CMT3 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	CMT3
Entrez Gene ID	843313
Full Name	chromomethylase 3
Synonyms	T6C23.3,T6C23_3,chromomethylase 3
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_105645.3	NP_177135.1	DNA (cytosine-5)-methyltransferase CMT3
NM_105645.4	NP_177135.1	chromomethylase 3

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	CMT1	NP_565245.1
Arabidopsis thaliana (thale cress)	CMT3	NP_177135.1
Arabidopsis thaliana (thale cress)	CMT2	NP_193637.2

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results suggest that dedifferentiation is associated with Tag1 activation and that CMT3 rather than DDM1 plays a central role in restraining Tag1 activation via inducing gene body DNA methylation.
Title: Activation of Tag1 transposable elements in Arabidopsis dedifferentiating cells and their regulation by CHROMOMETHYLASE 3-mediated CHG methylation.

The methylation level of the Arabidopsis genome, including transposons, was significantly lower in the siz1-2 mutant than in wild-type plants. CMT3 was sumoylated by the E3 ligase activity of AtSIZ1 through a direct interaction.
Title: Arabidopsis CMT3 activity is positively regulated by AtSIZ1-mediated sumoylation.

Cmt3gene expression throughout normal development in Arabidopsis
Title: [Developmental expression of Arabidopsis methyltransferase genes MET1, DRM2 and CMT3].

gamma irradiation induced DNA hypomethylation preferentially at non-CG sites of transcriptionally inactive repeats in a locus-specific manner, which depends on CMT3 activity
Title: Characterization of non-CG genomic hypomethylation associated with gamma-ray-induced suppression of CMT3 transcription in Arabidopsis thaliana.

These results suggest that CMT3 associates with H3K9me2-containing nucleosomes through dual binding of its bromo adjacent homology and chromo domains to H3K9me2 in order to target DNA methylation.
Title: Dual binding of chromomethylase domains to H3K9me2-containing nucleosomes directs DNA methylation in plants.

The following CMT3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CMT3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb27513	NM_105645.4 Latest version!	Arabidopsis thaliana chromomethylase 3 (CMT3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
OAb27513	NM_105645.3	Arabidopsis thaliana DNA (cytosine-5)-methyltransferase CMT3 mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CMT3 ( NM_105645.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_177135.1 Arabidopsis thaliana DNA (cytosine-5)-methyltransferase CMT3 mRNA, complete cds.

Price & Availability↑

CloneID	OAb27513
Clone ID Related Accession (Same CDS sequence)	NM_105645.3 , NM_105645.4
Accession Version	NM_105645.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-01-21
Organism	Arabidopsis thaliana(Thale cress)
Product	DNA (cytosine-5)-methyltransferase CMT3
Comment

ATGGCGCCGA AGCGAAAGAG ACCTGCGACA AAGGATGACA CTACCAAATC CATTCCCAAA 
CCGAAGAAGA GAGCTCCTAA GCGAGCTAAG ACGGTGAAAG AAGAGCCGGT GACAGTGGTC 
GAGGAAGGGG AAAAGCATGT TGCGAGGTTT CTAGACGAGC CAATTCCAGA ATCTGAAGCG 
AAGAGTACCT GGCCTGACAG ATACAAACCG ATTGAGGTAC AGCCACCTAA GGCTTCGTCA 
AGAAAGAAGA CGAAGGATGA CGAAAAAGTT GAGATCATTC GTGCTCGATG CCATTATAGA 
CGTGCGATTG TTGATGAGCG TCAGATATAT GAGCTGAATG ATGATGCTTA TGTACAGTCT 
GGTGAGGGAA AGGATCCCTT CATTTGTAAA ATCATTGAAA TGTTTGAAGG GGCTAATGGG 
AAACTGTATT TCACGGCTCG GTGGTTTTAT AGACCTTCTG ATACTGTAAT GAAAGAGTTC 
GAGATTCTGA TCAAGAAAAA GCGTGTGTTT TTCTCTGAGA TACAAGATAC AAATGAATTG 
GGATTACTTG AAAAGAAGCT GAACATTTTG ATGATTCCCT TGAATGAAAA TACTAAAGAG 
ACTATCCCTG CAACAGAAAA CTGTGACTTT TTCTGTGACA TGAACTATTT CTTGCCTTAC 
GATACATTTG AAGCTATACA ACAAGAAACC ATGATGGCTA TAAGTGAAAG TTCAACAATA 
TCCAGTGATA CTGATATAAG AGAAGGAGCT GCTGCCATAT CAGAGATTGG AGAATGTTCT 
CAAGAAACAG AAGGTCACAA AAAGGCAACT TTGCTTGACC TTTACTCCGG CTGTGGAGCT 
ATGTCGACAG GGTTGTGCAT GGGTGCACAA CTGTCTGGTT TGAACCTCGT CACTAAATGG 
GCTGTTGACA TGAATGCACA TGCATGTAAA AGCTTGCAGC ATAACCACCC AGAGACAAAC 
GTGAGAAACA TGACCGCAGA AGATTTCTTG TTTCTGCTTA AGGAGTGGGA GAAGCTATGC 
ATTCATTTCT CTTTGAGAAA TAGTCCAAAT TCAGAAGAAT ATGCCAACCT TCACGGTTTG 
AATAATGTTG AGGACAATGA AGATGTCAGC GAGGAGAGTG AAAATGAAGA TGATGGAGAA 
GTTTTTACTG TTGACAAGAT TGTTGGTATT TCCTTCGGAG TCCCTAAAAA GTTATTGAAA 
CGTGGACTTT ATTTGAAGGT AAGGTGGCTG AATTATGATG ATTCTCATGA TACATGGGAG 
CCTATTGAAG GACTCAGTAA TTGCCGGGGT AAAATTGAAG AGTTCGTTAA ACTTGGATAT 
AAATCTGGCA TCCTTCCGTT ACCAGGAGGT GTTGATGTTG TCTGCGGTGG GCCACCATGC 
CAAGGAATCA GTGGTCACAA CCGCTTCAGG AACTTATTGG ACCCTCTAGA AGATCAGAAA 
AACAAGCAGC TTTTGGTGTA TATGAACATT GTAGAATATT TGAAGCCTAA GTTCGTTTTG 
ATGGAAAACG TCGTTGACAT GCTGAAGATG GCTAAGGGCT ATCTTGCACG GTTTGCTGTT 
GGACGCCTTC TACAGATGAA TTACCAAGTG AGGAATGGAA TGATGGCAGC TGGAGCTTAT 
GGGCTTGCTC AGTTTCGTTT GAGGTTCTTT CTATGGGGTG CACTCCCTAG TGAGATAATT 
CCGCAGTTCC CACTTCCAAC ACATGATCTA GTTCATAGAG GAAATATTGT CAAGGAGTTT 
CAGGGAAACA TAGTAGCCTA TGATGAAGGA CATACTGTGA AGTTAGCAGA CAAGCTTTTG 
TTGAAGGATG TGATTTCTGA TCTTCCTGCA GTTGCCAACA GTGAAAAAAG AGACGAGATT 
ACATATGACA AAGATCCCAC AACGCCATTT CAAAAGTTCA TCAGATTGAG AAAGGATGAA 
GCGTCAGGTT CACAATCAAA GTCCAAGTCC AAAAAGCATG TCTTATATGA TCATCACCCT 
CTTAATCTTA ATATAAATGA CTATGAACGG GTTTGTCAGG TCCCCAAGAG AAAGGGAGCG 
AATTTTAGGG ACTTTCCTGG TGTTATTGTT GGACCTGGTA ATGTAGTCAA GTTGGAAGAG 
GGAAAGGAAA GGGTCAAACT TGAATCTGGA AAAACATTGG TTCCCGATTA TGCCTTAACA 
TATGTCGATG GGAAATCATG CAAACCTTTT GGTCGTCTTT GGTGGGACGA AATTGTCCCC 
ACTGTTGTCA CACGGGCAGA ACCCCACAAC CAGGTGATCA TTCATCCAGA GCAAAATCGG 
GTTTTATCCA TTCGAGAAAA TGCGAGACTC CAAGGCTTTC CTGATGACTA CAAACTCTTT 
GGCCCACCCA AACAGAAGTA CATTCAAGTA GGTAACGCTG TAGCTGTGCC AGTAGCGAAG 
GCCCTTGGAT ATGCTTTGGG AACAGCTTTC CAGGGACTCG CAGTTGGGAA AGATCCACTT 
CTTACTCTGC CTGAAGGTTT TGCATTCATG AAGCCAACTC TTCCTTCCGA GCTTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_177135.1

Translation

MAPKRKRPATKDDTTKSIPKPKKRAPKRAKTVKEEPVTVVEEGEKHVARFLDEPIPESEAKSTWPDRYKPIEVQPPKASSRKKTKDDEKVEIIRARCHYRRAIVDERQIYELNDDAYVQSGEGKDPFICKIIEMFEGANGKLYFTARWFYRPSDTVMKEFEILIKKKRVFFSEIQDTNELGLLEKKLNILMIPLNENTKETIPATENCDFFCDMNYFLPYDTFEAIQQETMMAISESSTISSDTDIREGAAAISEIGECSQETEGHKKATLLDLYSGCGAMSTGLCMGAQLSGLNLVTKWAVDMNAHACKSLQHNHPETNVRNMTAEDFLFLLKEWEKLCIHFSLRNSPNSEEYANLHGLNNVEDNEDVSEESENEDDGEVFTVDKIVGISFGVPKKLLKRGLYLKVRWLNYDDSHDTWEPIEGLSNCRGKIEEFVKLGYKSGILPLPGGVDVVCGGPPCQGISGHNRFRNLLDPLEDQKNKQLLVYMNIVEYLKPKFVLMENVVDMLKMAKGYLARFAVGRLLQMNYQVRNGMMAAGAYGLAQFRLRFFLWGALPSEIIPQFPLPTHDLVHRGNIVKEFQGNIVAYDEGHTVKLADKLLLKDVISDLPAVANSEKRDEITYDKDPTTPFQKFIRLRKDEASGSQSKSKSKKHVLYDHHPLNLNINDYERVCQVPKRKGANFRDFPGVIVGPGNVVKLEEGKERVKLESGKTLVPDYALTYVDGKSCKPFGRLWWDEIVPTVVTRAEPHNQVIIHPEQNRVLSIRENARLQGFPDDYKLFGPPKQKYIQVGNAVAVPVAKALGYALGTAFQGLAVGKDPLLTLPEGFAFMKPTLPSELA

Target ORF information:

RefSeq Version	NM_105645.3
Organism	Arabidopsis thaliana(Thale cress)
Definition	Arabidopsis thaliana DNA (cytosine-5)-methyltransferase CMT3 mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_105645.3

ORF Insert Sequence:

ATGGCGCCGA AGCGAAAGAG ACCTGCGACA AAGGATGACA CTACCAAATC CATTCCCAAA 
CCGAAGAAGA GAGCTCCTAA GCGAGCTAAG ACGGTGAAAG AAGAGCCGGT GACAGTGGTC 
GAGGAAGGGG AAAAGCATGT TGCGAGGTTT CTAGACGAGC CAATTCCAGA ATCTGAAGCG 
AAGAGTACCT GGCCTGACAG ATACAAACCG ATTGAGGTAC AGCCACCTAA GGCTTCGTCA 
AGAAAGAAGA CGAAGGATGA CGAAAAAGTT GAGATCATTC GTGCTCGATG CCATTATAGA 
CGTGCGATTG TTGATGAGCG TCAGATATAT GAGCTGAATG ATGATGCTTA TGTACAGTCT 
GGTGAGGGAA AGGATCCCTT CATTTGTAAA ATCATTGAAA TGTTTGAAGG GGCTAATGGG 
AAACTGTATT TCACGGCTCG GTGGTTTTAT AGACCTTCTG ATACTGTAAT GAAAGAGTTC 
GAGATTCTGA TCAAGAAAAA GCGTGTGTTT TTCTCTGAGA TACAAGATAC AAATGAATTG 
GGATTACTTG AAAAGAAGCT GAACATTTTG ATGATTCCCT TGAATGAAAA TACTAAAGAG 
ACTATCCCTG CAACAGAAAA CTGTGACTTT TTCTGTGACA TGAACTATTT CTTGCCTTAC 
GATACATTTG AAGCTATACA ACAAGAAACC ATGATGGCTA TAAGTGAAAG TTCAACAATA 
TCCAGTGATA CTGATATAAG AGAAGGAGCT GCTGCCATAT CAGAGATTGG AGAATGTTCT 
CAAGAAACAG AAGGTCACAA AAAGGCAACT TTGCTTGACC TTTACTCCGG CTGTGGAGCT 
ATGTCGACAG GGTTGTGCAT GGGTGCACAA CTGTCTGGTT TGAACCTCGT CACTAAATGG 
GCTGTTGACA TGAATGCACA TGCATGTAAA AGCTTGCAGC ATAACCACCC AGAGACAAAC 
GTGAGAAACA TGACCGCAGA AGATTTCTTG TTTCTGCTTA AGGAGTGGGA GAAGCTATGC 
ATTCATTTCT CTTTGAGAAA TAGTCCAAAT TCAGAAGAAT ATGCCAACCT TCACGGTTTG 
AATAATGTTG AGGACAATGA AGATGTCAGC GAGGAGAGTG AAAATGAAGA TGATGGAGAA 
GTTTTTACTG TTGACAAGAT TGTTGGTATT TCCTTCGGAG TCCCTAAAAA GTTATTGAAA 
CGTGGACTTT ATTTGAAGGT AAGGTGGCTG AATTATGATG ATTCTCATGA TACATGGGAG 
CCTATTGAAG GACTCAGTAA TTGCCGGGGT AAAATTGAAG AGTTCGTTAA ACTTGGATAT 
AAATCTGGCA TCCTTCCGTT ACCAGGAGGT GTTGATGTTG TCTGCGGTGG GCCACCATGC 
CAAGGAATCA GTGGTCACAA CCGCTTCAGG AACTTATTGG ACCCTCTAGA AGATCAGAAA 
AACAAGCAGC TTTTGGTGTA TATGAACATT GTAGAATATT TGAAGCCTAA GTTCGTTTTG 
ATGGAAAACG TCGTTGACAT GCTGAAGATG GCTAAGGGCT ATCTTGCACG GTTTGCTGTT 
GGACGCCTTC TACAGATGAA TTACCAAGTG AGGAATGGAA TGATGGCAGC TGGAGCTTAT 
GGGCTTGCTC AGTTTCGTTT GAGGTTCTTT CTATGGGGTG CACTCCCTAG TGAGATAATT 
CCGCAGTTCC CACTTCCAAC ACATGATCTA GTTCATAGAG GAAATATTGT CAAGGAGTTT 
CAGGGAAACA TAGTAGCCTA TGATGAAGGA CATACTGTGA AGTTAGCAGA CAAGCTTTTG 
TTGAAGGATG TGATTTCTGA TCTTCCTGCA GTTGCCAACA GTGAAAAAAG AGACGAGATT 
ACATATGACA AAGATCCCAC AACGCCATTT CAAAAGTTCA TCAGATTGAG AAAGGATGAA 
GCGTCAGGTT CACAATCAAA GTCCAAGTCC AAAAAGCATG TCTTATATGA TCATCACCCT 
CTTAATCTTA ATATAAATGA CTATGAACGG GTTTGTCAGG TCCCCAAGAG AAAGGGAGCG 
AATTTTAGGG ACTTTCCTGG TGTTATTGTT GGACCTGGTA ATGTAGTCAA GTTGGAAGAG 
GGAAAGGAAA GGGTCAAACT TGAATCTGGA AAAACATTGG TTCCCGATTA TGCCTTAACA 
TATGTCGATG GGAAATCATG CAAACCTTTT GGTCGTCTTT GGTGGGACGA AATTGTCCCC 
ACTGTTGTCA CACGGGCAGA ACCCCACAAC CAGGTGATCA TTCATCCAGA GCAAAATCGG 
GTTTTATCCA TTCGAGAAAA TGCGAGACTC CAAGGCTTTC CTGATGACTA CAAACTCTTT 
GGCCCACCCA AACAGAAGTA CATTCAAGTA GGTAACGCTG TAGCTGTGCC AGTAGCGAAG 
GCCCTTGGAT ATGCTTTGGG AACAGCTTTC CAGGGACTCG CAGTTGGGAA AGATCCACTT 
CTTACTCTGC CTGAAGGTTT TGCATTCATG AAGCCAACTC TTCCTTCCGA GCTTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CMT3 ( NM_105645.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_177135.1 Arabidopsis thaliana chromomethylase 3 (CMT3), mRNA.

Price & Availability↑

CloneID	OAb27513
Clone ID Related Accession (Same CDS sequence)	NM_105645.3 , NM_105645.4
Accession Version	NM_105645.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	chromomethylase 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_105645.3.

ATGGCGCCGA AGCGAAAGAG ACCTGCGACA AAGGATGACA CTACCAAATC CATTCCCAAA 
CCGAAGAAGA GAGCTCCTAA GCGAGCTAAG ACGGTGAAAG AAGAGCCGGT GACAGTGGTC 
GAGGAAGGGG AAAAGCATGT TGCGAGGTTT CTAGACGAGC CAATTCCAGA ATCTGAAGCG 
AAGAGTACCT GGCCTGACAG ATACAAACCG ATTGAGGTAC AGCCACCTAA GGCTTCGTCA 
AGAAAGAAGA CGAAGGATGA CGAAAAAGTT GAGATCATTC GTGCTCGATG CCATTATAGA 
CGTGCGATTG TTGATGAGCG TCAGATATAT GAGCTGAATG ATGATGCTTA TGTACAGTCT 
GGTGAGGGAA AGGATCCCTT CATTTGTAAA ATCATTGAAA TGTTTGAAGG GGCTAATGGG 
AAACTGTATT TCACGGCTCG GTGGTTTTAT AGACCTTCTG ATACTGTAAT GAAAGAGTTC 
GAGATTCTGA TCAAGAAAAA GCGTGTGTTT TTCTCTGAGA TACAAGATAC AAATGAATTG 
GGATTACTTG AAAAGAAGCT GAACATTTTG ATGATTCCCT TGAATGAAAA TACTAAAGAG 
ACTATCCCTG CAACAGAAAA CTGTGACTTT TTCTGTGACA TGAACTATTT CTTGCCTTAC 
GATACATTTG AAGCTATACA ACAAGAAACC ATGATGGCTA TAAGTGAAAG TTCAACAATA 
TCCAGTGATA CTGATATAAG AGAAGGAGCT GCTGCCATAT CAGAGATTGG AGAATGTTCT 
CAAGAAACAG AAGGTCACAA AAAGGCAACT TTGCTTGACC TTTACTCCGG CTGTGGAGCT 
ATGTCGACAG GGTTGTGCAT GGGTGCACAA CTGTCTGGTT TGAACCTCGT CACTAAATGG 
GCTGTTGACA TGAATGCACA TGCATGTAAA AGCTTGCAGC ATAACCACCC AGAGACAAAC 
GTGAGAAACA TGACCGCAGA AGATTTCTTG TTTCTGCTTA AGGAGTGGGA GAAGCTATGC 
ATTCATTTCT CTTTGAGAAA TAGTCCAAAT TCAGAAGAAT ATGCCAACCT TCACGGTTTG 
AATAATGTTG AGGACAATGA AGATGTCAGC GAGGAGAGTG AAAATGAAGA TGATGGAGAA 
GTTTTTACTG TTGACAAGAT TGTTGGTATT TCCTTCGGAG TCCCTAAAAA GTTATTGAAA 
CGTGGACTTT ATTTGAAGGT AAGGTGGCTG AATTATGATG ATTCTCATGA TACATGGGAG 
CCTATTGAAG GACTCAGTAA TTGCCGGGGT AAAATTGAAG AGTTCGTTAA ACTTGGATAT 
AAATCTGGCA TCCTTCCGTT ACCAGGAGGT GTTGATGTTG TCTGCGGTGG GCCACCATGC 
CAAGGAATCA GTGGTCACAA CCGCTTCAGG AACTTATTGG ACCCTCTAGA AGATCAGAAA 
AACAAGCAGC TTTTGGTGTA TATGAACATT GTAGAATATT TGAAGCCTAA GTTCGTTTTG 
ATGGAAAACG TCGTTGACAT GCTGAAGATG GCTAAGGGCT ATCTTGCACG GTTTGCTGTT 
GGACGCCTTC TACAGATGAA TTACCAAGTG AGGAATGGAA TGATGGCAGC TGGAGCTTAT 
GGGCTTGCTC AGTTTCGTTT GAGGTTCTTT CTATGGGGTG CACTCCCTAG TGAGATAATT 
CCGCAGTTCC CACTTCCAAC ACATGATCTA GTTCATAGAG GAAATATTGT CAAGGAGTTT 
CAGGGAAACA TAGTAGCCTA TGATGAAGGA CATACTGTGA AGTTAGCAGA CAAGCTTTTG 
TTGAAGGATG TGATTTCTGA TCTTCCTGCA GTTGCCAACA GTGAAAAAAG AGACGAGATT 
ACATATGACA AAGATCCCAC AACGCCATTT CAAAAGTTCA TCAGATTGAG AAAGGATGAA 
GCGTCAGGTT CACAATCAAA GTCCAAGTCC AAAAAGCATG TCTTATATGA TCATCACCCT 
CTTAATCTTA ATATAAATGA CTATGAACGG GTTTGTCAGG TCCCCAAGAG AAAGGGAGCG 
AATTTTAGGG ACTTTCCTGG TGTTATTGTT GGACCTGGTA ATGTAGTCAA GTTGGAAGAG 
GGAAAGGAAA GGGTCAAACT TGAATCTGGA AAAACATTGG TTCCCGATTA TGCCTTAACA 
TATGTCGATG GGAAATCATG CAAACCTTTT GGTCGTCTTT GGTGGGACGA AATTGTCCCC 
ACTGTTGTCA CACGGGCAGA ACCCCACAAC CAGGTGATCA TTCATCCAGA GCAAAATCGG 
GTTTTATCCA TTCGAGAAAA TGCGAGACTC CAAGGCTTTC CTGATGACTA CAAACTCTTT 
GGCCCACCCA AACAGAAGTA CATTCAAGTA GGTAACGCTG TAGCTGTGCC AGTAGCGAAG 
GCCCTTGGAT ATGCTTTGGG AACAGCTTTC CAGGGACTCG CAGTTGGGAA AGATCCACTT 
CTTACTCTGC CTGAAGGTTT TGCATTCATG AAGCCAACTC TTCCTTCCGA GCTTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_177135.1
CDS	169..2688
Translation	MAPKRKRPATKDDTTKSIPKPKKRAPKRAKTVKEEPVTVVEEGEKHVARFLDEPIPESEAKSTWPDRYKPIEVQPPKASSRKKTKDDEKVEIIRARCHYRRAIVDERQIYELNDDAYVQSGEGKDPFICKIIEMFEGANGKLYFTARWFYRPSDTVMKEFEILIKKKRVFFSEIQDTNELGLLEKKLNILMIPLNENTKETIPATENCDFFCDMNYFLPYDTFEAIQQETMMAISESSTISSDTDIREGAAAISEIGECSQETEGHKKATLLDLYSGCGAMSTGLCMGAQLSGLNLVTKWAVDMNAHACKSLQHNHPETNVRNMTAEDFLFLLKEWEKLCIHFSLRNSPNSEEYANLHGLNNVEDNEDVSEESENEDDGEVFTVDKIVGISFGVPKKLLKRGLYLKVRWLNYDDSHDTWEPIEGLSNCRGKIEEFVKLGYKSGILPLPGGVDVVCGGPPCQGISGHNRFRNLLDPLEDQKNKQLLVYMNIVEYLKPKFVLMENVVDMLKMAKGYLARFAVGRLLQMNYQVRNGMMAAGAYGLAQFRLRFFLWGALPSEIIPQFPLPTHDLVHRGNIVKEFQGNIVAYDEGHTVKLADKLLLKDVISDLPAVANSEKRDEITYDKDPTTPFQKFIRLRKDEASGSQSKSKSKKHVLYDHHPLNLNINDYERVCQVPKRKGANFRDFPGVIVGPGNVVKLEEGKERVKLESGKTLVPDYALTYVDGKSCKPFGRLWWDEIVPTVVTRAEPHNQVIIHPEQNRVLSIRENARLQGFPDDYKLFGPPKQKYIQVGNAVAVPVAKALGYALGTAFQGLAVGKDPLLTLPEGFAFMKPTLPSELA

Target ORF information:

RefSeq Version	NM_105645.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana chromomethylase 3 (CMT3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_105645.4

ORF Insert Sequence:

ATGGCGCCGA AGCGAAAGAG ACCTGCGACA AAGGATGACA CTACCAAATC CATTCCCAAA 
CCGAAGAAGA GAGCTCCTAA GCGAGCTAAG ACGGTGAAAG AAGAGCCGGT GACAGTGGTC 
GAGGAAGGGG AAAAGCATGT TGCGAGGTTT CTAGACGAGC CAATTCCAGA ATCTGAAGCG 
AAGAGTACCT GGCCTGACAG ATACAAACCG ATTGAGGTAC AGCCACCTAA GGCTTCGTCA 
AGAAAGAAGA CGAAGGATGA CGAAAAAGTT GAGATCATTC GTGCTCGATG CCATTATAGA 
CGTGCGATTG TTGATGAGCG TCAGATATAT GAGCTGAATG ATGATGCTTA TGTACAGTCT 
GGTGAGGGAA AGGATCCCTT CATTTGTAAA ATCATTGAAA TGTTTGAAGG GGCTAATGGG 
AAACTGTATT TCACGGCTCG GTGGTTTTAT AGACCTTCTG ATACTGTAAT GAAAGAGTTC 
GAGATTCTGA TCAAGAAAAA GCGTGTGTTT TTCTCTGAGA TACAAGATAC AAATGAATTG 
GGATTACTTG AAAAGAAGCT GAACATTTTG ATGATTCCCT TGAATGAAAA TACTAAAGAG 
ACTATCCCTG CAACAGAAAA CTGTGACTTT TTCTGTGACA TGAACTATTT CTTGCCTTAC 
GATACATTTG AAGCTATACA ACAAGAAACC ATGATGGCTA TAAGTGAAAG TTCAACAATA 
TCCAGTGATA CTGATATAAG AGAAGGAGCT GCTGCCATAT CAGAGATTGG AGAATGTTCT 
CAAGAAACAG AAGGTCACAA AAAGGCAACT TTGCTTGACC TTTACTCCGG CTGTGGAGCT 
ATGTCGACAG GGTTGTGCAT GGGTGCACAA CTGTCTGGTT TGAACCTCGT CACTAAATGG 
GCTGTTGACA TGAATGCACA TGCATGTAAA AGCTTGCAGC ATAACCACCC AGAGACAAAC 
GTGAGAAACA TGACCGCAGA AGATTTCTTG TTTCTGCTTA AGGAGTGGGA GAAGCTATGC 
ATTCATTTCT CTTTGAGAAA TAGTCCAAAT TCAGAAGAAT ATGCCAACCT TCACGGTTTG 
AATAATGTTG AGGACAATGA AGATGTCAGC GAGGAGAGTG AAAATGAAGA TGATGGAGAA 
GTTTTTACTG TTGACAAGAT TGTTGGTATT TCCTTCGGAG TCCCTAAAAA GTTATTGAAA 
CGTGGACTTT ATTTGAAGGT AAGGTGGCTG AATTATGATG ATTCTCATGA TACATGGGAG 
CCTATTGAAG GACTCAGTAA TTGCCGGGGT AAAATTGAAG AGTTCGTTAA ACTTGGATAT 
AAATCTGGCA TCCTTCCGTT ACCAGGAGGT GTTGATGTTG TCTGCGGTGG GCCACCATGC 
CAAGGAATCA GTGGTCACAA CCGCTTCAGG AACTTATTGG ACCCTCTAGA AGATCAGAAA 
AACAAGCAGC TTTTGGTGTA TATGAACATT GTAGAATATT TGAAGCCTAA GTTCGTTTTG 
ATGGAAAACG TCGTTGACAT GCTGAAGATG GCTAAGGGCT ATCTTGCACG GTTTGCTGTT 
GGACGCCTTC TACAGATGAA TTACCAAGTG AGGAATGGAA TGATGGCAGC TGGAGCTTAT 
GGGCTTGCTC AGTTTCGTTT GAGGTTCTTT CTATGGGGTG CACTCCCTAG TGAGATAATT 
CCGCAGTTCC CACTTCCAAC ACATGATCTA GTTCATAGAG GAAATATTGT CAAGGAGTTT 
CAGGGAAACA TAGTAGCCTA TGATGAAGGA CATACTGTGA AGTTAGCAGA CAAGCTTTTG 
TTGAAGGATG TGATTTCTGA TCTTCCTGCA GTTGCCAACA GTGAAAAAAG AGACGAGATT 
ACATATGACA AAGATCCCAC AACGCCATTT CAAAAGTTCA TCAGATTGAG AAAGGATGAA 
GCGTCAGGTT CACAATCAAA GTCCAAGTCC AAAAAGCATG TCTTATATGA TCATCACCCT 
CTTAATCTTA ATATAAATGA CTATGAACGG GTTTGTCAGG TCCCCAAGAG AAAGGGAGCG 
AATTTTAGGG ACTTTCCTGG TGTTATTGTT GGACCTGGTA ATGTAGTCAA GTTGGAAGAG 
GGAAAGGAAA GGGTCAAACT TGAATCTGGA AAAACATTGG TTCCCGATTA TGCCTTAACA 
TATGTCGATG GGAAATCATG CAAACCTTTT GGTCGTCTTT GGTGGGACGA AATTGTCCCC 
ACTGTTGTCA CACGGGCAGA ACCCCACAAC CAGGTGATCA TTCATCCAGA GCAAAATCGG 
GTTTTATCCA TTCGAGAAAA TGCGAGACTC CAAGGCTTTC CTGATGACTA CAAACTCTTT 
GGCCCACCCA AACAGAAGTA CATTCAAGTA GGTAACGCTG TAGCTGTGCC AGTAGCGAAG 
GCCCTTGGAT ATGCTTTGGG AACAGCTTTC CAGGGACTCG CAGTTGGGAA AGATCCACTT 
CTTACTCTGC CTGAAGGTTT TGCATTCATG AAGCCAACTC TTCCTTCCGA GCTTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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CMT3 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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