAT 
AATGTGATTC TTCGTACTAA TAATAGGAAG CCTGTAGGCA GTGATGGCTC TAATTCTAAT 
TTTAATGGTG GAGAAGAACA GATGGACTCC AAAGATCAAG AGGAAATACA AGATTATTTG 
AATTCTCTTT TGTCAGTTCA TGAGAAAGAT GGTGTACTTC TCCAGAAATT TCATAATTCT 
TATGTTCAAT ATCAGAAACT CGCTGTTGCA TTGTCGAAAT ATGAGAATTT CGATGCTGAT 
AAGCTGTTTC AACAGATGAA TTTAGCCAAG CAAAGCAATG AGACTCTTTT ACAAACTTTG 
ACCGAACAAA CCGATCAACT GCTTTCGTTC AAGGAAACCA TGGACTCATT TAAATTTATT 
TTAGGTCCCT CTATGGAGAA CCAGGCTTTA CAGCAGGGAA TTCTAGCAGA GCAGCAAGAT 
TTGGTAGCCC AGTTACGCGA TATTGTGCTT CGCTCAGAGA CGCTAGCTGA AAATCCCTCT 
AATATGCCCG GTAGTTGCCT TCCTGGAGCC TCCTCAAATA CTGCTGACGA GTTTACTGAA 
CAGCTGAATC TACTTAAGAA CGAAGTAGCA CGCCTCTCTG CAATCTGTCC AAGCCCTAAT 
TCTGGAATTA ATGCTTCTGT GCTTACTAAT GCTGACAATT TAGAGAAAGA GAACCTCCTG 
TTAGTGAACA ACAATGCCTT CAAAGTAGAT GATCGTTCAG TGTCATCAGT TGCATTAGAC 
GATCATAATC GGCAGTTACA AATGAACGTA GAGGAGTTAG AAGGAAAGAA GGCGGATTTA 
ACCAGCAAAA TTAATCGATT GGATAAGGAC TTTGTTAAGC TAAATACAAC CTACTCGTTA 
CTCACCGATC AAGTCAAAAC TAAGCAATTG GCACTCCAAG AGATTGAAAG TCGAGTTATT 
CGTCTGGAAG AAAGACTAAA TATGTTACAG AAGCTGTCCA TGCAGCCAGC TATTTCTTCG 
AGCAGCGAAT TTGTTCCCAT TGAATCCCAT CCCTCATCTT CAGCTGTTGT ACCGATAGAA 
GAACCAAAAA ATAGTATTAT AGAAAAGAAG CGTGTTCCAC ATAACGCTGC AAGAAATTCT 
GTTAATTTGG AGGTCACTCT TCCTAATTCT AAAAAGAGGT TTTCTTCGTT TTCTGGATCT 
TCCAGTAAAC TACCAGTCCG TCCATCAACT GCGTTAACAG ACAAAAGGAA ACCATCTTGG 
TCAAGAAGGC TAGCTGCTGC AATCGGGTTT TCTTCAGGAT CTCCAGAAAA GAAGCATGTC 
ATAACTTCAT CGGACGCGGG ACATCAGCGA TCTAAATCAA GAAGCTTCAG TTCTAAAATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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blt1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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