RCF3 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	RCF3
Entrez Gene ID	835386
Full Name	RNA-binding KH domain-containing protein
Synonyms	MNB8.12,MNB8_12,SHI1,SHINY 1,regulator of CBF gene expression 3
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001345032.1	NP_001330551.1	RNA-binding KH domain-containing protein
NM_001203597.1	NP_001190526.1	RNA-binding KH domain-containing protein
NM_124685.4	NP_200118.3	RNA-binding KH domain-containing protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

KH domain protein RCF3 is a tissue-biased regulator of the plant miRNA biogenesis cofactor HYL1.
Title: KH domain protein RCF3 is a tissue-biased regulator of the plant miRNA biogenesis cofactor HYL1.

HIGH OSMOTIC STRESS GENE EXPRESSION 5 (HOS5)affects the biogenesis of a subset of miRNA.
Title: The RNA-binding protein HOS5 and serine/arginine-rich proteins RS40 and RS41 participate in miRNA biogenesis in Arabidopsis.

a KH domain-containing putative RNA-binding protein RCF3 is an important upstream regulator for heat stress-responsive gene expression and thermotolerance in Arabidopsis [RCF3]
Title: A KH domain-containing putative RNA-binding protein is critical for heat stress-responsive gene regulation and thermotolerance in Arabidopsis.

In fry2 mutants, HOS5 failed to be localized in nuclear speckles but was found mainly in the nucleoplasm. hos5 mutants were impaired in mRNA export and accumulated a significant amount of mRNA in the nuclei, particularly under salt stress conditions. [HOS5]
Title: A KH-domain RNA-binding protein interacts with FIERY2/CTD phosphatase-like 1 and splicing factors and is important for pre-mRNA splicing in Arabidopsis.

K-homology domain-containing protein named SHINY1 (SHI1) interacts with CPL1 to modulate gene expression. [SHI1] [SHINY1]
Title: The arabidopsis RNA binding protein with K homology motifs, SHINY1, interacts with the C-terminal domain phosphatase-like 1 (CPL1) to repress stress-inducible gene expression.

The following RCF3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RCF3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb21323	NM_001203597.1 Latest version!	Arabidopsis thaliana RNA-binding KH domain-containing protein (RCF3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OAb30687	NM_124685.4 Latest version!	Arabidopsis thaliana RNA-binding KH domain-containing protein (RCF3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OAb41873	NM_001345032.1 Latest version!	Arabidopsis thaliana RNA-binding KH domain-containing protein (RCF3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RCF3 ( NM_001203597.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001190526.1 Arabidopsis thaliana RNA-binding KH domain-containing protein (RCF3), mRNA.

Price & Availability↑

CloneID	OAb21323
Clone ID Related Accession (Same CDS sequence)	NM_001203597.1
Accession Version	NM_001203597.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	RNA-binding KH domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGGAGAGAT CTAGATCCAA GAGAAACTAC CACTATGACC AAGACTATGA CGGAGATTCC 
ATGCCTAGGT CTAAACCTCG CTACAACAAT AATTACCACT TTGGTGGTGG CGGCGGAGGA 
AATAACAGAT ACCGTGGCGG CGGCGGTGGT GGAGGTGGAA ATGGCCGGCC ATCGAAGTCT 
CATCCTGAAA CGATGGCAAC AACGACTTAT CGAATCCTCT GTCACGACGC TAAAGCAGGA 
GGTGTTATTG GTAAATCCGG GACAATTATC AAATCTATAA GGCAACACAC CGGCGCGTGG 
ATTAACGTGC ACGAGCTAGT TCCTGGAGAT GCAGAGCGTA TCATTGAGAT TTCTGATAAT 
CGTAGGCGTG ACCCTGATGG GAGAATGCCG TCTTTCTCTC CTGCTCAAGA GGCTTTGTTT 
AGTGTTCATG ACAGGATTTT GGAGAGTGAA GCTCAGTTTG GTTATGGTGG ACCTCCGCCG 
GAAGAGGAGG AGGATTACGG TGGAGTTAGA CCTGGTGGAG GGAGAGTTGT GACTAGATTA 
GTGGTTTCTA GGATGCATGT TGGTTGTTTG TTGGTTTTTT TTTTTTTTTT TTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001190526.1
CDS	330..926
Translation	MERSRSKRNYHYDQDYDGDSMPRSKPRYNNNYHFGGGGGGNNRYRGGGGGGGGNGRPSKSHPETMATTTYRILCHDAKAGGVIGKSGTIIKSIRQHTGAWINVHELVPGDAERIIEISDNRRRDPDGRMPSFSPAQEALFSVHDRILESEAQFGYGGPPPEEEEDYGGVRPGGGRVVTRLVVSRMHVGCLLVFFFFFL

Target ORF information:

RefSeq Version	NM_001203597.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana RNA-binding KH domain-containing protein (RCF3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001203597.1

ORF Insert Sequence:

ATGGAGAGAT CTAGATCCAA GAGAAACTAC CACTATGACC AAGACTATGA CGGAGATTCC 
ATGCCTAGGT CTAAACCTCG CTACAACAAT AATTACCACT TTGGTGGTGG CGGCGGAGGA 
AATAACAGAT ACCGTGGCGG CGGCGGTGGT GGAGGTGGAA ATGGCCGGCC ATCGAAGTCT 
CATCCTGAAA CGATGGCAAC AACGACTTAT CGAATCCTCT GTCACGACGC TAAAGCAGGA 
GGTGTTATTG GTAAATCCGG GACAATTATC AAATCTATAA GGCAACACAC CGGCGCGTGG 
ATTAACGTGC ACGAGCTAGT TCCTGGAGAT GCAGAGCGTA TCATTGAGAT TTCTGATAAT 
CGTAGGCGTG ACCCTGATGG GAGAATGCCG TCTTTCTCTC CTGCTCAAGA GGCTTTGTTT 
AGTGTTCATG ACAGGATTTT GGAGAGTGAA GCTCAGTTTG GTTATGGTGG ACCTCCGCCG 
GAAGAGGAGG AGGATTACGG TGGAGTTAGA CCTGGTGGAG GGAGAGTTGT GACTAGATTA 
GTGGTTTCTA GGATGCATGT TGGTTGTTTG TTGGTTTTTT TTTTTTTTTT TTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RCF3 ( NM_124685.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_200118.3 Arabidopsis thaliana RNA-binding KH domain-containing protein (RCF3), mRNA.

Price & Availability↑

CloneID	OAb30687
Clone ID Related Accession (Same CDS sequence)	NM_124685.4
Accession Version	NM_124685.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	RNA-binding KH domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_124685.3.

ATGGAGAGAT CTAGATCCAA GAGAAACTAC CACTATGACC AAGACTATGA CGGAGATTCC 
ATGCCTAGGT CTAAACCTCG CTACAACAAT AATTACCACT TTGGTGGTGG CGGCGGAGGA 
AATAACAGAT ACCGTGGCGG CGGCGGTGGT GGAGGTGGAA ATGGCCGGCC ATCGAAGTCT 
CATCCTGAAA CGATGGCAAC AACGACTTAT CGAATCCTCT GTCACGACGC TAAAGCAGGA 
GGTGTTATTG GTAAATCCGG GACAATTATC AAATCTATAA GGCAACACAC CGGCGCGTGG 
ATTAACGTGC ACGAGCTAGT TCCTGGAGAT GCAGAGCGTA TCATTGAGAT TTCTGATAAT 
CGTAGGCGTG ACCCTGATGG GAGAATGCCG TCTTTCTCTC CTGCTCAAGA GGCTTTGTTT 
AGTGTTCATG ACAGGATTTT GGAGAGTGAA GCTCAGTTTG GTTATGGTGG ACCTCCGCCG 
GAAGAGGAGG AGGATTACGG TGGAGTTAGA CCTGGTGGAG GGAGAGTTGT GACTAGATTA 
GTGGTTTCTA GGATGCATGT TGGTTGTTTG TTGGGTAAAG GTGGGAAGAT TATTGAGCAG 
ATGAGGATTG AAACTAAGAC TCATATTCGG ATTCTTCCAA GAGAAAGTAA CTTACCTCGT 
TGTGTTTCAC TCTCTGAAGA GATTGTTCAG ATTGTAGGTG AACTAAATGC AGTGAAAAAC 
GCTTTAGCCA TTGTATCATC TCGTCTAAGG GAGAGTCAGC ATCGTGATCG GAGTAATTTC 
CAAGGGCGTT CACATTCACC AGAACGGTCG TTTGCAGCTG CGGGGGATGA TTACATGCCA 
CAGCTAAGAA GACAATCATC CGATAGGTTT CCTCGTGGTA ATTTTAGAAA TAACAACTTC 
AGTTCTCGCC AGTCCAACTA TGCAGAAGAA GCTCCAGCAG TTCCAGTTGG TGAGAATGTC 
TACTCCGAAG AGCTTGTGTT CCAGATTCTC TGCCCAGCTG ACAAGATTGT TCGGGTTGTT 
GGGGAATCGC AAGGAATAAT TGATTTGCTT CAGAACGAGA TCGGCGTGGA CGTTAGAGTA 
TCTGACCCAG TTGCTGGATC TGATGAACAG ATAATCACCA TTTCTTCGGA AGAGGCACCT 
GATGATCCAT TTTTCCCTGC TCAAGAAGCC TTGTTGCACA TTCAAACTCA GATCATAGAT 
CTTATTCCGG ATAAAGATAA TTTAATAACA ACAAGATTAC TTGTCCCATC TAGAGACTCT 
ATATGTTTGG AAGGGAAAGC AGGATCAGTA TCTGAGATAT CACGATTAAC TGGAACAAGC 
GTACAAATTC TAGCTAGGGA AGAAATTCCC CGATGTGCTT CTATCAATGA TGTTGTTATA 
CAGATTACTG GCGAGATCAG AGCTGCTCGG GAAGCACTTG TTGAATTAAC ATTACTATTG 
AGGAGTCATA TGTTCAAGGA GCTCTCTCAA AAGGAGACAC CACCAGCTTC CACATCCACA 
ACTGGCCCTC TCGAAGGTGT TGCAGGAGTA ATGGAGGTAG CTTCATCAAA TAACACAATT 
CAATCTCGTG AAGGTCCTAC TAGTTCGAAT CTGAACTTGC AGCAAGTTAG CACAATCTTG 
CCACAGTTTA AGGAAGGTTT CGGATCAGTT GCAAAGGCGG GTGAAAGTGA ACACCGGGAA 
GAGGTTCCTG TCACGACAAG CAGAATGGCG GTACCACTTG TTACTAGAAG CACTCTTGAA 
GTTGTCTTAC CAGAGGCGGT TGTTCCTAAG CTCGTTACGA AATCAAGAAA CAAGCTTGCA 
CAAATCAGTG AGTGGTCTGG AGCTAGTGTA ACCATAGTTG AAGACAGACC AGAAGAAACA 
CAGAATATCA TTCGAATTTC GGGTACACCC GAGCAAGCAG AGAGAGCTCA GAGTTTGCTT 
CAAGGCTTCA TTTTGAGCAT ACAAGAGGAT GGACCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_200118.3
CDS	460..2418
Translation	MERSRSKRNYHYDQDYDGDSMPRSKPRYNNNYHFGGGGGGNNRYRGGGGGGGGNGRPSKSHPETMATTTYRILCHDAKAGGVIGKSGTIIKSIRQHTGAWINVHELVPGDAERIIEISDNRRRDPDGRMPSFSPAQEALFSVHDRILESEAQFGYGGPPPEEEEDYGGVRPGGGRVVTRLVVSRMHVGCLLGKGGKIIEQMRIETKTHIRILPRESNLPRCVSLSEEIVQIVGELNAVKNALAIVSSRLRESQHRDRSNFQGRSHSPERSFAAAGDDYMPQLRRQSSDRFPRGNFRNNNFSSRQSNYAEEAPAVPVGENVYSEELVFQILCPADKIVRVVGESQGIIDLLQNEIGVDVRVSDPVAGSDEQIITISSEEAPDDPFFPAQEALLHIQTQIIDLIPDKDNLITTRLLVPSRDSICLEGKAGSVSEISRLTGTSVQILAREEIPRCASINDVVIQITGEIRAAREALVELTLLLRSHMFKELSQKETPPASTSTTGPLEGVAGVMEVASSNNTIQSREGPTSSNLNLQQVSTILPQFKEGFGSVAKAGESEHREEVPVTTSRMAVPLVTRSTLEVVLPEAVVPKLVTKSRNKLAQISEWSGASVTIVEDRPEETQNIIRISGTPEQAERAQSLLQGFILSIQEDGP

Target ORF information:

RefSeq Version	NM_124685.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana RNA-binding KH domain-containing protein (RCF3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_124685.4

ORF Insert Sequence:

ATGGAGAGAT CTAGATCCAA GAGAAACTAC CACTATGACC AAGACTATGA CGGAGATTCC 
ATGCCTAGGT CTAAACCTCG CTACAACAAT AATTACCACT TTGGTGGTGG CGGCGGAGGA 
AATAACAGAT ACCGTGGCGG CGGCGGTGGT GGAGGTGGAA ATGGCCGGCC ATCGAAGTCT 
CATCCTGAAA CGATGGCAAC AACGACTTAT CGAATCCTCT GTCACGACGC TAAAGCAGGA 
GGTGTTATTG GTAAATCCGG GACAATTATC AAATCTATAA GGCAACACAC CGGCGCGTGG 
ATTAACGTGC ACGAGCTAGT TCCTGGAGAT GCAGAGCGTA TCATTGAGAT TTCTGATAAT 
CGTAGGCGTG ACCCTGATGG GAGAATGCCG TCTTTCTCTC CTGCTCAAGA GGCTTTGTTT 
AGTGTTCATG ACAGGATTTT GGAGAGTGAA GCTCAGTTTG GTTATGGTGG ACCTCCGCCG 
GAAGAGGAGG AGGATTACGG TGGAGTTAGA CCTGGTGGAG GGAGAGTTGT GACTAGATTA 
GTGGTTTCTA GGATGCATGT TGGTTGTTTG TTGGGTAAAG GTGGGAAGAT TATTGAGCAG 
ATGAGGATTG AAACTAAGAC TCATATTCGG ATTCTTCCAA GAGAAAGTAA CTTACCTCGT 
TGTGTTTCAC TCTCTGAAGA GATTGTTCAG ATTGTAGGTG AACTAAATGC AGTGAAAAAC 
GCTTTAGCCA TTGTATCATC TCGTCTAAGG GAGAGTCAGC ATCGTGATCG GAGTAATTTC 
CAAGGGCGTT CACATTCACC AGAACGGTCG TTTGCAGCTG CGGGGGATGA TTACATGCCA 
CAGCTAAGAA GACAATCATC CGATAGGTTT CCTCGTGGTA ATTTTAGAAA TAACAACTTC 
AGTTCTCGCC AGTCCAACTA TGCAGAAGAA GCTCCAGCAG TTCCAGTTGG TGAGAATGTC 
TACTCCGAAG AGCTTGTGTT CCAGATTCTC TGCCCAGCTG ACAAGATTGT TCGGGTTGTT 
GGGGAATCGC AAGGAATAAT TGATTTGCTT CAGAACGAGA TCGGCGTGGA CGTTAGAGTA 
TCTGACCCAG TTGCTGGATC TGATGAACAG ATAATCACCA TTTCTTCGGA AGAGGCACCT 
GATGATCCAT TTTTCCCTGC TCAAGAAGCC TTGTTGCACA TTCAAACTCA GATCATAGAT 
CTTATTCCGG ATAAAGATAA TTTAATAACA ACAAGATTAC TTGTCCCATC TAGAGACTCT 
ATATGTTTGG AAGGGAAAGC AGGATCAGTA TCTGAGATAT CACGATTAAC TGGAACAAGC 
GTACAAATTC TAGCTAGGGA AGAAATTCCC CGATGTGCTT CTATCAATGA TGTTGTTATA 
CAGATTACTG GCGAGATCAG AGCTGCTCGG GAAGCACTTG TTGAATTAAC ATTACTATTG 
AGGAGTCATA TGTTCAAGGA GCTCTCTCAA AAGGAGACAC CACCAGCTTC CACATCCACA 
ACTGGCCCTC TCGAAGGTGT TGCAGGAGTA ATGGAGGTAG CTTCATCAAA TAACACAATT 
CAATCTCGTG AAGGTCCTAC TAGTTCGAAT CTGAACTTGC AGCAAGTTAG CACAATCTTG 
CCACAGTTTA AGGAAGGTTT CGGATCAGTT GCAAAGGCGG GTGAAAGTGA ACACCGGGAA 
GAGGTTCCTG TCACGACAAG CAGAATGGCG GTACCACTTG TTACTAGAAG CACTCTTGAA 
GTTGTCTTAC CAGAGGCGGT TGTTCCTAAG CTCGTTACGA AATCAAGAAA CAAGCTTGCA 
CAAATCAGTG AGTGGTCTGG AGCTAGTGTA ACCATAGTTG AAGACAGACC AGAAGAAACA 
CAGAATATCA TTCGAATTTC GGGTACACCC GAGCAAGCAG AGAGAGCTCA GAGTTTGCTT 
CAAGGCTTCA TTTTGAGCAT ACAAGAGGAT GGACCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RCF3 ( NM_001345032.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001330551.1 Arabidopsis thaliana RNA-binding KH domain-containing protein (RCF3), mRNA.

Price & Availability↑

CloneID	OAb41873
Clone ID Related Accession (Same CDS sequence)	NM_001345032.1
Accession Version	NM_001345032.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	RNA-binding KH domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGGAGAGAT CTAGATCCAA GAGAAACTAC CACTATGACC AAGACTATGA CGGAGATTCC 
ATGCCTAGGT CTAAACCTCG CTACAACAAT AATTACCACT TTGGTGGTGG CGGCGGAGGA 
AATAACAGAT ACCGTGGCGG CGGCGGTGGT GGAGGTGGAA ATGGCCGGCC ATCGAAGTCT 
CATCCTGAAA CGATGGCAAC AACGACTTAT CGAATCCTCT GTCACGACGC TAAAGCAGGA 
GGTGTTATTG GTAAATCCGG GACAATTATC AAATCTATAA GGCAACACAC CGGCGCGTGG 
ATTAACGTGC ACGAGCTAGT TCCTGGAGAT GCAGAGCGTA TCATTGAGAT TTCTGATAAT 
CGTAGGCGTG ACCCTGATGG GAGAATGCCG TCTTTCTCTC CTGCTCAAGA GGCTTTGTTT 
AGTGTTCATG ACAGGATTTT GGAGAGTGAA GCTCAGTTTG GTTATGGTGG ACCTCCGCCG 
GAAGAGGAGG AGGATTACGG TGGAGTTAGA CCTGGTGGAG GGAGAGTTGT GACTAGATTA 
GTGGTTTCTA GGATGCATGT TGGTTGTTTG TTGGGTAAAG GTGGGAAGAT TATTGAGCAG 
ATGAGGATTG AAACTAAGAC TCATATTCGG ATTCTTCCAA GAGAAAGTAA CTTACCTCGT 
TGTGTTTCAC TCTCTGAAGA GATTGTTCAG ATTGTAGGTG AACTAAATGC AGTGAAAAAC 
GCTTTAGCCA TTGTATCATC TCGTCTAAGG GAGAGTCAGC ATCGTGATCG GAGTAATTTC 
CAAGGGCGTT CACATTCACC AGAACGGTCG TTTGCAGCTG CGGGGGATGA TTACATGCCA 
CAGCTAAGAA GACAATCATC CGATAGGTTT CCTCGTGGTA ATTTTAGAAA TAACAACTTC 
AGTTCTCGCC AGTCCAACTA TGCAGAAGAA GCTCCAGCAG TTCCAGTTGG TGAGAATGTC 
TACTCCGAAG AGCTTGTGTT CCAGATTCTC TGCCCAGCTG ACAAGATTGT TCGGGTTGTT 
GGGGAATCGC AAGGAATAAT TGATTTGCTT CAGAACGAGA TCGGCGTGGA CGTTAGAGTA 
TCTGACCCAG TTGCTGGATC TGATGAACAG ATAATCACCA TTTCTTCGGA AGAGGCACCT 
GATGATCCAT TTTTCCCTGC TCAAGAAGCC TTGTTGCACA TTCAAACTCA GATCATAGAT 
CTTATTCCGG ATAAAGATAA TTTAATAACA ACAAGATTAC TTGTCCCATC TAGAGACTCT 
ATATGTTTGG AAGGGAAAGC AGGATCAGTA TCTGAGATAT CACGATTAAC TGGAACAAGC 
GTACAAATTC TAGCTAGGGA AGAAATTCCC CGATGTGCTT CTATCAATGA TGTTGTTATA 
CAGATTACTG GCGAGATCAG AGCTGCTCGG GAAGCACTTG TTGAATTAAC ATTACTATTG 
AGGAGTCATA TGTTCAAGGA GCTCTCTCAA AAGGAGACAC CACCAGCTTC CACATCCACA 
ACTGGCCCTC TCGAAGGTGT TGCAGGAGTA ATGGAGGTAG CTTCATCAAA TAACACAATT 
CAATCTCGTG AAGGTCCTAC TAGTTCGAAT CTGAACTTGC AGCAAGTTAG CACAATCTTG 
CCACAGTTTA AGGAAGGTTT CGGATCAGTT GCAAAGGCGG GTGAAAGTGA ACACCGGGAA 
GAGGTTCCTG TCACGACAAG CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001330551.1
CDS	347..2053
Translation	MERSRSKRNYHYDQDYDGDSMPRSKPRYNNNYHFGGGGGGNNRYRGGGGGGGGNGRPSKSHPETMATTTYRILCHDAKAGGVIGKSGTIIKSIRQHTGAWINVHELVPGDAERIIEISDNRRRDPDGRMPSFSPAQEALFSVHDRILESEAQFGYGGPPPEEEEDYGGVRPGGGRVVTRLVVSRMHVGCLLGKGGKIIEQMRIETKTHIRILPRESNLPRCVSLSEEIVQIVGELNAVKNALAIVSSRLRESQHRDRSNFQGRSHSPERSFAAAGDDYMPQLRRQSSDRFPRGNFRNNNFSSRQSNYAEEAPAVPVGENVYSEELVFQILCPADKIVRVVGESQGIIDLLQNEIGVDVRVSDPVAGSDEQIITISSEEAPDDPFFPAQEALLHIQTQIIDLIPDKDNLITTRLLVPSRDSICLEGKAGSVSEISRLTGTSVQILAREEIPRCASINDVVIQITGEIRAAREALVELTLLLRSHMFKELSQKETPPASTSTTGPLEGVAGVMEVASSNNTIQSREGPTSSNLNLQQVSTILPQFKEGFGSVAKAGESEHREEVPVTTSR

Target ORF information:

RefSeq Version	NM_001345032.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana RNA-binding KH domain-containing protein (RCF3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001345032.1

ORF Insert Sequence:

ATGGAGAGAT CTAGATCCAA GAGAAACTAC CACTATGACC AAGACTATGA CGGAGATTCC 
ATGCCTAGGT CTAAACCTCG CTACAACAAT AATTACCACT TTGGTGGTGG CGGCGGAGGA 
AATAACAGAT ACCGTGGCGG CGGCGGTGGT GGAGGTGGAA ATGGCCGGCC ATCGAAGTCT 
CATCCTGAAA CGATGGCAAC AACGACTTAT CGAATCCTCT GTCACGACGC TAAAGCAGGA 
GGTGTTATTG GTAAATCCGG GACAATTATC AAATCTATAA GGCAACACAC CGGCGCGTGG 
ATTAACGTGC ACGAGCTAGT TCCTGGAGAT GCAGAGCGTA TCATTGAGAT TTCTGATAAT 
CGTAGGCGTG ACCCTGATGG GAGAATGCCG TCTTTCTCTC CTGCTCAAGA GGCTTTGTTT 
AGTGTTCATG ACAGGATTTT GGAGAGTGAA GCTCAGTTTG GTTATGGTGG ACCTCCGCCG 
GAAGAGGAGG AGGATTACGG TGGAGTTAGA CCTGGTGGAG GGAGAGTTGT GACTAGATTA 
GTGGTTTCTA GGATGCATGT TGGTTGTTTG TTGGGTAAAG GTGGGAAGAT TATTGAGCAG 
ATGAGGATTG AAACTAAGAC TCATATTCGG ATTCTTCCAA GAGAAAGTAA CTTACCTCGT 
TGTGTTTCAC TCTCTGAAGA GATTGTTCAG ATTGTAGGTG AACTAAATGC AGTGAAAAAC 
GCTTTAGCCA TTGTATCATC TCGTCTAAGG GAGAGTCAGC ATCGTGATCG GAGTAATTTC 
CAAGGGCGTT CACATTCACC AGAACGGTCG TTTGCAGCTG CGGGGGATGA TTACATGCCA 
CAGCTAAGAA GACAATCATC CGATAGGTTT CCTCGTGGTA ATTTTAGAAA TAACAACTTC 
AGTTCTCGCC AGTCCAACTA TGCAGAAGAA GCTCCAGCAG TTCCAGTTGG TGAGAATGTC 
TACTCCGAAG AGCTTGTGTT CCAGATTCTC TGCCCAGCTG ACAAGATTGT TCGGGTTGTT 
GGGGAATCGC AAGGAATAAT TGATTTGCTT CAGAACGAGA TCGGCGTGGA CGTTAGAGTA 
TCTGACCCAG TTGCTGGATC TGATGAACAG ATAATCACCA TTTCTTCGGA AGAGGCACCT 
GATGATCCAT TTTTCCCTGC TCAAGAAGCC TTGTTGCACA TTCAAACTCA GATCATAGAT 
CTTATTCCGG ATAAAGATAA TTTAATAACA ACAAGATTAC TTGTCCCATC TAGAGACTCT 
ATATGTTTGG AAGGGAAAGC AGGATCAGTA TCTGAGATAT CACGATTAAC TGGAACAAGC 
GTACAAATTC TAGCTAGGGA AGAAATTCCC CGATGTGCTT CTATCAATGA TGTTGTTATA 
CAGATTACTG GCGAGATCAG AGCTGCTCGG GAAGCACTTG TTGAATTAAC ATTACTATTG 
AGGAGTCATA TGTTCAAGGA GCTCTCTCAA AAGGAGACAC CACCAGCTTC CACATCCACA 
ACTGGCCCTC TCGAAGGTGT TGCAGGAGTA ATGGAGGTAG CTTCATCAAA TAACACAATT 
CAATCTCGTG AAGGTCCTAC TAGTTCGAAT CTGAACTTGC AGCAAGTTAG CACAATCTTG 
CCACAGTTTA AGGAAGGTTT CGGATCAGTT GCAAAGGCGG GTGAAAGTGA ACACCGGGAA 
GAGGTTCCTG TCACGACAAG CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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RCF3 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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