LOC109712878 cDNA ORF clone, Ananas comosus(pineapple)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109712878
Entrez Gene ID	109712878
Full Name	switch 2
Gene Type	protein-coding
Organism	Ananas comosus(pineapple)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020236671.1	XP_020092260.1	switch 2 isoform X1
XM_020236672.1	XP_020092261.1	switch 2 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC109712878 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109712878 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAn67878	XM_020236671.1 Latest version!	Ananas comosus switch 2 (LOC109712878), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OAn67879	XM_020236672.1 Latest version!	Ananas comosus switch 2 (LOC109712878), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109712878 ( XM_020236671.1 ) cDNA ORF clone, Ananas comosus(pineapple) -> XP_020092260.1 Ananas comosus switch 2 (LOC109712878), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAn67878
Clone ID Related Accession (Same CDS sequence)	XM_020236671.1
Accession Version	XM_020236671.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Ananas comosus(pineapple)
Product	switch 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033627.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ananas comosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCTAA ACCGCCTCAA AGAAACCCTC CGATTCTCCT CCACCACCAC CACCACCTCC 
TCCTCCTCCG CCGCATCTTC TTTCTCCCTC TCCGAACCCC TTCCTCCTCT GAGGAAACCC 
CCCAAATCCT CGCTCTCTCA GCAGCTCCAA CGCCTCCACC TCCCCTCCTC CTCCTCCTCC 
TCCTCTTCCT CCTCCGATCC ACCTCCGCTT CCTCCGCCTC CGCCTCCGCC TCCGCCTCCG 
CCCCCACGAC GACGAGAGGA AGAAGAAGGC GACAAAGACG AGGACGAGGA CGAGGACGAC 
GATGGTGAAA GGGACGGTGC ACTATCTCGC CCTAATCGGG AGCCCGTTCT TTCTCAGCCT 
TTCTCTTTCG AGGTGCGGGG GCCTTACGAG CCCTTGGTCC TCTCGTCCCC CCGGGAGGAT 
CCGATCGTGC AGGTTCCTGC ATCAATCAAT TGTAGGCTTC TTGAACATCA AAGAGATGGC 
GTGAGGTTCT TGTATAATTT GTACAAGAAC AACCATGGTG GCGTACTTGG GGATGACATG 
GGCTTGGGGA AGACCATCCA AACAATTGCC TTTTTGGCTG CTGTGATGGG AAAAGACCCT 
GAACAAGGAG ACCATTTGAA AGACACGAAA GACCATTATA TGAATAAAGG TCCAGTACTT 
ATCCTCTGCC CCACTACTGT AATTCGCAAC TGGGAAAACG AGTTTTCTGA ATGGGGGGAT 
TTCACAGTCG CTGTTTATCA CGGACCTAAT CGGGAATTAA TACTAGATAA AATTGAGGCA 
CAATGCGTGG AGATACTCAT AACTAGTTTT GACACTTTCA GGATTCATGA CAAAATTTTA 
TCTGAGATCT CATGGGAAAT TGTGATTGTT GATGAAGCTC ATCGTTTAAA GAATGAAAAA 
TCTAAACTCT ATACAGCATG TTTAGGAATA AGAACAAGCA AAAGGTATGG ACTCACAGGG 
ACTATAATGC AGAACAAGAT ATTAGAATTA TTTAATCTGT TTGACTGGGT TGCACCTGGG 
TCTTTGGGAG ATAGAGAGCA TTTTCGAGCT TTTTATGATG AGCCATTAAA GCAAGGTCAG 
AGGTTCAGTG CTCCTGAAAG ATTTATTCAA ATTGCTGATG AGCGCAAAAA GCACCTTGTT 
GCAGTTCTAA GAAAATATTT ATTAAGAAGG ACAAAGGAGG AGACCATAGG GCATCTGATG 
CTTGGAAAAG AAGATAACGT TGTTTTCTGC AAAATGAGTG ACCTACAGAA GCGTGTTTAC 
AGGAGATTAT TGCAACAACC TGATATACAG TGCCTTGTAA ATAAAGATCT TCCTTGTAGC 
TGTGGAAGTC CTCTAACTCA GGTGGAGTGT TGTAAAAAAA TTGAGCCTAA GGGCATTATT 
TGGTCTTATC TCCATAAGGA CAACCCCGAT GGTTGTGCTT TGTGCCCTTT CTGCCTCGTT 
CTTCCTTGCC TAGTCAAGCT TCAGCAGATT AGCAATCACC TGGAGCTGAT AAAGCCTAGC 
CCTAAGGATG AAAGTGAGAA ACAAAAGAAG GATTCTGAAC TAGCAGCTGC TGTCTTTGGT 
GATGATATTG GCTTGGTTGG CGGTGGTGCT AAAAGTGAAA GTTTCATGGG CCTTAGTGAT 
GCAGAACATT GTGGGAAAAT GCGAGCATTG GAAAAGCTGT TGGCATCATG GACTTCGCGT 
GGTGACAAGA TACTTCTATT CAGCTACTCT GTCAGGATGC TTGACATACT GGAGAAGTTC 
CTAATACGGA AAGGCTATTG CTTTTCCCGG TTCGACGGTT CAACTCCCAT GAATGCACGT 
CAGTCACTTG TTGATGAGTT CAACAGAAGT CCAAGTAAAC AGGTTTTCCT AATATCAACT 
CGGGCAGGTA ATCTAGGCGT GAATCTTGTG AGTGCTAACC GTGTCGTAAT ATTCGATCCG 
AGTTGGAATC CAGCACAAGA TTTGCAGGCT CAAGACAGAT CGTTTCGCTA TGGACAGAAA 
CGGCATGTTG TAGTATTTCG TTTACTTGCT GCTGGTTCGT TTGAAGAACT TGTTTATTCA 
CGGCAAGTGT ATAAACAGCA GCTTTCCAAT ATCGCTGTTT CGGGGAAAGT GGAAAAGCGT 
TACTTTGAAG GTGTTCAGGA CTGCAAGAAG TTTCAAGGAG AGTTATTTGG AATTTGCAAT 
CTGTTCCGAG ACCTGTCTGA CAAGCTTTTT ACAAGTGAGA TCATTGAAAT GCATGAGGAG 
CAAGGGAGGA ATACTGGAAC AGATAATGTG CCAGTAAGCC TGAAAATTGA TGTTAAATCT 
AATGCTAATG AAGCCAATAA GGTATCAAAC TTACTGAAGT CTTATGGGGA AGCAACTCGT 
GCTGAAAATG AGGCAACACT GGATGATTTG GGCATAGTGT ATGCCCATCG CAACGAAGAC 
GTCGTTAACA TGAAAATGGG AGGAAATCAT GGTGGAATAA GTACCCATGA AGATGATACC 
AACAAGACTT TTCCTACCAA GGCAGCAGAT AGAAGCCATG AAAGGAAACA TGTAAAGTTG 
AGAGGAGGAG AACCTTCGTT AGCCCATCAA AAAAGAAGAG AGTTTAGTCG AATTGCTCGA 
TACATGAATA TGGGGGAGCT CGAGTTCAGT AAGTGGTTAC TCTCTGCTTC TTATTTAGAG 
AGGATGGAAA TGCTACAGAA CTACAAGAAA AAGAAGAAGA AGAAGAAGAA GAAGAAGAAA 
CAGAAGGTTG AAGTTGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020092260.1
CDS	39..2759
Translation	MSLNRLKETLRFSSTTTTTSSSSAASSFSLSEPLPPLRKPPKSSLSQQLQRLHLPSSSSSSSSSSDPPPLPPPPPPPPPPPPRRREEEEGDKDEDEDEDDDGERDGALSRPNREPVLSQPFSFEVRGPYEPLVLSSPREDPIVQVPASINCRLLEHQRDGVRFLYNLYKNNHGGVLGDDMGLGKTIQTIAFLAAVMGKDPEQGDHLKDTKDHYMNKGPVLILCPTTVIRNWENEFSEWGDFTVAVYHGPNRELILDKIEAQCVEILITSFDTFRIHDKILSEISWEIVIVDEAHRLKNEKSKLYTACLGIRTSKRYGLTGTIMQNKILELFNLFDWVAPGSLGDREHFRAFYDEPLKQGQRFSAPERFIQIADERKKHLVAVLRKYLLRRTKEETIGHLMLGKEDNVVFCKMSDLQKRVYRRLLQQPDIQCLVNKDLPCSCGSPLTQVECCKKIEPKGIIWSYLHKDNPDGCALCPFCLVLPCLVKLQQISNHLELIKPSPKDESEKQKKDSELAAAVFGDDIGLVGGGAKSESFMGLSDAEHCGKMRALEKLLASWTSRGDKILLFSYSVRMLDILEKFLIRKGYCFSRFDGSTPMNARQSLVDEFNRSPSKQVFLISTRAGNLGVNLVSANRVVIFDPSWNPAQDLQAQDRSFRYGQKRHVVVFRLLAAGSFEELVYSRQVYKQQLSNIAVSGKVEKRYFEGVQDCKKFQGELFGICNLFRDLSDKLFTSEIIEMHEEQGRNTGTDNVPVSLKIDVKSNANEANKVSNLLKSYGEATRAENEATLDDLGIVYAHRNEDVVNMKMGGNHGGISTHEDDTNKTFPTKAADRSHERKHVKLRGGEPSLAHQKRREFSRIARYMNMGELEFSKWLLSASYLERMEMLQNYKKKKKKKKKKKKQKVEVD

Target ORF information:

RefSeq Version	XM_020236671.1
Organism	Ananas comosus(pineapple)
Definition	Ananas comosus switch 2 (LOC109712878), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020236671.1

ORF Insert Sequence:

ATGTCCCTAA ACCGCCTCAA AGAAACCCTC CGATTCTCCT CCACCACCAC CACCACCTCC 
TCCTCCTCCG CCGCATCTTC TTTCTCCCTC TCCGAACCCC TTCCTCCTCT GAGGAAACCC 
CCCAAATCCT CGCTCTCTCA GCAGCTCCAA CGCCTCCACC TCCCCTCCTC CTCCTCCTCC 
TCCTCTTCCT CCTCCGATCC ACCTCCGCTT CCTCCGCCTC CGCCTCCGCC TCCGCCTCCG 
CCCCCACGAC GACGAGAGGA AGAAGAAGGC GACAAAGACG AGGACGAGGA CGAGGACGAC 
GATGGTGAAA GGGACGGTGC ACTATCTCGC CCTAATCGGG AGCCCGTTCT TTCTCAGCCT 
TTCTCTTTCG AGGTGCGGGG GCCTTACGAG CCCTTGGTCC TCTCGTCCCC CCGGGAGGAT 
CCGATCGTGC AGGTTCCTGC ATCAATCAAT TGTAGGCTTC TTGAACATCA AAGAGATGGC 
GTGAGGTTCT TGTATAATTT GTACAAGAAC AACCATGGTG GCGTACTTGG GGATGACATG 
GGCTTGGGGA AGACCATCCA AACAATTGCC TTTTTGGCTG CTGTGATGGG AAAAGACCCT 
GAACAAGGAG ACCATTTGAA AGACACGAAA GACCATTATA TGAATAAAGG TCCAGTACTT 
ATCCTCTGCC CCACTACTGT AATTCGCAAC TGGGAAAACG AGTTTTCTGA ATGGGGGGAT 
TTCACAGTCG CTGTTTATCA CGGACCTAAT CGGGAATTAA TACTAGATAA AATTGAGGCA 
CAATGCGTGG AGATACTCAT AACTAGTTTT GACACTTTCA GGATTCATGA CAAAATTTTA 
TCTGAGATCT CATGGGAAAT TGTGATTGTT GATGAAGCTC ATCGTTTAAA GAATGAAAAA 
TCTAAACTCT ATACAGCATG TTTAGGAATA AGAACAAGCA AAAGGTATGG ACTCACAGGG 
ACTATAATGC AGAACAAGAT ATTAGAATTA TTTAATCTGT TTGACTGGGT TGCACCTGGG 
TCTTTGGGAG ATAGAGAGCA TTTTCGAGCT TTTTATGATG AGCCATTAAA GCAAGGTCAG 
AGGTTCAGTG CTCCTGAAAG ATTTATTCAA ATTGCTGATG AGCGCAAAAA GCACCTTGTT 
GCAGTTCTAA GAAAATATTT ATTAAGAAGG ACAAAGGAGG AGACCATAGG GCATCTGATG 
CTTGGAAAAG AAGATAACGT TGTTTTCTGC AAAATGAGTG ACCTACAGAA GCGTGTTTAC 
AGGAGATTAT TGCAACAACC TGATATACAG TGCCTTGTAA ATAAAGATCT TCCTTGTAGC 
TGTGGAAGTC CTCTAACTCA GGTGGAGTGT TGTAAAAAAA TTGAGCCTAA GGGCATTATT 
TGGTCTTATC TCCATAAGGA CAACCCCGAT GGTTGTGCTT TGTGCCCTTT CTGCCTCGTT 
CTTCCTTGCC TAGTCAAGCT TCAGCAGATT AGCAATCACC TGGAGCTGAT AAAGCCTAGC 
CCTAAGGATG AAAGTGAGAA ACAAAAGAAG GATTCTGAAC TAGCAGCTGC TGTCTTTGGT 
GATGATATTG GCTTGGTTGG CGGTGGTGCT AAAAGTGAAA GTTTCATGGG CCTTAGTGAT 
GCAGAACATT GTGGGAAAAT GCGAGCATTG GAAAAGCTGT TGGCATCATG GACTTCGCGT 
GGTGACAAGA TACTTCTATT CAGCTACTCT GTCAGGATGC TTGACATACT GGAGAAGTTC 
CTAATACGGA AAGGCTATTG CTTTTCCCGG TTCGACGGTT CAACTCCCAT GAATGCACGT 
CAGTCACTTG TTGATGAGTT CAACAGAAGT CCAAGTAAAC AGGTTTTCCT AATATCAACT 
CGGGCAGGTA ATCTAGGCGT GAATCTTGTG AGTGCTAACC GTGTCGTAAT ATTCGATCCG 
AGTTGGAATC CAGCACAAGA TTTGCAGGCT CAAGACAGAT CGTTTCGCTA TGGACAGAAA 
CGGCATGTTG TAGTATTTCG TTTACTTGCT GCTGGTTCGT TTGAAGAACT TGTTTATTCA 
CGGCAAGTGT ATAAACAGCA GCTTTCCAAT ATCGCTGTTT CGGGGAAAGT GGAAAAGCGT 
TACTTTGAAG GTGTTCAGGA CTGCAAGAAG TTTCAAGGAG AGTTATTTGG AATTTGCAAT 
CTGTTCCGAG ACCTGTCTGA CAAGCTTTTT ACAAGTGAGA TCATTGAAAT GCATGAGGAG 
CAAGGGAGGA ATACTGGAAC AGATAATGTG CCAGTAAGCC TGAAAATTGA TGTTAAATCT 
AATGCTAATG AAGCCAATAA GGTATCAAAC TTACTGAAGT CTTATGGGGA AGCAACTCGT 
GCTGAAAATG AGGCAACACT GGATGATTTG GGCATAGTGT ATGCCCATCG CAACGAAGAC 
GTCGTTAACA TGAAAATGGG AGGAAATCAT GGTGGAATAA GTACCCATGA AGATGATACC 
AACAAGACTT TTCCTACCAA GGCAGCAGAT AGAAGCCATG AAAGGAAACA TGTAAAGTTG 
AGAGGAGGAG AACCTTCGTT AGCCCATCAA AAAAGAAGAG AGTTTAGTCG AATTGCTCGA 
TACATGAATA TGGGGGAGCT CGAGTTCAGT AAGTGGTTAC TCTCTGCTTC TTATTTAGAG 
AGGATGGAAA TGCTACAGAA CTACAAGAAA AAGAAGAAGA AGAAGAAGAA GAAGAAGAAA 
CAGAAGGTTG AAGTTGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109712878 ( XM_020236672.1 ) cDNA ORF clone, Ananas comosus(pineapple) -> XP_020092261.1 Ananas comosus switch 2 (LOC109712878), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAn67879
Clone ID Related Accession (Same CDS sequence)	XM_020236672.1
Accession Version	XM_020236672.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Ananas comosus(pineapple)
Product	switch 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033627.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ananas comosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCTAA ACCGCCTCAA AGAAACCCTC CGATTCTCCT CCACCACCAC CACCACCTCC 
TCCTCCTCCG CCGCATCTTC TTTCTCCCTC TCCGAACCCC TTCCTCCTCT GAGGAAACCC 
CCCAAATCCT CGCTCTCTCA GCAGCTCCAA CGCCTCCACC TCCCCTCCTC CTCCTCCTCC 
TCCTCTTCCT CCTCCGATCC ACCTCCGCTT CCTCCGCCTC CGCCTCCGCC TCCGCCTCCG 
CCCCCACGAC GACGAGAGGA AGAAGAAGGC GACAAAGACG AGGACGAGGA CGAGGACGAC 
GATGGTGAAA GGGACGGTGC ACTATCTCGC CCTAATCGGG AGCCCGTTCT TTCTCAGCCT 
TTCTCTTTCG AGGTGCGGGG GCCTTACGAG CCCTTGGTCC TCTCGTCCCC CCGGGAGGAT 
CCGATCGTGC AGGTTCCTGC ATCAATCAAT TGTAGGCTTC TTGAACATCA AAGAGATGGC 
GTGAGGTTCT TGTATAATTT GTACAAGAAC AACCATGGTG GCGTACTTGG GGATGACATG 
ATTCATGACA AAATTTTATC TGAGATCTCA TGGGAAATTG TGATTGTTGA TGAAGCTCAT 
CGTTTAAAGA ATGAAAAATC TAAACTCTAT ACAGCATGTT TAGGAATAAG AACAAGCAAA 
AGGTATGGAC TCACAGGGAC TATAATGCAG AACAAGATAT TAGAATTATT TAATCTGTTT 
GACTGGGTTG CACCTGGGTC TTTGGGAGAT AGAGAGCATT TTCGAGCTTT TTATGATGAG 
CCATTAAAGC AAGGTCAGAG GTTCAGTGCT CCTGAAAGAT TTATTCAAAT TGCTGATGAG 
CGCAAAAAGC ACCTTGTTGC AGTTCTAAGA AAATATTTAT TAAGAAGGAC AAAGGAGGAG 
ACCATAGGGC ATCTGATGCT TGGAAAAGAA GATAACGTTG TTTTCTGCAA AATGAGTGAC 
CTACAGAAGC GTGTTTACAG GAGATTATTG CAACAACCTG ATATACAGTG CCTTGTAAAT 
AAAGATCTTC CTTGTAGCTG TGGAAGTCCT CTAACTCAGG TGGAGTGTTG TAAAAAAATT 
GAGCCTAAGG GCATTATTTG GTCTTATCTC CATAAGGACA ACCCCGATGG TTGTGCTTTG 
TGCCCTTTCT GCCTCGTTCT TCCTTGCCTA GTCAAGCTTC AGCAGATTAG CAATCACCTG 
GAGCTGATAA AGCCTAGCCC TAAGGATGAA AGTGAGAAAC AAAAGAAGGA TTCTGAACTA 
GCAGCTGCTG TCTTTGGTGA TGATATTGGC TTGGTTGGCG GTGGTGCTAA AAGTGAAAGT 
TTCATGGGCC TTAGTGATGC AGAACATTGT GGGAAAATGC GAGCATTGGA AAAGCTGTTG 
GCATCATGGA CTTCGCGTGG TGACAAGATA CTTCTATTCA GCTACTCTGT CAGGATGCTT 
GACATACTGG AGAAGTTCCT AATACGGAAA GGCTATTGCT TTTCCCGGTT CGACGGTTCA 
ACTCCCATGA ATGCACGTCA GTCACTTGTT GATGAGTTCA ACAGAAGTCC AAGTAAACAG 
GTTTTCCTAA TATCAACTCG GGCAGGTAAT CTAGGCGTGA ATCTTGTGAG TGCTAACCGT 
GTCGTAATAT TCGATCCGAG TTGGAATCCA GCACAAGATT TGCAGGCTCA AGACAGATCG 
TTTCGCTATG GACAGAAACG GCATGTTGTA GTATTTCGTT TACTTGCTGC TGGTTCGTTT 
GAAGAACTTG TTTATTCACG GCAAGTGTAT AAACAGCAGC TTTCCAATAT CGCTGTTTCG 
GGGAAAGTGG AAAAGCGTTA CTTTGAAGGT GTTCAGGACT GCAAGAAGTT TCAAGGAGAG 
TTATTTGGAA TTTGCAATCT GTTCCGAGAC CTGTCTGACA AGCTTTTTAC AAGTGAGATC 
ATTGAAATGC ATGAGGAGCA AGGGAGGAAT ACTGGAACAG ATAATGTGCC AGTAAGCCTG 
AAAATTGATG TTAAATCTAA TGCTAATGAA GCCAATAAGG TATCAAACTT ACTGAAGTCT 
TATGGGGAAG CAACTCGTGC TGAAAATGAG GCAACACTGG ATGATTTGGG CATAGTGTAT 
GCCCATCGCA ACGAAGACGT CGTTAACATG AAAATGGGAG GAAATCATGG TGGAATAAGT 
ACCCATGAAG ATGATACCAA CAAGACTTTT CCTACCAAGG CAGCAGATAG AAGCCATGAA 
AGGAAACATG TAAAGTTGAG AGGAGGAGAA CCTTCGTTAG CCCATCAAAA AAGAAGAGAG 
TTTAGTCGAA TTGCTCGATA CATGAATATG GGGGAGCTCG AGTTCAGTAA GTGGTTACTC 
TCTGCTTCTT ATTTAGAGAG GATGGAAATG CTACAGAACT ACAAGAAAAA GAAGAAGAAG 
AAGAAGAAGA AGAAGAAACA GAAGGTTGAA GTTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020092261.1
CDS	39..2477
Translation	MSLNRLKETLRFSSTTTTTSSSSAASSFSLSEPLPPLRKPPKSSLSQQLQRLHLPSSSSSSSSSSDPPPLPPPPPPPPPPPPRRREEEEGDKDEDEDEDDDGERDGALSRPNREPVLSQPFSFEVRGPYEPLVLSSPREDPIVQVPASINCRLLEHQRDGVRFLYNLYKNNHGGVLGDDMIHDKILSEISWEIVIVDEAHRLKNEKSKLYTACLGIRTSKRYGLTGTIMQNKILELFNLFDWVAPGSLGDREHFRAFYDEPLKQGQRFSAPERFIQIADERKKHLVAVLRKYLLRRTKEETIGHLMLGKEDNVVFCKMSDLQKRVYRRLLQQPDIQCLVNKDLPCSCGSPLTQVECCKKIEPKGIIWSYLHKDNPDGCALCPFCLVLPCLVKLQQISNHLELIKPSPKDESEKQKKDSELAAAVFGDDIGLVGGGAKSESFMGLSDAEHCGKMRALEKLLASWTSRGDKILLFSYSVRMLDILEKFLIRKGYCFSRFDGSTPMNARQSLVDEFNRSPSKQVFLISTRAGNLGVNLVSANRVVIFDPSWNPAQDLQAQDRSFRYGQKRHVVVFRLLAAGSFEELVYSRQVYKQQLSNIAVSGKVEKRYFEGVQDCKKFQGELFGICNLFRDLSDKLFTSEIIEMHEEQGRNTGTDNVPVSLKIDVKSNANEANKVSNLLKSYGEATRAENEATLDDLGIVYAHRNEDVVNMKMGGNHGGISTHEDDTNKTFPTKAADRSHERKHVKLRGGEPSLAHQKRREFSRIARYMNMGELEFSKWLLSASYLERMEMLQNYKKKKKKKKKKKKQKVEVD

Target ORF information:

RefSeq Version	XM_020236672.1
Organism	Ananas comosus(pineapple)
Definition	Ananas comosus switch 2 (LOC109712878), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020236672.1

ORF Insert Sequence:

ATGTCCCTAA ACCGCCTCAA AGAAACCCTC CGATTCTCCT CCACCACCAC CACCACCTCC 
TCCTCCTCCG CCGCATCTTC TTTCTCCCTC TCCGAACCCC TTCCTCCTCT GAGGAAACCC 
CCCAAATCCT CGCTCTCTCA GCAGCTCCAA CGCCTCCACC TCCCCTCCTC CTCCTCCTCC 
TCCTCTTCCT CCTCCGATCC ACCTCCGCTT CCTCCGCCTC CGCCTCCGCC TCCGCCTCCG 
CCCCCACGAC GACGAGAGGA AGAAGAAGGC GACAAAGACG AGGACGAGGA CGAGGACGAC 
GATGGTGAAA GGGACGGTGC ACTATCTCGC CCTAATCGGG AGCCCGTTCT TTCTCAGCCT 
TTCTCTTTCG AGGTGCGGGG GCCTTACGAG CCCTTGGTCC TCTCGTCCCC CCGGGAGGAT 
CCGATCGTGC AGGTTCCTGC ATCAATCAAT TGTAGGCTTC TTGAACATCA AAGAGATGGC 
GTGAGGTTCT TGTATAATTT GTACAAGAAC AACCATGGTG GCGTACTTGG GGATGACATG 
ATTCATGACA AAATTTTATC TGAGATCTCA TGGGAAATTG TGATTGTTGA TGAAGCTCAT 
CGTTTAAAGA ATGAAAAATC TAAACTCTAT ACAGCATGTT TAGGAATAAG AACAAGCAAA 
AGGTATGGAC TCACAGGGAC TATAATGCAG AACAAGATAT TAGAATTATT TAATCTGTTT 
GACTGGGTTG CACCTGGGTC TTTGGGAGAT AGAGAGCATT TTCGAGCTTT TTATGATGAG 
CCATTAAAGC AAGGTCAGAG GTTCAGTGCT CCTGAAAGAT TTATTCAAAT TGCTGATGAG 
CGCAAAAAGC ACCTTGTTGC AGTTCTAAGA AAATATTTAT TAAGAAGGAC AAAGGAGGAG 
ACCATAGGGC ATCTGATGCT TGGAAAAGAA GATAACGTTG TTTTCTGCAA AATGAGTGAC 
CTACAGAAGC GTGTTTACAG GAGATTATTG CAACAACCTG ATATACAGTG CCTTGTAAAT 
AAAGATCTTC CTTGTAGCTG TGGAAGTCCT CTAACTCAGG TGGAGTGTTG TAAAAAAATT 
GAGCCTAAGG GCATTATTTG GTCTTATCTC CATAAGGACA ACCCCGATGG TTGTGCTTTG 
TGCCCTTTCT GCCTCGTTCT TCCTTGCCTA GTCAAGCTTC AGCAGATTAG CAATCACCTG 
GAGCTGATAA AGCCTAGCCC TAAGGATGAA AGTGAGAAAC AAAAGAAGGA TTCTGAACTA 
GCAGCTGCTG TCTTTGGTGA TGATATTGGC TTGGTTGGCG GTGGTGCTAA AAGTGAAAGT 
TTCATGGGCC TTAGTGATGC AGAACATTGT GGGAAAATGC GAGCATTGGA AAAGCTGTTG 
GCATCATGGA CTTCGCGTGG TGACAAGATA CTTCTATTCA GCTACTCTGT CAGGATGCTT 
GACATACTGG AGAAGTTCCT AATACGGAAA GGCTATTGCT TTTCCCGGTT CGACGGTTCA 
ACTCCCATGA ATGCACGTCA GTCACTTGTT GATGAGTTCA ACAGAAGTCC AAGTAAACAG 
GTTTTCCTAA TATCAACTCG GGCAGGTAAT CTAGGCGTGA ATCTTGTGAG TGCTAACCGT 
GTCGTAATAT TCGATCCGAG TTGGAATCCA GCACAAGATT TGCAGGCTCA AGACAGATCG 
TTTCGCTATG GACAGAAACG GCATGTTGTA GTATTTCGTT TACTTGCTGC TGGTTCGTTT 
GAAGAACTTG TTTATTCACG GCAAGTGTAT AAACAGCAGC TTTCCAATAT CGCTGTTTCG 
GGGAAAGTGG AAAAGCGTTA CTTTGAAGGT GTTCAGGACT GCAAGAAGTT TCAAGGAGAG 
TTATTTGGAA TTTGCAATCT GTTCCGAGAC CTGTCTGACA AGCTTTTTAC AAGTGAGATC 
ATTGAAATGC ATGAGGAGCA AGGGAGGAAT ACTGGAACAG ATAATGTGCC AGTAAGCCTG 
AAAATTGATG TTAAATCTAA TGCTAATGAA GCCAATAAGG TATCAAACTT ACTGAAGTCT 
TATGGGGAAG CAACTCGTGC TGAAAATGAG GCAACACTGG ATGATTTGGG CATAGTGTAT 
GCCCATCGCA ACGAAGACGT CGTTAACATG AAAATGGGAG GAAATCATGG TGGAATAAGT 
ACCCATGAAG ATGATACCAA CAAGACTTTT CCTACCAAGG CAGCAGATAG AAGCCATGAA 
AGGAAACATG TAAAGTTGAG AGGAGGAGAA CCTTCGTTAG CCCATCAAAA AAGAAGAGAG 
TTTAGTCGAA TTGCTCGATA CATGAATATG GGGGAGCTCG AGTTCAGTAA GTGGTTACTC 
TCTGCTTCTT ATTTAGAGAG GATGGAAATG CTACAGAACT ACAAGAAAAA GAAGAAGAAG 
AAGAAGAAGA AGAAGAAACA GAAGGTTGAA GTTGATTAG